LOCUS       DAXDZG010000001        64301 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXDZG010000001 DAXDZG010000000
VERSION     DAXDZG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 64301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64301
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.001"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..120
                     /locus_tag="ACFB78_000001"
     CDS             <1..120
                     /locus_tag="ACFB78_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC1268108.1"
                     /translation="ILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE"
     gene            248..1543
                     /gene="hisS"
                     /locus_tag="ACFB78_000002"
     CDS             248..1543
                     /gene="hisS"
                     /locus_tag="ACFB78_000002"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC1268109.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            1544..2173
                     /locus_tag="ACFB78_000003"
     CDS             1544..2173
                     /locus_tag="ACFB78_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC1268110.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            2323..3780
                     /gene="der"
                     /locus_tag="ACFB78_000004"
     CDS             2323..3780
                     /gene="der"
                     /locus_tag="ACFB78_000004"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC1268111.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3811..4710
                     /locus_tag="ACFB78_000005"
     CDS             3811..4710
                     /locus_tag="ACFB78_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC1268112.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4762..5179
                     /locus_tag="ACFB78_000006"
                     /pseudo
     CDS             4762..5179
                     /locus_tag="ACFB78_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5247..5813
                     /gene="dcd"
                     /locus_tag="ACFB78_000007"
     CDS             5247..5813
                     /gene="dcd"
                     /locus_tag="ACFB78_000007"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC1268113.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5879..6583)
                     /locus_tag="ACFB78_000008"
     CDS             complement(5879..6583)
                     /locus_tag="ACFB78_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC1268114.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGMVQF"
     gene            6727..7922
                     /locus_tag="ACFB78_000009"
                     /pseudo
     CDS             6727..7922
                     /locus_tag="ACFB78_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            7948..8901
                     /locus_tag="ACFB78_000010"
     CDS             7948..8901
                     /locus_tag="ACFB78_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC1268115.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTKDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9006..9329)
                     /locus_tag="ACFB78_000011"
     CDS             complement(9006..9329)
                     /locus_tag="ACFB78_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268116.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(9502..10860)
                     /locus_tag="ACFB78_000012"
     CDS             complement(9502..10860)
                     /locus_tag="ACFB78_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC1268117.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNISAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(11218..12918)
                     /gene="recJ"
                     /locus_tag="ACFB78_000013"
     CDS             complement(11218..12918)
                     /gene="recJ"
                     /locus_tag="ACFB78_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC1268118.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(12970..13701)
                     /locus_tag="ACFB78_000014"
     CDS             complement(12970..13701)
                     /locus_tag="ACFB78_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268119.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILVYAPLVDIGCG
                     GRGAGLAYGSDCSREFLRQMNGNAV"
     gene            complement(13748..14254)
                     /gene="yjgA"
                     /locus_tag="ACFB78_000015"
     CDS             complement(13748..14254)
                     /gene="yjgA"
                     /locus_tag="ACFB78_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC1268120.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RARVRLLADEGALTQFMSDFPNAGAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            14408..15739
                     /gene="pmbA"
                     /locus_tag="ACFB78_000016"
     CDS             14408..15739
                     /gene="pmbA"
                     /locus_tag="ACFB78_000016"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC1268121.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16038..16241)
                     /locus_tag="ACFB78_000017"
     CDS             complement(16038..16241)
                     /locus_tag="ACFB78_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC1268122.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            16486..16788
                     /gene="clpS"
                     /locus_tag="ACFB78_000018"
     CDS             16486..16788
                     /gene="clpS"
                     /locus_tag="ACFB78_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC1268123.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            16790..19081
                     /gene="clpA"
                     /locus_tag="ACFB78_000019"
     CDS             16790..19081
                     /gene="clpA"
                     /locus_tag="ACFB78_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC1268124.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINGRFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(19121..22219)
                     /locus_tag="ACFB78_000020"
     CDS             complement(19121..22219)
                     /locus_tag="ACFB78_000020"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC1268125.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVVVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNIVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(22450..23670)
                     /locus_tag="ACFB78_000021"
     CDS             complement(22450..23670)
                     /locus_tag="ACFB78_000021"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1268126.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEATLEAELALRKRQYRYYRDLLLDFDNQIGGIADGYQCRLKNV
                     VWKTLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVND
                     ILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHA
                     KGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVSEGLPHEIALRRKQY
                     EYYREQLLAFPKAA"
     gene            complement(23837..24688)
                     /gene="dinD"
                     /locus_tag="ACFB78_000022"
     CDS             complement(23837..24688)
                     /gene="dinD"
                     /locus_tag="ACFB78_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC1268127.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(24804..26347)
                     /locus_tag="ACFB78_000023"
                     /pseudo
     CDS             complement(24804..26347)
                     /locus_tag="ACFB78_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            complement(26422..27426)
                     /gene="rfaD"
                     /locus_tag="ACFB78_000024"
     CDS             complement(26422..27426)
                     /gene="rfaD"
                     /locus_tag="ACFB78_000024"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC1268128.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(27490..28452)
                     /gene="hldA"
                     /locus_tag="ACFB78_000025"
     CDS             complement(27490..28452)
                     /gene="hldA"
                     /locus_tag="ACFB78_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC1268129.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(28508..29248)
                     /gene="pyrF"
                     /locus_tag="ACFB78_000026"
     CDS             complement(28508..29248)
                     /gene="pyrF"
                     /locus_tag="ACFB78_000026"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC1268130.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(29772..30419)
                     /gene="adk"
                     /locus_tag="ACFB78_000027"
     CDS             complement(29772..30419)
                     /gene="adk"
                     /locus_tag="ACFB78_000027"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC1268131.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            30634..31473
                     /gene="htpX"
                     /locus_tag="ACFB78_000028"
     CDS             30634..31473
                     /gene="htpX"
                     /locus_tag="ACFB78_000028"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC1268132.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(31709..33007)
                     /locus_tag="ACFB78_000029"
     CDS             complement(31709..33007)
                     /locus_tag="ACFB78_000029"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC1268133.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(33112..34263)
                     /locus_tag="ACFB78_000030"
     CDS             complement(33112..34263)
                     /locus_tag="ACFB78_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC1268134.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSAALLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            34598..35977
                     /gene="norM"
                     /locus_tag="ACFB78_000031"
     CDS             34598..35977
                     /gene="norM"
                     /locus_tag="ACFB78_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC1268135.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHEAV"
     gene            36021..37061
                     /gene="murB"
                     /locus_tag="ACFB78_000032"
     CDS             36021..37061
                     /gene="murB"
                     /locus_tag="ACFB78_000032"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC1268136.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            37073..37723
                     /locus_tag="ACFB78_000033"
     CDS             37073..37723
                     /locus_tag="ACFB78_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC1268137.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            37778..38554
                     /locus_tag="ACFB78_000034"
     CDS             37778..38554
                     /locus_tag="ACFB78_000034"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1268138.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            38604..39170
                     /locus_tag="ACFB78_000035"
     CDS             38604..39170
                     /locus_tag="ACFB78_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC1268139.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            39190..40080
                     /locus_tag="ACFB78_000036"
     CDS             39190..40080
                     /locus_tag="ACFB78_000036"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC1268140.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            40105..40863
                     /locus_tag="ACFB78_000037"
     CDS             40105..40863
                     /locus_tag="ACFB78_000037"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC1268141.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(40918..41582)
                     /locus_tag="ACFB78_000038"
                     /pseudo
     CDS             complement(40918..41582)
                     /locus_tag="ACFB78_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(41779..42552)
                     /gene="folE2"
                     /locus_tag="ACFB78_000039"
     CDS             complement(41779..42552)
                     /gene="folE2"
                     /locus_tag="ACFB78_000039"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC1268142.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            42755..43912
                     /locus_tag="ACFB78_000040"
     CDS             42755..43912
                     /locus_tag="ACFB78_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC1268143.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(43976..44977)
                     /gene="hemB"
                     /locus_tag="ACFB78_000041"
     CDS             complement(43976..44977)
                     /gene="hemB"
                     /locus_tag="ACFB78_000041"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC1268144.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(45161..45445)
                     /gene="yhbY"
                     /locus_tag="ACFB78_000042"
     CDS             complement(45161..45445)
                     /gene="yhbY"
                     /locus_tag="ACFB78_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC1268145.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            45555..46175
                     /locus_tag="ACFB78_000043"
     CDS             45555..46175
                     /locus_tag="ACFB78_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC1268146.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            46239..48206
                     /gene="ftsH"
                     /locus_tag="ACFB78_000044"
     CDS             46239..48206
                     /gene="ftsH"
                     /locus_tag="ACFB78_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC1268147.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            48428..48859
                     /locus_tag="ACFB78_000045"
     CDS             48428..48859
                     /locus_tag="ACFB78_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC1268148.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            48852..49130
                     /locus_tag="ACFB78_000046"
     CDS             48852..49130
                     /locus_tag="ACFB78_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC1268149.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            49183..49761
                     /gene="pth"
                     /locus_tag="ACFB78_000047"
     CDS             49183..49761
                     /gene="pth"
                     /locus_tag="ACFB78_000047"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC1268150.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            49822..50028
                     /locus_tag="ACFB78_000048"
     CDS             49822..50028
                     /locus_tag="ACFB78_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC1268151.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            50025..50387
                     /locus_tag="ACFB78_000049"
     CDS             50025..50387
                     /locus_tag="ACFB78_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC1268152.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            50616..52031
                     /locus_tag="ACFB78_000050"
     CDS             50616..52031
                     /locus_tag="ACFB78_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC1268153.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVKIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            52151..52660
                     /locus_tag="ACFB78_000051"
     CDS             52151..52660
                     /locus_tag="ACFB78_000051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1268154.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            52828..54210
                     /locus_tag="ACFB78_000052"
     CDS             52828..54210
                     /locus_tag="ACFB78_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC1268155.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(54266..55021)
                     /locus_tag="ACFB78_000053"
     CDS             complement(54266..55021)
                     /locus_tag="ACFB78_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1268156.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(55031..55747)
                     /locus_tag="ACFB78_000054"
     CDS             complement(55031..55747)
                     /locus_tag="ACFB78_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1268157.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(55737..56564)
                     /locus_tag="ACFB78_000055"
     CDS             complement(55737..56564)
                     /locus_tag="ACFB78_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1268158.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            56849..57757
                     /locus_tag="ACFB78_000056"
     CDS             56849..57757
                     /locus_tag="ACFB78_000056"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC1268159.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            57834..61436
                     /gene="recB"
                     /locus_tag="ACFB78_000057"
     CDS             57834..61436
                     /gene="recB"
                     /locus_tag="ACFB78_000057"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC1268160.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIAHALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            61475..61834
                     /locus_tag="ACFB78_000058"
     CDS             61475..61834
                     /locus_tag="ACFB78_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC1268161.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            61959..62438
                     /locus_tag="ACFB78_000059"
     CDS             61959..62438
                     /locus_tag="ACFB78_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC1268162.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(62713..64092)
                     /gene="radA"
                     /locus_tag="ACFB78_000060"
     CDS             complement(62713..64092)
                     /gene="radA"
                     /locus_tag="ACFB78_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC1268163.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADASTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT        15055 a        18409 c        16304 g        14533 t
ORIGIN      
        1 attttggcgg tcgtgccgaa aaaagcggag gcgatttcaa ataaattgtt tttcacacat
       61 catcccaagc ggcagacgga gtttgcagtc ggacaaatca ggcaagggcg cgcagagtaa
      121 gtcaaatgcc gtctgaaaac tgtttcagac ggcattttta cccgtccgca aaccatctcc
      181 atcctcacaa gcccttaaaa atccgctata atcgcccgca atttgatttc agcaccccct
      241 ttttgaaatg gcacaaaaaa tccaatccgt caaaggcatg aacgaccttc tgcctgtcgg
      301 gcaaaaagat ttcaaactca cggctgtgtt ttggcaggcg tttgaggata cggtaaaccg
      361 ttggacgcga gcttacggtt atcagcaaat ccgtacgccg attgtcgagc aaaccggttt
      421 gtttgtccgc tccatcggcg aggaaaccga tgtagtcggc aaggaaatgt acaccttctc
      481 cgattcaaac gattctttga gtttgagcct gcgtcccgaa ggtacggcct cttgtctgcg
      541 tgcggtggtc gaacacaacc ttctgtacaa cagcccgcaa aagctgtggt atatggggcc
      601 gatgttccgc cgcgagcgtc cgcaaaaagg acgctaccgc caatttcatc aggtcggtat
      661 cgaggctttg ggttttgaag ggccggatat tgatgcggaa atcatcgcaa tgtctgccga
      721 cttgtgggaa aaattgggta ttcgcgaata cctgactttg gaaatcaaca gcttgggcaa
      781 ccgtgaggaa cgcgcggcac accgtgcggc attggttgaa tatctgaccc gctatgaagc
      841 gcaactggac gaagacagca aacgccgtct gaaaaccaat cctttgcgcg ttttggatac
      901 gaaaaaccct gatttgcagg aaatctgcaa tgccgcgccg cgtttggtag attacttggg
      961 cgaggcttcg caaaaccact atgcacgctt caaggcgatg ttggacggtt tgggtattca
     1021 atatattgaa aattcgcgct tggttcgcgg tttggattat tacaatcaga cggtttttga
     1081 gtggacgacc gacaaactcg gcgcacaggc gactgtgtgc ggcggcggac gttacgacgg
     1141 tttgattgaa gaactcggcg gcaagcctgc gccgtccatc ggctttgcga tgggcatcga
     1201 acggctgctg cttttggtga gcgaatacgg ttcgctggaa gtgaacgccg cgcctgatgt
     1261 ctatgcaatg caccaaggcg aaggggcgga cttgcaggtg atgaaatacg cacaagcctt
     1321 gcgcgcgcaa ggtttcaatg taatgcagca ttccggctat caaagcctga aggcgcaaat
     1381 gaaaaaagcc gacaacagcg gcgcgcgctt tgccctgatt gtcgcgcaag acgaactggc
     1441 ggacggtacc gttacgctca aagacatgaa cggcgcacac ggtcagcaaa ccgtttccgc
     1501 caccgatttg acagatactt tacaacaatg gaagaacgca taaatggcag cccatctcga
     1561 agaacaacaa gagttagaca actttaaata tttttggaaa accacgggca aatggctgtt
     1621 tgccctgctg attttggcgg cactcggcta cttgggatac acggtttacc aaaaccgtgc
     1681 ggcttcccaa aatcaggaag cggcggcggt gctggcaaac atcgtggaaa aggcgcaaaa
     1741 caaagccccg caaagcgaaa tcaatgccga actgtccaag ctccaacaaa gctaccccca
     1801 ttccatttcc gccgcccaag ccacgctgat ggcggcggca accgaatttg acgcgcagcg
     1861 ttacgatgtt gccgaaggtc atttgaaatg ggtgttgtcc aaccaaaaag acagcctgat
     1921 tcaggcgttg gcggcgcagc gtctgggcgt tgtgttgttg caacaaaaaa aatacgatgc
     1981 cgcgcttgcc gcactcgaca cgccggttga ggcggacttc gcccccctgc tgatggaaac
     2041 taaaggcgat gtttatgccg cacaggaaaa aagccaggaa gccttaaaaa actacggaca
     2101 ggctttggaa aaaatgcctc aagattctgt cggtcgcgaa ttgcttcaaa tgaaactcga
     2161 ttcgctgaaa taaataccgt ctgaaatgcc gctctctctt cagacggcat ttcagacaac
     2221 cccatcctac ggcaattccc gtaaaaccat ccgccgtgtc ccaacggaca cctgctcgtg
     2281 ttcagttcta ccgaacggca cttgacacag aaagacccaa tcatgaaacc aaccatcgcg
     2341 cttatcggcc gccccaacgt cggcaaatct accttgttca accgtttgac gcgcaccaaa
     2401 gacgcgctcg tgcatgacct gccgggtctg acccgcgacc gccattacgg acacggcaaa
     2461 gtcggcagca aaccttattt tgtcatcgat accggcggtt tcgagccggt tgtggacagc
     2521 ggcattttgc acgaaatggc aaaacaaacc ttgcaggctg tcgatgaagc cgatgcggtt
     2581 gtgtttttgg tggacggccg taccggttta acaccgcaag acaagattat tgccgaccgt
     2641 ttgcgccaaa gtccgcgtcc cgtttatttg gccgtgaata aaggcgaggg cggcgacagg
     2701 gccgtacttg ccgccgagtt ttacgagctg gcattgggcg agccgcacgt tatttccggc
     2761 gcgcacggcg acggcgtgta ttacctgatt gaagaaattt tagaaaactt ccccgagcct
     2821 gaagccgaag aagccgatgc aaaacatccc gtttttgccg ttatcggtcg tccgaacgtc
     2881 ggcaaatcta cgctggttaa cgccattctc ggcgaaaaac gcgtcatcgc cttcgatatg
     2941 gcaggcacaa cgcgcgacag catccacatc gatttcgagc gcgaaggcaa accgtttacc
     3001 atcatcgata ccgcaggcgt gcgccgtcgc ggcaaagtgg atgaagcggt ggaaaagttc
     3061 tccgttatca aagctatgca ggcggttgaa gcggcaaacg ttgctgtttt ggtattggac
     3121 gcgcaacagg acattgccga ccaagatgcg acgattgccg gttttgcttt ggaagcggga
     3181 cgcgcgctgg tagttgccgt caacaaatgg gacggcatca gcgaagagcg gcgcgaacaa
     3241 gtgaaacgcg acatttcccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt
     3301 tccgcgttga aagagcgcgg tatagacggt ttgtttgaaa gcattcaagc tgcctacaac
     3361 gcggcgatga ttaagatgcc gacgccgaaa atcacgcgcg tattgcaaac cgccgtcgga
     3421 cgccaacagc cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc
     3481 ggcatgaatc cgccggtgat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc
     3541 tatacgcgct atctgaccca gaccttccgc aaagccttca acctgcaagg cacgccgttg
     3601 cggattcaat acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa
     3661 aaaccgctgc gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgaaaggaa
     3721 gagaaaaacc gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa
     3781 ttcccgacat ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg
     3841 ctcaacaaag aaaagctgcc tgaggcggac gtacttgccg ataaacttgc tgaagctgaa
     3901 tttacccatt gccaaggttt ggactggttc agcgaaggct ttaccgcacc ggtttctttt
     3961 tcgcccgaac tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaggaa
     4021 aaagtcctgc ctgccggcgt catccgcgat attttggaag agaaggtagc ggaaatccaa
     4081 aacaatgaag cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca
     4141 gacgacctgc tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc
     4201 cgccacggct acctgctcgt caataacgcg gcttccgcca aagcggaaaa catcctgacc
     4261 aagctgcgcg aagctttggg aggtttggaa gcctcgctgc cgaataccaa acaatcgccc
     4321 tcttccctga tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattagac
     4381 agcgattgcg aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa
     4441 gacttaaccg ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt tacccggctc
     4501 ggtttggttt ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc
     4561 atccaatacc tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccggc
     4621 cttgccttcg cctcgcaaat cctgatggcg gaatccgtca gcaccatgtt ggaagagctg
     4681 gtttcctatt tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca
     4741 aaatccaatg gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg
     4801 ttcgccctcc ccgcccagac cgccgtccta agcccctatc aggaaaccgg ctgcacctac
     4861 gaaggcggga tcggaaaaga cgggcttcct tcaggcaaag gcatatggcg ttgccgggat
     4921 gggcgcggtt ataccggttc attcaaaaac ggcaaattcg acgggcaagg cgtttatacc
     4981 gttgccgccg gccgcgaagt atttctcgag ccgttcaatt ccgacagtac caaattccgc
     5041 aatatggcat tgtcgggcac gttcaaacaa ggcttggcac acggcaggtt cgccgcctcg
     5101 caaaacggcg aaaccctctt ttattatgaa atgcgaacac ggcatgatta aagaagtgaa
     5161 actgcccaaa aacaaataac ccccccgttt ttcaaacagc atcccccaat gctttatccg
     5221 aacaccgcaa aaaacaagga aaccccatga gcatcaagtc cgacaaatgg atacgccgaa
     5281 tgagcgaaga attcggcatg attgatccct tcgagccgaa ccaaatcaaa gaagccgacg
     5341 gacagcgcat catctcctac ggtacgtcca gctacggcta cgatatccgc tgcgccaatg
     5401 aatttaaaat ttttaccaac atcaacagca ccatcgtcga tcccaaaaac ttcgacccga
     5461 aaaatttcgt taccgtcgaa gacgactgct gcatcatccc gcccaattcc ttcgcactgg
     5521 cgcgcacggt cgaatatttc cgcattccgc gcaacgtcct gaccgtctgt ttgggcaaat
     5581 ccacctacgc ccgctgcggc atcatcgtca acgttacccc gttcgagccg gaatgggaag
     5641 gctacgttac cctcgagttt tccaacacca cccccctgcc cgccaaaatc tacgcaggcg
     5701 aaggcgtggc gcaagtcctc ttcttcgaga gcgacgaagt gtgtgaaact tcatacaaag
     5761 accgcaacgg caaatacatg ggacaaaccg gcgtaaccct gcccaaagcc tgaaatacaa
     5821 aatgccgtct gaacccttat cgtcgggttt cagacggcat ctacctattt atccgtggtt
     5881 agaactgaac catcccattg atgctgatgc tgatttcctc cacacccggt gcggctgaat
     5941 ccgtaccctt catattgacg cttgccgcca tcggcatcgc gcgcagcatt tttgcccgaa
     6001 cagccccatc gcccgcaata tggctgccga tttgcccaaa attcaatttg acgattttat
     6061 aaccggacgc acccagaacg cccgccagtt tttcggcacg cgccttgaaa cgcaaaacgg
     6121 catccttgct gacctgatcg atgacctcgt ttcggcgttc gcgcgacacg ctgaaatcgg
     6181 tatcttcaag cgaagcatcc gtctgaacat cagcaataaa acggtttaaa gcatcaaaat
     6241 ccctgccctc cgccttaaat tccgcacgct cctcccagcc tgtttgaatg cgtctgccgt
     6301 tggtatattg atagcgcggc atcgcactgc gcgataccaa ttcggtttta aagctaccat
     6361 ttttcgattt tctggtgaaa ttgttgaatt ttttaacaaa ctcggcattg acggcatttt
     6421 tgtcccgtcc ttccgccgcc acctggaaac gcgcggacat tgtatcctga gccacctcga
     6481 tacccgccga ttcggaaaat tcgacaatat tgtaattcaa tgcctcagcc gccgccggaa
     6541 aagataccgc cagcagggaa gccgccaaaa tagaacgcag catatccgta tccttacttg
     6601 aaaaaccgcc aatctaacat ataggtacgc taaattgcgt tagcataaga caacaattta
     6661 aatattcgat cggtctgcct tataataacc gtctttccga tactgaatca tattatgtcc
     6721 gaaccgttgc acgtcctcgt catcccctta tggtatccac gatccgaaca ggatgtggac
     6781 ggaatatttt tccaaaatca agcacaggcc ctccaacgca aaggcatcaa aaccgccatc
     6841 ctcgccccca tgttccgtta tctgcggaaa gaaacagcaa gcatcctgac cggtccttac
     6901 ggttttgccc gataccggca aagcggtttg gacatctacg catggcgcag catgtatttc
     6961 ttccccgctt tccgtttatc gacatcgacc gcatccgctg ggtgcgcgcc ggtttgaaag
     7021 ccttcaaaca ctacatccgc gaaaacggac tgcccgacct gattcacgcc cactgcatga
     7081 actatgcagg catacttgcc caaaaaattt ccgaaaaata cggcattccc tacgttttaa
     7141 ccgaacacag cagcaccatc acgcgcggtc tgatccgtca ccaccaatgg cagccgatgg
     7201 aaaaagcagc ggcgcccgcc tccgcacgtc tcgccgtcag ccgccatttc gcacacgtcc
     7261 tgcaacacaa atacggctgc gaatggcagt acctccccaa cataccgggt ggaatattca
     7321 agcaaacatt tgaataaaaa gaaaaaaata acaaaccgca tttcgtgttc tgcaccgtct
     7381 cgcaccttcg ccgcctcaaa ggacacgatg tcctgctcac tgcttttgcc cgggcgttgg
     7441 cacaatgccc gcaactgcgc ctgaacatcg gcggcagcgg acaggaagaa cagcggctga
     7501 aacagcaggc ggcagacttg ggcatcgccc atgccgttac atttttaggc gcactgcaac
     7561 ccgaagcaat cttggatttg atgaggaaca gcgacgcctt catcctcgcc agccgcaccg
     7621 aaaccttcgg cgtggtctat atcgaagccc tctcgcaagg gttgcccgtc atcgccatac
     7681 attgcggcgg cgcggaatcc atcgtttcag acggcaacgg atacctcgtt tccgttgacg
     7741 atgccgatgt cctagccttt ctcaaaatat atgaacacca tgccgatttt tccgccgaac
     7801 agctcagaac cgactgcctc gcaacattcg gcgaagatgc cgttatcggc agactgattg
     7861 ccgtattccg tcaggcggcg cggaacacgg cgcgaaaacg cccgaaaaat agattaaaat
     7921 gacgcaaaac aaccttcgga ttcaaatatg acccataccg tccacctgca cttggaagaa
     7981 accgacaacc cgacgctgca acgcctgtgc ggttctttcg acagcaacct cgattcgctc
     8041 gccaaagcac tcgacatcca tatcagccgc cgtttcgagc attttacctt caacggtgca
     8101 tttgcacacg ccggcaaacg cgcactgctc aaactcttgg aaacggcgca gacgcgcgac
     8161 ctaaacgaca gcatcatccg ccttgccgcc gtcgaaaccc aaaccaaaga tgccgggcat
     8221 caggaaaaaa atcacgaaca cgcctattat ttccgaacca agcgcggcag cataggcggc
     8281 agaacgccac ggcagaacgg ctatatccgc gccctgctcg accacgacat cgtattcggc
     8341 ctcgggccgg caggtacggg caaaacctat cttgccgtcg ccgccgccgt cgatgcgatg
     8401 gaaaaacacc aagtcgaacg catcatttta gtgcggcctg ccgtcgaagc cggtgagaaa
     8461 ctgggcttcc tgcccggaga cctgacccaa aaagtcgacc cctacctccg tccgctttat
     8521 gatgccctct atgacctgat gggctttgac cgtgtaacca agctgattga aaaaggcctg
     8581 attgaaatcg ccccgctcgc ctatatgcgc ggcaggacgc tcaacggcgc atacatcatc
     8641 ctcgacgaag cgcaaaacac cacgcccgaa caaatgaaaa tgttcctgac ccgcatcggc
     8701 ttcggcgcga aagccgtcat taccggcgac accagccaaa tcgacctgcc caaaaacatc
     8761 aaatcgggat tgaaagacgc acgtgagaaa ctgcacggcg tggcaggact gtatttccat
     8821 accttcaccg gcgaagacgt tgtccggcat cctctggtgc aaaaaatcgt cgaagcctac
     8881 gaatcggcag aacacgactg acactccaat gccgtctgaa acccgatgca tggaaaatcg
     8941 ggcagagaaa aacagagacg gaagccaagc tccgtatccg tttctttatc cgtccaaccg
     9001 atacatcaat cgaattttct gcatcggatt gcctcatctc cgcaaaacat tgccgaagtt
     9061 tcgcggttgc acgcctgacg cactttctgc cgcgccacgc cttcattccg atcgcttgcc
     9121 tcaaacgtct tctgaaaagg cgtaacgctg catacataca tggtggaatt tgaaccgaaa
     9181 aggtcgattt tcacggtatt gccgccgctt tccaatacgg cgacacggtg ttccaactgc
     9241 gccagacgtg cttccaaatc gctgattctg tcggcaaatg caggcatggc ggcacacgcg
     9301 gtcaaaatgc ccaaaataca ttttttcatc aatttttcct tgttgcttga accctgccca
     9361 cccggacatc ctttttccgg gcggcagggt cagactttat ttgcagggat gaagcccctt
     9421 acaaatcaga ataatacatg aatcggcact ttatgcttca tcctatgtgt tgaaacttca
     9481 ttgctccttg tctgttgacc gttattccca atccgttccc acaaccttcg gcttgggttt
     9541 gcgcctgcga cggcggcgtt ttttcgcctg tccttcgttt ttttcgtgcc gggcggcggc
     9601 ctcgtttttg gtcatttccg tccgctgttc cgccgatgcc gtctgaaacg ccgtccacca
     9661 ctcggcaagt gcgcggtccg cattgccggt ttcggcgcgc aagagcagga aatcataggc
     9721 ggcacggaaa cgcgcctgtg caaacagttt gtgcggacgt gcgcctttgc ggttttcaaa
     9781 ctgcggctgg aacatccaaa tttcacgcat cgtggcggaa aagcgttgcg gtacgcccca
     9841 accgcgttcg acggtttcgc gcatcgtatt gattgcatcg gacagggcgg gcgtaggttt
     9901 caaaccctgt tgcagattgc ttttccaatg gcggtccaac tctggccaca tcagggccgc
     9961 cagtacgaaa ccgaccgaaa ccgatttgtc ggcacgcagc cgctcatcgg tatttttcag
    10021 ggcaagcgcc gtcatttttc cggcgatgcc gtctgaaacg cgcaaagcat tgagcagcgg
    10081 atggatgtcc ggtatgtcaa atccgttcaa acgtttcaga cactcgcgcg cgtgccctga
    10141 aaacagcagt ttcatgattt cgtcgaacag ccttgccaca ggttcgtgct tcagacggca
    10201 tatcgattcg gcaatcggtg cggcggtttc ttccgacagc tcaaagccca atttgcccga
    10261 caggcggatg gcgcgcaaaa tcctgacagg gtcttcctga tagcgttcgg cggcattacc
    10321 aatcataacc agcctgcggg cggcaacatc ggcaatcccg ttgtggaaat ccaaaatctc
    10381 ttctttttca ggatcgtaat acaaggcatt gcaggtaaaa tcgcgccgca tcgcatcttc
    10441 ttcgatgctg ccgtaggtgt tgtccttcat gattctgccg tgcgcgttct gatgaacctt
    10501 cgcaccgccg cgaaacgtcg ttacttcgat aatctccgca ccgttcatca catggacaat
    10561 ctgaaaacgc ctgccgatga tgcggctgcg gcggaagagt ttgtgcacct gttcgggcgt
    10621 ggcatcagtt gcgacatcga aatctttggg ttcgatgccg agcagcaggt ctctgaccgc
    10681 accgccgacc acataagcct gaaaccccgc ccctttcagg cggcgtatga cgttttcggc
    10741 ggcaaagctc aacatttcgg cactgatgtt gtgtctttcg gcaggaatga ccgttttact
    10801 ttccgctttt ttactgctcc gaccggaagg cagcatctta ttcaaccatt ttttcagcat
    10861 aaatattctg acccgaagag cggcatctcc acgggcaacc gtgttcagac tgcatcaggc
    10921 ggtacgattc cacaagatgc cgaaaaccat aagcaaaagg cgtagaaact acgccgtccg
    10981 aatcacgccg ccctcgcggc ggcaacgcgt cattataaca gattgcccct taaaaaatca
    11041 gaccctgctt ttgtggcgga gtctgaaatt tgatgcacct tatatattgc gtccctaaga
    11101 agggatgatt aacaaaaatt aacacccttt actttctttt ccgattggga acattcccgt
    11161 cgtacaaaca agcaaatgaa ggaacagaaa tcacattcaa acggcgttcc gccgcctcta
    11221 cgccgcttcc cagtagtcga tataaagctg caattcgagg ttgtcgcgcc attcgttggc
    11281 aacggggcgg tacaccgtgc ggatgtattc gggaatgtct tcgctgcaac gccagaacat
    11341 cgcttcaaat tcgcagccgt ctttttgcaa ccacgctttt ttgtgtttgc cttccgcgcc
    11401 caaaggctgc tggcgaacga cgtggaactc gtcggtaaag ctcggcggcg caaagccctg
    11461 cccccaaacg tggcaggcga ggttttgcgc ctgttccaac gtgatgtcgc aggcgggcag
    11521 gctgccgtca gtaatatagg tttgcgacaa atcgtcttcg cacaccatct cacgcacggc
    11581 ttcttcaaag gccgtctgaa acgcgggaat attgtgttca agtatgctta aacccgccgc
    11641 catcgcgtgt ccgccgaatt tcaaaatcaa atcgggatgg cgtttggaca ccaagtccaa
    11701 agcatcgcgc aagtgcagat tgggaatgga gcgtcccgaa ccgcgcactt cgccgttgtc
    11761 cgccggcgca aacacgatgg tcggacggta aaaacggtct ttgaggcggc tggcaacaat
    11821 gccgaccacg ccttgatgga agtcgtcgcg atacgccacc aaagtcgtct gacctgaagg
    11881 cagggtttcg ggaaaggcgt tcaacgcgtc tcgcagcata gactgctcga tttcgcggcg
    11941 ttcgatattg aggttgttta accgagccgc cagttcctgc gcttcggaat catctcgcgc
    12001 caacaggcag gcgatgccga ccgacatatc gtccagccgt ccggcggcgt tgatgcgcgg
    12061 gcccaacgca aagcccatat caaacggctg agccttgcgc caatcgcgcc gcgccacttc
    12121 aaacaaggcg cggatgccgg ggcgcatttt gcccgaacgc atccgtttca aaccttgcga
    12181 cacgaggatg cggttgttgt ggtcgagggg gacgacatcg gcgacggtac cgagtgcgac
    12241 caaatccaaa aggccgccca gattcggctc tttgatgccg tctgaaaaat aattgcggcg
    12301 gcgcagttcg gcacgcaacg ccgtcaacac ataaaaaatc acgcccacgc ccgccaagct
    12361 tttgcttgga aaaccgcagc ctttttggtt cggattgacg atgatgcagt cgggtacggt
    12421 atcggctggc aaatggtggt cggtaacgat gacatccaaa cccagagcct gcgcgcgcgc
    12481 cacgcctgca atactggcaa tgccgttatc caccgtaatc agcaaatcca cgccttgcgc
    12541 ggcggcgatt tcggcgagtt cgggcgttaa gccgtagccg tgctcaaagc ggttgggcac
    12601 gaggaaatcc actttcgcgc ccatcgccgc caaaccgtcc aaaccgacgg cacacgcggt
    12661 cgcgccgtcg gcatcatagt cggcgacaat caggattttt tccttgcgtt caaccgcatc
    12721 cgccaaacgg ccggcggcgg cttcgcaatt cgtcagcgat tgataaggca ggagggaagc
    12781 gagtttgtcg tccaactcgg caggactttg cacaccgcgc gaggcacaaa gccgggcgat
    12841 taaaggatcg gcgccggcgg cgagcaaatg attgaaaacg tcggtattga cggggcgggt
    12901 ttggattttg actgacatag cgcggggttt tacagtgcgg gtgtgcttga aatgccgtct
    12961 gaaggctttt cagacggcat tgccgttcat ttgtctgagg aattcgcggc tgcaatcaga
    13021 gccataggca agaccagcac cgcggccgcc gcatccaata tcaacaaggg gggcgtatac
    13081 taagatattt ccaaacagct tggatttgtc ggtatgtttt tcagtaaccg tataataaat
    13141 atcggcgggc acactttgct caaaatgata atcggcgttc agtttttgcg gtgtggcgta
    13201 gtatttgcct ttggcggata cgcagcgcgt gtaaatggtc cgattgtcga gtttgaccgc
    13261 tttaaactca agctgtttca gcttggcgat gtcgtcaggt ctgccggtat catagcgcag
    13321 gcaaagacct ccggtactga aattctggct gccgggcgct tcgaatttga ccggcagggc
    13381 ttggtggcgg gcatagctcg gggtatcctc aactatttgg aagggcttgt ccaacccggc
    13441 cttcaaaagg cccgtcagct tcgccgaatc ttcgggattg acggcgaacc agtatttccc
    13501 gcccatcatc accaggctgc ccttttccaa ttgggcattg tcttcggcaa ccacaccgaa
    13561 ggcgcggatt tggtctttgt caacgtgttt gcgggtgatt gtttggctga ccgggttgtt
    13621 catcccccgc aacatcatcg tacagccgtt cagtatcaag gttgccgcca aaacggcggc
    13681 ggtcgtttta cggaacaaca ttttatcttc caatctaaat aacggcggat gccgtctgaa
    13741 aatatgccta aatttccgcg cccccgtttt ccatcacggt tttcaattct tgaaacaggg
    13801 cgcggaagtt tttcggcggt ttgtcctgtt cctgttcttt tttggtgttg cggacgagcg
    13861 tcctcagcct gcccgcgccg gcgtttggaa aatcggacat aaactgcgtc agcgcgccct
    13921 cgtctgccaa caaccgtaca cgcgcccgtt ccacgcgttg caaaaaggcg ttgtgcgccg
    13981 catcgtcgcc gcgcagcttg gcaaggaacg cctcgatggg cgcgggatcg gtatcgcgca
    14041 tcagacgtcc gataaattgc gcctgacgtt tgagcgcgcc gttggacgta atttttttat
    14101 aggtaacgac ggcctcgtac aaatcttcat ccaaaccgat ttttttcagc gtgtcgtttg
    14161 agagcctggt cagttccata cccaaatcct gcaaaccgtt catctgcttt ttcatttggg
    14221 ttttgctgat ccactcgtct tcttgttcaa acatcttgat tctcgaattt tttccaatag
    14281 cgtattttaa cagaatgccg ccgcgttcag acggcttttt gcaccgcacg ccctgcaagc
    14341 ggcacgcttt tgcccctgcc gcccgaaacg gttaaaatgc cgcaacgctc caccaagaaa
    14401 gattgttatg ctgttcaacc acaccgcttc cgaactcctc gacctctgcc gccgcacgct
    14461 cgacttggca aaagcagcgg gcgcgaccgc cgccgaagcc gacttcagcg aatcgctcgg
    14521 acaaagcgtc agcgtgcgct tgggcgaaat cgaacaaatc gagttccagc aggacaaatc
    14581 gctggacatt accgtttacg tcggcaaacg caaaggccgc gccggcaccg ccgacttctc
    14641 cgaacaagcc ctgcgcgaca ccgtccgcgc cgccgccgac atcgcccgcc acaccgccga
    14701 agacggttgc gccggacttg ccgaccccga gttgatggcg caacacatcg gcgaccccga
    14761 cctttaccac gaatgggatt tggatacgga agccgccgtc ggcttggcaa aacaatgcga
    14821 acaggcagcg ttggacacgg acagccgcat cgaaaactcc gaaggcgcgg cggtgcaaac
    14881 cggacactac caatacgttt acggcaacac ccacggtttt gccgcacacc ggcaaagcac
    14941 gcaccacagc atttcctgca gcgtcgttgc cgccgacgaa aacggaatgc agcgcgacta
    15001 ctggtacgat tccgcctgcc gccatccgga tatggacagc cccgaaacca tcggtcaaac
    15061 cgccgcccgc cgcactttgc gccgactcgg cagccgcagc atcccgacag gcagctaccc
    15121 cgtcctcttc gataccaccg tttcgggcgg gctgatcgga cacctcgtcg gcacactctc
    15181 cggcggcgcg ctctaccgcc aaagcagttt cctgatcgac agcatcggca aaaaagtcct
    15241 gcccgatttc ctcaacctgc gcgaagaacc gcacatcccc cgctctttcc gcagcagcta
    15301 ttttgatgcg gaaggtgttg ccaccgcgcc gcgcttcgtg attcaaaacg gcattgtcga
    15361 aggctatttc ctcggcagtt acagcgcgcg caaactcgga atgcagacca cgggcaacgc
    15421 cggcggcgcg cacaacctgt atttgaacca tacgcacgaa acgcaatccg acctgctgaa
    15481 agaaatgggc acgggattgc tggtaaccga attgatggga cagggtgcga acaccatcac
    15541 cggcgactat tcgcgcggcg cggcgggttt ttgggtggaa aacggcgtga ttgcctaccc
    15601 cgtccacgag attaccgttg ccggacgctt gcaggatatg taccgcgaca tcgtcggcgt
    15661 ggcggatgac gctttgcggc gttcgtccaa caaaatcggt tcgattctga ttgcggggat
    15721 gacggttgcc ggaagctgaa cgccctgccg atgccgtctg aagacgaagg cggcgcgttt
    15781 tgccccgtgc ctgcccgttt gccgttttat gccgcaccgc cttttaccgg cgacattgca
    15841 catttttttc atcagcactg attttccctt atccgtcaaa atactaagcc acttgcccac
    15901 aacagggtac acacaagttc aggataaggc gggcggggat tggggacttt tccgccatcg
    15961 gcaaaacggc agacaaaaac ggcgggacat tgcctgtccc gccgttttca aatatacatt
    16021 tgaccgtaca tcaggaatta agctgcctga atgttggcgg cctgtttgcc tttagggccg
    16081 gtggttacgt cgaaagagac gcgttggcct tctttcaggg ttttgaaacc ttccatattg
    16141 atcgctgaga agtgtgcgaa caaatcttcg ccgccttcgt caggagtgat gaaaccaaaa
    16201 cctttagcgt cgttaaacca ttttacgata ccggttgcca ttagaaactt cctatactta
    16261 aaaattaaca aaatcagcaa aataaggcat acagcaaact taaacgttca gcgtttccac
    16321 ccagctttat atacttggtc gagtgccgac ttttgcttaa ccgtcttttt acatttgtta
    16381 agacaaaacg tcaagctatg ttttcaaaaa agtgggaaaa taggcgaaag taccgaaaat
    16441 acaacaaaaa tgccgtctga acgcggcttt caaatgggac acgctatgac cgcgcaacac
    16501 caatccgaca cgcttttgca ccgcctgaac accctgccgc cgaaacgtta cggcgtattt
    16561 ttattgaacg acgattacac cacgatggaa tttgtcgttg aaatcctgac cgaaatcttt
    16621 atgctcgggc aggaacaggc ggtagcggta atgctctcgg ttcatcacga aggcaaaggc
    16681 ctgtgcggca cttacacgcg cgatatcgcc caaaccaaac agcaacaagt catgcagcgg
    16741 gcaaaagccg aaggacatcc gctgcaatgt attgtcgagg agatttaata tgcttgcccc
    16801 tgaattggaa cagattttgc agcagcttta ccgcgaggcg cgtaaggctc attatgaatt
    16861 tatcagcctc gagcatctgc ttttggtact catcgaagaa gatgccgccg tgccgaacgt
    16921 cttaaaactc tgcggtgcgg atttaaaagc ggtgtccgaa cagctcgccg ccagcgttgc
    16981 cgaaaacacc cccctgattc ccgaccacct tttagacacg gtcgaaaccc ggcccacgct
    17041 cggcttccaa cgcgtcatcc agcgggcgat ggtgcatacc caatcggcag gcaaaggctt
    17101 ggccgagcct ttggacgttt tggtcgcgct gatgagcgaa accgacagcc acgccgtcta
    17161 tttcctcaag ctgcaatcgg ttacgcgttt tgaagttttg cgctgtattg cccacggctc
    17221 tcccgatgaa gatgaagacg atggcaacta tttttcagac ggcatggacg acgataatga
    17281 gaaccgcacc aaaccgggca aaaacccttt atcggcgtac accgtcaacc tcaacgccga
    17341 agtcaaagcc ggccgtatcg atcctttgat tggtcgcaaa cacgaaatgg aacggctggt
    17401 gcaaatcctg tgccgccgcc gcaaaaacaa tccgcttttg gtcggcgaag caggcgtggg
    17461 caaaaccgcg ctggcggacg gtttggcaca tcaaatcgtc aacgacgaca ttcccgacgc
    17521 gctcaaagag gccgaagtgt acgcgctgga tatgggttcg cttttggcgg gcacaaaata
    17581 ccgcggcgac tttgaagcgc gggtcaaatc cgtcttgaaa cagctcgaaa aaatcccgca
    17641 cgccatttta ttcatcgacg aaatccacac catcatcggc gcgggcagca ccggcggcgg
    17701 cacaatggac gcgtccaacc tgctcaaacc cgcattggcg aaaggttcat tgcgctgtat
    17761 cggcgcaacc acttacgacg aataccgtac tatttttgac aaagaccacg ccctaagccg
    17821 ccgcttccag aaaatcgacg tggtcgaacc caccgttgcc gaaaccgttc aaatcctgcg
    17881 cggcttgaaa ccgatgttcg aagccttcca ccaagtccgc tacacgcaag gcgcactcga
    17941 agccgccgcc gaactctccg cccgctacat caacgggcgc ttcctgcccg acaaagccat
    18001 cgacgtaatg gacgaagcag gcgcggcgca acggattctg cccaaatcca aacagaaaaa
    18061 agtcatcggc aaagcgcaaa tcgaaaccgt catcgccaaa gtcgcgcgga ttcccgaaaa
    18121 gaccgtgtcg cacgacgaca aacaagtgtt gcaattcctc ggccgcgatt tgaacaatat
    18181 ggtttacggg caggaagacg ccatcgacgc gctggtttcc gccgtcaaaa tgtcgcgttc
    18241 gggcttgggg ctgcccgaca aaccgatagg cagcttcctc ttctccggcc ctaccggcgt
    18301 cggcaaaacc gaagtcgcca aacagcttgc ctactcaatg ggcgtaccgc tgcaacgctt
    18361 tgatatgtcc gaatacatgg aacgccacgc cgtatcgcga ctcatcggcg cgccaccggg
    18421 ctacgtcggc tttgaacaag gcggcctttt gaccgaagcc gtcaacaagc agcctcattg
    18481 cgtgttgctc ttggacgaaa tcgaaaaagc ccatccagat attttcaacg tcctcctgca
    18541 agtcatggac gcaggcaaac tgaccgataa caacggtaag agcgccgatt tccgcaacgt
    18601 catcctgatc atgaccacca acgcaggcgc agaaagcctc agccgaccca gcctaggctt
    18661 taccgccaaa cgcgagcgcg gcgacgaaat gcaggcaatc aacaaactct tcacgcccga
    18721 gttccgcaac cgcttggacg cgatcatccc gtttgcacct ttgtccgaac ccatcatcgt
    18781 caaagtcgtg gacaaattcc tgctccagct cgaacaccgg ctcctcgaca aaaaagtcga
    18841 agccgaattc acaccggcat tgcgtaaata cttggcagaa aaaggtttcg acccgcaaat
    18901 gggcgcgcgc ccgatgcacc gcctgattca ggaaaaaatc cgcaaaccgc tcgccgacga
    18961 actcctgttc ggcaaactcg ccgacggcgg ctttgtgcgg atagactggg atgcggcgaa
    19021 agaagaagcc gtattgaagt ttaagaaaag caaggtcaaa ataaaaaccg cctccgcata
    19081 aaatcaaaaa tgccgtctga aaggttcaga cggcattttg tcaaatatcg gcccctattc
    19141 cggcaaactt ctccacaaac gccgcgattt tttggaagac gctttgtttc ttcgtcagat
    19201 attgcgggtt gagcggactc attttcggca gggtttcggt cagttccgtg ccgttttcgc
    19261 tggcatagcc gcgtttgagc gagccggtca aatagcgttt ggcggcggtt tcgttcagac
    19321 cttcggcggc aatcaatcct gccgcttcgt gccgcatcac ctcttgcgcg taggtgtaaa
    19381 aggtttccag tatggcggga acgtcgggca ctttatccaa atccgtatcg ttgatgaaat
    19441 ccacaatcag gccctctttg gcgcggtggc cgatgctggc gcggatgatg cggcggattt
    19501 cttccaccag ctccgctttg cctttgattt ttttgtgatg ctcgaaaacc agttgcagga
    19561 tgtaatccag attgatttcc tgcgatttga gcaaatccac ctcaaaaacc acatcgtccc
    19621 agtcgatttt tgactgttct ttctgttcgc ctgctttttg gcggcgcagc cagtctcgga
    19681 tgtcgttata ggcggaacgg tagtcctgca ccgccctttc agacggcatc ggcactttcc
    19741 gcatttcctg cacgtcttcg tcgctcaggt agtatttttc ctgaaacgcc ttcatcgcat
    19801 cttcgtccgc cgcgtccaca ttctgcaatt cgcgcagcgc ggcaaattca tcgtagttct
    19861 gcaatacgtt ttccgcccgc aggtattcgc cgaagagttt ggcaaaatct tttttgtctt
    19921 tttccgtttc gattttgtcg ggatcgggga aacgctcgtg caattctttt gctacatcca
    19981 gataaccgcg ccgtgcttcg ccggtctggc tgtcggtata gccgttcatg tattcttcgt
    20041 agcttttttc cagcactata tttttggtgt ttttgtcgcc gaagagcgtg atcgcatcaa
    20101 tggttgcctg ctccaagtcg cggaagcaga caatgttgcc gaaggttttg gtagcatcgt
    20161 aaatgcggtt ggtgcgagaa aacgcctgca tcaggccgtg atagcgcagg tttttatcga
    20221 cgaacagcgt attcagcgtc ggtgcgtcaa aacccgtcaa aaacatgccg accacaatca
    20281 gcaagtccac ttcccggttt ttcacccgtt ttgccaaatc gcggtagtag ttctgaaagg
    20341 ctttgctgtc cgtgccgaaa ttggttttga aacaggcgtt gtaatcgttg atggcagcct
    20401 gcaaaaattc tttcgcgctg ctgtccatcg cttccggttc aaaggtctcg tcaacaattt
    20461 caccgacggc gttttgctct tcgttggcgg caaaggaaaa aatggtggct gttttcaacg
    20521 ggtgcaggct gcctgcctgt tgtgttttga acgcttcgta atagcacttc gccgcatcca
    20581 cgctgctgac ggcaaacatg gcgttgaagc ctttgccgcc cgcattcaag cggtgcgttt
    20641 tctgcctgaa ccggctcagg atatattgcg tgatttcgcg gatgcgttcg gggtgcagca
    20701 gggcttggcg gttttcggcg gcactcagtt ccttctcgtc ctgttccgct tccacggctt
    20761 tgaactgcgg gcgcacatcg ttgtaatcca ctttgaattt caatactttt tcatcgcgga
    20821 tggcatcggt aatcacataa gaatgcagct cccgcccgaa cacgcccgcc gtggtttccg
    20881 cgcccaaagc gttttcggga aaaatcggcg tgccggtaaa gccgaactgg cagaattttt
    20941 taaatttctt tttcaggttt ttttgcgctt cgccgaattg cgagcggtgg cattcgtcga
    21001 aaataaatac aacctgccgg tgataaaccg gcagattatc ttcacttttc atcaggttgt
    21061 tcagcttttg gatggtggta acgatgattt tgttgccgtc tttttccaaa ttgcgtttca
    21121 agcccgccgt actttccgag ccgtttacgc tgtcgggcga aaaacgttgg tattccttca
    21181 tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa aaccttgtcg ataaacgccg
    21241 attccgtcgc cagacgcgcc gccttaaagc cggtcagcgt tttgccgctg cccgtggtat
    21301 gccaaacata gccgccgctt tccggcccgc tccaattctt cgcctgcgcc gagctgttga
    21361 ttttccacaa aatgcgttcg gcggcggcaa tctggtacgg ccgcataatc agcagcgtat
    21421 cattcgcatc gaacacgctg taatgcagca aaacgctcag caatacgctt ttctgcagga
    21481 acgtggcggt aaagtctttc aaatccttaa tcggatgatt gtccgaccgc gcccaattca
    21541 tcgtgaaatc gaagctgttt ttgtcgcgct tggtggtgtt ggcgaaataa cgcgtgtccg
    21601 tgccgttgga aatcacgaag atttgcagga atttgaacag cgaattttcg ctgttgaagc
    21661 tctctttgct gtaacggtgc acctgattga atgcctcgcg caccaccacg ccgcgctttt
    21721 tcaattcaat ctgcaccagc ggcaggccgt ttaccaacac ggtaacgtca taacggtttg
    21781 catgcgtgcc cgtctgctca aactggttga taagctgcac atggttgcgg gcaaggtttt
    21841 tcttgtccag cagataaatg tttttcagac ggccgtcatc aaaagcgaaa tcgtaaatat
    21901 ggtcgtcgtg gattttgcgg gttttatcgg taatgttttc agccggcctg tccagatatt
    21961 ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac atcgttcagc cgctgcaact
    22021 gcgcgcgcag gttttccaga agcctgcttt ggctgttcaa atccttgcgg tattcgtacc
    22081 cctgattctg caaatcggcg attaactccg cttccaaccg gttttccgat tggtagctgc
    22141 ccgactgttc gattttttca tattggtcga gcacgatgaa attcggcgtt tcggcgatgg
    22201 gtttggtttc gaggttcatg ggtcgtcctt tgcggtgtcg ggttttatag tgaattaaca
    22261 aaaaccggta ctgcgttggc tcgccttagc tcaaagagaa cgattcccta aggcgcccaa
    22321 gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggctcgccgc cttgtcctga
    22381 tttttgttaa ttcactataa acggtttgat gttcggattg ggctgcctga aaaagtgcag
    22441 gctgcttttt caagcagcct tggggaaggc aagcagctgt tcgcggtaat attcgtattg
    22501 tttgcggcgc agggcgattt cgtgcggcag gccttcgctg acggagtggg tcagggtatc
    22561 gaatttgcct aggatggcga cgattttttc ctgttcgggg agcgggggga tggggatttt
    22621 atattgcata atcgctgctt tgctgccacg cggcattttt gcacctttgg cgtgcttcat
    22681 attaaaagca aaaaatttat catcggccaa tacctgatac agatattttg gattaacctt
    22741 ttcatctgtt acccggataa ccaaaacatc accgtttgtt ccgcccgtgc agtctgcctg
    22801 ccagattttt ttcaaatacg gacggatatt tccaatcaaa atgtcattga caatatattc
    22861 tgtcattttt ccttcgcttg gaacataacc ggataacttt ttaccttctc tgttttgtaa
    22921 gagattatcc acgccaacgt aattatgttc gttcagttta tccgaacaaa tacggttttt
    22981 tgaatattcg gcaacctccc ccaacgtctt ccaaaccaca tttttcagac ggcattgata
    23041 gccatcagct atccccccga tttgattgtc aaaatctaaa agtaagtcgc ggtaataccg
    23101 gtattggcgt ttgcgcaggg ctaattccgc ttccagcgta gcttccagcg tagcttccag
    23161 ctcggtgaat ttgtcaagta tttttacaat tttttgttgg gtttccaatg atgggatggg
    23221 gattttatag ttatctgtat ctggtgttgc tatttgtggc atttgcattt ttgatccgat
    23281 attacgaaaa tagttttctt gtgtttttaa gaaataataa acatatttta tataaatatg
    23341 tttattaact gaatgataag accacatttc atttttatgc gaaaatggtt tatcgtaata
    23401 ttcgaattca ataatacctc ttgatttaac gataatagaa ggctctctat ggatatcctt
    23461 atcaggaaca tcatcaaagt caactaatgc aaaagttttt cctcctgcaa aaatctttaa
    23521 cggcgcatta tctttatgca tttctttcat ttgtccagca gttatcttag ttccttttgt
    23581 acgaactaaa acctccccca atggcttcca ctccaccggt gccgtctgaa tcatctcaat
    23641 caattttttc gctttgcttt gcatatccat ggttgttttc cgtttccttc ccgctgcggc
    23701 gggatgtggg gcggttgcgg ggcgggtttc ggctcactct gtccgtttgc cgttgtttct
    23761 gcgggctgaa acccgtcctg ctttcaggct gcctgaaatc gtgtgcaggc tgctttttca
    23821 ggtagcctga atgcggttat tttttgccgt tttgatgctg ttcggttgcg gcaagccttt
    23881 tctgttcttc gttctcaagc tgtttgatgc ttttctcggg tacgggctgg ttttcaggca
    23941 tgatgccgcc cagttcttca atggtttggc gcactttttg tccgacttcg aaatgcaccc
    24001 ggtttgcctg tgttttgcct tggatgtttt tgcggcgtag tttttcttcg gtttgcgtgg
    24061 cgcgaaacag attggcagcc ggttcgctgg cgttcatgtg gtctaggatt ctctggcttt
    24121 tcttcaagcc tttgcggctg tgtatgccct gcttgtccag tcctccgtat agcccccgat
    24181 agccgtggtt ttggaagacg gcgtactcaa cgggtttttc tacgcctgca tccttggctg
    24241 cggcggcaag gtcggtattg tgttcgcgca gctgcctgcg aagcagcagg cgttgtttgt
    24301 cttcgcccaa actcctgaat gctgcctcgt cttgcagctc ttggcggcgg gcttgcacgg
    24361 caaagtaggt ttgccctgcg gcaatgacgc ttttggacgg atcgccgttt tgcacaatca
    24421 ggtagcaggc atagcgggaa aggcggtaat ccgccacttc gcgctgcccg cctttcccaa
    24481 gggctatcat tttgttggtt tcaacaaaat gatgattttt gtcatttcct gatgtttcgc
    24541 aagcggttat ggctttatca atggcccgtt ggaaattgcg ccattcggaa tattccaaga
    24601 tttgctgcaa ggtgcgcgcc gaccaatatt cattgcctgt ttcgtcgatg tgtttggcgt
    24661 tttcaaaagc gttattttct gttgtcatgt attttccccg aaaatttctt gatttgaact
    24721 tgtaaggatt gcttacaggt tgccgcccgt attttcagac ggcctctttc ccattcagtt
    24781 gttgcaaaat atgcaacaac tgcctaggtt tcaatctctg caatcacttc gtcaatttca
    24841 cgccgcagcc gttcgatttt ggcgacggtt tcgctgattt cggcgttgag ctgtctgatg
    24901 tcgatgacct cgcgggtgtc ttcggcttcg acatagctgc tgacggcgag gttgtagccg
    24961 ttgtctttga cggtttgctg ggcggcgttt tgggcgatat gcggcacatc ggctttgtcg
    25021 gcgaagagtt tgacgatttc ggcaatgtgt tcttcggtta agacgttgtt gttggtttct
    25081 tttttaaaga agccgcttgc gtcgatgaat tggatgtcgg tattgtcttt gtgtttggac
    25141 aaaaccagga tattgacggc gatgcaggtg ccgtaaaaga gattgggcgc aagggcaatc
    25201 acggtttcca catagttgcc ctccaccaga tattggcgga ttttctgctc tgcgccgccg
    25261 cgatagaaaa tgccggggaa tgagacgata gcggcgcggc ctctgccgga aaggtagttc
    25321 agtgcgtgca ggatgaaggc aaaatcggct ttggatttcg gtgcgagtac gcctgcgggg
    25381 gcaaagcggt cgtcgttgat caaggtgggg tcgtcgctgc ctatccagtc gatggaatag
    25441 ggcggattgg agacgacggc atcaaagggt ttgctgtctt tgagtttggg gttggtcagc
    25501 gtgtcgccca attcgatgtg gaatttgttg taattgacgt tgtgcagaaa catattcatg
    25561 cgggcgaggt tgtaggtggt gtggttgatt tcctgcccga agaagccttc ttcgatgatg
    25621 tgttcgtcaa actgtttttt cgcctgcaag agcaggctgc ccgagccgca ggcggggtcg
    25681 tagattttgt tgactttctc ctgcccgtgc accgccagcc gcgcaatcag cttggagacg
    25741 ctttgcgggg tgaaaaattc gccgccggat ttgcctgcgt tggcggcgta gttggaaatc
    25801 aggtattcgt aggcatcacc gaaaaggtcg atgcggtggt cttcaaaatt gccgaaatcg
    25861 agttccgcca cgcctttaag gacggcggca aggcgtttgt ttttgtcggc aacggtgctg
    25921 ccgagccggc tgctggtggg tcgaagtcgt caaacaagcc tttgatgcct tgttcggacg
    25981 ggtagccgga ggcggagctt tcaatcgcgg taaagatttc tttcagcttg gtgttgagct
    26041 cttcgttttg atgggcttcg gcggcaatat tgcaaaaaag ctggccgggg tagatgaaat
    26101 agcctttgac tttgacggca tcgtctttga tttcgggcgt gatgatgctg tccggcatag
    26161 cggcgtaatc aatgctgctg tcgccggcct gcatatagtc ggtgaagttt tcgctgataa
    26221 agcggtagaa aagtgtgccg agaacgtatt gtttaaagtc ccagccatcc accgcgccgc
    26281 gtacttcgtc ggcgattttc caaatttggc ggtgcagttg ggcgcgttgt tgcatttcgg
    26341 tcatcatcga aatccatata taaagttaaa caaatcaaaa tcgcctgata ttttcagacg
    26401 atttttttac gggcattcaa attaagccaa attttccagc atccatttga cgtagcggtt
    26461 gacacctgct ttgacatcga aaaattcttc cttatatccg gcttcgcgca atttggtgat
    26521 gtcggcttgg gtgaagccct ggtatttgcc tttgagcgcg tcgggaaagg ggatatagcg
    26581 gataagttct tcttctacca actctttcaa gctcaattca gatttgcctt cggcggcgcg
    26641 gcaggcgttg acggcggcgg cggcgagttc gttgaactgt tggctgcggc cggtgccgag
    26701 gttgtagatg ccggaaagtt cgggatggtc gaagaagtag aggttgattt tggcgacgtc
    26761 ttcgacgctg acgaagtcgc gggtttgttc gccgttgccg tagccgtcgt tgctgccgaa
    26821 caggttgacg taaccgtgtt cgcggtattg gtggaaatgg tggaaggcga cggatgccat
    26881 gcggcctttg tgttgttcgt gttgtccgta aacgttgaag tagcggaagc cgacgacttg
    26941 ggcggtgaga ccttctttca tgcggcggcg caatacttgg tcaaacagga atttggagta
    27001 gccgtacaca ttaagcggtt tttcgagttc gcgctcttcg cggaagattt cgcctttgcc
    27061 gtaaaccgcc gcactggagg cataaaggaa ggggatgcgt tcgtcctgac accagtccaa
    27121 caaatccagc gtgtactggt agttgttttc catcatatac aaaccgtcgt ggttcatcgt
    27181 atcggaacac gcgccttgat ggaaaacggc ttcgatgttt tgataaggta aaatgtgttc
    27241 cctcacttgg cggataaatt cgtgtttgtc gaggtagtgg gcgatttcgc actcggcaag
    27301 gtttttgaat ttttcgcctt ttgtcagatt atcgacggcg acgatgtcgg taatgccgcg
    27361 ttggttgagg gctttgacga tgttgctgcc gataaagccg gccgcgcctg ttacgatgat
    27421 ggtcatattc ggtttccttt gttttgaggg tttcagacgg cattggatgg tcatgccgtc
    27481 tgaaaagatt tattgcccgt ccagtgcttc aaccaactct gcaaacgagc aaaccgccgt
    27541 accgagtttc gccacgacaa ccccggccgc agtattggca aggtacatgg cttcgggcat
    27601 ggtgcagcct gccgccagcc ccaagcccat tccggcaatg acggtgtcgc ctgcgccgga
    27661 cacgtcgtaa acttcttggg cgcgggtggg ctggtaaatg ggttcgcctt cgctgaacaa
    27721 ggtcatgccc tcttcgctcc gggtcagcaa aacggcggtc aagtcgaggt ggcggcgcag
    27781 gttttgcgct ttttcggtca gatcgccttc gtttttccaa ctgccgacca cttctttcaa
    27841 ttcggcgcag ttgggcgtaa tcagagttgc gccgacatat ttttcgtaat cgtcgccttt
    27901 ggggtcgatt aagacggttt tgccgacatg tttcgcccaa tcgatcatat cggagatatg
    27961 cgacagaccg cctttgccgt agtccgaaaa aatgattgcg tcgtattcgg gcaagatttc
    28021 acggtatttc cgcttgattt gttccaacac ttcgcggttg ggatgttctt caaaatcaag
    28081 gcggataagc tgctggttgc gggcgacgac gcgcagtttg acggtggtgg cgatttgttt
    28141 gtcgcgcatc agataggagg cgacgccgtc ctgcaccatc agcgcgtcga gcgcggcggc
    28201 ggcttcgtcg tcgccggtta cggacaacag ccctgccctg ccgcccaacg aagcgatgtt
    28261 gcgcgcaacg ttcgccgcgc cgcccgcccg ttggtcgatt cgtccgattt ttgccaccgg
    28321 cacgggtgct tcgggcgaaa ttcgggacac gtcgccaaac caatagcggt cgagcatcac
    28381 gtcgccgaca acaagaactt tggcttgtgc gaaacgggat ttgagggttt cttgttggaa
    28441 cttggcgggc atattgcctc cgttgtgttt tgttcagaca accttaacct gtaagaccgc
    28501 ctgaaaatca gtttgcttca aggtttgcca cgcggttcac ttcgcgcaat acggcgaccg
    28561 gatcggcggc tcgggtaacg gggcggccca tcaccagata agtcgaaccc gccgccaagg
    28621 cttcggcggg tgtcataatg cggcgttggt cgtcgttgtt gccggcaacg tccaagcgga
    28681 tacccggtgt aaccaagaca aaatcccgtc ccaattcgcg gcgcagcggc gcggcttctt
    28741 gggcggaaca gaccacgccg tccaagcccg aactttgtgc cagttttgcc aaacggatga
    28801 cttgttcttc aggggcggtg ttcaaaccga tttccgccaa atcgctttgc tccatgctgg
    28861 tcaacacggt tacgccgatt aagagcggtt tcgtgccgta tccggcaacg gcttcggcgg
    28921 cggcttccat catacgtctg ccgcccgacg cgtgcatatc gaccatccac acgcccatat
    28981 cggcggcgac tttacacgcc tgcgcgacgg tgtggggaat atcgtggtat ttcaaatcaa
    29041 gaaagagttt gaaaccctga tgaatcaggc tttctgccaa actgcgcccc gtcgcggtaa
    29101 acagctcttt gccgattttg atttggcaca acgccggatc aaggttgcgg acgaatccga
    29161 gcgtgtcttt ttcgttggca aaatcaaggg caacgatgac gggggtacgt tgttgcggag
    29221 tttggaagtc ggtgattaaa ggattcatgg tgttttcgtt tcgggttttc agacaacctt
    29281 tatcgtcttt ttcgcagtat agcggattaa caaaaaccgg tacgtcgttg ccccgccccg
    29341 gctcaaaggg aacgattccc taaggcgccc aagcaccagg cgaaccggtt ccgtactatc
    29401 cgtactgtct gcggcccgcc gccttgtcct gatttttgtt aatccgctat aacaacgtaa
    29461 attcaaaccg taggccgggc attgatgtcc gacctacgcg gactcaagcc cccgacaaac
    29521 tgtttcggat aaatttgaac cggtacatca gccgtcattc ccgcgcgggc gggaatccat
    29581 tgataaaact cagtttatca gatttaaaca gcgattgccc aaattcagaa atggattccc
    29641 gcctgcgcgg gaatgacgcc gaatgattat ttttcggttg caacacgctg tttatcaaaa
    29701 acaggtcgtc tgaaaacctg attttctgtt ttcagagtgc gaagcctgcc cgtgggtacg
    29761 accttttcga tttatttgcc caatgcgccc aatacttcgg ctttcacagc ttctactgct
    29821 tgggtaccgt cgactttgat gtatttcggc gcgtgttcgc cttccagctt gctgtaaaaa
    29881 tcaaccaaaa cttcggtttg ctcgtggtaa acggcaagac gttttttcac ggtttcttct
    29941 ttgtcgtcgt cgcgctgaat caaatcttcg ccggttacgt cgtctttgcc ttcggtttta
    30001 ggcggattgt aggtaacgtg gtaagtacgg ccggaagcca aatgcacgcg gcggccgctc
    30061 atgcggtcga caatcacgct gtcggatacg tcgatttcaa cgaccgcgtc caaacccacg
    30121 cctgcttcca ccatcgcttc ggcttgcgcc aatgtacgcg ggaaaccgtc gaacaggaaa
    30181 ccgtttttgc agtcgtcttg cgcgatgcgt tctttgacca tgccgataat gatgtcgtcg
    30241 cgcaccaaac cgccttcatc gatgattttt ttcgcttcca aacccaaggg cgtgcctgcc
    30301 ttaatcgcgg cacggagcat gtcgccggta gagatttgcg gaatgccgaa cgcggcggtg
    30361 atgaattgcg cctgagtgcc tttgcccgcg cccggtgcgc ctaaaagtaa tgctttcatg
    30421 aagtgatcct tttaaatgtg tttattggaa tcaaacagat ggtttacgat tctacacggc
    30481 ttggcgcaat agttcaaaaa ccaactgcac aaactgccat ttgcaagcaa atgttttgca
    30541 tacctttgcc gagcctgata ttcacaaggc ggcaactttt tgggacaatc cccaaagttt
    30601 caaaacatca caccctacca aaaaggaaca tccgtgaaac gcatttttct gtttttggct
    30661 accaatatcg ctgttttggt cgtaatcaac attgttttgg cggttctggg catcaacagc
    30721 cggggcggcg cgggcagcct gttggcgtat tccgccgtcg tcggcttcac tggttcgatt
    30781 atttcgctgc tgatgtccaa atttatcgcc aaacaatcgg tcggtgcgga agtcatcgac
    30841 acgccgcgca ccgaagaaga agcctggctt ctgaacactg tcgaagccca agcgcggcaa
    30901 tggaacctga aaacgccaga agtcgccatc taccactccc ccgaacccaa tgcctttgcc
    30961 acgggcgcat cgagaaacag ctccctgatc gccgtcagca ccggtttgct cgaccatatg
    31021 acgcgcgacg aagtggaagc cgtgttggcg cacgaaatgg cgcacgtcgg caacggcgac
    31081 atggttacgc tgacgctgat tcaaggcgtg gtcaatacct ttgtcgtgtt cctgtcgcgc
    31141 attattgcca acctgattgc ccgaaacaac gacggcagcc agtcccaggg aacttatttc
    31201 ctagtcagca tggtattcca aatcctgttc ggcttccttg ccagcctgat tgtcatgtgg
    31261 ttcagccgcc aacgcgaata ccgcgccgac gcgggcgcgg caaaactggt cggcgcaccg
    31321 aaaatgattt ccgccctgca aaggcttaaa ggcaacccgg tcgatttgcc cgaagaaatg
    31381 aacgcaatgg gcatcgccgg agatacgcgc gactccctgc tcagcaccca cccttcgctg
    31441 gacaaccgaa tcgcccgcct caaatcgctt taaaccgatt tgaaacgaca aaaaaccgta
    31501 cgcacaagca gtacggtttt ttcgtatatg ccgactgaaa acatcaggtt tccataccgg
    31561 caccaaccgt atccatttcc gcaccggata cgccgcattg atatatcccc gacaaaccgt
    31621 gtccggacag aaaatatcag ataaaacagc ggtaaaaatg ccgtctgaaa ccaattaggg
    31681 cttcagacgg catttttact gcaaaacctt atgcgaacgg atgatgcagc acaatcgttt
    31741 cttcgcggtc ggggccggtg gagacgatgg cgaccggcgc gccgcagact tcttcaatcc
    31801 gtttcaaata tgctttggca ttggcaggca atgcgtcgta gcttttcacg ccgacagtcg
    31861 attcgcgcca gccgggcatg gtttcgtaaa tcggtttgca ggtttccacc gcatccgaac
    31921 cgcaaggcag gatgtcggtt ttgccgccgc ctggcaattc gtagccgacg cagatattga
    31981 tggtttcaac gccgtccatt acatcgagtt tggtaatgca cataccggaa atgccgttga
    32041 tttggatgga gcgtttcagg gcggcggcat caaaccagcc gcagcggcgc gcgcggccgg
    32101 ttaccgaacc gaattcgtgt ccgcgctccg ccaaacctgc gcctacttcg tcgaacaatt
    32161 cggtcgggaa cgggcccgaa ccgacgcgcg tggtataggc tttgacgatg cccaaaacat
    32221 aatccagcat ttgaggacct acgcccgcgc ctgccgaagc cgcgccggcg agacagttgg
    32281 acgaggtaac gaaggggtaa gtgccgtagt cgatgtccaa caacgcacct tgcgcgcctt
    32341 caaacagcag tttttcgccg tttttgtttt tttcgttcaa cacgcgggac acgtcggcaa
    32401 tcatcggcgc aatgcgcggc gcgacttttt cgataaccgc catcacgtct tccgctttaa
    32461 ccggtccggc gttgtgcagg tattggagtt ggacgttgta ataggcaagg acggcatcca
    32521 gtttttcacg cagtttttca ggatgcagca aatcggcggc gcgaatggcg cggcgtgcca
    32581 ctttgtcttc gtaggcaggg ccgatgccgc ggccggtcgt gccgattttg cctttgccgc
    32641 gcgatgcttc gcgggcttgg tcgagcgcga tgtggtaagg caggatcagc gggcaggtcg
    32701 gcgcgatttt cagacggcct tcgacgtttt tcacgcctgc cgcgttcaac tcgtcaattt
    32761 cgcccaacag ggcttcgggg gaaacgacaa cgcccgaacc gatgaagcaa tccaatcctt
    32821 cgtgcaggat accgctcgga atcaggcgca aaatggtttt tttgccgccg acgaccaagg
    32881 tatggcccgc attgtggccg ccttggaagc gcacgacgcc gccggcttct tccgccagcc
    32941 agtcaacgat tttaccttta ccctcgtcgc cccactgtgc gccgattact acaacatttt
    33001 tagccatagc catataacct atcgatatta aaaattattg cggaaaatcc ccaaaccggt
    33061 gcggctttgc gccccgcccc ggtttgcctt tcatccgcta tgaacgggta tttagcgttt
    33121 cacgacctgc caaacgccgt ccgtcttttt cagacggcct gcaagctctt ccgaaacatt
    33181 gtgtccgata ccgtagtcaa tcacgacaca ctgcccttgt tcacgcaagg cttcgaccgc
    33241 ttcgcgcgcc gcttctgcat cttccgcatc gaccaacacg gcgggctgcc gttcgacggc
    33301 gggcaaacgc ccgataaagc tgcgtaagtc gaaactgaat cccgttgccg ggcgcgccct
    33361 gccgaaatat ccgcccaatc cgtcataacg cccgccgcgc gcgaccgcgt cgtggaaatc
    33421 ggcggcatag gcggcataca gcaagcccgt gtggtaattg tcgacgcgca gctcggacaa
    33481 gtcgatatgg atttcattat cggggaatgc gtcgcacacc gcctgcaatt cgtccaacgc
    33541 gccgccgacc gccgacaaat ccggcaaccg tccgcgcgcg tcggacaaca cttcacgccc
    33601 gccgtacagg cgcggcagca acgagaatgc ttttgcccac ataccgtcca gcttccaagc
    33661 cttgacctgc gcttcgaccg acccggtatc tttatcctgc atcaaggcaa gcagcgctgc
    33721 ggactgcccc gcatccaaat gcgccgcatc ggacaaggcg cggaatatgc cgatatgccc
    33781 cagcgaaagc agcactttgc ccatatcggc aattttcatg cttttcagca taaggtctat
    33841 cagctcgatg tcgccacgga tgtcggcaaa accgtacatt tccgcccctg cctgcaaggg
    33901 ttcgcgcata ttcggcagac cgtcgggctg cgcgtgcaac accggaccgg cataacacaa
    33961 ccggttaatc ccttggttgg cggacaaaag atgggcatcg atacgcgcca cctgcggcgt
    34021 gatgtcggcg cgtatgccca actgcctgcc gctgagcctg tccgttacca aaatggtttt
    34081 cagtgaaagc cccgcatcga tatgcgtcag cagggaatgt gcgtactcca tcagcggagg
    34141 ctgtaccagt tcataaccgt gtacgcggaa cagtgccaac aactgctcgc gcgcgctttc
    34201 aagctgccgc gcgttcgtgg gcagtacgtc ggcgatatgt tccggaagct gccatgtctg
    34261 catgagaatc tgccttctgt tttatcctgt aaacacaaaa gactgcccga tccgcaatca
    34321 gacggcatct ttgccgcaac gcatcacgcc gctgtcaaaa cggcgatgct tccttccgat
    34381 tcagaatccg aacagcccct gtccgacggc tcaatgccgt caagtcgtta aaaccaaact
    34441 ttaccataaa atacacacaa tctgaatata cttttatacc ccgtatccgc cgtctgacgg
    34501 cacggtattt tttactatat aatgtgcacc attatttctc atcacttccc gctgccgttt
    34561 ttttgtttca gacggcattt ttattgactg aacacttatg ctgctcgacc tcgaccgctt
    34621 ttccttttcc gtcttcctga aagaaatccg cctgctgacc gcccttgccc tgcccatgct
    34681 gttggcgcag gtcgcgcagg tgggcatcgg tttcgtcgat accgtgatgg cgggcggtgc
    34741 gggcaaggaa gatttggcgg cggtggcttt gggcagcagc gcgtttgcca cggtttatat
    34801 tacctttatg ggcattatgg cggcgctgaa cccgatgatt gcccagcttt acggcgcggg
    34861 taaaaccggt gaagcaggcg aaacggggcg gcaggggatt tggttcgggc tgattttggg
    34921 gattttcggc atgattttga tgtgggcggc gattacgccg ttccgcaact ggctgacttt
    34981 gagcgattat gtggaaggca caatggcgca gtatatgctg ttcaccagct tggcgatgcc
    35041 ggcggcaatg gtacaccgcg cactgcacgc ctacgcttcc agcctgaacc gcccgcgcct
    35101 gattatgttg gtcagctttg cggcgtttgt gttgaacgtg ccgctgaact atattttcgt
    35161 ttacggcaaa ttcggtatgc ccgctttggg tggcgcaggt tgcggcgtgg cgacaatggc
    35221 ggtgttttgg ttcagcgcgc tggcattgtg gatttatatc gccaaggaaa aattcttccg
    35281 cccgttcgga ctgacagcga aattcggcaa accggattgg gcggtgttca aacagatttg
    35341 gaaaatcggc gcgcccatcg ggctgtctta ttttttggaa gccagcgcgt tttcgtttat
    35401 cgtgtttttg attgcgcctt tcggcgagga ttatgtggcg gcgcagcagg tcggcatcag
    35461 tttgtcgggg attctctata tgattccgca aagcgtcggc tcggcaggga cggtgcgcat
    35521 cggcttttcg cttgggcggc gcgaattttc gcgggcgcgt tatatttcag gagtatcgct
    35581 ggtgtcgggc tgggtgctcg ccgtgattac cgtgctttcc ttggtattat tccgttcgcc
    35641 gctggcaagc atgtacaacg atgatccggc agttttaagc attgcctcca ccgtcctgct
    35701 gttcgccggc ctgttccaac cggcagactt tacccaatgt atcgcgtcct atgccctgcg
    35761 cggctacaaa gtcaccaagg tgccgatgtt catccacgcc gccgccttct ggggctgcgg
    35821 cctgctgccg ggctatctgc tcgcctaccg tttcgatatg ggcatttacg gcttctggac
    35881 ggcattgatt gcctcgctca ccatcgcagc cgtcgccttg gtgtggtgct tggaaaaata
    35941 cagtatggag ttggtcaaat cacacgaggc cgtctgaaaa cgcagcacac ttcaattcaa
    36001 atacagttaa agctcaaacc atgcaaccca tccgataccg aaccgacctc accccctaca
    36061 acactttcgg cctgcacgcc caagcccggg cctttatcgc gcttaaacac gccgacgaat
    36121 tgcgcgacat cgtccgactg cccgagttcg accgcgatac tgttttatgg ctgggcggcg
    36181 gcagcaacat ccttttgatg caggattacg acggcttggt cgtccatatg gaaaacaagg
    36241 gcatacgcga gattgcgcgc tcagacggca tggttctgat tgaagcgcag gcgggcgaaa
    36301 tttggcacga tttcgtgttg cacaccgtcg cgctgggttt gagcggtttg gaaaacctga
    36361 gcctgattcc gggtacggtc ggcgcgtcgc ccgtgcagaa catcggcgca tacggcgtgg
    36421 aggcgaaaga tgtgattcac agcgtgcgct gcttcgattt ggatacggaa acctttgtaa
    36481 ccctttccaa tgccgactgc cgcttcgcct accgcgaaag cctgttcaag caggaaggca
    36541 aagggcgtta tgtgattgtt tcggtcgtat ttgcattaaa aacgcatttt gtgccgaact
    36601 tgggctacgg cgatttggcg gcgaaggttg ccgaattgag tgcaggtagg gaggcgacgg
    36661 cgaaagacgt ttccgatgcg gtatccgcca tccgcaacag caaattaccc gacccgaaag
    36721 ttttgggcaa tgtcggcagt ttctttaaaa accccgtcgt cagcgcggaa aaagccgcca
    36781 ccctgttgca gcggcatccc gatatgccgc gctatccgca gcccgacggt tcggtcaaac
    36841 tcgccgccgg ctggctgatc gaccaatgcc gtctgaaggg cttccaaatc ggcggtgcgg
    36901 cggtacatga ccggcaggct ctggttttgg cgaacaaaaa caatgcctct tcagacgaca
    36961 tctggaaact ggcgcaacac gtctgcaata cagtatttac tcgatttcag gtagaattac
    37021 acgccgaacc caattggctg cccgcttcgt tcagcctgta aatccaagga gtatgcccgt
    37081 gacccgcatt gcaaaaacca atacttacac ccgcatcatc gacgccagcc ttgcgctttt
    37141 caacgaggaa ggcgagcgca acatcagcac caaccatatt gccgcccact tgggcatcag
    37201 tccgggcaat ctctattacc acttccgcaa caaagacgaa atcatcgtcc aactgttcaa
    37261 acgttacagc gaagccctgc tggaatacct caacgaagcc gtgttgccct ctgatgtgga
    37321 agactctatc aactatatgg caggcattta cgatgtcatg tgggaatacc gcttcctctt
    37381 tagcgacgta aacaccctac ttgcgcgcag tgccgaattg ttgggcgaac acaatacctt
    37441 tacccaagcc aaagtctcgc cgcttctagt caaactgctg acccagctca acgggctgaa
    37501 cgtcatcaac gccgaccaaa ccgctatgaa cgacctcgcc gtcaatatgt ggatggttac
    37561 gaaatactgg ttcgacttcg acagttccct gcgcggccgt accaagctga ccgaagactc
    37621 caaagcacgc ggcgtccgtc gtaccttaag cctccttcgc ccctatcttt tgcccgaaca
    37681 ccgcgcggaa tacgaccgca aaatcagcaa cggcaacccg taaacccata acgtccgaat
    37741 cagccttcag acggcactta cccgacggta tcagcgaatg cgcgcagtcc ccccgcccca
    37801 tatctccatc acaggcctag gttacctcgg cctgccgctg gcacaaaagt tttaccgaca
    37861 cggcagccgc gtcgccgcca taaaacgcag cctgacttcg gacgacatca acctgcccat
    37921 acacctcgac accttcgacc tcaaccgtac cgataccttt cagacggcat tgtggcggca
    37981 tcacgccgac aaacccgtct ggttcttcct gctgccgccc tcctttttgg cgcattacgc
    38041 cgacaccgtc aaacaatggg cagagcttgc ccgggcgtgc aacgtgcaac acctgatttt
    38101 cacaagcagc acaagcgttt acggcgataa agcgcgagaa tgcaacgaaa ccgccgcacc
    38161 cgatccgcaa accgagtccg cccgacaaat ccttgccgcc gaacaacacc tgctcgacaa
    38221 cggcgttccg aacatcgaca tcctgcggct gggcgggctt tattgcgccg aacgccatcc
    38281 cgtcagccgc ctcgtgcaaa agcaaaacat ccaaggcggc aaccggcctg tcaatatcgt
    38341 ccaccgcgac atcgccgtcg aaaacctgtt tcagacggca tccaaccccg gcggcaggcg
    38401 gctgaaaaac attatcgaac cgcgccaccc gacacgccgc gaattttata cggaagaagc
    38461 cgccaaactc ggcctgcccg cgccggattt ctcacccgac gacagcgtgg gcaaaatcat
    38521 ccgtaccgtt tgcgataacg gcttaagcct gtaaaataag cggcaacagc aacaacaggc
    38581 attctccgcc ccaatcagaa aatatgtccg ccctcctccc catcatcaac cgcctgattc
    38641 tgcaaagccc ggacagccgc tcggaactta cctcctttgc aggcaaaaca ctgaccctga
    38701 acattgccgg gctgaaactg gcgggacgca tcacagaaga cggtttgctc tcggcgggaa
    38761 acggctttgc agacaccgaa attaccttcc gcaacagcgc gatacggaaa atcctccaag
    38821 gcggcgaacc cggggctggc gacatcaggc tcgaaggcga cctcatcctc ggcatcgcgg
    38881 tactgtccct gctcggcagc ctgcgttccc gcgcatcgga cgaattggca cggattttcg
    38941 gcacgcaggc aggcatcggc agccgtgcca ccgacatcgg acacggcatc aaacaaatcg
    39001 gcaggaacat cgccgaacaa atcggcggat tttcccgcga acccgagtcc gcaaacaccg
    39061 gcaacgaagc ccttgccgac tgcctcgacg aaataagcag actgcgcgac ggcgtggaac
    39121 gcctcaacga acgcctcgac aggctcgaac gcgacatttg gatagactaa ccttcagacg
    39181 gcatccgaca tgaacagccc ttttcacaac atcggtatcg taacccgccc caacacgccc
    39241 gacatccaag ataccgcaca cacgctgatt acctttttga agcagcacgg ctttaccgtc
    39301 tatctcgacg aagtcggcgt aagggaatgc tgcatctata cccaagacac cgacggctgc
    39361 catatcgtca acaagaccga actggggcaa tactgcgacc tggtcgccgt cttaggcgga
    39421 gacggcacct ttctctctgc cgcccgcgaa atcacccccc gcgccgttcc gattatcggc
    39481 atcaaccaag gtcatttggg ctttcttacc caaattcccc gcgaatatat gacggacaag
    39541 ctattgcccg ttttagaagg aaaatacctt gccgaagagc gcatcctgat tgaagccgca
    39601 ctgatccgcg aaggcaaaac cgccgaacgc gccctcgccc tcaacgatgc cgtcctctcc
    39661 cgcggcggtg ccggacagat gattgaattt gaagtcttcg tcaatcagga attcgtctat
    39721 acacaacgtt cggacgggct gattgtctcc acccccaccg gatcgaccgc ctattcgctt
    39781 gccgccggcg gcccaatcat gcaggcagga ttacacgcct tcacgctcgt ccccatctgc
    39841 ccgcaatcta tgactaaccg ccccatcgcc attccagaca cgtccgaaat cgaaatcctc
    39901 gttacccaag gcggcgacgc gcgcgttcat ttcgacggac aatccttcat cgacgtgcaa
    39961 aacctcgacc gcatcatcat ccgccgctac cacaatccct tacgcatcct acacccgacc
    40021 gactaccaat atttcaaaac cctgcgccaa aaactgcact ggggcgagca attagtctaa
    40081 gccggccttc acccgaaagt acccatgaat tccaaaattt cctccgacca tacagaagat
    40141 accatacgcc tctccaaacg tatggcacaa ctggggcttt gttcgcgccg cgaagccgac
    40201 ggccatatcg aacagggctg ggtaacggtc aacggcaaaa ccgccgtact cggtcagaaa
    40261 gtttcaccgg cagaccgtat cgaactgaac aagaaagccc acgaacagca ggcggcacgc
    40321 attaccatcc tgctgaacaa acccgtcggc tatgtcagcg cacaagcgga aaaaggctat
    40381 aaatccgccg ccgaactgat tacccctgaa aattgctggg aaggcgacac cagccgcatc
    40441 agtttcgatc cgaaacacaa aatcggtctt gcccccgccg gcaggctcga tatcgattca
    40501 gtcggattgc tggtgttgac gcaagacggc cgtatcgcca agcagcttat cggcgaaaac
    40561 agcggcagtg aaaaagaata tttggtgcgc gtgcgcggca aattggacga aaaaggactt
    40621 gccttactga atcacggatt gagtttggac ggtgataaac tgcgtcccgc caaagtagaa
    40681 tggcaaaatg aagaccaact gcgcttcgtg ttgaaacagg gtaaaaagcg gcaaatccgc
    40741 cgtatgtgcg aactggtcgg actgcgcgtc gtcgggctga aacgcatccg catgggcaag
    40801 gtcaaactcg gcaggctgcc gcccggcaaa tggcgttatc tcgctcccgg cgaatcgttt
    40861 taaataagca ttaaaaaatg ccgtctgaaa atccttgcgt tttcagacgg cactgcctta
    40921 tgcccaaata acggtttctt ccaagggcct gcgcgctttc gtgacgattt cctgaacccg
    40981 gtagccgaat atcgcggcaa cggacacgcc ccattctgcc gcatcgaaca aaccgaactg
    41041 cccggacaat acgcgttcca tatcggcata gttgaaacct tccaccgggc aggaatcgat
    41101 acccgccatc gccgcacccg tcatcatgtt ggctaacgcg atataggtgt ggcggcagca
    41161 ccagtcaaac aaggcgcgcg aatcgtccaa aatcttgatg tcgtcagctt gaaacgcctg
    41221 atacctcgcc aaagattttt ctacggcatc cggttcggta acgccgcgcc gtttgagact
    41281 ttccaacata aacgggctgt cgaagcgggc atttttcttc gccaaaaaca ccaccaaatg
    41341 actggcggta tccaaagcat ccgccatacc ccaagaaaac agcttgattg cctgtcggat
    41401 ttcggggttt tgaaccacaa caaaccgcca aggctccgaa ccgaccgaac tgggcgacaa
    41461 acgcccgagt tctaaaataa actgaaaatc ctcggcactg attttacgtg cgcatcgtaa
    41521 tgccggcacg atttgcggtt tttaaatgcg gatagaacct gctctttgct taatactgtc
    41581 atcgtgcatc cttttattac tttgattgca aattgatttc aaaccgatac ggcactgccg
    41641 caccctataa gccgtccccg ccatcaagct tcacccggat tttacccatc tctctacggc
    41701 tgggaaaaca aagcggcggc agattcggga ataactcccc aatgcctttc agacggcatt
    41761 tggacatcgc aaacgcgcct acgggtaggc gatataggca taagccgaat ggttgtggat
    41821 agactcgaaa ttctcgctct cgacgacgaa actcttgatg cgtttgtcgg caatcagcga
    41881 agtagcgaca tcgcgcacca tatcttccac gaatttcggg ttttcgtagg ccttttcggt
    41941 aacgtatttt tcatcggggc gtttgagcag gccgtagagt tggcagctcg cctgcgtttc
    42001 cacataatcg ataacttcct cgataccgac ttcggcatcg gaagtcaggc tgacggtaac
    42061 gtgcgaacgc tgattatgcg cgccatattg ggaaatttct ttggaacacg ggcaaagcga
    42121 ggttacggga atcatgacct tcatactgtg gccgtatgcc ccgtctttca tttcacccgt
    42181 gaggctgaca tcataatcca gtaaggaccg gataccggat accggcgcgg ttttcttgcg
    42241 gaaaaacgga aaagaaacgc tgattttgcc ggcgcgggaa tccaaaagcg cgaccatttc
    42301 ggcagtcagc ctatgcaatt gtgcgaaatc caagacttcg gtatgttgct ccatcaatgc
    42361 gacaaagcgc gacatatgcg tccctttctg ctcggcgggc agataaaccg tcatcgtcag
    42421 gcgagcaacc gtggattgtg ttccttcggc ggttttcagg gtaatcggga agcgcaggtc
    42481 tttaatgccg acctgattga tcggcaggtt gcgcaaatct ctgctggatt gcacgtctgc
    42541 aatggcgttc atgcgttgtt tgtccttaat attcagataa ttattgagat gtgtgtattg
    42601 tatggcaggc agatgccgtc tgaaaaaacg ctgtcggccg cctgcctgca aatgcgagat
    42661 tatatcactt gcttttggcg gctgcattga ttgtttctat tttcgggata gtgataaaat
    42721 cccgacttat ccatatctta caaggcagaa acagatgaaa ttcccgaccc gtgccatcca
    42781 ttccggctac gattgcgacg aacacaaccg cgcgctgatg ccgccgattt atcaaaacag
    42841 tatgtttgcg ctgcacgaaa tcggcgaaaa tgtgccttac cgttattcgc gcctgagcaa
    42901 cccgacccgt cagattttgg aagacaccgt tgccgatttg gaacacggtg cggcaggttt
    42961 tgcgttttcc agcggtatgg cggggattga tgccgtgtgg cgtaccttcc tgcgcccggg
    43021 cgataccatc gtcgcggtcg ccgatattta cggcggcgca tatgacttat tggtcgatgt
    43081 ttatcaaaaa tggggggtga acgttgtttt tgccgatttg ggcaatcccg ataatttgga
    43141 cgaattgctc aaagcgcaca atgtcaaact ggtttggctg gaaatgccgt ccaatccgct
    43201 tttacgcttg gtagacatca aagcccttgc agcgaaagcc aaagcagccg acgcgctggt
    43261 cggcatcgac aacacctttg ccacgccgta tctgcaacag cccttggata tgggttgcga
    43321 ttttgcattc cattccgcta ccaaatattt gtgcggccat tccgacgtat tgatgggcat
    43381 cgtcgttgcc aaaaccaaag agctggctca gcctttgcac gatatgatgg tgcataccgg
    43441 cgcgattgca ggcccgacgg actgctggct ggtgttgcgc ggcatcaaaa cgcttgccct
    43501 gcgtatggag gcacactgca aaaacgcact cgaaattgcg cgccgtttgg aagcccatcc
    43561 tgccattgaa aaagtgttcc atcccggcct gccgtctcac gaacattacg cactcgccca
    43621 agcgcaaatg cccaaaggca tcggcggcgt ggttacggtt tatctcaaaa acgacacgcg
    43681 cgaagcggca aacagcgtga ttaaaaacat gaaactggtc aaaatgacct ccagcctcgg
    43741 cggcgtggaa agtctggtca accattgcta ttcccaatct cacagcggcg tgccgcataa
    43801 tgtgaaaatg gaaatgggca tcagggtcgg actcctgcgc ttctccatcg gtattgaaga
    43861 cgtagatgat atttggaacg atatttccgc cgcactcgat acaactttgt aaaccgtaaa
    43921 aaatgccgtc tgaaaccata gtttcagacg gcatttcaat attgcgaatc aaatatcaac
    43981 gcttcagcat ctttgccgcc tcaatggcgt aataggtcaa aatcccgtcc gcacccgcac
    44041 gtttgaatgc cagcaggctt tccaaaacca ctttgccgcc gtccagccag ccgttggcaa
    44101 ccgccgcctg caacatcgca tattcgcccg aaacctgata ggcataagtc ggtacgccga
    44161 actcgtcctt cacgcggcgg acaacgtcca aatacggcaa accgggcttc accatcacca
    44221 tatccgcacc ttcctgaata tcgagcgcca cttcatgcag cgcctcatcg gtatttgcag
    44281 gatccatctg ataggtcttt ttatctgcct ttcccaaatt gcccgaactg cctaccgcat
    44341 cacggaaagg gccgtagaat gcagaagcat atttggcgga atatgccata atccgcgtat
    44401 ggatatgtcc ggcatcctcc aaagcctcgc ggatggcgcc gatacgcccg tccatcatat
    44461 cggaaggagc aacgacctgc gtgcccgcct ctgcatgaca taaagcctgt ttcaccaaga
    44521 cttctacggt ttcatcattc atcacgtaac cgttttcgtc cgtcagtccg tcctgaccgt
    44581 gcaccgtata aggatcgagc gcgacatccg tcataatgcc cagttcggga aacctctcgc
    44641 gcaaggctcg gacagttgac ggcacgagtc cttcgggatt gtacgcctcc tgcgcacgcc
    44701 cggttttgtt tgccgtaacc acgggaaaga gtgccaacat cggaataccg agcttcaccg
    44761 cctcttccgc cgtaaacagc agcctgtcca aactctgacg cttcacgccc ggcatagaag
    44821 gcacatcctc ctcgcgcgcc gccccctcca atacgaacac cggataaatc aaatcatcgg
    44881 cggtcagcat atgctcgcgc atcaggcggc gtgaaaaatc atccctgcgc atacggcgca
    44941 tacgcgaagc cggaacattg cggtaaggaa attgcataag ccctccaatc atcctgtcaa
    45001 caattcaaat catacggaag ccgccgcaac ttgcagacgg catcgagcgg taatcaaaat
    45061 ccgttttcac cggaaagggt ttcggcacgc cgaaatgccg cgccgaagga cagcgtgccg
    45121 cccattgaaa gaacaatagc aaaacaacca cttcaggctg tcaggcctcg atattcttac
    45181 gccacaatac caaaagtttc ccgatatgcc gaacaggttg cgcgtcaacc gcctcacaca
    45241 aggtattgca gatttcgata cgctcggcac ggtcgtcgcc gaatacgcgc actttaatca
    45301 gctcatgcgc cgtcagtgcc gcatcggttt ccttgatgac tgcgtccgtc agaccctgct
    45361 gaccgaccat cacaacagga tggagatggt gcgcgcgcgc tttcagttcc aaaatttctt
    45421 tggtgttcaa tttggtatcc gccattttcc tactgtttga ataaaaagga taatgcagca
    45481 ttgtacgcga tttggcacgg caacggcaaa attaacgtac aatacaccgt ttacattccg
    45541 acccgcgaac cactatggct gtacgttcca aatcctcaaa agcgtggctg cacgaacaca
    45601 tcaacgacca ctacgtccat atggcgcaaa aagacggcta ccgcgcccgt gccgcataca
    45661 aacttttgga aatcaacgaa aaagacaaga taatcaaacc cggcacggta cttgccgact
    45721 tgggcagcgc gccgggaagc tggtcgcagg ttgccgccaa gctgacgggt acttccggaa
    45781 cggttttcgc cttggacatc ctgcctatgg aagccatagg gggcgtgtcc ttcattcagg
    45841 gcgacttccg cgagaacgac gttttggcgc aattcgaaac cttgttggac aaccgcccgc
    45901 tcgaccttgt aatctgcgat atggcgccca atatgtcggg aaacgccgta agcgatcagg
    45961 cacgcagctt ttatctgtgc gaactggctt tggacttcgc ctcgcaacac ctgaaaaccg
    46021 gcggcagctt tttggtcaaa gtctttcagg gtgcaggcta tcaggaatac atggcagcca
    46081 tgcgcgaaat tttcggcact gtgcagacgc gcaaacccga agcctcgcgc aatcgctcca
    46141 gtgagattta tttattgggc aaaaataaac gctgacaata cagacggcgt gctttacaat
    46201 catttccgtt ttacacctca attatggagc cttgctaagt ggggaacacc tttaaatcaa
    46261 tccttgtctg ggtcgccttg ggtatcggcc tgatggctgc gttcaacgct ttggacggca
    46321 aaaaagaaga caacgggcaa atcgaatatt ctcagttcat ccgacaggtc aacaacggcg
    46381 aagtatccgg cgtcaacatc gaaggatccg tcgtcagcgg ctacctgatt aagggcgagc
    46441 gcaccgacaa aagcaccttc ttcaccaacg cgcccttgga tgacaacctg attaaaacac
    46501 tgctcgacaa aaacgtccgc gtaaaagtaa cgccggaaga aaaaccgagc gcgctgactg
    46561 ccctgtttta cagcctgctg cccgtcctgc tgctgattgg cgcatggttc tactttatgc
    46621 gtatgcaggc gggcggcggc ggaaaaggcg gcgcattctc cttcggcaaa agccgcgccc
    46681 gcctgctgga caaagatgcc aacaaagtta cctttgccga tgtcgccggc tgcgacgaag
    46741 ccaaagaaga agtgcaggaa atcgtcgatt acctcaaagc accgaaccgc tatcaaagcc
    46801 tcggcggtcg tgttccgcgc ggcatcctgc tggcgggcag cccgggaacc ggtaaaacac
    46861 tcttggcgaa agccattgca ggcgaggccg gcgtgccgtt cttcagcatt tccggttccg
    46921 attttgtcga aatgttcgtc ggtgtcggtg caagccgcgt ccgcgatatg ttcgagcagg
    46981 caaagaaaaa cgccccatgc attatcttta tcgacgagat tgacgcggta ggccgccaac
    47041 gcggcgcagg tttgggcggc ggcaatgatg agcgcgagca aacattaaac caattattgg
    47101 ttgaaatgga cggttttgag agcaatcaga ctgtaattgt gattgcggca accaaccgcc
    47161 ccgacgtact cgatcctgcg ctgcaacgcc ccggccgctt cgaccgccaa gtcgtcgtcc
    47221 ccctgccgga catccggggg cgcgaacaga ttttgaacgt ccattctaaa aaagtgcctt
    47281 tggacgaatc tgtggattta ttgtccctcg cgcgcggcac gcccggtttt tccggcgcgg
    47341 atttggcgaa cctggtcaac gaagccgccc tgtttgccgg ccgccgcaac aaagtcaaag
    47401 tcgatcaaag cgattttgaa gacgccaaag acaaaatcta tatgggtccg gaacgccgca
    47461 gtatggtgat gcacgaagac gaaaaacgtg cgacggcgta tcatgagtcc ggacacgcga
    47521 ttgttgccga aagcctgccc tttaccgacc ccgtccacaa agtaaccatt atgccgcgcg
    47581 gacgtgcgct gggtctgact tggcagcttc cggagcgcga ccgcatcagt atgtataaag
    47641 atcagatgtt gagccagctc tccatcctgt tcggcggacg gattgccgaa gacatcttcg
    47701 tcggacgcat ctccaccggt gcatcaaacg actttgaacg cgcaacccaa atggcacgcg
    47761 aaatggtaac gcgctacggc atgagcgaca aaatgggcgt gatggtttat gcggaaaacg
    47821 aaggcgaagt cttcttgggg cgcagcgtaa cccgttcgca aaacatttcc gagaaaaccc
    47881 agcaggacat cgacgcggaa atccgccgga ttttggacga gcagtatcag gttgcctaca
    47941 aaatcctcga tgaaaaccgc gacaagatgg aaacgatgtg caaagccctg atggaatggg
    48001 aaaccataga ccgcgatcag gtactggaaa ttatggcggg caaacaaccc agcccgccca
    48061 aggattacag ccacaacctg cgcgagaatg cggacgcggc ggaagataac gcgccgcacg
    48121 ctccgactcg ggaaaaaacc gaagcacctg ccccggcaga caccgcctcg acagagtccg
    48181 ggcagcagcc tgaaaacaag gcttaacttc ccgaacaaac ggcagcccgc aagctgccgt
    48241 tttcccatcc cgttttcaga cggcattttc cgctcaatgc cgtctgaaac gccaaagccg
    48301 tatcggtttt tgcgaatctg cgatatggcg gtttaaattg aaatcagaac ggggcaaggc
    48361 tgtaccggtt taaaattaaa ccgctataat accgcccttc gaatacacgc ccgcattggt
    48421 gatgcccgtg aaaaaagttg aaaaaaacat cttggtgctg cacggcgcgg acaaaatgtt
    48481 cgagctggtc gacaaggttg aggattaccc gcactttctg ccgtggtaca gcaagaccga
    48541 ggtcatcggg cgtagcggca acgaactgaa ggcgcggctg tttatggatt atatgcgcgt
    48601 tcgccaatcg tttgccacgc acaaccgcaa catccccggc agggaaatcc gtatggaact
    48661 gctcgaaggc ccgttcaaaa ccttacgtgg aacgtggaaa tttatcgatt tgggcgacga
    48721 tatgtgcaaa atcgaattca atttggaata cgatttttcc aatgccgttt tgtccgcctt
    48781 aatttccccc gtcttcaacc acctttccgc cacgctggtc gaagcgttcg tcaaagaggc
    48841 agaccgccgt tatgcttgaa attgagattg tgtacggtct gcccgaccga caggttttga
    48901 aaaccatgca gcttgccgag ggaacaaccg tccgcaccgc cgcactgcaa agcggtttgg
    48961 acggcatatt tgaaaattta aacctgcatt ccgcgccttt gggcattttc ggcaaagccg
    49021 tcaaagacga cacgccgctg cgcgacggcg accgcatcga agtgtaccgc ccgctgttga
    49081 tcgaccccaa agaagcgcgc cgcaaacgcg ttcaaaatca agaagaataa ccatgccgtc
    49141 tgaagccttc agacggcatt cgacagaaac acggaaaata tcatgtcaaa cacaatcaaa
    49201 atggttgtcg gcttgggcaa cccgggcaaa gaatacgaac agacccgcca caatgcgggc
    49261 ttttggttcc tcgacgaact ggcgtggaaa tggaaggctt cgtttaaaga agaaaaaaaa
    49321 ttcttcggcg aagttgcccg cgccgccctg cccgacggcg atgtttggct gctcaaaccg
    49381 gccacgttca tgaaccgttc cggacaggcg gttgccgcgc ttgcacagtt ctacaaaatc
    49441 aaacccgaag aaatcctcgt cgtccacgac gaactcgaca tcccttgcgg acggatcaaa
    49501 ttcaaactcg gcggcggcaa cggcggacac aacggcttga aagacattca ggcaaaactc
    49561 ggcacggcag actattaccg cctgcgcctc ggcatcggcc accccggcga ccgcaacctc
    49621 gtcgtcggct acgtcttgaa caaaccgagc gcggaacacc gccggcaaat cgacgatgcc
    49681 gtcgccaaat ccctgcaggc cgtacccgac atcatttccg gcaaatggga agaggcaacg
    49741 cgcttcctgc acagcaaatg acccgatgcc gtctgaagcc ctttcagacg gcattttccc
    49801 gatttccgta tccgaacagt catgaccgaa ctcaagcagc ttatccaaac cgaatccatc
    49861 cccgtcatcg aagaaaccct cgatttcctg ctgtacgaat gcagcatcga cgacgcaccg
    49921 tccgccgaag aagtggcaca atggcgcgac atacttgccg cacgcggcgg caaattcctg
    49981 cgcctgtcca aaatctgcca aacgtggctg gacgaggagg cggcatgaag ctgccgcgca
    50041 accgcttcag cctgctttcc gcattgtggt ttgccggcgg catctattcg ctgctcttca
    50101 aagctgccga caccgcgccg ccgccgtttc cacatttcga caaagcagca caccttgccc
    50161 tgtttttcgc acaaatcttg cttctggcca aagcattcaa aaccggaaaa cttcccatcc
    50221 cctaccgcag cctgattgcg ttcgccttct gttttgccgt cggcagcgaa tgcgcgcagg
    50281 catggtttac tgcaacgcga accggcagtt tgggcgatgt ccttgccgac ctgacgggcg
    50341 cagcccttgc cctctttgcc gcgcgttctg cctgccgccc ggactaaatc ggtttatttc
    50401 tcccaaacag gatgaatccc cctgatgcat cctgtttttc cccttacata tcaatcccat
    50461 ctcataaaca aaaccgacaa atcgtttaca atatatttac actacaccga attacaaata
    50521 tactcgaagc ggttgcagaa ccgacggttt cgcagaccgt ttcggattgc acgacacaaa
    50581 agaaaaaccg attttgttgc cttaaaggag cattcatgaa cctgcatgca aaggacaaaa
    50641 cccagcatcc cgaaaacgtc gagctgctca gtgcgcagaa gccgattacc gactttaagg
    50701 gtttgctgac caccattatt tccgccgtcg tctgtttcgg catttaccac atcctgcctt
    50761 acagccccga tgccaataaa ggtatcgcgc tgctgatttt cgttgccgca ctttggttta
    50821 ccgaagccgt ccacattacc gtaaccgcac tgatggtgcc gattctcgcc gtcgtactcg
    50881 gtttccccga catggacatc aaaaaggcga tggctgattt ttccaacccg attatctaca
    50941 tttttttcgg cggcttcgcg cttgccaccg ccctgcatat gcagcggctg gaccgtaaaa
    51001 tcgccgtcag cctgttgcgc ctgtcgcgcg gcaatatgaa agtggcggtt ttgatgttgt
    51061 tcctcgttac tgcctttctg tccatgtgga tcagcaacac cgccaccgcc gcgatgatgc
    51121 tgcctctagc aatgggtatg ctgagccacc tcgaccggga aaaagaacac aaaacctacg
    51181 tcttcctcct gctcggcatc gcctattgcg ccagcatcgg cggcttgggc acgctcgtcg
    51241 gctcgccgcc caacctgatt gccgccaaag ccctaaatct ggacttcgtc ggctggatga
    51301 agctcggcct gccgatgatg ctgttgattc tgcccttgat gctgctctcc ctgtacgtca
    51361 tcctcaaacc taatttgaac gaacgcgtga aaatcaaagc cgaatccatc ccttggacgc
    51421 tgcaccgcgt gatcgcgctg ttgattttcc ttgccacagc cgccgcgtgg atattcggct
    51481 ccaaaatcaa aaccgccttc ggcatttcca atcccgacac cgttatcgcc ctgagtgccg
    51541 ccgtcgccgt cgtcgtcttc ggcgtggcgc aatggaagga agtcgcccgc aataccgact
    51601 ggggcgtgtt gatgctcttc ggcggcggca tcagcctgag cacgctgttg aaaacatccg
    51661 gcgcgtccga agccttggga cagcaggttg ccgccacctt ttccggcgcg cccgcatttt
    51721 tagtgatact catcgtcgcc gccttcatta tttttctgac cgagttcacc agcaacaccg
    51781 cctccgccgc cctgcttgta ccgattttct ccggcatcgc tatgcagatg gggctgcccg
    51841 aacaagtctt ggtattcgtc atcggcatcg ccgcatcttg cgccttcatg ctgccggttg
    51901 ccacgccgcc taacgcgatt gtgttcggca cgggcttaat caaacaacga gaaatgatga
    51961 atgtcggcat actgctgaac atcctatgcg tagtattggt tgctctgtgg gcttatgctg
    52021 tactgatgta aaccatcgac ctaaacaaca agaccgtctg aaagaatatt tttcagacga
    52081 ccttgaagtt tgtcgttcag acacaatttg tcgaataatt caaaaccgga ttctaacgaa
    52141 aggaaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg aactcgattt
    52201 cgacaaagcc cctgttaccg ccaaaaactt cgagcaatac gtcaaagacg gcttctacga
    52261 cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg gcatggatga
    52321 aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca acggcctgcc
    52381 caacgataaa tacaccatcg ccatggcgcg cacttccgac ccccattccg ccggcgcgca
    52441 attcttcatt aacactgccg acaacgcttt cctaaacttc cgttctaaag agctgtacgg
    52501 caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg acggttttga
    52561 cgttgtcgat gccatcgaaa gcgtatccac caaacgccac ggctaccacg acgatgtgcc
    52621 gaccgaacct gtcatcatca ttaaagccga agcagtataa accggcaatc cgaaagcagc
    52681 ctgcataaaa ggctgctttt atttcagacg gcatgttatt tgatgtaacc gggctatgcc
    52741 gtctgaaaac cggacgcaag ccttcagacg gcataccgct atgttaaaat acgcccgttt
    52801 ttcctaaacg gacacaaagg aaaccttatg gcaagcatca cccgcgacat cttcaaagcc
    52861 tacgacatcc gtggcatcgt cggcaaaacc ctgaccgacg atgccgctta tttcatcggc
    52921 agggccatcg ccgccaaagc cgccgaaaaa ggtatcgccc gcatcgcgct cggacgcgac
    52981 ggacgcttga gcggccccga actgatggag cacatccaac gcggcctgac cgacagcggt
    53041 atcggcgtac tcaatgtcgg catggttacc actcctatgc tctacttcgc agccgtcaac
    53101 gaatgcggcg gcagcggagt gatgattacc ggcagccaca atccgcccga ttacaacggt
    53161 ttcaaaatga tgctcggcgg cgacacgctc gcaggcgaag ccattcaaga acttttagct
    53221 attgttgaga aagacggttt tgttgccgcc gacaaacaag gcagcgtaac cgaaaaagac
    53281 atctccggcg cataccacga ccacatcgtc ggacacgtca aactcaaacg cccgataaac
    53341 atcgccatcg acgcgggcaa cggcgtgggc ggcgcgtttg ccggcaaact ctacaaaggt
    53401 ttgggcaacg aagtgaccga acttttctgc gaagtggacg gcaatttccc taatcaccac
    53461 cctgatcctt ccaaaccgga aaacctgcaa gatttgattg ccgcgctgaa aaacggcgat
    53521 gccgaaatcg gcttggcgtt tgacggcgat gccgaccgct tgggcgtggt taccaaagac
    53581 ggcaacatta tttatcccga ccgccaactg atgctgttcg cccaagacgt tttgaaccgc
    53641 aatcccggcg cgaaagtcat tttcgatgtc aaatccacac gcctgcttgc cccgtggatt
    53701 aaagaacacg gcggagaagc cataatggaa aaaaccggcc acagcttcat caaatccgct
    53761 atgaaaaaaa ccggtgcact ggttgccggc gaaatgagcg gacacgtttt ctttaaagaa
    53821 cgctggttcg gcttcgacga cggcctgtat gccggcgcac gcctcttgga aatcctgtcc
    53881 gcctccgaca atccgtccga agtgttggac aacctgccgc aaagcatttc cacgcccgaa
    53941 ctcaacatct ccctgcccga aggcagcaac gggcatcaag ttatcgaaga actcgccgcc
    54001 aaagccgaat ttgaaggcgc aaccgaaatc atcaccatcg acggcctgcg cgttgaattt
    54061 cccgacggct tcggtctgat gcgtgcttcc aataccacgc cgattttggt gttgcgtttt
    54121 gaagcggata cgcaagcagc catcgagcgc attcaaaacc gattcaaagc cgtcatcgaa
    54181 agcaatccgc atttaatctg gcctctgtaa aaatagaaaa aatgccgtct gaaactttca
    54241 gacggcattt ttcacaagta gccaatcaaa tcttggcaga ttggatttgg cttaaaaatc
    54301 tccgcgtccg ttcgtgtttg aggtggtcga acaactcttt cgggctgccc tgctctacga
    54361 taacgccgcc gtccatcacg acgacgttcg tggcaacttc cagcgtgaac ttgatttcgt
    54421 gggtaacgac gaccatcgtc caaccttccc gcgccaattc cttcatggcg tccaacacgt
    54481 cttgcaccaa ctcggggtcc agcgcggaag tgggttcgtc aaacagcatc aattcaggct
    54541 gaatcgccag tgcgcgggcg ataccgacac gctgctgctg accgccggaa agctggtagg
    54601 gatacaaatc cactttatcg cccaagccga ctttttccag cagtttcaaa gcctcttcgc
    54661 gcgcttgggc ggcaggcttg ccctgtacgg caaccggccc ttccatcacg ttttccaaca
    54721 cggttttatg cgggaagagg ttgtattgtt ggaataccat tccggacttg cggcgcagtg
    54781 ccaaaatatc gtgtttgctt gtttttttgg aaaaatcaat gcgtaacggc cgcgcgttgt
    54841 cgaactcgat ttgtccgtct tcgggcattt ccaacgcgtt taggcagcgc agaaatgttg
    54901 ttttacccga gccggaaggc ccgaggatga cgaccacctg ccctttgccc acatccaaat
    54961 cgatgccgcg caaaatggtg ttttcgccaa aggtcttatg gatattgcgg attttaatca
    55021 tgacaactcc ttatttggcg acataacggt cgaaacgttt ttccaaacgc gcctgaatca
    55081 aaaacagcac tttacagaaa caccaataaa ccaatgcagc ttcgatatag acaggcaaaa
    55141 agtcataagt gcggtttgcc gtttcctgtg cgacacggaa aagctccgtt accgttacca
    55201 cggcggcaag cgaggtgttt ttgaacaagc cgataaactc gttgctcaac ggcggaacgg
    55261 cgacgcggaa tgcctgcggt gcgacgatgc ggcggaacgt ctgcatatag gtcataccga
    55321 tggagaaacc tgcttcccac tgccctttcg gcacggacaa aatcgccgcg cgtatggttt
    55381 cggaagcgta tgcgccgaca ttgagcgaaa agccgatgat ggcggcggga atcggattga
    55441 tatagatgcc gacggacggc agcccgtaaa acacaatcac aagctgaacc aacagcggcg
    55501 taccgcgaac gacggaaata taaaattcca ccagcttcaa caagcatttt tggaaaatac
    55561 cgccggaagg catgattctt accaaagcaa cggctacggc aataatcatg ccgataacga
    55621 aagaagcgat cgccaaaggc aaagacactg taaagccggc tttaaccatg ggccaaaacg
    55681 cgctgatgag catatcagcg cgtgtttccg tcataaacgg cagagaggca aggaaattat
    55741 tgaacactga tgtcttttcc gaagaattgt tcgcccagtt ttttcaacgt gccgtcggct
    55801 ttcagctcgt tgattgccgt gctgaatttc gccacggcct cgtcattgcc cttgttgaca
    55861 atcagaccgg aaccgacttt ttcatcggca ggcgcggacc acacgatttt cacccccgca
    55921 ttcgggtttt tcttcagata gtccaaaacc gccaattcat cgttcaacgt cgcatcggcg
    55981 cgtttttgtt caatcagggt cagcgattgc gccaaaccgt ccaccggcac gagttgcgca
    56041 cctgcggctt tggctttttc gccgtagttg ctggtcaggg attgcgcggt tttcacgcct
    56101 ttgatgtcgg ctatggattt aatgttgctg tcgttacgcg caaccaaaac cgcaccgctc
    56161 cagctgtaag gttcggattt gtcaaatgtc gcctggcgtt cggggctggt caggccgact
    56221 tggtttgcca ccacgtcgaa acgtccggct ttcaaacccg ccatcatcga atcccattgc
    56281 gtttctttaa actcgacttt tacgcccagt ttttccgcca cggcgcgggt tacttccacg
    56341 tcgtaaccgg tcagtttgcc gtctttgtcg tggtaggtaa acggtgcgta agtgccttcc
    56401 gtgccgacgg taaccgtgcc tttattgttg atgcgctcga ttaaagaacc ggaaattgcc
    56461 gattgtgcag gcgcggaaga tgctccgctg ccgccttccg aaccgccgca ggccgccaaa
    56521 accaatgcgg caatgccgcc gagtacgaat tttttcaaca tcattccctc ctgtaaaaag
    56581 tgtgcaagtg cggcattcta agtgaaattg cggcaaacac aaagaatact ttttttatat
    56641 ggttattcca aatcggaatg ggaaagatat gccgaagccg ccggaagcgg aaataaaggg
    56701 ggcgggaaag gcggcgggcg gcacgaaacc gaaaagcggc atcaggtatg caaacggcgg
    56761 attcacgcac gccgttccgg caaaaatccg cctgccgatt cggtgctata atccacccga
    56821 aacagatgac accaaaaagg aacatctcat gaacacgccc caatccgcca tcattcccga
    56881 ccacgcccaa gccggcatct ttatcgaagc cgacttcgcc gccaaccgcc tcaacgatat
    56941 taaagccgcc tgccgcgctt cgctcgacgc gttatccgcc ttgaaagccc gctttccgga
    57001 cgatattttg ggtctgacca tcgccttcgg cagcaaagct tgggcaacat tcggacacac
    57061 ggacgaaggc agtgaaatca aacccttccc cgaaatgggc aacgggcttg cgccgtccac
    57121 gcagcacgat atgtccatcc acatccaatc cttccgccaa aacgcagcct acgcgcttgc
    57181 ccaatccgtt ttgggcgcgt tcggcgacag catatgcgtc gcgtccgaag aacacggttt
    57241 gcgcctgtat caggatcgcg ggctggacgg tttcgtcgac ggcacggaaa acccgcaagg
    57301 cgatgaaaca atccgcgaag tcgccatcat ccccgaaggg ctgcccgatg caggcggcag
    57361 ctatgtcctg ctgcaaaaat acctgcacga tttgaaaaaa tgggatgccg tccccgtcgc
    57421 cgaacaggag gcctcggtcg gacgcagcaa ggaaaccgac gacgaattca gccgcgatgt
    57481 ccgcctgccc gattcgcacc tcggccgcgt caacctgaaa gaaaacggag tcggcctgaa
    57541 aatcgtccgc cgcagcctgc ccttcggcaa aatcagcggc gaacacggtt tgatgtttac
    57601 cgcatactgc cgcacgctgc ataatatcga agcgcaatta ttgagtatgt tcggcgacac
    57661 ggacggcaaa accgacctgc tgctccgaca cctctccgcc gccgtttcgg gcggatacta
    57721 ctacgcgccg tctgtcgaac gcctgcaaaa cctctaagcc ggacgatgcc gtctgaaacc
    57781 ctttttcaga cggcattttc ccgttttacc gtccaaccgt tttcatacac gctatgtccg
    57841 cacccatccg agcattcgac ccgctgaccg cccccatttc cggcaccaac ctgattgagg
    57901 cctctgccgg caccggcaaa acctacggca ttgccgccct gtttacgcgc ctgatcgtat
    57961 tggaacaaaa aagcgtcgag cgcgtattgg tcgttacctt caccaaagcc gccactgccg
    58021 agctgaaaac acgcctgcgc gcgcgtttgg acgatgtgtt gcaggttttg gaaagcaaag
    58081 aaattgccga acttggagac gacacgcttt cagacggcat tgccgcctac tgcgccgaac
    58141 accacgaagg cgacaccttc ctgcccgcac tcttggaaca ggctttgcaa aaagaaagcc
    58201 ggacgcgtct gattgtccgc ctcaaggccg ccatcgggca attcgacaac gccgccatct
    58261 acaccatcca cggcttctgc cagcgcatcc tgcgcgacta cgccttcctg tgccaagcac
    58321 cgttcgatgt cgaactgacc gaagaagacg gcgaccgcct gcttgtcccg gctcaagatt
    58381 tttggcggga acgcgtcagc ggcgacccgg tgcttgccgc attggcgttt aaacgcaaag
    58441 ctgtgccgca aaccgtcctt gcccaaatcc gcgcctacct gtcccgcccg tacctgaatt
    58501 tccgccgtcc gcaggcggat ttgaaacagg cgcagcgcga cgccgaaacc tcatggcaaa
    58561 ccgtctgccg cctgctgccc gagctggaag ccggcttttg gcgcatccat cccgacctca
    58621 acggcaacag ttaccgcaaa aacagcttcg gcaacctttt taaagaactg gcacaaaaat
    58681 ccgccgccgg acaactgccc tttctggaca aagacacgca cgaacggctc ttgaaactct
    58741 catccgacaa actcgaagcc ggactgaaaa aaggcaaaac gcccgatgcg gcagtatttg
    58801 ccgaattgca gaaactggca gacttcgggc gcgatttgaa cgcactcgaa gaagcggaag
    58861 aagcaacaat gatccggctg caactggatt taatcgaata tctcaacagc agccttgccg
    58921 aaatgaaaaa atcgcgccgc gaacgcggtt tcgacgacct gctgctcgat gtccacaccg
    58981 cgctgaccga caatccgcac gccgaaaccc ccgcccgcgc cgttgccgaa aactgggaaa
    59041 tcgcgctgat cgacgagttc caagacaccg acccgctgca atacgaaatc ttccaaaaga
    59101 ttttcatcgc ccgaaaccgc ccgctgttcc tcgtcggcga ccccaaacag gcgatttaca
    59161 gctttcgcgg tgcggacatt tacgcctacc ttcaggcggc ggaagacgcg cggcaccgct
    59221 acacgctcgc caccaactac cgcagccacg ccgcgcttat cggcagcatc ggcgcgctgt
    59281 tccgcctcaa agaacgcccg ttcgttttgg aaaacatcgg ctattcggaa gtcggtgcgg
    59341 cgcgtgccga aagcaggttg tcgcccaaac gtcctgccgt acagctccgt tggctgcacg
    59401 aaaacgacaa tgaaaaagcc aacaaagacg ttttgcgccg ccgtgccgcc gactattgcg
    59461 ccgacgaaat cgcccacgcg ctcaacgaag cctccggagg ccgtctgaat ttcaaaggcc
    59521 gcccgttgca gtcgggcgat attgccgttc tggtacgcac gcacaacgag gcggtgatga
    59581 tttccgccgc actgaaaaaa cggcaggtgc aaagcgtcct gctttcgcgc gaatccgtgt
    59641 tcgcctcgcc cgaagccgcc gccctgtccg cgctcatcgg cttctggctc gaaccgcgcc
    59701 gcgccggaac gctgcgcttt gtcctgacaa gcggcatatt cggttatgac gcgcagcaat
    59761 tgcacgactt caaccaaaac gaaagcgaga ttttgcattg ggcggaatct gcccgaaccg
    59821 cgctcgacat atggcaaaaa tacggcattt tcgccgctat gcagcagttt tcccaaacac
    59881 acggcatcga aacgcgcctc ttaagccgga acaacgggcg cagcctgacc aattatttcc
    59941 aactgctcga actgcttgcc gccgaagacg cgcaaaaccg caaccccgcc gcactgcaca
    60001 aatggctgcg cgatcaaatc agcctggccg gcaacaacgg cggcgacaac cgcgccatcc
    60061 gtttggaaag cgacgaagat ttggtcaaaa tcgttaccat gcacgcctcg aaaggtttgc
    60121 agtatccgct ggtgtactgc ccgtttgcgt gggacgcgca agataccgga ccgtccgact
    60181 ggcaaatcct ccaccaaggc gcaaaccggg ccgaactttt ggcaaaggcg caactgtcgg
    60241 aaagcgaaca gaagcaatac gccgacgaag aaatggcgga acgcctgcgc ctgctttatg
    60301 tcgcgctgac gcgtgccgag gaacagctca acatctacgc cgcctattct tccgataccg
    60361 ccgacaaccc ccttgcctac ctgatcgaag gcttgccgca agacagccgc gaaaccgtcc
    60421 gccgtaccta tgcgtgtgaa aaagacggca taacgatgct caaacgcaac tggcggcgcg
    60481 tggcggacaa tgccccggcc ggcacggatt tcgccttcac agaagatgcg ccgccccctg
    60541 ccgcctatcg cggcaacgcc gatcaagccg ccgaatttgc cgcaaacagc attcccgaac
    60601 gcggattccg atttgtccgc cacaccagct tcaccgcctt aagccgccaa acccaaacgc
    60661 ccgacggcgg cgaagaagat gcctgcccgt ccttggatgc cgccgaaacc tcggtgccgg
    60721 cgatgccgtc tgaaacgccg acggcttcag acggcatatc gatacacgac tttccgaaag
    60781 gcacgcaggc ggggctgtgc ctgcacgaaa ttcttgaaga tttcaaattc ggacaagcag
    60841 ccgccgaaca ggaaaccctc attgccgaca agctgaaaaa atacggtttt gaagaaatat
    60901 ggctgcccgc cgttgccgaa atggcggaag cctgccgcaa aacgccgctg actggggcat
    60961 acggcctgtc cgacatcccg cccgggtgcc gccgtcccga aatgggcttt accctccaca
    61021 ccgaagactt cggcctcaaa cggctgcgcg actggcttgc ccgcgacgac atcaggctgc
    61081 ccgaagtctg ccgtgccgcc gccgagacgc tcgacttcca caccgtcaac ggctttttaa
    61141 acggctttat cgatatggtc tgccaagacc ccgacggcaa tatctgcatc atcgactaca
    61201 aatccaacca cctatccgcc tacacgcggc aggcgatgga cgaagccgtc gcgcaccagc
    61261 actattacct tcaggcactg atttacgccg ttgccgccgc gcgctacttc aaactgcgcg
    61321 gacaaccgcc cgccgccgtt tccgtccgct acctgttttt gcgcggattg gatggcaaag
    61381 gcggcggcgt ttggcgttgg gacatagatg ccgccgcttt ggaacagata aaataacttc
    61441 ccgtccctta agacgacaac cgcaggagaa ccctatgaat atcaaacaat tgattaccgc
    61501 cgcactcatt gcctcagccg cctttgccac gcaggcagcc ccgcaaaaac ccgtatccgc
    61561 cgcccaaacc gcgcaacatt cagccgtttg gatcgatgtc cgttccgaac aggaatttag
    61621 cgaaggtcat ttgcacaacg cggtcaacat ccccgtcgac caaatcgtcc gccgcatata
    61681 cgaagccgcg cccgacaaag acacgccggt caacctctac tgccgcagcg gacggcgtgc
    61741 cgaagccgcc cttcaagagc tgaaaaaagc aggttataca aatgttgcca atcacggcgg
    61801 ttatgaagac ctgctcaaaa aagggatgaa atgacgcaat gccgtctgaa aggacaggct
    61861 ttcagacggc atacggcgtt ccggcacgcg gcacggcatc aggcgcggcg cgtgccgccc
    61921 cgtccgaagc ccgataaaca aaccgataag gaaatacgat gaaacacata ctccccctga
    61981 tcgccgcatc cgcactctgc atttcaaccg cttcggcaca ccctgccgac aaaccgccca
    62041 cccaaaacga aaccgctatg accacgcaca ccctcacctc gaaatacagt tttgacgaaa
    62101 ccgtcagccg ccttgaaacc gccataaaaa gcaaagggat ggacattttt gccgtcatcg
    62161 accatcagga agcggcacgc cgaaacggcc tgaccatgca gccggcaaaa gtcatcgtct
    62221 tcggcacgcc caaggccggt acgccgctga tggtcaaaga ccccgccttc gccctgcaac
    62281 tgcccctgcg cgtcctcgtt accgaaacgg acggcaaagt acgcaccgcc tataccgata
    62341 cgcgcgccct catcgtcggc agccgcatca gtttcgacga agtggcaaac actttggcaa
    62401 acgccgaaaa actgatacaa aaaaccgtag gcgaataagc ccgtcgccga catatggcgg
    62461 atggaaatca aatgcacacg cccctcattc ccccgaagtc gggaatccaa gcggttgggt
    62521 taccgtgatt tccaatcatt gccgaaacgg acgattccgg attcccgcct tcgcagggat
    62581 gacgggcgta tgcgttttgc catatcacag gcaactttgc cgcaaatctt atctgtcgcc
    62641 ggtatgcctt cttgtttcaa tccgccacaa accaaatgcc gtctgaaacc tgatttcaga
    62701 cggcatttcc gtttattccc tgctgtcgcg gcaaatatcg atggcttcct gcaaacttga
    62761 aacgccgtgg attttcaggt tcggaaactc tttggcattg cgcggcatat tggctttggg
    62821 gacgatggcg cgtttgaagc cgagcttttc cgcttctttg agccgctctt gtccgcgtgc
    62881 gacggggcgg acttcgccgc ttaggccgat ttcaccgaag accacggttt tttcaggcat
    62941 agggcggttg cggaagctgg aaagcatcgc gaggatgacc gccaaatccg ccgccggttc
    63001 gccgattttc acgccgccga cggcgtttaa aaaaacatcc tgatcgaaac aggcgatgcc
    63061 gccgtgccgg tttaacacgg caagcagcat cgcaaggcgg ttttgttcca atccgacgct
    63121 gaggcgtttg ggtgtgaagc cgtgcgcgtc atcgaccaat gcctgaattt cgaccaaaag
    63181 cgggcggctg ccttcctgtg taaccaaaac gcacgaaccg ggcgtgtcgt cgcggtagct
    63241 ggcgaggaag atggcggacg ggttggatac gcctttcaaa ccgttttcgg tcatcgcgaa
    63301 cacgcccaat tcgtttgccg cgccgaaacg gtttttgatg gcgcgtatca tgcggtagtt
    63361 ggaatgttgg tcgccctcga aatacagcac ggtatcgacc atatgttcca gcacgcgcgg
    63421 gccggcaatc gctccgtctt tggtaacgtg tccgaccagt atcatggcga tacccatttg
    63481 tttcgccata cgcgtcagtt gggcggcaca ttcgcgcacc tgcgacacgg aaccgggggc
    63541 ggacgtgatt tggtcggaat acatggtttg gatggaatcg ataacgacga cttcgggctg
    63601 atgctgtttc aaggccgcct gaatcgcttc catgcggatt tcggcaagca ggtttacgcc
    63661 ttcggcgtgc agttccaaac gttgcgagcg cagggcgacc tgttgggcgg attcttcgcc
    63721 ggaaacgtac agcactttgc ggctttgcgc cattttggcg atggtttgca acaacagcgt
    63781 ggatttgccg atgccgggat cgccgccgag caggatgacc gcgccatcga ccaaaccgcc
    63841 gcccaatacg cggtcgagtt cgcccatacc ggtcggattg cgcggcactt cggcagcggt
    63901 aacggcggag agggattgga cggtcgaagc gtccgccgcc caagattgga atcgggcgtt
    63961 tttcggctcg ggcgcggcaa agctttcctg aagcgtgttc cactcgccgc aatgcggaca
    64021 tttgccctgc catttcgggg aagtgccgcc gcattcggtg cattggtaaa gggttttaag
    64081 cgtttttgcc atgatgtttt ccctatggga tttttgaagt gtaaaaaaat cgtctgaaac
    64141 ttcttttcta aaaaattttt tattattagt ttgttaattt tgattacaag accatatcac
    64201 gatacaaaca agggtggctt tctgttattt tgcggcataa tgacggaaaa tacaaaacag
    64261 gaatcccaaa caatgacttt cccattaaat ataatggtag a
//
LOCUS       DAXDZG010000002        63209 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXDZG010000002 DAXDZG010000000
VERSION     DAXDZG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63209
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.002"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACFB78_000061"
     CDS             complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACFB78_000061"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC1268164.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACFB78_000062"
     CDS             complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACFB78_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC1268165.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFIRYKVGEGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACFB78_000063"
     CDS             complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACFB78_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC1268166.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2936..3169)
                     /locus_tag="ACFB78_000064"
     CDS             complement(2936..3169)
                     /locus_tag="ACFB78_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268167.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3247..3828
                     /locus_tag="ACFB78_000065"
     CDS             3247..3828
                     /locus_tag="ACFB78_000065"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC1268168.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3889..4479
                     /locus_tag="ACFB78_000066"
     CDS             3889..4479
                     /locus_tag="ACFB78_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC1268169.1"
                     /translation="MSERIILSVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4863..6329
                     /locus_tag="ACFB78_000067"
     CDS             4863..6329
                     /locus_tag="ACFB78_000067"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC1268170.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6516..6887)
                     /locus_tag="ACFB78_000068"
     CDS             complement(6516..6887)
                     /locus_tag="ACFB78_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC1268171.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(7125..7424)
                     /locus_tag="ACFB78_000069"
     CDS             complement(7125..7424)
                     /locus_tag="ACFB78_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1268172.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            complement(7523..8302)
                     /gene="map"
                     /locus_tag="ACFB78_000070"
     CDS             complement(7523..8302)
                     /gene="map"
                     /locus_tag="ACFB78_000070"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC1268173.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8342..8992)
                     /locus_tag="ACFB78_000071"
     CDS             complement(8342..8992)
                     /locus_tag="ACFB78_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268174.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(9000..9608)
                     /locus_tag="ACFB78_000072"
     CDS             complement(9000..9608)
                     /locus_tag="ACFB78_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC1268175.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9672..10265)
                     /locus_tag="ACFB78_000073"
     CDS             complement(9672..10265)
                     /locus_tag="ACFB78_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC1268176.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            complement(10270..10617)
                     /locus_tag="ACFB78_000074"
     CDS             complement(10270..10617)
                     /locus_tag="ACFB78_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC1268177.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10664..11539
                     /gene="rsmI"
                     /locus_tag="ACFB78_000075"
     CDS             10664..11539
                     /gene="rsmI"
                     /locus_tag="ACFB78_000075"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC1268178.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGGLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11574..12104)
                     /locus_tag="ACFB78_000076"
     CDS             complement(11574..12104)
                     /locus_tag="ACFB78_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC1268179.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12383..13537
                     /gene="obgE"
                     /locus_tag="ACFB78_000077"
     CDS             12383..13537
                     /gene="obgE"
                     /locus_tag="ACFB78_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC1268180.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13591..14538
                     /locus_tag="ACFB78_000078"
     CDS             13591..14538
                     /locus_tag="ACFB78_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1268181.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14535..15587
                     /locus_tag="ACFB78_000079"
     CDS             14535..15587
                     /locus_tag="ACFB78_000079"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC1268182.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15603..17024
                     /gene="cysS"
                     /locus_tag="ACFB78_000080"
     CDS             15603..17024
                     /gene="cysS"
                     /locus_tag="ACFB78_000080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC1268183.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGRSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17195..17974
                     /locus_tag="ACFB78_000081"
     CDS             17195..17974
                     /locus_tag="ACFB78_000081"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC1268184.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17961..18305
                     /locus_tag="ACFB78_000082"
     CDS             17961..18305
                     /locus_tag="ACFB78_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268185.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18315..18845
                     /locus_tag="ACFB78_000083"
     CDS             18315..18845
                     /locus_tag="ACFB78_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC1268186.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18938..20053)
                     /gene="asd"
                     /locus_tag="ACFB78_000084"
     CDS             complement(18938..20053)
                     /gene="asd"
                     /locus_tag="ACFB78_000084"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC1268187.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20228..20809
                     /locus_tag="ACFB78_000085"
     CDS             20228..20809
                     /locus_tag="ACFB78_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC1268188.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20871..21725)
                     /gene="folD"
                     /locus_tag="ACFB78_000086"
     CDS             complement(20871..21725)
                     /gene="folD"
                     /locus_tag="ACFB78_000086"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC1268189.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            21948..22025
                     /locus_tag="ACFB78_000087"
     tRNA            21948..22025
                     /locus_tag="ACFB78_000087"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21983..21985,aa:Pro,seq:tgg)
     gene            22050..22126
                     /locus_tag="ACFB78_000088"
     tRNA            22050..22126
                     /locus_tag="ACFB78_000088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22084..22086,aa:Arg,seq:tct)
     gene            22154..22229
                     /locus_tag="ACFB78_000089"
     tRNA            22154..22229
                     /locus_tag="ACFB78_000089"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22187..22189,aa:His,seq:gtg)
     gene            22365..22871
                     /gene="rfaE2"
                     /locus_tag="ACFB78_000090"
     CDS             22365..22871
                     /gene="rfaE2"
                     /locus_tag="ACFB78_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC1268190.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            22868..24646
                     /locus_tag="ACFB78_000091"
     CDS             22868..24646
                     /locus_tag="ACFB78_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC1268191.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEKGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRSVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            24759..25583
                     /locus_tag="ACFB78_000092"
     CDS             24759..25583
                     /locus_tag="ACFB78_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC1268192.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(26090..26302)
                     /locus_tag="ACFB78_000093"
     CDS             complement(26090..26302)
                     /locus_tag="ACFB78_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC1268193.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGSFVV"
     gene            complement(26317..27105)
                     /locus_tag="ACFB78_000094"
     CDS             complement(26317..27105)
                     /locus_tag="ACFB78_000094"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC1268194.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(27184..27378)
                     /gene="thiS"
                     /locus_tag="ACFB78_000095"
     CDS             complement(27184..27378)
                     /gene="thiS"
                     /locus_tag="ACFB78_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC1268195.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLHENDKIDIVRPVVGG"
     gene            complement(27800..28417)
                     /gene="thiE"
                     /locus_tag="ACFB78_000096"
     CDS             complement(27800..28417)
                     /gene="thiE"
                     /locus_tag="ACFB78_000096"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC1268196.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(28439..29539)
                     /locus_tag="ACFB78_000097"
     CDS             complement(28439..29539)
                     /locus_tag="ACFB78_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC1268197.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(29536..30762)
                     /gene="cytX"
                     /locus_tag="ACFB78_000098"
     CDS             complement(29536..30762)
                     /gene="cytX"
                     /locus_tag="ACFB78_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC1268198.1"
                     /translation="MSGNASSPSSSAAIGLVWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(30828..30932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            31007..32491
                     /gene="tldD"
                     /locus_tag="ACFB78_000099"
     CDS             31007..32491
                     /gene="tldD"
                     /locus_tag="ACFB78_000099"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC1268199.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELFAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            32635..33294
                     /locus_tag="ACFB78_000100"
     CDS             32635..33294
                     /locus_tag="ACFB78_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1268200.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            33284..33952
                     /locus_tag="ACFB78_000101"
     CDS             33284..33952
                     /locus_tag="ACFB78_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1268201.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            33954..34685
                     /locus_tag="ACFB78_000102"
     CDS             33954..34685
                     /locus_tag="ACFB78_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1268202.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            34798..35652
                     /locus_tag="ACFB78_000103"
     CDS             34798..35652
                     /locus_tag="ACFB78_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1268203.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            36068..36889
                     /gene="prmC"
                     /locus_tag="ACFB78_000104"
     CDS             36068..36889
                     /gene="prmC"
                     /locus_tag="ACFB78_000104"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC1268204.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            36975..38363
                     /locus_tag="ACFB78_000105"
     CDS             36975..38363
                     /locus_tag="ACFB78_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC1268205.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            38364..38588
                     /locus_tag="ACFB78_000106"
     CDS             38364..38588
                     /locus_tag="ACFB78_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC1268206.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(38988..39881)
                     /locus_tag="ACFB78_000107"
     CDS             complement(38988..39881)
                     /locus_tag="ACFB78_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC1268207.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            39924..42626
                     /gene="ppc"
                     /locus_tag="ACFB78_000108"
     CDS             39924..42626
                     /gene="ppc"
                     /locus_tag="ACFB78_000108"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC1268208.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDALTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            42749..43738
                     /locus_tag="ACFB78_000109"
     CDS             42749..43738
                     /locus_tag="ACFB78_000109"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC1268209.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            43792..44121
                     /locus_tag="ACFB78_000110"
     CDS             43792..44121
                     /locus_tag="ACFB78_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268210.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            44132..44437
                     /locus_tag="ACFB78_000111"
     CDS             44132..44437
                     /locus_tag="ACFB78_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC1268211.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            44443..44622
                     /gene="ssrS"
                     /locus_tag="ACFB78_000112"
     ncRNA           44443..44622
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFB78_000112"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            44800..45231
                     /gene="rplM"
                     /locus_tag="ACFB78_000113"
     CDS             44800..45231
                     /gene="rplM"
                     /locus_tag="ACFB78_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC1268212.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            45244..45636
                     /gene="rpsI"
                     /locus_tag="ACFB78_000114"
     CDS             45244..45636
                     /gene="rpsI"
                     /locus_tag="ACFB78_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC1268213.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(46017..46511)
                     /locus_tag="ACFB78_000115"
     CDS             complement(46017..46511)
                     /locus_tag="ACFB78_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268214.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKGIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKGLDSEKIIS
                     LNYD"
     gene            complement(46704..47633)
                     /locus_tag="ACFB78_000116"
     CDS             complement(46704..47633)
                     /locus_tag="ACFB78_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC1268215.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            47762..48511
                     /locus_tag="ACFB78_000117"
     CDS             47762..48511
                     /locus_tag="ACFB78_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC1268216.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GKPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            48634..49215
                     /gene="petA"
                     /locus_tag="ACFB78_000118"
     CDS             48634..49215
                     /gene="petA"
                     /locus_tag="ACFB78_000118"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC1268217.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            49234..50583
                     /locus_tag="ACFB78_000119"
     CDS             49234..50583
                     /locus_tag="ACFB78_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC1268218.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            50598..51386
                     /locus_tag="ACFB78_000120"
     CDS             50598..51386
                     /locus_tag="ACFB78_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC1268219.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            51718..53508
                     /gene="rmuC"
                     /locus_tag="ACFB78_000121"
     CDS             51718..53508
                     /gene="rmuC"
                     /locus_tag="ACFB78_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC1268220.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(53575..54207)
                     /locus_tag="ACFB78_000122"
     CDS             complement(53575..54207)
                     /locus_tag="ACFB78_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC1268221.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTRNVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            54299..55123
                     /locus_tag="ACFB78_000123"
     CDS             54299..55123
                     /locus_tag="ACFB78_000123"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC1268222.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGIGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            55192..55755
                     /locus_tag="ACFB78_000124"
     CDS             55192..55755
                     /locus_tag="ACFB78_000124"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC1268223.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            55839..56276
                     /locus_tag="ACFB78_000125"
     CDS             55839..56276
                     /locus_tag="ACFB78_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC1268224.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            56497..56766
                     /locus_tag="ACFB78_000126"
     CDS             56497..56766
                     /locus_tag="ACFB78_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC1268225.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            56766..58541
                     /gene="ptsP"
                     /locus_tag="ACFB78_000127"
     CDS             56766..58541
                     /gene="ptsP"
                     /locus_tag="ACFB78_000127"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC1268226.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(58668..59609)
                     /locus_tag="ACFB78_000128"
     CDS             complement(58668..59609)
                     /locus_tag="ACFB78_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1268227.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(59618..60493)
                     /locus_tag="ACFB78_000129"
     CDS             complement(59618..60493)
                     /locus_tag="ACFB78_000129"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC1268228.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(60676..60752)
                     /locus_tag="ACFB78_000130"
     tRNA            complement(60676..60752)
                     /locus_tag="ACFB78_000130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60716..60718),aa:Met,seq:cat)
     regulatory      60901..61000
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            61091..62992
                     /gene="thiC"
                     /locus_tag="ACFB78_000131"
     CDS             61091..62992
                     /gene="thiC"
                     /locus_tag="ACFB78_000131"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC1268229.1"
                     /translation="MTTPKKTAKTSGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT        14498 a        16535 c        17799 g        14377 t
ORIGIN      
        1 tgaaaaacaa attgtatcaa aattgtaaat ttaatccact ataatctcca tataataaat
       61 ggctgtctga agcccatatg cgactatatc tgccgaccct atggtcaatc atcaatcagc
      121 agtggaccag tgtgccttcg tcttcgccgg taatgacgcg tttgagcgag ccttgtttgg
      181 cgatgccgaa gacgacgata ttgagcttgc gttcgcggca gagggcaaag gcggtcgcgt
      241 ccatgacttt gaggttttta ttcaaggctt cgtcaaaagt aatggtttcg tagcgtgtgg
      301 cggacgggtc ttttttcggg tctgcggtgt acacgccgtc gacgttggtg gctttgagca
      361 tcacgtcgca gttcatttcc gcgccgcgca atgcggcggc agtgtcggtc gtgaagaacg
      421 gattaccggt accggcggcg aaaatcacga ctttgccttc ttccaaatat tgaatggctt
      481 taggacgggc gtaagtttca gcgatttgct gcatagacag tgcggattgt acgcgcgctt
      541 tgatgcccaa ggtttcaaat gcgtctttga gtgccaaggc gttcatcacg gtcgccatca
      601 tgcccatgta gtcggcagtg gcgcggtcca tgctgcctgc ttgtgcagat acgccgcgga
      661 aaatgttacc gccgccgaca acgataccga cttgcacacc cattttcacg atttcggcaa
      721 tttcgccgac agtttgaaca atggtatcgt gattgatgcc gaacggatcg gaacccatca
      781 gggattcgcc ggagagtttc agtaatacgc gtttgtattt gatttgctgt gtcatgggat
      841 accttgcttt cttgaatgtc gtcggtcgga gttgggacgt ctgaatttca aatggtcgga
      901 ttacaatgat atggtgtgtc gttctattat tgttttgttt tcagacggcc tttatattcg
      961 gtcgtcttaa aataggtacc gtaaacggtt ttctcaaaaa aaagcaccct gattcgtttg
     1021 gaatccaggt gctttctttt tcataagtgc cttacacttt ggcggctgcg gctacttcgg
     1081 ctgcgtagtc gacaacggct ttctcgatac cttcgcctac tttgtagcgg atgaagctaa
     1141 ccacttcagt gtcgttttct ttagcgaatt gggcaacagt ttggtcaggg ttcatcacga
     1201 atgcttggcc gttcagagtg atttcagcca agaatttgcg gatacggcct tcaaccattt
     1261 tagcggcgat gtcggcaggt ttgccggaag cgatggcttg ctcggtgtag atgtggcgtt
     1321 ctttttcaac ggtttcagca tctacttcgg cttcgcttac gcattgcggt ttggcggcaa
     1381 cgatgtgcat accgattttg cgtgctacat cttcagagcc tttgtactcg accaaaacgc
     1441 cttcggtcgc caatgcgccg tggatgtagg caaccagttg gttggcagtg tcaatcactt
     1501 ggaagcggcg gacagacatg ttctcgccca atttagcgat gatggctttg cgttctgatt
     1561 caaccagttc gctcagttct tcaacagaag ccggtttttt ctcggcagca gttttcgcaa
     1621 cgaagttggc aaattctacg aagccggcgt ctttagcaac gaagtcggtt tcgcagttta
     1681 cttcgaccaa tgcgccgaca ttaccgttga ttgcataagc caatacgcct tcggcagcag
     1741 tacgaccagc cagtttgccc gctttcgcac cggatttaat acgcaggatt tcttcggctt
     1801 tgtcgaagtt gccttcagct tcaaccaagg cttttttgca ttccatcata cccaggccgg
     1861 tagcggcgcg caggtcggca accatttttg cagtaatttc tgccattttg aatctcctag
     1921 atttttggga aatacggaaa ggattttcca cattcgggaa tcgtgtgccg cgaacgggcg
     1981 ttcagacggc atatttgaga aaaggggcat aacgcccctc ttctgtgtac cgaattactc
     2041 ggcagcggtt tcttgggcag cggctacggt ttcttgcagc gcttggtttt tgccttccaa
     2101 aactgcgtcg gcgatgcctc ggcagtacag gcggatggct ttggcggagt cgtcgttgcc
     2161 ggggatgacg tatttcacgc cgtctgggct gttgttggta tcgactacgg cgataacggg
     2221 gatgcccagt ttttcagctt caaccagagt acctttttgg tagccggtat cgataacgaa
     2281 aatcgcgtcg ggcaggcctt tcatgttttt gataccgccc aaagagcgtt ccagtttttc
     2341 aacgtcgcgt tgcatttcca gaatttcttt tttgctgaaa ccgctttcgg cagcattttc
     2401 caaggctgcg gttttttctt ccaggcgttt gatggattgc ttaacggttt tgtagttggt
     2461 cagcataccg cccaaccagc ggtggtcgac gaaaggcata ccggcgcggg tcgcttcttc
     2521 gcggatgatg tcgcgtgcct ggcgtttggt acctacgaac aataccgtac ctttgttggc
     2581 aaccagacga cgtacggctt cttgcgcctc ttggaacatc ggcagggttt tttccaagtt
     2641 tacgatatgg attttgttgc gcgcgccgaa aatgtattga gccattttcg ggttccagaa
     2701 acgggtttgg tgaccgaagt gaacaccggc ttcaatcatc tgacgcatag taatttgaga
     2761 catgttattt ccttgaaagg gttaaagagc acacattcaa tcgcatagaa cttgataggc
     2821 ggcgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa atagtgtgtt
     2881 tccgaaatgg aaaactgacg gattatagcg gataagatgc cgtctgaaaa gggattcagg
     2941 gttttgtgcc gctgtctttt tgccgttgtg cgcgctgctg ttcggattct gcctgttcct
     3001 gctgccccat caggcggttg cggcggcgga cggttacggc gaagaccgtg aacacggtgg
     3061 gcagaaccac ccaaaaaacc aaataaatca aagcgcgcgc aatactcggc tgcgcggcgg
     3121 aatacataac ggcaacaaaa agatagccga tggcgacgat gtgccacatt gtgtgcgtcc
     3181 tgtttgaatg ttaacaaaat cgttataatc gcaacattct accgcaatcc tgaaaggccg
     3241 tctgaaatga ggaacgagga aaaacgcgcc ctgcgccgcg aattgcgcgg gcggcgttcg
     3301 caaatggggc gggacgtgcg ggcggcggcg gcggtaaaaa tcaaccgcct gctcaaacgt
     3361 tatatcaagc gcggtcggaa aatcggcgtg tattggccga tgggcaagga attgcgtttg
     3421 ggcggctttg tccgcgcggc gcaaaaacgc ggcgcaaaac tctatctgcc ttatatcgaa
     3481 ccgcacacgc ggcggatgtg gtttacgccg tatcctgaac gcggaatgga acgggaacgc
     3541 aagcgcggta gggcgaagct gcatgtccct cagtttgcag ggcgcaaaat ccgcgtgcac
     3601 ggtttgtcgg tattgctcgt cccgcttgtc ggcatagacc gcgaaggcta ccgtttgggg
     3661 caggcaggcg gctattacga tgcgacgctt tcggcgatga aataccgttt gcaggcgaaa
     3721 accgtgggcg tgggctttgc ctgccagttg gtggacaggc tcccacgcga ggcgcacgac
     3781 ctgccgctgg acggttttgt atcggaagcg gggatattgt gtttttagcc gttcagacgg
     3841 catttcgggc ggaaatgccg tctgaaggcg gaatattggg agaaaaggat gtcggaaagg
     3901 ataatactgt cggtcttgtc gttgggcggc gtacggctcg aaccgttgga tgtgcatcat
     3961 gaaacgggtt tgcgcgaggc ggtttgcgat ggggaggttt ggaagctggg ggtaacgtcc
     4021 gcgccccatc cggatagggt ggcagactat atcgggacag cattggcaac gcgtttggcg
     4081 tttgcggttg tcgatgaaga ggcggacagg gtggtcggga caacggcgta ttatcatttt
     4141 gaaccgcaga ttccgcgttt ggatatcgga ttcacatggt atgcggcatc ggcacggcgg
     4201 aaacggataa acacctgttg caaaatcatg ctgttggatt acgcatttga tgttttagct
     4261 tgctgttgcg cgggatggcg cacggatatt ctgaaccttg cctcgcaacg tgccatcgaa
     4321 cggctgggcg cggaaaaaga cggcgtgctg cgtatgcata tgctccgtaa agacggcagt
     4381 gtgcgcgata cggttgtgta cagtatgctg agggaagatt ggtgcaaaaa cagggaaatc
     4441 ctgaccggca ggttggcagg gtatggcgtg caggtgtgag gcagtattct caaatttaaa
     4501 aaacttaacg cgatatgctg acaatctttt aaaaattaat atataaacaa ttttgatttt
     4561 ttgtctatta aaaatttgat atatatagat aaataactat ttttattttt tgtttcgcag
     4621 ttccccggct tggcgggcga cagaaaaact ttgcatttcc cagtaaaatc cactacattc
     4681 acgataacat attacaagcc cctgaagcgg tgtcggaagc catacttgca acgcattcca
     4741 accccgaaac agcgtgctcg gacacacacc attgccggtt tcccctgctt ttgcgattca
     4801 ggcgtttgta gtacggcaaa taaccataag ggcagaagat gcctgttttt taggagtaaa
     4861 ccatggctga agcgacagac gttgtcttgg tgggcggcgg cattatgagc gcgactttgg
     4921 gcgttttgct caaagaactc gaaccgtctt gggaaatcac cctgattgaa cgcttggaag
     4981 atgtggcgtt ggaatcgtca aacgcgtgga acaacgccgg cacggggcat tccgcgctgt
     5041 gcgaattgaa ctatgcgccg ctgggtgcgg acggcgtcat caatccggcg cgcgccctca
     5101 atattgccga acagtttcat gtcagccgcc agttttgggc gacgctggtc gcggaaggca
     5161 agttggaaga caattccttc atcaatgccg tgccgcatat gtctttggtg atgaacgaag
     5221 accactgccg ttacctgcaa aaacgctatg atgtgtttaa aacgcagaaa ctttttgaaa
     5281 atatggaatt ttccaccgat cggaacaaaa tttccgattg ggctccgctg attatgcgcg
     5341 gccgggacga aaaccaaccc gtcgccgcca actattccgc cgaaggcacg gatgtcgatt
     5401 tcggacggct gacgcgccag atggtgaaat atttgcaggg caagggcgta aaaaccgagt
     5461 tcaaccgcca cgtcgaagac atcaaacgcg aatccgacgg cgcgtgggtg ctcaaaaccg
     5521 ccgatacccg caacccagac tggcagctca ccctccgcac ccgcttcctc ttcctcggcg
     5581 cgggcggcgg cgcgctgacc ctgctgcaaa aatccggcat ccccgaaggc aaaggctacg
     5641 gcggcttacc cgtgtccggc ctgttcttcc gcaacagcaa ccccgaaacc gccgaacaac
     5701 acaacgccaa agtgtacggg caggcttccg tcggcgcgcc gccgatgtcc gtcccgcacc
     5761 tcgacacacg caacgtagac ggcaaacgac accttatgtt cggtccttac gcaggtttcc
     5821 gttccaactt cctcaagcaa ggctcgttta tggatttgcc gctgtccatc catatggaca
     5881 acctctatcc tatgctgcgc gccggctggg cgaatatgcc gctgaccaaa tacctgctgg
     5941 gcgaattgcg taaaaccaaa gaagaacgct ttgcctccct gctggaatac taccccgagg
     6001 caaaccccga cgactgggaa ctcatcaccg cagggcaacg cgtccaaatc attaaaaaag
     6061 actccgaaaa aggcggcgtg ctgcagtttg gtacggagat tgtcgcccac gccgacggct
     6121 cgctcgccgc attgctggga gcgtcgccgg gcgcatcgac cgctgtgccg ctgatgatcc
     6181 ggctgatgca ccaatgcttc cccgagcgcg ccccgtcttg ggaaggtcgt ctgaaagagc
     6241 tggtaccggg ttacggcatc aagttgaacg aaaaccctga aagggcggat gaaattatcg
     6301 cctataccgc gaaagtgttg gatatttaaa ccgaatcttt caagaaaagc cgtaccccca
     6361 tatggtgttg acgccgtccc tgccggttat gcccttcatc cggcagggca ggttttgata
     6421 cacggggcag gtaatcgttt tttccctttt gcgaataatg gaacggtcaa aaaacagcgt
     6481 tttcagaaat gaaaacgctg ttttttgttg cctgattaac gtggggaaca gtctttgaag
     6541 acgatttggt tgtcaggtgc ggtaatcata ataggctgtt tgcggtagga tttgctgtcc
     6601 attgcgccgg tgctcagcac ataaccgcct tctttgccgt agaacgtgtc catattgtcg
     6661 gatttatcca aattgatggg catttgcaca cgtttgccgt tgatgacggc ggaggcgtat
     6721 gtggtcagac cctgtttgtt gaagccgtag gttactttga cttttttacc ttgctggcag
     6781 acgtagctga cggttttttt ggcaacggtg gggttgtccg tgccggcggc ggccatactg
     6841 ctgagcgcga ttgcggaaga caggattgca gtggtcagaa gtttcatttt tattcctttg
     6901 gatagatgtg gagtttgccc gtttcggact gtttgacagt ctaatgtcgg cgtatcctga
     6961 aataaacagg aaattggata aagtttgtaa aaaggcaggt tcgggcggtt cggcggtttg
     7021 aggcaggggc ggcaggcggg atacggcggc ctgtccggtg tttcagacgg cattgtttgc
     7081 atttttcgga aatgaaaaac ctccgggcat ccggaggttg cggtttaacc ttgttttttt
     7141 ctttcggcct tggtctgttt ggacaattcg gcatacaggt cgggatggtg ttttttcaac
     7201 agggcggcga tttccatatc ttttttgttg acttttgcca aatcgaggca ttcgatatgg
     7261 acgaggcgga gcagttcgcg tacgggtttg ggcatcttgc ggccggtttc gtagcgtgaa
     7321 cctccggatt gagtaacgcc gaccttgccc cagaagtcta tctggttcat cccggtcttt
     7381 ttacggatgg ctttaacatc ttctatattt tcaaatattt tcataaaact tcctttaagt
     7441 ttttctggtt gcggggcaaa ttccgatttg ctttgttttc gtaatgtgcc gggtcaagcc
     7501 tgtttttata ttgcatacag cttcagggtt tgccggtggc ggggctgacg gtgaggattt
     7561 cgtagccggt ttcggttacc aagacttcgt gttcccattg ggcggagagg gagcggtctt
     7621 tggtaaccac cgtccagccg tcgttgagga tacgcaggtg gcgtttgcct tggttgatca
     7681 tcggttcgac ggtaaaaatc atacccggtt ttagaacggg accctgccct ttttttccgt
     7741 agtgcaacac ttgcggggct tcgtggaaac cgcgcccgat gccgtgtccg cagaattctt
     7801 gtacaaccga ataacctgca ttttcggcaa cctgctggca ggcgtaacct acgccgccca
     7861 gcgtcgcgcc aggtttgacg gcttctatgc ccgccatcat agaggcgtgg gttacgtcga
     7921 tcaggcgttg ggcgatgggg gagactttgc cgacggtaaa catacggctg gagtcgccgt
     7981 ggaaaccgtc ttttttgatg gtgaggtcga tgttgataat gtcaccttct ttgagcggct
     8041 tgtcgtcggg aatgccgtgg cagatgacgt ggttgacgga ggtgcagcag gatttggggt
     8101 agggcgggtt gccgtagtgg aggggggcgg gatagccgcc ttggacgttg acgtggtagt
     8161 cgtaaacaag tttgtcgatt tcgtcggtgg tgatgccggg tttgacgaat tgtccgatgt
     8221 agtcgagggc ttcggcgacg agtttgccca gctcgcgcat tttttcgatt tcttcggggg
     8281 ttttgatgat gatgccgttc ataatgcttc cttggattgc gggtgtgcct gccgtgccgg
     8341 gtcaaaagag gttgtcgaga atctctttat ggggtttggc cggcgtgttt ttcggggtgt
     8401 ccggtttggt atggttttct ttgggcgttt ctttttcttt gggtgtttct ttgggctgcg
     8461 cctgtttttc gggtgtttct tttcggggtt cgggatgggg ggcgtttgtg ggcagtacgg
     8521 gggtttcttt gagtgtgttt tcagcttgtg ttccggtgcc ggttaacgaa tcggctgccc
     8581 gggccgcatc cgaagggcgc ggtttgaccc ggtttttctt gggtgcggca gggggcggca
     8641 gtccgctgcc gctttcttct gtgccgtccg gctgtgcggg ttcggatgag gcggcgtggt
     8701 ggtgttcggg ttgggcggca tcttgtccga cggcgccgtt tggcagccag aattcggttt
     8761 cgcgggggct gtcggtgtgt tgcggttcgg tttggagggt tttgtcgccg ttcgggttga
     8821 atgtgctgac gatgcctact gccaatgcgc cgatggcggt gatacagatg agcaatacgg
     8881 cgcgtatcag gagtttgggg gtcagcctga agggtttgtt cggttttttt gccattttga
     8941 ttgtgctttt ggggtgtcgg gcaatgccgt ctgaaggcgg ttcagacggc attgccgagt
     9001 cagcgttgga cgtagttttg gtagagggta atgacttttt gtacgccgac ggtggtgctg
     9061 actttttggg taatctgcgc ctgttcttcg ggggtgagga tgcccataac gtaggttaca
     9121 ttgccgtagg taatgatttt gacgcgcgcc tgtgtagcgg ggctgatgcc cagcagcgtg
     9181 gcgcggactt tggacgtgtt ccaagtgtcg ccggcgatgt cgcccgcagt gcgcggcagg
     9241 gaggcgacgg taatgtagtt gtatacgcct tcggcggcct gttcggaacg tgcaatctga
     9301 ccgacgaact gtttttcgcc ttcggtggcg acttgtccga gcagcagcag gtggcggttg
     9361 tagccgacga cggagatttg gggcgtgtag cctttggttt ggttgttttg gcgcaggtag
     9421 gaacgggcgg tggtttcgat acgcaacgcc ataacgttgt catcggtttg cgcgccggtg
     9481 gttcggcggt cgatgacgga tttcgcgccg acggcggccc cgccgacgac tgcgctgaag
     9541 cagccgccga gggcaaggct gaggacggcg gcaatcaggg tgcggacggt gtgtggtttg
     9601 ggtttcatcg gggacttcct ttcttgggcg tttcagacgg cattgctttg cgccatgccg
     9661 tctgaaaagg gttacattcc ttccagcagt acggagtcga tacagtcgca tatggcgtgt
     9721 atcagcagga tgtggttttc ctgaatgcgg gcggtgcgcg gatgggggac gttgagcaga
     9781 acgtcggtgt ctttgagcat ggcggcgatt ttgccgccgt cgcggccggt cagggcgatg
     9841 acgtgcatat cgcgttcgtg cgcggctttg acggcttcga tgacgttggc ggaattgccg
     9901 gaggtggaaa tgccgacgag gacgtcgcct gcacgtccga gcgcgcgcac ttgtttgctg
     9961 aatacgtggt cgaaaccgta gtcgttgccg atggcggtca gcgcggaggt gtctgtggtc
    10021 agggcgacgg cggcgagttc catgcgttct ttctcaaaac gcccggtcat ttcggcggcg
    10081 aagtgttgcg cgtcggcagc cgaaccgccg ttgccgcagg ccaggatttt gccgtcgttc
    10141 atcaggcatt gcagcatcag ctcggcagcc tgtacggtcg gctcgaccag tattttttcg
    10201 gcttcctgtt tggcacggat gctttcggca aaatgtgcgg caacgcgttc ttgtaatgtc
    10261 gtcatatcgt caacctgtaa tattctttat ccactcgggc gggcggtttc cttcgataag
    10321 taccgcatcg aggcggcacg gtacgtttgt cagcctgttc tgttgcagat aatactctac
    10381 acttcgttgc agtttcaata atttggacgg agaaatgctg tatgccgcgc cgccgaaccg
    10441 ttggtttttg cggtatttta cttcaacaaa cagaatcatg ccgccgtttt tgacaatcag
    10501 gtcgatttcg ccgtaggcgc agtgccagtt gcgggcaagc agcgtgcagc cttgggattg
    10561 gaggaaggca agtgcggcat cttcccctgc cgtaccctgt ttgtggttta agcgcataat
    10621 cggtttaacc tttataatgt ctgtttttca gacggcattt tttatgtttc agaaacactt
    10681 gcagaaagcc tccgacagcg tcgtcggagg gacattatac gtggttgcca cgcccatcgg
    10741 caatttggca gacattaccc tgcgcgcttt ggcggtattg caaaaggcgg acatcatttg
    10801 tgccgaagac acgcgcgtta ctgcgcagct tttgagcgcg tacggcattc agggcgggtt
    10861 ggtcagtgtg cgcgaacaca acgagcggca gatggcggac aaggtaatcg gtttcctttc
    10921 agacggcctg gttgtggcgc aggtttccga tgcgggtacg ccggccgtgt gcgacccggg
    10981 cgcgaaactc gcccgccgcg tgcgcgaagc agggttcaaa gtcgttcccg tcgtgggcgc
    11041 aagcgcggta atggcggcgt tgagtgtggc cggtgtggcg gaatccgatt tttatttcaa
    11101 cggttttgta ccgccgaaat cgggcgaacg taggaaattg tttgccaaat gggtgcgggc
    11161 ggcatttcct gtcgtcatgt ttgaaacgcc gcaccgaatc ggggcaacgc ttgccgatat
    11221 ggcggaattg ttccccgaac gccgtctgat gctggcgcgc gaaatcacga aaacgtttga
    11281 aacgttctta agcggcacgg ttggggaaat tcagacggca ttggcggcgg acggcaacca
    11341 atcgcgcggc gagatggtgt tggtgcttta tccggcgcag gatgaaaaac acgaaggctt
    11401 gtccgagtct gcgcaaaatg cgatgaaaat ccttgcggcc gagctgccga ccaagcaggc
    11461 ggcggagctt gccgccaaga ttacaggtga gggcaaaaag gctttgtacg atttggcact
    11521 gtcgtggaaa aacaaatgat gccggatgga ccggcaccat agtgcgaacg gaatcaacgg
    11581 acacgccccg tcatgcggta cagcacgtct ttgccttgaa cacgatggac gacgaccatg
    11641 gcgacgtgtc cgacgacggc ggcaaacagc agccagccca aattgccgtg gaacgtgttg
    11701 cccaagtttg ccatccactc gattttttcc ggcgaaccct gcattacttc aacgccgaac
    11761 actttcaacg ggccgcgtcc gccaccgtat tggcggatca tgccgataac gggaacggca
    11821 agcatgagca gatacagaat gccgtgtcct gccgctgccg ccttgctgtc gctttgcgga
    11881 cgcttggcgc ggttggcaac cgcccacacg atgcgcaatg caatcaccgt cagcatgagg
    11941 aaaccgaaag atttgtgcag gccgaacagg ctgcccgccc attccgcctc ttcgtaaatt
    12001 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
    12061 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa atccttatca
    12121 tatgtaacgg tttgaacgga cattccgtcg ggtcggaatg tcaaaaaggc gcgattgtac
    12181 caaagaagtt ggacatattt tgtttgcagg ctgaagattt gcttaaaaat tcattaagat
    12241 gggcagaaca aatagcttct ccgcggtata ttgaaatacc gcttatacct tataatgtcg
    12301 gcagaaaatc tgttcagacg gcatcaaaac atatggcaat ccgcctgaaa ccctatttaa
    12361 aaccaacaaa caagagcatt taatgaaatt catcgacgaa gcaaaaatcg aagtcgccgc
    12421 aggcaaaggc ggtaatggcg caaccagttt ccgccgcgaa aaattcgtac cccgcggcgg
    12481 cccggacggt ggcgacggcg gcaaaggcgg cagcgtctgg gcagaagccg acgaaaacac
    12541 caacaccctc gtcgaatacc gcttcgtcaa acgctaccaa gccaaaaacg gcgaaaaagg
    12601 ccacggttcc gaccgctacg gcgcaggtgc ggacgacatt gtcctcaaaa tgcccgtcgg
    12661 cacccttatc cgcgacctcg acaccgacga aatcgttgcc gacctcacct accacggaca
    12721 gcgcgtctgc ctcgccaaag gcggcaaagg cggcttgggc aacatccact tcaaatcctc
    12781 cgtcaaccgc gccccaaaac aatccacgcc cggcgaagaa ggcgaaaccc gttccctgca
    12841 actcgaactc aaagtcctcg ccgatgtcgg cttgttaggc atgcccaacg ccggcaaatc
    12901 caccctgatt accgccgtct ccgccgcacg ccccaaaatc gccaactacc ccttcaccac
    12961 cctgcatccg aacttgggcg tggtacgcat cgacgaaaac cacagcttcg tgatggccga
    13021 catccccggt ctgattgaag gcgcggcaga aggcgcaggc ctcggccacc gcttcctcaa
    13081 acacctatca cgcaccggtc tgctgttaca cgttgtcgat ttggcaccct tcgacgaaac
    13141 cgtcaatccc gccgaagaag cactcgccat catcaacgaa ttacgcaaat acgacgaaga
    13201 actttacggc aaaccgcgct ggctggtgct gaacaaactc gatatgctcg acgaagaaga
    13261 agcccgggcg cgaacagccg ccttcctcga agccgtcggc tgggactacc ccgaacctga
    13321 tgaccgtttc caattcgaca tggaaacccc gcgcctcttc caaatcagcg cgctgacaca
    13381 tcaaggcacg caggagttgg tacaccaaat caaccaatac ctggccgaga aaaaacgtat
    13441 cgaggctgaa aaagccgaag cggagaaagc agcggcaaat gttgaaatta tcgaacagca
    13501 gcctaaaacg gatactggcg tgtttaagcc ggagtaaaag gacaaggccg tctgaaagtt
    13561 ttcaggcggc tcattctgca gagatgtgct atgtacaaaa caatagattt attttcaggc
    13621 attggcggaa tacgcctagg ctttgaaaaa tacgggtgca ccaatgtatt ctcatcggaa
    13681 tgggacaaat atgcccgaca agtgtatgaa gcaaactttg gagaaaaacc ttttggcgac
    13741 attaacggca tcgatccttc agatattccc gatcatgata ttctgcttgc gggatttccg
    13801 tgccaacctt tcagtatcgc aggtaaggga ttgggatttg aagatacgcg cggaacattg
    13861 tttttcaata ttgccgaaat cctgaaaacc aaacagccga aggcttttct tctggaaaac
    13921 gttaagcggc tgactacgca cgattcgggc aggactttcc gtattgtttt agaaaccctt
    13981 aaacagcttg gttatacggt ttattttaag gttttaaata ctttggattt cggattgccg
    14041 caaaaacgcg agagaattta tattgtgggt ttttcagaca acatcccttt ttactttccc
    14101 gaacccataa accaatatcg gccattgggc gaactgctgg aaaacgatcg agatgtcgaa
    14161 ccaagctatt ttttatcaga taccttgaaa caaaaacgtt tggcagcctt aaaaaaagct
    14221 ccgcccaccc catcaatttg gcatgaaaat attggcggga atgtttccgc acttccatat
    14281 tcctgtgcct taagggccgg gggaagttac aattatttgg tggtaaacgg agtaagaagg
    14341 ctaacaggaa gggaaatgtt aagattacaa ggttttcccg atgattttga aatcaatatc
    14401 ccctactcgc aagtcaggaa agtcgccgga aattctgtat ccgtccctgt aatagaagcc
    14461 atcgcggaaa atatgctcgc ttctctttcc ggcaaggtcg aacaaaaagg gcaattggat
    14521 ttattggagg caggatgacg cttgaagaac aacaagccaa agaagcattg gacggcatta
    14581 tcaaaaaatc ccgtgtccac ctttataaac ccattcaaat tgcagaaatt ttatatcatg
    14641 accgttgtat aaaacagttg gattttttaa atttagatac ttaccgcaac caatctaaac
    14701 gctggaggga tgaaatctgc cgtcggtttt tagggcggat ttctacttca tctgcaaaat
    14761 tccaagataa tctgtttgaa aaaaatgcaa taccgcctga aaaactagcg gtattgggaa
    14821 cattaaacag acagtcggac ggcggggtag aatcgtacat ttataaacag tttttcaatc
    14881 gtttttccca aatgagcgaa gcattggctt atgtcggcaa tacagacaga tactccttcc
    14941 aactgtctga atttctaaat ttattttggc tcgagccggg attgaaaaga agtatagaca
    15001 agatatatga aatcgttgtt tatgcgttgt ttgatgcatt ggtttcagaa ttaggcataa
    15061 cggtttcaat tgattttcct aaggaaaacc tgtttttatg ggaggaatat caagattttg
    15121 ccgaaaaaat catcactatg ccgaaaaatg agcatttaaa acttcctgca aaaatccatc
    15181 gtgtaggcgt aaccaatgct gccgatagag ggttggatat gtggtctaac ttcggattgg
    15241 ccatacaggt caaacatctc tctttagacg aagagttggc agaagatatt gtatcctcca
    15301 ttagcgcaga tagaattgtc attgtctgca aaaaggcaga acaatcggtg attgtttcat
    15361 tactgacgca aataggctgg aaaagccgaa ttcaaaacat cgtaactgag gatgatttga
    15421 taagttggta cgaaaaagca ttgagaggcc aatatccgat tgcagaagcg ttgttggaaa
    15481 atattaaaac tgaaattatg cgtgaatttc ctgccgttaa tgaagccaat gaatttttag
    15541 atttcgccca aaatcgcgga tatgacatta ctgttactca tttctaaact tacttgatga
    15601 atatgaccgc catctacaac acccttaccc gtcaaaaaga acccttcgcc cccatcgacc
    15661 ctgaaaacgt gcgtatgtac gtctgcggca tgaccgttta cgactattgc cacttaggcc
    15721 atgcccgtgt gatggtggtg ttcgacatga ttgcccgctg gttgcgcgag tgcggttatc
    15781 cgctcactta tgtgcgcaac atcaccgaca tcgacgacaa aatcatcgcc cgtgcggctg
    15841 aaaacggcga aaccatcggc gaactgaacg cgcgtttcat tcaggcgatg cacgaagatg
    15901 ccgatgcttt gggcgtgttg cgtcccgaca tcgagccgaa ggcgacggaa aacatcccgc
    15961 aaatgattgc catgattgag accctgattc aaaacggcaa ggcatatcct gccgcaaacg
    16021 gcgacgttta ctacgccgtg cgcgagtttg ccgcttacgg acaattatcg ggcaaatcgt
    16081 tggacgacct gcgtgcgggc gaacgcgtgg aagtggacgg tttcaaacgc gatccgcttg
    16141 attttgtatt gtggaaagcg gcgaaagcag gcgaaccggc gtgggaaagc ccgtggggca
    16201 acggccgtcc gggttggcac atcgaatgtt ccgcgatgag tgaaaacctg ttcggcgata
    16261 cgttcgacat tcacggcggc ggcgcggatt tgcagttccc gcaccatgaa aatgaaatcg
    16321 cccaaagcgt cggtgccagc ggtcacacct gcggtcatga ccacgcgcaa acccaccacg
    16381 gtcgaagtat tgccagccac gtcaaatact ggctgcacaa cggcttcatc cgtgtggacg
    16441 gcgaaaaaat gtccaaatcg ctgggcaact tctttactat ccgcgaagta ttgaaacaat
    16501 acgaccccga agtcgtgcgt ttcttcatcc tgcgcgccca ctaccgcagc ccgttgaact
    16561 actccgacgc gcatttggac gacgcgaaag gcgcgttgac ccgtctgtac accaccttga
    16621 aaaacacacc ggcggctgag tttgatttgt ctgaaaacgc caacggctac acccgccgtt
    16681 tctacgccgc catgaacgac gatttcggta cggtcgaagc ggttgccgtg ttgtttgagc
    16741 tggcaggcga agtgaacaaa accaatgacg cacacctcgc cggctgcctg aaagctttgg
    16801 gcggcatcat cggcctgctg caacgcaacc cgattgagtt cctccaaggc ggcgcagttt
    16861 cagacggcct ctccaacgaa gaaatcgaag acttgattgc ccgacgcaaa caggctcgcg
    16921 cggataaaaa ctgggcggag tccgaccgca tccgcgacct tctgaacgaa cacaaaatca
    16981 ttttggaaga cagcgccggc ggcacgactt ggcggcgcgg ttgaactgta tcggaaaggg
    17041 caaaagtacc gccttttgcc cttttttcgt tttcagacgg catttcccgc ttgaagcccc
    17101 gccgttttca gcaggcagga ccgaatggga aataccgggc ggcgcaccgg gtgccgcttt
    17161 gtgtatggga acattgtgag aacttatttt tattatgctt aaaatcattt ccgccaacgt
    17221 caacggcatc cgctccgcct acaaaaaagg tttttacgaa tacatcgccg catcgggcgc
    17281 agacattgtc tgcgtgcagg aactcaaagc gcaggaggct gatttgtctg ccgatatgaa
    17341 aaatccgcac gggatgcacg gctattggca ttgcgccgag aagcgcggtt acagcggtgt
    17401 ggcggtgtac agcaaacgca aacccgacaa tgtgcaaatc ggcatgggca ttgaggaatt
    17461 cgaccgcgaa gggcggtttg tgcgttgcga tttcggcagg ttgggcgtca tttcgcttta
    17521 tttgcccagc ggcagcagcg cggaagaacg ccagcaggtg aaataccgtt tcctcgatgc
    17581 gttttaccct atgctcgaag cgatgaaaaa cgaagggcgc gacatcgtcg tctgcggcga
    17641 ctggaacatc gcccaccaaa acatcgacct gaaaaattgg aaaggcaatc agaaaaattc
    17701 gggcttcctg cccgaagagc gcgagtggat aggcaaagtc atccatacgc tgggctggac
    17761 ggacatgtgg cgcacgctct atcccgatgt gccgggttat acatggtgga gcaaccgcgg
    17821 gcaggcgtat gcgaaagatg tcgggtggcg catcgattat cagatggtta cgcccgaact
    17881 tgccgccaaa gccgtgtccg cacacgttta taaagatgaa aaattctcgg atcacgcccc
    17941 tttagttgtg gagtatgact atgctgccga ataaggtttt gggtaaatat gattggaatg
    18001 tggacggaaa aacaggaata ggcgcggcat gggttgtcgc tgcgtttatc ctgcccatat
    18061 tggtgtgggc ggtgtttatg ttgtcgcgga tgcaaggctg gcttgcgccg accaaggcaa
    18121 acccgatatg ggcgttggtg tggctgctga tttgtctgcc ctgcctgctg attgcggcca
    18181 aatgtttggg ttggaaaggc tggcggcggg tggtgaatat ttttgtctgc ctgaccgtct
    18241 gcgccatatt gagcgtaccc gcttcgctgc tcatcgcctt taccctccgg gacctgctca
    18301 aatagtcgcg gacaatggaa agtttcgagc ttgaggattt gacgctgtgg ctgatacgcg
    18361 atgcggatga agcggaaatg tggatagacc gttgggcggt cagttatccc gttgtgcaga
    18421 tgtccgaagc gtccgcaaac ggcagcacgg aagcgtggca aagcgcgctt cagacggcat
    18481 tcgaacggat acagggcagg catatcgccg ttgtcgcaca cggcgcgggc gcggccgcat
    18541 tcttggcgtg gctgtaccgc gccgacatcc tgacgcagaa gaaactcgcc ggtatcattc
    18601 tcgtatctcc gcgtcccgat atttttcccg acgatgcgga acacactttc caacgcgtcc
    18661 gctgtccttg ccgtgccgca ttggtggtgt ccgaacacgg cggcgtgccg cacggttggg
    18721 cgcaaaaaca ggcggatttg tggaacgccc gcctgttggt ttccccgcat tcgggcagtt
    18781 tgaacggtat gctcggcggc tggcagtggg gtatgaagct gatgcaggaa atgttgctgg
    18841 cgtgaacggt cgctttatcg gaatttcggt taaccggggt tgcaaggaaa ataccgtctg
    18901 aaacaggctt cagacggcat ttccattcaa acagggctta caggctgccc aacacgatac
    18961 gcaacacgcg gcgcatcggt tcggctgcac cccacaaaag ttggtcgccg acggtgaacg
    19021 cgctgatgta ttcgccgccc atgcccagtt tgcggatgcg tccgacaggg acggacagcg
    19081 tgccggtaac tttggcgggc gtcagctcgc ggatgctggc ttctttttcg ttggggacga
    19141 ctttcaccca gtcgttcgcg cctgccaaaa tcgcttcgat ttcggaaaca ggcaggtctt
    19201 ttttcaactt cagagtgatg gcttggctgt ggcagcgcat cgcgccgatg cggacgcaca
    19261 ggccgtcgat gacggtcgga ttgccgctgc gtccgaggat tttgttggtt tccacgccgc
    19321 ccttccattc ttctttggac tggccgttgc ccaaatccac gtcaatccac ggaatcaggc
    19381 tgccggcgag cggtacgccg aagttggctt tcggatagtc ttcgctgcgg aggaaatcgg
    19441 acactttgcg gtcgatatcg agaatcgcgc tggaaggatc ggcaagctcg tccgccactt
    19501 gggcgtgaat cgcgcccatg ccgctgatga gttcgcgcat atttttcgcg cccgcgccgg
    19561 aagcggcttg gtaggtcatg ctggtcgccc attcgaccaa atcgttttgg aacaggccgc
    19621 ccaaagccat cagcatcagg gagacggtac agttgccgcc gatgtagttt ttcacgccgt
    19681 ttttgagacc gttgtcgatg acgttgcggt tgacggggtc gagggcgata atcgcgtcgt
    19741 ctttcatgcg cagtgaggac gccgcgtcaa tccagtagcc gttccagccg ctgtcgcgca
    19801 gggcttggaa gacggatttg gtgtaatcgc cgccttggca ggtgacgata atgtccattt
    19861 tcgccagctc ggcaacgtcg ttcgcgtcca ataatgtttt agccgcctga ccgaaatcag
    19921 gtgccgcgcc gccgacgttg gaagaggtaa agaaaaacgc ttcgggaatg tgggcgaagt
    19981 cgttttcttc tttcatacgc tgcatcaaaa ccgaaccgac cataccgcgc cagccgacga
    20041 aacctacttt catgtattgc tccttgagga aaatcgttaa taaaattatg tatggcgtaa
    20101 agaaagcgtt ttaacgccgt attgggaaaa tgtaaagttc tttatcgaat tttctttttg
    20161 ggattggcgt ttgcagcagt agggcggtag aattccatct gtttaagaac aattatcagg
    20221 aacggatatg gaagaaaaaa acgactacac cgacgcggta tccgacaacc gcaacgggca
    20281 ggaaattgaa gtcggaattg ccggcgcagg cgaccggata cttgccgcgc tgctgaacca
    20341 attgtttacc tttttgattt tgttggtgcc gtttgtcgga ctgattgcct tcgccgtcaa
    20401 aaatgaaggc aggattggcg gccgtgagga aatattcggg ctgcttttgg gcatgacttc
    20461 cttttgggtc ggactggcgg gcattttggc gtacaccgtc atccaaatct actatatgag
    20521 cagggacggg cagtcattgg gtaagaaaat catgagaatc cgcgtgttga aaaccgacgg
    20581 ccgcaatccg ggttttgtcg gcacggtttt ggtacgcgaa atcgcgtggt cggttttggt
    20641 tgccattatt gccgccgtta tcggtcttgc agtaggtgaa aacggggaaa acgccatcaa
    20701 cctgctggca ttccttgcca actttgtcct gctctttatg gtcaaacgcg accgccgcac
    20761 gctttacgac atattggcgg atacggttgt cgtcaagctg cccaaataaa cggacgcggc
    20821 aaaatgccgt ctgaaaggct ttcagacggc atttttatct tcagaaccgc tcaagcatcg
    20881 tgcagcgaag ctgcgtgcaa agtgttttcc attaatgtgg caatcgtcat cggacccacg
    20941 ccgccgggaa cgggcgtaat catcgccgcc cgttcttttg ccgtttcaaa ttccacgtcg
    21001 ccgcacaggc tgccgtcgtc caaacggttg atgcccacat cgataacgac cgcgccgggt
    21061 ttgatccacc cgcctttgac aaagttcgga atgcccacgc cgaccaccaa aatatcggcg
    21121 gcggcaactt catcggcaag attttcggtc gcactgtggc agaccgttac cgttgcgcgc
    21181 gccagcagca attccaacgc ctgcgggcgg ccgacaatat tcgacgcgcc gaccacgacc
    21241 gcttttttcc ccttcggatc aatgccgtaa gcctccaaaa gcgtcatcac gcccttgggc
    21301 gtacacgggc gcatcagcgg cattttgacc gccagcctgc cgacattgta aggatggaag
    21361 ccgtccacgt ccttatccgg cgaaatacgt tccaagatcg cttggctgtc gaggtgcttc
    21421 ggcagtggaa gctgaaccag aataccgtcc acttcggaat cggcattcag acggtcgacc
    21481 aatgccagca gttcttcctg cgatgttgat tcgggcagtt cgtacgacag cgacttgatg
    21541 ccgcattttt ggcaggcggt tttcttgttg cggacataaa ctgcgccggc agggtcgcct
    21601 ccgaccaaaa ccacggccag gcaaggcgtg tgcagattgt cctgttggcg ttgcgccacc
    21661 gcttcggcaa tcgcctgcag gtgtttttgc gaaacttctt taccgttgat cagttgtgcc
    21721 gacatatccg tccttttgtt aaacaggtag aaaatgctga aaattatcgc attttccgtt
    21781 tgtttttaca gcagaatgcc gaaaataaat ttgccccgaa ataaaatacg gctgtttgga
    21841 cggattaaat attttttcaa atcaaaaaac tatatttttc atcttggtgt tttgaaaata
    21901 aatgttgacg catctttccc tgaactgtat attccgcttc ttcaagtcgg ggcgtagcgc
    21961 agcccggtta gcgcatctgc tttgggagca gagggtcgtg agttcgaatc ccaccgcccc
    22021 gaccaatcaa tcagcacgaa tcggcatgag cgcccgtagc tcaaccggat agagcaccga
    22081 ccttctaagt cgggggttac aggttcgatt cctgtcgggc gtgccaagaa tttgattgaa
    22141 aaccgttttt atggtggctg tagctcagtt ggtagagccc cggattgtga ttccggttgt
    22201 cgtgggttcg agccccatca gccaccccag ataaaggccc gtacttttcg gtacgggtct
    22261 tttcctgttt gcgcgtactg tccgcgccgc cggctttgcc tttcgcatat gcttctgtat
    22321 catgtgggtt tttctgtctt ttatggtttt tgtcggaggc tttgatggtt gacgcttggt
    22381 ctgtccctga ttttgaatcg aaaatctgtc cgcccgaggc gttggcggcg cgtttggcgt
    22441 tgttgccgcg cccgctggtg tttaccaacg gctgtttcga catcctccac agggggcacg
    22501 ttacttatct ggcgcaggcg cgttcggcag gggcggcgtt ggtgctggcg ttgaataccg
    22561 atgcttcggt gcggcgtttg ggcaagggcg gcgaccgccc ggttaatcct ttggagaacc
    22621 gtgccgccgt tgccgccgcg ttggaaagtg tggatttggt tacttggttt gacgaggata
    22681 cgccggcggc gttgattgag gcggtcaagc cggaggtgtt ggtcaagggc ggcgattggg
    22741 tcgtggataa gattgtcggt gcggcggaaa cgctggcacg cggcggtcag gtgttttcaa
    22801 ttccgtttct gcatcagact tcgacaacga agactttggc aaaaatccgt gcggcagagg
    22861 gcggaaaatg acggttttga agccttcgca ttggcgggtg ttggcggagc ttgccgacgg
    22921 tttgccgcaa cacgtatcgc aattggcgcg tgaggcggac atgaagccgc agcagctcaa
    22981 cggtttttgg cagcagatgc cggcgcatat acgcgggctg ttgcgccaac acgacggcta
    23041 ttggcggctg gtgcgcccct tggcggtttt cgatgccgaa ggtttgcgcg atctggggga
    23101 aaggtcgggt tttcagacgg cattgaagca cgagtgcgcg tccagcaacg acgagatact
    23161 ggaattggcg cggattgcgc cggacaaggc gcacaaaacc atatgcgtga cccacctgca
    23221 aagtaagggc agggggcggc aggggcggaa gtggtcgcac cgtttgggcg agtgcctgat
    23281 gttcagtttc ggctgggcgt ttgaccggcc gcagtatgag ttgggttcgc tgtcgcctgt
    23341 tgcggcactt gcgtgccggc gcgctttggg gtgtttgggt ttggaaacgc aaatcaagtg
    23401 gccaaacgat ttggtcgtcg gacgcgacaa attgggcggc attctgattg aaacagtcag
    23461 ggcgggcggt aaaacggttg ccgtggtcgg tatcggcatc aatttcgtgc tgcccaagga
    23521 agtggaaaac gccgcttccg tgcagtcgct gtttcagacg gcatcgcggc ggggcaatgc
    23581 cgatgccgcc gtattgctgg aaacattgct tgcggaactg ggcgcggtgt tggaacaata
    23641 tgcggaaaaa gggttcgcgc catttttaaa tgagtatgaa acggccaacc gcgaccacgg
    23701 caaggcggta ttgctgttgc gcgacggcga aaccgtgtgc gaaggcacgg ttaaaggcgt
    23761 ggacggacga ggcgttctgc acttggaaac ggcagagggc gaacagacgg tcgtcagcgg
    23821 cgaaatcagc ctgcggcccg acgacaggtc ggtttccgtg ccgaagcggc gggattcgga
    23881 acgttttttg ctgttggacg gcgggaacag ccggctcaag tgggcgtggg tggaaaacgg
    23941 cacgttcgca accgtcggca gcgcgccgta ccgcgatttg tcgcctttgg gcgcggagtg
    24001 ggcggaaaag gcggatggaa atgtccgcat cgtcggttgc gccgtgtgcg gagaatccaa
    24061 aaaggcacaa gtgaaggaac agctcgcccg aaaaatcgag tggctgccgt cttccgcaca
    24121 ggctttgggc atacgcaacc actaccgcca ccccgaagaa cacggttccg accgttggtt
    24181 caacgccttg ggcagccgcc gcttcagccg caacgcctgc gtcgtcgtca gttgcggcac
    24241 ggcggtaacg gttgacgcgc tcaccgatga cggacattat ctcggcggaa ccatcatgcc
    24301 cggcttccac ctgatgaaag aatcgctcgc cgtccgaacc gccaacctca accgccccgc
    24361 cggcaaacgt taccctttcc cgaccacaac gggcaacgcc gtcgcaagcg gcatgatgga
    24421 cgcggtttgc ggctcgataa tgatgatgca cggccgtttg aaagaaaaaa acggcgcggg
    24481 caagcctgtc gatgtcatca ttaccggcgg cggcgcggcg aaagtcgccg aagccctgcc
    24541 gcctgcattt ttggcggaaa ataccgtgcg cgtggcggac aacctcgtca tccacgggct
    24601 gctgaacctg attgccgccg aaggcgggga atcggaacac gcttaaaaaa tatgcctttt
    24661 ttcaacacgg ggcggcatcc gtccgaatct gtccggattc tgccgcccga cagactgccg
    24721 cccgggcggg cggggtttca aacattgagg aaattctaat gaaatggctc ttcacccttt
    24781 tggcggtact caatattgcc gtattcggcg gcacggtggg ctataaattg gccgttaaag
    24841 cggcgggcgg cgtgccggaa aaccgggccg tcgaaaacac gccccccgca acgcccgccg
    24901 ccggcaatgc ggcggcatcc gtggaggata cggcggcact gctcaaaccg ggcgacatcc
    24961 tgagcgaaga acaggcggag cagttgcgct tgaaaaaaga agcggaacag aaaaaactga
    25021 gggagaaaaa acagcgtgaa gaaaaagccc gccgcgaaaa actcgccgcc gaaaaggcgc
    25081 aggcggaacg tgaaaacggc gcggcggatg ccttatgcgc cgcgcaggca agcctcacga
    25141 tggacgaaga cgactaccac cgcatcaaag ggcttttggg caaatggtcg cacgttgcca
    25201 gcaggagcgt cgaaaaacgc accgccaaag ccaaacctgc cgacaaaacc taccgcgtcg
    25261 tcctgcccgt ttccgccgat gccgaaaatc aggcggcgga gctgtctgcc aaaggtttca
    25321 accccatacc gtttgacggc gcattgagtt tgggtgtcgg caacagccgg gaaaacgccc
    25381 aagccctgca aaaccgactt gccgatgccg gattcggcgg ggcgcatatt gtcgaacact
    25441 ttgccgaagc cgacaggcag gacgattctt tgagcgtgtc gcgtatgacg gttttgttta
    25501 cgggcgtgaa tgccgccgat gcggacgaaa tccgtaaaat cacgtcccta tacggcaaac
    25561 tgaacctcaa gtcttgcaaa taagtgcaga ccaaaaacgc caaacccacc gctagggtgg
    25621 gtttggcgtt tcggttttga gtatctgatt aaaaagggaa tgtatgtctc gtatatgccg
    25681 actccaagtg tgaaagtgaa ttgtgaggga atacagtgtt taatggttcg gaacggacgg
    25741 cttccgagtg gttttgatta tacgctaata ataataatta tcaataaaaa tttaaaaata
    25801 aatgcggatt ttttgtaatg cgatgaaaaa taaacaaatt aatctgtccg ccgatgtttg
    25861 tgatttgggt cggcaaactt tcattgcgcc ggacaaatcg gggtcggcag gcatggacgg
    25921 gggcttcgtg cctgacggaa ataaggttgc ccgtgtttga acaaccgtcc gatttggtgc
    25981 ggacagggtt gccattcggg ggtgtttgag cgcggattcg gggatgcaaa agcggtgaaa
    26041 ccgttgcgga tgaaggctta cggatgcccc gaggtatttt taaatgtatt caaacgacaa
    26101 aactcccttc tattgccgta cttagggctt gcgcagcagc ctgcgcgccg gggagtccgg
    26161 gtgtttcgtt ggttgcggtg tcggggcggg cggcaaggtt ttccggccgg tttccctgtt
    26221 cctcacgaca aagccgtgat gccggggaat ggcggtttgc ccgtttgggg ccgcgatgcc
    26281 gtctgaagcc ttcagacggc atttttgccg cctttttcaa tattccgccg aatgccaaaa
    26341 cggttgtccg actgtcggcg tgctggcttg ggctttggtt cgcgcttcga ccggcccggc
    26401 ttcaaatgcc agccgtccgg attcgacggc gagtgcgaag gcgcgcgcca tgttgacggg
    26461 gtcgccgctg cgggaaacgg cggtgtttaa caatacgccg tcaaaacccc attccatcac
    26521 ttgtgccgct tgggaaggca aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag
    26581 gcgttcgcgc aggattttga gcgcataggc gtgaaccgcc cccaaacccg tgccgatggg
    26641 agccgcccac ggcatcaacg cctgacagcc cgcatcgagc aggcggcggc aggcaatcag
    26701 gtcttcggtg caataaggca gcactttgaa gccgtcttta atcaggattt ccgccgcttc
    26761 gacgagttgg aacacgtccg gctgcaaggt gtcgtcgtcg ccgatgagtt ccaattttat
    26821 ccaatcggtt tcaaacactt cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct
    26881 ttggcagcct gccgtgttcg gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa
    26941 cccctgaccg tgcgcctcgc cgccgcatcc cgtgcggcgc agcgagacgg taatcatcgc
    27001 gggccgggcg gttcggacgg attgtttgag gatttcaggg gtcgggtagg cggccgtgcc
    27061 gagcagcagc cgcgaaggga aagtttcgcc gtacagggtg agcatgatgg attctttggt
    27121 atggttgtcg gacgcgatgc cgtctgaaag ccggcggcgt gcctttgggg tttcagacgg
    27181 cattcagccg ccgacgacgg ggcggacgat gtcgattttg tcgttttcgt gtaaaacggt
    27241 ttccgcatac gcgcctttgg ggatgaaaac ggtgttgacc gccacggcaa agggcttttg
    27301 cggcgcggtt tgggcgatga ggtcggcaac gcttgtgccg tgaagttcgg cgggtccgcc
    27361 gtttaagatg atgttcatgg tttctcgatg tttctgtatt tatagcggat taacaaaaac
    27421 cggtacggcg ttgccccgcc ccggctcaaa gggaacgatt ccctaaggtg cccaagcacc
    27481 gggcgaaccg gttccgtact atttgtactg tctgcggctt cgccgccttg tcctgatttt
    27541 tgttaatcca ctatatctag ccgaattact ttattttttg atacgcaacc ggccggttgc
    27601 cgtcattccc gcgcaggcgg gaatccattt ttgaaattcg gaaactgttt ttcaaatgat
    27661 ggtttctcaa gttttacggc ggattcccgc ctgagcggga atgacggaat ttgatgatat
    27721 gccacattta aagttaagca tatttgcgat aacgggctaa gttttgccta cacggcaatt
    27781 gaattttctt ctttcggttt tatccatccc acaaagcctg aaacgcttta accaccgctt
    27841 cgggatttgc cgcttcggtt acggcgcgga cggcggcgag tgaggaaacg ccggtggcca
    27901 gcacggctcg ggcgttgttc aaatcgatgc cgccgatggc gacgacgggc gtgccgcgag
    27961 cctgttccac atattcgcgc aatttgtcca agccttgcgg ggcggtgggc atttgtttgg
    28021 tcgtggtctg gaaaatcgcg ccgctggcga tgtagccggg gtgtacggac agggcgcggt
    28081 cgagttcggc gacggagtgc gtactcaaac ccaaacgcaa accggcggcg gcgatggcgg
    28141 caaggtcggc ggtgtccatg tcttcctgcc cgagatgtac gccgtatgcg cccgcttcga
    28201 ttgcctcgcg ccagtggtcg ttgatgaaaa gctgtgtgcg gctgccttga caggcggcga
    28261 cgcagcgggc gatttcgcgt ttcaattcgt tgccgtgcaa ggttttacag cgcagttgca
    28321 ccgtgtcggc acctgctttg accatgcgcc ccacccaatc ggcggtggga acgacggcgt
    28381 agaatttaag tagggatttt aggggcggga aggtcatagg gtgtcctttc gggcggcttt
    28441 aatcttgtct tccgatatac gccaaaccgc tttcttcatc acgttcgggc gcgtcttttc
    28501 cgtcaaacag tgccactgcc aatctgacgg cggcggcggt tacggcgggg gaaatcataa
    28561 agccgtgccg gaaaaggccg ttgatttcga tgaggcggcg ttcgcggctg tagcggattt
    28621 cggggttgtg gtggttgagc gtggggcgca ggccggcggc gatttcgagg atgtcggctt
    28681 cgccgaaggc ggggtggacg gcatagagcg cggataagag ttccagcccg gaacgtacgc
    28741 tggcgggggc ttggctttcg ctttcgattt gggtcgcgcc gatgacgaag acgtggtttt
    28801 ctttcggggc gatgtagagc ggatagcgcg ggtgcagcag gcgcacgggg cggttgagcg
    28861 tgatttcggg cgtgtaaacc cgcgccactt cgccgcgtat gccgcgcaag gtgctggtgt
    28921 gctcggggga ttggttccac gcggttttcg cgccgtagcc ccggcagtcg attacccagt
    28981 cgtattgggc ttgcaggtct tggggggcgc attcgtgttc ccaatggcaa gggacgttca
    29041 gttcgtccaa agcgtcggca agtgcagaca atatttgccg cccgtcgagc tggccttcgg
    29101 tcggcaggta gatgccgtct gaaaaacgtc cgccgagttg cggttcgcgt tcggcgattt
    29161 catcggcgcg ccaacggacg atttcgtcat ccgctacgcc gccgcgtttg agatggcgga
    29221 cgaactcgct ggataatggc ttgtcctgcc cgtgccacac aatcaggctg ccgttttcct
    29281 gcatcatcgt gtgcgtgttc agacggcatc ggatgccgcg ccaaagcgga atgctctgcc
    29341 tgcccagcct gatgacttcg ggcgttgcct cgaccgcttc cgccgcaggc gcgagcatcg
    29401 cggcggcaac ataggcggcg gcgtgttcgc cttggcgggt gcctttgtcg aaaagttcaa
    29461 tctgataacc ttgttctgca agctgcaatg cggtcagcct tccggaaagg ccgcctccga
    29521 ggacggcgat acgggtcatg acgggttcct ttgtaaagat tgggtttttt taaagaaaag
    29581 gcgtaccgat acggtggcaa tggcaacggc agacattacg ggggcggtca gaccgatgct
    29641 gctttcccaa ccggacgaga gcaggaagcg gtagaggatg aagcctgcca gccacagaac
    29701 cagtccggca aagtcaaagc cttcaatctc ctcacgccgt tttaagacga aaaagtcggc
    29761 aatcaaaacc gccgccatcg gcgcaaatac cgagccgata agcagcagga agttttcata
    29821 ttcggtaacg ggcagcatga cggcaagcac cgtgccgatc agggtaacgc cgacagcgac
    29881 gggtgtttcc gcaaaacgcg cggaaatgtt gttcgcactc gcgccggcgg aataggtatc
    29941 gagaaacgtt gtggtaacgg tggagaggac gactgccaga atgcccgtta tgcccaagcc
    30001 cgcgcccaac aggattttcg ccacgtcggt ttctccggta aacagagccg ccgccaaacc
    30061 caaggcatac atccagcagc ccgtcagcgt ataggcgagc gttgccgtca gggttgccgc
    30121 aaacgggcgg cgtgcttggc gcgtgtagtc ggcggccagc ggcagccagg aaagcggcat
    30181 gacggcggac agttcgactg ccgttccgaa ggtcatgccg tctgaaacgg cgggcgcggc
    30241 gtttgtgccg gacgaagcga acacttcgac gctcaaccac aacacggcaa gcagcatcag
    30301 cagcatcgaa acggttttca gcccgcccgt tctgcgtgcg ccgaaaacca gccacagcac
    30361 gatcagtgcg ccgtttgcca atgcccacca gacaaaggat tcgccgtccc acaacacttt
    30421 gcctaaagcg gagctgaccg ttgcgccgac gtaaatcatc accgccgtcc agccggccag
    30481 ttgcagcata ttcgccacgg aaaacagcac tgaaccgcat ttgccgaacg acaggcgcac
    30541 actttccatc gagctgcgtc cggtcagtgc gccgatatac gccgccgcaa aaaacagcgc
    30601 gccgccgacg gcatgaccca aaagcagggc cgccagaccg cgctgccagc ccaagggggc
    30661 gagcagcgta cccgtgctga tttcggcaat cgataccgcc gcgccgaacc aaaccagccc
    30721 gatggcggcg gaagatgaag gagaggaggc attgcccgac atatgacgtc ctttaaaaag
    30781 aaggaaggtg gtcggggaag tgcggaaaag ggaacggaag cccgaaaggg cggggaggtt
    30841 ttgtttccta cgcaggcatt accccgacag gttctacgga ttttgctttc gcaatctcag
    30901 ccgcccggcg ggcggcactc cgacaagaag gctttaagtt tacacaaagc aacgtgcgtt
    30961 ggcaaaattc ttacattttc gacggcacgg cgcatttcca taacaaatgc cgtataatca
    31021 agcccctgat tttttatgcg ccgacactat gcatccgacc tattccgccg tacaggcgcg
    31081 gctgctcgaa gccaaccgcc tttcccccga actgttcgcc aaaagcctgt gcatcatcgg
    31141 tgcgcaccac gtcgattatg ccgatatcta ctgccagcgc accgcttatg aaagctggca
    31201 tttggaagag ggcatcgtca aatcgggcag cttccaaatc gatcagggtg tgggcgtgcg
    31261 cgccgtttcg ggcgacaaaa ccgcctttgc ctacgccgac agcctgtgca tcgattcgat
    31321 aaaccgttcc gcccgcgccg tccgcgcgat tggggcggca ggcggcaagg tgtccgccaa
    31381 aatgccgtct gaaacgcgcg gcaagccggt ttgttccgca tccgacccca ttgccggcct
    31441 cgattccgcc gccaaagtcg cgctgttgaa caaagtggaa gcaatcgcca aagccgccga
    31501 tccgcgcatc gtgcaagtga tggccggttt gacctgcgaa tacgatatgg tttacctcgc
    31561 ccgtctggac ggcaaacacg ccgccgacat ccgcccgatg gtgcgcctga acgttaccgt
    31621 catcgccaaa cagggcgaac gccgcgaaca gggcggcgcg ggcggcggag gacgctacga
    31681 cttggcttat ttcgatgaaa ccttggtgcg gcagtttgtc gatgccgccg tcaaacaagc
    31741 cctgaccaat ctcgaatccc gtcctgcgcc tgccggcgag atgaccgtcg ttttgggcaa
    31801 cggttggccg ggcgtgttgc tgcacgaagc ggtcggacac ggtttggaag gcgatttcaa
    31861 ccgcaagggg accagcgtct tttccggccg gatcggcgaa cgcgtcgccg ccaaaggcgt
    31921 taccgtcgtc gatcagggcg atattgccgg ccggcgcggt tcgctcaaca tcgacgacga
    31981 aggcaacgaa acacgccgca ccgtattgat tgaagacggc atcttggtcg gctatatgca
    32041 ggacgaaacc aacgcccgcc tgacgggtac gcaatcgacc ggcaacggcc gccgccaaag
    32101 ctacgcctcc gtcccgatgc cgcgtatgac caacaccttt atggaaaacg gcagctacga
    32161 gccggaagaa atcatcgcgt ccatcgacaa gggcatttac gccgtcaact tcggcggcgg
    32221 acaggtggac atcaccagcg ggaaatttgt cttcggcgcg tccgaagcgt ggtgggtgga
    32281 aggcggcagg ctgcaatacc ccgtcaaagg cgcgaccatt atcggcaacg gccccgaagt
    32341 gctgaaacac gtttcgatga taggcaacga taccgcgctc gacagcggtg tcggcgtgtg
    32401 cggcaaagaa gggcagagcg tccccgtcgg cgtggggcag ccgaccctgc gtatcgatgc
    32461 cgggctgacc gtcggcggca gcgcaatctg acgcggaatc cggcacgatg ccgtctgaaa
    32521 ggttttcaga cggcatcgcc ttattctttt ttccgatgtt tgatattcct aagcattctt
    32581 taacattatc ttacaagttc attaaaatac cgcctttcgt taagctgatt tctgatgaac
    32641 tggccttatt taatcgatgc cgtacccaaa ttcgccgatg cggcaaagct gacgctggaa
    32701 ttgtccgttt acggcgttgt tttgtcgttg ctgttcggcc tgcccgtcgc cgtggtaacg
    32761 gcataccgca tccgcccctt ctacgcattg gcgcgcgcct atatcgagct gtcgcgcaat
    32821 acgcccctgc tgatccaatt gtttttccta tattacggtc tgccgaaaat gggcatcaaa
    32881 tgggacggtt tcacctgcgg cgtaatcgcg ctggttttct tgggcgcaag ctatatggcg
    32941 gaagctgtcc gcgccggcat cctcgccgtc cccaaagggc aggtcggagc gggcaaggca
    33001 atcggtttga gccgctttca agtgttccgc tatgtcgaat tgccgcaggt ttgggcggtc
    33061 gccgttcccg ccatcggcgc aaatatattg tttttaatga aagaaacatc cgtcgtcagc
    33121 acggtcggca ttgcggaatt gttatttgtt accaaagacg tcatcggtat ggactacaaa
    33181 accaatgaag ccttgttcct gctgtttgcc gcttatttga ttatcctgct gcctgtttct
    33241 ttgttggcgc ggcggattga aaaccgcgta cggagtgcga aatatggcgt ttgaatggct
    33301 gtttgaagga aaaaatgcgg cacggttggg cgaaggcctg ctcttaacgg cacaaatttc
    33361 tttaatctct gttgcggctt cttgcgtatt gggcacgctg ttcggcttgg ttttgcgttc
    33421 gcgcaaccgg cttgtccgct ttgtcggacg gttttatctc gaaaccatcc gaatcgtgcc
    33481 gattttggtg tggctgttcg gcctgtattt cggcctatcc gtctggacag gcatccatat
    33541 cggcggattt tgggtctgcg tctgggtatt ttccctgtgg ggcgttgccg aaatgggcga
    33601 cttggtgcgc ggcgcactgg aatcgattga aaaacaccag gtcgaatcgg gtcttgcgcc
    33661 cggcttgagc cgggggcagg tattccgctg catcgagctg ccgcaaagca tccgccgcgt
    33721 attgcccggc gcggtcaacc tgttcacgcg catgatcaaa accagctcgc tcgcctggct
    33781 tatcggcgtg atcgaagtcg tcaaagtcgg gcagcaaatt attgaaaact cgttgctgac
    33841 gcagcccaat gcttcatttt gggtttacgg cctgattttt atgctgtatt tcttctgttg
    33901 ctggccgcta tctctactgg cggcaaaact tgaacaaaaa tgggaacact gacatggctt
    33961 tactgagcat ccgcaagctg cacaaacaat acggcagcgt aaccgccatc caatccttag
    34021 acttggactt ggaaaaaggc gaagtcatcg tactgctggg cccgtccggc tgcggcaaat
    34081 ccaccctcct gcgctgcgtc aacggtttgg agccgcacca aggcggcagc atcgtgatgg
    34141 acggtgtcgg cgaattcggc aaagacgttt cctggcaaac cgcccggcaa aaagtcggta
    34201 tggtctttca aagctacgaa ctgtttgccc acatgaccgt catcgaaaac atcctcttag
    34261 gcccggtaaa ggtacaaaac cgcgaccgtg ccgaagcaga ggcgcaagcc ggcaaactgt
    34321 tggaacgcgt cggactgcta gaccgcaaaa acgcctatcc gcgcgaactt tccggcggtc
    34381 agaaacagcg catcgccatt gtccgcgccc tgtgcctgaa tccggaagtc atcctgctgg
    34441 acgaaatcac cgccgcactt gaccccgaaa tggtgcgcga agtcttggaa gtggttttgg
    34501 aactcgcccg cgaagggatg agtatgctca tcgtaaccca cgaaatgggg ttcgcacgca
    34561 aagttgccga ccgcatcgtc tttatggaca aaggcggcat cgtcgaatcg tccgaccccg
    34621 aaaccttttt ttccgcacca aaaagcgaac gcgcccgcca atttctggca ggtatggact
    34681 actgatttac cgcaaaccgt ccgaaccctt ttcagacggc atttcatagg gcagtgcttt
    34741 atgtgtcgtc ctgtgtgttg aaacatccca agcaaacatt caaaaaggag atttccaatg
    34801 aaattgaacg ccaaactcaa agcccttttg gcttccgccg ccatcgccgt cggtctgacc
    34861 gcctgcgggg gcggctccgg cgatgcccaa tcttcacaaa gcagcggtgc ggcaaccgtt
    34921 gccgccatca aagaaaaagg cgttatccgc atcggcgtat tcggcgacaa acccccgttc
    34981 ggctatgttg acgcaaacgg caaaaaccaa ggctttgacg ttgaaatcgc caaagacctg
    35041 gccaaagacc tgctcggcag ccccgacaaa gtcgaattcg tcctgaccga ggccgcaaac
    35101 cgcgtcgaat acgtccgttc cggcaaagtc gacctcatcc tcgccaactt tacccaaacc
    35161 cccgaacgcg ccgaagccgt cgatttcgcc gatccttaca tgaaagtggc gttgggcgtg
    35221 gtttccccca aaaacaaacc gattaccgac atggcgcaat tgaaagacca aaccctgctg
    35281 gtcaacaaag gcaccaccgc cgacgctttc ttcaccaaaa gccatcccga agtcaagctg
    35341 ttgaaattcg accaaaacac tgaaaccttc gacgcgctga aagacggtcg cggcgtagca
    35401 ctcgcgcacg acaacgccct gctgtgggcg tgggcgaaag aaaacccgaa ctttgaagtc
    35461 gccatcggca atctcggtcc tgccgaattt atcgcccccg ccgttcaaaa aggcaacgcc
    35521 gacctcctga actgggtcaa cggcgaaatc gccgccatga aaaaagacgg ccgtctgaaa
    35581 gccgcctatg aaaaaaccct cttgcccgtg tatggcgaga aagtcaaacc ggaagcattg
    35641 ttggccgaat aagtttgttt gacaagaaaa tccgtatccg catttgaagg cgggtgcgga
    35701 tttttttcag ccgccgaaaa tattgcaaat tcaaaggaaa aatcaactat ctcatcgttt
    35761 tatttatttt attattcttg cgcgtgcgga aatcaagttc gtccggtttt gcctgttttt
    35821 gaatttcggg aaacttccaa atcgtcattc cagataaaac gcttcagcct tatgtttccg
    35881 gatgaccgtt ttcgcgggat gacggcggag cgtttctgtt attcggataa attcctacgg
    35941 tattgaaacg gcgataaggt gttttccttt attatataat ttcaatgcaa aatgatattt
    36001 tttgaacaag cgtgccgcaa agccggaaaa aatgttcaag ccgcatcggc ggcagacggg
    36061 acgaaaaatg acgtttgacg aatggttggg cttgtcaaaa ctgcctaaaa ttgaagcaag
    36121 gatgctgctg caatatgttt cggaatatac gcgcgtgcag ttgttgacgc ggggcgggga
    36181 agaaatgccg gacgaaatcc gacagcgggc ggacaggctg gcgcaacgcc gtctgaacgg
    36241 cgagccggtt gcctatattt taggtgtgcg cgaattttat ggcagacgct ttacagtcaa
    36301 cccgaacgtg ctgattccgc gccccgaaac cgaacacttg gtcgaagccg tattggcgcg
    36361 cctgcccgaa aacgggcgcg tgtgggattt ggggacgggc agcggcgcag ttgccgtaac
    36421 cgtcgcgctc gaacgccccg atgcgtttgt gcgcgcatcc gacatcagca cgcccgccct
    36481 tgaaacggcg cggaaaaatg cggcggattt gggcgcgcgg gtcgaatttg cacacggttc
    36541 gtggttcgac accgatatgc cgtctgaaag gcaatgggac atcatcgtgt ccaacccgcc
    36601 ctatatcgaa aacggcgata aacatttgtc gcaaggcgat ttgcggtttg agccgcaaat
    36661 cgcgctgacc gacttttcag acggcctaag ctgcatccgc accttggcgc aaggcgcgcc
    36721 cgaccgtttg gcggaaggcg gttttttgtt gttggaacac ggtttcgatc agggcgcggc
    36781 ggtgcgcggc gtgttggcgg agaatggttt ttcgggagtg gaaatcctgc cggatttggc
    36841 cggtttggac agggttacgc tggggaagta tatgaagcat ttgaaataat tgtttgcaaa
    36901 attggcggat tttccgttat tgggttatga tgtgttttga tatgtaaaca tttataggtt
    36961 tggagcgata aacaatgaat gcagtcgtag ttgccgtaat cgttatgctg gtgctgtcgc
    37021 tgtcgcgcgt gcacgtggta ttgagcctga cggtcggcgc gtttgtcggc ggcgcggtgg
    37081 cgggtatgcc gctgcaaaac attgccgatg cggcgggaca ggtcagtcag gcggggatta
    37141 tccccgtgtt caacaaaggt ttggaaggcg gcgcgaagat tgcgctttct tatgcgatgc
    37201 ttggtgcgtt tgcgatggcg attacccatt ccggcctgcc gcagcagctt gccggcgcgg
    37261 tcgtccgcaa gctgaaccgg ggcggtatgc ccgacagcgt gcgttcgggc gagggcgcgg
    37321 tcaaatggct gctgctttcc atcatccttg tgatgggcat tatgagtcag aacgtcatac
    37381 ccatccacat cgcctttatc ccgatgattg ttccgccgct gcttttggtg tttaaccgcc
    37441 tgaaaatcga ccgccgcctg attgcgtgcg tcatcacttt cgggctggtt acgacttata
    37501 tgttcctgcc ttacggcttc ggcgcgattt ttttgaacga aatcctgttg ggcaacatcc
    37561 attccgccgc gccgcagctt gatgtgaaaa acatcaacgt gatggcggca atggcgattc
    37621 ccgcattggg aatgctggcc ggactcctgc tggcgtttgt ccactaccgc aaaccgcgac
    37681 tgtaccaaag caacaatgcc gatacggcgg gcaacgccga tgcggcaaac cgtccgcagc
    37741 cgtccgccta ccgcagcctg gttgccgccg ccgccatcgc cgtatgcttt gccatacagt
    37801 tgatgtatga agattcgctg gtgttgggcg cgatgctcgg tttcgccgta tttatgatgt
    37861 tgggggtcat taaccgcgac aaggcaaacg acgtattcgg cgaaggcatc aagatgatgg
    37921 cgatggtcgg cttcattatg attgccgcgc agggttttgc cgccgtgatg aatgcgaccg
    37981 ggcatattca gccgctggtg gaaagcagta tggcgatatt cggcaacagc aaaggtatgg
    38041 cggcgttggc gatgctggtg gtggggcttt tggtaacgat gggcatcggt tcgtcctttt
    38101 ccactttgcc gattattgcc gcgatttatg tgccgctgtg taccggtttg ggcttttcgc
    38161 cgcttgccac cgccgccatt gtcggcacgg cgggggcgtt gggcgatgcc ggttcgcccg
    38221 cgtccgattc cacgctgggc ccgacgatgg ggctgaacgc cgacgggcgg cacgaccata
    38281 tccgcgattc cgtcatcccg accttcatcc actacaacat cccgcttctg attgccggct
    38341 ggattgccgc gatggtgctg taaatggacg cggttcaaga gttggaacgc cgtattgtcg
    38401 aactggaaat ccaaaccgcg cttcaggagg acgtaatctc cggtctgaac gcgatggtgg
    38461 cggaattgcg gcagacgttg gatttgcagc aggctcagtt gaggctgctg tatcaaaaaa
    38521 tgcaggacag gaatcccgac gcgcaagagc cgtattccct gcgcgacgag attccgccgc
    38581 attattgatg cgccgccgta tccggatttc cttaaataaa ggctgtgttt gaatattccg
    38641 ccggtgcaac cctaagatgt aaccttaatc agtcggacaa aatgcctttt atccgatgaa
    38701 accggttctt gtccgaacgg cgatgagcgg cttcccccgc tgcaaacaga taaaaccaac
    38761 cgggtattca aacacagcca aaaaaagccg tccgaaccca aaggggacag acggccaaac
    38821 acattacggg gaaaacgtct tactcaatga gtctgcgaaa cagacattgc taaaacaact
    38881 gcaattataa ggtaagttta tttttttgca aagaaatgta aatttttaaa tttttaaaac
    38941 cgttccgccg catccgttcg gatgccgtct gaaacggcgg cagggtttta ccgtgccgcg
    39001 ccgcaaaccg ggcattcggg gttgcgcggc aggtcgaaat attgccagcc cccttccaag
    39061 gcacggtaaa ccgccagcct gccgtgcgac ggttcgcccg cttccagcag gattttcaga
    39121 gcctccgccg cctgtgtgca gccgacgatg ccgaccagcg gcgagagcac gccgaagagg
    39181 gaacagatgc cgtctgaagc cgatccgccg tcgaacaggc aggcgtaaca cggcgagtcg
    39241 ggcaagtcgg gacggtatgc ggcaagctgt ccttcaaaac gtaccgccgc ccctgaaacc
    39301 agcggtgttt tcgtttgcac gcaggcacgg ttgaccgcct gccgcgtggc gtagttgtcg
    39361 cagcagtcta aaacgatgtc ggcggtttga accaaaccgg tcaggcggca gccgtcgagt
    39421 ttttcgttga cggcgcggac atcgacagta tggttgatgc gtttcaggcg gcctgccaag
    39481 gtttcggctt taggcttgcc gacatcgccc tcgtcaaatg cgacttggcg ttgcaggttg
    39541 tgcagctcga ccgtgtcgga atcggctatg gtcagcgtgc cgacgcccga ggcggcgaga
    39601 tagggcaggg cggcggcgcc caatccgccg cagccgacga ccaaaatatg cgcggcggaa
    39661 agcttctgct gcccttcgat gccgatttcg tccaagagga tgtggcggct gtaccgcagc
    39721 aggagtgcgt cgtcgttgtc gtgttcggtc gcggtcatga tgatgttcgg aaaaaacagt
    39781 tgcgggcgat tgtaacgccg ccgtcgggcg gcgttcaact tcagacggca tttcgggaca
    39841 tgagtggtta aagtgtgaac ggtttggcac ggatgcggca tttgaggtac atttacagta
    39901 tttacggcaa tgagaggaaa atcatgcaac tgcatatcct gaacaatccg aaggacgcgg
    39961 ctttggcggc ggacgcggaa tttttgaaac agtccctgtt caacctcctg cacgaagaag
    40021 cctcgccgtt ggttgtcgaa acggtcaaac tcttgtccac ttccgacgac agcgcggcat
    40081 tgattgaaaa agtattgccg caattggacg aacggcagac ctacgatctg acgcttgcct
    40141 gcgggctgtt cgcccagatt ctgaatatcg ccgaagacgt gcaccacgaa cgccgccgcc
    40201 aaatccacga agatgccggc cacaacgcag cagaaggcag cctgacggaa accgtccgca
    40261 ggctcaaagc ggggaaagcc gacggcaaat cggtgcagcg gcagttggac aatacgtccg
    40321 ttaccgccgt tttgaccgcg cacccgaccg aagtgcaacg ccaaaccgtt ttgaatttca
    40381 accgccgcat ccgcgcgctg ctgccgcaac gcgaacgctg caccaatgcc gacgcgctgg
    40441 cacggctgcg ccgcgaaatc gacaccgtgc ttctgggctt gtggcagacc agcgaaacgc
    40501 gccgccacaa actcagcgtc aacgacgaaa tcaacaacgg cgtgtccatc ttcccgatga
    40561 gcttttttga agccctgccc aaactctacc gcaagatgga acacgacttt cagacggcct
    40621 atcccgatgt ccgcgttccg aacatcctta aaatcggcgg ctggatcggc ggcgaccgcg
    40681 acggcaatcc gtttgtttct ggcgaaaccc tgcgctttgc cttccgccgc cacgccgatg
    40741 ccgtgttccg tttttaccgc agcgaactcg acaaacttta ccgcgaactg ccgctttcca
    40801 tccgccgcgt caaagtcaac gacgacgtaa tggcactggc agccctctcg cctgacgaag
    40861 aaatcgcccg caccgaagaa ccttaccgcc gcgccattgc ctacattatg gcgcgcgcta
    40921 tgggcaaagc gcgctcgctc ggtttgggca tgggctgcaa attcggcttt ctcgagcctt
    40981 atgcttcggc ccaaaaattt ctcgatgatt tgaaaaaatt gcaacgttcc cttatcgaca
    41041 acggcagccg cctgcttgcc gaaggccgtt tggcagacct catccgttcc gtgtccgtgt
    41101 tcggcttcca catgatgccg ctcgacctgc gccaacacgc aggcaaacac gccgatgtgg
    41161 ttgccgagct tttccaacac gcaggcttgg aagactacaa cagcctgaac gaagagcaaa
    41221 aacaagccgc cttgttgcgc gaattgagtc accaacgtcc gctgtacagc ccgttcatca
    41281 catacagcga ccatacccgc cgcgaattgg cgattttcaa cgaagcgcgc aaaatcaaag
    41341 acgaattcgg cgaagatgcc ttaacacaaa gcattatttc caactgcgaa caacccggcg
    41401 acctgctcgc cttggcattg ctgctgaaag aaagcggcct gttggcggtg gaaaacggca
    41461 aaccgcacag ccgcatcaac atcgtgcctc tgtttgaaac catcgaagcg ctcgaaaatg
    41521 cctgtccggt catggaaacc atgttccgcc tcgactggta tgatgccctg ctcgaaagcc
    41581 gcggcaacat ccaagaaatc atgctcggct attccgactc caacaaagac ggcggctacg
    41641 ttaccagctc atggtgtctg catcaggcgg aattgggctt ggtcgaactc ttcaaaaaat
    41701 acgatgtccg tatgcgcctg ttccacggtc gaggcggcag cgtcggacgc ggcggcggcc
    41761 cgtcttacca agccatcttg gcgcaacccg caggcagcgt tgccggacaa atccgcatta
    41821 ccgagcaagg cgaagtcatc accgccaaat acgccgaccc cggcaacgcc caacgcaact
    41881 tggaaacctt ggttgccgcg actttggaag ccagcatcct gccggataaa aaagaccctg
    41941 atgcgaaact gatgcaggca ttgtcggacg tatcgttcaa atactaccgc gaactgatta
    42001 cccaccccga cttcatcgac tacttcctgc aaaccagccc gattcaggaa atcgccaccc
    42061 tcaacctcgg cagccgtccc gccagccgca aaaccctggc gcgaattcag gatttacgcg
    42121 cgattccgtg ggtattctcc tggatgcaaa accgcctcat gctgccggct tggtacggtt
    42181 tcggcagcgc ggtggaaacc ttgtgcgaag gcagccccga aaccctcgcc gccctgcgcg
    42241 gacacgccca aaacaacccc ttcttccaag cgatgctctc caatatggaa caagtgatgg
    42301 cgaaaaccga catcaccttg gcggaaaact acgccggttt gagcgaatca cccgaaaagg
    42361 caaaagtcat cttcggcatg attaaggaag aataccgacg cagccgcaaa gccctgctcg
    42421 acctgctgca aaccgaagaa cttttgcgcg acaaccgcag cctcgcccgt tcgctcgctt
    42481 tgaggattcc atacctgaac gcgctcaacg gtttgcaagt cgccatgctc aaacgcctgc
    42541 gcaaagaacc cgacaatccg cacgccctgc tgatggtgca cctgaccatc aacggcgtgg
    42601 cgcaaggttt gcgcaatacc ggctgatagt gccgcatcgg ggcaaaatgc cgtctgaacg
    42661 cctttcagac ggcatttacc tgaccgcact tgcagagaaa caccgattgt tttaaagtga
    42721 acggcagtga tatgttgaaa gacgaccaat gaaaattacc gttatcggcg caggttcgtg
    42781 gggtacggcg ctcgccctgc atttttccca acacggcaac cgcgtatccc tgtggacgcg
    42841 caacgcagac caagtccgcc aaatgcagga agcgcgtgaa aacaaacgcg gactgcccgg
    42901 cttttccttt cccgaaacct tggaagtgtg tgcggatttg gcagaggcgc tcaaagacag
    42961 cggacttgtc cttatcgtaa cctccgttgc cggattgaga agcagcgcag agctgctcaa
    43021 acagtacggc gcgggacacc tccccgtcct cgccgcctgc aaaggcttcg agcaggatac
    43081 agggctgctg accttccaag tcttgaaaga agtattgccc gacaataaga aaatcggcgt
    43141 actttccggt ccgagttttg cacaggaact cgccaaacaa ctgccctgcg ccgtcgtcct
    43201 cgcctccgaa aaccaagagt ggattgaaga actcgtaccg cagctcaaca cgaccgtcat
    43261 gaggctttac ggcagtaccg acgttatcgg cgtggcagta ggcggttcag tcaaaaacgt
    43321 catggcaatc gctaccggac tctccgacgg actcgaatac ggactcaacg cccgtgccgc
    43381 actggttacg cgcggactgg ccgaaatcac ccgccttgcc tccgcaatgg gcgcgcagcc
    43441 caaaaccatg atggggcttg ccggcatcgg cgatctcatc ttgacctgta ccggtgcact
    43501 ttcgcgcaac cgccgcgtcg gcttgggttt ggcagaaggc aaggaactgc atcaggtgct
    43561 ggtcgaaatc gggcacgttt ccgaaggggt cagcaccata gaagaagtct tcaataccgc
    43621 ctgcaaatac caaatcgata tgccgattac ccaaaccctg ctgcaactca tccgcaaaga
    43681 aatgacccct cagcaggtcg tcgaaaggct gatggaacgc agcgcgcgtt tcgaataaac
    43741 aacaaacaga tgccgtctga acccccagac ggcatacaga caggtaaggt catgaaacaa
    43801 aatatcgaaa aactcgaaag cagcgtttat acgttggtac aaaaattcga aaccctcgtc
    43861 agcgaaaacc gccgcctcaa agaaaccgtc gccgaactcg aacgggcgca cgagcggcaa
    43921 aaactcgaac acgagaccgc cgtcgacgaa ctcagcgaag ccctgctcgt ccaagtcggc
    43981 aaactcaaag aagacctgca aaacaaaatc gacagcctga cggaagaaaa tgcacgatac
    44041 cgcgccctgc tcgaacagag cagggagaaa atcagcgcac tggcagcgcg cctcccccaa
    44101 cggcaggaaa cgcagcaata aggattaaag gatgaacatc gaacaagtct acatcgaagt
    44161 catgcacgcc cggctgaccg tcaacacgcc ggcagaagaa aaagacacac tgttgcaggc
    44221 agtcggaatg ctcaacggca aagccgaagc catccgcgaa ggcggccgcg tcgcggacag
    44281 cgaaaaaatc gtcattatgg ccgcgctcaa cgtcgtccac gacctattga aaacctccct
    44341 gaacggcggc gatttggcaa tcggcgattt tgcgcgtaaa ataaccgata tggacaacgc
    44401 ctgccaaaag gcactatccc gcttggggca ggaataaccc ctttttccct gcggtgtccg
    44461 cgaaggcata tattcctttg aaccaataag ttcgcttaag gttgccggga gcagtagtgg
    44521 gcgcgagcgt ccttttgcgg acgcacccga aactacccga ggcagccgcc ttgtcagcaa
    44581 ggttcaagcg gattcggccg aaacggctct cgcggggatt cccattcaaa accgcatcgc
    44641 aacgatgcgg tttttcacat tggccgccgc cccgcccgaa aaagcagtat gcagtctgcc
    44701 gaaatccgga aaatccttcc gtaacgcggt aatgcgattg acaaaatcaa atcctgtctt
    44761 taaaatccat aactttctat atctatctgg atttccacta tgaaaacctt ttcagcgaaa
    44821 cctcacgagg tgaagcgcga atggttcgtc atcgatgccc aagacaaagt cttgggtcgc
    44881 gttgcaaccg aagtcgccag ccgtctgcgt ggcaaacaca aacctgaata caccccccac
    44941 gtcgataccg gcgattacat catcgtcatc aatgcggaca aactgcgtgt aaccggtgcc
    45001 aaattcgaag ataaaaaata cttccgccat tccggttttc caggcggcat ctacgagcgc
    45061 actttccgcg aaatgcaaga tcaattcccg ggccgcgctt tggagcaggc tgtaaaaggt
    45121 atgctgccca aaggtccgct gggttacgcc atgattaaaa aactgaaagt gtacgctggt
    45181 gcggagcatg cccatgctgc gcaacaaccc aaagttttgg aactgaaata ataaggacac
    45241 gacatgaacg gtaaatacta ctacggcaca ggccgccgca aaagttcagt ggctcgtgta
    45301 ttcctgacta aaggtacggg tcaaatcatc gtaaacggcc gtcccgttga cgaattcttc
    45361 gcacgcgaaa ccagccgtat ggttgtccgc caacctctgg ttctgactga aaacgccgaa
    45421 tctctcgaca tcaaagtaaa tgttgtcggc ggcggcgaaa ccggtcagtc cggcgcaatc
    45481 cgccacggta ttacccgtgc cctgattgat tttgacgctg ctttgaaacc cgccttgtct
    45541 caagccggtt tcgttacccg cgatgcgcgc gaagtcgaac gtaaaaaacc gggtctgcgc
    45601 aaagcacgcc gtgcaaaaca attctccaaa cgttgatttt ggaaattcaa aaaaccctgc
    45661 ttatcgcagg gttttttatt tgtaacaggc ggtttcccat tggtaatcta aagattacgg
    45721 attgggcaaa aatcaaaaac agcaccagta tcgactacac ggacgaactg accgtcaggg
    45781 atggctgtta ctggagagga catcgtattt ctactttgta aaataacttt taatttattt
    45841 agagttattg aaaaaataga acggctattt caactagcct ataaaaatgg caatctatta
    45901 gggttagcat aggtgtcacc gccgccgttc aagccgatca gtaaaggaac agtttcctag
    45961 tttttagcta acctgtatta tataatgggg agaatatctt aaataaccaa gcatccttaa
    46021 tcataattta gagaaattat tttttcagag tctaaccctt ttaatatgat taacttatta
    46081 actttatcta tttcccagtc tgatattata tcggtagata ctgaattctc tcttattaaa
    46141 tttttatctg taataattgc acccaactca tgaataatac aaattttttg attatccaat
    46201 aaataaatat caaaaactat tccatctata tttagtatgt taacttttga attttgtata
    46261 gaaatacaaa aaacgactga atcaaatccg ataaaaatat ttttatcatc aagcattaat
    46321 acttttgggg gaatacttat gcaagaaaca gctaatccaa tacctatatt gaattcttta
    46381 taaaaagaaa gaataaaaca tcgataaatc ccttttcctc cctcttgtag aataatatat
    46441 ttttgatagg tgtttattaa atagtcttct aactctctta tggagttata aggatatttc
    46501 gttaatctca ttattttgct ccatgtctaa aacgtcctgt tgatccatat atttcacccg
    46561 ttatgagtct gttcccatct catatagttt caagtttaac aaccccttga ggctatgtta
    46621 aaagagtttt tctaataact attccgtttt cttaaatata aaaaaataga aaaatagccc
    46681 gggtatagtt tgaacaaggg aaatcagacc ggttccaact cacttaatcc gggcaagtcc
    46741 gcgaagccgc gttcacgtat gatttggcaa aagttgttca gataactttt atctgtgtct
    46801 tcagtgcgga tggcggcata cagtttgctt tgcagtccgt cggcagtaat ttggcggtgg
    46861 acgacatagc ctttttcaag gtagggcatg actgtccaat agggaagggc ggcaatgcca
    46921 cgtctgctgg caaccagttg gataatggcg atggtcagct cgctgtgccg gcgcggcggg
    46981 ttgatgtttt tcggaatcag gattttcttg ggcaaatcca gcatttcatc gggaacggga
    47041 taggtaatca gggtttcccc gataaagtct tccgccgtcc aaacgttttt ggcggcaagc
    47101 ggatggtccg gggcgcaaat gccgaccatt tcgtaggcaa acagtgggtg gaagctgatg
    47161 ctgctttgtt tttcggcttc ggagacaatg gcgaggtcgg cgcggtgttg cagcagcagc
    47221 ccgacgggat ccgcttggaa tcctgatacg atatccagtt cgacttgggg ccacatcggg
    47281 cggaattcgc ccatggcggg catcagccag tcgaaacagg tatggcattc gacggcaatc
    47341 cgcagctctc ccgcctcccc ttccgtgatt cgcgccaaat ctcgttctgc aacagcaact
    47401 tgaggtatca gttcgtgggc gaggcgcagc agcctttcgc ccaccggggt aaagcgcagg
    47461 ggcgtggatt tgcgttcgaa cagcggcgtg ccgtagtggt tttcgagcat acggatctgg
    47521 tgggaaaggg cggattgggt aaggaaaacc cgtttggctg caagggagac gctgccggtt
    47581 tcttcaagtg ccagcagggt tttgaggtgg cgcaattcga taatggaatc catggggctt
    47641 cagacggcat attgaaccgg cgcatattaa aataattcat atgcgggtgc aaatcggaaa
    47701 aatgggcgtt tcgggcattt gcggttagaa tgcccgcttg tttggaaatg acaggaaaaa
    47761 tatggttttg cgcagagatt ttttagcctg gtgcgacgaa acgttgcaga cggcatcgtt
    47821 taaagattac gcccccaatg gtttgcaggt tgaagggagg gaatatatcg ggaaaatcgt
    47881 tacgtcggta acggcaagca gggcagcgat tgattttgct gtggagcaga aggcagattt
    47941 gcttttggta catcacggta tgttctggaa aaacgagttg ccgaccgtta ccggttggaa
    48001 aaaagaacgg attgccgcac tgttacggca cgacatcaat atggcaggct accatctgcc
    48061 cctggatgtg catcccatac tgggcaacaa tgcccaactc gccgacagat tgggttttgc
    48121 gacagaaaaa cggttcggcg aacaaaacct gctcaactcg ggcagcctga aacaagccaa
    48181 gacactcggc gcattggcgg cgcatattga aacagttttg caacgtaagc ctgtcgccat
    48241 cggcaaaccc gaacgcgaaa tccgacgggt cgcatggtgt accggcggtg cacagggctt
    48301 ttttcagacg gcaatagatg aaggcgtcga tctgtatctg acgggtgaaa tttccgaagc
    48361 ccaataccac ctcgccaatg aaacgggtac ggctttcatt tcggcagggc atcacgcgac
    48421 ggaacgttac ggcgtacgcg cgctggcaga atcggcggca gaggttttcg ggttggaagt
    48481 gtgccatttt gacgaaaaca acccggcttg agtcttgaga aatatcataa aactttacct
    48541 tattttaaat aatgctttga gtatcactgc aaactgggta aaattgcaat gtttaatggc
    48601 tcggcattcc caaaatatta ggacgactga ataatggata atcaagaaat caacaacggc
    48661 cgccgccgtt tcctgacact tgcgacctgc ggcgcgggcg gagtggcagc attgggtgtg
    48721 gcaacgccgt ttgtggccag ttttttccct tcggaaaaag ccaaggccgc cggtgctgcc
    48781 gtcgaagtgg atgtcagtaa aatcgaagcg ggtcagctgc tgaccgccga gtggcaaggc
    48841 aaaccgattt gggtgctcaa ccgtacagat cagcagctta aagacctgaa aggcctgaac
    48901 ggcgaactta ccgatcccaa ttccgatgcg gaacagcagc cggagtatgc taaaaacgag
    48961 acccgttcga ttaagccgaa catccttgtc gccatcggta tctgcaccca tttgggctgc
    49021 tcgcccacct tccgtcccga cattgccccc gccgatttgg gcgcagactg gaagggcggc
    49081 ttcttctgcc cgtgccacgg ttcgaaattc gacttggccg gccgcgtata taaaggtgtt
    49141 cctgccccga ccaacctggt tgtcccgcca tataaatact tgagcgacac aactatcttg
    49201 gtgggcgaag actaagaata aggaacgata attatggcaa accaaaccaa tagcaaagca
    49261 aaagcattgt taggctggat ggacgctcgc ttccccctgt ctaaaatgtg gaatgagcat
    49321 ttggcgcaat actatgcgcc gaaaaacttt aatttttggt actatttcgg ctctttagcc
    49381 ctgctcgtcc tcgtgattca aatcgtcagc ggtattttcc tgacgatgaa ctacaaaccg
    49441 gacggcaacc tgaatgccta ccatctgcct gctgccttta ccgcagtaga gtacatcatg
    49501 cgcgacgtgt ccggcggttg gattatccgc tacatgcatt ccaccggcgc atctttcttc
    49561 ttcattgttg tttatctgca catgttccgt ggtttgattt acggttcgta caaaaaaccc
    49621 cgtgagttgg tgtggatttt cggttctctg attttcttgg cattgatggc ggaagccttt
    49681 atgggctacc tgctgccttg gggtcagatg tccttttggg gtgcgcaggt gattatcaac
    49741 ctgttctccg ccattcccgt catcggtcct gatttgtcca cttggattcg cggcgacttc
    49801 aacgtttccg acgttaccct gaaccgcttc ttcgccctgc acgttatcgc cgtaccgctg
    49861 gtattgctcg gtttagtcgt ggcacacatc atcgcactgc acgaagtggg ctctaacaac
    49921 cccgacggtg tggaaatcaa gaaaaacaaa gatgaaaacg gcattccgcg cgacggcatc
    49981 ccgttccacc cttactacac tgttaaagac atcctgggcg tcgttgtatt cctgattgtc
    50041 ttctgtgccg tattgttctt tgcccctgaa ggcggcggct acttcctgga agcgccgaac
    50101 ttcgatgcgg caaatgcgct gaaaacaccc ccgcacattg cgccggtatg gtatttcaca
    50161 ccgttctacg ccattttgcg tgcgattcct tccttcttgg gaacgcaggt ttggggtgta
    50221 atcggtatgg gcgcggcggt cgtgttgatt gccctgctgc cttggctgga caggggcgag
    50281 gtgaaatccg tccgttaccg cggtccgatt ttcaaaaccg cattggtctt gttcatcatt
    50341 tcctttatcg gtttgggtat tttgggcgcg atggtggcaa ccgatacgcg tactttggtc
    50401 gcgcgcatcc tgtccttcgt ctatttcgca ttcttcctgg gtatgccgtt ctataccaag
    50461 ctggataaaa acaaaccggt tcccgaacgc gtaaccatga gcaccgccaa acaaaaaatc
    50521 atgttctttg tttacgtcgg cattactgcc gtgggtgctt acctgtttgc aaccaacatc
    50581 tgatgagggc agtcgaaatg aaacaagcta tgaaaaattg gtttgctgcc ttgctgtcgg
    50641 cagtacccat gagtgcggca tttgcttcgg gcggtcatgc acactatgag aaagtcgata
    50701 ttgatttgcg cgaccaagta agcttgcagc gcggcgcaca aatctttacc aactactgtt
    50761 tgtcctgcca ctcggcaagc ggtatgcgtt tcaaccgttt gaaagacatc ggtctgactg
    50821 atgaagaaat caagaaaaac ctgatgttta ccaccgataa tgtcggcgat gtcatgcatt
    50881 cggcgatgaa cccgaaagat gcggcaaaat ggtttggtgc tgctccgccc gatttgacat
    50941 tgattgcgcg ttccaaaggt gcagactacc tttacgctta tatgcgcggc ttctataaag
    51001 atccgacccg tccgagcggc tggaacaata ctgtattcga taaagtcggt atgccccacc
    51061 cgttgtggga acagcaaggc gttcaagccg ttgagttgga tgccaaaggt cagccggtta
    51121 tggtaaaaga cgaacacggc gagatgaagc ctaagctgta ttgggaatct accggtttgc
    51181 acagccgccg tctgcctaac ggcaaagtga tccagaaaga gtacgacgca tatgtacgcg
    51241 atttggtcaa ttaccttgtg tacatgggcg aacctgcaca actgcaacgc aaacgtatag
    51301 gctatgtcgt gatgattttc ctatttgcgg ttatgctgcc tttggcgtat ttcctgaaaa
    51361 aagaatattg gaaagacgta cactaagcgt ttggaacaaa agggcaaatc ctttagggtt
    51421 tgcccttttt tcattttgcc tgccgtttga aaagcctgaa tccgtatgcc gtctgaacat
    51481 agctgcaaca tttcagacgg catcattcta aaaatgtcag gcatcgggaa cttaatcagg
    51541 gtttgtggca gattgatatt gaattaggaa aaggtctgaa accgcaagca gcatggtaag
    51601 gcgatgtcga acaggtttaa agccaatccg ctatattttt tggagctgca ccgtgcaaac
    51661 aggtagaatg gcggcggttt tgtccggtgt gaaaatgttt gaatattagg atgattgatg
    51721 gagctgatga ctgttttgct gcctttggcg gcgttggtgt cgggcgtgtt gtttacatgg
    51781 ttgctgatga agggcaggtt tcagggcgag tttgccggtt tgaacgcgca attggcggaa
    51841 aaggcggcaa gatgtgattt tattgaacag gcacacgcag aaattgcatc agaattggcc
    51901 gctttggatg ggaaatacga gcatttgcag gacgaaaatt atgctttggg caaccgtttt
    51961 tccgcagccg aaaagcagat tgcccatttg caggaaaaag aggcggaatc ggttcggttg
    52021 aagcagtcgt atatcgattt gcaggaaaag gcgcagggtt tggcggttga aaacgaacgt
    52081 ttggcaacgc agctcgggca ggaacggaag gcgtttgccg agcaatatgc gctggaacgc
    52141 caaatccgcc aaagagtcga aaccgatttg gaagaaagcc gccaaactgt ccgcgacgtg
    52201 caaaacgacc tttccgatgt gggcaaccgt tttgccgccg ccgaaaaaca gattgcctat
    52261 ttgcaggaaa aagaggcgga agcggagcgg ttgaggcagt cgcataccga gttgcaggaa
    52321 aaggcgcagg gtttggcggt tgaaaacgaa cgtttggcaa cgcaaatcga acaggaacgc
    52381 cttgcttctg aagaaaagct gtccttgctg ggcgaggcgc gcaaaagctt gagcgatcag
    52441 tttcaaaatc ttgccaacac gattttggaa gaaaaaagcc gccgttttac cgagcagaac
    52501 cgcgagcagc tccatcaggt tttgaacccg ttgaacgaac gcatccacgg tttcggcgag
    52561 ttggtcaggc aaacttatga taaagaatcg cgcgagcggc tgactttgga aaacgaattg
    52621 aaacggctcc aagggttgaa tgcgcagctg cacagcgagg caaaggccct gaccaacgcg
    52681 ctgaccggca cgcagaacaa ggttcagggc aattggggcg agatgattct ggaaacggtt
    52741 ttggaaaatt ccggccttca gaaagggcgg gaatatgtgg ttcaggcggc atccgtccga
    52801 aaagaggaag acggcggcac gcgccgcctc cagcccgacg ttttggtcaa cctgcccgac
    52861 aacaagcaga ttgtcattga ttccaaggtt tcgctgacgg cttatgtgcg ctacacgcag
    52921 gcggcggatg cggatgaggc ggcacgcgaa ctggcggcgt atatcgccag catacgcgcg
    52981 catatgaaag gcttgtcgct gaaggattac accgatttgg aaggtgtgaa cacattggat
    53041 ttcgtcttta tgtttatccc tgtcgaaccg gcctacctgc tggcgttgca gaatgacgcg
    53101 ggcttgttcc aagagtgttt cgacaaacgg attatgctgg tcggccccag tacgctgctg
    53161 gcgactttgc ggacggtggc gaatatttgg cgcaacgagc agcaaaacca aaacgcgctg
    53221 gcgattgcgg acgaaggcgg caagctgtac gacaagtttg tcggcttcgt acagacgctc
    53281 gaaagcgtcg gcaaaggcat cgatcaggcg caaaacagct ttcagacggc attcaagcaa
    53341 cttgccgagg gacgcgggaa tctggtcgga cgcgccgaga aactgcgtct gttgggcgtg
    53401 aaggcaggca aacagcttca acgggatttg gtcgagcgtg ccaacgaaca gacggcattt
    53461 gctttgggca aaccttcgga acaggaagcg gctgacgaag ccgaatagga aaggcggatg
    53521 cgtttcagac ggcatcagtg cagatgatgc cgtctgaaga ctttgggaag tcggttagaa
    53581 aaacggattg tgccgttttt cgtgtccgat ggaagtcata cgcccgtgtc cggcgacaac
    53641 ttgcacggtt tcggggaagg cgaataattt gttgcggata ttgttgatta agtcggcgtg
    53701 gttgccgcgc ggaaaatcgg tcctgcctat ggtttcgtaa aacagcacgt cgcccgcaat
    53761 cagcagctcc gcttcggcac aatagaaaac cacgtgtccg ggcgtgtgcc ccggaatatg
    53821 cagcacttga aaggcatagc gtccgaccgt gagcgtttcg ccttcttcga gccaacggtt
    53881 cggcgcaaag gcgggcgaga cgggaaatcc gtattgcgcg gtggtttgcg gcagcgattg
    53941 gagcaggaat tcatcttcgc ggtgcggtcc gaggacggga acgttacgcg ttttcaacat
    54001 ttcgaccacg ccgcccgcgt gatcgagatg gccgtgcgtc agccagattg ccgtgagcgt
    54061 aagtttgcgg tttgccaacg cttgcagcag gaacggcacg tcgccgccga catcggtcag
    54121 gacggcttcg ccgctttcgt cgtcccaaat cagggtgcag ttttggcgga agggggtaac
    54181 ggggaagatt tcgtaacgta gggtcatcgg cgtgttccta aacggttttt cagacggcat
    54241 cgggtttgcc gtttgtttta tggcggtttg ccgcctgttt tgatattggg ggaatcaggt
    54301 gattaagaag ataatcggcg gcatcatacc gatttttacg gcggttttca tccctgcatc
    54361 ggcaggcgcg gcggatttga tgctggcgca ggaatacaaa gggcaggaca ttgccggctg
    54421 ggcgatgagc gagaagctcg acggcgtgcg cgcctattgg gacggaaagc acctgataag
    54481 ccgtcagggc tacgcgttta cgccccccaa aggctttacc gcgcagtttc cgccttatcc
    54541 tttggacggc gaattgtata gcgggcgcgg tcagttcgag cagatttccg ctaccgtgcg
    54601 ttctgtttct tcagactggc gcggcatccg cctgcacgtt ttcgatgtac ccaaggcgca
    54661 gggcaacctc taccaacgtt tggcagtcgc aacgcagtgg ctgaaaacgc atccgaacgc
    54721 gccgattacc atcatcccgc aaatcaaagt gcgcgaccgg cggcacgcga tggacttttt
    54781 aaaacaaatc gaagcgcagg gcggcgaagg cgtgatgctg cgccagcccg aatcccgtta
    54841 cagcggcggc aggagcagcc aattattgaa gctgaaaagc caatacgacg acgaatgcac
    54901 ggtaacgcgg cactatgagg gcaaagggcg aaacgccgga cggttgggcg cggtcggctg
    54961 caaaaaccga cacggcgaat tccgcatcgg catcggtttc aaagacaaag accgcgacaa
    55021 tccgcccaaa atcggcacgc tgattaccta ccgctaccgt ggctttacgc ggaaaggcac
    55081 gccgaaattt gccacatttg tgcgcgtgcg taccgaccgc tgagcaggac agttcagacg
    55141 gcatccgata ctttggttta taattttcct tttaccgacc gattccgaca tatgaccgat
    55201 ttagaaacca aacgccttga aacacaggcg atgcttgaaa acgccgatct tttgttcgac
    55261 caaggccaat gccgtgccgc actgcaaaaa gtggcggacg agattacgcg cgatttgggg
    55321 gataaatatc cgttgctcct gcccgtgatg ggcggggcgg tggtgtttac ggggcagttg
    55381 ctgccgctgt tgcgctttcc cttagacttt gattatgttc acgtttcccg ttacggcgac
    55441 aagctggagg gcggcgcgtt caactggaag cgtatgcctg atgcggaaca aatccggggc
    55501 aggcacgtgg tcgtgttgga cgatattttg gacgaagggc atacgatgtc cgccattcaa
    55561 gccaaacttt tggaaatggg tgcggcaagc tgccgtgcgg cggtgttcgc caataaatta
    55621 atcgacaaag aaaaaccggt caaagccgat tatgtcggac tggatgtgcc gaaccgttat
    55681 gttttcggtt acggcatgga tgcggcgggc tgctggcgta atttgggcga gatttacgca
    55741 ttgggcggaa aataagggat gcgatgccgt ctgaaggcct ttcagacggc atcgcgccca
    55801 tacgccggca ggattaatga ggaacaggac gcatcaatat gatagggctt ttaatcatca
    55861 cacacgaaac cataggcgaa gcctaccgca agctggcgca tcattttttt ccgggcgggc
    55921 tgcctgaaaa cgtccgcata ctcggcgtgc agccgacgga aggccaagac gacattatca
    55981 acaacgccat ttccgcgctg caagagtttc ctgaaaacca cggtgtattg attatgaccg
    56041 acatcttcgg cgcgaccccc tgcaatgccg cccgccggct cgtgcgcgaa aacaaatcgg
    56101 cgattttgac cgggctgaac gcgccgatga tgattaaggc cgtccaatat tcgccggcgg
    56161 cggaagactt tgccgccttt accgaatgcg tcagagaggc ggcggtaaaa ggcattttcg
    56221 ccatcacgtc cgcgcccgaa gatttggtgt gccggcgcag cggcgatgcc gtctgaagaa
    56281 gcggcagggc aggaaaacat tttaaacggc gtctgctgcc gatatatagc ggattaacaa
    56341 aaatcaggac aaggcggcgg gccgcagaca gtacggatag tacggaaccg gttcgcccgg
    56401 tgcttcagca ccttggagaa tcgttctctt tgagccaagg cggggcaacg ccgtactggt
    56461 ttttgttaat ccactataca taacacggga accgaaatgc tcaaacaatc catcgaaatc
    56521 atcaacaaac tcggactcca cgcccgcgcg tccagcaagt tcacccaaac cgcgtcccaa
    56581 ttcaaaagcg aagtctgggt tacgaaaaac ggcagccgcg tcaacggcaa aagcattatg
    56641 gggctgatga tgctcgcagc cgccaagggt acggtcatcg aactggaaac cgacggcttg
    56701 gacgaagccg cagccatgaa ggcattgacc gacttaatca acgactactt cggcgagggc
    56761 gaataatgag tatcgtgctg cacggcgtgg cggcgggcaa aggcattgcc gtcggttgcg
    56821 cccacctgat tgcgcgcggt acggaggaag tgccgcagta tgatgttgct caggcggaca
    56881 ccgatgccga agccgaacgt ttcgatgccg ccgtcaaagc cacgcgcaaa gagttggaac
    56941 agctccgcag cgcgattccc gaaaacgccc cgaccgagtt gggcgcgttc atctcgctgc
    57001 acctgatgct cttgaccgat gttaccttgt cgcgcgaacc cgtcgatatt ttaagggaac
    57061 aaaaaatcaa cgccgagtgg gcattgaagc agcagagcga caaactcgcc gcccaattcg
    57121 acaatatgga cgatgcctat ttgcgcgaac gcaagcagga tatgctgcaa gtcgtccgcc
    57181 gcatccacaa caacctgatc gggcagggca acgagttgga ggttgccgac aacctgtttg
    57241 acgaaaccgt tctgattgca aacgaccttt cacccgccga cacggttttg tttaaagagc
    57301 agcgcattgc cgccttcgtt accgatgccg gcggccccac cgggcatacg gcgattttgg
    57361 gcaggagctt ggacatcccg tccgtcgtcg ggctgcacaa cgcgcgcaaa ctgattaccg
    57421 aaggcgaaac ggtcatcgtg gacggcatca acggcgtgtt gattatttcg ccggatgagt
    57481 cggtgttgaa cgaataccgc cgccgcgccc gcgaataccg cagccacaaa cgcgatttga
    57541 acaagctcaa aaaaaccgcc gccgccaccg ccgacggggt ctgcatcgag cttgtgggca
    57601 atatagaatc cgccgaagac gtgaaaccgc tgcacaacct cggcgcagac ggcatcgggc
    57661 tgttccgcag cgagtttctt tacctgaacc gcgatacgat gccgtctgaa gacgagcagt
    57721 acgaagtgta cagcgcgatt gtcaaaaaaa tgaagggcaa aagcgtaacg atacggacag
    57781 tcgatttggg tgtggacaaa aatccgcgct ggttcgggaa aaacagcacg cccaacggca
    57841 gcctcaaccc cgcgctgggc atgaccggca tccgcctgtg ccttgccgaa ccggtcatgt
    57901 tccgcacgca gatgcgcgcc atcctccgtg cggcggtaca cggtcccgtc cgcatgatgt
    57961 ggccgatgat tacctccgta tccgaagtgc gccagtgcct catccacctc gacaccgcgc
    58021 aacgccagct tgccgaacgc ggcgatgcct tcggcgaagt cggcatcggc tgcatgattg
    58081 aaattccgtc tgccgcgctg accgtcggca gtattttgaa actggtcgat ttcatctccg
    58141 tcggtaccaa cgatttgatc caatatattc tgtccgtcga tcgcggcgac gacagcgtca
    58201 gccatctcta ccaacccgga catccttccg tgctgaaaat gctgcaacac gtcatccgta
    58261 ccgccaaccg catggacaaa gacgtgtccg tatgcggcga gatggcgggc gataccgcgt
    58321 ttacccgcgt tttattgggt atggggctgc gccgtttttc catgaatccg aacaacatcc
    58381 tgcccgtcaa aaacatcatt ctgcacagca atgccggaca gctcgaaggc gatattgcga
    58441 aagtcatccg ctgcgaagac gaagagaagt cggaaaagct gatcaaacag atcaacagcg
    58501 tgtctgtcgc ggaagaagcc gaagtcaagg gacggaaata aatattgttg acaatgcgag
    58561 cgggttgcag tcaaataacg cccttaaaaa atacagctgc tttggtcaag aaatgccgtc
    58621 tgaaaaattt tcagacggca ttttgtttgg caggcatttc tgccgcatca tcggaaacgg
    58681 acgatacgcc cttcatcgac gcggatgcgg accgtaccgt ttccggaaat accgtccgta
    58741 tgttgtccga cagtcaggtt taaggtcagc tcgccgtgtt cgggatggac ggcggaaagc
    58801 cggagcgagt cgggcaggcg gacgagggac agcaggcggc attccgttcc atgattgtcc
    58861 aagcacacgg cattttgcgg aatatggcgg tcatcgtcgg tattgggcag ccccatcaga
    58921 cgggcgacct gcacgccggc aggcgtttga atcaaggttt cgggcgtacc gcattgaagg
    58981 attttcccct cgtgcatgac ggcgatttcg tccgccgccg tgcaggcctc ttcgggcgaa
    59041 tgcgttacca aaacggcagg gatgccgccc ttgcggatgc gttcggcggt catacggcgc
    59101 agccggtcgc gcaaatgcgt gtccaaactg gaaaacgatt catccagcaa cagcagggaa
    59161 gggcggacaa ccaaagcgcg cgccagtgcc aaccgttgct tctcgcctcc ggaaagtttt
    59221 tcaggcttgc ggtgcgcctc gttttccagc ccgacttcgg caagtgccga caaggcgagg
    59281 cgttcggctt cggctttcgg cattttttgc attttcaaac cgaatgccgt attttccagc
    59341 gcactcatat ggggaaacag cgcgtaatct tgaaacatca gcgagatacg gcgtttttcg
    59401 ggcggcatac aggtaatgtt ttccccgttc agccgaattt cgccgccgtc cggccggacg
    59461 atgcccgcaa tcatattcag cagggtggat ttgccgcagc ccgaccgccc cagtacggcg
    59521 agtattttgc cgcgcccgac agtcaggcag atgttgtcgg cgaccgtttt gccgccgaag
    59581 catttgcaga gtccgttcag ttcaagcatg gcgcatccta taaacgtatg ccgtccagcc
    59641 actcggacag cggatgggcg gcatcaatca gaccgtaacg gtaaaacgcg tccagcgtaa
    59701 ccagttgcgc gtcgtgcatc atgtttttcg acaacatggc atccaatagg ccgccaatgt
    59761 ccatcttttc aaaacccgct acctcgccat cctgattttc aggcaggaag gtttcgggca
    59821 ggacggcatc gaacacatac aggatttcat tgtgcacacc tcggctgacg gggcgaaggc
    59881 tgtgcagccg cgatactggg cggatgagcg gaaacagcgt tttatccaaa ccggcttctt
    59941 cgctgctttc gcggcacacg gcttcagacg gcatttcgcc gccggaaaca ccgccgccgg
    60001 caatattgtc gagcttgccg ggatcgactg ctttgtgcgg actgcgcctg cctatccaaa
    60061 aatgccatct gccgttcgat tcgaccaaac cgttgagatg gacggcgcgg ctgagtagtc
    60121 cgaacggacg gaaagcggcg cgttcgagcg tgaacaaggg gttgccgccg ccgtcggtca
    60181 ggtcgaaaca ctcgttgcgc catccgtgaa gcagccccgc cttgttccat gtgcgggcga
    60241 ggtgctgcaa gcgtccgccc atatccggcc agccgtccgc attcagaaaa atgccgtctg
    60301 aagactcgga gcagcctgcc tcccagtctt ttttgatgcg ctcagcccat tccggcgaca
    60361 gattgcccaa aggaagacgg ttcagataca gcgttttcca gcaactttcg gcaccgtaac
    60421 ttgcttttgc ccgctcgaac agggcatcaa ggtcttgttt gctgacggat tcggtaaaac
    60481 ggacggtcgg catagccggc tttcttgaac acaatgcgga agattgtagc caaaccgcct
    60541 gaatttgcaa ccgcaaacag acggcggcct gccgttccga tattgcggca ggaaggaaaa
    60601 tccggcaaaa aacggaggcg gcaggaaaca ggaaaaacaa aaccccctcg gcattgaacc
    60661 gaggggtttg atatttggtt gcgggagcag gattcgaacc tacgaccttc gggttatgag
    60721 cccgacgagc taccatgctg ctccatcccg cgtcagaaga tgaaactata cggcagattt
    60781 tttatgttgt caaacattat ttccgtgaaa aatcataaag ttttccgtcc ggcggcttat
    60841 gcctgatttg aaatatcatt ttctttacaa aagttcatgt tcgtgattta attttggtta
    60901 acatcgaaac aggggtgctg cctgatgttc aggcggctga gaaataccct ttacacccga
    60961 tcgggataat acctgcgtgg ggagttttca cggattctgt ctttcagacg gcattggttt
    61021 tcaaatgccg tctgaaaacg caaaacgctc ctgtttcttt attctaaacg agaaaacagg
    61081 agcatttttt atgactacgc caaaaaaaac cgccaaaact tccggcaacg aagcgcgcga
    61141 actttccgac ttgagcgaag acatcggcat ccgcttcaaa tatcagaact ccgaacgcgt
    61201 gtatctgcaa ggcagccgcg acgacatccg cgtgcctttg cgcgaaatcc gtcaggacga
    61261 cacctacacg gcgcaaggta cggaagccaa cccgcctatt ccggtgtacg acaccagcgg
    61321 cgcatacggc gatccggcgg cgcacatcga cctgaaacaa ggcctgccgc acatccgcac
    61381 ggcgtggctg gacgaacgcg gcgataccga aatcctgccc aagctctcca gcgaatacgg
    61441 catcgaacgt gcgcacgatc cgaaaaccgc ccatctgcgc ttcaaccaaa tcacccgccc
    61501 gcgccgtgcc aaagccggcc gcaatgtaac ccagctccat tacgcccgcc aaggcattat
    61561 cacgcccgaa atggagttcg ccgccatacg cgaacgcatg aagctggacg agcttttcag
    61621 acggcctgaa tacgccaagc tcttgaaaca gcacacaggg caaagtttcg gtgcgaacat
    61681 cccgacccgt cccgaccaaa tcacgcccga attcgtgcgc caagaaatcg ccgccggccg
    61741 cgcgatcatc cccgccaaca tcaaccaccc cgaactcgaa ccgatgatta tcggccgcaa
    61801 cttccgcgtc aaaatcaacg gcaacttggg caattccgcc gtaacctcca gcctgaccga
    61861 agaagtcgaa aaaatggtgt ggtcgctgcg ttggggcgcg gacacgatta tggacttatc
    61921 cacaggcgcg cacatccacg aaacgcgcga atggattatc cgcaacgcgc ccgtccccat
    61981 cggcacagtg ccgatttacc aaactttgga aaaaaccggc ggcatcgccg aagatttgac
    62041 ttgggatttg ttccgcgaca ccttaatcga gcaggcggaa caaggcgtgg actatttcac
    62101 catacacgcg ggcgtgttgc tgcgttatgt gccgatgacc gccaaccgcc tcaccggcat
    62161 cgtatcgcgc ggcggttcga ttatggcgaa atggtgtttg gcacatcatc gggaaaactt
    62221 cctctacacg catttcgacg aaatctgcga aatcatgaag gcgtatgacg tatcgttcag
    62281 cttgggcgac ggcctgcgcc ccggctgcat tgccgatgcc aacgacgaat cccaattcgc
    62341 cgaattgcac accttgggcg aattgaccga taaagcgtgg aaacacgacg tacaagtcat
    62401 gatcgaaggc cccggccatg tgccgctgca acgcgtcaaa gaaaatatga ccgaagagtt
    62461 gcaacactgc tttgaagcac cgttttacac actcggcccg ctcgttaccg acatcgcccc
    62521 cggctacgac cacatcactt cgggcatagg cgcggccaat atcggctggt acggcacggc
    62581 gatgctttgt tacgttaccc ccaaagaaca tttggggctg cccgacaaag aagacgtgcg
    62641 caccggcatc atcacctaca aactcgccgc ccacgccgcc gacctcgcca aaggctggcc
    62701 gggcgcgcaa ttacgcgaca acgccctgag caaggcgcgt ttcgaattcc gctggcgcga
    62761 ccaattccgc ttaagcctcg accccgaacg cgccgagagc ttccacgacg aaaccctgcc
    62821 cgccgaaggc gcgaaaatcg cccatttttg ctcaatgtgc ggccccaaat tttgctcgat
    62881 gaaaatcacg caggaagtgc gcgactacgc cgacaagcaa aaagcccaac ggcagggtat
    62941 ggaggaaaaa gcggtcgagt tcgtcaaaaa aggcgcgaag atttacagtt gaaacgtcaa
    63001 gcaaaaaatc ccgtctgaag cccggaaaaa aggcttcaga cgggattttt tcgcttgtga
    63061 aaatgattaa acaaccgatc attaaagtaa tttttaaaat ttttatattc acatagatgg
    63121 gaatgatggg atttagggtt ctgattttgt tttgagagaa tgaaggaatt tgagattgtg
    63181 ggcgattatc gggaaaaata gaatctttc
//
LOCUS       DAXDZG010000003        56790 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXDZG010000003 DAXDZG010000000
VERSION     DAXDZG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.003"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="ACFB78_000132"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="ACFB78_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC1268230.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="ACFB78_000133"
     CDS             complement(1966..2568)
                     /locus_tag="ACFB78_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC1268231.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPSTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="ACFB78_000134"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="ACFB78_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC1268232.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="ACFB78_000135"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="ACFB78_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC1268233.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(4526..5488)
                     /gene="hprK"
                     /locus_tag="ACFB78_000136"
     CDS             complement(4526..5488)
                     /gene="hprK"
                     /locus_tag="ACFB78_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC1268234.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5492..5941)
                     /gene="ptsN"
                     /locus_tag="ACFB78_000137"
     CDS             complement(5492..5941)
                     /gene="ptsN"
                     /locus_tag="ACFB78_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC1268235.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(6126..7016)
                     /gene="ubiA"
                     /locus_tag="ACFB78_000138"
     CDS             complement(6126..7016)
                     /gene="ubiA"
                     /locus_tag="ACFB78_000138"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC1268236.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7006..7413)
                     /locus_tag="ACFB78_000139"
     CDS             complement(7006..7413)
                     /locus_tag="ACFB78_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268237.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(7422..7517)
                     /locus_tag="ACFB78_000140"
     CDS             complement(7422..7517)
                     /locus_tag="ACFB78_000140"
                     /inference="COORDINATES: protein motif:HMM:NF026993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HFC1268238.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(7537..8013)
                     /locus_tag="ACFB78_000141"
     CDS             complement(7537..8013)
                     /locus_tag="ACFB78_000141"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC1268239.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(8028..8675)
                     /gene="bioD"
                     /locus_tag="ACFB78_000142"
     CDS             complement(8028..8675)
                     /gene="bioD"
                     /locus_tag="ACFB78_000142"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC1268240.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(8672..9973)
                     /gene="bioA"
                     /locus_tag="ACFB78_000143"
     CDS             complement(8672..9973)
                     /gene="bioA"
                     /locus_tag="ACFB78_000143"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC1268241.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(10191..10415)
                     /locus_tag="ACFB78_000144"
     CDS             complement(10191..10415)
                     /locus_tag="ACFB78_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268242.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            complement(10607..11092)
                     /locus_tag="ACFB78_000145"
     CDS             complement(10607..11092)
                     /locus_tag="ACFB78_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC1268243.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(11268..11576)
                     /locus_tag="ACFB78_000146"
     CDS             complement(11268..11576)
                     /locus_tag="ACFB78_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268244.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(11560..11862)
                     /locus_tag="ACFB78_000147"
     CDS             complement(11560..11862)
                     /locus_tag="ACFB78_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC1268245.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(11936..14299)
                     /gene="pheT"
                     /locus_tag="ACFB78_000148"
     CDS             complement(11936..14299)
                     /gene="pheT"
                     /locus_tag="ACFB78_000148"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC1268246.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(14390..15139)
                     /locus_tag="ACFB78_000149"
     CDS             complement(14390..15139)
                     /locus_tag="ACFB78_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1268247.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIENLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWREDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(15145..15426)
                     /locus_tag="ACFB78_000150"
     CDS             complement(15145..15426)
                     /locus_tag="ACFB78_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268248.1"
                     /translation="MGLPYQNKDFRNMRLSDYILNNKININNLSDKQVQEIIDYFSSE
                     IDDWLLASEKELKDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(15419..15637)
                     /locus_tag="ACFB78_000151"
                     /pseudo
     CDS             complement(15419..15637)
                     /locus_tag="ACFB78_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(15744..16157)
                     /locus_tag="ACFB78_000152"
     CDS             complement(15744..16157)
                     /locus_tag="ACFB78_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC1268249.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(16174..17163)
                     /gene="pheS"
                     /locus_tag="ACFB78_000153"
     CDS             complement(16174..17163)
                     /gene="pheS"
                     /locus_tag="ACFB78_000153"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC1268250.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(17404..17763)
                     /gene="rplT"
                     /locus_tag="ACFB78_000154"
     CDS             complement(17404..17763)
                     /gene="rplT"
                     /locus_tag="ACFB78_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC1268251.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(17776..17973)
                     /gene="rpmI"
                     /locus_tag="ACFB78_000155"
     CDS             complement(17776..17973)
                     /gene="rpmI"
                     /locus_tag="ACFB78_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC1268252.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(18120..18641)
                     /gene="infC"
                     /locus_tag="ACFB78_000156"
     CDS             complement(18120..18641)
                     /gene="infC"
                     /locus_tag="ACFB78_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC1268253.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(18659..20572)
                     /gene="thrS"
                     /locus_tag="ACFB78_000157"
     CDS             complement(18659..20572)
                     /gene="thrS"
                     /locus_tag="ACFB78_000157"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC1268254.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(20625..20701)
                     /locus_tag="ACFB78_000158"
     tRNA            complement(20625..20701)
                     /locus_tag="ACFB78_000158"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20665..20667),aa:Val,seq:gac)
     gene            20880..21995
                     /gene="tgt"
                     /locus_tag="ACFB78_000159"
     CDS             20880..21995
                     /gene="tgt"
                     /locus_tag="ACFB78_000159"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC1268255.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGVD"
     gene            22005..23087
                     /gene="hemH"
                     /locus_tag="ACFB78_000160"
     CDS             22005..23087
                     /gene="hemH"
                     /locus_tag="ACFB78_000160"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC1268256.1"
                     /translation="MPSEAASDGILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            23278..23745
                     /locus_tag="ACFB78_000161"
     CDS             23278..23745
                     /locus_tag="ACFB78_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFC1268257.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTAFPPLFRSDYIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQTKPF"
     gene            complement(23742..25463)
                     /locus_tag="ACFB78_000162"
     CDS             complement(23742..25463)
                     /locus_tag="ACFB78_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC1268258.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILADGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGNIGS
                     MGLKVPR"
     gene            25667..26407
                     /locus_tag="ACFB78_000163"
                     /pseudo
     CDS             25667..26407
                     /locus_tag="ACFB78_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            26476..28014
                     /gene="lnt"
                     /locus_tag="ACFB78_000164"
     CDS             26476..28014
                     /gene="lnt"
                     /locus_tag="ACFB78_000164"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC1268259.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28278..29141
                     /gene="rpoH"
                     /locus_tag="ACFB78_000165"
     CDS             28278..29141
                     /gene="rpoH"
                     /locus_tag="ACFB78_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC1268260.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29298..29774)
                     /locus_tag="ACFB78_000166"
     CDS             complement(29298..29774)
                     /locus_tag="ACFB78_000166"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC1268261.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            29874..30758
                     /locus_tag="ACFB78_000167"
     CDS             29874..30758
                     /locus_tag="ACFB78_000167"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC1268262.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(30849..31364)
                     /locus_tag="ACFB78_000168"
     CDS             complement(30849..31364)
                     /locus_tag="ACFB78_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC1268263.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(31440..31859)
                     /locus_tag="ACFB78_000169"
     CDS             complement(31440..31859)
                     /locus_tag="ACFB78_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268264.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(31980..32978)
                     /gene="holA"
                     /locus_tag="ACFB78_000170"
     CDS             complement(31980..32978)
                     /gene="holA"
                     /locus_tag="ACFB78_000170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC1268265.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGNAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(32980..33459)
                     /gene="lptE"
                     /locus_tag="ACFB78_000171"
     CDS             complement(32980..33459)
                     /gene="lptE"
                     /locus_tag="ACFB78_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC1268266.1"
                     /translation="MNKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(33511..34290)
                     /gene="pgeF"
                     /locus_tag="ACFB78_000172"
     CDS             complement(33511..34290)
                     /gene="pgeF"
                     /locus_tag="ACFB78_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC1268267.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            34442..34666
                     /locus_tag="ACFB78_000173"
     CDS             34442..34666
                     /locus_tag="ACFB78_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268268.1"
                     /translation="MMPLYTFSELYMLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGF
                     NRKGMGIKSISDTSRAIPSENQSPLSDGIV"
     gene            34743..35690
                     /locus_tag="ACFB78_000174"
                     /pseudo
     CDS             34743..35690
                     /locus_tag="ACFB78_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(35843..36967)
                     /gene="rluD"
                     /locus_tag="ACFB78_000175"
     CDS             complement(35843..36967)
                     /gene="rluD"
                     /locus_tag="ACFB78_000175"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC1268269.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            36966..37769
                     /locus_tag="ACFB78_000176"
     CDS             36966..37769
                     /locus_tag="ACFB78_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC1268270.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(37839..40058)
                     /locus_tag="ACFB78_000177"
     CDS             complement(37839..40058)
                     /locus_tag="ACFB78_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC1268271.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVATLIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            40090..40365
                     /locus_tag="ACFB78_000178"
     CDS             40090..40365
                     /locus_tag="ACFB78_000178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268272.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            40444..45042
                     /locus_tag="ACFB78_000179"
     CDS             40444..45042
                     /locus_tag="ACFB78_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFC1268273.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLNTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLNQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTGHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGNVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEALR
                     QQAKAEQVKRQQAAEAEKVARQKDEEAKRKAAEIARQQEEARKAAELAAKQKAEAERK
                     ARELARQKAEEASHQANAKPKRRRRRAILPRPPAPVFSLDDYDAKDNSESSIGNLARV
                     IPRMGRELINDYEEIPLEELEDEAEEERRQATQFQPKSRNRRAISSEPSSDEDASESV
                     STSDKHPQDNTELHEKVETAGLQPRAAQPRTQAAAQADAVSTNTNSALSDAMASTQSI
                     LLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRS
                     LSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSR
                     LQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVKVSS
                     MAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQQYS
                     AGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(45118..46320)
                     /gene="trpB"
                     /locus_tag="ACFB78_000180"
     CDS             complement(45118..46320)
                     /gene="trpB"
                     /locus_tag="ACFB78_000180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC1268274.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEATGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(46403..47032)
                     /locus_tag="ACFB78_000181"
     CDS             complement(46403..47032)
                     /locus_tag="ACFB78_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC1268275.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            47308..48087
                     /gene="rsmA"
                     /locus_tag="ACFB78_000182"
     CDS             47308..48087
                     /gene="rsmA"
                     /locus_tag="ACFB78_000182"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC1268276.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            48102..48671
                     /locus_tag="ACFB78_000183"
     CDS             48102..48671
                     /locus_tag="ACFB78_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC1268277.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            48782..49237
                     /locus_tag="ACFB78_000184"
     CDS             48782..49237
                     /locus_tag="ACFB78_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268278.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            49279..50007
                     /locus_tag="ACFB78_000185"
     CDS             49279..50007
                     /locus_tag="ACFB78_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1268279.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            50080..50439
                     /locus_tag="ACFB78_000186"
     CDS             50080..50439
                     /locus_tag="ACFB78_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268280.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            50444..50884
                     /locus_tag="ACFB78_000187"
     CDS             50444..50884
                     /locus_tag="ACFB78_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC1268281.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            50915..52189
                     /gene="folC"
                     /locus_tag="ACFB78_000188"
     CDS             50915..52189
                     /gene="folC"
                     /locus_tag="ACFB78_000188"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC1268282.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            52203..53243
                     /gene="ftsN"
                     /locus_tag="ACFB78_000189"
     CDS             52203..53243
                     /gene="ftsN"
                     /locus_tag="ACFB78_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC1268283.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRSAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            53236..53733
                     /locus_tag="ACFB78_000190"
     CDS             53236..53733
                     /locus_tag="ACFB78_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC1268284.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            53927..55471
                     /gene="purF"
                     /locus_tag="ACFB78_000191"
     CDS             53927..55471
                     /gene="purF"
                     /locus_tag="ACFB78_000191"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC1268285.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYRTGDIDDAYLDRLSAEKSGCAGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            55571..56062
                     /gene="greB"
                     /locus_tag="ACFB78_000192"
     CDS             55571..56062
                     /gene="greB"
                     /locus_tag="ACFB78_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC1268286.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            56121..56747
                     /locus_tag="ACFB78_000193"
     CDS             56121..56747
                     /locus_tag="ACFB78_000193"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC1268287.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT        14403 a        15860 c        13612 g        12915 t
ORIGIN      
        1 cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc tgagtcctgt
       61 ctattatttt agacttctat tttcagttat ttttgtaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaataac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgt atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tgcggaagat gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg cgactttgtt cagcgggcgg ggcggattgc ctttgttagc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag gcaacaggac
      601 gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg tttcgctact
      661 caaacgccct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcagcggcag cttgaagact
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcgggtt caatccggta
      841 tttccgcgcc atccccatca gttcgcccat acgctgttcc tgtgccgcca attcattggg
      901 gttgatgtca ctgtgtcctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ctcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcgg cctgcaacaa ttcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgaga gcttcccatt cgccttgttt aatgtccaac tgattcagtt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc agttttgata aagctgcctg acggtttccg cctgcaccct
     1321 gccgcccaca aatgcgtcca acaattcgcg ctgggcagct tcctgattaa gcgaatgatg
     1381 gtcgttttgc ccgtggatgt cgataagctg cccgccgacg gctttgagtt gcgccaaggt
     1441 agccgcctga ttgttgataa aactgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tccccgccct catccaacag cccttgttca cgcaattctg cttttaaagc
     1561 aggcaaatgg gaaatatcga acaatgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggccttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgccg gtctcgccgg tcaaaacggt aaaaccgctt tgaaaatcca gattcagatt
     1741 ttcgacaatg acaaaatcac gcaaagaaag tgttagaagc attgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttatatctaa gattttttaa
     1861 attaaaaata agtttttcct taattttgct tagtgtttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtagac ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaaaccgt ccaagcgcaa
     2101 atcgctgaga aaagttgccg ttgttgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt ataccttcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcctgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca gttcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttgcgct
     2701 ctgcttttct ggcttcgcga atggctttga gttcggcttc tttctgacgt gcttttttaa
     2761 gccaagtttc ccattggttc ggtgtttcaa gggtgattct gccgattttg ccttcgcgga
     2821 agtccgtgag gatgttttcg gcggcttttt ggtagttgac ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc acgagttgtc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcagca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca atgcgttgcg tccgactgcg ccgccggcgg
     3061 cgaggttgta gccgctttct tcgacgatga ttttcggcca cagcattccc ggggtgtcgt
     3121 acagccagaa gtcgtcggca aggaaaaggc gttgttcggc tttggtaatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca ttccattgat gagggtggat ttgccgacgt
     3241 tgggaatgcc gcaaatcaaa acgcgcaggg gtttgtctat gccttgccgg tggggaatca
     3301 tcgcgcggca ggcttgggtg attttgccgt gtgcgccggt ttcggaggaa tcgagggcga
     3361 tggcccgggt gtcggggcgg ctgttgtagt ggtcaagcca gacctttgtg cgttcagggt
     3421 cggcaaggtc ttgcttgttt aaaattttga gtttgggctt acctttggaa agctgcgcca
     3481 acagtggatt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgcttttggc acgctcggcg atggcttttc ttgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttctcaat atttgaaatg ccgtctgaaa cggcggacgg
     3661 ggtttcagac ggcataatgg tttgacggga ttagcggccc gacaggtttt gcgcctgcct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacga tgctgcccgc cggtacagcc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggcaacc aacgcgtaac aaacctttcg atgccgtcaa ccatttcctg
     3901 cgccaacggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag actgcgcata tcgaacataa aatcggcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaacc accagcagtc cggtacgctc
     4081 aaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacagtag gcgatttcct taagcgggaa cagccattcg cgctcttttt tcaggctttc
     4201 caacagcgtc atatcctgat tgctcaaagg atgtccgcgc cgggtttcgg aaaagcggcg
     4261 gaccagcacg gcctcttccg cctcgacaaa caaaacttcc accctgtgtc cgagtccgcg
     4321 cagataggca atctgctcgc gcgcttgggc aatgtctatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatggtac gacaccagcg acggcagcat
     4441 ttccaagggc aggttgtcca cgcaaaaata gcccaaatct tccatttggc gcagtgcaac
     4501 ggatttgccc gagccggaaa gcccgctaat caggacgatt ttcatgattc tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtctttg ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc aaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc gagctgcttc atatactcgt cgtccgcctc gaccaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccaatgttt
     4921 ccggaccgat gcggaacagc tcgaccgcat cgtcggcaat caggctatgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca aaccagaatg tccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgctgccaag gtgcgttgca
     5101 ggtaaatccg caacacatcc atcagataag ggctttcgag tttggaagtc aacaggggga
     5161 tgtcgttttt gtggcagtag tcgcgcagcc ccggggaaac cggcaaatcg tttgccacaa
     5221 tgaccaaaga catggaaata tcgaacaggt ctccgaactg ataacccgtc tcccccgatt
     5281 cgaggcggtt cagatattcc gactccgcca aaccgaccac ttggatttgg ttgggatgga
     5341 tgaaattcag gtgtccgacc agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccagg caagttgcaa cttgtattgg ttgtcgtcaa
     5461 acaggcggcg gacggagata ctgggcatat tttattcttc ggtcaggatg gcgcgggctt
     5521 cttccgcaga agcaaccgtc atcagcgatt ctctgatgct tttttgggaa aacttgccgg
     5581 ccagtttgga taagacttcc aaatgctcgc ccgttgcgtt ttccggcacc agcaagataa
     5641 aaaccagtga aaccggtttg ccgtcaggcg cgtcgaatcc gacgggttcg cgcgtacgga
     5701 tgaacgcgcc cgtcgcctgc ttcacgctcg cgtggcggcc gtgggggatg gcgacaccct
     5761 gccccaaacc ggtcgaaccg agtttttcac gggcaaaaag acattcgaaa acatcagcat
     5821 gggacaatga ggattcgcgt tccaaaagca ggcccgcttc ctcaaacagc ctttttttac
     5881 tgcccacctc catatccaaa acaatatggg acaaaggcaa aatttcgccg ataaggctca
     5941 taagcttctc ttttcagaca tcgcaaaaca gaaggattgt accgactgcc ggggcaaatc
     6001 tcaatcccgc atacggcacg ggctgacata acacagcgtt tcaaaaacat attttaacgc
     6061 ttttcggcac agatagaaat gccgtccaaa gcagtttacg gttcttcaga cggcattgcc
     6121 ctgccttatt tcgcgaaaaa cgtatgggca aaaatagcgg caaaccacac ccaaccgatt
     6181 ctgttgtttg ccaaaaacgt ttcaaaacag atttgccgga cgcggctttt gatggcggca
     6241 tattggcggt attgcagcag caggacgatg gggattgccg tccaatatgc ccatgccgca
     6301 ccgataaccg cacccaatac tgccatcagc agggtaaagc cgccatgaca cagcataacg
     6361 gcggcgatgt cgtaacgccc gaacgtgacg gcggaggttt tgatgccgat tttcaaatcg
     6421 tcctctttgt ccgccattgc atagacggta tcgtaagcca gtgtccataa aacattggcg
     6481 gcaaagagta tccacgcttc aacgggaacg ctgttgccca ctgcggcaaa cgccatcggg
     6541 atgccgaagg aaaaggcaag tcccagataa aattggggaa tcggaaaaaa gcgtttggta
     6601 aacgggtaag tcagcgcaag gaacagcgcg ggcaggctca tcagccatgt cagatgattc
     6661 agcggaatca ggcacaatgc ggcaagcaga cacaaaaatg ccgtcagcag cagggcttct
     6721 tttttcttga ccctgccctg tgcgaacgga cggtttttgg tgcgctcgac agcaccgtca
     6781 aaatcacggt cggcaaagtc gttgatgacg cagccggcac tgcgcattaa aaacgtgccg
     6841 attgtaaacg ccgccaatac cgccaaatcg ggaatgccgt ctgaagccag ccacaatgcc
     6901 cagtaggtcg gccacagtaa aagcagcgtc ccaatgggct tgtccgccct catcagtctg
     6961 atataaacgt ccaaacgatc ggacagtcgt aaaaataaag gggatttaag attcataacc
     7021 ctctttcata taagtcttac acccttggaa aactaatcca taaacttcta gttccaataa
     7081 ccaatttgaa ttattgtcgt aaaatcttat tatttttaga ccatcttctt ggttctttat
     7141 ttctacttgg catatatttc tattgttatg cgaccaattt tgacatttca ttcctttaat
     7201 aaagctattt cttaattcaa tttcaactgt atcataatct tttagccaca gcccccattt
     7261 ttcgatttct acagaaggct ttgtacttgt tcggatagtt aaaatacaat tagacaagtc
     7321 agttgaaaaa gagactagtg aaatatccat tatgccacta ggatatattt gttttagaaa
     7381 acgattccta cttattattt ctattatatt catatttctt actaaaaatt atagtgtccg
     7441 tgagtaccat cgacatgtcc tgtatctaaa ttggagtctc ttatattaaa atgtggttta
     7501 gatgcaggtt gccgcacagc ttgaaaaaac ggtattttat ccgataaagc gtttcagttc
     7561 gggcagaaaa tactcggtca gcagcatttc ctcgccttga tgggaaaacc gagaacgccg
     7621 cgcggcaaag taccgtccgc atccttcact ggaaacggca aactcaaacg ccgaacgcgc
     7681 cccttccaaa tcggcttgaa acagacgctc gcccaaaggg cgtgtgccgc agtccaaaat
     7741 gttttgccaa aacgccgaac cgacgctgca cgcgcttcgt gcctgcacaa cagcagtgcc
     7801 gtccagtttc agaagcacat cgcgcaccct ccgccgtccg tattccgttt ccgcttcgcc
     7861 cagtttcagc agttccaccg aaaatgtatg cggcaaggcg cacaatgcgg cggtcagcga
     7921 ccgggctttc aataaccggc tcatcggcag gtcgatgccg tctgaaacgg gggcgggcaa
     7981 gtcgggcagc catttcccaa ataggtgttc catatctttc ccaatcttta taccgcgcct
     8041 gtttttgcca actccatcca ttccgcttcg ggaaaatcgg ctttcagacg gcattgcaga
     8101 tagttcaggc tgtcttgggc gacgtgtgcg tcgcggctgt cgtggatgtg gttaaacacg
     8161 aggctgtgca ggcgaatgcc gtattgtttg agcacgacga aactgagcag ggtgtgatta
     8221 atgcttccca gccgtccgct ggtaacaagg atgacgggat aagcctgttg ctgaatatga
     8281 tcaatggtta acagcttttc cgtcagtggt accatcaacc cgcccgcgcc ttcgaccaaa
     8341 acgacttcgt actgcgccgc caattcttgt gtggcggtgc ggattttgtc caagtccaaa
     8401 cccctgccgt ccagtcgggc ggcaaggtgg ggcgaggcgg gatggctgaa gatttcaggc
     8461 atagtcagac gctgttcatc agcttcctgc atgggtatgc ccatgatttt acggtggacg
     8521 gcgatgtctt cggcgatgtc ttggcaaccg gtttgcacgg gcttttgcgt aatcacgctt
     8581 ttgccctgct gcaacaattg ttttgccaac ataccggtgg cgacggtttt gccgatgtcc
     8641 gtgtctatac cgctgacgaa gtaaacgcct ttcatttgct gtgttccttc aagatttgca
     8701 cggttttatc ggcaagtttg gtcaaaatgc cgtctgaaat gatatagggc ggcatcagat
     8761 acaccagcct gccgaacggg cgtacccaaa tgccctgcgc cacgcaatcc gcttgaaaac
     8821 gcgccatatc cacgcctttt tccaactcta tcacgccgat agcacccaaa acgcgcacgt
     8881 ctttcacgcc gcgaatgtcc cacgcggctt tcagacggcc ttttaagatg ctttcgatac
     8941 ggcggatatt tgcctgccag tcttgggata aaagcagctt gaccgaagca caggcaacgg
     9001 cacacgccag cgggttcgcc ataaatgtcg ggccgtgcat aaacacgccc gcttcgccgc
     9061 gtgaaatcgt ttcggtaact ttttgcgaag tgattgccgc cgccagcgtc atatagccgc
     9121 cgctcaaacc cttgccgata cacataatat ccggcacgac ctccgcgtgt tcgcaggcaa
     9181 acatcttgcc cgtgcgcccg aatccggtgg cgatttcgtc aaaaatcaac acgatatcaa
     9241 attcgtcgca caaatcgcgc aagccgcgaa gatactgcgg atgataaaaa tacatgccgc
     9301 ccgcgccttg cacgaccggt tctaaaataa aggcggcaat atccacatga tgcgcttcaa
     9361 acaaggcgcg gacaggctgc aaatccgccc cgtcccattc atcgtcgaaa cggtttttcg
     9421 gattatcgac aaaataacgc tgcggcaacg cgctgccgaa aatatggtgc atccccgttt
     9481 ccggatcaca aacggacatc gcgttccaag tatcgccgtg atacccgcgc cgtaccgtcg
     9541 caatattctg cttcgccgtc agaccccgcg cctgctggta ttgcaccgcc attttcaacg
     9601 cgacttctac cgaaaccgaa cccgaatccg cataaaaaat acggtccagc ccctgcggca
     9661 aaatcccgac caacaacttg cccagctcca ccgccggctc gtgcgtcagg ccgccgaaca
     9721 tcacgtgcgc catttgtttc atctgattct caaccgcctg attcaaaacg ggatgattgt
     9781 aaccgtgtat cgcacaccac caagacgaca tcccgtcaat cagccgcgtg ccgtccgcca
     9841 attcgataaa caccccttct gcacgtttga caggataaac gggaagcgga tcggtcatgg
     9901 aagtataggg atggagcaga tgggtacggt cgaaattaag taatgatgat gtgtgttgat
     9961 gttcagacgg cataagtttc tctcttttct tattactgca ttcaaacgca aaaagcgtat
    10021 tctactccga cagaccgctt cccacacctt tccatccgtt tcgggcgaaa aaccgcgaaa
    10081 caaatcgtcc gcaacataag cgcacaccgt ttcgcattcc tcaagcctga ttggaatcag
    10141 acgacccaac gcccaatacc gtttcacaca gcctccgccc aacgcttcaa tcaatcatag
    10201 ataaatacga tacagatcat agcaacagcc atcgcaacag cgttggcaaa atcaggggac
    10261 tccggcatag gcgcatagca tctgccgatg cacggctcct cattcggctc tatgaatacc
    10321 atacccatca caaaatccac tgctaaaacc aggcacggct tcttatactt atgatagatt
    10381 tccaccatcc tgtcccatat atcccaaaca ctcataccgt atatcccgca ggcaacaaat
    10441 tccgattgaa ggttacagcc ctattttata gtggattaaa tttatagtgg attaacaaaa
    10501 accagtacgg cgttgcctcg ccttgccgta ctggtttttg ttaatccact atattttcaa
    10561 accggaaaaa tgccgtctga accttcagac ggcattccat tcatatttat tcgtcttttt
    10621 tgtgttcgat ggcgtttcgg gaacgattgc cgccgtaagg ctcttcaacc gtatattctc
    10681 cctcgataat atcgtcatcg tggaaaaatc cctcttttct gcccgattgg ttcatgttga
    10741 aaaaattttc cgcacctcct gcctgcaaca ctgcccctcc cttaaacggc agcagcagca
    10801 ataccgccaa caccgaggat acgaatcccg gactcatcag acacaccgcc gccaccgtat
    10861 aacggatagg ccacaacatc tgataaacag ataccttccc actacttttt accgccgcgc
    10921 cagccaataa aagaccggac agccccgtat gcctgagcat cagcacaccg gcggcaaagg
    10981 ttgccgccat tagaaacagc gtccaaccgc cgcccagcca atcggcaacc cacacaatcg
    11041 acataatttc caaaaacagc agcaccaaaa aaccgatacc gaaaaatctc attgaccgtc
    11101 atccttatat ttaagtaaac agcaaaccgc ccgaacagga ttccaagcga gctgcctgta
    11161 aatgattaca aaaccatgtg cttcaagccg aaacaatgtg aaatcacgca ataattattt
    11221 ttagcgtgta aaacaaaacc ggctgcatac ctgcaaccgg tctcaaatca gcacaactcc
    11281 ttatccaaat cctccaacag gtctttcaga ccgtctccga tttcctgaag cgtaaccgga
    11341 cggttgccgt ccgaacccga ttccgcacct tctgcatacg gtgcaaacgt gctcttcagt
    11401 ttcaacagtt tcaccacatc caaacgggtg tagcgttctc cgccgtaacc gacaaccaca
    11461 ccgtgatcat gctgccattg cgcaaaacca taggggctga tttgcaacag tccgcacaac
    11521 tcgtccagcg tgaaatagcg ttttgcagga atcgtcaaat caacgttgtt tgtcatagta
    11581 gtgttccacc atgcctttga gtttctggct ggcatggaaa gttaccacgc ggcgggcggt
    11641 aatcggcact tcctcgccgg ttttcggatt acggcccggg cgttgcggct tgtcgcgcaa
    11701 ctggaaattt ccgaaaccgg aaattttgat ttcttcgccg cttgccaaag tgctgcggat
    11761 ttcttcaaaa aagagttcga caatttcttt ggcatcgttt ttggtgacgt tgctgacttt
    11821 atccaccaaa atatcggcca gttctgcttt agttagtgtc atatgattac cttctgttgt
    11881 aacagttgtg gattattacc aatttttatt taaaattcaa gcgaatattt attttttaac
    11941 tgcgaagccg cgcccctgcc tccgttgcca cgccaatcag ttttccaata agcggctcga
    12001 ccgcctcatc cgtcagcgtg ttttccatat cctgcaaaat cactttaacc gccacgctct
    12061 tcatcccttc gggcagaccc gtgccgcgat acacgtcaaa cacgctgatt tcctgtacca
    12121 acttgtttgc tgcgcctttc aagacaagca gtaaatcatc atgattcata acttccggca
    12181 tcacaaacgc caaatcgcgg cgcaccggct ggaatttcga tacggcccga tagcgcgttt
    12241 tcccgcattc caacacagcc gccatatcga tttcaaatac cagcggcgct tgcggcaggt
    12301 cgtatttttg cagccatttc ggatgcagtt cgccgacaaa gccgatgact ttgccgtctg
    12361 aaacgatatt ggcggcacgt ccgggatgca gggcgggatg tccggtttta acgaactcga
    12421 ccgctttgtt tttcaacaga ttttccacgt ccgccttgat gtcgtaaaaa tccgcattgc
    12481 gcgttttctc accccattgt tcgggcatcg ccgcgccgta ccacagaccg ccgatgcgtt
    12541 cgttttggac aaaccggccg tctgaacctt tgccgaacac gcaggcgatt tcaaacacgc
    12601 gcacgcggtt ttgcttgcgg ttcaggttgt tttgcaaaat ttccactaag ccgccaatca
    12661 acgtagaacg catcacggca tattgcgccg ccagcgggtt ttgcaggcgg atgggggcgg
    12721 cgttggcggc aaaatcgagt tcccactgct cgtcaacgaa ggcatagctg atcacttcgc
    12781 ggtagccgcg cgccgccatt tcgttgtaaa cggcaaaacg cggacggcgt gtttcgggca
    12841 gttccgacat tttcagacgg cctgacgtgt aatcgtcggg gatgttttca tagccgtaaa
    12901 cgcgtccgat ttcttcaatc aaatcagcct caatttcgat gtcgaaacgg aagctcggcg
    12961 cggtaatgcg gaagccttcc gccgttttct caggctgcag acccaagtgt tgcaaaatgg
    13021 tttccacctg ttcggcagga atgtccacgc ccaacacggt tttcagacga cccaaacgca
    13081 atccgacctg cttggcttcg ggcaatttac cttgtgcttc caccatctcg cctgccgcac
    13141 cgccgcaaat ctgcaaaacc aattcggtgg cgcgttcaat ggcatccgcc tgcaaacggt
    13201 aatccacgcc gcgctcgaag cggaacgacg aatccgaacc gaaaccgtat tggcgcgatt
    13261 tgccgacgat gatttcgggc tcaaaccaag ccgcttccag cacgatattt tgcgtgccgt
    13321 ctgaaaccgc gctcgccgcg ccacccatca agccggccaa gctcaacgca cctttttcat
    13381 cagcgaccac cagtgtattg tcagccaaag taaccgtctt ctcattcaga cacgccaaag
    13441 tctcgccatt ttgagcacgg cggacaatca aactgcccga cagcttatca gcatcgaaaa
    13501 catgcatagg ctgaccgatt tccagcatca catagttgcc aatgtccacc aatgcggaaa
    13561 tgctgcggat accgctgcgt tccaaacgtt gtttcatcca atcaggagta gccgctttcg
    13621 cgttgacatt ttcaataacg cggctgataa aacggccgca gtcagccggt gcatcaatac
    13681 ggacagcctg ttttttctca ctgccgatag gggccgtctg aatttcaaca ggcgtaaacg
    13741 cacattgagt caacgcagaa acctcacgcg caatgccctt aacactcaag caatcagcgc
    13801 ggttaggcgt aattttcaac gtaaacagca tatcgtccaa atccaagtat tcgcggatgt
    13861 tggtaccgac aggcgcatct tcaggcagaa tgtgcaggcc gtctacacca tcatcaggca
    13921 aacccagttc attggtcgaa cacagcatac cgtttgatgg tacgccgcgc attttagtcg
    13981 gcttaatttt aaaattgccc ggcaaaacgg caaccggcaa cgaacacggc actttaatgc
    14041 ccggcttgac attcggcgca ccacaaacaa tctgaaccaa ctcgcccgta ccggcatcaa
    14101 cttgagtaac gttcaaacgg tctgcatcag gatgtttttc aacagatttt acttcggcaa
    14161 caaccacgcc actgaaagca ggggcggcag tatcgatttc ttccacttcc aaaccggcca
    14221 tggtcaaaag atgttccagt ttatcggcag aaagatcagg attggcttgg gttttcagcc
    14281 atgagtagga gaattgcatg ttatgttctc aatcaattaa ttaacttttg ttttatcaat
    14341 tttcgttcag acagtctgtt ttagaattct ggttaatgtt gatatttact caactctgaa
    14401 aaatattttc tctgtgaaat tttaaaaatt ccagacttgg gtttagtctt tcaggcagtc
    14461 ttatcatccg attttcatat ggtttaaata aatcatcaaa tccttctgtt tttgccagta
    14521 gtggagaaaa tataattttg aaattttcat ctattcctaa caacccccta tcaaaagctt
    14581 tgtcatgtaa cgcattcaaa catagcccat tccttgggtt taaacggtta tccttatctt
    14641 ccctccaggg tttgatatga ctagcaacta acaattcagg ttgttttaat ccagtaatac
    14701 aacattgatt attgtatgct gccaaaacac tggaacggaa aaagccctga ttgacgcgta
    14761 ctgatattct tgcaaacctt tcccttccct ttggaatctc aggtgattct atttcaatat
    14821 tactattctc atcatttgat tggtattggg aaatgactcc ctcaaattct ttattaagtt
    14881 gctcccaatc tcctgaaaac tcattccata cgacaacatc cattttactg ccattactta
    14941 atcccgaaat atttttgact ttcagcgtag gatctaaacg acccaaattt tctatcttca
    15001 taccgagtgc ggacggtgtc ctgcccagta tttcagcata ttcttgaata acaggattgt
    15061 ccttactaac cttttgaaaa ggaatgatgt aataaagata aagggcaacc agggtttcat
    15121 ctctagtcca ttttttcatt tttatcagct atctttctga ttttccataa ggaaatcgta
    15181 caatacagat tttcttattc cctgaacata ttgctgtcca tctagtacta atttgtatag
    15241 gtatacatca taatctttta actccttctc gcttgccaat aaccagtcgt ctatttctga
    15301 agaaaaatag tcaataattt cttgtacctg tttatcagat aaattattta tatttatttt
    15361 attattcaaa atataatcag aaagcctcat atttctaaaa tctttatttt gataaggtaa
    15421 acccacacag cacatcaaat tgcttcggaa tgtattgttt gatttcttcc aaagtaatat
    15481 cgccaaacgg cacttctccg aaattggctt cgtacgtcag tttggctttt tcgtcccact
    15541 cgctcgaaaa cacgtattcc ccgcccaagt tctgcatcgc gatgcggaag cccccgatac
    15601 ctgcgaacaa gtcgataaag gtaaatttgg gtttcaaggc cgtctgaaaa gtatcgttgt
    15661 tttcaaaaag gcggtattgt gctgcttttt cttttaaccg ttcatcctgc ccatcttcct
    15721 taatcttgtt tttgtaaatc aattcatctt gtttggatga tttctttgac tttttcggca
    15781 cagcaggttg tatttttgac gacatcgtta ctccaaaaac gtaaaaccgt ccagccctcg
    15841 gctttcagac ggcctgttac ttctatatcc ctttggatat tgcgctcaat tttggcaatc
    15901 caaaactcac gatttccctt tattaccgct tttttctgtt cccaatcctt accgtgccag
    15961 aattccccat cgacaaatac ggcaacttta tattttttaa atgaaaaatc aggttttcca
    16021 aaaatgcttc cgctattttt ccgatacctg agccccaaag cccacattgc cttcgccaac
    16081 acaagctcgg gtttcgtccc tgtacttttg ttggactgca tacatttttt gcgctgctcc
    16141 ggggttaatt tatccattgg gtttatttga gatttattca aactgcttca agaagttcaa
    16201 atcgttatcg aagaacaagc gcaggtcatt gacgttgtag cgcaacatgg caaagcggtc
    16261 gagaccaata ccgaaagcga aaccggtata tttttcaggg tcgatattga catttttcaa
    16321 cacattagga tgtaccatac cgcaaccgcc tacttccaac catttgccgt tttctcccat
    16381 gatgtcgatt tcggcagaag gctcggtaaa tgggaagaaa gacggacgga aacgcacttg
    16441 taggtcatcg cgttcaaaga agcgacggat aaagtcagtg aacactgctt ttaagtcggc
    16501 aaaagttacg ccctcttcta cccacaaacc ctcggcctga tggaacatag gcgagtgcgt
    16561 ggcatcgctg tccacacggt aaacgcggcc gggggcgata atgcggatgg gcggctcttt
    16621 tttatcgagc atatagcgga tttgaatcgg ggaagtgtgc gtacgcaaaa catcgccgtt
    16681 ttcaacgtaa aacgtatcct gcatcgcacg ggcaggatgg tttgcaggga tgttcagggc
    16741 ttggaaattg tgaaaatcgt cttcgatttc aggcccgtcc gccacttcga aacccattcc
    16801 gtgaaagagt tcgaccacac gttgcaaggt cagggttacg ggatgcaggc tgccgccttc
    16861 ctgagcgcgt ccgggcaggg taatgtcgag ggcttcggcg gcaagtcggg cttgcagctt
    16921 ggcttcgttg agggcatcgc gtttggcatt aaaagccgtc tgaaaccggt ttttgcattc
    16981 attgatatgc gcacctatgg ttttgcgctc ttcaggcgac atttgcccca aagttttcag
    17041 aagtccggtc aactcgccgg ttttgccaag ataacgggct ttgatttgtt ctagagcgtt
    17101 gaagtcttgc gcagcttcta ctgcggcaat gccttctgca acgatacggt ttacattttc
    17161 cataatatca agcctgtcaa ttaatgatat gccacctgaa acaacgaggc cgcctgtata
    17221 gcctgacata tcagactctt cagacgacct tattttcttc acggccaatc cattggataa
    17281 attttctgta ttttaacgca aatttattta aaaaaataga ctttaaagaa agaaaatcaa
    17341 tcacatagaa tttccgcaaa caaagaaaaa aggaagccgc agcttccttt tcaatttttt
    17401 ggattaagca gccaaagcag ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc
    17461 gaacacggcc aaatcagcca atactttgcg gtcgatttcg atagaggcgc gtttcagacc
    17521 gttcataaat ttgctgtaag acaacccgtt ttcacgcgca cctgcattga tacgaacaat
    17581 ccacaattga cggaattggc gtttgcgttg gcggcggtca cggtatgcgt attgaccagc
    17641 tttcattacc gcctgcttgg caacgcggta aacgttttta cgacgaccac gatagccttt
    17701 ggctaacgcg aagatttttt ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg
    17761 catattctaa actccttaag cgtagggtaa cattttagca acagaagcca aatcgcgatc
    17821 atttaccata gaggtaccgc gcagttggcg tttgtttttg gtggtctttt tagtcaagat
    17881 gtggcgtttg aacgcatgag cgcgtttcac accgccgtta cccagtactt taaagcgttt
    17941 tttcgcgcta gacttggttt tcattttagg catgggaaaa ctccattcgt tatcggataa
    18001 ggcattaggg ggttttaaaa catcggtttc aaacacttga acccacaatg ccacggtttt
    18061 ttgccgcaat tgaaaactta ctccgaagcg gcaatcggag taagcggaaa attatagctt
    18121 tattttttct tcggcgcaat catcatcacc atttgacggc cttccatttt gggaaaggac
    18181 tcgatttgcg ccacttcagc caaatcttct tttacacgtt ccaaaagttg cgcgccgagt
    18241 tgctggtgag ccatttcacg gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg
    18301 gcaaggaagc ggttgatgtt gcgcatcttg atttgataat cgccctcatc tgtacccgga
    18361 cggaatttga tttccttaat ttgcacctgc ttttggtttt tcttggcttc gtcgcgcttc
    18421 ttggcttgtt ggtatttgta tttaccgtaa tccatcagtt tgcacacagg aggtttagca
    18481 gttggggaaa tctctaccaa atcgacatcc tgcccttcgg ccatagccaa agcttcacga
    18541 actgaaacga caccaagctg ttcgcctgac tcactgatta aacgcacttc tttggcggta
    18601 atttcaccgt tgattcgtgc ttcgcgttct tgagcgatga tgaatactcc tataaaaatt
    18661 aatgattgac gagggcatca gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat
    18721 ccaaagaacc caagtcttcc gctttgcggc gtaccgcaac tttgttttct tgtttctctt
    18781 tatcgccgat aacgatttga taagggaaac ggtattggct gttgtcgcgg attttgtaac
    18841 cgattttttc attacgcaaa tccaactcgg cgcggaagcc tgccgcctgc aatttggcag
    18901 caacttcccg acaataatct gcctgatttt cggtgatgtt cataatcacc atttgaaccg
    18961 gtgcaagcca taacgggaaa gagcctgcat ggttctcaat cagaatgccg acaaaccgct
    19021 ccaaagaacc taaaatggcg cgatgcaaca taacaggccg cgctcggtcg ttgttttctg
    19081 ttacatattc tgcatccaag cgttccggca agacaaaatc cagttgtaat gtaccgcatt
    19141 gccaagaacg acccaaggca tctttgacgt gatattcgat tttaggcccg taaaacgcac
    19201 cctcgcccgg caattcgccc cattccacgc cgcaggcagt caatgcctcg cgcaaaccct
    19261 gctctgcctt atcccacacg tcgtctgaac ctgcgcgttt ttcagggcga agggaaagcc
    19321 tgacggatac atcatggaaa ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg
    19381 cacgagcctc gctgacgatt tgatcttcgg tacaaaaaat atgcgcatca tcctgcacaa
    19441 aaccgcgaac cctcatcaga ccgtgcagtg cgccgctcgg ctcgttgcgg tggcaagaac
    19501 cgaattccgc caaacgcatc ggcaaatctc gatatgaacg caaaccgttg ttgaaaattt
    19561 gaacatgacc cggacagttc atcggtttaa ccgcatattc gcgtttttcc gaactggtta
    19621 cgaacatatt gtctttgtag ttgtcccagt ggccggattt ttcccaaaag gttttatcca
    19681 taatttgagg cgttttgacc tctttataac cggcggcgtt cagctcttta cgcatatgct
    19741 gctcgatagt ctgccacaaa gcccaacctt tgggatgcca aaacaccata cccggcgctt
    19801 cgtcctgcaa gtggaacaaa tccagttgct tgcccaattt gcggtggtca cgtttttctg
    19861 cttcttcaat gcgttggata tagtctttta attcgtcttt cgtcgcccaa gcagtgccat
    19921 aaatacgttg cagcatttca ttattgctgt cgccgcgcca atatgcgcct gccagcttgg
    19981 tcagcttgaa gtttttcagg aagcgggtgt tcggaacgtg cggaccgcgg cacatatcga
    20041 catattcctg atgatgatac atacccattg cttcaacttc gggcatatct tcaatcaggc
    20101 gcagtttgta ttcttcgccg cgctcttgaa aaattttaat cgcctccgca cgcggagtca
    20161 tgattttgac cacatcataa tcttgggcaa tcaattcttt catacgcgct tcaatggcgg
    20221 caacatcttc cggtgtaaac ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa
    20281 tgacggggcc gataaccatt tttgcattag gatagagttg cttgacggca tgcccgacaa
    20341 gatgtgcgca ggaatggcgg atgatttcga tgccttcctg atctttcgga gtaatgattt
    20401 gaacagcaga atcctcaaca atcgggtcgc acgcatcgac caatttgccg tttaccttgc
    20461 ctgccaccgc cgccttcgcc aaaccggcac cgatagacgc agcaatttga gccacggtaa
    20521 cgggggattc gtattggcgg actgaacagt ccggcaaggt aatattcaac atcaaacgct
    20581 ccgaaagatt aaaaaataac ggcataaatg ccgttatggg aatttggtag gcacgattgg
    20641 attcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga gctacgtgcc
    20701 ttcaaagaat ttttgtattt tatcgggtcg ttttaatttt tgcaagagag atgtgcattt
    20761 ctttatgcaa cattgtggca agatttgggg ataccgtctg aaaaaccgtg ggaattcatt
    20821 tataatgccg tcttttaaaa tatatttcga cccctgtcgc ccaacttaca ttcaatctta
    20881 tgcttaaatt tactttacac aaaaaagacg gtctcgcccg acgcggcacg ctggagttga
    20941 accacggaaa aatcgaaacg ccggttttca tgccggtcgg tacttacggt tcggtcaaag
    21001 cgatgaaccc gcaaaacctg cacgacatca aggcgcaaat tattttgggc aacacttatc
    21061 atttgtggct gcgtccgggt ttggaggtcg tcgaacagtt tggagggctt cacggtttta
    21121 tcggctggga caaaccgatt ctgaccgact cgggcggttt tcaggttttt tctttgtcgg
    21181 atatgcgcaa gctgaccgaa gaaggctgta cgtttaaaag cccgattaac ggcgacaagc
    21241 tgtttttatc acctgaaatt tccatgaaaa tccaaacggt tttgaactcg gatatcgcga
    21301 tgcagttgga cgaatgcacg ccgggcgaaa caacgcgcga acaggcgcga aaatccctgc
    21361 aaatgagctt gcgctgggcg gaacggagca aaaaagcctt tgaagatttg aaaaacccga
    21421 acgcgctgtt cggcatcgtg caaggcgcga tgtatgagga tttgcgcgaa gaatctttgc
    21481 gcggtttgga ggaatttgat tttcccggcc ttgccgtcgg cggtttgtcc gtcggcgaac
    21541 ccaagcccga aatgtaccgt atgctgcacg ccgtcggccc gattttgccg gaacacaagc
    21601 cgcattacct gatgggcgta ggcacgccgg aagacctcgt gtacggcgtg gcgcacggta
    21661 tcgatatgtt cgactgcgtg atgcccaccc gcaatgcgcg caacggctgg ctgtttaccc
    21721 gtttcggcga tttgaaaatc aaaaacgcca aacacaagct cgacaagcgt ccgatagacg
    21781 aaagctgcac ctgctacgcc tgccaaaatt tcagccgcgc ctacctgcac catctgcacc
    21841 gcgccggcga aatcttgggc gcgcaattga acaccatcca caacctgcat ttctaccaag
    21901 tcattatggc ggaaatgcgc gaagccgtcg aacaaggcaa atttgccgac tggcaggcgc
    21961 aattccacga aaaccgtgcg cgcggcgtgg actgattccc ggcaatgccg tctgaagccg
    22021 cttcagacgg cattttgccg ccttacccta cctgcactat ttttcagaaa ccgatcatgc
    22081 ttccattcct tccagaaccc tccctttcct atacgcaaca aaaccgtacc gccgttttgc
    22141 tgttgaacct cggcacaccc gacgcaccga ccgcacaggc ggtacgccct tatctgaaat
    22201 cctttctgac cgaccggcgc atcgtcgaac tgcccaaatg gctgtggtat cccattctgc
    22261 atggcctggt actgacgttc cgccccaaaa aaagcgcgca cgcctatgaa aaaatctggt
    22321 ttaaggaagg ttcgccccta gaagtttaca cagcacgcca agctgccgcg cttgccgaac
    22381 gtatgcccga cctcatcgtc cgccacgcca tgacttacgg caatccttcg atagcggatg
    22441 tattggcgga gctgaaatcc caaggtgtag gcagattgct tgcgattccc ctgtatccgc
    22501 aatatgccgc ttccagcagc ggagcggcgg tagataaagt gtgcgaacaa ctgctgctgc
    22561 aacgcaacca aatgagcgtc cgcaccattt cgcgctttta tgatgatgcc ggctacattg
    22621 atgcgatgaa aaaccacatc ctccgatatt gggcggaaca cgggcgtggg aaaaaactga
    22681 tgttgagttt tcacggcgta ccgcagaagc actacgacct cggcgacccc tatcccgacg
    22741 agtgccgcca caccgccaaa ctgcttgccg aagcactgga actgaccgaa gacgaatata
    22801 ccgtatcgtt ccaaagccaa ttcggcaggg caaaatgggt tacaccgagc acgcaggatt
    22861 tgttcggcaa actgcccaaa cagggggtaa ccgagctgga cgtattctgc ccgggctttt
    22921 tggcagactg cttggaaacc atggaagaaa tcgccctgat ggggcgggaa cagttttatg
    22981 aagcgggcgg aaaaaactac cgctacatcc cctgcctcaa cgacaacccc gactggatag
    23041 acgcactcgt cgcacttgcc gaagaaaacc ttggcggctg gcgttgaaac tgtttgagcc
    23101 atgtctaaaa tgccgtctga acgccgtgtt cagacggcat tttcccgcta ctttagcgta
    23161 tgatggcatc tatgccggac agcacatcaa gtgcaacctt actaccgatt cggcaaaaaa
    23221 acctttcacc cgctacgcaa aatcaacaac aaggtttcaa acattaagga aacgccaatg
    23281 aacacaaccc gactgccgac cgccttcatc ttgtgctgcc tctgcgccgc cgcttctgcc
    23341 gccgacaaca gcatcatgac aaaagggcaa aaagtgtacg aatccaactg catcgcctgc
    23401 cacggcaaga aaggggaagg gcgcggcact gcgtttcctc cgcttttccg gtcggactat
    23461 attatgaaca aaccgcacgt cctgctgcac agcatggtca aaggcatcaa cggtacaatc
    23521 aaagtcaacg gcaaaaccta caacggattc atgcccgcaa ccgccatcag cgatgcggac
    23581 attgccgccg tcgccactta tatcatgaac gcctttgaca acggcggcgg aagcgttacc
    23641 gaaaaagacg taaaacaggc aaaaggcaaa aaaaaccaaa cagacaaaat gccgtccgaa
    23701 accggcaatc cggcttcaga cggcattcaa accaaacctt tctaacgcgg tactttcagc
    23761 cccatagacc cgatattgcc gccaccgtca acctccttcg cggtcagcat aaagccgccg
    23821 atgtcaatac ggtcgccctc gaccggctcc tgactgtcgg aacgcttatc gaaaagctcg
    23881 gcaaggctca aaccctcttc gcccgcttcc agcttcaaac cgtaagcaag tgccaaatcg
    23941 cccgaacgtg ctgccggcga aacgacaaac tcaccgaaga aatcaaaatt ttcacggacg
    24001 ccgatacccg tctcggtaaa gtatttcgcc attttatcga ccttgccgtc ggccaggatg
    24061 taccacgcca aatcctccgc ctgaagcacg gtgtcggact gcatttcgat tcggctgccg
    24121 ttgcgtatca atgcgaaaca gcgtgcgtca aacgaatcgg aaatcggttc gaccgtatcg
    24181 ggatggtgtc cctccgcttc agattcggca accactttaa acgccgacat tctgacaatt
    24241 tccttttccg ccagccaaat atcatgcgta tctttcggtt cgggcttgtt gggcatggca
    24301 accttcaaca gccgcgccat cacaggaatc gtcgttccct gaatcagcag cgacagcacc
    24361 accacggcaa acgccacatc aaacagcagg tgcgaattgg gaacgcccat caccagcggc
    24421 atcatcgcca gcgaaatcgg tacggctcct cgcaagccca accaactgat atacgccttt
    24481 tcacgcaggc tgtaattgaa tttccacaaa ccgccgaaca ctgccagcgg acgcgcgacc
    24541 agcatcagga acgccgcaat cgccaaggct tccgccgccc tgtccaacac gccggcggga
    24601 gaaaccagca gaccgagcat gacgaacaaa gttgcctgcg ccagccaagc caaaccgtcc
    24661 atcacacgca aaacgtgttc cgtcgcacgg ttgcgctggt taccgacaat gatgccggca
    24721 aggtaaaccg ccaaaaagcc gctgccgcct atggtattgg taaacgcaaa cacaagcagc
    24781 ccgcccgaca caatcatcag cgcgtacaga ccttccgcaa gattcagacg gcgtaccagc
    24841 tttgccaata tctttccgcc cgcccaaccc gtcagcagac cgaaaccgat ttgcagcgca
    24901 agcatccgga caaacgctgc cgcacccgat tccgccggct gcataatcat ggtaatcagt
    24961 gccgtaacca aaaaaaccgc catcgggtcg ttcgcacccg attcgatttc caaagtcgcc
    25021 tgcacccgtt cgttcaaacg cacgccgctg ttgcgcaaaa ggctgaatac cgcgccggca
    25081 tcggtcgaac cgacaatcgc cgccatcagc acgccgaact tccaatccaa accgagataa
    25141 aaagttgcaa acagtcccag cggaagcacg gtggcaaaca cgccccacga agcgagtacc
    25201 gacgcgggct tcaacgcaat ccgaaaactg gaaagctgcg tccgcaaacc gccgtcgagc
    25261 agaataaccg ccaaagcaag ctggctgatg aaattcgcca tcacgacatt attgaaggca
    25321 atgccgccaa cgccttcctc gcccgccagc atgccgacgg caagaaacac cagcagcaaa
    25381 ggcattccca aacgtgcgga caaagtggtc gaaaccacgc tcaggaacaa cagtgcgcca
    25441 cccagcagaa acaggctgtt catccaatcc acgccaacgg ctccccctta aaattcatga
    25501 aattagaatc gaaagattgt aacacggcat tttcttctca cgaaacttta tttcatgtcc
    25561 gccaccgcct tcagacggca ttttcctgtt tcatgccgcc tgataccgcc caaacccgcg
    25621 ttctcctata taatgtcgga ctttgcgccg catttcagga ttttttatgg catttgcctc
    25681 actatttact ctgccggact acatcaccgc cgttttggac gatgttgccc tgatgaccaa
    25741 agccgccgca aagaaaaccg ccggcgtggc cggcgacgat ttggcaccca atgccaaccg
    25801 ggttacaggc gtatcggcag aacgcgagtt gccgattgtt tggccggtgg cgaaaggttc
    25861 ttttgccaac aagccggttt tggtgtctgc ggctctgctg ctttccgcct tcctgccgca
    25921 actcattacg ccgcttttga cggcaggcgg gatttatttg tgctttgagg gcgtggaaaa
    25981 gctgctgcac aagtttctgc accgacatga cgcgcacgat gacggcggcg aaacggcggt
    26041 taacgagcag gctaaaatca aaggtgcaat ccgtaccgac tttatcctgt ccgcagaaat
    26101 catcatcatc gcgctgtgcg tggtcgaaca atacagcctg atgacgcgct cgctcgtgat
    26161 ggcggcaatc ggcatcggca tgactgtttt ggtgtacgga atcgtcgccg tcatcgtcaa
    26221 actcgacgac ttgggcatgc tgctgatgcg ccgcccacaa cctcggcctt ttgcacgatt
    26281 tcctgcacgc gcaacactgg aacgctggct ggacagagta ttccgccaac ctcgttgtcg
    26341 ggccgctttc cggttcgatt gcctgcgccc cgccttgtcg ctgatgaatc gtttcggcag
    26401 gcattgattc ctttttcaaa taccgatgcc gtctgaaaga cgttcagacg gcatattccg
    26461 aacagacaga cgaatatgtt ttccaaactg gacaaatact ggcagcaccc cgccctctac
    26521 tggcctttgc tcatcctttt tgccgccgcc acccccttcg ccttcgcacc ctactaccac
    26581 ttttggctga tgcccttgat tttcggcgcc ttcgtccgcc tcatcgaact gcgtccgcgt
    26641 tttgccgcct cttccgccta cctgttcggt ctgaccgcat acacggcaca gttctactgg
    26701 atacacactg ccctgcacga cgtttccggc ctgcccgacc tctatgccgt accgctgacc
    26761 ttcctgctgc ccgcctacct tgccctttat ccggcactgt gtttctggct gtggaaaaaa
    26821 tttaccctgc cccagggcat aaagaccggt ctggtactgc ccatcctgtg gacgctgacc
    26881 gagtttgccc gcgagcgttt cctgaccgga ttcggctggg gcgcaatcgg ctactcccaa
    26941 atcaccccgg acagcccgct ggcgggtttc gcccccttgg gcggcatcca catggttaca
    27001 ctggcaaccg cctttctcgg tgtctggctg gttttggcga tcgacaacac cgcacgttcg
    27061 ggcaaacgcc tgcttccggc cattctgatt gccgccctgc ttgccgcagg ctacaccgcc
    27121 caacaaaccg acttcacccg ccccgacggc agccgcagca ccgtcgccct gcttcagggc
    27181 aacatcgccc aaaccctcaa atggcgtgaa gaccaagtca tcccgaccat acagaaatat
    27241 tacgaacagg tcggcaaaac cactgccgac atcgtcatcc tgcccgaaac cgccattccc
    27301 atcatgcgcc aaaacctgcc ggaaaacata ctggcgcaat tcgccgaaca ggcgcaaaac
    27361 aacggcagcg cgctcgccgt cggcatcagc caatacactt cggacggcaa cggttacgaa
    27421 aacgccgtca tcaacctgac cggctatcag gaaaacaatc aggacggcat cccttactac
    27481 gccaaaaacc acctcgtccc cttcggcgaa tacaaaccgc tgcccctcct gaccacaccg
    27541 ctttacaaaa tgatggacat gcccctttcc gacttccgca aaggcggcgg caagcaatcc
    27601 gccatgatga tgaaaaacca aaaaatcgcc ttcaacatct gctacgaaga cggattcggc
    27661 gacgaactga ttgccgccgc caaagacgcc acactgcttg ccaatgtcag caatatggcg
    27721 tggtatggaa aatccaacgc catgtaccag cacctccaac aatcgcaggc gcgggctatg
    27781 gaactcggac gctatatggt ccgcgccacc aacaccggcg caaccgccat catctccccc
    27841 aaaggcaaca tcatcgccca agcccaaccc gatacggaaa ccgtattaga aggacacatc
    27901 aaaggctaca tcggcgaaac gccctatatg aaaaccggca gttcatggtg gttgatgggc
    27961 atattgaccc tagccgcact gattcttttc atcttccgaa acaaagaaca ctgatacctg
    28021 cacagtccgt ccggacgggc tgtctttttt caaaccggcc gtttacatac aaccacgtat
    28081 gtaatataat tctaaaatcg aattgagcta tcaggtttac aaaatcaatc aaacttgcct
    28141 tacaaaattt tcctgtcttt caaaccttag cgtatatctc ctgccaaaca tacaaaatac
    28201 aagccaagca gtgatatact gcatcccaac acaccaaacc gccgatacgc agtttcagcc
    28261 gaaaggaaat gccccaaatg aataacgctt tcgcattacc cgccatccaa agcggcaacg
    28321 gcagcctcga acaatacatc cacaccgtca acagcatccc gatgctgtcc caggaagaag
    28381 aaacccgcct cgccgaacgc cggataaagg gcgacctcaa cgccgccaaa caactcatcc
    28441 tgtcccacct gcgcgtcgtc gtttccatcg cgcgcggcta tgacggctac gggctgaatc
    28501 aggccgacct gattcaggaa ggaaatatcg gtctgatgaa ggcggtcaaa cgctacgagc
    28561 ccggtcgagg tgcacgcctg ttttcatttg ccgtgcattg gattaaagcg gaaattcacg
    28621 aattcatcct gaggaactgg cgccttgtcc gcgttgccac caccaaaccg caacgcaagc
    28681 tgtttttcaa cctgcgcagc atgcgtaaaa accttaatgt cctgtctccc aaagaggcac
    28741 aagacatcgc cgacgatttg ggtgtcaaac tttccgaagt tctggaaatg gaacagcgca
    28801 tgacgggaca cgacatcgcc atcatggcag acaacagcga cgacgaggac agtttcgccc
    28861 ccatcgactg gcttgccgac cacgattccg aaccgagccg acaactatcg aaacaggcgc
    28921 attacgccct gcaaaccgaa ggcctgcaaa acgccttggc gcaattggac gacaggagcc
    28981 gccgcattgt ggaaagccgc tggcttcaag acgacggcgg actgacgctg caccagcttg
    29041 ccgccgaata cggcgtatct gccgagcgca tccgccagat tgaagcgaaa gccatgcaga
    29101 aactgcgcgg tttcctgacc gaagaagccg aagcggtttg aaatatacac aataaaatac
    29161 ccgacaaaac aatttgtcgg gtattttatt tggtaaacat caatgcggtt tgtgcgtcgt
    29221 gccgtacacg attttatccg cccaagccat cgcaatcgcc aaaatcaaaa agcagacatg
    29281 caccagacac tgccacatca gctgcttttc cgacagattg gcggaattga taaatgtttg
    29341 gagcaaatgg atggatgaat accgataatc gacatcgaca gccttacctt caataccggt
    29401 gcattcacat ggctcaacca ctcgggccgg tcaggatggt cgtcgatacg caaccgggat
    29461 acgaacgact catacccgcc aatctgcacc atggtcagca aattcgcaat cataaccaca
    29521 tcaatcagat ttaaaacagc gagcatgatg gcgttttcgt ccgacacatc caaattcatg
    29581 accagatgct tcaacgattt caaaaactta taggcacaaa tcgcccgtac aacagtcagc
    29641 cccgcataaa tcggcacttg cagccaacgg cttgcaaaaa tcattttggc aaaaaaccca
    29701 tagcctgttc gggttttgga gattttgctt gatacatttc ccttccttgc agcaacaaaa
    29761 gcgcgtgatt ccaatctacc actgcgaaat cttcaatttt tcacacccga aaacgcttgc
    29821 cgccatacag atgtgatata ttgattccat tttaacgaac ttcggagaac gacatgagca
    29881 gcggaaatat ccgcatccac agcatgaccg gctttgccaa cgcggcggca gagtgcggca
    29941 gcaaacgcat caacctcgac atccgcgccg tcaaccaccg ctttttggac attcaaatca
    30001 ggatgcccga cgatttgcgc catttggaaa acggcatccg cgagcagatt tcatcccatc
    30061 tggcgcgcgg caaagtcgaa tgcaaaatcc aaattcaaga tgcggaaaac ggcaatcagt
    30121 ctttggaact gaatcgcgtt ctggtcggac agcttgccga aatcaacaaa aacctccgca
    30181 aacattatga tttggcaaaa ctgggcgttg ctgatatttt gcgctttccg ggcgtattgg
    30241 caagccaaaa ggaaaatacg gaagcattgg cgaaaaccat tgtcgaactg gccgacaaag
    30301 cactaaaaga ctttaccgcc gcacgtcggc gtgaagggaa aaaattgggc gaacacctgt
    30361 tacaacgcat taaaaacatg gaagaaatca tcgatgcttt gagcgaaatc ttccccgcgc
    30421 tggtggaagc gcataaagaa aaaatccgca cccgccttgc cgaagccgtc ggcagcatcg
    30481 acaacgacag gctgcaacag gaatttgccc tctttatcca aaaatccgac atcgacgaag
    30541 aattcagccg cctgcgcacc cacattgccg aagtgcgccg tatcgttacc gaacacaaag
    30601 gcagcagcgg caaacggttg gacttcctga tgcaggaact gaaccgcgaa gccaacactt
    30661 tgggcagcaa atccatcgct accgaatgca cccaagcctc ggtcgagctg aaagtcctga
    30721 tcgaacagat gcgcgaacag gtgcagaata tcgaataacc ctgcctttct ctccaaacat
    30781 gacaatgccg tctgaaactt ttttcagacg gcattcttga tcggctgaaa acctctgttg
    30841 caagcaattc aagattgttc tttcacatcc accaacctcg ttccggcaag gaagtcatac
    30901 agaaactgcc ggtcgggatt cagcagggcg aacccccacg gcaaaatcag ccaaaccaat
    30961 gccgcgccgg ccgcgccctt gggcggaatg ccgaggaagt ggcgcaatcc ggcataagca
    31021 agcataggaa tgaagacgat gaatatgcac gcccaaataa agcgcaggcg cagcaggtgc
    31081 aaagacggct gtatgccgtt aaggtcgcac aagccgattt tccatgtcct catcgccaag
    31141 gtctgcccct gaccgtgcca gttggcgcgg aaataaagcc accatgctcc cattatcagg
    31201 atactcgtta ccaatgcaga aaccgcgata gaaacgggat tcagaaaaat ggcggcaata
    31261 ccggcaagca atgctgccaa acaggtcgcc gcaccgacca gcagcatctc atacagtatg
    31321 gcggcaaagc ggcgtttgag cggggcggtt ttcagtatca tcatcttttc aatcggaata
    31381 tgccgtctga aacgccaaca gacttcagac ggcatttgtg caaagatgcc ttgcggcggt
    31441 taacggtgcg gacgtttctg accgcgcgac tgttttgccg ccatttgatt gatttcctga
    31501 cgtgtcgggc gttttttgaa catagcgtgc gcctcgtcca ccgaacattt ttgcgacagg
    31561 cagatcaggg taatcgcatc tttctggtgt tgtccgccgc tgtggtacat acggcgcagg
    31621 tttgcctcgt tgattctgcc tttttcggga tcgatgtcga attttttcag acgttcgata
    31681 aactggcgtt gctgtcggcg ggatgtccaa acaaagccgc ccgcagcggc ggcgacgatg
    31741 acggcggcaa gcaggatggc aagcggccag gaatcgacaa tcaatacgat aatgaggctt
    31801 aaaacggcgg tggtcagtaa aatcaggccg cccaagcgca gtttggtctt attgtccata
    31861 gggttgatgt tcctttattg aggtggtcgt gccgtctgaa atgttttcgg acggattggt
    31921 gaatactttt caggattaac ggtattttac agtattttcg gattttgggg attaccgctt
    31981 tattccgcca gcgacacgac aagctgtttg aataccgtcc atgcgtcgcc gtcttccgcg
    32041 cctttgatga ttcggtcgat ttgggcgcag gttttgagcg cgtcaagcag gcggacgacg
    32101 gaaatccgct tgaccgcaag cggtgcgagc gtctgtttgt cgccccaaag cctgaggctg
    32161 ttgcggacgg attggatgct ctgcccctgt ttcagggcag cggcaagccg aatcagcgtc
    32221 cgcacgtctt cggcaaccgc ccacagaagc agcaccggtt cttcgccttc ttcctccaac
    32281 ccgtccaaca gcctgcatac gcgcgggaca tcgcccttca tccacgcgcc tgccagttgg
    32341 aacgcgtcga agcgggcaac attggcaacg gcggtttgcg cctcatcgat attgacggca
    32401 tgccctttcg gatacagcag cgcgagcttg tcgatttcct gacgtgccgc caacagattg
    32461 ccttccacgc gctcggcaaa cagtgccaat gcgtctgcct cgatacccaa accgattttg
    32521 tccagccgtc cgcgtatcca ttggggcaaa gccgccgcac cgaccggttt ggcttcccac
    32581 acttccccct ttgccgccaa tgcggcaaac catttggact ggagccgggt tttctccagt
    32641 ttgggcagca aaaccagcgt taccgtatct tccggcagtc gggcggcaaa atcctgcaat
    32701 gcctcgccgc cgtttttgcc gggttttccg ttggggatat gcaattccaa cagtttcaaa
    32761 tcggcaaaca gacccgcatt gcctgcggtt tgcagcagtt cgttccaatc gaaagacgcg
    32821 tcggcggtat aggcttcgcg gttgagataa ccctgcttct tcgccgccgc cctcaatgcg
    32881 tccactgcct cgatacgcag cagttcttcc tcgccgtgga tgacgtacag aggtttcaaa
    32941 ggcgcgtccg tatcgatgcg tccgatatgt gccgccacgt cattccgcct tcagaaaggt
    33001 caggcggcgg acaatctgtt cggcaacatc ctgccgcatt tccgcccaca gggtttcttc
    33061 ttcttcctgt ttgcccaaaa tttcgttgtc ggcataatcc aaaatgcggc ggacggacac
    33121 ggtcatcggt ttgccgaccg gctcgccgcg tttcaatacc tgcgcttcaa ccgtcaatat
    33181 caaaagatat tcgttgatga ctgccgcacg ggtaacggta taggtttcct tgttttggga
    33241 aacgctgtct atacgcaggg tcatctgcgc gccggcagca tcgtccaccc tgcccgaagc
    33301 ctgatacagc gcggtttcca aaggaaactg caatgcctgt ccgccttcga tgtgccagct
    33361 ccggtaggtc agcggcggag aaatgccgtc tgcacctttc aggtggaaac cgcacgcgcc
    33421 cagcaccaag gctgcggcag taaggaatat tttgttcatc gcttgccttt ctcaaaaaca
    33481 taaagtcggt agattatatc agcggcgtgt tcagacggca ttgccgtcca gccaaatcag
    33541 gctcgccata cgccccgtcg ctccgtcgcg gcggtaggaa aagaaaatat cccgttccaa
    33601 aaccgtacaa tgcgtgccgc cgtacacgcc gcccacgcct tcgcgcctca gaatcaggcg
    33661 ggcgagcgcg tagaggtcgg caaggaattt gccgccgccg atgggttcaa atgcagcagc
    33721 ggcttcgggc atggacgtgc agaacgcatc aaacacgtcc tgcccgactt caaacgcatc
    33781 cgcactgatg gcagggccga gatacgccat aatttcgaca ggcggaacct tcatcgcggc
    33841 aatggtgttt tgcaatacgc cgcccgccaa accgctccag cccgcgtgtg cggcggcaac
    33901 cgccgtacct gccctgtcgc aaaatagaac gggcagacag tctgcagtca tcacggcaca
    33961 gacggcttta cccgtgtcgt ccacggaagc gtcggcatcg ggcgtgccgt ccaatgcttc
    34021 ggcagcattg acgacaatgg tgctgtggat ttgattgaga taggcaacgg gcaatccgac
    34081 ctgctgttgc acgatttcgc ggttgcggcg cacggtttca gggtcgtcgc cgacgtgcgt
    34141 accgaggttc aaactctgat acgcaccttg gctcacgccg ccgttgcgcg tggtaatcag
    34201 ggttttcaca ttggcgggcg cgggccaatc ggcggtcagg aagtttttgc ctttcggggc
    34261 gagatttagg gtttctgtga tggttttcat tggatattgg aataggtgtg cggcggaaag
    34321 ggttgattaa ctgccttcgg ggcaaatgga tgcttgtttt ttcgtcattc ccgcacaggc
    34381 gggaattcgg agattcggga ttgttttcaa acgttcgggt cggattcttg ccggtgcggg
    34441 aatgatgccc ttatatactt tctccgagct ttatatgctt caacagggga cggcacatca
    34501 agcaccgcac tgcgtgttgc ccgaacgagg ctgccctccg attagattct atcgctataa
    34561 acagacgggt ttcaaccgaa aaggaatggg gataaagtcc atttccgaca cctctcgggc
    34621 tataccgtct gaaaaccaat ctccactttc agacggcatt gtttaggcgc ggtcattttg
    34681 caaattaagg ttaaggcgtg tatgcttgcc ttcacttaaa aacaaacacg caacgcatta
    34741 ttatgaatat cctcagtaaa atcagcagct ttatcggaaa aacattttcc ctctgaacct
    34801 cgctctttgc cgccgccgct tttttcgcgc ccgacacctt caaatgggcg gggccttata
    34861 ttccttggct gttgggcatt attatgttcg gtatgggttt gacgctcaaa ccttccgact
    34921 tcgatatttt gttcaaacat cccaaagccg tcatcatcgg cgtaatcgca caattcgcca
    34981 ttatgccggc aaccgcctgg ctgccgtcca aactgttgaa cctgcctgcc gaaatcgcgg
    35041 tcggcgtgat tttggtcggc tgctgcccgg gcggtacggc ttccaatgtg atgacctatc
    35101 tggcgcgcgg caatgtggct ttgtcggttg ccgttacgtc tgtttccacc ctgatttccc
    35161 cattgctgac tcccgccatc ttcctgatgc ttgccggtga aatgctggaa atccaagcgg
    35221 ccggtatgtt gatgtccatc gtcaaaatgg ttttgctccc cattgttttg ggtttgattg
    35281 tccataaggt tttgggcagt aaaaccgaaa agccgaccga tgcgctgccg ctggtttccg
    35341 ttgccgccat cgtgctgatt atcggcgcgg tagtcggggc aagcaaaggc aagattatgg
    35401 aaagcggcct gctgattttt gcggttgtcg cactccgcaa cggcatcggc tacctgctcg
    35461 gcttctttgc cgccaaatgg accggcctgc cttatgatgc acaaaaaacg ctggccatcg
    35521 aagtcggtat gcaaaactcg ggcctggccg ccgcgcttgc cgccgcacac tttgccgccg
    35581 cgccggtcgt tgccgttccg ggcgcattgt tcagcgtgtg gcacaatatc tccggctcgc
    35641 tgctggcaac ttattgggcg gccaaagccg gtaaacataa aaaaccctaa acgcctttca
    35701 tcataatgcc gtctgaacgg ggcaaccgaa accggcggtt tctgcccggc cccttcagac
    35761 ggcctttgtt tgaaatcggc ggggacgatt gcccggctgc tccggttcgg ctgcctgcgt
    35821 tcgccccgcc tttcaagccg cctcactccc ttacataaac cacttcgaca tcgtagtcgt
    35881 cttcgttcca atcgtcatcg tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc
    35941 tcaaagacga atccaaaccg gcttcaagac ggaggacgga caacaaatga tatatgtcgt
    36001 ccggaatcgg tgcctcaaac gaaacggttt cgccgctttc cggatgggta aaactcaagc
    36061 ggtaggcgtg caacgcctga cgcgcgccca aacttttaac ggcttctttc accgtgtcgc
    36121 cgcacggatg gcgcgggttg ccgtaaaccg ggtcgccggc aagcggatgg ttggcctcgc
    36181 gcatatggac gcggatttgg tgcgtcctgc ccgttccgag cgagcattcg atgtagctgt
    36241 gggcaagata gcgttccaac actttgacgt gggttacggc aggcttgccg ccgaatttga
    36301 cggctgccat tttcaggcgg ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt
    36361 cgaaggggac gatgccgttg gcgacggcgc ggtagatgcg tttgaccgtg cgttcttgaa
    36421 gctgcctcac gagggaattt tgcgccggca gggttttggc aaccaccatc agcccgctgg
    36481 tttctttgtc caaacggtgt acgatgcccg cgcgcggaat ttgggacagc tcggggcaat
    36541 gcgccaacag gccgttgagc agcgtccccg tccagttgcc cgccgccgga tgcaccacca
    36601 gtccggccgg tttgttgacg acgatgacgg tatcgtcttc gtaaacaata tccaaatcca
    36661 taggctccgg aacaaacgcc agattttcct cactcggacg gacggttaca caaatttgct
    36721 cgccgcctat cattttgtct ttgggttgcg aaggtttatc gtttacaata accgcgcctt
    36781 ctttaatcca tgatgtcagg cggctgcgcg aatagtcggg cagaagtttc gccaacaccg
    36841 catccaaccg cccgccggca agctcgggcg gaacggtcag accgatacgg ttttcagttc
    36901 cggaagctga cgcaaagtct gaatcgtcgc tataatcgga ttcattatca aaggaagtat
    36961 tctgcatgaa aaaaattctt ttaacggttt cattaggttt ggcactgagt gcctgtgcca
    37021 ctcaaggtac ggccgacaaa gatgcccaga ttacccaaga ttggagtgtg gagaagctct
    37081 atgccgaagc ccaggacgaa ttgaacagca gcaattatac gcgggctgtc aagttatacg
    37141 aaatcttgga atcgcgcttc cccaccagcc gccatgcccg gcaatcccaa ctggataccg
    37201 catacgccta ttataaagac gatgaaaaag acaaggctct ggcggcaatc gaacgcttcc
    37261 gccgcctcca tccgcagcat ccgaacatgg attacgcgct gtacctgcgc ggcttggtgc
    37321 tgtttaacga agatcagtcc ttcctgaaca aactggcctc gcaagactgg tccgaccgcg
    37381 acccgaaagc caaccgcgaa gcgtaccagg cgtttgcgga actcgtccaa cgcttcccga
    37441 acagcaaata cgccgccgat gcaaccgcac gcatggtcaa actggtcgat gctttgggcg
    37501 gcaatgaaat gtcggtggcg cgttactaca tgaaacgcgg cgcatatatc gccgccgcca
    37561 accgcgccaa aaaaattatc ggcagctatc aaaatacacg ctatgtcgaa gaatcactgg
    37621 ccatattgga acttgcctac aagaagttgg acaagcccca gcttgccgcc gatacgcgcc
    37681 gcgtattgga aaccaacttc ccgaaaagcc cgtttttgac gcacgcttgg cagcccgacg
    37741 atatgccctg gtggcgttac tggcattaag cccatcctcg aaatgccgtc tgaagccctt
    37801 tcagacggca ttttccgttc cggcaccagc cttcccgttc actcaaacgg ttttttctgc
    37861 caatagaatt tatacactct caaacgttga gcctttacgc ctccgcgtcc caagccgaat
    37921 tgcagcgcgc ccgacaaatc cgtccgcaac aacttgatgc cgtgtgcgcg gacacggttc
    37981 tgcaccgctt cggtcggatg cttgtaggcg ttggcataac cgcttgaagc aacggcatat
    38041 tcgggcgaaa cggcattgag gaacacgccc gacgaggacg tattgctgcc gtgatgcccc
    38101 aacaccaaca cctggctgta caggttgcct ccatacttgc cgaccaggct ttcctcgccc
    38161 ttcgtatcca aatcgcccgt taccagcagt gccgcaccgc ccgccacaac acgcaaaaca
    38221 caacttttcc cattatcatc gatatttttg cgttcagacg gcctcaaaaa ctcgaaatcc
    38281 acgccgtccc attgccaacg ctgttccgca caatgccgcg ccccctcata aaattccggc
    38341 tgtccggcat aaatcccgcc gttcggtatc ttgccgactg cctgaaagcc gccgtcgtgg
    38401 tcgctgtcgt gatgcgacag aaccagcttg tccaaacggc ggacacccgc cgcattcaaa
    38461 ctcggcacga tacccgtttg tgccgccgct accgttcccg tatcgaaaag cagatggcgg
    38521 tttgccgtcc gcacaagcac cgacaagccc tgccccgcat cccaaaccgt aaccgcagct
    38581 tcgttttcag gtactgcttc aggacggtag gatacaaacc ctgccaacag caacaccgcc
    38641 cacggacgca aacccaagcc gcgcggcagc agcaacagca aagcggcaca caccgccaac
    38701 accagcagcg gcaaaggtgc ggcggcaacg gcaaactcag gcgacacatc ggcaagccac
    38761 accaaaaacc gcaaagtgta ttccgccaaa aatgcccccg cctgctgaag cggcgcaaac
    38821 ggcacgaccg aacccagcaa cgccagcggg gtcaacaccc aagaaaacca cggtatcgac
    38881 accgcattga ccaaagggct gaccaaaggc agcgaggcaa acagataacc gagcaaaacc
    38941 agagacaaca ccgaagccgc ccactgcccg cgcacggcgg tttgccgttt cccctcgtac
    39001 aaacgccctg cacacgccca tatcagggtc gccaccaaac cgaaagacag ccaagtaccg
    39061 acacccaaaa ccgccaaagg gtcgaacagc agcaccgccg ccaacgcctg ccaccacgtc
    39121 gcccacgccg acagtcttcc cctgcgccac gcccacgcga acgccgccaa catcaaaaca
    39181 ctgcgctgcg tcggcacgga aaaaccggca agcagcgcgt aaaacagcgc gcccgcacac
    39241 cccgccgcca aaacccacgc gcgcggccgg gcgggaaggc gcggggaaca ggcaagcagc
    39301 cgcttcgcca gccacgcaaa cagcaccgcc accatcgtaa cgtgcaaacc cgagatactg
    39361 accaaatgcg tcagccccaa cggccggaac gcctgccaca atccggggcg caatgccgac
    39421 tgttcgccca cgctcaacgc gcgcatcagc ccgatgccgt ctgaaagccc gccgtccgca
    39481 tccgcctgcc gccaattgcg gctgatgcgg ctgcgccaaa ccgcaatccc ccaaccgctt
    39541 ccgccatgca gcaaaaccct gtccgcaccg accgtcccca cgccgcctac cccgttggat
    39601 aatgcccacg cctcacggtt caaaccgcgc aggttcaact ccccgacgac ggggtgcacg
    39661 cgtgccgtta tccgccatct gctcccgacc gcccattcgc gccgtttgta gtccgacagc
    39721 agcaaatcaa acgtccgacc gccgctgtcc acagcctttg ccgcaaactg cacgcgccgc
    39781 ccgtccgacc tcggcatatc cgtcacttcc accgtcaaag gcacgcctga aaccgcctcc
    39841 gcccgccatt gcgaagacag tgccgcttcc gttctgaata cgccgtaagc cgcgcccgcc
    39901 aacacgcaca gcatcagacc ggcaaacgca aaacgccttg caagcacagc caaaaccgca
    39961 aaagccgcca accaaaacgg ccaatgcggt acaaccgaca gtgcgaacga aactgccacg
    40021 ccgacaaccc aacacggcaa aaaccaacga cggaacacgt ctccccactc ccgatataaa
    40081 aaacaaacga tgataaagca aagccataaa accgtcataa caagatttga aaatgataag
    40141 aatgcaaacg aaaagcctca gtattatccg agacaacggc aaactttcca tcgtcattcc
    40201 cgcaaaagcg ggaatccaaa gacaaaaagc cgcagaaatt tatcggaaag aaccgaacca
    40261 caaaaaccgg cttcccatct tcgcttgaat aacagaacaa agaaagcgga aataaaaaca
    40321 tcaggattct tttttaaatt ggcaataaaa caccaaaatg aatgagttac acaaaaaaat
    40381 actcaacacc acccaaccgg cgtaaaatgc aaaacattat cgctattaaa acggtaaaac
    40441 cttatgaaag ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
    40501 gcccttacgc catactcaga agcggcattg gtgagagacg atgtcgatta tcaaatattc
    40561 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
    40621 aacaaacaag gtcaaaacat cggcaacgcg ttatccaacg taccgatgat tgattttagc
    40681 gttgcagatg tcaacaaacg catagcaacg gtagtcgatc cccaatatgc cgtcagcgtc
    40741 aaacacgcca aagcagaagt ccatactttt tattacggcc aatacaacgg tcataatgat
    40801 gtagccgaca aagaaaatga ataccgcgtt gtcgaacaaa ataactatga accccataaa
    40861 gcttggagtg cgagcaactt aggccgcctc gaagattata atatggcgcg ttttaataag
    40921 tttgtaaccg aagttgcgcc gattgcacca actgatgccg gcggcggatt gaatacctac
    40981 aaagataaaa accgtttttc ctcttttgtc agaatcggtg cgggcaggca attagtttat
    41041 gaaaaagggg tttatcatca agaaggaaat gaaaaaggct acgatttgcg cgatctttca
    41101 caagcctatc gttatgccat tgcaggtacg ccttataaag atattaatat tgaccaaaca
    41161 atgaataccg aaggtttgat cggctttggt aatcataata aacagtattc ggcagaagaa
    41221 cttaaacaag cactttcgca agatgcgtta acaaattacg gcgtgttggg cgatagcggc
    41281 tctccgttat ttgcttttga caaacaaaaa aatcaatggg tctttttggg aacttacgat
    41341 tattgggcag gttacggaaa aaaatcatgg caagaatgga atatctataa aaaagaattt
    41401 gcagataaaa tcaaacaaca cgataacgcc ggcaccgtca aaggtaatgg agaacatcat
    41461 tggaaaacca cgggtacaaa cagccatatc ggttcgacag cggtaaggct tgccaacaat
    41521 gaaggagatg cgaacaacgg acaaaatgtt acctttgaag acaacggcac tttggtattg
    41581 aatcaaaaca tcaaccaagg cgcgggcggc ctgtttttca aaggcgatta cacagtcaaa
    41641 ggtgcaaata atgacatcac ttggttaggt gcggggattg atgttgccga cggcaaaaaa
    41701 gtcgtttggc aagtcaaaaa tccgaatggc gacagattgg caaaaatcgg caaaggcact
    41761 ttggaaataa acggcacagg cgttaaccaa gggcaattaa aggtcggcga cggtacggtt
    41821 attctgaatc aaaaagccga tgccgacaaa aaagttcagg ctttctccca agtcggcatt
    41881 gtcagcggac gcggtacatt ggtattaaat agttcaaatc agattaatcc cgataaccta
    41941 tatttcggtt tccgtggcgg tcgtttggat gccaatggca atgacttgac ttttgaacac
    42001 atccgcaacg tggatgaagg cgcgcgcatt gtcaaccaca acacaggcca cgcctccaca
    42061 atcacactaa cgggtaaatc tttaattacc aatccgaaca gcctttcggt acatagcata
    42121 caaaatgact atgatgaaga tgattatagt tattattacc gaccaaggcg acccatccca
    42181 caaggcaaag atctttatta caaaaactat cgttattacg ccctaaaatc cggcggcaac
    42241 gtgaacgcac cgatgcccga gaacggtgta gcagaaaata acgactgggt ctttatggga
    42301 tatacgcaag aagaagccaa gaaaaacgcg atgaaccaca aaaacaatca gcgtattagc
    42361 ggttttagcg gtttctttgg cgaagaaaac gaaaaaggac ataacggggc attaaacctt
    42421 aatttcaatg gcaaaagcgc gcaaaaccgt ttcttgttaa caggcggcgc gaatttaaac
    42481 ggaaaaataa gcgtaactca aggcaatgtc ttgttatcag gtcgtccaac accacacgca
    42541 agagattttg tgaacaaatc ttcagcccga aaagacgcac atttctccaa aaacaatgaa
    42601 gtcgtatttg aagacgactg gataaaccgc acattcaaag ccgcagaaat tgcggttaac
    42661 caatccgcat cattctcttc cggaagaaat gtatccgaca tcaccgccaa catcaccgca
    42721 acagacaacg ccaaagtaaa tttgggttac aaaaacggcg atgaggtttg cgtgcgctcg
    42781 gactataccg gttacgttac ctgcaacaca ggcaacttat ccgataaggc tttaaacagc
    42841 tttgatgcga cacggattaa cggcaatgtg aatttgaatc agaatgcggc attggtcttg
    42901 ggcaaggctg cattatgggg gaaaattcaa gggcaaggaa acagccgtgt cagcctaaac
    42961 caacatagca aatggcattt gaccggcgac agccaagtac acaatctgtc attggcggat
    43021 agccatattc atttgaacaa cgcttccgat gcgcaaagtg caaataaata ccacacgatc
    43081 aaaatcaatc atttatccgg taacgggcat tttcattatc tgacggactt ggcgaaaaat
    43141 cttggggata aagtgcttgt gaaggaatcc gcatccggcc attatcagct ccatgttcaa
    43201 aataaaacag gcgaacctaa tcaggaaggg ctggatctct ttgatgcatc atccgtacaa
    43261 gaccgctccc gcctttttgt ttccttggca aatcattacg tcgatttagg cgcattgcgt
    43321 tatacaatca aaacagaaaa cggtattacc cggttgtaca atccttatgc cgggaaccgc
    43381 cgcccagtca agccggcccc gtctcctgcc gcaaacacgg cttctcaagc acaaaaggca
    43441 acacaaacgg acggtgcaca aattgccaag cctcaaaata tcgtcgtcgc accgcctagc
    43501 ccgcaggcaa atcaagccga agaagccctc cgccaacaag caaaagcgga gcaagtgaag
    43561 cgtcagcaag cagcagaagc agaaaaagtt gcacgtcaaa aagacgaaga ggcaaaacgc
    43621 aaagcagccg aaattgctcg tcagcaggaa gaagcacgaa aagctgcaga gttagccgcc
    43681 aaacaaaagg cggaagcaga gcgtaaagcc agagagttgg caagacaaaa agcagaagag
    43741 gcaagtcatc aagctaatgc caaaccaaaa cgtcgtagac gtcgggctat attacctaga
    43801 cctcctgcgc cagtattttc attggatgat tatgatgcaa aagacaatag tgaatcatca
    43861 ataggtaatt tagctcgtgt aatacctaga atgggaaggg agttaattaa tgattatgaa
    43921 gaaatcccct tggaggagtt ggaagatgaa gcggaagaag aacgtcgcca agcaacgcaa
    43981 ttccaaccca aaagtcgtaa ccgtagagct atatcatcgg aaccatcatc tgatgaagat
    44041 gcatctgaat cggtttccac atcagacaaa caccctcaag ataatacgga acttcatgaa
    44101 aaagttgaga cggcgggttt acaaccaaga gccgcgcagc cgcgaaccca agccgccgcg
    44161 caagccgatg cagtcagcac caatactaac tcggctttat ctgacgcaat ggcaagcacg
    44221 caatctatct tgttggatac aggtgcttca ttaacacggc acattgcaca aaaatcacgc
    44281 gctgatgccg aaaaaaacag tgtttggatg tcaaacaccg gttatggccg tgattatgct
    44341 tccgcacaat atcgccggtt tagttcgaaa cgcacgcaaa cacaaatcgg cattgaccgc
    44401 agcttgtccg aaaatatgca gataggcgga gtattgactt actctgacag tcagcatact
    44461 tttgatcagg cgggcggcaa aaatactttt gtgcaagcca acctttatgg taagtattat
    44521 ttaaatgatg cttggtatgt ggccggcgat attggtgcgg gcagcttgag aagccggtta
    44581 caaacgcagc aaaaagcaaa ctttaaccga acaagcatcc aaaccggcct tactttgggc
    44641 aatacgctga aaatcaatca attcgagatt gtccccagtg cgggtatccg ttacagccgc
    44701 ctgtcatctg cagattacaa gttgggtgac gacagtgtta aagtaagttc tatggcagtg
    44761 aaaacactaa cggccggact ggattttgct tatcggttta aagtcggcaa ccttaccgta
    44821 aaacccttgt tatctgcagc ttactttgcc aattatggca aaggcggcgt gaatgtgggc
    44881 ggtaaatcct tcgcctataa agcagataat caacagcaat attcagcagg cgccgcgtta
    44941 ctgtaccgta atgttacatt aaacgtaaat ggcagtatta caaaaggaaa acaattggaa
    45001 aaacaaaaat ccggacaaat taaaatacag attcgtttct aaaatactaa attcatagca
    45061 aaataaaatg ccgtctgaac tcaagcttcg gacggcattt ttatcagact aacaaagcta
    45121 cagctcaatg cctttgagtt tcgccacggt attgatgtct ttgtcgccgc gacccgacag
    45181 gttgaccaaa atcacttggt ctttacccat tttcggcgcg tttttcaccg cccaagcaac
    45241 ggcgtggctg gattcgagcg cggggatgat gccctcgaat cggcagagca agtcaaaggc
    45301 ttcgagtgct tcgtcatctt tggcaacggt gtattcgacg cgcttgatgt cgtgcagatg
    45361 gctgtgttcc gggccgatgc cggggtaatc caagcctgcg gaaacagagt gcgtccccaa
    45421 gacttggccg ttttcgtcct gcatcagata gctgcggaag ccgtgcagta cgccaatcgg
    45481 tgcgcccgaa gtaatcggcg cggcgtggtc aggcgtatcc acgcccaaac cgcctgcttc
    45541 tacgccgacc aaacgtacgt tttcttcttc gatataggga tagaacaaac cgatggcgtt
    45601 cgagccgccg ccgacgcagg caacggcgac gtcgggctgt ctgccggtgg cttcctgcat
    45661 ctgcgctttg gcttcgttgc cgatgacgca ttggaaatca cgcaccattt cgggatacgg
    45721 cgcggggccg gcggcggtgc cgatgatgta gaacgtatcg tccactcggg cgacccattc
    45781 gcgcatcgct tcgttcatcg cgtctttcaa agtacggctg ccactgtcaa cgccgatgac
    45841 attcgcacca agcagcttca tacggaacac attcggcatt tggcgttgaa tatcgtccgc
    45901 acccatgtac acgtcgcaag tcatgccgaa acgtgcggcg acggtggcag aagccacgcc
    45961 gtgctgaccc gcgccggttt cggcgatgac gcgttttttg cccatacggc gggcaagcaa
    46021 tgcctgaccg atggtgttgt tgaccttgtg cgcgccggtg tggttcaagt cttcgcgctt
    46081 caaccagatt tgcgcgccgc ccaaatgctc ggacaaccgc gcggcgtggt aaacggggct
    46141 gggcctgccg acataatgtt tcaaatcgcg gcggaactcc gcccaaaatt caggatcgtt
    46201 tttcgcttcg ttgtaggcct gttccagctc tttcaaagcg ggaatcaggg tttcggaaac
    46261 ataaagcccg ccgtgttcgc cgaaaaagcc cttctcgtcg ggcgcgtggt agttcttcat
    46321 cggattcttc cttatgcttc aggttttcag acggcatttc cgttttttca aacatgccgt
    46381 ctgaacggga aattgtcggc aattataact gcttttcaca caagcccgcc atttcgaaaa
    46441 acgcccgata cgccgctgcc tttttcgcca aactcaaagg ataggcgcgc gaagtcgaag
    46501 gcaggcgcgt cagtgtcaaa tcccgtccgg caaacggaaa cggcacggtt tcgcccgttt
    46561 tcggcatttt gatgccgccg ccctgaatat cgagcaggat ttccgtcgcc ttgccgcctg
    46621 tcgtacaaat atggcggcag tctggtattt tcgccaacac cgccgccaaa tcgacggttt
    46681 caactacctt taaaaacttg tcggacgcat tgccgtgctc ccttgccgcc ttcaacacgg
    46741 tcggacagga cgcaatcccc cgttcgtgca aaaacgcctt gattttctcc gcgtcaaacg
    46801 ctttttcaga gtgcgcagcc tccccgtggg acggcatccg gaaatgcgcc gcatcgttaa
    46861 aaaacaccag cccgtaaacg cgccacatat cgttttggaa attcggataa tgaaactgca
    46921 tcgcgcgttt gtcttccttg ggcggaaacg tccccatcat catgaccgca gccttcggcg
    46981 gcaacacggg atcgaaggga tgggtttcga tttcaagcag attttcagac acgttcaatc
    47041 ctccaacagg caaagcggca gacagccggt ttgcaccaaa ccgccaaaca cggcaagcct
    47101 tcaaacagca ttatcacgcc ctgccgtttt cggttacaat gccgacatca aacggatact
    47161 gtaaacacat ttatgttcca acacacagga cgacacataa agcaccgccc tatgtgtcgt
    47221 cctgatttgg aaggggttac gccccgccca aacagagtct gatcctgccg ccctaaaggg
    47281 cggggtttca accgaagagg aaacacgatg aaagaacaca aagcccgcaa gcgtttcggg
    47341 cagaattttt tgcaggacac gcggattatc ggcgatattg tcaacgccgt gcgcccgcag
    47401 gcggatgatg tcgtgattga aatcggcccg ggtttggcgg caattaccga acctttggcg
    47461 aaaaagctga accgcctgca cgttgtcgaa atcgaccgcg acatcgtatg ccgtctgaaa
    47521 acgctgccgt ttgcggataa attggtgatt cacgaaggcg atgtattgca gtttgatttc
    47581 aacaacattg aaggcaaaaa gaaaatcgtc ggcaacctgc cctacaacat ttccacgccg
    47641 cttttgttca agctggcgga agtggcggac gatgtcgccg atatgcactt tatgctgcaa
    47701 aaagaagtgg tcgaccgtat ggttgccgcg ccgaaaagca atgactacgg gcgtttgggc
    47761 gtgatgctgc aatatttctt cgatatggaa atgctgattg acgtgccgcc cgaatcgttc
    47821 gatcccgcgc cgaaaatcga ttccgcagtg gtgcgcatga ttccggtgaa acaccgtatc
    47881 ggcaaggcgg acgatttcga gcatttcgcc aaactggtga aactcgcctt ccgccaacgc
    47941 cgcaaaacca tacgcaacaa tctgaaagag cttgccgacg acgatgattt gcaggcagtc
    48001 ggcatcagtc cgcaagaccg tgccgaacac atcgcgccgg agaaatatgt ggcgttgagc
    48061 aattatctgg cggacaaggc cgtctgaaaa gggacgcggc gatggaaagc aacctgcaga
    48121 aaaccgagcg tttgatccgc gaaatcaacc gcctgcacgc acaatattcg caggattatt
    48181 ttgaaaccgg caaagtgcgg aaaatcaatt tgagccacac gctcaaaaac gttccgaccg
    48241 aacatatttt gtcttaccgc cttaatttgc acgaagcggt taacgattat ttagcctttg
    48301 ccgatacgcg cggcatagac tttttctacc gcgtgaaaac cgcagaaagc atacatgata
    48361 aaatcaaccg ctatctggca cgcggcaccc aatatccgac caacaacatc ctgaacgata
    48421 ttttcggcgc gcggctgatt tggccgtctg aaacagtcgc cgggatttta gaaaaacttg
    48481 acggctggaa aacagaatac ggcttgaaaa actggtattt gcgcgatgcc ggcggctata
    48541 tcggcataca catttatttc aaaaacagca gcaatttcta ctatccgtgg gaattgcagg
    48601 tttgggatga aaaagatgcc aaagccaata ttgaaaacca tatggcgtac aaacggaatt
    48661 tcgtgcgttg aattgccggc aagccgcctt gaaaccgttt ataactgttt ataagagaat
    48721 aaaccataaa atacacaaac gtattcctga cttttccttt aaccgcccga gtgaacaaac
    48781 tatgaaatac acagccgcac tcctgacttt tcttttaact gcctgcatga acccgaacga
    48841 tgcgtttttc caaaaccggc gttatcaaat gccggaggcg caattgaacg gttcaaacgc
    48901 cgttttccat tacggttatt cccaaaatcc cgatcatgat ttattggtcg accaagtccg
    48961 cgtgccggac gcgtacaggg cttcatttgc gcgggattgg atagcgaagg agcaaggtta
    49021 ccgcaaaaac gactgcgtac tggaaaaagc ggaaagcggg ggtgtgacgt actacacctg
    49081 cgatgcggaa aaaggattca acggcacacg tttcgtcatc tatgtcgtga atatgaaaaa
    49141 cgacaaaggc tacgtcaaag tttaccgggg catgaataag ccgaatcagg aagagttaac
    49201 caaattggtt gccgatttga ataagtttta cccttaatca gccagacgga ttcagactgc
    49261 ccgaaaagaa tggacactat gatcaaattc aaaaacgtac acaaacattt caaagacctg
    49321 cacgtcatca acggcgtaaa cttggaaatc aaaaaaggcg aagtggtcgt cgtctgcggg
    49381 ccttcgggca gcggcaaatc cacactcatc cgcaccgtca accagcttga aagcatcgaa
    49441 agcggcgaga tttgggtgga cggcgtcaac gttgccgacc ccaaaaccga tttgaacaaa
    49501 atccgcgaag aagtcggctt tgtgtttcaa agtttcaacc tctacccgca cctgaccgtg
    49561 ttggacaaca tcaccctcgc accgatgaag gttaaggggc aaaatgcgga acaggcggag
    49621 aaaaaggcaa tggagctttt ggaacgcgtc ggactggcac acaaaaaaga cgccttccct
    49681 tcccaacttt ccggcggcca gcagcaacgc gtggcgattg cgcgcgggct ggcgatggag
    49741 ccgcgcgtga tgctgtttga cgagccgact tccgcactcg accccgaaat ggtcggcgaa
    49801 gtgttgaaag tgatgaaaga cttggcggaa agcggcatga cgatgatgtg cgtaacccac
    49861 gaaatggggt ttgcccgcga agttgccgac cgcgtgattt tcgtcgataa agggcaaatc
    49921 cttgaagacg aaacgccgga agcgtttttc accaacccga aacacgaacg cgccaagcag
    49981 ttcctgcaac aagtcatgac ccattgatgc tatgccgtct gaaaaccgaa cgttcagacg
    50041 gcatagcccg acaccgcaag atgattgaga aagcacacca tgtattttgt tgaccgcacc
    50101 gccgccgtcc tcaagccgac cgcgcgcttt ttggaatggc tcaaaagcac cgacgaaaat
    50161 atgcccgacc tgaccataga acagcttcgc gccgactgtt ccgtcttcct cgttccgcag
    50221 ttcgacgaac cggaagccgt cgtttcctac ttcgacgaac gctaccggca gatttttgag
    50281 gcggagctgg caggttggga catcgacaag gacaaatggc cgcaagatat ggggctgaag
    50341 gcgttttggg aatttttcga cattgaaatc cacgatatgg ttttggatat ggaagaagcg
    50401 gatttgaaca tcacgcccgt gttcgacaat atgatgtaac gccatgcgcg cctttcagac
    50461 ggcattgagg ccgtcccgca tcctgaacat cctgaccgta tcactccact cggcttccat
    50521 tgccgtctgc ctgacttggt tttacggccg gatgatgtgg ttcggtttgg cggctttggc
    50581 ggcaagttac gcatactcgc tcaggataac gaatctgaaa caccgccatg ccataaccgc
    50641 cattaccata gaccgcgacg gacgggcgga aatcgtatcc ggcaaagaca aaacagcggc
    50701 aacacttgcg ggcagcagta tggttacgcc ttatgcctta ttcttacaat gggacacagg
    50761 cggcaaaacc gtccgccaat gcatcatgcc cgacatgacg gacaaagaat cctaccggcg
    50821 gctgaaagtc tgggtgcttt ggcggcagcc gaaaaaaacc gccgaaaccg atacatcaga
    50881 ctgatttcag acggcatttg aaaacgaaac actcatgaaa acactacaag actggctctc
    50941 gcatttggaa accgcccaca gcggcggttt gatcgacatg ggtttggagc gcgtaagcga
    51001 agtaaaaaag cgcatgaacc tgacaccgca atgccccgtc gtcgtcgtcg cgggaacaaa
    51061 cggcaaaggt tcggtctgcg cctacctgac acagatttac aaacaggcag gcttcaaaac
    51121 cggcacgctg accagcccgc atttattgca ttacaacgaa cgcatcgcca tcaacgccga
    51181 acccgtttcg gacgatacga ttattgcctc tttcgagcgc atcgaagccg cgcgcggcga
    51241 aatatcgctg acttattttg aattcaatgc cttggcggct gtcgacatct tcatacgcga
    51301 acaagtggat gtaatgatat tggaagtcgg cttgggcgga cgtttggacg cggtcaacgc
    51361 gtttgacggc gattgcgcgg tggttactag tgtggatttg gaccatcagg catttttggg
    51421 cgatacggtc gaacaggtcg gctttgaaaa ggcaggcgtg ttccgcagcg gcaaacccgc
    51481 catctgcggt caaaaccccg cgcccgcatc attggtcgca cacgccgaag ccataggcgc
    51541 gaaactgctg atggtacagc gcgatttcga gtttcacgcg atggaaaaca tccaatggaa
    51601 ctaccgcttc cggccgcagc attcagacgg tcccgcacgc aaccgcaatg ccctgccctt
    51661 ccccgcattg cgcggcgcat accagctttc taacgccgcc tgcgcgttga ccgtattgga
    51721 atgcctcgac gacagactgc cggtggacat tggtgcaatc aaacgcgggc tgctgctggt
    51781 tgaaaatccc ggacgcttcc aagtcctgcc cggccggccg ctgaccgtat tggatgtcgg
    51841 acacaacccc cacgccgccc gcgccctgcg ccgcaacctg atcaaccttg cctacgcgca
    51901 aaagcgcacc gccgtgttca gtatgctgtc cgacaaagac atagacggcg tgtcggaaac
    51961 cgttaaagac cagtttgacg agtggtatat cgcgcctttg gacgtgccgc gcggtatgac
    52021 ggcggatgca ctgaaagcca aattggaaca acaccacatc gaaaacatac agacattcgc
    52081 cgccgtccgc gacgcgtacc gggcggcagc ctcaaaagcg ggcgaagacg acagaatcgt
    52141 cgtattcggc tcatttcata ccgtcgccga cgtaatgtcg gtactgtgaa aaggaaactt
    52201 ctatgtccga aaacaaacaa aacgaagtcc tgaccggtta cgaacagctg aaacggcgca
    52261 accgccgccg cctcgtaacg gcaagctccc tggttgccgc ctcctgcatc ctgctggcag
    52321 ccgcactcag ttccgatcct gccgacagca atcccgcacc gcaggccggc gaaaccggcg
    52381 caacggaaag ccaaacggca aacacggcac aaacccctgc cttgaaatcc gccgccgaaa
    52441 acggggaaac cgccgccgac aaaccgcagg acttggcagg cgaagacaag ccttctgccg
    52501 ccgacagcga aatcagcgag cctgaaaacg taggcgcgcc gctggtgctg attaacgacc
    52561 ggctcgaaga cagcaacatc aaaggtttgg aagaatccga gaaactgcaa caggcagaaa
    52621 ccgccaaaac cgaaccgaag caggcaaaac aacgcgctgc cgaaaaagtg tcggcaactg
    52681 ccgacagtac ggatacggta gcggttgaaa aaccgaaacg ctctgccgaa cccaaaccgc
    52741 aaaaagcgga acgcactgcc gaagccaagc ccaaagccaa agaaaccaaa accgccgaaa
    52801 aagttgccga caaaccgaaa actgctgccg aaaaaaccaa accggatacg gcaaaatccg
    52861 acagcgcggt aaaagaagcg aaaaaagccg acaaggctga aggcaaaaag acagccgaaa
    52921 aagaccgttc ggacggcaaa aaacacgaaa cggcgcaaaa aaccgacaaa gcggacaaaa
    52981 ccaaaaccgc cgagaaggaa aaatccggca aggcgggcaa aaaagccgcc attcaggcag
    53041 gttatgccga aaaagaacgc gccttgagcc tccagcgcaa aatgaaggcg gcgggtatcg
    53101 attcgaccat caccgaaatc atgaccgaca acggcaaagt ttaccgcgtc aaatcaagca
    53161 actataaaaa cgcaagggat gccgaacgcg atttgaacaa actgcgcgtg cacggcatcg
    53221 ccggccaggt aacgaatgaa tagcctcccc attgccgacc tcctcgcctc cgccgtcatc
    53281 gccgcctgca tcgtcatttc cacgatgcgc ggcgtgattg cggaagcagg ttcgatggtg
    53341 gcatgggtgg tttccttctt ttttgccaaa ctctttgccg cacccttcgc cgacctcgcc
    53401 tttgcctcgt tccaaccccg cctgtttgca ttggctctgt cattcatttc cctgttcgtc
    53461 attgcctgtc tgatccagaa aatgctccgt tcgctgctga ccggcgcagt ttcggcggtc
    53521 ggtctgggct ttgccaaccg cattttgggc ggtgtattcg gtgcattgaa aggcgttttg
    53581 attgttaccc tgctgatcat gcttgcttca aaaaccgacc tgcccgatac cgaagaatgg
    53641 caacagtcct ataccgtacc gtttttcgta tcgctttccg aagcggtgtt aaaccatacg
    53701 gacaacgcac ccgaatccct cgacgacgac taatccgcat acgtggcgtt tcagacagca
    53761 tacctgattt atgccgtccg aaacgccttt tctttccttg acacaccccg acagacaaag
    53821 cgcacctgcc tgactttaca caaatattgt caacaattcg ggcaaaaagc cgccgtttgc
    53881 tttataattc cgtttcaccc ttaaacctcg aatcggagaa gacgatatgt gcggcgtatt
    53941 aggtttggtc agtcatgaac ccgtgaacca gcttctgtac gacggtttgc agatgttgca
    54001 gcacaggggg caggatgcgg cgggcattgc aacggcggaa ggcggtacct tccatatgca
    54061 caaaggcaaa gggatggtga gcgaagtgtt ccgcacgcgc aatatgcgcg atttgaccgg
    54121 caacgccggc atcgcccacg tccgttatcc cacggcgggc aacgccggca gcagcgcgga
    54181 ggcgcagcct ttctacgtca gctcgccttt cggcatcgtt ttggcgcaca acggcaacct
    54241 caccaacact gccgaactgt atgaaaacgt gtgtaacaaa cacctgcgcc acatcaacac
    54301 cggctccgat tccgaagttt tgctcaacgt attcgcgcac gaattgcgcc gcgaagtctc
    54361 taaaaacgcc gacccgcacc ggctcaatgc cgacaatatt ttcaacgccg ttgccgaagt
    54421 ccaccgcctg gtgcgcggtg catacggcgt ggttgccatg attgcgggct acggtatgct
    54481 cgccttccgc gacccttacg gcatccgccc gctggcattg ggttcgcaaa ccgacagcga
    54541 aggcaggaaa tcctatgccg tcgcctccga atccgtcgcc ttcaatgcgc ttgcctacga
    54601 tttggaacgc gatatccggc cgggcgaagc ggtatttgtc ggctttaacg gcacaatcat
    54661 cgcccgacaa tgcagcgacc gcgccaaact cagcccctgc ctttttgaat atgtttattt
    54721 tgcccgcccc gactccgtga ttgacggcgt gtccgtttac cagtcgcgct tggatatggg
    54781 cgtgtccttg gcggaaaaaa tcaaacgcga gctgcccgtg gacggcatcg acgtcgtgat
    54841 gcccattccc gacaccagcc gccccagtgc aatggaactt gccgtccacc tcaaaaagcc
    54901 ttaccgcgag ggtttgatta aaaaccgcta tatcggccgc acctttatca tgcccggtca
    54961 gtcgacgcgc aaaaaatccg tgcgccaaaa actcagcccg atggaaaccg aatttgcagg
    55021 caaaagcgtg ttgctggtgg acgactccat cgtgcgcggg acgaccagcc gcgaaatcgt
    55081 cgaaatggta cgcgcggcgg gcgcgcgcaa agtctatatc gcctccgccg cgcccgaagt
    55141 gcgctatccc aatgtgtacg gcatcgatat gcccacgcgc gaagagttga ttgccaacgg
    55201 gcgcagcgcg gcggaaatcg ccgccgaaat cggcgcggac ggcatcgtgt ttcaagactt
    55261 gggcgatttg gaagccgtcg tcaaagcact caacccgaaa atcgaatcct tcgattcgtc
    55321 ctgtttcaac ggcatttatc gaaccggcga catcgatgat gcctacctcg accgcctgtc
    55381 tgccgagaaa tccggctgcg ccggtctgaa aatccacccg agcaggatgg aacacagcat
    55441 cagcatcagc gatgcgggcg acgaagaata aggcttccca aaccgcgatg ccgtctgaaa
    55501 tccgaacgtt gggttttcag acggcatttc ccatatcggc atcacatttc ccgtttcatc
    55561 cgcacacaat atgagtaccg aaaccaaaaa ctacattacg cccgccggat ggcaggcatt
    55621 gaaagacgag ctttatcaac tggtcaataa agaacgcccc gaaatcgtcc aaatcgtcaa
    55681 ctgggcggca ggcaacggcg accgcagcga aaacggcgac tatctttacg gcaaacgccg
    55741 tatgcgcgaa atcgaccgcc gcatccgttt cctgaccaaa cgtttggagg ctgccgtcgt
    55801 cgtcgatccc gaattgcgcg aagcgaccga ccaagtattt ttcggcgcga ccgtcggtct
    55861 gttgcgcggc gacgggcgcg agcaaaccgt caaaatcgtc ggcgtcgatg aaatcgatac
    55921 cgcgcaaaac aaaatttctt ggatttcgcc gttggcgcgt tgcctgatta aggcgcgcga
    55981 aggcgacgaa gtggttttga acacgccgga aggccgtgag gaaattgaaa tcctttctgt
    56041 cgaatacatc aaaatcgact gatcgggaat gccgtctgaa acggcacaag cgcaccgttt
    56101 cccaaataaa ggaataccga atgaggaaaa tccgcaccaa aatctgcggc atcaccacac
    56161 cggaagacgc actgtatgcc gcccacgccg gcgcagacgc attgggactg gttttttacc
    56221 cccaaagccc ccgcgctatc gacatcatta aagcacaaaa aatcgccgcc gcactgccgc
    56281 cgtttgtcag cgttgtcgcc cttttcgtca acgaaagcgc gcaaaacatc cgccgcatcc
    56341 ttgccgaagt gccgatacac atcatccaat tccacggcga cgaagacgat gcattctgcc
    56401 ggcagttcga ccgcccctat attaaagcca ttcgtgttca gacggcatca gacatccgaa
    56461 acgccgccac gcgcttcccc aacgctcagg cactgctgtt cgatgcctat cacccttcgg
    56521 aatacggcgg caccggacac cgcttcgact ggacgctgtt ggcggaatat tcgggcaagc
    56581 cgtgggtgct tgccggcggg ctgacccctg aaaacgtcgg cgaagccgtc cgcatcaccg
    56641 gagcggaagc ggtcgacgta tccggcggcg tggaagcgtc taaaggcaaa aaagaccccg
    56701 ccaaagtcgc cgcctttatc gcaaccgcca accgcctatc ccgttaaagc aacaaaaatt
    56761 gccgccggaa tgacttatag tggattaaca
//
LOCUS       DAXDZG010000004        41750 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXDZG010000004 DAXDZG010000000
VERSION     DAXDZG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41750
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.004"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            196..1539
                     /locus_tag="ACFB78_000194"
     CDS             196..1539
                     /locus_tag="ACFB78_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC1268288.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEKVRRNLIQSGLWTALRTRPFSKIPAIDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1542..2774
                     /locus_tag="ACFB78_000195"
     CDS             1542..2774
                     /locus_tag="ACFB78_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC1268289.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2767..3543
                     /locus_tag="ACFB78_000196"
     CDS             2767..3543
                     /locus_tag="ACFB78_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC1268290.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3543..4169
                     /locus_tag="ACFB78_000197"
     CDS             3543..4169
                     /locus_tag="ACFB78_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC1268291.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4173..4766
                     /gene="nqrE"
                     /locus_tag="ACFB78_000198"
     CDS             4173..4766
                     /gene="nqrE"
                     /locus_tag="ACFB78_000198"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC1268292.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4780..5997
                     /gene="nqrF"
                     /locus_tag="ACFB78_000199"
     CDS             4780..5997
                     /gene="nqrF"
                     /locus_tag="ACFB78_000199"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC1268293.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6513..7568
                     /locus_tag="ACFB78_000200"
     CDS             6513..7568
                     /locus_tag="ACFB78_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC1268294.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7594..7809
                     /gene="nqrM"
                     /locus_tag="ACFB78_000201"
     CDS             7594..7809
                     /gene="nqrM"
                     /locus_tag="ACFB78_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC1268295.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="ACFB78_000202"
     CDS             complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="ACFB78_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC1268296.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIREINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8694..9512
                     /gene="cysE"
                     /locus_tag="ACFB78_000203"
     CDS             8694..9512
                     /gene="cysE"
                     /locus_tag="ACFB78_000203"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC1268297.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="ACFB78_000204"
     CDS             complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="ACFB78_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC1268298.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11262..11719)
                     /locus_tag="ACFB78_000205"
                     /pseudo
     CDS             complement(11262..11719)
                     /locus_tag="ACFB78_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11716..11862)
                     /locus_tag="ACFB78_000206"
     CDS             complement(11716..11862)
                     /locus_tag="ACFB78_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268299.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="ACFB78_000207"
     CDS             complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="ACFB78_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC1268300.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12350..13036)
                     /locus_tag="ACFB78_000208"
     CDS             complement(12350..13036)
                     /locus_tag="ACFB78_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1268301.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13223..13495
                     /locus_tag="ACFB78_000209"
     CDS             13223..13495
                     /locus_tag="ACFB78_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268302.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13703..15631
                     /gene="dnaK"
                     /locus_tag="ACFB78_000210"
     CDS             13703..15631
                     /gene="dnaK"
                     /locus_tag="ACFB78_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC1268303.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            15854..17774
                     /locus_tag="ACFB78_000211"
                     /pseudo
     CDS             15854..17774
                     /locus_tag="ACFB78_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            17831..18808
                     /locus_tag="ACFB78_000212"
     CDS             17831..18808
                     /locus_tag="ACFB78_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC1268304.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLAAGKEQFTVYGAPAMPSVT
                     IAVAALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTKGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            19145..19282
                     /locus_tag="ACFB78_000213"
     CDS             19145..19282
                     /locus_tag="ACFB78_000213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268305.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            19361..20130
                     /locus_tag="ACFB78_000214"
                     /pseudo
     CDS             19361..20130
                     /locus_tag="ACFB78_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20157..20849
                     /locus_tag="ACFB78_000215"
     CDS             20157..20849
                     /locus_tag="ACFB78_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1268306.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            21041..21655
                     /locus_tag="ACFB78_000216"
     CDS             21041..21655
                     /locus_tag="ACFB78_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC1268307.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            21951..23046
                     /locus_tag="ACFB78_000217"
                     /pseudo
     CDS             21951..23046
                     /locus_tag="ACFB78_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(23093..25282)
                     /locus_tag="ACFB78_000218"
     CDS             complement(23093..25282)
                     /locus_tag="ACFB78_000218"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC1268308.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPIGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTDQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEESLRALLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            25422..26222
                     /locus_tag="ACFB78_000219"
     CDS             25422..26222
                     /locus_tag="ACFB78_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC1268309.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKTESAVKAES
                     AGKSVAASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVG
                     ELINIDTRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRL
                     EHEFEKMTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSI
                     CDNPVAETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQ"
     gene            complement(26324..28258)
                     /locus_tag="ACFB78_000220"
     CDS             complement(26324..28258)
                     /locus_tag="ACFB78_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC1268310.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(28324..29502)
                     /locus_tag="ACFB78_000221"
     CDS             complement(28324..29502)
                     /locus_tag="ACFB78_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC1268311.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAVEREIRTGMKDSMNTEVKSGLKEGDKVVISEITAAEQQESGERVMGGPPRR
                     "
     gene            29728..30117
                     /locus_tag="ACFB78_000222"
     CDS             29728..30117
                     /locus_tag="ACFB78_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC1268312.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFEKYDSTKLKQNKYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            30391..31437
                     /gene="adhP"
                     /locus_tag="ACFB78_000223"
     CDS             30391..31437
                     /gene="adhP"
                     /locus_tag="ACFB78_000223"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC1268313.1"
                     /translation="MKMQAVVVNKNVAGDVEVVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGTHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAVGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(31738..35223)
                     /gene="smc"
                     /locus_tag="ACFB78_000224"
     CDS             complement(31738..35223)
                     /gene="smc"
                     /locus_tag="ACFB78_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC1268314.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            35596..36264
                     /locus_tag="ACFB78_000225"
     CDS             35596..36264
                     /locus_tag="ACFB78_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268315.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALRGSSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAVALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            36245..36433
                     /locus_tag="ACFB78_000226"
                     /pseudo
     CDS             36245..36433
                     /locus_tag="ACFB78_000226"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            36483..36716
                     /locus_tag="ACFB78_000227"
     CDS             36483..36716
                     /locus_tag="ACFB78_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268316.1"
                     /translation="MPDRAFGAGVSWKFGGKPTPAVAAQNAAHPAKVLQLRQEVAALR
                     ARQAETDRKLHKQAEMENELQQLRRALSELKKH"
     gene            36925..37647
                     /gene="lpxH"
                     /locus_tag="ACFB78_000228"
     CDS             36925..37647
                     /gene="lpxH"
                     /locus_tag="ACFB78_000228"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC1268317.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            37916..39502
                     /locus_tag="ACFB78_000229"
     CDS             37916..39502
                     /locus_tag="ACFB78_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC1268318.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAVGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(39668..39871)
                     /locus_tag="ACFB78_000230"
     CDS             complement(39668..39871)
                     /locus_tag="ACFB78_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC1268319.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            39987..40484
                     /locus_tag="ACFB78_000231"
     CDS             39987..40484
                     /locus_tag="ACFB78_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC1268320.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            40566..>41750
                     /locus_tag="ACFB78_000232"
     CDS             40566..>41750
                     /locus_tag="ACFB78_000232"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC1268321.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVK"
BASE COUNT         9998 a        11482 c        10527 g         9743 t
ORIGIN      
        1 tcaaatcaat atcccccttc cgacaattaa aaacggcttc cgcaaggaaa aaagcggcaa
       61 atctgacaaa caggtacaat tcctgataaa atctaacact ttgctacatt aatttattca
      121 atgcttttga tgccacgcga cacggtatca aaaacacgcc gtttttacct taaccatcac
      181 aagtaatgca aatctatgat taaaatcaaa aaaggtctaa atctgcccat cgcgggcaga
      241 ccggagcaag tcatttatga cggcccggcc attaccgaag tcgcgttgct tggcgaagaa
      301 tatgtcggca tgcgcccctc gatgaaaatc aaggaaggtg aagccgtcaa aaaaggccaa
      361 gtgctgtttg aagacaaaaa gaatccgggc gtagtattta ctgcgccggc ttcaggcaaa
      421 atcgccgcta ttcaccgtgg cgaaaagcgc gtacttcagt cagtcgtgat tgccgttgaa
      481 ggcaacgacg aaatcgagtt cgaacgctac gtacctgaag cgctggcaaa attgagcagc
      541 gaaaaagtgc gccgcaacct gattcaatca ggcttatgga ctgcgcttcg cacccgtccg
      601 ttcagcaaaa tccctgccat agatgccgag ccgttcgcca tcttcgtcaa tgcgatggac
      661 accaatccgc tggctgccga ccctacggtc atcatcaaag aagccgccga agacttcaaa
      721 cgcggcctgt tggtattgag ccgcctgacc gaacgtaaaa tccatgtgtg taaagcagca
      781 ggcgcagacg tgccgtctga aaatgctgcc aatatcgaaa cacatgaatt tggcggcccg
      841 catcctgccg gcttgagtgg cacgcacatt catttcatcg agccagtcgg cgcgaataaa
      901 accgtgtgga ccatcaatta tcaagacgtg attgctatcg gacgtttgtt cgtaacaggc
      961 cgtctgaata ccgagcgcgt ggttgccttg ggcggcctgc aagtcaacaa accgcgcctc
     1021 ttgcgtaccg ttttgggtgc gaaggtgtct caacttaccg ccggcgaatt ggttgacgcg
     1081 gacaaccgcg tgatttccgg ttcggtattg aacggtgcga ttgcacaagg cgcgcatgat
     1141 tatttgggac gctaccacaa tcagatttcc gttatcgaag aaggccgcgg caaagagctg
     1201 ttcggctggg ttgcgccgca gccggacaaa tactccatca cgcgcaccac tctcggccat
     1261 ttcctaaaaa acaaactctt caagttcacg acagccgtca acggcggcga ccgcgccatg
     1321 gtaccgatcg gcacttatga gcgcgtaatg ccgttggaca tcctgcctac cttgcttttg
     1381 cgcgatttaa tcgtcggcga taccgacagc gcgcaggctt tgggttgctt ggaattggac
     1441 gaagaagacc tcgctttgtg cagcttcgtc tgcccgggca aatacgaata cggcccgctg
     1501 ttgcgcaaag tgctggaaac cattgagaag gaaggctgat tatgggctta aaacattttc
     1561 tggaaaaaat cgaaccgcac ttcctgccgg gcggcaaaca tgaaaaatgg tatgccctct
     1621 atgaagctgc ggcgacgatt ttctatacat ccggcgcggt aacgcgcaaa gcggcgcacg
     1681 tccgcgacgc gctcgactcc aaacgcatga tgattttggt gtggctggct ttgttccccg
     1741 ccatgtttta cggcatgtac aacgtcggcg cacaggcatt cggtgcctta acgcccgatt
     1801 tgctgcaaca aagcatcgcc aacgactggc attacgccct tgccaacgct ttgggcatca
     1861 atatgtcgcc tgaagcgggc gtgttgggca aaatgctgtt cggcgcgatt tacttcctgc
     1921 cgatttacgc gaccgtattt attgtgggcg gcttctggga agtcttgttc gcatccgtac
     1981 gcaaacacga aatcaacgaa ggtttcttcg ttacttcgat tctgtttgcc ttaatcgttc
     2041 cgcccacgct gccgctgtgg caggcggctt tgggtatttc tttcggcgtt gtggttgcga
     2101 aagaagtatt cggcggtaca ggtaaaaact tcatgaaccc tgcgctggca ggccgcgcct
     2161 tcctgttctt cgcctacccc gccaacttga gcggcgatgc ggtttggacg gcggttgacg
     2221 gctattccgg cgcaaccgcg ctggcgcaat gggcggcaca cggtgcagac ggcctgaaaa
     2281 acgccgtaac cggtcaaacc atcacttgga tggacgcgtt tatcggcaaa ctgcccggct
     2341 ccatcggcga agtctccact ttggcactct taatcggcgg cgcgtttatc gtgtttgccc
     2401 gcatcgcttc ttggcgcatt attgccggcg tgatgatcgg tatgattgcg atgtcttcgc
     2461 tgtttaactt catcggttct gacaccaacg ctatgtttgc tatgccttgg tactggcact
     2521 tggtggtcgg cggcttcgcc atcggtatgc tgtttatggc gaccgacccc gtttccgctt
     2581 cctttaccaa tgtcggcaaa tggtggtacg gcgcgcttat cggcgtaatg tgcgtgttaa
     2641 tccgcgtggc caatccggct taccccgaag gcatgatgtt ggcgattctg tttgccaacc
     2701 tgtttgcccc gattttcgac tatttcgtcg cacaagcgaa catcaaacgc agaaaggcgc
     2761 gcagcaatgg ctaagaaatt cgataaagac agcttcagcg gcacgctgat tgtcgtgttg
     2821 gcggtcagcc tgatttgctc ggtcatcgtt gccggtgcgg tcgtcggctt gaaacccatc
     2881 caagaaaaac aaaaactcca agacaaacaa ggctatattt tgagcgtggc cggtttgatg
     2941 gataaggaca ccgacatcgg taaaaccttt gccgagcgta tcgagcaacg cgtggtcgat
     3001 ttggcgaccg gcgaatatgt gaaagacgcg ccgaaagact tcagcgcgcg catcgcaggc
     3061 aaagaccccg cgcaaagcat ccgcatcaaa cctgaagacg atttggcagg catcaaaagc
     3121 cgcgccaaat acaccgaggt ttatttggta aaaggcgaag acggcaaaat cgggcaaatc
     3181 atcctgccga tgcacggcaa cggtttgtgg tcggtgatgt acggcttcgt cgccatccga
     3241 cccgacggca acaccatcaa cggcattacc tactacgaac aaggcgaaac cccgggcttg
     3301 ggcggcgaaa tcggcaatcc tttgtggcag caaaaattcg tcggcaaaaa attgtttgac
     3361 ggacaaggca aactcgccct gcacgtgggc aaaggcgcgg gttcggacaa agaacacggc
     3421 gtagacgccc tctccggcgc gtcgctgact tccaaaggcg tgcaaggttc gttcgcctac
     3481 tggttcggcg aaaacggcta tatcccctac ctgaacaaat tgaaatcagc aggagtgcaa
     3541 taatggctga tatgaaacgc ttgaaacatt tgatgttctc accctttatc gacaacaacc
     3601 cgattgcctt gcaggttttg ggtatttgtt cggctttggc ggttaccacc aaacttcaga
     3661 cggccatcgt aatgggtatt tccgtagctt tggtaaccgg tttttccagc ttcttcattt
     3721 cgctggtgcg caactacatc cccaacagca tccgcatcat cgtgcaaatg gcgattatcg
     3781 cgtcgctggt tacgctggtc gaccaactgc ttcaggcatt tgcctacgaa ttgtccaagc
     3841 agctttccgt attcgtcggc ctgattatta ccaactgtat agtgatgggc cgtgccgaag
     3901 catttgcgat gaaagagccg ccgctggaaa gcctgatcga cggcatcggc aacggtgcgg
     3961 gctacgggat gatgctgctt gtcgtcgcca ccgtccgcga actgatcggc tcaggcaaac
     4021 ttttaggtta taccgtcttt caaaccgtac aggacggcgg ctggtatcag accaacggcc
     4081 tcttcctact cgcccccagc gcgttcttca tcatcggctt tttgatttgg ggactgcgta
     4141 cttggaaacc cgaacaggcg gaggaataag ctatggaaca ctatttgagc ctcttcatca
     4201 aatccgtctt cattgaaaat atggcgctat ccttcttttt gggtatgtgt acgtttttgg
     4261 cggtatccaa aaaagtatcc accgcattcg gtttgggtgt ggcggtaatt ttcgtactcg
     4321 ggctgtccgt ccccgccaac caattggttt actcgctgct caaagacggc gcgattgtcg
     4381 aaggcgtgga tttgaccttt ttgaaattca tcacctttat cggcgtgatt gccgctttgg
     4441 tgcagatttt ggaaatgttc ttggacaaat tcgtccccgc cctctataac gcgctgggca
     4501 tctacctgcc tctgattacc gtaaactgcg cgattttcgg cgccgtatcg tttatggcgc
     4561 aacgcgaata cgacttcggc gagtccgtcg tgtacggctt cggcgcgggt ttgggctgga
     4621 tgttggcgat tgtcgctttg gcgggcatta ccgaaaaaat gaaatattcg gacgctccca
     4681 aaggcctcaa aggcttgggc attaccttta tcgccgccgg cctgatggca atggcgttta
     4741 tgtcgttctc cggcatccag ttataagaaa gggaccggta tggagattat tttaggtatc
     4801 gtgatgttta ccgtcatcgt cttagctttg gcactgatga ttctgtttgc caaatccaag
     4861 ttggtcagcg aaggcgacat caccatcaaa gtcaatgatg aaaaagaact gactatgccc
     4921 gccggcggca aactcttggg cgcgcttgcc agccaaggca tctttgtccc ctccgcctgc
     4981 ggtggcggcg gttcgtgcgg acaatgccgc gttgtcgtga aaagcggcgg cggcgacatt
     5041 ctgccgaccg agctgtccca catcagcaaa cgcgaagcac gcgaaggctg ccgtttgtcc
     5101 tgccaagtca atgtcaaaac cgacatggac atcgaagtac ccgaagaagt gttcggcgtg
     5161 aaaaaatggg aatgcaccgt catttccaac gacaacaaag caacgttcat taaagaactc
     5221 aagcttgcca ttcccgaagg cgaagaagtt cctttccgcg ccggcggcta cattcaaatc
     5281 gaagccccgc cgcacaccgt tgcctacaaa gacttcgaca ttcccaagga atatcacgaa
     5341 gactgggaca aatacaatct gtggcaatac gtttccaaag tggacgagcc gattttgcgc
     5401 gcctactcta tggcttcgta tcctgaagaa aaaggcatca ttatgctgaa cgtgcgtatc
     5461 gccacgcctc ccccgcgcgt acccgatgcg cctccgggac aaatgtcgtc ctacatctgg
     5521 tcgctcaaac ccggcgacaa agttacgatt tccggcccgt tcggcgaatt tttcgccaaa
     5581 gacaccgatg ccgaaatggt atttatcggc ggcggtgcgg gtatggcccc gatgcgttcc
     5641 cacattttcg accagttgaa acgtttgcac tccaaacgca aaatcacctt ctggtacggc
     5701 gcacgctcca aacgcgaaat gttctatgtc gaagacttcg accaactcgc ggcagaattc
     5761 ccgaacttca cttggcacgt cgccctgtcc gacccattgc ctgaagacaa ctgggacggc
     5821 tacacgggct tcatccacaa cgtggtttac gaaaaccacc tgaaaaacca cgaagcaccg
     5881 gaagactgcg aattttatat gtgcggcccg ccgattatga accagtccgt catcaaaatg
     5941 ctcaaagact tgggcgtgga agacgaaaac atcctcttgg acgatttcgg cggttaaata
     6001 ttcggcttca aacacaaaag gaagaaaccc cggtttcttc cttttttatc cgatgccgtc
     6061 cgaacaccct tcagacggca tccgcccata cccgtatcaa tatcgttttt taccctgcgg
     6121 cggatgtacg gttaaatatt ttgacgaaaa aacaaagaag tatacttctt tttagttatg
     6181 gttgattcca taactaaaaa agtatcgata aacccatttt gcgaggtacg gataatgtct
     6241 gacagctttg aaaacagccc cgaatacgat gattggattg aatcgggcgg tcgtgatgaa
     6301 gattacgaat actactacaa caaatggcaa cgcaggacgc gctgaccatt aaaccctgac
     6361 aaaccggttt gtaaaacacc ggtttgtttt ttattaccgc cacccaaacg acgggcgatg
     6421 ccgcccgaac gcccttcaaa cggcatcggc atagaaacat accggccgtt ttgcgatata
     6481 attcaagcca tttgccaaaa ccgtcaaaca ctatgccgtc tgaaacacgc ctgccgaacc
     6541 ttatccgcgc cttgatattt gccctgggtt tcatcttcct gaacgcctgt tcggaacaaa
     6601 ccgcgcaaac cgttaccctg caaggcgaaa cgatgggtac gacctatacc gtcaaatacc
     6661 tttcaaataa tcgggacaaa ctcccctccc ctgccaaaat acaaaagcgc attgatgatg
     6721 cgcttaaaga agtcaaccgg cagatgtcca cctaccagac cgattccgaa atcagccggt
     6781 tcaaccaaca cacagccggc aagcccctcc gcatttcaag cgatttcgca cacgttaccg
     6841 ccgaagccgt ccgcctgaac cgcctgactc acggcgcact ggacgtaacc gtcggccctt
     6901 tggtcaacct ttgggggttc ggccccgaca aatccgttac ccgtgaaccg tcgccggaac
     6961 aaatcaaaca ggcggcatct tatacgggca tagacaaaat cattttgcaa caaggcaaag
     7021 attacgcttc cttgagcaaa acccacccca aagcctattt ggatttatct tcgattgcca
     7081 aaggcttcgg cgttgataaa gttgcgggcg aactggaaaa atacggcatt caaaattatc
     7141 tggtcgaaat cggcggcgag ttgcacggca aaggcaaaaa tgcgcacggc gaaccgtggc
     7201 gcatcggtat agagcaaccc aatatcatcc aaggcggcaa tacgcagatt atcgtcccgc
     7261 tgaacaaccg ttcgcttgcc acttccggcg attaccgtat tttccacgtc gataaaaacg
     7321 gcaaacgcct ctcccacatc atcaatccca acaacaaacg acccatcagc cacaacctcg
     7381 cctccatcag cgtggtctca gacagtgcaa tgacggcgga cggtttatcc acaggattat
     7441 ttgttttagg cgaaaccgaa gccttaaggc tggcagaaca agaaaaactc gctgttttcc
     7501 taattgtccg ggataaggac ggctaccgca ccgccatgtc ttccgaattt gccaagctgc
     7561 tccgctaaac atacaaaccg attaggaaac accatgaaaa ccctgctcct caccttcggc
     7621 atcttcctga ccgtcatcat cggtatggcg gtcggctata ttttctccaa acgcaccatc
     7681 aaaggcagtt gcggcggcat taccgcttta ggtatgaaaa aaatgtgcga ctgcgacaca
     7741 ccttgcgaca ccctgcaaaa aaagctggat gaagaaaaac aggcaggcgg catacgggtt
     7801 gaccgttaac gccgaaacaa aatgccgcct gaagcccggc cggcgggttc agacggcatt
     7861 gccgctttac ttcgatcggt ttatacgcaa aacgcgcttc aggaaataaa tcagattatt
     7921 ccccagacgc cttcaggctt ccttctgtgc caccgtaacc atagccgggc gcaacacacg
     7981 gtcggacagg gtataaccct tcttcatcac accaaccacg gtattgggtt cctgctcact
     8041 ggccaccgcc tgcatcgcct gatggatatt cggatcgagc ttatcgcccg ctttaggatt
     8101 gatttccctg atttgcgtgg catcaaacgc cttctgcaac tcattcaaag tcatctgcac
     8161 acccattttc agcgcatcga aattaccgct ctgatccaaa agcgccattt ccagataatc
     8221 cttgaccggc aacatttcca cggcaaactt ctgtccggcg aacttgtgcg tatcggcaat
     8281 ttcctgctgg tggcggcggc gcaggttttg ctcgtttgcc aaagcgcgca gttgttcgtc
     8341 tttcaactgc gcttccaact cggcaatccg cgcctgcaaa tcctcataag ccggctcggc
     8401 ggcagcctgt tcctgtacac cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc
     8461 cacattttca accgcttctt cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc
     8521 ctcaaaacaa attggaaatc aaaatccggt tattacccga gcaagataag gacattttca
     8581 agaaacttca agcaaaggca ggaaatttca catccgccgc cgaataccct accgcaaaaa
     8641 ccatataaac ggtaagatac cgccgacctt atgcttttca gacggcatcc attatgaaaa
     8701 aaaaccattt gaacacaacc ggtttcgacc tctggcacac catccgcgaa gaaaccgcgg
     8761 ccgctgccgc cgccgaaccg atgctggcaa gttttttgca ccaaaccgtg ttgcgccacg
     8821 agtccctcgg ctccgtcctt gcctaccacc tttccagcaa actcggcagc ccgattatgg
     8881 acgtgcgcgc gctgtttgaa atttaccagc aggcattggg cagcgacacc caaatcagca
     8941 aatgtgtcga agcagactta aaagccatct acgaacgcga tcccgcctgc gacgaatatt
     9001 cgctgccgct tttatatttc aaaggcttcc acgcgattca ggcacaccgc atcaaccacc
     9061 ggctgtatct cgacggacgc aaaacgctgg cgtatttcct acaaaaccgt atgtccgaag
     9121 tattcggcgt ggacatccat cccgccgccc gtttaggata cgggctgatg ctcgaccacg
     9181 ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa caatatttcg attttgcacg
     9241 gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg ccaccccaaa atcggcgacg
     9301 gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat ccgcatcggc agcaatgcca
     9361 aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc gtccatcacg gttgtcggcg
     9421 tacccgccaa acccgtggcg cgatcgctca aaaccccgtc ggcggatatg gatcaaaata
     9481 tccagtttgc cgaaatcgac tttatgattt gaatatccgc accaatgccg tctgaaacgc
     9541 caaacgggct tcagacggca ttgcccgctt caagcctgcg ccttaatatg ccgccgatgc
     9601 cggcacttat cagtcggaca aaatgccttt tattcgatac gcccgattcc cacgcaaccg
     9661 gaaataaccg gtttccttgt aaacgggcgg agtcgggcga tgattcggac aaagcctatt
     9721 tcaaaagcaa aatcagcagc aaaacaaccg ctatcgctgc cagccacagg ctttgccgct
     9781 gctgcacttt gaccaaatgg atataggcat cgcgcatttc ctgccggcgc gcctcatcga
     9841 ccaaggcatt gattttgcgc ggcagggaag ggatgatttg cgcccagtcg ggggcttcgt
     9901 ttttgaggtt gcgccaaagg gctttgggac cgacctgccc gttcatccat ttcaccaaaa
     9961 acggtttggc ggttttccac aagtccaaat cgggatcgag ttggcggccc aagccttcga
    10021 tgttgagcag cgttttttgc agcaatacca gctgcggctg gatttcgaca ttgaagcggc
    10081 ggctgacttc aaacaggcgc atcagtacca agccgaagga aatctgcgaa atcggtttgt
    10141 tgaacacagg ctcgcacacg gcgcgaaccg ccgcttccaa ctcttccgca cgcgtgtcgg
    10201 caggcaccca gcccgattcg acgtgggcgg tggcgacacg tcggtaatcg cggttgaaaa
    10261 aggcgaggaa attgatggcg agatagcgtt tgtcgtaatc ggtcagcgta ccgacgatgc
    10321 cgaaatcaag ggcgatatag cggttgtcgg cggcaaccaa aatattgccg gggtgcatat
    10381 ccgcgtggaa aaagccgtcg cggaaaactt gcgtgaaaaa gatttccacg ccgtaatcgg
    10441 caagtttgtg caaatcgatg ccgtctgctt tgagtttggc gatgtcggaa accggcgtgc
    10501 cgtccatcca ttcaatcgtc agcacgtcgc ttgtgcagta gtcgtaaaac accttgggca
    10561 caatcagcat attgctgttt tggaaattgc gcccgagttg tcctgcattg gcggcttcgc
    10621 gcatcaaatc caattcgtcg tgcaggtatt tgtcgaactc cgccaccact tcgcgcggct
    10681 tcagacgctt gccgtctgaa aacaagcgtt cgacccaagc cgcgccgaaa cgcatcagcg
    10741 acaagtcttg ttcgataagg ggcaagaggt tggggcgcaa aactttgacc gccactcgtt
    10801 cgcccgaatg caggcgggct ttgtgtactt gggcgatgga cgcgctggcg acgggctcgg
    10861 tttcaaattc cgcatacagc ttttcgatgg gctgacccag cgatttttcg atttgttcac
    10921 gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag ctttgccagt tcgacggcgt
    10981 aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg cccgaacttg atgaaaatcg
    11041 gccccaggct ttccaaggca aggcgcagac ggacggcagg cggttcgttt ttcaatttgg
    11101 acgactgcgg catcattttc agcagagcgc atatccaacc gctacccatc agcgaaacgc
    11161 acagacctgc cagccgatag cggtaaaaag tcccgacaat gaccgtcagg cgtttcaacc
    11221 atttcatata taaaatcccg aatacagcat ttatcggaca atcaaaaaat cttaccgagc
    11281 agatagccca aaaaggcaat cagcaacccg aaaaccagcg gtttgatatt gcggggcggc
    11341 tcgggttgcg aagatgcgga aatatcatct tcccgcccgc cgtgaagttc aagatattcg
    11401 ggcatttccc catccgcgcc tcctccttcg ccctccccct cctccggcgg cacgaccgtt
    11461 tccaactcca tatacgggta ataatatcca aacccaaata gctgaaataa tcatgcaact
    11521 cttcccgttt cgcccaagtg catttttcct caaccagcac ttgcggtttg gaatcgagcg
    11581 attcgatcaa tccttccgcc ttttcttcgc cgagattctc cttaaggcac tcccggataa
    11641 gcccgcgatc cacttggggc gggtaggaaa catatacatc gtacaaatcg tttcggtaat
    11701 ctttactcat ctcaatcata agggaacaaa tacgcctgat accgttccgc ccgttccttt
    11761 cgggaaaccc gattcatctt caagcaatcg gattatatgt aaattcatcc cgataatata
    11821 ttttccaaaa gtaaaaatgc cgtccgaacg ttcagacggc ataacacaaa ccagccggtc
    11881 acacggaaaa cgacgatccg caaccgcagg ttgtttccgc gttcggattg cggatgacga
    11941 actgcgaacc ctgcagactt tccgtatagt cgatttccgc accgaccaga tattgatagc
    12001 tcatcggatc gaccaaaaag accaaaccgt ttttctcaat ttcaaaatcg tcgtcgtttt
    12061 tgatttcgtc aaaagtaaat ccgtactgga aacccgaaca gccgccgccg ttgacaaaaa
    12121 cccgcaattt caaatcggga ttgttttctt cggcaatcaa atcggcaact ttggcacagc
    12181 agctgtcagt aaaaataata gggctttcgt ccgacatagt gttattcctt aatataatat
    12241 tttctgcatt ctctctcaac cccccttcaa aggcaagcag ggcttcgcct ttgaaaaagg
    12301 aattgtccca atagataagt ttgaccgcct gcaatttcaa gccccaaact caaaccgtcc
    12361 tgccgaacca ctcgacacgc ccgataatct ccacatcatc ggtcaagtcg ttcaaattga
    12421 tttcaaaagc aggataagcc tcgttggcgg aaatgacatt gataatcccg ccggggacga
    12481 tttgcaggcg tttgaccaac agattttcat taatccgcaa cacatacagt ccgtctctcg
    12541 gcgtattttc cccatgattg accaaaatcg aatctccgtc attcaaaacc ccctccatcg
    12601 aatccccctt gacggaaatt acagacaggt ttttcgtatc tcgggtaaca taattctcaa
    12661 tccaatgccg tctgaacgcc attgtaaata ccggttcctc atgaccgaca aactgcccgt
    12721 atcccgcagc cgcccgaata tcatatctcg gcacaaagac aaactcgtcc gtatcaactt
    12781 catttcccaa ggtatcgtaa gcaagggatt tttttggggc ttcatccgga aacggattac
    12841 cctcccctgt cagcagccaa tcgatactac accccttcaa ctgcttaatt tttttcaatg
    12901 tttcagactt cggcagaccg ccttcattcc atattctgct gaagcccgca atcgtcattt
    12961 caatatcgga tgcgattttt gcctgcctcg cttcactttt ccataaaaaa accagtctgt
    13021 ctttaaaggt ttccataaat atccttccgc tccccttcat tcaatcatct aaagtaaaca
    13081 tgaaatatat tttacattag ctatcccaaa aacggaataa ctatgataaa ctaaatttaa
    13141 attatgattt ttcttatcca atacataatt tttcagattt tccataggtt taatcccgaa
    13201 ttgacagaaa aaggatagaa aaatgaagaa aaccagcaaa tatcttatct atactgcggc
    13261 atttacctca ttctgctttg ccttccaaga aaaccgttct gaagccaaac agcccgacat
    13321 cactttatcc gcatccctgt gcgaacaatt caacatgctg aacgccaaag atatggatac
    13381 agaacaagtc tccctttcca aagaatgcga catcatcgag tcttcacacg actgggaaaa
    13441 agagtacggc aacttgaacg aacaggaaat gctcgccggc gtcgtctatg aataaacctg
    13501 cctgccattt gaaacattat gcttgaatgc attggagcca aatgtattaa atcaaatata
    13561 aaaccaatat attcataaag ttatatactt atggccatgc tgtagcttga aacagcccgc
    13621 cgcccgccct atttacagcc catcgggaca aaatgttcca acatatttct caaaataagc
    13681 aaaatcaaat aggagtatcc acatggcaaa agtaatcggt atcgacttag gtacaaccaa
    13741 ctcttgtttg gccatttccg aaaacggtca aaccaaagtc atcgaaaacg cagaaggcgc
    13801 acgcaccacg ccgtccatta tcgcttattt ggacggcggc gaaatcctcg tcggcgcgcc
    13861 tgccaaacgc caagcggtaa ccaacgccaa aaacactatt tacgccgcca aacgtttgat
    13921 cggtcacaaa tttgaagaca aagaagtcca acgcgacatc gaatctatgc ctttcgaaat
    13981 catcaaagcc gacaacggcg acgcatgggt aaaagcacaa ggcaaagagc tgtctcctcc
    14041 tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc gccgaagctt acttgggcga
    14101 aaaagtaacc gaagccgtga ttaccgtccc tgcctacttc aacgacagcc aacgtcaagc
    14161 caccaaagac gcaggccgta tcgccggttt ggacgtaaaa cgcatcatca acgagccgac
    14221 cgcagccgct ttggcattcg gtatggacaa aggcgacaac aaagaccgca aaatagccgt
    14281 atatgacttg ggcggcggta ccttcgatat ttccatcatc gaaatcgcca acctcgacgg
    14341 cgacaaacaa tttgaagtat tggctaccaa cggcgatact ttcttgggcg gtgaagactt
    14401 cgaccaacgc ttgattgact acatcattga cgagtttaaa aaagaacaag gcattgattt
    14461 gaaacaagac gtaatggctc tgcaacgtct gaaagaagct gccgaaaaag ccaaaatcga
    14521 attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac attaccatgg acgcaaccgg
    14581 cccgaaacac ttggcaatga aaattacccg cgccaaattc gagagcctgg ttgaagacct
    14641 gattgcccgc tctatcgagc cttgccgcac cgcattgaaa gatgccggct tgagcaccgg
    14701 cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt atgccgaaag tacaagaagc
    14761 cgttaaagac ttcttcggca aagaaccgcg caaagacgtg aaccctgacg aagccgttgc
    14821 cgtaggtgca gcgattcaag gcgaagtatt gagcggcggc cgcagcgacg tattgctgct
    14881 ggacgtaacc cctctgtcct tgggtatcga aaccatgggt ggtgtgatga ccaagctgat
    14941 tcaaaagaac actactattc cgactaaagc gtctcaagtg ttctctactg ccgaagacaa
    15001 ccaaagcgca gtaaccatcc acgtactgca aggcgaacgc gaacgcgctt ctgccaacaa
    15061 atctttaggt caattcaact tgggcgacat cgcacccgca ccacgcggta tgccgcaaat
    15121 tgaagttact ttcgacatcg acgccaatgg tatcttgcac gtttccgcca aagacaaagg
    15181 cactggcaaa gcggccaaca ttaccatcca aggttcttca ggtttgagcg aagaagaaat
    15241 cgaacgcatg gtgaaagatg ccgaagccaa tgccgaggaa gataaaaagc tgactgaatt
    15301 ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc gtgaaaaaat ctttggcgga
    15361 ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc gaagccgcgc tgaaagaagc
    15421 cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat gccaaagccg aagcactggg
    15481 cacagccagc caaaaactgg gcgaaatggt ttacgcgcaa gcgcaagccg aagcccaagc
    15541 cggcgagggc gcacaagcca atgcttctgc aaagaaagac gatgatgtcg tagatgccga
    15601 ctttgaagaa gtaaaagacg acaaaaaata attgatgccg tctgaaaaaa acgcgaacca
    15661 ttcggttcgc gtttttttca attgagataa aagacaatag catgacagag attccaaatt
    15721 catctaccgt gatattccca agcccaagtt ccaactgttg catcggttat ctggaaattt
    15781 ttcatatcat tttcttattt acttaaattt tttaataaga taataaataa taattattat
    15841 catgagctaa gaaatgaaac taaatactct cacatgggct ttgatgaccg ttttttccgt
    15901 tgcgccatct tgggcagaac aaccggcaaa tactgaagaa atacaacccg tcaaaacctt
    15961 ctccccgccc aaaccgattg caccgaccgc cgcacaaggc tatttccccg aaaaccaatt
    16021 cgaccgctcc gaccgcagcg attattactt tgttaccgaa aacatagacc aagccttccg
    16081 tccgctgaag gcaaacagca gtttttacgg caaaagcttt tacaactccg ttaccgcgca
    16141 agcactcggg gcgaaggtat acggcgtagc caacctcaat cgcaccaagg caaacggcta
    16201 caaagatggc ggtgggcgcg acaccgattg gaaatacagc cgcttcaatc aggctttggt
    16261 actcggtttc gtgccgtctg aaaatcaaga ataccgcctc acttatctgc acgacgacat
    16321 caacaacgac cgccagccgc aggtcgtcaa cgacgcattg gacaccgaac gccacatctc
    16381 caaactcaac gtgcgttggg gcaatgccga tttgagcaat acggtcagcg cggaagcagg
    16441 cgtcatcaaa ctcaaacgcc atgccgacaa ttactccttg cgaccaaaca acacgccgca
    16501 gcaagtgttc gtagaacttg accgcaaagt gtatgatttc tcgctcaaac acgatgcgga
    16561 tttcggcaaa ttccacaaca cggcagccgt cagctaccgc aacgacagcc aaaacggcga
    16621 acgcaacacc cacactgcca tgtgcgattt cctcaacggc taccgtttcg ccgatgtgca
    16681 catcgaccgc tggtgcattg ccgatacctt atcttacaag tttgacgacc gacacaaact
    16741 gggcttgggc ttaagctacg aactcaacga agcggacatt cgcaaaaata cggcacagcc
    16801 tgaaaaccca ataaaacccg gtttcccctt cgcctcatca caacaaatct ggaaaaccca
    16861 ttacggctac gacttcaacg gcaaagtgcg ccgtcatgct ttgtcgggcg aactcaaata
    16921 cgatttcacg ccgtctgaaa cgcaaaaata cagcgtttcc ctcgcgcatt tggaacgcat
    16981 cggcgacaac accgaacgct tcaactcgct tgctgccatc gtgcaaaacc gtatgagcgg
    17041 catgctgatg aaccaaaatc cagccgccgc cattgcaggc aatccccctg ctgaaaactg
    17101 aaaaacacaa ccgcatcagg ctcaccgccg acagccgcaa cgactactac aacggctaca
    17161 tgaactcgct cgcaggcgcg ggctggaacg tgggcggcac gcttgtggcg gacaaagtca
    17221 aagacctgat tatttttgac cgcgcacacg gacaaagcgg cacagcttcc aaagacggcg
    17281 gcatcatcac acgcaacgtg gacgcacgat tgtttaccgc gcaggcctac gcgcgttaca
    17341 acttcaatcc gcattgggcg gcaggcatca aagccgccta caactacgga cacaacgaaa
    17401 ccgacggcag gccgccctat caaatccgtc cgtttgaagc cgccgtccaa gccgactaca
    17461 aaaactactt tgcccacggc agctacaaca tcggcgcggc aacacgcttt gtcgccaaac
    17521 aaacgcgcgg tgattttgat atggcaagcg gtctgggcat agacaaacgc gaagccgcca
    17581 aaggctttac cgttgccgac gtgtacgcag gcgtaaacat caaagacaaa tacggcttgc
    17641 gcttgggcgt gaacaacgta ttcaacaaaa aatacgtcga atacatcagc ggcgaccacg
    17701 tcctcgccct atcccccagc gtagtgtatg caccgggcag gacatattgg ttgagtttgc
    17761 atgcggcatt ttaaaaccat gccgtctgaa aaatacctgc attggcttat taagaattag
    17821 gagataaaaa atggatatga aaagacgcga tttcttaaaa atgaccgccg cgctggcagc
    17881 cgcaggcgtt tcgccttctc tgcttgctgc cggtaaagag caatttaccg tgtacggcgc
    17941 accggcaatg cccagcgtta ccattgccgt agcggcgttg caaggtaaac tggcgaaaca
    18001 ggcggacgtg tcgctgaaaa tctggcgttc gcccgaccaa ctgcgcgcgg gcgtggcaag
    18061 cgggcaattt aaagtgatga tgagtccgag caatgtcggc gtaaacctgc gcaaccaagg
    18121 gcagaaagtc ggcatggtga atattttgac caaaggcatc acgcagttgg tctgcaaagg
    18181 cagcgcgatt gcctcgccgc aggatttggt cggcaaaaaa atcctcgtgc cgtttaaaaa
    18241 cgatatgccc gacatcgtgc tgcaagcctt gttgaaaaaa ctgaaaatcg acgcacacaa
    18301 agtcagcatt acctacgctg ccacaccgcc cgaagcagtc ggactatttc caagtaaagg
    18361 ttaccatgcc gtcatcctgc ccgaaccgat ggcaaccgcc agcctactga aaggcaaaac
    18421 cataggtata aacgtcgtgc acggttttga tttggtaaaa gcatgggggc aggcgtttgg
    18481 caccaaaccg ctgattccga tggcaggtat catcgccaac gaagaatatt tccacgcaca
    18541 caaggcgcag ttcgacatct tccatcaaga tttgaaaaac gcgctcaact ggatattggc
    18601 caaccgccaa aacgcggcga aaatcggcaa aaactaccta cccgcccccg aacccgccct
    18661 agtcatgggc ttggacggcg cacggctgac ggtaagcaaa ggcagcgaag tgaaaaacga
    18721 gattttgaag ttttacgaaa tcctgatgca gttcaacccg gaacttttgg gcggcaagct
    18781 gccggataac gggttcttct tggcttaaag ggaaagaggc cgtctgaaaa gggtagggtg
    18841 gttgtgaata cctgatttat catttttcag acggcctatt aaggcattgt cagcggcgtg
    18901 ggtcggattc ttgaatccga catttcggct aaacggggct gtcggataca agtatccgac
    18961 ctactctttt cagacggcct atcagataat agttcgaaca gtacacaaag agccgtcgga
    19021 ttgcctgaaa tgttgggtca tgacccaacc tacgcttact ggggaaacgg gatcgaagtg
    19081 tgaggtatgt ggcggggttg tttaataatg aaattttata actatttgat agggtggtaa
    19141 tgctatgaat gaagataaat atagggaaaa tatgacatgg gatgaaattg aaaatgtagt
    19201 aggaacttta gtttctgaag aaagaatgct taaatccaat agctttttaa gtattaaaaa
    19261 tagtaagaat aggttggctt aatcacaacc ataacccaac gaaaatacta aaattccaag
    19321 ctaaggctat cctttccaaa ccatccccat caaccaaacc atgattaaaa ccgacaaaat
    19381 ccgcaaaccg cagcctgcgc tgttttacat catcgactac ctttggagcg gctttgccgg
    19441 tctgagcgtg gcgatggtgg tggtggcgtt gtgggcgtgg ggcagcgccg tgttcggcga
    19501 gtttatgctg cctgcgccgg tagaggtgtt tcaaaaatct ttggatttat tgaaacattt
    19561 tcaggaaaat gaaatcggga tttcgctgtg gcggtcggta gtgggtattt cggttgcttt
    19621 gatagcagga ttggcggcgg ggctggtggc gggcagtttt aagacggcga tggcgttgct
    19681 taagcctgtg attacgattt tgttagcaat gccgccgatt atttgggtgg tgatggcgtt
    19741 gttttggttc gggttcggca atccgagcgt gttgtttacc atcattgtgt tggttgcgcc
    19801 gctgacgttt gcgagtgcgg cggtcggaat ggcgagcgtg aacaagcagc atgaggagtt
    19861 gtttgacgct tataaattag gccgtctgaa aaaaatccgc tatctgtata tcccgcatct
    19921 gacgggctat gtgatttcca gcgtcggcgt ggcggtggcg acgggggtga aggcggtgat
    19981 catggcggaa ctcttaggcg cgagcaaagg cgtgggcgcg cggattgcgg acgcgagggc
    20041 aatgctgtag acttcgacgg taatggctta tgtggtgttg gtcatggtat ttgtgtcgct
    20101 gtttgaatac ctgattccaa gcctttgtaa attttgttta tgccgtggag gagatgatgc
    20161 tctgtcttga aaacgtgcgt tttgaaatcc tccgcgaccc catcgtgcgc gatttcagtt
    20221 tgaacctgca acagggcgaa gtgaaagcct tgttcgggcc gagcggctgt ggcaagacga
    20281 cggttttacg gctgattgcg ggcttggaaa cgccgaaatc gggcacgata cgtaatactt
    20341 tccacaaaac gggttttctg tttcaggaaa accgcctgcc ggaaaacctg accgcgatgc
    20401 agaatatcgc gatttttatg gacaacccag acgaaggcga aatcgtcgcg ctggcggcga
    20461 aagtcgggct gactgcgggc gatttgaaca aatatccgac cgaattgtcc ggcggcatgg
    20521 cgaaacgggt ggcgtttttg cgcctgctgc tgtgcggctg cgacctagcc ttgctggacg
    20581 aaccgtttgt cggtttggac cgcgatttgc gcgatatttt ggttgctatg ctggtggaaa
    20641 aaatcgagcg gcagggcatg gcgtgtatac tggtaacgca cgaccgcttc gaagccgcgc
    20701 gcctgagcca tgaaattatg ctgctttccg ctaagggcat gaacgtgcaa aacgtgataa
    20761 ccctgcccac gccgctgtcc gaacgcgatt cggcttttga agaagtcgtg gtggcaaggg
    20821 agtttcaggg gattcattat tatgagtgat gaggggattt gagtgtctga caaaccttac
    20881 ggttcgttgc cctctcccta accctctccc acggggagag ggttagcaag cctcaggctt
    20941 gcctctttag cgaaagatac gaatctgtta tccgaacgcg attcggtttt tgaaaaagcc
    21001 gtggtgccaa gggggcaggg gattcattat gaggtgcttt atgttttcga ctgtgattac
    21061 tgctgctgtt ttatatattg ctacagcagt agatttgttg gtaatactat taatattttt
    21121 tgctagagca aatactagaa aagaatatcg agatatttat atcggacaat atttaggttc
    21181 tgtaatttta atattagtta gtttatttct agcttttgtt ttgaattatg ttccggaaaa
    21241 atgggtgttg ggtttattag gtttaatacc gatttactta ggtattaaag ttgctattta
    21301 cgacgattgt gagggcgaaa aaagagctaa aaaagaattg gatgaaaaag ggttgtcaaa
    21361 attagtcggt attgttgctt tggttacagt tgctagttgt ggtgcagata atattggact
    21421 ttttgttcct tactttgtga ctttagatct tgtcgactta ttagttactc ttcttgtatt
    21481 tttaatattg atttttgttt tagtatatac agcacaaaga ttggctaata tttcaggtgt
    21541 tggtgaaatt gtagagaagt ttagtcgttg gataatggct gttatttata ttggtttagg
    21601 gttatttatt attattgaaa ataatacaat tcgaacaata atatcaataa tatgaatgac
    21661 aagggcattt gagtatctga caaaccttgc ggttcgtttc cctctcccta accctctccc
    21721 acaggagagg ggttcaggtt gctgaaaatc agagggcgaa ggattgggga tcaaatggaa
    21781 ggtaaaacct gaaaatgttc gggtttgaca gcccgattcc ctctccccgt gggagagggc
    21841 tagggagagg gcaagcaagc cgcaggcttg cctctttggc gaaaaataca accctgtccg
    21901 cccaataaat ccatacccga aagcatcttc atacagaatc aagccaagcg atgaataaat
    21961 tttttaccca ccctatgcgc ccgtttttcg tcggtgcggc agtacttgcc atactcggtg
    22021 cgttggtgtt ttttcatcaa ccccggcgct atcatcctgc accgccaaat tttcttggaa
    22081 cttatgctgc cggctgcata cggcggtttt ttgactaccg ctttgttgga ccggacgggt
    22141 ttttcaggca acctgaaacc tgccgctact ttgatggcgg tgttgttgct tgttgcggct
    22201 gttttattgc cgtttttacc gcaacttgcc gcatttttcg tcgccgccta ttggctggtg
    22261 ttgctgctgt tctgcgcctg gctgatttgg ctcgaccgca acaccgacaa cttcgctctg
    22321 ttgatgttac ttgccgcatt taccgttttt cagacggcct atgccgtcag cggcgatttg
    22381 aacttactgc gcgcgcaagt gcatttgaat atggcggcgg tcatgttcgt atccgtccgc
    22441 gtcagcgtcc ttttgggcac ggaaaccctg aaagaatgcc gtctgaaaga ccccgtattc
    22501 atccccaacg ttatctataa aaacatcgcc atcaccctgc tgctgcacgc cgccgccgaa
    22561 ctttggctgc ccgcgcaaac cgccggtttt actgcgcttg ccgtcggctt catcctgctc
    22621 gccaagctgc gcgaactgca ccatcacgaa ctcttacgca aacactacgt ccgcacttat
    22681 tacctgctcc agctctttgc cgccgcaggt tatctgtgga caggcgcggc gaaactgcaa
    22741 aacctgcccg cctccgcgcc cctgcacctg attaccctcg gcggcatgac gggtggcgtg
    22801 atgatggtgt ggctgactgc cggactgtgg cacagcggct ttaccaaact cgactacccg
    22861 aaactctgcc gcatcgccgt ctccatcctt ttcgcctccg ccgtttcgcg cgctgtttta
    22921 atgaacgtga atccgatatt cttcatcacc gttcccgaga ttctgaccgc cgccgtgttc
    22981 atgctttacc tgctgacgtt cgtaccgatt tttcgagcga acgcgtttac agacgatccg
    23041 gaataaaaat gccgtctgaa acggttttca gacggcatcg gattgccaat gatcaagcct
    23101 cctgcggatc gacatccacc gaccatcgga ttttgccgtc gcggttctgc tgcaacacct
    23161 gcacccacaa actcacggcg cggtgcaaat cttgtcggga tgttgattcg aggaaaactt
    23221 gcgcacgttc gcgttcggca aggcgcacca tcagcatcgg ggcggcaccg aaccgggaga
    23281 cgctttcggg caagagcggg gcgagggttt ctttggcggc gttgagaaat tccatcgcat
    23341 cggcaacacg cggcgcatcg gcacggacgg ccgtctgaaa accgaaaggc ggcatggcga
    23401 acatttgccg ttcgttcaat tcgttttcgg caaacacggc gtagtcctgc gctttgacgg
    23461 cggcgaagac agggtgttcg ggcagttggg tctgtatcag caccttgccg ggtttgtcgg
    23521 cgcgccccgc cctgccggac acttgcatca gctcggcaaa cagcctttcc ggcgcacgga
    23581 aatccgcgct gtacaggctg ccgtcggcgt tcaacacgat aacgaggttg agccgcgcga
    23641 aatcatggcc tttggcaagc atctgcgtgc cgaccagaat gtcgatttcg tcgttggcga
    23701 tgcggcggta caaatctgcc cagtcgtttt tgtgcgccgt actgtcccta tcgacgcgga
    23761 cgacggctgc cttgggcagc aaggcgcgca ggctttcttc gacgcgctgc gtaccgtgtc
    23821 cgacggcggt caggtcttgg ttgccgcagt cgggacattt gaacgggatg ggttcgcggt
    23881 ggtcgcagtg gtggcagcgc agttggcggg cgcgttggtg cagcaccatt ttggcggagc
    23941 agttcgggca gccgaaggta tggccgcagt cgccgcaaaa cagcgcaggc gcgaagccgc
    24001 gccggttaag gtacaccagc gacataccgc ccgcttcaaa gttctgtttc aaaagctgca
    24061 aggcttgcgg tgagaagccg ttatcgagtt tcagacgacc tatgttgagg atgtccactt
    24121 gtggcagttg cgcggaagca tgggcgcgtt cggtcagttg cagcaggcgg tacgcgccgc
    24181 tttgcgcctt gtgccaactc tccaagctgg gcgtggcact gcccaacacg acgggacagc
    24241 cgccctgctt cgcccgccac accgccaaat cgcgggcgtg gtagcgcaat tcgttgtctt
    24301 gtttgaacga gccgtcgtgt tcctcatcga ccacaatcag cccgacatca ggcaaaggcg
    24361 taaacaccgc cagccgcgtg ccgatcacca gcttcgcctg ccccaacatc gcgcgcagat
    24421 aatcctgcgt gcgcctgcct gccgccatcc ggctgtgcaa cacggcggtc ggcacgtcgg
    24481 caaaacggtt ttccacccgc tttaaaagct gcggcgtgag gttgatttcg ggcaacagaa
    24541 acaacacctg tcgcccctgc gccaacactt tcgccatcgc atcgaaatac acctcggtct
    24601 tgccgctgcc ggtaatgccg tacagcagaa acgaccggaa gcgtcccaat gccgtctgaa
    24661 tttcatcgga agccttctgc tggtcggtat ttaatacaaa ttcagagtgc gaagcctgcc
    24721 cgcggtacgg ccttaaaaca ggtttcgccg cttccgtcgt ttcaatccag ccctgctccg
    24781 cccaatcctc aatcaatttc gccgcctgcg cgtttgcctg tttcaacgcc gccatcgtca
    24841 tttcgcccga cagcagcgcg tcccacaaag ccgctttttt gttgaaccga gccggcggcg
    24901 gcgtttgcgc cctgcccgct tcgttcaaag cataaaacaa cggcggctgc ggcatttcca
    24961 ccgcgcgcgt ttccttcaaa ccctgcggca atgcggcaaa tacagcctgc ccaatcggat
    25021 agtggtaata acgcgacgta aacgccaaca aatcacgcca gctttgagac agcggctttt
    25081 cttccacaaa gaccgtctga acgctcaaaa tccgcgccgc atccatatcg ggcgcaatat
    25141 ccgtttccca caccatcccg acaacagttt tattgcgaaa aggcacaagc acccgcgttc
    25201 ccggaggaag cggctcggaa tgcgaataag tcaaaaggcc gtctgaaagc ggcacgttta
    25261 cagcgatgcg atggtagatc atacaggcag gcaaatcatt tttttgcgta ttttaacagc
    25321 cggaagctat tttcagacga taaattgagc ctcggcgcaa tgcaggcgca acagaggttt
    25381 gatacaatgc cacttttcca acacgctcaa tcatctttga tatgaaaacc aagttaatca
    25441 aaatcttgac cccctttacc gtcctgccgc tgctggcttg cgggcaaacg cccgtttcca
    25501 atgccaacgc cgaatccgcc gtcaaaaccg aatccgccgt caaagccgaa tccgccggca
    25561 aatccgttgc cgcttctttg aaagcgcgtt tggaaaaaac ctattccgcc caagatttga
    25621 aagtgttgag cgtcagcgaa acaccggtca aaggcattta cgaagtcgtc gtcagcggca
    25681 ggcagattat ctacaccgat gccgaaggcg gctatatgtt cgtcggcgaa ctcatcaaca
    25741 tcgacacgcg caaaaacctg accgaagaac gcgccgccga tttgaacaaa atcgacttcg
    25801 cctccctgcc tttggacaaa gccatcaaag aagtacgcgg caacggcaag ctgaaagtcg
    25861 ccgtcttctc cgaccccgat tgtccgttct gcaaacgctt ggaacatgag tttgaaaaaa
    25921 tgaccgacgt gacggtttac agctttatga tgcccattgc cggcctgcac ccagatgccg
    25981 cgcgcaaggc gcaaatctta tggtgtcagc ccgaccgtgc caaagcgtgg acggattgga
    26041 tgcgtaaagg caaattcccg gtcggcggca gcatctgcga caatcccgtc gcggaaacca
    26101 cttccttggg cgaacagttc ggcttcaacg gcacgccgac cctcgtcttc cccaacgggc
    26161 gcacccaaag cggctacagc ccgatgcccc aactggagga aatcatccgc aaaaatcaat
    26221 aatccatccg caaaacaaaa ggcttgaaac cgtgttccaa gcctttttca aatgccgtct
    26281 gaagcctgtc gcggcagctt cagacggcat ctttgttcca acctcaatcc tgcgccaatg
    26341 cgtctatcgg attgagtttg gctgctttat tggcaggcat aaagccgaac gcgatgccga
    26401 ttccggtcga acaggcgacc gcgccgatga cggatgccgc cgaaatgtcc atcgggaaat
    26461 cggttacaaa atgattgaac acgaggctga cggcggcgga caaacctacg ccgaccaagc
    26521 ctccgatgat gcagattaac accgcctcaa tcaaaaactg ctgcaaaata ttgccgcgcc
    26581 gcgcgccgat tgccatccgt atgccgattt ctttggtgcg ctcggtaacg gacaccagca
    26641 taatgttcat cacgccgatg ccgccgacta ccaatgaaat cagggcgatg gaggaaatca
    26701 gcagcttcat cgtaccggtg gtgctttcga ccatctgcct gatgctgtcg ctgttgttca
    26761 taaagaagtc ttccgtgccg tgccgtgctt tgagcagctc ggccagccct ttttcggcaa
    26821 cccgggtatt ggcattgtct ttgattttga cggtgatgga gttggtgtgg ctctcgcctg
    26881 tgatttggtg catcaccgtc gtatagggcg accaaagcat cagcacgtcg gaattgccga
    26941 aagcgttttc gtcttttttc atcacgccga tgacggtcaa ggggcgtttc ctgaacaaaa
    27001 tggttttacc caacggatcc gagtccgcaa agagtttgtc tttgacattt tggtcgatga
    27061 cgacgacttg cgcgtcttct ttcacatcgt tctcatcaaa cagccgcccc gtttccagct
    27121 tcagcccgcg cacgtcgaaa tattgttcgc ccacgccgta caaagaagcg gtcaggtcgg
    27181 tattgcggta ggtcagcgtg ccgccgctcg aagtcatggg cgtggcggaa gcaacgtagc
    27241 tttgtttggc gatgattttt gcgtcgtcta tggtcagggt tttgattttg ccgctgcgcc
    27301 tgtcgccgaa gccgcgcccg gggaagatgc tgatggtgtt cgtccccatc gaactgatgt
    27361 cttcgaggat ttttttctgc gaaccgttgc ccaatgcgac gacggagaca accgaagcga
    27421 taccgatgat gatgccgagc atggtcagaa gcgaacgcat tttgtgcgcc aatactgctt
    27481 gcaccgacat tctgaaggct tcgacaaact ggtcgtaata aaacgaccac gaggcttttt
    27541 cctgaatcct cccgacattg cttgcgggga tttcgggatt tttcgaggtg tcggaaatga
    27601 tttcgccgtc ccggatttcg atgacgcggt tggcgttggc ggcaatgccg gggtcgtgcg
    27661 tgaccataat gacggtatgc ccggcttcgt gcagcctgcg gatgatttcc atcacatttt
    27721 tgccgctggc ggtatcgagt gcgccggtcg gctcgtcggc gaagatgatt tctccgccgt
    27781 tcatcagggc gcgggcgatg gagacgcgct gctgctgtcc gcccgagagt tcgccgggct
    27841 tgttgccctc tttacttgcc aaacccaaat cctgcaagag tttgtccgcc cgcgcggaac
    27901 gctctttgcc gcccataccc atatacacgg ctggcagcgc gacgttgtcc cttgcggtca
    27961 gcgagcttaa gaggttgtag cgttggaaga tgaaaccgaa gcgttcgcgg cgcaatgccg
    28021 ccagttcgtc aggctgcatt ttggcagttt cgatgccgtc gattcggtac gaaccggaac
    28081 cggcggtatc caaacaaccg agtatgttca tcagcgtgga cttgcccgaa ccggactgcc
    28141 cgatgatggc gacaaaatca cccttctcta tcgacagact gatgtctttc aaaatatgga
    28201 cgcggttctc gccgctgccg aagcagcggt tgatgttttt acattcgatc aagctcataa
    28261 tattccgtaa atcaataaca aatgccgtct gaaaccgttt ccgtgttcag acggcatatt
    28321 cgtttatcgg cgcggcgggc cgcccatgac gcgttcgccg ctttcctgtt gctcggcggc
    28381 ggttatttcg gagatgacca ctttgtcccc ctctttcaaa ccgcttttca cttcggtatt
    28441 catactgtct ttcataccgg ttcggatttc gcgttccact gccttgccgt ccgcacccaa
    28501 cacgcgtacg aacgccttgc cgccgcgatt tttcacggtc agcgacggaa taagcaacac
    28561 atttttcaca ccgtcgattt caaccgtatt ctgcgtcgtc atccccgtgg cgagtttgcc
    28621 gtccggattc ggcacaaacg aacgggcata ataatagacc gcattggaag ccgtatccgt
    28681 actgctgttg tagccgcccg acgacatcgt ggtcagcccg gggtcgacgc tgtcgagctt
    28741 cgccttaatc ggcgtatccg gttcggacaa aatcgtaaac gaaatatcct gccccgcctt
    28801 caccttggta atatcgccct cggcaatctg cattttgttc aacatcatat ccagattcgc
    28861 caattggaca atcgtcggcg tagactgcgc cgcgttcaca gtctgcccct cttccacggg
    28921 aatcgccacc accgtgccgt ccatcgtcgc ggtaatgcgc gtgtagccca aatccgactc
    28981 ggcggtattg atggaaattt tgctctgtct gattaaagcc ttcaactcgg caacattggc
    29041 tttggcggcg gcaagcgcat cctgcgcgct ttccaaatct tctttagagg tcgcatcatc
    29101 cttccacaac gccgcctgac gcttatattt cttctccgcg ctgcccaatg caatctgtgc
    29161 cgacaccagc ttcgcctgat acgtttccaa tttggatttt tccatatcga gcgtgttggt
    29221 ctgcgtggtc gaattgattt ccgcaatcaa atcgcccttt ttgacctgtt gcccgagttt
    29281 gacataaagc tttttaatct gccccgaagc ctgcgcgccg accgatacca ggttggacgg
    29341 cgaaatctcg cccgtcgcgg aaaccgtccg gctgatgccg ccgcgcctga ccgtttccgt
    29401 aatataagaa gcctgcggct cgggcttcag ataatgccat ccgccccaaa ccgctgccgc
    29461 cgcgactgcc gcaacagccg accatttcat cattttcgcc atattttcaa ttctctgtat
    29521 gcgtcaatcc cataaaccgg aagcggattc taccgcaagt ccctgcccca tccaaggcgg
    29581 caaaccgccg acaataaaaa taaccatttg ttttaaatgg cttatacaat ctgctttccc
    29641 gaaaatttca atccgtttac atattccgcc atttccgata cggcgttata atgccgccca
    29701 caaacaacca aatggaaaca cattgctatg aaaaacgttc aaaaaggctt tacgctgctc
    29761 gagctgatga ttgccgtcgc catcctcggc atcctgacgc tcatcgccta cccttcctat
    29821 aaaacctaca tccggcgcgc ccgcctgtcg gaggtcaaat ccaccctgct ggtgaacgcg
    29881 caaaacttgg agcgttacta ccgccaaaaa gggacgtttg aaaaatacga ctcaaccaaa
    29941 ctgaaacaaa acaaatattt caagattacc ttaatcaaag ccagccccga ccacttcacc
    30001 cttcaggccg ctcccgatac ggcgaccaac gaaggcgaaa cctgcaccgt tacgctcaac
    30061 gacggcggta ccattgccgc ttccggtaca aaccaatcct gccccggctt cgactaatcc
    30121 tccgccgaag cccccaaacc cgtaaaaatg ccgtccggac acagcttcag acggcatttt
    30181 tacgggtttg cccctccgtt ttttcaaact tgaaacctgc cgcccccgca tccggccgcc
    30241 gtccaatgcc gcgcctcccc aactgttata atataacata tatttttcaa caacatacca
    30301 tttgaccctc aagatgcgga agcgtatgct tacccttata tcggacaaca ttccgtttac
    30361 aggtttttta ccttgaaaag gagcgcaatc atgaaaatgc aggcagttgt tgtgaataaa
    30421 aatgtagcgg gcgatgtgga agtagtcgaa cgcgaggttc gcccgttgga atacggcgag
    30481 gcgttggtcg aagtcgaata ttgcggcgtg tgccacaccg acctgcacgt tgcggcaggc
    30541 gactacggcg aaaaaccggg ccgcgtgctg ggacacgagg gcatcggttt ggttaaagaa
    30601 gttgccgacg gtgtgaaaaa tctgaaagtc ggcgaccgcg tcagcatcgc ctggctgttc
    30661 caaagctgcg gctcttgcga atactgcaat accggccgcg aaaccctgtg ccgttccgta
    30721 ttgaacgcgg gctacaccgc cgacggcggt atggcgaccc actgcatcgt gagtgccgat
    30781 tacgcggtca aagtccctga aggtttggat cctgcgcaag cttccagcat tacttgtgcc
    30841 ggcgtgacca cttataaagc cattaaagtt tccggcgttc gtcctggtca atggattgcg
    30901 atttacggcg cgggcggctt gggcaacttg ggtgtccaat acgcgaaaaa agtattcggc
    30961 acgcacgttg tcgccatcga catcaacgac gacaagctgg catttgccaa agaaaccggc
    31021 gcggatttgg ttgtcaacgc cgccaaagag gacgctgcca aagtgattca ggaaaaaacc
    31081 ggcggcgcac acgctgcggt cgtaaccgcc gtatctgctg ccgcattcaa ctctgccgtg
    31141 aattgcgtcc gcgcgggcgg acgtgtggtt gccgtcggac tgccgccgga atcgatggat
    31201 ttgtccatcc cgcgtttggt tttggacggc atcgaagtgg tcggctcttt ggtcggcacg
    31261 cgcaaagatt tggaagaagc cttccaattc ggcgcggaag gtttggttgt gccgaaagtc
    31321 caactgcgtg ctttggatga agcacccgcc attttccaag aaatgcgcga aggcaaaatc
    31381 accggccgta tggtgatcga tatgaaaaaa gaatgcggct gcggtcatca ccactgattt
    31441 gacgtggcgg tacacatcga aatgccgtct gaacgctgtt cagacggcat tttttatgga
    31501 tttggatttg attttaatct gttctgtttg aaataccgtc tgaaaaccct tatcgcaaca
    31561 cgtcattata gtggattaac aaaaatcagg acaaggcggc gagccgcaga cagtacaaat
    31621 agtacggcaa ggcgagccaa cgctgtaccg atttaaattt aattcactat accgtctgaa
    31681 aacccgtatc gcaacacttc attaaaaacg gcaagattca gccgttctgc aacccgttca
    31741 aacggcttcc gccatttcca acgcctgctt gatgtctacg gcgacgacgc gcgacacgcc
    31801 tttttcctgc atggttacgc cgaccagctg ctccgccatt tccatggtca ggcggttgtg
    31861 ggagatgtag aggaactggg tttgcaccga catttctttg accagcttgc agaaacgcga
    31921 ggtgttggca tcgtccagcg gggcatcgac ttcgtccaga aggcagaagg gggcggggtt
    31981 gaggctgaac agggcgaaca cgaggctcat ggcggtgagg gctttttcgc cgccggagag
    32041 gaggtggatg gtgctgtttt tcttgccggg cggacgcgcc atgatggaca cgccggcggt
    32101 caggaggtcg tcgcctatca ttttgagggt ggcttcgccg ccgccgaaca gggtcgggaa
    32161 gaaggtttgg actttgccgt tgacggcgtc gaaagtttct ttgaaacgcg ctttggtttt
    32221 gtcgtcgatt tgggcgatgg cttcttccaa aagcgcgatg gcggcctgca cgtcttcgct
    32281 ctggctgcgg tagtagccgt cgcgttcgcg cgcttcttcg agttcttgca gggcggcgag
    32341 gttgacggcg ccgagggctt cgatttgccg ggtaaggctg ccgatgctgc tgttcaatac
    32401 tttcgccgat tctttcgcca acgcttcgag cacgtccaga tcggcagcgc gttcggtcag
    32461 gttttgatgg tagcgtttgg cgttgatcag ggcctcctgc tgctgcaaca gggcggtttg
    32521 ggtggcggcc tgaagctgcg gcagcttggt ttgcagggtt tgtacgcggg cgtattgctc
    32581 cctgccctgt tcctgaatct gcgcgagttt ttcctgcacg gcgatgtatt cttcgtccag
    32641 cgtgtgtacg gtttcggtca actcgtcgag cttgatgtgc tgctcgtcgt tttggaactc
    32701 ggtttcatag gcgagggcaa gctcttgccg gcgttcctgc cagtccaggg tttgctgttc
    32761 gagccgggcg atttgctgcc ggtagttttg tttttgctgg ttgagtttgt ggacggcgac
    32821 ttcggcaagc ccgtattggc ggttggcttc caacagggca agctgcgcct gtttcagacg
    32881 gccttgctgc tcttggcggc tgtgcgcggt ggtttgctgc tggtgttcga gttcggcggc
    32941 ggcttcctgc aaagtagcga tgtcgtctga aagcccgtcg gacgtgtgtt gcaacacggt
    33001 ctgctcttcc gccaactgtg ccagttcgcg ctcgatgtgt tcgcggcgga tttgcccttg
    33061 gttggtgcgc gccagaagtt cggcggtgcg ttgctgcgct tggctgtatt ggcgcgtgtg
    33121 ttgctgttgc tgctgcatca gatttttgtg ctgcacttcg gacgagcgca cggcagcctc
    33181 cgcctgtttg aacgcggctt cggcggcgga aagtccgggg gcgaggtttt ccagttcgga
    33241 cgcgatgccg tcgaggcgcg ctttttgggc aatcaggctt tcctgcgcgg gcttggcata
    33301 gagcaggacg ctgactttat cgacctgatg gccttcgggt gtgagccaga tttgatgcgt
    33361 gcccaaatcg ctttgatgcg cgagggcgta gctcaaatcg ggcgcgcaca atacgccgtc
    33421 gagccagtgg tgcaatgccg tctgaaacgg cggctgcgct tggatttggt tcagcaatgc
    33481 ctgtacgggc agggattttt tgatgccgcc ggagaggccg tctgaaagcc atgccgcctg
    33541 cccctgcggc aaaggtgcgg gcggtacgaa actattcggc acgctacggg cgtgcaggcg
    33601 ttcggcaagg atgacggaca gggcgtgctg ccactcggcg ggcgcggtga tgtgttgcca
    33661 cagttgcggc gcggcggcgt ggtcggttgc ctgccaaaag tcggcggctt cctgctgttg
    33721 cgacaggatt tgcgacaacg cctgctgctg cgcctgcaag gtaatgtgtt gctgcttcag
    33781 gctttggaag cggtttgagg ccgtctgaaa cgcctcgcgg gcggcgtgca aggcttcttc
    33841 ggcggcaatg atttgttctt cgtaatgctc ttgccggctt tgcaacaagg cggcggcttc
    33901 ctgagcggcg gcggtttcgg cctcgtcggg caggttcagg gcttggtttt cctgcttcag
    33961 acggcctttg cgctcttcgt gcttggcaac ggtttgttcg gcatgggcaa gctgctgctg
    34021 cttcaacgcc agttcgcggc ggatgcggtt tgcctcgtcc tgctgggttt ggaaggcagc
    34081 gttgagcgtg gcttggactt cttccaattc gggcagacgc tcttcgtgtt cggcaacctg
    34141 catcgcccat tccgccaatt cggtttgctt ttcttcgacc tgcaactcgt tttcctcaag
    34201 ctgcacgcgg atttgctgct gctcctgccg gatgcgttgc aactgcgcct gcgccgcctg
    34261 cttgtcgcgt tcgatgcgtt ggtgcaggtt ttgccggtgg cggatttgtt cttccaaacg
    34321 ggcaatctgc tcgcgcaaca cgccgcgttt gttgctcaat tcgtgtactg cctgctgctg
    34381 cgactgttcg gcagtctgca aggcgtgtac ttcgttattt aacgcctgaa cctgtgcggc
    34441 ggtttcatcc tgctgcgcct gcaaagattg atgctgcgcg gtcgccttgt cggcggcggc
    34501 aagcgattgc tgccattggg cgtaatcgag caaatcctgt tgttgattca actgcgcggt
    34561 cagggatttg tagcgttcgg cggtttccgc ctgtttttcc agcttttcca cctgacgcgc
    34621 caactcgttc tgcaaatcgc ccaaacgctg caaatgctcg cgcgtgtctt tcagacggcc
    34681 ttccgtctcc ttgcggcgtt ccttatattt ggacacgccc gccgcctcct cgatataggc
    34741 gcgcaactcc tccggccgcg cttcgatgat gcgcgaaatc atcccctgct caatgacggc
    34801 ataaccgcgc gcgcccacgc ccgtacccaa gaacaaatcg gtaatatcgc ggcggcgcac
    34861 ggtctgattg ttgatgaaat aagtcgattc cccctgacgc gtcagctgcc gcttgatgct
    34921 cacctcggca tactgccccc acgcgccctg caaactgtgg tcgctgttgt caaataccag
    34981 ctccaccgaa gccctcggcg caggacggcg cgtcgccgca ccgttaaaaa tcacgtcctg
    35041 catactctcg ccgcgaagct gcttcgccga agcctcgccc aacacccagc gcaccgcgtc
    35101 aatcacattc gacttgccgc agccgttcgg cccgataacc gcgacaagct gccccggaac
    35161 atgaatcgtg gtcgggtcgg taaaagattt gaagccggag agtttgatgt gggtcaggcg
    35221 cataatggtc ggaaaaaata aaaaagaagc gtattttaac ggaaatcccg ccgcaccacc
    35281 ccatatcttg ccggcaaaac cttaccgcat cccgccccgg tgcggcagga ttttgcacat
    35341 cccggccatc cgtattaaaa tctttgcgtt tcccacactt tcctgacaaa tatacggagg
    35401 aggattccat gaaaacaaaa acagtcaagc aagcaagcaa gcaagcaagc aaaatagtct
    35461 aacccccctt tctttaatcg ctctacttaa tttccacttc tttttatcct gttcgatctt
    35521 ttgcggctgc acctcaagaa ataaactatt cgttatcagg cggtaaagtt tacgcagata
    35581 agttcagtat cgccattggg gttgaggcaa aagctcaggg ggataatacc aaacttagca
    35641 acctcaatcg gcacatctgc cgaagcaaat gcacccgggg cattggcttt aaggggaagt
    35701 tctgaagcat cgaaaaaatt tagtatagca gaaggctatc tggcatctag tgatggctat
    35761 ggtgcaatag cgattggttc tgccgccaag ataaagcaat tggaaaaagg cacaataaat
    35821 catattgtgg gaaacgataa taaaggtctt tatgtcgatg cagacggcaa tgtaacaaag
    35881 ataaccgtaa ggactgagtc ggaaaaagat attctctcaa gatacggtca aacttacggt
    35941 gcggtggcac tagggtttag atcttcttca cataatcttt ttgccagttc atttggagcg
    36001 ttttctacag ccacagctat tgaaagcttg gcagtcggcg acagcagcca atcaacgggc
    36061 taccgcagtg ctacttttgg cagtcacagc agggctttgg cagaagaaag tttggcatta
    36121 ggttatgaaa ctcgggcaaa tgcttatggt tccgttgctt taggtgcaga atctgtggcg
    36181 aatgaagaaa ataccgtatc agtgggttcc gatacattga aacggaaaat cgttaatgtc
    36241 gccgatggca cggaagattt atgatgcagt aaatgtccgc cagttgaacc gcttaagcaa
    36301 acgtacaaac cgcgtcggcg caagtgctgc ggcgttggct tcgttaaaac ctgcacaatt
    36361 aggcaaaaac gacaaattcg ccttttcttt gggctttgga agctataaaa atgcccaagc
    36421 ggtggcaatg ggggcggtgt ttaagcccgc tgaaaatgtg ttgcttaatg tggcgggcag
    36481 ttttgccgga ccgggctttc ggcgcggggg tttcttggaa attcggcggc aaaccgacac
    36541 ctgcggttgc cgcacaaaac gcggcgcatc ctgcaaaagt tttgcaattg cggcaggaag
    36601 tggcggcgtt gcgcgcccgg caggcagaaa ccgaccgcaa actgcacaaa caggccgaaa
    36661 tggaaaacga gctgcaacag ttgcgccgcg cgttgtccga attgaaaaaa cattgaagca
    36721 ataaacgtaa ggcgggacgg cggctctgcc tttttccgat gccgcacccg cgcgatgccg
    36781 tctgaagcct tcagacggca tttttaacgc gcccgaaccc cggttccgcc aatgcccgat
    36841 gcttggcgcg gcatttgccg tattgccccg ccgccgggcg cgttataatc cgccacgaca
    36901 tccgcccgat ttggaaacct gcctatgcga ccctcctatt tcatttccga cctgcatttg
    36961 agcgaaaagc accccgaact gaccgaactg ctgctgcgtt ttttacgttc tgccgccgcc
    37021 gggcaggcgc gggcggttta tattttgggc gatttgtttg atttttgggt gggcgatgac
    37081 gaagtttccg agttgaatac ttcggttgcg cgggaaatca ggaaattgtc cgacaaaggc
    37141 gttgccgtgt tctttgtcag gggcaaccgc gatttcctaa tcggtcggga tttttgccgg
    37201 caggcgggta tgacgctgct gccggattac tcggttttgg acttgttcgg cagcaacacc
    37261 ctgatttgcc acggcgacac attgtgcacc gacgacaagg catacctgcg tttccgccgc
    37321 atcgtgcatt gcaggcggct gcaaaaactg ttcctgatgc tgcccctgaa gtggcgcacg
    37381 cgccttgccg ccaaaatcag gcgtgtcagc aaaatggaaa aacaggtcaa gcccgccgat
    37441 attatggatg tcaatgccgc ctttaccgcg cggcaggttc gcgcctttaa cgcggaaagg
    37501 ctgatacacg gacacaccca ccgcgagcat atccatcacg aaaacggctt tacccgcatc
    37561 gttttgggcg actggcataa cgactatgct tcaatcctcc gcgtggacgg ggacggcgcg
    37621 gtattcgtgc cgccggaaga atgctgaaaa tgccgtctga agcctttcgg gcggcttttt
    37681 ctacacgccc ctttcccaca ctccctccgc cgcatcatca ccatccttaa cccgcattaa
    37741 tagccattat cgataaaatg cttgaccgat tacataagat tacgtaaagt gtgcaaacgc
    37801 ataataattg gtcttaccaa tttatctttt aaccaaaata tcgggtgtgc gggatttgaa
    37861 cgtctttcca aacctcgacg cgccttatga tgcaaatgcc taaaaaggat tcgttatggc
    37921 acttttcctc agcatattcc ccatcgtcct gctgatttgg ctgatggtga aaaaaaacag
    37981 tatgccttcc tacgtcgcac tgccgattac cgcagtgctg atttacgcca tcaaactttt
    38041 ctacttcggc gatgcgggaa tgctgctcaa cgccaccgcc gcttccggcc tcgtcaaaac
    38101 gctcacgccg attaccgtga ttttcggcgc gattatgttc aaccgtatga tggaaaccac
    38161 gggctgcatc gatgtcatcc gcaaatggct ggcgaccatc agcccgaacc ccgtagcgca
    38221 actgatgatt atcggctggg cttttgcctt tatgattgaa ggcgcatccg gcttcggcac
    38281 gcctgccgcg attgccgcgc cgattctgat gagcttgggc ttcaatccgt tgaaagtggc
    38341 gattttcact ttggtgatga attccgtgcc ggtatcgttc ggcgcggtcg gtacgccgac
    38401 ctggttcggt ttcgcaccgc tgaacctgag tgccgaagac atcctcgcca tcggcaggca
    38461 gaccggcgtg atgcacttct tcgcaggttt cgtcatccct gtcatcggtt tgggcttcat
    38521 cgtgccttgg tctgaaatcc gcaaaaactt gggcttcgtc gccattgccg tcttctcctg
    38581 caccattcct tatgccgcat tggcgatggt caacgaagaa ttcccgtcgc tcgtcgccgg
    38641 cgcggtcggc ctgatggtgt ccgtattcgc cgccaaccaa ggctggggct tgagcaaaga
    38701 ccacgccaaa gacccgaatg ccgaaaaagt gccgttcgcc caagtcgcca aagcactcgc
    38761 ccctttgggt atgctgatcg gcatgctggt ggttacccgc atcaaacagc tcggaatcaa
    38821 aggcattttg accagcaaag aagaatggtt cagcttccaa ctgccgtttg atttgtccaa
    38881 aattaccgtc agcgactccc tgacgattac cttcggcaat attttcggac aagatgtcag
    38941 cgcgtcttac caaacgctgt acgtcccggc ttggattccg ttcgtgctga ccgtttggat
    39001 ttgcatcctg ctgtataaaa ccaaattcaa agatgcctgg acgatttatg ccgtaacctt
    39061 caatcaaacc aaaaaaccgc tgcttgccct gatgggtgcg ctgattatgg ttcagctgat
    39121 gctggtcggc ggcgacaatt cgatggtgaa aatcatcggt aaggaatttg ccgcaatggc
    39181 gggcgaacac tgggtttatt tttcgccgta tctgggcgcg atcggtgcgt tcttctccgg
    39241 ctccaacacc gtgtccaacc tgaccttcgg tccgattcag cagcaaatcg cccttgatac
    39301 cggcctgtcc gtgaccctga ttctggcgtt gcagtccgtc ggcggcgcga tgggcaatat
    39361 ggtgtgcctc aacaacatca tcgccgtatg taccgtattg gacgtgaaaa attccgaagg
    39421 tgcgattatc aagaaaaccg ttatcccgat ggcgatttac ggcgtgattg ccgtcgtcgc
    39481 ggcaatgatt ttcttcctct aggcgaaacg ccgccatcca tagcggcagc acgcggggaa
    39541 cggaaaccgc cgctcgtccg ggcggcgcgt cgggcagaat cccggtgccg gaacagcccg
    39601 ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc agacggcatt tttatcgccg
    39661 tttgcattca ggcgtagtaa tgatgcggca tcttgccgtc aaggcggtag cgcgtgccgc
    39721 aatacgggca ggcaacgctg cccgattctc cttcgcccaa aggtaaaaac accctcggat
    39781 gcccgttcca ctgctcgttg tccggtcccg agcaatacag cggcagattt tccggcaaca
    39841 cggaaatttc ctgcggattc agattgtcca tttgattttc ctttgcgtgg ttggcgtgcg
    39901 cccattttac gccatcggca ggctaaagga tattttcggc gcaaagccgc aatccgctat
    39961 aatccccact tttcagacgg cataccatga ctgcgcttac ccttcctgaa gacatccgcc
    40021 aacaagaacc atccgttctg ctctataccc tcgtttccgc ctacctcgaa cacaccgccc
    40081 aaaccggcga cgaatccctc tcctgcctgt ccgacgacca gcacacgctg accgcattct
    40141 gctacctcga cagccaagtc gaagaaggcg gcttcgtaca gcttatcgcc tccggctacg
    40201 gcgaatacat tttccgcaac ccgcttgccg acagcctgcg ccgctggaaa atcaaagccg
    40261 tgccgaaagt cttggacaaa gccaaagccc tctacgaaca acacggcgaa accatcgaaa
    40321 cgctcgccga cggaggcgca gacatcgaat ccctgcgcaa acagttcccc gactttgaag
    40381 aatgggacgg cgcatactac gaagccgccg aacaagacct gcccctgctt gcagaacaca
    40441 tcctgtcaaa ccgggcggca ttcgcccata tcgggcaggc gtgattgcgt ctgtttccag
    40501 cccgtgtaaa acagcgtaaa atcggcaaac ccgaattatc ttccgtcccc atcaaggagc
    40561 aagctatgtt cttcaagcac atcgaagccg cccccgccga tccgattctc ggtttgggcg
    40621 aagcgttcaa agccgaaacc cgccccgaaa aagtcaacct cggcatcggc gtgtacaaag
    40681 acgcatccgg cgcgacaccc attgtcaaag ccgtcaaaga ggctgaaaaa cgcctgttgg
    40741 aaagcgaaac caccaaaaat tacctgacca tcgacggcgt tgccgactac aacgagcaaa
    40801 cccaaatcct gctgttcggc aaagaccacg aaatcatcgc cagccgccgc gccaaaacag
    40861 cgcaaagcct tggcggtaca ggcgcattgc gtattgcggc cgaatttgcc aaacgccagt
    40921 tgaacgcgca aaccatctgg atttccaatc cgacctggcc aaaccacaac gccattgcca
    40981 aagcggtcgg tatccaagac aaaccttatc gctactatga tgccgccaaa cactgcttgg
    41041 attgggacgg catgattgaa gatttgaacc aagcgcaaaa aggcgacatc gtcctgctgc
    41101 acggctgctg ccacaaccct accggtatcg accctacgcc cgaacaatgg gaaactttag
    41161 caaaactttc tgccgaaaaa ggctggctgc cgctgtttga ctttgcctac caaggctttg
    41221 gcaacggttt ggaagaagat gcctatggct tgcgcgtgtt cttgaaacac aatacagaat
    41281 tgctgattgc cagctcttat tccaaaaact tcggcatgta caacgagcgc gtcggcgcgt
    41341 tcactttggt ggccgaagat gaagaaacag cagcccgcgc ccacagccaa gtcaaaacca
    41401 tcatccgtac cttgtattcc aacccggctt cacatggtgc gaacaccatt gcactggtgt
    41461 tgaaaaatga tgatttgaaa gcacaatgga ttgccgaact cgatgaaatg cgcggccgca
    41521 tcaaagccat gcgccaaaaa tttgtcgagt tgctcaaggc caaaggttca acccaagact
    41581 ttgattttat tattgaacaa aacggtatgt tctctttcag cggcttgact cccgaacaag
    41641 ttgaccgttt gaaaaacgag tttgccattt atgccgtccg ttccggccgc atcaacgtcg
    41701 ccggcattac cgacaacaac atcgattatc tgtgtgaaag catcgtgaaa
//
LOCUS       DAXDZG010000005        40873 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXDZG010000005 DAXDZG010000000
VERSION     DAXDZG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40873
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.005"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..75
                     /locus_tag="ACFB78_000233"
     tRNA            1..75
                     /locus_tag="ACFB78_000233"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Glu,seq:ttc)
     gene            374..523
                     /locus_tag="ACFB78_000234"
     CDS             374..523
                     /locus_tag="ACFB78_000234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268322.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
     gene            complement(520..2139)
                     /gene="mnmC"
                     /locus_tag="ACFB78_000235"
     CDS             complement(520..2139)
                     /gene="mnmC"
                     /locus_tag="ACFB78_000235"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC1268323.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            2259..2627
                     /locus_tag="ACFB78_000236"
     CDS             2259..2627
                     /locus_tag="ACFB78_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC1268324.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            2670..3407
                     /gene="ubiE"
                     /locus_tag="ACFB78_000237"
     CDS             2670..3407
                     /gene="ubiE"
                     /locus_tag="ACFB78_000237"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC1268325.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            3627..4106
                     /locus_tag="ACFB78_000238"
     CDS             3627..4106
                     /locus_tag="ACFB78_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC1268326.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(4234..4728)
                     /gene="folK"
                     /locus_tag="ACFB78_000239"
     CDS             complement(4234..4728)
                     /gene="folK"
                     /locus_tag="ACFB78_000239"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC1268327.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(4738..5112)
                     /locus_tag="ACFB78_000240"
     CDS             complement(4738..5112)
                     /locus_tag="ACFB78_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC1268328.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            complement(5166..5732)
                     /locus_tag="ACFB78_000241"
                     /pseudo
     CDS             complement(5166..5732)
                     /locus_tag="ACFB78_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(5817..6110)
                     /gene="hfq"
                     /locus_tag="ACFB78_000242"
     CDS             complement(5817..6110)
                     /gene="hfq"
                     /locus_tag="ACFB78_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC1268329.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            complement(6239..7177)
                     /locus_tag="ACFB78_000243"
     CDS             complement(6239..7177)
                     /locus_tag="ACFB78_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC1268330.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            7497..7937
                     /locus_tag="ACFB78_000244"
     CDS             7497..7937
                     /locus_tag="ACFB78_000244"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC1268331.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            7989..8864
                     /gene="xerD"
                     /locus_tag="ACFB78_000245"
     CDS             7989..8864
                     /gene="xerD"
                     /locus_tag="ACFB78_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC1268332.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRGSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            9036..9236
                     /locus_tag="ACFB78_000246"
     CDS             9036..9236
                     /locus_tag="ACFB78_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFC1268333.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEF
                     AASFLTAHNAQPTVTAGINVQA"
     gene            9250..9384
                     /locus_tag="ACFB78_000247"
     CDS             9250..9384
                     /locus_tag="ACFB78_000247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268334.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            9402..9635
                     /locus_tag="ACFB78_000248"
     CDS             9402..9635
                     /locus_tag="ACFB78_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC1268335.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(10007..10870)
                     /gene="rfbD"
                     /locus_tag="ACFB78_000249"
     CDS             complement(10007..10870)
                     /gene="rfbD"
                     /locus_tag="ACFB78_000249"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC1268336.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPHQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            11063..11926
                     /locus_tag="ACFB78_000250"
     CDS             11063..11926
                     /locus_tag="ACFB78_000250"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC1268337.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            12160..14283
                     /gene="pnp"
                     /locus_tag="ACFB78_000251"
     CDS             12160..14283
                     /gene="pnp"
                     /locus_tag="ACFB78_000251"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC1268338.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(14356..15024)
                     /locus_tag="ACFB78_000252"
     CDS             complement(14356..15024)
                     /locus_tag="ACFB78_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC1268339.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(15021..15335)
                     /locus_tag="ACFB78_000253"
     CDS             complement(15021..15335)
                     /locus_tag="ACFB78_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268340.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            15472..16323
                     /gene="dapF"
                     /locus_tag="ACFB78_000254"
     CDS             15472..16323
                     /gene="dapF"
                     /locus_tag="ACFB78_000254"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC1268341.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            16320..16925
                     /locus_tag="ACFB78_000255"
     CDS             16320..16925
                     /locus_tag="ACFB78_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268342.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            17121..18053
                     /gene="cysK"
                     /locus_tag="ACFB78_000256"
     CDS             17121..18053
                     /gene="cysK"
                     /locus_tag="ACFB78_000256"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC1268343.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            18362..19270
                     /locus_tag="ACFB78_000257"
                     /pseudo
     CDS             18362..19270
                     /locus_tag="ACFB78_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            19316..19465
                     /locus_tag="ACFB78_000258"
     CDS             19316..19465
                     /locus_tag="ACFB78_000258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268344.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            19689..19796
                     /locus_tag="ACFB78_000259"
                     /pseudo
     CDS             19689..19796
                     /locus_tag="ACFB78_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19901..20920)
                     /gene="lepB"
                     /locus_tag="ACFB78_000260"
     CDS             complement(19901..20920)
                     /gene="lepB"
                     /locus_tag="ACFB78_000260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC1268345.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(21066..22859)
                     /gene="lepA"
                     /locus_tag="ACFB78_000261"
     CDS             complement(21066..22859)
                     /gene="lepA"
                     /locus_tag="ACFB78_000261"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC1268346.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(23001..23702)
                     /locus_tag="ACFB78_000262"
     CDS             complement(23001..23702)
                     /locus_tag="ACFB78_000262"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC1268347.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RIVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            23870..25000
                     /locus_tag="ACFB78_000263"
     CDS             23870..25000
                     /locus_tag="ACFB78_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC1268348.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            25034..26011
                     /locus_tag="ACFB78_000264"
     CDS             25034..26011
                     /locus_tag="ACFB78_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC1268349.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            26015..26365
                     /locus_tag="ACFB78_000265"
     CDS             26015..26365
                     /locus_tag="ACFB78_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC1268350.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            26370..27149
                     /locus_tag="ACFB78_000266"
     CDS             26370..27149
                     /locus_tag="ACFB78_000266"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC1268351.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            27175..27390
                     /locus_tag="ACFB78_000267"
     CDS             27175..27390
                     /locus_tag="ACFB78_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC1268352.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            27491..27802
                     /gene="grxD"
                     /locus_tag="ACFB78_000268"
     CDS             27491..27802
                     /gene="grxD"
                     /locus_tag="ACFB78_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC1268353.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            27908..28534
                     /gene="upp"
                     /locus_tag="ACFB78_000269"
     CDS             27908..28534
                     /gene="upp"
                     /locus_tag="ACFB78_000269"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC1268354.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            28557..28877
                     /locus_tag="ACFB78_000270"
     CDS             28557..28877
                     /locus_tag="ACFB78_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC1268355.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            29046..29687
                     /locus_tag="ACFB78_000271"
                     /pseudo
     CDS             29046..29687
                     /locus_tag="ACFB78_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            29717..30541
                     /locus_tag="ACFB78_000272"
     CDS             29717..30541
                     /locus_tag="ACFB78_000272"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC1268356.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            30541..31509
                     /locus_tag="ACFB78_000273"
     CDS             30541..31509
                     /locus_tag="ACFB78_000273"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1268357.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            31791..32213
                     /locus_tag="ACFB78_000274"
     CDS             31791..32213
                     /locus_tag="ACFB78_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC1268358.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            32231..32995
                     /locus_tag="ACFB78_000275"
     CDS             32231..32995
                     /locus_tag="ACFB78_000275"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC1268359.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            33007..34341
                     /locus_tag="ACFB78_000276"
     CDS             33007..34341
                     /locus_tag="ACFB78_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC1268360.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            34338..35555
                     /locus_tag="ACFB78_000277"
     CDS             34338..35555
                     /locus_tag="ACFB78_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC1268361.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            35630..36694
                     /gene="hemE"
                     /locus_tag="ACFB78_000278"
     CDS             35630..36694
                     /gene="hemE"
                     /locus_tag="ACFB78_000278"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC1268362.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(36757..38631)
                     /locus_tag="ACFB78_000279"
     CDS             complement(36757..38631)
                     /locus_tag="ACFB78_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC1268363.1"
                     /translation="MNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLGNVQSG
                     KTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEADDRSFM
                     EVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTNSDKPA
                     KDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGEKYIGG
                     NFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKANCNFA
                     LHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIHHFDEI
                     YEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTVYYSRT
                     AKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKNSGGNI
                     QVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKDEIQSD
                     LYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRA
                     TGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK
                     "
     gene            complement(38647..39684)
                     /locus_tag="ACFB78_000280"
     CDS             complement(38647..39684)
                     /locus_tag="ACFB78_000280"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC1268364.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            complement(39681..40805)
                     /locus_tag="ACFB78_000281"
     CDS             complement(39681..40805)
                     /locus_tag="ACFB78_000281"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1268365.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
BASE COUNT        10087 a         9398 c        10989 g        10399 t
ORIGIN      
        1 gcgcccatcg tctagcggtt aggacatcgc cctttcacgg cggtaaccgg ggttcgattc
       61 cccgtgggcg tgccaattca aaatgcctcc gattatatcg gaggcatttc tcatttctca
      121 tttctcattt ctcatttctc atttctcatt tctcatttct catttctcat actattgagt
      181 cctcgagaag ggaaataaaa attaacatcc ttatatattg agttcctgag aagggaagat
      241 taacaaaaat taacgccctt tgcttcatac aagtaacagg gctttttcat cccttcctta
      301 tctaacaggg ggtacagaaa ccgaagcggc tggcagggtt aaggaagtct tcgaatgtta
      361 cggaaaattc atcttggaca gcaaaggcaa tttgttaggc attccttact ccttattttg
      421 ggaagaaaac gttatgggtg ttttcgatat tttaccgtca ggattggtat gtttatttga
      481 atatgatttt ttgtggtcgg gacggcatgc ggcaaaggct taaggaatca ggttctgcct
      541 tctgacgatg gcgcggatga cggtgcggtt ggggtgtagg gcgtggcgca ggcgttgtga
      601 aaagagatgg ggcaagccta ggatttcggc ggcaacggcg gcggcgcaga tgggggcggt
      661 ggcaagcccg cgtgtgccgt gggcggtatt ggtgtaggca ttgggcagat atgggcatgt
      721 ggtgtcgatg cggtagtttt tgtccagtgc aagtttggcg taagtctgcc gcatggcggc
      781 aatgtcgccg agtgcgccga cgacggggag gtggtcgggg ctgtcgcagc gtatggcggc
      841 gtgcccttgg tgtttttggg ggtttgggtt ggcggcaaac aatgattcgg caagggcggg
      901 gttaaggtgt gccaatgctt ggcggtttga ggcttcttcg gcttcgttcc atccggtatt
      961 gctgctgttg ggaataaaac tcgcgccgta gcagtgcagt ccgtgccacg acgggctgat
     1021 gtagctttcg cctgaaacgg cgcaacgcag ttgttcggaa aacggggtgg acggtgtgag
     1081 gccggtttgt ccgcgtattt gcctgagggg tagggcggcg aggttggttt cgggcaggca
     1141 ggggctgtgc gcgccggtgc agtagatgat gtgtgtggcg gtaaatgtgc cgtttggcgt
     1201 gcttgcaatc cacttttctc cgtcgtggga aatgccggtt aacgttgtgt tttcatatag
     1261 ttcgatcagc ggatggctga ggagggtgcg gacgaatgcg ggcggattga gccatacgcc
     1321 gtgttgccag tagagtccgc ataatggttc ggcgtagggc gtgttcagcg ggatgccggc
     1381 gattttttcg gcttctgcag acgtgatgct gcggtagagg tggttatggt gtttttgcaa
     1441 acccaattcg tgattgcgtt gttgttcggt gcggctgtaa ttgaggtgga tgatgccgtt
     1501 gccgccccaa gtgtcggagt cgggcaggat gtgtccgagc aggcgtttgg tgtagccgta
     1561 gccggcaagc agcagttcgg tctgtccggt gtcgtgcggc gagattttgg cgtaaagcag
     1621 cccttgccgg ttgccgctgg cggcttgagc ggcttttcgg gcttccaata cggtaacgga
     1681 aatgccgtgt gatgctaagg cgtgggcggt cgatgcgccg gcaatgcctg caccgatgac
     1741 gaggacgtgt tccggttttt tccgttcgga tgtttgtgga agtgcaaacc agggtttgtc
     1801 ggacttgctt tcggtttgcg ggatggcttc ggtctgccag gaaatgtggt ggaagtgttc
     1861 gtgcaggcgg cgggtgcgtg aagggggaac cagccagaag cggacatcgg gcaggatgtg
     1921 ttcgatgagg ttgatgctgt cgaactggag gcactgggtt gcctgatcca aacggtgctt
     1981 cagacggcat tccgagtccg aagcatcttg tgcggtttga aaatcgggaa tctgattatc
     2041 ggggaggcag acaatcaggt tgagcgggtg tgcgtgtttg cggatggttt ggtcgagtgt
     2101 gcggatgtcg ggaatgccgt cccatacgag attgtccata tcaatgccgt ctgaagtgtg
     2161 ggtttgaata tcggtatcgg gataaagctg ttaaaatacg cgccgtttaa aggcacgcgt
     2221 acgcctgccg gatattgtat gccgaaccga ggtgtttttt gaataatatt cctgttgaaa
     2281 tccgtttgtt gaaaaaccgt gccgtgttgg ttttgactta tggggacgaa cctaaaaatc
     2341 tgcctgccga atttttacgc gtctattcgc cgagtgcgga agtgcgcgga cacggcgtgg
     2401 gacaggatgt tttgcagacc ggtaaggcgg atgtgcagat tacggatttg cagcctgtcg
     2461 gacagtatgc gctgaaaatc agtttttcgg acgggcacga cagcggtctt tacgattggg
     2521 cgtatctgca cagactggca tacggatacg atgcgatgtg gcaggaatat ttggacaaat
     2581 tggcggcggc gggcgcgtcg cgttttgaaa agcaatcaga ttgttaagtt tgggatgccg
     2641 tagggcggta tcagggaggt ggttagaata tgggcggaca aaaaacgcat ttcgggttca
     2701 gcacggtcaa cgaagatgag aaagccggaa aagtggcgga agtgtttcac tccgtcgcca
     2761 aaaactacga cattatgaat gatgtgatgt cggcagggct gcaccgcgta tggaagcatt
     2821 ttaccatcca tacggcgcac ctgaaaaaag gcgataaagt gttggacatt gcgggcggta
     2881 cgggcgattt gtcgcgcggt tgggcgaaac gggtcggcaa ggaaggcgag gtttggctga
     2941 ccgatattaa ttcttctatg ctgaccgtcg ggcgcgaccg tctgttgaac gaaggcatga
     3001 ttttacccgt gtcgcttgcc gatgcggaaa aactgccttt ccccgacaat tatttcaact
     3061 tggtctccgt ggcgttcggc ttgcggaaca tgacgtataa agatgccgcg cttaaggaaa
     3121 tgtaccgtgt tttgaaaccg ggcggtacgt tgctggtgtt ggagttttcc aaaatctaca
     3181 agccgctcga aggcgcatac gatttttatt ctttcaagct gttgccggtg atgggcaggc
     3241 tgattgccaa agatgcggac agctaccagt atctcgccga atccatccgt atgcaccccg
     3301 atcaggaaac tttgaaacag atgatgctgg atgcaggctt cgacagcgtg gattatcaca
     3361 atatgagtgc gggcatcgtc gcgctgcata agggcgtgaa attttaaacg gactgcctgt
     3421 gaagccaatg ccgtctgaac acgtttcaga cggcattttt gtattttttg agacaaaggt
     3481 tttaaatctt aaaaattaat tcatatattt atcaataatt tataaacttt ttaaaaaata
     3541 ggaacaatta tcatttgtaa aattgggaga tgtctgtata atgcagtcaa tccagtaaac
     3601 aacgcagcag acgaaaggag ggaaaaatgc cggaaagtat tttcaaacag atttcctccg
     3661 atattttgag gctgcaccgt gattctgttt attccctgct agcaacctcg ggttgcaact
     3721 gtcaggtgca tgaagcggca tatgtcaata ttgacggcaa atattatatt gcgctttcgt
     3781 gcgaacccga ggtgggggaa gttgaaacag gtattttgtt gattgaagat gaaagccgca
     3841 accttcgttt gagctgggtc ggtagtgcga gggagctcga ccgcaaggat aatgcctaca
     3901 aacgcgccct gtccgcgttg tccagaaagc tggggcggtg taaggacaag ctgcatacgg
     3961 cggttcagcc gtttttgttg gagctggtgc cggagaaagg cagattttct gtcggtgatg
     4021 aagaagtttg gatttctcga aacgatttag tgagggcctt atatcctgtc gggtacagta
     4081 tgcggcaggc agtgttgcag atttaaagtt ttggtagtgg tttgtgttcc ttttgcgccc
     4141 ctttctcaag gggcgatttt ttttgcacgc gtgttggcgg caaaggaaaa atgccgtctg
     4201 aaaccggtgt tcggacggca tccgcgtgcg gaattacctg tccggtaaaa gacggatgcc
     4261 ttgattgccc agccgttttg acaattcgac aacctttccg tattttccca aaataaaatc
     4321 agggaggatt tctgccaaag ggcgtatgac gaaactgcgt tcgtgcgcgc gcggatgcgg
     4381 cagggtaagg cgggggtcgt cgctggagat gccgtcaaag tcgataatgt ccaaatccaa
     4441 tgtgcgcggt gcattgcgga aactgcgttc gcgtccgaaa tcggcttcga tacggttgag
     4501 ttcggcaagc agggcaatgc cgtccaaggt ggtggaaacg gtgcagacgg cattgatgaa
     4561 atcgggctga ttgtcgtaac cgacgggtgc ggtcatatac agtgaggaag cctgttcaag
     4621 ccggatgtca ggatgggacg agagcgcgtc taatgcgccg cgtatttgtt gtgcggggtt
     4681 gtcaaggttg ctgcccaagg cgatgacggc aaaatgtctg ttgttcatgg cagtgtttca
     4741 gaatggcagg actttggttt tggcaaggta aacgatgcag gcgatgcaac acatagcgag
     4801 caggtaaacg gtgtagaact tggtcgaacg cggacgggcg cgcatcatta ccatgcccag
     4861 tgcgatgtag gcgaacagga gcaggatttt tgtgccgagc caaggcgcgt tgaacgggga
     4921 gaaatgggta atcttcatca gccacaatcc cgtaaacagc agcatcgtgt cgttgaggtg
     4981 gggcagtgct ttccaaaagc cgaccaaggg cttttctgga tttttccaaa gtaggaaaaa
     5041 acggatgttg aataccaaaa tggtgatggt aacgaagatt tgatggctgt atttgacaat
     5101 cagatactgc atggtcggct cgtatcaaaa taagggttag aatcggctta ttttaccgta
     5161 aacagttatt tttgacgcag tttttcaaat gccaaaagat agggtgggct attcttccgg
     5221 ttggtaaagc cgtaatgtaa aacggcaaac tgttcttgag gcaggttttt tgcccattgt
     5281 ccgacggctt ctgcctcctg tttgccgttt tcgtgtcccg gatagaggac ggcaataagc
     5341 ataccgtctt ctttcagcag ggagagggtt gctcaaaggg cgggaatgct ggtttccttg
     5401 tcggtggtca gattcttgtc gcctccgggc aaccagccga aattgaaaac ggctgcatcc
     5461 agcggctccg gaatatattg cttcaggttt tcatgtccgt ccaagatgag ccgtacattg
     5521 ctgtaacctg cttcctgcag acggcatcgg gtgttgttca gggcttgcgg ctggatgtcg
     5581 aatgcccaca ctttccccct gttgcctgcg gtttgtgcga ggaaaagggt gtcgtgtccg
     5641 ttgccggcgg tgccgtccag cgcattgccg ccttcgggaa gtgcctcccg caaaaggcaa
     5701 tgggcgaatg gaaggatgtt tttcaataat atttttaaat gccgcctgaa aataaaaata
     5761 ccttaccgtt gtccggtaag gtattgaaag atatgacacg tcatgcttcg tgcggattat
     5821 tcggcaggct gctggacggt ttccacttgg acaagggtcg aagccggtgc ggcttggggt
     5881 ttgttttcat gttggaggtt gacggagcga gccggtacga tggtggaaat ggcgtgtttg
     5941 taaaccattt gggttacgga agtgtttctc agaagaacga cgtattggtc gaaagattcg
     6001 acctgacctt gcaatttgat accgttaact aagtaaatcg aaaccggaac atgctcttta
     6061 cgcaatgcgt tcaggaaggg atcttgcaac atttgtcctt tagctgtcat attttttaac
     6121 tccgctatta tgattgtgaa atcgggcaga tgccctgtgc gtgtcgaatt gtagcctgga
     6181 ataggaaaaa ttaccaattt ttttgtgttt gggcggtttt ccgccgggca tttgtatgtc
     6241 aggagcgttg ctgcagcatc cacgattcga ttttgcgggc gtcgttgacg cgtgtggcgg
     6301 atgtgggcga gttcagcaat acgatggtaa cgggttggtt ttgaatgttg gctttgacaa
     6361 ccatagacct gcctgcctcg cggatgtagc cggttttctg caattcgatg ttccacatgc
     6421 cttccctgac cagggcattg gagtttttat agttttgctg cccgtttttg gtttgtaccg
     6481 aggcgtagtt ggaagtcgag ttggtgcgga tttgcggata ttgggcggcg gcgttgacca
     6541 tgaggctcag gtctttggcg gtggaaacgt tttggaagtt gagtccggtc ggttcgtaaa
     6601 agcggctgcc gtacatgccc aggctttggg ctttgcggtt catggcggcg acaaatgcgc
     6661 ccatgccgcc ggggtaggtt ctgcccaatg catgggtggc gcggttttcg ctgctcatca
     6721 ggctaaggtg cagcagttct ttacgtgtaa gtgccgtacc tatggcaaga cggctgccgg
     6781 tccctttgag gcggtcgatt tcgtcgggtg tgatggtaac ggtttcgttc atgtccaagt
     6841 ttgcatccaa aacgaccatc gcgctcatga gcttggaaat ggaggcaatg ggcataatcc
     6901 tgtcggcgtt tttctgatac agtatttgcc ctgttttgtt gttgacgacc agtgcggatt
     6961 gggaggacag aatcagcccg cccgtaatgg cctgggtatc ggcgggatat atcgtgcttt
     7021 cggcgaatat ttctatcgga tcggaggagg taagcatgtt ctgttctaaa aattgcccta
     7081 aaatgtcgtt gtcggcaaaa aggtgggctg acggcaggga aaggcagagg ccgagcagca
     7141 gcgatgaggg caggcgtttc agggtgcgga tggacatttt tgattccgta tttttgagta
     7201 ttggcgttat tttgttgaaa aaacagccat ctgtaaagta tgtcgtctga caggagactg
     7261 cccgtaggcg ggcggtcttg ttgtgttttt ggagttgggt tttataacat tctgttttta
     7321 aatcggaaca tattttgtgg tttgacatgg atatttttca tgccgtcgtg tgtcggtttg
     7381 gatgtttccg gcggttgaat ccttgtcctt ggggcggtag aatcggggtt ggtttggcaa
     7441 ttgcggcggt gcgtctgcgt gccgttttga ataatgggaa tatcgggagt aggactatgg
     7501 atatgaaata tgaatttacc ctgccttcga gcagcggtgc ggattttcat tcggcagaac
     7561 atctgccttt ggtcgtgtat ttttatccga aagacagtac gccgggctgt acgacggaag
     7621 gcttggattt taatgcgcgt ttggaacagt ttgaggcatt gggttatacc gtggtcggta
     7681 tttcccgcga cggcgtaaag gcgcatcaga atttttgcgc caagcagggt ttccggttcg
     7741 agctgttgag cgacaaggat gaaacggtgt gccgcctgtt tgatgtcatc aaattgaaaa
     7801 aactgtatgg gaaagagtcg ttaggcatcg agcgcagtac gttcgttctg aacaaggacg
     7861 gcgaaattgt ccgggagtgg cggaaagtca aagtggcggg tcatgcacag gaagtgttgg
     7921 aaacgctttc ccgatgatgt ggaccatgcc gtctgaagaa gattcagacg gcattggttt
     7981 ggaacgctat ggaaaacggt ttgattgaca ggctgctgga aactttgtgg ttggatcggc
     8041 ggctcagtcg gaatactttg gacagctacc ggcgggattt ggaaaagatc gcccgccggc
     8101 tgtctttgtg cggcagaacg ctgaaggatg cggacgaagc agatttggcg gcggcggttt
     8161 atgttgacgg ggagcaacgg ggttcgcagg cacgcgcact gtcggcgtgc aaacgcctgt
     8221 atgtgtggat ggaacgggaa gggatgaggg cggacaatcc cacccgcctg ctgaaaccgc
     8281 caaaaatcga cagaaacatc ccgacactga ttacagaaca gcagatttcc cgactgcttg
     8341 ccgcaccgga taccgacacg ccgcacggtt tgcgggataa ggccctgctc gaattgatgt
     8401 acgcgaccgg cttgcgcgtc agcgaggcgg tcgggctgaa tttcggcaat gtggatttgg
     8461 acaggggttg tattaccacg ctgggcaagg gcgacaagca gaggatggtc ccgatgggac
     8521 aggagtcagc gtattgggtg gaacgctatt atacggaggc acgcccgctt ctgctgaaag
     8581 gcaggagttg cgacgcattg tttgtcagtc agaaaaaaac gggcatttcc cggcagttgg
     8641 cgtggatgat tgtcaaagaa tatgccggca gggcaggcat cgggcacatc agcccgcaca
     8701 gcctgcgcca cgcctttgcc acgcatctgg tgcggcacgg attggatttg cgcgtagttc
     8761 aggatatgtt gggacacgcc gatttgaaca cgactcagat ttatacccat gttgccaatg
     8821 tgcggctgca tagcgtggtt aaggaacacc attcccgaaa ctgaggcttc tgcttcggtt
     8881 taataagaaa tcaatcgaaa atgcaaactt gataaaaaat taccaagaca aaccgtgtat
     8941 gccgaccttg caatgcgaac cgtttttatt tatatttgcg tacgataata aaagtcgcta
     9001 tcggtacgat agtttgagaa cacacggagc acaaaatgtt tgtctgcatc tgcaatgccg
     9061 ttaccgacca tcaaatcaag gaaaccatcg ctgccggcgc gaccacaatg ggcgatttgc
     9121 agtcgcaatt gggcgtggcg agctgctgcg gctgctgcgg ggagtttgcc gcttcgtttc
     9181 tgacggcgca caacgcgcaa ccgacggtta cggcgggtat caacgttcaa gcgtaaaacg
     9241 gttttggaaa tgccgtctga actgttcaga cggcattttt gctgtttttg gcaggacttg
     9301 agtatcatct tcctcgaaga cattgttttt tcccaaatag accatgattc tgctgcgtct
     9361 aaggctttgg cgtgtgcaaa ttgacagata aggaaacgcg gatgaaattg accttgatgt
     9421 ttcgtgaata ttgcagcttg tgccacaaaa tgcgcgatgc actcaaacct tttcaggatg
     9481 aatacgggtt cgggctggaa gtggtcgatg tggatgaaaa tcctgttttg gaagaaaaat
     9541 acaatgagct gattcccgtt ttgttggcgg gagatgagga aatttgccac tggtttttgg
     9601 atgaggacag gttgaaacag tttctcgaac ggtaaaaaat gccgtctgaa gcaggacttc
     9661 agacggcatt tttttcaaat caacgttctt tacgtttttg tggggctgat gacctgccgg
     9721 taaaggaagc acgtttggat gcctggtaaa ttgccaatct tctacggtgt tgcatattga
     9781 ccctttcttt attttatttg tcggttggga gaattcttaa tttattgatt ttttcaataa
     9841 aaattagaaa atttattgtg agatgtcatt gttggcaatc atatcatgtt ttgctgttga
     9901 tggaagcatg attgtgtaaa gatgatatgt gtttgtgtaa tcagtagatt ttataagaaa
     9961 accgccaagg cggtgcttgg cggaggaaac ggaggggcgg gcggcatcag agcagcttgg
    10021 aaacgacctg tgcaagggct ttttgccagt ctgacggttt gatgccgaag tcgttttcga
    10081 ttttgcggca gtccaaaatg ctgtatgcgg gcctgggggc ggcagccgca gagccttcat
    10141 ccgaaacggc tttcagttcg ggaacgggaa aagaggcatc ctgttgcaat gccgtctgaa
    10201 agatgtgccg ggcaaattcg taccaggata cggatttgcc gccggcgtag tggtaaatgc
    10261 cgcgaacggg attggactgc tgcaacaggc ggatgatggc ggcggacaag tcgccggcat
    10321 aggtcgggca gccgatttgg ttgtggacgg cggacagcgg ggaacgttcc cgcgcaaggt
    10381 tcagcatcgt gcggacaaag ttgtccccgt attcgctaaa cagccaagaa gtccgcagga
    10441 taaggctgtc gggattggca gacagtgcga gcagttcgcc ggcggttttg gattgtccgt
    10501 atacattgga agggttggta aagtcgcttt cctgatgggg tattttccct tttccgtcaa
    10561 agacatagtc ggttgagatg tggatgaatc gggcatgggc gcgatgtgct gccaaggcaa
    10621 ggttgtaaac ggcggaagca ttgacggcaa atgccgctgc cgcatcgcct tccgctttgt
    10681 cgacggcagt ataggcagcc gtgttgacaa tagcgtcggg ttggaaactt ttgaccatgt
    10741 tgcagacggc atcggcatcg gtaatgtcta gggatgcgga atccgtcgca atggtttccc
    10801 agtcttcagg aagacggtcg cgcaggcagt gtgccagttg gcttttcgag cctgtcaata
    10861 ggattctcat gaggtatttc ctttggtgaa agtgtattgt aggacttgct gtcggtatta
    10921 tagtgccaaa actttgccga cggttgacgg gttggctttt tgtgccatgg gtattgtttt
    10981 gcgccgactt cggctagaat atcggtttgc gattcaaacc tgtcgggtcg gtattttcaa
    11041 gatattttca ggggggtgcg aaatgagtga gattggtttg gtaaagattt attccggaaa
    11101 agtgcgcgat ttatatgaaa tcgacgataa acgtatgctg atggtcgctt ccgaccgcct
    11161 gtccgcgttt gatgtgattt tggacgcccc gattccgggt aagggcgaaa tcctgacgca
    11221 gatttccaat ttttggttta aaaaactggc gcatattatg cccaaccatt tcaccggcga
    11281 taccgtgtac gatgttttgc ctgaaaacga agccaaagct ttggaaaaac gcgccgttgt
    11341 agctaaaaaa ctcactccgg tgaaagtgga ggccattgtg cgcggttatc tggcaggcag
    11401 cggttggaaa gattatcaaa aaaccggctc ggtttgcggt attcggctgc ccgaaggtat
    11461 gcgggaagcg caacaactac ccgaagtgat ttttacgccc tcaactaaag ccgcagtcgg
    11521 cgatcacgat gaaaacatca gctttgaaga atgcggacgc attatcggta aagaattggc
    11581 ggaagaagtg cgcgacaaag cggttcggct ttacaccgaa gcggcggaat atgccaaatc
    11641 gcgcggcatc atcatttgcg ataccaaatt tgaattcggt ttggatgaag aaggtacgct
    11701 gacgctgatg gatgaggtat tgactcccga ttcgagccgc ttctggccgg cggatcaata
    11761 tgaagtcggc atcaatccgc cgtcttttga caaacaattc gtccgcgact ggctggaaca
    11821 aagcggttgg aacaaaaaag cccctgcgcc taaagtgcct gccgatgtaa ttcggaaaac
    11881 ggtcgagaaa tatcaggaag cattgacttt actgacacaa gattgatttt gaagactgaa
    11941 ggccgtctga aaaggatatg tttcagacgg cctttttatt gtatcaatac tggattttaa
    12001 ggatggttgc ctttataatc cgcaattgtt ttcagcgtcc gaaatgccgt ctgaaagctt
    12061 gtttacaacc tgccgcacgg tctgaaaccc taactatgca cattcggatt ttagtgtgca
    12121 ttattagtgt tttagcagtg cggtattttg aaaggaacaa tgatgttcaa taaatacgtt
    12181 aagaccttcc aatacggtaa tcaaaccgtt actttggaaa ccggcgaaat tgcccgccaa
    12241 gctgccgctg ccgttaaagt atcgatgggc gacactgttg tttttgttgc cgttaccacc
    12301 aacaaagagg tcaaagaagg tcaggacttc ttcccgttgg ccgtcgatta tttggaacgc
    12361 acttatgccg caggcaaaat ccccggcggc ttcttcaaac gcgaaggcaa acaaagcgaa
    12421 aaagaaatcc tgaccagccg tctgatcgac cgcccgattc gtccgctgtt ccccgaaggt
    12481 ttctaccacg acatccaaat cgtagcgatg gttgtgtccg tcgatcccga aatcgattcc
    12541 gacattcctg caatgttggg cgcgtccgcc gcgctggtgt tgagcggcgt accgttcgcc
    12601 ggcccgattg gcgcggcgcg cgtgggttat ataaacggcg tgtacgtttt gaacccgact
    12661 aaagccgagt tggcgaaatc acaattggac ttggtggtcg ccggtacttc caaagccgtg
    12721 ttgatggtgg aatccgaagc caaaatcctg cccgaagacg tgatgttggg cgcggtggtt
    12781 tacggccacg atcaaatgca ggttgccatc aatgcaatca atgaatttgc cgacgaagtg
    12841 aatccggaag tttgggattg gaaagcacct gaaaccaatg aagaactggt tgccaaagta
    12901 cgcgggattg ccggcgaaac cattaaagaa gcgttcaaaa tccgtcaaaa acaagcgcgt
    12961 tccgccaaat tggacgaggc ttggaatgcg gtaaaagaag ccttgattac cgaagaaacc
    13021 gacacgctgg cagccaacga aatcaaaggt attttcaaac acttggaagc cgatgtcgtc
    13081 cgcagccaaa ttttggacgg acaaccgcgc atcgacggcc gcgacacccg caccgtccgt
    13141 ccgctgaaca tccaaaccgg cgtattgccg cgcacgcacg gttccgcact gtttacccgt
    13201 ggcgaaaccc aagcccttgc cgtagcgact ttgggtactt cgcgcgacga acaaatcatc
    13261 gacgcgttgt ccggcgaata taccgaccgc tttatgctgc actacaactt tccgccgtac
    13321 tctaccggcg aagtgggccg catgggcgcg ccgaaacgcc gtgaaatcgg tcacggccgt
    13381 ttagctaaac gtgcgttgtt ggccgtattg ccgaaacctg aagatttcag ctacaccatg
    13441 cgcgtggtct ccgaaattac cgaatccaac ggctcttcct ctatggcttc tgtctgcggc
    13501 ggctgtttga gcctgctgtc tgccggcgtg cctttgaaag cacacgttgc cggtatcgcg
    13561 atgggtctga ttttggaagg caataagttt gccgtcctga ccgacatttt gggcgacgaa
    13621 gaccacttgg gcgatatgga ctttaaagtg gccggtacga ccgaaggcgt taccgcgctg
    13681 caaatggaca tcaaaatcca aggcattacc aaagagatta tgcaaattgc tttggcacag
    13741 gccaaagaag cgcgtttgca catcttggat cagatgaaag ctgccgttgc gggtccgcaa
    13801 gagctgtccg cacacgcgcc gcgtttgttc accatgaaaa tcagccaaga caaaatccgt
    13861 gatgtgattg gtaaaggcgg cgaaaccatt cgttcgatta ccgctgaaac cggtactgaa
    13921 atcaatatcg ctgaagacgg taccatcact atcgctgcga ctactcaaga agcgggcgat
    13981 gcagcgaaaa aacgcatcga agaaatcact gccgaagtgg aagtgggcaa agtgtacgaa
    14041 ggtactgtgg ttaaaatcct cgacaacaac gtcggcgcga ttgtcagcgt gatgccgggc
    14101 aaagacggtt tggtacacat cagccaaatc gcacacgagc gcgtacgcaa tgtcggcgat
    14161 tacctgcaag tcggtcaggt ggtgaacgtg aaagcattgg aagtggatga cagaggccgt
    14221 gtccgtctgt ccatcaaagc cctgctggat gcgcctgtcc gtgaagaaaa tgccgccgag
    14281 taacgcttag ggtgaaaata ccgtctgaac aggtttcaga cggtattttt tacgggtatc
    14341 gggaatgaat ggggcttaca gccacaggac ggcaagcttc cataatgccc ataatgatac
    14401 ggataatcct gtacacaggc ggatatatcg gttttgcatg atttttttca gttgcaggga
    14461 aaaaatgccg attgccaaaa gattgggcag cgtacccagt gcaaaggcaa gcatatacag
    14521 tccgccggtt gtcgcactac cgcttcccag cgcgtaaagt gatgcgctgt aaaccagtcc
    14581 gcacggcagc cagccccata atattccgac agcaaggcag gcgggtatgg attttatggg
    14641 cagcagccgg ttgagtatcg ggttcaggtt gcgccatatc ggtttgccga ttttctcgat
    14701 ttttgccgcc aaggaagaaa taccgctcaa gtataagcct aaaaagagca gcaggaggtt
    14761 ggaggctgtg tataaaatat tttgcaggac gcgggtttgg tcgagtgaaa tgccgagttg
    14821 tccgattaat ccgagcatca gaccgattgc cgtatagctg cttatccgtc ctgtgttaag
    14881 cagcagaatc agccaaaagc ggttgatatg cggggggagt tggagcgcaa acgcgctgct
    14941 taatccgccg cacataccga tgcagtgagt tccgccgaag aaaccgagca ggaacagggt
    15001 gaggaaagtg atgtcgtggt tcatggatgg ctcatggtca gatgtttgtc ggggaaaacg
    15061 gacaatttcc ggcagtccga ccaatagggt ttgacgaggg cattccccgt gctgttaaag
    15121 tcttgaataa ggatgcggtt tgtgtcatga atttcgacgg tcctgtaaaa tccgcccttt
    15181 gctgtgctgt cggcgggttt gccgtgtgcg tcaaaataca ggacggtgcg gttgtgaaga
    15241 tgcgcgcaat ttgaaacggc ggggcttgcc ggtatgtttc gggtgcaggc ggcaagggtt
    15301 gcccaaggga aaagcagcag gaacaggcgg gacattgtgt ttcttgtaag gggtaacaaa
    15361 cagtataatg gctcatttta atcctcaggc ggcgggagat ggaagcattt cccttcgggc
    15421 tcggggattt cggattcgga agcaacagac aatacgggat ttcggaacaa tatgaaaact
    15481 ttgaaattta ccaaaatgca cggtttgggc aacgatttta tggtgattga cgcggtcagt
    15541 caggatttta cccccgagga cgtgccgatt gcggcatggg cggaccgctt ccggggcgtg
    15601 ggcttcgacc agcttttggt ggtcgggtgt tcggaaaccg aaggcgtgga tttccgttac
    15661 cgtattttca atgccgacgg tagcgaggtc gggcaatgcg gcaacggagc gcgttgtttt
    15721 gcccgttttg ttgcagacaa gggtttgacc gataagaaag aaatttgtgt tgaaacggcc
    15781 aagggcatta tttttccgaa attgtccgat aacggtatgg ttacggtcaa tatgggcaaa
    15841 ccgaagttta tgccgtctga aataccgttt gtccccgaat cgggcgaggg ggatgatgcc
    15901 tgtatttacg gggtgcatct cgaatccggc attcagcctg tcagctgcgt caatatgggc
    15961 aatcctcatg ctgtgattgt agtcgatgac gtggaatgcg cgccggtgcg cgaaaccggt
    16021 tcgcgtatcg aaccgcacag gcagtttccc gaacgcgtca acgtcggctt tatgcaggtt
    16081 gtcggacgga cggcaatccg tttgcgcgtg ttcgagcgcg gcgtgggcga aacccaagcg
    16141 tgcggtacgg gcgcgtgtgc ggctgtggtg gcgggtatcc gtctggggct gttggatgaa
    16201 gggaaaacgg tagaggtggt tttgccgggc gggactttat atatcgaatg ggcctgcggc
    16261 ggcgatgtga tgatgaccgg ccctgcggaa accgtgtttg aaggcgagtt ggcgtattca
    16321 tgattttgct gcatttggat tttttgtctg ccttactgta tgcggcggtt tttctgtttc
    16381 tgatattccg cgcaggaatg ttgcaatggt tttgggcgag tattgcgttg tggctcggca
    16441 tctcggtttt aggggtaaag ctgatgccgg ggatgtgggg aatgacccgc gccgcgcctt
    16501 tgttcatccc ccatttttac ctgactttgg gcagcatatt ttttttcatc gggtattgga
    16561 accggaaaac agatggaaac ggatggcagg cagaccccga acatccgctg ctcgggcttt
    16621 ttgccgtcag taatgtatcg atgacgcttg cttttgtcgg aatatgtgcg ttggtgcatt
    16681 attgcttttc gggaacggtt caagtgtttg tgtttgcggc attgctcaaa ctttatgcgc
    16741 tgaagccggt ttattggttc gtgttgcagt ttgtattgat ggcggttgcc tatgtccacc
    16801 gctgcggtat agaccggcag ccgccgtcaa cgttcggcgg ttcgcagctg cgactcggcg
    16861 tgttggcggc gatgttgatg caggttgcgg taacggcgat gctgcttgcc gaaatcggca
    16921 gatgattgtg atgccgtctg tatcggaaca aaaagttata aagaaataaa ttttgggtat
    16981 tggtttttta ggcggcatag gtttaggata aagctatatc cgaaatttgt ttatggttcg
    17041 gcgcaaatcc cctgcaatcg gacaggatac cgatggggat tgcgccttac tgtcgaaacc
    17101 ttattattca ggagcagaag atgaaaattg caaacagcat caccgaattg atcggcaaca
    17161 cgcctttggt caaactgaac cgtttgacca aaggtttgaa ggcagaggtt gccgtgaaac
    17221 tggaattttt taatccgggc agcagcgtca aagaccgcat tgccgaagca atgatcgagg
    17281 ccgccgaaaa agcgggaaaa atcaacaaaa acaccgtcat tgtcgaagca accagcggca
    17341 atacgggtat cggtttggca atggtatgtg ccgcacgcgg ctacaaactg gcgattacca
    17401 tgccggaaag catgagcaaa gagcgcaaaa tgctgttgcg cacgtttggc gcggaactga
    17461 ttctaacccc cgccgccgaa ggtatggcgg gcgcgattgc caaagcgcaa tccttggtgg
    17521 acgctcatcc agacacttat tttatgccgc gccagttcga caatgaggca aatcccgaag
    17581 tccaccgcaa aacaaccgcc gaggaaattt ggaacgatac cgacggtaaa gtcgatgtct
    17641 tcgttgccgg cgtcggcacg ggcggtacga ttaccggcgt gggcgaagta ttgaaaaaat
    17701 acaaacccga agttaaagtg gttgccgtcg agcctgaggc ttcccccgta ttgagcggcg
    17761 gcgaaaaagg tccgcacccg attcaaggta tcggcgcagg ttttattccg accgttttga
    17821 ataccaaaat ctacgacagc attgccaaag tgccgaacga agcggctttt gaaaccgccc
    17881 gtgcaatggc ggaaaaagaa ggcattttgg cgggcatttc ttccggtgcg gcggtttgga
    17941 gcgcgttgca gcttgccaaa cagcctgaaa acgaaggcaa gctgatagtc gtgctgctgc
    18001 cttcttatgg cgaacgctat ctctctacgc cactttttgc agatttggca taatgcttta
    18061 atcggattgt cgaaacattc agacgcattt ttcggtatcg gtgtaacgcc gtgccggaaa
    18121 atgcgttttt gcatatatac cgaaaacgcc ggttgtgttt taatcaggtg ttggcgccgc
    18181 cgcattgctt gagggaaata ttttttattc gggttttatc cggcaggacg gattttgccc
    18241 caacggaaaa tagccggcct gcccgtaaag tcagccgttt gtccgggcgc agccggggct
    18301 ttgggcttca gacggcatat tttcggaatg gcggcacttt tgccggcggc gcggcagcca
    18361 tatggggaag ggaggggata ttgtggtcgg taacggaaaa aaaatatgcc gcaccattgc
    18421 tggtgctggg ttgcgcggtg ttcggattgg gcagcctgat tgtcagatcc gtccccgtcg
    18481 gcccgtatgc aatcgcattt tggcggttgc tgatttcggt attcgtattt tggtttttgg
    18541 cacggttttt cggacaaaaa ttcccaaaaa acagaaaaac cgtccgatat gccctgacgg
    18601 cgggcgtgtt tctcgctttc gatttggcac tttggcacga aagcatacac gcggtcgggc
    18661 cgggtatttc caccctgctc aacagcctgc aaatcttttt cttgtcggca atcggtgttt
    18721 tctttttcgg cgagcgtttg ggcaggctgc aggcggcaag cttgatgtcg gcggttgtgg
    18781 gcgtggcgat gattgccggt gcggaattcg gctacaacgg taatgcggtt tggggattcg
    18841 ccagcggttt ggtgtccggg ctgatgcttg ccctatctat ggtgttcgtc cgcaagactc
    18901 atgaagtcga acaggtggcg cttttccctt caatgatgat tttgagtttg ggcggcgcgg
    18961 catcgccgat tgttccggca ttgctgatgg acggcggcgc actttatccg accacttgga
    19021 aagattcggg tttggtgctt gtgtacgggg cgtggtgatg cagtgttttg cgtgggcgat
    19081 ggttgcctat gcgattccgc tgctttccct gtcgctgacg gggctgttgc ttttgtccga
    19141 accggtcgcc gccctgttca tcgattattt cggattgggc aaaacgattg aaggcgtgca
    19201 gtgggcaggg gtggtgctga cgctttcggc gatttacctc ggttcgctga aacagccgtc
    19261 cgagctttga ctccggtcgg catcagattt tctgccgtgg caaacctgcg tacgattgga
    19321 ctggcaggca gtcaaaaccg gcaagaccaa acatgccgac aacggcgcgg agcggaaaat
    19381 gccgtctgaa gccctttcag acggtatcgg ggcgggcaga atcaaaaaag ccgcctccgc
    19441 aggaatggca atgcaactaa aataatgtcc gtatttccat atgccgccca caccgtcagt
    19501 cggacgaaat gccctttatt caatgggatg ggtttccaca caaacggaaa taaccggctt
    19561 ttcacttgcc gacggataaa atcaaccgat gatttaaaca caggcaaatc atacgccgcc
    19621 cgaacgcatc atttttccag gcaatccata tgtgccgccc atatcgataa tgccggcaag
    19681 gcggtgcata tagtggatta aattcaaatc aggccaaggc gacgaagccg cggacagtac
    19741 aaatagtacg gcaaggcgag gccaggctgt actggtttag atttaatcca ctatattatc
    19801 cccgaggctg ccggtaggga aacaaggcgt ttgatgataa aaatgccgtc tgaaacaggg
    19861 ttcggacggc atttttgatg ttccggcgta attttccggc ttaacggatt gccgtgccgg
    19921 ccctgccgaa atcgccgaag ttcatcaaaa tgaacattgc cttgccgaca accagcttgt
    19981 catccacaaa gccccagtag cgcgaatcgg cactgttgtc gcggttgtcg cccatagcga
    20041 aatagcgtcc ttcgggaact ttgcacacga aaccgctgcc gtcgtcggca tattggcaat
    20101 ggttcagacc gctttgatct atggaatatc cgttttcaga cataatatcg gaggtatatt
    20161 tgcccaatac gggcagggaa acggcaggct gtccttcttt tttcagaata ttgaaggatt
    20221 tgccgtccag accgctgcgg aacatatccg tgttgtggat ttcggatggg tcggtatcgt
    20281 caggataacg gtatgtgccg tcaggaatgt cggaagccgg ttttccgttt accgtcaaaa
    20341 ctttatctcg atattcgaca atgtcgcccg ggatgccgac aatacgcttg atgtaggtca
    20401 tctccggctg caaaggatag ttaaaaacaa cgacatcgcc ccgttcgatt ttgcccgtgg
    20461 ggatgaatac attgtttaaa acgggtacgc gcaggccgta ggaaaatttg ccgaccaaaa
    20521 tgaaatcgcc cttgattaat cccgggcgca tcgagctgga cgggatttgg aacggttcgg
    20581 caataaacga ccggatgagg aacaatacca aaacggtagg gaagaaactg ccgaaataat
    20641 cgccgaagtg gctgctttcc gagatttcgg gatgagtctt caggcggtat ttatataccc
    20701 cccaagccgt accgcacaat acaacgaaaa tcaggaaaac ggcggtaaag ctcataaaca
    20761 gggacaaagc ggcaaacaca ccgaccgccg tcaggatata ggcgtattcg aggccggaac
    20821 tccattcacc gttttcctgc cgtttcttgt cgcttttgaa ataaaggatg atgccggcaa
    20881 gcagcgcggc agccgcgccc gacattagca ttgtgttcat tgttgttcct taacggttaa
    20941 aaacccgccc ggccgtgcaa ccgttttaag gcggcaaatt gcaaaatttg tttgcgggcg
    21001 cgtgccgctg aaatcagggc ggtttgagag gtgttcccga cgcgcccgcc ctatgtgccg
    21061 aaatattatt tgtcgctcac ctgcaaaatc gccaagaacg cgctttgcgg aatttccacg
    21121 ttgcccactt gtttcatacg gcgtttgcct gctttttgtt tttcaagcag ttttttctta
    21181 cgcgtaatat cgccgccgta acatttcgcc aaaacgtttt tacgcagggc tttaacgttt
    21241 tctcgggcga taatccggct gccgatggcg gcttggacgg cgatgtcgaa catttggcgc
    21301 ggaatcagtt cgcgcatttt cgatgccagc tcgcggcctt tgtgaaccgc gctttgacgg
    21361 tgtacaatca ggcttaaggc atcgactttt tcgccgttaa ccataatgtc gagcttaatc
    21421 aaatcagacg gttggaattc tttgaaatgg tagtccaacg aagcataacc gcgcgaagtg
    21481 gatttgagtt tgtcgaaaaa gtccattacg acttcgttca tcggcaagtc gtaagtcagc
    21541 atcacttggc ggcccatata ctgcatattg acctgcacgc cgcgcttttg gttacacaaa
    21601 gtcatgacgt tgccgacgta ttcctgcggc acaaggatgg tcgcggtaat aatcggctcg
    21661 agaatggttt cgatgctgcc gatgtcgggc agtttggacg ggttttcaac ttcgattttt
    21721 tcgccgcttt tcaacacgac ttcgtaaacc accgtcggcg cggtggtaat caaatccatg
    21781 tcgaactcgc gttccaagcg ttcctgcacg atttctaaat gcagcaggcc taagaagccg
    21841 cagcggaagc cgaagcccaa tgcttgagaa acttcaggct cgaatttcaa cgaagcatcg
    21901 ttcagctgca atttttccaa agcgtcgcgc aaggcttcgt aatcgtggct ttccacagga
    21961 tagagacccg caaatacttg gctttgtact tcttggaaac cgggcagcgg ctcagtggca
    22021 gggttggcaa ccaaagtaac cgtatcgccg actttcgcct gtcccaattc tttcacgccg
    22081 gtaatcaaaa aacccacttc gccggctttt agttcttgtt tttgaaccga tttcggtgtg
    22141 aatacgccca gctgctcgac ctgcgtttcc gccttggtgc tcataaagcg cactttgtct
    22201 ttcagtttga tggtgccgtt tttcactcga atcagcatga ccacgccgac gtaattgtca
    22261 aaccacgaat cgacgataac ggcttgcagc ggcgcgtttt cgtcgccggt cggcgcaggg
    22321 attttggcaa cgatttcttc caaaacgtct tccacgccga tgccgctttt ggctgaacac
    22381 tgcaccgcgc cgacggcatc gatgccgatg atgtcttcga tttcctgttc cacacgctcg
    22441 gggtcggcgg cgggcaggtc gattttgttc aaaacgggca cgacttccac gcccaaatca
    22501 atcgcggtat agcagttcgc cacggtttgc gcttccacgc cctgcgacgc gtcaacgacc
    22561 aaaagcgcgc cttcgcaagc cgacagcgaa cgggaaactt cgtaagagaa gtcgacgtgt
    22621 cccggcgtgt caatcaaatt aagctgatac acctgcccgt cgcgtgcttt gtagttgagt
    22681 gcggcggttt gcgctttgat ggtgatgccg cgctcttttt cgatgtccat ggaatcgagc
    22741 acctgcgtac tcatttcgcg caaatccaaa ccgccgcaat attggatgaa gcggtcggca
    22801 agcgtcgatt tgccgtggtc gatgtgggcg atgatggaga aatttcggat atttttcatt
    22861 gagttgtttt taatgctgaa cggtaggttt tagaaatgcc gtctgaacaa acagcggtac
    22921 gtccgaatat cgggcgcaac gtggaaatag cccgatattc taacggaaaa ccgctgtttt
    22981 ggcatgattt gataaaggtt ttataaagat ttgacgattt ctgccaccat ttttgcagaa
    23041 cttgccgccg ccgttttcaa aaactcttca aagctgatgt ctgccttttc gtccgccgaa
    23101 tcggaaacag cgcggatgat gacgaacggc gtttccaact gatgacaggt ttgggcaatc
    23161 gccgccgctt ccatttccac tgctttgact tcggggaagt gcttgcggat ttccgccacg
    23221 ccttcgctgc tgtggacgaa gcggtcgccg ctgacaatca ggccttgttc tacctccgcg
    23281 ccttcaaacg tccgcgccgc ccgttttgcc gtttcaatca atatgccgtc tgaagcgaac
    23341 cttgccggca gttgcggcac ttgcccccgg gcatagccga atgcggttac atcgacatcg
    23401 tggtgctcgg tttccgtgcc gatcaccacg tcgccgactt tcaaaccctt gcccaaaccg
    23461 cctgcgctgc cggtgttgat gacgcagtcc ggtgcgaatt gacggataag ccaagccgtt
    23521 gcaaccgccg cgttgacctt gccgatgccg ctcaatgcaa gcactatgcg ttttcccgcc
    23581 aattcgcctt cataggcgga aaatctgccg aaagagacgg ctttgacatt ttccatcatc
    23641 tcgcgcaaaa gctcgatttc ctgttccatc gcgccgataa cggctactgt tttcaaagac
    23701 atattgctaa cctgctgtga atttcggata gaacgccgaa ttatacacgc taacacggca
    23761 ggattgagtg gaggcggttt gtccgtgccg tctgaaacgg tttcagacag cacggcggat
    23821 ttttggtaga atgggaaggt acagattgtt tgaagattgg gggacgagga tgtttaccga
    23881 tgaaaatatg actgcaaagg aagaactgtt cgcatggctg cgccatatga acaaaaacaa
    23941 aggttccgac ctgtttgtga cgacccattt cccgcccgct atgaagctgg acggcaaaat
    24001 cacccgcatc acggacgaac cgctgacggc ggaaaaatgt atggaaatcg ccttttcgat
    24061 tatgagtgcg aagcaggcgg aagaattttc atcgaccaac gagtgcaact tcgccatcag
    24121 cctgccggac accagccgct tccgcgtcaa tgcgatgata cagcgcggtg cgacggcgtt
    24181 ggtattccgc gcgattacca gcaagattcc caagtttgaa agcctgaacc tgccgccggc
    24241 cttgaaggat gttgcgctga aaaaacgcgg gctggttatt tttgtcggcg gcaccggctc
    24301 gggcaaatcg acttcgctcg cctcgcttat cgactaccgc aatgaaaatt cgttcggaca
    24361 catcatcacc atcgaagatc cgatcgagtt tgtccacgaa cacaaaaact gcatcattac
    24421 ccagcgcgag gtcggcgtgg acacggaaaa ctggatggcg gcgttgaaaa atacgctgcg
    24481 tcaggcgccg gatgtgatcc ttatcggtga aatccgcgac cgtgaaacaa tggactacgc
    24541 catcgccttt gccgaaacgg ggcatttgtg tatggcgacg ctgcacgcca acagcaccaa
    24601 tcaggcgctc gaccgcatca tcaacttctt ccccgaggag cggcgcgaac aattgctgac
    24661 ggatttgtcg ctcaaccttc aggcgtttat ttcgcaacgc ctcgttccgc gagacggcgg
    24721 caagggcagg gtggcggcag tcgaggtact gctcaattcg cccctgattt cggagttgat
    24781 tcacaacggc aacatccatg aaatcaaaga agtgatgaaa aaatccacta ccctgggtat
    24841 gcagaccttc gaccaacacc tttaccaatt gtatgaaaaa ggcgagattt ccttgcagga
    24901 tgccttgaaa aatgccgatt ccgcacatga tttgcgtttg gcggtacagt tgcgcagccg
    24961 cagggcacaa agttccgacc ccgatttgga actgctctga tggcggtatg gatttccgga
    25021 cgggcagttt gacatgattt atccgtggca tcaggaacaa tggcggcaga ttgccgagca
    25081 ttggacgagc cgtcctaatg cgtggctgtt tgttggcaaa aaggggacgg ggaagactgc
    25141 gtttgcccgc tttgcggcga aggcgctgtt gtgcgaaacc cctgcaccgg gctgcaaacc
    25201 ctgtggcgaa tgtatgtcct gccatctgtt tggacgggga agccatcccg atttttacga
    25261 aatcacgcct ctggcggacg aacccgaaaa cggacgcaaa ctgttgcgga tcaaaatcga
    25321 tgccgtcagg gaaatcatcg ataatgtgta cctgacttcg gtacggggcg gtttgcgcgt
    25381 gatcctgatt catcctgcgg aaagtatgaa tgtccaagcc gccaacagtt tgttgaaagt
    25441 gttggaagag ccgccgccac aagtggtctt tttgctggta agccacgcgg cggacaaggt
    25501 tttaccgacc attaaaagcc gctgccggaa gaaggttttg cccgctcctt cgcatgggga
    25561 agcattggcg tatctgcgcg acaggggtgt ggcggagcct gaggaacgtc tggctttcca
    25621 ttcgggcgcg ccgctgtttc aggaggaagg cgagcttcgg gaattgaggg caaagctgct
    25681 ggagatattg gcagagccga gattgttgaa gattttggat tacgccgcgc ttttcgataa
    25741 ggaaaaactt ccgctcgccg tatttgtcgg gtggatgcag aaatggctgg tcgatttagg
    25801 cttgtgcctg caacacatga aacccgtcta ttatcccgct tatgaagaca ggctgcttca
    25861 gacggcatcg ggtttccgtc cgcgcaatgt atttgcggcg gaggatatgc tcaaacagct
    25921 tgccccttac gggtttcata ctttaaatgt caaaatgcag atcgagcatc tgctcatcaa
    25981 ctatttggaa ttgaagaaag agaacaggtg aattatgtca gacggacaaa atattccggc
    26041 aaaaatgatg tcgttgcagc tgaaagacat gaatctgctg tacagctcct atatgccgtt
    26101 tttggaacac ggcggtctgt ttgtgcagac cgacgacgta ttttccatcg gggacgatat
    26161 tctgcttgcc gtagaaatcc tcaacttccc caaactgttc ctgccgacca aggtcgcctg
    26221 gattaatcct gcgagtactt cctccaaacc caaaggggtg gggctggcat tcacaaaaca
    26281 cgaaaactgc ctgaaagtca aagaccagat cgaagtcgaa ctgggcagca caatcagcgg
    26341 cagcagacct acgtttacca tgtaacgcta tgcatatcat cgattcgcac tgccacctca
    26401 attttgaagg tttgaaagaa cgcctgcccg aagttttgtc caacatggaa gcaaacggcg
    26461 tggggcaggc actcgccatc agcgtcagta gggaaagctt ctccgaagtc tttgccgtcg
    26521 ccgaagcgca cgaacacatc tattgcacca taggcgtaca tcccgacagc aaggaagccg
    26581 aagaattttc cattgcggaa atggtcgaag ccgccgccca tccgaaagtg gtcggcatcg
    26641 gcgagacggg tttggattat tactggtgca aaggcgattt gtcttggcag cacaaacgct
    26701 ttgcagacca catcgaagca gccaatcaaa ccggactgcc cgttatcgtc catacgcgcg
    26761 atgcggcggc ggacaccttg gcgattttaa aagaatgtaa aaccaattcg ggtgtgattc
    26821 actgtttttc cgaggatgtc ggttttgccc gggctgccat ggatttgggg ctttatattt
    26881 ctttctcggg aatcgttacc tttaaaaacg cccccttggt tcaggaggcg gcgaaatacg
    26941 ttccggacga ccgcatttta gtggaaaccg atgcgccgtt ccttgccccc gttcccaaac
    27001 gaggcaggca gaacgaaccg gcttttgtgc gtcatactgc cgaacatatt gccaaattgc
    27061 ggaatcaaac attggaacag gttgcggcat atacgacgga aaacttttac cggctgttta
    27121 aaaaagtacc cgatatgcgg gtcgtctgac cctgtgccga cgataaggaa aaccatgaag
    27181 gcaattcatc cgtatgcatg tccgcgctgc tgccggctgc ctgccaacac gtttcggaca
    27241 ggcatggcaa attccgcttc caaattctgc attgccaaag gcggcagacg ggaagtaaaa
    27301 aaagacgaaa gcggcggcgg atatgccctg tgccatttgc cggacagcag gattgtcgag
    27361 gagtgggaat attgccgttc gcaacattga tactgcgcga tatacggcaa atattgtggg
    27421 aagtttccgc ttttgcgtat aatgcgccct acctgacaaa ttttgtcaac tttatcaaaa
    27481 ggaataagcg atggcttcca tccacgacca aattaaagaa gtagtaacga cacaccgcgt
    27541 cgtattgttt atgaaaggta cgaagcagtt tccgcaatgc ggtttctctt cccgcgccgt
    27601 gcaaatcctg aacgcggcag gctgcaccga ttacgttgct gtcaacgtat tggaaaatcc
    27661 cgaagtacgc caaggcatta aggaatacag cgactggccg accatccccc aactttatgt
    27721 gaacggcgag tttgtcggcg gttcggacat cctgatggaa atgtatgagg caggcgagct
    27781 gcaagagctg ctgaaagcct gatggattcg gcaatgccgt ctgaacgtgt ttcagacggc
    27841 attttctttt ccggcaaatt aaaaaaaagt ataatgacgc gtctcaaaat cacactggaa
    27901 caccgcgatg aacgttaacg ttatcaacca tccgctcgtc cgccacaaac tgaccctgat
    27961 gagggaggcg gattgcagca cctacaaatt ccgtacgctg accaccgagt tggcgcgcct
    28021 gatggcatac gaggcaagcc gtgattttga aattgaaaaa taccttatcg acggatggtg
    28081 cggacagatt gaaggcgacc gcatcaaagg caaaaccctg accgtcgtcc ccatcctgcg
    28141 cgccggtttg ggtatgctcg acggcgtgct cgacctgatt ccgactgcca aaatcagcgt
    28201 ggtcggtttg cagcgcgacg aggaaacgct gaagcctatt tcctattttg agaaatttgt
    28261 ggacagtatg gacgagcgtc cggctttgat tatcgatcct atgctggcga caggcggttc
    28321 gatggtggcc accatcgacc tgttgaaaga aaaaggttgc cgcaacatca aagccttagt
    28381 cttggttgcc gcccctgagg gcgtgaaggc tgttaatgat gcgcatcccg atgttacgat
    28441 ttataccgcc gcgctggata gccgtttgaa cgaaaacggc tatattattc ccggtctggg
    28501 cgatgcgggc gacaagattt tcggcacgcg ctaactgact gattttcgga tttgatatga
    28561 atttccaaga ctatctcgcc acatttcctt caatcgacca tctgggcggt ttggatgttc
    28621 aggatgccga aggcaaaacg gttcaccaca ttcccgccgt tcagggcaag ctcggttcgc
    28681 tcaagctgta caatgccttg gcggaacgtt ttgatggaaa attgggtaaa gaagcggcag
    28741 aacagggttt gatatggttt gccgaacatg ttgccgacgc gcgcgcccat ccggggaagc
    28801 atccgaacat cgatttgctg gaaaatgtcg tgcaaagcgg cgaaaccctc ctgctcaaac
    28861 cgcttgccgc gcaataattt tcgaccatgc cgtctgaaat tcgtttcaga cggcattttg
    28921 tcggaaagaa gaccgtaaaa cgggcatttt cttttctatt tggctgtgtt ttaacaatcc
    28981 gttgattttt gcactaattt ttgataaaat cccttataac aaattgaact ataagggatt
    29041 ttataatgtc gggcgtaaca aaagaattag acattttaaa acaacttttt gaaaatttgt
    29101 ccgatacgga taaacaggct tttttgactt ctgtcagctc aaaagaacag gttaaaaaag
    29161 tcattgaacc aagaaaagtt acgaagtgcc cacattgcca atctacacat ttcgtcaaaa
    29221 atggcaaaga ttgtggcaac caacgttttt tgtgtcgtga ttgtaaaaag tcttttgttg
    29281 agcagacagg cactattctc tacaacacgc aaaaagacat tgaagtttgg gaaaaataca
    29341 ttcattgtat gattgaaaaa tacccacttc gcaaatgtgc tgaaatttgc aaaatcaatc
    29401 ttgctactgc gtttacttgg cggcacaaaa ttttggacgc acttcaaaac atgatgaatg
    29461 aagttgagtt agacggcatt gtgcaagctg atgaaactta ttcgacgatt tcttacaaag
    29521 ggcaccacaa aaattttaat ctgccacgtc ctgctcacaa acgaggaacg agagcaacga
    29581 aacgtggcat ttccaaagaa caagtttgtg ttccttgtgg tatcaattta gacggtaaat
    29641 ctgttgctag aattagcaat ttgggtaagc catctctaaa aaatatcaac tgattggcat
    29701 tgaaaacaac ccaaaaatgt ttgctcttgc cgccagtaat atgattttac gcggagacgg
    29761 taaagccaat ttacaccaaa gcagttgttt catgactgat tttcaggatt taatcaaaaa
    29821 tcccaaacct gaaacaggct taaaacgccc aaatgttggc tttctcaatc caccttacgc
    29881 ccaatccaaa agcgatgccg aattgcatga actttatttt gtcaaagaaa tgttggatat
    29941 gttggcagaa ggtggcacag gcattgccat tattccagtc agttgcgtga ttgcaccaag
    30001 caaagccaaa agcgaaattg tgaaatatca tcgcttaaaa gccgtgatgt ctatgccgag
    30061 cgaactgttt tacccagttg gcacggtaac gtgcattgtc gtatttgaag cccataaacc
    30121 gcattttcag acagtcgtga ttgacccgga cacacaagaa gaaatcagca cgaaaaaagc
    30181 ctgtcgcaaa acgtggtttg gctactggcg tgatgacggt tttgaaaaaa ccaaacactt
    30241 gggacgcatt gatttatacg accgctggca gggcattaaa gcgcgctggt tggaacatta
    30301 tttaaacaac gaagttcaca caggagaatc ggtaacagca tttgtaactg ataacgatga
    30361 atgggttgcc gaagcctatt tggaaactga ttattccaaa attacccgag cagattttga
    30421 gcaagtcgtg cgtgaatttg ctttatttca actactggga gcggaagtag ggccgactga
    30481 aaatttggat aatgaaagct atgaagacga tgacaataac gacttcggag acgatgaata
    30541 atggttgaat tgcaagagat ttttgatgtg agttacggtt caaaattaga tttgaataaa
    30601 atgagcagct tcaatccaac aatcaacttt gtaggcaggt caggcaaaaa taatggtgta
    30661 acagcatctg tggatttatt gaaaaataca aaaccttatc cagcaggatt attaactgtt
    30721 gcattaggtg gttctgtttt atctactttt ttacaaaaca aaccatttta tacagctcaa
    30781 aatgttgctg ttttaaatcc caaaacagaa atgacagaac aacaaaaact cttttattgt
    30841 gctgctattt ttgccaatgc ctaccgtttt tctgcttgcg gtagggaagc taaccgcacg
    30901 ttacgacaac tgtttgtgcc atctttggat gagatcccaa gctgggttga aagcgtgaat
    30961 ttaaatccat ctgcgggtgt aaccgaaccg aaattaaaag aaccgcttga tttgcctgtg
    31021 gttcgacaat caaaacgact tgatgaaatt tttactattc aaaacggcat tgccgcaacc
    31081 aaattaaaag aatttgaaca acggcaaaaa gacacggttg tctatatccg tcctgccagt
    31141 acgcaagcaa gaacgctacg aagttatatc gctcgtgata gcgttgatga aaaacatatt
    31201 tttccatgcc atacgctttt cacttctacc aatggcgaag gtagccatac ttatagctat
    31261 gtttcaactt gtgaatttgt agcaaatagt gatgtcgccg tattaacacc catacagtcc
    31321 gatatgccaa tagaagtaaa actgtattac gctaaatgta ttacagcaaa ccgctatctg
    31381 ttttcttatg gacgtaaacc caaaggcgaa aaattaaaaa gcattatgtt accgtatttt
    31441 gaccaacaag aagattttga ttatatctgc cgtttcattc acaccttgct atttagtaac
    31501 aatttgtaga ttaccaccct tataaacaaa acaacccgcc gtaatagatg aaatagagaa
    31561 ctgtttttta gtgtgaattc atcttaagca atcaacggat tgttaaaaca cagccttcta
    31621 tttcaggata cgggcaatga tgtttcaaca cacaggacga cacataaagc gccgccctat
    31681 gtgttgccct aatttgaaag gggttacacc cctttcaaat aaaatctgat gctgctgcca
    31741 cgaaggacgg atgtccgaat ggcggggttt caaccattaa ggaaattatg atgaaaaaaa
    31801 tgttcctttc tgccgcattg cttctgtcgg ctgccgccca aaccgtgtgg gcggatacgg
    31861 tattttcctg taaaacggac aacaacaaat acatagaagt tcaaaaaatc aaccgcaatc
    31921 tttacgaata ttcgttcggc agtgcggcaa aaaaagaaat tgccatacgc aacagcaaag
    31981 ctgacctgtt ggggcgttcc gacaggtggc aaggcatggg cagcggacgc cgggcaacga
    32041 tgaaattcca aaacggcgaa tttatgtaca ccgtatggac aggcttcgat tccgtgactc
    32101 atacggaaag cagcggtgtc gttgtggagc gtaggggcaa ggaagtcgca cgggtaggct
    32161 gtacgccgaa aaccgcgcag gcgaatttca acgatgacga tttttcccgg taatcggggc
    32221 ggataaggcg atggaaacag cgaagcccgt catgctgatt gtccgcccgt cgggcagggc
    32281 gaaggacgat gtggaagtct gccgtcgtgc cggatggcag gcggaagtgt tgagtccgat
    32341 tgaaatcgaa accgacgaag caagcctgaa acggctgccg gaaatgtatg cccgtgcgga
    32401 tgccgtgttt tgggtcagtc cggccgccgt tgaaaccgcc gtcccgtacc ttaacctttc
    32461 agacggcata aaggcgcaca ttgccgtagg gcaaggcagc cgccgtgcct tggcgcgtta
    32521 tgcgggcgcg ggcgtgtttg cacctgaaga cggcaacgac agcgaggcgg ttttgcgcct
    32581 gccggtttgg aacagtctgc ccgaaggtgc gcgcgtattg tctgtgcgcg gacacggcgg
    32641 gcgggatttt ctgatgaatg ccttgcagga gaaaggtttt cggacggagg tggcagaagt
    32701 ctatttcaga cggcataaac ctttgaactt tcaaaatttc caaaccgaaa atattgccgc
    32761 cgcctatatt acgtccacag aacttgtgca gtcgctgttt gcacagcttc cgccgcaatt
    32821 ttcccgattc ttcaaatcct tgctatactt tacccatcat ccgcgcattg cggaggcatt
    32881 gaagcgcgaa ggcgtgtgtt cggtcgaaac cgtccctacg ctggaagccg cgctttccta
    32941 ttcttccatt tccgtttcag acggcatggt ctttcccgga acctcaaacc aataaggagc
    33001 aaaacggtgg gcgaacctga aaacaaatca tccgaacccg tacgcgagat acaggcatca
    33061 aaagaaatgc cgtctgaaac ctcttcccca cgcaaagaaa acgaaacaga agtacacatt
    33121 cccgccgctc cttttatcgt caaacagtcc ggcagcaacg ctttggcggc ctgcgccttg
    33181 gtattggcgg cattgggttt gggtgcaagt ggttttttgt ttgtccaagg acagaatgtc
    33241 ttgaaaaacc aagagctggc attcaaccag aaaatcgaca aagccgcctt gggcgagtcg
    33301 gaaaacgccg ccctgctcaa agacaacctc aaccgccaaa gtgccataca gtccgaactc
    33361 gaccgtttgg acagcggtgt caaagccaac ggcgaacaaa tcctgatgac gcaaaaagcc
    33421 tatcgcgagc tgaccaaagg gcgtgccgat tggctggtgg acgaaaccga gaccatactc
    33481 aatctggcgg cgcaacagct ggtgttgacc ggcaatatcc aaacggcagt cggcgtgttg
    33541 gagcatatcg acagccgcct gtcccgtttc gatcaggcag agcttctgcc gatcaaacag
    33601 gcggtcagca gcgatttggc ggaactgaaa aaccgtccct atgtcgatat ttccggcacg
    33661 gcattgcgcc tcgaccggtt ggaaaccgcc gtatccggac tgccgctgat tttggacggc
    33721 gtattgaaac cgggcgtaca ggcccgcaac gaagccgttt ccgcttcatg gtggcagaac
    33781 gtatgggaaa aatccctcgg cacattgaag gggctggtcg aaatccgccg tttggaaaac
    33841 aacgatgcca tgctgatttc tcccgaacag gcatattttg tgcgtgaaaa cctgcgtctg
    33901 cgcctgttgg atgcgcgcac cgcattgatg cagcgcaacg gcgaagtcta tcagggcgat
    33961 ttgaacaatg ccgaagccgc cgtcagacag tatttcgatg ccaagtcgcc cgccacgcag
    34021 tcgtggctga aagaactggc ggaattgaaa acactggatg tacgcatgac ggcggatgac
    34081 ggcttgaaga ccagtttaaa tgccgtccgc gcctatcgcg acggtacgcg catgacggcg
    34141 gcggaaaatc aagaagcgga acaggcggct tccgaaccgg caaacgaaaa cacagcttcc
    34201 gaaccggctg ccgcatcgga tgtgaaggcc atagaagcac cgtccctgcc ttcggaacgc
    34261 aaaccggaac agcctgcaaa aaaacagccc gcaccggaaa aggcagggcg ttcgccgtcc
    34321 gctaaaggag aacgcgcatg aaaacggtag tctggattgt cgtcctgttt gccgccgccg
    34381 tcggactggc gctggcttcg ggcatttaca ccggcgacgt gtatatcgta ctcggacaga
    34441 ccatgctcag aatcaacctg cacgcttttg tgttaggttc gctgattgcc gtcgtggtgt
    34501 ggtatttcct gtttaaattc atcatcggcg tacttaatat ccccgaaaag atgcagcgtt
    34561 ccggttcggc gcggaaaggc cgcaaggccg cgcttgcctt gaataaggca ggtttggcgt
    34621 atttcgaagg gcgttttgaa aaggcggaac tcgaagcctc tcgagtgttg ggcaacaaag
    34681 aggccggaga caaccggact ttggcattga tgctgggcgc gcacgcggca ggacagatgg
    34741 aaaatatcga gctgcgcgac cgttatcttg cggaaatcgc caaactgccg gaaaaacagc
    34801 agctttcccg ctacctgctg ctggcggaat cggcactgaa ccggcgcgat tacgaagcgg
    34861 cggaagccaa tcttcatgcg gcggcgaaga tgaatgccaa ccttacgcgc ctcgtgcgtc
    34921 tgcaacttcg ttacgccttc gatcggggcg atgcgttgca ggttctggca aaaaccgaaa
    34981 aactttccaa ggcgggcgcg ttgggcaaat cggaaatgga acggtatcaa aattgggcat
    35041 accgccgcca gatggcggat gctgccgatg ccgccgcttt gaaaacctgc ctgaagcgga
    35101 ttcccgacag cctcaaaaac ggggaattga gcgtatcggt tgcggaaaag tacgaacgtt
    35161 tgggactgta tgccgatgcg gtcaaatggg tcaaacagca ttatccgcac aaccgccgcc
    35221 ccgagctttt ggaagccttt gtcgaaagcg tgcgcttttt gggcgagcgc gaacagcaga
    35281 aagccatcga ttttgccgat tcttggctga aagaacagcc cgataacgcg cttctgctga
    35341 tgtatctcgg ccggctcgcc tacggccgca aactttgggg taaggcaaaa ggctaccttg
    35401 aagcgagtat tgcactgaag ccgagtattc cggcgcgttt ggtgttggca aaggtttttg
    35461 acgaaaccgc acagtcgcaa aaagccgaag cacagcgcaa cttggttttg gcaagcgttg
    35521 ccggggaaaa ccgcccttcc gccgaaaccc gttgaaacct tcagacggca tcgttgaaat
    35581 gccgtctgaa aaccatttca cttgacaaac agactgacag aaaacccgta tgactctttt
    35641 aaaaaacgat accttcctcc gcgccctgct caaacagccc gtcgaataca caccgatttg
    35701 gatgatgcgc caggcggggc gttatctgcc cgaatacaaa gccacacgcg cgaaagcggg
    35761 cagcttcctc gatttgtgca aaaacaccgg attggcgacc gaagtcacca tccagccttt
    35821 ggaacgcttc gatttggacg cggcgatttt gttttccgac atcttgaccg ttcccgatgc
    35881 aatgggcttg gggctgtatt ttgccgaagg cgaaggcccg aaattcaaac gcgccttgca
    35941 acacgaatcc gacatcgcca agctgcacgt tcccgacatg gaaaaactgc aatacgtttt
    36001 cgacgcggta acttccatcc gtaaagcatt ggacggccgc gtaccgctta tcggcttctc
    36061 cggcagcccg ttcacgctcg cctgttatat ggtcgaaggc ggcggcagca aagaattccg
    36121 caccatcaaa accatgatgt actcgcgccc cgatttgctg tacaaaatcc tcgataccaa
    36181 cgcccaagcc gttaccgcct acctcaacgc ccaaatcgac gcgggcgcgc aggcggtgca
    36241 gattttcgac acttggggcg gcgtgttgag cgacgcggcg tttaaagagt tcagcctcaa
    36301 atacatccgc caaatcgttg ccggactcaa acgtgaaagc gaaggtcgcc gcgtgcctgt
    36361 tatcgtgttt gccaaaggcg gcgggctgtg gctggaaagc atggcccaaa tcggcgcaga
    36421 cgcattgggc ttggactgga cgtgcaacat cggcgaagca cgccgccgcg tcggcaacca
    36481 agtcgccctg caaggcaact tcgacccgtc cgccctcttc ggtacgcccg aatccatccg
    36541 caccgaggtc gcacgtatcc tgaccggcta cggacacggc agcggccatg tcttcaacct
    36601 cggacacggc atcaaccaac acgccgaccc cgaacacgcc aaaatcttag tcgacaccgt
    36661 acacgagctg tcgcggcagt atcacggcgg gtaagccggc aggaaaccgc ccgatatgcc
    36721 gtctgaagaa tcggaagttt tcagacggca tatgcttcat tttgcacttt ggtacaccaa
    36781 gccattgtgt ggtagcttga tattgggtaa ccaaaaatct tttccctgcc aacctttgtc
    36841 tttatttcct actacttgat aaagcgtaag gaataaatca ttgggatatt tttctcctaa
    36901 tttacggtcg tcttccgaaa gcattgtgcc ggttgctcga gaaagtttac gtccggtttt
    36961 gatcaaaaca taggttttaa aacttggacg ttgtgctttt aatgcttcta caccagcaat
    37021 aaatttttcc ttattccagt catctggaac ataacgaccc aatttatcta gaaccaagaa
    37081 taaatcttct atatctattt gatataaatc agattgaatt tcatctttca aaatagaagg
    37141 caaaattttg ttaatctccg ataaattatc ttcgtttggg tggagtggaa aataattcac
    37201 accacccaca atttgattga tactatcgaa tttcaataca ttttttcgag ttggattgat
    37261 atttttcgga taaataacct gaatattgcc gcctgaattt tttgcctgcc caataatcag
    37321 attatttgct tgattgagtt gtacaaagaa gtaatagaca tcgaacggaa tataaagccg
    37381 taataatgat ttatcgcggt catatccaaa aatacgcgaa tgttgccaga aagtatctgc
    37441 attcggtttt ttggcagtgc gactataata aaccgtttgt agtttcggaa tagtcaagcc
    37501 acgaccaatc acattaccac caataatgat gttgaaacct ttttctaagt caaaatctgt
    37561 ttctgtctgc gagttaacga caagagtaga gatttgttta ttctctaaaa gtgctgtcaa
    37621 tttttcgtat atttcatcaa agtgatgaat atcgggcttg gtcttttgca aatccaaata
    37681 actttcttta aagctacctg caaggtcttc cccattattg actgcttgca ctaagtcatt
    37741 taaaaaggct tggatcttct ttgaaaaggc ttgatgatct tgaattttat agcttggatg
    37801 tagtgcaaaa ttacagttag ccttatcaca tagcgcgaat tctgcacagg ttattagaaa
    37861 actaagcaat gcctgttttg ctccttcggc aatttcacca ctctcgtctt tcatatcatc
    37921 caattcgcta tcaataaaac gaactatata gcttggtgga tcagaaaaga caaagttgcc
    37981 gccgatgtat ttttcgcctg cttcaaagaa gtggataaat tcaggttgcc aatcagattc
    38041 ttcatgttgt aataaaagcg attgaggcgt ggcagtcaac tggataaaca ggctttgaca
    38101 acaggaattt ttgatgtcgt ttagcagttt attgatagta ctggcatctt tagccggctt
    38161 atcagaatta gtatttaggc tggcagcatc ggcttcatcg tccacaataa ccaaaggata
    38221 gcctttcagg ctactttgac tggcaaacaa attacgccaa cgtttcaata cacgggcatt
    38281 ctttttaatc acgaccaaga tggggttctc tgccttcatt acttccataa aacttcggtc
    38341 gtcagcttca gacaatacga taaagttttt cagattggct ttggctcgtt tgactgtttg
    38401 atcctgtaaa tctacgctgt cagtagtaag atacaaaaaa actttgtggt cgccatcatc
    38461 agccagcgca cttaatacac ccaaaacctg tgccgttttg ccactttgaa cattgcccag
    38521 caataaaaca ttttgggcga ttttcggctc ggtttgtgac aatttttcca taaaaccatc
    38581 aacggtattt ttaacagaat cagctaattc gggaggggtc aattggttca agtaggttcg
    38641 caacatttag tttttcccct taaatgagag cagccaaaca tcaggattat cggttgaacg
    38701 taactcaaaa tgatcgttac cgtattcgcg tagggtttca tgcgtaatct tctcgttatt
    38761 ttgcaggcag ccatgacttt ccaaacgacc tttaatccat ttaccaagtg tctttaaatc
    38821 attttctgag cggaagtttt tggagtaatc ccccgatgtc ttgcattgaa attgccaacc
    38881 atcatcggta ataactgtaa aagacttcag aaccggatac ccttcttgtg aagtgatgtc
    38941 tttggataca atcaactcta cttcatacca aggtcgtggt ttgacaaacc ctcgtttatc
    39001 tcgtcgacct tctccaaaaa atacattcaa attacttttt tcttcggttt tagctggaat
    39061 tgagaaatga tattctgacg tttgggcaaa taattgccga atctgttctg gtgcaatttt
    39121 ctgaacaccc aaacaatttt ctaaatggct attatgttca ataaatttac ttggtctttc
    39181 aacttcttgg atatttttgc ctagcttatg aatagtactt tgaatatctg cctgcaaaat
    39241 ctgtgcaggc ttaccgttca aatgcaacat agtttcataa gtacgttccg tgctatccca
    39301 aaaacacgtt aagttagcag aaccaattaa tccattgatt ttttgataat ttttaaagga
    39361 atacattttg ccgtgaaatt tcacaaaggg ggaaacatag acggctcctc ttttctcatg
    39421 tcgcaaaaaa tcatttagtt tacataagct gtcatattgt aaatggctaa aaccttccaa
    39481 ataatgcatt ccgaccaata aatctatttt ttgaatatga tcgtttagct ccaaaatttt
    39541 atgtaactca accactgcgt cattagatac ataacctgtc gccatcaaca cttcatccgc
    39601 tgatttaaat aaatccatcc aaaccgcatt aagagatttt tcagtgattt tggcattggc
    39661 gatattggaa aaaactgtat tcattgtttt ccccctcgaa ttttgcttag tcggtcatgg
    39721 aataaaattt ctttttcttc gactaatttc tcatgcaaat ctactttctc atagttgtct
    39781 gaaaaaatcg gtaaaatgct ttttgccagt tcaaccacgc cctgaggagg aacagcatta
    39841 ccaatctggc gacgtacttc agttgttgat ccgacaaact caaaatcatc aggaaaactt
    39901 tgaagccttg ctcgttctct attagtaaaa gcacgcggtt cagggaagtg atagccccaa
    39961 gtaccaccgc cacctgctgc aataattgtt tttgatggct cgttccgatg catacgacga
    40021 taaacgtggc taatcatacc tttcacatat aaaggatgat ctttaggaat atcggtaaaa
    40081 tttccacctt caggaattaa ttccaacata cggcgtgttt tatcgctgat tttcagtaat
    40141 tcattattac tggcattttg tggaatattg gatatggcct gacctgctgt tacatatggt
    40201 tttaagccgt tttcgccagt ttcattgtgc gtcggttccg gatggcgaaa atcaaatcct
    40261 gtatccaaac gtactccgac aatcagcaca cgttcacgaa attgaggtac gccaaattct
    40321 gcaaagttat acagcttcgc ctgaacgtaa taaccgcaat tttcaaagtc ggtaataatt
    40381 tgctggatgg cttttttctt gttggcagtc aataaacctt tcacattctc agcaacaaaa
    40441 actttcggtt tttttgcatt tacaaaacgt aaaaagcttt tataaagatt gccgcgctca
    40501 ccctctaagc ccggctgttt ccaaatcatg gaaaaatctt gacaagggaa tccgcctaaa
    40561 atgatgtcgc aatcgggaat agttggatca ttcggattaa tttgttcaat atcaccttct
    40621 acgatgacat cgccgatatt tttacggaaa ctttcgcaag cccaatggga gaaatcgttc
    40681 gcccaaacag tttcacaacc agcttgatga aagcccaaat ccaaaccgcc acatcctgaa
    40741 aatagagata ggatttttgg tttgttacta gaagaattaa gatttgagat ttgtttagat
    40801 agcataggat ttcactccta tgcataaatt tgataaaaat ccttgtatgg attttaaagg
    40861 tgaactatgc tct
//
LOCUS       DAXDZG010000006        40798 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXDZG010000006 DAXDZG010000000
VERSION     DAXDZG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40798
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.006"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(371..2976)
                     /gene="acnD"
                     /locus_tag="ACFB78_000282"
                     /pseudo
     CDS             complement(371..2976)
                     /gene="acnD"
                     /locus_tag="ACFB78_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            complement(3053..3841)
                     /locus_tag="ACFB78_000283"
     CDS             complement(3053..3841)
                     /locus_tag="ACFB78_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC1268366.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(3993..5141)
                     /gene="prpC"
                     /locus_tag="ACFB78_000284"
     CDS             complement(3993..5141)
                     /gene="prpC"
                     /locus_tag="ACFB78_000284"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC1268367.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5226..6101)
                     /gene="prpB"
                     /locus_tag="ACFB78_000285"
     CDS             complement(5226..6101)
                     /gene="prpB"
                     /locus_tag="ACFB78_000285"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC1268368.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKTIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            complement(6721..8031)
                     /locus_tag="ACFB78_000286"
     CDS             complement(6721..8031)
                     /locus_tag="ACFB78_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC1268369.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(8167..9345)
                     /gene="ftsZ"
                     /locus_tag="ACFB78_000287"
     CDS             complement(8167..9345)
                     /gene="ftsZ"
                     /locus_tag="ACFB78_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC1268370.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9464..10708)
                     /gene="ftsA"
                     /locus_tag="ACFB78_000288"
     CDS             complement(9464..10708)
                     /gene="ftsA"
                     /locus_tag="ACFB78_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC1268371.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(10794..11522)
                     /locus_tag="ACFB78_000289"
     CDS             complement(10794..11522)
                     /locus_tag="ACFB78_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC1268372.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(11512..12426)
                     /locus_tag="ACFB78_000290"
     CDS             complement(11512..12426)
                     /locus_tag="ACFB78_000290"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC1268373.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(12538..13947)
                     /gene="murC"
                     /locus_tag="ACFB78_000291"
     CDS             complement(12538..13947)
                     /gene="murC"
                     /locus_tag="ACFB78_000291"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC1268374.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(14102..15169)
                     /gene="murG"
                     /locus_tag="ACFB78_000292"
     CDS             complement(14102..15169)
                     /gene="murG"
                     /locus_tag="ACFB78_000292"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC1268375.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15173..16444)
                     /locus_tag="ACFB78_000293"
     CDS             complement(15173..16444)
                     /locus_tag="ACFB78_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC1268376.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRQKMRGYRVE"
     gene            complement(16623..17960)
                     /gene="murD"
                     /locus_tag="ACFB78_000294"
     CDS             complement(16623..17960)
                     /gene="murD"
                     /locus_tag="ACFB78_000294"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC1268377.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQSDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18045..18425)
                     /locus_tag="ACFB78_000295"
     CDS             complement(18045..18425)
                     /locus_tag="ACFB78_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC1268378.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(18550..19632)
                     /gene="mraY"
                     /locus_tag="ACFB78_000296"
     CDS             complement(18550..19632)
                     /gene="mraY"
                     /locus_tag="ACFB78_000296"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC1268379.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19877..20053)
                     /locus_tag="ACFB78_000297"
     CDS             complement(19877..20053)
                     /locus_tag="ACFB78_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268380.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(20056..21414)
                     /gene="murF"
                     /locus_tag="ACFB78_000298"
     CDS             complement(20056..21414)
                     /gene="murF"
                     /locus_tag="ACFB78_000298"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC1268381.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21539..23185)
                     /locus_tag="ACFB78_000299"
     CDS             complement(21539..23185)
                     /locus_tag="ACFB78_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC1268382.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(23208..24686)
                     /locus_tag="ACFB78_000300"
     CDS             complement(23208..24686)
                     /locus_tag="ACFB78_000300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC1268383.1"
                     /translation="MFSKLTPLAETNIPPLLCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVVNGAAFVFWDDDGKFAWNPEWKVPNQGIKNLKHRAGMLAAQVYDN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24711..26462)
                     /locus_tag="ACFB78_000301"
     CDS             complement(24711..26462)
                     /locus_tag="ACFB78_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC1268384.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTTRKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHSYYGGVVAGPPFKKIMGGSLNILG
                     VSPTKPLTNVAAVKTPS"
     gene            complement(26523..26786)
                     /gene="ftsL"
                     /locus_tag="ACFB78_000302"
     CDS             complement(26523..26786)
                     /gene="ftsL"
                     /locus_tag="ACFB78_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC1268385.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(26813..27769)
                     /gene="rsmH"
                     /locus_tag="ACFB78_000303"
     CDS             complement(26813..27769)
                     /gene="rsmH"
                     /locus_tag="ACFB78_000303"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC1268386.1"
                     /translation="MSGAESYRHITVLLNEVVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(27766..28221)
                     /gene="mraZ"
                     /locus_tag="ACFB78_000304"
     CDS             complement(27766..28221)
                     /gene="mraZ"
                     /locus_tag="ACFB78_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC1268387.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            28500..29747
                     /locus_tag="ACFB78_000305"
     CDS             28500..29747
                     /locus_tag="ACFB78_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1268388.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            29843..29919
                     /locus_tag="ACFB78_000306"
     tRNA            29843..29919
                     /locus_tag="ACFB78_000306"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29877..29879,aa:Met,seq:cat)
     gene            29927..30002
                     /locus_tag="ACFB78_000307"
     tRNA            29927..30002
                     /locus_tag="ACFB78_000307"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29960..29962,aa:Ala,seq:ggc)
     gene            complement(30188..31009)
                     /locus_tag="ACFB78_000308"
     CDS             complement(30188..31009)
                     /locus_tag="ACFB78_000308"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC1268389.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(31042..31686)
                     /locus_tag="ACFB78_000309"
     CDS             complement(31042..31686)
                     /locus_tag="ACFB78_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC1268390.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(31689..32552)
                     /gene="ftsN"
                     /locus_tag="ACFB78_000310"
     CDS             complement(31689..32552)
                     /gene="ftsN"
                     /locus_tag="ACFB78_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC1268391.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(32658..34154)
                     /locus_tag="ACFB78_000311"
     CDS             complement(32658..34154)
                     /locus_tag="ACFB78_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC1268392.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHGAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(34168..34488)
                     /locus_tag="ACFB78_000312"
     CDS             complement(34168..34488)
                     /locus_tag="ACFB78_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC1268393.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34976..36502
                     /gene="putP"
                     /locus_tag="ACFB78_000313"
     CDS             34976..36502
                     /gene="putP"
                     /locus_tag="ACFB78_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC1268394.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            36754..40359
                     /gene="putA"
                     /locus_tag="ACFB78_000314"
     CDS             36754..40359
                     /gene="putA"
                     /locus_tag="ACFB78_000314"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC1268395.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAESVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAAAGIRTVVEPGSPLASYSADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSVNTTAAGGNAGLMAVAD"
     gene            40630..40798
                     /locus_tag="ACFB78_000315"
                     /pseudo
     CDS             40630..40798
                     /locus_tag="ACFB78_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261433.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
BASE COUNT         9618 a        11554 c        10138 g         9488 t
ORIGIN      
        1 tttgttaatc cgctatacca tgtcaattca gcctttattt cgtgggcaaa gcccacgcta
       61 cacccacttt ccagaagtac attaggctac cactccgacc aaagccgttt gattccgcaa
      121 actgtcgggg ttcgccccaa tctacggctg ctgtgttgtg atacggtaga ttattaaacc
      181 ccgtcattcc cgcgtaggcg gaaatccaga tttatccgca cagaaactca tcggataaaa
      241 aggtttcctc aattccactt tctggattcc cgcctgcgcg ggaatgacga tttacagtaa
      301 atgcctgcca tatcgggaat tacgtgaatg agaacccgac ccacgctacg tcttgctttt
      361 cagacgaact ctaagccgcg ttcccttcca aaaagtcctg tgcaaaccgt tgcaacacgc
      421 cgccggcttc atataccaat acttcttctg cagtatcggg gcggcaggta accggaactt
      481 cgacggtttc tccgttttta cggtgaatca cgagggtcag gccgcagcgc ggtgtgcgtt
      541 cgccgacaac gtcgtaggtt tccgtaccgt ccagttgcag ggtatggcgg ttggtgccgg
      601 gtttgaattg cagcggcaag acgcccatgc cgatgaggtt ggtgcggtgg atgcgctcga
      661 aaccttcggc ggcgatggct tccacacccg ccagccgcac gcccttcgcc gcccggtcgc
      721 ggcttgagcc ttgaccatag tccgcaccgg caatgatgat aagcggctgt ttgcggttca
      781 tataggtttc gatggcttcc cacatgcgca tggtttggcc ttctggttca acgcgtgcca
      841 acgaaccttg gcgtacgctg ccgtcttcgt ttctcaccat ttcgttaaac agtttcggat
      901 tggcgaaggt tgcgcgttgg gcggtcaagt ggtcgccgcg gtgggttgcg taagagttga
      961 agtcttcttc aggcaaaccc attttcgcca aatattcgcc tgcggcactg ccggccaaaa
     1021 tcgcattgga tggcgagatg tggtcggtgg tgatgttgtc gggcaaaatc gccggcggac
     1081 gcatacctct taatgtacgt tcccctgcca gtgcgccttc ccaatagggc ggacggcgga
     1141 tgtaggtgga catcggtcgc cagtcgtaca gcgggcttgg tgctttttgc gctgtgccgg
     1201 tgtcggacat cgggatataa atgtcgcgga attgttgcgg tttcacatat tcggcaacca
     1261 tggcatcgat ttcttcgtct gtcggccaga tatctttcag gcggatttcg cggccgtctg
     1321 caacgccgag tacgtcgttt tcaatatcga aacggatgct acctgccaat gcgtaggcaa
     1381 cgaccaaagg aggcgaagcg aggaaagcct gtttcgcata cggatggata cggccgtcga
     1441 agttgcggtt gcctgacaat acggcggtgg cgtacaaatc gcggtcgatg atttcttgtt
     1501 ggattttcgg gtcgagcgcg ccgctcatgc cgttacaggt ggtacatgcg aaggcgacga
     1561 taccgaagcc gagtttttcc atttcgggca acaagcctgc ttctttcaaa tagattccgg
     1621 ctacttttga acccggggca aacgaagatt tcacccaagg tttgcgtttc aagccgaggc
     1681 ggttggcatt gcgtgccaac agtgcggcgg cgacaacgtt gcgcgggttg gaagtattgg
     1741 tacacgaagt aatcgcggca ataatcactg caccgtcagg catttggccg tctgaaggct
     1801 cttcgtaagg cttcgccagc cctttcgccg ccaaatcggc ggtggcaaaa cgcgcgtgcg
     1861 ggttgctcgg gcctgccata ttgcgcgtta cgctgctcaa atcaaatttc aaaacgcgcg
     1921 gataaacggc ggttttcaag ccacctgccc ataagcctgc ggttttggcg taggtttcca
     1981 ccaatttcac ctgcgcgtcg tcacgtccgg tcagtttcaa ataatcaata gtttgcgcgt
     2041 cgatggcgaa catggcggca gtcgcgccga actccggcgt catgttggaa atggtcgcgc
     2101 ggtcgccgat agacaggctt ctcgcgccct cgccgaaaaa ttcgacaaac gccccgacca
     2161 cgcgctcttt acgcaagaat tcggtgagtg ccaacacgat gtctgtggca gtaatgcccg
     2221 cctgccgttt gcccgtcagc tccacgccga caatatcggg caggcgcatc atggacgcgc
     2281 gtcccagcat cacggtttcc gcttccaaac cgcccacgcc acggaaatca cgcccagcgc
     2341 atcaacgtgc ggcgtatgcg agtccgtgcc gacgcaggta tcggggaaag ccacgccgtt
     2401 tttgacttgg acgacgggcg acattttttc cagattgatt tggtgcatga tgccgttgcc
     2461 cgccggaatc acgtccacat tttcaaatgc ggtttttgtc cagttgatga agtggaaacg
     2521 gtcttcgtta cgtctgtctt cgatttcgcg gtttttgcgg aaggcatcag gatcgtaacc
     2581 gccgcactcc accgccaaag agtggtcgac gataagctgc gtctgcacca ccggattcac
     2641 tttggcagga tcgccgcctt tttcggcaat cgcatcgcgc aagcctgcca aatccaccag
     2701 cgcggtctgc cccagaatat cgtggcacac cacccgcgcg ggataccacg gaaaatcgat
     2761 ttcctgcttc ccttctatca actggcccag ccagctttgc agcatcggca aatcgacttt
     2821 gtccgcgcgg ttgaccaagt tctcagacaa aatgcggctc gtgtacggca gcttgtcgta
     2881 agagccgggt ttgatgtcct cacacgccgc acgcgcgtcg tagtattcca aatccgtacc
     2941 gggcagcggt ttgcggtaac tttggttggc agtcatgtcg gttctcctgt ggatctattt
     3001 ttcttgtggt ttgggtttca gccgatgttt tgaaggagtc gtctgaaagg gtttaaaaca
     3061 tcgaaacaat catctgatac agcggatttc tctcgtctat caacaatttc acagccatcg
     3121 aaatgctgat gacaatcagc agcggcttaa tcagcttcga accgaagcgg acggcaaatc
     3181 tcgcacctaa attcgcaccg acaaacgcac cgaccgccat cgttgccaca atcgggaaaa
     3241 taatcgaacc gtgcagcagg aataccgata gcgaaccaag attgcaagca acgttcgcca
     3301 atttggtgta agacatcgcg ttcaacagct tgcagccgag caaaacaata aaggcaatca
     3361 gaaaaaacga gccgacaccc ggtccgaaca caccgtcgta aaaacccaaa agcggtgcaa
     3421 ccgtcagccc gaatagaaaa aaagacattc tggctttgcc ttccttactg ccgtcgagct
     3481 tgggcgaaaa cacaaaatac agcgcgacaa atatcaacaa aaccggcacg accgccagca
     3541 aaatatcttt ggaaaccaag ctgaccgata atgcaccgac cacgccgcct gcaaacgatg
     3601 ctgcggcaat cgggagactt ttcttccaat caatcaaacc tttgcgtgca aaagaaaccg
     3661 tagccgaaaa cgtagcagcg gctgcttgca gcttgttggt ggcaattgcc gacacgggag
     3721 gaatacctgc caacaagagt gcaggcagcg taatcaaacc acccccgccc gcaatcgcat
     3781 cgataaatcc ggcgatcatc gcaaccaaac ccaaagcgag tattatgtat aaatcttcca
     3841 tgtttcttat cctgttactt gcgccaatac aggattatct tctcctatta gattaaactt
     3901 atttcagaca acctttccaa taaggcaagg tcgtctgaaa tcctcagctt tgcataccga
     3961 aataaagaac aaactgctat tgcattcttc aattatcgtt cttcaatctc cacaaacgcc
     4021 aaatcttcag ggccggtgta gtttgcgctc ggacggatga ttttgccgtc tttgcgctgt
     4081 tcgaggacgt gtgcgctcca accggttgta cgggaaatca cgaacagcgg tgtgaacata
     4141 gcggtcggta cgcccaattt ttggtaggaa acggcagaga accagtccag attcgggaac
     4201 atttttttct cttcccacat cacgctctcc aagcgttcgg cgatgtcgaa gaggcgcatg
     4261 tcgcccgctt cccggctcaa accgcgcgcc acttctttaa tcacgacgtt gcgcgggtcg
     4321 gaaatggtgt aaaccggatg gccgaaaccg atcacgattt ctttgcggcc gatgcgttcg
     4381 cggatgtcgg cttcgtcggc gttgcggtag cgtttttgga tgtcgtaggc gacttcgttc
     4441 gcaccgccgt gtttcggacc tttaagcgcg ccgatggcgc cggtgatgca ggaatacata
     4501 tccgaacccg taccggcaat cacgcgggca gtaaaagtgg aggcgttgaa ctcgtgttcg
     4561 gcatacagaa tcagtgaaac atgcatggct ttgatgtgtg attcgcttgg gcgtttgccg
     4621 tgcaacaggt gcaggaaatg accgccgacg gtctcttcgt cgctttcaac ttcaatacgt
     4681 ttgccgttgt gcgaatattg ataccagtag agcaggatgc tgccgaggct ggcgatcagt
     4741 ttgtcggcga tgtcgcgcgc ttcgctgtcg ggatggcttt cgcgttcggg atgaacgcag
     4801 cccagcatgg atacgccggt acgcatcacg tccatcggat gggtatgtgc aggcagggtt
     4861 tccaaaactt taatcacacg gataggcagg ccgcgcatgg atttgagctt ggttttataa
     4921 gcggccagct cgaatttgtt gggcagatgg ccgtgaatca gcaagtgtgc gacttcttca
     4981 aactcgcatt tttgtgccaa atttagaatg tcgtaaccgc gatggctcaa atcgttaccg
     5041 gtacggccaa cggtacacaa agcggtatta ccggccgcaa cacctgaaag cgcaacggat
     5101 tttttaggtt tgaaggtcgg ggtttgagta gtttcagtca tggtatttct cctttgtatt
     5161 tttatgggtt ttatttttca gacggtcgat gcggatttgt tgaaaggccg tctgaaagcg
     5221 gtaaatcatt tttgaaacaa tttatccagt ttttgatcga aggcatgata gttcagatgc
     5281 tcgtacagct cggcacgggt ttgcatactg tccaccaccg ccgcctgagt gccatcgcgc
     5341 ataatcgctt cgtaaacatt caaagcggct ttgcttgctg cgcggaatga tgacaacgga
     5401 tacagcacca gtgacacgcc gttttcagcc aactcgcttt gcgtataaag cggtgtagca
     5461 ccaaactcgg taatgttggc caatactggt actttcactg catctgcaaa ttgacggtac
     5521 attttcaaat cggtcatggc ttcagggaaa atcatgtccg caccggcttc gacacaagcc
     5581 tgagcgcgtt cgatagcggc atccaaacct tctaccgcca gcgcatcggt acgcgccata
     5641 atcacgaagt tctcatcaac gcgcgcatct acggcagctt tgatgcggtc gaccatttca
     5701 tctttggaaa cgatggcttt gttcggacgg tggccgcagc gtttttgcgc tacctgatct
     5761 tcgatgtgaa ccgctgcaac accggcgcgt tcaaagttgc gaatggtacg ggcaatattg
     5821 aatgcaccgc cccaacccac atcgatgtcc accatcagag gcgtatccac gttgtccgta
     5881 atgcgtcgtg cgtcgatcag cacatcttcc attgtggtaa tgcccaaatc agggataccg
     5941 caagaacagg ctgccacgcc gccgccggac agatagatgg ttttgaaacc gctttgggtg
     6001 gccaatcgtg caaagtaagc attgacgcaa ccgacgacgg caagcggatt cgattctttc
     6061 acagcttggc ggaaacgtgc tccggcagag tgttgactca tcatatttct cctttataga
     6121 cttttttcag tatggacagg cttccatcac attcggacgg caaaacacag ccatccgacg
     6181 cgtcgggcat cccaatcatt aaaaatatat gggaaaaatt atcttattga tatttaaaac
     6241 gaatcaaaga aaaacagcag accgttcgga attatgcggc aaaaccgcag acaagaagaa
     6301 aacaagggga ttattcagaa aagggaaaaa catctgaatt ggtttcatag taatgttcct
     6361 ttgtagtatt atgtagtttt atttttcggt aatctaaaac acaagactgg aatgtcaaac
     6421 atttttaagg agactaatta cataatttta ttattttatt ttaacattat gattaatata
     6481 aattaatttt aacttatttg tttccgataa aattaatagg aaatttagaa aaattttcaa
     6541 taatcaaaat cctgaaaaat tataaaatat tttatatata aaacaatgaa ttattttcaa
     6601 gtaatccgat gtagtttatt atctatttta tttttatatt atttatttaa aattaaatat
     6661 atctggcaaa caaaatgccg tctgaaaacc tttcagacgg catttttaat atcgaatcaa
     6721 tcagcccaaa taccagaatt tcaatgccca caatacggca acaacccata ccataggcgg
     6781 cacgtcccca gtccggcgac acaaaagttt gaccacggca tagctgatga agccgaaggc
     6841 gatgccgtct gcaatcgaat aggtaaacgg catgaagaca atggtcagga acgcgggcgc
     6901 ggcttcagtc atatcgtccc aatcaatgtc cctcgcactg cggagcatct gcgtgccgac
     6961 ataaagcagt gcgggcgcgg tggcaaatac cggaacactt ttcgccaatg gggagaacat
     7021 cagacacgcc agcatcaata cgccgacggt aaccgccgtc aggccggtcc gtccgcctgc
     7081 cgatacgccc gccgcgcttt ccacataagg cgtggttgaa gaagtaccca aagccgcacc
     7141 tgccacaatg gcggtagagt ctgcaagcag tgcgcgtttc aggcggggca gcttaccgtc
     7201 caccagcagt ccggcacggt gggatacgcc gaccagcgtt ccggtactgt caaacaaatc
     7261 gaccaagaag aagacgaaaa tcacgctgac catgctgacg gtaaacagac ctttaaaatc
     7321 catctgcata aaggtcggcg caatgcccgg tacttcgccg accacgccgt gaaactcgtt
     7381 caaacccatc aggctggcaa tgacggtaat cgtcagaatg gtgatgatga ttgcgccttg
     7441 aacgcggaaa taccccaata cgaccaccat gacaaaaccg aacaatgcca acagtgcgct
     7501 gggctgatga atatcgccca agccgaccaa ggttgccgga ttggcaacga taatgcctgc
     7561 gcctttcagg gaaatcagtg ccaaaaacaa accgataccg gcggcaatcg acattttcaa
     7621 acccataggc agtgcgttga ccagcatttc cctgacttta aaaaagctga acaggatgaa
     7681 aatcagaccg gaaatgaaca ccgcacccaa cgccacctgc caaggcacgc ccataccctt
     7741 aacgacggca aaggtgaaat aggcattcag ccccatcccc ggggcaagcg caatcggata
     7801 gttgccgata aaacccatga caaaacagcc gatggcggat gcgatacagg tggcgacgaa
     7861 taccgccccc atatccattc cggtctcgcc caaaatcagg ggattgacga taacgatgta
     7921 gcacatcgtc aaaaaggtcg tcaaacccgc catcaactcg gtacgtaccg tcgtaccgtt
     7981 tgccttcagg ttaaaaaccc tatccagcaa tgtttgtttt gaaatatcca tatctagcag
     8041 tccgtttcaa gtaaaaaatc tgaacccgca caccgcaaac cgcacaggat tcggcatatt
     8101 gaaaaatata cctcccaaat cgggaaaacc ggttcgggag gcaataagtt tacggcagac
     8161 ggcacattat ttgtctgaat tgtgttgacg acgcaaaatt gcaggaattt caaaatcgtc
     8221 aaggacagac tggttatcga aatccgctgc tgtcagattc atggtgcgga taccgcgatt
     8281 ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacgggg caaccgcttc
     8341 tacttccctt gccggagtag gatcgaccgc tcctttttct ttcagaccgg tagcgataat
     8401 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gcggcaccga atttgcattc
     8461 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcagacaact cggacatttt
     8521 caggcaaccc ggagcagtcg taatattgac cagcacaccg cgtgcaccat ccaaagttac
     8581 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     8641 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     8701 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     8761 cgcatcgcgc aatacattgt ccgccgcgcg gaaggcttcg cgcatagtta cgtcttcacc
     8821 caatgcggtc atcagtttgt cgttcgggat gataatcagc gaatcgacgt gttctttcaa
     8881 ctgttccaac cctgcctgtg ccacatggac gcgtttgcct tcataagaaa acggacgggt
     8941 aactacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     9001 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattgg caccgcgaat
     9061 ggcttcttca atggcctccc gatcttcctg tgctgccgca cggccgatat cgggattcgc
     9121 acccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     9181 ttttgccaaa gactgcgcat ctgtattggc actgataaac tccacactgc gcacattgtt
     9241 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaattac
     9301 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgat
     9361 cgccccgttc agaggacggt ttaaataaat tattatttat tatataagaa gtatcttact
     9421 gctggcaaaa tacttctcac ctgtcaaacg gcaatccaac tgttcagagg ttgttttcaa
     9481 tccaccgctt caatcttgcc aacaaaccgc cgcccccttc tctctcttgc actgtaccgt
     9541 tttccggttg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatagcgg
     9601 tagaaaagcg cggtgtgcgg acacggtcgg acaaaccgcc catttcttgg ggcgcaccgg
     9661 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     9721 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     9781 gttcgcccag cactacgcca aaaatctcct gaatacgtgc gctgatgatt gctgccaaaa
     9841 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcgc
     9901 ccaagccttc cgtatcgcat gatgccacgc cataatggat tttaatgtac tcggcggcat
     9961 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
    10021 tgacggacgt atggcggatg gcgccgttca tataaacggc aatatcggtc gttccgccac
    10081 caatgtcaat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
    10141 ttgccaacgg ttgaagcatg atctgatcgc ttttcaagcc gcacagctcg atacattttt
    10201 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccagacgca
    10261 caccgctcat accgatgggc tcccttacac caagttgggt gtcgataatg tagtcttgaa
    10321 ccacggcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
    10381 cgcggtcgat gtctgcctgt gttacttctc cgtctttgat tttaaccaca ccttgcgaat
    10441 taaggctgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
    10501 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
    10561 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgatg atgttgattt
    10621 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
    10681 cgctgatgta tcttttctgc tgttccattg ttcgtctgcc cttaaaactg attgaaattt
    10741 gcgtcgcacc gtttcagacg gcacggccgt aatatgtccg atacctgttc ccactattct
    10801 tcggattctt tttcgggtaa accgtcggga gcatggcgga ctgaaaatcc gtccttatac
    10861 ctcatatcca cataggataa ccgattttta ttcttacgca acagatgctg ccacgcttcg
    10921 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
    10981 tccaaaacga cattccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
    11041 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
    11101 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
    11161 tcgccgtcca ccaaggcatg gtcgccccaa cgtgcaaccg gcttgcgctc ggtcaggacg
    11221 acctcaaccg tatcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
    11281 cggtaggctt cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
    11341 ttcgccaaac tgcccaatgc cttcttatcg gaataaacca ggttgccctt cagcgacacc
    11401 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
    11461 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
    11521 atgtgcggcc ttcaaaattt caatacataa atcggcaaaa cccacgcccg taacggcagc
    11581 ggatttcggt actaaactat ggccggtcat accgggcagg gtgttgattt ccaacagata
    11641 gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagccttccg caccgattgc
    11701 ctgtgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcaaatcttc
    11761 cgaaggacat tgataaatgg tgtcgtctcg gttgtacttg gcttcgtagt cgtaaaactc
    11821 ggttgcgggg atgatgtgta tgccgggcag ccctttgccg ttcaggacgg ggcaggaata
    11881 ttcgccgccg ccgataaaac gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
    11941 gtaaacgctt ttcagacggc ctttttcttt gacttttacc acgccgacgc tgctgccttc
    12001 ggccgccggc ttcacaaaca tcggcagacc caatttttct tcgacggcat cgaaatcggt
    12061 atcatcgtac agtacggcga actcgggaac gggtaatccc aatgcctgcc aaatcagttt
    12121 gcagcggtat ttgtccatgc cgatggcgga ggcggcgaca ccgctgccgg tatagggaat
    12181 gcccaacagt tccaatgcac cctgaacagc cccgtcttcg ccgtaagtac cgtgaaggat
    12241 gttgaatgcc gtctgaaagc cccgctcctt cagttcggat aacggcgttt ccttagggtc
    12301 gaaggcgtat gcgtctatgc ctttgctttt taaggcgttc aaaatggcgg taccgctgtc
    12361 cagcgagatt tctcgttcgc tggaaaaacc acccatcaat acggccactt tgccaaaatt
    12421 ctgcattgtt tttgttcttt cctaattgct ttatgcttgt tgtcagaggt cgtctgaaac
    12481 ctgattgcgg tttcagacga cctttatatg atgttccgtc tgtcaggcgg gtgtatctca
    12541 aatctgtttc gacaattcca acagcgcgga gggaacgcgg ttgatgcttc ccgcacccat
    12601 attcaacaca acatcgccat cctgtaaaac attcatcagc atttgcggca ggtcggcgac
    12661 gttttcgcag taaatcggct caagtttgcc caatacgcgg atagcacgcg ccaaggcgcg
    12721 ggagtcggcg gcggcaaccg gctcttcgcc ggcggcataa acttcggtca gtaccagcgc
    12781 atcaacggta ttgagtactt tggtaaagtc ttcaaacaaa tcgcgcgtgc gggtatagcg
    12841 gtgcggctgg aaggcgagta ccaaacgttt ttccggatac gcgccgcgtg cagcggcaag
    12901 ggttgccgcc atttcgacgg ggtggtgtcc gtaatcgtcc accagcaaag cggtcccgcc
    12961 gtttggcaac ttgatgtcgc cgtatttttg gaagcggcgg ccgacgcctt caaagccgag
    13021 caagcctttt tggatcgctt caaccgatgc gccgacttcc agcgccacgc cgatggctgc
    13081 caatgcgttc agcacgttgt gtctgccggg catattcagc acgacttcaa acgacccctg
    13141 ctcatgtcct ttcatttgaa catggacggt gaatttcatt tgcgcgccga cgttttcgat
    13201 gtcggtggcg tagatgtcgg cggtatcgtc caaaccgtaa gtagcataag gtttgctcac
    13261 tttgggcaaa atcgcgcgga cgtgttcgct gtcaacacac aaaaaggctt tgccgtagaa
    13321 gggcatacgg tggatgaaat cgataaacgc ctgatgcagt ttttcgacgc tgtgcccgta
    13381 ggtatccata tggtcttcgt cgatattggt aacgacggac ataatcgggg tcagatgtag
    13441 gaaagaggca tcggattcgt cggcttcggc aacgatgtat tcgcctttgc ccaagcgggc
    13501 gttggtgcct gcggcgttga gtttgccgcc gataacgaaa gtggggtcga gtcctgccgc
    13561 gccgaggatg gaggcggtca ggctggtggt cgtggttttg ccgtgcgtac cggcaatggc
    13621 gatgccgtca cggaagcgca tcagctctgc cagcatcaag gcgcgcggaa taacgggaat
    13681 ttgccgctcc aacgcagcga caacttcggg attttctttc ttgacggcgg tagaggcaac
    13741 gacgacatcc gcaccgttaa cgtgttctgc ggtatggccg ggataaactt gaatgcccag
    13801 gctgctcaaa tgctcggtag cggcatttcg cgcctgatcc gaaccggaaa ctttaaagcc
    13861 caaattgtgc aagacttcgg cgataccgct catgccgacg ccgccgatac cgacaaaatg
    13921 gatgttgctt actcgatttt tcatcataat gttgcgttcc ggttggtttt tagtgcgtaa
    13981 aggcgttatt ttaaaggtct gaccgtttgc gcaccatagt tttctgacaa atatatagcg
    14041 gattgaaata aaaaacatcc atgccgtctg aacggctttt cagacggcat ggttcggcag
    14101 tttacgccgc acacgcaatc gcggcttcgg caacgtcatc cgcgctgtgc ggcaatgcca
    14161 acgtacgggc gttttccgcc catttgaggc atttttcgcg gttgaggctg ccgaggattt
    14221 cggcgagttt ttccgccgtc aactgggttt gcggcaacag cagccccgct tctgcctgca
    14281 ccatgaaacg cgcgttggcg gtttgatggt catcaacggc gtgaggatac ggcactaaca
    14341 acgcgcccag ccccgccgcc gtcaactcgg caatcgtcag cgcgccggca cggcaaatca
    14401 ccaaatcggc atcacggtag gcggacacca tgtcggtaat aaattccacg cattccgctt
    14461 tcacgcccaa cgcgtcataa tccgcctgaa gattgcccag cttgttacgc cccgactggt
    14521 ggtacatctg cgggcgcacc tcttcaggca gcagtgccaa cgcctgcggt acggttttgt
    14581 tcaaaacgtc cgcacccaaa ctgccgccga ccaccaaaat tttcagacgg ccttcgcgcc
    14641 cttggaagcg ttcggcaggc acgggcaggt tgctaatatc ggcgcggacg gggttgccga
    14701 ccaaaccgcc ttcgtggctg aacgctttcg gaaaagcgta caacacccgt ttcgcccagc
    14761 gcgacaggtg gcggttggac aagcctgcca cggcgttttg ctcgtgaatc acaatcggca
    14821 cgcccaagag tttcgccgcc agaccgccgg gaaaggtaac aaaaccgccg aagccgatga
    14881 cgcactcgac acggtgtttg cggataatcc gctgcgcttc gcggacggtt ttgtacagag
    14941 taaacggaag catcagcttg cgtttgatgc cgttgccgcg tattccttta atcgccagcg
    15001 tttccaagcg tatgccgtat tgcggcacga tgcgctcttc catcgaatcc ttgctgccca
    15061 gccaaattac atgatgaccg cgcacgcgca atgaatccgc cacagccaga gctgggaaaa
    15121 tgtgtccgcc cgttccgccc gccatcagca taaaggtttt accgcccatg atttactcca
    15181 cccggtaacc gcgcattttc tggcggtttt cataatcgat acgcaacagc agcatcatgc
    15241 tgatcagcat gaaaaagact gacgaaccgc cataggacat caacggcagc gtcagacctt
    15301 tggtcggcaa agcaccgatg ttcacaccga tattgaagaa actttggata ccgatccaaa
    15361 tgccgatacc cgaagcgata taggcgttga aagtcaaacc caaatcgcgc gactgcttgc
    15421 cgatggaaaa cgcccgcacc accagccagc cgtaacagaa tatcagcacg cacatcccga
    15481 agaagccgaa ttcttcagcg atgatggcaa aaataaaatc ggtatgcgct tccggcagaa
    15541 agccgcgttt gctcaaactc gcacccaaac ccataccgaa ccactctccg cgcccgattg
    15601 ccatcagaga gtgggtaagc tggtagccgg caccctgcgg gtctttccac gggtccaaaa
    15661 atgccactac ccgctgcaca cggtagggag cggcggtaat catcagcacc atcccaccca
    15721 agacgctgcc taccaggaca aaaaaatatt tccacggcaa tcctgccaga aacagcattc
    15781 caacggtaat gacggtaatg acgacaaacg aaccgaaatc cggctgtacc attatcagca
    15841 ccaaaccgaa cgccaccagc ataatcggca ggatgatcgc ccggaaacgg ccgtacattt
    15901 ctaatgtttc acgacgtgcc tgcggattgg tggcggacat aatcaggttg gccgtccccc
    15961 gccaaatcga ctgccaaccc aaactttcca tgctgcgcaa cacttcttca cggcgcgtga
    16021 acaggcttgc caaataaagg atgactgcca gcttgaacag ctcggtcggc tggaaattca
    16081 acggacccaa aggtatccaa cgggtcgcgc cattgatttc gcgcccggca atcaatacgg
    16141 ctaccagcaa caggccggat aaggcaaaaa tccacggcac aagccgccgc catgtcctca
    16201 tcctgcaaag aaaccataaa aaaccgctcg ctataaggcc ggcaacgacg aaccccgcct
    16261 gcctggtcaa atagaaaaac tgatcgccgc cttccttcga tgccaaatac acagaagccg
    16321 aataaatcat cagcaggccg aacgccgtca tcagcaccac catccacaaa agcggcgcgt
    16381 cgaatttcct gccgtcgcgc acaatcggcc tgtcgagcag cagagtgtgg acaccgtcgc
    16441 ccacttttac caatacttcc gaaatcttca aaaaaccacc tgccagtctg tttgcacctg
    16501 ccgcaaaggg caaaaatttc agacggcaga caatgccgtc cgaacatacg atacatccca
    16561 aatcggtatt ctaaatcttt acttgccgcc caacaatgac ggcgtttgtg tttcggacgg
    16621 catcacaaag ccttaaacgc ttcgataaac acttccgaac ggtgcgcgta gcctttaaac
    16681 atatcgaaac tcgcgcaggc ggggctgagc aagacaatat cgcccgcttc ggcttgggcg
    16741 tatgccgtct gaaccgcctc ttccaaagtg acgcagtcgg tcaggttcaa gccgcagccg
    16801 tccaaatcgc ggcggatttg cggcgcatcg acgccgatca ggaacacgcc ttttgcctta
    16861 tctttcaacg cgtcgcgcag gggcgtgaag tcctgccctt tgcccatgcc gcccaaaatc
    16921 acgaagagcg ggttttgcaa accggcaatc gcggcggcgg tcgcgccgac attcgtgcct
    16981 ttgctgtcgt cgatgaatac cacgccgttt ttctcgccga ttttttccac acggtgcggc
    17041 aagccttgga aggttttgac gtgttccagc aatgcttcgc gcggcaaacc gacggcctcg
    17101 cacaaagcca cggcagccat aacgttggcg gcgttgtgca aaccttgcag cgggatgtct
    17161 tgcgtagaaa tcaaatcttc attgccttgt tttaaacagc ccgtcccgcg ttccaaccaa
    17221 aaatcggctt cgtgttccaa ggaaaaccgt ttcacttcac gccctgcccg tttcatggcg
    17281 cggcagaaca cgtcgtccgc attcaaaacc tgcactccat cgccacggaa aatcttggct
    17341 ttggtatgcg cgtagtcgag caaatcgtcg tagcggtcga gatggtcttc ggaaatgttc
    17401 atcaccgtcg ccgcagtcgg gcgcaggctt tcggtgtttt ccagttggaa gctggaaagc
    17461 tccaacaccc acacgtccgc ctttttgcct tcgcgctgca attctgcctc caaaaccggc
    17521 gtaccgatat tgcccgcaat gacggtatcc agcccgcact tgatgcagag atagccgacc
    17581 aggctcgtta ccgtggtttt gccgttgctg ccggtaatcg caatcacctt gtcgccgcgg
    17641 cggttcacaa tgtccgccag caattcgatg tcgcctagca cgcgcccgcc gttttgcttg
    17701 aacgcctcga tatccgactg ccgctcgctg atgccgggac tgagcgccag aatatcgaaa
    17761 ccgttgtcca acgcatcttt cagacgaccc gtgtaaaaca ccagcccgtc aaacatctta
    17821 ccgatttgcg ccacgcgctc ggctttcagc tccgcatcat aagcggcaac ctccgcgccg
    17881 tttttgcgca gataggcaat catggaaata cccgtgccgc caagtccggc gacgaggatt
    17941 tttttgtttt ggaaagtcat tttggttttg tcctaaaaca aatcatattg ggcaggagac
    18001 gtccgccctt gcccaagccg ctttcagacg gcatcgcgag ccgattaata acccgccttc
    18061 aggcgttggt cattgtcgca gctgttttgg tctccgtttt gacaagcctt gccaagccat
    18121 tgttgagcga gcgcaaggtc ttggcgcacg ccgcgtccat cgtaatacat caagcccaaa
    18181 ttgtattggg cttgggcatc cccttgttct gaagccttgc gataccactg cactgcctgt
    18241 acataatctt gacgaactcc ttgtccattt tcatacatca cgcccaaatt gaattgggct
    18301 gctgcatttc cctgttctgc cgcctgcaag ttttcccgaa aatccgatac gtcacccgcc
    18361 cacaccgctt ggttcaagcc caaggcaatc agggcggcgg caagccattt gactgtctgt
    18421 ttcatggttt tttcctgttt tagtataagg cgggtttcag ccgccgttaa cgataggacg
    18481 gggcggattg ccgccgcagg tttattgcgc gttcaaatgc cgtctgaaag gtgttcggac
    18541 ggcataggtt cagcgaattt tgagggtact caaaccgatc aataccaaga cgatggtaat
    18601 aatccagaaa cggacgacga cttgggtttc tttccagcct ttttgttcgt aatggtgatg
    18661 aatcggcgcc atcaggaaga tgcgtttttt ggttttcttg taccagccga cctgaagcat
    18721 aacggacacg gcttctacga cgaacagacc gcccataatg acgaggacaa attcttggcg
    18781 gacgatgacg gcaacggtac cgagcgcggc acccaatgcc agcgcgccga catcgcccat
    18841 aaagacttgc gcgggatagg cgttgaacca caaaaatccg aggcacgcgc cgcacatggc
    18901 ggtgcagaat atagcgactt cgttcgcgcc ggcgacatag ggaagctgga ggtattggga
    18961 aaattggtag tgtccgctga cgtaggcgaa aatggcgagc ccggcggcaa cgaggacgac
    19021 ggggaaggcg gcaaggccgt ccaagccgtc ggtgaggttg acggcgttgg atgtgccgac
    19081 gatggtcagg taagacaaca ccaaaaagcc gaccacgccc agcggcaggg cgatttgttt
    19141 gaaaaacggg acaatcaaaa tattgttggc ggaattggcg gcaaggtaaa acaatgccaa
    19201 accggcgata acggcaacgc ttgactgcca caccattttg aatttggcgg acacgccgtt
    19261 ggggtcttta taaacgactt tgcgccagtc gtcgtaaaaa ccgagcgcac cggtggcaag
    19321 cagtacgccc aagagaatcc agatatacgg gttcgcccag ttgccccaca acagggtgga
    19381 cacggtaatg gcggtcagaa tcagcgaacc gcccatcgtc ggcgtgccgt ttttgacgag
    19441 gtgggtttgc gggccgtcgg tacgcactgc ctgcccgcat ttgagcgcgg tcagcctgcg
    19501 tatcgtccac gggccgaaca tcagggaaaa cgccaaggcg gtcaacgccg ccataacggc
    19561 gcggaatgtg gtgtattgaa aaatattcag accggttaac cagttgctga aatgtgcgag
    19621 ccataaaaac atggggcttc cttttttgtt ttgtcgttat agcggattaa caaaaaccgg
    19681 tacgtcgttg ccccgccccg gctcaaaggg aacggttccc taaggcgccc aagcaccggg
    19741 cggatcggtt ccgtactatc cgtactgtct gcggcccgcc gccttgtcct gatttttgtt
    19801 aatccgctat acatcgggct tgcgcccgct tgaggtttgc ttgctgtccg gcaaggtttc
    19861 ggacggcttt tgcagattaa cgtgcaggga ctttcccaat cacgcaaatc gggtagctcc
    19921 ccggattttt gccgccgccc aagtctaaac ataaatcttc ataaagatac gcctgcgtcc
    19981 gcattccggc ataaaacgcg ccgaccagcg cggcaatcag caataaggct ttaaaaaaac
    20041 gtcggctttt cattttcact tatcctccaa tgcctcgacc acttcttcca tctgcataaa
    20101 gcgcgaacct ttcaccaaca cggtggcgcg ttcgggcaaa tcgtggctca acacttgaat
    20161 caacgggtct ttggcggcga accacaaacc gtccgcgcca aatttttccg ccgcttcgac
    20221 gctgttgtcg ccgacaaaat aagccgcttc gatgccttgg tcgcgggcgt acgcgccgac
    20281 ttcggcgtgc atggcggcgg cttcgtcctc gcccagttcg cccatatcgc ccatcacgaa
    20341 aatgcgcggc gcaggcatac gcgccaacac gtcaaccgcg gctttcatac tgtcgggatt
    20401 cgcattataa gtatcgtcaa tcagggttgc gcccttgatg ccggctttga cgttcagacg
    20461 gcctttgatg ttgctgaagc cttgcaaacc ttccgccaca tcgttcaaac tcaaaccggc
    20521 agccaaagcc agcgcggcgg cagcggcggc gttgtggaca ttgtggcggc cgggaacagg
    20581 cagcaccacg gcagtgcgct cgtcgccgca caccaaatca aattcgcacg acaaaggttt
    20641 cagcacgata ttttccgcgc ggacatcgcc gctatcgacg ccgaaagtgc acgtattcaa
    20701 attaaacgtt gccgttttga agacagccat attggcatct tcttgaggaa tcagtgccat
    20761 gccgtctgaa cataagcctg cataaatctc gcttttcgct ttggcaatat cgcccactcc
    20821 gtcgaaaccg catccgacat gggcgcgcag ggcgttgttg accaaagcgg catcgggttt
    20881 ggcgatttgc gttaaaaccg ccagttcgcc aaaatggttc atgcccattt caatcacggc
    20941 atagcggtgt ttttcgttta atttcaataa agtcagcggc aatccgatgt ggttgttgaa
    21001 gttgcctgcc gtcgccgaaa cggcatcatc gccgaaacgg cggcgcaata ccgcagccag
    21061 catctccttc accgtcgtct tgccgcccga accggtaatg ccgaacacaa acgggttcac
    21121 attatcgcgc cacgccttcg ccaacgtttg caacgcggca agcgtgtcat cgactttcaa
    21181 cgcgccgccc aaagccgcgc aatcttcgcg cgaaaccaca accgccgccg cgcccgcaga
    21241 caatacgcct ccaacaaaat catgcgcgtc aaaccgcccg cccgccaatg cgaaaaacac
    21301 atcgccttcc cgaatatcgc ggctgtcggt tacgatgcgc gacacgggtt tgttttcaga
    21361 cggcatcgga agcttgaggg cttggcagat gaaatttagg tccagtggtt tcatatttgc
    21421 tttcgttaat attcgggcgg cggacacatc ggtagcggct gattttttta tcgcctgttt
    21481 tgctgtggta aaacacagat tattttccca ttctcattcg gcattttttc tgtacgtatc
    21541 attttttaga cgtattttta gccgatttgc cttttcccgc ataccacggc gcggggtcgt
    21601 cggactgtct gtcgataaag gcaaggttat tgccttcgcc cggcacatcg gggacattcc
    21661 cccaaaaatc atagccgtca tcgggcaact cgtcggtttc gatacccgtc caactgccga
    21721 atccgcgtaa aaaattaaac gcgctgcgcc tcactttaat catttcgcgc cgcgtgtcat
    21781 cggacgaaat tttaaccagc ggcacgccgt agctttgacg cttccactcg ccgtgtgtca
    21841 acgttcgctc gccgcctttg ccgacatgca tcaaaccgtg gtcggaaaag taaaccaaag
    21901 accagctttc tttattttca ttcaatatct taaccgtatc ttctaaaaat ttatcggttt
    21961 gcgcgatggt ggaaacatag caggatattt tttcagtttg atactgaaac cgccgcgcat
    22021 ccttatccaa acgtgtgcaa aaatcactgt gcgaacccat caggtgcatc acaatcagcc
    22081 gaggcttcgt gcctgcattt cctgtcaaaa cccgtttgaa cgccggcaac aaaaggcggt
    22141 cgctcaaccc cgcgcttttg ccataatcac ccctttgggt aaaccacgga taatcgctgc
    22201 gtagggcata ggtggaaatt tcgttggcaa aatgccccaa cattccttga ttagacagcc
    22261 acgccgtccg aaaacccgcc tgcttcgcca agctgacaat gttattgttc ggctctcccg
    22321 gcagccccaa agtctgcggc agcgaaagat tcgtcgcgtg ggcggtcgat tggtaaccgt
    22381 ttatcaacag ccctttggtc cgactcaaaa aaggcgtatc gggcaatggg aaaccgtaaa
    22441 cattcatata atccgaacgc gcgctctcac cgataaccac aacataattt ttatacttgg
    22501 gcgcaacatg acggatatgc catgttgacg ctttttttgc tgcttccaaa atgtgggcgc
    22561 gcttggcggc atattgtgcc ggcgcggaag ccaaatcgta atacaggtcg aaaatattca
    22621 acaacaggcc ggcatcaggt tctcgccaat ctttatcgcc ggcgattttc tccatcaccg
    22681 cgcaggataa aatcaaagtc aataatatgg tcagccatat tttgctgcgg cgtttatagt
    22741 tttttacgtc agcaaatacc cccacagata cacaatattt ccaaactgtc aagccaaaaa
    22801 tgaaaaataa tgcctgcaca aaataaagcg acccgggaag attgccgaca aattcacgcg
    22861 cctcggcagg attgctttcc aatatcgaac cgactatctg ataagaaggc gcaccataca
    22921 gccagccgac cggcaaatat agggcggttg tgccgacgta aatcagtaaa acaactgaag
    22981 aaacgcgcgg gaagccgcgt gccaataata ataaaaatac aaccgaagcc aaagcgccca
    23041 cggcagaata acggtagccg tattcatatt ccaagtgata acctgatgcg atggccgcgc
    23101 caaaacaaaa ggctaaaagc gcgcaaatgc ccaatgtatt ttttaatgtc tgatccatat
    23161 tttttattcg ggcggattgt ttcatacgat gccgtccgaa tatcccatta tttacgagtt
    23221 aacaaagcct gcccgacgat ttcaagatcg gaaaaacggt gcttcacgcc ctgtacatcc
    23281 tgatagtttt cgtgtccttt gccggcaatc aggatgatgt cgtttgcggc ggcctgctca
    23341 accgcataac ggatggcggc ggcacggtcg gcttcgacgc attcgggcgc gggcacggcg
    23401 ggcaggatgt cgttgatgat gtcttgcgga ttttccaaac gcgggttgtc gctggttacg
    23461 acgactttat ccgcgccctg tacggctgcc gcgcccatca atgggcgttt gccgcaatcg
    23521 cggttgccgc cgcaaccgaa tacgcaccat aaggccgcac cctgcggttt gatttcctgc
    23581 aaagtggaga gtgctttttc caatgcgtcg ggcgtgtggg cgtaatcgac gacgaccaag
    23641 ggcttgccgc tgttcatgat gcagtccatg cgccctgaag cgggacggat ttttgccagc
    23701 acatccaata ccttatcaag cggataaccg ttggcacaaa gcaaggcgat gcaggcggcg
    23761 aggttttgcg cgttgaaccg tccgagcagg cgggtgcggc attttccttc gccccacggg
    23821 gtttggaata ctgcttccat gccgtctgaa gaggcggtaa agtcggtaat gcggatgtcg
    23881 gcgtgttcgc tgaaaccata gctgtaaacg gctaaatcgg gacagtcttt tttcagacga
    23941 cctgcgagtt ccgcgccgta tccgtcatcg gtattgataa cggcgtgttt caagccgtgc
    24001 cagtaaaaca ggcgcgactt gatagcaccg taggcttcca tcgtgccgtg atagtcgagg
    24061 tggtctcggg tgaggttggt aaagattgcg ctgcggaatg gcacgccgtt gacgcgcgac
    24121 tggtcaagac cgtggctgga gacttccatc gcggcggctg ttgcgccctg ttggcggaaa
    24181 cggtagagca gggtttggac atcgacgggg gcgggggtgg tgtgcgtggt ttcttccaat
    24241 gcgccccaaa agccgttgcc gaccgtgccg ataatggcgg ttttttcgcc caataaatcg
    24301 gcagcctgcg ccagccattg tgtgatggag gttttgccgt tggtgccggt tacgccccag
    24361 actttgaggc cgtctgaaac gttgtcgtac acttgcgccg ccaacatgcc ggcacggtgt
    24421 ttcaaatttt tgatgccttg attggggact ttccattcgg gattccacgc gaatttgccg
    24481 tcgtcgtccc aaaaaacaaa agccgcgccg ttgacaacgg cggcggggat ataactgcgg
    24541 ccgtcggtat attcgccctg acaggcaacg aaaatatcac cttgtttgat ttgacggctg
    24601 tctgaatgca acaaacgccc tgctgcgttt gcacacagca gaggcggaat gttggtttca
    24661 gctaaagggg ttaacttgct gaacataaaa taatctcgtt gatactcgga ttaagacggt
    24721 gttttgacgg ctgcaacatt ggtcaaaggt ttggtcgggg aaacgcccaa gatgttcagg
    24781 ctgccgccca taattttttt gaagggcggc cctgccacta cgccgccgta atagctgtgg
    24841 gcagtcggtt cgtcgatggt taccgccaca atcacacggg ggtttttggc gggggcaaaa
    24901 ccgataaacg taccgacgtg tttgttgtcc acatagcggc cattgaccag tttgcgcgtc
    24961 gtaccggttt tagcgccgac atcgaaaccg tccaccgcac ccgccgtacc ggtgccgccc
    25021 ggctcggtta cggaaaccat cagattgcgt acctcgcgcg cggtcgattc tttgaatatg
    25081 cgtttgcctt gcggcgcaac cgcctgcttc tcaaagctga gcggcagcaa aacgccgtcg
    25141 tgcgtcagtg cggtataggc gcgcgccaat tgcagcaggc tcaattgcag gccgtaaccg
    25201 aaagacatcg tcgcctgttc gatgggccgc cacctgcgcc aatttctcaa caaacctgca
    25261 gtttcccccg gaaagcccga gtgcatacgc acaccgatgc ccaattcatg atagaagtca
    25321 tacatttctt cggcgccgaa acgcgcagac agtttgcttg tgccgacgtt ggacgatttc
    25381 tgcataatgc cgcgcacatc caaagagggg taaacatggg tatcgtcgcg cacgggagac
    25441 ggtccgattt tataaggctg cgtattcagc cgttcgttca aatcggtttt gcccgcatcc
    25501 aatgccttcg caatcacgaa cggtttgatt gccgaaccag gttcgatcat atcggttacg
    25561 gcacggttgc gccgctgttc gctgtctgcc cggccgggtc tgttgggatc gtaggcgggc
    25621 gtattggcca aggcgaggat ttcccccgtg cgggcatcca aaaccaccac cgttccggct
    25681 tttgcctgat ggtattcgac cgccttgttc aactcttcat aggccaaggt ctgaatcctc
    25741 tgatcgaggg aaaggatgat gtctttgccg ttttgcggtg ctttattgcg cggggagtcc
    25801 aagctgtcca caatattgcc ctgccggtcc cgcaaaacaa cttccgcgcc gtcttcgcca
    25861 tacaggctgt cttcaagcga aagttccaaa ccttcctgac ctttgccgtc aatatcggta
    25921 aatccgatga cgtgtgcaaa caggttgccc atcgggtaat ggcgttttaa ttctttttca
    25981 aatacaaagt tttccaaacc caaggctttg acctcttcgg caaccttggg atcgagctgc
    26041 cgcttgatcc aaataaacga cttgcctttc tgttcgagtt tgttcctcaa aacatcgacc
    26101 ggcacatcga caagctcgga caggcgttcc aattgggcgg cagacggcat ttccttcata
    26161 tctttaggca cggcaaacag ggactccgtc ggcgcgctca acgccaaaac cgcaccgttc
    26221 cggtccgaaa ccgtaccgcg tgtagccggc aatgcttgag tccgcacaat ccggttgtcg
    26281 ccctgttctt tcaaaaagtt atacgttacc gtctgcagat acagcccgcg ggcaatcaga
    26341 caggcaaaca agaccgccat tgccatcagg acgaagctaa tccgtccgtt actggtcatc
    26401 ggctttttga cctgctcttc tttgggcagc atccggggct tatattcgct tttaatcaac
    26461 atttttactt ctcgttatta ttatcctgat gcaggaatcc gattccggca cacaggctgc
    26521 ttctatcttt gatgttccac cataaaggta ttgcccgaaa ccggcggatg gaggttttgt
    26581 ttttctgccg ccgccctgat cgcttcgtga ttcgccaaac gcgcctgttg caccctcatt
    26641 tgcgcataat cctgctccaa ggcgatttcc tgttttttcg ccttgtccaa agctgtgaag
    26701 ttgagcctgt actggttttg ctgcatcaca acggaaaaag cggaaacgca caccgcaagc
    26761 agcagaaaga aattcgattt gttcattgcc gttcagacgt ttttctctgt tattattccg
    26821 gtatcggacc ggcagtccgc tccgccacac gcaaaactgc gctcctcgcc ctcgggttgg
    26881 cggcaatttc cgcttcaccc ggctttaatg ccctgcccac gattttcagg ggcggctcgg
    26941 gcaaatccgc ttctctgacc gccgcccagc tcggcagggg ctcgtgttgc gaatattttt
    27001 tgacaaactg cttcacaatg cggtcttcca acgaatggaa agcaatgacc gccaaacgcc
    27061 cgccctcttt cagacggcac atgacctgcg gcaataccgc ccctacttct tcaagctcgc
    27121 ggttaataaa gatgcggatt gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc
    27181 gagtacggac gttttgtgcc acgatctgcg ccagcttgcg ggttgtatcg attggacttt
    27241 ccgcccgttg cgcgacaatg gcgcgcacaa tctggcggct aaaccgctct tcaccataat
    27301 tcttgattac ctcgtgcaaa tcctgttccg acgcaaccgc tatccactct gcggcagaca
    27361 taccgcgcgt cgtatccata cgcatatcca aaggggcatc gaaacggaag ctgaaaccgc
    27421 ggctgccgtc atcgatttgc ggagacgaaa tccccaaatc aaacagcgca ccgtccacct
    27481 tgccgatacc caaaccgtcc aatgccgtct gaaacgaagc aaaaccgcca tgcacgacac
    27541 cgacccgttt gtccgaacgc gccagctctt ctgccacagc aatcgcctgc gggtctttgt
    27601 cgaaaacaat caaccgcccc gcatcgccca aacgcgacaa aatcagccgg gaatgccctc
    27661 ccctgccgaa cgtaccgtcc acatagacac cgtcttcgcg cacggcaagc gcatccacca
    27721 cctcattcag caagaccgtg atatgccggt aactttctgc tccactcaca attgcaaatc
    27781 cgtctgactc aactggaagg caagttcgtc aggatcgtca tccaaagcct gaaccatctc
    27841 agcctcccac tgctcgcgac cccaaagctc caaacggttg gcacggccga ccaaaacaac
    27901 ttcacggtcg aaatccaccc ttttcctcag tccggcagga accagcaccc tgccggcgct
    27961 gtcccattcc aaaatttccg cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg
    28021 gttatccgcc acttttaagt ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg
    28081 gtacatcaac agcttatgtt tcgactcgag cgttgccact acggcaggcg tatagaggcg
    28141 cgacagaatg tcgcggaatt tggcaggaac agccaaccgc cccttactgt cgatgcttaa
    28201 ttcgtgtgcg ccgccgaaca tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa
    28261 gagacacttt gccccacaat tccccaccaa tcgacactat aagaaatttt aaacactcgg
    28321 tcaaatcagg gcatgaaaac tcattaacat atctgaaatt tttattctgt ttaaaaacaa
    28381 taagataaaa aatgacgaca acggcacggc gggtgcagta cagagtgacc gaaccaataa
    28441 acaactatat atgattaatt taataatata aacacaatat atagtattaa gataaagcca
    28501 tgacagcacc cgtaccaacg tgtaatatgt cgggaaatcc aataaattta cacaagctaa
    28561 catttatcat gctcctcccc tcccccgaag cacggcaatc ctcgctcaat ctgcaaaccc
    28621 tcattgccga agaaatcggc aaacacggca attggattcc attttcacgt tttatggaat
    28681 tggttttata cgctccgcaa tacggctact acaccggcgg cagccataaa atcggcaata
    28741 ccggggattt tattaccgca ccgaccctca cccctctgtt tgcacagaca ctggcacgcc
    28801 aacttcaaga actcctgccc caaacggcgg gcaatatcta tgaattcggc gcgggaaccg
    28861 gacagcttgc cgccgatttg ttgggcagcg tttcagacag catcaactgt tactatatta
    28921 ttgaaatatc gccggagctg gcagcacgtc agaaaaacct gattcaagcc cgtgcaccgg
    28981 aagcatctca aaaagttgtc cacttgaccg cacttcccga agcgtttgac ggcatcatca
    29041 tcggcaacga attactcgat gccatacctg tcgaaatcgt ccgtaaaaat gaaggaggat
    29101 tactcgagca catcggcgtt tgcacggata acggccgttt cgcctattct gcaagaccct
    29161 tgcacgaccc gtcgctttcc acctcggctt ccctctattt tcctcaaaca gattatccct
    29221 ataccagcga actgcacccg caacaatatg cctttatccg cacccttgcc tcaaggcttg
    29281 aacgcggggg tatgatattc atcgactacg gttttgatgc ggcgcagtat taccacccgc
    29341 aacgcaatca aggcacattg atcggacact accgacacca cgttatccac aatcctttcg
    29401 acttcatcgg gttggcagat ctgaccgccc acgtcaactt taccgacatt gcccaagccg
    29461 gtacggatgc aggactggat ttgacaggct atctgcccca gtcccatttt ctgctgaatc
    29521 tgggcataac cgaactgttg gcacagacgg gtaaaacaga ttcggcagcc tatatccgtg
    29581 aagccgccgc tgttcaaaaa ctgattgacc agcatgaaat gggcgaactg tttaaagtca
    29641 tcgcattcgg aaaaaatatc ggcatcgact gggcaggatt ccgcttcggc gacatctgcc
    29701 acaaactcca accctcgtgc cgtctgaatc cgcttcagac ggcataaact ttttaacatt
    29761 taaaaacaag aaactaattc aaaattaaaa aatacggctt gtcaaaaaaa caaaaaaaca
    29821 tataatagct aattcacgaa acggcgaatt agctcagtcg gttagagcag aggaatcata
    29881 atccttgtgt ccggggttcg agtccctgat tcgctaccag attttcgggg gtatagctca
    29941 gttggtagag cgcttgcatg gcatgcaaga ggtcagcggt tcgatcccgc ttacctccac
    30001 cagataaaaa agcacagacc gtaaaaggta tgtgcttttt tattgcctga ttgccggaaa
    30061 ataaagaata aaccactgcc ttcaaaacag gcaatcgact ttaaacctat tgccccgcct
    30121 gtcctgattt tatcagcccg ccagacaaac ccgacctgaa taatgtcttc aggtcgggtt
    30181 tatggtttca ttcccaactt atccagcccg acaaccacaa tattatgatg acaatgccga
    30241 aaacaatgcg gtaataggca aacgggatat agtttttctt ggaaacaaac ttcagcagtg
    30301 ctttaaccgc taccaaaccg gaaacaaaag cggcaataaa gcctatcaaa atcaaaccga
    30361 catcatgcag ggtgaaaaat cggtaatgtt tcaggacatc ataagccgtt gctgcaacca
    30421 tcatcggaac ggccaagaaa aatgaaaact ccgttgccgt tttccgctcg attccccaaa
    30481 gcatcccgcc cataaccgta ctgcccgaac gggacgtacc cggaaccagt gcaaacactt
    30541 gggcaacacc gatcatcaac gcatcaatcg gacgcaatgc atcaacatcg gcaattttag
    30601 gctctgctcg gctttggcgt ttctccaccc acaaaataaa aaaaccgccc aaaaccagca
    30661 tgactgcaac actcaagggg ttaaacagat actctttgat ttgtttgtcg aacaacagcc
    30721 ccatcacggc ggcaggtata aaagcaatgg caagattgag gacgaaacgg ttggctttcc
    30781 ggtcttttcc cacgccatgc aacacattgc tgaaacgctg ccggtattca aacactaccg
    30841 ccaaaaccgc accgagctgg atggcaattt caaaaacctt gtgattgctg tgaaaaccaa
    30901 tcagattgcc gaacacaatc aaatgtccgg tgctggaaat cggtaaaaat tcggtaaaac
    30961 cttctaccaa gcccatcatc agggctttca ggacaatcag aaaatccatt gcttgcgctt
    31021 ctttcggata cgggagttcg gctatttctg tacagcaggg gtctgacgct tgcgttcttc
    31081 cctgactttg gcaaccaatt ctttaatcgt atgaacgccg ccatcaaagc cattattgaa
    31141 gataacgcgg tattttccgc cgacaataac ggtcggcgtg ctgtcaatac cgtattgttc
    31201 cgtcagtttc tgcattttta atgcgacggc ggcagcttcg ggggaatcat aggcgcgcat
    31261 cagttttttg ccgtcaaaac ctttttgaga taaagcccat tttccggcaa cagccctgtt
    31321 ttccaaacgg attttttgtt cgtaaactgc tttaaacaca gcagagtttg cctgatattt
    31381 caaacccgac agcttgaccg cagcagccat tcttgccaga ccgagcattt caggccgcca
    31441 gaccacgtgc tccgtccgca gataagtatc agacggcaat gccttgccca gtttcaataa
    31501 caaaggatcg aaatgatggc aatgtacgca aaaatagccg aaaaattcca aaacctcaat
    31561 ttttcccggc tgttcttgag gaatgggttt atccaacaca agatagtctt ccccttccgt
    31621 cagggcatat gcctgcgcgg acaacactgc cgacagcagc agcggcaaca gatgtttaaa
    31681 cttcataatt atttgccttc aatcgcacgg atcaggctgg caaccccatg ctttttcaac
    31741 tcgtcctgca tttttttcac cgcatcggca gacatattgc cgctttgcac gcggtaaagc
    31801 gttttatgtc ccgcctgata gccgaccact tcggaagata tgcccaagat tgccagtttg
    31861 gcacgctgcc cttccgcgct ccggcggtcg gcatacgcgc ccatttgcag ataatgcgtt
    31921 gcttccgcct tgccaaacgt tttcattttc tgcacttctt tggcagcggc actgcgcgct
    31981 ttttcgatgc tgccgctgtt gaggatttgt tccggggtcg gtttgggtgc aactttttcc
    32041 ttcgccgcct ttttctcttc ttttgaagct tttttctctg tttctttaga cggttttacc
    32101 gcttgttttt taaccgtttc ggcatctttc ttctgcgctt tttccctgac ggtttgttca
    32161 cgctcttccg tcagtgcttt cttgcgcact gcctgtccgt ccggctcttc ccgttccggc
    32221 tcgcccgcct tttcttcaac ctcgtcggct ttgtcggcaa cgggctgctt gtcggcagct
    32281 ttttccgcat ccgactgctc tgcctctttc gcaacatccg gttcggacaa ggcgttttga
    32341 tcggccggtt caggttggat gtcttcctta ggctggtttt tcagtttcag gatttccgtt
    32401 tctgcaggct gcttcgacgg agccgggatt ttgaacgcat tttgaccgcc ctggttcaga
    32461 taaagcaaaa taccggcaat aatgaccgtt gccagtatca aaccgaagaa gaaaccggac
    32521 agaccttttc cggattggga aaatttgttc ataaacatac cttaatgtgt ttcagacggc
    32581 attaacgccg tttgcttacg ggcggatatt ctaacaatat cgccatattt gggcaaaacc
    32641 tgcttccatt cccgttccta taaagcacga cggaagccta tggctttcat gacgtggctt
    32701 ctgccgactt cttcatcgcc cgccaaatcc gccaatgtac gcgccacgcg cataatgcgg
    32761 tggaagctgc gggcggaaag agacaatttt tccagcaggc tacccaaggc ttcctgcgct
    32821 tctttttgga tgcaggcttg ggaatcgagt tcgctgacgc tcaaagcggc gttgactttg
    32881 ccctgccgtg cgtattgttt gccgcgggcg gcgatgacgc gttccaaaac ggacgcactg
    32941 ctttcgcccg cttcctgctg catcagttcg gcggcggaca ggctggggac ttcgatggtc
    33001 aaatcgatgc ggtcgagcag cggaccggaa attttgcttc ggtaacgcgc gacactttcg
    33061 ggcgtgcagc ggcagggttt gacgggatgc ccgagataac cgcacggaca cgggttcatg
    33121 gcagcgacaa gctggaattt ggcgggatag acggcttggc gcgccgcgcg ggaaatgtgg
    33181 atttcgccgt tttccaacgg ttcgcgcaaa acctccaaaa ctttgcggtc gaactcgggc
    33241 aattcgtcca aaaataaaac gccgtgatgc gccaatgaaa tctcgcccgg acgcggatcc
    33301 gagccgccgc cgaccatagc cgccgcgctg gcgccgtgat gcggactgcg gaaaggacgg
    33361 ttgctgtcga gttgttgttg gtggttgggc aggagcgaac gcaatgccca aacttctacc
    33421 aattcgtctt cggccagcgg cggcaggatg ccgggcagcc gttgggagag catagacttg
    33481 cctgttcccg gcgggcccat catcaagagg ctgtgtccgc ctgcggcggc gatttccaaa
    33541 gcaaggcggg cagtgtgctg acctttcaca tcacacaaat caggttgtcc gccatgttca
    33601 gacggcattt gcggtacgga acattcggtt tgcgccaaag gttcgatacc gttcaaatga
    33661 gcggcgactt cgcccaaaga gcgcgcgccg taaacggtaa tgccgcgcat cacggcggct
    33721 tgtcctgcgt tttcttcagg caaaacaaat gcacgttttg cctgcatacc ctgccacgcc
    33781 atcgccaacg cgccgcgcac ggggcgcaac aaccccgaca gtgccaattc ccccgcaaac
    33841 tcgtattccg ccagcttttc gggtgcaacc tgccccgatg cggcaaggat gccgattgct
    33901 atcggcaaat cgaaacgccc cgactctttg ggcaggtcgg caggggcgag gttgacggtg
    33961 atttttttgg cggggaattc aaaaccgctt tgaataatgg cggcacggac gcggtcgcgg
    34021 ctttccttta cttccgtatc gggcagtccg acgatgttga aatgcggcag gccgttggca
    34081 aggtgggctt ccacttcgac caacggcgca ttcataccgc tcaaggcgcg gctgtaaacc
    34141 aaggcaagcg acatatttca gacggcctta ttcgccggct tcggtttgct gcctgatttc
    34201 ggcgacggct tcttcggcag cggcttcagc cgcttccaat gctgcccgtt cgggattttg
    34261 cgcggcttcg agtttcgcca gacgtgcttc caaagccgcc agtttggtac gggttttaat
    34321 caaaacctgc tgctggatgt cgaattcttc gcgcgtaacc aaatccatac ggttgaacgc
    34381 gccgcccagc atcgccttaa tatttttttc cacatctttg gcagggctgt tggcgatggt
    34441 ttcgctgatt ttcgagccga cttcttcaaa aagctgcttg ccgaacataa tctgcatcct
    34501 tcctgaacat atcaaattca agcggctatt gtataaggaa aaatgccgtc tgaaaacggg
    34561 cggcggataa tcgtcaaaac ataccgcgcc ccctttgcgg ttgctaaaat tacacttcga
    34621 gacacctgcc gcctgaaaac ggatttcata ttccgcgcaa cgcccgatta cattacaaga
    34681 cactacaaaa caatatgctg ttttaaatga tttttccgat gcgcatcgtt tcaaactcgg
    34741 ctttttaagc cattaagtgc tttgcaaaca acagaaattg ggttatgctg aaacggatta
    34801 tttacaattt catatagttt cattacatat cttattgtaa ttgaagataa tttatccgaa
    34861 tcccctttcg ggtatccgga ttttccgttg tactttcatt agaaaaacat ttcaggcgca
    34921 agttgcttgc aatttcaaag cctgtaattc tgaagtttta gacgaggaat agcacatgaa
    34981 tcccatgtac atcacttttg caatctattt ggttgcagtc cttctcatcg ggcttgccgc
    35041 ttatttttcc acgcgcaatt tcgatgatta tattttgggc gggcgcagcc tgggcccgtt
    35101 tgttacggcg atgtcggcag gcgcgtccga tatgtccggc tggcttttga tgggtctgcc
    35161 gggcgcgatt tatttgagcg gtttgaatga ggcttggatt gccatcggcc tcttggtcgg
    35221 cgcgtatttc aactggctct tggtggcggg ccgcctgcgc gtgcataccg aatatgccaa
    35281 caatgcgctg acgctgccgg attatttctt ccaccgcttc ggcgcgggcg gacacttgat
    35341 gaaagtggtt tccgcactga ttatcctgtt tttcttcacg atttattgcg cctcgggcat
    35401 tgtggcgggc gcaaccctgt tccaaagcct gtttgaaggt atgacttaca atcaggcaat
    35461 gtggctgggc gcgggcgcga ccatcgccta taccttcttg ggcggcttct tggcggtcag
    35521 ctggacggat acgctgcagg cttctttgat gattttcgcc ctgattttaa cgccggtgat
    35581 ggtctatctg ggcttgggcg gcgcggaaca gatgtctgcc gcgattcaaa gcgtcgccgc
    35641 aggcacgggc aaagaatacg gcagcctgtt tgccggtacg accgtcatcg gcatcatttc
    35701 caccgccgcc tggggcttgg gctatttcgg acagccgcac attttggcgc gctttatggc
    35761 cgccgaaagc gcgaaatcgc tggtatccgc acgccgcatc ggcatgactt ggatggcgtt
    35821 gtgccttgcg ggcgcggtag cggtcggtta tttcggcatt gcgtatttcg gtgcaaaccc
    35881 cgacaaagtt tcttctatga gcggcaacca cgaacgcatc ttcatcgcgc tttccaccct
    35941 gctgttcaac ccttggatcg ccggcatcat tttgagcgcg attttggcgg cggtgatgtc
    36001 caccctgtcc tgccagcttt tggtttgctc cagcgcgatt accgaagact tctacaaagg
    36061 ctttttgcgt aaaaacgcgc aacagtcgga attggtatgg gtcggccgcc tgatggtatt
    36121 ggcgattgcc gtgatttcca tcctgattgc ttccgatccg aacagcaaag tattgggact
    36181 ggtgtcttac gcgtgggcag gtttcggtgc ggcattcggc ccgattgtga ttctgtccgt
    36241 attgtggaaa cgcatcactg cctacggcgc gttgtcaggc atggttgcag gtgcgtcaac
    36301 cgttgttgtg tgggcggaat gggtaaaaaa accggctcga gcggcaggcg aaagcggctt
    36361 gttgacgatg tacgaaatcg tcccgggctt tattgtttgc ttgatcgttg ccgtgttggt
    36421 ttccctgttc aacaaagaac cttcgcgcga aatccaagaa cgctttgaaa aagccgacgc
    36481 agattaccgc gccgcccgat aaaccccttt cagacggcac ggcatcccgg ttcgtgccgt
    36541 ctgaacccgc ttccgggcca aacacaaata aaccttgcct gcatacaggc cttatttgtg
    36601 ttttacccgt ttcattttca aagaaacgga agcaccgatt ctgcccgcat ttgaagcctt
    36661 gtatttcatc tgcggattta ttacgatatt accgtattca ggccgcaccg atgccgtctg
    36721 aaccccccga aaaaactttg gaggatccaa aaaatgtttc attttgcatt tccggcacaa
    36781 actgccctgc gccaagcgat aaccgatgcc taccgccgta atgaaatcga agccgtacag
    36841 gatatgttgc aacgtgcaca gatgagtgac gaagagcgca acgccgcctc cgagcttgcc
    36901 cgccgtttgg ttacccaagt ccgcgccggc cgcaccaaag ccggcggcgt ggatgcgctg
    36961 atgcacgagt tttcactctc cagcgaagaa ggcatcgcgc tgatgtgtct ggcagaagcc
    37021 ctgctgcgta tccccgacaa cgccacgcgc gaccgcctga ttgccgacaa gatttcagac
    37081 ggcaactgga aaagccattt gaacaacagc ccttccctct tcgtcaatgc tgccgcctgg
    37141 ggtctgctga ttaccggcaa gctgaccgcc acaaacgaca aacaaatgag ttccgcactc
    37201 ggccgcctga tcggcaaagg cggcgcaccg ctcatccgcc aaggcgtaaa ttacgccatg
    37261 cggcttctgg gcaaacagtt cgtcaccgga cagaccattg aagaagccct gcaaaacggc
    37321 aaagaacgcg aaaaaatggg ctaccgcttc tccttcgata tgttgggcga agccgcctac
    37381 acccaagccg atgccgaccg ctactaccgc gactatgtcg aagccatcca cgccatcggc
    37441 aaagatgcgg caggacaagg cgtttacgaa ggcaacggca tttccgtcaa actctccgcc
    37501 atccatccgc gctactcgcg cgcccaacac ggccgcgtga tgggcgaact gttgcctcgc
    37561 ctgaaagaat tgttcctttt gggtaaaaaa tacgatatcg gtatcaacat cgatgccgaa
    37621 gaagccaacc gtctggagct gtctttggat ttgatggagg cactcgtttc cgaccccgat
    37681 ttggccggct acaaaggcat cggtttcgtt gttcaagcct accaaaaacg ctgcccgttc
    37741 gtcatcgact atctgattga cctggcccgc cgcaacaacc aaaaactgat gatccgcctc
    37801 gtcaaaggcg cgtattggga cagcgaaatc aaatgggcgc aagtggacgg cttgaacggc
    37861 tatccgacct acacccgcaa agtgcatacc gacatctcct acctcgcctg cgcgcgcaaa
    37921 ctgctttccg cgcaagacgc ggtattcccg caattcgcca cccacaacgc ctacaccttg
    37981 ggcgcaatct accaaatggg caaaggcaaa gattttgaac accaatgcct gcacggtatg
    38041 ggcgaaactt tgtacgacca agtcgtcggt ccgcaaaact taggccgccg cgtgcgcgtg
    38101 tacgccccgg tcggcacaca cgaaaccctg ctcgcctact tggtgcgccg cctgttggaa
    38161 aacggcgcga actcgtcttt cgtcaaccaa atcgttgatg aaaacatcag catcgaccgc
    38221 ctgattaaga gcccgttcga caccatcgcc gaacaaggca tccacctgca caacgccctg
    38281 ccgctgccgc gcgatttgta cggcaaatgc cgtctgaact cgcaaggcgt ggacttgagc
    38341 aacgaaaacg tattgcagca gcttcaagaa cagatgaaca aagccgccgc acaagacttc
    38401 cacgccgcat ccatcgtcaa cggcaaagcc cgcgatgtcg gcgaagcgca accgattaaa
    38461 aaccctgccg accacggcga tgttgtcggt acggtcagct ttgccgatgc cgcgcttgcc
    38521 caagaagcgg ttggtgcagc cgttgccgcg ttccccgaat ggagtgcgac acctgccgcc
    38581 gaacgcgccg cctgcctgcg ccgtttcgcc gacctgctcg aacagcacac ccccgcgctg
    38641 atgatgcttg ccgtgcgcga agcgggcaaa acgctgaaca acgccattgc cgaagtgcgc
    38701 gaagccgtcg atttctgccg ctactacgca aacgaagccg aacacaccct gcctcaagac
    38761 gcaaaagccg tcggcgcgat tgtcgccatc agcccgtgga acttcccgct cgccatcttt
    38821 accggcgaag tcgtgtccgc cctggcggca ggcaacaccg tcatcgccaa acctgccgaa
    38881 caaaccagcc tgattgccgg ttatgccgtt tccctcatgc acgaagccgg catcccgact
    38941 tccgccctgc aactcatcct cggcgcaggc gacacgggtg cggcattgac caacgatgcc
    39001 agcatcggcg gcgtgatttt cacaggctcg accgaagtgg cgcgcctgat caacaaagcc
    39061 cttgccaaac gcggcgacaa tcccgtcctg attgccgaaa ccggcggaca aaacgccatg
    39121 attgtcgatt ccaccgcact tgccgaacaa gtctgcgccg acgtattgaa ctccgccttc
    39181 gacagcgcgg gacaacgctg ctccgccctg cgtatcttat gcgtccaaga agacgttgcc
    39241 gaccgtatgc tcgacatgat caaaggcgcg atggacgaac tcgtcgtcgg caaaccgatt
    39301 cagctcacta ccgatgtcgg tcccgtcatc gatgccgaag cgcagcaaaa cctgttgaac
    39361 cacatcaaca aaatgaaagg cgttgccaag tcttaccacg aagtcaaagc cgccgccgat
    39421 gtcgattccg aaaaatccac gttcgttcgc cccatcctgt ttgaattgaa caacctcaac
    39481 gaactgcaac gcgaagtctt cggccccgtc ctgcacgtcg tccgctaccg cgccgacgaa
    39541 ctcgacagcg tcatcgacca aatcaacagc aaaggctacg ccctgaccca cggcgtacac
    39601 agccgcatcg aaggcacggt acgccacatc cgcagccgca tcgaagccgg caacgtttac
    39661 gtcaaccgca acatcgtcgg tgcagtcgtc ggcgtacagc ccttcggcgg acacggtctg
    39721 tccggcacag gacccaaagc aggaggttcg ttctacctgc aaaaactgac ccgcatcccc
    39781 gaatgggttg ccccgaccct gagccaaatc ggacaggcag acgaagccgc gctcaaacgc
    39841 ctcgaagccc tgattcacaa actgccgttc aacgccgaag agaaaaaagc cgcagcggcc
    39901 gctttgggac acgcccgcat ccgcaccctg cgccgggcag aatccgtcct gaccggcccg
    39961 accggcgagc gcaacagcat ctcatggcac gcgccaaaac gcgtctgggt acacggcggc
    40021 agcctcctgc aggccttctg cgccctgacc gaacttgccg ccgccggcat ccgaaccgtc
    40081 gtcgaacccg gcagcccgtt ggcttcctac tcggccgact tggacggttt gctgcaaatc
    40141 agcggcaaac ccgaaaacac cggtgtgaac cacgtcgccg ccctaagccc actggacagc
    40201 agccgcaagc aggaactggc aaaccgcgac ggcgcactca tccgcatcct cccgtccgga
    40261 caaggcttgg acatcctgca agtgtttgaa gaaatttctt gcagcgtcaa caccacagcc
    40321 gccggcggca acgccggcct gatggcggtt gccgactgat tttgccaaac cgcccgggcg
    40381 cggcccgtga acaaatgccg tctgaaaacc tttcaggcgg cattttatag tggattaaca
    40441 aaaatcagga caaggcggcg ggccgcagac agtacaaata gtacggcaag gcgggccaac
    40501 gctgtactgg tttaaattca attcactata tgtgttggca catcgctcca aacctgatat
    40561 aatccgcctt tcaacatcag tgaaaatcgt tctttttagt cagttaacat taaatttcgg
    40621 agtcgaaaaa tgaatccagc cccaaaaaac ttctcttctc ttctcttctc ttctcttctc
    40681 ttccgcagcg caggcggcaa gtgaaggcaa tggccgcggc ccgtatgtgc aggcggattt
    40741 agcctacgcc gccgaacgca ttacccacga ttatccgaaa ccaaccggta caggcaaa
//
LOCUS       DAXDZG010000007        40682 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXDZG010000007 DAXDZG010000000
VERSION     DAXDZG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40682
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.007"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            164..544
                     /locus_tag="ACFB78_000316"
     CDS             164..544
                     /locus_tag="ACFB78_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC1268396.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(745..1587)
                     /locus_tag="ACFB78_000317"
     CDS             complement(745..1587)
                     /locus_tag="ACFB78_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC1268397.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPIDGNSLADIAAMVYYGTRMIESGKDV
                     SAPLKQLGNRQAVTMLTVWHETRSILKRSAAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(2086..2262)
                     /locus_tag="ACFB78_000318"
     CDS             complement(2086..2262)
                     /locus_tag="ACFB78_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268398.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(2590..3081)
                     /gene="dtd"
                     /locus_tag="ACFB78_000319"
     CDS             complement(2590..3081)
                     /gene="dtd"
                     /locus_tag="ACFB78_000319"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC1268399.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(3155..3913)
                     /locus_tag="ACFB78_000320"
     CDS             complement(3155..3913)
                     /locus_tag="ACFB78_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1268400.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(3983..4849)
                     /locus_tag="ACFB78_000321"
     CDS             complement(3983..4849)
                     /locus_tag="ACFB78_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1268401.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(4898..5176)
                     /locus_tag="ACFB78_000322"
     CDS             complement(4898..5176)
                     /locus_tag="ACFB78_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC1268402.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(5176..5466)
                     /locus_tag="ACFB78_000323"
     CDS             complement(5176..5466)
                     /locus_tag="ACFB78_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC1268403.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(5469..5999)
                     /locus_tag="ACFB78_000324"
     CDS             complement(5469..5999)
                     /locus_tag="ACFB78_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC1268404.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            6307..8623
                     /gene="tspA"
                     /locus_tag="ACFB78_000325"
                     /pseudo
     CDS             6307..8623
                     /gene="tspA"
                     /locus_tag="ACFB78_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(8796..9212)
                     /gene="gloA"
                     /locus_tag="ACFB78_000326"
     CDS             complement(8796..9212)
                     /gene="gloA"
                     /locus_tag="ACFB78_000326"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC1268405.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(9237..10406)
                     /gene="lapB"
                     /locus_tag="ACFB78_000327"
     CDS             complement(9237..10406)
                     /gene="lapB"
                     /locus_tag="ACFB78_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC1268406.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHVYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(10417..10731)
                     /locus_tag="ACFB78_000328"
     CDS             complement(10417..10731)
                     /locus_tag="ACFB78_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC1268407.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSANLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            11041..12039
                     /gene="ilvE"
                     /locus_tag="ACFB78_000329"
     CDS             11041..12039
                     /gene="ilvE"
                     /locus_tag="ACFB78_000329"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC1268408.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(12284..13069)
                     /gene="fabI"
                     /locus_tag="ACFB78_000330"
     CDS             complement(12284..13069)
                     /gene="fabI"
                     /locus_tag="ACFB78_000330"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC1268409.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(13224..14045)
                     /gene="dapD"
                     /locus_tag="ACFB78_000331"
     CDS             complement(13224..14045)
                     /gene="dapD"
                     /locus_tag="ACFB78_000331"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC1268410.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(14255..15898)
                     /gene="pgi"
                     /locus_tag="ACFB78_000332"
     CDS             complement(14255..15898)
                     /gene="pgi"
                     /locus_tag="ACFB78_000332"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC1268411.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            16408..17640
                     /gene="pilG"
                     /locus_tag="ACFB78_000333"
     CDS             16408..17640
                     /gene="pilG"
                     /locus_tag="ACFB78_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC1268412.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            17714..18574
                     /locus_tag="ACFB78_000334"
     CDS             17714..18574
                     /locus_tag="ACFB78_000334"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC1268413.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            18571..19203
                     /gene="coaE"
                     /locus_tag="ACFB78_000335"
     CDS             18571..19203
                     /gene="coaE"
                     /locus_tag="ACFB78_000335"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC1268414.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            19196..19405
                     /locus_tag="ACFB78_000336"
     CDS             19196..19405
                     /locus_tag="ACFB78_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC1268415.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(19491..21167)
                     /gene="pilB"
                     /locus_tag="ACFB78_000337"
     CDS             complement(19491..21167)
                     /gene="pilB"
                     /locus_tag="ACFB78_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC1268416.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(21413..21484)
                     /locus_tag="ACFB78_000338"
     tRNA            complement(21413..21484)
                     /locus_tag="ACFB78_000338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21449..21451),aa:Arg,seq:cct)
     gene            complement(21505..21951)
                     /locus_tag="ACFB78_000339"
     CDS             complement(21505..21951)
                     /locus_tag="ACFB78_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC1268417.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(21955..22929)
                     /locus_tag="ACFB78_000340"
     CDS             complement(21955..22929)
                     /locus_tag="ACFB78_000340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC1268418.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            23153..23461
                     /gene="rplU"
                     /locus_tag="ACFB78_000341"
     CDS             23153..23461
                     /gene="rplU"
                     /locus_tag="ACFB78_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC1268419.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            23486..23758
                     /gene="rpmA"
                     /locus_tag="ACFB78_000342"
     CDS             23486..23758
                     /gene="rpmA"
                     /locus_tag="ACFB78_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC1268420.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            23993..25177
                     /gene="ubiM"
                     /locus_tag="ACFB78_000343"
     CDS             23993..25177
                     /gene="ubiM"
                     /locus_tag="ACFB78_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC1268421.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(25366..25521)
                     /gene="rpmG"
                     /locus_tag="ACFB78_000344"
     CDS             complement(25366..25521)
                     /gene="rpmG"
                     /locus_tag="ACFB78_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC1268422.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(25553..25786)
                     /gene="rpmB"
                     /locus_tag="ACFB78_000345"
     CDS             complement(25553..25786)
                     /gene="rpmB"
                     /locus_tag="ACFB78_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC1268423.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(26036..27559)
                     /gene="farB"
                     /locus_tag="ACFB78_000346"
     CDS             complement(26036..27559)
                     /gene="farB"
                     /locus_tag="ACFB78_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC1268424.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGITALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(27583..28737)
                     /gene="farA"
                     /locus_tag="ACFB78_000347"
     CDS             complement(27583..28737)
                     /gene="farA"
                     /locus_tag="ACFB78_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC1268425.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      28978..29022
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29026..29499
                     /gene="queF"
                     /locus_tag="ACFB78_000348"
     CDS             29026..29499
                     /gene="queF"
                     /locus_tag="ACFB78_000348"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC1268426.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            29574..30260
                     /locus_tag="ACFB78_000349"
     CDS             29574..30260
                     /locus_tag="ACFB78_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC1268427.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(30610..31902)
                     /locus_tag="ACFB78_000350"
     CDS             complement(30610..31902)
                     /locus_tag="ACFB78_000350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC1268428.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGGTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            31979..32947
                     /locus_tag="ACFB78_000351"
     CDS             31979..32947
                     /locus_tag="ACFB78_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268429.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWGERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(33148..34617)
                     /locus_tag="ACFB78_000352"
     CDS             complement(33148..34617)
                     /locus_tag="ACFB78_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1268430.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSITREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRAYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(34859..35116)
                     /locus_tag="ACFB78_000353"
     CDS             complement(34859..35116)
                     /locus_tag="ACFB78_000353"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1268431.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            35042..35203
                     /locus_tag="ACFB78_000354"
     CDS             35042..35203
                     /locus_tag="ACFB78_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268432.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            complement(35300..35998)
                     /locus_tag="ACFB78_000355"
     CDS             complement(35300..35998)
                     /locus_tag="ACFB78_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268433.1"
                     /translation="MTLKASKQASKQASKQASGRVNLLTLSVLSLFCTPYAWGSDAYD
                     PVKEAEIKNKFILEAAEDRNSHVWRGPCSVSFDCFGAFSYQPGSDTRPTNVGDTADFS
                     FSDKPANGVFHYFSSGKTDQKSSEALYDEINITGKNYNSGILAVDNMPVVKKYITDNY
                     RTDLKQAVKKQLQDLYKTRPEAWEENKKRVREEIIKQFGISEDVFNKLQQKMPQKINS
                     LIEEQVLTPYILRP"
     gene            complement(36338..36583)
                     /locus_tag="ACFB78_000356"
     CDS             complement(36338..36583)
                     /locus_tag="ACFB78_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268434.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            36695..37123
                     /locus_tag="ACFB78_000357"
     CDS             36695..37123
                     /locus_tag="ACFB78_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC1268435.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            37135..37611
                     /locus_tag="ACFB78_000358"
     CDS             37135..37611
                     /locus_tag="ACFB78_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC1268436.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(37641..38129)
                     /locus_tag="ACFB78_000359"
     CDS             complement(37641..38129)
                     /locus_tag="ACFB78_000359"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC1268437.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEVALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWREAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(38197..39252)
                     /gene="aroG"
                     /locus_tag="ACFB78_000360"
     CDS             complement(38197..39252)
                     /gene="aroG"
                     /locus_tag="ACFB78_000360"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC1268438.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(39414..39641)
                     /locus_tag="ACFB78_000361"
     CDS             complement(39414..39641)
                     /locus_tag="ACFB78_000361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268439.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            39869..40165
                     /locus_tag="ACFB78_000362"
     CDS             39869..40165
                     /locus_tag="ACFB78_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268440.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            40162..40401
                     /locus_tag="ACFB78_000363"
     CDS             40162..40401
                     /locus_tag="ACFB78_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268441.1"
                     /translation="MSGVAVYLRNKNRIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVSNTTKTKTDSEKFNE"
BASE COUNT         9733 a        11444 c        10046 g         9459 t
ORIGIN      
        1 atccggacaa caaccgatgc cgcaccctgc gggcaggctt cgcactctga aaggttcagg
       61 cggcattttt attggaaggc ttttttttgc aaccgcctta cacaaagcgg ttttttgtgt
      121 aagaactgcc ataacagccg cctgtcatcg tcaggagcgg ctaatgcctt taaaattcca
      181 accaagggaa cgttcagtta tcatgtgcga ctttcgcggt tatgaagaac cggaaatggt
      241 caagaaacgc cctgtcgtcg tcatagcgcg aaacaggcac aacggcaaac tggtaacggt
      301 cgtaccctta agcagcacag agcctgtccc tttggcggac tgccaccaca aaatgagtga
      361 aaacccctta ccggacaagc cgcacatcca atgtcgggca aaatgcgata tgacggcaac
      421 agtcggattg gcacgattag accgatacaa acccaaaggg cgcgaccgtt gcatcccaat
      481 aatcagtgaa gaggattttc aggcggttaa aacagccgtt gccaaggcat tcaaactgta
      541 ctagaataaa accgttccct taaaggggct tgcaagactg ttccgaaata tgggcagccg
      601 cgcacgggcg acaggcgatg acaagccgtc cgtgcgtgtg acggggcgcg gaatgcgccc
      661 cttgtcgtat ctgcaaacgc ctacaaatcc ccaatcatcc cttcaataaa aatgccgtcc
      721 gaaggttcag acggcatttt ccgtttacct tgccgcagcc ttgccgtaaa tgccttcaag
      781 acatgcggct atgcggccgg aggcgtcttt gtccgcatat ccccgcaaca cttcgagggc
      841 cgcggcggat cttttcagga ttgaacgggt ttcgtgccag accgtcagca tcgtaaccgc
      901 ctgtctgttg ccaagctgtt tgagcggtgc ggaaacgtct ttgcccgatt caatcatccg
      961 tgtgccgtaa taaaccatag cggcaatgtc ggctaaagag ttgccgtcga tgggcagggc
     1021 gggttgcggc gcgggcaacg gttcgcggtc ggggacttcg ccggccaaac ccgtgtgcag
     1081 tgtcaatgcg tggacgtagg cgacggcttc gggcagtttg tcggcgggga tgccttcgac
     1141 ggcttcgacg ttgaagcgtt ggtgtatcat actgtacgcg gaggagtagc ctatgcgttt
     1201 gcgcccgaca agcgcggcaa ccgcccggcg caatccggta cggtcgtcgg cggtggtttt
     1261 gggagtaatt tggtagccgc ctgttttgcg gatttgaggg ataacctctt caaatatcca
     1321 gtcttggaat ttgacggctt cggctttgcg tgaacggaag atgactcggt agagattcgg
     1381 ctcgttgatg aaaagtaatt cttggttgcc tttttttgtt gcaacgctgg atttttgaat
     1441 gccctggtct tttaatggca actgacgggc attttgaatt tgaagaattt ccgcaacatc
     1501 gccaaggcaa aaccaaggtt cagcccccaa gatttgagta cggacatttt gagatttgaa
     1561 agaaaataca gaaatggtgt tttgcatttt aagctccacg aaagtttgaa agcctctctt
     1621 cgaccaagaa gaggtaggcg gacgtttcag gttggtcgac tgctcgtgga aacagcacat
     1681 ccggagatgt cccgaaagtc cgcccgttgg ctttttcagc aagggtgtat gtttaacata
     1741 cacccttcag ggaactctta tcaaaacaaa agccgcaagc gcggcaataa ccacgaaaga
     1801 aacaggcgac caaacctgtg aaccgaactt tactattatt ccgctattgc gtcaatattt
     1861 tggggggaag cacggacgga gctttgttga aagtttaaaa cttggacagt attcatggta
     1921 gaatgacctt tctatttcat ggtagataga cgcggaaaga gagttgccgc tctgctttcc
     1981 acctgcccaa atcaagttac agcttggttt gggcttcttt tttacctgca atcagcaagt
     2041 gttgaaatta taatttgtat ctacgattca tgcaagccat ttctattatt ttttcagttt
     2101 ttctaattca agttcatgtt tcagcttttc ttttacccat ttggagaagt ctaaattatt
     2161 ggaaaattcc aataaattag cttctttttc agtgttaaat gaaacgtgct ttaaaacgcg
     2221 cttagattcg tagctttttt tggattgttt ggttgggttc attcttcccc cttgttgaaa
     2281 ataatcgtag atacatttta ctcgatttaa aatcataggg caacaaaaaa accgccaagt
     2341 ttgcggcttg gcggcggtcg ggctatttta agaactcttt tcggattttt tctttaaccc
     2401 attgggaaaa actaatttta tttgcacctt atttgcacct tttttataaa tataattcaa
     2461 atatttgatt tatatattaa tttaattaaa tattacatga ccctgcatag gggcgacgga
     2521 aaggcggcgc gggggaaggg tgtgtgtatt gtcgttcatt gtcgatattt catcattgcg
     2581 gactcaattt caatccggaa caaccttcat ttttggggaa atccgcgtca tgaaagagtc
     2641 cagcagtatg gttaccggcc cgtcgttgca gagcgacacc tgcatatgcg tgcggaaacg
     2701 ccctgtttcg acatgaatcc cgtgtccgcg taacagctcc gccgttcgca ggtaaagccg
     2761 ttgcgcctgt tctgccggcg cggcttggga aaacgaaggc cgccgcccgc ttgccgcgtc
     2821 ggcataaagc gtaaactgcg acaccagcag caccgcgccg ccgacatctt tcaaagacag
     2881 gttcagcttg cccgcttcgt cttcaaacac gcgcaaatgt gcgattttgt cggcgatata
     2941 gcgtgcatct ttttctgtgt cgctatgcgt tacaccgagt aacacgacaa acccgccgtc
     3001 gattttgccg caggtttccg tgccggcttc ggacacgaca tccacctttg cacctaccgt
     3061 tttctgtatg accgcacgca taagccgtcc tccttacctg aacgtgcaat gccgtctgaa
     3121 aaggcttcag acggcatcgg tatcggaaat gccattacgg tttgacaccg ttttcttcca
     3181 aaagggcatc gattttcaag cgggcttttt cctgcctcaa accttgttcc aactggtttc
     3241 tgaccaactc gaaaggctgc gcgtcggggt ttttcccgac cgcgccgagt ttgaacaggt
     3301 aatagcgttc gcccaatttg accggattgc gggtaacgtc gccacggttc ataccggcaa
     3361 actgcgaagc cagcggctcg ggaagctgct gcgccataat gaaaccgtcg aacgcctgct
     3421 cgtcgttcgg ataacgcttc atcagccctt caaaagacag ccctttgagc aggagctgct
     3481 gcgcctgacg cgcctcctct tcggttgcga agctgacctg ctgcaatttg atcatgcgga
     3541 tttgccgctc ataaaactga cgcagtgcgc tttcggaaac cgtttccgaa cgttccagaa
     3601 aacggacgta ctcctcggca taaaaagacg cttcggcgat tttaaagcgg ttttggacat
     3661 ccttatcctt atccaaacct tccttcaatg ccctgttttt caaaacttcc aaagtttgca
     3721 gccggcggac ggcatcgttt cggattgcct gcccgtccgg tctttgggac tgctccgcat
     3781 gccggtctgc ctgctgcatg atctgcgcca ccagcgtatc caccaaagcc gggtcgattt
     3841 cgggtgcttt ggctgccgca aaacctgcca acattgcagc aataactgcg gcagcggtct
     3901 ttttctgttt catggtattc cctgtcctgc gcggcggcaa aacgcctgta tgaacgtctt
     3961 gccgccgtat cccgttttca gattattttg caggtttgat gtttgcctta cccaacagcg
     4021 caccgacggc acggtcaatc cgttccgcct gaaggttgcc ggcaatctgt cctttcattt
     4081 cgtcaaaaga aggcactttc acctcgcggc tgtcgttgac ataataaacg ccgtagaaat
     4141 cgccgttttt cagcggcgtt gccgtaaatt cgcctttttt caagtcctta attgcctgat
     4201 aaagcggcgg aacaccctgt tccaaatctt tcagcggcac atatccgtcc ggcgcgccgg
     4261 tccgtttggt gcggtcgttg agcgagtatt gtttcaaaac ggcatcaaaa ccttttttcg
     4321 ccttcaaatc ggcaaccgct tttttcgcat tttcttcctt gtcggtcagg atttcgccca
     4381 actggacttc ctgcgtgcct ttataaaaac cgctgatatt gtcgtaaacg gcttttactt
     4441 cctgctcgga aaccggttgg gttttggcga tatgcagtgc gtatgcctcg ccgttcaagc
     4501 catattttac cgcctgccaa acggttttga aggacggttt cttgtcgtcg cccgactttt
     4561 tcgcttcggc acgcaatttg gcaagcgcat ctttaaactc tgccgaccgg tcgagtttca
     4621 ggcgtttcac ttcctgtgcg accacggtgt tgaccacttc gttttccagc agggattggc
     4681 gcagttgcgg cgtgtcttcg gcacggctgt tttccgcacg gaatgcggca acctgcgcat
     4741 cgatgacgga actgtcgatt ttctgaccgt taacggttgc cagcgtttgg gcaaacaggc
     4801 tgccggaaca ggcaagcagc gcaacggaag tcaggatttt tgctttcata atcttctctc
     4861 tttaaatttt aaattgaaat attcaggcag acttcattca gtccgccgta tggaaatact
     4921 cgtcaggggt agccgcccgg atgccgagcg cgtgaatcat gccgcctgaa aataaatctt
     4981 tgagcagcga tttgaccgtt ttctgacggt tcagacggct taaaccttca aaacggccgc
     5041 tgacgaccaa aacggcataa tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat
     5101 ggctctcatc gccgatttcc aacaccagag gatcgagcgt ctgcaggcgt tcgcggatca
     5161 aatcgacagc cggcattatt tgaacaccgc tttaaacggc ttgatcagca cttcgctgta
     5221 cacgccggca tgaacatagg gatcgtcttc agcccaagcc tgcgccgcat ccaaagactc
     5281 gaactgtgcc acaatcaagc tgcccgaaac acgttcggga ttgtccggca gcaggtttgg
     5341 gcctgccgtc agcagacggc cttccgattt cagcgtttcc agccgtttaa agtgttcggg
     5401 acgtgccgcc atacgcgctt catgcacatc ctccccgtct gttgccagca acataaaata
     5461 ttccacagtc aatcctcctt tttcagatag gtactcagat aaataccctg aataataaag
     5521 aaaaaaagca tcagcgcagt cgaaccgaac atcttatagt taacccattg cgcttcaaac
     5581 ctagtaaaca caaaccagtt ggcaataccc ataaaaatca gaaaaccgac ccacatatat
     5641 gtcaattttc cccatacggc atccggaagc tgaatctccc tgccgatact cgctttcaag
     5701 ccgtttttac ccgcaaggtg gctgcccagc aggaataacg ccccgcacca gaacaatact
     5761 gtcggcttcc acataatgaa gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacg
     5821 acaatcagca ccagtccgac ccactgcatc gtatccagcc ttttatgctt ccaatacagg
     5881 aaagccgcct gaaccacgcc tgcaaccaag gcaaccgccg cagcggcaat catatttttg
     5941 gtaacggtat aagtagcaaa aaacaagatg acggacaaaa ggtcgctgac aaatttcata
     6001 tccaaatgag aaagcaaatc aaataaacgc taatcataca ggcagaaccc tatctttgca
     6061 cctgcaaaac caacaaattt tgttttgatt aatgcaaata tgcacagatg tttttgaaaa
     6121 aagatggaaa tatgtcataa tttataaaat tggctttaaa atacagcttc ggccggaacc
     6181 tctgcctatc cgcaccctat cgtccgtatt gcctatgcct gatgacgaaa tttacagact
     6241 gtacgcggtc aaaccgtatt cagccgccaa cccacagggg atacatcttg caaaaacaac
     6301 agacaaatca aactgattgc cgcctccatc gcagttgccg catcctttca ggcacatgcg
     6361 cgactgggcg gactgaatat ccggtccaac cttgacgaac ccttttccgg cagcattacc
     6421 gtaaccggcg aagaagccaa agccctgcta ggcggcggca gcgttaccgt ttccgaaaaa
     6481 ggcctgaccg ccaaagtcca caagttggac ggcaaagccg tcattgccgt ttcttccgca
     6541 caggcagtcc gcgatcccgt cttggtgttc cgcatcggtg caggcgcgca ggtacgcgaa
     6601 tacaccgcca tcctcgatcc tgtcggctgc tcgcccaaaa ccaaatctgc actttcagac
     6661 ggcaagacac accgcaaaac cgctccgaaa gcagagtccc aagaaaatca aaacgccaaa
     6721 gccctccgca aaaccgataa aaaagacagc gcaaactcag ccgtcaaacc ggcgcataac
     6781 ggcaaaaccc ataccgtccg caaaggcgaa acgctcaaac agattgccgc cgccatccgt
     6841 ccgaaacacc tgacgctcga acaggttgcc gatgtgctgc tgaaggcaaa cccaaatgtt
     6901 tccgcacacg gcagactgcg tgcgggcagc gtgcttcaca ttccgaatct gaacaggatc
     6961 aaagcggcag cacccaaaaa aatcaaagcg gaacaaccca aaccgcaaac ggcgaaaccc
     7021 aaagccgaaa ctgcatccat gccgtctgaa ccgtccaaac aggcaacggt agagaaaccg
     7081 atagagaaac ctgtcgaaaa accggttgaa aaacctgaag caaaagttgc cgcgcccgaa
     7141 gcaaaagcgg aaaaaccggc cgttcgaccc gaacccaaac ccgcagtgtc cgaaactccg
     7201 gcttcggcga cagaacgcca acccggacct gtacccgctg caaatactgc cgcatcggaa
     7261 accgctgccg aatccgcccc cccaagaagc cgccgcttct gccatcgaca cgccgaccga
     7321 cgaaaccggt aacaccgttt ccgaacctgt tgcggaaaca gccagtgaag ttgccgccga
     7381 acccgttgcc gaacctgtcg aacaggtttc tgccgaagaa gaaaccgaaa gcggactgtt
     7441 tgacggtctg ttcggcggtt cgtacacctt gctgcttgcc ggcggaggca cggcattgat
     7501 cgccctgctg ctgcttttac gccttgccca atccaaacgc gcgcgccgta ccgaagaatc
     7561 cgtccctgaa gaagagcctg accttgacga cgcggcagac gacggcataa aaatcacctt
     7621 tgccgaagtc gaaactccgg caacgcccga acccgctccg aaaaacgatg taaacgacac
     7681 acttgcctta ggtggggaat ctgaagaaga gttatcggca aaacaaacgt tcgatgtcga
     7741 aaccgatacg ccttccaacc gcatcgactt ggatttcgac agcctggcag ccgcgcaaaa
     7801 cggcattttg tccggcgcac ttacgcagga tgaagaaacc caaaaacgcg cggatgccga
     7861 ttggaacgcc atcgaatcca cagacagcgt gtacgagcct gagaccttca acccgtacaa
     7921 ccctgtcgaa atcgtcatcg acacgcccga accggaatct gtcgcccaaa ctgccgaaaa
     7981 caaaccggaa accgtcgata ccgatttcta caacaacctg ttctcaaaca accatatcgg
     8041 cacagaagaa acagcttccg caaaacctgc cgcaccctcc ggactggcag gcttcctgaa
     8101 ggcttcctcg cccgaaacca tcttggaaaa aacagttgcc gaagtccaaa caccggaaga
     8161 gttgcacgat ttcctgaaag tgtacgaaac cggtgccgtc gcggaaactg cgcctgaaac
     8221 gcccgatttc aacgccgccg cagacgattt gtccgcattg cttcaacctg ccgaagcacc
     8281 ggccgttgag gaaaatgcag cggaaatcac tttggaaacg cctgattcca acacctctga
     8341 ggcagacgct ttgcccgact tcctgaaaga cggcgaggag gaaacggtag attggagcat
     8401 ctacctctcg gaagaaaata tcccaaataa tgcagatacc ggtttccctt cggaatctgt
     8461 aggttctgac gcgccttccg aagcgaaata cgaccttgcc gaaatgtatc tcgaaatcgg
     8521 cgaccgcgat gccgctgccg agacagtgca gaaattgctg gaagaagcgg aaggcgacgt
     8581 actcaaacgt gcccaagcat tggcgcagga attgggtatt tgattcccaa ctgccctttc
     8641 gcagatcaag gattccgttt cagacggcat ctttttttgc cttatcggtg taacggataa
     8701 agtttgaacc ggcacaggct caaacagcag gccgtcggca acaaaatgcc gtccgaaacc
     8761 cctaaaggct tcagacggca ttggcggcgg cggtatcagg tattcgcata ggcaaccgaa
     8821 tcgtcgccgc ttttcttttg aacgaactcg attttgcatc cgtcggggtc ttcgacgaag
     8881 gctatcacgg ttgtgccgtg tttcatcaga ccggcttcgc ggacgacgtt tccgccctgc
     8941 cgcttcacgc gttcgcaggc ttcgtaggca tcgtccactt caacagcgat gtgtccgtag
     9001 gcgttgccca agtcgtatcg ttccgtatcc cagttgtgcg tcagttccaa aaccgtgctg
     9061 tcggtttcgt cgccgtaacc gacgaaggca agggtaaatc tgccttcggg atagtctttt
     9121 cggcggagca gtttcatacc caaaacgttt tggtaggaat cgagggattt ttcgagattg
     9181 cccacgcgga gcatagtatg gagtaagcgc atttttcgtg ttcctctcgg tggtggttaa
     9241 acttcgattt tattcggcgt aaacgtctgc catttgttgc aggcgggaca gtgccagaaa
     9301 aagacttggg atttgaagtg gcagttgcgg caacggtaca tcacgctgcg ctggagctgc
     9361 cgtccgataa ccgaacgcat catgtcggca tcggctttcc aagccggatc caaatcgctg
     9421 agtttcaaac cgagcaggcg gtacacgccg ttaaggtcgg gcttgcggcg gacaagctcg
     9481 acggcggttt gcgcggcttc tttctcgccc ttaagcagca gggatttctc gtacacgaca
     9541 ttgatcaggt caagttcggg aaacgtctgc atatatcctg tcagacggtt caagccttct
     9601 tcaggttttc cctgcgcggc ataggcttcg taaagcttct cgccgaccat gctcaagtat
     9661 acatggtttt gctgctcgat ggcggcatag gcttcgacgg cggcagggaa attgccttgt
     9721 cggtgttcaa tgtcgcccaa aatcatgttg gcgcgggtgc attttttgtt ggcttcgagt
     9781 gccttgccga cattgaaacg cgcggcatcg aaattggact tgaacagcgc ggcttgggca
     9841 agttcgcaat aaaactgtgc aatctcaaac tgatatgtct gttcgtcgtg actaagaagt
     9901 tgggcggttt caaccgcttt ttcccaatcc ctgtcctgct ggtagatatt gagcaggtgc
     9961 tgtctggctt cacgcgccat ttcaccgtct tgcagcccca aaaaaatctg ttcggcacga
    10021 tcgaccaaac ccgcgctttg gtagttttgc gccaattcaa acaggacgcg cgcgcgcttt
    10081 tcgccgaccg tatcgggaga atcgagcatt gtccggtgta tgttgatggc tttgtcgttt
    10141 tcgccgcgct gacggtaaag tttgccgagg gtgaggttca aatcatacga ttgcggccgg
    10201 ccgtcgacga cttccgccaa ctcccttgcc gcgcgcccgc tgttgcggtc gaccaaagcg
    10261 tccaggcttt tataaaatcc cgaagggatg ctttttgcct gcttcaatac ggttttcata
    10321 tccacgcggg cggcaaacca gcccatcgtg aagaagacgg gcaaaaggat aatcggcagc
    10381 aggataatcc acaattcgtt gtccatatcg gctttcttaa ggctgtttgg cagattcggc
    10441 agcattttgt atcggcggtg cagtcaattt ctgtccgctc aagcgcgcac ttttcttcac
    10501 ttccgcacgc aggcggctgt tttcgccgcg caaggacagc agccgcccga acagggcaaa
    10561 cattccgaac acgatgccga cgacaaacgc gccgaacaat acgacaatca gcggcagatt
    10621 ggcactctgc cccggaagat aggaaaaggt aacggcatcc atattaatga cggcaagcag
    10681 caggaagagc agcaggataa tgattttgat gacggtatag ataagtttca tgtttgctcc
    10741 gtaaataaaa acgccgcacg gacagcttcg cactctgaac caagattgcg ttagtttaaa
    10801 cgatttgcac ggtttcggat agaatgcggc agcgtgttcg gacggcatac ggagtatgcg
    10861 attgccgaca attttaccaa atacaccgct cctttccatt tgaaaaataa cggattggac
    10921 accgcatcga cagaaaaacc cgccgcgcac ttgtcaaaac cctgtttgca ggcgtatctt
    10981 tacaaccttc aaattcaaac cgttcattga aacatatcag aataagaaag gctttacatc
    11041 atgagcagac ccgtacccgc cgtattcggc agcgtttttc acagtcaaat gcccgtcctc
    11101 gcctaccgcg aaggcaaatg gcagccgacc gaatggcaat cttcccaaga ccttaccctt
    11161 gcacccggcg cgcatgccct gcattacggc agcgaatgct ttgaaggttt gaaggccttc
    11221 cgtcaggcag acggtaaaat cgtgctgttc cgtccgaccg ccaatatcgc gcgtatgcgg
    11281 caaagtgcgg acattttgca cctgccgcgc cccgaaaccc aagcttatct cgatgctttg
    11341 gtcgaactgg tcaaacgtgc cgccgacgaa attcccgatg cgcctgccgc gctgtacctg
    11401 cgcccgacct taatcggtac cgatcccgtt atcggcaagg ccggttctcc ttccgaaacc
    11461 gccctgctgt atattttggc ttcccccgtc ggcgactatt tcaaagtcgg ctcgcccgtg
    11521 aaaatcttgg tggaaaccga acacatccgc tgcgccccgc atatgggccg cgtcaaatgc
    11581 ggcggcaact acgcttccgc catgcactgg ttgctgaagg cgaaagccga atacggcgca
    11641 aatcaagtcc tgttctgccc gaacggcgac gttcaggaaa ccggcgcatc caactttatc
    11701 ctgattaacg gcgatgaaat cattaccaaa ccgttgacag acgaattcct gcacggcgtt
    11761 acccgcgatt ccgtgctgac tgtcgccaaa gatttgggct ataccgtcag cgaacgtaat
    11821 ttcacggttg acgaactgaa agctgctgtg gaaaacggtg cggaagcgat tttgaccggt
    11881 acggccgccg tcatctcgcc cgttacttcc ttcgtcatcg gcggcaaaga aatcgaagtg
    11941 aaaaaccaag aacgcggcta tgccatccgt aaggcgatta ccgacatcca gtacggtttg
    12001 gcggaagaca aatacggctg gctggtcgaa gtgtgctgat gctttaaata aaaaaatgcc
    12061 gtctgaaacc cgtttggcgt ttcggacggc attttcgcgt tcggaccgtt tccgccgcac
    12121 ctgcagcaag tcggtacaaa ggcaatcggt taaaacaagc gtccgcattt cccatcccgc
    12181 ctgccgcaag tcgggcattt ccctggaaat acgcttcaaa cggcaaaccg ccgcccgaaa
    12241 ccgatactcg gcacggacgg cgcacggatt tgaaaacggc ggattatccc tcggtgctca
    12301 aggcattgat gctgtaaccg ccgtcaacat aagtgatttc gccggtaatg ccggacgaca
    12361 ggtcggacag caggaaggcg gcagtattcc cgacttcttc gatggtaacg ttgcggcgca
    12421 gcgggttgtg ggcggcgacg tgtcccaaga gtttgccgaa atcggcgatg ccggaggcgg
    12481 cgagcgtttt gatcgggccg gcggaaatgc cgttgcagcg gatgccctct ttacccagac
    12541 aggcggcggt aaagcggatg cctgcctcaa ggctggcttt tgccataccc atcacgttgt
    12601 aattcggaat cgcgcgtacc gcgcccaagt agctaagggc gacgatggcg gagtttctgc
    12661 cgcgcatcat cggacgggcg gcttttgcca atgcgggcag gctgtatgcg gaaatttcgt
    12721 gtgcggtgtt gaacgcttcg cggctgatgc tgtcgaggaa gtcgccgctc aaggcttctt
    12781 tcggcgcgaa gccgatggaa tgcaccaaac cgtccaagcc atcccaatgt ttgcctaagt
    12841 cggcgaacac ttggttgatt tcgtcgtcgc tggcgacatc gcagcggaat acgagttcgg
    12901 aatctaattc cgccgccatt ttgcggacgc gctcttccag tttgtccaca acgtaggtaa
    12961 acgccagttc cgcgccctgt tcgcggcagg ctttggcgat gccgtaagcg atggaacgct
    13021 cggaaatcat gccggtaatc agaatttttt tgccttgcag aaaacccatt ttgtatcctt
    13081 caaatagtgt tcggttcgtt ctaaaccgtt gattatagca aattgtccct gtttctgtgt
    13141 tttcacgttg cagcgtgcag acggcaatgc cgtccgaagc ggatttcaga cggcattgga
    13201 cgtttcaaat acggtttaag gcatcagatg ccgcgcaaga gttcgttcac gctggttttg
    13261 gcgcgggttt gcgcgtccac gcgtttgacg atgacggcac agtaaaggct gtggctgccg
    13321 tctttggaag gcaggctgcc ggacacgacg accgaacctg ccggtacgcg gccttggtag
    13381 atttcgcctg tggtgcggtc gaagattttg gtggattgac cgatgaacac gcccatcgaa
    13441 atcacgctgc cttcttcgac aatcgcgcct tcgacgattt cggaacgcgc gccgatgaag
    13501 cagttgtctt caatgatggt gggtgcggcc tgcaggggct cgagtacgcc gccgatgccg
    13561 acaccgccgc tcaagtggac gtttttgccg atttgcgcgc aagagccgac ggtcgcccaa
    13621 gtatcgacca tcgcgccttc gtcgacgtat gcgccgatgt tgacgtaaga cggcatcagc
    13681 acggcgtttt tggcaacaaa gctgccgcgt cgggcaaccg cacccggaac tgcgcggaag
    13741 cctgcgtttt tgaactcgtc ttcagaccag tcggcaaatt tggtcggcac tttgtcgaag
    13801 tatttgttca cgccgtcgtt gaggacttcg ttgtcttgga tgcggaagga cagcaacacg
    13861 gcttttttcg cccattcgtt gactttccac tcgcccacgc ccaagcgttc ggcgacgcgc
    13921 agtttgcctg aatcgagttg gcggatggtt tccaacacgg cttctttgac ttcgggggca
    13981 acggtggtcg gagtgatgtc cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa
    14041 gacataatgt ttccttggtt cgagatattt cgggacgctt gccggtattg ttcagacggc
    14101 atggcggttg gaacaaacgt gctattgtgc catacaaacg cccgcccgtc agccgcgccg
    14161 caggcttgcc ggatgccggc gtttcggaat cgggcgggcg gcaggcatat gtctttttcc
    14221 gcgcccgaat caatacagaa aggcggcggt acttttatgc cgcgccgccg cctttcagac
    14281 ggcattcgcg gtaaaacgcc atcagccctt cggtcgaggc atcgtgtacg gacgtgccgc
    14341 cttccaattc gccgatgatg gtttttgcca actgtttgcc gtactcaacc ccccactgat
    14401 cgaaggggtt gacgttccat atcgcgcctt ggacgaaggt tttgtgttcg taagccgcca
    14461 tcagcatacc cagattgcag ggcgtgaggc ggtcgagcag gatgctgttg ctggggcggt
    14521 tgccggggaa ctctttgtgc ggcgcgaggc gttcgcgttc cgcttcgggc aaatctgcca
    14581 gttcggcgcg tgcttcgtct aaggttttac ccttcatcag ggcttccgct tgggcaaagg
    14641 cgttggcaac ggtaaaacgg ctgcgtccgt cctccgcgcc ctgcgccgtc atcgggacga
    14701 taaagtcgca gggaatcagg cgcgtgcctt ggtggagcag ttggaaatag gcgtgctggc
    14761 agttgacccc ttcaccgccg aacacgatgc cgcccgtttt gcacgcggcg ggactgccgt
    14821 ctgaagcgcg gcttttgccc aaactctcca tatcgagctg gttcagccac gccggcagca
    14881 ggcgcaggtt gtggctgtac ggaacggcgg tctgcccgtc cgcgtgctgg aaattgttat
    14941 accacacggc aatcagtgcc atcagaacgg gaatgttgcg acgcgggggc gtgtggaaaa
    15001 aatggctgtc catcgcgtgc gcccccgcta gcagttcgcg gaaacgcgcc ccgccaaccg
    15061 caaccatcac gggcaaaccg acgggcgacc agacggaata gcgtccgccc acccagtcgt
    15121 acatcgcaaa cacgcgttcc gccgcaatgc cgaaactttg cgccgcttcc gtatcggcag
    15181 acaccgcgca aaaatggcgc gccgtttcgg attccgagaa ccccgcaccg cgataccacg
    15241 ccttgaccgc ctctgcattg agcagggttt ccggtgtttt aaaggacttg ctggcaacgc
    15301 aaaacattgt tgtttcgggg ttcagacggc ataagacctc atccaggcag gcagggtcgg
    15361 cattagaaac aaaatggacg gaaatctgcc gtctgaacgg ctcgagcgcc tgcacgcaca
    15421 ttgccggccc gaggtcggat ccgcctatgc cgatgtggac aaaatccgca atccgttttc
    15481 cggttatccc ctgatataaa ccgtcgtcca aactgtgtgc aaacttcaac gcacgattta
    15541 actcgcggcg gatttcgggc aacacgtccc tgccgtccgc ataaacggca tccgcaccgt
    15601 cgggcaggcg caaagccgta tgcagcgcgg cacgcccctc gctgccgttg actttcgcac
    15661 ccgtccgcaa agcacgcatt ttcccttcca aatccgccgt ctccgcaaga cggcagagca
    15721 gttgcagcgt atcttcgcca aaacggtttt tgctgtaatc gaacaacatc ccgtccaaac
    15781 gctcatgcat acgctcaaaa cggtcgggtt cggcagcaaa gcggtcgcgc aaaaggatgt
    15841 ggcacgtatc ctgataatgg cgttcgagcg cataccatgc acgggtaaag gcatccatct
    15901 gttttccttg atttttcaga accggattaa aatatagcag aatgtagtct aacaaacggc
    15961 agacacttta gcgaatctca ggaacaccgc ccgccgcgtc caataacacc ttatttccat
    16021 atggcgaaaa tatagtgaat taacaaaaat caggacaagg cggcgggccg caggcagtac
    16081 ggatagtacg gaaccggttc gcttggtgct tcatcacctt agggaatcgt cctctttgag
    16141 ccggggcggg ccaacgccgt accggttttt gttaattcac tataccctcc ggccggcatt
    16201 tgacccgcaa caatatcctg caagatttat tgtgtacgca taaatgccgg acagccgcct
    16261 aaattaaggt ataatcgcat ccgattctgt cccgtttgcc gcccgcaggc gggatgtcgg
    16321 cggttttcat cttaccggga acacgccgat actgtttttt aacgatgttt cttacatttt
    16381 attccaatta ctttacgggg ctagaatatg gctaaaaatg ggggattttc tttgttcgca
    16441 aagaaagaaa aacgctttat ctttgaaggc aggcattctg cctccgacaa actggtcaac
    16501 ggcgaagtat ccgcgtttac cgaagaagag gcacgcaaaa aactggcaaa acgcggcatc
    16561 cgcccgttgc agattacccg tgtgaaaaca agctccaagc gcaaaatcac acaagaagac
    16621 attaccgttt tcacccgcca gctttccacg atgattaaag cgggcctgcc gctgatgcag
    16681 gcatttgaaa tcgtggcgcg cggacacggc aacccgtcta tgacggaaat gctgatggaa
    16741 atccgaggcc aagtggaaca gggcagctcg ttgagccgcg cattctcaaa ccacccgaaa
    16801 tatttcgacc gcttctactg caacctggtt gcggcgggcg aaacgggcgg tgtattggaa
    16861 agcctgctgg acaaattggc aatttacaag gaaaaaaccc aggccatccg caaaaaggta
    16921 aaaaccgcgc taacctatcc ggtatccgtc atcgccgtcg ccatcggttt ggtattcgtg
    16981 atgatgattt tcgtactgcc cgcctttaaa gaagtttacg ccaatatggg cgcggagctt
    17041 cccccactga cccaaacagt gatggatatg tccgactttt tcgtctcata cggctggatg
    17101 gtgctgatcg cactgggctt tgccatatac ggcttcctta aattgaaggc gcgttcgatt
    17161 aaaatccaac ggcgtatgga tgccatactg ctgcgtatgc cgattttcgg agacattgtc
    17221 cgcaaaggga cgattgcccg ctggggcagg acgacggcaa cgctgtttgc cgccggcgtg
    17281 cctttggtcg atgtattgga ctccactgcc ggcgcggcgg gcaatttaat ctatgaagaa
    17341 gccacccggg aaatccgtac gcgcgtcatc caaggtctgt ctatgacttc ggggatgcgt
    17401 gcgacggaac tgttccccaa tatgatgttg cagatgtcct ccatcggcga ggaatcgggt
    17461 tctttggacg atatgctcaa caaagccgcc gaattttacg aagacgaggt ggacaatgcg
    17521 gtcggcaggc tgtccgctat gatggagccg attattattg tgattttggg cttggtcatc
    17581 ggtacgcttc tggtcgctat gtatctgccg ctgttcaact tgggcaacgt ggtcgcctga
    17641 tttgccgcac agatccggcg cggattggtt ctgcgccggt ttgtttttgc tttgaatata
    17701 tcaaggacaa aatatgtctg atttgtctgt attgtcgccg tttgccgtgc ctttggcagc
    17761 agttttgggg ctgctggtcg gcagcttcct gaatgtcgtc atttaccgcg tacccgttat
    17821 gatggaacgc ggctggacgg tatttgccaa agaacattta aacctgccgc tgaccgacga
    17881 tgaaagccgt accttcaacc tgatgaagcc ggattcctgc tgtcccaaat gccgtgtgcc
    17941 gatacgcgcg tggcagaaca tcccgattgt cagttacctg ctcctgcgcg gcaaatgcgc
    18001 ttcctgccaa accaaaatca gcatacgtta tcccttaatc gagctgctga ccggcgtatt
    18061 gttcgggctg gtcgcctggc aatacggctg gtcttggatt acgctgggcg gtttgatact
    18121 gaccgcgttt ctgatttccc tgacctttat cgatgcggac acccaatacc tgcccgactc
    18181 gatgacatta cccttgatct ggctggggct gatatttaat ttggacggcg gcttcgtgcc
    18241 tttgcagtct gccgttttag gtgcggttgc cggctatagt tcattatggc tcttatgtgc
    18301 agtgtataaa ctgctcacag gaaaaaccgg tatgggcaac ggagatttca aactgattgc
    18361 cgcattgggc gcgtggctcg gcatatccgc attgcccgtg ctgatttttg tttcctctct
    18421 gatcggtttg gtcgcggcaa tcgttatgcg cgtcgccaag gggcggcatt ttgccttcgg
    18481 ccccgcactg acagtttcgg gctggataat ttttacggca aacgattccg tatggcgggc
    18541 ggtcaactgg tggctgaccc atccggtgag atgacagcat gggtcggact gaccggcgga
    18601 atcggcagcg gcaaatcggc agccgcgcaa tattttgccg atttgggcgt gccgcgcatc
    18661 gatgcggacg cggcggcgca ctcgctgacg gcttcagacg gcatcgccct gccggaaatc
    18721 aggcggctgt tcggcgacac cgttttcgac acacagggtt tgttgcggcg cgacatattg
    18781 cgtaaagaaa tctttgcctc cccatcgcgc aaagccttgc tcgaatccgt gatgttgccg
    18841 ctgattttct cagaaatcaa aaaacagcaa gaaaccttta ccgatgcggt ttacggcatt
    18901 gtcgaaattc cgctgctgac ggagaagcgt caatttatca gcctgatacg gcgtgtcctg
    18961 accataagcg cacctttgga aaaacgtatc ggcagggtaa tggcacgcag cgggctgacg
    19021 cgcggcgagg tggcggacat catcagccat caggcatccg aatccgaacg cctgctgctt
    19081 gcagacgatg tactgctcaa tgacggcagc ctcaaaagcc tacgtgagaa aacaatgctc
    19141 ctgcacgcgt tttattcagg gattttcgcc tcaaaaccaa cacaaggaaa acacaatggc
    19201 tgaatcgcgg caaacacgcc ttcaagtcaa atgtccgacc tgtcaaacgg cagtagtatg
    19261 gaaaccggaa aacgcattcc gcccgttctg ttcgcaacgc tgcaaactga tcgacttggg
    19321 cggatgggca gacgggaaat atacggtttc cggtcaaacg gaaagtttgc cggaaatatc
    19381 cgaacccgac ggggcatacc gctgaccgcc ccgccttccc ggcaaacacc ctgaaaggtc
    19441 aaatgccgtc tgaaacaaac acgcttcaga cggcattttc attctcaaac ctaatcgttg
    19501 gtatttgccg ttacctcttc caatgaagta atgccctgca taattttcaa aataccggcc
    19561 cggcgcaaat ccaccatacc ctccttatag gcaacgtcca aaatacccac ttccgtaccg
    19621 ttgttcataa tcacacgctg catttcttcg ctgatgggca taacctcata cacgcccgca
    19681 cgccccttat aaccctgccc ccggcaacgg tcgcaaccga cggcgcggta aagtttccaa
    19741 tcttttgcaa gatcctcatc ggtgaaaccg acttccttca aagcagaggc agacgggcgt
    19801 tccacttcct gtttgcagct cgaacacagc ctgcgtaaaa gacgctgcgc cataatcagg
    19861 ctgaccgaac tggcaatatt aaacggcgcg acacccatat tcagcatacg cgacaacgtc
    19921 gccggcgcat tattcgtgtg cagtgtggaa aacaccatat gccctgtttg tgccgcctta
    19981 atcgcaatat cggcagtttc caaatcacga atctcaccga ccataatgat gtccgggtcc
    20041 tgacgcagga aagacttcaa agcagcggca aaagtcagac cctgcttatc attgacgtta
    20101 acctgattga tgcccggcag gttaatctcg gcagggtctt ccgccgttgc aatatttacc
    20161 gactccgtat tcaaaatatt caaacaggta tagagcgaca ccgtcttacc cgaacccgtc
    20221 gggccggtta ccagcaccat cccgtaagga cggtgaatcg cttccaacaa caattttttc
    20281 tggaacggct caaaaccgag ctggtcgatg ttcaaagacg cggcatcgga attcaaaatc
    20341 cgcatcacga ccttttcgcc aaacagcgtc ggcaatgtgc tgacacggaa atcgacaggc
    20401 ttgccgccct tttgaaaggt cagctgcatc ctaccgtcct gcggtatccg tttttcggaa
    20461 atgtccaaac gcgacattac cttaatccgg gaagcaagct gcccccttac cgcaatgggc
    20521 ggctgaacca cctcgcggag ctgcccgtcc acacggaaac ggatacgcgc attgtgttcg
    20581 taaaactcga aatggatgtc ggatgccccg ctacgcaagg catccgacaa agttttatgg
    20641 ataaacctcg gaacagggcc gtcttctgcc tcctcgttgt cgatatacag ggtgtggctt
    20701 tcctcttcct cttgcccctc cccaagctcc tgaagcagcg atgtcgaacg cgaacccacc
    20761 caatcgagca aacccgccaa ctggtcatcc tcgacaatga ccaactcaac cgcaatccct
    20821 gcggcagaaa cggttttctg aatttgcggc atctgggtcg gatcggaaac cgcaaaaaat
    20881 actttgtcgc cccgacggaa aaccggcaca cagtggaact ccaccatctg ctcctccgtc
    20941 aacaccccca tcagcaccct gtggcgcgga taatgacgca aatcaagaat cgaataactg
    21001 aacaccctcg caatcaatgc cgcaagcgac ttgggcgaaa tgacaccgtc tgaaaacagc
    21061 atcggcaaca cttccttacc cgcctgcgac tcattgtagt aatgctcggc ctgctcaaca
    21121 gtaaccacct ggttttgaac cagaatcctc agcaaaccta cgctcatacg accttatccc
    21181 caaatttatt cattgttata cctgtacaga ttttatcata aaacaacaga tttccaagcc
    21241 tgaacatctt ttacgaagcc tgaaaatcat ttcacgattc tgccgtccta aaggtcggtt
    21301 tttcaagcag gaagaaaaag ttttcagatg gcaaaaaagc cctccagcac tgaaaggcct
    21361 tatatcggaa acttcccgca acacgggaaa cagacaaatg caaaccgtca aacctcgcca
    21421 acaggaatcg aacctgtatt ttacgcttag gaggcatacg ttctatccgt tgaactatgg
    21481 cgagccgaaa tgaaaaggga gattttaaac ctttccggca acaaaagaca agatgtcggc
    21541 cgcaatcagc ggcccgacag ggataccgcc cataaatgcc accccgatga ccgtaccgat
    21601 cagcaagccc gttaccaata ccggctgccg tcccatcaaa ggcacgccgc atcccgccag
    21661 ccaagccacg aaaataccga taaaaacggc ggatatcatt ttaaaattca aaaattcggc
    21721 aacaggagga acctgcgcct ttcctgaaac caacggactc aaaaccccta tggtcaaaag
    21781 aatgataccg aggttcaacc cgtgcttctc gaccaacggg acaaactgta ccaatgccgt
    21841 ctgctgcatc agcagcaata tggttgccga gacggtaatc gaattgttgt tgctgaccac
    21901 ccccaacaga atcagcgtaa ccagaaacag gggaacaaaa ctgaaattca tcggttacga
    21961 caccctgacc aaagattcct tcgccagctg aatcatagca tccttcactt cgctgtcgga
    22021 cagggcatcc aacgcggcaa cggcacaatc gactgctttg cgcgcctcgc ctatcgaata
    22081 tgccaaagca tccgaacgga cgacataatc gcggattttt tcaaaatagc cgcgatctgc
    22141 attttccaaa gcagtacgca catcgttcgc aacctgttcg gaaccctgac gcatcagata
    22201 aatcaaaggc agggtcggtt ttccttccgc caaatcgtcg ccgacgtttt tacctgtttc
    22261 ttcggtttcc cccgaataat ccagcacatc gtcaataatc tggaatgccg taccgacgta
    22321 cataccgtag tctttcaagg cctgttcgtg cccgggggaa gctttgccca aaattgcgcc
    22381 gacttgagcg gcagcttcaa acaattttgc cgttttatat tggatgactc ggatatattg
    22441 ttcttcggta atgtccgtat tgccgatgtt catcagctgc atgacctctc cctcggcaat
    22501 gatgttggtc gcatccgcca tcacttccaa aacgcgcata ctgcccgagg caaccatcag
    22561 ttgaaatgca cgggtatata aaaagtcgcc aaccaacact gccgccgcat tgccgaacag
    22621 attgtttgcc gttgcccgcc cacggcgcaa atcgctttca tcgacgacat cgtcgtgcag
    22681 gagtgtggaa gtgtggataa actcgaccat cgctgccagc gaatacagtt tctcgtcatc
    22741 ataaccgacc gccttacccg ccaaaatcgt cataatcgga cgcaggcgtt tgccgcccgc
    22801 gctgatgata tatgtaccga tttgcgaaat cagtgcgaca tcggattgca ccgcacggtt
    22861 gatgacttca ttgactttgg caaggtcttc aggcagatgt cgctggaaat agggcagatt
    22921 ctcgagcatg gcagactctc aggtttacgt ttccgtcaaa cggcagatac cgcttgaaag
    22981 ctgattcaaa tagcgggcaa ttatatcagc aaagccatca gggtacatct tttcagacgg
    23041 catatgattg cccgaaccgc gcaaactttg acaaaacagg caaataacaa tacaatgccc
    23101 aatttcgcac aatggtgtgc ggatattaac cataaccctt atggagttga gtatgtacgc
    23161 ggtcgtaaaa accggcggca aacagtataa agtttccgtc ggcgaaaaat tgaaagtaga
    23221 acagatacca gcccaactcg acagccaaat cgaactgacc gaagttttga tgattgctga
    23281 cggcgaatct gtaaaagttg gcgcaccctt tatcgaaggt gcaaaagtaa cggctaaagt
    23341 agtggcacac ggtcgtggcg aaaaagtccg catcttcaaa atgcgccgcc gcaaacacta
    23401 ccaaaaacgc caaggccacc gccaaaattt cacccaaatc gaaatcgtgg caatcgccta
    23461 accttaaaac ttaggagcat tacaaatggc aagtaaaaaa gcaggcggca gcacccgcaa
    23521 cggtcgcgat tcagaagcca aacgcttggg cgttaaagcc tacggcaacg agctgattcc
    23581 cgcaggttcc atcatcgtac gccaacgcgg taccaaattc cacgcaggcg acaacgtagg
    23641 tatgggcaaa gaccacactt tgttcgccaa aattgacggt tatgtcgaat tcaaaaccaa
    23701 aggcgcgctg aaccgtaaaa ctgtcagcat ccgtccttac accggttctg aagaataatt
    23761 caaccgatac ctgaagccgc atcttttcac gatgcggctt tttacatacc ccatacccga
    23821 tttgatacac attaagctga aagtaaaaat ccgcatacac cctcccctgc atatttcttc
    23881 aacaacgggt tttgatataa tcgcctatct gttacagata gttcaaacgg cattcaaacc
    23941 cttacaaatg ccgtctgaaa tcatcggcat ccgcctatgc aaaggatatt ttatgagttt
    24001 acacagcgac atcctcgtcg tcggcgcagg cccggcgggt ttaagttttg cagccgaact
    24061 tgccggcagc ggtttgaaag ttactctgat cgaacgcagc ccactgactg ttctgcaaaa
    24121 tcctccctat gacggacgcg aaatcgccct gacccatttt tcccgcgaaa tcatgcagcg
    24181 tttgggtatg tgggacaaaa ttcccgaaaa cgaaatctac cccttgcgcg atgccaaagt
    24241 gctgaacgga cgttccgact accagctcca ctttccccaa ccgactgaag cgcgcggcga
    24301 gcctgccgac tgtttgggct atctgatttc caaccacaat atccgccgcg ccgcctatga
    24361 agtcgtatca caactcgaca atgtcagcat tctgaccgat acggccgtta aagaagtcaa
    24421 aacatctgat aatgaagcgc aagtcttttt ggaaaatgga gacattctaa ccgcgcgtct
    24481 ccttttggca gcagacagcc gtttctcaca aacccgccga caactcggta tttcttcaga
    24541 tatgcacgac tacagccgaa ccatgttcgt ctgccgcatg aaacataccc tttccaacca
    24601 acataccgct tacgaatgct tccattacgg acgcaccatc gcactgctgc cactggaaaa
    24661 gcgcctgacc aataccgtca ttacagtcga taccgataaa atcaacagcg ttcaaaacct
    24721 ttcgcccgaa gaattggcgg caagcgtaaa agaacagctc aagggcaggc tcggcgacat
    24781 ggaactggtc agcagcatcc accattaccc ccttgtcggc atgattgcca aacgtttcta
    24841 cggcaaacgc agcgcgctga tcggcgatgc ggctgtcggt atgcaccccg taacggcaca
    24901 cggcttcaac ctcgggcttt ccagcgcgga tattctggca aaactgatac tcgaagccga
    24961 acagcgcggt caagacatcg gcgcggcttc cttgttggaa aaatacagca gcaaacatat
    25021 gctgcacgcc catccgcttt atcacggcac caatatgatg ctgaaactgt ttaccaacga
    25081 aaccgcgccg gcaaaactgt tgcgcggttt ggtattacga gccggcaaca acttcccgcc
    25141 gctgaaaaag ctgattacca aacaactgac cggttgaaaa ctgccgaaat gccgtctgaa
    25201 acgtttcaga cggcattttt gtattatgaa ccgttttgcg gtttccatat ccttggcaga
    25261 cggtttgcag cagattcgca tacggcagat gtttcaagca gaaaggaaac acaataaaaa
    25321 taaaaaagcc tccgaacagt cggaggcttt gctttcaaac tgaaattatt tcagtttggt
    25381 ttctttgtac actacgtgtt tgcgggcaac cggatcgaat tttttgattt ccagtttgcc
    25441 gggcatagtg cgtttatttt tggtagtggt gtagaagtgg ccagtacctg caccggattc
    25501 cagtttgatt ttatcgcgca ttgcagtaat ccttaattaa atagtgttta aattaagctt
    25561 cgccgcgagc acgcaaatca gccaatacga catcaatgcc tactttgtcg atggtacgca
    25621 atgcagcgtt ggaaacgcgc aggcgaaccc agcggttttc actttctacc caaaaacgac
    25681 gtgattgcaa gttgggcaaa aaacggcgtt tggttttgtt gttggcgtgc gatacgttgt
    25741 tgccggacat cgggcgttta ccggtcactt tgcaaactcg tgccataatc agtctccaaa
    25801 cttctaaaat agtaaaacgc gatttatacc acgaaaaaca atgccgtttc aagtgtttta
    25861 aagaaaaaca gatgttttgc gcgggtaagc cgcccgagtg ccgcatctgc ggaacgtttg
    25921 caggtgcgaa acagatgggg cgcgattata gcggatttcg ggagcttctg cattcaaaat
    25981 gccgtccgaa catatttcca gtattttcag acggcatttc aagttttcaa atccctcaat
    26041 gtccaccgcc gccgctgtgg aacggcggtt tcgccagcca gacaatggga atcaggacga
    26101 taaacaaaat gctgccggcg aggaatattt cgttcgatcc gatgatgaag ccctgttgcg
    26161 taatggtatt gttgatgatg cctagagttt gaccgtcgga aatgccttgt tgggacaaat
    26221 gcgccgccgt ttcgtgcaat gttgcggaat agggcgtgat gtgttcggca aaacgcgtgt
    26281 ggtgcaacgc ttcgcgccgt tcccacaggg tgctgacgac ggatacgccg acaccgccca
    26341 tcagcacgcg caggaaattc gacaggctgc cggcggcggc aatctgtccg cccttcatgt
    26401 gcgacagtgt gatggtggtc agcggcagga agaacatggc aacgcctacc ccctgccaaa
    26461 actgcggcca gatgacgttg ccgatatcca tgtcggcata aaaatcagtg cgccaataaa
    26521 aagtgaaggc aaaggtcagg aagctggcgg ttacgagcag gcgcatatcg attttattgc
    26581 cgaacctgcc gattaacgga gataggaaga cggggaggat gccgacgggc gcggcagcaa
    26641 gccctgccca cgcggaggta tagcccaagt tggtctgcaa cactaacggc agcagggtca
    26701 gcgtccccat atacaccatg aaacccaacg aggtggcaat tacgccgacg gtaaaattcc
    26761 gatctttaaa cagcgataaa tcgacaatcg gatatttttc tcccaattcc caaacaataa
    26821 aatacgacaa gcacaccagc gcggttatgc ccaaggtaat gatttctcca gaggcgaacc
    26881 agtcaagttc cttacccctg tccagcatca tctgtaacgc gccgataccg accatcatca
    26941 gcgtcagccc gacatagtcg gtcggcgttc tgacggttgc cgtttcgcgg tgtttcaaat
    27001 gtttccatgt aatccatgcc gatatgatcc cgatggggat attaatgaag aaaatccaac
    27061 cccaatgcca gtttccggaa atccaaccgc cgagtatcgg cccgaggaca ggggcgacaa
    27121 cgacggtcat tgcccacaat gccagtgcca gcatccgttt tgcgggcgga taggatgcca
    27181 ttaacaggct ttgcgacagg ggaatcaacg gcccggcgat aaagccctgc aaaatgcgga
    27241 aaacaaccag cgactgaagg ttgggcgcga tgccgcacag ccacgatgcg atgacgaaac
    27301 cggcggcggc ggcggtaaac aatttgacct cgccgatacg ttttgccaaa aagcccgtca
    27361 gcggcacgga aacggcgttt gccacggaaa aagaagtgat gacccacgtc ccctgcgtgg
    27421 ttgccgcacc gaggttgccg gcgatgacgg ggacggcgac gttggcgatg gtcgtatcta
    27481 aaacctccat aaatacggca agccccaaag acagcgtaac ccacgccaat gccgcaccct
    27541 taagcggtgg ataatccatt gtgtcctccg tttcagacgg catcagggcg cggattgccc
    27601 gaggatttca tcgaccgtcc gatcgacttc cgaccagtcg gtactttcca tttccggcaa
    27661 tgccgcaccc ggcgtttttg aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt
    27721 catcgacaaa ccgatacgca acggatgcct gtccacatct tcgcggttca gcacgatacg
    27781 gacggggacg cgctgcacca ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag
    27841 cgaaaacgcg ctgcccgtac ctgccgaaaa ccctgccacc ctgccgcgat aaacaatttg
    27901 tttgccgtac aaatcggaca ccagctcggc aggctgtccg attttcatat gccgcaactg
    27961 cgtctcttta aaattggcat ccacccacac atccgacagc ggcaccaccg ccatcagcgg
    28021 cgcgcctgcc gccacctgct gcccgacctg caccgaacgc ttcgccacct gaccgtccgc
    28081 cggcgcgcgg acttgcgtcc gccgaaggtt cagccacgca tctttcaacc tgccgattgc
    28141 cgtctgaacc tccggctgtt cgcgcaaaga aacatctccg cccaaagccg cgcgtgccga
    28201 agattcttcc gccaaagccg ccttaaccgc cgcctgcgcc tgagacactg ccgtacgggc
    28261 gtgtgccagc tcttcggcgg acaccgcacc cgattccgcc aaagcggacc ggcggcgcaa
    28321 atcatcctgc gcgcgcgcca aatccgcacg gcgcaaggca acctgcgccc ccgcctgcga
    28381 agtggcggca ttttgccggc ggttttggcg caccgcctga accaactcgt tttttgcccg
    28441 ctcgtaagcc agcacatcat tatcgtcatc caacaccgcc agcacgtcgc cttttttcac
    28501 ggcatccgta tcgtcgtgca aaaccttccg caccgtgccg cccttttgcg gcgtaacctg
    28561 aaccacgcgt ccggcaacat aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa
    28621 aaacgccgat ccggcggctg cggcggaaag cgcgaacagc agcgtcaatg ccgtcaggcg
    28681 gcgtttgcgt ttgacttgcg tgttttgaag ttttgtttcg tctgtgtgcg tttccataag
    28741 attgggttcc gaatttcctg atttcatata aacaatattc tttcaatagg aaatagattt
    28801 tatatccgcg caataacgca atcaagtcag tattttagat gcaatattca aaaaacaacg
    28861 cttcaggcgg caaaactgaa aatatatttc gtttatatat gacatggcaa acaccgccgc
    28921 ccgcaccatg ccgtctgaag aaaactacac agataccgcc gcttatatta caatcgccgc
    28981 cccatggttc gaaaacctcc cacactaaaa aactaaggaa accctatgtc ccgcaacaac
    29041 gaagagctgc aaggcatctc tcttttgggt aatcaaaaaa cccaatatcc gaccggctac
    29101 gcgcctgaaa ttctcgaagc attcgacaac aaacatcccg acaacgacta tttcgtcaaa
    29161 ttcgtctgcc ccgaattcac cagcctctgc ccgatgaccg ggcagcccga cttcgccacc
    29221 atcgtcatcc gctatatccc gcacatcaaa atggtggaaa gcaaatccct gaaactctac
    29281 ctcttcagct tccgcaacca cggcgatttt catgaagact gcgtcaacat catcatgaaa
    29341 gacctcatcg ccctgatgga tccgaaatac atcgaagtgt tcggcgagtt cacaccgcgc
    29401 ggcggcatcg ccgttcatcc gtttgccaac tacggcaaag caggcacaga gtttgaagca
    29461 ttggcacgca aacgcctgtt cgagcacgac gcacaataaa ccatccttcc catatttcag
    29521 acggcattcc ttcgatacgg tatgccgtct gaacgccttt ccgaaaggtt tttatgtacg
    29581 cattgaccgc cgcacagcaa cagaaggcac tcttccggct ggtgcttttc catatcctca
    29641 tcatcgccgc cagcaactat ctggtgcagt tccccttccg gattttcggc atccacacca
    29701 cttggggcgc gttttccttt cccttcatct tcctcgccac cgacctgacc gtccgcattt
    29761 tcggttcgca cttggcgcgg cggattatct tttgggtgat gttccccgcc cttttgcttt
    29821 cctacgtctt ttccgttttg ttccacaacg gcagttggac gggcttgggc gcgctgtccc
    29881 aattcaacac ctttgtcgga cgcatcgcgc tggcaagttt tgccgcctac gcgctcggac
    29941 aaatccttga tattttcgta ttcgacaaat tacgccgtct gaaagcgtgg tggattgccc
    30001 cggccgcatc aaccgtcatc ggcaatgcac tggacacgtt agtatttttt gccgttgcct
    30061 tttacgcaag cagcgatgaa tttatggcgg caaactggca gggcatcgct tttgtcgatt
    30121 acctgttcaa acttaccgtc tgcaccctct tcttcctgcc cgcctacggc gtgatactga
    30181 atctgctgac gaaaaaactg acggccctgc aaaccaaaca ggcgcaagac cgccccgtgc
    30241 cctcgctgca aaatccgtaa aacagctctg tagggaatcg ggacggttgg gttttctttt
    30301 atttgcaaac actgccggaa cggataaatc cggattccca cccacttcgt cattcccgcg
    30361 aaagcgggaa tccggtgcgt tggggttcag tcatttccga taaactcctg ccgcattgta
    30421 tttctagatt cccgctttcg cgggaatgac ggcggatggg tttccgtgcc gtggtgtccg
    30481 gattcccgcc ggtacgggaa tgacggcagc gcttccttgg cttagtttta ttttaagcca
    30541 ctataaatgc cgtctgaaaa cctgtttgca gattttcaga cggcattgtt ttcgtcgccg
    30601 gttcaaactt caatccgatt gcgacacggt aaccggtatg ccgcgcaagc gcgcaagcag
    30661 cgcgtccgcc ttctgtttct gcgcggcaaa cgccgccttg tccgcctgcg tcaattcggg
    30721 tgtcggcaat gcgaccgaaa caggattgac gggctgcccg ttgatgcgcg cctcgtaatg
    30781 caggtgcggc ccggtcgaac gccctgtcga accgacaaaa ccgatgacct cgccgccgcg
    30841 cacattgcct tgtgcctgcg aaaacgcgct caagtgcgcg tacagcgttt ccacaccgtt
    30901 ggcgtggcgt atcatcaccg cgttgccgta tccgcccttc cggcctttaa aggtaatcac
    30961 gccgtcggcg gaagccctga ccggcgttcc ctgcggtgcg gcataatcga tgcccgtgtg
    31021 cagccgccat gtgtgcagga tggggtgcat acggtagccg aacggcgaag aaatgcgcgt
    31081 atagaccagc ggctcgatgt tgaagccgcc tttttcctgc aacaccctgc cgtcttcatc
    31141 gtaataattg ccgccccctc cgccttcctt gtccgaacgg taatagaacg cctgatgggt
    31201 tgtgccgccc ttgacaactt ccgccgccaa aatatcgccc gccgccacct gctgcccgtg
    31261 gaaatacagg ctgtcgtaaa gcaggcgcac ggcatcgcct tccttcaaac cgtcaaggct
    31321 gaagcggccg gcaaaaatcc cgcttaagga ttcgcggatt tcgacgggca cttccgcccg
    31381 cgccagcgaa ccgcgcgccg acgttttgac cacgaccgaa cgcagcgtcg gcaaaacctt
    31441 catatccgca tcagaagccg accgccgcca tatgccgcct tttttttcca aagcgaccag
    31501 attgcgctcg ccgtcttcgt cggtaaaaaa ctgcacttcg cgcgcactgc cgtcgccgcc
    31561 gaccaaaaca tgaaccgact ggtcggcacg caaatgccgc aaatcggctt cgccgccata
    31621 tttttccgtg attcgggcaa tctcgtcccg cgccataccc gaacgcgcca gcacgtccgc
    31681 cagcgagtcg cccggctgca ccgcctcctg cacccaatat gccgtctgaa cgccgctgcc
    31741 gccccaagac agcggcggca gtttttgttc cacgcgctgc ggtctgacgc gctccgtccc
    31801 ctctgtcgaa gcaatgtatg ccgccgacac cagaataatc gaaacggcaa gcgcgcgcag
    31861 cgcgtatttc cgatgttttg ccgaaagtgg gaagacagcc atattgttcc tttttgacgg
    31921 cgtttgcgcc gaatcctcta taatatccgc ccgatcctca tttgtcaggc atcaaacgat
    31981 gaaactcacc ctcgccctcc cctcgctcaa tttggatgaa gacgaaatcc gaatacctct
    32041 atgcctgccc gccttcaaca aaatcctgca atacggctcg ccgcaccgcc aaagctgcac
    32101 ggcttcggca ttttacgcac gttatttgtg gtgcggaaga ctggcacaac gcccggcgca
    32161 aagcctgaat atgccgtctg aaaccgtcgc gctcgccacg cccgtctggc aaaaaatggg
    32221 gctgcatcag gcaaatgtgc tgacggcgga atatttggat gtcggtacgg atgaagcgga
    32281 acggctctgc cgcgatctgt cggcgtttta cggcgacatt ccgtggcgtt tcgttcccgt
    32341 cctgcccgaa ttatggctgg tttcccttcc ccgcgcgtac cgctggggcg cgaagcccgt
    32401 tttggacttg ggcggactgt tgggcgcgga cgatcagccg gacggggaag acgcgttgga
    32461 atggctgcgc gtccaaaccg aaatccaaat gtggctgaac gctcaccccg tcaaccacaa
    32521 ccgcaaaaaa cgcgggctgc ccgaattgaa cggcttatgg ctttgggaca gtctgcacgg
    32581 aagcgcacaa ggcggcacgc ttttcgccga tacggtttgg agccgttttc acccgaaccg
    32641 ccgagcattg cccgacagct tccgagctta tgcggaaacg gcggcacacc tgcccgatac
    32701 gcaccacatc ctgtttatgg acgatttgcg cctgaccgcc ctgacagggg acagggagcg
    32761 gtatgcggca atattgcagc agtggggaga acgctggttc gcaccgcttt acgaagccgt
    32821 ccgcaccggc aaaatcaaac ggctggacat cgccacggac ggacaacacg gcggtacgct
    32881 gaccttcaaa cccacagaca ggcggaagtt ttggcgctgc acgaaaacct ttgacggaat
    32941 ctggtaaacc cgcccgccgc aaccgcgaca atgccgtctg aagcccgaat cgggtttcag
    33001 acggcatttt accccgcatc gccgttatgc cggaaaaacc ggaatacgtt tccgcacacg
    33061 cggcggcacg caaaacaccg cccgaaagcc gccgatgccc gccgtgacac cgccgccggc
    33121 agggtttccg ccgaaaagga acagcaatca gaacgttttg ttaaactcga caaacgccct
    33181 gtttttctcg tattcgttaa acacatcgtt gctccacgtt tcgcggtgcg acagcgtcag
    33241 gcgcggcgtg atgcctttga aatgcaatgc ccggtgccaa aggctcaagg atgtgtccga
    33301 ttctttatcc ctgcgccttt cccctttaaa actgctgaag aagccgggtt tttcataatg
    33361 ccgtttcgcc acgccgaggc ggaacagcga agacaggccg ctgccgcccc attcctgccc
    33421 ccaggcaaag cgcaggccgt aacggttgaa attgtcgcca cggtcggcgg ggttgcgctc
    33481 gcggtaaaaa tccaaaccgc ccgtccaata ttggcgcgca ttccggtaaa acaccagcga
    33541 attggaaatt tgcaaatggg tattgtcgga acgcgcccgg cgcgtattct tcaaacgccc
    33601 ccactccgcc gaagacagcg tttgccatct cggggtttgc caacggttga aataaaggcg
    33661 tgcgccgttg gcgtaagaat aagcgtcgtt gccgtaggtg cggcgttcgt ggaacactgc
    33721 cagcccgaca tctttacgcc ggtcggcaaa accgatgccg ccggaaacac ctgccgtcat
    33781 atcgttgaat ttcttattcc ccggataagc cctgccggac acgtcgccgc ccgccgtcgt
    33841 gtaccagccg tttttcagcg accatttttt ctccgcgccg aaccggtaat tgaccgccgt
    33901 gccgtccacc tgtttcggga aagtccagtt gccgtactgc tgctgtttcg gggcttggtt
    33961 gatattgtgt tcgcgggtaa tgctgaaacc gccgtttacc ttccacgcat cgcgttcgcg
    34021 caatgccttg cggtacagct cgacctgctc cataagctgc ggcggcagat cttctgtttt
    34081 caggcggtcg aactggtctg ccgccgcctc gttctgcctg tcttcaaaca atgccgccgc
    34141 caaacgcata cggacggcgg gcgcgtcggg ttgggcggca atcaattccc ggtaatggga
    34201 aaccgcctcc ttcaccctgc cctctgcctg cgccaaaatc ccttgtgcat aaagtgccaa
    34261 catcttatcc tgccgcgcct gttgtaggta aatcggcaaa atcacgcgga taccggcaat
    34321 attgtttgag accactgcgg aatacatggc acgcgacaac aattcgggat ttttcagcag
    34381 ggtttcgccg tcaacctgca aaacttttcc tttttcccgc acctgccccg gtaccttctc
    34441 cctgtcgatc ggtttgacct ccgcttcatg aagcctgaac tcgggacggc ttctcaaatc
    34501 cggttcgcgc ggtgtttctt cggcgtatgc cgcgcttgcc aataaaggca gcagcatcca
    34561 tctgtttcgt gcaggcataa atgtcttccc acaaaaataa aaataaaaaa taaccattat
    34621 caaccggcgg attgtatcag ttttttatgc cgctgttcca aaccctgccg cccgatgccg
    34681 cgacaatgcc gtctgaagcc agaatcgggt ttcagacggc ggcaaaaaac cggcggtttt
    34741 ccgccggttt catcattatg ccccgccctt cggggcggca gggttcggat cggtttgcgg
    34801 ggcggcgcac ggggccgtgc aggtgtgccg cctgtgtgtc aaaacatttc ccggcgcatc
    34861 aaaacaacca tttgaccgac aatgcggctt ctttgtcgcc gtccgtcctt ttgccgtatc
    34921 cgatgcgtgc ggacatatgg cctttccaac cggcttcaat gccaaaccgc ccttcgagcg
    34981 ccgtcctgcc tgccagcgtc tgtttttcgc cgtccatttc catgccgaaa gattttgacc
    35041 tgtgcaaaac attaaaagcg gcaaaaggct gaagattgac accgttacgc aaagcaaaac
    35101 gggtttttgc ccgaatgccg gcgcggattt gccactgacc gctgccgagc agcccgaccg
    35161 ccgcccctcg ctgtcggtaa agccgccgtt tacgcccaag taggtaaact gcgcctgcgg
    35221 ttgcaggtaa aactgcatat tactgcctta tatattgcgt ccctaagaag gggcgattaa
    35281 caaaaattaa cgccccttct tagggacgca atatataagg agtgagtacc tgttcttcta
    35341 ttaaagaatt aatcttttga ggcattttct gttgaagttt attaaacaca tcttcagaaa
    35401 ttccaaactg ctttattatc tcttccctaa cccgtttttt attttcttcc caagcctcgg
    35461 gtcttgtttt gtataaatcc tgtaattgct ttttaactgc ctgctttaaa tcagttcggt
    35521 aattatctgt aatatatttc ttaacaacag gcatattatc gacggcgagg atgccgctat
    35581 tgtagttttt acccgtaata ttgatttcgt catacaaggc ttcagatgat ttttgatcgg
    35641 ttttaccgct ggagaaataa tggaaaacac cgtttgccgg cttgtctgaa aatgaaaaat
    35701 cggcagtatc gccaacattt gtaggacgag tatccgaacc gggctgataa ctgaaagcac
    35761 cgaagcaatc aaaagacaca ctgcacgggc cgcgccaaac gtgggaattt ctgtcttccg
    35821 ccgcttctaa aataaatttg tttttaatct cggcttcttt gacgggatcg tacgcatccg
    35881 aaccccaagc atatggcgtg caaaacagcg ataaaacaga taatgttaat agattaaccc
    35941 gaccgcttgc ttgcttgctt gcttgcttgc ttgcttgctt gcttgctttc agtgtcatga
    36001 gaaattttcc tttgtcaagt gttaaagtta taatgattat atactatatc acactacatc
    36061 gcaatgaaaa atcgggaaaa acaaaaaacc cctccgccgt cattcccgcg aaagcgggaa
    36121 tctagaattt taatgcggca agaatttatc ggggatggcc ggggtttaaa ggtctgggtt
    36181 cccgcctgcg cgggaatgac ggtttagaaa tcacccgaaa cgagaaaaag aaccgaaacc
    36241 ggacaagtcg gattcccgcg cgggcgggaa tgacgataag cgtaatttca atccgccata
    36301 ttgtcttttc acttgcctga aaacagattt cagccgccta tccctgcatc cggcggctga
    36361 tgtagtcggc aatcaaatcc cgcttttccc aaaaaaactt ctgcattttt tgttcggaat
    36421 acagtttgcg gaccagagtg ggcagaccgc tgtggacgat ggcggcgatt tctttgttgc
    36481 cttcttcttc ctgcattgcc gtctgaaaat ccaaacccct gcctttcaga tatgctgcaa
    36541 ggcggttgcg gatgtgtgaa ttgagttcga tggatttgag cattttgcct ccgttttgcg
    36601 gtaaggattt attcgatttg tttatccgcc tgattttatc acgtttccca agcggtttgc
    36661 ctgtacaata ccgcttttcg gaagggtgtg ggcgatgatg ttttcttggt tcaagctgtt
    36721 tcacttgttt tttgtcattt cgtggtttgc agggctgttt tacctgccga ggattttcgt
    36781 caatatggcg atgattgatg cgccgcgcgg caatcccgag tatgtgcgcc tgtcggggat
    36841 ggcggtgcgg ttgtaccgtt ttatgtcgcc tttgggtttc ggcgcggtcg tgttcggcgc
    36901 ggcgataccg tttgccgccg gccggtgggg cagcggctgg gttcacgtca aactgtgttt
    36961 gggcttgatg ctcttggctt atcagttgta ttgcggcgtg ctgctgcgcc gttttcagga
    37021 ttacagcaat gctttttcac accgctggta ccgcgtgttc aacgaaatcc ccgtgctgct
    37081 gatggttgcc gcgctgtatc tggtcgtgtt caaaccgttt tgacggagca tcttatgccg
    37141 atagaaatcg aacgccgttt tttgattgaa aacgacaaat ggcggcaata cgccgatgag
    37201 ccgctactgc tgaaacaggg ctacctgtct gttgaaaaag aacgcaccat ccgcatccgc
    37261 attgccggaa aacgggcgtg gctgacgctg aaaggctata tttcggaaat cagccgcagc
    37321 gagttcgaat acgaaatccc gcttgccgat gcggaaaaga tgatggaaac gatgtgcccg
    37381 tttaagatgg aaaaacggcg ttatccggtc agatggggcg gcagcctgtt tgaagtcgat
    37441 gttttccttg gcgaaaactc gcctttggtc gtcgcggaaa tcgagttgcc cgccgaaaat
    37501 gccgattttg accgtccgga ctggctgggg cgcgaaatca cttcagacgg tatgtttacc
    37561 aatgcctatt tgagcaaaca tccgttctca agctggaaaa atgccgtctg aagcggcttc
    37621 agacggcatc tgccttgcgg ttatcccttg aggtaatgca cgaatgtata tgcaacgcct
    37681 ttgctgctga cacggcgttc cgtccgctct gcttctctcc aatgcgtccg gtctatttct
    37741 gggaaaaatg catctccttc cacagacaaa tccacttcgg ttatccgcaa atcggtcgct
    37801 aatggcatcg cttgtccgta tatctgcgcg ccgcccataa tgacggcttc ttccgcgccg
    37861 gcgcacaatg ccaatgccac ctccaaactt gccaccgttt ccgcgcctgc cgcgcaataa
    37921 tccgcctgcc ggctgatgac gatgttcctc cgtccgggca ggggtttgac gggcagggat
    37981 tcccacgttt tccgccccat aatgacgggt ttgcccaagg tataggcttt gaaaaatgcg
    38041 aaatcttcgg ggatgtgcca aggcatagca ttgcccgccc cgatgcacag gttttccgca
    38101 cacgccgcaa ttatggttat tttgagcata ttttctccac aaaaatgccg tctgaacgct
    38161 tcagacggca ttttatgaca ttctgcgtca aaaatctcaa cctgcgcgcg ccatacgttt
    38221 tgtgttcgca cccgccaaca atgccaacaa ttcttcagtc gcgccccaac cgatacacgc
    38281 atcggtaatg ctcttgccgt acacttccgg cttgtcctgc ctgccttcga ccaaatggct
    38341 ttccaccatc acgcccatga tattgccgcc gtcctgttcc aattggtcgg caatgtcttg
    38401 tgccacttcc atctgccggg tgtaatcctt gcggctgttg gcgtggctgc aatcgatcat
    38461 cagcttgtcg gttacccctg ccgcacgcag ttgttccacc gcctcgctga cgtgtcccgc
    38521 atcataattc ggctcttttc cgccgcgcaa aatgacatga cagtcgggat tgccgccggt
    38581 atggacaatg gcggaatgcc cggccttggt tacagacaag aaatgatgcg aatggctcgc
    38641 cgcaccgatt gcgtcgatcg caatcttcaa attgccgtcc gtaccgtttt taaagccgac
    38701 ggggcaggac agaccgcttg ccaattcgcg gtggacttgg ctttcggtcg tccgcgcgcc
    38761 gattgccccc caagaaatca ggtcggcata atactggggt gtaatcatat ccaaaaattc
    38821 ggtagaggca ggcatgccca tattattcag ggacaacaac agactgcgcg cctgacgcaa
    38881 accgaaattg atgtcgaacg taccgtccaa atgcgggtcg ttaatcaaac ctttccaacc
    38941 caccgtcgtt ctcggcttct cgaaataaac gcgcatcacg atcaaaagct cgttttcata
    39001 ccgcttgcgg agtttcaaca gacgttccgc atattccaac gccgctttcg gatcgtgaat
    39061 cgaacacggc ccgataatga ccaacagccg cctgtccctg ccgtgaacca aatcggaaat
    39121 ttcctgacgg gtgcggtgaa ccaagcccga agcctctttg gaaatcggca gctcgtaaag
    39181 atgggcaatc ggcggcaaca actctttaac ttctttaatc ttaatatcgt ctgtggggta
    39241 atggtgtgtc atcttgcatc tttcctgatg ggtttttata cgctgcttag gttacatcgt
    39301 ttaccttgca caaacaagtg caacatatta ctacaaagct attttatttc aaaataactg
    39361 atatatcttt tttcatagat tttaaatttc atatttttaa tattttagga attttatttc
    39421 aaagaaaaag aaacagattg ataaaaacaa tacccgccat tcattcgggc aaaaccgcac
    39481 ggaacatccg ttttcccgtt tccacgacaa tccggtacag gacaccccgc tgccgattcc
    39541 gcctgcacat ccggcaaaat ttcatacgca aggaaaatgt tgaaaacaaa aagaactaat
    39601 tgcttgaaaa aagatttaat tggaatgcgg gtatttccca cactttctcc atttatttat
    39661 cctcattata aaaacatgaa aaaaaacccc tgcttgcact ctcacttgca gccgtgtccg
    39721 ccgttacggt agccgatatt cccgagtcta acggcaaagg cgaatacaaa gtctttaccg
    39781 cttatgttaa aggcaaagcc actgccggca atttgaaaga aaaataacgc acaccgtcca
    39841 tcggttttcg ttcaacgaac gccacgatat gcgttttaag gcagagtacg cccgaagcaa
    39901 aaacgatgca gacgacaaaa gagggaatgc acacatcgct aacggcgcaa taggcaaaat
    39961 ggctgccgtg tatgccggtt atacctacac ccaaccgggc ggcgaatcca cacgtttccg
    40021 tggcggtttg gagcgtgtcc aagttaaacg ttatctggga gaaaaatgct gcggctacgc
    40081 ctgtccaaaa agcgaacgct tcaccctgca agcagatggt gaaatccgtc aaaaccttgg
    40141 cgaaggctgg tttctgaagc tatgagcggg gtggctgtct atctccgcaa caaaaacaga
    40201 atagcaattg cctcccaaga tgccaaccta aactccaaag gccggttcgt cagcagcggt
    40261 ctgaatgtgg gcaaacaact gaccggcagt ctcggcgtag agtttgaccc atactaccgc
    40321 caccgcgcaa tccgcaagtc tgccgaattt gtctcgaaca ctaccaaaac caaaacagac
    40381 agcgaaaagt ttaacgagta gggcttccgc gtagccgcaa cgttctaatt cccttacgta
    40441 taaaaagcag cataaaaatg ctgccttttc ccatttccaa gggaagtatt ccgtagtgca
    40501 gcttacctca ttgtttcaca caaaccgaaa atgccgtctt aaaaccaatt ttcagacggc
    40561 atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca caggttgcct
    40621 tccacccttc gtacaacctt tgctccatca aacatccact tcctaaatac tgatgtatac
    40681 tg
//
LOCUS       DAXDZG010000008        40136 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXDZG010000008 DAXDZG010000000
VERSION     DAXDZG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40136
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.008"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..288
                     /locus_tag="ACFB78_000364"
     CDS             <1..288
                     /locus_tag="ACFB78_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC1268442.1"
                     /translation="VWDDEEYWKLENALIEVRRKYPYPMDIPRDIVIGIGIIIDFLMV
                     PNWELFEIKASPWLPDSVGIHERYERFRVMLRYIFTEIDIVNVRFDYYNKK"
     gene            complement(352..2565)
                     /locus_tag="ACFB78_000365"
     CDS             complement(352..2565)
                     /locus_tag="ACFB78_000365"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC1268443.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            2708..3349
                     /locus_tag="ACFB78_000366"
     CDS             2708..3349
                     /locus_tag="ACFB78_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC1268444.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(3421..4059)
                     /locus_tag="ACFB78_000367"
     CDS             complement(3421..4059)
                     /locus_tag="ACFB78_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC1268445.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            4315..5166
                     /locus_tag="ACFB78_000368"
     CDS             4315..5166
                     /locus_tag="ACFB78_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC1268446.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            5232..5894
                     /gene="exbB"
                     /locus_tag="ACFB78_000369"
     CDS             5232..5894
                     /gene="exbB"
                     /locus_tag="ACFB78_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC1268447.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5897..6331
                     /locus_tag="ACFB78_000370"
     CDS             5897..6331
                     /locus_tag="ACFB78_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC1268448.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6832..7260)
                     /locus_tag="ACFB78_000371"
     CDS             complement(6832..7260)
                     /locus_tag="ACFB78_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC1268449.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(7325..9073)
                     /locus_tag="ACFB78_000372"
     CDS             complement(7325..9073)
                     /locus_tag="ACFB78_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC1268450.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            9399..10832
                     /gene="ccoN"
                     /locus_tag="ACFB78_000373"
     CDS             9399..10832
                     /gene="ccoN"
                     /locus_tag="ACFB78_000373"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC1268451.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10859..11470
                     /gene="ccoO"
                     /locus_tag="ACFB78_000374"
     CDS             10859..11470
                     /gene="ccoO"
                     /locus_tag="ACFB78_000374"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC1268452.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11475..11645
                     /locus_tag="ACFB78_000375"
     CDS             11475..11645
                     /locus_tag="ACFB78_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC1268453.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            11669..12988
                     /gene="ccoP"
                     /locus_tag="ACFB78_000376"
     CDS             11669..12988
                     /gene="ccoP"
                     /locus_tag="ACFB78_000376"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFC1268454.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            13332..14741
                     /locus_tag="ACFB78_000377"
     CDS             13332..14741
                     /locus_tag="ACFB78_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC1268455.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            14842..18051
                     /gene="recC"
                     /locus_tag="ACFB78_000378"
     CDS             14842..18051
                     /gene="recC"
                     /locus_tag="ACFB78_000378"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC1268456.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSG
                     ELGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVKSGETEVLAEIAQDRALQLLSEWI
                     VFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(18121..19689)
                     /gene="mtrF"
                     /locus_tag="ACFB78_000379"
     CDS             complement(18121..19689)
                     /gene="mtrF"
                     /locus_tag="ACFB78_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC1268457.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPVP"
     gene            20021..20233
                     /locus_tag="ACFB78_000380"
     CDS             20021..20233
                     /locus_tag="ACFB78_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268458.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(20326..20958)
                     /gene="mtrR"
                     /locus_tag="ACFB78_000381"
     CDS             complement(20326..20958)
                     /gene="mtrR"
                     /locus_tag="ACFB78_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC1268459.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            21208..22446
                     /gene="mtrC"
                     /locus_tag="ACFB78_000382"
     CDS             21208..22446
                     /gene="mtrC"
                     /locus_tag="ACFB78_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC1268460.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGRDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            22458..25661
                     /gene="mtrD"
                     /locus_tag="ACFB78_000383"
     CDS             22458..25661
                     /gene="mtrD"
                     /locus_tag="ACFB78_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC1268461.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMTLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            25714..27117
                     /gene="mtrE"
                     /locus_tag="ACFB78_000384"
     CDS             25714..27117
                     /gene="mtrE"
                     /locus_tag="ACFB78_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC1268462.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERISYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(27318..27510)
                     /locus_tag="ACFB78_000385"
                     /pseudo
     CDS             complement(27318..27510)
                     /locus_tag="ACFB78_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27516..27722)
                     /locus_tag="ACFB78_000386"
                     /pseudo
     CDS             complement(27516..27722)
                     /locus_tag="ACFB78_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            28072..28851
                     /locus_tag="ACFB78_000387"
     CDS             28072..28851
                     /locus_tag="ACFB78_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC1268463.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(28889..28995)
                     /locus_tag="ACFB78_000388"
                     /pseudo
     CDS             complement(28889..28995)
                     /locus_tag="ACFB78_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(29090..30424)
                     /gene="gdhA"
                     /locus_tag="ACFB78_000389"
     CDS             complement(29090..30424)
                     /gene="gdhA"
                     /locus_tag="ACFB78_000389"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC1268464.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            30848..31642
                     /locus_tag="ACFB78_000390"
     CDS             30848..31642
                     /locus_tag="ACFB78_000390"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC1268465.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            31651..32663
                     /locus_tag="ACFB78_000391"
                     /pseudo
     CDS             31651..32663
                     /locus_tag="ACFB78_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(32838..34175)
                     /locus_tag="ACFB78_000392"
     CDS             complement(32838..34175)
                     /locus_tag="ACFB78_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1268466.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            complement(34409..35473)
                     /locus_tag="ACFB78_000393"
     CDS             complement(34409..35473)
                     /locus_tag="ACFB78_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1268467.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(35474..36232)
                     /locus_tag="ACFB78_000394"
     CDS             complement(35474..36232)
                     /locus_tag="ACFB78_000394"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1268468.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(36307..36382)
                     /locus_tag="ACFB78_000395"
     tRNA            complement(36307..36382)
                     /locus_tag="ACFB78_000395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36347..36349),aa:Ile,seq:cat)
     gene            complement(36436..37781)
                     /locus_tag="ACFB78_000396"
                     /pseudo
     CDS             complement(36436..37781)
                     /locus_tag="ACFB78_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757555.1,RefSeq:WP_003777802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            complement(37775..38504)
                     /gene="fabG"
                     /locus_tag="ACFB78_000397"
                     /pseudo
     CDS             complement(37775..38504)
                     /gene="fabG"
                     /locus_tag="ACFB78_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            complement(38530..38966)
                     /locus_tag="ACFB78_000398"
                     /pseudo
     CDS             complement(38530..38966)
                     /locus_tag="ACFB78_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            39230..39979
                     /locus_tag="ACFB78_000399"
     CDS             39230..39979
                     /locus_tag="ACFB78_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFC1268469.1"
                     /translation="MRDMAQWRQWAECPDFADGLPDVRRELPFLPAMRRRRLSKAARL
                     VCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELLKSHTVSPTSFGLSVHNATAGQ
                     WSILRRDMSEQTALAVCADGVETALAEAASLLEEGCGSVLVLAADDPLPEGYAVSATR
                     APFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEAYWSGLEWVRFLLNGSRECRRV
                     YRNREWLWQPASCRLKISAMSAPSTTWYRRY"
BASE COUNT         9355 a         9872 c        11237 g         9672 t
ORIGIN      
        1 gtgtgggatg atgaagaata ttggaaattg gagaatgctt taatcgaggt taggagaaaa
       61 tatccttatc cgatggatat accaagggat attgtgattg gaatcggtat cattattgat
      121 tttttaatgg ttccaaattg ggagcttttt gaaattaaag cttccccttg gttgcctgat
      181 agtgtgggaa ttcatgaacg ttatgaaagg ttcagagtaa tgctccgtta tatttttacc
      241 gagatagaca tagtcaacgt gcgatttgat tattacaaca aaaaatagca aaccccggca
      301 atcaaaatgc cgtctgaagc gttatccggc tttcagacgg catttttgta tttaaagccg
      361 ggtaacgctc aatacgcctt tgacatcgcc gaggccggcg aggacgcgcg ggaggtcgtt
      421 gacttgtttg acttcgagcg tgaacctcat gctggcttcc aagtcgcggg actgggtttg
      481 cacggcggta acgttgagtt tgtggcgggc gagcgcgtcg gatacgtcgc gcaaaagccc
      541 ggagcggtct tgggcgcgga tttcgatatc gacggcgaac acttgccctt cctgcaacgc
      601 cgcccaactt gcgtccagta ctttttcggg cgcgtgttcg gcaaggtgtc ggaaagaggg
      661 gcaggttttg cggtggacgg aaatgccgcg ctcgcgggta acgaagccgg caatatcgtc
      721 gggcggcgcg ggtttgcagc atttggcaag cgtggtcatc aagccgtctt cgccgtcgat
      781 gagcacgccg gttttgccac cttttttgat tttggactgt ttgacgatgg tggttgcgct
      841 gacgggcacg ggcggcggtt cgttcagcgt gccgcaggct ttttggatgg cgcggttgga
      901 aatttcgcct tgtccgacgg cggtgtagag gtcttctggc tttttgtagc cgagattttc
      961 ggcaagctct tgcaggttgg gtttgggcgt gagtttggca agctgcttgt cgagttggac
     1021 acggccttct tcgcgcacgg tgtcggcgtt ttgctggcgg atgtaggcgc ggattttgcc
     1081 gatggccttg ccggatttga cccagccttc gtaaagccag ttgacggaag gatgcccttc
     1141 tttggcggta atgatttcga cgcgctgtcc gttttcgagc ggggtggaca gcggcacaat
     1201 ctgcccttcg actttcgcgc cccggcagcg gtcgccgatg ctgctgtgca gggcgtaggc
     1261 gaagtcgatg ggggttgcgc ccgttggcag agagaggact ttgccgtgcg gggtcaaaac
     1321 ataaatcgtg tcgttgaaaa gctcggtttt gaaggcggcg gcgaggtctt ccttgccgct
     1381 ttccgccata ttttcgcgcc agtccaagag ttggcgcaac caggcgattt tttgttcgta
     1441 ggcggaatcg cctttgccgc cttctttgta acgccagtgg gcggcgacac cgaattcgtt
     1501 gaattggtgc atatcgaagg tgcggatttg cacttccaca cctttgtctt ccgggccgac
     1561 gatgacggtg tgcaaacttt tataaccgtt gcctttgggg ttggcgatgt agtcgtcgaa
     1621 ctcgccggga atgggctgcc agaggctgtg gacgatgccc agcgtggtgt aacactcggg
     1681 gacggtatcg accagaatcc gcacggcgcg gatgtcgaac aggccgtcga agctgagttt
     1741 tttcttcacc atttttttgt aaatggagta gatgtgtttc ggacggccgg cgacttcaaa
     1801 gtggatattg tattttttga gttccgtacg caggatatcg aggaagtttt cgatgtattc
     1861 gaggcgttcg gtgcgttttt cgtccaaaag cagggcgatt tcgcggtatt tttcgggttc
     1921 ttgatggcgg aagcccaaat cttcgagctg ccatttgagc tgccacacgc ccaagcggtt
     1981 ggcgagcggg gcgaagatgt cgagggtttc tttggcgacg gcgcgttttt cagggctgtc
     2041 gggggcgttg cttaaaaatt gcagggtgcg cgtacgcatc gccagtttga ttaatacgac
     2101 gcggatgtcg gtaaccatcg ccagcagcat tttccgcatg gtttccgctt gctgtgcgcg
     2161 ttcttccggc gtggcgaggc tgtccacccg ggcgaagtgg gtaagtttct gcacttcgtc
     2221 cacacctttg accagctcgg cgacggtgct gttgcagcgc tcggaaacca ataggttcca
     2281 atcggggacg tagcgtccga tgtcggcaag cagggtggcg gcgacggcat cgggcagcag
     2341 gtcgagttcg tcgaccattt gcgccgcgcc gaggaagtgg tcgggcagcg gctcgccata
     2401 cggcgtggcg gcatcggcag gataatgttc ctgcgccagc gaccatgcgg taccgatgag
     2461 gtttttatcg ttgtccggca gagcggcgca gtagctgtcg aaccattcgc gcaattcttg
     2521 cagggtcgcg ctttgcgtgt cttgaatcgg gctgatggcg gtcatgatgg gatcttccgt
     2581 tccgataaaa acgagttcat tgtaacagct tatttattta cggtgcatag gcgggaagat
     2641 aaacttgttt cgggcggcgc cgggtgttaa ggtagccggt accgacaggt tgacgaggaa
     2701 tattttgatg aaactgtata tttacgacca ttgcccgttt tgcgtgcgtg cgcggatggc
     2761 tgccgggctg tttggtgctg acgttgaaga agtcgtgctg gcaaacgacg acgaggcaac
     2821 gccgatcggt atgatcggtg cgaaacaagt gcccgtcctg caaaaagaag acggttcgtt
     2881 tatgggcgag agtttggaca ttgtgtgcca tttcgaccga gaaggccgtc tgaaagacga
     2941 agtccgcccc gagatacagg cgtggctgga caaggtaggc ggatacaaca acaaattggt
     3001 tcagccacgc ttgatcaaaa tcgggctgcc cgaatttgta acgccggaag cggtgaaata
     3061 tttcacagac aaaaaagaga aaagcatcgg cagcttttcc gccaatttga ataaaaccgc
     3121 ccaatacctt gaacggataa acgcagattt gcaagagttg gagaatttga tggacggtac
     3181 ttcagacggc atcaacggtg gaatcggtat ggaggatatt ttggttttcc ccgttttgag
     3241 gaatctgacc gtggtgcgcg gcatcgcgtt ccctcggaag acgatggatt atttaatcgg
     3301 tatgagtgaa aaatcaggcg tgccgcttta tttcgacagg gcattgtaaa accaaaaaaa
     3361 agcggcaggg caatatgtcc ttccgcgttt tttggaacgt ttatgccgtc tgagagccag
     3421 tcagacgaac atctgcacaa acgcaatcac cagccgcaca atcggtgcaa taaacgcacc
     3481 caaaaccccg gtcagcataa gcagcaggat aatccacgtc ccataaggtt cgattttgcg
     3541 gaacgcttgc gaatatttcg ccggcaggaa ggtgtcgatg aaaatgccgc cgtcccaagg
     3601 caggatgggg atgatgttga gcgcgaacaa aatcgcattg atcagaatac cgtagtttgc
     3661 catttgagcc agcggcatct gatacgcccc gccggcatac ggagttagca ccaaaaccac
     3721 gccccacaga acagccatcg ccagattcga cagcgggccg gacgcggcaa tgcaacgcca
     3781 tgcaaggcgc gggttgcgga agttgcgcga atcgatagga atcggacgcg cccagccgaa
     3841 caggaagggc gtgaacatca aagtaagcag cggtacgatg attgtgccga ccaaatcgat
     3901 atggggcagg gggttcagtg tcagcctgcc gtattgttcg gcagtgttgt ctccccagta
     3961 gcgcgccgta tagccgcgcg ccacctccct gacggtaatg gcgagcagca cgggcaggac
     4021 agcaagcaga aacacgccca agtcaaaatt ttgaaacatg gttttccttt gaacagatgt
     4081 ggtgtgcagc ccgaaacggc tgacagacag aagcggcaat atatcatcta acctgatttt
     4141 attggaataa gaaatcagac ggaatcattt tttctgccgg cccgttatct gatgatttgc
     4201 tgtaataccg cgtttaaaca cggtttgcct atgaatggag caggcgttca catgacaaca
     4261 aattataaaa aataggttgc aaataggaat tgttgctatt attatgcccg aattatggat
     4321 aaagaacgaa ttttaacccc cgcagtcgtg ttttccgtcg cactgctgca tttggcaata
     4381 gtggcattgc tctggcaggc gcacaagctg cccgtgatag agtcaggaaa tgttatcgaa
     4441 tttgtcgatt tgggcgattt tggcggaggg ggcggcgcac ccgaaggcgc aggcgcgcct
     4501 gccgcgcccg aaccgcaacc cgcgcccgat ccgcccaaac ctgtcgaacc gcccaagccg
     4561 gttttgaagc cggcggttac aaaaaaggcg gatgcggata ttcagcaacc taaggaaaag
     4621 ccgaaacccg aagaaaaacc taagcccgaa cccgaaccgg aagcgaagcc tgccccgaaa
     4681 ccggcggaaa aaccggcaga gaagccgtct gaaaaacctg ccgaacattc cggtaatgct
     4741 tctgccaaag ccggcagcga gcagggtaat ggggaaggta agggaaccgg taccaaaggc
     4801 gacggaacgg ggcgcggaga aggcagcggt aaaggaagcg gcggtgccaa aggcgaacac
     4861 ggggaaggag ccggcggcag cggcggcgga accggtgtcg gcagcagcaa aggcaatcct
     4921 ttacgcgcca acggcagcat cccgcgcccg gcctatcccg cactttctat ggaaaatgac
     4981 gagcagggta tggttgtttt gagcgttttg gtttctccgg gcggtcatgt cgagtccgtc
     5041 aaagtggtga aaagcagcgg tttttcccgt ttggacaatg cggcacgcaa agcggcgcaa
     5101 aacgggcatt ttcaagccaa tgcctggacg gagtttaaag tccccgtcaa gtttgaattg
     5161 aattaggcgg ttgtcgcggc agtttgcggc agcttggatg tttgattggt tttttcagtg
     5221 ttggaatcat tatgaatttg aaattagtgt ttgaatcggg cgatcccgtc ctgatcggtg
     5281 tgtttgtgtt gatgctgttg atgagtaccg taacgtggtg tctggttgtc ttgcgctgca
     5341 tcaagctgta tcgggcgcgc aaagggaatg ccgccgtcaa acggcatatg cgcgatactt
     5401 tgtcgctgaa cgacgcggtc gaaaaagtgc gcgccgtcga tgcgcctttg tccaaactgg
     5461 cgcaagaggc attgcagtct taccgcaact accgccgaaa cgaagcgtcc gaactggcgc
     5521 aggctttgcc gttgaacgag tatttggtca ttcaaatccg caacagtatg gcgcagatta
     5581 tgcgccggtt tgattacggg atgaccgcgc ttgcctccat cggcgcgacc gcgccgttta
     5641 tcgggttgtt cggcacggtt tgggggattt accacgccct gatcaatatc gggcaaagcg
     5701 ggcagatgag tattgcggcg gttgccggcc cgattggcga ggcactggtg gcgacggcgg
     5761 cgggtttgtt cgtggcgatt ccggcggtgt tggcatacaa cttcctcaat cgcggcacaa
     5821 aaatactgac ccaggatttg gatgcgatgg cgcacgattt gcacgtccgc ctgcttaatc
     5881 aaaaggatag ctgattatgg catttggttc gatgaattcc agcgacgatt ctccgatgtc
     5941 cgacatcaac gttacgccgt tggtggacgt gatgctggtg ttgctgattg tgtttatgat
     6001 tactatgccg gtgctgacgc attccatccc tttggaactg ccgaccgcgt ccgagcagac
     6061 aaacaagcag gacaaacagc ctaaagaccc cctgcgcctg acgattgatg cgaacggcgg
     6121 ctattatgtc ggcggggatt ctgcaagcaa agtggaaatc ggggaagtgg aaagccgtct
     6181 gaaagccgcc aaggagcaga atgaaaacgt gattgtggcg attgcggcag acaaggcggt
     6241 ggaatacgat tatgtaaaca aagctttaga agccgcccgt caggcaggaa ttaccaaaat
     6301 cggttttgta accgaaacca aggcgcaata aagttcgacg cttgaacgaa tgccgcctga
     6361 aagtctttca gacggcattt cttatgttta atcagttata tatagttgat taaaacaaaa
     6421 atgagacgac agagcaccgt ctccatttct gcgcaggcgg gaatacaaac atctgatttt
     6481 tcttttattt tcaaacatta ctgaaatggg taagtctgga gtcctgcctg cgcgagaata
     6541 atgaaaatgt gcatttctaa tttttatagt ggattaacaa aaatcaggac aaggcaacga
     6601 agccgtagac agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga
     6661 atcgttctct ttgagttaag gcgaggcaac gccgtactgg tttttgttaa tccgctatac
     6721 ccgctgtaaa aacggagaag gacagaggac ggcagtaagg tttgccgtcc tctttttcaa
     6781 gttgcggtgg aatgccgctt gaaggtgtgg tttaaacgtt tcggcttgta atcagacgcg
     6841 ggcgttgtcc agccgctctc tgaagttttc cagattcaca cctgccgctt gggcgcggcg
     6901 gatgatttcg tcaaccggca taccttctgc cgcccggcgg aagccccaca ggagggagca
     6961 gcgcgtaccg gtccggcaat aggcaaggac gggatattcg gcttgtccga tgagttggcg
     7021 gaaggtttcg acatcgtgtt tttggatgtc gcgtgcggta acgggttggt gatggaatcc
     7081 ggtaacgcct gcttgttcca gccactgttt gatttgggcg aagtcgggtt gcgattcttc
     7141 ttcgcggtcg gggcggttgc agatgacggt tttgatgcca agttgcgcga tttgttccgc
     7201 atcggctttg gtcagttgcg gggagatgta gagatgttcg tcaagtttga gaatcgccat
     7261 tttgtttcct ttgctttgcg gttggtgttc agacggcatt ttgtgtgcaa tgccgtctga
     7321 aagtttaacc gaacaaccag ttttccaaca ggttgcgatc tgtgatgtgt aggatggcag
     7381 tatccgccgc cgttgcctga accgtcaaga cggtttgacg caatatgcct gcacggaaga
     7441 agtggatatt gatttttgcc ctgacgtggt atcgggccca ttgtgcggca aagtcggtgc
     7501 aggcataacc gtctaaagca atgattttgt cttgcgggca cagtgccgca gattccgcac
     7561 ttccgccgtt gaagacatgg gtcaggacga tatggtcggc gttttgtttg aagcgtgcgc
     7621 cgaaatcgcc cgtcggcggg atggggaaaa tgtgttccgc atatccgccg ccgtgttgtc
     7681 ggggaagcgg caggaaggtc agtttcacgc ctgtggttgc caggcattcg gcaagcggca
     7741 aatcttcggt gctgtacaat gccgtctgaa aaaaatctgt caaatccaag ccggtaattt
     7801 cctgacaacg gatttgccag tgtttttccg gaatacccga gtgtgtgtcc ctccactccc
     7861 gatagagttt gtccattacc gtatcaaggg aatgtctgcc gttgcttcgg ttgcgtatta
     7921 tcagatcaag gcacaatgcg gcaagtgcgc ctttttggta gtagctgacg atggcgttgg
     7981 ggctgttttc atccggtttg taaaatttgt tccacgcggt aaaactcgat tccgccaagg
     8041 tctgcctcaa acggccgcgg gtttgttgta cgcgcgtaat gccttgtgct agcaggttta
     8101 aataaaattc gggcgagatg gtgcggctgc gtgccaaaaa caaatcgtcg taataggatg
     8161 taatgccttc gaatgcccat agttgttcgg tatagttttc tttgtcgagg tcataagggg
     8221 cgaatgcggc aggtttgatg gatttgacgt tccacgcgtg aaaatattcg tgggagaaaa
     8281 gtccgagcaa tgtggtgtag gcatcgtcgg catcggtcat accgtacggc ggaaggctgt
     8341 ggcgatcggc gagcagtgcg gtgctgtcgg tgtgttccaa accgccgtaa atatggtcgc
     8401 cgacgtggag caggaacaaa tatttctcaa atggggcagg ggaggagaac atcgccagtt
     8461 ctgtttcgca gatttttttg atgtccgaaa ccagcctgtc gcggtcgaaa tcgggatata
     8521 tgccgtttaa ggcaatcgtg tgcggaatgc ctgccgcctc aaaatctaaa aattcaatca
     8581 agcccatctc gacaggtcgg tcaatcaatt cgtcataaga tgccgtctga aagacaagcc
     8641 tccctgtttc cggcagcgtt gtggcaatac gccattcggg tggaatgcct gtcaattcca
     8701 agcggtgcgg cagcgtttcc gttccttcga ctttcaaaaa caggcacgat ccgtcaaaaa
     8761 aaccgcgttc tgtcgtcagg aaagaacctc gaaccgacaa atcgaatgca tatacggtgt
     8821 agcggatttg ccactcgccg cgtacggcgg cagtgtgcca gcggtttttt gcaatttgtt
     8881 cgagcggcat ggacgtgccg ttgcaggatg catggataga agtgatgtgg cgggaaaaat
     8941 cccgaatcag atagctgccc ggcacccaat tcggaaggct tatttctata ggaaaatcat
     9001 tatcttgtgt gaatttcagc aggatgtgcc attcatggtc tagaggggac ggggctattt
     9061 tataatgaat cataactatc aaatatatcg ttaaacggga tggattaaat gcctttaaaa
     9121 tgcagcttaa cctacatcaa taaagtgcca caaaaaggga aaattggaaa caaaagcaat
     9181 ggcggccggg ttgtaattta ttataacaat atgatttata tgtaataaaa aacagattaa
     9241 gcaaagggtt cgccggcggc gcgaatgtaa ttccgcttgg cgtaataagg cagatatgtt
     9301 aaatttagaa aaaattacgc ttgcccggat tgcaccttaa acaggctgcg gaagcggcag
     9361 gataaaaatc tttattccca taattcatgg agactctcat ggacacacaa acttacaact
     9421 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggcatgttgg
     9481 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     9541 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     9601 gcggttgcgg tctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     9661 ttttcggcgg ttggctgccg gcatttacct tttggggttg gcaggcggta atcgttgccg
     9721 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     9781 cgattgacat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     9841 ttgccaaacg caaggtaaaa cacatttacg ttgccaactg gttctacggc ggctttattt
     9901 tggcggtcgc acttttgcac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     9961 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataacg
    10021 ccgtgggctt cttcctgact gccggctttt tgggtatgat gtactatttc gtacccaaac
    10081 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
    10141 tcacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
    10201 aatctttggg tatggttctg tccttgattt tgttcgcacc ctcttggggc ggtatgatta
    10261 acggcattat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
    10321 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
    10381 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
    10441 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
    10501 tgttcggcaa agagcagatg cacagcacca agctggtaga ggcgcatttc tggattgcga
    10561 ctatcggtgt ggttctgtat atcgccgcga tgtggattgc aggtgtgatg cagggtttga
    10621 tgtggagttc tctgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
    10681 gcaccatgcc ttactacatg atccgttttg caggtggttt attgtatctg agtggcatgt
    10741 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
    10801 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
    10861 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
    10921 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
    10981 gccggcgccg ggcgtgaagc cttacaatgc cctgcaggtt gccggacgcg atatttacat
    11041 ccgtgagggc tgttacaact gccactctca aatgattcgt ccgttccgtg cggaaaccga
    11101 gcgttacggt cattactctg ttgctggaga gtcggtttac gaccatccgt tccaatgggg
    11161 ttccaaacgt accggtcctg atttggcacg tgtgggcggc cgctattccg acgaatggca
    11221 ccgcatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
    11281 gtggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
    11341 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcgcctgagg ctttggcaaa
    11401 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
    11461 cgtaaggtaa catcatggat attaacggta ttcgcgctct tttcacggta tggatcttta
    11521 tctgtttcct gttagtactc tatatcgtct tcaacaggcg gaataagaaa aactacgatg
    11581 atgccgcaaa cagcattttt gctgaaaacc aagatgcgca agataagaaa agcgaaaacc
    11641 gttaatattg tgataacgga gcaaaacaat gaacgcaaca tcccaattta ccagtaattt
    11701 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
    11761 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
    11821 cgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
    11881 tgttttgacg tggctgttcg gtatcggtta tctggttatg tatccgggtg tcggcgacta
    11941 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
    12001 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
    12061 agacccacag gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
    12121 cggctctgat gccaaaggtt ctaaaggttt tccgaatctg accgatagcg attggttgtg
    12181 gggcggcgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
    12241 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
    12301 cctttcaaaa cccaaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
    12361 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
    12421 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
    12481 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
    12541 agacaaactg catatcatga ctgcttatgt atggggtctt tccaataaag acggtaaagc
    12601 tccggtgaaa aaagccgagc ctgcaccggc ggcagaaccc gcaccatctg ctcctgcaga
    12661 agcagcacaa gccgcttctg aagccaaacc tgccgcagca gaacctaaag ccgaggaaaa
    12721 agccgcacct gccgccaaag cggatggcaa acaggtttat gaaaccgttt gtgccgcctg
    12781 ccatggcaat gcaattccag gtattcccca tgtaggcacc aaagccgact gggccgaccg
    12841 catcaaaaaa ggtaaggaca cgttgcacaa acacgcgatc gaaggtttta atacgatgcc
    12901 cgcaaaagga ggtcgcggcg atttgagcga tgatgaggtc aaagctgcgg ttgactatat
    12961 ggtcaaccag tcaggcggta agttctaacc gttgttttcc gactttgtcg gatgcagaaa
    13021 tccggattca tatcgaatcc ggatttttta ttttgaacgg aaatcctgtt tttgatttat
    13081 gccgtctgaa agtggggatt ttgatgctta agcagtcaga ccgactgata tttttgtatg
    13141 gtttaaagaa tggtgcagtt gggtgtcgga cagcattgtt tgatgctttc agacggcata
    13201 taggtttgtt tcttcaaaat tgaatctttt ttcatatgtt gcaaaaaaat tgattttagt
    13261 cataaagaat tagtctttat tgtttataat cgcgcatcat cacgatagcc ttaatttcaa
    13321 aaggacgatt aatggataca caaatcaaaa ctgaagccgg tcagcagaat aaccggcatt
    13381 atctgagcgt ttggaggtgg catttttatg ccggtttatt ggttatgccc ttcttgaccc
    13441 tgcttgccgt tacaggtttg ggtatgctgt tgtttgccaa tattaccggt aaagagggag
    13501 agcgtattca cgttttccct caggcagtgg tgcaacccct ctctgttcag gcagaagcgg
    13561 cacgcggtgc cattaatccg gagacttcgt ccgtcgtcca atatattgca ccgcgtgccg
    13621 atgatatggt tgctgtgttc cgtgtcaaca atgagggtaa ggcaaccatg gttgcggtcg
    13681 atccttatac ggcaaaagtg gtcaacacca tgccgcgcaa tcagggttgg tatcacacta
    13741 tggatgaaat ccacggcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg
    13801 cagcttcact gaccattatt atggttgtca gcggcttgta cctttggtgg gcgaaacagc
    13861 gcggcattaa agcgatgctg ctgccgccaa aaggcagggc gcgttcttgg tggcggaatc
    13921 tgcacggcgc gtttggaact tgggtatcgt tgattttact gttgttctgc ctgtcgggta
    13981 ttgcttgggc aggtatttgg ggcggcaagt tcgtgcaggc ttggagtcag ttcccggccg
    14041 gcaaatgggg tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga
    14101 atgacggcaa ggttaaggaa gtgccgtgga ttttggagct tacgcctatg cctgtctcag
    14161 ggacgactgt gggtgaaaac ggcattaacc ccagcgagcc gatgacattg gaaaccgtcg
    14221 accgtttcgc gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg
    14281 aggacggggt atggactttg tcgcaggatt ctatgagtta tgacatgatc agcccgtttg
    14341 ccgaccgcac ggtgcatatc gaccagtaca gcggcaagat tcttgccgac atccgttttg
    14401 acgattacaa cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactt
    14461 tgggctggtg gagcgtgttg gcgaacgtcg tgttctgcct tgccgtgatt tttatcggca
    14521 tcagcggctg cgtgatgtgg tggaaacgcc gtccgtccgg cgtggcgggc attgttcctc
    14581 cggcgcaaaa aatcaaactg cccgtctggt gggcgatggc attgccgctg ctgttgattg
    14641 cactgctttt cccgaccgcg ctgcttgcca ttgccgtgat ttggctgttg gataccttgc
    14701 tgctgtcgcg gattcctgtg ttgaggaaat ggtttaaatg acccgatgcc gtctgaagcg
    14761 ttcagacggc attttgtttg cgggcggctt gcccgagaag ctatataatt ccgcatcact
    14821 gtcattttga aaaagcaagc catgttttac ctgtaccaat ccaaccgcct cgaatcgctc
    14881 gctgcactgt ttgcccgcat tcaaaaagtc aaaccgctga aatgtgcctt gcagcccgaa
    14941 cagattgttg tgcagagcca ggggatgcgc cgctacctca acacctgcct cgcccgcgat
    15001 ttgggcgtgg cggcgaattt ggcgttcagc ctgcccgccg gcctgacgtg gaagttgatg
    15061 aaaaaactga ttcccggtat tccggaactc agcccgtttg cacccgaagt catgcgctgg
    15121 cggctgctgg atttgttccg cagcgcagaa tttcaaaacg gagcggaatt tgaagatgtg
    15181 aggaatgtgc tgcaagacta tctgggcagc ggagaatcgg cggattacca gcttgcggga
    15241 cagcttgcag acatattcga ccaatacctc gtgtaccgtc ctcagtggat agacgcttgg
    15301 cagcagggca ggatactcgg tttgggcgac gacgaaatct ggcagtccaa actgtggcgt
    15361 tacctcgacg acggcaggca gagcgcgccg caccgtgtcg cgttgtggga aaaactgctt
    15421 gccgcgttgg acaaggataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg
    15481 gcgccgatgt atttgcagct tttgcacaag ctgtccgaac attgcgacgt gttcgtgttc
    15541 gcactcaatc cgagcgggat gcattggggc aacgtcatcg aagcggcgca aatcctcaaa
    15601 ggcggcggcg atcccgatct gacgcaggca gggcatccgc tgcttgcctc attgggcaag
    15661 caggggcgcg actttttcga ctttttgaac gaaatggaaa tagaaggcga aacgccggta
    15721 tttgaagaag gcggacgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg
    15781 aaaatgccgt ctgaaaatgt gggaagcgtc aacacgggcg acggctcgat acgcatcgtc
    15841 tccgcacaca gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg
    15901 catgaacatc ccgattggca gccgcatgat atcgccgtat taaccccgaa catcgaaccc
    15961 tatacgcctt ttatcgaagc cgtgttcgga caggcgcagc ccggcgcgca ggcattgccg
    16021 tattccgttt cggacgtgaa aatcagccgc cggcaaccgt ttttttatgc gttggagtgc
    16081 gtgtccgact tgctggaaag ccgttttgaa gtcggcaaag tgctcgcgct tttggaaacc
    16141 gcgcccgtgt tgcgccgttt cggactgacc ggggacgatt taccgctttt gcacgacatg
    16201 gttgccgatt tgaacgtccg ctggggtttg gacggagaaa tgcgcggcgg cacggatcag
    16261 ctgtttacct ggaagcaggc ggcagaccgt atgatattgg gctggatgct gcccgaaggc
    16321 ggcaacccga tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg
    16381 ttcggacgtt ttgccgcctt cctcgaaacc ctgtcggata ttgcccggat atggcggcaa
    16441 cctgcgacgg tcggcgaatg ggtggcgcgt tgccgggatt tgcttgaaac attgtttcaa
    16501 gccgggcccg atgaccaaaa ggcagtccaa aacctcgaaa acgaatgggt caaatggcag
    16561 gaagagagca ctttggctgg gtttttcgga cagttgccgc aacaaaccgt catccgccat
    16621 atccgccgct tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt
    16681 tgcagtatgg tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac
    16741 gacggagatt ttccccgcaa taccaaagcc gccgtattcg acctggttgc caaacatccc
    16801 gccaaaggcg accgcgcccg ccgcgacgac gaccgctacc tgttcctcga agccctcatc
    16861 agcgcgcgcg aaatcctgta tttgtcctac atcgggcgcg acatccgcaa agacgaagag
    16921 cttgccccgt cttccctgtt gggcgaactc atcgacacag ttgccgccat gaccggcaca
    16981 aacagccgcc aacttgcaca aaattggata gaacagcatc cgctgcaagc cttctcgcgc
    17041 cgatatttcc aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc
    17101 gccgcgctcg gacaaacgcc cgaaccgccg cgcccttttt tcgaccaacc cgtagaaaac
    17161 gccgaacccg ttgccgaaat cggacaggac gaatttatcc gcttctggcg caaccccgtc
    17221 aaagtatggc ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag
    17281 cctgccgaac ccttcgagcc gcaacacgcg gagcaaatcg ccgaaaccta tatcggggcg
    17341 cgccgcgaag gacaagattt ttcccaaacc gccgcccgta tcgcggcgga aagcctgctg
    17401 ccttccggag aattgggaaa actttggcag cagtccttcc aaaccgccgc caaacaaatc
    17461 gacacggcgg ttttaaacag ccccaaactg cccccgtttc catacgccat accgtcggac
    17521 gggcaaatcc tgaaaggcag cttgggcaat ctgtaccgct gcggtcaggt gttttatgcc
    17581 tacggcaaac ccaatgcgcc gcaacgtatc gcttttttgt tggaacacct gatattttgc
    17641 gccgttatgc cgtctgaagc cgaaacgcgg caaaccttta tcgtcaaatc cggagaaacc
    17701 gaagtattgg cggaaatcgc gcaagacagg gcattgcagc tgttgtcgga atggattgta
    17761 tttttcaata tcgggcaaaa ccgcccgctg ccgttttttg ccaaaacctc gcttgccgcc
    17821 gccgaagcgt ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc
    17881 tattacggca gcaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc
    17941 ggcaacgcaa gccaaaaccc gctcgaacag cccctgtttg aaaacctcgc ccgcctgctc
    18001 gccgacacac ttgccgcagc ggaaaagagg gaagggaccg gagaagcctg acgggatgga
    18061 aaggcaaata ccgtctgaaa acagaaaaaa ggttttcaga cggtattttg tttatcgtgt
    18121 ttaaggcacc ggatagaatg tgggtgcgcc gggaccgacg ggcaggccca aaacaaatac
    18181 ccaaatgcag aataaggcga tccatgcaat taagaagaaa gcggaatacg gcaacatcat
    18241 agaaatcagc gtgcctacgc ccgcatcttt tttgtatttg attaccgtcg ccataatcag
    18301 cccgaaataa ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc
    18361 ggcttgaatg acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc
    18421 agttaccgcc cattgcgcgg aagcggagcc tatcatcaga ttgataaaag cacaaattaa
    18481 aataaaaccg ataaacaaca cactgccgcc caagccgact tcttttaaga acaccgcccc
    18541 tttaacggca atatattgcc caatattcgt ccaattaaaa aatgcgacaa actgtgcggc
    18601 aaaaaagatg atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac
    18661 gacttcccgt tcgccgcgca aacttcgggt tatccggcca taaacaatgc ccggcagcgc
    18721 aaacaacaag aaaataaaaa caacaatcga ttttaaaaac ggcgaaccgg caaccaatcc
    18781 tgtttcagga tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga
    18841 taaggcaaca aacaccacgc ctgcccaaat taatccttta tattccaaag gcgtgatttc
    18901 attggaatgc cgaatgtctt tttcttcttg tgacaaatct gactgataag ggcccaattg
    18961 cggttcgacg attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggt
    19021 taccataaaa aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc
    19081 cgcctgttgg gtaatgcctg ccaagagcgg atcgagtgtg cctaagaaca gattggccga
    19141 ataaccgccg gaaacgccgg cgaaagccgc agccagaccg gcgagaggat ggcggccgag
    19201 cgaatgaaag atgacggcgg acaaagggat taggacgaca tagcccaatt cagaagccgt
    19261 attggataaa atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gggattttgt
    19321 gagcaataag cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa
    19381 taaagaaacc aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt
    19441 caggattttg atcaaaccgt cggcatcgag caggctgaca acgtgaatca aaccgtcatc
    19501 ggcacgtcct ttcgccccaa caggacgcgg atcggggacg gatagtccga aatacgcacc
    19561 gacggcagag gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg
    19621 caacatattg cccagccatt cgactgtgcg taaaaatcgt ccgctacgac gcgcgtcggt
    19681 ttgactcatc tgcttctcct taaaaaaata tattgagtgc attaaatttt tgaatttaac
    19741 atgaagtgtt ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa
    19801 aacggaaaat gcccacatcg ttattctcat aaaggcggta atccgaaaac ttgacgttaa
    19861 agccttatca ggaatgactg gaactcaaaa aaacgaattc ccgcctgcgc gggaatgacg
    19921 ggattttcgg tttttgtttg caagaatgcc ggagaaaaat gcagtttaca ttcgaatggt
    19981 attgttatta attgtaaata aatattggaa ggtaaatgat atgaaaaatg aaattcaaaa
    20041 aatcatggat aaatatgatc catggcatga agatgatttt gagtcgtatg aagacattgc
    20101 caaagatgta tcgttgatga cagataaaac gttcattgaa cattatttgt tagaagttta
    20161 ttcagaagaa aacggacatt ttgaccaaga gaatatccat gcgatgatag gggaaattaa
    20221 aaatgcaatt tagtgaatat gtatttttag aagaatgctt tgtgtcattt tgatgccgtt
    20281 tgaaagggtt tgttcgtttc agacggcatt gttaccaagg cttgattatt tccggcgcag
    20341 gcagggatgg ttttccaagt tgtccatcat tatcccgatg atgcgcgggg cggttttgcc
    20401 caaatcgaaa ctttcgccgg aagagaacca acgccaaatc agcccgtcca acgtcgattt
    20461 gatgaagatg accgccgttt ccttgtccaa atcgtcagcc aaatcctgat tttccaccgc
    20521 ttcggtcaaa acggcggtaa ttttctcgcg ccagattgcc tgatgcttgc gggcaatggc
    20581 gataacggcg gcgttttgct ccgtgtgttc gcattttaaa aacaggatgt tgtggaattt
    20641 gtagtagatg tcgttgcttt gcagccgctc gaaaaagtgc agcagcgtgt ggcggaatac
    20701 cgtccaagaa cctccttcgg catctgcggc atcttgcgcg atgcagtttt cgatgtcgtc
    20761 gcagatacgt tggaacaacg cgtcaaacaa gtcttcctta tttttgaaat gccaatagag
    20821 cgcgccgcgc gttacgccgg cggcttgggc gatttcgttg agcgaggtgc gggcaatccc
    20881 tttgcggtaa aaggtttcca aggcggcaag catcaggtgt tctttggttt tcaaggcttc
    20941 ggttttggtt tttctcataa tggcgttttc gtttcgggtc ggtttgacga gggcggatta
    21001 taaaaaagac ttttatccgt gcaatcgtgt atgtataatg aaacccataa aattgagact
    21061 gcatctcaac tttgaaaaca ggtataacct gctttgcaac ccgttcgaac attcgggtat
    21121 caaagcaggc ggtttttata tcctgaaata agaatgcctg ttgattggca aaacccttta
    21181 catcaaacca gacagatagg ataaataatg gctttttatg cttctaaggc gatgcgtgcg
    21241 gccgcgttgg ctgccgccgt tgcattggca ctgtcgtctt gcggtaaagg cagagacgcg
    21301 gcgcagggcg ggcagcctgc gggtcgggaa gcccccgcgc ccgtcgtcgg cgtcgtaacc
    21361 gtccatccgc aaaccgtcgc attgaccgtc gagttgccgg ggcgtttgga atcgctgcgt
    21421 accgccgatg tccgcgccca agtcggcggc atcatccaaa aacgcctgtt ccaagaaggc
    21481 agttatgtcc gcgccgggca gccgctgtat cagatcgaca gttccactta tgaagcaggt
    21541 ctggaaagcg cgcgcgcgca actggcaacg gcacaggcaa cgcttgccaa agcggatgcg
    21601 gatttggcgc gttacaaacc gctggtttcc gccgatgcca tcagtaaaca agagtacgat
    21661 gctgcggtaa cggcgaaacg ttctgccgag gcgggcgtta aagcggcgca ggcggcgatc
    21721 aaatccgccg gcatcaatct gaaccgttcg cgcattaccg cgccgatttc gggctttatc
    21781 ggtcagtcca aagtttccga aggtacgctg ttgaatgcgg gcgatacaac tgttttagcc
    21841 accatccgcc aaaccaatcc gatgtatgtg aacgttaccc agtctgcatc cgaagtgatg
    21901 aaactgcgcc ggcagatagc cgaaggcaag ctgctggcgg cggatggtgc gattgcggtc
    21961 ggcatcaaat ttgacgacgg tacggtttat cctgaaaaag gccgcctgct gtttgctgat
    22021 ccgaccgttg acgaatcgac cggtcagatt accttgcgcg ccgccgtatc gaacgatcag
    22081 aatatcctga tgcccggcct gtatgtgcgt gtgctgatgg atcaggtggc ggcggataat
    22141 gcattcatcg ttccacagca ggcggtaacg cgcggtgcga aagataccgt gatgattgtg
    22201 aatgcccagg gcggtatgga accccgcgag gtaacggtcg cgcaacagca gggtacgaat
    22261 tggattgtta cgtcgggtct gaaggacggg gacaaggtgg ttgtggaagg catcagtatc
    22321 gccggtatga cgggggcgaa aaaggtaacg cctaaagaat gggcgccgtc tgaaaatcaa
    22381 gctgccgccc ctcaagccgg cgttcagacg gcatctgaag ccaaacctgc ttctgaagcg
    22441 aaataaggaa ggcatcgatg gctaaattct ttatcgaccg ccccattttc gcgtgggtta
    22501 tttcgatttt cattattgcg gcaggtattt tcggcattaa aagcctgccg gtttcgcaat
    22561 atccgtccgt tgccgccccg accattactt tgcacgccat ttatccgggt gcgtccgcac
    22621 aagtgatgga aggcagcgta ctttccgtga tcgaacggaa tatgaacggc gtggaaggtt
    22681 tggactatat gtccacttcc gccgattcga gcggcagcgg cagcgtgagc ctgaccttta
    22741 cgcccgatac cgacgagaat ctggcgcagg tggaagtgca gaacaagctt tccgaagtat
    22801 tgagcacgct gccggcaacc gtccagcaat acggcgtaac cgtatccaag gcgcgttcca
    22861 atttcctgat gattgtgatg ctttcgtcgg atgtgcagtc aaccgaagag atgaacgact
    22921 acgcgcagcg caacgtcgtt cccgaattgc agcgtatcga aggcgtgggg caggtccgcc
    22981 tgttcggcgc gcaacgcgcg atgcggattt gggtcgatcc taagaaactg caaaactaca
    23041 atttgtcgtt tgccgatgtc ggcagcgcgt tgtcggcgca gaatatccaa atttccgcag
    23101 gttccatcgg ttcgcttccc gctgtgcgcg gacagacggt tacggctacc gtaacggcgc
    23161 aagggcagtt gggtacggca gaagaattcg gcaacgtcat cctccgcgcc aataccgacg
    23221 gttctaatat ttacctgaag gatgtggcga aagtcggatt gggtatggaa gactattctt
    23281 cctcaacccg cctgaacggg gtaaatacca ccggtatggc ggtaatgttg tccaacagcg
    23341 gcaatgcgat ggcgacggca aaggcggtaa aagaacgctt ggcggtgctg gaaaaatact
    23401 ttccgcaggg tatgagttgg aaaacccctt acgatacttc caaattcgtt gaaatttcga
    23461 ttgaaaaagt gattcacact ttaatcgaag cgatggtgct ggtgtttgtc gtaatgtacc
    23521 tcttcctgca aaacatccgt tatacgctga ttccgaccat cgtcgtaccg atttccctgt
    23581 tgggcggttt cgccttcatc tcttatatgg ggatgtcgat taacgtattg acgatgtttg
    23641 cgatgacatt ggtcatcggc atcgtggtcg atgacgcgat tgtggtggtt gaaaacgtcg
    23701 agcgcattat ggcgggtgaa ggcctgccgc ccaaagaagc gaccaaaaaa gcgatgggtc
    23761 agatttcggg cgcggtcatc ggtattaccg ccgttctgat ttccgtgttc gtgcctctgg
    23821 cgatgttcag cggggcggcc ggcaatattt acaaacagtt tgccctgacg atggcgtcat
    23881 caatcgcatt ctccgccttc cttgcgctga cccttacccc tgctttgtgt gccacaatgt
    23941 tgaagacaat cccgaaaggg catcacgaag agaaaaaagg tttcttcggc tggttcaaca
    24001 agaaattcga cagttggacg cacggctacg aaggccgggt tgccaaagtg ctgcgtaaga
    24061 ctttccgcat gatggttgtc tatatcggct tggcggttgt gggcgtgttc ctgtttatgc
    24121 gcctgccgac ttcgttcctg ccgaccgaag accaaggctt cgttatggtc agcgtgcaac
    24181 tgcctgcggg tgcgaccaaa gagcgcaccg atgcgacgct agcacaagtt acccaactgg
    24241 cgaaaagcat tcccgaaatt gaaaacatca ttaccgtttc cggcttcagc ttttcgggca
    24301 gcggtcagaa tatggcgatg ggttttgcca tattgaaaga ttggaacgaa cgtaccgcct
    24361 ccggcagcga tgccgttgcg gttgccggca agctgacggg tatgatgatg gggacgctta
    24421 aagacggttt cggcatcgcc gtcgtcccgc ctccgattct ggagttgggc aacggttcgg
    24481 gcctgagcat caacctgcaa gaccgcaaca ataccggcca taccgcattg ctggcgaagc
    24541 gcaacgagct gattcagaaa atgcgtgcca gcggtttgtt tgaccccagc accgtccgtg
    24601 ccggcggttt ggaagactcg ccgcagttga aaatcgacat caaccgtgcc gcagcggcgg
    24661 cgcaaggcat ttcgtttgcc gacatccgca ccgcattggc aagcgcgctg agttcgtctt
    24721 atgtcagcga cttcccgaac caaggccgtc tgcaacgcgt gatggtgcag gccgacgagg
    24781 atgcccgtat gcagcctgcc gatattttga acctgaccgt gccgaacaaa tccggcgtcg
    24841 ccgtaccgct ttccaccatt gctactgttt cttgggaaaa cggtacggaa cagagcgtac
    24901 gcttcaacgg ttatccctcg atgaagctgt ccgcttcgcc tgcaaccggc gtttccaccg
    24961 ggcaggctat ggcggcggtt caaaaaatgg ttgacgaatt gggcggcggt tacagcttcg
    25021 aatggggcgg acagtcgcgc gaagaggcaa aaggcggctc gcaaaccctg attttgtacg
    25081 gtttggcggt tgcagccgta tttttggtac ttgccgcgct ttatgaaagc tggtcgattc
    25141 cgctggcggt catccttgtg attccgttgg gtttgatcgg tgcggctgcg ggcgtaaccg
    25201 ggcgcaacct gtttgaagga ctgttgggca gcgttccctc gtttgccaac gacatctact
    25261 tccaagtcgg tttcgttact gtgatgggtt tgagtgcgaa aaacgcgatt ctgattatcg
    25321 aatttgccaa agaccttcaa gcgcaaggga aaagcgcggt cgaagccgct ttggaggcag
    25381 cccgcctgcg tttccgtccg attatcatga cctcgtttgc ctttattttg ggcgtggttc
    25441 ccctgtatat tgccgctggt gcgagttctg ccagccagcg cgccatcggc acgaccgtat
    25501 tctgggggat gctcatcggc acgctcttgt ccgtgttcct tgttccgctt ttctatgtgg
    25561 tggtgcgcaa attcttcaaa gaaactgcac acgagcatga aatggcggtc agacatgcat
    25621 caaaagcggg tataaccggt tcggacgata aacaatatta agcaaaatac cgtctgagaa
    25681 ccgccggttt tcagacggca tcaggacttt tttatgaata ctacattgaa aactaccttg
    25741 acctctgttg cagcagcctt tgcattgtct gcctgcacca tgattcctca atacgagcag
    25801 cccaaagtcg aagttgcgga aaccttccaa aacgacacat cggtttcttc catccgcgcg
    25861 gttgatttgg gttggcatga ctattttgcc gacccgcgcc tgcaaaagct gatcgacatc
    25921 gcactcgagc gcaataccag tttgcgtaca gccgtattga acagcgaaat ctaccgcaaa
    25981 caatacatga tcgagcgcaa caacctcctg cccacgcttg ccgccaatgc gaacggctcg
    26041 cgccaaggca gcttgagcgg cggcaatgtc agcagcagct acaatgtcgg actgggtgcg
    26101 gcatcttacg aactcgatct gttcgggcgc gtgcgcagca gcagcgaagc agcactgcaa
    26161 ggctattttg ccagcgttgc caaccgcgat gcggcacatt tgagcctgat tgccaccgtt
    26221 gccaaagcct atttcaacga gcgttatgcc gaagaagcga tgtctttggc gcagcgtgtc
    26281 ttgaaaacgc gcgaggaaac ctacaagctg tccgaattgc ggtacaaggc aggcgtgatt
    26341 tccgccgtcg ccctgcgcca gcaggaagcc ttgattgaat ctgccaaagc cgattatgcc
    26401 catgccgcgc gcagccgcga acaggcgcgc aatgccttgg caaccttgat taaccgtccg
    26461 atacccgaag acctgcccgc cggtttgccg ttggacaagc agttttttgt tgaaaaactg
    26521 cctgccggtt tgagttccga agtattgctc gaccgtcccg acatccgcgc cgccgaacac
    26581 gcgctcaaac aggcaaacgc caatatcggt gcggcgcgcg ccgccttttt cccgtccatc
    26641 cgcctgaccg gaagcgtcgg tacgggttct gtcgaattgg gcgggctgtt caaaagcggc
    26701 acgggcgttt gggcgttcgc gccgtctatt accctgccga tttttacttg gggaacgaac
    26761 aaggcgaacc ttgatgtggc aaaactgcgc caacaggcac aaattgttgc ctatgaatcc
    26821 gccgtccaat ccgcctttca agacgtggca aacgcattgg cggcgcgcga gcagctggat
    26881 aaagcctatg acgctttaag caaacaaagc cgcgcctcta aagaagcgtt gcgcttggtc
    26941 ggactgcgtt acaaacacgg cgtatccggc gcgctcgatt tgctcgatgc ggaacgcatc
    27001 agctattcgg cggaaggtgc ggctttgtcg gcacaactga cccgcgccga aaaccttgcc
    27061 gatttgtaca aggcgctcgg cggcggattg aaacgggata cccaaaccgg caaataaccg
    27121 cccgggcaaa caaatgccgt ctgaaacgat aaggtttcag acggcatttc tttctttata
    27181 gtggattaac aaaaaccagt acggcgttgc cccgccttag ctcaaagaga acgattccct
    27241 aaggcgccca agcaccgggc gaaccggttc cgtactattt gtactgtctg cggcccgccg
    27301 ccttgtcctg atttttgtta atccactata tgtttgacgg ttgcggtcag gctgcgcggc
    27361 gtgccgtaaa cgtgaatgtc gggcatcgtg cggcagcgtt tgttaaagac gttttcaaag
    27421 tccaaaccga tgcgggtgga tttgccgatt ttgtaatgcg cggtcaagtc cagtgtagcg
    27481 tatggggcgt tgggtcaatc cggctgcggc gacagggaag aaaccgccat acccgtcggc
    27541 atcccgaagc cgaatacggc aacggcaagc gcaatcagca gcgtataaag ccccgcctga
    27601 tagcctttca gcttcaggac ggtcagcgcg gcaaagaaaa agacgatggg taagagtgcg
    27661 gcggcggcag tcagatacag gctgccgccg attgccgtgt agttttgaac ccaagtttcc
    27721 ataattgaac atctccgaaa atatttttct aatcgtcggc aatagtggtc aaaccaatta
    27781 aagcaacgtt gcattacttt acgaaacttt aatattcagg tcaatatatt tttgggcggt
    27841 tcggcagatt tgaatcggag ctttcattta aatcccgtta aaacaaatat ttgcatgaac
    27901 aaaaattgta gtttggtgta gttttttcct gtgtttcggg ggcggtgcga ggtaagtgcc
    27961 gttgacggcg ggaacggcgt gtcattagaa tccgccctga ttggccagtc caatttgatg
    28021 tttgatgtat ttcggaaggg ttctgcaggg aatctttata aaggaggagt gatgaaactg
    28081 gtaaggcctc agaaaatcag cgatcaggta ttgtcggtat tggaaggccg catcgtggaa
    28141 ggggtttacg cggaaggggg caagattccg cccgaacgcg ttttggcgga agagttcggc
    28201 gtttcgcgtc cgtcggtccg gtcggcattg aatattttgg ttgcccgtca gattttggaa
    28261 gcgaggcagg gcgatggtta ttatgtttcg gtcaagccgc agcaggattt tttgcaaagt
    28321 tggcaggagc ttttgggcaa acattccaat tgggaacagg atgtttttga tttcagctgc
    28381 cacatcgagg gctgtatggc ggcattggcg gcagaacgcc ggacggatgc cgatttgaag
    28441 cggattcggt tttggcttga aaagtttgag gaagcgtgcg gaagcggcaa tctggaacat
    28501 cagggcgaag ccgatgtcag cttccatcag acgattgcgg atgcggcgca caatttgttg
    28561 ttcagccatt tgtcgggcgg tttgttgaaa atgctgtacc ggcagacgcg cagcagcctt
    28621 atttatctga atcaggaaga agatccgcgt ccgaaactga tggcgcagca ccgcgtgctg
    28681 tatgaggcga tatcgaaccg ccggccgggc gaggcttcgg aggcggcaaa ggctcatttg
    28741 aattatgtgg cgagcagcat attgaaagac agggaatatc agagccgtaa ccgccacgcg
    28801 gatactttgg cgcaaaacga tttgaagcgc gtgcaggatt gggaggtatg aataccgttc
    28861 agacggtctg gcatattcgg atgtatcggt atagtgaact aaatttaaac cagtacggcg
    28921 ttgcctcgcc ttgccgtact atttgtactg tctgcggctc gccgccttgt cctgatttaa
    28981 atttaattca ctatagtatt acccgattcg aacgggcgga taatgaaatg ccgtctgaac
    29041 agcatttgcg gttcggacgg cattttgttt gcggggcgga ggcgtttgct tagaagcctt
    29101 gcgccagcat cgcatcggca actttgacga agccggcgat gttcgcaccg ttgacgtagt
    29161 tcactttgtc gccgactttg ccgtatttca ggcaggactc gtggatgctt tgcatgatgc
    29221 cgaacagtcg gctgtccact tcttcgcgcg tccaagacag gcggatggcg ttttggctca
    29281 tttccaagcc cgaagttgcc acgccgccgg cgttggaggc tttgcccgga gcgtagagga
    29341 tgccggcttt gataaattgc tcgaccgcgc ccaaagtcga cggcatattc gcgccttcgg
    29401 caacgacgta gcagccgttt gccaacaggg ttttggcggc ttcttcgtcc aattcgttct
    29461 gggtcgcgca gggcagggcg atttcggcgg caacgcccca cggtttttgg ttttcaaagt
    29521 attgcaaacc ttgctctttg gcgtaggtgg caacgcgttc gcggcggact tctttcaatt
    29581 cgatcaaggc ggcgagttgc gcttcggtca taccgatgtc ggggaagagg acgaagccgt
    29641 cggagtcgga gacggtcagc actttcgcac ccagttggat ggctttttca gcggcgtatt
    29701 gcgccacgtt gccggaaccg gaaatcagaa cgcgtttgcc ttcaaaacta tcgttgcggg
    29761 tttgcagcat cgcttgggcg aaatagacgc agccgtagcc ggtcgcctcg gggcggatga
    29821 ggctgccacc ccattccaaa cctttgccgg tcaggacgga agtgaattca ttgcggattt
    29881 ttttgtattg tccgaacagg tagccgattt cgcgtccgcc tacgccgatg tcgccggccg
    29941 gaacatcggt gtccgcgccg atgtggcggt agagttcggt cataaaggct tggcagaagc
    30001 gcattacttc ggcatcggat ttgcctttgg ggtcgaagtc ggaaccgcct ttgccgccgc
    30061 ccataggcag ggtggtcaag gcgtttttga acacttgttc aaaagcgagg aatttcaaca
    30121 cgcccaaatc gacggtcggg tggaagcgca ggccgccttt gtaagggccg atggcggaac
    30181 tcatttgcac gcggtagccg cggttgactt ggacttgtcc tttgtcgtcc tgccaggtta
    30241 cgcggaacat cacgacgcgt tcgggttcga cgatgcgttc cagcaggctt tgctgggtgt
    30301 atttcggatt ttttgccaaa aacggatcaa ggctcatgaa gacttcttca accgcctgat
    30361 ggaacggctc ctgattgggg ttgcgttgtt tgaggttggc aaacagggtg ttcaggtcag
    30421 tcattcggat actccttttg gttgatattg tactaatggg aaaacaaatt tgtagttggc
    30481 aatataaacc tatttttttc tgatgccaag cctaattttg cacaaattgt tgacattatt
    30541 tttcccatat tttcgatata tgatgtttat tgccgataat caaccaataa tcataaatta
    30601 atttctcaaa taaaaaatat tattggaatt ttattctaag tagttaaact agattttttg
    30661 attccaatca agataatttt gttttcacgc ctgcttttgc tgtgaaaaag ggctgaaatt
    30721 gccaacaatc ctgcctgcct tcatccgcgt tttgagcgtc cggcggggcg gcctgcgcgt
    30781 ttgtccggac tcgggtgttg gccggaaatg ttacaatatg tgccgtttaa tttgatgaaa
    30841 gatgatgatg aaggcctatt tggacttgat gcgccacgtc ctcgacaacg gtacggacaa
    30901 atcagaccgc accggtacgg gtacgcgctc cgtattcggt tatcagatgc gttttgactt
    30961 gggcaaaggt tttccgctgc tgacgaccaa aaagctgcat ttgcgctcga taatccacga
    31021 gctgctttgg ttcctcaaag gcgataccaa tatcaaatac ctgaaggata acaatgtttc
    31081 catttgggac gagtgggcgg acgaaaacgg cgacttgggc ccggtttacg gctaccaatg
    31141 gcgcagctgg cccgcgcccg acggcaggca tatcgaccaa atcgccaatg tggtggaaca
    31201 aattaagaaa aaccccgact cgcgccgcct gattgtgtcg gcgtggaatc cggctttggt
    31261 cgatgagatg gccttgccgc cctgccacgc gctgtttcag ttttacgttg ccgacggcaa
    31321 actgtcctgc cagctttacc agcgtagtgc agatattttc cttggtgtgc cgtttaatat
    31381 tgccagctac gcgctgttga cgatgatgat ggcgcaagtg tgcgggctgg aggcgggcga
    31441 gtttgtccat acgtttggcg acgcgcattt gtaccgtaac catttcgaac aggccgcatt
    31501 gcagttggaa cgcgaaccgc gcgccttgcc tgtgatgaaa atcaatcctg aagtcaagga
    31561 tttgtttgcc ttcaagtttg aagatttcga gttggaaggc tacgatccgc atccgcacat
    31621 caaggcggta gtgtcggttt aggaggcggt atgggcaggc atttcgggcg cagacgtttt
    31681 ctgacggctg ccgccgttgc tgtggccggt gcggcggttt cttttttgcc gaatcctttt
    31741 gccgccggcg gcgaaaaacg caacatggat aaaaaacgcg atgaaaatgt gtttttctgg
    31801 aaaggtgtcg cgctgggttc cggcgcggag ctgcgcctgt tcggcgtgga cgacagacag
    31861 gcggcggatt tggtcaataa ggttttggcg gaagtggcgc gtttggaaaa aatgttcagc
    31921 ctttaccgtg aagacagcct gatcagccgt ctgaaccgcg acggttatct gacttcgcct
    31981 ccggcggatt ttttggaact gttgagcctg gccgcgatat tcacgcgctg acggaaggcg
    32041 cgttcgatcc gacggttcag gcgttgtgga aactgtatgc cgattatttt gccgcccgtc
    32101 ccgatgcgga aacgccgccg gagaatagcg tgaaggaaac cctgaaacgg gtcggcttcg
    32161 gcaaagtctc gtttgacgac cgtgaaatcc gttttgccga aaagggtatg ggtttgtcct
    32221 tgaacggcat cgcgcaaggc tatatcacgg acaaggttgt tgcgctgctt aaagcaaacg
    32281 gagtgcctgc cgcgctggtc gatatgggcg aaatcagggg gtttgataca aacggcaggc
    32341 ggatgtggaa tgtcggcatc cgcaatccgg acgacgaaga aggcgtgttg gcaaatatca
    32401 cgatgaaaga caaggcgttt gccacttcgg gcggctatgg gacggtgatg gacaaagcgg
    32461 gcaggcttac ccatttgttc gatccgcgaa ccggcgtgag tacgccgcgt tataaaagca
    32521 tgagcgtaat ggcggatgat gcggcggttg ccgatgcctt gtcgacggcg ttttccgtga
    32581 tggatttgcc gctgatacgg tcggttgccg aaagcaggcg gcttaaagtt aggctggcca
    32641 tgccggacaa tatagtggat taacaaaaac cggtacggcg ttgcctcgcc ttgccgtact
    32701 atctgtactg tctgcgactt cgtcgccttg tcctgattta aatttaatcc actatacttt
    32761 gaacgaaata ggttgaatct gaagcaaaat gccgtctgaa aggctttcag acggcatttt
    32821 gccgtcgggc ggtatgttta aaggatgcct aaagaattga cgaaaacaat aataatggca
    32881 atcggggcaa ggtagcgcaa ggtattcagc cacagcagca gcactgcccg tggtacggtg
    32941 ctgcccgcgc cggcatcttt taacacagac tgcttgtcct gaatccaggc ggtaaagatg
    33001 gaaacactca aagcaccaat cggcataatg acggcggaaa taacatagtc ccacaaatca
    33061 aaaatggttt tgccgaaaac cttaaactca ccccatgcgc cgaaagacag cgcggacggg
    33121 atgccgacaa tgaaaatagc cgtgccgata agccaagtat gttttttgcg tttgcgctcg
    33181 tcttggcgga tggttgaggc aatgaccgtt tccaacatcg aaaatgccga agtcagcgtg
    33241 gcgaaaacga ccagaagcat gaatactgca aataaaaccg taccgaacgg cattttcatg
    33301 aacactgcgg gcaatacgat aaaaatcaat cccggcccct ggttcggttc aaaaccgaag
    33361 gcgaataccg ccgggaaaat caccaggccg gcaagcaggg aaaccaagag gttcatccac
    33421 ataatcgtat ggccggaacg gaacatatcc tgatcttttc ccaaatacga agcataggta
    33481 atcatggcgg aaacgccgat gctcagggca aaaaacgcct ggcccaaagc cgtaatcatc
    33541 gtatcggctt taaagtacga ccaattgggt ttgagcagga aagacacgcc ctccattgca
    33601 tccggcagcg tcagcgaacg gacagccagc gcaataaaga ggataaacag ccccggcatc
    33661 agataacggt ttgccttttc aatgccgtct gaaatgccgc ctttgaccac ccaaaccgta
    33721 atcagcataa acagtgcctg ataggacagc gaacctgccg gattggaaat cgttgtgccg
    33781 aacagggctt caaagtccgc tcccgcatgg attgcccctg taaaactgtg gacgacataa
    33841 ttcaataccc atccgccgac cacgctgtaa aacgacagca aaataaagca ggcggcaacg
    33901 cccatgcgcc cgacccaaag ccattgcgta cccggacgca gaaccctgaa ggaatcgacg
    33961 gcatttttac cgcccgtgcg cccgatataa aattcggcaa gctggacggg cagggcgacc
    34021 aagacggtaa atatcaaaaa cagcaggaaa aacaccgcgc cgccgttggt gcccgccgta
    34081 taaggaaatt tccaaatcgc gcccaaaccg atggccgaac ctgccgcagc aagtacgaaa
    34141 ccgattttgg acgaccaaga agtatggtta ctcataaaat ttattaagaa atgtgaaggt
    34201 agtgcattgt aacaaagcac ttgacgcggc ggcacaagcc ggggaagacg ctgcaaaact
    34261 ttgccaatag gctgcgcgat atcgggggat attgggattt tcttattttg aaggatgaat
    34321 tcggggtgtt tttttccaat gaacgaatat aatttggcgg tttgttgcat caaaggcttt
    34381 cagacggcat aggccgtctg aaagcggctt atattgcatc caataatttg tcggcggtat
    34441 ggttgatggt gaaacgggcg gcgacgcatt gatgtcctgc cgttgccagt gcgttttttg
    34501 cttcggggtt taagacgagg cgttcgattt cgtccgccaa agattcaggc gtaagcctgt
    34561 ccagcagaat ccccgattga tgatgttcga taatttcctt ttgcgcaccc aaagtattgg
    34621 aaatcaccgc cgttcggcag tacatcgcct cgcataatga caaaccgaat gcctccggga
    34681 cgaggctggg caaaaccacg acatcgcttt ggcggtaaaa cgaagcaagt ttttcggtaa
    34741 acccttcaaa agaaacaaat ggttctgctc ccgaagcaga tacgccccgc ttcaggcggc
    34801 acatataatc cggatgtccg tgccctgcca atttgagcct gatttgagga tatttccgat
    34861 gcagtatcac acaggcttca atcaggtttt ccaatccttt ttccggactg atcctgccgg
    34921 cgtaggcgac ggtaaaaaaa cggctgtcgg gtttttcttg agacgggggg aaccggccgg
    34981 tatcgatacc gttatgaaca atccggtatt tttctttaaa gggattgtcg gcggtttgca
    35041 catcgtaaac cagacgggaa acgcaaataa agcggtcggt gtttttctgt atcaggcggt
    35101 gataaaaatc ggttttgttg gcgacgacat tatgtttgac aaatatcagg cgcaccccgg
    35161 tcagtttttt caaaagtacg gataaggcgg caattttgcc ggtgtgtatc atcaggtggg
    35221 aaatgtggtt ttcccggata aagcgggtaa gcgcgtagag cgaaaacagc ccgttgaacg
    35281 tgtgaaggcg cgttgtgaaa acggaagaaa cttcggaaaa gcgtctgcgc atcaattcat
    35341 catttttatt gacggcggta aacatggtgc agccccgaag tcccaatgct tttgaaacat
    35401 catagacata ttgttcgccc ccaccccaaa tcttggccga tacgatattt aatatcctga
    35461 attctttttc catttaaaat tttccgccgg atttgtacag ataatatagt ttgacatatt
    35521 taatcatcgt gtaatagctg tggttgacgg acataatcca acccattttt ccatcaagaa
    35581 accctttgtt caggatataa attttgaaaa aaccccaaat cggacggagg ataatgtccc
    35641 taacgaaact cacgggcttt ccctgctccc ggtatttctc ggccgagatg gaagtatatt
    35701 tgttgaactt gttgaaatat tgttcccagt tgtcgtacgt gtaatggtac ataaaatgtt
    35761 tcagacggcg ttcggggtag ggggtctgta cggtttcgtg tactttgcct tgcaccgaac
    35821 cgcctttttt cggcatcaga cggcatacgc tgtcgggacg catcgcgccg tgtgtggcgg
    35881 gatggttggg gaaaaggttg cggcgttcga caaaataggc ggcataatcg ccggttcgga
    35941 caatttttga gatttcatca gatagttccg gcgtgcagcg ttcgtctgca tcaatcagga
    36001 aaacccattc tccgcctgcc tgttcgatgg caaatgtttt ttgcgctccg aaatccccgt
    36061 tcaaatgccg tctgaagact tttgcaccca aaccctcggc aatttcggca gtgttgtcgg
    36121 cgctatggtc gtcgataacg ataacttctt tatcgaaacg gcaactttca atacattccc
    36181 gaatgtggtt tgcttcgttt ttggcaacaa tcaatacgct gacttttttc attatcaagg
    36241 tttctcaaag cattttggtt tcacagggcg gcaaaaaagc ccaaacatac gtccgggctt
    36301 gggaattggt gggtccggtg gggttcgaac ccacgaccaa gggattatga gtcccctgct
    36361 ctaacccctg agctacagac ccgtcaagag taggcgcgta ttgtatagca tagcggtggg
    36421 attgacaaac ttatttcatc cgatgattaa gacgcagttg cttcccccga aggcaaacga
    36481 cgagctggcg gcaatgcgtt tttcggtttc ccagccgccg ccggtcaggt taatggcggg
    36541 caggtcgggg tcgttctgcc cgtcccaaag ccggggcgga agtttccctt cgggattgct
    36601 ttgccggtcg gcaatgcccc acgcgaacgc ggcttcgatt gcgcccgccg cgcccagcgt
    36661 gtgtccggtt tgcggcttgg cggacgtgca gggcgtattg ttgccgaaaa ccgctgcaac
    36721 ggcgcggctt tccatactgc cgttgtggtg cgtcccggtg ccgtgcagat taatccggcc
    36781 gatgtcttcg ggcgcaaggc cggcgtgctg caatgccgtc tgaaaggcga ggattgcgcc
    36841 tttgcgtcgg ggcggggcgt ggaaatatgg taggcatcgc tgcttgcgcc gtaacccaga
    36901 agctgcatac cgccggaaaa atccgcatcg cgcgtcatga tgaatacggc tgcgccttcg
    36961 ccgatattga tgccgtcgct gttggcgccg tagccgacga gttcggcgta aattttcgca
    37021 ccgcgccgtt tggcgtgttc caattcttcc aatacgaaaa tccccgcgcc ttttctccga
    37081 tgaccagccc gtcgcggttc gcgtcgtatg gacgcggggt ttcttccggt tcgccgttgc
    37141 ggcggctggc ggcatagagc gagtcgaaaa catacacttc ggacgggcaa aattcttcgc
    37201 cgccgcccgc cagcatcata tcggtcagac cgtatttgat ggcctcgtaa gcataaccta
    37261 tgccttggct gcccgaagaa cacgcgctcg atgtcgggat gatgcgtcct tattaatccg
    37321 aaaaagatgc cgatattggc gtcggcggtg tgcggcatca tacgcacata ggtattggcg
    37381 ccgaagttgc gcgacgtgcc ggtcagcaac aattcgccca catctccgat gtctttggtg
    37441 ccgccgccgg aagagccgca ggcaacgccc atccgtccgt cggtaatgct ttcgtctccg
    37501 agcaaaccgg catccgccag cgcctgctcc gccgcatcga cgcacaggta cgacacgcgc
    37561 cccatacttc tgagccgctt gcgcgtccaa tgtttcggcg gcgcgtaatc ctcaatcggc
    37621 gcacccagtt gcgcttccaa ttcggggaaa cgttcgcgcc aatccatata tttgacggcg
    37681 tttttttcgg ctttgaatgc tgcctgtatg ctttgccaat cccggccgaa ggcggtaatg
    37741 ccgcctatgc ccgttactgc gacccttctg gtattcaaca caaacctccg ttcaccgcaa
    37801 tcacctggcg cgtgatatac gccgcttttt catccatcag gaagtgcact gcgtgcgcca
    37861 cttcttccgg cagtcccata cgcgcggcgg ggacggcttt caaaatttct ccgacgggta
    37921 cgttttcatc gacaatctcc gtatcgatca aacccggcgc cacgcagttg acggtgattt
    37981 tgcgtttcgc cggttcgacc gccaaggctt ttgccgcgcc gataatgccc gcttttgacg
    38041 cgctgtaatt gacctgcccc cggttgcccg tcaggccgga caccgatgcc atacacacaa
    38101 tccgtccggc tttgcggcgg cgtatcatcg gcataaccag cggatgcagt acattgtaaa
    38161 aaccgtccaa attagtccgc ggcaccacat cccaatcgtc gtctgaaaac gcggggaagg
    38221 cattgtcgcg cgtcaggccg gcgttcaaca ccacgccgta atacgcgccg tttgcttcga
    38281 tgtcggcggt cagaatctcg cggcaggctt cgcggtcgga cacgtcaaac tgcaacacgc
    38341 gcgcatttct gcccaaagcg cggatttctt ccgccacggc ttcggcttcg tcgcggcggc
    38401 tgcggcagtg gacggcgata tcaaagccgt cgtccgccaa aaccgagtgc ggcggctttg
    38461 cctatgcctc tgtttgagcc ggtaatcagg acggtttcag tcatggttgt tctccgtatg
    38521 tgtgcctgtt cagacggcgt cagttgttcc gtcatggtgt tcggggctgt acacgttgag
    38581 tgaggcgcgt gccaaaatgc cgtctgaaag gagtgttccg gacgaagttt ccggcgcatc
    38641 cgtccaacgc agttcgcagt cggacacgcc cataccccgg catcttggat agacatatgc
    38701 gccgttgccg gcaaatgtgt tccgacaggg acggattggg cgaagatttc aagtttgcgc
    38761 gtgccgggca ggaagccgag ccggaccggc cgtgcgtttt ttcgggcttg gatgccggca
    38821 tatgcgccga cagcctgcgc catcagttcg ataaatgccg tatagggcag catgccgccg
    38881 gccagcagga tgtggtcggg ttttacctgc gcccctgctt cgacaaaatc atcgccgtat
    38941 cgggtaatgc ggtctatcag aaccatacgc ccgctgtgcg gcagcaggga ggcggtgtcg
    39001 gtaatcgggc attatcaatg ataaatgtaa attcaggcta attcgggcgg tttgccgagg
    39061 ggcggcgttg tcggaaaaaa tggcgtggat gcgtttgaat ttcggcattg tcagggggat
    39121 gggctttggt tagaatgcgg cacagtgtaa atttttgaaa gaaactatgc cgccgattat
    39181 ttgccggttc cacttcgaca tcgccgactg acgggtttcg ggcggcaaaa tgcgcgatat
    39241 ggcccagtgg aggcagtggg cggaatgtcc ggattttgcc gacggtttgc ccgatgtcag
    39301 gcgggaattg ccgttcctgc ccgctatgcg gcgccgccgt ttgagtaagg cggcgcgttt
    39361 ggtatgcgac gcggcttggg acattgcctc cgcccatccc ggcagtccgg tagtttatgc
    39421 ctcgcacgac ggggaaatgg cgcgtagttt cgatttgtgg ttggaactgt tgaaatcgca
    39481 taccgtgtcg ccgacttcgt tcggtttgtc ggtgcacaat gcgacggcgg ggcaatggtc
    39541 gatattgagg cgggatatga gcgagcagac ggcattggcc gtgtgtgccg acggtgtgga
    39601 aacggcattg gcggaagcgg cgtccctgtt ggaagaggga tgcgggtcgg ttttggtttt
    39661 ggcggcggac gatccgctgc cggaaggata tgccgtatcg gcaacgcgcg cgccgtttgc
    39721 ttatgctttg gcgatggttc tgaccaaagg gacgcgttac agcctgactt tgtctgcttc
    39781 ggacgatatg ccgtctgaag ccggtatgct gcccgaggca tattggagcg gtttggaatg
    39841 ggtgcgtttt ctgctgaacg gcagtcggga atgccgccgg gtatatcgca atcgtgaatg
    39901 gttgtggcag cccgcaagct gcaggctgaa gatttccgca atgtccgcac cgtcaacgac
    39961 gtggtacagg cggtattgaa gattcaggcc ggctaatcgg gtatttctaa ttatttttca
    40021 gacggcatgt ttttatcgga tgccgtctga aaacgtttgt gcggattcaa aatgaaaccc
    40081 ggttttttcg gacatatttt tttgaccgta ttaagcattg cctatcccgc tttgtg
//
LOCUS       DAXDZG010000009        38940 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXDZG010000009 DAXDZG010000000
VERSION     DAXDZG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38940
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.009"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            212..2218
                     /locus_tag="ACFB78_000400"
     CDS             212..2218
                     /locus_tag="ACFB78_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC1268470.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2341..3432)
                     /gene="mltB"
                     /locus_tag="ACFB78_000401"
     CDS             complement(2341..3432)
                     /gene="mltB"
                     /locus_tag="ACFB78_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC1268471.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3580..5007)
                     /locus_tag="ACFB78_000402"
     CDS             complement(3580..5007)
                     /locus_tag="ACFB78_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC1268472.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERRNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5192..8896
                     /gene="mfd"
                     /locus_tag="ACFB78_000403"
     CDS             5192..8896
                     /gene="mfd"
                     /locus_tag="ACFB78_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC1268473.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDGIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9122..9424
                     /locus_tag="ACFB78_000404"
     CDS             9122..9424
                     /locus_tag="ACFB78_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC1268474.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9586..9969
                     /gene="panD"
                     /locus_tag="ACFB78_000405"
     CDS             9586..9969
                     /gene="panD"
                     /locus_tag="ACFB78_000405"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC1268475.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9991..10833
                     /gene="kdsA"
                     /locus_tag="ACFB78_000406"
     CDS             9991..10833
                     /gene="kdsA"
                     /locus_tag="ACFB78_000406"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC1268476.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10848..11288
                     /locus_tag="ACFB78_000407"
     CDS             10848..11288
                     /locus_tag="ACFB78_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC1268477.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11335..12621
                     /gene="eno"
                     /locus_tag="ACFB78_000408"
     CDS             11335..12621
                     /gene="eno"
                     /locus_tag="ACFB78_000408"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC1268478.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEVAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12789..13067
                     /gene="ftsB"
                     /locus_tag="ACFB78_000409"
     CDS             12789..13067
                     /gene="ftsB"
                     /locus_tag="ACFB78_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC1268479.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13108..13398)
                     /gene="yfaE"
                     /locus_tag="ACFB78_000410"
     CDS             complement(13108..13398)
                     /gene="yfaE"
                     /locus_tag="ACFB78_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC1268480.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13519..14652)
                     /gene="nrdB"
                     /locus_tag="ACFB78_000411"
     CDS             complement(13519..14652)
                     /gene="nrdB"
                     /locus_tag="ACFB78_000411"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC1268481.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14965..17244)
                     /gene="nrdA"
                     /locus_tag="ACFB78_000412"
     CDS             complement(14965..17244)
                     /gene="nrdA"
                     /locus_tag="ACFB78_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC1268482.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17775..18086
                     /locus_tag="ACFB78_000413"
     CDS             17775..18086
                     /locus_tag="ACFB78_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC1268483.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(18136..18246)
                     /locus_tag="ACFB78_000414"
                     /pseudo
     CDS             complement(18136..18246)
                     /locus_tag="ACFB78_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18349..19116)
                     /locus_tag="ACFB78_000415"
     CDS             complement(18349..19116)
                     /locus_tag="ACFB78_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC1268484.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(19204..20031)
                     /gene="mutM"
                     /locus_tag="ACFB78_000416"
     CDS             complement(19204..20031)
                     /gene="mutM"
                     /locus_tag="ACFB78_000416"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC1268485.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(20084..20749)
                     /locus_tag="ACFB78_000417"
     CDS             complement(20084..20749)
                     /locus_tag="ACFB78_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC1268486.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            20928..22904
                     /locus_tag="ACFB78_000418"
     CDS             20928..22904
                     /locus_tag="ACFB78_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC1268487.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            22972..23664
                     /locus_tag="ACFB78_000419"
     CDS             22972..23664
                     /locus_tag="ACFB78_000419"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC1268488.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            24138..25508
                     /locus_tag="ACFB78_000420"
     CDS             24138..25508
                     /locus_tag="ACFB78_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1268489.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            25741..26397
                     /gene="cmk"
                     /locus_tag="ACFB78_000421"
     CDS             25741..26397
                     /gene="cmk"
                     /locus_tag="ACFB78_000421"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC1268490.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26550..28235
                     /gene="rpsA"
                     /locus_tag="ACFB78_000422"
     CDS             26550..28235
                     /gene="rpsA"
                     /locus_tag="ACFB78_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC1268491.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28246..28560
                     /locus_tag="ACFB78_000423"
     CDS             28246..28560
                     /locus_tag="ACFB78_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC1268492.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(28756..29163)
                     /locus_tag="ACFB78_000424"
     CDS             complement(28756..29163)
                     /locus_tag="ACFB78_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC1268493.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            29313..30423
                     /locus_tag="ACFB78_000425"
                     /pseudo
     CDS             29313..30423
                     /locus_tag="ACFB78_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            30432..31259
                     /gene="fghA"
                     /locus_tag="ACFB78_000426"
     CDS             30432..31259
                     /gene="fghA"
                     /locus_tag="ACFB78_000426"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC1268494.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            31789..32940
                     /gene="zapE"
                     /locus_tag="ACFB78_000427"
     CDS             31789..32940
                     /gene="zapE"
                     /locus_tag="ACFB78_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC1268495.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            32999..33424
                     /gene="ndk"
                     /locus_tag="ACFB78_000428"
     CDS             32999..33424
                     /gene="ndk"
                     /locus_tag="ACFB78_000428"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC1268496.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            33568..34662
                     /gene="rlmN"
                     /locus_tag="ACFB78_000429"
     CDS             33568..34662
                     /gene="rlmN"
                     /locus_tag="ACFB78_000429"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC1268497.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            34665..35426
                     /gene="pilW"
                     /locus_tag="ACFB78_000430"
     CDS             34665..35426
                     /gene="pilW"
                     /locus_tag="ACFB78_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC1268498.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            35442..36707
                     /gene="ispG"
                     /locus_tag="ACFB78_000431"
                     /gene_synonym="gcpE"
     CDS             35442..36707
                     /gene="ispG"
                     /locus_tag="ACFB78_000431"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC1268499.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(36761..37375)
                     /gene="clpP"
                     /locus_tag="ACFB78_000432"
     CDS             complement(36761..37375)
                     /gene="clpP"
                     /locus_tag="ACFB78_000432"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC1268500.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(37471..38781)
                     /gene="tig"
                     /locus_tag="ACFB78_000433"
     CDS             complement(37471..38781)
                     /gene="tig"
                     /locus_tag="ACFB78_000433"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC1268501.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         9848 a        10752 c         9279 g         9061 t
ORIGIN      
        1 aacatatctg ctaatctagt acagacccgc cgaaattgtt ataatcgaaa aaattaaaaa
       61 tcaatacgga ttattctatc agattgattt atcgatattt attatatcat taatatagtt
      121 tgatttcaaa ccggcggaaa atgcgccgcc cgccgcagat gcgccctccc cgccatcggg
      181 gcgtttaacg ccggattgcc gatgcggtac aatggcagat atgaagaaaa ttacccctca
      241 aaacctgcgt cccctgcttt cggaaagctt gggacatacc gattttgtca acgtcctcaa
      301 cgcattgatt aaatttttgc gccgtggcgg caaaaaatgt gcgggggagc gtttcgacct
      361 gattatcgac acattcaaac aagacaggga attactgtcc cgcttcagcc ggtgttttta
      421 catctggctc gcgcaaatac acatttatcc ggcactcatc aaactcggca ttttctcgcg
      481 ccacagcttt gcccgggaaa tgggcatacg catatacgaa cgcttcaacc cgtcatataa
      541 agattttgcc aacttgggcg aagtcttcct ttatcttttc cattccgaaa acgacgacaa
      601 atggctgcaa acgctcaata tccgccaatg gctggtttta tacgaactca tccgtagcca
      661 cgccgagccg tccaaattgc agacggcggg catccgcctt gccgatgcgc gtttgcgcgc
      721 catcgaaatg ctgtctgtct ggacggcatc cgaagccatc gaacccgatc tcatccgtat
      781 cgccccgcgc ctgctggaag ccgattcttc cttcgtcgcc ctccaacgcg aaaccgccaa
      841 actggtcgaa cactaccgca acggcaccgc gccttacgac accgcccacc tcgaagtgat
      901 gttcgaccaa tgtttcagcc agattgacta tttgcgtcgc aaagggacgg gcgccggctc
      961 cggttcgtcg gtcaaagtcg cccacctgct cgaacgactc cggcagaccg taggccgtct
     1021 gaagctgctc acagacatac aaaccggtgc cggcaacagc aaccgcctga ccatcgccct
     1081 gatgaactcc ctcatctacg cggcggtcga acaatacagc acccgccacc tgcgccgcag
     1141 cagcatccgt atgctcgccc gcagcattac cgaaaacaaa agccaccacg gcgaacacta
     1201 catcacccgc aaccgcaaag aatatttcaa aatgttctac tcggcagcag gcggcggcgg
     1261 catcatcgcc ctgatggcgc tgctcaaaat ccgcatcggc acactcgacc tcagtccctt
     1321 cctcgcttcc ttgtcggctg ggttcaacta cggcatcggc tttatgatca tccatatgct
     1381 gcactgcacc gtcgccacca agcagcccgc gatgactgcc gccagctttg ccgaacaggt
     1441 cgatctcaac gaaggcggca aagcggtgga caacaaactc tccaagctcc tcatcgacgt
     1501 atgccgctcc caaagcgtcg ccgtcttcgg caacgtttcc atcgccatcc ttttggcgtg
     1561 cgccatatcg ttcggctatg cccatctgta ccggctgccc atactcgatg cccacaccgc
     1621 cgcctaccag ttcaaatcca tagacatcat caatcatcct accctatggt atgccgctat
     1681 tgcaggcttg tggctgttct gctccggcat catcgcaggt tttttcgaca accgcgccga
     1741 ctacctcaac ctgcgccaac gcctgccctt caatcccttg ctgcgtaaaa tcatgcgccc
     1801 cgggccccgc cgcgtcctcg ccgcctacat ccacaaacac tacggctcgc tggtcggcaa
     1861 cttcatcttc ggaatgctct tgggtatgac cggctatttc ggacacctcc tcgggctgcc
     1921 gctggacatc cgccacgtcg ccttttcctc cgccaacctc ggctatgccg ccgtcagcgg
     1981 caacgtcggc tttggtacat tcgtactcgg cattttcagc gtcctcgcca tcggcttggt
     2041 caacctctgc gtcagcttca gcctcgccct cttcgtcgcc ctgcgctcgc gcggcacaaa
     2101 aatcggcagc atccgcaacc tgatcaaaag tttttggaat cagattaaaa gcaatccttg
     2161 catacttttc ctcccgcccg ccaaaggaca gggacatcct ccttcggaca agccttgacc
     2221 ggcaatgccg tctgaaacgg gattcgcccc gaataccgcc ctgatgcggg aaatccccat
     2281 aaaaggatgc aaaaatgccg tctgaaccga aacgtggttc agacagcatt ttaaaaaaca
     2341 ttacaatccc gggccgccga gcgaattggc aatgtccctg accgccgtta catacatccg
     2401 gctgtggttg tactgccata ccgtataaaa attgttcaag cccaaataat attcaaacac
     2461 gccgggtgcg gtttccagtt tgaacaaaac cgccttttca tcatctgcga gcgtttcccc
     2521 ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc gtcagggcgg ttttttcgcc
     2581 aatgattgcc tgaacatccg cacccggcgc caacgtcgcc gacaccaaca ttttaccgcc
     2641 cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat gccgcgacat caccgacgtt
     2701 gccccatata tcccgatgtc cgtccccgtc ataatccacc gcccatttcc ggtagctcga
     2761 aggcataaat tgcggcatac ccattgcgcc cgcatagctg cccttaaagg cgaaaacatc
     2821 gccgccttct tcttttgcca gctttaaaag ctcgaccaat tctttttgga aaaacccggc
     2881 gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc gccacacgga agctgcccgt
     2941 atttttgccg taattcgttt caatcccgat aatcgccacg ataagctcgg caggcacgcc
     3001 gtatttttgc gccacatcat cgataaccgc gcggttttcc gcataaaacc tgcgcgcgcc
     3061 gtgaaatttc gccctgcccg aatttcccgt gcggaacaca taccacggac gcgatgtgga
     3121 gggtcggtgc ataatcttga cgatgtccgc cttgtaagcc gctttgtcaa aaaaatcctg
     3181 ccattccgcc tgggaaaaat cccctttccc gacttcatcg tccacaaaac ggcggacatt
     3241 tgcattggcg gcaaacccgc tgtcggatac cggtacggct gccgcgtcaa acgcggggcg
     3301 gctttctttt ttcatttcat ccgcgcgggg ggcttgggct tcatttgccc ggggtgtgcg
     3361 ggcctccatc gccgtacagg cagacaaagc cgccaaacaa attgccagcg gcagtatttt
     3421 tctcttttcc ataaacacat tccgaacaaa tagggtaagt gggaaagcgg cacaagggtg
     3481 cggcgcgaaa tgcggtatcc gccgcaatcg gcggttttgc aaaatgccgc gcgttgcctt
     3541 ttgcgccgcc cgaaatctgc ctgccgccgt atttgcccgc tatccgcaat atcggcagtc
     3601 caatatatct ttgcggatgt cgttcagcag gaaggctgtg gtgtcttcgt gttcctcctg
     3661 tacgaagaca aagagtcggg cgatgatttt gccgatgaag actttttgca gggcgcaggc
     3721 gtcggtcagg gtgtgttcct gcaggaagct gcggatgtct tcgggcaaag cgtagtcgcg
     3781 ggcaacggcg gcaaagcgga catcggtttg caggcggtcg agcagggttt ggtttttgtc
     3841 caacttaatg cctgcttctt tttcttcggc ggtggcgcgg acgaactggt cgtaaatttc
     3901 ggcataaagc atatcgttcg ggccttcaaa aatcgtgaag gggcggatat cgatggcgat
     3961 attgccggct gggtgtccgc gttcaaaacc cttcgcgccc aagagttttt gcaacatttg
     4021 cgcggcggcg taagtgtatt ccgtggcgag ggttttgacg atgttcgcct ccatcaattg
     4081 atgggcgacg ggcgcgacgg gcgaaacgga atggcagacg tagcggtaaa ggatttcgga
     4141 aacctgatgg cggcgttgga tttcgcggcg ttcgtaatcg acgaatctga tatcgttgcg
     4201 gacatatcgg ttcaggttgt caaggatgta ttccataatg ccgtgcgtca tgccgatcag
     4261 ttgcaggcgg ctgcggataa agatgttttg gaacgcgcgc aaaccggcag cgtcgccccg
     4321 ggagagtttc atcacggcgg ttgcaggcat ttcggcatcg atgcggttga cggcgtaacg
     4381 gacggcgcgc aggccttcgg atgcgagggt ttcgcagcgg atgtatgttt tggggacgag
     4441 cagcaggtcg atgactttgg cgagtttgcc gtttctgcgc tctttggcgg caacgaggag
     4501 gaagtcgctt tgcgaattgc cctgccagta tttcgcggcg ttgacgtaaa tggtttgttc
     4561 gtcggtatat tcgtagcagg actgcatttc gcgtgcaatc gccgcgccgg aggtttcggg
     4621 ttcggtaacg cccaaaccgc cgccttcgcc tttgaaaatc atgtccaaac cttgtgcgac
     4681 ttgcgcttcg ccgccgaact cttgcagagg ctgcaacacc agcgcgcctt cgatgccggt
     4741 acgcagcgta acgggcacgc cgtaatgccc cgcaatcctt aggacttctt ggatttcaaa
     4801 ctgactgccc ttgcgcccgc cgtgtttttt gtcgaggaag ggcaacagca aacccgcctg
     4861 cttcaaggca agccatttgc cttcgggcag gtatcgcatc aggtcgatgc cgtctgaaaa
     4921 aatgcggcgg aatgcggatt cgatgtgctt taaaaaagca gccgtgtcca tggttgacgg
     4981 ctgcgcgctc ggttcggtgt gtatcatcgg cttcctctgt cggttcccat taatcggcgg
     5041 ccggtcaaac cgcctgccac agtttagagt taattttcta aactttacca caaagtgcac
     5101 cgggcaacaa tccgccggcc tttcagacgg catcgcgcac cctcacgtgc taaaatgacc
     5161 gtttgcatca ctgtccgccg attgccgcac tatgacctgc cccatcccca aaccccgtga
     5221 aaaatcccgt tggttcaatc tttcgcaagg ctcgctgccc ttggctttgg cgcgttattt
     5281 gccgcacaag cggctcaaag ccgtgctgac ccaagatgcg gaacaggcgt tgcgccttca
     5341 gacggcatgg cggtttttcc gtccgcacga cacggcggtg ttcctgccgg actgggaaac
     5401 gctgccttac gagcgttttt ctccgcatca ggatttggtg tcggagcggc tgtcggcgtt
     5461 gtggcagatt aaaagcggcg cggcggacgt gttgttcgta ccggttgcca cggcgatgca
     5521 gaagctgccg cccgtgccgt ttttggcggg gcgcacgttt tggctgaaaa cggggcagac
     5581 tttggatata ggccgcctga aaaccgattt ggtggatgcg ggctacaatc atgtttccca
     5641 cgttgtcgcg gcgggcgagt ttgccgtgcg cggcggcatt gtcgatttgt tcccgatggg
     5701 cagcgaaacg ccgtaccgca tcgatttgtt tgacgatgaa atcgacggca ttaaaacctt
     5761 cgataccgac acgcagcgca ccatttcccc cgtttccgaa atccgcctgc tgccggcgca
     5821 cgagttcccc accgacagcg aggcgcaaaa aatcttccgc agccgcttcc gcgaggaagt
     5881 cgacggtaat ccgaacgatg ccgccgtgta caaagccgtc agcaacggcc acttcggcgc
     5941 gggcgtggaa tattatctgc cgctgttttt tgaaaacgag ctggaaacgc tgtttgacta
     6001 tatcggcgaa gatgcgctgt ttgtctcttt gggcgatgtt catgccgagg caaaccgctt
     6061 ttggaacgac gttaaatcac gttacgccat ggcgcagggc gacgaaacct atccgccttt
     6121 gcttccacag catttgtatc tctctgccga tgtgttcgca ggccgtctga aaaactacgg
     6181 acaagttctg cctgatgttt ccggcaaggc acactccctg cccgaccttg ccgtcaaccg
     6241 ccaatccgac gatcctttgc aggcattgaa ggattttcag acggcctttg acgggcggat
     6301 tttgctgtgc gccgaaagtt tgggacggcg cgaaaccatg ctcggtttct tgcagcaaaa
     6361 cggtttgaaa gccaaacctg tgtccgactg gcagggcttc ttgtcggcgc acgagccgct
     6421 gatgattacg gtcgcgccgt tggcttacgg attcaaactg ggcggactgc aatcttcaag
     6481 ccaacagcaa actgttcccg cctccgaggg agaaggcaaa gcggttaccg accaaaccga
     6541 attttccgca tccgcaacaa accctctccc cagccctctc ccgcaggaga gggaacaaag
     6601 tgccgccgcc gtttcagacg gcctgaaagc agccgccgtt tcaaccgaaa gcagcctgta
     6661 tctcgtcgca agtgatctgc acgggcaaac ccgacagcaa tctgccccct cccccgtggg
     6721 ggagggttgg ggagagggca aagcggttgc cgctcaaagt gccatcgccg tcatcaccga
     6781 atccgatctt taccaatacg tcgcccgttc gcgcgtccac aaccgccgta agaaacacgc
     6841 cgccgtttca gacggcctgt tgcgcgacct tgccgaaatc aatatcggcg atcccgtcgt
     6901 gcacgaagaa cacggcatcg ggcggtatac gggcttggta acgatggatt taggcggcga
     6961 aaccaacgaa atgatgttgc tcgaatacgc gggcgaagcg cagctttatg tgcctgtttc
     7021 gcaactgcat ttaatcagcc gctactccgg tcaggcgcat gaaagcgtcg ccctgcacaa
     7081 gctcggcagc ggcgcgtgga acaaggcgaa gcgcaaagcc gccgaaaaag cgcgcgatac
     7141 cgccgccgag ttgctcaacc tctacgccca acgcgccgcc caatcgggac acaagtttga
     7201 aatcaacgag tcggactatc aggcgtttgc cgacggcttc ggctacgagg aaaccgaaga
     7261 ccaggccgcc gccatcgccg ccgtgattaa agatttgacg caggcgaagc cgatggaccg
     7321 acttgtgtgc ggcgatgtcg gtttcggcaa aaccgaagtc gccctgcgcg ccgcgtttgt
     7381 ggcggtgatg ggcggcaaac aggtcgccgt actcgccccg accacgcttc tggtcgagca
     7441 gcacgcgcaa aacttcgccg accgtttcgc cgatttcccc gtaaaagtcg ccagcctttc
     7501 gcgtttcaac aacagcaaag ccaccaaagc cacgctcgaa ggcatggcgg acggtactgt
     7561 cgatatcgtt atcggtacgc acaaattggt gcaggacgac ataaaattca aaaacttagg
     7621 tttattgatt atcgatgaag aacaccgctt cggcgtgcga cagaaagagc agctcaaacg
     7681 cctgcgcgcc aatgtcgaca tcctaaccat gaccgccacg ccgattccgc gcaccctcag
     7741 catggcgctc gaaggtctgc gcgacttctc gctgattacc actgcaccga gccgccgcct
     7801 cgccgtgaaa acctttgtca aaccgttcag cgaaggcagc gtgcgcgaag ccgtgttgcg
     7861 cgaactcaaa cgcggaggac aggtattttt cctgcacaat gaagtagata cgattgagaa
     7921 tatgcgcgag cggctggaaa ccctgctgcc cgaagcccgt atcggcgtgg cgcacggaca
     7981 actgcgcgag cgcgagctgg aacaagtcat gcgcgacttt ttgcagcaaa gattcaatgt
     8041 gttgctctgt tccaccatca tcgaaaccgg catcgacatc cccaacgcca acaccatcat
     8101 catcaaccgc gccgacaaat tcgggctggc ccaactgcac cagcttcgcg gacgcgtcgg
     8161 ccgcagccac caccaagcct acgcctacct gctcacgccc gaatacatca ccaaagacgc
     8221 agaaaaacgc ctcgacgcca tcgcggcggc agacgaactc ggcgcaggtt tcaccctcgc
     8281 catgcaggat ttggaaatcc gaggcgcagg cgaaatcctc ggcgaaggac aatccggcga
     8341 aatgatgcag gtcggcttca cgctctacac cgaaatgctc aaacaggccg tgcgcgacct
     8401 caaaaaaggc cgccaacccg acctcgacgc accgttgggc attaccaccg aaatcaaact
     8461 gcacagcccg gcattactgc ctgaagacta ctgccccgac atccacgagc gcctcgtcct
     8521 ctacaaacgc ctcgccgtat gcgaaaccgt gcagaaaatc aacgccatac acgaagaact
     8581 cgtcgaccgc ttcggcctga ccgaacaacc cgtcaaaacc ctcattgaaa gccaccacct
     8641 gcggcttgcg gcaaaagaac tgggtattga tgccatcgat gccacaagtg aagccgtaac
     8701 cgttaccttc ggcaaacacc actgtatcga cccgacgggg attatcctgc tgattcagac
     8761 ggataaaaaa taccggctgg caggggcaga caaattgagg tttgcagcgg aaatggaaaa
     8821 catcgaggtc aggatcaata ccgtcaaaac cgtactaaaa accctgcaag gcaaacgcct
     8881 gcccaaagga aattgatgta acgatgccgt ctgaagaaca ccgttcagac ggcatacaat
     8941 cccccaatcc tcaccaaaag gaacaatcat gtcgattttt cacccctatt tccaacaact
     9001 gtccaccgaa ggttttgacg gagaagaaga tgtcctctgg gaagacgagc tgaccgtcaa
     9061 aggcaatctt gtcgaggtat tcctgtaggc agaaaatgaa aacagcctgc caaccgaaac
     9121 attggatgca tttgccgcct tcctgtccga attggaaaaa gccgacgatg ctgcacgcgc
     9181 cgcactgacg gaatatttga aacgggacag ccgctatatc gactttcaca ccgaagatac
     9241 ccaaacaagc cgtgatgcgg caaaatttgt ccgcactatg cagttgatct atatcagctt
     9301 atgggcaaaa aatccggctt ttgccgttat ggattatatg cccgccaata tggaaagcga
     9361 cgaaatcctt gccgtcaaac tgcatttgga cggcagcatt ttctcaatcg attgggagag
     9421 ttgagccgga ttgccaatcg gcaaaaatga aatgccgtct gaaaacagct tccctgcccg
     9481 aagccttgcc attatgaatt tgaaggaaac tccactataa tacggcattc agattttcag
     9541 acggcatcac gcccgtcaga ccgcacacaa accaaaagga aatacatgtt ccgtactata
     9601 cttggcggaa aaatccaccg cgccaccgta accgaagccg atttaaacta cgtcggcagc
     9661 attaccgtcg atcaagacct gttagacgcg gcaggcatct gccccaacga aaaagtcgcc
     9721 atcgtcaaca acaacaacgg cgaacgtttt gaaacctata ccattgcagg gaaacgcggc
     9781 agcggcgtga tttgcctgaa cggtgctgca gccaggctgg tacagaaagg cgacatcgtc
     9841 atcattatgt cttatatcca actttccgaa ccggaaatcg ccgcacacga acccaaagtc
     9901 gtcttagtgg acggaaacaa taaaatccgc gacatcatct cctacgagcc gccgcacacc
     9961 gtactgtaat cccgcaaacg gacatcgatt atggatatta aaatcaacga catcaccctc
    10021 ggcaacaatt cgcctttcgt cctattcggc ggcatcaacg ttttagaaga tttggattcc
    10081 accctccaaa cctgtgcgca ttacgtcgaa gttacccgca aactgggcat cccctatatc
    10141 tttaaagcct ctttcgacaa ggcaaaccgc tcgtctatcc attcctatcg cggcgtaggc
    10201 ttggaagaag gcttaaagat ttttgaaaaa gtcaaagcag agttcggcat ccccgtcatt
    10261 accgacgtac acgaacccca tcaatgccaa cccgtcgccg aagtgtgcga tgtcatccag
    10321 cttcccgcct ttcttgcgcg gcagaccgat ttggtggccg caatggcgga aacgggcaat
    10381 gttatcaaca tcaaaaaacc ccagttcctc agcccttcgc aaatgaaaaa catcgtggaa
    10441 aaattccgcg aagccggcaa cgggaagctg attttatgcg aacgcggcag cagcttcggc
    10501 tacgacaacc tcgttgtcga tatgctcggt ttcggcgtga tgaaacaaac ctgcggcaac
    10561 ctgccggtta ttttcgacgt tacccattcc ctgcaaaccc gcgatgccgg ttctgccgca
    10621 tccggcggtc gtcgcgcaca ggctttggat ttggcacttg caggcatggc aacccgcctt
    10681 gccggcctgt tcctcgaatc gcaccccgat ccgaaactgg caaaatgcga cggccccagc
    10741 gcgctgccgc tacacctttt agaaaatttt ttaatccgca tcaaagcatt ggacgattta
    10801 atcaaatcac aaccgatttt aacaatcgag taacacggtt tcgccttatg atgcagactt
    10861 tccgaaaaat cagcctgtat gccgcaacct tgtggctcgg tatgcagatt atggcaggtt
    10921 atatcgccgc accggtgctg ttcaaaatgc tgcccaaaat gcaggcgggc gaaattgccg
    10981 gcgtattgtt cgacatcctc tcttggagcg ggcttgccgt ttggggcacg gtactggctg
    11041 ccgcctttgc cgccctaacc cggcggcaaa ccgccctgct gcttttttta ttgtccgccc
    11101 ttgccgccaa ccaatttttg gttacacccg ttatcgaggc actgaaatac gggcatgaaa
    11161 attggctgtt gtcggttgca ggcggatcct tcggaatgtg gcacggtatt tccagcatga
    11221 ctttcatggc aaccgcccta ctttcagcag ttttaagttg gcggctttcc ggcaaagagg
    11281 ccgtctgaag ccctcccatt ttttacctcc cttcacttca cttggagaac attcatgagc
    11341 gcaatcgttg atattttcgc ccgcgaaatt ttggactcgc gcggcaaccc cacagtcgag
    11401 tgtgatgtat tgctcgaatc cggcgtaatg ggacgtgcgg ccgtaccgag cggcgcatcc
    11461 accggtcaga aagaagcttt ggaacttcgc gacggcgaca aatcccgcta ttccggcaaa
    11521 ggcgtattga aggcggtcga acacgtcaac aaccaaatcg cccaagccct catcggtatc
    11581 gatgccaacg agcaatctta tatcgaccaa atcatgatcg aattggacgg tactgaaaac
    11641 aaaggcaatt tgggtgcgaa tgcgactttg gcggtctcta tggcggttgc acgcgccgct
    11701 gccgaagact caggcctgcc gctttaccgc tacttggggg gcgcaggtcc gatgtccctg
    11761 cccgtaccga tgatgaacgt catcaacggc ggcgaacacg ccaacaacag cctgaacatc
    11821 caagagttta tgattatgcc cgtcggcgca aaatctttcc gcgaagcgtt gcgctgcggt
    11881 gcggaaattt tccacgcctt gaaaaaactg tgcgacagta aaggcttccc gaccacagtc
    11941 ggcgacgaag gcggtttcgc ccccaacctg aacagccaca aagaagccct gcaactgatg
    12001 gtcgaagcgg ccgaagtcgc cggctacaag gcgggcgaag acgtattatt cgcattggac
    12061 tgcgcgtcca gcgagttcta caaagacggc aaataccact tggaagccga aggccgctcc
    12121 tacaccaacg cggaatttgc cgaatacttg gaaggcttgg ttaacgaatt cccgattatt
    12181 tccattgaag acgggatgga cgaaaacgac tgggaaggct ggaaactgct gaccgaaaaa
    12241 ttgggcaaaa aagttcaatt ggtcggcgac gacttgttcg taaccaatcc gaaaattctt
    12301 gccgaaggca tcgaaaaagg cgtagcaaac gcattgctgg tcaaagtcaa ccaaatcggt
    12361 actttaagcg aaaccctgaa agccgtcgat ctggcaaaat gcaaccgcta cgccagcgtg
    12421 atgagccacc gctccggcga aaccgaagac agtaccattg ccgacttggc agtcgccacc
    12481 aactgtatgc agattaaaac cggttctttg agccgttccg accgcatggc gaaatacaac
    12541 caactgctgc gtatcgagga agaattggcg gaagccgcct actaccccgg caaagccgca
    12601 ttctaccaac tgggcaaata aataaaaaag tggatgtatg tttcaacaca cagggcaaca
    12661 catgaagcgt cgccctgtgt gttgacctga tttggaagag gttacacctc tcccaaataa
    12721 agtctaattt taccgccacg aaggacggac atccgagtgg tggggtttca accagtaagg
    12781 aaacgttgat gaagtgggta actgtcgttt tatccttcgc acttgtctgt tgccaataca
    12841 gcctgtggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa cagattgccg
    12901 ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc gccgaagtct
    12961 atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa ctgggttata
    13021 tccaagacgg cgaaaccttt taccgactca tcaggcataa ccggtaatac cgtcaaaaag
    13081 ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca gcccctcatc
    13141 ctcccccgcc aaaccgcaat ccagcctgac atccccctcg acgcaacagc agcacggcag
    13201 tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc ttccctccaa
    13261 aatctttacc cggcacgatc cgcaatatcc gcttcggcac tgatattcca ccatatgccc
    13321 cgtccgttcc aagccttcca acagggtctc accctccaag agctcaaaaa ggcccttatt
    13381 cgtaccaatg cgcgccattt ccgaccaatc aaaaataaaa ggaacaataa ccgatcctac
    13441 ctgctgtttt tcccatcata caacacacaa atgccgtctg aaacatccgg cttcagacgg
    13501 cattttttca aaaaacattt acaactcaaa atcgcccaag tcatccgtat tcacttcaga
    13561 atcgatttga ccaatcaaat aagaggatat ttccacttcc tgcggcgcga cctgtacgtt
    13621 gtcggacgac agccacgcat taatccacgg aatcgggttt tgatttgcgc cttcaaatcc
    13681 ggccggcagc cccaccgcct gcatacgcag attggtaata tattcgacgt attgggataa
    13741 gatttctttg ttcaaaccga tcatcgaacc gtctttaaac aaatatgccg cccattcttt
    13801 ttcctgttcc gccgcttttt tgaagagttg gaaacattcg tcctgcaact cggcggcaat
    13861 ttccgccatt tccggatcat caacacccga acgcatcaga ttaagcatat gctgcgtgcc
    13921 ggtcaggtgc agggcttcat cgcgggcaat cagtttgatg attttggcgt tgccttccat
    13981 caactcacgc tcagcaaaag caaacgagca ggcaaatgaa acgtagaaac ggatggcttc
    14041 caacacattg acgcacatca ggcagagata gagttttttc ttcaacccgc gcaaagacac
    14101 agtaacgggt tttccgccga cattgtgcac cccttcgccc aacaggttgt aatattgggt
    14161 gtattcgatt aaatcatcat aatagcaggc aatgtcttcg gcgcgggcgg taatgtattc
    14221 gttttcgaca atatcatcaa acacgaccga cggatcattc acaatattgc ggatgatgtg
    14281 ggtatagctg cgcgagtgga tggtttcgct gaagctccac gtttcaaccc aagtttccag
    14341 ctcgggaatc gaaaccaaag gcagcaaggc aacattcgga ctgcgccctt ggatggaatc
    14401 gagcagtgtt tgatatttca gattgctgat gaaaatatgt ttttcgtgtt cgggcaggtt
    14461 ggcgtagtcg atacggtcgc gcgacacgtc gatttcttcc ggccgccaaa aaaacgacaa
    14521 ttgtttttca atcagttttt caaatacctc gtatttctgc tggtcataac gggcgacatt
    14581 taccggctga ccgaaaaaca tcggctcttt cagcgcgtcg tttttggttt tgggaaaggt
    14641 gctgtatgac atgactaggt gttcgcagga cataacgtct ctctttttaa aaattagtcg
    14701 acttttctat taatttttaa ttcaccatat aaaacatgtt gaattctatc aataaagtgc
    14761 catcctaaaa tagcaaatac taaaagaaca ctaataaata tataaaattt atattcaact
    14821 aagaatgacc gaatgacatc catacttttc cttttctgga aaataacaac ttagcctcat
    14881 atagtagtag gcactaattt aagaaaatcc ttgaagcctc ctactatcta gaagccttgg
    14941 atatatttca aaaattaacc tgttttaaat cttacaagcc ccaccagcgc agccgtcatc
    15001 ttgaatatcg gtctgcgtgt cgtccgcgcc gtcgcgggtg ttgtggtagt acagggtttt
    15061 gacgccgtat ttgtaggcag tcagcaggtc tttgagcatt tgtttcatag aaactttgtt
    15121 accttcgaat ttgcccgggt cataagcagt gttggcggaa atcgcctgat cgacgaattt
    15181 ttgcattacg ccgacaagtt tcaggtagcc ttcgttgccc ggaagctgcc acagggtttc
    15241 gtaggcattt ttcaaggttt caaactccgg cacgacttgt ttcaaaatgc cgtctttcga
    15301 tgctttgacc gttaccaatc cgcgcggcgg ctcgatgccg ttggtggcgt tggcgatttg
    15361 cgagctggtt tcagacggca tgagcgcggt cagagtagag ttgcgcaggc cgtatttgac
    15421 gatgtcggca cgcaggcttt cccagtcgta atgcaaaggc tcgccgcaga cggcatccaa
    15481 atcttttttg taggtgtcga tgggcagttt gccttgcgaa taaacggttt ggttaaagag
    15541 cgtgcacgct ccgtattctt tggcaaggtt tgccgatgct ttgagcaggt aatactgtat
    15601 ggcttcaaag gtacggtggg tcagaccgag cgcggaaccg tcgctgtagc ggacaccgtt
    15661 tttcgccaga tagtaggcgt agttgatgac accgatgccg agcgaacgcc ggtccatagt
    15721 agaggtacgc gcggcttcta ccggatatcc ctgataatct aaaagtgcat cgagtgcgcg
    15781 cacggtcaaa tcggcaagcc cttccaattc gtccaagctg tttaatgcac ccaagttgaa
    15841 ggcggacagt gtacacaggg ctatttcgcc gtcagggtcg ttgatgttgt ccagcggttt
    15901 ggtcggcagg gcgatttcca tacacaagtt ggactgatga acaggcgcaa cgcgcggatc
    15961 gaacgggctg tgcgtattgc agtgatcgac gttttgaatg tagatgcgcc cggtcccggc
    16021 acgctcctgc atcagcgtgg aaaacaggtc ggcagccgga atgatacgct tgcgtatgtt
    16081 cgggtcttgc tcgtatttcg tatagagccg ctcaaattcg tcttggtcgg caaaaaacgc
    16141 ttcgtacaac cccgaaacct cgttgggcga aaacagcgta atgttgccgc ccttaatcag
    16201 gcgggtgtac agcaggcggt tgatttgcac gccataatca agctggcgga tacggttgtc
    16261 ttccacgccc cggttgtttt tcaacaccag caggctttcg gcttcaatat gccacaaggg
    16321 gtagaacaag gttgccgcgc cgccgcgcac gccaccttgc gaacaagatt tgaccgccgc
    16381 ctgaaacatc ttaaagaagg gaatgcagcc ggtatgccgg gcttcaccgc cccggatttc
    16441 gctgtccagc ccgcggatac gtccggcatt gatgccgatg cccgcgcgtt gggaaacgta
    16501 tttcacaatc gcgctggtag tggcattgat ggaatccaaa ctatcgtcgc attcaatcag
    16561 cacacagctt gagaactggc gcgtaggcgt acgcacgccg ctcataatcg gcgtgggcaa
    16621 cgatactttg aatgtggaaa cggcatcgta aaaccgtttg acgtaaccca agcgcacctc
    16681 tttcgggtat ttgctgaaaa ggcacatcgc caccaaaaca tataaaaact gcggcgtttc
    16741 gtaaatctgg cgggtaacgc ggttctgtac cagatatttg ccttcgagct gtttgacggc
    16801 ggcataggaa aaggacatat cgcgttcgtg gtcgatatag gcgttcagtt cgtcaaattc
    16861 ttcgcggctg taatcctcaa ggatatgcct gtcgtatttt ccggcatcgg tgagcttttt
    16921 aacatggttg taaaggtgcg gcggctcgta ttcgccgtag gctattttac gaagatggaa
    16981 aatcgccaaa cgtgcggcaa ggtattggta gtccggggta tcttccgaaa ttaaatctgc
    17041 agcggctttg atgatggttt cgtggatgtc gtcggtgcgg atgccgttgt agaactggat
    17101 gtgcgatttc aactcgacct gcgacacgga aacattttcc aatccttccg ccgcccaagt
    17161 aacgacacgg tgaatcttat ccaaatcaat ggcttccaat cttccgtctc gtttggttac
    17221 ttttaaatca gtcggtgtat tcatcgcttc ctcttccact cttgatattc aagacacggt
    17281 cttttcaaat aaattaaggc agacaatata gtggattttt agcattttct ccagtcttga
    17341 caacgattgc atttttcagt ttcaggcaat gcgcgataaa acccgcctgt cgtatttttc
    17401 ctataacatt tgttttatct gataattctt taccgataaa aaacgggtaa attttttgcc
    17461 ttttgaccgg ctccggctac aaggcgatga aataaggatt ttccgacgaa aaaggaaaac
    17521 ttccggtttt ccgcccctgc gaattgttaa attttgcaaa gtatgatttt tgccacgccg
    17581 ccgccgacaa attccatttt cttaccgatt ggaatttatt attgagatta atatgttatt
    17641 tgaatttgca tatcaaacgg caagttttgt cggctgaatg agtctgaaac tgccgacagt
    17701 ttgcccgttc catccttcca tcctatactg gaaggaatta caaactgaaa ggatcgtcat
    17761 gtccgtcaat aaagatgctt gccatactgt tgtctgcaat actgggactg gtatcaacaa
    17821 ctgccgctgc cggtacgtca gaacccgccc accgacatac caaacatatc agcaaggcaa
    17881 acaagcagat gctgcacccc gaatgcagga aatatttgga acgccgtgcc gcgtggtacc
    17941 gatcgcaagg caacgtgcag gaattgcgcg aaaacaaaaa ggcgcgcaaa gcattccgca
    18001 ccctgcctta tgcggaacag aaaatccaat gccgggcggc ttatgaggct ttcgatgatt
    18061 tcgacggcgg caggttccgc cgttaatccc atataaaata tgctgtctga acacaagtta
    18121 agatggcatt ttccatagat atagtggatt aaatttagac cggtacggcg ttgcctcgcc
    18181 ttgccgtgct atttgtactg tctgcggctt cgccgccttg tcctgattta aatttaatcc
    18241 actatacaac aaactatcct tacggcattt cttttgaggt actcccgtta ccgcagcctt
    18301 aaggcatccg caaaagcgaa tcataccgcc acattccgac aaccgctttc agacggcaac
    18361 atccgcatcg cctgcgacaa cggcacggat gctttcttcg attttatctt ttaaagcata
    18421 acggtcttcg ctttccgccg cgtccgccac gcaaacgaaa tcgactttga tagtcagttt
    18481 tttcatagac acgattcgcc acaggcaagt cggcaaaccg acatcggcat acgacggacg
    18541 ggccgtcctt tttcccgttt cgtcataata acgcagtgcg accgccaaaa cctttgcccc
    18601 cgcatcgatg gcggattgga acagcgcagc cttgaacggc aggagcccca gtccggagga
    18661 agttcgcgcc tcagggaaaa aactgacgtt ttgaccgcgt tgcaaggttt cgcagacggc
    18721 gcggttaatc ggttcgatgt cgcgccgcga attgcggttg atgaacaccg ttcccgcgtt
    18781 ctgccccatc ttgcccaata ccggccagct tttgatttcc tgcttggcga taaagctact
    18841 cggataaacc gcgctcatcg cgaaaatatc cagccaggac acgtggttgg cggcaaccaa
    18901 gacaccgttc ggatgttcgg gtgcgggtct gcccacctcc aatccgatat ccaaagccgc
    18961 caaagcgccc ttgcccaacg ctataactgc ccgattgcgc gacttggggc agccgccgtc
    19021 aataccgcgc aggtttttgc cggttttgaa cagccagacc gtcaagcggc acaagcggcg
    19081 cagacgtgta aaaaatgaag ctttatttga agacatgggc aattctttcc cgataatcgg
    19141 tgtttgttgt tgaaacgttc ggacggcata ccgataaaat gccgtcagaa accgttttcc
    19201 gccctatttc tgacagttcg tgcaataaaa cgtgccgcgc tgccccaaag tttctttcac
    19261 aaccaaaccg ccgcaccgga ggcacggctg attgtgccgc ccgtacaccg tatattcctg
    19321 ttgaaagtag ccgcttttgc cgtcgctgtc cacaaaatcc ctcagcgtac tgccgcccgt
    19381 ttcaatggcg cgctgcaaca ccgctttgac ggtttcaacc aaaaccgcgc actctttctt
    19441 ttttaggcgg ttggcaggac ggtggggcga aatgcccgcc ctgaacagac tctcgttggc
    19501 ataaatattg cccacgccga ccacgacggt attgtccatc agagcgagtt tgaccgcgcg
    19561 tttctgcgcc ttcagccccg catacagata atccgtgcaa aatgcctccg acaaaggctc
    19621 cggccccagt ttttccaaca gcggatgacg ttcttcgatt ccctcatacc aaagtatcgc
    19681 gccgaacttt ctcggatcgc ggtaacgcat gaccgtgccg tctgaaaaca caatatcgac
    19741 gtgatcgtgt ctgtccggcc tgccgatacg tccgtccgac ggcgtaaaga tccgcaagct
    19801 gcccgacatc cccaagtgaa tcagcaaaat ccccgtttgg aaacggacaa tcagatattt
    19861 ggcacgcctg ccgcaggaca aaacctgccg gccggacaaa atctccccca aatcgggatt
    19921 gatctgccag cgcagcttca gttggcgcaa tatcacggct tccaccgttt ttccttcaat
    19981 atgcggcgcg atgccgcgca acgtcgtttc cacttccggc aattccggca taacccctcc
    20041 cgacatttct tctgacagat gccgtctgaa agacggccgt cccttaatcc gcaacccttg
    20101 ccgcacccgc cgcaagggct ttgccgcccc aatacccgtc ccacgcgggc aggggcgtta
    20161 cgcccgccgc ccttttgacc aaaaccaaac cgtacactgc ggcacattgc ggccaccaac
    20221 ggtcgcccgc cttttccata aaccgccaaa agcgtatttg cccgagcgac gaaacgggag
    20281 gcagatacac catgaatttc ccaaattcaa tatcgaaacc ggcatcggca agctgccttt
    20341 tcaactcggg cagcggcaga caaaaccgtt tttccggcag acggacaccg tcaaaccaac
    20401 ggcagaatcc ccaaagcgaa tacggattga aacccgtcag catcaaacgt ccggacggtt
    20461 tcaatatccg gtgcgcttcc gacaggattt gcgaaggtac gccgcattcc agcgtatgcg
    20521 gcaaaagcag catatccgca aaaacatccg ccaaagccat attctccgcc gacatcgaca
    20581 tatctcgcgg cacacagaca atgtcttcag acaggctcag ccactgaccg cccacctgaa
    20641 ccgcacacat tcccgaaaaa cggtatgacg ccacataccg attaaagaag tcctgttcca
    20701 attttgcaac ataccgcccc atcgccgtat cttcaaacca tgcatccata ttgcgtccgt
    20761 ttcaaacaga ttgcctgccg atattttatt ccaaacagga tttctgtcaa aaaacacatc
    20821 ggccgcccat ttccgaatcc gcataaagtt cctgtaaaac ttgacgcttt ttcagtcaaa
    20881 cagtaccatc ggacgataaa atatgtttat tccgccgagt ataaatcatg tccaaactca
    20941 aaaccatcgc cctgaccgca tcaggtctgt ccgtttgtcc gggtttccta tatgcccaaa
    21001 acacctcatc acaccaagtc ggtttagcga ttatgcggtt aaactcttca atactcgacc
    21061 tgccaccgac aaaacaatat ttccaatccg gcagcctgtg ggacgagctg cgccaaggct
    21121 tccggatggg cgaagtcaat cccgaactgg tacgccgcca cgaaagcaaa ttcatcgcaa
    21181 gccgcagcta tttcgacagg gtcgtcaacc ggagccgacc ctatatgtac catatcgcca
    21241 acgaagtcaa aaaacgcaat atgcccgccg aagccgccct gcttcccttc atcgaaagcg
    21301 cgttcgtcac caaagccaaa tcacacgtcg gcgcatcggg cctgtggcag ttcatgcccg
    21361 ctaccggcag gcattacggc ttggaaaaaa caccggttta cgacggcagg cacgacgttt
    21421 acgcagccac cgatgccgca ctcaactatc tgcaatatct ctatggactg ttcggcgact
    21481 ggccgctcgc ctttgccgcc tacaactggg gtgaaggcaa cgtcggacgc gccgtcaacc
    21541 gcgcccgcga ccaagggctc gaaccgacct acgaaaacct gcgtatgccc aacgaaacgc
    21601 gcaactatgt ccccaagctg ctcgccgtgc gcaacattat tgccaccccc caatctttcg
    21661 gcatgaatat cagcgacata gacaacaaac cctattttca ggcagtcgaa ccgggccgtc
    21721 cgctcgacaa cgaagccatc gcccggcttg ccggcatcac gcaaagcgag ctgctcgccc
    21781 tgaatcctgc attcaacgtc cccgcgttca tccccaaaaa caaacgcaaa ctgctgcttc
    21841 ctgtcgcgtc cgtccaaacc ttccaaagca actacctcaa cgccgcaccc gacagcctgt
    21901 tttcatggga agtctatacg cctgccgcca aaaccagcct gtccgacatc tcgacggcaa
    21961 ccggcatgag cattgccgac atcaaacgcc tcaacaacct gaacggcaac cttgtcaacg
    22021 caggacgcag catccttgtc gccaagaacg gcaagaccct tcatacggca tcggaatccg
    22081 tcgtttccat cgacatcgac aatacgcccg acacctaccg ttccaatatg ccggcaggca
    22141 cggtgaacgt cagcattgcc cgaatccaac ccgccgccgc acagacagcg gacattaccg
    22201 tcgcaccttt gccgcaagaa accgtccgta cggaacccga tccccttgtc cgcattgccg
    22261 aacccgccct tgcgacagcc gcagcgcaac ctcaaaccga aaaacagact gccatgccgt
    22321 ctgaaaccca aaccgcaaca ctcgcgcagg tcgtcccccc aaacgacatg caggcggcag
    22381 acgaactcat gcagcttgtt gcccgaaaca acctgcgccg gcaggctgaa gaaaccatct
    22441 ccgccgtcat cggcacgcct gacacagttg ccgaacacaa catttcctca tctccgcaac
    22501 ataccgttgc cgccgacggc aaacgccggg cacgtttgga aacgcgcgta gccaaagctg
    22561 ccgacggcga agccgaaacc tccccgctcc atgccagcat ccaccgcgtt gtagaaggcg
    22621 acaccctgtt caacattgcc aaacgctaca acgtcagcgt agccgacctg attgtcgcca
    22681 acaacatcaa aggcaacacc atccaaaaag gacaggtact ccgcctggcg caggcagccc
    22741 ctgcccaaac ccgtattgaa aaagtatcct acaccgcgcg caaaggcgac accttcaaaa
    22801 gtatcgccgc gcgcttcaat atccatatcg acgacatccg ccggctcaat cccaacctga
    22861 acaccatcaa tccgggacag agggtcaaac tgattggaag ctgattcgga tacggcacat
    22921 gacaggactt tctcagtcct gtctttttca tcccacattt cccataccat catgaaactc
    22981 atcaaatacc tgcaatatca aggcatagga agccgcaagc agtgccaatg gctgattagg
    23041 ggcggctacg tctccatcaa cggaacctgc atggacgaca ccgatgcaga catcgattcc
    23101 tcatacgtcg aaacgttgga tattgacggg gaagcagtaa ccgtcgttcc cgaaccctat
    23161 ttctacatcc tgctcaacaa gcctgaagat tacgaaactt cgcacaaacc gaaacactac
    23221 cgcagcgtat tcagtctgtt ccccgacaat atgcggaaca tcgatatgca ggcggtcggc
    23281 aggctggatg cggatacgac cggcgtattg ctaattacca acgacggcaa actgaaccac
    23341 aacctgactt cgccgagcag aaaaattccc aagctatacg aagtaacgct caaacaccct
    23401 acaggagaaa cgctctgcga aactttgaaa aacggcgtgc cgctccacga cgaaaacgaa
    23461 accgtttgtg ccgccgatgc cgttttggaa aatccgacca ccctgctgct gaccattacc
    23521 gaaggaaaat accaccaagt caaacgcatg gttgccgccg ccggcaaccg cgtgcaacac
    23581 cttcaccgcc ggcgattcgc acatttggaa acagaaaacc tcaaacccgg ggaatggaaa
    23641 tttatcgaat gtccgaaatt ctgaaacaaa attccataat ttccctatat tcaaccatat
    23701 gcttattaaa taagcagttt tacaaaaatt aactcaaaaa acagttagtt tacaatataa
    23761 tccgcttgtc taagtaacgc ctgttcaagc aggattatga gcaagacgtt ttccccgtaa
    23821 tgtgttttgc catctatact tccccccttg tatagatggt tttctatttc aaattccctc
    23881 tctttcatca tcattgaaat gctatcatta atatgattaa caaaatttca cttccgtaca
    23941 ccgatgtaga aaaataacag attaatctgt tcataaactc cgattcttcc tcatcggttt
    24001 atactcatca gaaatattca ctgaaatata tgcattttac atggaaaatc actaggcagt
    24061 tgaaaaacat tcagtcaatt tctgttaaga atcacgtgta aaatttccgc tttatctcaa
    24121 tcggacacgg tttcaatatg tcttcccctt caaataccaa ccgtcaaacc tggtccagcc
    24181 gtttgactta tatccttacc gttgccggcg cgactgtcgg tttcggcgcg acgtggcgtt
    24241 tcccgtattt ggtcggtgaa aacggcggcg gcgcgtatgt gtttttattc tgtatcgcga
    24301 tgctggttat cggcatcccg atgattttgg tggaaaacgt catcggacgg cgcaaaggcg
    24361 tgaacgcgct ggatgcgttc ggcggcccga tgaacggcaa acctgttgcc aaaatttgga
    24421 aactggtcgg ccggatgggg ctgctcggcg cgttcggcat catggcttat tatatggtac
    24481 tcggtggctg ggtaatcagc tatatcgtta atattattgg aggaaatttg aatatttcca
    24541 gccccgtcga cggtgtggtt acaaaaggct tctttaccga acacattgaa aacagccctt
    24601 gggaaattgc gttttatacg ctgctttttg tcgccgtgaa ccaatggatt ttggtcaaag
    24661 gcgttatcgg cggcattgaa aaaacggcga aatacctgat gcctctgctg tttttgttcc
    24721 taatcgcgat ggtcgtccgc aacgttaccc ttccgggcgc aatggaaggg gttgctttct
    24781 atctgaaacc tgatttcagc aagattaccg ccgaactgtt cgtcttcgtt ttgggacagg
    24841 tattttttgc cctgagcctg ggtttcggcg tgatgattac cttatccagc tatctggata
    24901 aaaacgaaaa tctggttcag acggcagtta tcacggcaat taccaatacc atcatcgctg
    24961 tacttgcggg ctttatgatt ttcccgtcgc tcttcagctt cggcgtcgcg cccaattccg
    25021 gcccgacttt ggtgttccaa agtttaccga ttgtgttctc acatatgtgg gcaggacctg
    25081 tgttcgccgt cattttcttc tctctgctcc tgattgccgc gttgacaact tcgctgacga
    25141 tttatgaagt gttgattacg accattcagg aaaaaaccaa aatccgccgt accgccgcaa
    25201 tcacgattgt attgtccgtc atcttcgttt tcggcaacat cccgtctatt ttgagctatg
    25261 gcccgtggaa agacgtttcc gtgttcggca aaaatatttt cgatgccttc gactacatca
    25321 gcggcaacat cttgtttatg ctgaccgcgc tcggttccgc attgtttgtc ggttttgtga
    25381 tgaaggacga agcgaaggaa gaattgcttt ataaaggcaa ccatacgacg gtcaatattt
    25441 ggtttgccta tgtgaaatac cttgtgccgc tggtgattct gctgattttc gtcagcaacc
    25501 tgttctaatc cgcagcaatc aatgccgtct gaaggtcata cctctttcag acggcattgc
    25561 attgatgccc gccacgccgg aatccgaacc ggcttgccgg cttctgtcgg acgctttccg
    25621 ggcaggcggc gttccgtctg cttagacaat cgtcctttaa aacaggtaga atccgcccca
    25681 acgggaaaca cacccttcag acggcaaaac ccatacccca aaccatcagg aatccccctt
    25741 atgaacagac aaaaagtcat cgccatcgac ggtccgggcg catcgggcaa aggcacggtc
    25801 gccgcccgcg ttgccgccgc attgggatac gattatctcg ataccggcgc gctctaccgc
    25861 ctgaccgccc tatatgcaca aaaacaaggc gtggaatggc acgatgaaga aaacgtttcc
    25921 gcacttgcca aaaaactgcc cgccgtattt tcaggcaacc gcatcctact tgatggcgaa
    25981 gacgtttcag acggcatccg gacagaagcc atcggtatgg gcgcatccgc agttgcgcaa
    26041 tggcccaaag tccgcgccgc actgctgcaa cgccaacgcg attttctgac cgaaaaagga
    26101 ctggttgccg acggacggga caccggatcg gtcgtcttcc cccaagccga actcaaaatt
    26161 tttctgacag ccgaatctaa aatccgtgcc gaacgccgcg ccaaacaaat cggcattccc
    26221 tgcgaaggtt tcacattcga gcgcatcctg tccgacatcg agaccagaga cgaagcagac
    26281 cgaaaccgca aggttgcccc cctgaaacaa cagcccgatg ccctgctttt ggatacgagc
    26341 cgcctaacta tagaagaaac tgtaaaaaaa gtgcttgatt ggtatcgtaa agtttaaatt
    26401 ttcaggtata atcgcccgca ttgcgcttca gacagcatga atttccatgt gccgtctgaa
    26461 accttattta cccgcctgcc gtgccaagga ctgcagacac ccaccaaccc aacccgcacc
    26521 ccttggcggt gtaccgaaaa gagttatata tgtctatgga aaattttgct cagctgttgg
    26581 aagaaagctt taccctgcaa gaaatgaacc cgggtgaggt gattaccgct gaagtagtgg
    26641 caatcgacca aaacttcgtt accgtaaacg caggtctgaa atcagaatcc ctgatcgatg
    26701 tagctgaatt caaaaacgct caaggcgaaa ttgaagttaa agtcggcgac ttcgttaccg
    26761 ttaccatcga atccgtcgaa aacggcttcg gcgaaaccaa actgtcccgc gaaaaagcca
    26821 aacgcgcagc cgattggatc gctttggaag aagccatgga aaacggcaac atcctgtccg
    26881 gcatcatcaa cggtaaagtc aaaggcggcc tgaccgttat gatcagcagc atccgcgcat
    26941 tcctgccggg ttctttggtc gacgtacgtc ccgttaaaga cacttcccat tttgaaggca
    27001 aagagatcga attcaaagtg atcaaactgg acaaaaaacg caacaacgtc gttgtttccc
    27061 gccgcgccgt tttggaagcc actttgggtg aagaacgcaa agccctgctg gaaaacctgc
    27121 aagaaggctc cgtcatcaaa ggcatcgtca aaaatatcac cgactacggc gcattcgttg
    27181 acctgggcgg catcgacggc ctgctgcaca tcaccgattt ggcatggcgt cgcgtgaaac
    27241 acccgagcga agtcttggaa gtcggtcagg aagttgaagc caaagtattg aaattcgacc
    27301 aagaaaaaca acgtgtttcc ttgggtatga aacaactggg cgaagatcct tggagcggtc
    27361 tgacccgccg ttatccgcaa gccacccgcc tgttcggcaa agtatccaac ctgaccgact
    27421 acggcgcatt cgtcgaaatc gaacaaggca tcgaaggttt ggtacacgtc tccgaaatgg
    27481 actggaccaa caaaaacgta cacccgagca aagtcgtaca actgggtgac gaagtcgaag
    27541 tcatgatttt ggaaatcgac gaaggccgcc gccgtatctc tttgggtatg aaacaatgcc
    27601 aagccaatcc ttgggaagaa tttgccgcca accacaacaa aggagacaaa atctccggtg
    27661 cggttaaatc cattaccgat ttcggcgtat tcgtcggcct gcccggcggc atcgacggtc
    27721 tggttcacct gtccgacctg tcttggaccg aatccggcga agaagccgta cgcaaataca
    27781 aaaaaggaga agaagtcgaa gccgtcgtat tggcaatcga tgtggaaaaa gaacgcatct
    27841 ccttgggtat caaacaactg gaaggcgatc ctttcggcaa cttcatcagc gtgaacgaca
    27901 aaggttcttt ggttaaaggt tccgtgaaat ctgttgatgc caaaggtgct gttgttgccc
    27961 tgtctgacga agtagaaggc tacctgcctg cttccgaatt tgcagccgac cgcgttgaag
    28021 acttgaccac caaactgaaa gaaggcgacg aagttgaagc cgtcatcgtt accgttgacc
    28081 gcaaaaaccg cagcatcaaa ctttccgtta aagccaaaga tgccaaagaa agccgcgaag
    28141 cactgaactc cgtcaatgcc gccgccaatg cgaatgccgg taccaccagc ttgggcgacc
    28201 tgctgaaagc caaactctcc ggcgaacaag aataaggttg cagacatgac aaagtctgag
    28261 ttaatggttc gcttggcaga agtatttgcc gccaaaaacg gcacgcatct tctggcaaaa
    28321 gacgtagagt acagcgtaaa agtcttggtt gacaccatga cccgatcgct tgcccgaggt
    28381 caacgcatcg aaatccgcgg tttcggcagc ttcgatttga accatcgtcc tgcccgcatc
    28441 ggtcgcaatc ccaaaaccgg cgagcgcgtg gaagtacctg aaaaacatgt accccacttc
    28501 aagcctggta aagaattgcg cgagcgggtc gacttggctt taaaagaaaa tgccaattaa
    28561 accttagcat caaaacgccg ctggtacgcg gcgttttttc tttggtttaa cttcatccgc
    28621 tgcttcaata ccttaagcca agcaagcaac ggattagagc gtggattttt taccattgct
    28681 tacaatccgc tttttaaaca acaaattgtt gatttctatt acgaacagga caaaatcctg
    28741 cttattgcac taaaactaag ccgtttcagg aatttgcggc aaatttacag cttttaccga
    28801 gcctaatgct ttcgcttttt ggtaaaacgc caatttgtat tcaagcaaat ctaaataacg
    28861 ttttaattcg gcaatttgac acttcacatt ttctatttga ttttcaaaca aggaaaggcg
    28921 ttcttcaatg gtatcgtcgc caatgacggt acattccgca aagcgtttga tgtcttttaa
    28981 gctcattccc gtatttttca agcattgcaa taagtacaac cattgcaaat cgttatcggt
    29041 aaaacaacgg ttaccgtatt catcacgtcc gatattaggc aacaagcctt ctttgtcgta
    29101 aaagcgtaaa gtgtgggcgg agatgcctat tttttcggca gctttggcag tggtataagt
    29161 cattttccct ccctctaaac aaaaaacggt aaaaaaacac ttgccttaga gtgaactcta
    29221 aaatgtaaac tgttgctatg ttgctcaggc aaggctattt tgttaatgaa ttaagaggaa
    29281 agacaatgga aatgaaacaa accgattcaa ccatcaaatc tcgtgcggcg gtggcattcg
    29341 cccctaacca acccttacaa attgtggaaa tcgacgtgga aatgccgcgt aaagacgagg
    29401 tgttaattcg taacacccac actggcgtgt gccatactga tgcatttacc ttatcaggaa
    29461 gcgatcctga aggcgtattc cctgtggtgc ttggacacga aggtgcgggt gtggtcgttg
    29521 ctgtgggcga gggtgtgtca agcgtaaaac cgggtaatca cgtcattccg ctttacaccg
    29581 cagaatgtgg cgaatgtgag ttttgcctct cgggtaaaac caatttatgc gtctcagtgc
    29641 gtgatacaca aggtaaaggc ttaatgccag acggcacgac gcgtttttct tatcaaggtc
    29701 agccgattta tcactatatg ggctgttcga ctttcagtga atattctgtt gttgccgaag
    29761 tttcactggc gaaaatcaac ccgaaagcca accacgaaca agtatgtttg ctcggctgcg
    29821 gcgttaccac tggtattggt gcggtacata acacggcaaa agtacaagaa ggcgattctg
    29881 ttgccgtgtt tggcttggga gcgattggtt tggctgtggt gcaaggtgcg cgtcaagcca
    29941 aagccggtcg cattatcgcc attgatacca atcctgcaaa attcgagttg gcaaaacagt
    30001 gttggtgcaa ctgactgttt gagcccgaaa gattacgata aaccaatcaa agatgtgttg
    30061 ttagacatca acaaatgggg cattgaccac acctttgaat gtatcggcaa tgtaaacgta
    30121 atgcgtcagg cattagaaag tgcacaccga ggttggggac aatccatcat catcggcgta
    30181 gcggatgcag gacaagaaat ttcaacgcgt ccgttccaat cggtaacagg ccgtgtttgg
    30241 aaaggctcgg catttggtgg tgttaaaggc cgctccgagc ttccgaaaat ggtggaggat
    30301 tcaatgaaag gcgatatcca attagaacca tttgtgaccc atgcaatgac ccttgatcaa
    30361 atcaatgaag cctttgagtt aatgcacgaa ggtaaatcga tccgcgctgt tattcattac
    30421 taaggtccac gatgaaactg attgaacaac atcaaatttt tggtggttcg caacaagttt
    30481 gggcgcatca tgcccaaacg cttcaatgcg aaatgaaatt tgccgtctat ttgccggata
    30541 atcgggaaaa tcaaccgctt ggtgtgattt attggctttc cggcttgacg tgtacggaac
    30601 aaaatttcat taccaaatcg agcttccggc gttatgcggc agaacatcaa gtggttgtgg
    30661 tcgcccccga taccggccct cgcggagagc aagtgccgaa cggtgccgct tatgatttag
    30721 gacagggggc aggcttttat ttgaatgcga ccgaacagcc ttgggcggcg aattatcaaa
    30781 tgtatgatta cattttgaac gagttgcccc gtctgattga ggagcatttt cctaccaacg
    30841 gcaaacgttc cattatgggg cattcgatgg acggacacgg cgcattggta ttggcactac
    30901 ggaatcggga acattatcaa agtgtttctg ccttttcgcc tattttatcg ccaagcctcg
    30961 taccgtgggg agaaaaagcc tttactgctt atttagggaa agaccgtgaa aaatggcagc
    31021 aatatgatgc taactcactc attcaacaag gctataaagt gcaaggtatg cgtatcgatc
    31081 agggcttgga agatgagttt ttgccgacac aattgcgtac caaagatttt atcgaaacct
    31141 gtcgtgcggc aaatcagcca atcgatgtgc gtttccataa aggatacgat cacagctatt
    31201 acttcatcgc cagttttatt ggtgagcata tcgcttatca cgccgcattt ttgaagtaag
    31261 tcaaagagcg gtcagttttc aaagcagttt ggaatagccg gcacgagggc ggtaagaagt
    31321 gccggcataa acgtatgccg cctgaaccag aaggagccgg ccctacggat taccaaaccc
    31381 tgcccttgat ttgattagta tgatttttaa gcttaaaccc tgattgatca aacattaaaa
    31441 tcgcccgtat ttcctacttc actttcgggc ttcgtattat tcgttgccga atttgcataa
    31501 gcaacctgtt cctgttgtgt tttcgccccc tccaagctga cggtggcgta acagcttcgc
    31561 gattcaccat ctgtttcatt tgtcctcctt caatattaaa acgcagcctg aaataaaagg
    31621 cattttggct catatctgca ccatattaaa acgccgcctt tgcttatacc ccctttgtgc
    31681 gcgtcattat tcttttccac gggaaacgcc aagtttgaag gaaatcattt ataataccag
    31741 cggtaagcat tttctttctt agccgcaaga agtataacaa ggttaaatat gaataataga
    31801 gaccaacttt ttaaagcccc gccgtttgaa aaccacagcc cgctgacctg gtatcaggct
    31861 gcctcacaac tgcccaactt catccgcgac gacgcacagg cggccgccat cgaacacctt
    31921 gaccggcttt ggaccgaatt gatgatgttc aaacgcaaaa gaaaccgttt tttaggcagg
    31981 agtttgcgtt ccccgcaagt tcccaaaggg ctttatttct atggcggagt cggacgcggc
    32041 aagagctttc tgatggacgc ttttttcggc tgcctccctt accgccgcaa acgccgcgtc
    32101 cactttcatg cctttatggc ggaaatccac cggcggctga aagccctgaa aagcgaaagc
    32161 aacccgttga aatccgttgc cgccgagatt accaaagaaa cccgagtatt gtgttttgac
    32221 gaatttcatg tcagcgatat tgcggatgca atgattttag gccgtctgct ggaaaacctg
    32281 cttaacgagg gcgttgtttt ggtggcgact tcaaactacg cgccttccga actctacccg
    32341 caaggtcaaa accggagcgg ttttcttccc acaatcgcgc tcatcgagtc cagtctgacc
    32401 gtcttaaacg ttgacggagg ggaagactac cggctgcgta ccctccgccc cgccgagatt
    32461 ttctttacgc ctgccaatga agaaaatgag gcaaaactgg caaaactgtt caaagaaatg
    32521 acaggcatta ccgatttgaa ccccggcatc agcaccatcc acggccggga aattccccac
    32581 aaagccgagt ccggtcgtac catatggttt gatttccgcg cactgtgctt cagtccccgc
    32641 tcacagtccg actatctgta tttggccgaa cattatgaaa tggtttttat ttcaggtttg
    32701 gaacaactct caccgcaaga aaaggcggag gcgcgacggc tgacttggct gattgacgta
    32761 ctctacgatt tccgggtcaa actgtgtgcc accggcgcgg tagatgtcaa ccatatctac
    32821 acggaaggcg attttgccga agaatttacc cgcaccgcca gccggatggt cgaaatgcag
    32881 tccgaagttt atttggaaca gccgcacctg accttatctc ccaaggcttc aggcggataa
    32941 gttatttttt tggtagaata ccgatttgat tcttcttaag taaaaataag gatatagcat
    33001 ggcgattgaa cgtaccatat ccatcatcaa acccgatgcc gtcggcaaaa acgttatcgg
    33061 caaaatatac agccgctttg aggagaacgg tctgaaaatc gttgccgcca aaatgaagca
    33121 gcttaccctc aaagaagccc aagaatttta tgcggttcat aaagaccgcc ccttctatgc
    33181 cggattggtt gaatttatga ccggcggtcc ggttatgatt caagtactgg aaggcgaaaa
    33241 cgccgttctg aaaaaccgcg aactgatggg ggcgaccaat cccaccgaag ccgcagaagg
    33301 cacgatacgc gcggactttg ccacttcggt cagcattaac gccgtacacg gttccgacag
    33361 tgtggaaaat gccgctttgg aaattgccta ctttttcagc caaaccgaaa tctgcccccg
    33421 ttgatacaat acaccgccca attcctattc agacggcata aaatatatcc atgccgtctg
    33481 aaaactctgt tgcaaaagac ttcaaatcaa acttgcctgc cctgcaattt tttatttgaa
    33541 gccttaattt aagaaaaaca caaatacatg aaaaccaatc tgctcaacta cgaccttcaa
    33601 gggctaaccc gacattttgc cgatatgggt gaaaaaccat tccgcgccaa acaggttatg
    33661 cgttggatgc accaatccgg cgcgcaaaat tttgacgaaa tgaccgattt ggcaaaatcg
    33721 ttgcgccata aactgaacga acaggcaagc atcgaaattc ccaagctgat gatgtcccaa
    33781 gaatcttcag acggcactcg aaaatggctt ttggatgtcg gtacggggaa cggcgtggaa
    33841 accgtcttca tccccgaatc ggatcgcggc acgctctgca tttcctcgca agtcggctgc
    33901 gctttggaat gtacattttg ttcgaccggc cggcagggct tcaaccgcaa tttaaccgct
    33961 gccgaaatca tcggacagtt gtggtgggca aacaaagcga tgggcgttac accgaaaaac
    34021 gagcgtgtga tttccaacgt cgtcatgatg ggcatgggcg agccgatggc gaacttcgac
    34081 aatgtcgtta ccgccttgag catcatgctg gacgaccacg gctacggttt gagccgccgc
    34141 cgcgtaaccg tttccacttc gggtatggtt ccccaaatgg acaggttgcg cgatgtcatg
    34201 ccggtggcct tggcggtttc cctccacgct tccaatgacg aagtccgcaa ccaaatcgta
    34261 ccgttgaaca aaaaatatcc cttgaaagaa ttgatggccg cgtgccaacg ctatctggtc
    34321 aaagcgccca gggatttcat cactttcgag tatgtgatgc tggacggtgt caacgataag
    34381 gcacaacatg catacgaact tattgaactg gtcaaagatg ttccctgcaa attcaattta
    34441 atcccgttta atcctttccc taattcggga tatgaacgtt caagtaacga aaacatccgt
    34501 attttcagag atatcctgca acaagccgaa tttgtcgtta ccgttcgaaa aactcgtggc
    34561 gacgatatcg atgccgcctg cggacagttg gcaggtcagg ttcaggataa aacgcgccgc
    34621 caacaaaaat ggcagcagat tttaatcgga caacaggggt aattatgcct tttaagccat
    34681 ccaaacgaat ctctttatta cttgttcttg ccttgggcgc gtgcagcact tcctaccgcc
    34741 cctcgcgggc agaaaaagcc aatcaggttt ccaatatcaa aacccagttg gcgatggaat
    34801 atatgcgcgg tcaggactac cgtcaggcaa cggcaagtat tgaagatgcc ttgaaatcga
    34861 accctaaaaa cgagcttgcc tggctggtcc gtgccgaaat ctatcaatac ctgaaagtta
    34921 acgacaaggc gcaggaaagt ttccggcaag ccctctccat caaacccgac agtgccgaaa
    34981 tcaacaacaa ctacggctgg ttcctgtgcg gcaggctcaa ccgccctgcc gaatctatgg
    35041 catatttcga caaagccctg gccgacccca cctacccgac cccttatatt gccaacctga
    35101 ataaaggtat atgcagcgca aaacaggggc aattcggatt ggcggaagcc tatttgaaac
    35161 gttccctcgc cgcccagccg cagttcccac ccgcatttaa agaactggcg cgcaccaaaa
    35221 tgctggccgg gcagttgggc gatgccgatt actactttaa aaaataccaa agcagggtag
    35281 aagtccttca ggccgatgat ttgctgctag gctggaaaat tgccaaagcc ctcggcaacg
    35341 tgcaggcggc atacgaatat gaagcacaat tgcaggcaaa tttcccctac tcggaagaat
    35401 tgcaaaccgt cctcaccggt caataaacag attcaaacta tatgaacaca ctccaacgcc
    35461 gcaagacgca tcaagtcctc atcgatcata ttaccgtcgg ttcagaagca cccgtcgtta
    35521 tccaatctat gaccaacacc gacactgccg atgcaaaagc caccgcattg cagattaagg
    35581 aattgagcga tgccggctcg gaaatggtgc ggattaccgt caacagcccc gaagccgcgt
    35641 ccaaagttgc cgaaatccgc cgccgcttgg acgatatggg ctatgccaca ccactgattg
    35701 gcgatttcca ttttaacggc gaacgcctgt tggcggaatt tcccgaatgc ggcaaagcat
    35761 tgtccaaata ccgcatcaat cccggcaatg tcggcaaagg cgtaaaaggc gatgaaaaat
    35821 ttgcctttat gattcggact gctgctgaaa acgataaagc cgtccgtatc ggcgtaaact
    35881 ggggttcttt ggatcagagc ctcgccaaac gtatgatgga tgccaacctc gtttcttccg
    35941 cgccgaaacc gcccgaagaa gtgatgaagg aagcactgat tgtctccgct ttggaatctg
    36001 ccgaaaaagc cgttctattg ggactgcccg aagacaaaat catcctgtcg tgcaaagtca
    36061 gcgcggttca tgatttgatt caggtttacc gcgaactggg cagccgttgc gtctatccgc
    36121 tgcatttggg tttgaccgaa gccggtatgg gcagcaaagg cattgtcgca tcaacggcgg
    36181 cattatctgt tttgcttcaa gaaggaattg gcgacaccat ccgcatttca ctcactccgg
    36241 aaccgggcag cccgcgtact caggaggtcg tcgtcgggca agagatttta cagactatgg
    36301 gtttgcgctc gtttacgccg atggttaccg cctgcccggg atgcgggcgt accaccagta
    36361 ccgtatttca agagctggca caagatgttc aaaattacct gcgccaaaaa atgtctatat
    36421 ggcgtaccct ttatcccggg gttgaatccc tgaacgttgc cgtaatgggc tgcgttgtca
    36481 atggccccgg agaaagcaaa ttggccgaca tcggaatcag ccttcccggt acgggggaaa
    36541 cacccgtcgc acctgtttat gtagatgggg agcgcaaagt cacactgaaa ggtaataata
    36601 ttgcaagcga attcctagct attgtagagg aatatgttaa aaccaattat ggcaaaaaca
    36661 gttctaaacg caataaagga aaagtcatcc cgatacagtc tctataaaac aaatgccgtc
    36721 tgaacgtttc agacggcatt tgtttctctc agatttctta ttaaagccgc aaagaagcgc
    36781 ggttttccaa aacttggtcg atcaaaccat attcttttgc ttcttcggca gacatgaaat
    36841 tatcacggtc ggtgtcgcgc tccaaatctg ccaaatcgcg gccgcaatgt ttcgccatca
    36901 ggcggttgag tttttctttg attttcaaca actcgcgtgc gtggatttca atgtcggatg
    36961 cctgaccgcc caagccgccg ctgattaaag gctggtgaat cataatccgg ctgttgggca
    37021 gggcgaaacg tttgcctttc tcgcctgccg acaataagaa cgcgcccata cttgccgcct
    37081 gccccaagca caaagtcgat acatcgggct tgatgaaatt catcgtgtcg taaatcgaca
    37141 taccggccgt taccgagccg ccgggggaat tgatgtagaa gaaaatatcc ttatccggat
    37201 tctcactttc caaaaacaac agttgggcga ccaccagatt agcagactca tcggttaccg
    37261 ggccaaccag aaatacgatg cgctctttca aaagccggga atagatatcg aatgcacgct
    37321 caccgcgacc gctctgctcg ataacggtag ggacaagatg gttatcaaaa gacatttcgt
    37381 ctcctttcat gatggaaaag caccaaagcg ggctttaaaa acggcttcgg tgctttcaaa
    37441 aactgccttc agacagcatt ttcagaacaa tcaggcttgc gcgcccatca cttcgtcaaa
    37501 agacaaagct tttttgttta ctttggcttt gcccaaaacg aaatcaacaa cgttgctttc
    37561 tactgccaaa gaagtcgggg cttgcaggcg ggaagtatct gcgtagtacc agtcaatcac
    37621 ttcttgagga tcttcgtagc tttctgcgaa gttggcgaca acggctttga tttgctcttc
    37681 agtcggttcc agtttgtttt cgtcaaccag tttggccaaa atcagaccca aagatacgcg
    37741 acgttcggct tgttctttga acatatccaa aggcaaatcc aagttcgcgg catcggtcat
    37801 accttggtta acgaagtttt gtttcatttc gtttgccagg cgggcggctt cttcattgac
    37861 caaagcaacc ggaactttca actctacggc tttaatcagc gcgttcatta cggattcttt
    37921 ggtttgttcg ttcacgcggc gttccacttc gcggcttacg tttttcttca cttcttcacg
    37981 cattttggca acatcgccat ccgcaatacc caaggctttt gcaaaatctg catcgacttc
    38041 aggcagagtg ggctcggaaa cattgttcag cgtaatggtg aacacggcag ttttaccggc
    38101 aacatctttg ccgtggtatt cttcagggaa gttgacggta acgtctttac tttcgcccgc
    38161 tttcatgccg actacgccgg cttcaaattc aggcagcatt tgacctgcgc ccaatacgaa
    38221 ggcgtagttt ttggatgcgc cgccggcaaa aggttcgccg tcgattttgc cttcaaagtc
    38281 gatgatgacg cggtcgccgt ttcgggcttc gcggtcaaca tggttgaagc gggtacgttg
    38341 tttacgcagg atttctacag tttggtcgac ttcggcatcg ccgacggaag cggttacttt
    38401 ttcgacctct tgtgcagaca aatcgccgat aacgacttcg gggaacactt caaaaatggc
    38461 ggcaactttg aaagactctt tatcgtcttg ttcttcaacg ccttcaaaac gtggatagcc
    38521 tgccactttc aactcttggg caacggcaac atcgtagaag cggcgttgca ccagctcgtt
    38581 gatcacgtcg ttttgcgcgc tcgcaccgta catttgggca atcattttta aaggtgcttt
    38641 acccggacgg aaaccgtcga tttttgcacg gcgttgggtt tgtttcagtt ttttatcggt
    38701 ttctgcgttg atttcggacc aaggcaggga caacactact ttgcgttcca gattttctaa
    38761 aatttcaaca gttacgctca tcataagccc ttaaatttgt tgtgttgata aaatgataaa
    38821 ccttcttccc tacatgggga agcaaacagc gcaacggtac gatatttgaa ccgcattgcc
    38881 gcaaagggga aattttagct ggcaagtata tcacaatgtt tcgcctgaaa cataatatgc
//
LOCUS       DAXDZG010000010        37335 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXDZG010000010 DAXDZG010000000
VERSION     DAXDZG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37335
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.010"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..223
                     /locus_tag="ACFB78_000434"
     CDS             <1..223
                     /locus_tag="ACFB78_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268502.1"
                     /translation="TLNLFVEGFWIIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWD
                     RQMDPFYAHEIGNIWHGTHLDSSSEEYWK"
     gene            complement(280..438)
                     /locus_tag="ACFB78_000435"
     CDS             complement(280..438)
                     /locus_tag="ACFB78_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268503.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(442..1023)
                     /locus_tag="ACFB78_000436"
     CDS             complement(442..1023)
                     /locus_tag="ACFB78_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFC1268504.1"
                     /translation="MRNATSYGGNGVMWRGISSGRHVRGNYVLKASTAHNQAELERYV
                     HNPQVRAFLDFIAASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDA
                     SGRYQFMGYTWKGLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKL
                     GGIWASFPTAPNEYRQHKRSWGFVHNFFRQRGF"
     gene            complement(1029..4031)
                     /locus_tag="ACFB78_000437"
     CDS             complement(1029..4031)
                     /locus_tag="ACFB78_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC1268505.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMYQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     ASHMASESYQKAQSYREMAAQTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWKPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGISAAINARGENLKQEYANKSDKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDQTNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            complement(4038..5549)
                     /locus_tag="ACFB78_000438"
     CDS             complement(4038..5549)
                     /locus_tag="ACFB78_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC1268506.1"
                     /translation="MKFHIFRFPKPAKVPTVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGAWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(5721..6497)
                     /locus_tag="ACFB78_000439"
     CDS             complement(5721..6497)
                     /locus_tag="ACFB78_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC1268507.1"
                     /translation="MAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(6509..6934)
                     /locus_tag="ACFB78_000440"
     CDS             complement(6509..6934)
                     /locus_tag="ACFB78_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268508.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(6921..8477)
                     /gene="traN"
                     /locus_tag="ACFB78_000441"
     CDS             complement(6921..8477)
                     /gene="traN"
                     /locus_tag="ACFB78_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC1268509.1"
                     /translation="MICHFMLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVT
                     KDCWEYKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDV
                     DLRTLHQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKTINGV
                     PVTRDCWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTCQAREKQFEC
                     TEKGETTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQCSIKLDGAL
                     DSVFGGDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGT
                     ALSAAGGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQWLKCPQSEI
                     LVAMKRKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGKEQLKRGWGT
                     PENPKCEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKIREASVNGKN
                     LEVKNYYEYQ"
     gene            complement(8501..9745)
                     /locus_tag="ACFB78_000442"
     CDS             complement(8501..9745)
                     /locus_tag="ACFB78_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268510.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB78_000443"
     CDS             complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB78_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC1268511.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(10509..11627)
                     /locus_tag="ACFB78_000444"
     CDS             complement(10509..11627)
                     /locus_tag="ACFB78_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC1268512.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            complement(11624..12280)
                     /locus_tag="ACFB78_000445"
     CDS             complement(11624..12280)
                     /locus_tag="ACFB78_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC1268513.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(12289..12834)
                     /locus_tag="ACFB78_000446"
     CDS             complement(12289..12834)
                     /locus_tag="ACFB78_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC1268514.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(12834..13382)
                     /locus_tag="ACFB78_000447"
     CDS             complement(12834..13382)
                     /locus_tag="ACFB78_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268515.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB78_000448"
     CDS             complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB78_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC1268516.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(15996..16577)
                     /locus_tag="ACFB78_000449"
     CDS             complement(15996..16577)
                     /locus_tag="ACFB78_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC1268517.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(16574..17293)
                     /locus_tag="ACFB78_000450"
     CDS             complement(16574..17293)
                     /locus_tag="ACFB78_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC1268518.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(17303..18616)
                     /locus_tag="ACFB78_000451"
     CDS             complement(17303..18616)
                     /locus_tag="ACFB78_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC1268519.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRPIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(18618..19352)
                     /locus_tag="ACFB78_000452"
     CDS             complement(18618..19352)
                     /locus_tag="ACFB78_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC1268520.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(19354..20019)
                     /locus_tag="ACFB78_000453"
     CDS             complement(19354..20019)
                     /locus_tag="ACFB78_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFC1268521.1"
                     /translation="MKTEDMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     RGN"
     gene            complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB78_000454"
     CDS             complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB78_000454"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC1268522.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB78_000455"
     CDS             complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB78_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC1268523.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(20671..21192)
                     /locus_tag="ACFB78_000456"
     CDS             complement(20671..21192)
                     /locus_tag="ACFB78_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC1268524.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(21162..21623)
                     /locus_tag="ACFB78_000457"
     CDS             complement(21162..21623)
                     /locus_tag="ACFB78_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC1268525.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            22252..22554
                     /locus_tag="ACFB78_000458"
     CDS             22252..22554
                     /locus_tag="ACFB78_000458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268526.1"
                     /translation="MMTLDDDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEI
                     LFLMAQYGSEYWNSLEESFEEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            22551..25103
                     /gene="mobH"
                     /locus_tag="ACFB78_000459"
     CDS             22551..25103
                     /gene="mobH"
                     /locus_tag="ACFB78_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC1268527.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            25100..27142
                     /gene="traD"
                     /locus_tag="ACFB78_000460"
     CDS             25100..27142
                     /gene="traD"
                     /locus_tag="ACFB78_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC1268528.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSNKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            27132..27827
                     /locus_tag="ACFB78_000461"
     CDS             27132..27827
                     /locus_tag="ACFB78_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFC1268529.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPAYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(28257..28769)
                     /locus_tag="ACFB78_000462"
     CDS             complement(28257..28769)
                     /locus_tag="ACFB78_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268530.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(28860..29255)
                     /locus_tag="ACFB78_000463"
     CDS             complement(28860..29255)
                     /locus_tag="ACFB78_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1268531.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGFL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(29242..31164)
                     /locus_tag="ACFB78_000464"
     CDS             complement(29242..31164)
                     /locus_tag="ACFB78_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1268532.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(31241..31612)
                     /locus_tag="ACFB78_000465"
     CDS             complement(31241..31612)
                     /locus_tag="ACFB78_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC1268533.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            31808..33115
                     /locus_tag="ACFB78_000466"
     CDS             31808..33115
                     /locus_tag="ACFB78_000466"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC1268534.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            33108..33542
                     /locus_tag="ACFB78_000467"
     CDS             33108..33542
                     /locus_tag="ACFB78_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268535.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(33718..33987)
                     /locus_tag="ACFB78_000468"
     CDS             complement(33718..33987)
                     /locus_tag="ACFB78_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC1268536.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(34169..36631)
                     /gene="lon"
                     /locus_tag="ACFB78_000469"
     CDS             complement(34169..36631)
                     /gene="lon"
                     /locus_tag="ACFB78_000469"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC1268537.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            36883..>37335
                     /locus_tag="ACFB78_000470"
     CDS             36883..>37335
                     /locus_tag="ACFB78_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC1268538.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
BASE COUNT         9463 a         8945 c         8401 g        10526 t
ORIGIN      
        1 gacattaaat ctttttgttg agggtttttg gataattggc cttctccaag cagcagtatt
       61 tacgtttgtg gtagactgct caagtagtga tttaggttca ttaagtcaat ggcaaattgg
      121 aaatgactgg gacagacaaa tggatccatt ttacgctcat gaaattggta acatttggca
      181 tggaacgcac ttagactcga gttctgagga gtattggaaa taaaaaacag gagatttata
      241 aactcctgtt tttttatttc caaaagatgg ttaaattatc tatgtttcta gtaccttacc
      301 aattgaagtg atgatttgtt cgaaagctga tttgccttct tcggatacta gatcgttacc
      361 tccactataa ctcattctaa cttgataatt cccttcatca acctcttcaa tctgaacaac
      421 aatttgtgca atactcatct tttaaaatcc tctctgccta aagaaattat gtacaaaacc
      481 ccaacttcgt ttgtgttgcc ggtattcatt aggagctgtt gggaagctgg cccagatacc
      541 acctaatttc atgactgcct gctcgtaatc gccatttaaa atagcttcca ttgcaccttt
      601 tttagctaaa attaaggcga cagcagcttt gtcttgtgtg gcgggactaa aatccgtgaa
      661 gccatattgt tttgccagtc ctttccatgt gtatcccatg aattgatagc ggccggaggc
      721 atcagatgtg cttctactac catcggttcg agtaaaacta cgtctaacag agttacccgg
      781 gtgacgggag aagtccgtaa ttctataatt acctattgcc gtctgataac catgttttac
      841 accttctgat gccgcaataa aatctaaaaa ggcgcgcact tgcggattat gaacatatcg
      901 ctccaattcg gcttgattat gagcagttga agcttttaat acatagtttc ctctcacatg
      961 tcttccactt gatattccac gccacataac cccgttgcct ccatatgagg tggcattacg
     1021 caacggggtt attcctttgg gcggaataca gcggatccgc tagacttcga attccctgga
     1081 cgttgaggaa ttggtaggtc aaattcggat gctaaagccg ggttgcccaa atgggctgct
     1141 gcttgatccg acccagaacc tatgtatcct tttccttcac gaacgtcttt cattgcatta
     1201 tcgtatcttt caccgaaaga gttccagggt aaggcgtgag tattaattct catggttacc
     1261 ccattcgttt gatcttgagc atttttagca tgcgttgcca atctaccaac ttcttggcgg
     1321 tggctatttc taccttgatt aatatcgcct tcaaccttat cagatttatt tgcgtattcc
     1381 tgtttcagat tctcacccct agcgttgata gcagcactaa ttccatccgg aattataccg
     1441 gcctgatgag cttgggtttg aacatctcca aatgccgccc cagctccgga tgaaacttta
     1501 tcagagaagt gtttataagc ggcttgcgta ttgttttgag gtctatcttc aggggaaagg
     1561 tcattaggtt tccagcctga attttccgca gcagctttta aacgatcttg actatttaca
     1621 cctgcacgaa ttgcagcatt gtaggttcct ccctgtcctg cgtttaattc actcatagtc
     1681 aattcggggt tgtcttgtac caatgcattt gtgttatctt gggcaatggt aatgccatta
     1741 ccataagttt gagctgccat ttctcgataa gattgagctt tttggtagct ttctgatgcc
     1801 atatgcgagg cttctcttgc tttgctaaag ttagcttcgg cagtttgtgc caaactatta
     1861 ccagaattga gtgcaacttt atatgcttct gattgggaaa ctttgtctgt aaaattacta
     1921 tcaaagctaa taccttggcg tttacctgct tcaatacttt ccccaatttc tgctaccatt
     1981 tggtccgtat tttgtgattt accactccaa cctaaatcac cttttgctcc taaagcattt
     2041 ttaatatttt gggctttagt actactgccc gtattgccac taatgcctaa cccagctgcg
     2101 atggcagata taactgctga tgtattagta actccaaatt tttgggcaat cttcttgcct
     2161 tgatctataa cgtaagataa ttttctactt tcagctgcat ccaaacccat tccgtaacct
     2221 tcagaggcac ttctatccat accataattg gttgcaaagg caaggcggtt tgcattgcct
     2281 tgagatacgg actctcgaaa ggaagcttgc cattgttggg cagatgtttc tgctcggtct
     2341 gcagccttcg aatatgactc tgattggcta atctgtgatt gagaggaaac accaaaacta
     2401 ctattcctca tctgcgctct actatctcca gttgccatat tccgccatga tgttccggct
     2461 gcagattgag cagtcatcat ttgaggatcg gtatatacag ggtttaagct atgtttgttg
     2521 gcattgctat tgttgtagtt catattgccg gcagagtaat tccccgaccc tacttgggaa
     2581 cccgctgctt caccggttga tttggccgat gaagtcatgc ccatcatgcc gttcatcagg
     2641 ctgcgtccgc ccatggcaag agcacccgcg atgaccggaa tcagccacat tgcagcccca
     2701 gcagctgctt gtgcatcggc tgctgccatc aataacgtat cacctgttcc cggtgtaata
     2761 acgccgttgg cggcttccac gctaaacaga cgcttcgcat tcatagtcat aatcatattg
     2821 atgatactgg ctacgggtgc ccaaagctga atccatagca tactcatcat gtagaagctt
     2881 aataaccgga atgccatgat gccacccaac atggcgagaa tcattacaaa ggggaataaa
     2941 ccaatcagaa tggcctcagc agcagaacga atcaagggaa gattccaact tgccattgat
     3001 gagccggttt tttgggccgc aatatactga ttacgagctt gaacttgagc aaatgcctgc
     3061 tgtacggcag ccggatttcg gttgcgttgg ccattgataa tgcctgcttg acgaataacc
     3121 tcaaccatcg ccgcctgttc gatatttttc aatgtatcga agcgggcatt atccagcaga
     3181 tattggaatg agctattgcc taaggtaacc agggcattta cccggtttat tcctgccgaa
     3241 tttgtaccca aacctgcaaa aacacgatcg gcaatgtagc cgggcaagta atttttgcta
     3301 taaaaccggg tacggtcttt tagttctttt gccgcctcat tgcaggtcat ggtctttgat
     3361 ttgccgttat actgcaacat gttgcctctt cttgaggcga tctgcgatac attggtaaac
     3421 aaggcttggt tggtatgggt caatgtagcc agaatatcgg cactctgtgc cagatcctgc
     3481 acgttataga agtgatacag gttcatatca aagaatgaac actgatacat gaacagtgcc
     3541 cagtcttgag tcagttgcgc gtccgggatt cgtgcttcac ggatattttc catcaggcgc
     3601 gttccgaaca gcatgccggt gcgtgagtaa ttgaggtcgt ccggcaggtg gttaacctgc
     3661 tcggctttgc gggtaatact ttggccaaaa gaactgacaa agtagcccat ataagcaagg
     3721 ccgactggta cattgccgac agtatattga cggcctgtat atccataccc acctgattca
     3781 gtaatgagta cagtggtttt agggaccatc atacccatcc aacaaaataa cacgacaaac
     3841 aaataactga agttatcctt caaatccaat ttgaagactg caacagcaag agtgaccagg
     3901 aatccaaata aggcagcagc ctgcattgcg ccacccatca tcgtagatcc tgatattgtg
     3961 gcaacgccat tgaaaaccgc ccgtacagct tctccgttac cagggaaagt aaagtattcg
     4021 acagccatca tctctcctta gaaacggttc atcgaatcaa aagtcatgga gttgttggta
     4081 tccacaaagg ctgagcgcca ttgtgtttca aactgctcca tagccctcat catgctttct
     4141 tcgatctcga ttttcttcat tgagtcaatg cgctcctggt aaattctgct tcgcaattcg
     4201 ccaaggttgg caaacaattt tcgaaccgct tccccgctgg catcgtctgt cttgttatac
     4261 atacccaagg tcgagcgtac ctggcgttcc gattggtcca aaatattggc cataatatca
     4321 acggcaatcg catctaaaac cgctgttttt ttcaaatagc cagttccaag gaaagcatct
     4381 gatacagcca ttttcagtat gggcaattta ctgttattga caatggcagc aatctgcttg
     4441 cgcgtagata aattccaacc ttggttattc cttattgcag tcggaagcgt ttcaatgcgt
     4501 ttgtgaacca tgtaccgcaa agaaggtatt tctacatcat ttttctcagt gcagttctcc
     4561 attaatttct tagaaccgtc tttgcacgtc cacatatcca atacaacctt ctcccggatt
     4621 tctcccccgg cattgttttt tccgtttaaa agatcttgga tgctgaggat ttttggttcg
     4681 aaactttgaa tacctactgt gtatttgtcc ttatcccctt catgttgtgt taatggagtg
     4741 aacacaattg ttcccagcat gctgtaatag aagtccatgt cgatacccag tttcgagcgt
     4801 tgacccgtat agttcttcat ggcctcataa gtcaaattac cgccggtaaa gctaatcgta
     4861 tcttggggtt ttccggtgtt tcgcacctta tcggcttcat ggacaaggtt tttagcggcc
     4921 tgacagtacc aagcaccttc tgccggatca tttgacgaac ccgaatccac tgaagcggcg
     4981 cgacaatttg aactggccaa cgaacccagt ttccccattg cgccgccgac aatatcctga
     5041 ctcagtttgc aactgtttaa actgttttga ttaacataat tgaccaaatc acgaataccg
     5101 ccaattgcat cttggatcat cggatcaagg gccttaagtg ccaggtcaaa ggctacacct
     5161 gctgcgttat tgcccaggtt tcgcgtaaat tggatgaatt gttctttatt gacaaacgag
     5221 aaagagccgg caaagaaatc tataccgccg caaccggcac tgattgaagg taattgaata
     5281 ttcccaaggc tgatatccga caccggaaag cgaacgctca taccgcctcc cgtatatagg
     5341 ctgccgcttt gaccgtggaa cgcacctgct tcagtataat tggcttccgc tcccattgag
     5401 ttaaacatgt cagccatgtt cttctctatg ccggcactac tattgatcgg aagggccagc
     5461 gatgccgcca agcatagata ttttgcagag gaagcacaaa ctgtcggcac tttggccggc
     5521 ttcggaaatc gaaagatgtg aaatttcata accgctccat gaagcggtta tagcagggaa
     5581 atccttacta taaccaagat aagttacagt aaagacttga gattaaggga atcttatttt
     5641 acctgaaata tccgcgttgt caaccggtta atacatagaa atataaatat tgaaggcccc
     5701 gtatagggcc tttgatttga ttaatacaat gtcccgacag ggcgggaata cgctaagaaa
     5761 cgatcctcaa gcgtggtttg cgccatcacc ccaaatccaa tgggtgtaaa ttgcttggtt
     5821 tttgtatcca tgacgtacat tgcaggtaca gtagtaatat tcaacctttg ggcaataccg
     5881 ttgttcggca aagcacgcgg gaagtgatta atacctccgc catcgagact gaccggcata
     5941 accttaatac cgtattggtc ggcaaagttt ttcaaaatcg gcgccatggt atgacagtaa
     6001 gggcaatccg atttgaaaac aaacataatg ccgttctctc cggcaagttt tctaattgcc
     6061 ccggcctttt gttgctgacg gttatgatca tattgttgct gggccagttg gttgttcgga
     6121 cgaccgcgct ggctgtaatc caaatttggg ttttgccaaa ttaccctttg ccattgatcg
     6181 gcaaatgccg cagatttgtt catcacaacc tcttgaagtt caatatatcg cttcaaattt
     6241 tgaggtgtgg ggttcatgac ggcaatttta cgcgactcat ccaaatttct ttgcagcatt
     6301 tcaaattctg caagttcatt acgaaccgga gtattttgag gccggttttg tccggcttgt
     6361 ttgggctttt tagcatccgg ataccataag aaagcacggt tttcgttttt ccagatatct
     6421 tggtcatacc atcccatgcc atcatccgca gctcgcagat tggaggcgtg caggctaaat
     6481 atcagcatga cggccatccg cactcttttc attttttacc accgttcttt ctgttcgggc
     6541 acgccccgat atccataatt tcagacggaa aggtttgcga cagtgcgggc gaagtatgga
     6601 atgtcaagcg aaccctgttg catccgggtt tgtttttaat ttcttccatt ctgaccgcct
     6661 cgacaaaaat aggtatgtcc tgccggaatt tctgtttgac ggcagccgca gcacggccgc
     6721 tgaaataccc ttttgccgaa ccgtgacgca ttgccagcgc aagcaaggca tccggcttgc
     6781 cgtcaggaag cgggatggaa gtccttgcta gagcttctgt tttttccaaa gcgataccgc
     6841 cggtataagg aagggatgcg gcaagtgcca tacctgtaaa aaacataccc gatacaatac
     6901 ccgccgacgc agccaaccaa ttattgatat tcataatagt tcttcacttc caaatttttc
     6961 ccattaacgg atgcctccct gattttttgt gaaacctttt gcccctgctt ggccacgttg
     7021 tccatgttgg catagatttc ttcataaaac ccggacagat caagtttgga aaagtctaac
     7081 tgttccaatt cctgtgcggt aaatccttcg catttcgggt tctccggcgt accccaaccc
     7141 cgtttcaatt gctctttgcc gccgacattg actattttgg ctaatttgga aatgaaacag
     7201 cactggctct ctatcattgt tacacatgcc cccaatattt tgctgccgca ataagaaccg
     7261 acataatggc acaaatctgc tttacgcttc atggcaacta aaatttcact ttgcgggcac
     7321 ttcaaccact gttggatggc gattacggca atagcagccg caaatacggc cgggttgaag
     7381 ccgacaatta cccccatacc ctgttgtccc gcagcagaga aacccaacgc accaatctga
     7441 gatgttccgg taataccgcc ggccgcactc agggccgtac ccattgcctg tgctgcggag
     7501 ctgacaaaca tagtggtaaa cgtataatgg ctggcaacgc tggccataaa atacgttgtt
     7561 gccatagttg tccctgtctg taccgcgaag tcaacaaatt tgcccggatc tgcttttgtt
     7621 cgacagcagt ctccgccgaa tacgctgtct aaagcaccat ccagcttgat agagcattgc
     7681 ccggcttctc cattgaaaaa acgctgtttt tcgggatcat aaaaccgttg cgtctcacgg
     7741 gcgaattcca tcgtcgtcat agttttggca aaatcccgat cctgacaacc ggatacctct
     7801 ttggttgtct ctcctttttc ggtacattca aactgttttt ctctggcttg acaggtaccg
     7861 tccggcagct gatgcgtaca cttgtcccca atcagacggc attggtctga ggatgtataa
     7921 gcgctgcatg tttcggaatc gcttccgtcc gtacattgca cggtgcgcct ttctttccag
     7981 caatcacgtg taacgggtac gccgttgatg gtcttggtac tgccgggttc caaacactct
     8041 gttgcaactg ctttacattt ttttccttgt acctgacatg acgactcatc ccattgcgaa
     8101 ctaatcagat gggtatgctc catttcttcg actttggtcg gaagccttcc ttgatgtaac
     8161 gttctcaaat ccacatcgca agaatacttg tgcgtgtaac gcaaacactc cccctcattc
     8221 gactgctcca aacaggtttg tccctgtact tcacattttg ccgaagggtc ttttaacggt
     8281 gcgcaataat cggcagaatc cttctcaagg cactgatact cttctttgta ttcccaacag
     8341 tctttagtta cgtcgacccc gttgatgttt ttggtcgacg ggccatcaac acaaacggaa
     8401 gacgtccggg tacacgctgc actttccctg agtgcggcag cagacgctga agtgattaac
     8461 ataaagtggc agatcataac cagcaaagac gttatagccc tcattgtttt accctcttct
     8521 catactccga acaactgttt tcccatttat cgtgacaaac gggctcgcag tattggtgta
     8581 cattaaagaa gatagcattt tctccgccgc ctccgacaac ggtgcgggtc caaatggtat
     8641 ttttgccctg atgcagatac ggccgtatgt cgatgttcag gtttttcctc caggatttac
     8701 ctctttcagc gttacccaac cgagtacctt cctcattacg taccggtgca aaccttcttc
     8761 ctataggaaa tccatcttca tcttttgcag tataatctgc cgcaaacatc tgattgttat
     8821 agctgctgta cacaattttg ccgttgactt tgacaatcag ccaatcatcg tactcaaccc
     8881 ggctcaaagt aaacagcgtc agtttttccg gatttttcac attgagcgtc atctcccgat
     8941 catactgccc gtcacgccaa tagttgtcgc caatagtacc gaacatcagc ctgtgtgcgc
     9001 ttcccgaagc cgtcagcctg accgtcatat caccctgcag gctgcctgct tctatacccg
     9061 aacgggtaca gtttggtcct gatttctcgc ataccacctt taatttcttg ccgcattgat
     9121 actgctggtt tcgatatttg ctctccaggc aggaatagtt ccagccggga tcgacattga
     9181 ccttaagggc cgcctcgcac aagaacatct ctgcgccatg gtattcattg caacgtttga
     9241 cggtttttac cgcaggaacg gtaatggttt ccgtaggaca tgccgacgag ggaactgtgc
     9301 cgacgctgcc gtcttcattg acgggatatt tccatttgta tttttccagt gtttctgccg
     9361 gatcgcctgc aattttctgc gtgcctttaa ccaacttttc atttgaagat atggttacat
     9421 cgggccgctt ggtacggctt cctgcaatca ggtttacacc ttcgcattct tgacgaagat
     9481 agagatccgg attggcaggc agatttttac agtcgttgaa tttggcgtca ccaaactgag
     9541 tcggaagaga aactccgcca ccgtaatact gttcagccat tggtacgttg tctgtatatc
     9601 ttggggtata ttgggcaggc tgcatcatgt ttgttgcagt cccggccccc aattttccca
     9661 gttcgccgac gtaactaccg taatctgcag caaaggccgt ctgcatgagg ccaaatacag
     9721 ttagataaat ggcatgtctt ttcattttct gccttccaga acggctaatt tcgcatttgc
     9781 aatatcggaa agttttttat cttcagactc acggatcaag tgttccaaag ccgaatgcaa
     9841 ggacacatca ccctttacgg caacatgttc cgtacacatc cccaattctg aatttacagc
     9901 gcaaccgtct aaaccatctt cgctgtttcg ggcgattacc gtaacaggga cttcggtgat
     9961 gccgtaatgg tcgaagagtt cgggatgaat cagcatatcg ccttgcaaag ccgcaagttc
    10021 ttcggccgcc ttaaccgtgg ctttcagtga gtcgtcttta aacccgcgga taaccattac
    10081 cccgcctgcc tttgctgttt cacgggcaat gcgtttcaag ctggcttcgg gcattgaaaa
    10141 tgatacgaat accattaaat ccgctttttc cgcagcctgt cgggcttctt gtgaggcagt
    10201 gcggtatttt tgtgcgatag acatcggatc agccttgatt tttgcatttc cgtaatcacg
    10261 aacatcgggc acacggtgct tgataccttt caaatccgga atttcaggtt gctgccgatt
    10321 gtcacccaca tttgggtttt taactcgggt aactttaccc atggttgtac gcagacggtt
    10381 tcgcgccgcc tctacatccg catcggcgta agcactgcct actccggcca aaccgcccaa
    10441 aagaatgagg aagacgcctc cggctacgat tacattgcgt aaagacttgt ccatcactcc
    10501 atctcctgtc aattagtctt aaatttatat gcaccgcagc agtcgcgctt acggaaaagc
    10561 tggaaagcaa actgattacc tttgtatgga aatgctttgc cgctgtctct gactaatgtt
    10621 gaacgtccaa taggctcaca gcaacggcct ctgaattttc gggcggccac cggataggtc
    10681 atagccattt tatagttctg tttatccatt acgggattaa gtttgtaccc gcataaagca
    10741 tccctgcctg atgtcctcca agctactaag tccctgtgca tttttgtctg aattctagcc
    10801 gccatcaatt tagcggcctg cacttcggag aagtggccgg acatccatcc tgacaaaggg
    10861 taaatcgttc cgtggcaacc tgcacaccaa tacaatttat tgaatggaaa gccggcggcc
    10921 gctttaacac agtcagccgc acaggctgct tgtgcaacga cattggcaaa gagcgcagaa
    10981 tcaggggctt gataaacggc caatgacgga tatttccaag tgggatcaag ttccgtcatc
    11041 caaccgatat ccaaagtacc tttttccata caatcgttgt ccaataaggc ttccaaccaa
    11101 aatacgaccg gatttttata ccaatgaact tgataaaagg cattatgctg atcatgttgt
    11161 tcgttacgac cacgcttatg cgagccgcca aaaagatttt gaatcttatt ggatacgtct
    11221 gctttacctg ttgtttccgc cgtatctgct ttttgatttt taagtgaaat accgcctagg
    11281 ctaaccatac agaagggggt gcgtacgact tctaccaagt gtgtgggttc ccaaaatgat
    11341 accgtcacgc ctgaacggct cgggtgcgta cacgaacaaa accaattgtt tggagaagag
    11401 ccgttatcct cttgattatc catattcaga ttgattttgc cgccgatttt gattgggaac
    11461 atacagctcc aacaaatatc atttatcgga ttaagcatct tcccctggca ggttaattta
    11521 ttcggtatcg gagcagtagg aacagcttca gcggcgtgag atatagaaaa taatgggagt
    11581 agtaataacg gaatcagtaa tttcagcggt gtgacaaata ttttcacggt ttcatgacct
    11641 ctacacggag ccggccccct tcttgggaga ccaaacttgg aacttcattc agctgaaaac
    11701 ttttggacat ccgcccttct tggtcgtaat aaaccgcctg atccatttct actgataact
    11761 cataccaatt gcctgcagtc agaataggca tgaatgattc gccatattct gctgcaagtt
    11821 tcttggccag ttcgatttgg tcaggttccc ggccgtcaaa gaaaaacatt ttgaatggga
    11881 ttggggctgc ttccaaaggt ttgacaacag taccggcagg agcaataatt tgaccgtctt
    11941 ggtcataaat tgtttcggtt aaggtataac ttgggtcgaa gtatcgaaca gagcctttag
    12001 ttgccttcgc cagtccttcc aagggctgta gatttaaggc ggagcgattt attctatctt
    12061 ggacctcttt ctgcattgcc gccaattggc cgctgtcttg tttagctttc aaacgcgctt
    12121 caatttcgcg gattgcatgt ttttccgcta ttgggtatgt ggtgccgatt ttgtcgccaa
    12181 ccttctcgac agagggtgtc gaagcggcta cccatgagga aattccggtt gcaagtatta
    12241 ctagcgatgc gactgaagct atttttttct tgtgggacat actcactcct aaaaaatggg
    12301 gaccgcctta ccaataattt gccctgaatg gattaagccg gataattcat atcgactgtc
    12361 caagctgtct ttgtgccctg ttttaaccca catgaaccgt tgtggaattt ccccttcgaa
    12421 tgggttgatg gccagcttca tgccatccag ggagtattct tttgcagtac ctacctcttt
    12481 cccatctatg aaaaaagtac gattcgcctt cgttacatga tttccgggaa gtccggcaac
    12541 ctctttgacg acctgcgtac caatagggta aaaaccacct tgccatgcaa aggccacata
    12601 atcgccctgc ttcagatcac ttagcttgtt cgcatctttt ttgatgaagt aaagatggtg
    12661 aggcaaggaa cgggaaagat taaatgccaa agcataatgt tgattgaatg ccgacaagca
    12721 gaccgcaact gctaaaaaag gggttgcata tttctgacaa aactccctat aaagacgttt
    12781 catcactgct ttgttgcttt ctttttgtga tatttttatc attgcctcaa acattaacga
    12841 tccatttctt cttcagtaac cggggtgcct ttaccctctt cggccatcgg tttcggaatt
    12901 gtcttttcaa cttgttgatg taccggtgcc gccactacag ctgtggccgg gacacgctta
    12961 tccaactctt tcataatgtc agcagtaaca tcaaccacct ctgtgttccg tccgaccaat
    13021 gcagatttga caaagatgtt acagccgcac cgaattacct tttcttcgat aacctgttgc
    13081 aaggtattca tctttgcttg gtagttttgg aggatcgcgg ctcgttgccg gtcggaaatt
    13141 ccattctgca gtccttgatt cttcgcctgt tcccggtaag tcatggagat accggccaaa
    13201 tctacaatat ggaccggttt ctcatgggtt ttcatgaaat tccatacgcc taatgccaat
    13261 gcggcaatag aaattgcgcc gatcacaagg ctgcccaatg aaacctctac gccgttatcc
    13321 tccggattct tttccggatc ggtatttgcc gtgttttctt tcttttcttt tgcttcagtc
    13381 atcttattaa tatcctatcc tctgcctaat tagcgggttg cggcacggtc ccttaaaact
    13441 gcttcaatgg cctcggctgt attcaacccc tgatttctgt aatggttcac ggcttcaacg
    13501 tcttccgctt ttgagcttga ggcaagaacg ttgaatgggt ccgagtacaa cctacctact
    13561 ccataaccta tcggggtaga gacaaatatt tcactgtagt agtcagtttt gctcaaagtt
    13621 cgcagaatct gcataatccc gtcattgact tcaaaaggtt ctttgtcttg aatggttttg
    13681 agcgatttag ccccggtacg caattggaaa acaaagtcgg cattctctaa tgccgcctca
    13741 gccgccttag tcttgtagta gtcatttgca ccttgcgtca gagtgccgca tgcacctttg
    13801 tacttacgga cacggcggta gccttcctca ataaaacgag cggctgtacc cgtgtcgccc
    13861 aacaaagccc aagcctcgtc aatcatcata attttgtact ggcttcgtga taggtacatg
    13921 ccctgcataa tgcggtacat aatcagctgc atgatgatct gttgcagatc cggtttggtt
    13981 tttaactctt caagttccag tacgaccagg tcatttttaa actcgacatt caattttcca
    14041 ttgaagtaac tgccgtagat accttccgaa cagtatgtgt acaattggtc ggcaacggcc
    14101 accatagaca aatctcgttc accgggcttt cctccttcat tactgccggt aatcaaacac
    14161 ttgagacagc gttcccttac atgatcgacc gtgccttctt gtccgaattc gtaccatact
    14221 tcttcgatta tccgttgaag cttggcttcc tgctgttccg ttagggagtc tgacggagag
    14281 gccatcaatg agaagattag tttcaacatc tcaatatctt cgttgatgtc cgttaccatt
    14341 tggaatggcg ggaagcacag gcctgcttca taggtaaatt caatatactg cccgccaatc
    14401 atttggcaga atttttcata cgaacggcca acgtcgataa tccatacctg agtccccagc
    14461 cccatgtagt tgcgcacaat ttcattggca aaaaatgatt tgccggaacc ggaagcgcca
    14521 ataatggcaa agttatagtt acctgccggg ttggcaaata agtcgattcc catggcctgc
    14581 ccgcgttttc cgaacaaacc gatgacaggt cttcctaaac cgtgccactc gccgataacg
    14641 ggagacatac agacagcatt agtcatggtt ttggttgacc atcttttctt actgtccaaa
    14701 tatttctcaa cctctttgtc gatagccatt gggagggcag tcaaatatga ccccagttga
    14761 agatattggt cacgataaag accgaagccc tgagcctgcc aaatcgatac cgcctggctt
    14821 tcagccaagt taatgttttc gggacgctcc caaatgacaa cttggtgcaa caaactcacc
    14881 agaccgccgc cgcctttgcc gaacgcttct aggcatatgt cgtaatcacg tttgattttt
    14941 gctgcctccg gcataactgc ggccatcttg gattccgccg tttgtttggc gcgggccgct
    15001 ttcagtttta cggtattgct ggaagtgtca taatcacgct tgaacatcgc catggaaatc
    15061 agaaatggtg atgtgtaatt catgtttgcc tcaatgacat caccaatcaa tgcgcccata
    15121 ttggacagat gaaactcttc agggtagcga ttggcagaat agcattgagc aatcacgcaa
    15181 tcccccgcgg cacggttgcc aaaacgcagc cccgagccgc tatcaagaac cttgatagat
    15241 gtgcgggtct caatcatttg ttcactcaag aacttggttt catcccaaac aggatcgatt
    15301 tcatcttgtt gtccggtaaa catcctatta gggttgagta ggagcgttgt ccaagccaat
    15361 aaatcgtctg cattccaagt tcggccgtaa atctgcatgg agcggagtga atcgcgcatg
    15421 cgggcaagga gcgaaaccgc cttgctgact tccactgcat tatcaggtgg aatattcaaa
    15481 tttacgctga ggatacagcg cagatttcgt aaacgaatgg ccgtattttc taccaagacc
    15541 ttctgtgtac ccttattcca aaattcagta cgttttttgg ccaaagtttc gaaagtgctt
    15601 cgcatcggat cgtctatagg tcgtgatgct tgtacttgct catattcctt gaagtaattt
    15661 cttaaatcgg gggagccgaa aatttgaacc tgtatcggcg tcttttcagg aagcagcgca
    15721 tacaggctgc cccacttacc gatcaggtcg tcactgccgc ctaaaaatgg gttaagttcc
    15781 agaataaagc ccatgcccaa cggtttgtcg cctttaacgg acatcagcgt aaacaactct
    15841 gttttggtat cgtatgcacc gtaattgaga tagtcgctca aatggtcaaa gtcaggatcg
    15901 acagggttgt tgtgcaaggc ttccatcggt tgatcgacat catccgaacc ggccaaccaa
    15961 tttttaaatt ttgacaggac gcccattaca tacctctatc gaacggtacc gggaataccc
    16021 gatgtttcct gcagatgaag attcacgcca acatcgggga tgcggtcatt tgtgttgttt
    16081 gaattggttg gagaagtctc aaccgaactg cctgaagttt tggccccatc ccccaataag
    16141 cggatgggac gatattcatt tataatctgt tgcccgttgt gggcaattaa ccaatcaccc
    16201 tgattcagaa cgacatatgt gaaactttgg tcatttaaat cgccgtccgt atcaagatag
    16261 ggagcgaccc aaatgcgagc aatttcactt tgcgagcgaa cgggcgtacc ggaggaataa
    16321 gcttgcgtta acattaatgt gcgaacactt tgatcagtgt aaggttgtcc aacctgtctt
    16381 ttcataccgt cttttaaaac acctgcaaca gatgctttat agttactgct tatagattca
    16441 caataaggat cgtcactgtt ccgggcatta gggcatcgga atttttcact accgccgata
    16501 ccggacatgg ttaaggttga acatccgcta agaatcaata ttgccgcagt aatcgataag
    16561 gctttcaaac gcatcattct gctcccgctc catttaacca tttttccaat tcggcacgcg
    16621 gcatggcacc ggatgtacgc cgaccatctt tatgaatcat gcttggagta ccgcgaactt
    16681 taagctgctc tgcgagtgca atattttctg atacagggtt tttacagtta ccggccgccg
    16741 gttctttatc catcagcatg tagttattcc atgctttttc acgatcttta ctgcaccaga
    16801 tatgttctgc ttttgaaatt gcatcagggt ggagggattt aatcgggaac atgaaaacgt
    16861 atgcggtata gtctgtcatg ccggccaatg tttcttccaa acgacgacag aacgggcaat
    16921 ccgggtcgga aaaaagtgca aattctcgct tgcctttgcc gccattacct ttaacgattt
    16981 tgatggcgtt tttgaatggc agtcgggaat aactcttttg tgttaccgct gaacgggcca
    17041 tctcggtcaa gtctttctgc tcttgcatat cgtacaacgc gccaaaaatg aaatagcgac
    17101 cgttttcttg tacataggca atgttgtcac ccattgtcac ttcataaatt gaagccatgg
    17161 gggtagcctt tactgaagaa aacgtggtat tcggatacaa cttgtgcaga gtgcttccaa
    17221 cagttttgac atcggcaaag gccgtctgaa aagctgtgat tgctgcaata gtggccaaga
    17281 ttttcttttt catagatgga tcctatttgg tttcgccggt atcaatttcg ataccttgag
    17341 tcagtacgac atcgacttga cggcctgcat tgacttcaac taccgggaag accttatcgg
    17401 ccagttttat gtaatactca gcaagcctgt cagctgcacc accgatacca gatgcaatcc
    17461 ctgcacgata ttgatcactg ccccgaatag aggttgtgct gccaaatgcg gtattattcg
    17521 tcaccttgaa tgcttccgat acgcccttac ccataccgct aatcacgccg gacatcagtg
    17581 catttgccaa gagttggcct tgtttcgtta caagattacc tctcacgcct gttttgccgt
    17641 cttcaccggc aacgtaacct tttaccggca tgtcgatggc tcggccatcg gtacctacac
    17701 agctgagatt ctgcagtctg atgtaagcgc gttctgaaga tatatcgccg taagcctgcc
    17761 cggttacgaa acattctctg aaattgtatt taaaccggtt gggaagttgc gcaaaatccg
    17821 ttacgcgcat taaaacaggg tgggggctgg cattttgtgc ctcacccccg gtcggtgcgt
    17881 caaccccacc taacagtacg gatcggaaaa aagtaccgct aggtatatac gagtcacgcg
    17941 cccgaaactt gccttgcgag acgacaacct taccgccgtc ttcaactact tctatattca
    18001 tacgcggggt ttttgattta acccactccg cttcttttaa ttgcggggaa acccgtacat
    18061 tttcattcgg atttccggta tttaccgatc cggtcgttcc aggttgcagc tctgcttcac
    18121 tcatacttcc tgaaacgatt ttccgttcaa tgggcctatc tgccggaggc gccccgttcg
    18181 gcaatgtgcc tgccggaggg gccggaggaa gccggccgtt atccccgata ccgctaattg
    18241 caggcggttt gcctaatcca tcaggcccca gccccttact tgtatcagga ttactgctgt
    18301 tctctttttc ttttaactcc tgaaccatgg tttccaattc gctgattttt cgatttgcat
    18361 attcaatctt cgaactttct gtgttcatcc ataaggaatt gtccgtaatg cctgcttggg
    18421 ggcttgtaaa atcagttctg agaatctgcc gtttgttttg aacctgtgtg acttcggcag
    18481 atttcttttt attgccttca atgacataaa ttgcactgcc cacaatcgcc gccatgctgc
    18541 ccgcaaccat gcccgcgatg gctaactggc gttttcgcgc tgctgaaagt ttaaatttgt
    18601 ttactttcac cctcatctca acctcccgga cggctgataa taaatacgtc ggtaatttcc
    18661 ccgggttgta ggatctgttt ccgaaccgct accgccaaaa ctccttgtcg gtaaaattcc
    18721 tgctctctaa tttcaagttg tttagtacct aaattagtta atgtaaaaga aaggccttgc
    18781 atatcagcgg cggtgtaact tccgttttga ataaagaggg tgtttttcca taatgggacg
    18841 gtttgatatt catgactatg attgatgccc atatcgccgg tagtgccacg catcatagag
    18901 gccatcattt ttttgatggc ccgtacatat tctgtactat tcatcgttac aggcattgga
    18961 ccggtttgcg aagccaatac tgctgcttca cggcgattag ctccagccac atcgattacg
    19021 atgttgtcac cctgttttga tgtaggttta agaaccagca aataggtctt accttcttcg
    19081 cttataacga aaagatttgt cggattggag gtcaaactac ggacaaacag ttggcctgtt
    19141 gttgtatctt tctgaagctc aagatcaccc tccataaatt tccatgatga aattcgaccg
    19201 ccttcaatgg cgattctgct caagttccgt ttacttattg aaacaacgtg aggtgtattt
    19261 tctgttgccg gcactcgttg cgctgcaaaa ctagtcgcgg gtccggctgc caacaaggca
    19321 gaaaagacaa ttttttttaa cagtgaacac atatcaatta cctcgattac cgcctttaaa
    19381 ttctgttgcc gttttactga tttctacatc ctcggtttca acttcggatt tttcctgacc
    19441 tgcgggttct gctttagtta cacgcttttg aacttcttta atcgataata attgcagtgt
    19501 tccataatta tttttgaaag aaataagtag ctcgcggtct ttttgctcta ccagggcatt
    19561 gttgcgttta acctcaaaaa ccccacgaat ggaaactgta ttgtctgaaa tgtccgccgt
    19621 tgcttctgtc gcgctaaaag tggtgccgac attatttttc ttaacccata aggcatttaa
    19681 agacagctcc ctctctaggg catctttata atcaggtgaa acaatcttca ataattgact
    19741 attctgataa tcgactgttt taggggttac atttaagaga agataggcag catagggagc
    19801 catttcttca agatattctt tagaaaaagc gatgtttgat acagtcattg accgccggat
    19861 ttccggcggg gtcagcgtcg tttgaacaat cgtacctttg aagagcaggg tcgcggacaa
    19921 aaatagattg acagctatag acattcccaa aagtatccaa aaaaagacct ttatgccggt
    19981 tcttatcgcc aattcactca acatatcttc agtcttcaat ttagaaactc ccgtttattc
    20041 gtttcaggta tccgtttgtt tttttcatta atgggtaagt accagtaaag aacatgcaaa
    20101 aaaaaccatt gatgcttgcc agacttaagc ttgccccacc tccatgcaat gaaaaggcta
    20161 attgacatgg aaatgaaaaa gtgacgagag acaataccta taccgaagcc ggacataaat
    20221 aatataaaaa cgtccaagtc ccaaataaat gcctttttgg gatcgtctat cgataccggc
    20281 acatgataat ctctcatcac ttctcttatc tagtttgatt aaataatcaa ggtaaacata
    20341 cctttaatga tgcccgggcc taatgttgcg gccaagccaa cacccaaacc gacgatacat
    20401 ggcatgatgg tttgtttcat caggcctacc gccaaaccga tcaagaatgc agtcaagccc
    20461 aaagccatac ccagataacc ttcagaccag cctgttacca tgtcagccaa gcctttgaat
    20521 tcggcacctg ttgtagcggc cattgccaag ttagcataca atgccaatgc aaccgcacta
    20581 gcggacttta ctaatttatt gcgtttgata ttcattatga acctcattaa acagagctta
    20641 tatcgccaag cttttgcgtc cgatcattct ttaccaagtg attacaacgg ttctcgtatc
    20701 atcactattg cagcaggccg gggaacttgc tgttcttatt tttgcggata cattatgttt
    20761 cctcagccat ttacctatta ctgctgcccg ttgtttagcc aaacgtctat taattgcagt
    20821 atttcgacct ccgatactgt cggcccatcc gcgtaaataa accgtctgag tatttttcaa
    20881 tttaggcaga acacgtttga gcgttctcac gccctccgca ccgaggtgcg aactgcctaa
    20941 attgaaaaac accctgatct catctttttg aagattggtt tgtgtttgag cataaaccgg
    21001 ttgttccgcc aaaaccttgc gctcaaatcc cgaacactct tcattgcggc agaagacaaa
    21061 ttcgcccagc tgattttgtt ccactgtata agttgcgtct tgaggcagcc cggccacttc
    21121 ctgttttggg gcggcacagg ctgtcagaat cattgcggag gctacgaaga gcgtgttttt
    21181 aatcgtttcc aattttgata taccttattt gcgtaagtca ttcttgcgcg atagcacgcc
    21241 cggcctttaa gtttggtaca tgcggcgtta taagcaccaa ctgcttccca tgatttccca
    21301 tgttgccgat aactatttgc caatatccat gcaccaatat gtacatttgt gcatggattc
    21361 catacatccg cccctgttaa accaaatttc ttgtggagta cgggtagcca tgaacggttg
    21421 atacccatca agccaatatc agctgtatga ctttcaatat tcggcctgaa agcgctctcg
    21481 gttaatgcga tcgattgcaa catggcggga tcgatgccat atttgctgcc ggcttcctta
    21541 taacaaaaag caactgctct tgcagatgtg agtaccaaaa cacccggtac gacgaactgt
    21601 aaaagtatgg cccttaatct catgtcttta ctgtcgttgt atgtatgact aatttttatc
    21661 tcgattcgac aatttttgtt aaatcaaacg aaattaacta aattttttag aaatgtttct
    21721 ttaaagatag attatggtat aattaaaagt tacgcttata ttttatgaaa ttaccgattc
    21781 ataacatatt gatataaaaa ggaatacgat aagggtaaag gctcttatcc aaaagccaaa
    21841 aggccgtctg ctaaaaaaaa atcgcagccg gcctttttta aaagctgtaa cggtaaagca
    21901 acagccttaa tggttaaagg aagggttcaa gggaaaaagg gtaaaagtta aaggtaaaac
    21961 aaaggtctgc atgtaaaaat gcaggccttt gcctattaaa aggggcagca ttatacctta
    22021 taaatcttat tctgtgacaa ttctaattaa aataacattt tttattttga tttttgacat
    22081 ggcaactttt ttttattata tgaatggttt gacaaccctg tctttttaat caaaatgaat
    22141 gaaacagatc ttcccaagct tcttagtgtc ataaatcggt ttacggatat cgataaaaac
    22201 tggtcttgcg tgttgttgtt ctggatacag cgtgctagcg gttatctgga aatgatgacg
    22261 ctggatgacg atgaaaatac ggcaaccttc cttatcgaaa aactagagaa actactagag
    22321 ccactgccga tagatattga taacacgact tttgcagaag cacttgccga tgactttgaa
    22381 atcttatttc taatggccca atacggttct gaatactgga attcacttga agaaagcttt
    22441 gaggaatttt gtattagaca tactacgcta cctaatcaat tgattgctga tattccacat
    22501 gcaaaaaagg aaaaagtcac catgtggcta aaatctttgc ttaaatcatc atgaaaacaa
    22561 gccttctcac tattgcatcc tcactaatgc ttgtcagcgg ctcaatgcta tacctgataa
    22621 atacgagaaa aagtacagat tccgtgtcac ataatgatga ggttgcccat atcaatcaga
    22681 acatagtaaa tgaccggttt cgcattttaa acgcgcatga actgattcag gtgctcgact
    22741 taagccctca aatatccggt attaagatga acttagggtt atcggatgaa aactggagta
    22801 aggatgccct tccctttttg gaaaaatata tagcctttgt tcaaaggctt cccgccagtg
    22861 agtctcatca ccatgcaggt gatggtggtt tagtccgcca tacactcgat gtggctgcac
    22921 tggctcttgt ggcatctaca tcgcaatcat ggcccccaaa tgccaaaacc gaggaaatag
    22981 cgaagaaaac tgccgtttgg cgttatggaa tcatgtgtgc cgccctgctt cacgacgttg
    23041 gtaaaactgt gacgggattt caggttgaac tgtttgattc ggctatcagt ctcgaaaaat
    23101 tattatggtt gcctgataca ggctccatgg ctgaaagtgg caagctttat tatcgtgtcg
    23161 aatttcctga tgcaaagtct gcttacagta cacatgccga aatcgcctgg acattctttc
    23221 aagctttggt tccttcacat gtcagacaat ggcttgccac aacggatccg aatttaatga
    23281 ttactttgag aaattatctg agtgggaaaa aggatggcag tcctcttgag cagttaataa
    23341 aaaatgcgga tatgacctca gtttcaaggg atttacgaag cggcagccgt caacgcttct
    23401 caaccgccaa gcgtaaacca ttcattgaaa ccattatgga aacattgaag gagatgttat
    23461 ccgatagagg agtccatttc agcatcgcaa caactgccgg tggtgacctg ttccgtaagg
    23521 gtgatcgcat ctatttgatg tcaaaaaacg tacctgacta catccggcag tatttgagga
    23581 aaaatcaaca tcctgctgca ggctcatttc cggctgacaa ccaaaggata tttgacacat
    23641 tattcgagta ccgtgccgtc attccgccgg aaaacgatcc tcacagggca attaaccata
    23701 tagaggttga gtttacccgt atggacggtg aaaaggtccg taatatcttc agtgtacttt
    23761 gtttcaatgc caaaaccctg tatccggacg gagattatcc gactgagttc ttaggtaatc
    23821 tttcagaagt ttccgccaag aaggaaatac cggatgttgt agtatcagag gataaagtgt
    23881 caactcagat acaaggtgcc gaagataacc cagtcactat gaccaaggac aaaatggact
    23941 tcctgtctat ccaaccggtg acagcagaac aacctcccct acctgaaaaa aatcaaactg
    24001 agacagctaa ctcggctttt gacaacagta tgaatactgc tgctccagcg gaaaataaat
    24061 ctagcgagtc agagaaagct aaagaaccgg gttacggcac aattgataat ttaattgaaa
    24121 actttaacct gatagaagct gattcagaac aaacagaatc aaccaaatca gaagccgaga
    24181 cagcagaaaa tgccgttaac ctggttcaag aaaatataag agtagaagtt aaaactgaga
    24241 cagtggacaa acctcgcgct gaggttaaag aaactgagac agataaagca ccaaaaacaa
    24301 ttaaatcagg aaccaaagca agccgtaaga aacttgccgg ccttttcgca gacaatcgta
    24361 aacctattgg aaaaacagcc gaacccgatg cacagattaa aacaactgag acagtaactc
    24421 atcaaataga ttctgaagca gtttcattgc ctgaatctga atcagcacaa aacggtatgc
    24481 gtgaactgga gcagctaaga ctaagagaac ctacatctag tccacgcccg gttcaagtca
    24541 tggatgactc cgaaggttta gatagccttg caagcggggt aaaaaatatg gccgaactcg
    24601 aagccatagt ttctgaacgt aatttgaaaa agcaatcaga aacagagtcg accgaaatag
    24661 tcgaggattc agtcaaagcc aaacatatgg aggccagaga ccgtggaatg cagtttttac
    24721 gatggctggc agatggatta ggagacggca gcatagcagt caatagaagt ggtgcaacag
    24781 tccactttat tgaacagggg atgcttttgg ttacgccagc cgtatttcga gattacgccg
    24841 gcggcgtgtt caataaatct gatacggaaa gtctaggccc tcttacacaa aaaggctttg
    24901 aggctttgag gttgcatgca agaacgaaaa aaagctcatt gcatagggtt ctgactacaa
    24961 acggtagcaa tcgcagactc ttctattgct accttatccc tgaacataat atcaaacata
    25021 tcatacagcc aggttcaaga cctcagaata ataccgatat taaattagat gaatccgact
    25081 tattagtaat ggaacaaaaa tgagtgccca cttccctgaa aacgtttatc gatcacccta
    25141 tgaatggatc agtgtagccg catactccac cacagcggtt tcagtagcac atttgcccgt
    25201 acctccgaca attgcttatg gcagtttagc tattttttcg ttggtttcag tcaaacgtgc
    25261 gttatcagca ttacgcatta tgcgtttccg taaaaatatt cggaagttgc cctattatgc
    25321 ccttactgcc gatgaaattc cttcttctaa taaagctctt tttcttggga aaggttttag
    25381 atggacacag acacataccc aacgtctttt tatggttcgc cagcctgaga acgacggact
    25441 tcgtaacccc tcaaaactct ataaggcagc gcgaaaacat gagattgaga caatgggtac
    25501 agactggatt tcccgcataa ctcggaaatc ggcatggtgg aatccggttg ctcccttacc
    25561 tccggtcgga ggttctcctg aattacatgg cattgaaatt gaggaagatg atatttggga
    25621 agatttgggg gagcgtgttg gccacaaggt tgtattgggc acaacccgcg ttggtaaaac
    25681 ccgattatgc gaaaccatga ttgttcagga tattcggcgg ggggatgtta cgatcgcatt
    25741 tgatccaaag ggtgacgctg acctgatgac tagaatgtat gtcgaatcgc aaaaatcaaa
    25801 caggccgttt tatatgttcc atttgggttt ccccgagtac agttgccggt acaaccctat
    25861 cggggattat ggccgtatta ccgaggtggc gacaaggatt gcaaaccaac tgccttctga
    25921 aggtcagtct gctgctttcc gagattttgt atggcgtttc gtcaacgttt taacgaaaac
    25981 catggaaggt ttatccatta aaccaagcta tgaaaatatc tataagtctg ctgcaaatgt
    26041 agatgaactt gcaggggatt actttaaaaa gatattagat aaacaccacc ctggttggga
    26101 gaaagacttc gacaattttg atatgcccga gtctgaagca aaaagcgcag tcaaaactgg
    26161 gcgcaacttg gacacaatga agctcggttc tttcttgaag gccaaaaagc ataacgaacc
    26221 tcttattgac gcattgggtg gtattttgtc caatgacaga tcctattttg agaagctagt
    26281 cagctccctg tatccattgc tggaaaaact gacgactggc gaagttgcca agcttatcag
    26341 cccggataca gaggatttgt cagatccaag aatcattatg gactggcgca aagtcatgga
    26401 atcgaatgcc gtcgtctata ttggattgga ttccctatct gatgccgaag ttgcagcagc
    26461 cgttggtaat gcgatgtttg ctgacttaac ctctcttgcg ggtcagatct ataaattcgg
    26521 ccatggggtt ggacagagtg gaaaaaccca gaaaagaaaa gtctcaattc atgcagacga
    26581 attcaatgaa ttaatcggtg acgaattcat tccgttattg aataaggccg gtggtgccgg
    26641 ctaccaagtg acggtttata cacaaacctg gaaagacgta gaagccaaaa ttggttcccc
    26701 tgctaaagca ggtcaaattg gcggcaactt gaatacgatg attatgctta gggtgaaaac
    26761 tgtcgaaaca gcagagatgc tgacgaatca gttgccggaa gtcaaaatta tgaccagcac
    26821 tctagtctca agggctggtg atgttgcctt ccctgatagt cacgaagatt ttagcagtgg
    26881 aaacgaagac aggattggtg ctgagacagt tcctatgctg acacccgccg atttaaccca
    26941 attacccaaa ggtcaagcat tcgcgcttat tgaaggtggc cagctatata aaattcgact
    27001 ccctctaccc aaaaaggaca agcaggagga aatccctgcg catattggag aaatggcggc
    27061 tgatatgcgc gaagtttaca gactaagccg gccttcggct gaagaaaacg catttacgga
    27121 gggaagtagt tatgccgtct aatacaagcg catggtacat gaattcattg cctttaaagt
    27181 tcttatttct cccgtttaaa gctgcttata ccttattctt aattgtgctg cttatctttg
    27241 gtagttcggt gttgacacaa aactattttg gtaccggtaa tctcttggaa gatgaaatca
    27301 aatggtctga gtctgttggc catgatgcgt ttccaggaaa agaggttgac gatactataa
    27361 atctgcaaat tgccagactt agttacaagg ggctgaaaac cgtatttttt gacctgacat
    27421 tagtcaatga atcactgaaa gccaagtcag aagccgattt tggttacaaa tttaaaaatt
    27481 atttagtccc ccgtatgaaa tatctacagc aattcgatac tacgttgaag gttatttcct
    27541 tacgtttggg aaatttggca ttaatcgtgg gtatctcagt aatctttgca atcccggttt
    27601 tgatagatgg gttagtgatg agagctatca ggcaggaaaa tgcctcaagg gagagcgccg
    27661 gtatttatca ccgtgcaaaa tattggagaa cagggattat ttggttgggt tgcatgattt
    27721 atatgtgtgt gccaatatca ataccggcct acttgctcta tttacctctg gtagcagcaa
    27781 tttggatgat tttcatgcaa gctaaatatc ttaagaaata tctctaggct tccagtggaa
    27841 tctaaatagt gcttttttag ttcattaaac gggggcttaa tgctttaact gacggtacag
    27901 aaatctcgaa aagcattttg caaaatactt aaaataagag tatagtaaaa atgaaatatt
    27961 tcaagtttaa ctaatagtta acaatctaga gtaaacaatc gtaatcgtta aatctgggta
    28021 tagattgcat aatatacatt atgcgaacta ctggaaacag ctgtatgtgt tgcgttgacc
    28081 ttcaatacga tatacaggca gcaggcacac aggatatgcc gcccatactt ttttaatcca
    28141 aaacattata tcaacgggcg caaacagtaa atcgcccaac ttatcccaaa aattctcctg
    28201 ctttttcatc atttcttctg tcaaatcctt ggaacggtcg atttgaaaac acggccttac
    28261 atacgtccca attctctttg cagtgcttgg atattttgct gtctgtccaa aacattgctt
    28321 tgcaatgcgt tgattttttg atggttgatg ttgccgcctt ttgccagacg tgcttgtgat
    28381 aacatttttt gggcttcagt cagggctttg cgttcattgc ttaattctgc ttcgagaatg
    28441 gagcgtctgc tgttatttac aggtgcttgt tgaggcggcg tattggattt tgccggcttg
    28501 gatactgttt tgaccggggc tttatatttg acagcctgtc cgccgtttga cggtgatggt
    28561 gccggttcgg gagtttgggg cgggatatag cgttcgctgc tgtagttgcc gattgggggc
    28621 aaatcggttg agtggcagct tttagacggc ttggtggtgt aaacggtttc tccgttgatt
    28681 gtgcaggtat agattttggc cgcattcgca cccaatgggc ttgaaatcag ggaaaggttg
    28741 attaggatta aggggagttt tgatttcata atgtgtttga tattcggata ataatttgat
    28801 tttttgagga ttttttgtaa ggttttcttg gcgttttatg ggcttttctt ttcggttgtt
    28861 tatttttgat tttccttcgg ggctttggca gcctgtctgt tctttttccc ggcatgtttt
    28921 ttgtttttct ttttgtttcc tgtgtgtatt ttggcttctt gactaagttt tttaattttc
    28981 aggcggtatc cgcctccctg atggctgctg attttaggta aatcagcatc ggcacacgat
    29041 cctgacgggt ctgatgtata tacgcttttg ccatttgagt tgcaatggta tatggaggct
    29101 tgcgccgccg cgccggaaag ggacaggaaa cccaaggcgg caaaaagtct gatgtttata
    29161 ccggtaaagt aaaaaggcgg tggtaacgtc cgaattatac cgttattggc aggcaaagat
    29221 aagcaccctg cccgcgcttc tttatcgctc ggcaaactgt ttctcgattt cagtttcaat
    29281 ccgttccaat tcttcttgac aagccagcca agtttcttcg atttgggcaa gctgtgtttt
    29341 gacttttgcc agctcggata aggtattttg caatttttct ttgtttgctt caaggtaagc
    29401 gtcttcttgt gttaaaaatg cttcgcatcc cgtctgaatt tcagaaagct gcaccatttc
    29461 tttttcggct ttgtcgattt tctgttgtat cggcttactg cgtcgggctt tttcttgacg
    29521 gatttgcgct tcgatacgct tggtatcctt gcggttttgg ctttgagtgg aagcggctgg
    29581 cgcggttgcg gcgttttcct gtgccaagcg ccattggcgg taatcgttca aatcgccgtc
    29641 aaagtttttc agacgacctt tgtctatcaa gaggaaactg tcggtcgtgg cttcaagcag
    29701 gctgcggtcg tgcgatacga cgattaatgc accttggaaa ctttgcaacg cgagcgttaa
    29761 ggcatgacgc atatccaaat ccagatggtt ggtcggttcg tcaagcagca gcaggttcgg
    29821 cttttgccag ataatcatgg caagggcaag gcgggctttt tctccgccgg aaaaaggttc
    29881 ggttttctgc aacgccatat cgccgacaaa atcgaagccg ccgaggaaat ttcggatttc
    29941 ttgttcgcgt acttcgggag aaagctgctg tatatgccaa acagggcttt ggtcggcgcg
    30001 gatggtgtca agctggtgtt gggcgaaata gccgatatta agtttttcgg aatggacgat
    30061 gctgccggag agtaaatcga tttttcctgc caaagctttg ataaacgtag atttaccgct
    30121 gccgttgaca cccaatagcc cgtagcgtgc gccgctttcc agcgacaggg taatgtcatg
    30181 caggacggtt ttaccttcgt aacccaaatc agcgtgctcc agcttcagca aaggattggg
    30241 cagatggtcg ggattgtaaa actcaaagga aaactcgctg tccagatgtg cgggagcgat
    30301 gcgttcgagc ttcgccaacg ccttcatgcg gctttgagct tgaacggctt tagtggcttt
    30361 ggctttgaag cggtcgataa aggattgcag atgtttgatt tgcgcctgct gtttgacata
    30421 tgcggcttgt tgttgcgcca gacgctgcgc gcgttcggtt tggtaaaaat cgtaattgcc
    30481 gctgtattgc gtgagctttt gctgcgacaa ttcaatggtt tgggtggtgg ccgcattgag
    30541 gaaatcacgg tcgtgggaaa tgatgatttg cgtgcagggt aaagaggcaa ggtggttttc
    30601 caaccacaag acagtttcca aatccaaatg gttggtcggt tcgtcaagca agagcaaatc
    30661 agcgcggcaa atcagggctt gcgcaagatt caggcgcata cgccagccgc cggaaaagga
    30721 tttgacggga cggctgtgtt cttcttgcga aaaacccagc ccgttcaaca attttgccgc
    30781 gcgggccggc gcggtatagg cgtcgatttc ttccaatttg gcatgatatt ccgcctgctt
    30841 catgccgtca ttttgcactt ccgcctgcct caatgccgtc tgaaaagcct gcaactcggc
    30901 atcgccctgc aaaacgtaat ccaaagcgga aatatccaaa tcgggcgttt cttgggaaac
    30961 ggaagcgagc cgccagtttt tcggaatcga gatatcgccg ccgtcctgag tgatttcgcc
    31021 cttgattaag gcaaacaggc tggatttgcc cgtcccgttc ttaccgatca aaccgacgcg
    31081 ctgaccggga ttgacggcag cggaagcctt gtcgagcagg actttcaaac cgcgttgcag
    31141 ggtgaggttt ttgatttcaa tcataacgga aacatcgtcg ggcgggaaaa gcccgtattt
    31201 tacctgaaag tcagtgccga tgccgtctga aacgggaaat ttacggctga agccaagccc
    31261 aagccctgcg cccttccgag tgcaggaaaa ccaatgtcct gaatgccgaa tcggtattca
    31321 tgcattccac gctgattccg attcgggaaa aatccgccat gattttggga tggataaact
    31381 cctgagccgc gcccgtcccg ataatcaata tttccggata gtcaacaggt ttgatgtcgg
    31441 acaacaggtt ttccggagtc aggtcggaca aggttcggca ttgcggcagg cagaccgaat
    31501 ccttatgtac aagcacgggt ttatggaaac tttgccccgc cagccggatt ccgcccgcgc
    31561 cgcattcata ttctgcaaac tgtccgtcta tcggattttc ttcaaacaac attttttacc
    31621 ccgttgctgc atcatctgca ccgaaagggg tgcaaaatca ggcgaattaa tgtaggattg
    31681 gcagatttca tctgacgcgc ctgccgattt cagacggcat ttgattcaaa gtgcggcaca
    31741 attataccgg cagcggatat tttcgtcttt caatatttac atttcagtcg gcttacaagg
    31801 agacacaatg aagccagtaa acatcggtct tttaggctta ggtacggtcg gcggcggtgc
    31861 ggctgccgtg ttgcgggaca acgcagagga aatttcccgc cgcttggggc gcgaaatccg
    31921 tatttctgcc atgtgcgact taagcgaaga aaaagcccgc caaatctgcc cgtccgccgc
    31981 ctttgtcaaa gatccgttcg aactggtcgc gcgtaaagac gtcgatgtcg tcgtcgaatt
    32041 gttcggcggc acgggcattg ccaaagaagc ggtgttgaaa gccattgaaa acggcaaaca
    32101 catcgttacc gccaacaaaa aactgctcgc cgaatacggc aacgaaatct tcccgctggc
    32161 ggaaaaacaa aacgtcatcg tccaatttga agcggcagta gcgggcggta tcccaatcat
    32221 caaagccctg cgcgaaggtt tggcggcaaa caggattaaa tccatcgccg gcattattaa
    32281 cggcaccagc aacttcatcc tctccgaaat gcgcgaaaaa ggcagcgcgt ttgccgacgt
    32341 attgaaagaa gcgcaggcat tgggttatgc cgaagccgac ccgaccttcg acatcgaagg
    32401 caacgacgca ggccataaaa tcaccatcat gagcgcgctg gcattcggta cgccgatgaa
    32461 cttctccgcc tgctatctcg aaggcatcag caaactcgac agccgcgaca tcaaatacgc
    32521 cgaagaactc ggctaccgca tcaaactgtt gggcatcacc cgcaaaaccg gcaaaggcat
    32581 cgaattgcgc gtccatccga ccctgattcc cgaaagccgc ctcttggcaa acgtcgacgg
    32641 cgtgatgaac gccgtgcgcg tcaacgccaa tatggtcggc gaaaccttat attacggcgc
    32701 gggcgcgggc gcattgccga ccgcttccgc cgtggttgcc gatatcatcg acatcgcccg
    32761 cctggttgaa gccgacaccg cccaccgcgt accgcatctg gcgttccaac ccgcccaagt
    32821 ccaagcgcaa accatcctgc ctatggacga aattaccagc agctactacc tgcgcgtcca
    32881 agccaaagac gaaccgggca cgctggggca aatcgccgcg ctgttggcac aagaaaacgt
    32941 gtccatcgaa gccttaatcc aaaaaggcgt tatcgatcag accactgccg aaatcgtgat
    33001 tctgacccac agcacggtcg aaaaacacat caagtcggca atcgcagcca tcgaagcact
    33061 ggattgtgtg gaaaaaccga ttaccatgat ccgcatggaa agcctgcatg actgagccga
    33121 aacacgaaac gccgacggaa gagcaggttg ccgcgcgcaa aaaagcaaaa gccaaaatcc
    33181 gcaccatccg catttgggcg tgggtcattt tggcgttgct cgcttcaacc gccctgctct
    33241 cccaatgcgc gatgtccaaa ccgcaggcaa aacagaaaat tgtcgagtct tgcatgaaaa
    33301 atattccgtt tgctgaaaaa tggcagaacg atttgaaagc gcgcggcttg gatgcggaca
    33361 atacccgtct cgccgtcgac tactgcaaat gtatgtggga gcagcctttg gacggattga
    33421 gcgagaaaca gatcagctcc ttcggcaaac tcggtgcaca agaacagctt gacctgctcg
    33481 gcggcgcaaa cgcgtttgaa actcgagaca aacaatgtgt cgcggatttg aaagccgatt
    33541 gaacctcagg tctggaatgc cgtctgaacg cggggaaaag cgtttcagat ggcatttttc
    33601 catccgggtt tgacgggcag cccttcatcc gaatccgacg ggcaaacccc tatcctgatt
    33661 cggcggggaa aacaaaaagc cgattcttca aggaatcggc ttttgctgaa aaacagttta
    33721 cagtgcgtct ttcaatgctt tacctgcacg gaatttaagc gttttggcgg cggcaatggt
    33781 cagaggctcg ccggttttgg ggttgcggcc ttggcgttcc gcacgttcgc cgacgtagaa
    33841 agtaccgaaa ccgaccagag taacggtgtc gccttgtttc agggcgttgg ttactgcgtt
    33901 ggtagtggca tccaaagctt tttgtgcggc ggctttggaa atatcggctt cttgagcaat
    33961 cgcttcgatc aattcagact tgttcacaat cagtcccttc ctgtcttaaa aaatgatgaa
    34021 atgcccgaat actcggggtt tgtactgctt gagcaacttt cgctttatag caattctgaa
    34081 attgccgtgt caagcaaaaa atacggaatc gccctatttt acaggctttc aggacgaaac
    34141 cgcattttta caacacattt cctgcgtttc aatgtttgat tgccctgctg cggggtttcg
    34201 gttttgaagc ggattccgcc gccgcttccg cggcagaagg ttctgcccaa gactcaggcc
    34261 ggctttccaa acccaaagcc aatacctcgt ctatccattt gaccggatgg atggttaatc
    34321 cggttttcac gttttcaggg atttcttcca agtctttgac attgtctttc ggaatcagga
    34381 cgtgtttgat gccgccgcga agcgcagcca acagtttttc ctttagaccg ccgatcggca
    34441 ggacttcgcc gcgcagggta atttcgcccg tcatggcaac atcggcacgc accggaattt
    34501 tggtaaaggc ggacaccatc gccaaagtca tagcaatgcc tgcgctcgga ccgtctttcg
    34561 gcgttgcacc ttcgggaaca tggacatgaa tgtctttttt ctcgtaaaaa tcaggagcca
    34621 aacctactga ttccgcacgg gaacggacaa cagaccatgc agcggataca gattccttca
    34681 tcacatcgcc caactggccg gtacactgga tcatgccttt acccggtaat gctgcggctt
    34741 cgacagtcag caattcgccg ccgacttccg tccaagccaa accggtaacc tgtccgatgc
    34801 ggttttcact ttcggcaacg ccgtaatcga agcggcgcac gcccaagtag tcatgtaggt
    34861 ttttctcatt gactttaacc gccctaggct tggctttgct ggttttcttg gtttcagaca
    34921 acctcttctt atcttcgttc aaggtaatct gcatcaccac cttacggcag attttggcaa
    34981 tttcgcggtc gagcgaacgc acgcccgcct ctcgggtgta ataacggata atatcgcgta
    35041 ccgcgctttc ttcgaccacc aattcgcctt ctttcacgcc gttgcgtttc atttgcttcg
    35101 gtacgagata ctgcatcgcg atattgattt tctcgtcttc ggtatagccg gacagacgga
    35161 tgatttccat acggtcaagc agcggggtcg ggatattcag gctgtttgaa gtcgcaataa
    35221 acatcacgtc gctcaaatcg taatccactt cggcataatg atcggcgaac ttgttgtttt
    35281 gttcgtgatc gagcacttca agcaacgcgc tggcgggatc gccccgaaag tcgctgccca
    35341 atttgtcgat ttcgtcgagc aggaacaacg ggtttcttac gcctgctttt gccatattct
    35401 gcaaaatctt gccgggcata gagccgatat aggtacggcg gtgcccgcgg atttcgcttt
    35461 cgtcgcgcac gccgcccaag gccatacgga catatttccg ccccgttgct ttggcgatgg
    35521 attcgcccaa agaggttttg cccacgcccg gagggccgac caaacacaga atcggacctt
    35581 tgagcttgtc catacgtttt tggacggcga ggtattccaa aatccgttct ttgacttttt
    35641 ccaggccgta gtggtcggca tccagcacca gtccggcttt ggcgatgtct ttgctgacgc
    35701 gggatttttt cttccacggc agtccgagca gggtgtcgat gtagttgcgt acgacggtgg
    35761 attcggcaga catcggcggc atcattttga gttttttcag ttcggacagg catttttctt
    35821 ccgcttcttt ggtcataccc gcctttttga tgcctgcctc caaggcatcc agttcgccgt
    35881 tttcgtcttc ttcgcccaat tctttgtgta tcgctttaat ctgttcgttc agataatatt
    35941 cgcgttggga tttttccatt tggcgtttga cgcgtccgcg tatgcgtttt tcggcctgca
    36001 taatgtcgag ttcggattcc agctttgcca gcaggaattc catccgtttg ccgatttcgg
    36061 gaatctccaa aatctgttgg cgttgcgcca gtttcaactg caaatgcgct gcgaccgtat
    36121 cggttagccg gctgttttcg gcaatgccgt tgatgctgcc gataatttcg gcagggattt
    36181 ttttattgag tttggcgtat tgttcaaact gcgccaacag ggtgcggcgc acggcttcga
    36241 ggtcggtatt gccgcccgtg tcttcttcca cgaccgcctc tatatgagaa acgaacagcc
    36301 cgcccgtgtc ttcgatggtc aaaacacgtc cgcgatacag cccttcgacc agcactttta
    36361 ccgtgccgtc gggcagtttc aacacttgca ggacttgtgc gacggtaccg gtctgataca
    36421 ggtcggcggc aaccggttct tctaccgccg catcggtttg cgccaacagg aaaaccggtt
    36481 cctcgcgggt aatggcgttt tccagtgcgg cgatggattt cggcctgccg acgaacagcg
    36541 gcagaaccat atgcgggtaa acgacgacat cccgtaaagg aagggtcgcc aaagcagcat
    36601 attcctcaaa atgcttttct ttttgtgtca tggatactct cttgtgtctg acaggatacg
    36661 gattgccgcg taaattgggg ttgaaagtat tatttcaagc atacgcggtt tatttatgga
    36721 gtttgatgcc atgccgtctg aaacattccg gcttcagacg gcatgggctt ggaaagacaa
    36781 ggcgggaaca aaaaaacagt tctgtgttgc cgctccttgc tgtaccatcc gtatggtttg
    36841 cggttctgcc gccctatttt caaaacatga cgcaggtatc ccatgtcttc ttttattttt
    36901 cccgataacg ggttcgaatg gcgcaacgaa agtctgcaaa agccgcccaa aggttggtat
    36961 tatgtccgcg aaagcggtgc agacggcatt ttgaaggcta cctatcaaaa tattgcaact
    37021 gtctggcagg gcgatttcca caatgccaaa caggtgcttt cggcaatgaa gaagaaggtc
    37081 cgcaaacctg ccgccgtccg ttccgatgcg gatatcgccg cccttttcca cgcccaccgt
    37141 atgaagcagg cgcaacagag ccgtattctg aatatgcttg ccgttgaaat ccgccccggt
    37201 ttcgtgttgg acaacaaacg cgcgcccgat atacgcgccg ctttgctcga cgtgtacgga
    37261 gaggcggacg gcaaaccgtt ttttctgccg ctcaatctgc tgctggggtt tatgggtgtg
    37321 catgagtggc ataag
//
LOCUS       DAXDZG010000011        37022 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXDZG010000011 DAXDZG010000000
VERSION     DAXDZG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37022
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.011"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            218..838
                     /locus_tag="ACFB78_000471"
     CDS             218..838
                     /locus_tag="ACFB78_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC1268539.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            861..2312
                     /locus_tag="ACFB78_000472"
     CDS             861..2312
                     /locus_tag="ACFB78_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFC1268540.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTPFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLPPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            2773..3129
                     /locus_tag="ACFB78_000473"
     CDS             2773..3129
                     /locus_tag="ACFB78_000473"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC1268541.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            3120..3602
                     /locus_tag="ACFB78_000474"
     CDS             3120..3602
                     /locus_tag="ACFB78_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC1268542.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            3615..4208
                     /locus_tag="ACFB78_000475"
     CDS             3615..4208
                     /locus_tag="ACFB78_000475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC1268543.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB78_000476"
     CDS             4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB78_000476"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC1268544.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB78_000477"
     CDS             5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB78_000477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC1268545.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB78_000478"
     CDS             6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB78_000478"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC1268546.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            7618..7917
                     /locus_tag="ACFB78_000479"
     CDS             7618..7917
                     /locus_tag="ACFB78_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268547.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            8331..10592
                     /gene="nuoG"
                     /locus_tag="ACFB78_000480"
     CDS             8331..10592
                     /gene="nuoG"
                     /locus_tag="ACFB78_000480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC1268548.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASNRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            10595..11671
                     /gene="nuoH"
                     /locus_tag="ACFB78_000481"
     CDS             10595..11671
                     /gene="nuoH"
                     /locus_tag="ACFB78_000481"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC1268549.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGLRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            11752..12231
                     /gene="nuoI"
                     /locus_tag="ACFB78_000482"
     CDS             11752..12231
                     /gene="nuoI"
                     /locus_tag="ACFB78_000482"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC1268550.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            12243..12914
                     /locus_tag="ACFB78_000483"
     CDS             12243..12914
                     /locus_tag="ACFB78_000483"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC1268551.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            12911..13216
                     /gene="nuoK"
                     /locus_tag="ACFB78_000484"
     CDS             12911..13216
                     /gene="nuoK"
                     /locus_tag="ACFB78_000484"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC1268552.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            13219..15243
                     /gene="nuoL"
                     /locus_tag="ACFB78_000485"
     CDS             13219..15243
                     /gene="nuoL"
                     /locus_tag="ACFB78_000485"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC1268553.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRTKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15386..15889
                     /locus_tag="ACFB78_000486"
     CDS             15386..15889
                     /locus_tag="ACFB78_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268554.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMANINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKQTSKLSRWEVLKGVQANIWGICFLFFNLAI
                     VHSRLNP"
     gene            15892..16554
                     /locus_tag="ACFB78_000487"
     CDS             15892..16554
                     /locus_tag="ACFB78_000487"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC1268555.1"
                     /translation="MENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRI
                     APDFLEIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVR
                     GVVLIDEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGAYRLVRSK
                     EDGVVYGKMIDYPSNASFVDLLNEAFGVDLNKKKIERAQERNQEQAKKPYWNWYVRNW
                     AD"
     gene            16565..16789
                     /locus_tag="ACFB78_000488"
     CDS             16565..16789
                     /locus_tag="ACFB78_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC1268556.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            16820..17026
                     /locus_tag="ACFB78_000489"
     CDS             16820..17026
                     /locus_tag="ACFB78_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268557.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            17136..18632
                     /locus_tag="ACFB78_000490"
     CDS             17136..18632
                     /locus_tag="ACFB78_000490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC1268558.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            18642..20087
                     /gene="nuoN"
                     /locus_tag="ACFB78_000491"
     CDS             18642..20087
                     /gene="nuoN"
                     /locus_tag="ACFB78_000491"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC1268559.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFVVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            20199..20498
                     /locus_tag="ACFB78_000492"
     CDS             20199..20498
                     /locus_tag="ACFB78_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC1268560.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(20615..21511)
                     /locus_tag="ACFB78_000493"
     CDS             complement(20615..21511)
                     /locus_tag="ACFB78_000493"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC1268561.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKTGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(21495..21719)
                     /locus_tag="ACFB78_000494"
     CDS             complement(21495..21719)
                     /locus_tag="ACFB78_000494"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC1268562.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(21832..22755)
                     /gene="rsgA"
                     /locus_tag="ACFB78_000495"
     CDS             complement(21832..22755)
                     /gene="rsgA"
                     /locus_tag="ACFB78_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC1268563.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(22760..24616)
                     /locus_tag="ACFB78_000496"
     CDS             complement(22760..24616)
                     /locus_tag="ACFB78_000496"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1268564.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(24642..24779)
                     /locus_tag="ACFB78_000497"
     CDS             complement(24642..24779)
                     /locus_tag="ACFB78_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268565.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(24779..25363)
                     /gene="ruvA"
                     /locus_tag="ACFB78_000498"
     CDS             complement(24779..25363)
                     /gene="ruvA"
                     /locus_tag="ACFB78_000498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC1268566.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDRLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(25445..25771)
                     /locus_tag="ACFB78_000499"
     CDS             complement(25445..25771)
                     /locus_tag="ACFB78_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC1268567.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLKHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(25832..26518)
                     /locus_tag="ACFB78_000500"
     CDS             complement(25832..26518)
                     /locus_tag="ACFB78_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC1268568.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(26646..27110)
                     /gene="trmL"
                     /locus_tag="ACFB78_000501"
     CDS             complement(26646..27110)
                     /gene="trmL"
                     /locus_tag="ACFB78_000501"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC1268569.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(27175..27900)
                     /locus_tag="ACFB78_000502"
     CDS             complement(27175..27900)
                     /locus_tag="ACFB78_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC1268570.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCNTDLASFR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            27899..28648
                     /gene="bioH"
                     /locus_tag="ACFB78_000503"
     CDS             27899..28648
                     /gene="bioH"
                     /locus_tag="ACFB78_000503"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC1268571.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            28645..29433
                     /locus_tag="ACFB78_000504"
     CDS             28645..29433
                     /locus_tag="ACFB78_000504"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1268572.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLADMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCAGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(29514..29621)
                     /locus_tag="ACFB78_000505"
                     /pseudo
     CDS             complement(29514..29621)
                     /locus_tag="ACFB78_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            29743..31116
                     /locus_tag="ACFB78_000506"
     CDS             29743..31116
                     /locus_tag="ACFB78_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC1268573.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(31153..33456)
                     /gene="recQ"
                     /locus_tag="ACFB78_000507"
     CDS             complement(31153..33456)
                     /gene="recQ"
                     /locus_tag="ACFB78_000507"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC1268574.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(33519..34301)
                     /gene="trpC"
                     /locus_tag="ACFB78_000508"
     CDS             complement(33519..34301)
                     /gene="trpC"
                     /locus_tag="ACFB78_000508"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC1268575.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(34352..35308)
                     /locus_tag="ACFB78_000509"
     CDS             complement(34352..35308)
                     /locus_tag="ACFB78_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC1268576.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEVSEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(35441..36979)
                     /gene="murJ"
                     /locus_tag="ACFB78_000510"
     CDS             complement(35441..36979)
                     /gene="murJ"
                     /locus_tag="ACFB78_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC1268577.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
BASE COUNT         8026 a         9491 c        10471 g         9034 t
ORIGIN      
        1 aaaatcgagt ttatcctagt tgtccaggac ggccccttaa tttttataac gtcatgaagc
       61 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
      121 tgcagtttga gcaatgcggt gatttcctac ggctaataca aatgtttaca cattaataca
      181 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
      241 cctgaaagga ttgtcgctgg tgtttttcgc ctctggcttc tgcgccctga tttaccaggt
      301 cagctggcag aggcttctat tcagccacat aggtatcgat ttgagttcga ttactgtcat
      361 tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggcggac gcattgctga
      421 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
      481 cggtttggta agcaagggtc tgatttccgg cttggggcat cttttagttg aggctgattt
      541 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
      601 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
      661 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
      721 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gccttaacct
      781 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatggtg aacactaaac
      841 cgaatactag tttgatttat atgctttctt tccttagcgg attattgagc ttgggtatag
      901 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
      961 ttactcttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
     1021 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
     1081 ccgatttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
     1141 acgccggtat cttcattacc ctgtctgccg tcgtcagggg gttgattttc ccacttgtac
     1201 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttcgcca
     1261 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
     1321 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
     1381 caccgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
     1441 gcatcctcat gttcctactg ccggattccg tctttcaaaa tattgctgac cgtccggata
     1501 ggctgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
     1561 tttatggggc gaatgtatac gacggcgcat acaataccga tatattcaat agtgtcaacg
     1621 gcatcgaacg tgcctatctg ccaccctccc tgaagtccgg catacgccgc attttcgtcg
     1681 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
     1741 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggacgag ccgcaaatcg
     1801 caccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
     1861 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa ttcgacttgg tactggcgtg
     1921 cctattccac taacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
     1981 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
     2041 acagtattcc ctatgcatac cgctacgggc atatggtagt cggctcggca accccggtag
     2101 ttttccctaa taaagaactg ctcaagcaac gcctttcccg gttgatttgg ccggaaagcg
     2161 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
     2221 tgctgattcg gatgacggaa ccttcggctg gggcggaagt cattactgac gataatatga
     2281 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
     2341 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
     2401 tttaaacatt ttgtcccgaa ttcttattcc tatatatagc ggattaacaa aaatcaggac
     2461 aaggcggcgg gccgcaggca gtacaaatag tacggaaccg gttcgcttgg tgcttgggca
     2521 ccttagggaa ccgttcccct tgagctaagg cggggcaacg ccgtaccggt ttttgttaat
     2581 ccgctatacc acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat
     2641 cgttatcata acatgattgt atttattggg tttttttggg cgttttgccg atatttacct
     2701 tttaatggtt tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat
     2761 ttgggtgtaa ccatgttgtc cgcttacttt cccgtctttg tctttatcct cgtcggcctc
     2821 gcggccggcg tactgtttat cctgctcggc acgattttgg gcccgaaacg ccactatgcc
     2881 gaaaaagacg cgccttacga atgcggtttt gaagcctttg aaaacgcaag gatgaagttc
     2941 gacgtgcgct attacctcgt cgccatcctg ttcatcctct tcgatttgga ggttgcgttt
     3001 atgctgccgt gggcagtcgt gttcaaagat ttgggcgcgt acggcttttg gtctatgctg
     3061 gtgtttatcg tcgtcctgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa
     3121 tgggaataga aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga
     3181 actatatgcg taccggttca ttgtggccgg ttactttcgg attggcctgc tgcgccgtgg
     3241 aaatgatgca tgcgggtatg gcgcgttatg accttgaccg tttcggtatc attttccgtc
     3301 cgtccccacg tcaggccgac ctgatgattg tggcaggtac gctgaccaat aaaatggcgc
     3361 cagccctgcg tcgcgtgtac gaccagctcg ccgagccgcg ctgggtattg tctatgggtt
     3421 cgtgtgccaa cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgccgacc
     3481 gtgtcgtgcc ggtggacgtt tatgtgccgg gttgtccgcc gactgcggaa gctctgattt
     3541 acggcctgat tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt
     3601 aaggagagga cgatatggca agcattcaaa acttatacga gaccgtcgtc ggcgtgcttg
     3661 gcgatcaggc aggcaaagtc atttcagctt tgggcgagat taccgtcgag tgtctgcccg
     3721 agcactatat ttcagtcatg accgcattgc acgaccatga agatttgcat ttcgagcttc
     3781 tggttgactt gtgcggcgtc gattacagca cttacaaaaa cgaagcatgg cagggcaaac
     3841 gctttgccgt tgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg
     3901 tctgggtttc agacgacgac ttccctgtag tcgaatccgt agccgatatt tacaacagcg
     3961 cggattggta cgagcgcgaa gccttcgatt tgtacggcat catgttcaac aaccatcccg
     4021 acttgcgccg catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc
     4081 cgatttccgg ctatgtggaa atgcgttatg acgaagagca aaaacgcgtg atttaccaac
     4141 ctgttaccat tgagccgcgc gaaatcacac cgcgtatcgt ccgtgaggag aactacggtg
     4201 gccaataaat taagaaacta caccatcaac ttcggcccgc agcaccctgc ggcgcatggc
     4261 gtattgcgta tgattttgga gctggacggc gaacaaatcg tccgtgccga tccgcatatc
     4321 ggcctcctgc accggggcac cgaaaaactg gcggaaacca aaacctatct gcaagccctg
     4381 ccctatatgg accgtttgga ctacgtttcc atgatggtca acgagcaggc gtattgcttg
     4441 gcggtagaaa aacttgccgg tatcgatgtg cctatccgcg cccaatacat ccgcgtgatg
     4501 ttcgccgaag taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgccttcgac
     4561 atcggcgcga tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg
     4621 tacgaagccg tgtccggcgc acgtatgcac gccgcctact tccgtcccgg cggcgtttac
     4681 cgcgacctac ccggctttat gcccaaatac gagagcagca aattccgcaa tgccaaagta
     4741 ttgaagcagc tcaacgaatc ccgcgaaggc acgatgcttg actttatcga tgccttctgc
     4801 gaacgcttcc ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgcatctgg
     4861 aaacagcgta ccgtcggcat cggcgttgtc tctcccgagc gcgccatgca aaaaggcttt
     4921 accggtgtga tgcttcgcgg ttccggcgtg gaatgggacg tgcgtaagac acagccctac
     4981 gaagtgtacg acaaaatgga tttcgacatc cccgtcggcg tcaacggcga ctgctacgac
     5041 cgttacctct gccgtatgga agaaatgcgc caatccgtac gcatcatcaa acaatgcgcc
     5101 gactggttgc gcgtcaatcc cggcccggtc attaccgcaa accacaaatt cgctccgccc
     5161 aaacgtaccg aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc
     5221 gagggtatgc acgttcccga gggcgagacc tacaccgctg tcgaacaccc gaaaggcgag
     5281 ttcggcgttt acatcatttc agacggcgca aacaaaccct accgcctgaa aatccgcgca
     5341 cccggcttcg cccacctgca aggcatggac gaaatggcaa aaggccatat gctcgccgac
     5401 gtcgttgcca tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat
     5461 ccgcaaaatc cttaaaacaa atcgacattg agttggcgaa atatcccgcc gaccaacgcc
     5521 gttccgccat catgggcgcg ttgcgtatcg cccaaaccga aaaaggctgg ctcgcccccg
     5581 aaaccatcgc ttttgttgcc gactacatcg gcatcacgcc ggcgcaggca tatgaagtcg
     5641 ccactttcta caatatgtac gaccttgagc ctgtcggcaa atacaaactg accgtttgta
     5701 ccaacctgcc ctgcgctctg cgcggcggta tggcgaccgg cgaatacctc aaacaaaaac
     5761 tcggtatcgg ctacggcgaa accacgcccg acggcaaatt tacccttgtc gaaggcgaat
     5821 gcatgggcgc gtgcggcgat gcccccgtta tgctggtcaa caaccacagc atgtgcagct
     5881 ttatgaccga agaggcgatt gagaagaaac tggcggagtt ggagtaggtc gtctgaaacg
     5941 acgatttaaa cgtaggtcgg atacttgtat ccgacagagt gggtaaaaag gcaaaatgtc
     6001 ggatttaaga atccgcccta ctgaaatact gaaatgccgt cattcccgcg caggcgggaa
     6061 tccaccggta agattcggtt tctgaattta ataagacatt gcttaccatt gaggatggat
     6121 tcccgcctgc gcgggaatga cggcagacaa gcaagtggtc gagatccaac aaaaacgatt
     6181 gaaggtcgtc tgaaaatatc gatttgataa actagatttt atttcagaag acgttacaag
     6241 ccgatacaca aagacatttt aaggtcgtct gaaacagcgg ccgcaaccga tacgaaaaca
     6301 aacaggcaca ccaaaaatgg ctatttacca atcaggcgtg atttttgacc aagtggatac
     6361 cgccaatccc gattgctgga cattggacga atacgtcaaa cgcggcggct ataccgccct
     6421 gcgtaaaatc ctgtccgaaa acatctcgca aaccgatgtg attgacgaag tcaaaacctc
     6481 cggcttgcgc gggcgcggcg gtgcgggctt cccgaccggt ttgaaatgga gctttatgcc
     6541 ccgttctttc ccgggcgaaa aatatgtggt ttgcaacacc gacgaaggcg agccgggtac
     6601 atttaaagac cgcgacatca tcatgttcaa tcctcatgcc ctgattgaag gcatgatcat
     6661 tgccggttac gcgatgggcg cgaaagccgg ctacaactat atccacggcg aaattttcga
     6721 aggttatcaa cgctttgaag ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa
     6781 aaatattttg ggttcggatt ttgaattcga actcttcgcc caccacggct acggcgcata
     6841 tatttgcggc gaagaaaccg cattgcttga atcgttggaa ggcaaaaaag gccagccgcg
     6901 cttcaaaccg ccgttccccg cctcgttcgg cctgtacggc aaaccgacca ccatcaacaa
     6961 taccgaaacg ttctcctccg ttccattcat tatccgtgac ggcggacagg cgtttgccga
     7021 taaaggtatt ccgaatgcag gcggtaccaa attattttgt atttccggcc atgtcgagcg
     7081 tccgggcaac tatgaagtgc cgttgggcac gccgtttgcc gaagtcttga aaatggcggg
     7141 cggtatgcgc ggcggtaaaa aactcaaagc cgttattccc ggcggttcgt ccgcccccgt
     7201 attgcctgcc gacattatga tgcagaccaa tatggactat gactcgattt ccaaagccgg
     7261 ctcgatgctc ggttccggcg cgattatcgt gatggacgaa gacgtgtgca tggtcaaagc
     7321 cctcgaacgc ttaagctatt tctactacga cgaatcctgc ggccaatgca ccccctgccg
     7381 cgaaggtacc ggctggcttt accgcatcgt ccgccgcatc gtagaaggca aaggccgcat
     7441 ggaagacttg gatttgctgg attctgtcgg caaccaaatg gcaggccgca ccatctgcgc
     7501 ccttgccgat gccgccgtct tccctgtccg cagctttacc aagcatttcc gtgatgagtt
     7561 tgcgcattac atcgaacacg gcgggccgat gaaagagcat aagtggggag ggtggtaatg
     7621 gtggaagcta aaatttttat tctatacggt gcagccaaca aaggtaagag tacgacactc
     7681 aatacgcttt ttaatcagat ttgtcggaaa ttttctaaat ttctagtctt ttttgaaaga
     7741 tatggaaacg gcttagattt tgttgcagta tttgatcatg aaggtcagag gattggtttt
     7801 tattcatctg gtgataatga atacgaggtt aggagaaatt tatacaaact ttattcgcat
     7861 aattgtgatt ttattttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat
     7921 attgcatggc gatgtaaata taattaattg gtgtgaaaag tttgagccta cagatgaaga
     7981 caatgagcgt gctgttaaag agttatttaa gtcatttaaa aatataataa atgagttata
     8041 gttttagttg gttttatatt ggttaaaagc aaaatgctaa aaatttaact ttgccgtcat
     8101 tcccgcacag gcgggaatcc atagtggaat ttacagaacc cgatatttga aaagcagttg
     8161 ccgaaattca aaaaatggat tcccgcctgt gcgggaatga cggcaaggtg gcagtaaacg
     8221 ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaagccc atttttcaga
     8281 cgatctccat aaaaaattat ttatcaaata cccgtaacta ggaacgaacc atgttacaaa
     8341 tcgaaatcga cggcaaacag gtatctgtgg agcagggcgc gacggtaatt gaagccgcgc
     8401 acaagctcgg tacttatatt ccgcatttct gttaccacaa aaagctctct attgccgcca
     8461 actgccgtat gtgtctggtg gatgtggaaa aagcccccaa accgctgcct gcctgtgcca
     8521 cgccggtaac ggacggcatg attgtgcgta cgcattcggc aaaagcccga gaggcgcaag
     8581 aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc tgcgaccagg
     8641 gcggcgaatg ccagttgcag gatttggcgg tgggctacgg caaaaccacc agccgttata
     8701 ccgaagaaaa acgttccgtc gtcggcaagg acatgggtcc tttggtttcc gccgaggaaa
     8761 tgagccgctg tatccactgt acccgctgcg tacgtttcac cgaagaaatc gccggtttgc
     8821 aggaaattgc gatggtgaat cgtggtgaac actccgaaat catgcccttt atcggcaaag
     8881 cggtggaaac cgagctgtcg ggcaacgtca tcgatttgtg tcccgtcggc gcattgacca
     8941 gcaaaccgtt ccgcttcaac gcgcgtactt gggaattgaa ccgccgcaaa tccgtttccg
     9001 cacacgacgc tttgggcagc aacctgattg tacaaaccaa agaccatacc gtccgccgcg
     9061 tgttgccgtt ggaaaacgaa gccatcaacg aatgctggct gtccgaccgc gaccgttttg
     9121 cctacgaagg cctgtatcac gaaagccgtc tgaaaaatcc gaaaatcaaa cagggtggcg
     9181 agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg attgaatgta
     9241 tcgccaaaga cggcaaccaa aaccaagtcg gcgtttgggc taatccgatg aatacggttg
     9301 aagagctgta tctggccaaa aaatttgccg acggcttggg tgttaaaaac tttgcaaccc
     9361 gtttgcgcca acaagacaaa cgtctttcag acggccttaa aggtgcgcaa tggttgggac
     9421 aaagtattga atctttggct gacaacgatg ccgtattggt agtcggtgcg aacttgcgca
     9481 aagaacagcc gctcctgacc gcccgcctgc gccgcgccgc caaagaccgt atggccttga
     9541 gcgtattggc cggcagtaaa gaagaattgt ttatgccgct tctgtctcaa gaagccgcac
     9601 atcccgacga gtgggcaggc cgtctgaaaa acctgtctgc cgatgcggaa catgccgtta
     9661 ccgccagcct gaaaaacgct gaaaaagcag cggtgatttt gggtgcggaa gtgcaaaacc
     9721 atcctgatta cgccgccatt tatgccgccg cgcaagagtt ggcggacgcg accggcgcag
     9781 tgctgggcat tttgccgcaa gccgccaaca gcgtcggtgc ggacgtattg ggtgtgaact
     9841 cgggcgagag cgttgccgaa atggcaaaca cgccgaaaca ggcagtcttg ctgctcaacg
     9901 tcgagcctga aatcgatacg gttgacggtg caaaagccgt agccgcgttg aaacaggcga
     9961 aaagcgtgat ggcgtttacg ccgtttgtca gcgaaacgct gctggacgtg tgcgacgtat
    10021 tgctgccgat tgcgccgttt accgaaacat caggcagctt catcaatatg gaaggccgtc
    10081 tgcaatcttt ccacggcgtg gtacaaggct tcggcgattc acgtccgttg tggaaagtgt
    10141 tgcgcgtatt gggcaacctg tttgacctga aaggttttga ataccacgat accgccgcga
    10201 ttctgaaaga cgcgctggat gcggaaagcc tgccgtccaa actggacaac cgcagcacat
    10261 gggcagggga gggcgttcag acggcctcaa accgcctcgt ccgtgtcggc ggcgtcggta
    10321 tttatcatac tgattctatc gtgcgccgtt ccgcaccgtt gcaagaaacc agccatgccg
    10381 ccgtgcctgc cgcgcgtgta aatccgaaca cattggcacg cttgggcctg caagacggac
    10441 aaaccgctgt cgccaaacaa aacggcgcaa gcgtatcggt tgccgtcaaa gccgatgccg
    10501 gtttgcctga aaacgtggtg catctgccgc tgcataccga aaatgccgcg ctgggtgcgt
    10561 tgatgggcac tattgaactg gcgggagctt gattatgcag gaatggttcc aaaacctctt
    10621 tgccgcaacg ctcggtctgg gcgatttggg catcaccgta ggcttggtgg tatccgtcat
    10681 cgtcaaaatc gtgattatcc tgattccgct gattctgacc gttgcctacc tgacgtattt
    10741 cgaacgtaaa gtcatcggct ttatgcagct tcgcgtcggc ccgaacgtaa ccggcctgcg
    10801 gggtctgatt cagccgtttg ccgacgtgtt caaactcttg tttaaagagg taacccgtcc
    10861 gaagctgtca aacaaagcct tattctacat cggcccgatt atgtcgctcg ccccgtcttt
    10921 cgcggcgtgg gcggtgattc cgtttaacga agaatgggtg ctgaccaata tcaatatcgg
    10981 ccttttgtac atcctgatga ttacctcgct gtcggtttac ggcgtgatta tcgcgggctg
    11041 ggcttccaac tccaaatatt cgttcttggg tgcaatgcgt gcttccgcgc aaagcatttc
    11101 ctacgagatt gccatgagtg ccgcgctggt gtgcgttgtg atggtatcgg gcagcatgaa
    11161 cttctccgac atcgttgccg cgcaggcaaa aggcatcgcg ggcggttctg tgttctcgtg
    11221 gaactggctg ccgctgttcc caatcttcat cgtctatctg atttccgccg ttgccgaaac
    11281 caaccgcgca ccgtttgacg tggcagaggg cgagtctgaa atcgttgccg gacaccacgt
    11341 cgaatactcc ggctttgcat tcgcgctgtt cttccttgcc gaatacattt tcatgattct
    11401 gattgccgcg ctgacatcgc tgatgttcct cggcggctgg ttgtctccgt tcccgcaaag
    11461 ctggggcatt gtcggtacgc cttccgcatt ctggatgttc gtgaaaatgg cggcggtgct
    11521 gtactggtat ctgtggattc gtgcaacctt cccacgctac cgctacgacc aaatcatgcg
    11581 tttgggctgg aaagtgctga ttccgatcgg cttcgcctac atcgtggttt tgggcgtgtg
    11641 gatgatttca ccgctgaatt tgtggaaata agtttcagac ggcatcttga ggccgtctga
    11701 acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa tatggctaac
    11761 ttagtaaaaa cctttctgct tggcgaactg gtcaaaggca tgggcgtaac gctcaaaaac
    11821 ttttttgccc gcaaagacac aatttatttt cccgaagaga aaacgccgca atccgtgcgt
    11881 ttccgcggtc tgcacgcgca acgccgttat ccgaacggag aggaacgctg catcgcgtgt
    11941 aaattgtgcg aggcggtgtg tccggcaatg gcgatcaaca tcgaatcgga agaacgcgaa
    12001 gacggcaccc gccgcaccaa gcgttacgac atcgacctga ccaagtgcat cttctgcggt
    12061 ttctgcgaag aggcctgccc gaccgatgcg attgtggaaa cccatatttt tgaataccac
    12121 ggcgagaaaa aaggcgactt gcatatgacc aagcccatcc tcttggcaat cggcgacaaa
    12181 tacgaagccg aaatcgccaa acgcaaagcc gctgacgcgc cgtaccgcta aggagcagac
    12241 gaatgacttt ttccgtgatt ctgttctata tccttgccgc catcgttttg tacggtgcgg
    12301 ttcgtaccgt taccgccaaa aaccctgtcc atgccgcttt gcatctggtg ctgaccttct
    12361 gcgtgagcgc gatgatttgg atgctgatgc aggcggagtt tttgggcgtg acgctggtgg
    12421 tggtttacgt cggcgccgta atggtgttgt tcctgttcgt cgtgatgatg ctgaatatcg
    12481 acatcgagga aatgcgcgcc ggtttctggc ggcacgcgcc tgttgccggt gtggtcggta
    12541 cgttgttggc ggttgccctg atcctgattc tggtcaaccc gaaaaccgac ctggccgcat
    12601 ttggtctgat gaaagacatt ccggccgatt acaacaatat ccgcgatttg ggcagccgta
    12661 tttataccga ctacctgttg ccgtttgaat tggcggcggt attgctgctg ttgggcatgg
    12721 tggccgcgat tgcattggtt caccgcaaaa ccaccaatcc gaaacgtatg gatccagccg
    12781 accaagttaa agtacgcgcc gatcaaggcc gtatgcgtct ggtgaaaatg gaagcggtca
    12841 aaccgcaagt cgaatctgcc gaagaaagcg aagtttcaga cggcctcaag acggaagggg
    12901 agggcaaagc atgattactt tgacgcatta tctggtattg ggcgctctcc tgttcggtat
    12961 cagcgcgatg ggcatcttta tgaaccgcaa aaacgtgctg gtattgctga tgtccatcga
    13021 gctgatgctt ttggcggtga acttcaactt tatcgccttc tcgcaacatt tgggcgatac
    13081 tgccggacaa attttcgtat tcttcgtatt gaccgttgcc gctgccgaat ctgccatcgg
    13141 tttggcgatt atggtgctgg tgtaccgcaa ccgacaaacg attaatgttg ccgatttgga
    13201 cgagttgaaa gggtaatcat gaacgatatg actttatatt tgataattgc ccttgttccg
    13261 ttggcaggct cgctgattgc gggtttgttc ggcaacaaaa tcgggcgtgc cggcgcgcat
    13321 acggttacga tactcggtgt cgctgtatct gccgtgctgt cggcttatgt gctgtgggga
    13381 ttcctcaatg gcagccgtac caagtttgac gaaaacgtct atacctggct gacaatgggc
    13441 ggcttggatt tttccgtcgg cttcttggtc gatacgatga cggcgatgat gatggtcgtg
    13501 gtaacgggcg tgtcgttgat ggtgcatatc tataccatcg gttatatgca cgatgaaaaa
    13561 gtcggctacc aacgcttctt cagctatatt tctttgttta cattcagcat gttgatgctg
    13621 attatgagca acaacttcat ccagctcttc ttcggttggg aagcggtggg cttggtgtcg
    13681 tatctcttga tcggtttcta tttcaaacgc ccgggcgcga catttgccaa cctgaaagcc
    13741 tttttgatca accgtgtcgg cgacttcggc tttttgctcg gtatcggctt ggtgcttgcc
    13801 tatttcggcg gcagcctgcg ttatcaggac gtattcgcct atctgcccaa cgtacaaaat
    13861 gccaccatcc aactgtttcc cggtgtggaa tggtctttga ttaccgtaac ctgtttgctc
    13921 ctgtttgtcg gtgcgatggg taaatcggca caattcccgc tgcacgtctg gctgcccgat
    13981 tcgatggaag gcccgactcc gatttctgca ttgattcacg ccgcgacgat ggttaccgcc
    14041 ggtttgttta tggtgtcgcg tatgtcgccg atttatgaga tgagcagcac cgcgctgtcg
    14101 gtcattatgg taatcggcgc gattaccgcc ctgtttatgg gcttcttggg tgtgattcaa
    14161 aacgacatca aacgtgtggt tgcgtattct accctgtcgc aactgggcta catgaccgtg
    14221 gctctgggcg cgtccgccta ttctgtggcg atgttccacg tcatgaccca cgccttcttt
    14281 aaagccttgc tgttcttggc ggcgggcagt gcgattatcg gtatgcacca cgatcaagat
    14341 atgcgccata tgggcaacct gaaaaaatac atgccgatta cttggctgac tatgctgatc
    14401 ggcaatttgt ctctgatcgg tacgccgttc ttctccggct tctactccaa agattcgatt
    14461 atcgaagcag tgaaatacag caccctgccg ggcagcggct ttgcctattt tgccgtcctc
    14521 gccagcgtgt tcgttaccgc gttttacgcg ttccgccaat actttatggt gttccacggc
    14581 gaagaaaaat ggcgcagcct gcccgaacac cattcagacg gccatggtga agaacatcac
    14641 ggcttgggca aaaacgacaa tccgcacgaa agcccgctgg tcgttaccct gccgctgatt
    14701 ctgcttgcga ttccgtccgt catcatcggc tacatcgcca tcgaacccat gctctacggc
    14761 gatttcttca aagacgtgat tttcgtcaac gccgacgcgc atccgaccat gcacatcatg
    14821 aaggaagagt tccacggcgc attggcgatg gtgtcgcaca gtctgcattc gcccgtcctc
    14881 tatctcgcta ccgcaggcgt attgagcgca tggcttttgt acgtcaaact gccgcacctg
    14941 cccgctaaaa tcgcgcaggc gttccgtccg gtttacgttt tgtttgaaaa caaatactac
    15001 ctcgacgcct tgtatttcaa cgtcttcgcc aaaggcacgc gcgcattggg aaacttcttc
    15061 tggaaagtcg gcgataccgc cattatcgac aacggcatcg tcaacggctc cgccaaactg
    15121 gtcggcgcaa ttgccgcgca agtgcgtaaa gtccaaaccg gctttatcta cacctacgcc
    15181 gccgctatgg tgttcggcgt attggtattg ctcggcatga ccttctgggg attgttccga
    15241 taaggatgag gcttcagacg gcctgcttat cgctggtgta gaggtcgtct gaaaagaagg
    15301 tttcaggcgg caatagatta gagtttcaga cgacatctga catttcagat agtcaggccg
    15361 tctgaaaaat agaagggcgg ataatatgga taaacggctg tttgacggta cggttcgttt
    15421 tgagcatgta accggtgtcg gcacgatgca ggtagatttc cagcccaatc agcgtgttta
    15481 tacgatgatt ggcgagaatg gtgtcggtaa gaccaaattt ttggaatgtt tgtttacggt
    15541 attgttgttt acttatcgaa tggcgaacat aaacataggt tttagttgga tagatttgca
    15601 acgtttacct ttgaaaatag ttgaattgcc taatggaaaa aatgaggatt ttaggcgctt
    15661 ttcagagcgt agtaatcagg gctttaatat taacgaagac gttttgttct caggatatca
    15721 acatcgactt ccttttgtat atttgccggc acagagtcgt gggtgggtaa aaaaacaaac
    15781 ttccaaactc agtcgctggg aagttctcaa gggcgtgcaa gcaaatatct ggggaatttg
    15841 ttttctgttt ttcaatctgg ccatagttca ttctcgctta aatccttaaa tatggaaaac
    15901 gatttagaag aatggattat ccagcgcgcc cgatcagcca atccgtatca ggcaaacgaa
    15961 gataatcgaa aaattgaaat tgatacttta ctgtttcttc ttaatcaatt tgatggtcgt
    16021 attgcacctg attttttaga aattaaagga gatgatcgta tattcatcaa gttggataat
    16081 caaaaacgcg aattgtcagg attatccagc gggtttacgt ctattttgaa aattatgcaa
    16141 tctattgttg cgggatacag ttactttacc aatgagcagc aaattgccaa tgtgcgcggc
    16201 gtggtgctga ttgacgaaat agaaagccat ttgcacaacg aatggcagat caaaatcgtg
    16261 ccgctgctga aaaaattgtt cccgaacaca acgtttttca ttaccacgca ttcttcttta
    16321 gtcatcagtc agttagagca gggaggggcc tatcgtttgg tacgtagcaa ggaggacggt
    16381 gtagtctatg gcaagatgat tgactatcca agtaatgctt cttttgtgga tttgctcaac
    16441 gaagcattcg gcgtggattt gaataagaag aagatagaac gcgcccaaga acgtaatcaa
    16501 gaacaggcaa aaaagcctta ttggaattgg tacgtcagga attgggcgga ttaggagata
    16561 aataatgacg gtttatcttt tagataccaa ttacttggtt tatttggcgg atgatgacag
    16621 tgacgaagaa aagcggaaag ccgtattgag cgatatggcg gaaaagttgc agcaggatga
    16681 caaccgtttt gtgattaccc cgcttatccg ttatgaagtt ttgcgcggtg tggattgggg
    16741 aaaatctgaa aaattgtctc gattgaccgg tgttttggca cagttttagt ctttggacat
    16801 tacacagaat gtttctgatt tggcaagtaa tctgtaccgt tttgataagt ttgaagccga
    16861 acgtgataac acgccgaaga atttagaaaa acgtaaattc gatatgtttc attatgcgac
    16921 tgccagtgtg aataatttag aaatcttatc ccacgatacc gatgttaata agattaaaga
    16981 tttacatgaa agaatgaggc tggaagattc ggcagaatta gcttgagaca aatagcagaa
    17041 acctgaacaa acctttttca gacggcctct taaaacgtcg tctgacaaac caacccgtac
    17101 aacgatattt ttattttaac cacaggttaa ccactatgtt ttccaactac ctactcagct
    17161 tggcaatatg gatacccatc gccgcaggcg tgctggtttt ggcgaccggt aaagacagcc
    17221 gtgcgccgct ggcgcgtgtg cttgccttca tgggagcgct tgccggtttc ttggtaacgc
    17281 tgcccctgtt taccggtttc gaccgtttga gcggcggcta tcaatttacc gagttccacg
    17341 agtggattcc gcttctgaaa atcaactacg cattgggcgt ggacggtatt tcagtgctct
    17401 ttatcatctt gaatgcgttt attacgctgc tggtggtatt ggccggttgg gaagtcattc
    17461 agaaacgtcc ggcgcagtat atggcggcat tcctgatgat gtcgggtttg attaacggcg
    17521 cgtttgccgc gcaggatgcg attctgtttt atgtgttctt cgagggtatg ctgattccgc
    17581 tgtacctgat tatcggtgta tggggcggcc cgcgccgcgt ctatgcgtcg gtcaagctgt
    17641 tcctctatac gctgacgggt tcgctcttga tgttggttgc catggtttac ctgtactatc
    17701 agacaggcag cttctctatt gtcgatttcc aaaacatcaa acagattccg ttgggcgtac
    17761 aacagctttt gtttgtagcg ttcttcctgt catttgccgt aaaagtgccg atgttccccg
    17821 tacacacttg gctgccggat gcccacgttg aagcgccgac cggcggttcg atggtgttgg
    17881 cggcaattac gctgaaactg ggtgcgtatg gtttcttgcg ctttatcctg ccgattatgc
    17941 cggacgcggc tcgctatttt gcccccgtga tcatcgtatt gagcctgatt gccgtaattt
    18001 atatcggtat ggtggccctg gtgcaaaccg atatgaagaa actggtggcg tattcgtcca
    18061 tcagccatat gggttttgta acgcttggga tgtttttgtt tgttgacggg cagttggacg
    18121 actgggcgtt gaaaggcgcg gtcattcaga tgatttcgca cggcttcgtg tcggcggcga
    18181 tgtttatgtg catcggtgtg atgtacgacc gcctgcacac gcgcaatatt gccgattacg
    18241 gcggcgtggt caatgtgatg cccaagtttg cggcgtttat gatgttgttc ggtatggcga
    18301 acgccggcct gcctgcgact tccggcttcg tgggcgagtt tatggtggtt atgggcgcgg
    18361 tcaaagtgaa tttctgggtg ggcgcgctgg cggctatgac gttgatttac ggcgcgtctt
    18421 ataccctgtg gatgtacaag cgcgtgattt tcggcgcgat tcacaatccg cacgttgccg
    18481 aaatgaaaga catcaattgc cgcgagtttg cgattctggc ggttttggcg gttgctgttt
    18541 tggggatggg cctgtatccg aacgcattta tcgaagtggt gcatcaggcg gcaaacgatt
    18601 tgattgccca tgtggcgcaa agcaagattt gaggtgtgta aatgaattgg tcagatttga
    18661 atttaatgcc cgcactgccc gaagtcgcgc tgctgtcgct gctggtgtta ttgttgccgg
    18721 cggacttgtg ggccagtgat gacaaatgcc gttggacgca ttacggcgcg ttggcaacgg
    18781 tggcggttac ggcggcggtg cagcttgccg tgtgggaaca gggcagcacg tcttcgttca
    18841 acgggatgta tattgcggac ggtatgtcgc gtttggcaaa aatggtttta tatgccttga
    18901 cgtttgtcct gtttgtctat gccaagccct acaaccaagt gcgcggtatt ttcaaaggcg
    18961 agttttacac cctgtcgctg tttgccctct tgggcatgag cgtgatggtg agcgcggggc
    19021 attttttaac tgcctatatc ggtttggaac tcttgtcgct cgccctttac gccctgattg
    19081 ccctgcgccg cgattccggt tttgccgccg aagccgcctt gaaatatttt gttctgggcg
    19141 cgctggcatc cggcctgctg ctctacggta tttctatggt ttacggcgca accggttcgc
    19201 tggaatttgc cggcgtgctc gcctcttcct tcaatgaaga agctaacgaa tggctgttga
    19261 aactgggtct ggtgtttgtc gtcgtcgccg ttgcgttcaa actcggcgcg gtaccgttcc
    19321 atatgtggat gcccgacgtg tatcacggcg cgcccacttc tgttaccgcc ttggtcggca
    19381 ctgccccgaa aatcgccgcc gtcgttttcg ctttccgcat cctcgttacc gggctgggaa
    19441 ccgtgcatca tgactggtct ctgatgtttg ccctgcttgc cgccgcctcg ctgctggtcg
    19501 gcaaccttgc cgccatcatg cagaccaata tcaaacgtat gctcgcctat tccaccgtat
    19561 cgcatacggg tttcatcctg ttggcgttta tggcgggcgc ggtcggcttt gcggcgggcc
    19621 tctattacgc cattacctac gcgctgatgg cggcggcagg gttcggcgtg ttgatggtgt
    19681 tgtcggacgg ggacaacgag tgcgaaaaca tcagcgattt ggcagggttg aaccaacacc
    19741 gcgtatggct tgcctttttg atgctgctgg ttatgttctc tatggcgggc attccgccgc
    19801 tgatgggttt ttacgccaaa ttcggcgtga ttatggcgct tttgaaacaa ggctatgtat
    19861 ggttgtcggt gtttgccgtc gtgatgtcgc tggttggcgc gttctactac ctgcgcgtgg
    19921 tcaaagtcat gtattttgac gaatccggcc gcgcccgtcc cgccgccggc ggcaacaatg
    19981 ccgcaaaatc cctcttaagc gtcaacgcgc tgctgttggt gctgtggggc attatgccgc
    20041 aaaccgttat cgactggtgc gccaaggcgt tggagaacac gctgtgagcc gccgcaacgg
    20101 cagccgtgtc agaggctgcc gtttttgtta agatatgccg ttccgcaatg cagttcagac
    20161 ggcatcgccg ccgacaatgc ccaaacagaa agcccatcat gaccgcatcc atgtacatcc
    20221 ttttggtctt ggcactcatc tttgccaacg cccccttcct cacgaccaga ctgttcggcg
    20281 tggccgcgct caagcgcaaa catttcggac accacctgat cgagctggcg gcaggtttcg
    20341 cgctgaccgc ctctcttgcc tacatcctcg aatcccgtgc gggagcggta cacaatcagg
    20401 gttgggagtt ttacgccacc gtcgtctgcc tgtacctcat tttcgccttc ccgtgtttcg
    20461 tgcggcggta tttttggcac acgcgcaaca gggaataaac aagcaaacaa gcataggaat
    20521 gccgtctgaa accctttcag gcggcatttg tttcattcaa gtgcaggccg gcatcgctgt
    20581 gccggcacgt ttcagtcggc gatatacgcc ggttttaata tttgcgtgcg actgcaaatt
    20641 ctgccaactg ccgcaggcgc agggctttgt cgccgaaggg ttcgagcagc gcgactgctt
    20701 cggcaaccag tttgtgtgcg tatgagcgcg ccgcttccaa gcccatcagt ttcacataag
    20761 tcggcttgtc gttgtcggca tctttgcccg ccgtcttgcc caaagtcgcc gtgtccgctt
    20821 cacaatccaa cacatcgtcg atgacttgga acgccaaacc cagttttgcc gcgtaagcgt
    20881 ccaatacggc aagttccgcg tcggacagat cgggacacgc cgtcgccccc aataaaaccg
    20941 ccgcacggat caacgccccc gttttcaggc tgtgcatccg ttccaaatcg gcttgaacca
    21001 tttgtttgcc gacatttgcc aaatcgattg cctgtccgcc cgccataccc gcgctgccgc
    21061 ccgtttttgc caacaccgac aacattgcca actggcgtgc ggcgggcagt tctgtcggac
    21121 ggctcaacac gtcgaaggct tgggtttgta aagcgtcgcc ggtcagaagg gcggtggctt
    21181 cgccatattt gatgtggcag gtcggtttgc cgcgccgcag gctgtcgttg tccatcgccg
    21241 gcatatcgtc gtgaaccaaa gaatagacgt ggatcatttc gattgccgcc attgcctgtc
    21301 cgaccgcatc cgccacggcc ccgcccaatt ccgaagccgc cagcaccagc atcgggcgca
    21361 gacgcttgcc gccgtccaaa gccgcatagc gcatcgcttc gtgcagcgtg tgcgggattt
    21421 cgtttccaga cggcaaaaag cgttccagca gcagctctgt ctgcgcctgc gccctctgtt
    21481 gccacgcttt caaatcattc gtcggattca aggttcaact ccttcgtccc gtccgcatcc
    21541 aaaacctgta atttttgttc gacttgggcc agcttggttt ggcagtacct gaccagctcg
    21601 ttgccttcct gataggcggc aagcgcgtct tccaagggca tttcgccctg catggactgc
    21661 gtcagcgatt caaggcgcga caaggcctct tcaaacgatt tcggggtgtt tttcttcata
    21721 gtttcgggtg ttccggtaaa aagcggattg tagcattttt gcaaaacgga tgccgtctga
    21781 aacccgaatc cggcttcaga cggcattttt tccgccccgg tcggcaaggc gttacccgag
    21841 cagttcgtcg gtgatgccct gcaaaaaggc gaggcgttcg gggcttgccg ccccggtttc
    21901 ggcagcggct ttgaaggcgc agccgggttc ggcgcggtgg gtgcagttgt ggaagcggca
    21961 ttgcccgaca aggtggcgga aatcggggaa atagtgcggc aaatcggcgg cttggaggtg
    22021 gtgtaaacca aattcttgca aacccgggga gtcgatgagt tgggtttcgc cgttcaaatc
    22081 ataaagccgg gcgtgggtgg tggtgtgttt tcccgagtcg agtgcggcgg aaatgtcgcc
    22141 ggtgcgggcg gtttggctgc ctaaaagggc gttggtcagg gtggatttgc ccataccgct
    22201 ttgtccgagc aggatgttgc tgtgcccttg caggacgggg cgcaggctgt cggcgttttc
    22261 cagcacgcgg gtttcgatga cggggtaacc cagcgtttcg tagaatttga gtttttcgag
    22321 ccagagggcg gtttcgggca ggtcggcttt gttcaggacg atgacggcgc ggatgccggc
    22381 ggcttcggcg gcaagcaggg cgcgctgtag cagccgcacg ctcggactcg ggacggcggc
    22441 ggttacgatg aggagttggg taacgttggc ggcgatgagt ttggttttcc acgcgtcttg
    22501 gcggtagagc aggctttggc gcggtaaaaa atcttcaatc acaacttgtt cggcgttgac
    22561 ggggctgatg cggacgcggt cgccgcaggc gaaatcgacg cgttttttgc gggtgctggc
    22621 ttcgtaggtt gtgccgtcgg gcgtgcggac gatgtagcgg cggccgtagc cggcggtaat
    22681 ttgggcggtg tcgttcatgg tttctttggg gttgggtgtg gaaatgccgt ctgaaaaggg
    22741 gtgttcggac ggcatcggtt cagtcgtgct gccactcgac gtgttcgctg aggaagccgc
    22801 cgctctggtg cgcccagagt ttggcgtaaa gcccgcgttt ttcgaggagt tcggcgtgtg
    22861 tgccttcttc gatgatgcgg cctttgtcga ggacgacgag cctgtccatc gcggcaatgg
    22921 tggagaggcg gtgggcgatg gcgatgacgg ttttgccttc catcattttg tcgaggcttt
    22981 cttggatggc ggcttcaact tcggaatcga gcgcgctggt ggcttcgtca agcagcagga
    23041 tgggtgcgtc tttgagcatc acgcgggcga tggcgatgcg ctggcgttgt ccgccggaga
    23101 gtttcacgcc gcgttcgccg acgtgcgcgt cgtagccgct ccgccctttg gcatcggaaa
    23161 ggtcggggat gaagccggcg gcttcggcgc gttcggcggc ggaaaccatt tcggcatcgg
    23221 tcgcgtcggg gcggccgtaa ataatgttgt cgcgcacgga acggtgcagc agcgaggtat
    23281 cttgtgtgac caaaccgatt tgggcgcgta aagattcttg ggtaacgccg cttatgtcct
    23341 gcccgtcgat cgaaaccgtg ccgctttgcg gttcgtagaa acgcaaaagc aggttgacga
    23401 tggtggattt gcccgcgccg ctgcgtccga tcaagccgac tttttcgccg ggtttgatat
    23461 tcaggttaaa gccgttgagc agcggtttgc cggcttcgta gcaaaaatcg acgtgttcga
    23521 acttgattgc gccttgcggc acgttcagcg gcagggcttg gggcttgtcg aggatggtgt
    23581 gcggtttgga cagggtcgcc atgccgtcgt tgacgatgcc gatgttttca aacagccgcg
    23641 cggattccca cataatgtat tgcgacagcc cgttgacgcg caacgccatc gcggtggcgg
    23701 tggcgaccgc acccacgccg acctgtccgt tgtgccaaag ccagatgccc agtgcggcgg
    23761 cggagagggt cagcgaagtg ttgacgatga agctgcacga atgcagcaga gtcgccagcc
    23821 gcatttgggc gcgcaccgta accataaatt cttccatcga ctgcttggca tagacggctt
    23881 cgcgcgcgcc gtggaaaaag agtttgacgg tggcgatatt ggaataggca tcggtaatgc
    23941 ggccggtcat cagcgagcgg gcattcgcct gccgtgcggc ggtttgcccc aatctgggaa
    24001 tcagcaggcg cattaccgaa gcgaaaccga ttatccagcc gataaagggc agcagcagcc
    24061 atgagtcgag cgaggcgaga atcacgcccg aagtgatgaa atacaccgac acataaacga
    24121 ccatatcggc aaccgtcatc accgcgtcgc gcaacgccag cgcggtctgc atgactttgg
    24181 cggacacgcg gccggcaaat tcgtcctgat aaaaaccgag gctctggttc agcatcaggc
    24241 ggtggaagtt ccagcgcagg cgcataggga acacgccctg aagggtttgc aggcgcacgt
    24301 tggacgcggc aaacgcccac acgaccgaaa acaccatcat cgccgccatt gccgccagtt
    24361 cccaaccttt ttcggcaaac agttcggcgg gcgcgtattt gccgagccac tctacgattt
    24421 tgcccataaa ttgaaaaatc agggcttcca taatgccgat gccggcggtc agcgcagcta
    24481 gggcggctat ccatttccgc accccgtcca tactgctcca gacaaaccgg cacaagcctt
    24541 tttcgggcgt tttcggcgcg gcttcggggt aggggtcgat tcgggactcg aaccaggaaa
    24601 atattttgtt caacattgtt ttcgatttcg gtaaaacagt ttcagacggc atcaaacaca
    24661 atgccgtccg aaaggaggga caataacgcc attttacggg aaaagccgtc gggaagacag
    24721 cgcggggcgg aaacgcaggg tttcgtcagg gcaaacgccg cgccgccttc ggacggcatt
    24781 atttcagcag gtttttcagg gcaaggcgca cgccttcgcc cacgtccgtc cccttcggaa
    24841 cgcctttgac cgccgctttt gcctcgcgtt cgttgtaacc cagcgcaagc agcgtgctga
    24901 cgatgtcttc cgtttcgtcg gcggcgggtg aggcggcaaa cagcctgtcc gttaccgtat
    24961 gcgcgaccag cttgccgcgc agttccaaga ccatacgttc ggcggttttt ttgccgattc
    25021 ccggagcgga ggagaggcgt ttgacatctt cttctgcaac cgcccgcgcc agctcgtctg
    25081 ccgtcatcgc cgacaaaatg cccaaagccg ttttcgcgcc gatgccgccg accttaatca
    25141 gttggcggaa ggtcttgcgt tcttccgcag tggcaaaacc aaataaaaga tgtgcgtctt
    25201 cccgaatgat aagctgggta aacagttgta cgctttcacc cacgggcggc aggttgtaga
    25261 aggtctgcat cgatacgtcg gcttcataac cgacaccgtt gacatcgatg acgatttgcg
    25321 gagggttttt ttcaaccagt ttgccggtca gtctgctgat catgtgtgcc gaatcctgaa
    25381 gtgtcgggtg caaaatgccg tctgaaaccg gttcgggctc cagacggcac ggattgtatc
    25441 aaattcagtc gtcgcggcgg gaggaaatca cgcggccggt acgggcatcg acaacgactt
    25501 tgtattcctg tccgtttttg acgatttcga catcatagtg cggacggccg tcgtcgtgtt
    25561 tgagatcgat gtcggtgatt ttgccgccga cacgcgccca agccgctttt tcggcttggg
    25621 cgcggctgat gattttgtct tgtttgccgt gttggtgtgc ggcgtgtccg tggtcgtcat
    25681 cgccgtgtcc gtcgtggtgg gcgagtgcgg gggcggaaat gcccaacagt gcggttgcgg
    25741 cggcggtcaa gagaaggtgt ttgatgttca tatttgccct tgtaaatcag gggttgaaaa
    25801 atggggatat taataaggta tcgaataacc gtcaaccgga ggtcagtacc gcccgaacca
    25861 taccgcgtgc ctgcgcttcg gcttcggcgg cgcgttcctg cgaggcaaac gggcccattt
    25921 tgacaacgta ttcgtaacgg cgtttttcaa ccgagaggtt cgggcttgat gacgaagcgg
    25981 cgaaattttg ggcggcttgg ttcagatagg cttgtgcttc gtgttccgta ccgaaagatt
    26041 tcaagtcgat aaagatgtct ttgttttcgg caaccggtgc ggattggccc gggacgattt
    26101 gttcgatttt gacgtgtgcc gtcccttggc tgacaaagcc caatttttgc gcggcggctt
    26161 tggatacgtc gatgatgcgg ttgccgtgga aggggccgcg gtcgttgacg cggacgatga
    26221 cgcttttgcc gtttttggtg ttggttacgc gcacatggct ggggatgggc agggttttgt
    26281 gggcggcggt aaaggcgttc atatcgtagc ggtctccgcc ggaagttttg cgcccgtgaa
    26341 acctgccgcc gtaccacgag gcgttgccgg tttgcgtgaa ttcggcgact ttgtagctgc
    26401 ggttggcgga ggcgtgcagt ttttccgcct tgaccactgc gtcggcggat gccgtctgaa
    26461 gggagtgtgt gccgaacgcg gcggtgagga ggaaaagggt ttttcgggtt aaagtcaaaa
    26521 tgtgttccgt tcttgagttg aagacggatg ggcattatgc cctacggacg cgttccgaac
    26581 cgccgcacgc tgcggcagcg gttcggaatg tgtccgaacg gttttcagac ggcatgaacc
    26641 tgcgttcaaa cgccgcctgc gtaaccgtgt tgccgccacg cttcaaagag aatcacggag
    26701 acggtgttgg aaaggttcat actccggctg ccgggccgca tcggcaggcg gattttttgc
    26761 gcggcgggca ggctgtcgag gatgtcggca ggcagcccgc gcgtttccgg cccgaacagt
    26821 aaaacgtcgc ctttttgaaa cgcggtttca tcggggcgcg ccgtgccttt ggtggtcagg
    26881 gcgaaaatac gtcggcctgc cagcgacttg aggcagtcgt cgaagttttc gtgcaccgtc
    26941 aggctggcga attcgtggta gtcgagcccg gcgcgtttca ttttggcgga atccaatggg
    27001 aagccgagcg gtttgacaag gtgcaaatcc gcgccggtat tggcgcacag gcggatgatg
    27061 ttgcccgtgt tcggggggat ttccggctga tataaaacga tggtaagcat aaatatcaat
    27121 cacttatagg cgcgtaacct tgccataagg cggatggggt gtcaaaaaat ttagttattt
    27181 tttcattggc gtgcgtgcca gcgtccagca gcagattcgg tttgcgcccg attttttcag
    27241 cgtctttgcc aattcgtcca gcgtcgcgcc ggtggtaaag acatcgtcga ttaacaaaat
    27301 attacagttt tccggtatcg gtgtgcggat ttcaaaggcg tttttgatgt ttcgccgccg
    27361 ttcgccgcct ttgagcgtgc tttgcggcgg gcggtggtgt cggaatacgg tgtgtcgggg
    27421 cagtatctgc cagccgtagc gttgcgccaa caacccgacg atgctctcgc tttggttgaa
    27481 cccgcgttgc agcagccgct ccctgcttag cggaacgggt aggacgaaat cgaaacattc
    27541 gtctgaaagc cggtcgggcg gattctgcat cgtcaggtct gccagcggct gcgccatgcc
    27601 caaatcagcc aagtgcttca gcgcgcgtat catattgctg acgggcggtt cgtaatgcag
    27661 cgatgcccac atccggtcga atgcgggcgg ttttttctga catccgccgc acaccgcgcc
    27721 gccttgaacg tgcctgaaac acaggggaca gctgtttgcc gcgtcggtgc ggaaacttgc
    27781 caaatcggta ttgcagccgg cgcagatgcc gtctgaaacg ccggacgaac cgtggcataa
    27841 tacgcaacgc ctgatagtgg gcgcgtctgc gatgcgccgc caacaagaga gaaaattcat
    27901 gcctgatgct gccaaaaaag tttacctgat acacggttgg ggggcgaacc gccacgcgtt
    27961 cgacgatttg atgccgcgcc tgcccgcaac gtggccggtg tccgccgtcg atttgcccgg
    28021 acacggggac gcgcccttcg cgcaaccctt cgatattgag gcagctgccg acggcattgc
    28081 cgctcaaatc gatacgtcgg ccgacattct cggctggtca ctcggcggat tggtcgcgct
    28141 gtatctggcg gcgcgccatc ccgacaaagt ccgttcgctc tgcctgacgg cgagtttcgc
    28201 acggctgacg gctgccgaag actatcccga agggcttgcc gcgcctgcac tgggcaaaat
    28261 ggtcggcgcg ttccgaacgg attatgccaa acatatcaaa cagtttctac aactccagct
    28321 tctgcacacg cctgatgccg ccgaaatcat cggcagaatc ctgcccgatt tggcgcgctg
    28381 cggcacgcct caagccttac aggaagcttt ggacgcggcg gaaagggcgg atgtgcggca
    28441 tttgttggac aagatagatg ttccggtact gctggtgttc ggcggcaaag acgcgattac
    28501 accgccgcgt atgggcgagt atctgcatcg gcatttgaaa ggaagcaggc tggttgtgat
    28561 ggaaaaggcg gcacacgcgc cgtttttgag ccatgcggaa gtgtttgccg cgctgtaccg
    28621 cgactttgtc gaagggggtt tgacatgaat ccgcaggaca aatgctggca ggttcaccgc
    28681 catcttgccg aacataccga ccaacgcctg acactcgtcc gcaacgcgcc cgggcatatc
    28741 ctgcttgccg gtgcggatgc ggacatcagc cgcagcctgt tggcgaagcg ctatccgctg
    28801 gcggtatttg aagaatacga ttcccgtgcg gattttttgg cggctgccgc agccgcgcgc
    28861 aagggcggtt tctggcaaaa actgacgggc aggggcgtgg tgcagcactg ccaatccccg
    28921 accgtgccgc tgcccgaagc gtgtgccgat atgttgtggt cgaacctcgg actgttggcg
    28981 gcggaacaaa tccttcccgt gctgcacaac tgggcgcgcg cgctgaagac ggacgggctg
    29041 ctgtttttca cctgcttcgg gcgcgatacc ttggcggaac tgaaatcccg tctgaaagaa
    29101 aacggcattg aaagccgcag cgtgatgttt cccgatatgc acgacttggc cgatatgctc
    29161 gccgaaaacg gcttttacga tccagttacc gatacggcga agctggtgtt ggactacaaa
    29221 aaggcggaaa cgttttgggc ggatatggac acgctgggcg tttggcgggc ggtggcgtgg
    29281 gacgacgaaa acgccgcgcg ttcgtgtgcc ggggcgatat ttgaacggga aggcggtttg
    29341 ggcattacgc tggaaacggt gtacggacac gccgtgaaaa aactggtgct gccgcagggg
    29401 gagaacgtgg tgcgtttttt tccgaagaga tgatgtgcag atgacgtctg aagccgtttc
    29461 caagtttcag acggcatttg tctgtgaaaa ccgacagaaa taaaggaaat gccgatgtat
    29521 agtgaattaa atttaaaccg gtacggcgtt ggctcgcctt gccgtactat ctgtactgtc
    29581 cgcggcttcg ccgccttgtc ctgatttaaa tttaatccac tatgtgccga tgccggagac
    29641 gtatattgcg tctataacat cagactgaag cagtacactg cctgccaggt tacccgagtt
    29701 gaagaacacg gtggcaaaaa aacacatgcg accctgctgg ctttggactg gcggggcaac
    29761 aaaccgcttg gggcggcgga gctggcggat ttgaaaccgc tttacaaaga ctttatgtat
    29821 tgggaacgcg gtctgcacat gtataaggca agtgccgtcg tgccgacggg atatgtacgg
    29881 gttgggaata ccgcgccgct gtgcggcgaa gacacgcagc ggtatgcctc tttttggggc
    29941 gacggctacg acgtgtaccg tcagttgaga tggcggcaga tacccgaaaa acagagaaag
    30001 gcattcaaaa aagccgccaa aagcaaaaat accgtgatgt ttgccggacg ggaatacggc
    30061 atatccaaac agaatttgag cgatgtttgg gatgattttg aagacgcgat ggaactgaag
    30121 gcgtttccct gcctgtcttc gctgtttctg accaagtggc ataaaaatct atatgaatat
    30181 ctggaggaat atccgtttat cacccgattg tgtctggaaa accacggtca gaccgtgctg
    30241 gatttcagca atacgcgcat aaccgacctt tctgtcgata tgaccggcgt ggaatcgctt
    30301 tatctgaacg aaggcttgga cagcctgaac ctgaaaggcg aaatcaagga aaactgcaag
    30361 gtctgtaccg ccggaaaggg cgcgggtctg attttggaag tgggcaaaag cgttcccaaa
    30421 gtgcggggtt tggaaaacct gaccgccgtc aacgtcatgg gcattgccga tttcgatatg
    30481 caaaacctgt cggaaacgta tcccaagctc aaaaccatac ggctgtgggg caaaccgggc
    30541 aatatcgcca atttttccgc cgtatcggga tttgaggatt tggaagtgtt cactgccgta
    30601 gatttgttcg gtttcggcgc ggacgacatc cctcatcccg accggctgcc gaagctgcac
    30661 aggctgtgga tgagcagcct gcccgaagag gcggcgaagg cagtgaagaa actttataag
    30721 aaacggaagg aagacgggct ggacctgtgg atagaaaaag cccgaaaacc cgaatggctg
    30781 gcgcaaaatt tcgacaaccc cttccgcgat tgggacggcg cggagcatat cccgaaaagc
    30841 catgccaaaa aagcggcgga gctgtaccgg aaaacccgtg ccggcgtggt caaactgctc
    30901 ggcaatccgc ctgaaaacac aggggagggg ctggcagagg cggtcaaagc ctataccgga
    30961 ggtttcaaca aaatggacaa aaaacacttt atcgacacgg tagagcggga agatattgcc
    31021 gaggcgttgg aaacgatatt ggacctgata ccggatgggt cttgtgccga caaggaaaaa
    31081 ctgtttgaaa tatttgataa aaacaggaat ttttaaacag aggcaatgcc gtctgaaact
    31141 tggtaacggg cttcagacgg catcccgttc caacaccgcc aacaccgccg caccgtaacg
    31201 tgcggctttt tcttcgccta cgccgtatac ggcggcaagc tccgccaaac cttccggctg
    31261 tttggcggca atggcgcgca gggcggcttt gctgagaatg cggtagggtt cggactgttc
    31321 gtgttttgcg gtttcgttgc accattgaat cagggcgcgc atcaggcagc gtttgtgttt
    31381 ggcggtttca tcgatgccgt ctgaaaacgg acggcagacg gcgaggatgt cccgtccgta
    31441 tttggcggcg cgtacgctgc ccaagccgtg tacggcttcg aggtcggttt cggtttcggg
    31501 cgtggcggca agcatatcgg caaggctttc gtcggagagg acggtatgca gggcgcagtt
    31561 ttccgccctt gcctgttcat accgccaggc ttcgagtttt tgacgcagtt gttgttcgcg
    31621 ttcggtttgc ggacggatga cggcatcgtg gctaaagtcg gcagcgtttt ggcagacttt
    31681 gagtatgccg tgtccgaaac gttcggtttt ggcttcgccc aagccgtaga tgtcgtgcag
    31741 cccgttgagg ttttgcggca ttttttcgac aaggtcgcgc agggttttgt cgccgaaaat
    31801 catataggcg gggatgcctt cggcttcagc ctgtttcatg cgccaaacgc gcaatgcctg
    31861 ccacaggcgt tcttcgcgtt cggtacgcag ccagttgtct ttgagggtgc gggtggcggg
    31921 cttgtcgcgc ttgagcgggc gcagcatcac ttcggtttcg cctttgagga cttttttggc
    31981 ggcttcggtc agttgcaatg cctgatatcg ggcaatgttg acggtgaggt agccgaggct
    32041 gatgcactgg cggatgacgc tgcgccattc tttgtcggac aattccgcgc cgatgccgaa
    32101 tgtggacagt tgctcgtgcc ggttgccgcg tatccaatcg tcgcttttgc cgcgcaaaag
    32161 gttggtgatg taaccggcgg caaagcgttg tccggcgcgg tacacgcagc tgagtaattt
    32221 ttgcaccaac accgtgccgt caaaccgtac gggcggatgc aggcagttgt cacaatgtcc
    32281 gcagggttcg gatgcttcgc cgaaatgttt gagcagcagt acgcggcggc aggcggcggt
    32341 ttcgcagacg gaaagcatgg catcgagttt ttgcatttcg atttgctttt gcacctcgtc
    32401 gctgttgcct tcggcaatcc gttcgcgcag caacacccaa tcgttcaagc cgtaacacag
    32461 ccaactcacg gcaggcagcc cgtcccggcc ggcgcgccct gattcctgat agaaatgttc
    32521 gacactctgg ggcatatcga gatgggcgac aaagcgcacg tcgggtttgt ctatgcccat
    32581 gccgaacgcc acggtcgcca ccacgataat attgtcttca tgcgtaaagc ggcgttggtt
    32641 ttcctcgcgc acgtccatgc tcaaaccggc atgatacgga atcgcgttta atccgttttc
    32701 acgcaaaaac tgcgccgcat cttcaacctt tttgcggctt aggcaataca caatgccgct
    32761 ttgccccgtc atttctttgc ggatgaaatc cagcaattgt tttttgccgt tgtttttttc
    32821 gataacctga taataaatat tcgtgcggtc aaagctggag acaaattcgg acgcgtcgtc
    32881 caagtgcaga taatgcttga tgtcggcgcg cgtggcggca tcggcggtgg cggtcagggc
    32941 gatgcgcggg atgttcggat agcgttcggc aagcataccg agctgttgat attcggggcg
    33001 gaaatcgtgt ccccaccggc tgacgcagtg cgcttcgtca atggcgaaca ggctgacggt
    33061 ttgttggtcg agaaaacgta aaaagcggtc ggtaaccaag cgttccggcg cgacataaag
    33121 cagcttcaga cggccttggg caagcttgtc ggcaatctcg cgcgcctcat ctgccgatgt
    33181 gccgctgttg actgccgccg cttcaatccc ggccacatgc aggctggcca cttggtcgtt
    33241 catcagcgca atcagcggcg atacgacaac cgccacgcct tcgcgcatca gcgccggaat
    33301 ctggtaacac aaagacttgc ccccgcccgt cggcatcagc accgtcaaac tgccgccgcc
    33361 tgccaaagta ttgatgacat cctcctgcct gccgcgaaat tcgggatagc cgaatacttc
    33421 gtgcaaaatc tgtctggcgg tgggtcggtg cgtcattgtt ccgtgtccgg taagggtgtt
    33481 ggtcggtcgg cggcaatatg ccgtctgaaa tcgggatttt agaagagttt gcccacttcc
    33541 gccccaatat cgtcggcacg cataaacgtt tcgccgatca ggaaggtatg cacgccgcgc
    33601 gcccgcataa attccacatc cgccttgcct gtaatgccgc tttcggtaac gacggttttg
    33661 ccttccagcg cgggcagcag cgacagggtt tggtcgaggg agacttcaaa agtccgcagg
    33721 ttgcggttgt ttacgccccg caacggcgtg gtcaggttgc ggcatttttc caattcggtt
    33781 tcgtcgtgca gctcgagcag gacggtcatg cccaattcgt gcgccagcgc ctcaaagcgt
    33841 tccaattgtc cctgttccag tgcggcggca atcagcagga cggcatccgc cccccatgcg
    33901 cgcgcctgat aaacctggta ttcgtcgatg atgaagtctt tgcgcagcac gggcagcaat
    33961 acggcttcgc gcgcctgttt gaggtattcg ggcgaacctt ggaaataggg ttcgtcggtc
    34021 agtaccgaca aacacgccgc cccggcgttt tcataggcgc gggcaatctc ggcagggcgg
    34081 aagtcggggc ggattaaccc tttgctcggg cttgcctttt tgatttcggc aatgacggcg
    34141 ggcaggttta ggcggtgttt gccgcgtatc gaatcgatga agctgcggac gggcgcggct
    34201 tctgcggcaa gtgcgcggat gtgttcggcg ttgacggcgg ctttttgggc ggcaacttcc
    34261 tgtgccttgg tggcaaggat tttattgagg atgtcggtca tgtcgtgttc cgtatttgtc
    34321 cggggaaagg gggaatatta gcatcaaacc gttaacgcct gtttgtgcgg aagctgtcaa
    34381 aataggacag gacggtctgc ggcagccatt gcaggtgcag cctgccgccg gtgctgctga
    34441 caaagccggc gtgcccgccg tgtgcaggtt ggaacagggt aacggcttcg gacacttcgt
    34501 ccgcacgggg cagggcttcg ggcggcagga aggggtcgtt ggcggcattg agcaggagca
    34561 gcggtttggc aacgtgtttg agcagcggtt tgcaggaagt ttggcggtag tagtcgtgcc
    34621 ggtcggcaaa gccgtgcagc ggtgcggtga aacggtcgtc aaactcgccc agtgttttgc
    34681 accctgcggc aaatgccgtc tgaaaacctt ggagcgaacg tgctttgggt atcagtgtgc
    34741 ggaggaagta gcgcgtgtag agcagccgcg tgatgccgct gtcgaagcgg ctgcctgccg
    34801 cctctgcatc aacgggggcg gatacggcgg ccgaggcgtg cggcaatgcc tttttgccct
    34861 gttcgcccaa atattttgcc ggcgcgttgc cgcccagcga tacgccgacg gcgtatattt
    34921 cacggtaacg cgcggtgagc gtgtccaaag caaaggcgat ttcggcggta tcacccaagt
    34981 ggtagaacac cggggcggtg ttcgctacgc cgccgcagct gcggaaatgg acgactgcgc
    35041 cgtgccaacc ccgattgcgt accgcgagca tcagttcggc cgcgtaatgg ctgcggctgc
    35101 ttccttccaa accgtgaaac agcacgacca gcggcgcatc gggcgaaatg ccgcctgaaa
    35161 agtcgtaggc ggttttggtt ttacccgtgc tgtcgggaag catctcgcgg cggtatgcgg
    35221 gtgcggggtg ttgcaggaat ttggcggcaa tcgtgtcggc attgccgttg cggaggaaaa
    35281 agggcgtgtc cggcggtgtc aaaatcataa ggtatcggtt tttctgtttt cagacggcat
    35341 tggtgatgcg gcagcccgtc cggccggtgc ggacgcgggc gatgcgcgcc cggatatagg
    35401 cgcggaaaaa gcgttttccg aaaaaggata tcggcatcgg tcagctttcc acgcgtttga
    35461 aatggcgcgg acggaagccc aaagccgcca gagatgcgaa atacagtccg ccgccgacgg
    35521 caatcaggat gcagagctgc cccgctttcc gcattccgcc ggcgtgcgcc cattcgaacg
    35581 gcaggcaagc ctgcgccgcc cacagtccgc cgcacatcac ggcgagcgcg agcagcattt
    35641 tcgccaagaa cgccgcccaa cccctgccgg gccggtaaat accgtgtttg cgcaacagga
    35701 agaacaacaa tccggcgttg atgcacgcgc ccaggccgat ggcgagcgaa agcccggcgt
    35761 gtttcaacgg accgataaag gcgaggttca tcaactgcgt gcagatgagc gtgaagatgg
    35821 cgattttgac gggcgttttg atgttttgcc gcgcataaaa gccggatgcc aacactttga
    35881 tcataattaa accgattaaa ccgaaagaat aggcaatcag cgcgtgttgc gtcatttgtg
    35941 cgtcaaacag cgtgaattct cggtacataa acagcgtcgc caccagcggg aacgacaata
    36001 ccgccagtcc ggccgccgcc ggcagcgtca gcagcatgca caggcgcaaa ccccagtcga
    36061 gcagggcgga aaactgttcc gtatcttggt ttgccgagtg tttggacaaa gtcggcagca
    36121 aaattgtacc gagtgcagcc cccagcacgc cgccgggcag ctccatcatg cggtcggcgt
    36181 aatacatcca tgaaacgctg cccgattgca gataagacgc gaaaatcgtg ttgataacca
    36241 aagaaatttg cgccacgctc acgcccaaaa tcgcaggcgc catctgtttc atgacgcggt
    36301 tgaccgccgc atctttgaaa ttcagtttgg gcagtttcaa aaagcccagt ttcgccagcc
    36361 acggcagttg gaaaccgagc tgcaaaatac cgccgacaaa aaccgcccac gccagcgcgg
    36421 taacgggcgg atcgaaatac ggcacgaaaa acagtgcgaa tacgataaaa gagatgttta
    36481 aaaacgtggg cgtaaacgcg ggaatgccga acttatggta ggaattgagt atcgagccga
    36541 caaaagaaga caaagaaatc aataatatat aaggaaacgt aatccgcagc aggctgatgg
    36601 aaagttggaa cttgtccgcg tctttggtaa agccgggcgc ggaaacataa atcacccaag
    36661 gcgcggcaag tatgcccagc gcggtaacga cgatcagcac aaacgacagc attcccgcaa
    36721 cgtggcggat aaaagcctcc gtcgcctctt tagaacgcgt ttccttatat tccgccaaaa
    36781 tcggcacaaa cgcttgggca aacgccccct ccgcaaacac gcggcgaagc aggttgggca
    36841 gtttgaacgc gacaaaaaac gcatccgtcg ccatacccgc gccgaatgcc cgcgcaatga
    36901 ccgtatcgcg cacaaatccc aaaacgcgcg acaccatcgt caggctgccg acttttgcca
    36961 aagctccaag catattcatc gtgtttcctt ttcggttgaa accccgccct ttagggcggc
    37021 ag
//
LOCUS       DAXDZG010000012        36896 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXDZG010000012 DAXDZG010000000
VERSION     DAXDZG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36896
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.012"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            71..2208
                     /gene="tdfF"
                     /locus_tag="ACFB78_000511"
                     /pseudo
     CDS             71..2208
                     /gene="tdfF"
                     /locus_tag="ACFB78_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
     gene            complement(2471..2794)
                     /locus_tag="ACFB78_000512"
     CDS             complement(2471..2794)
                     /locus_tag="ACFB78_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC1268578.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(2875..3531)
                     /locus_tag="ACFB78_000513"
     CDS             complement(2875..3531)
                     /locus_tag="ACFB78_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC1268579.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(3634..4161)
                     /locus_tag="ACFB78_000514"
     CDS             complement(3634..4161)
                     /locus_tag="ACFB78_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC1268580.1"
                     /translation="MYANMGDMMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWLWVGFYFVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGEL
                     AKILERRFEASRQAA"
     gene            4320..5093
                     /gene="tpiA"
                     /locus_tag="ACFB78_000515"
     CDS             4320..5093
                     /gene="tpiA"
                     /locus_tag="ACFB78_000515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC1268581.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5100..5450
                     /gene="secG"
                     /locus_tag="ACFB78_000516"
     CDS             5100..5450
                     /gene="secG"
                     /locus_tag="ACFB78_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC1268582.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            5464..5549
                     /locus_tag="ACFB78_000517"
     tRNA            5464..5549
                     /locus_tag="ACFB78_000517"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5498..5500,aa:Leu,seq:gag)
     gene            5932..6053
                     /locus_tag="ACFB78_000518"
                     /pseudo
     CDS             5932..6053
                     /locus_tag="ACFB78_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6439..6806
                     /locus_tag="ACFB78_000519"
                     /pseudo
     CDS             6439..6806
                     /locus_tag="ACFB78_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6803..7114
                     /locus_tag="ACFB78_000520"
     CDS             6803..7114
                     /locus_tag="ACFB78_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1268583.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            7224..7424
                     /locus_tag="ACFB78_000521"
     CDS             7224..7424
                     /locus_tag="ACFB78_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268584.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPVARHGDFLRP
                     LQGGLDLSGTGLKAKRAGRRTV"
     gene            complement(7885..8440)
                     /locus_tag="ACFB78_000522"
                     /pseudo
     CDS             complement(7885..8440)
                     /locus_tag="ACFB78_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8437..9245)
                     /locus_tag="ACFB78_000523"
                     /pseudo
     CDS             complement(8437..9245)
                     /locus_tag="ACFB78_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(9238..9408)
                     /locus_tag="ACFB78_000524"
                     /pseudo
     CDS             complement(9238..9408)
                     /locus_tag="ACFB78_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(9375..10166)
                     /locus_tag="ACFB78_000525"
     CDS             complement(9375..10166)
                     /locus_tag="ACFB78_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC1268585.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(10395..13025)
                     /gene="leuS"
                     /locus_tag="ACFB78_000526"
     CDS             complement(10395..13025)
                     /gene="leuS"
                     /locus_tag="ACFB78_000526"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC1268586.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            13292..13808
                     /locus_tag="ACFB78_000527"
                     /pseudo
     CDS             13292..13808
                     /locus_tag="ACFB78_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            13815..14258
                     /locus_tag="ACFB78_000528"
     CDS             13815..14258
                     /locus_tag="ACFB78_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC1268587.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            14427..16484
                     /gene="ppk1"
                     /locus_tag="ACFB78_000529"
     CDS             14427..16484
                     /gene="ppk1"
                     /locus_tag="ACFB78_000529"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC1268588.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(16633..17736)
                     /gene="dnaN"
                     /locus_tag="ACFB78_000530"
     CDS             complement(16633..17736)
                     /gene="dnaN"
                     /locus_tag="ACFB78_000530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC1268589.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(17816..19372)
                     /gene="dnaA"
                     /locus_tag="ACFB78_000531"
     CDS             complement(17816..19372)
                     /gene="dnaA"
                     /locus_tag="ACFB78_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC1268590.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            19664..19798
                     /gene="rpmH"
                     /locus_tag="ACFB78_000532"
     CDS             19664..19798
                     /gene="rpmH"
                     /locus_tag="ACFB78_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC1268591.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            19801..20166
                     /gene="rnpA"
                     /locus_tag="ACFB78_000533"
     CDS             19801..20166
                     /gene="rnpA"
                     /locus_tag="ACFB78_000533"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC1268592.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            20231..20452
                     /gene="yidD"
                     /locus_tag="ACFB78_000534"
     CDS             20231..20452
                     /gene="yidD"
                     /locus_tag="ACFB78_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC1268593.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            20625..22262
                     /gene="yidC"
                     /locus_tag="ACFB78_000535"
     CDS             20625..22262
                     /gene="yidC"
                     /locus_tag="ACFB78_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC1268594.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(22343..23053)
                     /locus_tag="ACFB78_000536"
     CDS             complement(22343..23053)
                     /locus_tag="ACFB78_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC1268595.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(23115..23705)
                     /locus_tag="ACFB78_000537"
     CDS             complement(23115..23705)
                     /locus_tag="ACFB78_000537"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC1268596.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            23853..24353
                     /locus_tag="ACFB78_000538"
     CDS             23853..24353
                     /locus_tag="ACFB78_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC1268597.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            24387..24566
                     /gene="rpmF"
                     /locus_tag="ACFB78_000539"
     CDS             24387..24566
                     /gene="rpmF"
                     /locus_tag="ACFB78_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC1268598.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            24741..25268
                     /locus_tag="ACFB78_000540"
     CDS             24741..25268
                     /locus_tag="ACFB78_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC1268599.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            25358..26404
                     /gene="plsX"
                     /locus_tag="ACFB78_000541"
     CDS             25358..26404
                     /gene="plsX"
                     /locus_tag="ACFB78_000541"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC1268600.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            26542..26793
                     /locus_tag="ACFB78_000542"
     CDS             26542..26793
                     /locus_tag="ACFB78_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC1268601.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            26820..27038
                     /locus_tag="ACFB78_000543"
                     /pseudo
     CDS             26820..27038
                     /locus_tag="ACFB78_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27189..27380
                     /locus_tag="ACFB78_000544"
                     /pseudo
     CDS             27189..27380
                     /locus_tag="ACFB78_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27410..27613
                     /locus_tag="ACFB78_000545"
     CDS             27410..27613
                     /locus_tag="ACFB78_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268602.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            27640..28602
                     /locus_tag="ACFB78_000546"
     CDS             27640..28602
                     /locus_tag="ACFB78_000546"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC1268603.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            28649..29020
                     /locus_tag="ACFB78_000547"
     CDS             28649..29020
                     /locus_tag="ACFB78_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC1268604.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            29046..29972
                     /gene="fabD"
                     /locus_tag="ACFB78_000548"
     CDS             29046..29972
                     /gene="fabD"
                     /locus_tag="ACFB78_000548"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC1268605.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            30111..31976
                     /gene="msbA"
                     /locus_tag="ACFB78_000549"
     CDS             30111..31976
                     /gene="msbA"
                     /locus_tag="ACFB78_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC1268606.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGILSTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            32076..33641
                     /gene="guaA"
                     /locus_tag="ACFB78_000550"
     CDS             32076..33641
                     /gene="guaA"
                     /locus_tag="ACFB78_000550"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC1268607.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            33703..34449
                     /gene="fabG"
                     /locus_tag="ACFB78_000551"
     CDS             33703..34449
                     /gene="fabG"
                     /locus_tag="ACFB78_000551"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC1268608.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            34514..34923
                     /locus_tag="ACFB78_000552"
                     /pseudo
     CDS             34514..34923
                     /locus_tag="ACFB78_000552"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            34921..34998
                     /locus_tag="ACFB78_000553"
     CDS             34921..34998
                     /locus_tag="ACFB78_000553"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFC1268609.1"
                     /translation="MKQPCICGSGKKFKACCGKYLQPVA"
     gene            complement(35218..36012)
                     /locus_tag="ACFB78_000554"
     CDS             complement(35218..36012)
                     /locus_tag="ACFB78_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC1268610.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            36116..36841
                     /locus_tag="ACFB78_000555"
     CDS             36116..36841
                     /locus_tag="ACFB78_000555"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC1268611.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSENTRLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
BASE COUNT         9188 a        11059 c         8887 g         7762 t
ORIGIN      
        1 caacgccgta ccggtttaaa gttaatccgc tatacaaccc caccgcacac caacacacaa
       61 aggaaacccc atgacacgct tcaaatactc cctgcttttt gccgccctgc tacccgtgta
      121 cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca ccgaattgcc
      181 gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca ccgtttccgg
      241 cacgcacacc ccgttcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt
      301 catcacatcg caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca
      361 ggcgaccggc accagcccgg ctccgaccgc gcgggctaca actacctgtt cgcgcgcggc
      421 agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct ggccgatacg
      481 ggcaatgcca acaccgccgc ctatgagcgc gtggaagtcg tgcgcggcgt ggcggggctg
      541 ccggacggca cgggcgagcc ttctgccacc gtcaatctgg tacgcaaaca cccgacccgc
      601 aagccattgt ttgaagtccg cgccgaagcc ggcaaccgca aacatttcgg gctgggcgcg
      661 gacgtatcgg gcagcctgaa cgccgaaggc acgctgcgcg gccgcctggt ttccaccttc
      721 ggacgcggcg actcgtggcg gcagctcgaa cgcagccgcg atgccgaact ctacggcatt
      781 ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta ccagcaggcg
      841 aaagaaaccg cagacgcgcc gctcagctac gccgtgtacg acagccaagg ttatgccacc
      901 gccttcggcc caaaagacaa ccccgccaca aattggtcga acagccgcaa ccgtgcgctc
      961 aacctgttcg ccggcataga acaccgcttc aaccaagact ggaaactcaa agccgaatac
     1021 gactacaccc gtagccgctt ccgccagccc tacggtgtgg caggcgtact ttccatcgac
     1081 cacagcactg ccgccaccga cctgattccc ggttattggc acgccgatcc gcgcacccac
     1141 agcgccagca tgtcattgac cggcaaatac cgcctgttcg gccgcgagca cgatttaatc
     1201 gcgggtatca acggctacaa atacgccagc aacaaatacg gcgaacgcag catcattccc
     1261 aacgccattc ccaacgccta cgaattttcc cgcacgggcg cctatccgca gccatcatcg
     1321 tttgcccaaa ccatcccgca atacgacacc aggcggcaaa tcggcggcta tctcgccacc
     1381 cgtttccgcg ccgccgacaa cctttcgctg atactcggcg gcagatacag ccgctaccgc
     1441 gcaggcagct acaacagccg cacacaaggc atgacctatg tgtccgccaa ccgtttcacc
     1501 ccctacacag gcatcgtgtt cgatctgacc ggcaacctgt cgctttacgg ctcgtacagc
     1561 agcctgttcg tcccgcaatt gcaaaaagac gaacacggca gctacctgaa acccgtaacc
     1621 ggcaacaatc tggaagccga catcaaaggc gaatggcttg aagggcgtct gaacgcatcc
     1681 gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg cgaccagagc
     1741 ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga aatcgaagtc
     1801 ggcggccgca tcacgcccga atggcagata caggcaggct acagccaaag caaaccccgc
     1861 gaccaagacg gcagccgccc gaaccccgac agcgtacccg aacgcagctt caaactcttc
     1921 accgcctacc acttagcccc cgaagccccc agcggccgga ccatcggtgc gggtgtgcgc
     1981 cggcagggcg aaacccacac cgacccagcc gcgctccaca tccccaatcc cgccgccaaa
     2041 gcccgcgccg tcgccgacat catggcgcgt taccgcttca atccgcgcac cgaactgtcg
     2101 ctgaacgtgg acaacctgtt caacaaacac taccgcaccc agcccgaccg ccacagctac
     2161 ggcgcactgc ggacagtgaa cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa
     2221 acggagtttc tgcgaagcta aaagcagcct gcacattgaa aatgccgccc aagcaaacct
     2281 gcagtttgcc gccctctccc tagccttctc ccacggggcg agggaattgg ggtgcaggct
     2341 gcctttaagg ttcaggcaaa ttttaacttc gttgatactg cgctttagct tcgcagaagc
     2401 tgcactttca gacgaccttt atggaacacc agaggtacac gcatttaagg aatgccgtct
     2461 gaagccgcct tcaataccgc gccatttccg cgtcaacctc aaccgcccac gcgtcgatgc
     2521 cgccctgaag gttgtacagg ttttcaaaac ccgcctccgc caaatacatc gccgtatgca
     2581 ggctgcggat accgtgatgg caatacacca caagcggcac atcgtccggc aactcgtttt
     2641 gccgcagcgg aatcagattc atcgggatat gcagcgcatt tggcagcgaa caaaccgccg
     2701 cttcttcatc cgtacgcacg tccaacaaac aaaacatccg cccttcgtcc atccacgcct
     2761 tcaatgccgc cgacggaagt tgcacaatat ccatcacaaa ccccccgaaa aaaaccaagc
     2821 aaaatgccgt ctgaagcccc aaacccgctt tcagacggca tcacaatccg aacatcaaaa
     2881 attaaaactg tcgaaaggat gggcatcctt gtcgtccaaa tgcgccacca aagtatcgaa
     2941 cagcaccttc tcttcaaaca catcgcccct gcgcgtaatc aaaagcgcgc gctgcaccgg
     3001 cctgcgtccc acaataaccg ccatacgccc gccgtccttc aactgttcct ttaacacttc
     3061 aggcacaagg gttaccgcgc cgccgacgta aaccgcatca aacggcgcac ctgcggaaag
     3121 ttcggtcaac ccgttatttt gcacataatc gatgttttcc aaagacaagc cgtccaaaac
     3181 cgctttggcg cgcttttgcc gttcggcatc gatgtcgtcc gaaaccacac ggccagccag
     3241 ttttgccaac agagccgtcg catagcccga acccgtgccg atttccaaaa ccgtatcgtt
     3301 tttcgtcagc ttcaagccct gcgccagccg cgccacgact ttcggctcga gcatcttatg
     3361 accgttggca agcggcagct ccatatccgc atacgccaaa ccctgcaaag actcatccgc
     3421 gaaaagctcg cgcggaatct cctccaaagc gtccaacacg tcaaaatcca atacatccca
     3481 aggacggatc tgctgttcga ccatattgaa ccgcgctttt tcaaaatcca tttagtgctc
     3541 cgtcaaataa ttgttttatc aatggcaggc attataaaac cacactaccg ccgcgccaac
     3601 ttcggcacgc gaagcccgcc cgttttgcca gtctcaagcc gcctgacgcg aagcctcaaa
     3661 ccgcctctcc aaaatcttcg ccagttcgcc caaatacaaa gcatctgcct catcaaactg
     3721 cgccaaatgt tcgctgtccg cgtccaacac gccgatacag cggccgtctg aaaacagcgg
     3781 cacgacaatc tccgaacgcg acaaagacga acaggcaata tggtcgggat gcgcgttcac
     3841 atccttaaca accaccgttt cacccttcgc ccaagcctga ccgcacaccc cgcgcccgaa
     3901 cggaatccgc gtacacgcca aaggtccctg aaacggtgcc aaaaccaatt cgtccgaacg
     3961 cgtatcgaca aaataaaaac ccacccacaa ccagccgaac gcctccttca aaaccgccgc
     4021 cgtgttcgcc aaattcgcca cccaatccgc ctcgtccgcc accacagact caatctgcgg
     4081 caacacctca cgataaagcg cggccttgtc cgaagccgaa aaatgaagcg cgtgcatcat
     4141 atctcccata tttgcataca tatcaggcag ccattataaa acatcctgcc cgaaacaaca
     4201 ttccaaaccg cccccgcagc cgcccgcccc aagttgcgaa cccgccgcat atccactaaa
     4261 cttcacattg caacgcgcca cacgcggcag acagaaaaac acgacacgga gcaaaaaaga
     4321 tgtatcgcca aatcggaatg tgggatcaaa aatgggtcat cggcaactgg aaaatgaacg
     4381 gccggctcca aaacaacaac gcactgatgc accgcttccg catcctcccc accgccgaac
     4441 gcgtcctcat cggactcgcc gccccgaccg tttacctgct gcaactgcac aacgccatgc
     4501 aaatcgtctt aaacaaccgc atcctcacct gcgcccaaga cgtgagccgc ttccccgaca
     4561 acggcgcgta caccggcgaa gtgtccgccg aaatgctcgc cgacaccggc acagacatcg
     4621 tcctcatcgg acactccgaa cgcagccttt atttcggcga aaaaaacgaa atccaacgcc
     4681 gcaaaatgga aaacgtcctc aacgtcggac tcatcccatt attgtgcgtc ggcgaaagcc
     4741 tcgaagagcg cgaaaccggc aaagaacacg aagtcatcgc ccatcagctt tccatcctgc
     4801 aagggctgga taccaaaaac atcgccgtcg cctacgaacc cgtctgggca atcggcacag
     4861 gcaaagtcgc caccgtcgaa cagattgccg atatgcacgc attcatctac aaagaaatct
     4921 tgtctttgtg cggaagcgat gttaaaatcc gcgtccttta cggcggaagt gtgaaagcgg
     4981 acaacgcggc cgacatcttc gcagtacctt atgtggacgg cgcactcgtc ggcggcgcgt
     5041 cattgtcgta cgactccttt accgccatca tcagtgccgc acaaaatgcg tagaaaaata
     5101 tggaagcctt caaaacccta atttggatta ttaatattat ttccgctttg gccgtcatcg
     5161 tgttagtatt gctccaacac ggcaaaggcg cggatgccgg cgcgaccttc ggatcgggaa
     5221 gcggcagcgc gcaaggcgta ttcggctctg ccggcaacgc caacttcctc agccgctcga
     5281 ccgccgttgc agcaacattt ttcttcgcaa cctgcatggc tatggtgtat attcacaccc
     5341 acacgacaaa acacggtttg gacttcagca acatacgaca gactcagcaa gcacccaaac
     5401 ccgtaagcaa taccgaacct tctgcccctg ttcctcagca gcagaaataa cagtttttca
     5461 aatgccgaca tggtgaaatt ggtagacacg ctatcttgag ggggtagtgg ccgtaggctg
     5521 tgcgagttca aatctcgctg tcggcaccaa cacacaaaaa cgcttagaaa ttctaggcgt
     5581 tttttttcgt ttaatcctgt ctgatgccat caaccaatcc caatcaatac aaggatttca
     5641 ggctgattgt tatcctgccg tccccttcct gactgtgcaa tcccgtccaa tccgccctaa
     5701 ttgaactaac ctgatttttt acggtatccg aaaaataccg caaaaataaa gctgaacgac
     5761 caccaaatca ggaatgccaa gcggaaaaga gcttgcgggg aatactgacg aaccgtaggg
     5821 aacgaggggg aaaccgtcca agatgcagag cggttttttt gggtttttgg aaaaaaccta
     5881 tactaggaag cgataccctt agttgttacc ttgttaccgg ggaaaagtta gataaataag
     5941 tatatgaaat atagtgaatt aaatttaaat caggacaagg cggcgaaccg cagacagtac
     6001 aaatagtacg gcaaggcgag gcaacgccgt accggtttaa atttaattca ctataaaaat
     6061 aagaaaaaga taaaaaattg gtaacaaatg cggtaacaaa tggtaacgaa tcggtaacaa
     6121 cttttggggt tttccggttt tttaccgtct tggcagtggg agcgtagcgg aatgaaaagc
     6181 caaaacgcac ggaaccgcgc ctattttgag caggaatggc ggttaaaccg gttggttata
     6241 ttgcggggca taggaagaca gcgaaacgcg ctaaaaacgc aatttgagcc attaaaagcg
     6301 attgattaaa aaataatcaa attagccgcc ggtgtttcag gcggcataaa cggagaaatt
     6361 gcagggtata aaaaggcggc ttgccgtgtt gcctgtcttt ggtataatcc ccagtatcac
     6421 taagtaaacg gttgcacaat gcggatattc aaaaaccaat ggatagtgaa atttgccaag
     6481 aagcacaaaa tcagtgattc agagctgata gaggcggtag agcgggcaga taacgggctg
     6541 atagatgcag atttgggcgg cgcgtgatta agcagcgtat agcaaggcaa gggcaaggca
     6601 gaagcggcgg ttatcgcagt ctgatatttt tcaggcatgg agaacgggcg tttttcatga
     6661 ccgcctttgc caagaacgac cgcgaaaaca tcactgataa agaacttaca gaattaaaga
     6721 aaaccgccgc catcatcttg gcaatgacag aaaccgacat agagcaagcg aagtcaaacg
     6781 gcactttcac ggagatacag ccatgaaata taaaagcggg attttcgcag caatacacga
     6841 aaccatgcag ggattgcacg acatcggcgc aatcgacaaa aagactatgc gcggctttga
     6901 caagtcctgc ctgaccgaaa tcaagccgtt gagcggcggg gacatcaagg caatcaggga
     6961 gaaggaggca ctatcgcaag ccgctttcgc catctatctc aacgtgggga aaaatcacgt
     7021 ttcggcttgg gagcggggcg ttaaaaagcc gagcggcgcg gcgttgaagc tgctgaccat
     7081 cgtcaaaaac aagggcatcg aagccattgc gtagccgact tggcaaacgg caaaatcagc
     7141 aagttcacaa tagacgcgct gctgaatatg cctgccaaaa caggcaagac cgccgaactg
     7201 aatatcaggg cgtagccgca taaatgcccg accgcatcaa accaagccga aacggcggcg
     7261 gtgcagacgg catagcccga cagcaaggca cggcgcagac gggcgcgaaa cccgaaacat
     7321 ccccggtcgc aaggcacggg gattttttgc gcccgttgca ggggggattg gatttaagcg
     7381 gcacgggctt gaaggcaaaa cgggcggggc gcagaactgt ttaaatgccg tctgaatctc
     7441 aaaccatttc agacggcatt ttgaaacaac ggctcaaatt ctcgatcccc ttcccttaac
     7501 gccgacgttt tttattaacg cgccccttat ttctgacact ttgctcataa accggcataa
     7561 cggtcggcaa caaccgtttt agattttcta tacgggcatt gtttacagca tcagcataag
     7621 acaccgtact ctggaatacc gcctaaaccc tgcggatagt tttggatgtg gtatcgacat
     7681 tgtcggcaga cttgtctaac tcgaccgttt tcaaataatc tttgacagat gctgcattca
     7741 gtgtcgcgga atccgcgtat tttgaacaaa aggtcgggct actttatcac aatagcagaa
     7801 agatggaaaa cggcggggat tggtgaaagg tcgtctgaaa tgatattcag acgaccttga
     7861 acggttgtgt tttaaatatg gtatttattc atttgtttca ccatcaggca gtccaaacca
     7921 ccttaaggga atcgtgttaa attggtaatg gcagttaaca ctgataatat aatctaaaac
     7981 atctttaata ttccggtttt gtaaaccaat cattcaaaac atagacatat ccaacccgcc
     8041 aacctaaaga gtgaactaac tttgtatatt gttttctctt aaaatcgcaa gtttgtaatt
     8101 tctcatctac tgaaccggga gtttgttgga atttgatctc aataataaat aaggtatcac
     8161 ggatgatgat aaataagcca ttatcgggca gtaatctttt agacagatgg tctttccaat
     8221 taatattgta ttccagtctt tccaaaaatc ggtaaaactc atattttttg aaacaataag
     8281 ccttcaattc tccattaaac caaacttcat aacccgcatt atcatctgtt tttcttaagt
     8341 cgtacccggg aatttcttca aacaactttc gtaagtctgt acgttcttcg aatctgagtc
     8401 ctgtttgtgt ccttgcaccg ccgattccgc caataatcat ttttctaacc ttttctttgc
     8461 taattcaaaa aaatcttctt ccagttcgca accgcaaaat ctccgaccat gttttaaggc
     8521 ggcaacgcct gttgtgccgc tgcccataaa agggtcaaaa attaaatctc cgatatttga
     8581 agccgatagt atgcagcgtt caagtaaggg gagtggtttt tgtgtcggat gtttgccgaa
     8641 tgttttttcg gttttatttg gtggaggaaa tgtccaaaca catttcatct gtttgccatc
     8701 attttgtgtt ttcatggttt cataatgaaa cgtgtgttta gcttttttat ttttctttgc
     8761 ctataagatt gtttctgtcg aatgggtaaa gaaacggcag gacaaattag ggggaggatt
     8821 gggtttttcc caagtaatat tgttcaaaat atggtagccg acggtttgca tcaggtagcc
     8881 gattaaatag atattatgaa atgtgccgca aacccaaatt gtgccgtttg gttttaataa
     8941 ggcgtaacac agtcggagcc attcttcgta aaattccaaa tctgccgcca ttcctttgga
     9001 tttatcccag ttgcccttat tgacggaaac catttgcccg ttttgacaac tgaaaccgtc
     9061 attggaaaga aagtaaggcg ggtctgcgaa aatcatatca aaacagccgt ttggatgttt
     9121 ttccaatatc ttccgcatga cgttaaggga attttcgttg tagaggacaa ggtcgtctga
     9181 aacgattttt tcaaacgact ttgataaatg gcagcttctt cagccggttt gtcaaaatca
     9241 atcataaatt gccaataatt ctccgacttt tttacgactg ctgccctttg cggcaatatt
     9301 gcgttgtact tcaaccgttt ttaaaatggc agaagaataa atatttctcg taaattcagt
     9361 atcatgattt gaaatcaaaa cgccttggga gttgggtgca atttgtcgat ttttttgtaa
     9421 agtccgcgac caatgaattc gatcatattt tgatcacgca aaatttgcaa ctgttggcgg
     9481 attttgtctt tgatatggtt gttttgggga aattggatgg atagtttgtt ttcaaattca
     9541 tacatttgcg acaatgtgaa ttcttcggtg agttggtcga tacatttcat aacagccaaa
     9601 agccagtctt tgcgctccgc attttggttg cgtaaaaaca ggttggattg ccattttttc
     9661 agaacggttt cgggttcgat aatgcgggaa tcgtctatta agaatatttt tccgctttca
     9721 ggcaaagggg cgagattgat ggaacacata atgtggtgcg gtcggttttt aatgccttta
     9781 tttctgggaa taatcatatc cggcgtgatg aaatgtttgg gtacaagcac caattgccgt
     9841 atggagtaat ccgctttttt atatgcaaga aagaaaaagt tggggttggt atctgaccgg
     9901 atgcgctcca acatagtgcg atatgcaccg tcaggcacgc tgttgcctat ggttttttga
     9961 tttttactct ttaattcata ttgctcgtgg caatttgggc aaaagaggtc tgcaacaggt
    10021 ttgttattgg caaatctctg catcggcttg cttccgcaac aggggcaata gccgtttttt
    10081 tccagccaag cctcgctcat tacacggatt ttgtgggttg ctttattttg ttgctttccc
    10141 aattgggtat cgaaaaataa attcatattt tggattttga gattaatttt tagtagatta
    10201 acaaaaatca ggacaaggag acgaagccgc agacagcagg gcaaggcggg gcaacgccgt
    10261 accggtttaa agttaatccg ctatatttga ggtctgtctt gcagacaacc caatctgcct
    10321 taaaaaggcc gtctgaaacc ctgtttccaa gtttcagacg gcctttatcc gtatggctaa
    10381 accttaaaag cggtttagac gacgatgttc accagtctgc ccggtacgac gatgattttc
    10441 ttcgcaggct tgccttccat gaatttcact gcgccttcgt tggcgagtgc ggcggcttcg
    10501 aggtcggctt tggaggcgtc ggcggcgacg gtgattttgc cgcgcagttt gccgttgact
    10561 tgaaccatca cttcgatttc ggatttgacc agggcggctt cgtcgactgt cggccagcct
    10621 gcttcccaca gtttcgcgcc gttcaattcg ctccacaggg tttcgcagat gtgcggcacg
    10681 atgggccaca acaggcgtac ggcggcttcc aatacttctt gggcgacggc gcggccttgt
    10741 tcgctgccgg tgtcggtttt gtcgtattgg ttgagcagtt ccatcacggc ggcgatggcg
    10801 gtgttgaact gctggcggcg gccgtagtcg tcgctgactt tggcggtggt ggaatgcagt
    10861 ttgtggcgca ggtctttgag ttctttagac aaaccgtctt ggttgcctgc aaatgctttg
    10921 accgcgccgc cttgcttcag gtattcgtaa acggtacgcc acagacggcg caggaagcgg
    10981 tgtgcacctt cgacgccgct gtcgctccat tcgagggact gttcgggcgg tgcggcgaac
    11041 atcatgaaca ggcgggcggt gtccgcgccg taggcgttaa tcagttcttg cggatcgacg
    11101 ccgttgtttt tggatttgga cattttttcc gtgccgctga tgacgacggg cagtccgtcg
    11161 gctttgagga cggcggaaac ggggcggcct ttgtcatcga aagtcagctc gacatcggcg
    11221 gggttgatcc agtctttgcc gcctttgtcg ttttcgcggt agtaggtttc gcagacgacc
    11281 ataccttgcg tgagcaggcg ttcaaacggt tcgtcaacat tgaccaaacc ttcgtcgcgc
    11341 atcagtttgg tgaagaagcg cgcgtacagg aggtgcaaaa tcgcgtgttc gatgccgccg
    11401 atgtattggt cgaccgcgcc ccagtatttc gcggattctg ccgataccat gccgtctgaa
    11461 aacttgggcg acatatagcg gaagaagtac cagctcgact ccatgaaggt gtccatggtg
    11521 tcggtttcgc gtttcgccgc gccgccgcag cacgggcagg aagtttcgta aaactcgggc
    11581 atttttgcca gcggcgaacc cataccgtcg ggtacgacgt tttcaggcag gacgacgggc
    11641 agttggtcgg cagggacggg aacgtctccg catttttcgc aatggacgat gggaatcggg
    11701 cagccccagt agcgttggcg cgaaatgccc cagtcgcgca ggcggtattg ggttttcggt
    11761 tcgcccgcac cttggctttg cagcttggcg gcaacggcat caaatgccgt ctgaaaatcc
    11821 aagccgtcca agtcgccgct gttgaccaat acgccgtttt ctttgtcgcc gtaccattct
    11881 tgccatcggt ttgcgtcgaa tgcgttgtcg ccgacggcaa tgacttgttt tttcggcaga
    11941 ttgtatttgg cggcaaactc gaaatcgcgt tcgtcgtgcg ccggaacagc catcaccgcg
    12001 ccgtcgccgt agccccacaa gacatagttg gcaatccaca cttccagctt gtcgccgttg
    12061 agcgggttga cgacgtagcg gccggtcggc acgccttttt tctccatcgt cgccatatcg
    12121 gcttcggcaa ccgaaccggc tttgcattcg gcgataaatg cctgcaattc gggtttgtcg
    12181 gcggctgcgg ctgttgccag cggatgttcg gcggcaacag cgacataagt cgcacccatc
    12241 agcgtgtcgg ggcgggtggt ataaacttgc aggaatttcg cgtaatcgcc ttccaaacct
    12301 tgcttgctgt cgtctgaaac ggcgaagcgc acggtcatgc cgcgagattt gccgatccag
    12361 ttgcgctgca tggttttgac ttgttccggc cagtgttcca gcttgtccaa atcgttgagc
    12421 agctcttcgg cgtaatccgt aattttgaag taatacatcg ggatttcgcg tttttcgatc
    12481 aacgcgcccg aacgccagcc gcgtccgtcg atgacttgct cgttggcaag gacggtttgg
    12541 tcgaccgggt cccagttcac cgttccgttt ttgcgataga cgatgccttt ttcaaacagc
    12601 ttggtaaaca gccattgttc ccagcggtag tattcgggtt tgcaggtggc gacttcgcgt
    12661 tcccagtcaa ccgcaaaacc caggcttttg agctgggttt tcatgtattc gatgttgtcg
    12721 taggtccaag cggcgggggc gacgttgttt ttcatcgccg cattttccgc cggcatgccg
    12781 aacgcgtccc aacccatagg ctgcataacg ttgaagccgt ttaaaagttt gaagcggctc
    12841 aatacgtcgc cgatggtgta gttgcgtaca tgccccatat gcagcttgcc gctagggtaa
    12901 gggaacatcg aaaggcaata atatttgggt ttggaagcgt cttcggagac gttggaaata
    12961 cgggcgtcgt cccatttttt ctgcgccgca ggctcgatgg cggcgggctg gtaatgttct
    13021 tgcatggtca ttctgttttt cgcttgaaaa cgttaggaat ttaaaggagg aattataaca
    13081 ggttgccgat gtcggcgaat cggcaaattg ccggcaggat gcgtaaattc gcacgcgcat
    13141 cattccgtat gccgtacaaa tacaccgcgt ttactgatac gcacgttttt tatgctaata
    13201 ttacgaaaca aaatcaaacg tttaaaactc tcctgatgcg gctcttccga acaaaaggca
    13261 gacgggcatc gggtaaaaaa ggattctgca tatgaaaatc aaacaaatcg tcaaaccgag
    13321 cttggcagta ttggcggcag gcattctgtc tgcctgcgca accataaagc aacgtcaaag
    13381 ccgacggcac gaccgacaat ccggttttcc cggaacccta ttccgtaacg ctcgacaaca
    13441 agcgcggcac attcccgact tatgacgaac tggatcagat gcgccccggt ctgaccaaag
    13501 acgacatcta caaaatcccg ggccgcccgc attacgacga aggtatgtac ggcgtgcgcg
    13561 aatgggatta cctgttccac ttccacaccc cgggcgtggg tatcgaccct gaaaacactt
    13621 ccggcgtgga aggcgttact acctgccaat acaaagtgat tttcgataaa gacaaatttg
    13681 cccgcagctt ctactggaac cccgtcttcc cgaaagatgc cgtctgcccg ccgcccgcac
    13741 ccaaagccga gccgcaagtc atcatccgcg aaatcgtgcc ggcaaaaccg aaacgtatcc
    13801 gccaataagc cgctatgccg ccccgcctgc tttcagggat attgtgctgc ctgctgacga
    13861 ctgcccccgt attcgcacag gggcagccgg atgcggtcag tgcctatatt caaaagaaaa
    13921 aagtcatcgt cgacacatcc aaggcggaac tctgttttgc cgacgaccgt cagtgccacc
    13981 ccgtcctcat cggcactgcc acacccaagg ggacgttcgg actgacgctg aacagtaccg
    14041 acaagcccgg ttacggcggc gaagtcatcg gtttcaagca ggagggcgat tttcttttcg
    14101 ccctgcaccg cgtttggaat cagataccgt ccgaaaggcg aaacgaacgc atcgcctccc
    14161 cgtccgtgtc cgacaggatt atgaccaacg gctgcatcaa cgtcagcgat gcggtgtacg
    14221 aaaaactgcg tcattatttt gtgttggaag tgatttgaaa cagacggata ccgcacgtgc
    14281 cggtatccgt tttcacattg ccccgatgcc tgaaacagac cgtccgtcac gtcatgccgc
    14341 ctgaaaccgg cgcagatgcc gccaagcctt cagacggcat tgtccgcccg ctccgaccga
    14401 acaacaacca tctttgggag aaccttatga ccgaacaaaa ccgcatcctc tgccgcgaac
    14461 tgagcctgct ggcattcaac cgccgcgtgt tggcacaggc ggaagacaaa aacgtccccc
    14521 ttttggaacg cctgcgcttc ctgtgcatcg tgtcgtccaa tcttgacgag ttttttgaag
    14581 tccgcatggc gtggctcaag cgtgaaaaca agctgcaccc ccggcgcagg ccggacaacg
    14641 gcaaaacgcc gtctgaaacc atcgccgacg ttaccgaagc ggcgcgctcc ctgatacggc
    14701 accagtacga cctgttcaac aacgtccttc agcccgagtt ggcgcgggaa agcatccatt
    14761 tttaccgccg ccgcaattgg acgggcacgc agaaaaaatg gatcgaagac tatttcgacc
    14821 gcgaactgct gccgatcctg acccccatcg gactcgaccc ttcccaccct ttcccgcgcc
    14881 cgctgaacaa atcgctcaac ttcgccgtag aactcgacgg cacggacgcg ttcggcaggc
    14941 cttcgggtat ggcgattgtc caagcaccgc gcatcctgcc gcgcgttgtt cccctgccgt
    15001 ccgaactgtg tggcggcgga cacggcttcg tcttcctgtc gtccatcctc cacgcccacg
    15061 tcggcaaact cttcccgggc atgaacgtca aaggctgcca ccagttccgc ctcacgcgcg
    15121 acagcgactt gaccgttgac gaagaagacg tacaaaacct ccgcgccgcc attcaaaacg
    15181 aactgcacga ccgcgaatac ggcgacggcg tgcggctcga agtcgccgac acctgtcccg
    15241 cctacatccg cgactttctg ctcgcgcaat tcaggctgac cgacgccgaa ctctatcagg
    15301 tcaaaggccc ggtcaacctc gtccgcctca acgccgtccc cgacctagtc aaccgccccg
    15361 atttgaaatt tccgccgcac acgccgggcc gtctgaaagc cttgggcaaa aacagcccga
    15421 tcttcgattt ggtgcgccaa tcgcccatcc tgctgcacca cccctaccaa tctttcgatc
    15481 ccgtggtcga tatgatacgc gaagccgccg ccgatcccgc cgttcttgcc gtcaaaatga
    15541 cgatttaccg caccggcacg cgctccgaac tcgtccccgc cctgatgaag gcggcactcg
    15601 ccggcaaaca agtaaccgtc gtcgtcgaac tgatggcgcg ttttgacgaa gccaacaacg
    15661 tcaactgggc gaagcagctc gaagaggcgg gcgcgcacgt cgtgtacggc gtgttcggct
    15721 acaaagtcca cgccaaaatg gcactggtca tccgccgcga agacggcgtg ctcaagcgtt
    15781 acgcccacct cggcacgggc aactaccacc aaggcacatc gcgcatctac accgacttcg
    15841 gcctcattac cgccgacgaa caaatcaccg ccgacgtgaa cacattattt atggaaatca
    15901 caggcttggg caaacccggg cggctgaaca aactctacca aagtccgttt accctgcaca
    15961 aaatggttat cggccgcatc gctcgcgaaa ccgaacacgc aaaagccggc aaaccggcgc
    16021 ggattaccgc caagatgaat tcgctcatcg aaccgactgt catagaagcc ctgtatcggg
    16081 caagcgcggc aggcgtgcaa atcgatttga ttgtgcgcgg tatgtgtacc ttgcgcccgg
    16141 gtgtaaaagg cttgtccgaa aacatccgcg tccgctccat cgtcggcagg cagctcgaac
    16201 acgcgcgcgt atattgtttc cacaacaacg gcgcggacga cacctttatt tccagcgcgg
    16261 attggatggg gcgcaatttc ttccgccgca tcgaaaccgc caccccgatt accgcgcccg
    16321 aactcaaaaa acgcgtcatc cgcgaaggtt tggaaatggc actggcagac aatacccacg
    16381 cgtggctgat gcagcccgac ggcggctata tccgcgccgc gcccgccgaa ggcgaaagcg
    16441 aagccgacct gcaaaacgat ttatgggatt tgctccgagg ctgacccgca caagcacttc
    16501 ccccatacgc tcccaacccc aatccgccgc attccagacg gcattgcctt tccgacagcc
    16561 gaacaaaacc ccgaatccgt ctgcacggtt cggggcatat ctccaatacg ggcatcgtgt
    16621 tccggaaaac cgtcaaatcc gcatcggcat cacaatatat ttgaaattcg gattgttcgg
    16681 cacggtaaac agcgtcgagc ggttggcgtc gccgaaggca agctgcatat cgtcggaatg
    16741 gatgttgcgc aacacgtcca tcaaatagcc gatattgaaa ccgacttcga gttcgccgcc
    16801 ctgataggcg atttcgattt cttcgcgcgc ttcttcctgc tcgttgttgc tgcacacgac
    16861 gctcaacagg ccgggttgca ggaacaggcg cgcgccacgg aatttttcgt tggcaagaat
    16921 ggacacacgt tccagcgcgc ccaaaagttc ggcacgggac aacacgaaaa tcttgtcgtt
    16981 gtccaaagga atcacgcggt tgaaatcggg gaatttgccg tcgatgacct tgctgacgat
    17041 ggtcgtgccg ttgcattgga aacgcacctg cttgtccagc agctcgattt gaatcggatc
    17101 gtcggggttg ttcaacagtt tgaacagttc cagcaccgtt ttgcgcggca aaatcacttc
    17161 ggcgcgcggc aaatccgcat caatcgcgca ggctgcatag gcaaggcggt gtccgtcggt
    17221 cgccacaagg cgcaactggc tgccctcaac ctgcatcagc agaccgttga gataatagcg
    17281 gatgtcctgc accgccatgc tgtactgcac ttgcgacagc atggttttga aacgctcctg
    17341 ccccagcgag aaagtcgcgc tgatgtcctc gccgacattc atcatcggaa aatcggcggc
    17401 aggcagggtt tgcagggcaa aacgcgattt gcccgccttc agcgtcagac ggttgtcgtc
    17461 ccaatccagc gacaccagcg caccggcagg cagcgcgcgc aaaatgtcct gaaatttctt
    17521 ggcattggta gtgatgcgga agtcgcccgc acctccctcg ggacccgcgg tgtcgatttg
    17581 gatttccaaa tcggttgcca agagtttggt ctgaccgccc ctgccctcaa tcagcacatt
    17641 ggacaggatg ggcagggtgt gtcggcgttc gacgatgccg gtaacggctt gcagcggctt
    17701 gagcaggctg tcgcgctcgg cttgtaaaat caacattttc gctccttaaa tcggttggat
    17761 ttcctaccct tcggacggca tggtcaatgt catgccgtct gaaaggcgtg tccggtcagt
    17821 tttgaatcag aatcagcagt ttctcgtaat cctgcgccaa ctcggggtct tcctcgcgca
    17881 gtttcgccac cgccctgatg ccgtgcatga cggtcgtatg gtcgcgtccg ccgaacgaat
    17941 cgccgataga cggcaggctc aaagtggtca attctttggt caggctcatg gcaacctgac
    18001 gcggacgggc aatgttgcgc gtgcgttttt tgccgagtac gtcgctgatt ttgatgcggt
    18061 aatatttcgc caccgcatcg atgatgatgt cggcggtgat gactttgtgc ttctcggcaa
    18121 taatgtcctg caaagcggta cgcgccaaat cgatgtcgat gacgggacgg ttcataaagc
    18181 ggctgctcgc tccgacacgg ttgaacgcgc cttccagttc gcgcacgttg gaacggatca
    18241 gattggcaat gaacagcgcg gcttcgtctt cgatactgat gcccgccgct tccgcctttt
    18301 tctgcaaaat ggcgatacgc atttccaatt cgggcggctc gagttccaaa gtcagccccc
    18361 acgaaaagcg ggatttgagg cggtcgtcca taccttcgat tttggcgggt aaaacatcgc
    18421 aagtgaggat gagctgtttt ttctcattgt gaaaatggtt gtacagatag aaaaattctt
    18481 ccatcgtacg gtcttttcct ttgatgaact gaatatcgtc gataatcagc aggtcgtatt
    18541 gcttgtattg ctgcttgaac acgtcgtagg tattgttgcg aaccgccttc ataaagctgc
    18601 ggatgtagtc gtccgaatgc atatagcgca ctttggcatc gggacggttt ttcaacagct
    18661 cgttgccgac cgcctgcaca aggtgggttt tgcccaaacc cgtgctgccg tacaggaaga
    18721 acgggttgta actctgcccc gggttttccg caatcgcctg cgccgcagcc gccgcaaggc
    18781 ggttgccctt accttctacc aacgtatcaa acgtgtaatc cggagacagg ttggtttgtt
    18841 cgtaacgcgc ttcttccgca tcacgctgcg catccgtccg cgctttggca actgccgccg
    18901 attccggccg ggaagcaggc ccggcagcct gacgcggctc gtgcggcagg tttttcatac
    18961 gttccgccaa aatatccgcc gccgttttcg acgcagcggg tttgacaggc ttttcagacg
    19021 gcagctcgtc caacagaacc tcctgcacgg gcatctcctc cgaccccgcg tgcaaggacg
    19081 gctcggcagg ttcgacagca ccttcaaccg ccgccatctc ataacgcacg ccttctcccg
    19141 gtttgaatac gaaggcggga cggccggcag ccaactcttc cctcaccgct tctatttttc
    19201 cggcaaactg gctcttgagc atattgcagg caaactggtt cttgccgtac accacccata
    19261 cgccaccctc ctcaccaacc gtaaggggcg caatccattg cgcaaactgc ccgtgaggca
    19321 acatatcgtg aagacggcgg aggcacagcg gccaaaactc tgctaatgtc atggataggc
    19381 tcgaatcggt aaaaatgaaa tcgaaaacaa agaaaatata atattttcaa aaagaaaaca
    19441 aatctgttga atacgcgtcg gttcaaaacg cagcgaaccg attataccga ctcgccgata
    19501 ttttatccac aacccgtgca aaaatttatc cacagaaagg cggcagaaat ccgcaggcaa
    19561 tcgggcaatc ctcctgcaaa gtttctatat tgattgacaa aagcggcaaa ttggagtgta
    19621 attcacggtt taattatcta cccattctat tttaggaacc atcatgaaac gcacttatca
    19681 accttccgtt accaaacgca aacgcaccca cggcttcctc gtgcgctcca aaacacgcgg
    19741 cggacgcgca gtattggccg cacgccgtgc caaaggccgc aaacgcctgg cagtgtaatt
    19801 ttggactacc gcttcggaag gcagtaccgc ttgttaaaaa cggatgattt ttcatccgtt
    19861 tttgcattca gaaaccgccg cagccgcgac ctgctgcaag tttcgcgctc aaacggcaac
    19921 gggctggacc atccccgcat cggtctggtg gtcggcaaaa aaaccgccaa acgcgccaac
    19981 gagcggaact atatgaaacg cgtcatccgc gactggttca gattgaacaa aaaccggctg
    20041 ccgcctcagg atttcgtcgt gcgcgtccgc cggaaattcg acagggctac cgcaaaacag
    20101 gcaagggcgg aactggcaca actcatgttc ggcaaccccg caaccggatg cgggaaacag
    20161 gtatgatcag aacggtaccc tgcaggcaag gttcagacgg caacgggttt cccatacaag
    20221 gaacatcccg atgaacttcc tattgtccaa actgctgctg gggctgataa ggttttacca
    20281 atactgcatc agcccgctga ttccgccgcg ctgccgttat acgccgacct gttcgcaata
    20341 cgcggtcgaa gcggtcaaaa aatacggcgc attcaaaggc ctccggctcg ccatcaagcg
    20401 cattgcacgc tgccaccctt tcggcggaca cggacacgac cccgttccct gacccgacgc
    20461 aatattcaaa ttgcacgctt tccttttatt tcccatcggt ttctatataa tgccgtctga
    20521 accttcgggc tggcggcacg gccgtcgggt atgaagcccg cccttattcc ccgtctatcg
    20581 gaacacgcaa cctgcggcat ttccgaccat tcaggaaact ctatatggat tttaaaagac
    20641 tcacggcgtt tttcgccatc gcgctggtga ttatgatcgg ctgggaaaaa atgttcccca
    20701 ccccgaaacc cgtccccgcg ccccaacagg cggcacaaaa acaggcagca accgcttccg
    20761 ccgaagccgc gctcgcgccc gcaacgccga ttaccgtaac gaccgacacg gttcaagccg
    20821 ttattgatga aaaaagtggc gacctgcgcc ggctgaccct gctcaaatac aaagcaaccg
    20881 gcgacgaaaa caaaccgttc gtcctgtttg gcgacggcaa agaatacacc tacgtcgccc
    20941 aatccgaact tttggacgcg cagggcaaca acattctgaa aggcatcggc tttagcgcac
    21001 cgaaaaaaca gtacaccctc aacggcgaca cagtcgaagt ccgcctgagc gcgcccgaaa
    21061 ccaacggact gaaaatcgac aaagtctata cctttaccaa agacagctat ctggtcaacg
    21121 tccgcttcga catcgccaac ggcagcggtc aaaccgccaa cctgagcgcg gactaccgca
    21181 tcgtccgcga ccacagcgaa cccgagggtc aaggctactt tacccactct tacgtcggcc
    21241 ctgttgttta tacccctgaa ggcaacttcc aaaaagtcag cttctccgac ttggacgacg
    21301 atgcgaaatc cggcaaatcc gaggccgaat acatccgcaa aaccccgacc ggttggctcg
    21361 gcatgattga acaccacttc atgtccacct ggatcctcca acctaaaggc ggccaaagcg
    21421 tttgcgccgc aggcgactgc cacatcgaca tcaaacgccg cagcgacaag ctgtacagcg
    21481 caagcgtcag cgtgccttta gccgctatcc aagccggcgc caaagccgaa actgccgtca
    21541 acctgtatgc cggtccgcaa accacatccg ttatcgcaaa catcgccgac aacctgcaac
    21601 tggcaaaaga ctacggtaaa gtacactggt tcgcatcgcc gctcttctgg ctcctgaacc
    21661 aactgcacaa cattatcggc aactggggct gggcaatcgt cgttttgacc atcatcgtca
    21721 aagccgtact gtatccattg accaacgcct cctaccgttc gatggcgaaa atgcgtgccg
    21781 ccgcacccaa actgcagacc atcaaagaaa aatacggcga cgaccgtatg gcgcaacagc
    21841 aagcgatgat gcagctttac aaagacgaga aaatcaaccc gctgggcggc tgtctgccta
    21901 tgctgttgca aatccccgtc ttcatcggct tgtactgggc attgttcgcc tccgtagaat
    21961 tgcgccaggc accttggctg ggctggatta ccgacctcag ccgcgccgac ccctactaca
    22021 tcctgcccat cattatggcg gcaacgatgt tcgcccaaac ctatctgaac ccgccgccga
    22081 ccgacccgat gcaggcgaaa atgatgaaaa tcatgccgtt ggttttctcc gtcatgttct
    22141 tcttcttccc tgccggtttg gttctctact gggtggtcaa caacctcctg accatcgccc
    22201 agcagtggca catcaaccgc agcatcgaaa aacaacgcgc ccaaggcgaa gtcgtttcct
    22261 aaatgccgca gcatgaaaaa tgccgtctga aacctgttca gacggcattt ttattgttca
    22321 ccccctatcg gggcggaaat cttcaacccg catacatcac aaaaatcgtc gggcgttttt
    22381 tcagattggg catttccttc atcctgcgcc aatccgcaac cgttttactg accaccaact
    22441 gagtcggcaa cgtcaaatcc gtagccgtac acaagcgcgt ttcaggatgc aggttttcca
    22501 ccgcatcggc aagcagcgca tcattgcggt aaggcgtttc aataaaaatc tgcgtctcgc
    22561 cgcactgccg cgaacgttgc tccaaagcat tcaaactctg aatgcgctca tttttttcag
    22621 acggcagata accattaaac gcaaagttct gcccattcgc acccgaagcc atcagcgcca
    22681 gcagcaggct ggacgggccg accaaaggcc gtacttcaaa accgtgttta tgcgccaatg
    22741 ccaccaaatt cgcgcccgga tcggcaacag ccgggcaacc cgcctcgctg acaatcccca
    22801 tactgcgccc ttcttgcaaa ggtttcagca attccggcaa agtcttcaaa tccgtgtgtt
    22861 cattcaacgt ttgcagattc agttcgcgta tcggcgtcgt tatccccaaa tgcttcaaat
    22921 gcgcacgcgc cgttttttcc gcttccacga caaaatccgt cagcccgaca accgcccgtt
    22981 gttcatgctg caacaggcac ggcgtgtcag gcgtacccaa aggcgtagga atcaaataca
    23041 aaacaggaga catcattccc tcactcatcg gttaaaaatg ccgtccgaac ccttcagacg
    23101 gcataaacgg gcagttacaa aacatccacg ccctcgtttt tcaaaaaatc gaccagacgg
    23161 aaaaccggca gaccaatcaa ggcattcgga tcggtacttt caatccgttc gatcaacagc
    23221 gcgcccaagc cctcgctttt cgccgcacac gaacaataaa ccgcatccgg ctcgcgctcc
    23281 aaatagcgga ggatgtgcag ctcgtccaac tgcctcatca cgaccaccgt cttatcgata
    23341 tgccgatgca tcctgcccgt aaccgtattt agcagaacga ccgcactgta aaactcaatc
    23401 tccctgccgc tcaaatgcat caacatcttt tgcgcgttgg caaggttcat cggcttgccc
    23461 cactgcctgc cgtcgcacca cgccacctgg tccgcaccga caatcaacgc cccggggaaa
    23521 cgcccggcca acgaccgcgc cttaccctcg gcaaggcgca atgccgtctg aggggcggat
    23581 tcccccaaca tcggcgtttc gtcaaaatcg ggggatgccg cctgaaaggc aatgccgagc
    23641 ctttccatct gttcgcggcg gaaaaccgaa ctcgtaccca aaaccaaagg cagttccaaa
    23701 cccatcccat cctccttacc gttgaaaaca cgcccgaagg ggcggtaaaa tccagccatg
    23761 cgccgaaaca cggacacccg ccttcggcgt accgcaacat ttttcttaaa aatattgacg
    23821 ttagaacatc taaattatat cataccccgt ttatgtcaga ccctaatttg attgacccgg
    23881 aaatttttgc cgccgaaagg cagaacctgc aaggcagttt tctgctggaa gaattggacg
    23941 agcgagtcag tttgcacgat tatcccgccg acaggcggac caaaatatcg tttacactga
    24001 ccggcggtcg cgaccggctg caacgcctgt tcctcgacct gaacgtcaaa gccgatatgc
    24061 ccctgatttg ccagagatgt atcaaaccca tgccgttcat gctcgatgaa agcagccgta
    24121 tcgtcctgtt ttccgacgaa gagtccttgg acgaatccat gcttgccgac gaagaactcg
    24181 aaggcatact gattgaaaaa gaactcgacg tgcgcgcatt ggtagaagac caaatcctga
    24241 tgtccctgcc cttttcgccg cgacacggac actgcggcaa tacccttccg gaatccgcca
    24301 accaagacaa acccaacccc tttgctgttt tggcgggttt gaaaagcagt taattaggac
    24361 acagtttatt tatctaggag cttgaaatgg ccgttcaaca aaacaaaaaa tccccttcca
    24421 aacgcggtat gcaccgttcg cacgacgcac tgaccgcgcc cgcactgttt gtcgacagca
    24481 caaccggcga agtacaccgc ccgcaccaca tctcccccaa cggtatgtac cgcggtcgca
    24541 aagtggtcaa agccaaaggc gaataatccc tattcgactg actgaaaaag ccagaacatt
    24601 gccatgcaat tactggcttt ttttgcattg gacgcaccat ccgtccaaac tttcgccata
    24661 cgtcaacaca caggggcaaa gcgttccgta taataccccg tgaaaatatt ccaaaagccc
    24721 caaccaccaa ggaaattccg atgaaacaga aaatctggta cacctacgat gacatccacc
    24781 gcgtcatcaa agcattggca gaaaaaatcc ggaacgccgg cgtcaaatac gatgccatga
    24841 tcgctatcgg cggcggcggc tttattccgg cacgcatgct gcgctgtttt ctggaaattc
    24901 cgatttatgc cgtaaccacc gcctattacg acagcgacag cgaaggacaa gttaccgaag
    24961 aagtcaaaaa agtccaatgg ctagaccccg tccccgaagt cctgcggggc aaaaacgtgc
    25021 tcgtcgtcga tgaagtggac gacagccgcg taaccatgga gttctgcctg aaagaactgc
    25081 tcaaggaaga cttcgacact gtcggcgtcg ccgtcctgca cgaaaaaatc aaagccaaag
    25141 caggcaaaat ccccgaaggc attccctatt tcagcggcat taccgtagaa gactggtgga
    25201 tcaactatcc gtgggacgca ctcgacatcg acgaacacaa ccgccttgcc gaagccggcc
    25261 gaggctaacc ctttcagacg gcatattttc cgaaccgatg ccgtctgaag cccgcacgac
    25321 ccctgccgca gaccgaaaat ctaccggaga aacccctatg attacattgg ccgtagatgc
    25381 catgggcggc gaccaaggac ttgccgttac cgtacccggc gcaaccgcat tcctccaagc
    25441 acaccccgat gtccgcctga ttatgaccgg cgacgaaacg cagctccgcc aagccctgaa
    25501 cgcggcaggc gcgccgatgg aacgcatcga catctgccat accacccagg ttgtcggcat
    25561 ggatgaatcc cctcagtccg ccctaaagaa caaaaaatac tcctccatgc gcgttgccat
    25621 caaccaagtt aaagaaggca aagcccaagc cgccgtatcc gcaggcaaca cgggcgcgct
    25681 catggcaacc gcacgtttcg tcctcaagac catacccggc atcgaacgcc ccgccatcgc
    25741 caaattcctt ccttccgaca ccgaccacgt taccctcgcc ctcgacctcg gcgcaaacgt
    25801 cgactgcaca cccgaacagc ttgcccaatt tgccgtcatc ggcagcgaac tcgtccacgc
    25861 gctccatcct caaaaaggac agccgcgcgt cgggctggtc aacgtcggca cagaagacat
    25921 caaaggtacg gataccgtca aacaaaccta caaactgctg caaaacagca aactcaactt
    25981 tatcggcaac atcgaaagca acggcatcct gtacggcgaa gcagatgtcg tcgtcgccga
    26041 cggctttgtc ggcaacgtca tgctcaaaac catcgaaggc gcagtcaaat tcatgagcgg
    26101 agccatccgc cgcgaattcc agagcaacct gttcaacaaa cttgccgccg ttgccgccct
    26161 acccgccctc aaaggcttga aaaacaaact cgatccgcgc aaattcaacg gggccatcct
    26221 gctcgggctg cgcggcatcg tcatcaaaag ccacggcggc acagacaaaa ccggtttccg
    26281 ctacgcactc gaagaagcct accacgaagc caagtccgcc ggcctttcca aaatcgaaca
    26341 aggcgttgcc gaacaactcg ccgcactgga agccgcccaa aacgaaaccg ccgccagtct
    26401 gtaacacgca cgatgccgtc tgaacgcccc cgcccctttc agacggcatc cggcacccgt
    26461 ccaaacctgc gggcgcggac ggcgatgcgc ctgtccggca cttcccaaat acttccctgt
    26521 aaaataagga gtatttgaaa aatgaagaca ttagaaaaac ggatgaaagc tctagacaaa
    26581 cggatgatga agttcggaaa atcccttgaa ggcaggcttg atgcccgtct gattgaatcc
    26641 gcattggatt atattcatta ttcggaacgt tttttggctt ttgacatcct gtgtacttat
    26701 atcgaagatt tcgatatccg gctgacggaa caagaatccc gggaaatttc ttttatcaac
    26761 aaggaatttg agatagaaag cacgtccgat taaccaataa agccaatgat ttgataagaa
    26821 tgaaaacatc gacggtcgtt tttggcggat tttttatggc agacaacggg gagcgaatcc
    26881 aaatccccgt tttggaaaat cctgacatta gggaaatcaa tcgctttttt tccgtatcaa
    26941 attttgagaa aaaagccggc gtccttgttt tcagaatcat ccccgagccg gaatttggca
    27001 ataccgaatt aactgtctat tttgaaaaag gatattatag tggattaaca aaaaccggta
    27061 cggcgttgcc ccgccttggc tcaaagggaa cgattcccta aggtgcccaa gcaccaagtg
    27121 aaccggttcc gtactatccg tactgtccgc ggcttcgccg ccttgtcctg atttttgtta
    27181 atccactata tttgccaata attcaaacaa ttttagaagg cggggatatt aaagtaaaaa
    27241 accttaaaac agaaaattac agcgggaagg caagggaaat cttgggagat tgctacccta
    27301 ttgaacatat ctctaaaaat atttctacaa ttcaaaatat catttccgaa tttatcagac
    27361 aaaaacaaac accggcacca aataatttga tggaaaccca acaatcaaga tgccgtctga
    27421 agcagcttgg gctttcagac ggcatttcct tcgcttaaaa cagcgtatcg gcaaccccgc
    27481 cccgcccgtc cacggcaatc cgcatctgaa aaccatctgt atgccaaacc acacccccat
    27541 ccctgtttcc gtcatttgca ccctgcccgt attgggcaat catcggtttt tcgcttacaa
    27601 tagccgaatc tgaaccaact ctctaaaaag gccgttccca tgcagtatgc aaaaatttcc
    27661 ggcacgggca gctatcttcc tgccaaccgc gtcagcaatg acgaccttgc ccaaaaggta
    27721 gatacctctg acgagtggat taccgcgcgt acgggcatca aattccgcca tattgcagcc
    27781 gaaaacgaaa aaaccagcga tcttgcagcc gaagcggcgc ggcgcgcttt ggcagatgcc
    27841 aaattaaata taaacgacat tgatttgatt atcgtggcga cggcaacgcc ggatatgcag
    27901 tttccttcta ccgccaccat cgtgcagcaa aaattgggca tcaccaacgg ctgccccgcg
    27961 tttgacgtgc aggcggtgtg cgcaggcttt atgtacgcgc tgactacggc aaatgcctac
    28021 attaaaagcg gtatggcgaa aaacgcgctg gtcatcggcg cggaaacctt cagccgcatc
    28081 gtcgattgga acgaccgcac aacctgcgtg ctgttcggcg acggcgcggg cgcggtggtt
    28141 ttaagcgcgt ccgacaaacc gggcatcatc cacagcaaac tcaaagccga cggcaattat
    28201 ctgaaactct taaacgtccc cgggcaaatc gcctgcggca aagtttccgg ttcgccgtac
    28261 atttcgatgg acggtcccgg cgtgttcaag tttgccgtca aaatgctgtc caaaatcgcc
    28321 gatgacgtta tcgaagaagc aggttacacc gccgaacaaa tcgactggat tgttccccat
    28381 caggcaaacc gccgcattat cgaatcgacc gcgaaacatt tgggtttgag tatggacaaa
    28441 gtcgtcctga ccgtccaaga ccacggcaac acgtccgccg catccattcc gctggctttg
    28501 gacgcgggca tccgcagcgg acaaatcaaa cgcggtcaaa acctgctgct cgaaggcata
    28561 ggcggcggtt tcgcgtgggg cgcggtgctg ttgcaatatt gaaaccggtg ccgtctgaaa
    28621 caggctttca gacggcattt cccatatcat gaagcggcag gctttcttca aaccgatggc
    28681 gtgtgcggca tttctgtccg ccgtttcgct gcgcctcccc gtattgggcg cgtgttacgc
    28741 aatattgtcc ctctatgcgt ttgcacttta cggcatcgac aaacggcgtg ccgtgcgggg
    28801 aaaacgccgc attcccgaac accgcctgct cctgcctgcc ttgttcggcg gttggacggg
    28861 cgcatacttg ggtagtagga tgttcaggca taaaacggcg aaaaagcgtt ttgttgtgct
    28921 gttccgtctg actgtttcgg gcaatgtcct ggcgacctgc atcctgattg attatttcgt
    28981 tccgcccgaa ctttttgtaa aactcgggca acatctctga caatccaacc caacaaagga
    29041 atactatgtc tttcgcattc ttcttccccg gacaaggttc ccaaagcctc ggcatgatga
    29101 acggctttgc cgaacacgcc atcgtcaaaa acacatttga cgaagcctcc gccatattgg
    29161 ggcaggactt gtgggcgatg attaacggca gcgatgccga aatcatcggt caaacagtca
    29221 acacccagcc cattatgctc gccgccggcg ttgccgttta ccgcgcctat ttggaagtgg
    29281 gcggcaaaac gcctgccgcc gttgccggac acagcctcgg cgaatacacc gcgcttgtcg
    29341 ccgccgaagc attggatttt gccgatgccg tcaaactcgt ccgcctgcgc gccgaactga
    29401 tgcagtccgc cgtaccgcaa ggcgtaggcg caatggcggc cattttgggt ttggaagatg
    29461 aacaggtcag gcagatttgt gccgaatccg cacagggcga agtggtcgaa gccgtcaact
    29521 tcaactcgcc cggacaagtc gtgattgcag gcaacgccgc cgccgtcgga cgcaccatgg
    29581 ctgccgccaa agaagccgga gccaaacgcg ccctgccgct gccggtatcc gttccttccc
    29641 attgcagcct gatgaaaccc gccgccgaca aacttgccga agcactcaaa accgttgaaa
    29701 tcaaacagcc gcaaatccgc gtcatccaca acgccgacgt tgccgcctac gatgatgccg
    29761 gcaaaatcaa agacgcgctc gtccgccagc tttacagccc cgtgcgctgg acggaaaccg
    29821 tcaacgccct tgtttcagac ggcattgccg aatccgccga atgcggtccg ggcaaagtgt
    29881 tggcgggctt ggcaaaacgc atcaacaagg ccgccgcttg cagcgcactg accgatgccg
    29941 gacagattac cgcctttatc gaagcgcact gacttcgttc cgcaaaaagc agcctgccct
    30001 cttcaggctg cttttcacgt ccgaacggca aacagccgca tatttgcgct ataattcatc
    30061 cctaccaaac caccgacagc ggctgccgtt gcagttcccg ccctaccgat atgatagaaa
    30121 aactgacttt cggactgttt aaaaaagaag acgcgcgcag ctttatgcgc ctgatggcgt
    30181 acgtccgccc ctacaaaatc cgcatcgttg ccgccctgat tgccattttc ggcgttgccg
    30241 ccaccgaaag ctacctcgcc gccttcatcg cccccctgat caaccacggc ttttccgcac
    30301 ccgccgcgcc gcccgagctg tccgccgccg gcatcctttc caccctgcaa aactggcgcg
    30361 aacagtttac ctatatggtt tgggggacgg aaaacaaaat ctggaccgtc ccgctcttcc
    30421 tcatcatcct cgtcgtcatc cgtggcatct gccgctttac cagcacctat ctgatgactt
    30481 gggtctccgt gatgaccatc agcaaaatcc gcaaagatat gtttgccaaa atgctgaccc
    30541 tttcctcccg ctaccatcag gaaacgccgt ccggcaccgt actgatgaat atgctcaacc
    30601 tgaccgaaca gtcggtcagc aacgccagcg acatcttcac cgtcctcacg cgcgacacga
    30661 tgatcgttac cggcctgacc atcgtcctgc tttacctcaa ctggcagctc agcctcatcg
    30721 tcgtcctgat gttccccctg ctctccctgc tctcgcgcta ctaccgcgac cgtctgaaac
    30781 acgtcatttc cgactcgcaa aaaagcatag gcacgatgaa caacgtgatt gccgaaaccc
    30841 atcagggaca ccgcgtcgtc aagctgttca acgggcaggc gcaggcggca aaccggttcg
    30901 acgcggtcaa ccgcaccatc gtccgcctca gcaaaaaaat cacgcaggca acggcggcac
    30961 attccccgtt cagcgaactg attgcctcga tcgccctcgc cgtcgtcatc ttcatcgccc
    31021 tgtggcaaag ccaaaacggc tacaccacca tcggcgaatt tatggcattc atcgtcgcga
    31081 tgctgcaaat gtacgccccc atcaaaagcc ttgccaacat cagcatccct atgcagacga
    31141 tgttcctcgc cgccgacggc gtatgtgcat ttctcgacac cccgcccgaa caggacaaag
    31201 gcacgctcgc gccgcagcgt gtcgaagggc gcatcagctt ccgcaacgtc gatgtcgaat
    31261 accgttcaga cggcatcaaa gccctcgaca acttcaacct cgacatcaga caaggcgaac
    31321 gcgtcgccct ggtcggacgt tccggcagtg gcaaatccac cgtcgtcaac cttctgcccc
    31381 gctttgtcga accgtctgcc ggcaacatct gcatagacgg tatcgacatc gccgacatca
    31441 aactcgactg cctgcgcgcc caattcgccc tcgtctccca agacgtattc ctgtttgacg
    31501 acaccctgtt tgaaaacgtc cgatacagcc gtcccgacgc tggcgaatcc gaagtcctgt
    31561 ccgccctcca agccgccaac ctgcaaagcc tgatcgacgc atccccgctc ggactgcacc
    31621 agcccatcgg atcgaacggc agcaacttat ccggcggaca gcggcaacgc gtcgccattg
    31681 cccgcgccat tttgaaagac gcgccgatat tattattgga cgaagccacc agcgcattgg
    31741 acaacgaatc cgaacgcctc gtccaacaag cgctcgaacg cctgatggaa aaccgcaccg
    31801 gcatcatcgt cgcccaccgc ctgaccaccg tcgaatccgc cgaccgcatc atcgtcatgg
    31861 atggcggcaa aatcatcgaa caaggcacac acgatcaact catgttccaa aacggctact
    31921 acaccatgct gaggaacatc tccggaaaag ataccgccgc cgttcagacg gcataaacaa
    31981 aatgccgtcc gaaatggtat aatcgccccg accccttcag acggcactat atccgccgac
    32041 ccatccgatt atcttcaacc accgtaaaac ccattatgac ccaagacaaa atcctcatcc
    32101 tcgacttcgg ttcgcaagtt acccggctga tcgcccgccg cgtgcgcgaa gcacacgttt
    32161 actgcgagct gcattccttc gatatgcctt tggacgaaat caaagccttc aaccccaaag
    32221 gcatcatcct ttccggcggc cctaattctg tttacgaatc cgactatcaa gccgataccg
    32281 gtatttttga tttgggcatt ccggttttgg gcatctgcta cggcatgcag tttatggcgc
    32341 accacttggg tggcgaagtg cagcccggca accagcgcga attcggttac gcgcaagtca
    32401 aaaccatcga cagcggactg acacgcggca ttcaagacga cgcgcccaac acactcgacg
    32461 tatggatgag ccacggcgac aaagtgtcca aactgcccga cggtttcgcc gtcatcggcg
    32521 acactccgtc ctgcccgatt gccatgatgg aaaacaccga aaaacaattc tatggcatcc
    32581 agttccaccc cgaagttacc cacaccaaac aaggccgcgc cctgttgaac cgctttgtct
    32641 tggatatttg cggcgcgcaa ccgggctgga cgatgcccaa ctacatcgaa gaagccgttg
    32701 ccaaaatccg cgaacaagtc ggcagcgacg aagtgatttt aggtctgtcc ggcggcgtgg
    32761 actcttccgt agccgccgcg ctgattcacc gcgccatcgg cgaccaactg acctgcgtgt
    32821 tcgtcgatca cggtttgttg cgcctgaacg aaggcaaaat ggtgatggat atgttcgccc
    32881 gcaacttggg tgtgaaagtg atacacgtcg atgccgaagg gcagtttatg gcgaaactcg
    32941 ccggcgtgac cgaccctgag aaaaaacgca aaatcatcgg cgcggaattt atcgaagtat
    33001 ttgatgccga agagaaaaaa ctcaccaacg ccaaatggct ggcgcaaggc acgatttacc
    33061 ccgacgtaat cgaatccgcc ggtgcgaaaa ccaaaaaagc ccacgccatc aaatcccacc
    33121 acaacgtcgg cggcctgcct gaaaatatga agctcaaact gcttgagccc ttgcgcgact
    33181 tgttcaaaga cgaagtgcgc gagttgggcg tggctttggg cctgccgcgc gaaatggtgt
    33241 accgccaccc cttcccgggc cccggtttgg gtgtgcgcat cttgggcgaa gtgaaaaaag
    33301 aatacgccga cttgctgcgt caggcggacg atattttcat ccaagaatta cgcaatacta
    33361 ccgacgaaaa cggcacgtct tggtatgacc tgaccagcca ggcatttgcc gtattcctgc
    33421 ccgtcaaatc cgtcggcgtg atgggcgacg gccgcactta cgactacgtc gtcgcactgc
    33481 gcgcagtcat caccagcgac tttatgactg cacactgggc agagctgcca tactcactgc
    33541 tcggccgcgt gtccaaccgc atcatcaacg aagtcaaagg catcaaccgc gtggtgtacg
    33601 atgtcagcgg caaaccgccc gccaccatcg agtgggaata aacagcaaac agggctgcac
    33661 cgtccggcgc agtccttcga ttatcggaaa aaaggaaaaa atatgagcac acaagattta
    33721 agcggcaaaa tcgctttggt aaccggcgca tcgcgcggca tcggcgcggc aattgccgac
    33781 acactggcgg cagccggtgc caaaatcatc ggtacggcaa ccggcgagag cggtgcggcg
    33841 gcgattagca aacgtctggc acaatggggc ggcgaaggcc gcgtattaaa ttccgccgaa
    33901 cccgaaaccg tcgaaaacct gattgccgac atcgaaaaaa cgttcggcaa actcgacatt
    33961 ctggtcaaca acgccggcat cacccgcgac aacctgttga tgcgtatgaa agaagaagag
    34021 tgggacgaca tcatgcaggt caacctcaaa tccgtgttcc gcgcttctaa agccgtcttg
    34081 cgcggcatga tgaagcagcg cgccggccgc atcatcaaca tcacctccgt cgtcggcgtg
    34141 atgggcaatg ccgggcaaac caattatgcc gccgcaaaag cgggcttgat cggttttgcc
    34201 aaatctatgg cgcgcgaagt cggcagccgg ggcattaccg tcaactgcgt cgccccgggc
    34261 tttatcgata ccgatatgac ccgcgccctg cccgaagaaa cccgccaaac atttaccgcc
    34321 caaaccgcct tgggcagatt cggcgacgcg caagacatcg ccgatgcggt tctgttcctc
    34381 gcttccgatc aggcgaaata catcaccggt caaacgctgc acgtcaacgg cggtatgctg
    34441 atgccctaac ggacaggctt ttcaaccatg ccgtctgaag ccctttcaga cggcatttgc
    34501 attcccaaac aaaatgaaca ctcaccacca cctgccctgc ccctgcggtt caggcacaag
    34561 ctatgccggc tgctgccgac cgcttcatct gcgccaaatc ctgccgccca ccgccgaagc
    34621 cctcatgcgt tcgcggtacg gcgcgtatgt gctgcacctt atcgactaca tcatcgctac
    34681 taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcagt ggagcagaga
    34741 agccgaatgg ctgggcttga atgtcatcgc acaccgcaac ctcggcaaac gcacgcccaa
    34801 gtcgaatttg aagcctactt ccaagacggc ggaaaccgaa cagtgcatca cgagctgtcg
    34861 gcattcgtga aaatagcgga gcaatggtat ttcatcgatc cggctgttcc gctgcctacc
    34921 atgaaacagc cctgcatttg cggttcgggc aaaaaattca aagcctgctg cggcaaatat
    34981 ctgcaacctg tcgcataaaa atgccgtctg aacgttcaga cggcattttc aacgcgcaaa
    35041 aaaaaaacca ttcataccaa gggtaagtat gaatggtcaa tacattgcgg gaaaacatct
    35101 tacttgctgc actgccgaaa agggagaaac ggcagtgtta accagcaaaa gcattgtacc
    35161 tttattaatg ttaaaaaaca ttaattttga aaatatatta acgaatatat ctggaaccta
    35221 ctggttttcc cgtatccacc cgacccagcg ttcaaacagc ttcggttcga gcgcggcgac
    35281 gaccgagcgt ttgaacacgt gttcaccact ccaaaacccg tcgccttcca aagtcgtcag
    35341 cctgccgccc gcctcctcga aaatcaacgc acccgcagca taatcccaca gcttctgccc
    35401 gccgtggaca taaacatcat aacgcccgca cgccagataa caccagtcca gcgtgctgct
    35461 gcccatactg cgtatcgtcc cgaaaggcgc gagcgtactc atgcggctgg aaagtttgcc
    35521 cgaacgcaga tatttgattt ccacgcccgc aatcgcctca ttgagttttt tatccacgag
    35581 acgcaaaggc aggcgtgtcc cattaagaaa cgcaccctgc ccgcgttcgg cataaaaaca
    35641 ttcgccgctg accggattgt agattacgcc caattcggcg cgcccgttgc ggacaaacgc
    35701 caccgacacg gcaaaatgcg gcagcccgtt gacaaaattg ttcgtcccgt ctatcggatc
    35761 gacaatccac agcccctttt cccccgaata ttgttcccac aaagccgact gttcctgccg
    35821 cgacatttcc tcgcccaaca tcgggcaatc gatcagaagc ggcaacgcgg cggcaaatgc
    35881 cgtctgcgcg gcaatgtccg cctcgctcaa catcgaaccg tcttccttgc ggcgggacgg
    35941 cgtattcaaa aaacgcggca taatttcggt ttgcgcgata tggcgcacga ctttctgcaa
    36001 acggtgtaac acttcctact gtcctcatat tttgaacttg cggcgcgcga acgtataatg
    36061 tccgtttcca tcacgccgct gcgacggatt ataaccgtcc gaaccgccaa aaactatgcc
    36121 cagattctac ctgcccgaaa acctttccgt cggacaaacc gtcgccctgc ccgacaacat
    36181 cgtccgccac ctcaacgtcc tgcgcgtccg ccccaacgaa aacatcaccc tcttcgacgg
    36241 caaaggcaag gcacataccg cacggctgac cgttttggaa aaacaccgcg ccgaagccga
    36301 aatcctgcac gaagacacaa ccgacaacga gtccccgctc aacatcacgc tcatccaatc
    36361 catttcctcc ggcgatcgca tggatttcac cctgcaaaaa agcgtcgaac tcggcgtaac
    36421 cgccatacag cccgtcatca gcgaacgctg catcgtccgc ctcgacggag aacgcgccgc
    36481 caaacgcctc gcacgctggc aggaaatcgt catctccgcg tgcgaacaaa gcggcagaaa
    36541 caccgttccc cccgtactgc ccatcatcgg ctaccgtgaa gcactcgaca aaatgccgtc
    36601 tgaaaacacc aggctgatta tgagcatcaa ccgcgcccgc aaactcggcg acatccgcca
    36661 cccgtccggc gcaatcgtct ttatggtcgg gcccgaaggc ggctggacag aacaggaaga
    36721 acaacaggca tttgaagccg gctttcaggc ggttacactc ggaaaacgga ttttacgcac
    36781 agaaaccgcc ccactcgccg ccatcgccgc gatgcagacg ctttggggcg attttaccta
    36841 gcagatgaaa aaacacggga ttttatccga aaaagcaaca atccccccgc cgtcat
//
LOCUS       DAXDZG010000013        35940 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXDZG010000013 DAXDZG010000000
VERSION     DAXDZG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35940
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.013"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            319..1776
                     /locus_tag="ACFB78_000556"
     CDS             319..1776
                     /locus_tag="ACFB78_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1268612.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2108..2467)
                     /gene="rnpB"
                     /locus_tag="ACFB78_000557"
     ncRNA           complement(2108..2467)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFB78_000557"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2546..3649)
                     /gene="prfB"
                     /locus_tag="ACFB78_000558"
     CDS             complement(2546..3649)
                     /gene="prfB"
                     /locus_tag="ACFB78_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC1268613.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(3752..4645)
                     /locus_tag="ACFB78_000559"
     CDS             complement(3752..4645)
                     /locus_tag="ACFB78_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC1268614.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            4630..4914
                     /locus_tag="ACFB78_000560"
     CDS             4630..4914
                     /locus_tag="ACFB78_000560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268615.1"
                     /translation="MKTLPYFPFLYILLPVKKKGGGLLFLTPPLTAKLKGAFYIIIKI
                     NIFFFSFTEIVFEGIPSKAGIISQHRRYPNIPPFSLSFRQNTFFLYSLTC"
     gene            4994..5443
                     /locus_tag="ACFB78_000561"
     CDS             4994..5443
                     /locus_tag="ACFB78_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC1268616.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPRCRLKQD"
     gene            5517..5825
                     /locus_tag="ACFB78_000562"
     CDS             5517..5825
                     /locus_tag="ACFB78_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268617.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAADKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            5943..6785
                     /locus_tag="ACFB78_000563"
     CDS             5943..6785
                     /locus_tag="ACFB78_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC1268618.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELTYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            6775..7521
                     /locus_tag="ACFB78_000564"
     CDS             6775..7521
                     /locus_tag="ACFB78_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC1268619.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSPPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            7543..8130
                     /locus_tag="ACFB78_000565"
     CDS             7543..8130
                     /locus_tag="ACFB78_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC1268620.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8136..8315
                     /locus_tag="ACFB78_000566"
     CDS             8136..8315
                     /locus_tag="ACFB78_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC1268621.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8547..8987
                     /locus_tag="ACFB78_000567"
     CDS             8547..8987
                     /locus_tag="ACFB78_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC1268622.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(9139..9654)
                     /locus_tag="ACFB78_000568"
     CDS             complement(9139..9654)
                     /locus_tag="ACFB78_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268623.1"
                     /translation="MKTFLYTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(9669..10271)
                     /locus_tag="ACFB78_000569"
     CDS             complement(9669..10271)
                     /locus_tag="ACFB78_000569"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC1268624.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPSENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(10305..11576)
                     /gene="purD"
                     /locus_tag="ACFB78_000570"
     CDS             complement(10305..11576)
                     /gene="purD"
                     /locus_tag="ACFB78_000570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC1268625.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            11746..12051
                     /locus_tag="ACFB78_000571"
     CDS             11746..12051
                     /locus_tag="ACFB78_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC1268626.1"
                     /translation="MDEIQSKYREWCHLLLQLEENIRRWKYAVALIRDMDNFYTHEYQ
                     VRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSVDTLDRYTQEN"
     gene            complement(12103..12714)
                     /locus_tag="ACFB78_000572"
     CDS             complement(12103..12714)
                     /locus_tag="ACFB78_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC1268627.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(12777..13712)
                     /locus_tag="ACFB78_000573"
     CDS             complement(12777..13712)
                     /locus_tag="ACFB78_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC1268628.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            complement(13723..14472)
                     /locus_tag="ACFB78_000574"
     CDS             complement(13723..14472)
                     /locus_tag="ACFB78_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC1268629.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(14698..15666)
                     /gene="waaC"
                     /locus_tag="ACFB78_000575"
     CDS             complement(14698..15666)
                     /gene="waaC"
                     /locus_tag="ACFB78_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC1268630.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            complement(15703..16339)
                     /locus_tag="ACFB78_000576"
                     /pseudo
     CDS             complement(15703..16339)
                     /locus_tag="ACFB78_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
     gene            complement(16352..16534)
                     /locus_tag="ACFB78_000577"
     CDS             complement(16352..16534)
                     /locus_tag="ACFB78_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268631.1"
                     /translation="MRSEILGNKIGLQLLARSTLGIAKMEEVDMTYHIHLDGLCSLNK
                     EGKSFVIRKIENDDLK"
     gene            16799..17479
                     /gene="tsaA"
                     /locus_tag="ACFB78_000578"
     CDS             16799..17479
                     /gene="tsaA"
                     /locus_tag="ACFB78_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC1268632.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            complement(17631..18635)
                     /gene="gap"
                     /locus_tag="ACFB78_000579"
     CDS             complement(17631..18635)
                     /gene="gap"
                     /locus_tag="ACFB78_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC1268633.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            19066..21660
                     /gene="mutS"
                     /locus_tag="ACFB78_000580"
     CDS             19066..21660
                     /gene="mutS"
                     /locus_tag="ACFB78_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC1268634.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(21936..22427)
                     /locus_tag="ACFB78_000581"
     CDS             complement(21936..22427)
                     /locus_tag="ACFB78_000581"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1268635.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(22431..22826)
                     /locus_tag="ACFB78_000582"
     CDS             complement(22431..22826)
                     /locus_tag="ACFB78_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268636.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            complement(22847..24241)
                     /gene="gltX"
                     /locus_tag="ACFB78_000583"
     CDS             complement(22847..24241)
                     /gene="gltX"
                     /locus_tag="ACFB78_000583"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC1268637.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            complement(24357..25013)
                     /locus_tag="ACFB78_000584"
     CDS             complement(24357..25013)
                     /locus_tag="ACFB78_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC1268638.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(25010..25363)
                     /gene="arsC"
                     /locus_tag="ACFB78_000585"
     CDS             complement(25010..25363)
                     /gene="arsC"
                     /locus_tag="ACFB78_000585"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC1268639.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            25487..25969
                     /locus_tag="ACFB78_000586"
     CDS             25487..25969
                     /locus_tag="ACFB78_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC1268640.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            26163..26813
                     /locus_tag="ACFB78_000587"
     CDS             26163..26813
                     /locus_tag="ACFB78_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC1268641.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            26810..27727
                     /gene="ftsX"
                     /locus_tag="ACFB78_000588"
     CDS             26810..27727
                     /gene="ftsX"
                     /locus_tag="ACFB78_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC1268642.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            27820..28068
                     /locus_tag="ACFB78_000589"
     CDS             27820..28068
                     /locus_tag="ACFB78_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC1268643.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(28120..29313)
                     /locus_tag="ACFB78_000590"
     CDS             complement(28120..29313)
                     /locus_tag="ACFB78_000590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC1268644.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(29394..30647)
                     /gene="murA"
                     /locus_tag="ACFB78_000591"
     CDS             complement(29394..30647)
                     /gene="murA"
                     /locus_tag="ACFB78_000591"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC1268645.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            complement(30868..30943)
                     /locus_tag="ACFB78_000592"
     tRNA            complement(30868..30943)
                     /locus_tag="ACFB78_000592"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30908..30910),aa:Lys,seq:ttt)
     gene            complement(31012..31986)
                     /locus_tag="ACFB78_000593"
     CDS             complement(31012..31986)
                     /locus_tag="ACFB78_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC1268646.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            complement(32019..32468)
                     /locus_tag="ACFB78_000594"
     CDS             complement(32019..32468)
                     /locus_tag="ACFB78_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC1268647.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            complement(32509..33780)
                     /gene="waaA"
                     /locus_tag="ACFB78_000595"
     CDS             complement(32509..33780)
                     /gene="waaA"
                     /locus_tag="ACFB78_000595"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC1268648.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(33843..35291)
                     /gene="gnd"
                     /locus_tag="ACFB78_000596"
     CDS             complement(33843..35291)
                     /gene="gnd"
                     /locus_tag="ACFB78_000596"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC1268649.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            complement(35371..35634)
                     /locus_tag="ACFB78_000597"
     CDS             complement(35371..35634)
                     /locus_tag="ACFB78_000597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268650.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
BASE COUNT         8876 a        10115 c         8254 g         8695 t
ORIGIN      
        1 aagttaatcc accataatgc gcccttcggc gtggcggata tataaggaag tgattttcca
       61 tctaagtaaa aaccgcccta tcggataagc cattgacaga aaaggattta cccgcgccgt
      121 atcggaacac gtcctctaaa atacaatcca ttgaattgaa acaaatataa aaacatccgc
      181 ccgcgaaaaa cggcagcgcg tcgtttgaca aagaatgaaa atatcggtta aaaaccgata
      241 ttttcataca aaaaaacacc gccgccgtcc gcatccgttt cagacggtat tgagagaaaa
      301 tcttttagga gaacctttat gtcccggcat cccgcccgca ccggagaaaa aacatttttc
      361 ggccacccct tccagctttc caccctcttc catatcgaat tgtgggaacg tttttcattt
      421 tacggaatgc agggcatcct gctgatttac ctctactaca ccgccgacaa aggcggcttg
      481 ggcatagaca aaaccctcgc aggcggcatt gtcggcgcat acagcggcag cgtgtacctg
      541 tccaccattt tgggcgcgtg gtttgccgac cgcgtatggg gtgcggaaaa aaccctcttc
      601 ctctcgggca tcgtcgtaat gctcgggcac atcgtccttg ccgccgcacc gggcctgtac
      661 ggccttctaa tcgggctgat attcatcgca ttgggcagcg gcggcgtgaa atctacggcc
      721 agttctatgg tgggcgcatt atacgaacag gacgaaatgc gcccgctgcg cgatgcggga
      781 ttttccattt tctacatcgc catcaatatc ggcggcttct taggcccctt attgaccgga
      841 ctgcttcaag aaaacatcgg tttccattat ggtttcggcg cggcggcggt cggtatggca
      901 ttcggcttgt ggcgttattc cttgggacgt aaaaacctgc cccaccccac cgtcccccat
      961 ccgctttcaa aaggacaggg caaaactgcg gccgccgtcg gcatcaccct catcgccgca
     1021 cttgcaaccg ccatcaaaac cgggcttgtc aacctcgaca atttctccgg catcctctta
     1081 tctaccgtca tccttgccgt catcgcctat ttcgcccgcc tgctgaccaa cccccgcgtc
     1141 agttccgaca acaaacggca catcatcgcc tacatcccgc ttttcctgac catctgtatg
     1201 ttttgggccg tctggtttca gatttacacc gtggcaaccg tctatttcga cgaaaccgtc
     1261 aaccgcacca tcggttcgtt taccgtaccc gtcgcctgga aagattctat gcaaagcctg
     1321 tgggtcatcc tgttttccgg actcatggcg gcaatgtgga caaaaatggg gcgcaaacaa
     1381 cccaaaaccc cgctgaaatt cgctatggcg gtatttgtta ccggcgcgtc gtttttggga
     1441 ttcgttccct ttatttcctc cggaacgccg atgcctattg cggttttcgc actgatcgtc
     1501 ctcgccatca cgataggcga actgatgatt tccccgattg cgctgtccat ctccaccaaa
     1561 atcgcaccgc ctttattcaa aacccaaatg gtcgccctta atttccttgc cttttcatta
     1621 ggcttcactt tgggcggcgt attgtttgaa aaaggctatc aggcgggcga cgaaatcggc
     1681 ttctaccggc tgctgttcta catcggcgca gccacaggct tcctgctgct cctgctcgtc
     1741 cccaaattga acaaaatgct cgaaggcaca gactaagtcc tgccccgatg cgccgatacc
     1801 gtctgaaccc ttcagacggc atttttccgc ataatgaaac caaaccgttt ccacccgaca
     1861 ggacaggctt tccccccccc caaccggaag gcagcctgcc gattgtcatt taaatacccg
     1921 cctgattccg tctgtttgca agggaaatag ccgtttattt ccgcttgggc ggaaaccggt
     1981 cctattgagt aaaaggcatt ttgtccgact catcagggct gcatcaacgg aatataaaaa
     2041 cacagcctaa ataaaaatgc cgtccgaacc gtatttcagg tttcagacgg catttgcgtg
     2101 tcggatgcac accggacagg cggtaagccg ggttctgtct cggacagtca ttcctctagg
     2161 cataccgttg ccggtatgct caagcaacct acccgaacgc tcggcgggca gcgtcattgc
     2221 gttctgtttg gtcttgctcc gaatggggtt tggcctgccg catattgtta ccaaatgcgc
     2281 ggtgcgccct taccgcacct tttcaccctt gcctgtgctg ccaaagcagc catcggcggt
     2341 tttgctttct gttccacttt ccgtcgcgtt accgcgcccg gccgttaacc ggcattctac
     2401 cctgcggagc ccggactttc ctccccgtat gccttacgcg atacgcggcg actgtctgcc
     2461 cgtcccgtgt gcggcgcgga ttataacacg aaacgcaaaa atgccgtctg aaacggtaca
     2521 ggtttcagac ggcatacagc ctaaactaca caccctgttt caggctggct tcgataaagc
     2581 cgtccaagtc gccgtccaat acggctttgg tgttgccgac ttcgtagcct gtacgcaagt
     2641 ctttgatgcg tgaggaatcc aaaacatacg aacggatttg gctgccccaa cccacatcgg
     2701 atttaccttc ttccaatgcc tgtttctctt cgttgcgttt gcgcatttcc aattcataca
     2761 gtttggattt caacatttcc atcgcggccg ctttgttggc gtgttgcgaa cggtcgtttt
     2821 gacattgcac cacaatcccc gtcggctcgt gggtaatacg cacggcggag tcggttttgt
     2881 tgatgtgctg accgcccgcg cccgatgcac ggtaggtgtc gatgcgcaaa tcggcggggt
     2941 tgatttcgat ttcgatggaa tcgtcgattt cagggtaaac gaacacggag gcaaacgagg
     3001 tatggcgttt gttgttcgaa tcaaacggcg agtagcgaac caagcggtga acgccggttt
     3061 cggtacgcag caaaccataa gcgtattcgc cttccactcg gatggtggcg cggttgatgc
     3121 ctgcgatttc gccgtcgtct tcctcaagga tttcgatttt gaagcctttg cgctcggcat
     3181 agcggctgta catacggaac agcatccccg cccagtcttc cgcttcggta ccgcccgcgc
     3241 cggcggtgat gtcgataaag cagttgttcg ggtcggcggg ctggttgaac atccgtttga
     3301 actccaaatc cgccatctgt ttttccagcc ccgccacgtc ttccttcacg gcggcaaaac
     3361 cttcttcgtc gttttcttcg acggccattt caatcagcat acggttgtct tcgatacccg
     3421 aagcgatgtt gtcgagcgtc agcacgatgc cttcaaggat tttgctctct ttgccgattt
     3481 cttgggcacg tttcgggtcg ttccaaagct cggggtcttc ggaaagcccg atgacttctt
     3541 ccaatcggtc tttcttgccc tgataatcca tataaacgcg gatgtcttcg ctacgcttct
     3601 ccaaatcatt cagggtgttg ttgagctggt tgattacttc ggcttccatg attctttcgt
     3661 tctttcaaaa ttttaggggc gtattgtacg ggattcgggt atttttttct atggataaag
     3721 ccttctggaa atacgttcgg acggcatagc gtcaataacg gtatgccgcc agtttgcgtt
     3781 tgatttcagg caatgcggca cgtgctgcct cctcgcccaa ccggatggcg cgctttttct
     3841 gatcaaatcc gccgactgca cccaaatcca aaacctgcgg tttgataacc acatccgcct
     3901 gccccaactc gttttgcaac acggaaacgc tcatcacgtt cagcgtctga tcgagataag
     3961 agaagaaacc ttgaccgaca tttttgctcg gacgtgcgga aatatcgacg gcaatcacga
     4021 aattcgcccc ctgccgccga gcggcactga cgggcacggg ctgcgacaga ccgccgtcaa
     4081 catatttgtg cctgccgatg atgactggct ggaacacatt gggaatggcg gcggaagcac
     4141 gaaccgcctg cccggcattc ccttgattga aagcgacggc cttgccggtt tcaaaatcag
     4201 tggcaacggc ggcaaatttg atgggaaact gctgaatctg cctgccgccg acttttcggt
     4261 tgatgtaatt ttgcagcttt tcgcctttga taaaaccact ggtggacaag gttaaatcga
     4321 ctaaatcggt tttacctaaa atctcggctt ccaattcgag gcggtcgggc gacatacccg
     4381 atgccaaaag gctgccgact atcgaacctg ccgatgtgcc ggtaaccacc ttcacaggaa
     4441 taccgttttc tttcaaaacc ttaacaattc ctatatgggc aaatccttta gatgcgccgc
     4501 caccgagtgc caaagcgacc actgcggcgg gtttggcggt ttgcaccggc ttgcgggcgg
     4561 cattgtttcc cgccgtaccg caggcggcaa gcaacgcggc ggcggcgatt gccaaaaatg
     4621 atctgatttt tgaaaacgtt accatatttt ccattccttt atatattgct ccccgtcaaa
     4681 aaaaaagggg ggggattgct tttcttaacc ccccctttga cagccaagct aaagggggct
     4741 ttttatatca tcatcaaaat taatattttc tttttttcct ttacggaaat tgtatttgaa
     4801 ggcataccgt ccaaggcggg aattatctca caacaccgcc gttatccaaa tatcccgcct
     4861 ttttcccttt ctttccgtca aaatactttc tttttatatt cattaacttg ttaaattatt
     4921 agctgacggg tgtcagtttt tccgacaaaa tccgtctaac ggggtatcaa caggaacaaa
     4981 aacaggaaca cttatgaaaa tcggaacaac ttggcagacg gcatccgcta tgctggtttt
     5041 gcgtctgttt gccgcatatg aatttttgga atcgggtttg caaaaatgga acggggagaa
     5101 ttggttttcc gaaatcaacg atcagtttcc attcccgttc aacttgctgc cggacgcgtt
     5161 aaactggaat ttcgccatgt atgcggagct tttgctgccc gtactgttgc ttttgggttt
     5221 ggcaacgcgc ctgtcggcat tggggctgat ggtcgttacc gccgtcgctt gggctgcggt
     5281 tcacgccggt tcgggttaca atgtctgcga caacggttat aaaatggctt taatttatat
     5341 cgtggtatta atcccgctgc ttttccaggg tgcgggcgga tggtcgctgg atacgctgct
     5401 gaaaaaacgg ttttgccccc gatgccgtct gaaacaagat tgattcagtc gtggaatccg
     5461 actttaaaca ttccaacctt atctcgttaa cttgatattc tgaaaaggaa atagaaatga
     5521 acaaaaacat tgctgccgca ctcgccggtg ctttatccct gtctttggcc gccggcgccg
     5581 ttgccgccga caaaccggca agcaacgcaa caggcgttca aaaatccgcc caaggctctt
     5641 gcggcgcatc caaatctgcc gaaggttcgt gcggcgcggc tgcttctaaa gcaggcgaag
     5701 gcaaatgcgg cgagggcaaa tgcggtgcaa ctgtaaaaaa agcccacaaa cacaccaaag
     5761 catctaaagc caaagccaaa tctgccgaag gcaaatgcgg cgaaggcaaa tgcggttcta
     5821 aataatcccg ccccttcaaa ccaagccgcg tttttcagta aaatgcggct tttttaacgg
     5881 caaacaaaga ttttttttaa caagcacatc attcttttgt gccatccgaa ccgggtaaaa
     5941 atatgattca acacgcaggc ttgggctacc gccgcgattt ggcggaagac tttctctcgc
     6001 tttccgaaaa cagcccgata tgctttatcg aagccgcacc ggaaaactgg ctgaaaatgg
     6061 gcggcagggc gcgcaaacag tttgaccgtg tggcggaacg gctgccgctg gcgttgcacg
     6121 gattatctat gtcgctgggc ggacaagccc cgctggatac cgatttgata gacggcatca
     6181 aagaaatgat gtgccgttat gactgcacgt ttttctccga tcatttgagc tactgccacg
     6241 acggcggtca tctttacgat ttgttgccgc tgcctttcac tgaagaaatg gtgcatcata
     6301 cggcgcggcg tatccgcgaa gtacaagacc gtttgggctg ccgcatcgcc gtggaaaaca
     6361 cgtcctacta cctgcattct ccgcttgccg agatgaacga ggtcgagttc ctcaacgcgg
     6421 ttgcccgtga agccgactgc ggtattcatt tggatgtgaa caatatttac gtcaacgccg
     6481 tcaatcacgg cctgctgtcg cctgaggcct ttttagagaa tgtggacgca gggcgcgtgt
     6541 gctacatcca catcgccgga cacgatgccg aaacccccga gcttttgatt gatacccacg
     6601 gcgcggcggt tttaccgacc gtttgggact tgctcgaact cacctacacc aagcttccca
     6661 ccatccctcc caccctgttg gaacgcgatt tcaatttccc accttttgcc gaactcgaag
     6721 ccgaagttgc caaaattgcc gattatcaaa cgcgtgccgg aaaggaatac cgccgtgcag
     6781 cctgaaacct ccgcccaata ccagcaccgt ttctcccaag ccatacgcgg gggcgaagcc
     6841 gcagacggtc tgccgcaaga ccgattgaac gtctatatcc gcctgatacg caacaatatc
     6901 cacagcttta tcgaccgctg ctataccgaa acgcggcaat actttgacag caaagaatgg
     6961 agccgtctga aagaaggttt cgtccgcgac gcgcgtgccc aaacgcccta ttttcaagaa
     7021 atccccggcg agttcctaca atattgccaa agcccgccgc tttcagacgg cattttggcg
     7081 ctgatggatt ttgaatatac ccaattgctg gcagaagttg cacaaattcc ggatattccc
     7141 gacattcatt attcaaatga cagcaaatac acgccctccc ctgcggcctt tatccggcaa
     7201 tatcgatacg atgttaccca tgatttgcag gaagcggaaa cagccttgtt aatatggcga
     7261 aacgccgaag atgatgtgat gtaccaaaca ttggacggct tcgatatgat gctgctggag
     7321 ataatgggtt cctccgcgct ttcgtttgac accctcgccc aaacccttgt cgaatttatg
     7381 cctaaagccg ataattggaa aaatattttg cttgggaaat ggtcaggctg gattgaacaa
     7441 aggattatca tcccctcctt gtccgccata tccgaaaata tggaaggcaa ttccccgagc
     7501 caaaaccatc tatccgcata aaattacctt gttcccgata ctatgccgct acccgacctg
     7561 accgatgccg aattaatgga gtcgcgcaaa ctgcttctgc attttgcgcg gctccagttg
     7621 cccgaccacc ctgatttggc tgaagattta gtgcaggaaa cattgctgtc cgcatacagc
     7681 gcaggcgaca gttttcaagg cagggcactt gtcaacagct ggctttttgc catattgaaa
     7741 aacaaaatta ttgacgcatt acgtcaaatc gggaggcaga ggaaagtctt taccgcactg
     7801 gatgacgagc tgctggatga agcatttgaa agccattttt cccaaaacgg gcattggacg
     7861 ccggaagggc agccgcaaca ttggaacact ccggaaaaat cattaaataa caacgaattc
     7921 caaaaaattc tgcaaagctg cctatacaac ctgcctgaaa acaccgcacg ggtattcacc
     7981 ctgaaggaaa tactcggttt ttcatccaac gaaatacaac aaatgtgcgg tatcagcacg
     8041 tccaactacc acaccattat gcaccgcgcc cgagaatcat tgcgccaatg cctgcaaatc
     8101 aaatggttca accaagaaaa cccgaagtaa acgttatgaa aaaatgccgc gatattgccc
     8161 tgcttctttc caaacatcag gaccgggaaa ccaccccggg cgagaagatt tccatatata
     8221 tgcacctgct gttctgcccg cattgccggg aatataaaag acagcttcaa accatcaaaa
     8281 tatcactggc aaaaacaacc agaacttcaa aataaatgcc gtctgaaagg gcttcagacg
     8341 gcataagctg acggaaacaa atcaaaccga tttactgtta tcggcagttc atccataata
     8401 cactcttcaa aagcagcata ttcctccata cggaatatat aaatacgtaa aacacgaagg
     8461 ctgcatcaat ttcccatatt tgcctcattt cacagacggc attacccctc ccgcccaacc
     8521 cgttctttct gaatgagcgg atttcaatga ttaaggaaac cctaatgcgc ccaatcttcc
     8581 tatctttcgt tttactccct attttgataa ccgcctgcag cacaccggac aagtctgccc
     8641 gatgggaaaa tatcggcaca atctcaaacg gcaatattca tacatatatt aataaagaca
     8701 gtgtgagaaa aaacggaaat ctgatgattt tccaagataa aaaagttgtt accaatctga
     8761 aacaagaacg ttttgccaac acccccgcat acaagactgc cattgccgag tgggaaatcc
     8821 actgcaacaa caaaacatac cgcttaagtt cgctacagtt atttgataca aaaaacacgg
     8881 aaatttccac acaaaactac acagcctctt ccctccgccc gatgagcatc ctgtccggga
     8941 cattaactga aaaacaatat gaaaccgtat gcgggaaaaa actctgattt caacttatac
     9001 acaaatttat ccataagcct tattataaaa atgccgtctg gaatactgaa atatcagcat
     9061 ttcagacgac attttgccat tccctgaaat tatccacatt atttttttaa accggcttcc
     9121 atccgaaata taaacccgct atacaatctc actattcaaa cgtaaattgt tccattgata
     9181 cacaaaactg cttgccctca taattttgat aaagcatttt ttacattccc ggctccgtcc
     9241 cgtaaccaac acagcctcta aaaaataatc gcttgttttg acaccgatac attcatacag
     9301 atacagaaat tcaagaaaat agccttgtgc attgatcatt tcaagcaatt cagaaaaatc
     9361 aagaaatttt ctgactgtaa acaaacgttt ccctaaaaag acgatgtctt caaaaatatc
     9421 gaccaaatag tcgtcttcgg cgtttctatt ttcatgggtt aaaacaacac gggaaaaatc
     9481 tgttttcaga tgcttgcccg cttgattgtt cggattattg tccagaacga caaaaccgtc
     9541 ctcaaaatca aagcagacgt tgcgtccttc tacctttatc gccgtgcgat aacaatcatg
     9601 tagagaaata ccatccaaaa aattttttct ttgtgtatac aaaaaagttt tcactcaagc
     9661 atccatatct aacgcaaacg tttacctgtt tccccatcga taatctgact cggcgatttc
     9721 tgcctgccga ttctcccacc gacaatccac acatcgcgtc cgaactgcct tctgacttcc
     9781 ctctccgtcc gacacgcgcg tttacccgcc cgattacatg aagtggaaac caaaggcata
     9841 cccaatgcct gacacaaacg ccttgcacct gtatgatcgg gcactctgac cgcaagcttg
     9901 ctgcgcctct tcccgcgcaa cgcaggtaat acgccagatt tagccaacaa taggaaagtc
     9961 ttaggagcag gccattcgtt tctaagcatg gtttgaatgt tttcagacgg catttgaagt
    10021 aaaggctgca attgttcaag ttgattcccg atgacaatca tacccttgtg ttgcggcctt
    10081 tttttcaaat gcgccaactt accgagtgct ttggctaatg tcggaagaca ccccaagcca
    10141 taacaagatt cggtcggata agcaaccaaa ccgccttttt tcaaataaac gcttaactta
    10201 cgttgcgctg atgctgcgat aattctcgaa aataacataa tataaaatgc cgtctgaagc
    10261 gcatcagtca tacttggctt cagacggcat catcctcttt ctaattaacg gttaatcgct
    10321 ttatcggcaa tgtctttacg gtattgcatc ccgtcgaagc tgattttttc caacgcgcca
    10381 tacgccttgg ctttcgcttg cgccacatcg tcgcccaatc ctacaacaca caatatgcgc
    10441 ccgccgttgg tcaacacgtc gcctttctcg tttaccgttg tgccggcatg gaatactttg
    10501 ccgattcggt tgacgtcatc caaaccggaa ataacatcgc ctttcttggg tgtttcgggg
    10561 taattttgcg ccgccagtac cacacccacg gcagtttgcg gattccattc cgctttgacg
    10621 ctgtccagtc tgccgtctat tgccgcttca accaaatccg ccaaatcgct gtttagacgg
    10681 ctcataatcg gctgggtttc gggatcgccg aaacggcagt taaactcaat cgtatagggt
    10741 gcaccgcttt gatcgatcat caaacctgcg tacaaaaagc cggtgaactc gtgcccctcc
    10801 gctttcatac ccgctacggt cggcaaaata atctcgttca tcgcacgctc gtacacagca
    10861 ggcgttacca caggcgcggg actgtacgca cccataccgc ccgtattcgg gcctttatct
    10921 ccgtctaaaa gacgtttgtg gtctcgactg gtcgccatag gtaatacatg attgccatca
    10981 accataacga taaagctcgc ttcttcgcct tgcagaaaat cttcaatcac aacgcgttcg
    11041 ccggcattgc ccattttatt gcccaacaac atatcgtcaa tcgcagcatg cgcttcatcc
    11101 aaagtcattg ccacaatcac gcctttacct gccaccaaac catcggcttt gataacgata
    11161 ggcgcacctt tctgattgac gtaatcatgt gcggcatcgg cattttcaaa ggtttggtat
    11221 tgcgcggtcg gaatattgta tttcaccata aatgctttgg cgaaatcttt agaactttcc
    11281 aactgcgccg catattgtgt cggaccaaat attttcagcc ctgcggcacg aaaatcatcc
    11341 acaatacctg ccgccaaagg cgcttcaggg ccgacgacgg taaaaacaat attttcttta
    11401 cggcagaatt caatcaaatc ctgatacgcg gtcaaggcaa tgttttggag cttggattca
    11461 atcgccgtac cggcattacc gggcgcaaca aatactgttt ccactttggg cgactgcgcc
    11521 aacttccaag ccagcgcgtg ttcgcgaccg ccattaccga taaccagcag tttcatgcca
    11581 tctccttgac aaatatgtac ttttaacgaa aactcgatac aaaggaactt ttgtcccatc
    11641 tgaagaaatt ttagtagaat caaacaaaag accacttcat tccactctgc aacctattca
    11701 acttattcac aaattaaaaa ataacaagca aacacgcaaa aacgtattga tgaaatccaa
    11761 agcaaatacc gcgaatggtg tcatttacta ctgcaactgg aagaaaacat ccgccgttgg
    11821 aaatatgccg tcgctttaat tcgcgatatg gacaatttct atacccacga gtatcaggtg
    11881 cgccatcaga ctattgaaga cggggtagaa ctgaatttgc aaacagaagg cgaatatagc
    11941 attatgagtg aagatgcatt atggaacgcg ccgggcgaat tccatcaatt ggcttggcta
    12001 tatttatgct ccagcgtcga taccttagac agatatacac aagaaaatta atcagcgaag
    12061 aggttgcctg aaataccatt acaaagcatt tcatacaacc tttcattcaa aaggcttttc
    12121 cgtatttact tcaacctgcc gtgtattctt ccaagccgca acacaggcct cataatttgc
    12181 caacgacaaa cttaccgtca atcggcaatc caactgcaaa tcctgctcca atatatccgc
    12241 ctgatattgt ttggcaatgc atattgcttc attcaaaaac ggatactcac atttcaacca
    12301 aacggttttt tcaatattct tttcaaccac ttccgcgatc gccaacgcct gagccgttgc
    12361 ctctttgtat gcgtgaatca agccgggaac gcccaataaa gcaccaccga aatagcggac
    12421 gaccacgacc aaaacatcag tgatacccac cgaatcaatc tgtcccaaaa ttggccgccc
    12481 agcacttcct ggtggctctc catcatcgtt gccgcgaaac tgcatcccgt tcacacctaa
    12541 acgataggca tagcaccaat gccgcgcttt ataatgctct tcctttaaca gattgaggta
    12601 ttttttcaca tcagccaatg tccgaatcgg ataggcaaat gcaataaaac ggctgccttt
    12661 atctttaaac tcagcctgcg tcggggaagt aatggtttta taagtcgtaa tcatgctgaa
    12721 atgttttcag acgacctcat taataacaaa gtcgcctgaa agtttcacgt gaaacatcaa
    12781 tttttcaata attctattaa ttgtggaaca atttcaaata aatcgccaac caatccgtaa
    12841 tcggctacat tgaaaatcgg cgcatcggca tctttgttga ttgcaacaat caccttactg
    12901 tcctgcatac cggcaacgtg ttgaatcgca cctgaaatac cgattgcaaa atagagctgc
    12961 ggcgcaacca ccttaccggt ttgtccgact tgagcatcgt ttggtgcata ctcggcatca
    13021 accgccgcac gggatgcacc gattgccgca cctaaaacat ccgccaacgg tgtcagcact
    13081 tcactgaatt tttccacact acccaacgca cgaccaccgg aaacaatcac ttttgcctga
    13141 gtcagttcgg gacgatcgga atgggaaagt tgacggttaa caaaacgact caggttttgg
    13201 gcaggggttg ttttaacatt aattacctca gcattaccac cttgcgccgc cactgcgtca
    13261 aaagccgtca cacggaaggt cagcaccaat ttttctgaat cggcttgcac ggtttcaaat
    13321 gcattacctg cataaatcgg acgtacaaaa gtcgtgttat ctacaatctc ggtcaaatca
    13381 gaaatttgcg gtacgtctaa taaggcggct acgcggggca aaaggttttt accgaatgtg
    13441 gttgccgttg ctgcaacata gcggtaatcc gccgccaatt tgacaaccag cggagccaac
    13501 tcttcagcca aaccttcagc ataatgagca gcatctgcaa ccaaaacttt ttccactccc
    13561 gctacttgct tcgcgaattc cacgacggac gatgcgccgt ttccgacaac caataaatcg
    13621 accttgccca gtttggcggc agcggcaaca gcatgcaaag tggcaggatt caactgtttg
    13681 ttgtcgtgtt cggcaataat caatacgctc atttcagcct cctcaaatca ctttggcttc
    13741 gtttttcaat ttttcaacca attcggcaac gcttgctact tttacgcctg cctgacgcgc
    13801 cttaggttcg gcaaatttca ccgttttcag acgaggcgaa atatcggtag ccaaatcggc
    13861 agaatccagt ttttccagag gttttttctt tgccgccata atattgggga gtttgacaaa
    13921 gcgcggctcg ttcaaacgca aatctgcgct gatgactgca ggcagtttca acgcaatcgt
    13981 ttcttcgccg ccatcaattt cgcgcacaat ctgtacttca tcaccttcaa tttgtacttt
    14041 tgaaacaaat gtaccttgcg ccgcattcaa caaagctgcc agcatttgcg ccacttgatt
    14101 ggcatcgtca tcaatcgctt gtttgcccaa aaagaaaatt tgcggatttt ctttgtccgc
    14161 aacagctttc agcagtttgg caactgccag cggctctaat ttcacatcgg tttcaatatg
    14221 aatggcacgg tcggcaccca ttgccaaagc ggtacgcaag gtttcttcgc atttcttttc
    14281 acccaaagaa accgctacaa tttcgcttac tttcccggct tctttcaaac ggacggcttc
    14341 ttccacagcg atttcatcaa acgggttcat cgacattttg acattgccga tgtccacatc
    14401 cgaaccatcg gctttcacac ggactttgac gttgtagtcc actacgcgct ttactgcgac
    14461 cagtgctttc attgaattct cctaaaaaga acgctgcttt caccatccag cgaaaccaaa
    14521 ccttcttctt tataaaatca aatccgtttt ccttaaaaac gaatttattt agcaatcttt
    14581 cggataattt taccgattat accattttta aagtatttac tcaggctggc ggatatacat
    14641 tcctgtatct aataaattgg aaaatatcat gccgccatat cagttttaga cgacccttta
    14701 gcctttatct gctgaaacac aatccatcag cgtttgataa accaaatctg cggtcggaat
    14761 ctgcccgata ttgcccacat tttttgcaat agccgaaact tgaacgcctg ttttaatcgg
    14821 atcggtatcg gtataaatgc cgaccacagg tttttccaag gcatttgcca aatgcagcaa
    14881 accggtatct acaccaacta ttccaaccgc gtgtttcaac agatacgccg cctgtaataa
    14941 attcatttta gcgcacacta tggtaaacgg cagcccgtct gcaatctgtt ccgcacgcac
    15001 tttttcagcc tcgtttcccc aaggcagata aacattgcat tgttgttttt gattcagttt
    15061 ctgcagcaac tcccgccaat tttccatagg ccataactta ctgtcccgac tggtcgcgtg
    15121 caaagccgca taatacggct gcgctaaatt tttcagacgg cttgcttcag gaacggacaa
    15181 accaaatacc tgcgtctcag gcattgcata cccaaacacc tgggcaaaca gatcacggtt
    15241 gcgccaaacg gcatttctac ctttcggtac gacatacttt ttatcatacg ccaaagcagc
    15301 caccccctca cgcgcactgt gtttatccaa accataaata ggggattttg ccattttggc
    15361 gaaacacgcg cttttaatca gaccctgact gtccaataca aaatcaaatg cttcctgcag
    15421 caaagcctgt ttcagacgac ccatctcccg ccaagtttca gcctgaaaga gatgtttgcg
    15481 ccattgccgc cacttcatta catggacctt ttttacaaac ggatgcaacc gtgcaatacc
    15541 cgcaaacccg gcttcacata gccaatgcag ttctacatca gagcattgtc gcgccaaatc
    15601 ttcgattgcg ggcaaagtgt gaattaaatc acccatactg gacaaacgga caagcaaaat
    15661 tttcatatgt agacagggtg tttcacgtga aacaattata agttattgat tattaatata
    15721 tttttttaat tttttcggcg tttttcaaaa ttatcgcacc cgaagaacgc atttcctgcc
    15781 acgctgcttc aatggtatcg ggcgcaatac cccgacaagc cgcttcattc acgataacct
    15841 gccaacgacc gcctttaagt aactgcaaaa ccgttgtttt aacacaataa tccgtagcca
    15901 agccgccgac aatgaccata ttcgtatttt gacaacgcag ccactcaatc aatccagtgc
    15961 tcagtttttc ctcaatatcg tgaaaacacg cgccgtaagg atgcaattcg ggatcgacac
    16021 ctttccaaac gcaataatcg tattccttga cggaaggcag accgtccaac aattcatagc
    16081 cgcgcgtacc gaccatcgca tgagccaccc aagtcaaatc cgcatcggac aaacctgtcg
    16141 gcttcaacat atcaacaggg ttacccacaa gccatttcgc cgccatatga tgcgcatctt
    16201 tcgtcatcac acgcaaatcc gccaaagcgg cttgggcatt taactcctcg acaatcaaat
    16261 gcccctcgtt cacgggcagt tcgtcaggac acaaaggcgt aaaagttttt tgtgcatcaa
    16321 catcaatgga aacaatcatc tcattacttc ttcatttaag atcgtcattc tctattttac
    16381 gaataacaaa actcttacct tctttattta aggagcacaa tccatctagg tggatatgat
    16441 atgtcatatc cacctcctcc attttagcta tacctaaggt gctccttgct aaaagctgta
    16501 atccgatttt attacccaaa atttcagatc tcacctcctg taggtgagat ctgaaaggaa
    16561 agataacaac ctgacttggg tgtaatatac gggattccct cttcgttata acgaaccgtt
    16621 atgcttcttt tttatcaata ctccaatgcc tttttaagag cgtcttggac acaaggattc
    16681 tgtgctactt tatttcaacg cgatttaaat gccctgtatt ataacaaatt actacccgaa
    16741 agcggtaaaa ccggctgtga taagataagg tttttccaaa aaacttatcc acaatcttat
    16801 gacttatacc attaccccca tcggcaccgc ccgctcgccc tacaaacaga aattcggcat
    16861 cgcccgccag cccggtttgg tctccgccgc agaagcctgc atcgagctga atcccgaatt
    16921 taccgcagac agcgtgcgcg ggttggaaga tttcgattat gtgtggataa gttttatctt
    16981 ccacggcgta ttggatgaag gttgggcgca aatggtgcgc ccgccacggc tcggcggcaa
    17041 acaaaaaatg ggcgtgttcg ccacgcgcag cccccaccgc cccaaccatc tcggactctc
    17101 tctccttaaa ctcgaacgca tcgaaaccgg caaacccgtc cgcctctatt gcagcggctc
    17161 agacctgctg gacggcacgc cgattgtgga catcaagcct tatatcccct ttatcgaatc
    17221 caaacccgat gcagcatccg gtttcgtcag cggcaaaccc gtagagttgg aagtcgtttg
    17281 gcaggaaaac atcggcgcgg aaaatttatc tgcaaacacc aaaaacctca tcagccaaag
    17341 cattgcccaa gatccgcgcc ccgcctatca gaatattccc gaacggattt atgtgatgaa
    17401 tattgcggat tacgaagtca ggtttcaaat cgaggaaaac cgcgcgacgg ttattaatct
    17461 ttccccaacc ccgctttaaa tcgggcaaaa tccggttttg ccgcatagca gttgaacaaa
    17521 cggctgttgt ttgttcgcca taagccgcaa tatcaagtta cagataaaca atgccgtctg
    17581 aacgcaatgt gttcagacgg catttactta tccacaggtt tgttcaagcc ttagattttg
    17641 cctgcgaagt attccaaagt acggacgagt tggcaggtgt aggacatttc gttgtcgtac
    17701 caggcaacgg ttttcaccag ttgtttgccg cccacggtca tcacgcgggt ttgggttgcg
    17761 tcaaagagcg agccgtattc gatgccgaca acgtcggaag aaacgatttg atcttcgttg
    17821 tagccgtaag attcgctgga ggcggctttc atcgcggcgt tgatttcttc tttggttgca
    17881 gggcgttcga ggacggaaac caattcggtc agcgagccgg tggcaacagg gacgcgttgg
    17941 gcggagccgt cgagtttgcc gttcaattcg gggataacca gaccgatggc tttggcggcg
    18001 ccggtgctgt tgggcacgat gttgagcgcg gcggcgcggg cgcggcgcaa atcgcctttg
    18061 cggtgcggcg cgtcaagggt gttttggtcg ccggtgtagg cgtggatggt ggtcatcagg
    18121 ccttcgacta cgccgaactc tttttgcagg actgccgcca tcggggcaag gcagttggtg
    18181 gtacaggaag cggcggaaat gacagtttcg ctaccgtcca agatgtcttg gtttacgcca
    18241 tatacgacgg ttttcacatc attgccgccg ggtgcggaaa tcacgacttt gcgcgcgccg
    18301 gcacggatat gtgcttcggc tttggttttg ttggtaaaga aaccggtaca ttcgaggacg
    18361 acatccacac ccaactcgcc ccaaggcaat tcttcgggat tcggattggc gaaaactttg
    18421 atttctctgc cgtttaccac gatggcatcg tcttttaatt cggcagtacc ttggaaacgg
    18481 ccttgtgtgc tgtcgtattt gaaaaggtgc agcagcattt cggcaggggt caggtcgttg
    18541 acggcggcga cttcgatgcc gtgggctttt tcaatttgac gcaatgcgag gcggccgatg
    18601 cggccgaaac cgttaatcgc tactttgatg ctcatatttt gtctcccggt tgtgaaataa
    18661 agtccgatgc ctgtattgta ttctgaaata aaactacatt ccactattac ataaaattac
    18721 ttgcgttttg tttgacgcag gtgaatttcg ctatttcaaa aacgcgcatt caatatttga
    18781 ttttaaaatt ttaatgatga ttttgatgat tgccgacctg cttgtgtata agtagcaaat
    18841 atccaatatt ttcattacct ttttgttaaa taaatttgag tttaaggctt gccgtataag
    18901 acagataagc gtggatgttt tttgacttaa taatattctt atggataact ttgccgtttt
    18961 cctatttgtc tccacaactt tattgacagg cttacggtca gtctcattcc gtcgaagaca
    19021 aaaccttttg ctacaatacc gttttcctaa taataaggca gccccatgtc caaatccgcc
    19081 gtttccccaa tgatgcagca atacctcggc ataaaagcgc aacataccga caaactagtg
    19141 ttttaccgta tgggtgattt ttacgagctg tttttggatg acgcggtgga agcggcgaaa
    19201 ttgttggaca tcaccctgac cacacgcggg cagatggacg gcgtgccgat taaaatggca
    19261 ggcgttccgt ttcacgcggc ggaacagtat ctggcgcgcc tggtcaagtt gggcaaaagc
    19321 gtggcgattt gcgaacaggt cggcgaagtc ggcgcgggca aagggccggt ggagcgcaaa
    19381 gtcgtgcgca tcgtaacgcc cggcacgcta accgattccg cattactgga agacaaggaa
    19441 accaaccgca tcgttgccgt gtctcccgac aaaaaataca tcggtttggc gtgggcatcg
    19501 ctgcaaagcg gcgaattcaa aaccaagctg acaactgcgg ataaattgaa cgacgaactg
    19561 gcgcgcctgc aggcggcgga aattttgctg ccggacagta aaaacgcacc gcaacttcag
    19621 acggcatcgg gtgttacacg cctgaacgcg tggcagtttg ccgccgacgc gggggaaaaa
    19681 ctgctgacgg aatatttcgg ctgccaagat ttgcgcggct tcggtttgga cagcaaagaa
    19741 cacgccgttt cgattggcgc ggcaggtgcg ctgttgaact atatccgcct gacgcaaaac
    19801 ctgatgccgc aacatttgga cggcctgtcg ctcgaaaccg acagccaata tatcggcatg
    19861 gatgccgcca cgcgccgcaa tctcgaaatc acgcaaaccc tctccggtaa aaaaacgccg
    19921 accctgtttt ccatactcga cggctgcgcc acccacatgg gcagccgcct gttggcattg
    19981 tggcttcacc accctttacg caaccgcgcc cacatccgag cgcgccaaga agccgtaacc
    20041 gcgctggaaa gccaatacga acccctccaa tgccatctga agagcattgc cgacatcgaa
    20101 cgtatcgccg cccgcatcgc cgtcggcaat gcccgcccgc gcgatttggc atctttacgc
    20161 gacagtctgt tcgagttggc acaaatcgac ttgtccgcaa ccggcagcag cctgttggaa
    20221 acccttaaag ccgttttccc cgaaaccctg ccagttgccg aaaccctcaa agccgccgtg
    20281 atgcccgaac cttccgtctg gctgaaagac ggcaatgtca tcaaccacgg ttttcatccc
    20341 gaactggacg aattgcgccg cattcaaaac catggcgacg aatttttgct ggatttggaa
    20401 gccaaggaac gcgaacgtac cggtttgtcc acactcaaag tcgagttcaa ccgcgttcac
    20461 ggcttttaca ttgaattgtc caaaacccaa gccgaacaag cacctgccga ctaccaacgc
    20521 cggcaaaccc tcaaaaacgc cgaacgcttc atcacgccgg aactgaaagc ctttgaagac
    20581 aaagtgctga ctgctcaaga ccaagccctc gccttagaaa aacaactctt tgacggcgta
    20641 ttgaaaaacc ttcggacggc attgccgcag cttcaaaaag ccgccaaagc cgccgccgcg
    20701 ctggacgtgt tgtccacatt ttcagccttg gcaaaagagc ggaactttgt ccgtcccgag
    20761 tttgccgact atccggttgt ccacatcgaa aacggccgcc atcccgttgt cgaacagcag
    20821 gtacgccact tcacagccaa ccacaccgac ctcgaccaca aacaccgcct catgctgctc
    20881 accggcccca atatgggcgg caaatccacc tatatgcgcc aagtcgcgct gattgtttta
    20941 ttggcacaca ccggctgttt cgtgcctgcc gatgccgcca caatcgggcc tgtcgatcaa
    21001 atcttcaccc gcatcggcgc atcggacgac ctcgcctcca accgctccac cttcatggtc
    21061 gaaatgagcg aaaccgccta catcctgcat cacgccaccg aacaaagcat tgttttaatg
    21121 gacgaagtcg gacgtggtac ttccactttc gacggcctcg ccctcgcgca cgcaatcgcc
    21181 gaacacctgc tgcaaaaaaa caaatccttc agcctgtttg ccacccacta tttcgagctg
    21241 acctacctgc ccgaagccca cgccgccgcc gtcaatatgc acctttccgc gctcgaacag
    21301 ggacgggaca tcgtgttcct gcaccaaatc caacccggtc ccgccggaaa aagctacggc
    21361 atcgccgtcg ccaaactcgc cggcctgcct gtgcgcgcat tgaaagccgc ccaaaagcat
    21421 ttgaacggac tggaaaacca agccgccgca aaccgtcccc aactggatat tttcagtacc
    21481 atgccgtctg aaaaaggaga tgaaccgaat gtggactgct ttgtggataa agcagaggaa
    21541 aaacattttg aaggtatatt ggcagcagcc ctggaaaacc tcgatcccga cagcctgacc
    21601 ccgcgcgaag cattgtcaga actgtaccgt ctgaaagatt tgtgcaaatc cgtatcttaa
    21661 tttccgttgg cggagcagca tcaaaccata tggaaaaatc tgtggataaa ctttatggcg
    21721 gattaacaaa aatcaggaca aggcggcggg ccgcaggcag tacaaatggt acggaaccga
    21781 tccgcccggt gcttgggcac cttagggaat cgttcccttt gagccggggc ggggcaacgc
    21841 cgtaccggtt tttgttaatc cactatatct gacaggaaat ttccaaacat aaaaaatgcc
    21901 gtccgaacag ctcagacggc atctgcccat tcggcttaaa ccttatccac atccaaacgc
    21961 ataaccgtaa cccattcccc gttatggaaa tgtcgcccga caaccgccca gccgaatgat
    22021 tcataaaata tttgcacatc aggcgtataa agatacaaga actttatccc cagcgaacgc
    22081 gctgcgccta tgcagtgggc gaccagcctc ctgccaatgc cttttccgcg atattcaggt
    22141 aaaacaaaga catcgcccaa ccaatattca tactgtggaa aattttccat atcatgccgc
    22201 ttgactgcag ccgaacccaa cagggttccg gaatcatcca cagccgcaaa agccagcggc
    22261 agttcgtcat ccttcaaaca tctgccgtaa taggcacgaa tcttatccac agaagaccac
    22321 ggttcaaatc cgtgccactc ctcaaacaac gcctgaacca acctgccgat atgcccggct
    22381 ttcagccgtg taataaaaac agtgttgtcc acaaagaggg aattcatcgg tcaattcccc
    22441 cgacgctttc gttcccctgc gccgtaaacc gcattccaag catggtccaa acgcactccg
    22501 atttgcctca tctcttcagc ctgccgggct ttttgggcca ttgctgcagg aatttccgct
    22561 tccaaacggg cgatgtctgc ctgagccgcc tgcaaacgcc ggcgcgcatc ttccaaatcc
    22621 gactgcatcc cgatgatttt tccgtccaga ttgttttgct tttgcagtaa agcacggtaa
    22681 ccggattgga tgctgagcaa attgtcttcc gcaccccctg cccatacgct tgtagaaaaa
    22741 acaaacatca gaaaatacaa tatttttttc atgttcaact tccgtctgaa tgccgtctga
    22801 agccgcattc cgacatcaga cggcattgcc cgcacctgtg gataacttaa gcacggatgc
    22861 gtttcaacac ttcctctttg ctgattaatg ccaacacggc atcgacgctc ggggtttttg
    22921 ccgtaccgca gacagcaagg cgcaggggca taccgagttt gcccattttg atgccttctt
    22981 cgtcgcagaa aggtttgaag aggtcgtgaa tggctttggc gttccagtct tccagccctt
    23041 cgaggcgttc ggcgaagcgc agcatacggg cggcggcttc gtcgtcccag tgtttggcaa
    23101 catcggcttc ggcagggact tgttttttgt agaaatacag gcattcgtct gccaaggcgt
    23161 tcaaatcttg ggcgcggtct ttgaccagtg ccaacacgtc ttccaacgca ggtttttcgg
    23221 tttcatgaat atcgcgcagc gcaaggcgcg gtttgacgag ttcggtaagt ttgccgtcgg
    23281 gtgtgatttt gatgtgttcg ccgttgatcc aatagagctt tttcaaatcc atacggcttg
    23341 gggacgggga aacgtctttc aaatcaaacc attcgatgaa ttgttccgtc gtgaaaaact
    23401 catcgtcgcc gtgcgcccag cccaaacgcg ccaaatagtt gagcatcgct tcgggcagaa
    23461 tgcccattgc gccgaaatcg gtaatggcga cggtatcgcc gctgcgtttg gagatttttt
    23521 tgccttgttc gttgagaatc atcggcagat gcccgtattc gggcaggttc gcgccgatgg
    23581 ctttcaagat gttgatttgt ttcggggtgt tgttcacatg gtcgtccccg cggataatgt
    23641 gggtaacgcc catgtcgtag tcgtctacca cggcgcagaa gttgtaggtc ggcgtaccgt
    23701 cggcgcgggc aataatcagg tcgtctaggg cttcgttggg gatggagatt tcgcctttga
    23761 ccaagtctgt ccatttggta acgccgtcca aaggcgtttt gaagcggacg acgggttgca
    23821 cgcctgcagg aatttcgggc agggttttgc ctgcttccgg ccgccagcgg cggtcgtaag
    23881 tcgccgtgcc ttctttttcg gctttctcgc gcatcgcttc caattcttct ttgctgcaat
    23941 agcagtagta ggcatcgcct tttgccaaga gttcggcaat gacttcttta taacggtcga
    24001 aacggcgggt ttggtacacc acgttgtcgg cgttgtcgta atcgagaccg acccatttca
    24061 tgccgtcaag gatgatgttg acggattcgg cggtggaacg tgccaagtcg gtgtcttcga
    24121 tacgcaataa aaattcgcct ttatgatggc gggcaaacgc ccacgaaaac aaggcggtgc
    24181 gaacgccgcc gatgtgcagg taaccggtgg ggctgggggc gaaacgggtt ttgacggtca
    24241 taatggctcc gaaatctttg aaagcgttta ttttactgtt tttaccgtgc ttaggcatca
    24301 aaaatgccgt ccgaaccctg cctgcggata agtttcagac ggcattttct accgcttcaa
    24361 tcagacagcc atcccaaaaa gcggctgccc catttttcca aacggtagag ggataacgca
    24421 tacccctctt gcagcataaa gatttttttc ttatttcccg catcaaaccg cgtggtcggc
    24481 gtggcagaca tataaacgcg gacacccaaa tcctccgcca tttccgccgc ccgcgccaaa
    24541 tggtagggat cgctgacaat caccacgctg gcaataccgt tggcacgcaa aaccggacgg
    24601 atgttgttca ggttttcata agtgttgcgc gaagtgtttt caaacaggat gttgcgcgcc
    24661 ggaaccccct gtttgagcgc gtaccgccgc ccgacctcgg cttcggtcat atagcctttt
    24721 ttggttcgcc ctcccgtaaa cacgattttg cctaccctgc ggctttggta aagcgcgatg
    24781 gcgtggttga tgcgttcccg aaaaaccggg gaagggcgtt tgtcccacgc ggctgcgccc
    24841 aacaccagcg cggcatccgc ccggacatac ggcggcaaaa cctgcccgcc cgtccgataa
    24901 accgcccaaa cggatgaggc aaacaccagc aaaagcggaa aaacactcaa acagaaaccg
    24961 cccaacaggt aatagcgcaa gccgttgcgg ctgcaaaaca gccgtttgtt cacaataccg
    25021 cttcgatatt ttccaacggt ctgccgacag ccgccttacc gtttgccaaa acaatcggac
    25081 gctccaacag ggcgggatga tcggcgatgg cacgcagcag cgcgtcattg tccaaatcgg
    25141 ggttgtccaa acccaattcc ttgtacaaat catctttcac gcgcatcatc ccgcgcgccg
    25201 attccaagcc caatttgttg aaaatatcct tcaattcgga caagtcgggc ggcgtatcca
    25261 aatatttgac cgcttcggca gcaatgccgc gttcttccaa tagggacacg gcggcacgcg
    25321 atttgctgca acgcggattg tggaaaattt tgatttcaga catgacattt ccctacttct
    25381 cgacaatccc cttattatcg gcttacgcag ggttttactc aataccccgc ctccaaccgt
    25441 accaaacggt ttacaatacc cgaatcgaca tacaaaggac aaaacgatga aacgcttgaa
    25501 tctcgccgca atcgcccttg ccgccacatt tgccgcacat accgcctcgg cagacgaact
    25561 ggccggatgg aaagacaata ccccgcaaaa cctgcaatcg ctcaaagccc ccgtccgcat
    25621 cgccaaccta tgggcgactt ggtgcggtcc gtgtcgaaaa gagatgcccg ccatgtccaa
    25681 atggtacaaa gcgcagaaaa aaggcagcgt cgatatggtc ggcatcgcgc tcgacacatc
    25741 cgacaatatc ggcaactttc tcaagcagac cccggtcagc tacccgattt ggcgttacac
    25801 cggggcgaac agccgaaact ttatgaaatc ctacggaaac aatgtcggcg tactgccctt
    25861 taccgtcgtc gaagccccga aatgcggata caggcagacc attacaggag aggtgaacga
    25921 gaaaagcctg accgaagccg tcaaactcgc ccattcaaaa tgccgttaaa cgccggatgc
    25981 cgtctgaagc cgcttcagac ggcattttcc cgcccggcct tcggtatccg ccaaacttat
    26041 ccactatcta aaaacaggcg gaatctttat aatcggtact gtcttaccta ttgttcagac
    26101 ggcatatccc tgcggacgca accgcccgaa acgatatgcc gcccttcctt acaggacctc
    26161 ctatgatccg tttcgaacaa gtttccaaaa cctatcccgg cggttttgaa gccctgaaaa
    26221 acgtcagctt ccaaatcaac aaaggcgaga tgatttttat cgcgggacac tccggttcgg
    26281 gcaaatccac cgtcctcaag ctgatttcgg gcatcaccaa gccgagcatg ggcaaagtcc
    26341 tgtttaacgg gcaggacctc ggcacattgt ccgacaacca aatcggcttt atgcgccaac
    26401 acatcggcat cgtgttccaa gaccacaaaa tcctcaacga ccgcaatgtc ctgcaaaacg
    26461 tcatcctgcc cctgcggatt atcggctatc cgccgcgcaa agccgaagaa cgcgcccgca
    26521 tcgccatcga aaaagtcggc ttgaaaggac gagaattgga cgatcccgta accctctcgg
    26581 gcggtgaaca acaacgcctg tgcatcgccc gcgccgtcgt ccaccagccc ggcctgctga
    26641 ttgccgacga accctccgcc aacctcgacc gtgcctacgc gctcgatatt atggaattgt
    26701 tcaaaacctt ccacgaagcg ggaaccaccg tcatcgtcgc cgcacatgac gaaaccctga
    26761 tggcggacta cggacaccgc atcctgcgcc tctcgaaagg acgactcgca tgagcatcat
    26821 ccactacttc tcgctgcacg tcgaatccgc gcgctccgca ctcaaacagc ttctgcgcca
    26881 acccttcggc acactgctta ccctcataat gcttgccgtc gcgatgaccc tgccgctgtt
    26941 tatgtatctg ggcatccaaa gcgggcaaag cgtgttgggc aaactcaacg agtcgccgca
    27001 aatcaccgtc tatatggaaa ccgccgccgc acaaagcgac agcgataccg tacgcagcct
    27061 gctgacgcgc gataaacggc tcgacaacat ccgcttcatc ggcaaggaag acggtttggc
    27121 ggaattacag tccaacctcg accaaaatct gatttccatg cttgacggca accccctgcc
    27181 ggatgtcttt atcgttaccc ccgacccggc aaccacgccc gcccaaatgc aggcaatcta
    27241 ccgggacatt accaagctgc ctatggtcga atccgcgtct atggataccg aatgggtgca
    27301 aacgctgtac caaatcaacg agttcatccg caaaatctta tggtttcttt ccctgacgct
    27361 gggtatggct ttcgtccttg tcgcacacaa caccatccga ctgcaaatcc tcagccgcaa
    27421 agaagaaatc gaaatcacca aactcctggg tgcgcccgcg tcgtttatcc gccgcccctt
    27481 cctttatcag gctatgtggc agagtatctt ctccgccgcc gtcagcttag ggctttgcgg
    27541 ttggctgctc tctgccgtgc accccttggt cgatgccatc ttcaaaccct acggcttgaa
    27601 catcggctgg cgtttcttct acgtcggcga actcgggctg gtgttcggct tcgtcatcgc
    27661 gttgggcgta ttcggcgcgt ggcttgccac cacacaacac ctgctctgct tcaaagccaa
    27721 aaaataaaac accgtcaaaa attccgtctg aagccgtttt cagacggaat ttcgatttgc
    27781 cagtataatg gcgcatttcc ccaataagga ctccccacta tgctgacacc cgaacaagtc
    27841 aaggccctga ttgcaggcgt ggcaaaatgc gaacacatcg aagtagaagg cgacggacac
    27901 cattttttcg ccgtcatcgt ttcatcagaa tttgaaggca aggcacgcct cgcgcgccac
    27961 cgcctgatta aagacggact caaagcccaa ctggaaagca acgaactgca cgcactttcc
    28021 atttcggttg ccgccactcc ggcggaatgg gctgccaaag cacaataacc gccacacaaa
    28081 aatgccgtct gaaaccattt cgtttcagac ggcatttttt tatatcaaac cgtttactcg
    28141 ccgcgttttt tccaaagcgg ctacggcagg cagctctttg ccttccaaga actcaaggaa
    28201 cgcgccgccg ccggtggaga tgtagccgat ttgttcggta acgccgaatt tggcaatcgc
    28261 cgccagcgtg tcgccgccgc ccgcaatcga gaacgctttg ctttgggcga tggcttcggc
    28321 aagggctttt gtgccgcctg cgaattgatc gaactcgaac acgccgaccg gcccgttcca
    28381 aacgaccgta ccggcggctt taagcaaatc ggcaagcgcg gcggcggatt tcgggccgat
    28441 gtccaaaatc atctcgtctt cggcaacgtc ggcgatgtct tttaccaccg cttcggcatc
    28501 ggcggcaaag gctttggcaa cgacgacatc ggtcggcagc ggcacagaac cgcctttggc
    28561 cgccattttc gccataattt ttttggattc ttccaccaaa tcatgttccg ccagcgattt
    28621 accgatggct ttgccttccg ccaacaggaa tgtgttggcg atgccgccgc cgacgataag
    28681 ctgatcgact ttgtccgcca acgattcgag gatggtcagc ttggtggaca ctttgctgcc
    28741 ggcaacaatc gccaccatcg ggcgcgcggg ctgtttcaag gctttgccca aagcgtcgag
    28801 ttcgcccgcc atcaatacgc cggcgcaggc gacgggcgcg gcttgggcaa cggcttcggt
    28861 cgaagcttgg gcgcggtgtg cggtgccgaa cgcatcattg acgaacacgt cgcacaaggc
    28921 ggcgtaggct ttgcccagtt ctaaatcgtt tttcttctcg cctttgttga tacgcacgtt
    28981 ttgcaacatc acgacatcgc ccgcgttcaa agccggtttg ttttcgcgcc agtcgttcaa
    29041 tactttcacg tctttgccca acaggccgcc caagtgcgcg gcaacgggcg caacgtcgtc
    29101 ttcggggtgg aactcgcctt cggtcgggcg gcccaagtgg gtcatcacaa taacggacgc
    29161 gccgttgtcc aagcagtatt tgacggacgc gagcgaggcg cggatacggg tgtcgtcgct
    29221 gattttgccg cctttgaacg gcacgttcat atcggcgcgg atgaggacgg ttttgccccg
    29281 cacgttttgt tcggtcagtt ttaaaaatgc cataatcagt ccttttcaat cagtgtttgc
    29341 gatacagaaa caattgatgc cgcctgaagc cttcagacgg catcgcaacc cgatcagccg
    29401 gatacgcgct cgatgttcgc accgacgctg ccgagttttt tttcaatatt ttcataaccg
    29461 cgatccaagt ggtaaatccg ttcgaccacg gtttcgccgc gcgccgccaa accggcgata
    29521 acgaggctgg cggacgcgcg caaatccgtc gccttgacga ccgcgccgga aagccgttcc
    29581 acaccctgca caaatgccgt attgccctcg gttgtaatgt tcgcccccat ccggttcaac
    29641 tccggaacgt gcataaagcg gttttcaaaa atcgtttcca ccacgcggca gcttccttcc
    29701 gccacagcat tcaatgccat aaactgcgcc tgcatatccg tggggaagcc gggatggacg
    29761 accgtgcgga tgtccaccgc cttcggacgc tgccgcatat cgatggcgat ccaatcgtcg
    29821 cccgcttcaa tcaccgcacc tgcctcgacc agtttgtcca acaccacttc catcgttttc
    29881 ggggcggcat tccgcaaaac caccctgccg ccggttatcg ccaccgcaca caggaacgtc
    29941 cccgcttcaa tccggtcggg gacgacgctg tgttcgcagc cgtacagctc gcccgcacct
    30001 tccacaatca tggtcgatgt tccgatgccg ctgattttcg cgcccatttt gaccaggcat
    30061 tccgccaaat cgaccacttc aggctcgatg gcgcagtttt ccaaaaccgt cgtaccttcc
    30121 gccagcgtcg ccgccatcag caggttttcc gtaccgccga cggtaacgac atccatcgcc
    30181 acgcgcgcac ctttgagttt gcctttggct ttgacgtaac cgtgttcgat ggcgatttcc
    30241 gcccccatcg cttccaagcc tttcaaatgc tgattgacgg ggcgcgaacc gatggcgcag
    30301 ccgcccggca ggctgacttg cgcctcgccg aaacgcgcca gcgtcgggcc aagcaccaaa
    30361 atcgaagcgc gcatcgtccg aaccaattcg taaggggcgc aggtattgtt gaccgtaccg
    30421 ccgttgattt caaattcgct gatattgtcg gtcagaacgc gcgcgcccat gccctgaagc
    30481 agcttttgcg tggttgcgac atctgccagc atagggacgt ttttcaggcg caacgtaccc
    30541 gacgtcagca aacccgcgca catcagcggc aatgccgcgt ttttcgcgcc cgagaccgtt
    30601 atttccccgt tgagcgggcc gtttgcggag attttcagtt tgtccacgtt tgttccctcc
    30661 tggtgggtac ttgctaatat ttcaatactc gggacaatgc ataaagcatc acccgatgaa
    30721 ggttgcagag gcggaattat aagggatttt cgggaaaaat acggaagccg caccaaagaa
    30781 tttgacgaaa tgccgcgctt tccgaacaag gattgtcgga aaacaaaaaa aagccgagtt
    30841 ttgaaaactc agcttttttg ctttacctgg tgggtcgtga gcgattcgaa cgctcgacca
    30901 acggattaaa agtccgctgc tctaccgact gagctaacga cccgaaaaat ttgaaagttt
    30961 atacatcaag cctgatgctg tcaaccattt tgtcggcgtt cagacggcgt tttatttatc
    31021 aggctgtttt ttcgtataaa ttaacgaggt cagtatcgat gcacccaacg cgccgaacac
    31081 gaccgacagc gaaacggaaa tcgggatgtg tacccaatgc atcaccagca ttttcacacc
    31141 gataaaaccc aacacgaatg ccaagccgta tttcaggaag ataaagcgtt ccgccacatc
    31201 cgccagcagg aaatacatcg cccgcaaacc caaaatagcg aaaatattgg aagtcagcac
    31261 gataaacgga tcggtggtaa cggcaaagac ggcggggata ctgtccacgg caaaaacgac
    31321 atcgctcaat tcaatcataa tcagcaccaa aaacagcggc gtggcgattt ttttgccatt
    31381 ctcgacggta aaaaatttct cgccgtgaaa cgccgtgccg accggaacga ctttcttgac
    31441 ggcattcagc agcctgctgt ttgccaaatc ctcttcctca tctccttcgg gcttcatcat
    31501 gtgtatgccg gtatagagca ggaacgcgcc gaacagatac agaatccact cgaactgccg
    31561 aaccagtgcc gcgccgacga aaatcatgac ggtgcgcaat accaatgcgc ccaatacgcc
    31621 gtacagcagc acgcggtgtt gaaactgcgg tgcgactttg aagtagccga atatcatcag
    31681 gaacacgaaa atattgtcga ctgccaacga tttttccaaa atgtagccgg taaagaattc
    31741 caatactttt tcttttgcgg ctgccgcgcc gtagccggga ttgccggcga gttcgaaata
    31801 cagccagcct gcgaacaggc aggatacggc aacccacaag ccgctccatg ccaaggtttc
    31861 tttgatgccg actttatggc tgccgttttt cttcagcgaa aacatatcca aggcaatcat
    31921 gaccagcact gccgcaaaaa aaacgccgta aaacaacggc gacccgatgc cgggatattc
    31981 tgtcatggtt caatctcctg atttgaaatg tagttgtgtt accagctgat ataaaacatc
    32041 gcttttgcca aaaagacaat cagcagcata tgggtaaaga cgacggcgtg tatgtatttc
    32101 gaccagccga ccgtcagcgt ggaacgcgcc attttgacga cggcgatggc gaagtgcgcc
    32161 aacacgctga acgccaacag gattttcagc gtcagcatcg taccgaagga agtggcaaac
    32221 ggttcgcccg atataggaag gtagcggttt gccgccatca cgatgccgct ggcgaacagc
    32281 agtccgaccg caaacggcat caccctgacg gcgcggtaag acattgcctt ttccacttcg
    32341 cgccgcgcct cgcgcgacac ccgtcccgta tgcaggacgg acaaaaccag cacttcaaaa
    32401 aacacgccgc cgacaaaggc ggtggcgcaa tacaggtgga tgatgtgcgc gacggcataa
    32461 atactcatac gatgctccaa ccggaaaact cggatacgga ttgtatcact atcgcccccg
    32521 atgtccgcat accgcttccc gcaccgcctc ggcgattctc gcgcccgctc cgcgatgttg
    32581 tgcgataaag ccgtccacgc gcgcctgcat ctgcattccg cccccctcgc cagataagtt
    32641 tttttcaacg gcttcacgcc acgcgtccgc cgattcgact tgaaccgccg cacccgatgc
    32701 caaggcgtgc cggcaggctt cggaaaaatt gtaggtcgaa aagccgaata tcgtcgtaac
    32761 gccgcaggaa agcggttcga tgatgttctg acagcccgaa ccgaccagac tgccgccgac
    32821 aaaagcgaca tcggcgcaca ggtaatacgc atacagctcg cccatactgt cgcctatcca
    32881 cacctgcgta tcgggttcga ccggcaaacc gtcgctgcgc cgctgaacct taaacccgaa
    32941 gcgtttcgcc gtttcaaatg tcgtctgaaa atgctcggga tggcgcggca cgacggccag
    33001 cagggcatcg ccgcgatatt gttgccacgc cgccagcagt ttttccgcct cgtcttcgcc
    33061 ccgataaacg cgcgtgctgc cgcacacggc aaccggccgg cctccgatgc gtttttcaaa
    33121 ctgccccgcc agcgttttca tgtcttcaga cggcatcagg tcgtatttgg tattgccgca
    33181 cacctgcacg gatgccgcgc ccaatttcgc caaccgcgca gcatccgcct ccgtctgcgc
    33241 cagacagccc gtcagcgaag cggctgcggg acggatcagg cggcggactt tcagataacc
    33301 gttcagcgat ttttccgaca gccgcgcatt cgccaaaaac agcggcacac ccgctcgccg
    33361 gcattccttc atcagattgg gccagatttc ggtttccatc aaaatgccga acatcgggcg
    33421 gtgttcgcgc aaaaactgcc gtacccacgt ttttttgtca tacggaagat agcggcattg
    33481 cgcatcggga aacagaactt gcgcggtttc ccgtcccgtc ggggtcatct gcgtcatcag
    33541 cagcggcgca tcgggaaaac gccgccgcaa ctcgcgtatc aagggctggg cggcacgcgt
    33601 ttctccgacc gaaacggcgt gtatccaaac cgcgccggta acgggattcg gatgcggctt
    33661 gccgaaacgc tcgtccctat gcgcccggta tgccggggca cttccggagc gtttgtccaa
    33721 ataacgccgt atccatatcg gcgcaagcag ccacaataca tcataaagcc attggaacat
    33781 ctttctattt cctgcaaaac aaatgccgtc cgaacggttc ggacggcatt tcggcaacgg
    33841 aatcaaatat cgtaggttgt cgaagcggta tctccgccct tgcccgtcca gttggtatgg
    33901 aaaaattcgc cgcgcggttt gtcggtgcgc tcgtaagtgt gcgcgccgaa gtagtcgcgc
    33961 tgtgcctgca agaggttggc aggcaggcgt tcggtcgtat agccgtccaa gaacgtaatc
    34021 gccgaagcca tgcagggcat agggatgccg cattcgaccg ccttggcaac caccttgcgc
    34081 catgccggca ggcagttttc caaaatattt ttgaaatacg gatccgcacc caagaacacc
    34141 aaatcgggat tggcctcata cgcgtcgcgg atattgccca aaaacgcgct gcggatgatg
    34201 cagccttcgc gccacagcag cgcagtgttg ccgtagtcca aatcccagcc gtaactttcg
    34261 cccgcttcgc ggatcaacat aaagccttgt gcgtaggaaa tgattttgga ggcaagcaaa
    34321 gcctgtctca acgcctcgac ccattcttgt ttgccgcctt cgacgggcgt ggcggttcgg
    34381 gcgaacagct tgccggtctg cacgcgctgt tctttgaacg acgaaacaca gcgggcgaat
    34441 acggcttcgg aaatcagcgt cagcggaatg cccaaatcca aggcattgat gcccgtccat
    34501 ttgcccgtgc ctttttgccc tgccgtatcg aggattttct cggccagcgg ttcgccgcct
    34561 tcgtccttat agcccaaaat tgccgctgtg atttcaatca gataagaatc cagctcggtt
    34621 ttgttccact cggcaaacac gcggtgcatt tcgtcgtagg acagccccaa accgtctttc
    34681 ataaactggt acgcttcgca aatcagctgc atatcgccgt attcgatgcc gttgtgcacc
    34741 attttgacaa aatgtcccgc gccatccttg ccgacccagt cgcaacacgg ttcgccctgc
    34801 ggcgttttgg cggcaatcgc ctggaaaatc ggcttgaccg cttcccaagc gcgcttatcc
    34861 ccgcccggca tgatggacgg cccgcgccgc gcgccttctt cgccgccgga tacccccgcg
    34921 ccgacaaaca aaatcccttt tccggcaagg taatgtgtcc gccgtgtcgt gtcggggtaa
    34981 ttggcattgc cgccgtcgat aatgatgtcg ccttcttcca acagcggaag cagttgttcg
    35041 acaaagtcgt caaccaccga acctgcgcga accatcatca tgatttttct cggttttgcc
    35101 agcttatcga ccaagtcttg cagggaatac gcgccgataa tgcccgtctc ttttgccgca
    35161 ccgtttaaaa attcgtccac tttaccgatt gtccggttgt aggcgacaac cttaaatccg
    35221 caatcgttca tattcaaaat caggttttga cccataaccg ccaaaccgat tacgccaata
    35281 tcgcctttca ttgcaggaag ctccgttata gatttaattt atcggctgca actctacctg
    35341 atttacactt gtttaacaat ccttaacttt ttaatttttt gaaaagatgc ctttacgctt
    35401 tgccgtgccg tttccctgaa gggttataaa taaaatataa ggtttaaata ataaaacgat
    35461 gattatattg atatggggaa ttttctgtgg gtaacttttt tttattttta aaatcatctg
    35521 aattgccgtt ttaaaagggt gtcggtaagg aggattccct tttgtgcata cctgtggatt
    35581 gtttttcgtg gggaataggt tttgtggata gtttgcttgt tgtgcggacg acatcccgct
    35641 tttctttacc gaatggctgc cggtgccttt aagaacggga atgctgtgga tatttaagcc
    35701 ggaaatgtgt ttggaacttg tggggatgaa aaggtgtcgg cgcgaatgtc tttttttctg
    35761 catattggca gagtgtgtat ccgagtttgt gcataagtgg tggagaaaat aagatttttg
    35821 agtaaatctt atgatatttc aataagatgg cttttggctg gttgtgtata agcatagcgg
    35881 attaacgaaa atcaggacaa ggcggcgggc cgcaggcagt acaaatggta cggaaccgat
//
LOCUS       DAXDZG010000014        35196 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXDZG010000014 DAXDZG010000000
VERSION     DAXDZG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35196
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.014"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            109..2136
                     /locus_tag="ACFB78_000598"
     CDS             109..2136
                     /locus_tag="ACFB78_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC1268651.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGITTTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2226..3779)
                     /locus_tag="ACFB78_000599"
     CDS             complement(2226..3779)
                     /locus_tag="ACFB78_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC1268652.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIHKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFEGNK"
     gene            3830..3958
                     /locus_tag="ACFB78_000600"
     CDS             3830..3958
                     /locus_tag="ACFB78_000600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268653.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4030..5223)
                     /locus_tag="ACFB78_000601"
     CDS             complement(4030..5223)
                     /locus_tag="ACFB78_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC1268654.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGEAFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5220..6203)
                     /gene="patB"
                     /locus_tag="ACFB78_000602"
     CDS             complement(5220..6203)
                     /gene="patB"
                     /locus_tag="ACFB78_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC1268655.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6214..7650)
                     /locus_tag="ACFB78_000603"
     CDS             complement(6214..7650)
                     /locus_tag="ACFB78_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC1268656.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7780..8939)
                     /locus_tag="ACFB78_000604"
                     /pseudo
     CDS             complement(7780..8939)
                     /locus_tag="ACFB78_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9380..9592
                     /locus_tag="ACFB78_000605"
     CDS             9380..9592
                     /locus_tag="ACFB78_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC1268657.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9571..9732)
                     /locus_tag="ACFB78_000606"
     CDS             complement(9571..9732)
                     /locus_tag="ACFB78_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268658.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9740..11318
                     /locus_tag="ACFB78_000607"
                     /pseudo
     CDS             9740..11318
                     /locus_tag="ACFB78_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11400..12497
                     /locus_tag="ACFB78_000608"
     CDS             11400..12497
                     /locus_tag="ACFB78_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC1268659.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12611..13726
                     /locus_tag="ACFB78_000609"
     CDS             12611..13726
                     /locus_tag="ACFB78_000609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC1268660.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTAGNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNPASVSIENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVPPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSFAEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            13794..14306
                     /locus_tag="ACFB78_000610"
     CDS             13794..14306
                     /locus_tag="ACFB78_000610"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC1268661.1"
                     /translation="MPSERVSASDGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14383..14859
                     /locus_tag="ACFB78_000611"
     CDS             14383..14859
                     /locus_tag="ACFB78_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC1268662.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14847..15773
                     /locus_tag="ACFB78_000612"
     CDS             14847..15773
                     /locus_tag="ACFB78_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC1268663.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15838..16395
                     /locus_tag="ACFB78_000613"
     CDS             15838..16395
                     /locus_tag="ACFB78_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1268664.1"
                     /translation="MKPKFKTVLTALLLAVCLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATLGLYQNVPVQPVKIRRVVVGQ"
     gene            16482..18539
                     /locus_tag="ACFB78_000614"
     CDS             16482..18539
                     /locus_tag="ACFB78_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1268665.1"
                     /translation="MDKFKPRILKCCQPNPTQPNPSPDLSDTAGANTEAIYTSDGITA
                     NSTQLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNL
                     PPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGS
                     DGFVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIAREL
                     LKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWI
                     FVFAKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTG
                     KKYPANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDF
                     GKVAVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPK
                     PSQLIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAE
                     KSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFS
                     PEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLLNK
                     GFTSADLLAFIKKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVI
                     IRV"
     gene            18629..21568
                     /locus_tag="ACFB78_000615"
     CDS             18629..21568
                     /locus_tag="ACFB78_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC1268666.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(21662..22621)
                     /gene="ttcA"
                     /locus_tag="ACFB78_000616"
     CDS             complement(21662..22621)
                     /gene="ttcA"
                     /locus_tag="ACFB78_000616"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC1268667.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(22713..23576)
                     /locus_tag="ACFB78_000617"
     CDS             complement(22713..23576)
                     /locus_tag="ACFB78_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC1268668.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQAGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAE"
     gene            complement(23611..23973)
                     /locus_tag="ACFB78_000618"
     CDS             complement(23611..23973)
                     /locus_tag="ACFB78_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC1268669.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            24048..24527
                     /gene="ybaK"
                     /locus_tag="ACFB78_000619"
     CDS             24048..24527
                     /gene="ybaK"
                     /locus_tag="ACFB78_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC1268670.1"
                     /translation="MSKHTYPITPAVRVLHENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            24549..25241
                     /locus_tag="ACFB78_000620"
     CDS             24549..25241
                     /locus_tag="ACFB78_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC1268671.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLIIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            25245..26120
                     /locus_tag="ACFB78_000621"
     CDS             25245..26120
                     /locus_tag="ACFB78_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC1268672.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            26328..29756
                     /locus_tag="ACFB78_000622"
     CDS             26328..29756
                     /locus_tag="ACFB78_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC1268673.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            29971..30954
                     /locus_tag="ACFB78_000623"
     CDS             29971..30954
                     /locus_tag="ACFB78_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC1268674.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            31225..32715
                     /locus_tag="ACFB78_000624"
     CDS             31225..32715
                     /locus_tag="ACFB78_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1268675.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGNHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITLKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRRDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(32783..34180)
                     /gene="aspA"
                     /locus_tag="ACFB78_000625"
     CDS             complement(32783..34180)
                     /gene="aspA"
                     /locus_tag="ACFB78_000625"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC1268676.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(34256..34393)
                     /locus_tag="ACFB78_000626"
     CDS             complement(34256..34393)
                     /locus_tag="ACFB78_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268677.1"
                     /translation="MAEHNPPKDILAAGGACRKVRKVTTENNVSRFQTAYDMDNFIRP
                     V"
     gene            complement(34459..35019)
                     /locus_tag="ACFB78_000627"
     CDS             complement(34459..35019)
                     /locus_tag="ACFB78_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC1268678.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT         8522 a         9020 c         9677 g         7977 t
ORIGIN      
        1 ggccggtttg tgcatttcgt catccggaag ccggtatttt gccggcaggt tttgactact
       61 ttgcccgatg accgggtttg tctgttttta acaaaggagt gagaaggttt gtccctgtct
      121 gaatttatag aacgccgaac gtcatttaat ccgatggtta ttttgacgac tttgtttttt
      181 gtgtgtgttt tggtggtatt ggttttaacc gtgccggatc aggtgcagat gtggctcgac
      241 cgggcaaaag aagtcatttt taccgagttc agctggtttt atgttttaac gttttccatt
      301 tttctgggtt ttctgctgat actctcggtc agcggtttgg gaaacatcag gctaggacgg
      361 gatgaagatg tgccggaatt cggcttcctg tcgtggctgg cgatgctgtt tgcggccggg
      421 atgggcgtgg gcctgatgtt tttcggcgtg gcagagccgt tgatgcatta tttttcggac
      481 attacggtcg gcgcgccgga acacaggcag cagcaggcat tgctgcacac ggtgttccat
      541 tggggcgttc acgcctggtc ggtgtacggt acgattgcat tggctttggc ttatttcggt
      601 ttccgctaca aactgccgct tgccctgcgt tcttgttttt accccctgtt gaaagaaaaa
      661 atttccggaa ggttcggcga tgccattgat attatggcgt tgcttgctac ttttttcggc
      721 atcaccacca cattggggtt cggggcttcg caactgggcg ccggattgca ggaaatgggc
      781 tggattgccg aaaacagctt cggcgtgcag gtcttgatta tcgccgccgt aatgtccctc
      841 gccgtcgttt cggcaatatc cggcgtgggg aagggcgtga aggtgttgag cgagttgaac
      901 ctgggccttg cgtttttgct gctgtttttt gttttggcgg cgggccccac tgtttacctg
      961 ttgtcggcat tcggcgacaa catagggaac tacctcggaa atctggtgcg cctcagtttg
     1021 aaaacttatg cgtacgaacg ggaacacaag ccgtggtttg aatcttggac ggtgctttat
     1081 tgggcgtggt ggtgttcttg ggcgccgttt gtgggtttgt ttatcgcgcg catttcaaag
     1141 gggcgcacca tccgcgagtt tgtcttcggg gttttgctca tccccggcct gttcggcgtt
     1201 ttgtggttta ccgtcttcgg caatacggcg atttggctga atgacggggt tgcgggggga
     1261 atgctcgaaa agatgacctc ctctccggaa acgctgcttt ttaaattctt taattacctc
     1321 cccctgcccg aactgacgag catcgtcagc ctgctggtca tttccctgtt ttttgtaact
     1381 tctgccgact ccgggattta tgtcctgaac aatattacct ctcgggacaa aggcttgagc
     1441 gcgccacggt ggcaggcggt tatgtggggc gtgctgatgt ctgccgttgc cgttttgctg
     1501 atgcgctcgg gcggactcgg caacctgcag tctatgaccc tgattgtttc cctgccgttt
     1561 gccctgctga tgctgataat gtgtttcagc ctgtggaaag gcttgagtgc ggataagaaa
     1621 tattttgaga ccagggtcaa ccctaccagt gtattttgga cgggcggcaa gtggaaagaa
     1681 cggctggtgc ggataatgag ccagacgcag gagcaggata ttttaaaatt cctcaaacat
     1741 accgcatcgc ccgctatgca cgagttgcaa cgggagcttt cggaagaata cggcttgagc
     1801 gtccgggtcg ataagatgtt tcatcaggac gagcccgcaa tcgagttcgt cattcggaaa
     1861 gagacgatgc gcgattttat gtacgggatt aagtctgtcg ggcaggatgt atccgaccag
     1921 ttgattaacg acggcaagct gccgcatatc cggcaccaga caacttacaa accctacgct
     1981 tattttttcg acgggcgcgt cgggtacgat gtgcagtata tgaacaagga cgagctgatt
     2041 gccgacattt tgaaaaacta cgaacgttat ttgatgttgt tggatgatgt cggtcaggaa
     2101 ctgatggcgc acgagcaggt ggaattggca gagtaaatat cctcgctccg gcgtttgcca
     2161 aaaaaatgcg gtgaatcaga ttcaccgcat ttttttgccg gacggggctt cggacggcac
     2221 ggcgttcatt tgtttccttc aaactgctcc ttcagcaccc gtttcaatac tttgcccgta
     2281 gcgttgcgcg gcagcccgtc tttaaagtgg atctgtttgg ggattttgaa atttgccagc
     2341 acggtacgca ggtggcggcg gatttcgtcc tcgcccaaat ccataccttc cttcaattgg
     2401 acgaaggcga cgatttcctc gtcggcataa cggtctttca cgccgatgac ggcggcggct
     2461 tcgacggcat cgagtttgtg gatttcttct tcgatctcgc gcggatagac gttttgacct
     2521 ttggaaataa tcaaatcttt tttgcggtcg acgataaaga taaagccgtc ctcgtctatg
     2581 gtaacgaaat cgcccgtttt caaccagccg ttgacgatgg tttcatcggt ggcggcaggc
     2641 atattgaggt agccccgcat caccgaaccg cccctgacga tcagttcgcc cacttcgccg
     2701 cgcggcactt cgaccaattc ttcatcgacg gctttggctt ccaaaccggg cagggggatg
     2761 ccgacgctgc gggctttttg ccgttcgggc gtattgacgg cgacgacggg cgaggcttcg
     2821 ctcagtccgt agccttccag caatttggcg cgggggaact tggctttaaa atcgaggatg
     2881 gtttgttccg ccaaaggcgc gccgccgctg ataaacaggc ggatgcggtt gaaccatctg
     2941 aaataccaag ggatttttgc cttgctcatc gcggtgtaaa tcgcgggtac gcccaaaaac
     3001 acggttgcgc gtttgagcag ggcctgtttc aaaacgttgg aaaaggggaa aacggatttg
     3061 accaaaataa tcgaacacgc catataaatc ggcagcagca ccatagccgt cagcgtgaag
     3121 ctgtggaaca tcggcaggaa aacgataaag cggtcgcgtt tggaaatttt aaagatgcgt
     3181 tcgatgccgt tcaggttggc gaacaggttg gcgtaactga ttagcgcgcc tttgggatgc
     3241 cccgtcgtgc cggaggtgta gatgatgtgt gccaaatcat ttatccgggg ttggcggccc
     3301 aagtcgggtt tttcggggaa gcggcgcacg ttttcaaaaa aggcatcgcc ttccgccgtt
     3361 tcgccggccg gccggctttt gtccgtccaa atgatttttt cgacgggcgt ttgcgccttc
     3421 aggcccgcca attcttttga caggccggcc gaggcgaaca ggaagcgcgc cttgcagtcg
     3481 ttcaggatat acgcgtattc gctgtttttc aaaaatgtgt tcatcggtac ggcgaccgcg
     3541 ccgatggcgg agacggcgaa ataggcggta ataaattctg tggaattgga aaccgccagc
     3601 gcgaccgtgt cgccgaactt cacgccgata ttttgcagat acgccgcgac ggcttcggct
     3661 tcctgcttga gcgcgcggta ggcggttttt tccttgccgt cgaacactgc cgtgccgttg
     3721 ccgtttttgc ggcaggcggc ggcgagcatt tcgtagaaat tggcataagt ccggttcatg
     3781 aaacttcctt tatggttgcg gacggcgaac gccctcattt gaaaacaata tgccgtctga
     3841 aaacggaagg cggcagttta tcatctttgc cttcagacgg catatcaaac gccgcctttg
     3901 cgccgcatgc cggaggttcg cccccgtcct tcctatgtct gacctgaatg ccgtctgaat
     3961 caatatgaag atatacgttt cagacggcat ccccctgttg tggaaatacc gtccgccccc
     4021 cctgtccgat tattgcctga ttgcggcggc gcggacgagt tcttcgaggc tgtcggcaag
     4081 catttccgcc gcgcgccggt agccttgggc ggagaagtgt acgccgtctt tggcggccca
     4141 tccttggttg agccagtttt tcatgctgca tatgccgccc attgcgtttt gccaagacca
     4201 aaacatcgtc tgcccctgac gggcgacgcg ccgctgcatc tgttggactt cggtcaggag
     4261 gacggggcgc gtgccgcata cgccgagcgt gtttttcagg gattcgggcg cgccgatgat
     4321 gaggatgccg gcggcgggca ggctgtcgcg gatttggcgg acggtatcca gccatttttg
     4381 ttcggtatcg gcaatgtcga tgttgttgtt gaaggcttcg ttggtgccgt aggaaaggat
     4441 aaccaaatcg gcgccggttt gggcaaggtc gttcatacgg tcggcacgcc atttcgacca
     4501 ctgggtcaat tgtgcgccgt tgatgcccat cgcggaaacg gtaatgccgc cggcgggatt
     4561 ttcgatgttg atgaagccga tgtcccacgg catttcggtc tgtatggcca ggggcagtgc
     4621 cgcgcccgta tccagtacct gccagccgcc gccgttggcg gagacggtgt tgccgttgac
     4681 ggtcagggtt tgttcggcga gcagcggttt ggcaaacagg gaaacgcgct gtttgccggt
     4741 tttgccgtca gacgcggtca gggtcatgcc gccgccgctg ccggtttggg cgaggatgcc
     4801 gccgagcggg aaatctccgg tattgttcct gctggtgaag ctttgccagt tgccgctgtg
     4861 acggacggcc gccatgcgct gccctttgac gttggcgggg taaacccagc ctatgccgcc
     4921 gtcgccccat gttttttgca ggcgtttgcg cagggcgtcg gtaaagaagt cgccggcggt
     4981 atgcgagtcg ccgatttgca ggatgcggaa ggcctcgccg ctgccttgtg cgacggatcg
     5041 gagttttttc atccaaggcg aggcggcggc gttgccgtag tcggtcagca gcccgtttgt
     5101 atcggtgtag ggggcggacg gggaaacggt aacggtgtcg gggctgacgg aggcgacggg
     5161 cagggcttct gcctgcgtgg cggcgaacag ggcggaaaat gcgatgaagt gtttggggtt
     5221 catggctgtg tacttgatgg ttgcgtactc ggttcaaaaa cgattttttc cattattttt
     5281 tccgccagca gtttttgtcc ttcggcggta aagtgtatgc cgtccttact gcggtagcgg
     5341 acgggtttgc cgttgacgtt gacggaatcg gtgtagcggc ctttcccgcc gctcagtgtt
     5401 tgcgcggtgg gaatcaggat gattttgcct ttcaagtgtt ccgaaagcag tttgtcgagg
     5461 tagcgcatct gaccgtcgag cttgactttt ttcatgtagg ggatgccgag ccagacgact
     5521 tggacgcggt gcgtgtgtgc ggcttcaagg atgcggtcga cgcgtttcag gtattcttgc
     5581 gcccattcgt cggaagcgaa tttgaggtag cgtttgccga cggggaaatc ccacgggtcg
     5641 ttcgggccga ggaagacggc gagtacgctg atttcgggat gttttttcaa ggtttcttca
     5701 atcgttttcg gccagtcgaa gaatgaggga taggaaagcc ccgtgctttg tttgctgagg
     5761 ttggcggatt cgatgccgta ttgctgtttc aggctttttt gcacgaaggg cgcaacgccc
     5821 tgcatcagcg aatctccggc gaaaaagact ttgtcgccgc tgcggacggc ggcggcttcg
     5881 gtgccttgtt tccattctgt tccgccggcc ggcggggcgg cttcggatgc ggcggcttca
     5941 ggcggcatat ctgccgaacc gccgtcttga gccgtcggcg gcgtttcgcc ggacaggaag
     6001 gttttgatgc cgtctgaaag ggcgtaggcg ttttcttgca acgccgctcc gctccgccac
     6061 catccgtagg cggcaagcgg ttcgagcggg ctgttgcggt ggtaggtctg ctgccagtag
     6121 gcgttgatgg ggttttggct gaaccacacg gcaatcaggg cagacatcag tatggaggcg
     6181 aaaagggaaa gaaagttttt catgtccaaa cccttaaaaa ttggcataaa taaagccggg
     6241 tatccccgaa ggggcgagga cgataatcag cagcaggatg atggaaatct ggataaacca
     6301 cagccacatc gggatttttt ccaaaccttt gaccgtgccg tcgaaagcgc gttgcaggta
     6361 agggtagagc agcatcaagg atgcaaacga ggcaagcagc agcatatccg cccgttgcgg
     6421 cgcgttccag ccgccggcat tggcaaagag ggcactgaaa actgcgcctg catcgtcggg
     6481 gtttgcggta ttgaagacga caaagctaag gcagacgaaa tggaaggtaa caaaccacga
     6541 gagcggcgca aggtatttca gacggcatag cgcgtcgcgt ccgaaatacc ggtcgcccgt
     6601 gttgagcagc gccagtgccg tgccgtgcag cgcgccccaa atgaggaagt tccagccgta
     6661 gccgtgccag atgcctgaga gtaccattgc cgccatcagg ttgagctgtg tccgcaaaaa
     6721 gccttttttg ctgccgccca aggggatgta gatgtagtcg cgtatccagg tggaaaggct
     6781 gatgtgccat ttgtcccaaa atgcgcggat attcgcggca cgaagcggtg cggagaagtt
     6841 tttgggcagc cggaagccca gcagcatcgc cataccgata accaaatcgg aatatccgga
     6901 aaagtctaaa aagagttgga aggtatagcc gtacacgcct gccaacacgc cccagccgtc
     6961 gaattgggcg ggattttcaa atacgggcga cacccagttt tccgccagca tccccgccag
     7021 ccaccatttt ttggcaatgc ccagtaaaat cagggaaacg gcgagggcgg ggcggacggg
     7081 cgaacgcggt cggcgggtgc ggatttgcgc cagtgcaccc gcctgttcgc cgtccgtgct
     7141 tttgaatgcg gcggcgcgga taatcgggcc ggaggtaacg gtggggaaaa aactcaggtg
     7201 cagcagcagc tcgtgccacc caaaacgcgc ggcgtgcggg gcgcggaagc agtaaaccag
     7261 ataggcgaca gactggaagg tataatagga aagccccagc ggcatcagga tgtcgattgc
     7321 gccgcctttt ccggcatatt gtgcaatcag cggacggaaa aaatcgaaat atttgaaaaa
     7381 gcccaaaacg gtaatcgagg cggcaatgcc gcaccccagc caaaagcggc gcgtactttc
     7441 gcgatcggaa cggagcagtt cgcccaaaag gtacacgcag gaggaataaa ggacgatgat
     7501 tgccgcaaat acggggctaa tatggtagag ccagcccata ccggcagcca aaagcagcag
     7561 gttttggacg gacgggtatt tcgcaaagcc ccagtaaatc ggcaggaaga cgataaaaaa
     7621 cagtgcgaac tcgacagaca gcagcggcat aaaccatcct tgggacaaaa aaataggcgt
     7681 taaaacacgg caaagcccgt tattttaagc aggggcggat attccttcaa tcaaaatgcc
     7741 gtctgaaggg cgggaggggc ttcagacggc atcgctcggt caggttcgga tatggtggtt
     7801 caaattcagg cagacccgca aacctgccgc cgccgtcagc atacccgcaa cgccgatgca
     7861 ggaaatgccc gagtattgcg taacctaatg tcccagcagt gcgccgccgc ctataccgac
     7921 gttgtaaagc tccgaataaa tcgagttggc aaggtctgcg gcatcggagg cgaaatccaa
     7981 aactttggca accatcccca agctgacgac cacaatcgcc gtcccccata cgaacaccaa
     8041 cgcgtaaatg gcggcaggaa aatgcgccaa aggcagcaaa agccccgaag agagggcgat
     8101 aagcgacacc gcgcccgcca aaaatgcgcg cggatgtttg gcaaaccatt tgccgaacag
     8161 gtaagacgcg gcaaaacccg ccaggccgta cagccccgga accaccgtaa cctgccgcgc
     8221 ggagaaaccg ccgatttgga tgacgaaggg ctcgatgtag ctgtatgcgg taaagtgcgc
     8281 cgtaatcacc ggcaccgtta ccgcatacag cagcatcagt tttttccgtt tcaaaagcag
     8341 gggcaggctg cttaaagaac cggtattcac gctcggcagg cgcggcaggc ttttggcaag
     8401 cactgccatc accgccgccg aacacaggcc gatcagcaga aaactcgcct gccagcccaa
     8461 atattgtccg accatacgcc cgagcggaat ccccgccgcc atcgccatca ccgtccccgt
     8521 gctgagcagc cccaatgcct gattcccttt tccgacaggg gcgatgcgta ccgccagcga
     8581 agccgtaatg gaccagaaca ccgcgtgcgt cagcacaatc cccacgcggc ttgcgagtag
     8641 gatttcaaaa cgccacgagg caaacgacag gatgtggctg acagtaatac agcgcaaaca
     8701 ggaccagcag caggccgcgc cgttccataa ttgcgcgtca gcagcatcag cggcagcgaa
     8761 gtcagcgcga caatccacgc ataaaccgta atcatcacac ccgtttcggt cgccgccatg
     8821 ccgaagcttc gtccgatgtc gctcaaaagc gcgatgggga tgtattcggc cgtgttgaaa
     8881 ataaatgcgc cgattgccaa ggcaacgatg ctcaaccaag gtttgtgcga agcgttcatc
     8941 tcaattcctt atatgtcgca ccccgccaaa agggggcgca ttatggtgaa ttaacaaaaa
     9001 ccggtacggc gttgccccgc cttagctcaa agggaacgat tccctaaggt gctgaagcac
     9061 caggcgaacc ggttccgtac catttgtact gcctgcggcc cgccgccttg tcctgatttt
     9121 tgttaattca ctatactttt cttgtctgcc ggacggcaaa atccaaatca gggggaagca
     9181 gccgcctccc gttcggagga acactcggga cgctgctttg aaaatcgttc catatgttga
     9241 aacattgccg cctcccgatt gggcaaggtt gccattatgt cgcaagcatc cgtgccgcac
     9301 cgtttccgcg tttgcccaag ttcacgctcc gacttacaat aacgcccgtc agattgcagt
     9361 accaacgaaa ggaaaaatcg tggaaaccct cctcctcgac atcgtcggca tgagctgcgg
     9421 cggctgcgtc aaaagcgtta cccggatatt agaaagcgta aaaggcgtgg caagcgtcga
     9481 agtcagcctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgcagcgga
     9541 agcactaatc gaagccgtag aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
     9601 aaatgccgtc tgaagccttt gcgtttcccc gatgccgtct gaagcacgct ccgcgccttc
     9661 gggcggcatt tgatttagtt gaacgggcag gatgaattgg ttaaacttgc gggcaatagt
     9721 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgctgcaa
     9781 cggagaacgg tgtgccgggc gtgtccgaca tcaaaaattc atatgatgat ttgatgtacg
     9841 aatccaaagc attttcccaa accgccatca acgccttgga ggcatgcgcc cgcatgatgg
     9901 gtttgaagcc tacggcagcc gcaaaagcca aggtcttgga gctgggctgt tcgatgggca
     9961 gaaacatcat cacgcaggca ctttattacc cggatgcgga atttgtcggt atcgacttgt
    10021 ccggcaggca ggttgcgcag ggcaacgcca tcattgaaaa aatgggcttg gaaaatgtgc
    10081 gtctggaaga aaaagatatt ttgaccgtcg atgaatcatt cgggaagttc gactatatca
    10141 tcgtccacgg catttggtcg cgggtgcccg acgcagttaa agacaaaatc ttttcgattt
    10201 gccggaacaa cctgaccgaa tacggcattg cctatatttc atacaatgtt taccccggtt
    10261 ggaagcggca ggagcagttg cgcgatatta tgcagtttgc cggcagggat gcgcttggag
    10321 aacccttgga agcaaggacg cggaaaggct tggatgcgat caaggcgctg gcggaaattt
    10381 tggaaaacga caagggcttg ggcggcggca aacttccggc gattcaaaaa atattgaatc
    10441 acaatactta ttatgtcgca cacgaatata tggaaatatt caacaacccg atttacgtta
    10501 acggtttcat tgaatgggca aaccgccacc ggctggcgta tatcggggat accgatttgc
    10561 atgtgtcttt tgtttcttgg atggcggagc atacgcggga gcggattctg gcattggcag
    10621 ggggtgatta tattgccaaa gaattttaca gcgatatttt atccgaccgc caattccacc
    10681 gttcgctttt atgccgtgag gaagtcgggg atactgtcag gcgtgatgag tcggttgccg
    10741 tcgaagtgat agaaagtttg aatttccgcc cggcaagagg ggaaacaatc aactttgatg
    10801 aaaacgatac cctgctttcg ggcatacgcg atgtgatgaa aaccggagag gcgtttaaaa
    10861 cggaagatgt tgcggaaaat cttgcccgcc gatttcccgg tctggaattt gaccgcatga
    10921 aaatcaattc ccagctttta ttgcaaacca ttctcgggcg tttttctgtt tcatcagaca
    10981 atgcgggcaa accatttttt gaagaccata aaacctatgt gccggcgcgc tttacaaact
    11041 atgccgccgc ctttgtggaa cacggtgcgg gagcgtttgt ccggcctgcc aaccgttaca
    11101 acgaaagcac gccctcattc ggatacgggc atttgtacat tatgcggcaa ttgtcgcggc
    11161 cgacgagcaa acaggcattg attgaaacgg ttgccgaaaa cttgaacatc gtcagcgcca
    11221 cgcccgacgg tttgacattc catccgcctg ccgaagtgta tgtggaagaa atattggcag
    11281 acttggcaga caggcatttt ctcgtttcgg cggattgacg ggcaagtccc gacggcaatc
    11341 cgtccgatat attttcagac aggaaagaca cacactgcga aaaacggtac acgacacata
    11401 tgaaccagac atttaccttg cccgatacgc gcccgtatcc ccaaaatccg attaaaaacc
    11461 acctgctgct caatgcctac cagttggcgc acaattcgtc acaggcttcg cgcaaactct
    11521 cgtccgggca gcttcaaacc gaaatcaggg ggatgcttga gcaaaaccac tatatcaacc
    11581 tttccctcgc gctgacgatg tcgcccgatg ccggaactta tgccgcgctg ctttccagtg
    11641 tgaacgcggt actcgattgc gagaaagaag gcgaagtgca gtggttcgcc ctgccggtcg
    11701 tgctggtgtc aggctgcaag aaagaacggg caatcgagat gaagctgccg acggaggcat
    11761 tgtttgcctg cctgcaaaac tatccgcacc tgcgcgcgtt gacgcaagag acacaatggc
    11821 tgccttatct tgtgcattct tccgatttga gtgcggtcgc gccggatgag tggtggcgtg
    11881 ccaaacaaaa taccgaagcg gcggcgcaac acttacgccg tttcgccccg cgccctttgc
    11941 tgttgcccga agggcagtcc gtccacgtcg tttacgtgct gggtttcggc agcggcaaag
    12001 ttcaggcggc attggggcag aaccttcttc aggcaggctt gcccttaatg caggtatggc
    12061 aggaaaatct tgcatcggaa ggcattacgc tgtttgccaa tccgctttcc cccgataccc
    12121 cggcgcgcgc gctttcagac ggcagccaca cgcgccaacg tatggcgatg gatgtttttg
    12181 cggcaaacgc gatacgcgcc gtccggatgc agggtccgcg cgtcggcgtg gtcgctgcgg
    12241 caaaggcggg cggacagatt ctattcggat ttaatgcgac cgacggcgcg tttgaagtcg
    12301 tgccgcaggt gttttgctgg cagctttcct ttaccgataa cattgccgtc atccagcaga
    12361 atttcctcga cctgatggca gaatgccgtg tagaacacgt ttacctgctg cacaatccct
    12421 tgggcgaaca ggaaaacatc ccgagctatg cggaagcgtt gaaacgggaa gggcgcaatc
    12481 cgtttttcag cgcatagtga tttgtagtag tgataggctt tctcatttct ataataaatc
    12541 aaaataaaga gcacaacact ttttcattct gtgttgtgcc ttgagtgaaa cgaaaggatg
    12601 aattataaat atgaagattg taattgcacc ggattcgttt aaggaaagct tgacagctca
    12661 acaggtagct gaagcaataa aaagaggctt ccaacaatcg atagcagatg tggaatgtct
    12721 cctctgtcct gtcggtgatg ggggagaagg cactgtagat gctatccgac attctcttga
    12781 cctagaagaa aaatgtctcc aagtgacagg accttttgga caaaaagaag tcatgcgcta
    12841 ttttcaaaaa gaacaactag ccctattcga agttgctgat ttggttggtc ttggaaaaat
    12901 cccgctagag aaacgaaatc cattacaaat ccaaactcgt ggtattggag aattgattcg
    12961 ccacctcatt agtcaagaaa ttaaagaaat ctatattggc gttggcggta cggccggtaa
    13021 tgatggaggt attgggattg ctgctggttt aggttatcaa ttttatgatg aggatggaaa
    13081 tgccttaccc gcttgcggtc aatccttact aaacccagct tctgtttcaa tagaaaatcg
    13141 ctatgaaatt cctgaagatg ttcacattcg tattttagca gatgttgtga gtcccttatg
    13201 tggacaccaa ggtgcgacct atacgtttgg caagcaaaaa gggctggatt ctactatgtt
    13261 tgaggccgta gatcaggcaa tacaagattt ttatgaaaaa gtcccccctg caacattaaa
    13321 acttaaagga gcaggagctg gtggaggcat cgctggtggt ttgtgtgcct ttgctcaggc
    13381 aagtatcgta tctggaattg acacctgcct ggacttgatt gactttgata agaaagtgtc
    13441 agatgttgac ttggttatcg ttggtgaagg aagactagat cgtcaaagtt tagcagggaa
    13501 agcgcctatt ggtgtagcaa aaagaacccc tgtcggagtt cctgttgtcg ctatttgcgg
    13561 cagtttcgct gaagatttgc cttccctacc atttgaaaat atccaagctg ccttttctat
    13621 tttggagaaa agtgaacctt tagaagatag tttgaaaaat gccagtcttt atttggagca
    13681 tacggcttct aatattggac atttattaaa tatgcctaag atttagccaa gccatttctt
    13741 ccagatggat gttttggcag gttcttcctt atgcggttac gattaaccgg ccgatgccgt
    13801 ctgaaagggt ttccgcttca gacggcatcg cgttggaaca gggatgggat atgaaaaaac
    13861 cgaaaatcct ttttgtctgc ctcggcaaca tctgccgttc gccgatggcg gaatacattt
    13921 tgcgccgccg tgccgccgaa gcgggcattc ccctcgaaac ggacagcgca gggacatcgg
    13981 gctggcacga cggcgaggat atgcaccgtg aaacggcaaa gatattgaaa aaatacggca
    14041 ttgacgcttc aggctttacc agccgcaaaa tccgccaaag cgatgcgacg gcgtttgact
    14101 acatcatcgc tatggacggc aagaatttgt ccgaattgga aaaaactttt ggcaggcggc
    14161 cggaaaaaat attcaagctg accgacctga tacccgaaag cggttacgac catgtccccg
    14221 atccgtggta catgggtgat tttgaagaaa catacaggct tgcggatgca ggctgtcggg
    14281 cattattgga aaagatttcc aaataaagca attgaataca aacacaaata taaaaccgcc
    14341 tcctgtcgta tcggtcgttc ggacggcata aacagggaat ttatgaaaac aaatttcaaa
    14401 cagaaaatta tcgaacaggc acgcagagag gacttgcagg taaccgcttt gcgcgagcag
    14461 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaatgt tttgtcacag
    14521 atgcagcagc agagcgaagg cgtgcttgca ccgcctaccg cctaccgtgc ccttgatttt
    14581 tgggcggatc agggcgtttt acacaaagtg gcggcggtca acggctatat tttgtgcagc
    14641 cacgcgcagc acgagtgcaa cgaccattgc cacgaccacg aagaagccga agcgcaccac
    14701 agcgcgttta ttttggtctg caccgaatgc ggcgcggcgg acgagcaaac cctcagccac
    14761 gagtgggcgg cactgcgtgc aggcgttgcc gaaagcggtt ttgcgctgaa agaagaacac
    14821 gttgttttaa ctggaatctg taaaaaatgt cagaagtgaa aaaaaccaaa gtccacctga
    14881 tttcaggttt tctgggaaca ggcaaaacca ccgcgctcaa aagcctgatg gcgcaaaaag
    14941 acccgaacga aaaatgggtc atcatcgtca acgagttcgg cgaaatcggc attgacggcg
    15001 cggtattgag cgacaacggc atccctgtgg cagaaatcgc cggcggctgt ttatgctgca
    15061 ccgccggccc gcaaatgggc gtaaccgtgc agaaaatgct gcgcgacgcc aaacccgacc
    15121 gcctgatgat tgaggcaagc ggactggcgc acgccgccag cgtcatcgac gaattgaaag
    15181 ccaaacctct ggacagtctt ttggaaatcg gcgccgtctt taccgtcgtc gatccgcgcc
    15241 agttcatcaa tcccgattac gcgcaacagg cgttgtataa agaccaaatc ggcatttgcg
    15301 acgtattggc cgccggcaaa accgatttat gcacccccga acagcttgcc gaatttcatg
    15361 acaaagcggc aaaactgttc ccacccaaag ccaaagtggt cgaagtccaa aacgcacaac
    15421 tcgatatcca atggctcgat attcccgtag ttgaaaaatc acgctaccgc ctcaaagccc
    15481 tgccggacaa caccatgggc ttccagtcgc aaggtttcac attccccgcc ggacgcgatt
    15541 tcgacggcga aaaattgacc aacttcttca atgatttgcc caaaatgacc gaaggactcg
    15601 tccgcgccaa aggcgtgttc caagtattgg gaacgtgggt gtggctcaac tgggtggacg
    15661 ggcagtgggg cgcgaaccaa gtgtcttggc ggcgcgattc gcgtttcgag ctgattgcca
    15721 aatcgtttga cgcggattta atcgaacaaa aacttaaaga cgcattggaa tagcgttcac
    15781 gcgctttcag acggcatatg aaatgaaaat gccgtctgaa ggctttagga ggatgggatg
    15841 aaacccaaat tcaaaaccgt tttaaccgcg ctgcttttgg cggtttgcct gccgtctatg
    15901 gcggcaaccc gcgtcctgat ggaaaccgat atgggcaata tccgtttggt tttggacgaa
    15961 tccaaagcct ccaaaaccgt tgccaatttc gtgcgctatg cccgaaaagg cttttacgac
    16021 aacacgattt tccaccgcgt catcggcggc ttcgtcatcc aaggcggcgg attgaccgag
    16081 gacttggtgc aaaaggcaac cgataaggcc gttgccaacg aatccggcaa cggcttgaaa
    16141 aacaccgtcg gcactatcgc aatggcgcgg acggcagacc ccgattccgc cgccgcccaa
    16201 ttctttatca atctggcgga caacggctcg ctcgactaca aaaacggaca atacggctac
    16261 accgttttcg gcagggtcga aagcggaatg gacaccgttt ccaaaatcgc ccgcgtcaaa
    16321 accgccacgc tcggccttta tcaaaacgta cccgtacagc ccgtcaaaat ccgtcgcgtt
    16381 gttgtcgggc agtaacacgc agacagacgt tcagacggcg tcgcccgttt cccaaaaaac
    16441 gccgtttaaa gtaaaaaaat attttaaaac agacagttga tattgacaaa ttcaaaccga
    16501 ggattttaaa atgctgccaa cccaacccaa cccaacccaa cccaagccct gatctaagcg
    16561 acacagccgg ggcgaacact gaggcaatct acacttcaga cggcattacc gccaactcta
    16621 cccaactcga acagctcaaa aaactgtttc ccgcctgttt tgacgcagac ggaaatttcc
    16681 ttatcgacag attacaagcc gaaattgcgc cacagaccga catcggacgc gaattttacg
    16741 aaatgaactg gctgggcaaa tcatatgccc gcctgcttcg caacctgccg cccgaaaccc
    16801 tgataagtga agacaaaacg cacaacgcca agcccgaaaa tgcaggcagc caaaatctgc
    16861 tgattcgcgg cgataatctg gaagtgttga aacacttaaa aaacgcctac gcaaacagcg
    16921 tgaagatgat ttacatcgac ccgccctaca acaccggatc ggacggcttt gtctatcagg
    16981 acgaccgcaa attcacaccc gccgaacttg ccctcctggc gaatattgac gaagacgaag
    17041 ccgcgcggat tttagatttc accgacaaag gctcaaactc gcacagcgcg tggctgacct
    17101 ttatgtatcc gcgcctgtat atcgcccgcg aactgttaaa ggacgacggc gtgattttta
    17161 tctcgattga cgataacgaa gcggcgcagt tgaaattgtt gtgtgatgaa gtgtttgggg
    17221 aagggaattt tgttgcacaa ttgccttggc gaaaaagaac agctaaatca gatgtgcctt
    17281 ttggtatttc gcaggattat gaatggatat ttgtattcgc aaaatcttgc caatttattg
    17341 cagcaactaa aggcaaggaa cgacgctatt atgagactga tgatttcccc gatcgtcctt
    17401 ggcgtaccca cgacttaacg aaacaaacaa ctgcggcgga aaggccaaat agttttttca
    17461 caatggttga tcccaagaca ggaaaaaaat atccagcaaa tccaaatgca acttggcgtg
    17521 taaccaaaga tacatttcaa gattattata ataaaggaaa aatcgttttc cctgatgatt
    17581 atgatttctt aaatatcagc aatccagtta tgcgttactt taaagatgac gacatgaaaa
    17641 aagctggcga ggattttggc aaagtagctg taagcagtag attacctgaa aatgttggta
    17701 cattagccga tgcagtagcc gaatatttgg ctatttttag taggacgcta cctgaaaata
    17761 tcggaatgac taaagaaggc acaaaagaaa tcacagattt atttggtagc aaaatattta
    17821 ccttccccaa gcctagtcaa ttgattaaat ttttagtttc aataagttca aagagtaatg
    17881 acctaatcct agacttcttc gcaggcagcg gcacaaccgc ccacgccgtg atgcagctta
    17941 acgccgaagg acaaaacggt aaccgccgct atatctgtgt acagcttccc gaaaaaaccg
    18001 ctgaaaaatc cgaagcccgt aaagcaggct acccgaccat cttcgacatc accaaagccc
    18061 gcatagaaaa agccgccgcc aaaatccgcg tcgaacatcc cgattacacg ggcgattcgg
    18121 gcttcaaaat ctttcaaacg gcagacaatt tcaggcagca tccagacaag gatttttcgc
    18181 ccgaacaacc ggatttgccg cttaacgatg aattaagcga agaacagctg caaacgcttc
    18241 tgaccacctg gacgctgtat gacggggccg cactgaccac gccggttgag cctgtgcggt
    18301 tgggcagtta tacggcgtat ctgtgcgaaa aacggctgta tctgcttaac aagggtttta
    18361 cttccgccga tttgctggcg tttatcaaga agctggacga cgatgcggat ttcaatccca
    18421 accgtgtgat tgtattcggc agcaatatgg caagcgccat gcagcacgaa ctcgaccagg
    18481 cggttcgcgg ttataccaat aaaaaagaga ttgagttgaa tgtgattatc agggtgtaac
    18541 cggaatccga aagtgcaggc tgcttttcgg cttggattcc ccttgcgggg gaaggacgat
    18601 acaacaatga cttcatggag aaacattcat gagcggtttt aattacgaga aaaaccagcc
    18661 gcaccaaatg cgggcggttt cggcggtttt gggcgtgttt gacggggcaa cgcccaaata
    18721 tcggacggca gacgaaaatc ccgaactttt gtttgctgca aaacaatacg caaacaatat
    18781 cctgaaagtg caaagccaaa acggtataga cggccgattc cccgaccgtt cggacgacca
    18841 aaatatcctt gatatttcca tggaaacggg cacgggcaaa acctatacct acacacaaac
    18901 catgttcgag ctgcaccgtt ggctgggcgt gttcaaattt atcgtggtcg tgccgacttt
    18961 gtccattaag gcgggaacac agcagttttt gcaaagcaag gctttggcag agcattttga
    19021 acaggatttc ggcggcgatt atgaaggcgt acgcctgaaa acctatgtgg tggaaagcgc
    19081 gaaaaagaat aagggcaaaa agtccaatgc gcccataacg attgagcaat ttgtcaaagc
    19141 ggaaaacaaa aaggaaattc atgtgctgct gattaacgcg ggcatggtta attcgtcgtc
    19201 catgaacgat acgggcgaca aggcattgaa ggatttgttt gacaatcccg ttgatgcatt
    19261 ggctgccgtg cgcccgttta tgattgtgga cgaaccgcat aaattcccga cccgagatag
    19321 cgcgaaaacg tggggcaata tcaaacgctt aaaaccgcaa tatattttgc gctacggtgc
    19381 aacatttaac gatgaatatt acaacttgct ttaccgtttg acggcagtag acgcgtttaa
    19441 cgacgggctg gtcaaaggcg tgcgcgtgtt tcaggaagaa atgcagggcg gcatggatgc
    19501 ggcggtaaaa ctggtgtcgt cggacggcaa agaagcgaaa tttgaattaa acgaaaagga
    19561 caaaaagcag acgttcaaac tggcaaaagg cgaagatttg gcgcaaatcc atccggctat
    19621 ttcggatttg aaaatcgaca aaatgaataa aaccgtggtg gtgttaagca acggcttgga
    19681 gttgaaaacg ggtgccgtca tcaaccctta ttcctattcg caaacggtgc aggatgcgat
    19741 gatgcagcgg gcggttgccg aacatttcaa gctggaacgc gcgcttttgg cagaacgcgc
    19801 gccacagccc aaaatcaagc cgctgacgct gttttttatt gacgatatcg cgggctaccg
    19861 cagcggcaac gagctttcag gcagcctgaa agataaattt gaaagctgga ttcgcgcgga
    19921 agcggcacgc cgtctgaaaa cggaaagcga cccgttttac cgcgattacc tgcaaaagac
    19981 gttggacgat gtatccgcct gccacggcgg ctatttttcc aaagacaata cagacagcga
    20041 cgatagaatc gagcaggaaa tcaatgaaat cctgcacgat aaggaaaaac tgctgtcttt
    20101 ggacaacccg cgccgcttta ttttttccaa atggacgctg cgcgaaggct gggacaatcc
    20161 caacgttttc cagatttgca aactgcgttc cagcggcagc acgacttcca agctgcaaga
    20221 agtcggacgc ggcctgcgcc tgccggtaaa cgagctgatg gcgcgggtgc gcgatgtacc
    20281 gtacaaactg aattattttg tcgatagcag cgaaaaagac tttgtgaagc agcttgtcgg
    20341 cgaaatcaac gacaattctt ttcaggaaga aatctccaaa aagtttaccg aagagctgaa
    20401 acaaaaaata ttgcaaaaat accccgatat caaaccgctg gtattggtaa accaactgtt
    20461 ttcagacggc atcattgacg acaatgaaaa ctttgccgaa gacggctatg acaaattaaa
    20521 agccgcctat cccgaagcct tccccaaagg tttggacaaa ggcaaagtca gcaacgccaa
    20581 agacgagggc aaagacacca tcatcatgcg cgaaggcaaa tatgaagaac tcaaagcctt
    20641 gtgggagctg attcaccata aagccgtttt gcagtacaaa atcaaagatg aagccgaatt
    20701 tgtcgattta tttaccgcct atttgcgtga aaacgccgcc aaattcccgc aggcaggcat
    20761 atgcacggcg gtaaacgaag cttatatcaa caacgggctt atgctttccc gccgcataga
    20821 cagtattgaa gatgaagatt ttatccgttt caacacaatg acttaccgtg agtttctgga
    20881 aaaactggca caaacggcaa aaatccagat gcagactttg catcaggcgt tttaccgcgt
    20941 ccgcgacgaa ctgaacattg gcgatttttt gaatatgcag accatcgccc aaatcaaaaa
    21001 cggcttcaac cggtttttgc ttcatcattc cttccataaa ttcgaactgg attaccggct
    21061 tgtcggcagc aaaatccatc cgaccaaatt taccaataaa gacggcaaac cgcgcgcggt
    21121 gaaaaaagca gatttgggca gatttgaaga tacggagcac cggcctgccg ccggctatct
    21181 cttcggcgag attttctacg attcggatat agaacatgaa aatgtcgcca acaaccaaat
    21241 tgaaggcgta atcgtattta ccaaaatacc gagaaactcc atcaaaatcc ctgttgccgg
    21301 cggcggcacg tattcgcccg actttgccta tatcgtgaaa accaaaagcg gcgagattct
    21361 gaactttgtg attgaagcca aagggactga cggggcggaa gatttgcgaa aaagcgaaga
    21421 gcggaaaatc aaacatgccg aaaagctgtt tgccgagatt tccaaagaaa tcaaggtggt
    21481 gttcaaaacg cagtttgacg gcgagaggat agccgaactg atcgggcaaa atatgccagc
    21541 aggcgggcat tctgaaaacg gacactgaga aacaaatgtt tggaagccgt ctgcttcggg
    21601 attttaaaat gccgtctgaa acgccaaacg ggcttcagac ggcatttttg acggtggagg
    21661 tttacgcgcc gcaggttttc ggcttgttcg ccagaatgtt gatgactttg cgttcggctt
    21721 tttgcggctc gattttgatt tcgctctcat cttcttcgct gccgtctgaa aagcgttcgg
    21781 gcattttttc gctgtcaaaa gccaaatcgc cgccgtgttt cagattttga ccgcgttcca
    21841 atccggcaaa gtcaaagagt tcggtatcgg caaggtggga agggacgacg ttttgcaggg
    21901 cggagaacat cgattcgata cggccgggga agcgtttgtc ccaatcgcgc agcatatcgc
    21961 cgatgacttg gcgttgtagg ttgggctgcg agccgcagag gttgcacggg ataatcggga
    22021 actgtttcaa ttcggcgtat ttgattaagt ctttttcttt cacatacgcc agcgggcgga
    22081 tgacgatgtg ttcgccgttg tcgctcacca gcttgggcgg catggctttg agtttgccgc
    22141 cgtaaaacat atttaaaaac atggtggcga ggatgtcgtc gcggtggtgt cccaaggcga
    22201 ttttggtgca gcccaattct tttgcggtgc ggtagaggat gccgcgccgc aggcggctgc
    22261 acagcgaaca agtcgttttg ccttcgtcca acacgcgttt gacggtggag taagtgtctt
    22321 cttcaacgat tttgtaggga acgccgatgc tttcgagata ggtcggcaat acttcttcgg
    22381 ggaagcccgg ctgtttttgg tcgagattga cggcgaccag ttcaaaatca atcggcgcgc
    22441 tggcttggag ccggcgcagg atgtctaaca gggcgtagct gtctttgccg ccggagaggc
    22501 agaccatgat tttgtcgccc ggctcgatca tgttgaaatc gttaatcgtg tcgccgacgg
    22561 cgtggcgcag gcgtttgctg agtttgttgt tttcgagttc ttgcttggtt tttttggaca
    22621 tggcggtggt ttgaaaaatt agggatgcgg cattgtaacc gaaaacaggg gcggcaatgc
    22681 cgtctgaagg gcttcagacg gcattagagg gcttattctg catttttggt tttaaagaag
    22741 agatgaaccg ctttgaagat accgccgttt gggacggtga gttctttttg cgcggcggag
    22801 aatttaatca cggcaagggc ggtaaagttt tccgaatctt gaacgctgtc gagtacgatg
    22861 ccggcttctt cgccgtccgc cgtcagcagg atgcccgctt cggctgccga atttcccgac
    22921 aataccgcca agccgcgttt gacctgcccc cgatactggg cgcgcgcgat gatttcctgt
    22981 cccgggtagc agcctttttt gaagtgtacg ccgccgatga tgtgctggtt gagcatttgg
    23041 gcgacggcgg tttctttggt agccgcgcat atccacggat aaccgctgcg gatttcgtgc
    23101 agccgccacg cgttttcggc ggcagcgtca taagggggta gggcggtttc gggggcgatg
    23161 tgcaaaatgc cccgatggtg caggatgacg gaacagatgc cgtctgagac gcattcggag
    23221 gtaaaggcga gactgggttc ttgcgcggca agcggttcgg cggatgctgc caattccgcg
    23281 ccgacggcgt aatcttcaag gatttcaaaa acggctttgg cgcgcaacac aaacatccgc
    23341 aggcgtttga ccgttgcttc aagcaaatct tgcgccataa tcagcagcaa atcgtctcca
    23401 cggttgacga caatcatatt ggcgatgacg cggcctttgg gcgtgttgta agtcgcgtaa
    23461 cacgcctgcc cggcctgaag attattgatg tcgttggaaa gctgtccgtg caggaaggtt
    23521 tggcggtctt cgccgctgac gcgcgccacg ccgaaaaagg gcagtaaggt tttcatcatt
    23581 tgcgtactct gaaaatataa ggaaatctgt ttatgcagtt gccgcgtctc tcttcacggc
    23641 ggttattttg atttcgacgc gccactccgg acgggcgagc cgcgcttcca cgcaggcgcg
    23701 ggcgggcgtt ctgccggcgg caacccaagc gtcccacacg ccgttcattt ccgcatagtc
    23761 gcccatatca cgcaggtaga tgaccgcatc caaaacgtgt gccttgtccg agccgcattc
    23821 cgccagccag cggtcggttt gggcaagcac gtcggcagtc tgttcggcag ccgtttcacc
    23881 gttttcggga accatgccgg agaggaaaat caagccgccc gcgcaaaccg cttcggaata
    23941 gcggggcgtt gtgccgaaat accggatatc catatcggtt tccttcgata aaggggatat
    24001 atggtaacat tgcgcttgac cgatttccat gttttgcatg acgaaaaatg agtaaacaca
    24061 cttatccgat aacacctgcc gtacgcgttt tgcatgaaaa cggcatcgaa tttgaacctt
    24121 ttacctatgc ctatgaggaa cacggcggca cggcgcagtt tgcccgactg ttcggcaaag
    24181 acgaacactt ggtcattaaa accattgttt tgcaagatga aaacggtaag gggccgattg
    24241 tcttgatgca cggcgacaaa cagatttcaa cccgcaatct ggcgcggcat ttgggtgcga
    24301 aacacatcga acccgccgcg cctgcgcagg caaacaagtg gacgggctat ctggtcggcg
    24361 gcacaacgcc gttcggcatc cggacaaaat tggatattta cgtcgaacag tcggtgatgg
    24421 atttggaaac catctatatc aatggcggaa aacgcgggtt cattatcggt atccgccccg
    24481 atgatttaaa tattttgaac ccgaaaacaa tacaggcagc ggtttgaccg ggaagtataa
    24541 ggagcaatat ggacaaagat ttgtatgccg tattgggcgt gtcgccgcag gcgggggcgg
    24601 acgaaatcaa acgcgcctac cgcaagctgg cgatgaaata ccatcccgac cgcaatccgg
    24661 gcaatccgca ggcggaagaa aagttcaaag aaatccaacg ggcttacgat acgctttccg
    24721 acctgtcaaa acggacgcaa tacgacgcgt ccttcagacg gcatgaggaa cgcgggcggc
    24781 aggaagaggc attccgccgc gaacaggcgc gcagggagca gttttacggc gaacagatgc
    24841 gccgcgaaca ggcgttcaga cgggagtttg aacggcaggc ttcacgttcg cgccatgctt
    24901 acgaaccgtc cggcagcgga agcgggcgca actatatcct cgccgcctac atcctgttcg
    24961 gtttgggtgc aatcatgcag ttcatgccca tagtcggcgt gattctcgcc tatatgaaac
    25021 ggaacagttt ggacagcatt gtctatgccg cacataccga atacctgatt aaaacctttt
    25081 ggcgcacatt ttggctttat cttttgggcg cgctgactgc ccgtttcggc atcggcgtgc
    25141 tgattattat tgcaacgacc gtctggtatt tctaccgcat catcgccggc tttatccgct
    25201 tcaacgacgg cagggcggtt gcgcccgaga aatggatata gagcatggcc tacctgttaa
    25261 tcagcatcgt gttcagcgtg tcggtttcca ttttgctgaa aatggcaagg aagaaaaaaa
    25321 tcgacatcgc gcaggcggtc gccgtcaatt atgtggtcgc ggtcatactg accctgtcgg
    25381 tattgaagcc ggatgtcggc aatatcggtg catttttgcc gacgtggccg ctgtttgccg
    25441 ctttgggcgt gctgctgccg tccgtatttg tcattatggg caaatccgta gaatccgccg
    25501 gtatcgtcaa atccgacgcg gcgcagcgtt tgtcgctgtt tttaccgatt gttgccgctt
    25561 tgacgctgtt tggcgaaaaa ctcagtgaag gcaaactgat cggactgtgc ctcgcctttg
    25621 ccgcactgtt ctgcctgctt tggaaaagcg acagcggcaa aaaatcaggc agcgcgtggc
    25681 ggcaggcgtt attgctgctc ggtgtgtggg cgggttacgg cattatcgac atcctgttca
    25741 aacagattgc caaaagcggc acggcatttg cgggcaacct gctggttgca tttgcgctgg
    25801 cgggtgtgct gatgtttgcc tgcctgtttg ccaaatcggt cagatggcgc gttgagagcg
    25861 tggtcggcgg catactcttg ggcggtttga attttatgaa catcgtaacc tacatcactg
    25921 cgcaccaaat gatgaaggac agtcccacgc tggtttttgc cggtatgaat atcggtgtga
    25981 ttgttttagg gacgctttcg ggcgcattgt tctttaagga aaaaatcaac acaatcaata
    26041 cggcaggcat tgtactgtca ttatgctcca ttgcctgcct gttctattgg acagaagtta
    26101 aggcactgtt cggcatataa tccggctgat tgatataaca aaatgccgtc tgaaacagga
    26161 aaatgctgct tcagacggca ttttgttata cgggcagatt caaaatttaa ttaataatag
    26221 ttatttttaa ttataattta aatattaata ataattaaaa atttcaaata attcttatta
    26281 tgagtgtatc ttaataaaat tcattttata ttcttaataa ggaatacatg atgtccggat
    26341 tctcccccaa acctaagacc attatcctaa gccttgcagg tgcatttggc gcattggctt
    26401 ttgcagacac cccgaacaat accgaacagc agaaagaact gaataccata gtcgtccatg
    26461 gcaaacgcag tgccgaccaa aaaggcgcgg acgacgtgta ttacaaaaac gtctccaacg
    26521 cctacgtcgg caaagaatac ctcgaacgct accgcgtcca atccgccggc gacgtactca
    26581 aaggcctaaa cggcgtgtac aacatgaaca cccgcaccgc cggcggcgcc atcacgccca
    26641 acatacgcgg tatcaccggc aagggccgca tcccggttac catagacggc accgaacaaa
    26701 ccatcgacgt atggatgaac aactacggcg tgggcgaccg caactacctc gaccccgccc
    26761 tgttccgcag catcgcggtg gaaaaaagcc ccgccctcac gcgcggcgtg aaatcgggcg
    26821 tcggcggcgc gatgtccatc cgcaccatcg aagccggcga catcgtgccc gaagggcaga
    26881 aattcggctt ccagctgaaa accgagtttg ccaacaacag aacccgcccg gccaacaacc
    26941 tcaaccaatg gctgggctgg gaagactacc gcaccctgcc gctcggcgcc accgccgacg
    27001 gcgcgggcgg cggttctgac ccgatcaccg gacagcagag cccgcaggcg ttggtggtag
    27061 acaactttac cccgccgcag cacaaaagcg gccgcgacaa ctggcgcttc ggcggcgacc
    27121 gcagttatat ggccgccgcc gccttcaaaa ccgaactttc agacggcctt gccgcctaca
    27181 gctaccgcaa caaaggcaac tacttcgccg gtcgcaaggg tgcggaaggc tacctgaata
    27241 atccggtata cgacctgcaa aaatgctacg acgaaggcgg cagcgatttc aactgcaaaa
    27301 attccgccac cttcatcccc aatatggcgc gcatctacca ccccggggtg gaagtgctca
    27361 acagcaacac cgaaaccaaa accctgctgc tgaaaaacaa ctggcacctg cccggcagcc
    27421 atcggctcgg ctggcaatat atgcgcaccg acgtgcgctt cggagagatc aaccccttcc
    27481 acaccaccta tgtgatgaat atggaagagc acaacgagac caaccgcccc aaagaactgt
    27541 cgccacaggc gcagagcatc gattccacca tccgcaccga cacctacaaa ctcggctggg
    27601 cctggaagcc ggaaaacagc cgctgggtcg atctgcaggc caacctgtgg cgcatcaaaa
    27661 ccaacagcac ccgccaccag agtggcggca tggatttgtc ttccgcccgc cccgatccct
    27721 tctacgacgt ttggtattgg tgtacccaac gcggccggat accgcccgag cacgtcgaca
    27781 actacagcag ctgcaacgac ctgatgaatg atttcggcgt caacggcctg accaaagaac
    27841 aggtgttggc catgaccccg aacgacaacg gccaattccg cgtgatttcc ggcgccgagc
    27901 agaaaacccg cgtcagccgc accggtttcg atatcagcaa ccgcttccgc ctcagagacc
    27961 gcctgagcat gaccttggcc gccgattatc agaaagagaa actggcggag gaagtggaaa
    28021 tcgtcaattc gcaagacctg ttcaacctgg ccggcatggt aaccggcctg accaagctgg
    28081 ccgggccgcg cggcggcgag cggcgcgaat ggggcaccaa tttggtgttc gactggcagg
    28141 ccgccgaccg cctgaaaatc tccgccggca tccgctacca caacttccgg ggcttcgata
    28201 ccgccctggc cgaagggcgt gcccgccgcg acccgcgcta tcaggcaggc gatggaaccc
    28261 agcattatgc cgatggtgcc tatctgccgt atttcgaact ggcaggcgat caggagcggc
    28321 gtgactggaa tgtggtggcc gaacagcttc ggcaggcgta tcagtcaggc gatgctgccg
    28381 ccattgccgc ggcagtacag gcagaacagg cgcacggcgc ccgttaccat ttgctgaaat
    28441 actatgacgg ctcgtatcgt accgatggtt atgtgtatat cggcaacgat ggccgtgcct
    28501 attttgacgg acggttcggc ggggttaacg aggcacagaa ccaaccgctc tatcgggtgc
    28561 gccccgttta tgtgccgttt gtcaacggca agctggacag cggcgcattg ccccagcatt
    28621 ttcatgattt cgtcagcaat tatcaggaaa aagtgaccaa tccgcagggg ctgcacggga
    28681 cgcattatcg atattgggca ggcgtgggga atcctatcga ctgtataaga agtgatgcgg
    28741 cctcttgcct tgctcataac ctatcgggaa aagtaaccaa ttttcaagtc ggctatcccg
    28801 agtacggtga tgataccggt ctcggtctca aaatcatcgg tcggcactat accgaggaac
    28861 agtattgggc gcagcccaaa ccgatccgtg cccatgcctg ggcgccgacc atcgccgtca
    28921 gctacgatct gaccgacaac agccgcctgt tcgtacgcta tgcgcagatg agccgcttcc
    28981 ccagcgtgta tgaggtgggc agtttttata acgacgtggc ctatgccggt atgccgaagg
    29041 ccccaaattt ccgcttcaag ccggaacgca gccgcagctg ggaaatcggc tatagcttca
    29101 attttgcccc gtattggagc cggctgcgtg ccggcgatat gcgcctgacc tactaccgca
    29161 accgtatcca aaatgtgatc gaaaccacag attatttccg caccgtgcaa tacgaccgca
    29221 aggacaccgc cgggctggaa tggcagagcc gcatcgacac cggccgcttc tttgccgcgc
    29281 tcggtgccac ctaccgcctg aaacagcaga tgtgtgaccg cgataccgct ttcgattacg
    29341 acccttatgg tgccaaaggc gtgccgatct gcatcgaagg cggctacggc agtacgcgcg
    29401 gctatcaggc cttgcagccg aaatattcaa taaacctgga tgccggggtg cgcctgttgg
    29461 gcgagaaact ggagctcggc ctgcgcggca tctaccacag ccgggtcaac accaagcaat
    29521 acgaccaact gctgcaaaag gatttgggca tcatcttcga taccaccggc aagccccacc
    29581 attggcggtc atcgctgact tgggatgttt atggccgcta ccggctgcac aaaaacctca
    29641 acgtcaacct cggcatcacc aacctgacca accgctacta tctcgacccg atgtccaacg
    29701 ttgccgcccc cgggccgggc cggacggtta ccttcgggct gactgccaag ttctagccgc
    29761 agggcggagt ggggttttca ggtagcctgc gggtatggcg ggaaggctac ctgaaaacgg
    29821 attggatata acaacaaaca attgaacact ccggacgcac aaacccaacc ggccccgctt
    29881 atccgaggta atggttttta ccgttttttc cgccggttcg gcggtaataa aacggtggct
    29941 tttgtttaac tcttaattaa ggaaacgatt atgtctatac gtaataaaac cctgttggcc
    30001 gtcttgctgt gcggcgtttt tggtgtggct gcggctgctc cggctggtgg ttccagccaa
    30061 aggaaaagcc aagatccaaa tagtgacccg acgacagttc gccccggcac ctctgacaag
    30121 caacctttcg gtgccggtaa ggccggtttg gcattcagta tcaataacgg tggaagttgg
    30181 tcgaaatcca tcaatctgga tgatggtccg gtcaaatata tccgtgcgaa atatcaggca
    30241 ccgattaacg gcaacgatcc ctatgctaat gttgatggta cgcgcaatct gcaatatctg
    30301 cgcttaggcg atgtgaaacc ggttatcggc tggttctaca acccaacgtt gggtcaggtg
    30361 tggtatgaaa accaccacaa caacactgaa gtgtacagtg tgcgccagat agccaacccg
    30421 gcaatccccg ccgcacccaa gttcggcggc ctggtaatcg ccaaagtgcc gggcatgggc
    30481 ggcagcaacg gcaacgtctt cttcggcgaa tgggcgccgc gtgccggtaa cccgcagcaa
    30541 atcagcaccg acctgaatat ggcggatggt aaacgcaccg tttggtatgt gggcgataat
    30601 cccaccggca acaccaccgg tgtgagaacc gccacctaca atgtgctggg tatcaataag
    30661 cacaccccgg ggcagaacga tttctatacc ggtcaggtaa ccgccacctt cggttctggt
    30721 ataaccggga cgatggcagg cagaatcagc cgcagttccg atcacgtcga ttttgatcac
    30781 gccaaaatcg ataacagaaa tggtaccttt gccgatccca gtcaaggcat gaccggccgc
    30841 ttctacggca accaggcggc agccatggcc ggctatacca cacgtggtac cggtaatgtc
    30901 ggcgatgacg tagctttcgg cggtagcgcc accgatattg agacccgcaa ataacagctg
    30961 ccatccgatt tggtttggca acggctaggc tggatttaac ccctgaggag tttcaagctg
    31021 ccgttttatg ccgactgcca agccatataa atcatccgtc cgatacggat tgctgctcgg
    31081 tttcaggtag cccgtgttca acacaggcta cctgaaattt gatatttgta aataaacatc
    31141 gcctatccca tacggtttcg gcctatcgga cattttggga cagcaattaa aacctgcgtt
    31201 actttatctt ttcggaaggc aaccatgaac aaaccactca gcctcgccct cttctgcctg
    31261 cttctgccca tcaccgccca cgccgacaac cccgccgacg aacgccgccg cctgctggac
    31321 gagggcagcc gccaaacgca gcaataccgc gaaagcggtt ggctggacac ggaacaggca
    31381 cgcggggaag tagaggaaaa cgacggctat atttcgatag gcggcgaaat ctaccaagtc
    31441 ggcgatacgg cggaagaact ggaaagcgca atttaccacg ccctcaatgc gcggcagtgg
    31501 cacaaggtgc ggcagttcgc cgcccgctac gccaaactgc cccggcacaa acccgcgctg
    31561 attcatttgg ccgatgcgct gcaaaaacgg gacgaagggg atttccgcgc ggcggggaac
    31621 agttttcaga cggccttgga agccgaaccc gacaaccccc gcctgctgct cgaagcgggg
    31681 cggttttatg ccgaagacaa ccaaaacaaa gagtctgccg ccgcgtttga gaaagttctg
    31741 aaaacggaca ttcccgcaga aacccgcccc atcgtggaaa actacctgtc cgaactgggc
    31801 aaacgccgcc gctggcacgg ccaaatcagc ctcggctacg gctataacag caatgtaaac
    31861 caaggcaacg gcatcaacca atgcgtgtgg gaaattgcgg gcatgtgcct gatggagcgc
    31921 accctgcccg cgccaacgga ttcgaccttc tcatcctata gcgcgaccgc cgaaaaaacc
    31981 gtcccgctca aagggaatca cggcgtacag gtgcgcggtg tactgtacgg caaccgctac
    32041 acggaaaaag acaaagattc ggcggctatg ccggattacg gctatcgcaa cggttcgctg
    32101 tacgcgggat acgcctatgc cgacgcacgc tcctcctttt ccctgctgcc ttatttcgaa
    32161 tacgatttcc gcaaccgcca cacccactac cgagcatggg gtgcggatgc cgactggtcg
    32221 cgcacactct ccccgcactg gcgcattaac tcacatgccg gagcaaaaaa gaccggatac
    32281 ggcggacaaa gcaaaactta ttttgccgat ttcaaacaat acgaactcgg cgcgggggcg
    32341 gaattttcca tcacgctgaa aagcggcctg cttgtaaatt tcgatgccgc acgcaaagcc
    32401 tatcccgaaa aatcctcttc cagcaaagaa tatacggctc ggctgggcgc gtataggctt
    32461 ttttcgggcg gtacttattt aaacgccgtc ctgctctacc gccgcagcct ttatgacgcg
    32521 gcaagtttcg tcagcgataa caaacggcgg cgcgacaagc aatacatcat gatggctgcg
    32581 gcgggttttc cgcaatggaa tatcaaaggc gtatatcccg aactgcgttt caggcgaaca
    32641 atcgcacaca gcaacgcggt gtattaccgc taccgccaaa acgaatggct gttgggtttt
    32701 aaatatcggt tttgaccagt cgggtgataa aaaatgccgt ctgaaagggc gcttcctttc
    32761 agacggcatt tgtccgtaat gtttacagat gaggattcat caggttctcg ggggagagga
    32821 tgcggttgag ttcttcttcg ctcaacaacc cgcgttccag tacgacttcg cgcacgcctt
    32881 tgccggtttg ggcgcagatt ttgccgacca agtcgccgtt gcggtggccg atgtacggat
    32941 tcagataagt caccagcccg atggagttga aaacgtaacg ttcgcagatt tcgcggttga
    33001 ccgtaatgcc tttgacgcat ttgtcggata ggttgactgc ggcattgccc aagagggaaa
    33061 tggtttcaaa catacattgg gcgatgaccg gctccataac gtttaattgc agttgcccgg
    33121 attcggcggc gaaggtaatc gtcgtatcgt tgccgatgac tttgaagcag acttggttga
    33181 cgacttcggg aatcacggga ttgactttgg caggcatgat ggaagaaccg gcctgcaatt
    33241 cgggcaggtt gatttctttc aaaccggcgc gcggaccgga agagagaagg cgcaaatcgt
    33301 tgcagatttt agagagtttg acggccgtgc gtttcaatgc gccgtgtacc atcacatatg
    33361 caccgcagtc ggaggtcgct tcgatcaggt tttcagtcag tttgcaaggc aagccgctga
    33421 cttcggagag ttttttgacc accagttcgg catagccttt gggcgtgttc acgcccgtgc
    33481 cgattgccgt tgcgcccaaa ttgacttcca agagcagttg gcgggtgcgg tcaaggttga
    33541 ggatttcctc ttccaacaat acttggaaag attggaattc ctggcctgcc gtcatcggta
    33601 cggcatcttg aagctgggtg cgacccattt tcaaaacgtc tttaaacgct tcggctttgg
    33661 cggcaaaggc gtttttcaat acggtcagtt tgtcgagcaa ttcgccgatg ctgtaataca
    33721 cggcaaggcg gaagcccgtg ggataggcat cgttggtgga ttggctggcg ttgacgtgat
    33781 ccatcggatt gacgatgtcg tagcggcctt tctcatagcc caaggcttcc aatgcaaggt
    33841 tggcgatgac ttcgttggtg ttcatattga ccgaagttcc cgccccgccc tgatacacgt
    33901 cggacgggaa ttggtcgagg cagcggttgt tcagcagaac ttcgtcgcaa gccttttcaa
    33961 tggcggcggc gatttcgggt tttactgcac ccaattcgcc gtttgcctgc gcggttgctt
    34021 ttttcaccat caccatactg cggacaaact gcggcacgtc ggaaattttt tgtgtggaga
    34081 ttttaaagtt ttcaatggcg cgcagggtgt ggatgcccca atacacttcg gcgggaatct
    34141 cgcggtcgcc caataaatcg tgttcgatac ggacagtcat gtttttacct ttgtaagtcg
    34201 gataattaat attgaaaaaa tgcgccatcg gaaagatgcc gcagcaggac gaacactata
    34261 ccggccggat gaaattgtcc atatcgtatg ccgtctgaaa acgggaaacg ttgttttcgg
    34321 tcgttacttt ccggactttc cggcaagccc cgcctgccgc caaaatatct ttcggcgggt
    34381 tgtgctccgc catatcggct tacggttggc agggcggttt gatgaagacg gcatttgtgc
    34441 tgattcgcat caagaggctt attgttttgc agcttcgatt tggatgtcga tgcggacgtt
    34501 tttggtcata ccggcgttaa cgaggtagtc cacgccccat ttggtgcggt cgatggtggt
    34561 gctgaagtcg ccgccgcaaa cttcggtttc cgccatcggg ctttggtagc agttgaattt
    34621 ttcggctttg agtttgacgg gggcggtttt gccgcgcatg gtcaggttgc cgtcaacgga
    34681 aacaagtttt ttgccgttga agttgaattt ggtggaaacg aagcggatgt ccggatattg
    34741 agcggcatcg aagatgtcgg cggatttcag gtggccggtg aagtgttgcg aaccgctttg
    34801 caggttggcg acgggaatgg tgatgtcgat tttgccgtcg cgttttgctt gatcgaactc
    34861 gacggaaccg gtcagaccgt aaaaaccgcc gacgttggtg ctggtgttga agtggtcgat
    34921 ggcgaaacgg acgttggcgt gatattcgtc cactttgtag gtggcggagg cagtgccgac
    34981 ggctgccgct gcgagcgcgg cgaagatgat ttttttcatg atgataatcc tttgtgtggg
    35041 ccggtaaagg cgtttatcct aacataggcg gggatttatg gcatttcctg ccggtaaacg
    35101 gtgggtttgc aggcggttga cgaacgggcg ggcggaacag ggcggatgaa aaagcgcggt
    35161 gttatccgtg ctttgttttt gggtctgtac cagatt
//
LOCUS       DAXDZG010000015        34444 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXDZG010000015 DAXDZG010000000
VERSION     DAXDZG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.015"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(172..1062)
                     /gene="sucD"
                     /locus_tag="ACFB78_000628"
     CDS             complement(172..1062)
                     /gene="sucD"
                     /locus_tag="ACFB78_000628"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC1268679.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1073..2293)
                     /gene="sucC"
                     /locus_tag="ACFB78_000629"
     CDS             complement(1073..2293)
                     /gene="sucC"
                     /locus_tag="ACFB78_000629"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC1268680.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            complement(2311..2598)
                     /locus_tag="ACFB78_000630"
     CDS             complement(2311..2598)
                     /locus_tag="ACFB78_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268681.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(2718..4151)
                     /gene="lpdA"
                     /locus_tag="ACFB78_000631"
     CDS             complement(2718..4151)
                     /gene="lpdA"
                     /locus_tag="ACFB78_000631"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1268682.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(4412..5593)
                     /gene="odhB"
                     /locus_tag="ACFB78_000632"
     CDS             complement(4412..5593)
                     /gene="odhB"
                     /locus_tag="ACFB78_000632"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC1268683.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(5693..8521)
                     /locus_tag="ACFB78_000633"
     CDS             complement(5693..8521)
                     /locus_tag="ACFB78_000633"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC1268684.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYVGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(8726..10009)
                     /gene="gltA"
                     /locus_tag="ACFB78_000634"
     CDS             complement(8726..10009)
                     /gene="gltA"
                     /locus_tag="ACFB78_000634"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC1268685.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10121..10369)
                     /locus_tag="ACFB78_000635"
     CDS             complement(10121..10369)
                     /locus_tag="ACFB78_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC1268686.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(10373..11080)
                     /locus_tag="ACFB78_000636"
     CDS             complement(10373..11080)
                     /locus_tag="ACFB78_000636"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC1268687.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11200..12963)
                     /gene="sdhA"
                     /locus_tag="ACFB78_000637"
     CDS             complement(11200..12963)
                     /gene="sdhA"
                     /locus_tag="ACFB78_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC1268688.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(12966..13307)
                     /gene="sdhD"
                     /locus_tag="ACFB78_000638"
     CDS             complement(12966..13307)
                     /gene="sdhD"
                     /locus_tag="ACFB78_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC1268689.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13301..13678)
                     /gene="sdhC"
                     /locus_tag="ACFB78_000639"
     CDS             complement(13301..13678)
                     /gene="sdhC"
                     /locus_tag="ACFB78_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC1268690.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(13965..15368)
                     /locus_tag="ACFB78_000640"
     CDS             complement(13965..15368)
                     /locus_tag="ACFB78_000640"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1268691.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(15617..16354)
                     /locus_tag="ACFB78_000641"
     CDS             complement(15617..16354)
                     /locus_tag="ACFB78_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC1268692.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(16633..18909)
                     /gene="metE"
                     /locus_tag="ACFB78_000642"
     CDS             complement(16633..18909)
                     /gene="metE"
                     /locus_tag="ACFB78_000642"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC1268693.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19045..19926)
                     /gene="metF"
                     /locus_tag="ACFB78_000643"
     CDS             complement(19045..19926)
                     /gene="metF"
                     /locus_tag="ACFB78_000643"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC1268694.1"
                     /translation="MQNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSI
                     HRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIA
                     KDYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKS
                     AQADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQ
                     LGKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTL
                     NRSELTYAICHILGARP"
     gene            20117..20392
                     /locus_tag="ACFB78_000644"
     CDS             20117..20392
                     /locus_tag="ACFB78_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC1268695.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            20392..20517
                     /gene="ykgO"
                     /locus_tag="ACFB78_000645"
     CDS             20392..20517
                     /gene="ykgO"
                     /locus_tag="ACFB78_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC1268696.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(20554..20820)
                     /locus_tag="ACFB78_000646"
     CDS             complement(20554..20820)
                     /locus_tag="ACFB78_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268697.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKMPSETHFSVSDGIFEKGLR"
     gene            20963..22102
                     /locus_tag="ACFB78_000647"
     CDS             20963..22102
                     /locus_tag="ACFB78_000647"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC1268698.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22099..22680
                     /gene="metW"
                     /locus_tag="ACFB78_000648"
     CDS             22099..22680
                     /gene="metW"
                     /locus_tag="ACFB78_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC1268699.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            22797..23600
                     /locus_tag="ACFB78_000649"
                     /pseudo
     CDS             22797..23600
                     /locus_tag="ACFB78_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            23592..23699
                     /locus_tag="ACFB78_000650"
                     /pseudo
     CDS             23592..23699
                     /locus_tag="ACFB78_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23775..24335)
                     /gene="efp"
                     /locus_tag="ACFB78_000651"
     CDS             complement(23775..24335)
                     /gene="efp"
                     /locus_tag="ACFB78_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC1268700.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(24378..25529)
                     /gene="earP"
                     /locus_tag="ACFB78_000652"
     CDS             complement(24378..25529)
                     /gene="earP"
                     /locus_tag="ACFB78_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC1268701.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPGAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(25548..25826)
                     /locus_tag="ACFB78_000653"
     CDS             complement(25548..25826)
                     /locus_tag="ACFB78_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268702.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26177..27118
                     /gene="miaA"
                     /locus_tag="ACFB78_000654"
     CDS             26177..27118
                     /gene="miaA"
                     /locus_tag="ACFB78_000654"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC1268703.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEIYYLTGRPMSAHLNGQPEHTLPFELYTAALIPEDRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27122..27841
                     /gene="tadA"
                     /locus_tag="ACFB78_000655"
     CDS             27122..27841
                     /gene="tadA"
                     /locus_tag="ACFB78_000655"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC1268704.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMPQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            27846..28334
                     /locus_tag="ACFB78_000656"
     CDS             27846..28334
                     /locus_tag="ACFB78_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC1268705.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            28400..29152
                     /gene="rlmB"
                     /locus_tag="ACFB78_000657"
     CDS             28400..29152
                     /gene="rlmB"
                     /locus_tag="ACFB78_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC1268706.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEGILENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCNLPDSTAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29214..30589)
                     /locus_tag="ACFB78_000658"
                     /pseudo
     CDS             complement(29214..30589)
                     /locus_tag="ACFB78_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            30969..31844
                     /gene="dapA"
                     /locus_tag="ACFB78_000659"
     CDS             30969..31844
                     /gene="dapA"
                     /locus_tag="ACFB78_000659"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC1268707.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFAVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEG
                     GQAKVRTALKASGQL"
     gene            31853..33049
                     /gene="bamC"
                     /locus_tag="ACFB78_000660"
     CDS             31853..33049
                     /gene="bamC"
                     /locus_tag="ACFB78_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC1268708.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTERPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33106..34038)
                     /gene="pip"
                     /locus_tag="ACFB78_000661"
     CDS             complement(33106..34038)
                     /gene="pip"
                     /locus_tag="ACFB78_000661"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC1268709.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRSRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8525 a         9311 c         8520 g         8088 t
ORIGIN      
        1 gagtgttgta ctattttgtt tttaatccac tatattctga ctgcctcaaa gggcaaaaat
       61 aatgtgagta tgttgaggct gcctcttttt gaaatgacaa acaaaatgcc gtctgaaacc
      121 cgatttccgg tttcagacgg catttgatgt tcaatcagtt gtcaaacctg attatgccaa
      181 accttttgct ttcaacactt ccagcatcgt cgtgcccaac tcggcagggc tgcgggtgta
      241 ggcgataccg gctttttcaa acgcggcgaa tttttcttcg gcagtacctt taccgccgga
      301 gataatcgca ccggcgtggc ccatgcgttt gcctttagga gcggtaacac cggcgatgta
      361 accgacaaca ggtttggtta cgttggattg gatgtattcg gctgcttctt cttccgcagt
      421 accaccgatt tcaccaatca tgatgatggc atcggtatct gggtcttctt ggaagagttt
      481 cagtgcgtcg atttggttca taccaggaat cgggtcgccg ccgataccga tacaggttga
      541 ttgacccaag cccagtttgg tggtttgtgc cacggcttcg taagtcaatg taccggaacg
      601 ggaaataata ccgatgcggc ccggagtgtg gatgtggccc ggcataatgc cgattttgca
      661 ctcgcccgga gtaatcacgc ccgggcagtt agggccgacc aaacgggtac cgttgccgtt
      721 ggtttccaag taacgtttgg ctttgagcat gtccagagtt ggaacgcctt cggtaatcac
      781 aacgaccaag cctacgcctg aatcaacggc ttcaacgata gaatccaaca caaacggagc
      841 gggaacgtaa atcacggatg cgtcagcgcc ggtttcttta acggcttctt tcatggtgtt
      901 gaatacaggc aggtctaggt gggtttgacc gcctttgcct ggggtaacgc cgccgacaac
      961 tttagtgccg taagccagag cttgttcgga gtggaaagta ccgtttttac cggtgaaacc
     1021 ttgaaccaat actttggtgt ctttattaat caatacgctc attcttttct ccttaggcgt
     1081 ttacggctgc aacaattttt tcggctgcgt cattcagacc gtctgcagaa gtcagtttca
     1141 gacctgattc gttcaggatt ttcgcgccga gttcggcgtt gttgccttcc aaacgaacaa
     1201 cgacaggaac gttgacgttg atttctttaa cggctgccac gatggcttcc gcaatcatgt
     1261 cgcaacgtac gataccgccg aagatgttga tcaatacgcc tttaacggat ttgtcttcca
     1321 aaatcagttt gaacgcttca accacgcggt ctttggttgc gccgccgcca acgtccaaga
     1381 agttggcagg ttggccgcct ttgagtttga tgatgtccat ggtggccatc gccaaaccgg
     1441 caccgttaac catacagccg atattacctt ccagggcaac atagttcagg tcgaattcag
     1501 aagctttcag ttcacgttcg ttttcttgag atttgtcgcg caattcagcg atttttggca
     1561 ggcggtagag cgcgttgctg tcgatgccga ttttgccgtc cacgcaagcc agtgcgccgt
     1621 tttcgcgaac tgccagcggg ttgacttcaa acagggcgaa gtcgttttcg acaaacgctt
     1681 tgtacgcgcc ggtcatcagt ttggcgaact cgttgatttg tttgtctttc aagcccagtt
     1741 ggaaggcaac ttcgcgagct tggcaaggtt gcaggccgac cagcggatca acggttactt
     1801 tgaagatttt ttctggagtt tcagcagcaa ctttttcgat ttccacgccg ccttcggtag
     1861 aagccatgaa tgtaacgcgg cgggtagaac ggtcaaccac tgcgcccaag tacagctcgg
     1921 tttgaacagg gtacatgtct tcgcaaacca aaacgctgtt gacaggttgg ccgttggcat
     1981 cggtttggta agttaccaag ttggtgccaa tcaggctttc agccacttct ttggcttttt
     2041 cgcggctttt aacgactttt acgccgcccg ctttaccgcg gccgccggcg tgtacctgtg
     2101 ctttgacaac agcgaatttg ccgcccaatt tgtcgtaagc tgcagcggct tcttcgccgt
     2161 tgtgcgccaa aataccgcct tgtacgggca aaccgtagct agccagcagt tctttagcct
     2221 gatactcgtg taaattcatg gatttctcct taaggtgagt gggttttagg aatcgccctg
     2281 cggccaatcc catattttca gacggtccta ctatttactg aagggcaacc acatccgcgc
     2341 cagcagatac atgactgcac cgcctccccc gagaataaaa aagacgatgc cttttttacg
     2401 cgaattcgga cagagcagat aaacggcaag gctgaaactg ataaacaaca atgccacgcg
     2461 ccaaaccaat tctttatctt tgaaacggtt gagcgtataa gtttcggtca tcatcacata
     2521 catctgccac aatgcagcca tacacatgct gatgatgccc atcggtataa agaaaatacc
     2581 gactatttct ttgttcataa tgaatgtaaa tgctgtttgt ctgacggaat tataaatcag
     2641 aaaacgaagc tctcggaaca ttcaatttgc cggaggcctt aaggccgtct gaaaatttca
     2701 gacggcctta atgtctatca accgtgcaaa gcgcgtttgt cggccgccaa tgcagcttcg
     2761 tgaaccactt cggacaaggt tgggtgggca tggataatac gggcgatatc ttcgctgctg
     2821 gcgaagaatt cgagcgcagt cacgccttcg gtaaccaatt cgctgacaac cgggccgatc
     2881 atgtgtacgc ccaagatacg gtcggttttg gcatctgcca acactttaac cgtacctttg
     2941 gctttgccca ttgccaatgc acggccgttt gcaccgaaac ctgaagtacc ttttttgtac
     3001 tccacgcctt cggctttgag ctgctcttcg gttttaccca cccaagcgat ttcagggtcg
     3061 gtgtaaatca caaacggtac gttgttgaag tcgatatgcg gtttttgacc ggcaatgcgc
     3121 tcggcaacgg caacgccttc gtcgctggct ttgtgtgcca acatcgggcc gcgaaccacg
     3181 tcgccgattg cccatacgtt aggcaggttg gtacggcatt cgccatccac tttgataaag
     3241 ccgcgctcgt ctttttccaa gcctacggct tcagcgttca ggcctttggt gtttggaata
     3301 cggccgatgg caacgatcag tttgtcgaat acttcggttt tggcttcgcc ggcagcagtt
     3361 tcgtaagcaa cggaaacacc tttgccttca gacttgatgt cgccgatttt cacacccaat
     3421 tcgatgctca gaccttgctc tttggtgaag tatttgaagg cttctttggc gatttgttgg
     3481 tcggcggcag ccaggaaggt cggcgcggct tcaagaatgg taacttccgc acccacgcgg
     3541 ttccatacgg aacccatttc caaaccaatc acaccggaac cgattacgcc gagtttggca
     3601 ggtacttcgg tcaggttcaa tgcgccttcg ttgtccaata cgttcacgtt gtcgatagcg
     3661 acttgcggca acggacgcgg tacggaaccg gttgctacga tgacgtgttt ggcttcgata
     3721 acggttttct cgcctttgtt atcgacttca atttggtaag catcgccatt tttaccggca
     3781 aaggaagcag taccgaacag gctggttact ttgttttttt ggaacaggaa tttcacgccg
     3841 ccggtcagtt tggtcacgat ggcatctttg cgctcaatca ttttggccac gtcaaatttt
     3901 acgtcgccga cagtgatacc gtgttcggca aaatcgtgtt gcgcagcgtg gaaatgttcg
     3961 ctggattgca acagggcttt agaagggata cagcctacgt tcaagcaagt accgcccaat
     4021 gcaggggcat tgcctgcttt gttaacgcct gcatcgacac aggcagtttt gaaacccagt
     4081 tgtgcggcac ggatggcggc aacgtatccg cccggacctg caccaatcac tactacatca
     4141 tattgagaca tcgtgtcatc ctttgctttt agcagtatct caaaataaaa acgatacttt
     4201 tcgtttcttt ttttattttg aatgactgcg ttttgatggt tttaagaaaa agccgtctga
     4261 aagtccatat ctgccgattc attcgaatca aatgtttcag acggcctcaa tcgttcgttg
     4321 agcctattgt agcacgatac tacattacgc tacatctaaa taaaaattca gacggccttt
     4381 tcattaacaa ataaaaagcc gtctgaaacg attacagatc caacaacagg cggaccgggt
     4441 cttccaacgc gtctttaatg gctaccaagg tcaatacagc ttcgcggccg tcaatgatac
     4501 ggtggtcgta agacagagcc agatacatca ttggacggac aacaacttgg ccgttttcaa
     4561 ccacagcgcg ctctttagtg gcatgcatac ccaaaatcgc agattgaggc gggttgatga
     4621 tcggggtgga catcatagaa ccgaaagtac cgccgttggt aatactgaat gtaccgccgg
     4681 tcaggtcttc gatagcgatt ttgccgtctt tggctttttt cgcgtaatca acaattgctt
     4741 gttcgatgtc ggcaatgctc atttggtcgg catcgcgcag gattggtaca accaaaccgc
     4801 gtgggctgcc aattgcgata ccgatgtcga agtagccgtg gtacacaatg tctttgccgt
     4861 caacagaagc attcacaacc gggtattttt tcagggcggc aacagcggct ttaacgaaga
     4921 aggacataaa gcccagtttt acgccgtgtt ctttctcgaa tttttctttg tacttcgcac
     4981 gcaagtccat gatcggtttc atgttgactt cgttgaatgt agtcagaatg gcgttttctt
     5041 gttgagaagc caagaggcgt tctgcaacac gggcacgcag gcggctcatt ggtacgcgtt
     5101 cttcaggacg tgcgccggca ggaagtgcaa cggcaggggc cacggctgcg gcaggtttgg
     5161 cagcggcatt ttgtacgtct tctttcaata cgcgaccgtc acggccggaa ccttgcaata
     5221 cgttcacgtc aacaccggtc tcggcagcca gttttgcagc ggcaggcatg gcggcgttgt
     5281 tttgtgcagc agcaggaacg gcagccgggg cagcttcggc aggagcggct gcaggtgctt
     5341 cagcagcgac agtagcagct gtatcgatac gggccaaaac ttggtcggca acaacggttt
     5401 caccgtcttg cgctacgatt tcaaccaata cgccggcttg tggagaaggt acttccaaaa
     5461 ccactttgtc cgtttcgata tcgatcagga tttcgtcacg ggcaactgct tcgccaactt
     5521 ttttcttcca ttccaagagt gtgccttcag atacgctttc agacagcata ggtactttta
     5581 catcaataat cattttgtgt ctccaatggc cctttcagac ggcctgttgt gtgtttttaa
     5641 ttctgcatat gggcattccg cctgagcaga ctcagacgga atgctacttg gtttataacg
     5701 ccaaagcgtc ttcaaccaat tgtttcaatt gagcaatgtg tttgcttgag tagcccactg
     5761 caggcgatgc gctgcttgga cggccgacat aagacagttt ttgctcttcg ctgataacgt
     5821 cttcgatgcg gtggcggatt tggtagaacg cgccttggtt tttcggctct tcttgcgccc
     5881 aaaccacaga ttttgcgttc ggatatttcg ccagttcggc tttaacctcg tcgtatggga
     5941 acggatacag ctgctcaacg cggacgatag cgacatcatc ttccagttta cgttcggctc
     6001 gaccggcttc caagtcatag taaacctgac cggcacacaa taccacgcgt ttcacgctgt
     6061 cgttgcttgc gcgttcggcg gtatcaccaa taaccggacg gaaagtcgaa ccttcggtga
     6121 agttttccag cgggctcatt gcacctttga agcgcaacag gcgtttggac atgaaaatca
     6181 ccagcggttt gcggtatgaa cccaagactt ggcgttgcaa gagatggaac atttgcgacg
     6241 cttcagacgg catgatgact tgcatattgt tctcagaaca cagttgcaac caacgttcta
     6301 cgcgtgcaga agagtgttca gggccttgac cgtcgtagcc gtgcggcagg atggtggtca
     6361 gaccgcacaa gcgaccccac ttggtttcgc ctgaagacag gaattgatca atagttactt
     6421 gcgcgccgtt ggcgaagtca ccgaattgag cttcccaaat ggtcagcttg tcaggagcgg
     6481 agcaggcaaa gccgtactcg aacgccatca cggcttcttc gttcaagata gagtcgataa
     6541 ccaggaactc gcccaagcct tcgcccatgt tgcgaagagg aacataagta ccgtcgtccc
     6601 atttttcgcg tttttgatcg tgcagaacgg catgacggtg tgagaatgta ccgcggccgg
     6661 agtcctcacc ggagatacgc acgccatgac ctttggtcag caggcttgcg tatgcgaggg
     6721 tttcggccat accccagtcg atggcttgtt tgccggatgc catggcttta cgcgcttcga
     6781 tcacacgttt tgcagtcgga tgcagggcaa agccttccgg cacagcggtg aatttctcag
     6841 taagacgctc aatgtcggcg gcaggcaaac cggtttcgat tttttcgcgc caatctttac
     6901 cttggtattt gctccagtcg atttgtgtgc gttggaagtt gctcaacgtt gtttgctcaa
     6961 catgttcgcc tttgtccaaa gcatcacggt aggcttggat gtaaccgtcg gcctcaactt
     7021 gagttaccac gccttcggca atcagttgct cggtgtacaa agcacgcgca cccggatgtt
     7081 gcgatacttt tttgtacatc atcggttggg tcaaagtcgg atcatcgccc tcgttgtgac
     7141 cccatttacg gtagcagaca acgtcgatta cgatgtcttt atggaatttt ttgcggtaat
     7201 ccaaagcggc ttgaatggca aagcaaacgc gttcgggatc atcgccgttc acatggataa
     7261 ccggagcgga aaccattttt gcgatatcgg tacagtgtac ggttgaacgg gtatcgcgga
     7321 tatcggaagt ggtaaagccg atttggttgt tgatgacgat atggaccgta ccgcctgtgg
     7381 tataaccgcg cgttttagac aggttgaatg ttgcttggtt gacacccaaa ccgataaatg
     7441 cggaatcgcc gtgaatcaat accggcaaga ctttatcgcg gccgttttcg cccaaacgtt
     7501 tttgtttggc gcgtgcagaa ccttccacta ccggattgac gatttccaag tgtgacgggt
     7561 tgaacgccaa agaaacgtgc atcgggccgt gaggggtggc aatatcggag ctgaagccca
     7621 tatggtattt cacgtcgcca ctaggcagtt tgatttcggc acggccttca aattcggcaa
     7681 acaaatcgcc gggttttttg cccaaaatgt tcaccaaaac attcagacgg ccacggtgcg
     7741 ccataccgat gatgacttct tccacgccgt ccttaccggc gttttgaatc aggtagttca
     7801 aaccggcaat cgcgctttcg ccgccttcga caccgaaacg tttctgaccg acatatttgg
     7861 tatgcagata acgttccaaa gtctcggcag cggtcatctc tttcaagata cggcgttttt
     7921 gatcggcatt gtaatgcggt gtggacaata cgctttcaaa atagttgcgt acccaacggc
     7981 gctcttcggt attgggaata tagatatatt ccaatgcgat gtggccgcag taggtttgtt
     8041 tgaggttgct gatgatttgg gacaagggca gtttgccgcg attggcaaaa tcaccctcgc
     8101 ccatattgaa tcgaagcgcc atatcggcat ctgacagacc gtggaatttc ggatcgaggg
     8161 cttcaatatc gcgcggaggg atacgtttga gcggatcaag ttgggccgca cccacgcctt
     8221 ggatacgata ggcagaaatc agccgtaaaa cgctgacttg ctttttcagc attgcctcat
     8281 ccgcaccgcc cgcaacggca gatgcaattt tctttttcgc caaagtaaca aatgattcgc
     8341 gaatcggtgt gtgtgcgaca tcgacagcaa ccgtccccgg ctgtttgctc aaatcggtga
     8401 aatactgctt ccatttttca tcaaccgcat cggggttttc caaaaaagcc tcgtacaatt
     8461 cctcaatgta gggtgcgttc gaaccgaaca gataagagaa attgagtttt tcgtccatca
     8521 tggcgtgcgc tcttttcttt aaaatataaa caaagggtag aagtccgcgt cctgacggct
     8581 gaactgatgc cgtctgaaaa gacattttat tctaccctat tttaaaccgg attgcttgtc
     8641 ttgatttcat ccgactacaa tcagacggat tcaggaaaca atccggttct ctgatattgc
     8701 ctgtttgaca atatattaat gtatgttacc tctcgcctgc cggcacataa tcgcggcgtt
     8761 ccgaaccggt ataaagctgg cgcgggcggc cgattttcag cgaaggatcg ctaatcatct
     8821 cgtgccagtg cgaaatccag cccacgctgc gcgacagggc gaagatgacg gtaaacattt
     8881 cggtcgggat gcccagcgcg gacaggacga tgccggaata gaaatcgaca ttgggataca
     8941 gtttgcgttc gataaagaac gggtctttca gcgcaatctg ttccaattcc atcgccagtt
     9001 tgaatttcgg actgtcttcc aagcccaatt ccttcaaaac ttcatagcag gtttcgcgca
     9061 taatgctggc acgcggatcc atattgcggt atacccggtg tccgaagccc atcaggcggt
     9121 atttgcgctg tttcacgcct tccatgtatg cggcaacatt ggacacatcg ccgatttcat
     9181 ccagcatttt caacacggct tcgttcgcac cgccgtgcga agcaccccac aggcaggcga
     9241 taccggcagc aatacaggca aacgggttcg cacccgaaga gcctgccaga cggacggttg
     9301 aagttgaggc gttttgctcg tggtcggcat gcaaaataaa gatgcggtcg agcgcgcgtg
     9361 ccaaaacggg attaggtttg tagtcttcac acggtgtggc aaacatcata tgaaggaagt
     9421 tttctgaata agaaagatta ttcttcggat aattgaacgg cagaccgttt gaatagcggt
     9481 aacacattgc cgcaatggtc gggattttgg aaataaggcg gtaaatcgcg attttgcggt
     9541 gttcgggatt agtaatgtcc aagctgtctt ggtagaacgc agacagcgcg ccgaccacgc
     9601 cgaccatcat cgccatcgga tgcgcgtcgc ggcggaaacc ccggaagaac caagtcagct
     9661 gttcatgcac catcgtgtgg cggcggactg tattgtcaaa ttctgccttt tgctcgggag
     9721 tcggcagttc gccgtaaatc aacaggtagc agacttccaa ataatcggac ttttcggcca
     9781 gctgctcgat ggggtatccg cgataataaa gcaagccttg atcgccgtcg atgtaagtaa
     9841 ttttagactc acagcttgcg gttgaaacaa atccggggtc gaaggcaaac aaacctgtat
     9901 tttttgtcag cccccgaatg tcaaccacat catgcccgat gctggcttcc aataccggca
     9961 gctccaaacc tgcccgaccc ggtacgttga gtttgattga tttggacata ttatcgctcc
    10021 tttgcaagtt ataacccacg ttactgcatc cgcagcacgt tctttgcttt cagacggcct
    10081 ttgcattttg aaatctgccc tcagacaaat ttcagatgcc tcatgcccgt ctgatttttt
    10141 caagcatcgg gataaggtgc cctttgtccg tttccgaatg cccgtgaatc aaggcaagca
    10201 attcttgatc ttgaaattca aggatttcgg aaaactcgga cagctcttta tcgctcaaat
    10261 gctcgaattc tttttccata aacctgccga agattaaatc tagttccaac aatccccggc
    10321 gggtttgaaa acggattttc cgtttggcaa tgtcgtcaaa aaccatcatt tcttaaacaa
    10381 cccgtttcaa cataatctct ttaatcttac cgatggcccg ggtcggattc aagtgtttag
    10441 gacatacgtc cacgcagttc ataatggtgt ggcagcggaa caaacggtac ggatcgttca
    10501 aattatccaa acgctcatta gtgatggtat ctcggctgtc cgcaatgaag cggtaggcgt
    10561 tcagcaagcc ggacggaccg acgaatttgt cgggattcca ccagaatgac ggacaggcgg
    10621 tcgaacagca ggcgcacaaa atacattcgt acaaaccatc caactctttg cgctcttcct
    10681 gagtttgcag acgctctttg tccgcatcaa tcggattgtc gttaacaaca taaggtttga
    10741 cggaatggta ttgtttgaag aactgggtca tatccacaat caggtcgcgg atgacgggca
    10801 gaccgggcag cgggcggatt ttgacaggct gtttcaagct gcgtaaatcg gtcagacacg
    10861 ccaagccgtt tttgccgttg atgttcatac cgtccgatcc gcaaatccct tcgcggcagg
    10921 agcggcggaa agacaaggta tcgtcttgtg ctttcaggcg taccaaagcg tccaaaagtt
    10981 tcacgtcggt cggttccaat tccaactcgt aacgctgcat ataaggcttg gcatcaacgt
    11041 ccgggttgta acggtaaatt tcaaaactca ttttttccat gagaagtgtt ccttttctca
    11101 tcaactgccc aatgattttg tattataaat tcaagcagtt caatgtgggt atggttaggt
    11161 ttcagacgac ctttgaggcg aagaccgtct gaaaacgcat caataaacgc gtttggccgg
    11221 tttgatgtat tccacgctca aaggcttggt gtgtaccggt ttgtaagaca aggtattggt
    11281 gtccgaatga tacagggtgt gtttcatcca attttcgtca tcgcgctcag gatggtcgtc
    11341 tgaagcgtgc gcaccgcgtg attctttacg tgcttcggca gacaccaaag tcgctttcgc
    11401 cacttcaatc aggttatcca attccaaagc ttcgatacgc gcggtattcc acactttgct
    11461 cttgtctttg atttcggtac gtttcacacg ctcggcaatc gccatgattt cttgaacgcc
    11521 tttgctcaga atctcatcgg tacggaacac gccggcgtgc agttgtacgg agcgttgcag
    11581 ttcgcgacgc aatgcatcaa cgttttcacc atcggtttga ctgtccaaac gctcgatacg
    11641 ttggcgggtc aactcaccgg cattagcagg cagaggtttc caatcgcttt gctctttgat
    11701 gaatttaatc atgctgtcac cggcagcttt gccgaacacc accaagtcca gcagggagtt
    11761 cgtacccaaa cggttcgcac cgtgtacgga agcacaggcg cactcacctg cggcatacag
    11821 gccttttaca ggtacttcgt actcgtcgcc ttgcggaaca acaacttcac cgtgataatt
    11881 ggtcggaatg ccgcccatca tatagtgggt agtcggcaca accggaatcg ggtctttaat
    11941 cggatcgata ccggcaaact gaatggaaat ctcgcggatg cccggcagtt tttccataat
    12001 tttttctgca ccgatatggt cgattttcag taagacgtgg tctttgtttt taccacagcc
    12061 gcgaccttca tagatttcca tcgccatcgc gcgtgaaacc acgtcgcgag aagccaagtc
    12121 ttttacggtc ggcgcatagc gttccataaa gcgttcgccg tcggcgttca acagaatacc
    12181 gccctcgccg cgtacgcctt cggtaatcaa cacgcccgca cccgccacgc cggtcgggtg
    12241 gaattgccag aattccatat cttccaacgg aatgcccgca cgggcgcaaa tgcccaaacc
    12301 gtcaccggta ttcatataag cattggtaga agaagcataa atacgaccgc cgccaccggt
    12361 agcaaacatc acggccttgg cgtggaaaat ataaacttcg cccgtttcca tttccatggc
    12421 ggttacgccg acgacatcgc cgttttcatc acgaatcaaa tcttgcgccg tccattccac
    12481 aaagaattgt gtattggcac ggacgttttg ttggtacaaa gtatgcaaca tcgcatgacc
    12541 ggtacggtcg gcaaccgcac atgcacgttc taccgcacgt ttaccatgtt cggcagtatg
    12601 tccgccgaaa ggacgctgat aaattttgcc gctttcaacg cggtcaaaag gcatacccat
    12661 gtgttccaac tcaatcaccg cttcaggcgc agcgcgacac ataaactcaa tcgcatcttg
    12721 gtcgcccagc cagtcggaac ctttcacggt atcgtacatg tgccagtccc aacggtcctc
    12781 ctgcacatta cccagagagg cggaaatacc gccctgcgcc gctacggtat gcgagcgggt
    12841 cgggaacact ttagacaaaa cggcacaatt caaaccggat ttggataatt ggagggctgc
    12901 acgtaaacct gcaccgccac cgccgacaat cacggcatca aacttgcgaa caggaaaacc
    12961 catacttacc cccaaatcac tttaactgaa tacacgaggc agccgaccag ccagacaatg
    13021 gtggcaacct gcaaaaacaa acgcacgccg aagggtttga tatagtccat ccacaaatcg
    13081 cggataccca cccaagcgtg caagaatacg gcgataaagc tcacttgggt aaatactttt
    13141 acccaagctt gactaaaaaa tgcctgccat gccggatatt ctttaggcag ggcaaataga
    13201 accactaaaa gtgcaacggt ataaatcaac ataataaccg cagtcgcacg ctgcattacc
    13261 caatcgcgca aaccgtaatg ggcaccggtc aatttacgtt ctaccataac aacgctccca
    13321 aaacgacagt caaaaccaat gcggaagcaa atacggcttt agcggtattg cgcgcagtat
    13381 tcagctcaag gcctttgtgc gcatccaaaa ataaaaagcg gataccggcg agagaatggt
    13441 gcagataagc ccacaatata ccgattaaaa ccagcttgac caaaggatgg gaaacaatgg
    13501 cacggtaagt ttcaaatgca gactcttgac tcagggtacc ggacagaaaa tacagcagga
    13561 aaggcagcat aataaacagc ccgaccccgc taatgcggtg aaggatggaa actatcccgg
    13621 gtatcggcag acggatgttc ggcaaatcca gataaacagg acgcagtttg acagacatag
    13681 tcaattcctc tgtagttata tatcgactca aaaaccgtaa acccattgct acactgggtt
    13741 acatcaacgt taatttacct gttttatccc attttgaaca gtgtatttta aaaacaaact
    13801 gatagatttt gacaaacacg cgtggaaaca gcagattaaa agaaagattt ttagttttgc
    13861 cgctcccttc ccgacacggt atcagcatcc gacccccaac cccatcccca aaaataaaat
    13921 gccgtctgaa aaaaatttca ggcggcattg ttttgccata tcggtcaggc tttcaatttc
    13981 gcatcggcat cgcgcaacgc ggtacgcaga ccttcctcga taacaggatg gtagaacggc
    14041 atatccagca tttgcggaac ggtcatcttc atttgatgcg cccatgccaa caagtgagcc
    14101 aaatgttcgg cggcagggcc tacgatttcc gcgccgataa agcggccggt ggctttttcg
    14161 gcatacaggc gcatatggcc tttgtttacc agcatcacgc ggctgcgacc ttgatttttg
    14221 aacgatactt cgccgatgac aaattcgtcg gcttggtatt gcgcggcaac ctgcgcgtat
    14281 ttcaaaccga caaagccgat ttgcggactg gtaaacacca cgccaatggt gctgcggcgc
    14341 aaaccgctgc cgatattcgg gtagcggccc gcgttatcgc cggcaatctt accttggtcg
    14401 gcggcttcat gcagcagggg cagttggttg gacgcgtcgc ccgcaataaa gatatgcgga
    14461 atgctggtct gcatggtcag cggatcggca acgggtacgc cgcgcgcgtc tttgtcgata
    14521 ttgatgtttt ccaaaccgat attgtcaacg ttcggacggc gacctacggc tgccaacata
    14581 tattcggcaa caaatacgcc tttttcgcca tcctgctccc aatggacttc tacattgccg
    14641 tctgcgtcga gtttgacctc ggttttagca tccagatgca gtttcaattc ttctccgaac
    14701 acggctttcg cctcgtctga aacaacgggg tcggaaatgc cgccgatgat tccgcccaaa
    14761 ccgaaaattt caactttcac acccaaacgg tgcaatgcct gacccagttc caaaccgata
    14821 acgcccggcc cgaacacggc aacgcgctta ggcagcgtat cccacgagaa aacgtcgtcg
    14881 ttgataatca aacggtcgcc caaagactgc cactgcggca ggatgacggg acgcgaaccg
    14941 gtagcaatca cgaaactttt tgccgcaatt tggatatggt cgtcgatttg gacggtatgc
    15001 tcgtcgatga atttagccga gcccatgatg cgcttgtcgg caggccattc ttccacatcg
    15061 gtaacgacaa agccgacaaa acggtcgcgc tcggatttga cgcgccgcat aacctcttct
    15121 ccgttgacga cgatgctgtc tttgtccaaa tgcacgccga aggggtcggt atgcaatgcg
    15181 tggtgacgcg cttctgcggc ggcaatcaag agtttggaag gcatacagcc cacgcgcgcg
    15241 caggttgtgc cgaacacatt gttttcaatc aggtaaacat tatccgaatg taaacgggca
    15301 ttgcgaaacg cacccatacc ggcagtaccg ccgccgatta cgacgatatc cgcttgaatt
    15361 tttttcataa tctttgtcct tccgttttcg tctgaaacat tgccgacaca gaacatatcg
    15421 gtttcagaca gcatttaaat atcttcccaa tcaaaaacat tattcagata aataaaaaca
    15481 aaatttcgat tgagtgcgac aatatccaaa tcttcttgta aatatagagt tattcggagc
    15541 ggcagtatag aaccgcccga ataactctat ccggtatatc agacagttta atcaaactgc
    15601 cttaagcaaa cagctatcag tttttagcca agtaagcttc caaatcttcg ctgccgccga
    15661 tgtatttgcc gccgatgaag acttgagggg cagtcatctt gccggtaata gcgcgaacgg
    15721 aagtaacggt tgcatctttg cccaatacga tttcttcgta agacaaacct ttgtcttgca
    15781 aagcttgttt gactttggca cagaattggc aacctggttt agtgaaaatt gccacagact
    15841 cttgagcctt ccaatcggga gcaacgaatt tcagcatagt atctgcatcg gatactttga
    15901 aaggatcgcc cggttcttca ggttcgatga acattttttc aaccacgccg tcgttaacca
    15961 gcatggagta acgccaagag cgtttgccga agcccaagtc ttctttaccg accagcatac
    16021 ccataccttc ggtaaattcg ccgttgccgt caggaatcat gtagatgttg tctgattctt
    16081 cttcggcagc ccaagcgttc attacgaaag tatcgtttac agatacgcag cagattgcgt
    16141 caacgccgtt ttctttgaac gcgccgaata attcgttgta acgcggcagg tgtgaagaag
    16201 aacaagtcgg ggtaaatgca ccgggcaggg agaatacgac tacttttttg cctttgaaca
    16261 aatcatcggt agacacatct ttccaagtgt cgccgacgcg ggtgcggaat actacggaag
    16321 gtactttttg accggtacga tcttgcaaag ccattttgag ctccttaata gattggtttg
    16381 agaaataatt gtgaatcgcc gcgcattcta ccccatgcaa aatgatttgt ataatttata
    16441 gtttaaataa tataaattat ttaaaactat caaatacaac tccgatttgc ctgttcccca
    16501 catataaagc cgaattagag aaaaacaagc tgcaatgaaa agattttccg gcaactgctc
    16561 ccctgcgccg caactcagga caaaatgccg tctgaaaggc tttcagacgg catttgttca
    16621 tacggtctcg gcttatttcg ccaattcggc acgcagtttg cgggttacgt tcatcattac
    16681 ttgaagctgt tccagagttt ctttccagcc gcgtgttttc aggccgcagt ccgggttaac
    16741 ccacagacgc tcaaccggaa caacctcaat ggctttgcgc aacaggtgtt ccacttcggc
    16801 ttctgtcggt acgcgcgggc tgtggatgtc gtaaacgccc gggccgatgt cgttcgggta
    16861 tttgaattcg ccgaacgcag ccaagagttc catgtcggaa cgtgaagtct caatggtgat
    16921 cacgtccgca tccattgcag caatcgcagg caggatgtcg ttgaactcag agtagcacat
    16981 atgagtgtgg atttgagtgc tgtcttcgca acctgcagag gatagacgga aggattcgcc
    17041 cgcccagttc aggtaggcat cccaatcggc acgtttcaaa ggaagacctt cgcggatggc
    17101 aggttcgtca atttggatga ctttaatgcc ggctttttcc agatccaata cttcgtcgtt
    17161 cagagccagt gcgatttgtt tgcacacggt agcgcgcgga atatcgttgc ggacgaaaga
    17221 ccattgcaga atggttacag gaccggtcaa catacctttc atcgggcgtt tggtcaggtt
    17281 ttgtgcgtaa gtagaccaag cgacagtcat agcttcagga cggcttacgt caccgaagat
    17341 gataggtggt ttaacgcagc gtgagccgta gctttgcacc cagccgtatt gggtgaatgc
    17401 aaaaccgctc aacagttcgc cgaagtattc gaccatgtcg ttacgctcgg cttcgccgtg
    17461 caccagtacg tccaagtcca gtttttcttg ctcttcaacc accaaggcga tttctttttt
    17521 cattgcggct tcgtaatcgg aggcagacag ttcgcctttt ttgaaggctg cgcgtgcttg
    17581 gcggatttcg gtagtttgcg ggaaagaacc aatgttggtc gtcggcagca gaggcaggtt
    17641 caaccatgct tgttgcgctt tgatacggtc ggcaaacggt gatttgcgtt ggtctgcgtt
    17701 gacaggcagg tcggccaagc gtttggcaac gtctgcacgg tggatttcac tgctgttggc
    17761 acgggagtcg gcagcagctt ggctggcggc cagttcttcg gcaacagaat cacggccttc
    17821 attcaatgcg gctttcagaa cacgcaattc ttgggttttt tgcagagtga atgccaacca
    17881 agagtacagg tcgggtttgt tggctttcag tttttcttcg actgacaagt caaacggagt
    17941 gtgcagcagc gagcaagagc tggaaatcca caaacgctcg cccagtttgg cttgcagagg
    18001 cccgacggtt tccaaaactt tgttcaggtt ggcgcgccaa atgttgcggc cgtcaatcac
    18061 gccggcagac aggactttgt cgtagccggc gaacgcgtcc agttgctcgg gtgcgcgtac
    18121 caagtcgatg tgcaggccgt caacaggcag ggatttcaac aatgcggcgt gttcggcaac
    18181 agaaccgaag taagtgccca acaggatttt ggcgcttact ttgttcaaag tagcgtaaac
    18241 gtctttgtat gcttctaccc attctttagg cagatcgaca gccaaagcag gctcgtcgat
    18301 ttgaatccac tcggcaccgg cttcaaccaa agcagtcagg atttcaacgt aaacaggcaa
    18361 cagtttaggc aacaggctca gacggtcgaa ttcaacggag cctttttctt tacccaccca
    18421 caggaaagtc aaaggaccga caacggtcgg tttggctttc aagcccaaag tttgggcttc
    18481 ttgcagttgt tgaacgtagt gtttggcgtt ggctttgaat tcggtatcgg catggaattc
    18541 aggcaccagg tagtggtagt tggtgtcgaa ccatttggtc atttcgatgg cgaattggtc
    18601 tttgttgccg cgcgccagtt ggaagaattg ttccaaaggc aggttttggc tatcgaagcc
    18661 gaaacgggcg ggaatcgcgc cggtggcaac ttgcaggtcg aggatgtgat cgtagaaagt
    18721 gaaatcacct acagcaacgt aatcagcgtt ggcagcagct tggtgtttcc agtttttctc
    18781 gcgcaggtct ttggcaacgt cgagcagctc ttgttcactg atctctttgc gccagtattt
    18841 ttcttgggcg aatttcaatt cacggaaggc gccgacacgc gggaagcctg agaaatgtaa
    18901 tgttgtcatg ttgaactctc cagttggatt ttgtaaggac cggttcaatc cgaatcggcc
    18961 ggtattgtaa atcagaatgg ctcggatagg gcggcgtttt gcttttcaga cggcctcgga
    19021 tgctgtctga agccgttttc ggctttaagg gcgcgcgcct aaaatatggc agatggcgta
    19081 agtcagctcg ctgcggttga gcgtgtagaa gtggaaatct ttcacgcctt cgcgggacag
    19141 gactttaacc atatcgatgg cgatactggc ggcgacgagg ttgcgcgtgc cttggtcgtc
    19201 gtccaagcct tcatacattt gcgacagcca tttcgggatt ttgacgttgg ttacttgtgc
    19261 cattttgccg agttgcttga agttggtaac aggcaggatg ccgggaacga tttcgacgtc
    19321 gatgcccaac atcacgcagc ggtcgcggaa gcgcaggtag cgttccacat cgaagaagaa
    19381 ttgggtgatg acgtggttcg cgcccgcatc gattttgcgc ttcagattaa tcaggtcggc
    19441 ttgtgcggat ttcgcttcgg gatgtacttc ggggtatgct gctacagaga tgtcgaagtc
    19501 ggcgacggag cgtaatagct taaccaagtc ttcggcgtaa aacggttttt tctcataacc
    19561 ggccggctcg tcgccacgca gggcaacgat gcggcggata ccgctgtccc aatagtcttt
    19621 ggcgatttgg cgcaattcgt cgtgggatgc atcgatgccg gtcaggtgcg gagctgcttc
    19681 caaaccggtt tcctgtttga tgcgtttgac gatgccgtgc gtgcggtcgc gttcgccgga
    19741 gtttgcgccg taagttacgg atacgaactt gggatgcagg gtttgcagac ggtggatgga
    19801 atcccacagc atcgtttcca tttgttcgtt tttcggcggg aaaaattcaa acgaaacgtt
    19861 gatgttgcct ttcaaatcgg aaaggctgtt gtttaacgca ttgatttctt ttgcataatt
    19921 ctgcatacct tcgttatctt ttacatgatg tttcaggcgg tatgataagg ttgcaccgaa
    19981 tatgagtcaa tttcaaaatt ttcaggttta tgatgaaaat ttttaatatt tagcaaaaac
    20041 tatacgcttt tcttgtacaa tccttctaaa ttatgataaa gtttgttatg ttataacact
    20101 tcaatcaagg caatatatga aaccgaatat tcatcctgac aattatcgaa ccgtcctttt
    20161 tttcgacagc agtgccaatg aaggctggct gattcgttct tgcgccggaa cgcacggaaa
    20221 aacaatggtt tggacagacg gcaaagaata tccgctcttt tccttagata cttcctcttc
    20281 ctcacatccc gtctataccg gcaaacagcg taacgtcaat accgaaggcc gtgccagcaa
    20341 gttcaaccaa cgtttccaat ccgtcatgtc atcgtttaga aaggacaaat aatgcaggtt
    20401 ctgtcttcgc tcaaaaccgc caaacaacgc caccgcgact gccaaatcgt cagacgcagg
    20461 ggcaaagtgt acgtcatctg caaaagcaat ccgcgtttta aatcgcgcca acgctgaccc
    20521 cctgcccttc ctgcttcggt tgtaaagccc ctcctaccgc aagccctttt caaatatgcc
    20581 gtctgaaaca gaaaaatgcg tttcagacgg cattttccgt cgatacgaat ccggaatgtg
    20641 tttgaaatac agcataaaca gataccaaaa cagcgtcgcg gcggctgcac cgagcagtac
    20701 ccatgcgctg atttcggcga cttcgcgcag gacacgtttc tgcctgcggc taaagaggcg
    20761 gttgataatc agcgcggcaa gtgcgaaaaa gaaaaaaata cggaaaagtc tgcctatcat
    20821 gtcgggaagc gaaaagtatg gttcgggcgc ggcggcatgg gaaaggatgt ttcagcgcgt
    20881 caggctgctt gtccgaccgt gtcaaaaagt catgccgttt gcgatataat aacgcttttc
    20941 gccgcatttg ttggaacagg atatgagtca aaatacctcg gtgggcattg taacgcccca
    21001 aaaaattccg tttgaaatgc cgctggtttt ggaaaacggt aaaactttgc cgcgtttcga
    21061 tctgatgatt gaaacctacg gcgagctgaa tgctgaaaaa aacaatgcgg ttttaatctg
    21121 ccacgcgctg tcgggcaacc atcacgttgc gggcaggcat tcggcggagg ataaatatac
    21181 gggctggtgg gacaatatgg tcggtcccgg aaaaccgatt gatacggaac gttttttcgt
    21241 ggtcgggttg aacaatctgg gcggctgcga cggcagcagc gggcctttgt cgatcaatcc
    21301 tgaaacgggc agggaatacg gcgcggattt tccgatggtt acggtgaagg actgggtaaa
    21361 atcacaagcc gcgcttgccg attatctcgg catcgaacaa tgggcggcgg ttgtcggcgg
    21421 cagcttgggc ggcatgcagg ctttgcagtg ggcgatttcc tatcccgaac gtgtgcgcca
    21481 cgccttggtg attgcgtctg cgccgaaact gtccacgcaa aatatcgcgt ttaatgatgt
    21541 agcacgtcag gcgattttga ccgaccccga tttcaatgaa ggacattacc gcagccacaa
    21601 caccgttccc gcgcgcggtt tgcggattgc ccgtatgatg ggacacatta cgtatcttgc
    21661 cgaagacggt ttgggcaaaa aattcggacg cgatttgcgt tccaacggct atcaatacgg
    21721 ctatagcgtt gaatttgaag tagaatccta tctccgctat caaggcgaca aattcgtcgg
    21781 gcggtttgat gctaatacat atttgctgat gaccaaagct ttggactatt tcgatccggc
    21841 ggcggatttc ggcaacagcc tgactcgcgc cgtgcaggat gtgcaggcaa aattctttgt
    21901 cgccagcttc agcaccgact ggcgtttcgc gcccgaacgt tcgcacgaac tggtcaaggc
    21961 actgattgcc gcccaaaaat ccgtgcagta tatcgaagtc aagtccgcac acgggcacga
    22021 tgccttttta atggaagacg aagcctatat gcgcgccgta acggcttata tgaacaatgt
    22081 tgacaaggat tgccgattat gaacctgcgc gatgatttgc aactgattta cgaccggata
    22141 cccgaaggca gccgcgtctt agacttgggc tgcggcgacg gcgaattgct ggcggctttg
    22201 gcggaacaca aaaaatgcag cggctacggc atcgaaatcg acaccgacaa cgtgattgct
    22261 gcgatgtcgc gcggcgtaaa tgttatccaa gccgatttgg aagaagggtt gaccgcattc
    22321 aacgatcaaa gttttgatgt gattgttttg agccaaacca tccaagcgat gcagaatacc
    22381 gaaaaaatcc tgcgctgcct gatgcgcctc gccaaacagg caatcgtcag tttcccgaat
    22441 ttcggctact ggcgcaaccg cgtccaaatc gcactcggcg gacatatgcc ggtttccgaa
    22501 cgtatgccct accattggta cgacacgccc aatatccatt ggtgcacgct caaagacttt
    22561 gatttgttgt gcgccaaaaa caatatccgc gtcctcgaac gcgccgtgat gacgggcaac
    22621 agacaggtca aacatttccc caaccttttg ggcagccttg cgttttaccg cgtcgggtaa
    22681 gacaggttga aaaccttgtc ggcaataacg ggtttctcgg ttctattaac aacagtccgt
    22741 ctgaaaaatg aaacggcagg ttttcagacg gccttttcaa tattggaaac aatagaatga
    22801 aaaacaaaac gtcatcactt cccttatggc ttgccgcaat catgctggcc gcgcgttccc
    22861 cgagcaaaga agataaaacg aaagaaaacg gcgcatccgc cgcttcgtct tccgcgtccc
    22921 aatccgattt gcaaccggcc gcatccgccc ctgataacgt caagcaggca gaaagcgcgc
    22981 cactgtgaaa ttgcaccggc ctgcaccccg ccgccggcat tggcgatctc atacagcaaa
    23041 tcgccgaaca catcgactcg gactgtctgt ttgccctttc ccataacgaa ctggaaaccc
    23101 gtttcggctt acccggcggc ggctatgaca acatacagcg gctgctgttt cccgacatcc
    23161 gccctgaaga tcccgactac catcagaaaa tcatgctggc aatcgaagac ttgcgttacg
    23221 gaacgcgcac catcagccgg caggcacaag atgccataat ggaacaggaa cgccgcctcc
    23281 gagaagcgac gctgatgctg acacagggca gtcaaaaaac ccgcggacaa ggcgaggaac
    23341 cgaaacgcgc acgttatttt gaagtttcgg caacatctgc ctatttgaac cggcacaaca
    23401 acggactcgg cggcaatttc caatacatcg gccaattgcc cggctatctg aaaatgcacg
    23461 gagaaatgct tgaaaaccaa tcactcttcc ggctgtccaa ccgtgaacgc aatcccgaca
    23521 aaccgttttt agacatccat tttgacgaaa atggcaaaat cacgcgtatt gtcgtttacg
    23581 aaaaaaatat ctatagtaga ttaaatttaa atcaggacaa ggcgacgaag ccgaagacag
    23641 tacagatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactatact
    23701 tcaatccaaa cacggggcga atataaaaaa atgccgtctg aaacgttttc agacggcatt
    23761 tttcaagtag gatatcaggc gcgtttgcgg aattcgccgg tacgggtatc gatttcgact
    23821 ttgtcgccgt tttcgatgta agacatcact tggatttcgg tgccgccgac cagacgcgcg
    23881 gttttcatca ctttgccgga agtatcgcct ttgacggcag gctcggtgta gtcgacttcg
    23941 cgcacgatga tggtgggcag ttcgacagaa atgggattgc cttcatagaa ggtaacttcg
    24001 cattggtctt ccataccgtc aacgatgaat ttcaacgcgt cgccgatgtt gtcggcttcg
    24061 atttcgtatt ggttgaattc ttcgtccata aagacgtaca tcgggtcggc aaaatagctg
    24121 tacgtacagt ttttgcggga caggatgacc acgtcgaatt tgtcgtcggc tttgtaaatg
    24181 gtttcggaag cagcgccggt cagcaggttt ttcagtttca tgctgacttt ggcggaagag
    24241 cggccgcctt tgatgtattc ggttttttga acgaccatag gatcgttgcc gaccataaat
    24301 acattgccgg cgcgcagttc ttgtgctgtt ttcattgtga ttttccaatc aaatcggttg
    24361 tcgtaaaacg cgctattcta gcgtattttt tgatgctttg aaacaaaggc ggcgagtttt
    24421 tcggatgcgg aaggctgccc gaaaagataa cggctccaat cctccgcgcc ttgccgccag
    24481 ccgttttgat gttgttgcag ggtttgccaa cattcgaggc gttgtgttgc ggataaagcc
    24541 tctccgccgt tgaggtcgtc cgaaaggagg cggtgcaccg atgcggtttc gggcgtgtag
    24601 aagccgtatg ccttatccca aaaggcgtgg agtttgtcga gatggacatt ctcgtcttgc
    24661 gggtagatgt gccaaaaaaa gggttttccg gcaagctggg tacgcacgaa actgtcttcg
    24721 ccgcgtatca cggcgcagtc ggcgaggtgc agcaatttgt cgaagtcctg ttgcggcacg
    24781 aacgggattt tgacaaggcg gacggatgcc gtctgaaaga caccgccgtc attttgcagg
    24841 gcgttttgcg gaataacgcc gctttgtttg aggctgtcga taatttgcgc ccccgccagc
    24901 agtagggtca tcaggctgcc tgcctgttgc cacatgtcca gccactttgc ccaaacatcg
    24961 ccccgatagc cgaaaagcag ccattcgggg gcgttttttt cgggcagcac cagccgccgg
    25021 cgcagggctt cggtatcgaa acggacggct tcgcggtaat cgcgttcgcg tatcaacccg
    25081 ccgctttttt cgctgaaacc cataaaccaa aaatattttt gaacgccctc ctgcggcgaa
    25141 ggcatcaggt gcagcctttc attgctttcc tccgcgctca aatattccca attcagccaa
    25201 agcggtttgt gtcggcggat gatgttcagc acattttcgg gcaggtcgca ggcaaaagtt
    25261 tcgataacgg caccgggaac gggcgcggta tcaatgtctg ccgcatcgga atgccaagtg
    25321 cggacatgaa tatcctgatg aacgaaggga acatcgggca aatcgggaca aagcgcgcgc
    25381 aaggcggaca cgtcgtccgt ccacaaatgc acctgccaac cgagttcgcg gtgcaaaaca
    25441 cgggcgagcc gccacgaaac gccgatgtcg ccgaaattgt cgatgacctt gcaaaaaatc
    25501 caacagacag gaaaagcgta tgtattcata accatcggga aaacaagcta aaacgcgccg
    25561 aaatagtgga acaaatacac aaccagcaaa acaatcggaa taaacagaaa catggtttga
    25621 cgtttcaaat caaacccttg tttaagatga tgcttgatgg tttcaaagag gacgaattta
    25681 aacaaaaacg tcgcttcgca ggcaaggatg atgccgttga tcagatgctg gggtttgtct
    25741 tcgggcggat tggcggcgaa aacggcaagc gtcgccaagg ggatgaggca gacggcgagc
    25801 cagcggacaa gcaggtttcg ggacattgca aggctttcaa aaaagcaaaa cgccattatc
    25861 gcctcaagaa cgcaccttgt ctaacggacg cgccggagat gccgtctgaa gccttgccgc
    25921 cggaaaacgc cgcaccgccg aacctttaaa tatacccggg acgggcgttc cccgagaaat
    25981 cggacgctgc cgccgatgcc tgcggacaag gcggcatcgg gcgcgggcat attgaaagcc
    26041 cgacgcatcg gtttgaacgc attttataaa ggcgtacggc aaacccgccc cttgccgtcg
    26101 gcgtgcttcc gcaaagcaaa atataagggc ggcgcgcttt ccgctataat ccgcattttt
    26161 ccaaacctga aacattatgc cgaccccgaa agcctttacc ctgctcgggc cgaccgcctg
    26221 cggcaaaacc gcgctcgccc tcaaaattgc cgaaaccctt ccggtcgaaa tcatcagcct
    26281 cgattctgca ctgctctata ccggtatgga catcggcacg gcgaaaccgt ccgcctccga
    26341 acgcgccttt gtcccgcacc accttatcga catcattacg cccgttcaga catacagtgc
    26401 cgcccgtttt gtcgaagact gcacgcgtct gaccggcgaa atcacggcgc gtgggaaatg
    26461 tccgctgatt gtcggcggca caatgatgta tttccgcgcc ctgacccaag gtttgaacga
    26521 tttgcccgaa gccgatgcct gcctgcgtgc cgacttggac gaacaaaaac agatgtacgg
    26581 tttggatttc ctctaccgca ccctgcaaaa agtcgaccct gaaacagcat gccgtctgaa
    26641 accgaacgac agccagcgca tcggacgcgc ccttgaaatc tattacctga ccggcaggcc
    26701 gatgagcgcg cacctgaacg gacagcccga acacaccctg cccttcgaac tctacaccgc
    26761 cgccctgatt cccgaagacc gcgcccgact gcacgaaaac atcgccctgc gtttccacct
    26821 gatgcttgaa cagggcttta tcggcgaagt agaaaacctg cgccgccgct atcccggcct
    26881 gaccgccgac tcccccgcca tccgctgcgt cggctaccgt caggcgtggg aacacctcga
    26941 cggtgcaacc gacagacaaa ccttcatcga aaaaggcatt gccgccacgc gccaacttgc
    27001 caaacgccaa ctaacttggt tacgcaaaac acctttagac tgcgttgccg acccgttttc
    27061 agacggcact tcaggcacgc gcctgattga ggcggcaaaa cggtttttcg gcgaataaac
    27121 catgctggcc acgccgccgc tcgcccccaa aaccgtcgcc gccctgcaca ggctcggtat
    27181 ccgtacgctc gaagaactgc ggcaaaacgg ttcggtcaaa gcctttctgc tgctcaaagc
    27241 atcgggtttg accctcacga aaagcacgct gtggcaactc gagtcgctgc tgaacggcac
    27301 gccgccgcaa gaaatgcccc aagcgcataa agactgcctg cttgccgagt tgaaaaacca
    27361 tccgcccgtc gccgcctttc cgccgcagga agaaatggga tacttcatgc gcgaagcact
    27421 gcgccaagca gaacaatccg ccgccgacgg cgaaataccc gtcggggcag tcatcgtttc
    27481 agacggcaaa atcatcgcat cggcacacaa cacctgcatt gccgactgca atgtcagccg
    27541 acacgccgaa atcaacgcct tggcacaggc aggcagcgaa atgcaaaact accgccttga
    27601 cggatgcgac atctacatca ccctcgaacc ctgcgccatg tgcgcgtccg cactgataca
    27661 ggcacgaatc aggcgcgtga tctacggtgc ggcagaaccc aaaaccggcg cggcaggcag
    27721 catcgtcaac ctgtttgccg acaaacgcct caatacgcac accgccatac ggggcggaat
    27781 cctgcaagaa gaatgccgcg ccgtattaag ccgttttttc caaaacaaaa gaaaaggttg
    27841 aacccatgac cgccctactc gtcatcctcg ccctcgccct gatagccgtc ggcacggcag
    27901 gcatcgtcta tcccgccctg cccggcttgg cattgatgtt tgccggaaca tggctgcttg
    27961 cctatgccgg cggctatcaa atctacggcg caggcatctt gtggacggtc ggactcatca
    28021 gccttggcgg catactggcg gactatatgg caggcatgtt gggggtaaaa tacactgggg
    28081 caggcaaact cgccgtccga ggtgcattgg ccggcagcat catcggcata tttttctccc
    28141 tgcccgggct gatactcggc ccctttatcg gcgcggcggc aggcgaactg atcgatcggc
    28201 gcaatatgct tcaggcaggt aaagcgggct tgggtacgct gttggggctt gtcgtcggca
    28261 cggcgttcaa aatcggctgc gccgtatcca tcttgtttat cctgttggtg aaatacatcg
    28321 cctacctgtt ttaacccctt cagacggcat accggcaaat tgctataatg ccgtctgaaa
    28381 tttccgacaa agcaccatta tggcaaacca aagacccatc tacggcttcc acgccgtcaa
    28441 cgcccgattg tggcaaaacc ccaaatcgat taccgaactc tatatccaag aaggcaaatc
    28501 cgacgcacgc acgcgcgaag tgctggaaaa ggcggcaaac gaaaacatcc gcgtatattt
    28561 tgccgatgcc gaccgcctca acgccatcag caaaggcgcg cgccatcagg gcgtggtcgg
    28621 atttatcgat gcctccaaaa accacgtcca cctcgaaggc atattggaaa acttgagcga
    28681 accgccgctg ctgctgatac tcgacggcat caccgatccg cacaacctcg gcgcgtgcct
    28741 gcgtaccgcc gacgcaatgg gcgtacacgc cgtcatcgcg ccgaaagaca aaagcgcggg
    28801 actgaacgcc accgtcagca aagtcgcctg cggcgcggcg gaaaccgtcc cctacatcac
    28861 cgtaaccaac ctcgcccgca ccctgcgcga attgaaagaa tacggcattt ggattatcgg
    28921 caccgacatg ggcggcgacg ccgaccttta ccattgcaat ctgcccgaca gcacggcgtg
    28981 ggtgatgggc aacgaaggcg acggtatgcg ccgcctcacg cgcgaacatt gcgacatgct
    29041 ggtgtccata cccatgttcg gcacggtcga aagcatgaac gtctccgtgt ccgcaggaat
    29101 ggtattaagc gaaacccgcc ggcaacgcgt attgaaaaac aaaaaagcgt aagcctcaaa
    29161 aacaatgccg tctgaaacag ggattctgtt tcagacggca ttcattttca aaatcagtgt
    29221 tccaaatggt cggtatcaaa cttgaccgcc gcctcggttt tatcttccgg caagaccaaa
    29281 ttcagcaaga ctgccgtaat gccgccgccg gaaatagagt tttggaacaa gacgggcagg
    29341 tttttaaaca cttccggctc aaaccccacg cccaagccca aaccgaccaa cgttgccgca
    29401 atcaccgccc cgcgcctgcg gatgccgtgg ccgaccaaaa tccgcacgcc cgcaatcgca
    29461 atcaggccga acatcaaaac catcgcgccg cccaacaccg gactcggaat cgtcgtaaac
    29521 gcgcgtccga caacggggaa cagacccaac agcaccaaaa tcacggcaat atatttgccc
    29581 acgtggcgcg aagccactcc ggtcatctga atcacgccgt tgttttgcgc aaacgtcgtc
    29641 agcggcagcg aacccaaagc cgtcgcaatc accgatacca agccgtcagc caacacgccg
    29701 ccgcgcaggc gtttggtgta ttcttcgcct tcaatcggct ggtcggacac cattgccgtt
    29761 gccgtcaaat cgccgaccgc ctcaaacacg cccaacaaga aaatcgcacc cgccgcaata
    29821 aatgcgtgcc agtcaaaagc aaaaccatat ttaaacggta cgggcagcgt aaccggcggc
    29881 aggttttgca gtgcggaaaa atccaccttg cccaaaaaca gcgcgacaat agtagccggc
    29941 aatcagaccg accgcaatgc cgctcatccg cagcagcgga tttttcatgc agttgaacac
    30001 cagcacaatc agcagcacca gcgaagccag ccccaagttt tccatcgagc cgaacgtgcc
    30061 gtccgccttc gcgccgaagc cgccgccgaa atcggcaata ccgacgtgta ccaaactcag
    30121 accaatcagc atcacgacca cgccgctgac cgtcggcgta atcacttttt tcaaatacgg
    30181 caaaagccac gccgagaaac acaccaaaaa cgcaccggca aacgatacgc ccaagagcgt
    30241 cgaaaccatc gccccctcac tcaaaccgcc ctctttcatc cccgcgccga gcgcaatcat
    30301 gacggtaacg ctggatggac agcatccccg agccgaccgg cccgaagcgg ttgacctgca
    30361 aataagtgcc gatgccggaa gccaccatcg ccatcgacac gagatacgcc gtcatttcca
    30421 ccggtagttc cagcgcgccg cccacaatca gcgcgggcgt aatcatcggc acgaaaatcg
    30481 ccaaaagatg ggtaaccgcg ctcaagagcg cattaccgaa cggtggcctg tcttccaaac
    30541 cgtacaccaa atcaggcgaa tccgcctgtt ttttcattgt ttcagccatt ggagtctttc
    30601 tatccgttgc caaacgttaa aaaattttaa gaacgaaatt gtaacgaatc aaagattcct
    30661 tatcaattca aatcctcatg tgcaaaaaca ccaccggttg tccgaattca aatttctttg
    30721 aatttccaca ccggacagtc cttcatttcc agagagcctt catgcgctta acacatttta
    30781 atcctcaacg gcgcatccac tgcttaaacc caataatttc atgattataa aaaaaatatt
    30841 atttattaag atatgatggg acttaagcct tggaaccaac cgtgcggccg atatgtttca
    30901 cacaaacaac aaagtaagct acaatccgaa gcataatcga cctcactcaa tgaataaaag
    30961 gtttccgtat gttacaaggt agcttggttg ccctgattac cccgatgaat caagacggca
    31021 gcatccatta cgaccaactc cgccaattaa tcgactggca catcgaaaac ggcacggacg
    31081 gcatcgtcgc tgtcggcacg acaggcgaat ccgccaccct ctccgtcgaa gaacatttaa
    31141 gcgtaatcga agaaacggtc aaacacgttg ccaaacgcgt ccccgtcatc gccggcacag
    31201 gggcgaacaa caccgtcgaa gccatcgccc tttcccaagc cgccgaaaaa gccggcgcgg
    31261 actacaccct ctccgtcgtc ccctattaca acaaaccttc ccaagaaggc atgtaccgcc
    31321 atttcaaagc cgttgccgaa gccgccgcgg ttccgatgat actctacaac gtgcccggca
    31381 gaaccgtcgt cagcatgaac aacgaaacca tcctgcgcct tgccgaaatc cccaacatcg
    31441 tcggcgttaa agaagcgagc ggcaacatcg gcagcaacat cgaactcatc aaccgtgcgc
    31501 ccgaaggctt cgccgtcctt tccggcgacg accacaccgc cctgccgttc atgctctgcg
    31561 gcggacacgg cgttattacc gtagccgcca acgccgcgcc caagctcttt gccgatatgt
    31621 gccgtgccgc gctgcaaggc gacattgccc ttgcacgcga actcaacgac cggctgattc
    31681 cgatttacga caccatgttc tgcgaaccca gtcccgccgc accgaaatgg gcggtatccg
    31741 ccttgggcag atgcggaccg cacgtccgcc tgccgctcgt tccgctgacg gaaggcggac
    31801 aggcaaaagt ccgcaccgcc ctgaaagcct caggacaact ttaaatatag ccatgccgtc
    31861 tgaaccgttc ggacggcata acatgacaaa cactttaata tccatcacac aggatgacac
    31921 gatgacccat atcaaacccg tcattgccgc gctcgcactc atcgggcttg ccgcctgctc
    31981 cggcagcaaa accgaacggc ccaagctcga ctaccaaagc cggtcgcacc gcctgatcaa
    32041 actcgaagtc ccgcctgatt tgaacaaccc cgaccaaggc aacctctacc gcctgcctgc
    32101 cggttcggga gccgtccgcg ccagcgattt ggaaaaacgc cgcacacccg ccgtccaaca
    32161 gccagccgat gccgaagtat tgaaaagcgt caaaggcgtc cgcctcgagc gcgacggcag
    32221 ccaacgctgg cttgtcgttg acggcaaatc ccccgccgaa atctggccgc ttctgaaagc
    32281 cttttggcag gaaaacggct tcgacatcga atccgaagaa cccgccatcg gacaaatgga
    32341 aaccgagtgg gcggaaaacc gtgccaaaat cccccaagac agcttgcgcc gcctattcga
    32401 cacagtcggt ttgggcggca tctactccac cggcgagcgc gacaaattca tcgtccgtat
    32461 cgaacagggc aaaaacggcg tttccgacat cttcttcgcc cacaaagcga tgaaagaagt
    32521 gtatggcgac aaaaacaaag acacgaccat gtggcagcct tccgcttccg accccaacct
    32581 tgaggccgct ttcctgacgc gctttatgca atatttgggc gttgacggac ggcaggcgga
    32641 aaacgcattg gcaaaaaaac cgacccttcc cgccgccaac gaaatggcgc gtatcgaagg
    32701 caaaagcctg attgtctttg gcgactacgg cagaaactgg cggcgcaccg gccttgccct
    32761 cgaccgcatc ggactgaccg tcgtcggtca aaacaccgaa cgccacgcct tcctggttca
    32821 aaaagccccg aacgaaagca atgcagttac cgaacaaaaa ccggggctgt tcaaacgcct
    32881 actgggcaaa ggcaaagcgg agaaacctgc cgaacagccg gaactgattg tctatgccga
    32941 gcctgtcgcc gacggttcgc gcatcgtcct gctcaacaaa gacggcagcg catatgccgg
    33001 caaagacgca tccgcactgt taggcaaact ccattccgaa ctgcgttaaa ccgttcaaaa
    33061 caaaagcagc ttccaaacag aagctgcttt tttgtgcgga actttttaca acaaacgggg
    33121 caaaatatcc tcaaccgcct gaaccaacgc atccgccaaa ggcggatcga acgcacaatg
    33181 ccccgcctgg accaccctca attccgcttc gggaaaggct ttcgacagct cccacgcact
    33241 ctgcataggc gtacacaaat cataccgccc ctgcacgata acggtcggga tatgccgtat
    33301 tttgccgata ttgttcaaaa tcgccttatc accctgcaac caaccgccgt tgacaaaata
    33361 atggttttcc aaacgcgcga ttgccagtga ggcataagca tcttcatcca ctccctccgg
    33421 ctcgaaacga atcagatagc tttcccaatc cgcccaagcc ttcgcggcag acaggcacac
    33481 ttcttcatct tgatgaaaca gcaacccgtg atacgcctca atcagccggc tccgccgatt
    33541 ttcagcaatc ggcgcgacaa atttttgcca ttgttccgga taaatccggc tcacgccgcc
    33601 cgcctcgttc agccacgccg tttcagacgg cctgcacaaa aatatcccgc gcaacaccaa
    33661 tccctttacc cgttcgggat gggtttgggc ataagccagc gacaaagtgc tgccccacga
    33721 accgccgaac accagccatt tcccgatacc cagcatttca cggacttttt caatatccgc
    33781 caccaaatcc caagtcgtat tgtcttccgc acaagcatac gggtgcgaac gtccgcaacc
    33841 gcgctggtcg atgatgacga tgcggaacac atcaggattg aaaaaacccc gacattcagg
    33901 cgaagccccc gcgcccggcc cgccgtgtaa aaaaatgacc ggcacaccgt cgggattgcc
    33961 cgactcttcc caataaattt gatgaatttc agacacctgc aagtatccgc tatgaaaagg
    34021 ctgttttatt tcatacatca tcttctccca tattgcttct gacaatccga acatcccgac
    34081 acgcgacatc ccgcacgaaa cacaagccgc cccgaaaggc aaatgccgaa aaatcgcgat
    34141 aaaaaccatc aatcggaaaa tattgcataa acaggcgaaa atatatcatt gaaaagaaaa
    34201 cccatacaat gtctgacaga aacaagccgc tttgccattg tttcctaaca gttaaacccc
    34261 gcccttcaag gcggcggggc agggcttgat tcaaaatggc gcaagcccct gccctcaaat
    34321 ccaacacgca ggattaaacc ataatagcgg ctttcttatt atttcttatt gaaacaccgc
    34381 ccggaacccg atataatccg cctttcaaca tcagtgaaaa tctttttttt aaccggtcaa
    34441 accg
//
LOCUS       DAXDZG010000016        33820 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXDZG010000016 DAXDZG010000000
VERSION     DAXDZG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33820
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.016"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(2..466)
                     /locus_tag="ACFB78_000662"
     CDS             complement(2..466)
                     /locus_tag="ACFB78_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268710.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="ACFB78_000663"
     CDS             complement(543..3380)
                     /locus_tag="ACFB78_000663"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC1268711.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVAEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="ACFB78_000664"
     CDS             complement(3485..4744)
                     /locus_tag="ACFB78_000664"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC1268712.1"
                     /translation="MKVLVLGAGIAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="ACFB78_000665"
     CDS             complement(4823..6214)
                     /locus_tag="ACFB78_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1268713.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(6676..7452)
                     /gene="lpxA"
                     /locus_tag="ACFB78_000666"
     CDS             complement(6676..7452)
                     /gene="lpxA"
                     /locus_tag="ACFB78_000666"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC1268714.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7495..8181)
                     /locus_tag="ACFB78_000667"
     CDS             complement(7495..8181)
                     /locus_tag="ACFB78_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC1268715.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFSFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQV
                     IAVCDEIDKL"
     gene            complement(8388..8837)
                     /gene="fabZ"
                     /locus_tag="ACFB78_000668"
     CDS             complement(8388..8837)
                     /gene="fabZ"
                     /locus_tag="ACFB78_000668"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC1268716.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8873..9916)
                     /gene="lpxD"
                     /locus_tag="ACFB78_000669"
     CDS             complement(8873..9916)
                     /gene="lpxD"
                     /locus_tag="ACFB78_000669"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC1268717.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9949..10449)
                     /locus_tag="ACFB78_000670"
     CDS             complement(9949..10449)
                     /locus_tag="ACFB78_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC1268718.1"
                     /translation="MIRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10515..12893)
                     /gene="bamA"
                     /locus_tag="ACFB78_000671"
     CDS             complement(10515..12893)
                     /gene="bamA"
                     /locus_tag="ACFB78_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC1268719.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12950..14290)
                     /gene="rseP"
                     /locus_tag="ACFB78_000672"
     CDS             complement(12950..14290)
                     /gene="rseP"
                     /locus_tag="ACFB78_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC1268720.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14325..15560)
                     /gene="ispC"
                     /locus_tag="ACFB78_000673"
     CDS             complement(14325..15560)
                     /gene="ispC"
                     /locus_tag="ACFB78_000673"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC1268721.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQILHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15565..16362)
                     /locus_tag="ACFB78_000674"
     CDS             complement(15565..16362)
                     /locus_tag="ACFB78_000674"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC1268722.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16365..17111)
                     /locus_tag="ACFB78_000675"
     CDS             complement(16365..17111)
                     /locus_tag="ACFB78_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC1268723.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17167..17724)
                     /gene="frr"
                     /locus_tag="ACFB78_000676"
     CDS             complement(17167..17724)
                     /gene="frr"
                     /locus_tag="ACFB78_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC1268724.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17866..18858)
                     /locus_tag="ACFB78_000677"
     CDS             complement(17866..18858)
                     /locus_tag="ACFB78_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1268725.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18863..19699)
                     /locus_tag="ACFB78_000678"
     CDS             complement(18863..19699)
                     /locus_tag="ACFB78_000678"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC1268726.1"
                     /translation="MNIIDAIINLANNPVVGVESHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYIVGVVDKKKNLKHLAMVYGIDYCADAECYLK
                     IKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPWFVFNYIYQR
                     NMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPARLRNAKLITY
                     HL"
     gene            complement(19808..20935)
                     /locus_tag="ACFB78_000679"
     CDS             complement(19808..20935)
                     /locus_tag="ACFB78_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC1268727.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(21005..21080)
                     /locus_tag="ACFB78_000680"
     tRNA            complement(21005..21080)
                     /locus_tag="ACFB78_000680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21045..21047),aa:Thr,seq:cgt)
     gene            21187..21666
                     /locus_tag="ACFB78_000681"
     CDS             21187..21666
                     /locus_tag="ACFB78_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268728.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SDGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            21761..22384
                     /gene="rsmG"
                     /locus_tag="ACFB78_000682"
     CDS             21761..22384
                     /gene="rsmG"
                     /locus_tag="ACFB78_000682"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC1268729.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            22484..23257
                     /locus_tag="ACFB78_000683"
     CDS             22484..23257
                     /locus_tag="ACFB78_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC1268730.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(24003..24587)
                     /gene="rnhB"
                     /locus_tag="ACFB78_000684"
     CDS             complement(24003..24587)
                     /gene="rnhB"
                     /locus_tag="ACFB78_000684"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC1268731.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(24655..26550)
                     /gene="mnmG"
                     /locus_tag="ACFB78_000685"
     CDS             complement(24655..26550)
                     /gene="mnmG"
                     /locus_tag="ACFB78_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC1268732.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSVGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(26723..28150)
                     /locus_tag="ACFB78_000686"
     CDS             complement(26723..28150)
                     /locus_tag="ACFB78_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1268733.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            28309..29301
                     /gene="pdxA"
                     /locus_tag="ACFB78_000687"
     CDS             28309..29301
                     /gene="pdxA"
                     /locus_tag="ACFB78_000687"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC1268734.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(29531..32290)
                     /locus_tag="ACFB78_000688"
     CDS             complement(29531..32290)
                     /locus_tag="ACFB78_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC1268735.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            32783..33775
                     /locus_tag="ACFB78_000689"
     CDS             32783..33775
                     /locus_tag="ACFB78_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC1268736.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT         6707 a         8636 c        10075 g         8402 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtccat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct tcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtc tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaaacgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcactgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgacaccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggccacggt ttcggcgcgt
     2941 acgtcgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccactccc acactttttc caagaatttt
     3061 tcgcgcccca agtcgtgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaatacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccaaaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gcgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc actttgtagt gtccggtaat ttcagcacag aatattcaat aagacaatat
     6301 gttcttttga aaaatacttt tggttttttc accgaagaca ggccggttca agctgcggaa
     6361 attgtttgca attatttaaa agcagaggcg gaggtcacaa tgaaatgtcc gaatggggat
     6421 gtggcgggcg gcagaaatca tcaatgctgc cgactgccat acttctgaaa tctatagtgg
     6481 tttaaattta aaccggtacg gcgttgcctc gccttgccgt acttcttgta ctgtctgcgg
     6541 cttcgtcgcc ttgtcctgat ttaaatttaa accactatac aaaatgatgc atcgatcaaa
     6601 caacataccg ctttaaaaaa aaccgatgcc gtctgaaacg ctttcggggt ttcagacggc
     6661 attgaaaggg tgcggtcagc ggatgatgcc gcgcgtcgat tgtgcgaaga agtcttgaaa
     6721 tacggcaagc tcggcttggg tttcggcgcg gcggagaatg tccgccttgg cttcttcaaa
     6781 cggaatgccg cgatggtaga gggttttgta tacgtctttg acggcggaaa tctgttctgc
     6841 ggtaaaaccg ttgcggcgca taccttcgct gtttaggccg gccggttcgg cgcggtagcc
     6901 cgatgccata aagtagggcg gcacgtcttt gtgtacgcct gcggcgaagg cggtcatggc
     6961 gtagtcgccg atgcggcaga attggaagac cagcgtgtag ccgcccaaaa cgacgtagtc
     7021 gccgacggta acgtgtccgg caagcgaggc gttgttggcg aaaatggtgt ggttgccgac
     7081 cacgcagtcg tgcgcgaggt ggcagtacgc cataatccag ttgtcgtcgc cgatacgggt
     7141 ttcgccgatg ccggttaccg tgcctaaatt aaaggtggtg aattcgcgga tggtgttgcc
     7201 gttgccgata atcagcttgg tcggctcgtc gcggtatttt ttgtcctgcg gaatttcgcc
     7261 gaggctggca aattggaaaa tgcggttgtt ttcgccgatg gtggtgtgac cgttgatgac
     7321 ggcgtgcgga ccgatttctg tattcgcacc gatttggacg ttggggccga tgacggtgta
     7381 cgcgccgact ttgacgccgg agtcgagttc ggctttgggg tcgatgacgg cggtcgggtg
     7441 gatgagggtc atgttttgaa tactccgctg cttgttaaga caaataatag aaaatcacag
     7501 tttgtcgatt tcatcgcaga ccgcaatcac ttgatcgcta aaatggctaa atactgaagc
     7561 agtatctaca tccatgtaat taagcagttc aaaaatagat tggtaaatat ttctagcgta
     7621 gccggtatct tttattgcac ctttaaaact aaaacaaatc ggttcgtgat gtgccaaacg
     7681 attacggaaa ttgttaatat tggaaagttc tcgaaaaatc catttttggt tatattgcac
     7741 acttggtgta gatttgggct tttttggaaa aactttcatc aatacttttc ctgcagcatc
     7801 aaattgagca tctttccctc ccgcaaacag ataccgccaa aaaccgaatc ctagtcctgc
     7861 aaccaattgg ttgtggtcaa actttcctcc gctattattt tgtattttag ataatgcaac
     7921 cttgattaat tcagcagatt ctttcgtacc atttcttaaa aagcaacctt gatatttcaa
     7981 tgcaggattc gtttgtggtt gtatgctatc atataaccgg tttctgtctt taaactcctg
     8041 ccgaaagcaa atatcaattt tgtttcttaa aacgatctca aatatagaaa caacatgaaa
     8101 aagttctctt gataaagata aattcaataa atacaattca gctgctttgt ctttcctaac
     8161 ggcattttta taccgttcca ttctgaaatc agaaaaaata ctacagcatt ccttataatt
     8221 cacgctaacc cttgattctt tatttttaaa ttgacgtttc aaactatatt tgatatagtt
     8281 agataaaact aatccccacc gtgtccctgg gtttgaataa acctaaaccg gtgggttttc
     8341 tttttattag gtcgtctgaa actttcagac gacctttttc cgaacgctca aaccacgcgt
     8401 ttggcgcaca taatcacggc ttcgacggcg acttgtccgt ccactttggc aacggcgttg
     8461 aatttgccga tgccgcgccg gctggtcagg agttcgactt caaagacgag ttggtcgccg
     8521 gggatgactt ggcgtttgaa acgggcttcg tctatgccgg cgaagaagaa aaattcgttt
     8581 tccttgcgcc cgccttcgct caaaatcgcc aacgtgccgc acgcctgcgc catcgcttcg
     8641 atgatgagta cgccgggcat aacgggcagg tcggggaaat ggccttggaa ttggggttcg
     8701 tttatggtta cgtttttgat ggcggtcagg gttttcatcg gctcgaaggc ggtgatgcgg
     8761 tcgagctgga gaaacgggta gcggtggggg atgagttttt ggatgtcttt ggcttcgatg
     8821 gggagttgta cgtccatgtc tgtcgtattc cttgaataaa gtcggtttgg atttattgcg
     8881 tgtctttgct gtctgaaagc tgctgttcca gcgttttgag gcgtttgttc atttcgctta
     8941 agcggtggat gtaaacggcg ttgcgcgccc attctttatg ggtggacatc gggaagatgc
     9001 cggcgatgtg tttgccgctt tcggtaatgc tgtgggtaac ggacgtgccg ccgccgatgg
     9061 tggttttgtc ggcgatttcg atgtgtccga ccgtaccgac gccgccgccg atgatgcagt
     9121 agctgcctat ggttacgctg cctgagatgc cggttttggc ggcgatgacg gtgtgcgaac
     9181 cgattttgca gttgtgtccg atttggactt ggttgtcgat tttggtgccg ttgccgacgg
     9241 tggtgtcgct catcgcgccg cggtcgatgt tggtgttcga gccgatttct acgtcgtcgc
     9301 ccagcgttac cgcgccggtt tgcgggattt tgaaccacga atcgccggcg aaggcgagtc
     9361 cgaaaccgtc cgcgccgatg accgcgccgc tgtggatttc gacgtgtctg ccgagtgtgc
     9421 agccgtaata aacgacggcg ttgggatgca ggacgacttc gtcgcccagt ttgcaatcgt
     9481 gttggacgac ggcgtttgcc aagatgcggc agccttcgcc gagcacggtg ttcgcgccga
     9541 tgtaggcgtt cgcgccgatt tcacagcttg cgggaacggt cgcgcccggt tcgacgacgg
     9601 cggtcggatg gatgccgccg cgcgctttga cgacgggtga aaacaggcgc gcgactttgg
     9661 cgaaatagag atagggatcg tcggcgacaa tcaggttgcg cccttcaaat ccgtctgctg
     9721 ctttggcgga aacgatgatc gcgcccgcgc tgctgtcgtg gacttcggct ttgtatttcg
     9781 gattggcgag gaagctgatg tgttccgcct gcgcgtctgc gagcgggcgc acggcggtaa
     9841 cggaaatgtc ctcgccgcgc cattcgccgc cgagccgcgc ggtgatttgg gacagggtgc
     9901 aggtggccgg aatcatggtt ttcctgtttg gtatgccgtc tgaaagggtt agcgggcgtt
     9961 catttcttta atgacgctgt cggtaacgtc gtattgggtg ttgacgtaaa tcacgtcctg
    10021 caaaatgaca tcgtaacctt cctgtttggc gattttgacg atgacgcggt tggcgttttg
    10081 ctggagggag gcaaactctt cgttgcggcg gaggttgtag tcttcttcaa actgcgcctg
    10141 ttttttgcgg aacgcttcga ccagcccgcg ccatttttct tcggcttgcg ccttttttgc
    10201 gtccttaagt ttgccgccgg cgagctgcct ttccaaatcc aagccttcgc gttgcagttt
    10261 ttgcaattcg tcctgacggg cggaaaattc gccgtccagc gttttttgga tgttgcgcgc
    10321 ctgcttggat tcgaggtaga tgcgctcggt gttgataaag ccgatttttt ggaaggtgtc
    10381 ggcgtgcgtg cctgtggtgc agcataaacc gatcagggtg gcggcaaacg cgcgggtcaa
    10441 acggatcatg gtaaaactcc ttcggatgtt gccgcgaaat gccgtctgaa gggcttcaga
    10501 cggcatttgc gggattagaa cgtcgtgccg agctggaatt ggaagcgttg gatttcgtct
    10561 tccggttttt tcttcagcgg gtaggcgtag ctgaatttca tcgggcccaa aggcgagagc
    10621 caggtaaccg cgccgccggc ggaatagcgc aattcgttgg taaaggtgga tttatgcgcg
    10681 ttttccgagt aaaccgattt gttgttaccg ttttcggcgg cggtataggt tctgccgtcc
    10741 cacacgctgc ctgcgtcggc aaacaggctc aggcggacgg tgcgtgcgtc tttcgccccg
    10801 ggcatcggga agagcagctc ggcggagacg ttggcttttt tgttgccgcc gtagctgatt
    10861 ttttcgccgt attcgtcata cactttcggg ccgagcgtgc cgctttcgta gccgcgcacc
    10921 gaacccaggc cgccgccgta gaagttttca aagaagggga tttctttggt tctgccgtag
    10981 ccgcccgcaa tgccgacttc gccgccgagc atcagcgtga aggttttgct taaggggaag
    11041 aaccaggttt ggttgtgggt ggcggagtag tattgcagtt tgctgccggg cagggcgatt
    11101 tcggcattta cgccggtcag gtagccgcgc gtcggccata aggcgctgtc ggtcttgttg
    11161 cgcccccagc cgacagtgcc tttgtacagc aggcctttga agctgccgtc tgcgccgtcg
    11221 gttttgccgt attgtttgat aaagtcggca tagcgtttgg gtgctttgtt gtaggtgttg
    11281 acggtcaggt gttccgccgc cagcccgaaa ttgacgcggt cgtattcggt aacggggata
    11341 cccatcctta cgccgccgcc ggcggtggtg gttttatatt gtttgacgct ggtcgatgct
    11401 ttgcgcgggt cgaaggcttt tccgtaaata tcgtagccca ggctgacccc gtctgccgtg
    11461 aagtacgggt cggtaaacga cagcgagccg ttgagcgtgg ttttgcttcg cgaggcgcgc
    11521 agggcggccg acttgcccgt accgaacagg ttgtcctgcg atacgccggc ggacatgacc
    11581 aagccggtat cctgaaccca gcccgcgctc aagtcgagcg agccggtgga gcgttcggtc
    11641 aggctcatgt tcaaatcgac tttgtcgggc gtaccggcaa gcgggacggc atcaaactgt
    11701 acgttgtcga agtagcccaa aagctcgacg cgctctttgg agcgttgcag cttggaggtg
    11761 tcgtaaggcg cggattccat ttggcgcaat tcgcggcgca cgacttcgtc gcgggttttg
    11821 ttgttgccgg tgatgtggat ttcgttgacg tagatttttc tgcccggttc gatgtgcagg
    11881 acgaaatcga cggttttggt tccggcgttc ggcagcggct gtacgctgat ttcgctgtat
    11941 gcgtagcctg ccgagcccat gcggttctga atctcaccca aaacggcggt catctgctgg
    12001 cgttcgtacc atttgccggg cttcatggtc agcagttttt ccagttcggc cttggggact
    12061 tcgttggtgt cgccttcaat cgacactttg ccccagcgga aacgtccgcc ttcgtggacg
    12121 gtgattttga tggtctgcct ggttttgtct tcgttggttt ggatgtcggt atcgaggata
    12181 cggaaatcga agtagccgtt gttctggtag aagtcggtta ctttttccat gtcttgggcg
    12241 aatttctggc ggtcgaaccg gtcgcttcgt gtcagccatg tccaaatgcc gccttcggtc
    12301 agcgacatct gccgcatcag tttgcggtcg gaatagactt ggttgccttc aaattcgatg
    12361 tcggtgattt tggcggattt gccctcgtca atcgtgatgt cgatgtcgac gcggttgcgg
    12421 gcgagtttgg ttactttggg cgtgatttgg atattgagtt tgccgcgccc gaggtattct
    12481 tctttcaggc cggcgactgc ctggttgagt gtcgcctgat taaagtattg cgactgcgcc
    12541 agcccgaacg attcgaggtt tttcttgatg gcgtcgtttt gcagcatttt ggcgccggtg
    12601 atgttgagcg agccgatggt ggggcgttcg ataacggtca gcagaagctg cccgtccgca
    12661 gtttcgactc gtacgtcgtc aaagaaaccg gtggcgtaca ggcttttgat gatggcactg
    12721 ccgtgtgtgt cgttgtaggt gtcgccgact ttgacgggca ggtagttgaa tacggtgctc
    12781 ggctcggtac gctgcaagcc ttcgacacgg atgtcttgga tggtgaagtc ggcaaatgcc
    12841 aaaggcgata tgcccaacat catcagtgcg gaggcaatct gtttcagttt catcgtaagt
    12901 tccttgtggt gcggaatgcg gtttcagact gcattccgaa acgtaaaatc taaccgatca
    12961 gccgggtaac gtcgttgaag aaggcggccg ccatcatcag catcatcagg gcgagcccga
    13021 agcgcaaacc gatgttttgg acacgttcgc ccaaaggttt gccgcgtatc cattcgacag
    13081 tataaaacac gaggtgcccg ccgtccaaaa cggggacggg cagcaggttc agcacgccga
    13141 ggctgatgct gaccaacgct aaaaattcca aataactttg caagccgagt tcggcggact
    13201 gtccggcaat gtcggcaatg gtcagcggcc cggaaatatg gctgacagag gcgttgccgc
    13261 tgattagttt gccgaaaaat ttgagggttg tccacgagtg ggaaacggtt ttttcccagc
    13321 ccatgccgaa tgcgcggaca acagacggac ggtagctgcg gcggatttgc gcgtcccacg
    13381 ccctgtccgg ctgcggacgg aggccgacgc gcccgatcag ggtgtggtcg ggctgttcga
    13441 cagtatcggg gcggatgtcg gcggtatggg tttgtccggc gcgttcgtag gtcagggtga
    13501 tttttttgcc cgggctttgg cgggtcaggt ttgcccattc ctgccatgag gcgatgggtt
    13561 tgccgtcggc ggcagtcagc ctgtcgcccg gtttcaggcc tgctttttcg gcggggctgc
    13621 ctttttccac gccgccggca acggttgtga ttttaaaggg catcagtccg atgtagcctt
    13681 ggttttttgc gattttaccg gcttccggcg tgcctgcggc atcgatggtg cggacggttt
    13741 gcgcgcccga tgccgtctga acgccgacgg cgactttgcc ggcttcgagg ttgaggacga
    13801 tttcggtttg cgcgctgctc cagtcttgga cggaaacgcc gttgacggat tgtattttgt
    13861 cgccgctttg gaagccggtg cgggcggcaa tggtgtcggg ttcgactgtg ccgacatagg
    13921 gccgcagttc ggttacgccg aaggaaaagc tcagtccgta cagcaaaacc gccagtgcga
    13981 ggttggtcag cggaccggcg gcgacgatgg cgatgcgctt ggcggggtgt tgtttgtcaa
    14041 aagcgtaggg taaatcggct tctgatactt cgccttcgcg cgtatcgacc attttgacgt
    14101 agccgcccaa cggaatcggg gcgaggcacc attcggtgtc gccgcgcttt cgggtgaaaa
    14161 acggtttgcc gaagccgacg gaaaaacgca caaccttgac gccgcacaac ctggcgacga
    14221 tgtagtgtcc gaattcgtgc aggctgacca aaatcaggat ggcgaagata aaagctagaa
    14281 gggtttgcaa atggttttcc tttgataacg gtgttcggac ggtatcagcg cagtgtgccg
    14341 ataaatgccc gcgcttgtgc gcgtgtccgg gcatcttgcg ccaacagtcc ttctatatcg
    14401 cccatgccgt ttgaaaagtc ttgtgcaaga cagtgggcga cggttttggc aatgtcggta
    14461 aacttaatct gtccgtccaa aaaggcggcg acggcggttt cgttggcggc gttcaatacg
    14521 cagggcgcgg ctccgcctgc gtttatggtt tcataggcga acttcaggca ggggaagcgg
    14581 ccgaagtcgg gcttttggaa ggtcagcgcg gacaatgcgc cgaaatcgag tttgccgaca
    14641 cccgaatcga tgcgctcggg caagcccaaa caataggcga tgggcgttcg catatcggga
    14701 ttgcccagtt gcgccagcac ggagccgtcg cggtagcgca ccatactgtg tatcacggat
    14761 tggggatgga tgacgacttc gagtttgtcg ggcggacagt tgaacagcca atgcgcttca
    14821 atcagttcca agcccttgtt tgccatagtg gctgaatcga cggagatttt gcgccccata
    14881 cgccaattgg ggtgtttgac cgcctgctcg ggcgtaatgc tgtcgaacgt gcttaaatcg
    14941 gttgttaaaa acgggccgcc ggaagcggtc aggataatcg aatcgatgcc gtgttcgttc
    15001 agacggtctg tgtaatcgcg cggcaaaact tggaaaatgg cgttgtgttc gctgtcgacg
    15061 ggcaacactg ccgcgccgtt tgcgcgggcg gtttccataa acaacgcgcc ggaaaccacc
    15121 agcgtttctt tgtttgccag ataaatggtt ttgccttttt gcgccgctgc gagcgcggaa
    15181 ggcagccccg ccgccccgac gatggcgcac atgacgccgc tgacttcgtc ggcagacgca
    15241 acgtcaacca atgcctgcgc gccgtgtaaa atctgagtcg ccgtgccgtc gcgtttcaac
    15301 agggcttcaa gccgggcggc gtgttcggca tcggcaacga cggcatattc ggggcggaac
    15361 gtttgacatt gagccgccag tttctcgacc tgcttatgcc ctgccagcgc gaatacgcgg
    15421 aatttttcgg gatggcggga gacaacgtcc agcgtgcttt cgcctatgct gccggtactg
    15481 cctaatatgg tcaggacttg tggtgtcata atgaggataa ctttataccg gatgccgtct
    15541 gaagcgtttt cagacggcat agaatcaatt taaaaccgac attatcgccg catagacgct
    15601 gataacggca atcaggctgt cggtacggtc gaacacgccg ccgtgtccgg gcagcaggtt
    15661 gctgctgtct ttgatgcctg ccgcgcgctt gagccagctt tccaacaggt cgccgcatac
    15721 gctgacaacg gtcaacacca aaccgattaa cacggtatcg aaccagcctg tatcgaatgc
    15781 cagccagccg gcacttcgta cggcagtcat gtacactgcc acgcaaaccg caccgccgat
    15841 tgcgccttcc cagctcttgc cggggctgat tgccggtgcg attttgtgtt tgccgagcgc
    15901 cttgccgctg aaatacgcgc aaacgtcggc aacccagacc aaacccatca cggcgagcag
    15961 cggcagggca tcatcgggat gcgggcgcag ggatacgagc gcgaaccaaa acggcatgag
    16021 caaaagccag ccgacggcat aaacctgcca accgccgttg agcctccatt tgaatctcaa
    16081 ccacaaaggc ataacggcga gccaaaatgc caaaacaaca taccaaacca aattaggcag
    16141 catccagccg cccgcatagg caactacgcc gaaaaccaag gttgcggcga ggtaatggtt
    16201 ggtttcggtt ttgcacaaac cggccatacg ggcatactcc cacaaggcgg tcagggcaat
    16261 cagcccgcaa aatgcagccc acagccattg cggcgcgtaa aacagcatgc ccagcatcag
    16321 cggcagcagc cacatagcgg ttattacccg ttgtttcagc atattcagtt cctttgctgt
    16381 ccgataggca gttgctcgga agtgcgtccg aaccgccgtt cgcgtttttg gaacgaagcg
    16441 acggcggcat ctaaggcggt ttcgtcgaaa tcgggccaca ggatgtcggt gaaatacagt
    16501 tctgcatacg ccatctgcca gagcaggaaa ttgctgatgc gcgtttctcc gcccgtgcgg
    16561 atgaacaaat ccggttccgg cgcgtcgccc agcatcaagt gtttggccag cgtgtcttcc
    16621 gtaatctcgg atacgccttc ggcaatcagt ttgtttgccg cctgcaaaat atcccagcgg
    16681 ccgccgtaat ccgcagcaat gctcagggtc agaccggtat tatttgccgt caaggcttcc
    16741 gcctcttcga taccttgcag aatctgccga ttaaagcgtt cgcggctgcc caatattttc
    16801 aggcgcatat tgttttcgtg caggcggcgt acctgttttt gcaaagcctg taaaaacagc
    16861 cccatcagga acgaaacttc gtcttcgggg cgacgccagt tttcggttga aaaggcaaac
    16921 acggtcagat attgcacgcc cagtttggcg caatgcttca ccatattttc caacgcgtcc
    16981 aagccgcgtt tgtgtcccat tatacgcggg agaaaacgtt ttttcgccca acggccgttg
    17041 ccgtccataa ttacggcgat gtgcctcggg atggcggtgt gttccaaaat ggtctgcgtg
    17101 ctgctcttca tatctgcctt tcgcggttcg gcgttcaaat gccgtctgaa cgccgcgccg
    17161 tgagatttaa accgccatca ggtcttcttc tttggcagcc aaaaccttat cggcttcggc
    17221 aatgtacttg tcggtcagtt tttgaaccgc ctcttcgccg cgacgtgcct cgtcttcgga
    17281 aatttctttg tctttgagga gttttttgat gtggtcgttg gcatcgcggc gcacgttgcg
    17341 gatagagacg cgtccttctt ccgcctcgcc gcgcacgact ttaatcaggt ctttgcggcg
    17401 ttcctcggtc agcatgggca tcggcacgcg gatcaaatcg ccgacagctg ccgggttcag
    17461 tcccaagttt gaatcgcgga tggctttctc gactttggcc gccatattgc cctcaaacgg
    17521 tttcacgccg atggtgcgcg cgtccaaaag ggttacgttg gcaacctggc tgacggggac
    17581 catgctgccc cagtattcga cttccacttg gtcgagcagg ccggtatgcg cgcggccggt
    17641 acgcactttc gccagatttt ctttcagtac ttcgaccgaa cgctgcatct tgccttcggc
    17701 tgttttttga atatcgttga tcatattgtt ctttcggtaa gataaggtag gcgggcagcc
    17761 gttcgaacgc gctccaagcc gttcagacgg cataaagacc gttaactgcg aatagtaccg
    17821 ttattcgggc atgacgacaa ggcagatgcc tgtaggaagg gtgtttcacc tttctagggt
    17881 ttttttaatt gccgctgcaa tttcgtaggc aaggttgacg gggacggcgt tgccaatcat
    17941 tttgtatgcg tcgttgacat tttgatagat gaatttgaag ttgtcgggaa agccttggat
    18001 tcttgcaact tcgcgtaccg tcatcctccg atatagtgtt tctttgccgg cagcaaaacg
    18061 gtagtcgttt gccccgtgct tttccatctt tggggcttgc gggtgcagtt ggcactgcct
    18121 gcctgaagct tggacggtaa aaccctgttc atcccacgct ttaactcggt tccggctcat
    18181 aaaaataggg gaaaaactgc cggtaaaata ttcgttgttg ttgactgcgt cggggttggt
    18241 cttgttttgc ggggcggaag gtacggctgt gtcctgcaaa tcccaaataa cgtctttcaa
    18301 tgtaatcttg tctttgtctt cgaccgtcga accttttgga aaagaaaatt ttatttccaa
    18361 gtctttacgg aaaccgatgt agaagaccct tttgcgttcc tgtgctacac cgtagtcttt
    18421 ggcgttggcc atagtcaagg ttacgtcgta tccgcatccg tcaaacattt tcagcaggtt
    18481 ttgtacggct ccgttgtggc ggtttgccag cattccgctg acgttttccg ctaaaaagaa
    18541 ttttggctgt ttgcttttca aaatacggat gtagtcgaaa aacaactgtc cgcgcgcatc
    18601 gtcgatgccg cgcaaagctc ccgcttcaga ccaagactgg cagggtgggc cgccgataat
    18661 cccgtcgatt tcttcaggga aatcttcttc tttaatcttg cgtatatcgc cttctatcaa
    18721 atgggtcttt ggatggtttg ccttgaaggt tgcccaaatg gttttatcgt attcgttggc
    18781 ggcggggatt tcaaatcccg ctttttcgaa acccaaatcc aaaccgccgc aaccgctaaa
    18841 caaactaatg attttcatag gattataagt ggtaggtgat gagttttgca tttcttaatc
    18901 gcgccgggtt gttgggattt ttaattttaa tgtcggaaat ggccagtttg gaatcctgta
    18961 tggcaagcag cttgtctgta ttgttaaaac tgttccattt gtcttcgttg ataatggcca
    19021 tgaaattgaa acttttttcc atattccgtt ggtaaatata gttgaagaca aaccatggat
    19081 tttcgattcc ccacataccc cgtactctca gataagttat gttgagcgga tcaatgcggt
    19141 tgaccctgcc cagctctttg gtttctgcaa actgtatgcc gccgatgttg ccgattcctt
    19201 ccttaatttg attttttatc ttcaaatagc attcggcatc ggcgcagtag tcgattccgt
    19261 aaaccattgc caagtgtttg aggtttttct ttttgtcaac gacgccgaca atatagataa
    19321 tgtctttctc ttcccatttt tccgcatctt tgcaggcttt tgtaagcatg ctgtcatcaa
    19381 cagagagctt tgattttggg tggctgctat tcagcgccag tgcggaatct ttactttcaa
    19441 ttttcttaac ttcgatggca tccccgtttc gcaacatggc atcaggaggg ttggagttgt
    19501 tgcccaaata tgagaatact tttgcgtgtc gggctatgcg ttgggtttca tttaggttga
    19561 aactgccgga aaacaagtct tttacatatt cttccaaagc atcacctgcc tgattggcac
    19621 gattgttgct ttgagaatgg gattcgaccc cgacaacagg gttgttagcc aaattgatga
    19681 tggcatcgat aatgttcatg gtttttcctt ggggttttgc ggcagattat agcaaaaccg
    19741 tcagggtgtt tatcggaccg atgccgtctg aagcggcagg cgtttcagac ggcatcgtgt
    19801 ccggccgtca gccgtgttcc cgtgtttcaa gcaggctttg gcgcaggtgt tggcgttcgt
    19861 gggcatccag ccatttgcgg cgggtgcgtt gcagcaggat gacgagggcg gaaatttcct
    19921 gacgcatatt ggtgctgagc cagaggaagc cctgccattg gtagtggagg tgttcggcga
    19981 gggcttccag ttcggggttg atggcggtgt cgatgcggat gcggcgggcg tgtctgccgt
    20041 tgatgagggc ggcggtttgt tgcaggtcgg tttggagcag tgtgaagtgg cggtcgagca
    20101 gccggatttc gctgccgttg agtttgggag attgcagctt ggcggcggtg gtcaggagca
    20161 gctcggtggt gttgacgatt ttgcggtggg cgtgctgcat ggcttccatc atggaggggc
    20221 tgatgcggct ttcgcccgat gtggcggcga ggtggctgcg gcttttgacc atgcgtgcgt
    20281 tgatttggcg cattttgacc atattctgct ccaaacgttc gcgcgtcata cgcctgccgt
    20341 tgctgatttc ggcaatcatt ttgctgcagt cggccaggtt gtcggcaagc atgaaacgcc
    20401 acatcagtgt ggatttcagc ggcagcagtt tggcggcggc aatggcgatg gcggcgccga
    20461 tgaggacgtt catcgcgcgc atcaggccgc tgtcgagcca ttcgctgccg ttgtcgccga
    20521 tgagcatgca catcgtcagc cccgccagca tagggacgta gccgtttttg ccgaccgccg
    20581 cccagccggc cagtgcgctt gccgtgccga tggtcaggta gaagaggagg ttgccgtgga
    20641 aataatgctg gttcagccat aaaacgccca aacccgcgcc cagcccgatg accgtaccga
    20701 gcatacgttc caccgccttg gagtaaatcg cgccttggaa ctggagcatg ccgaggacga
    20761 cgaagacggt catccctatc cattcgccgt gttggaggtg gagtagccgg gcgagtgcgg
    20821 tggcgaacag gacggctccg ccgagccgca cggcatgtat gaggcggcgg tggcggtagc
    20881 gttcgtagga gttgagccag cggccggaaa ggcgtttgcg ttgcgaggag ttcatatcgg
    20941 atgccgtctg aagcggaaat gtgaaaaagc acaggcttcc cgagggaggg tctgtgcttg
    21001 gtattggtgc cggagaaggg aatcgaaccc ccgaccttcg cgttacgaat gcgctgctct
    21061 accgactgag ctacaccggc gtttttcgtt atgatatata taaacggttg tttgtgcaac
    21121 ttttcgggcg ggcggcaagg cggtgcgcgg tataatgcgg cctgcttcgg aagagggggg
    21181 acggcgatgt ttgtgaacga gaaatatcct tatgcgaccc tgtttgcggg gctggtgttt
    21241 ttgacgctgc cgtttgcgtt ggcggtgcat gatgcctttg catgtgcgtt cggacgggcg
    21301 gggctgctgg tgtcggtgtc ggacggcgga ttcggccggc gtggcggttg ggacggcact
    21361 gtttggtttg tgttcggtgt gtttgcgttt ttgaatgtgg ttgtgtcggc gggcctgacg
    21421 aaactggcgt acaaaaagat gatgcggcgg cattcgcgtt acgcgctgtt tctgtcgggc
    21481 gtggcggctt gcgcggcggt ggcttggatt ttcaagctgt tgttgggcag tgccgccttg
    21541 ggcgggctga gcggggaggc ggtgtcggaa tatgcgtttg ccgtgtggct ggtgtctatg
    21601 ctgacgctgc cgaaacgcct gacgcgcgcg ccggtgcagc ctgtggtgtt ccacaggaaa
    21661 aaataggacg gaacggaaat gccgtctgaa acccgatacg cggtttcaga cggcatgttt
    21721 ttccgctaac attacgcctg aatatggaca ggaagcagat atggaacgta aagaacgcct
    21781 gcgtgcaggc attgccgcga tggggccgga tatttcggaa acggcgcagg acaggctttt
    21841 ggcctatgtg gatttgttga aaaagtggaa caaaacctac aatctgaccg ccctgcgcga
    21901 cgaggaaaaa atgattgtcc atcatctttt ggacagcctg acgctgctgc cctatatcga
    21961 aggcgcacag acgatgctgg atgtcggttc gggcggcggc cagcccggca ttccggcggc
    22021 ggtgtgccgt ccggatgtgc agataacctt gttggatgcg aatacgaaga aaacggcttt
    22081 tttgcggcag gcggctatcg agttggggct ggacaatgtg cgcgtggtgt cggggcgcgt
    22141 ggaggcggtt tcggacgtgc gtgccgacgt ggttaccagc cgtgcgtttg cggaactggc
    22201 ggattttgtg tcgtggacgg cgcacctgtt gaaagacggc ggctactggg cggcgatgaa
    22261 gggcgtatat ccgcaggggg aaatcggccg cctgccgcag gatgtgtgcg tcgaaaaagt
    22321 ccaaaggctc gacgtgccgg gcttggatgc ggaacgccat atcgtcatcc tgagcaagcg
    22381 ttgagcgcgc ttcagacggc atgaatacct tttttgtgcg gataaaggta aaattccgca
    22441 ctgtttttct ttttttcaac atcagacggg acacgggcgg gacatgagtg cgaacatcct
    22501 tgccatcgcc aatcagaagg gcggtgtggg caaaacgacg acgacggtaa atttggcggc
    22561 ttcgctggca tcgcgcggca aacgcgtgct ggtggtcgat ttggatccgc agggcaatgc
    22621 gacgacgggc agcggcatcg acaaggcggg tttgcagtcc ggcgtttatc aggtcttatt
    22681 gggcgatgcg gacgtgcagt cggcggcggt acgcagcaaa gagggcggat acgctgtgct
    22741 gggtgcgaac cgcgcgcttg ccggcgcgga aatcgagctg gtgcaggaaa tcgcccggga
    22801 agtgcgtttg aaaaacgcgc tcaaggcagt ggcggaagat tacgacttta tcctgatcga
    22861 ctgtccgcct tcgctgacgc tgttgacgct taacggcttg gtggcggcgg gcggcgtgat
    22921 tgtgccgatg ttgtgcgaat attacgcgct ggaagggatt tccgatttga ttgcgaccgt
    22981 gcgcaaaatc cgtcaggcgg tcaatcccga tttggacatc acgggcatcg tgcgcacgat
    23041 gtacgacagc cgcagcaggc tggttgccga agtcagcgaa cagttgcgca gccatttcgg
    23101 ggatttgctt tttgaaaccg ccatcccgcg caatatccgc cttgcagaag cgccgagcca
    23161 cggtatgccg gtgatggctt acgacgcgca ggcaaagggt gccaaggcgt atcttgcctt
    23221 ggcggacgag ctggcggcga gggtgtcggg gaaataggtc aatccaaatc gggctgcccg
    23281 tgcctttatg ctgtttggcc gggtgcgtta atatggcgga ttaaaataaa aatacttata
    23341 tcgtcattta tagcggatta acaaaaaccg gtacggcgtt gccccgcctt agctcaaaga
    23401 gaacggttcc ctaaggtgcc caagcaccag gcgaaccggt tccgtactat ctgtactgtc
    23461 tgcggcccgc cgccttgtcc tgatttttgt taatccgcta tatcgtcatt cccgcaaaaa
    23521 caaaaaaatc aaaaacacaa aactgaaata tcgtcattcc cgcgcaggcg ggaatctagg
    23581 tctgtcggta cggaaactta tcgggaaaaa cggtttttcc aaccctgaga ctccggattc
    23641 ctgttttcgc gggaatccgg ttttttgagt ttcagtcatt tttgataaat tcttgcagct
    23701 ttgagtttct agattcccgc ttttgcggga atggcggttt ggaagttacc tgaaattcaa
    23761 aaaaaaaaac ggaaaccgga cggattggat tcccgcctgc gcgggaatga cggattttag
    23821 gtttttttga ttttctgttt ttcgcgggaa tgacggttgg gaagtttccc gaaacttaaa
    23881 aacaactgaa acccaaaaaa ctaaattacc gcctgcgggg gaatgacggt tcgggttctt
    23941 tctctttgaa gttgcgatgc tggaaatgcc gtctgaagtt cagacggcat ttttgtgccg
    24001 gtttaaaaca aggcctgctg cgcgagcagg tttctgacgg gggcgaagtc gcggcggtgt
    24061 tcgggcagca cgccgtattg tttgagggct tccagatgct gctttgtgcc gtaacctttg
    24121 tgtttgtcga aaccgtattg gggacggcgt tgcgccagtg cgtacatttc cgcatcgcgt
    24181 gcggtctttg ccaaaacgga tgcggcggag atttcgatga ttttgctgtc gcctttgacg
    24241 actgcttcgg cagggatgcc caaatgttcg ggaatgcggt tgccgtcgat gaatattttt
    24301 tcgggacgcg cagccaaacc gtaaacggca cgtttcatgg cgagcatggt ggcgtgcagg
    24361 atgttgaggc ttgcgatttc ttcgggcgtg gaggcggcga cgtgccacgc gaccgcctgt
    24421 tctttaatca tttcggcaag cgcgtcgcgt tttttctcgc tgagtttttt ggagtcggtc
    24481 agtccgggca ggtcgaatgt ttccggaagg atgacggcgg cggcaaacac gctgccgacc
    24541 aaaggtccgc gtcctgcctc gtccacgccg gcggtcagta tgtgcatgat gtttcctgtc
    24601 gggatggtac gatgccgtct gaaaatggtt tcagacggca tcgtaccgat gtgtttattt
    24661 cgcgtcttta aacccgcgct tcaaatgcac catcagcagt gcgacggcgg caggcgtaac
    24721 gccggaaatg cggcttgcct gcccgacggt ttcgggtttg tgctggttga gcttttgttg
    24781 cacttcggcc gacaagcctt tgactctgcc gtaatcgatg ccgtcgggca gctttaaggt
    24841 ttcgatgtcg cggcggctgt cgatttcttc gttttggcgg tcgatatagc cttggtattt
    24901 gacttggatt tcgacttgtt cgaccacttc ggcggagagg ttttcagacg gcatcgcgcc
    24961 ttcgagcgtc atcagcgcgg cgtaatcgag gttcgggcgg cgtaacaaat cgtgcaggtt
    25021 ggcttcgcgg ctgagttttt gtccgaacac gcggatttgt tcgtcttcgg cgagtttttg
    25081 cggcgtgtac cacgttgttt tcaaacgttg gatttcgcgt tcgacggctt cgcgtttttc
    25141 gttgaacatg cgccattgcg cttcacccac caagccgatt ttgtagccgt cttcggtcag
    25201 gcgcatatcg gcgttgtctt ccctgagttg caggcggtat tcggcgcggc tggtaaacat
    25261 ccggtaaggt tcgttcaggc ctttggtgat gaggtcgtcc accaatacgc cgaggtaggc
    25321 ttgttcgcgg cgcagcagga gcggatcttg tccgcggacg tattgcacgg cgttcgcgcc
    25381 cgccagcaat ccttgcgcgg cggcttcttc atagcctgtc gtgccgttga tttgcccggc
    25441 gaaaaacagt ccctcaatgg ttttggtttc gaggcttgct ttgaggttgc gcggatcgaa
    25501 gtagtcgtat tcgatggcgt agccggggcg caggatatgg gcgttttcaa gacccttcat
    25561 actgcgcacg agcgcgattt ggatgtcgaa cggcaggctg gtggagatac cgttggggta
    25621 gtattcgtgc gtggtcagac cttcgggttc gaggaaaatc tggtggctgt ctttgtcggc
    25681 gaagcggttg attttgtctt cgatagacgg acaataacgc gggcccacgc cttcgatttt
    25741 gccggtaaac atcgggctgc ggtcgaagcc tgagcggatg atgtcgtggg tttgcgtgtt
    25801 ggtatgcgta atccagcagg acacttggcg cgggtgcatt tcggcgttgc cgcgcacgga
    25861 catgacggga acgggcgtgt cgccgggctg ttcggtaagt tgggaaaagt caatcgtgcg
    25921 tccgtcaata cgcggcggcg tgccggtttt cagacggcct tgcggcagct tcaattcgcg
    25981 caaacgtccg ccgagcgatt tggcggctgg gtcgccggcg cggccgcctt cgtagttttc
    26041 caaaccgatg tggattttgc cggacaagaa cgtgcctgcg gtcagcacca cggcgcgtgc
    26101 tttaaactcc gcgcccatcg cggtgatcac gccgctgatg cgttcgccct cgagtgttac
    26161 gtcttcgacg gcttgttgga aaaggtcgag gttttcttgg ttttccaaca tttcgcggat
    26221 ggaggcttta tacaggatgc ggtctgcctg cgcgcgcgtg gctcgtactg ccgcgccttt
    26281 gctggcgttc aggcggcgga actggatgcc ggatttgtcg gtcgccaacg ccatcgcgcc
    26341 gcccaatgcg tcaagttcgc gcacaagatg gcctttgccg atgccgccga cggaggggtt
    26401 gcacgacatt tgtccgagcg tttcgatatt gtgtgtgagc aaaagcgtct gcgcgcccat
    26461 acgggcggcg gcaagcgcgg cttccgtgcc ggcgtgtccg ccgccgacga cgatgacatc
    26521 gtaggttttg gggtaaatca tgtgggtcat gtgtgtattg cctatcggtt tttcagacgg
    26581 cattccaagc aggatgccgt ctgaaaaacg aaacggattc aaaagtaaag ggttgggatt
    26641 gtacgcctgt tcgccctgtt tttacagtgc gcggaaaggg aaaagccgct tcgcggggaa
    26701 gcggctccgg taagggcggg atttaccaaa catcggattt gatgcggcgt ttcaggcccg
    26761 gatgttcgga aagtttgaac tcggggtctt tgcccatttt cagcttggcg gtgtaatcgc
    26821 gcagcagcat aaacgccaat ggcgagagca gcaggatggc gacgaggttg atccacgcca
    26881 tgatgcccat cgccatatcc gccatatccc agaccaaagg cacattggca accgcgccga
    26941 aatagaccca cgccaaaacc agcatacgga aaacggcggt aatcagccaa tggcttttga
    27001 tgaattggac gttggactcg gcataggcat agttgccgat aacggtggaa aaggcaaaca
    27061 taaacaggat gacggcgagg aaacccgcgc cccattgccc cacttggctg acaatcgccg
    27121 cctgcgtcag cgccgcaccg ctcaaatcgc cataaggctg ttggtaaatc aagatgatga
    27181 aggcggtgca agaacaaacg atgatggtat cgacaaacac gcccagcatt tgaatcatac
    27241 cttgcgaaac agggtgtttc acttcggcgg cggcggcggc gttcggcgcg gaacccatac
    27301 ccgcctcgtt ggaatacagg ccgcgtttga tgcccatcat catcgtttgc gaaatcagac
    27361 cgccgagtaa gccgcctgct gccgcgtcga atttgaacgc gcccgaaaaa atctgaccga
    27421 acacgtccgg aatcatcgga atattggtca aaatgatgaa aagcgcgata aagaggtaca
    27481 aaaccgccat caggggcacg acgatttccg ccgctttgga tatgcgcctg atgccgccga
    27541 agataatcgg cgcggttaaa atcaccaggg cgacgccgac ataatgaggc tcccagcccc
    27601 acgccgcttt gacggtatcg gcgatggtat tggtctgaac cgcttcaaac acaaaaccga
    27661 aacagaaaat caggctcagg gcgaacaaca cgcccagcca tttctgcccc agaccgtgcg
    27721 tgatgtagta ggcagggccg ccccggaaat ggtggttgtc gcagtcgcgg actttaaaga
    27781 gctgcgccag cgaagattcg acaaacgccg aactcatgcc gattaaggcg gttacccaca
    27841 tccaaaacac cgcgcccggc ccgccgactt tgatggcgat tgccacgccc gcgatattgc
    27901 ccacgcccac gcggctggca aggccggtta caaatgcctg aaacggcgtg atgccgtgag
    27961 ggtcgtcccc ctgtttgcgg ccgccgagca tttctttgat gctgcgcccg aacaggcgga
    28021 attggacaaa gcccgtggtt acggtgaaga aaagccccgt acccaaaagc atataaacca
    28081 agtatgacca catcggatcg ttgatggcgc cgacccagcc gtgcagccat tcggtaaagt
    28141 tctcgttcat atcgcttcct taaagttgaa acccgcacat atggcggtat gcaagcaggg
    28201 tttaaatttt gtaaacgccc attctagcag attgtcaaca aaatcagaaa aatttacatc
    28261 gccgcgcgcc tgtggcgtta gaatcgcgtt ttgtttggag caaacacgat gaaacagcct
    28321 gtttttgccg ttacttccgg cgagcctgcc ggcatcggcc ccgatatttg tttggacttg
    28381 gcgtttgcac gcctgccctg ccgctgcgcg gtattgggcg acaaacacct gttgcgcgcg
    28441 cgcgccgaag ccttgggcaa aagcgtcgtc ctgcgcgact tcgatccgga atcaggcggc
    28501 gcgcacggag aaggcgggct ggaagtgctg cacatccctg ccgccgaagc ggttgaggca
    28561 gggtggctca atcccgccaa cgccgcctat gtgctgcaac ttttggacac cgcgctcgca
    28621 ggcatttcag acggcatttt cgacggcatc gttaccgcgc cgctgcacaa aggcatcatc
    28681 aacgccgcgc gcgcaagcac aggttttttc agcggacaca ccgaatatct ggcggaaaaa
    28741 agcggcacgg ggcaggtcgt gatgatgctt gccggcaaag gcctgcgcgt cgccctcgta
    28801 acgacccacc tgccgctgaa agacgttgcc gccgccatca cgcaaccgct gattgaaagc
    28861 gtcgcacgca ttttgcatca cgacttaaaa cacaaattcg gcatcaaaaa tcccaaaatc
    28921 ctagtcgccg ggctcaatcc ccacgccggc gaaggcggac acctcggaca cgaagaaacc
    28981 gacaccatta tccccgcgct cgaaaacctg cgccgcgaag ggataaacct cgccggcccg
    29041 tatccggcgg acacattgtt ccaaccgttt atgctcgaag gtgcggatgc cgtattggcg
    29101 atgtaccacg accaagggct gcccgtgttg aaataccaca gcttcgggca gggcgtgaac
    29161 atcacgctgg ggctgccctt tatccgcacc tccgtcgatc acggcaccgc gctcgatttg
    29221 gcggcaaccg gcagggcgga ctccggcagc ctgataactg ccgtggagac cgccgtcgag
    29281 atggcgcgcg gcagccttta aagatgataa aagacccgtc attcccgcgc aggcgggaat
    29341 ccggaccttc gggcggcggc aatattcaaa ggttatccga aagtttgagg ttctagattc
    29401 ccgttttcac gggaatgacg aaaggtggcg ggaatcacgg gaatggcgaa gtttcaggcg
    29461 gtattgcagg tatccgaaac catgcaaaaa gaggttctgc ggaacagaac ctctttttgc
    29521 cgccgtcggt tcagccttgc cgggtttcga cttggatcat ttcttcggca gggacggttg
    29581 cggcttcaga cggttttggc tgttcggaac ggcgcaaacc gcgtccggct tggacttcgg
    29641 gttgtgccgc ccatgccttc aatgcggcag ggtcggtttc gatcaggacg agtccgccgg
    29701 tttgtgcggt ttcccgtgcc tgttccgccg cagccgtaaa ggttgcggtt tcagacggca
    29761 tttcctgtgc tgcggctttc ggtgccgcgc cttcgggcag gatgtcggcg gcggcacggc
    29821 ggattttttc cgccgcatca taaatcagtg cgtcgccgtt tgaaacggcg gaagatgccg
    29881 tctgaacggg tgtggcgatg agcggatcgg caatgctgac ggtaatcggc gcgtttgcgt
    29941 cggtttcgcc gaaaacgtgc gcggcggcgg aacggacttt gtcggcggtg tcgtgaatat
    30001 tcaggtactg ctcgattttg gcggcagacg ggatattgcg ttttttgccg ttttgacggc
    30061 ggtcgcgctg attgttgcgt tcgcggcgtt ctttggcatc gcggctgtcg cgttcgcggc
    30121 ggttgcgttc ggatttgggc ttgctgcctt tgtcttctgc ggtatacggt tcgggcggcg
    30181 tgttttccgc tgtctgaacg gctgtttcgg caacggcggc ggtttccggt gcggtttggc
    30241 cacgttcgtt gcggctgccg ttgcggcggc gttttccggt ttgaccttcg gtttcgggcg
    30301 gtgcggcatc tgcaacgggt gcggcaggct gtacgttgcg gctttggatt tccgcttcgt
    30361 tggcgcgttc ggtggcacgg tcgccgcgtt cgttgcggcg gcggttgccg ttgttgcgcg
    30421 tttcggcttt gtcggcgcgc gctttctgtc cggcagtttt gcccgccgct tcgcggactt
    30481 ctattttgct gccttcgcgt ttgctgcggc gcgggttttg gcggcggttg ttggcgcggc
    30541 tgccgctgcg gtttgccgtg ctgcgttttt cggaggtttc ggcagcgggt acggcttggg
    30601 tttcgctgcc gccgaaaatg cgtttgagcc atgctttgaa gctgtcccac caggaggttt
    30661 ttttctcggg ggcggcagtc ggggcggggc tggtgtggcg cacgcctttg acggcgggtt
    30721 cggggcgggc ggctttggct ttttcgccgc cgaaaggttt ggcggattcg tcttcttcag
    30781 gctcggcaac gcgcttgtag ctcggttcgc cgtcttcttc tacgtcgtcg gtgcggatgc
    30841 ggttgatttc gtagtgcgga ttttcgaggt ggatgttcgg aatcaggacg acgttcacat
    30901 ccaaacgctc ttccattgca aacagctcgg cgcgtttttc gttcagcagg aaggtggcga
    30961 catcgacggg cacttgggcg cgcacttcgc cggtgttgtc cttcatcgct tcttcctgaa
    31021 ccatgcgcaa aacgtgcagg gcggtggatt cgatgccgcg gatcacgccg gtgccggcgc
    31081 agcgcggaca ggcggcgtgg ctgctttcgc ccaaagcggg tttcaggcgt tggcggctca
    31141 attccaaaag tccgaaacgg gagagtttgc ccatctgtac gcgggcgcgg tcttttttga
    31201 gtgcgtcgcg caggacgttt tccacatcgc gctggtgttt ggggttttcc atgtcgatga
    31261 agtcgatgac gaccaagccg cccaagtcgc gcaggcgcat ttgtcgggcg acttcttcgg
    31321 cagcttccat attggttttg aaggcggtgt cttcgatgtc cgcgccgcgt gtggcgcgtg
    31381 cggagttcac gtcgatggag acgagggctt cggtgtggtc gatgacgatc gcgccgccgg
    31441 agggcaggct gacgctgcgc gagaacgcgc tttcgatctg gtgttcgatt tggaagcggg
    31501 aaaacagcgg cgtgtggtct tcgtagagtt tcagacgacc tgcgttgccc ggcatgacgt
    31561 agctcatgaa ctcggcaact tggtcgtaaa cttcttgatt gtccaccaaa atttcgccga
    31621 tgtcgggacg gaagtagtcg cggatggcgc ggatgagcaa cgagctttcc ataaagagca
    31681 ggtaggggtc gtgatgcgct tttcctgctt cttcgattgc ctgccagagt tgtttgaggt
    31741 agttcaaatc ccattccaac tcttccgcgc tgcggccgat gccggcggta cgcgcgatga
    31801 tgctcatgcc gttcggaatg tcgagttgcg ccatcgcggc tttgagttct tgacgctctt
    31861 cgccttcgat acggcgggat acgccgccgc cgcgcggatt gttcggcatc aataccagat
    31921 agcgcccggc gaggctgatg aaggtggtca gcgccgcgcc tttgttgccg cgctcgtctt
    31981 tttcgacttg gacgatgact tccatgcctt ctttgagcac gtcctgaatg cgcgcgcgcc
    32041 cgccttcgta gccgaggaaa tacgaacgcg atacttcttt aaacggcaaa aagccgtggc
    32101 ggtcggttcc gtaatcgacg aaacacgctt ccagcgacgg ctcgatgcgg gtaatgatgc
    32161 ctttgtagat attgcctttg cgctgttctt tgcccagcgt ttcgatgtcc aaatccagca
    32221 ggttttgtcc gtcgacgatg gcaacgcgca gctcttcggc ctgcgttgcg ttgaataaca
    32281 ttcttttcat gatcacctcg tggacaggcg gcattcgggc gcgccggtcc ggttcggcat
    32341 tccgtaaggc tgggtttccg atgtcttcgg ataaaaccgg taatcagttt ttgagttgaa
    32401 aatccgcagg gatgcacgtt ccggggaacc gtgtgcggaa gggtcggata aagaaggcta
    32461 taaagatcga tgcggcggtc tgcctgccgc gttccgaacg ctgcggtcgg aaaaatgggc
    32521 gccggcttct tcttgttatc gtgatgcctg tgttttcggg cggtttgcgt tcggggcttg
    32581 gggctccgct gccgtcttac ttccgcgccg aaacggcaaa atcaattcaa acttgactac
    32641 gttctgcgcc tgccggctgg gaacaggcgc agggaaaatg ctttgcggag tgcgttttta
    32701 atataaaatt ccgttttaaa gtaaaccgtt tcagggggcg cggcggacgc gctttctgct
    32761 gaaacggatg ttcggattat agatgaaaac gcacgaaata agcaaaggtt cggtcagcct
    32821 gataggggtt gccgaacatg aggcgggtca acgccttgat aactatctga taaaaatcct
    32881 caagggcgtg cccaagggct atatccaccg cattatccgc gccggcgagg tgcggttgaa
    32941 caagaaacgc tgcaaacccg acagccgtat tgcggaggga gatacggtgc ggattccgcc
    33001 tgtgcgcgtg gcggagaagg aaatgccgtc tgaaaggcgt gccgccgtac cggcgcgtgc
    33061 gtttgaggtg gtgtatgagg acgacgcgct actggtggtc aataagcctt ccggtgtggc
    33121 ggtacacggt ggcagcggcg tgagtttcgg cgttatcgaa cagttgcgcc gcgcccgtcc
    33181 ggaggcgaag tatttggagt tggtccaccg tttggacaag gatacgagcg gcttgttgat
    33241 ggtggcgaag aaacgcagcg cgctcgtcaa actgcacgaa gctatccgta acgaccaccc
    33301 caaaaaaatc taccttgcgc tgggggtggg caaactgccg gacgacaatt tccatgtcaa
    33361 actgcccctg ttcaaatata ccggcgcaca aggcgaaaag atggtacgcg tcagcgagga
    33421 cgggcagtcg gcgcatacgg tgttccgtgt gttaagccgt ttctcagacg gcattttgca
    33481 cggtgtcggg ctgtcgcacc tgactttggt gcgggcaacg ctgaaaacgg gccgtacgca
    33541 ccaaatccgc gtgcatctgc aatctcaagg ctgtccgatt gcgggcgacg aacgctacgg
    33601 cgattatcag gcgaaccggc gtttgcagaa attgggtttg aagcgcatgt ttttgcacgc
    33661 gtccgagctg cacctgaacc atccgctcac gggtgagccg ctggtgttga aggcggagcc
    33721 gccgccggat ttggcgcagt ttgcggtgat gttggaaaac gggacgaaaa tgtgaacccc
    33781 gatgccgtct gaaggcttca gacggcatcg gggcgtgaaa
//
LOCUS       DAXDZG010000017        30282 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXDZG010000017 DAXDZG010000000
VERSION     DAXDZG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30282
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.017"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB78_000690"
     CDS             complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB78_000690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC1268737.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB78_000691"
     CDS             complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB78_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC1268738.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB78_000692"
     CDS             complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB78_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC1268739.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3153..4646
                     /gene="rng"
                     /locus_tag="ACFB78_000693"
     CDS             3153..4646
                     /gene="rng"
                     /locus_tag="ACFB78_000693"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC1268740.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4681..5931)
                     /locus_tag="ACFB78_000694"
     CDS             complement(4681..5931)
                     /locus_tag="ACFB78_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1268741.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVGPDSKSET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLRADGDDDYYRAYIRTTEQGRIAV
                     MQENEYREDLAEDAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LPALSVYNIPSEIRGFVTAINLLLKRVDEDIRRRQRFVADAAHELRTPMTALSLQAER
                     LNNMPLPPDAGRQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLVLEKRQDIGVAVGGDVEVSADETEIYTLVKTFADNAVRYTPNGGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESECARVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6013..6642)
                     /locus_tag="ACFB78_000695"
     CDS             complement(6013..6642)
                     /locus_tag="ACFB78_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC1268742.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(6910..8223)
                     /locus_tag="ACFB78_000696"
     CDS             complement(6910..8223)
                     /locus_tag="ACFB78_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC1268743.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8592..9164)
                     /locus_tag="ACFB78_000697"
     CDS             complement(8592..9164)
                     /locus_tag="ACFB78_000697"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC1268744.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPEVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9323..10732
                     /gene="dacB"
                     /locus_tag="ACFB78_000698"
     CDS             9323..10732
                     /gene="dacB"
                     /locus_tag="ACFB78_000698"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC1268745.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10797..11966)
                     /gene="metK"
                     /locus_tag="ACFB78_000699"
     CDS             complement(10797..11966)
                     /gene="metK"
                     /locus_tag="ACFB78_000699"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC1268746.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12149..12259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12312..13205
                     /locus_tag="ACFB78_000700"
     CDS             12312..13205
                     /locus_tag="ACFB78_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC1268747.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13250..14314)
                     /gene="tsaD"
                     /locus_tag="ACFB78_000701"
     CDS             complement(13250..14314)
                     /gene="tsaD"
                     /locus_tag="ACFB78_000701"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC1268748.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14430..15617)
                     /gene="ccsB"
                     /locus_tag="ACFB78_000702"
     CDS             complement(14430..15617)
                     /gene="ccsB"
                     /locus_tag="ACFB78_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC1268749.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15610..17562)
                     /locus_tag="ACFB78_000703"
     CDS             complement(15610..17562)
                     /locus_tag="ACFB78_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC1268750.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17840..18463)
                     /locus_tag="ACFB78_000704"
     CDS             complement(17840..18463)
                     /locus_tag="ACFB78_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC1268751.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18670..19299
                     /gene="yihA"
                     /locus_tag="ACFB78_000705"
     CDS             18670..19299
                     /gene="yihA"
                     /locus_tag="ACFB78_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC1268752.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19419..21815)
                     /locus_tag="ACFB78_000706"
     CDS             complement(19419..21815)
                     /locus_tag="ACFB78_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC1268753.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLPQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            21967..23082
                     /gene="pilM"
                     /locus_tag="ACFB78_000707"
     CDS             21967..23082
                     /gene="pilM"
                     /locus_tag="ACFB78_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC1268754.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSAEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23085..23684
                     /locus_tag="ACFB78_000708"
     CDS             23085..23684
                     /locus_tag="ACFB78_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC1268755.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23685..24332
                     /locus_tag="ACFB78_000709"
     CDS             23685..24332
                     /locus_tag="ACFB78_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC1268756.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24350..24895
                     /locus_tag="ACFB78_000710"
     CDS             24350..24895
                     /locus_tag="ACFB78_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC1268757.1"
                     /translation="MKHYALLISFLALSACSQNSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            24914..27109
                     /gene="pilQ"
                     /locus_tag="ACFB78_000711"
     CDS             24914..27109
                     /gene="pilQ"
                     /locus_tag="ACFB78_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC1268758.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
     gene            28377..28889
                     /locus_tag="ACFB78_000712"
     CDS             28377..28889
                     /locus_tag="ACFB78_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC1268759.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            28969..30048
                     /gene="aroB"
                     /locus_tag="ACFB78_000713"
     CDS             28969..30048
                     /gene="aroB"
                     /locus_tag="ACFB78_000713"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC1268760.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT         7654 a         8789 c         7316 g         6523 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaaaatca gatttccgtt acactttttt
       61 ctaatatttc agacggcatt ttgctcacac acccaaataa ccttccctgc cggaaagcca
      121 ccttgccaaa tgcgcttcga cgatttcagg gttttgttca atcagcatcg gggcggtttc
      181 gcgcgcttgt tccaaaaggt gtaagtcttc ttcgagcttg gcgaagcgca gcataggcac
      241 gccgctttgg cgcgcgccga gaaattcgcc ggggccgcgg atgttgaggt cttggcgggc
      301 gatttcgaag ccgtcggtgt gttcgtagat gactttcagc cgcgctttgg cgagttcgcc
      361 caagggttcg gcaaacagga ggacgcacac gctttccgcc gcgccgcgcc ctacccgtcc
      421 gcgtaattgg tgaagctgcg ccaagcccat gcgctcggcg tgttcgatca ccatcagggc
      481 ggcgttgggc acatctacgc cgacttcgat gacggtggtg gcaaccaaga cgttcagacg
      541 gcctgcggca aattccgcca tgacttcagc cttttcggcg gccttcatgc gcccgtgtac
      601 cagtccgata ttgggttcgg gcaatgccgt ctgaagccgg gcgagggttt cggcggcggt
      661 ttgcagttgc agggtttcgc tttcttcaat caatgggcag acccaatatg cctgccgccc
      721 ttttcggcaa gtgccgagga cgaagccttc gacttcggcg cggcggacgt tgttgacgag
      781 gcgcgtttta atcggcgtgc gcccgggcgg caattcgtcg atgacggata cgtccaagtc
      841 agcgaagaaa ctcatggcga gcgtgcgcgg gatgggtgtg gcggacatca tcagctgatg
      901 gacttcgcgc cctttgtttt tgagtgcgag gcgttgtgca acgccgaaac ggtgctgttc
      961 gtccacaatg ctcaagccca aattgtgaaa cgccacgccg tctgaaaaca gggcgtgcgt
     1021 gccgacggcg attttgacgc tgccgtcggc gagtttggct ttggcttcgt ctttggcttt
     1081 tttgcgcaaa ctgccaaaaa ggcagacaac ttcaatgccc aaaggttcga gccattgttt
     1141 gaacttgatg aagtgctgtt cggcaaggat ttcagtgggc gccattacag ccacttgcgc
     1201 gcctgattcg atggcggtca gcgcggataa ggcggcgacg atggttttgc cgctgccgac
     1261 atcgccttgc agcaggcggt gcatggggtg ggtttgcgcc atatcgcggc agatttcgga
     1321 aacaactttt tcttgtgcat cggtcagggc aaacggcagg gcttggcgca gggcttgggt
     1381 caatgtgccg tcgccgcgca atgccgccgc cgtgccgctg acgcgcttct gccgcgccaa
     1441 acgcatggac aactgttgcg ccaaaagttc gtcgaatttg agccgctgcc acgccggcag
     1501 cgtgccgtct gaaagttgat gaatagtgaa actcggcggc ggcgaatgca aaaggcgcag
     1561 gctttcggca aggcgcggca gcttcagacg gcctaataaa gcatcaggca aggtgtcgtg
     1621 cagcggcgta acgtccaacg ccgtctgaat gatgcggcgc aaagtgggct ggttcaaacc
     1681 gtttacggtc gggtaaaccg gcgtgaggct ttccgccaaa ccgccgccct cggcatcgcg
     1741 gattttggga tgaatcatct cgtcgccgta aaatccgtgt ttgatttcgc ccacggcgcg
     1801 gatgcgtttg ccgaccgccg tctgcttctg atggctggcg taaaagtgga tgaagcgcag
     1861 aaaaaggacg ctgccggaac cgtcggcaat ttgaacaatc agctgcttgc gcggtttgaa
     1921 cgttacttcc tgatggataa cctcgccctc gacctgacac ggcacgccaa tcggcgcgtc
     1981 cttaatcggc ataatgtgcg tctcgtcctc gtaacgcagc ggcaggtgca acaccaaatc
     2041 ccacgcggta tggaggttga gtttgtcgag cttcttggcg gaaacatcgg tgattttgag
     2101 ctgtttctgg gtttcgggcg acatcatagg cagattcctt tggacgcgcc tattttatcc
     2161 gaaaacaaaa atgccgtctg aaacggattc agacggcatc ggcaggcagg aatcaagccc
     2221 cggcggcttc ggcttcctgc tgttgttggt aaatcgcttc aaaattaatc ggcgcaagca
     2281 ggacgggcgg gaagccggcg cgcgttaccg taccggaaac cgcttcgcgc gcgtaaggga
     2341 agaggatgtt cggacacgcc acgcccaaca gcagctgcac atcttcttcg gggatgtttt
     2401 ccagacggaa aataccgctt tgggttactt cgttcaaaaa catcgtgcgc tcgttatcca
     2461 atttggcggt aacggttacg gtaacgtcca cgtcgtagta gccgtcttcc agcttttgac
     2521 tgccggtgga aacgcgcata tccacttcgg gatcgccctg ttccaaaaag atttgcggcg
     2581 cgtgcggcac ttccaaagac aagtctttga catacagtcg ctcgatgctg aatacgggtt
     2641 gcagttcttc gctcattttg ttttcctagt tgtgggttaa gggttcagca gtccgtccag
     2701 cccgccttcc tgctgaaggc ggtagaggtc ggtaaatccg ccgacgtgcg tttcgccgat
     2761 gaaaatctgc ggcacgctgc gctgtcccga aagccgctgc atttcggcaa aggcttcggg
     2821 gcttgcatcg acacggattt cgtcgatatg tccgacacct gccgcgtgca gcagcctttt
     2881 cgccatcgcg cagtaggggc aaaacggacc tgtgtacatg gtaacggtct gcatattggt
     2941 tttccgaaag ttttgcaatg ataatcaata taggggcaat cgctccgttt ggcaagtgcg
     3001 gaacagatgc acgttcagac ggcatgtgcg gagtgtgtca aagtttcttt tttaaagtat
     3061 gataggcatt gtgaaaaata tttttgcacc cgctctgcgc ggcggggcgg aacggatgca
     3121 aaaatatttt tattacattt tcaggaaaaa ccatgttgtc aggactcccc atccccaaag
     3181 acatcgcgcg cccgcccgaa acgatattgg tcaacatcac gccgcaggaa acgcgcgtgg
     3241 cggtgttgga ggaaaacaat atctgcgagc tgcacatcga gcgcaacagc gggcacagcc
     3301 tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct gcccgggatg cagagcgcgt
     3361 ttatcgacat cggcttggaa cgcgcggcat ttttacacat cgtcgatgtc ctcgaacaac
     3421 gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct gtttgaaggg cagtcggttt
     3481 tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc gcggctttcc acccaaatct
     3541 cgctggcggg gcgtttcctc gtccaccttc cgcaagaaga ccacatcggc gtgtcccaac
     3601 gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg cctcgacaaa ctcctgccgg
     3661 aaaatgcctg ccggggctac atcatccgca ccaacgccga aaacgccacc gacgaacagc
     3721 tccagtccga catcgactac ctgaccaaag tgtgggaaca catccaagaa caggcgaaaa
     3781 tccggccgcc ggaaaccctg ctttatcagg atttgccttt aagcctgcgc gtgttgcgcg
     3841 atatggtcgg ctgcgacacg cagaaaatcc tcgtcgattc caccgtaaac cacgggcgca
     3901 tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg caggatagaa ttgttcaaag
     3961 gcgaacgccc gctgtttgaa acccacaaca tcgaacagga aatcagccgc gccctgcaac
     4021 cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga gtccaccgaa gccatgacca
     4081 ccatcgatgt caacaccggc ggcttcgtcg gcgcgcgcaa tttcgacgaa accatcttcc
     4141 gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt aaggctgcgc aaccttggcg
     4201 gcatcatcat catcgacttc atcgatatgg cgcaggaaag ccaccgcgaa gccgtgttgc
     4261 aggagcttgc caaagccctc gccttcgacc gtacccgcgt taccctgcac ggttttacca
     4321 gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga aaacttaaac caagtcctct
     4381 gcgaaccctg cccttcctgc caaggcaggg gccgcctgaa aacgccgcaa accgtatgct
     4441 acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta cgatgccgaa agtttccgca
     4501 tcctcgccgc ccccaacgtc atcgatttgt ttttggacga agaatcgcaa tccttggcaa
     4561 tgctgataga tttcatcggc aaaccgattt ctttggcggt cgaaaccgct tacacgcagg
     4621 aacaatacga tattgtgctg atgtaaaaaa tgccgtctga agccttcaga cggcatctgt
     4681 ctatttcagg gtttccttgt ccaacagcgc gcgtatcaac agcccgtgtc cgaaacgtcg
     4741 gctgtcggtc agttccaaat atccgccgta ttttttggcc agcgtgtcgg caatcgacag
     4801 ccccagcccc gccccctgct gctccgttcc caaaatacgg taaaacggat cgaggacgcg
     4861 ggcgcattcg gattcgggaa tgccgttccc gttatcctcc acccacacgg cgagatattt
     4921 cccttcgtcc gtgaaaccca aatctatcct gcccccgttc ggcgtgtaac gtaccgcgtt
     4981 gtcggcaaag gttttaacca gcgtatagat ttccgtttcg tcggcagaca cttcgacatc
     5041 gcctccgacc gccacaccga tgtcctgacg tttttccaaa accagcggca tcagttcctg
     5101 caacacttgg cggaaacggc tttgcagacc gaatgtcgtt ttcgtcaaag gggtttcgtc
     5161 cgactgcgaa cgcgccagtg ccaaaagctg ttcgagcagg tgtttgttgc gcctgatgct
     5221 ctgctgcaaa acggcggact gccgccccgc atcgggtggg agcggcatat tgttgagccg
     5281 ttccgcctga agggaaaggg cagtcatcgg cgtacgcaat tcgtgtgccg cgtcggcgac
     5341 aaaccgctga cggcggcgta tatcttcatc aacacgtttc aaaagcagat tgatggcggt
     5401 tacgaaccct ctgatttcac tgggaatatt gtacacactc aaagcaggca ggccgttgat
     5461 tcggcgttgt tcgagacttt gcgacaattt gcggacgggg cgcatggctt tgtgcgtaat
     5521 ccacacagtc agcagtatca tcagcggcag tgccgccaac aggggcaaca cgctttgccg
     5581 tgccgcatcc tccgccaaat cttcacggta ttcgttttcc tgcatgacgg caatccgtcc
     5641 ctgttcggtc gtgcggatat aggcgcggta ataatcgtcg tccccgtccg cccgaagcgt
     5701 gtgcagaccg tccgccagat gcgcgggcag gctgacaaca gggtcttcct gctgcggcat
     5761 ctgtaccaaa atacgcgtat cgccgtcgcc ttcgggcaaa gtttcggatt tggaatcagg
     5821 gccgacatac aatgccgcct gacggagcag gtcgtcctgc aacgcttccg tttcgtggaa
     5881 ggtttcgtag taggaaaaca tacctgcaag cattgccagc ggaacaaaca tccaaatcag
     5941 ggcaaggctg attctgacct gaagcgaatg ttttaaaatt ttaaaaaaac ggtccggcat
     6001 cttcgttcct gttcagacgg catcttgacg cggcatcagc cagccgacac cgcgcacatt
     6061 ttgtatgctt tccttaccca atttcttgca cagcccgtga atcagaaaat ccaccgcatt
     6121 gctttcgact tccccgcccc aaccgtaaac cttgtcctcc gaatccgagc gcgacaaaat
     6181 cacacccggc ctcgccagca aagcctgcaa taccgaaaac tcctggttgc tcaatgccac
     6241 ctgcctcccc tcggcaataa tttctacctg atacgttgca ggattgaggg ataacgcacc
     6301 atttgacaga cacgcctgcg cctgtccgct cccgcgccgc aacaccgccc gcatccgcgc
     6361 cttaaactcc gccatatcga acggcttgac gatatagtca tccgcaccgc cgtccaaacc
     6421 gttcagccgg ctgtacaaat catcccgcgc cgtaacgatc agcacgggga cggtacagcc
     6481 tgccgcgcgt atttccgaca aaacatccaa accgtcccgc ccaggcaaac ccaaatccag
     6541 cagcatcaag tcataaggct gggcagcggc agccgccgca acctgcgcgc cgtttttgac
     6601 ccaatccacc gcatagccgc cgtctttcaa acttgccgac accgcttacg caatcatcgc
     6661 attgtcttcc accagcaaaa cacgcatcaa ctttcccttc aaaataaacc gtgcctattc
     6721 taacacccca aaattagccg caatttagcg gcctttacgc ttgccggtat ttttcaaaac
     6781 tgcggcacaa aaaaaccgcg ccggcaactg ccttcagacg gcattggggc gcgattgcaa
     6841 cacacggaca ggcaggcaaa gcctgcgaca gatcacagga acgattcggg cttcaggcgg
     6901 cttcgccgtt tacggcagag gcacgattcc tgccgctatc gaactgatca atatcgccag
     6961 cgacaaaccc cacgcccaga aaaacgaata acggatgtgt ttgcccatcg acaatttcgc
     7021 caaacccaaa cccatccaca aagccggcga aagcggcgta acaaaagtac cgacgatact
     7081 gccgatcaac atcgcatagc ctgccgcttc gggtgcaacg cccgcctgcg aggtaatctg
     7141 ttccacaatc ggaaacagtc cgaaataata agcgtccgta ctcaaaacca actcaagcgg
     7201 aatacccaac acaccgatgg caatatgcag ataaggcagc aacgcgtccg gcaggatatg
     7261 cacaaggtct ttggaaatcg cgtccaacat ccccgcgccc ttcaaaatcc ccaaaaacgt
     7321 acctgccgcc aaaataatgg acgccatcat caccgcgccg ccggcgtggg cataaatccg
     7381 ctccatctgt tcctgcgggc tgcggtaatt caaaagcaac gccgccgttg cagccagcat
     7441 aaatacataa cccggcggga agatgcccga aaaaagcagg ctcatcgccg ccaaaaacag
     7501 caggacattc caccaaaaca gtttcggacg cgccaatttt tgttcttctt ccgacaaagg
     7561 cgccggcttt atcaaatccg ccacggcggg caacgcgccc aactcccgga caatccgcct
     7621 tttttcacgc acacccaaaa acagggacag cacaaggata aacaccacac cgataatttg
     7681 caccgtcaac aaaggtttat acaattcgcc cacatctgcg cccaacacgc ttgcaacccg
     7741 cccgatcggc ccgccccgcg gcaaaaggtt gattagcccc gcgctggaag tcagcagcaa
     7801 aaacagcagg taaggattca tatgcagacg cttgtaaagc ggcaaaaggg cggggacgac
     7861 cgataaaaac gtcgtcgcgc ccgccccgtc caactgtgcc accaccgaca ccaagaccgt
     7921 ccccacactc actgccacga tattaccccg agtcagctta atcaaaccgc ctatcatcgg
     7981 acggaacagc cccacatcgt tcatgattcc aaaaaacaaa atggaaaaca taaacataat
     8041 cacaatctgc gtcaccgatt tcgtgccgcc cgaataaaat tcttttaatt gggatacatc
     8101 aaaccccgcc agcaacgccc caatcagcgg caccaagatt aatgcgatga tgggcgacac
     8161 tttttccgtc agcaacagcc atacgatgac cccgataatc agcaatccga taaacgtcag
     8221 cattatttct ctttatttta ttttgaacat aaaaccgccc gtgcaggcaa aaccgccaac
     8281 atatgcggga taagccttgc attccattca cgcctgccga catttctgca tccgtccgtc
     8341 atttcacggc ggcaaccgat acggaacaac cggtatcggg acggcgcggg ggcatccgtc
     8401 ccggtgcgcc gattcaaacg aaactgcccc ctatcattgc ggagcgcggg gcgtgccgta
     8461 cacgcgggat tttaaacacg ccgatcattt gccgcgcatt atccggcaaa cggcaaacct
     8521 tgacgctgcc cggcccatat aaaaaagccg caaaacccga accggttttg cggcttttcg
     8581 atttggtttg tttatttggc atttctttca atcaaaccga caaactgatt gatatatgac
     8641 tgaacaaaat cccttgccga gtcaatcaac ttaccgtttt catcaaacag cgtcggcgaa
     8701 ttgcccaaaa atacttccgg ctgtccggtt acgggcatat caaaatacga cagggcaagg
     8761 cgcaggtttt tttgggaact gtaaccgccc atcttgccga cggaatggct gatgatgccg
     8821 gccggtttgt ttttccaagc cacgtcggca ttcggtttcg agccgatgtc caccgcattt
     8881 ttcaaacagg cgggaatggt gcggttgttt tcggacgtaa cgaacaaaat gccgtccgaa
     8941 gccttaatcg tttcgcggaa agccgtgtag ctttcgggca gcggcacatc ttccacctca
     9001 gggtcgtcat aatcgaaatt gtaaagcggc agatgtccga tttcgacgat ttccgcctgc
     9061 cagccttcgg ggaacatctc cgccgcattc aatgccactt tgcgcgcaaa cgaagcacgg
     9121 cgcaggctgc ccaccaaaat actgattttc ttagccacaa tcattcctcc tgaatattaa
     9181 gtttgtgcgt ttcaatcatt tcataatggt aacgattatt atatatgtga tttcccctgc
     9241 aaacaagccg gtccgccgcc ccggcgttcc cacttatccg gctttgcctt ataattgctt
     9301 tttttatgta acagattcac ctatgaattt ccccaaaaca gcggcctccc tgctgctgct
     9361 tctcgcctcc ctcgccgcac acgcgctcga taccggccgc attccgcaaa acgaaatcgc
     9421 tgtatatgtc caagagcttg acagcggaaa agtcatcatt gaccaccgtg ccggcatacc
     9481 cgtcaatccc gcgtccacga tgaagctcgt taccgcgttt gccgccttca aaaccttcgg
     9541 cagcaattac cgctgggcga ccgagtttaa aagcaacggt acggtaaacg acggcacgct
     9601 tgacggaaac ctgtattggg cgggcagcgg cgaccccgtt ttcaatcagg aaaacctgct
     9661 tgccgtccaa cgccagttgc gcgacaaagg catccgcaat atcacggggc gcctgatgct
     9721 cgaccacagc ctgtggggcg aagtcggcag tcccgaccat tttgaagccg acagcggttc
     9781 gccgtttatg acgcccccaa atccgactat gctgtctgcc ggtatggtta tggtgcgcgc
     9841 cgaacgcaat gccgccggca gtaccgacat cctcaccgat ccgcctttgc cgcatatttt
     9901 tgcccaaaac aacttgaaaa ttaccgcctc ccaagctgcc tgcccttcgg tcaaaaaact
     9961 gatgcgcgca tctttttcgg gcaatacgct gaaattgcgc ggcaatattc ccgaaagctg
    10021 tttgggcaag cctgtcggtg tccggatgtt cgcgcttgac gaactgatcc gccaaagttt
    10081 taccaaccgc tggctgctcg gcggcggacg gatttcagac ggcatcggca tagccgacac
    10141 accggaaggc gcgcagacgc ttgccgttgc acacgccaaa ccgatgaagg aaattttgac
    10201 ggacatgaac aagcgttcgg acaatctgat tgcgcgttcc gtcttcctca aactcggcgg
    10261 cgacggcaaa ctgcccgccg tttccgaaca ggcggcgtct gccgtccggc gagaacttgc
    10321 cgtatcgggc atcgatgttg cggatttggt tttggaaaac ggttcgggtc tgtccagaaa
    10381 agaaagggta acggcgagaa tgatggcgca aatgttggaa acggcttatt tcagcccgtt
    10441 tgcacaagat ttcatcgaca cgctgcccat cgccggcaca gacgggactt tacgcaaccg
    10501 cttcaaacaa agcggcgggc tgttgcgctt aaaaaccggc acgctcaaca atgtccgcgc
    10561 ccttgcaggt tattggctgg gcgacaaacc gatggcggtg gtcgtcatca tcaacagcgg
    10621 ccgcgccgtt tccctgctgc ccgacttgga caacttcgtt gccaaaaaca tcatctccgg
    10681 cggcgacggc tggctggatg cgaaactgat gtgcaaagaa cgccgcgcct gaatcgggaa
    10741 aatataaaaa tgccgcctga actgttcgga cggcattttt gattttcaaa ccggaattac
    10801 agccccgctg ccgctttcaa tgaggccgct ttgtcggtgc gctcccaagt aaactcgggt
    10861 tcttcgcggc cgaaatgtcc gtaggcggca gattttccgt aaatcgggcg caagaggtcg
    10921 agcatttgga cgatgccttt gggacgcagg tcgaaatgtt cgcaaaccag cgcaatcagt
    10981 ttttcctcgc tgattttacc cgtaccgaaa gtatcgatgg aaatcgaagt cggttcggca
    11041 acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaagcctgc ggcgacgatg
    11101 tttttcgcca cataacggca ggcgtaggcg gcggaacggt ccactttgga cgggtctttg
    11161 ccggagaatg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac aatgattttg
    11221 cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg
    11281 atcaggtatt ttgtttcgtc ggtcagcagt tcgggcggca aaacgggctt gataatctgc
    11341 tcaatcacgg ctttactcag ttcttcatgg ctgatggcag gatcgtgctg ggtggacagg
    11401 acgacggtat caatgcgttt caccttgccg gtttcgctgt cgtaaaccac ggtcagttgg
    11461 gcttttgcgt cagggcgcag ccaaggcagg cggccgtctt tgcgcaattc gctttgacgc
    11521 tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag
    11581 gcgtagccga acatcaagcc ttggtcgccc gcgccttggt tcaggtcgat gccttcgcct
    11641 tcgttcacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag
    11701 ccgttggcgt caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt
    11761 gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcccgccaa tacgcacaag
    11821 cctgtgttga ccaaagtttc cgccgcgaca cgcgctttgg ggtcttgcgc caagatggca
    11881 tccaaaatcg catcggatac ttggtcggct actttgtccg gatggccttc ggaaaccgat
    11941 tcggaagtaa acagatattc gctcattgaa attttccttg aaaaacaaac catcttcttc
    12001 agacagcatg ttgtatgaac ataatgtcga cagcgggaaa tatagcaaaa tttccctatt
    12061 cgtgccattc agttgagaaa tattcctatt taaacggcgc tttggaatct ctgtccgtac
    12121 gtttcgtaca ggcaaaaaaa ttcccgcatc aagcgggttt ggattgctcc ggcgagccac
    12181 atcggttttt caaccgtcca ccttaccttt ccgtttgaaa agcaggttgg catggaattc
    12241 ccaactctta atgcagcgcg catcgtagca gaaaaggcat attgccgcaa tacttccctt
    12301 tttcagacgg catgtttcgt ttacaattca ggctgtttcc ccctttgcga accgccatgc
    12361 acatcctgtt gaccgccctg ctcaaatgcc tctccctgct gtcgctttcc tgtctgcaca
    12421 cgctgggaaa ccggctcgga catctggcgt tttacctttt aaaggaagac cgcgcgcgca
    12481 tcgtcgccaa tatgcggcag gcgggtttga accccgacac gcagacggtc aaagccgttt
    12541 ttgcggaaac ggcaaaatgc ggtttggaac ttgcccccgc gtttttcaaa aaaccggaag
    12601 acatcgaaac aatgttcaaa gcggtacacg gctgggaaca cgtgcagcag gctttggaca
    12661 agggcgaagg gctgctgttc atcacgccgc acatcggcag ctacgatttg ggcggacgct
    12721 acatcagcca gcagcttccg ttccacctga ccgccatgta caagccgccg aaaatcaaag
    12781 cgatagacaa aatcatgcag gcgggcaggg tgcgcggcaa aggcaaaacc gcgcccaccg
    12841 gcatacaagg ggtcaaacaa atcatcaagg ccctgcgcgc gggcgaggca accatcatcc
    12901 tgcccgacca cgtcccttct ccgcaggaag gcggcggcgt gtgggcggat tttttcggca
    12961 aacctgcata caccatgaca ctggcggcaa aattggcaca cgtcaaaggc gtgaaaaccc
    13021 tgtttttctg ctgcgaacgc ctgcccgacg gacaaggctt cgtgttgcac atccgccccg
    13081 tccaagggga attgaacggc aacaaagccc acgatgccgc cgtgttcaac cgcaataccg
    13141 aatattggat acgccgtttt ccgacgcagt atctgtttat gtacaaccgc tataaaacgc
    13201 cgtaacgaaa ataaaaatgc cgtctgaaca ggtttcagac ggcatcttgt catttgacga
    13261 tttcggacaa cgaccaacgc ggacggacgt tgaacgcacc gacctccctg cccttgccca
    13321 ggtgcatcgc accggcaaag gcaatcatgg caccgttgtc cgtgcagtat gccatcggcg
    13381 ggaaaaacac gctgactttt tcggacggat gtttcggctt gcctttgggg gtcgggattt
    13441 gtaccgtcat gttgccgaaa gtttcacgga gtttgcggtt tgcaccgacc ccgccggcga
    13501 ccactacggt tctgaaccct gtctgcaaca gggctttttt cacttttgcc tccaacactt
    13561 ctactaccgc atcttgaaac gcacggcaaa tattgttgcg ggtttgctcg ggtatggttt
    13621 ccgaaccgtt tgcctcacgc actttttcaa cggcagtcaa aacagcggtt ttcaatcccg
    13681 aaaaactcat ctgcaaatcg tcggaatgaa tcatcgggcg cggaaaaaca aacgcttcgg
    13741 gcctgcccga ttccgcaagt tccgacagtt tcgcaccgcc cggatacggc aaacccagca
    13801 gtttcgccgt tttgtcgaat gcctcgcccg ccgcatcatc gacgctctcg cccaaaagct
    13861 cgtaatcgcc tatgcccctg accgccataa tctgcgtatg cccgcccgaa accaacagcg
    13921 cgacaaaagg aaagtcgggt ttttcctccg ccaacagcgg cgacagcaga tgtccttcca
    13981 aatgatggac ggggatgacg ggcttatcca acgctaaagc cagcgcgttg gcgtagctcg
    14041 aacccgccag cagcgcaccg cccaaaccgg gcccctgcgt aaaggcaacc gcgtcaatat
    14101 cgccatacga tgcgcctgcc tgcgccaaac agccttcagt caacggaaca aggcggcgga
    14161 tatggtcgcg gctcgccaat tccggcacga ccccgccgta ttccgcgtgc attgccattt
    14221 gagtgtgcag gcagtgcgac cgcaatcctc gttcggtgtc gtacagcgca acgccggttt
    14281 cgtcgcaaga agattcgatt cctaatacca acatggtctg atgccgttaa aaactgaaaa
    14341 acgtattcta gcgaatttcg gcacgactgc cgtatcccca aaacggaaca tgccgtctga
    14401 agcccttcag acggcatcgt cgcaccgtat caaagcgttc cgtaagaatg cagcccgctc
    14461 aaaaacatat tcacgccgat aaaggcgaat gcggttacga acaaaccgat aatcgcccac
    14521 cacgccagca ctttgccgcg ccagcccgcc accagccgca aatgcagcca cacggcgtaa
    14581 ttcaaccaaa cgatgaacgc ccaagtctct ttcggatccc agctccaata gcgtccccaa
    14641 gcgtccgccg cccacaacgc gcccaaaatg gtggcaatgg taaagaacaa aaagccgacg
    14701 gcaatcgcct tatacatcac ctcgtcgatc aatgccgacg gcggcagcca cagttttccg
    14761 cctttttctt ccgcacgcag ggaaaccagt tcggcaatac cgagcatcgc ggaaatgcag
    14821 aacgcgccgt aaccgataaa gtttgccgga acgtggattt tcatccacca ggactggagc
    14881 gcgggaatca gcggctggat ggtatgcgcc tcacgcgaca cgctgtacca caacacaaag
    14941 ccgaccacga ccgccataaa gccgaacacg aagccgccca atttctgcac ggcaaattta
    15001 ccctcgtaat acaaatacat cagcgcggta atgaccaaaa acagaatgaa cacttcataa
    15061 aggttggaca cggggatatg ccctgcatcg ggacggagca gatagctttc gtgccaacgt
    15121 accagcagac cggtaaagcc tgctacggca gacacccatg caaacacggt tcccataccc
    15181 aacagcgtgt tggtcggcac attttttacg cttgccaaaa ccgcgcccga aatataggcg
    15241 aacagggcga agaagacaaa agcacactgc cacatgatcg ccgattggct gctgaggaaa
    15301 taccgcaaca ggaaaatctc tgccgattta atgttgccgc catacagacc gacggcggca
    15361 taggcaagca atacgcttaa aggaacaaac cagcgcatcg gtttgaaaaa ccaacccaaa
    15421 aacacggcaa taccggcact cgcccacaac atgaccgttt cgtaaatgtc catatgcata
    15481 ccggaacggg tctgcacgaa aaccgtagcc gcaaaaacca gcacggcaaa tacccaatcc
    15541 caaagattca gattgcggat caaagatttc tgaatcagca gctcgtgttc cggaagggtt
    15601 ttatagtgtt cagtcatgat tcaagtcctt gccgagccgt tgcaggctct cgacgtgttt
    15661 tggaaattcc ttctgcaaat cccgttcgct gcgggccgaa gacatagcaa aacggatttt
    15721 gccgtctgaa aacaataccc acgcccgttt ttcgcgcaca taaaacataa ataccgtacc
    15781 caaaaccaac aataccgagc cgagatagac caaaagcgca cccggcgaac gggtcatctg
    15841 caaacctgag gaacgcacct cggaaaaccc gtcaagctgg agcagcatgg gcgcgggata
    15901 ttccgtcagc cccgtatagg catccatact gtgcagcagg aaacggttcc gcgcttcatc
    15961 ctgccgccat tcgggcaagc cgtaccggcg tatggtttca tccaaagcag cgttcatcac
    16021 gccgtaaagc atttcgtaga aatagccctg catcttatcc tgctgccctt tcgggatatt
    16081 ggacgtaata aattcgtcca atcccaaata gcctttttgc gcaaagatat tcagcgtgtt
    16141 ttccgcagcc agcatgaatt gttcgcggat ttcggcaggt gcgtctttgg ttgcgtcggc
    16201 aaccagacgt ttgcgccctt ccccatcttt caaaaactca cgcaatgcca taaaggtgtc
    16261 cgctttcaac tgcttgtcca aggggatacg cagccagcgg tattgctgct gcaagccgct
    16321 gcgcgtgccg gtcagccaaa aataatcttt gtcctgcaaa atcggcagca tatagttttt
    16381 atattcgacc gcctgccctg ccgcatcacg gatgcggtac acgatggaag ggccgatatt
    16441 ggtgtatttt ttaccttcct gagtaacggc gcggacatcg ttcagagtgg atttcaggct
    16501 tttttcccgt tccgcaccct cgctcatgtc ctccacattc atagaagtga actgatcgaa
    16561 ctcaagacga tatttgtgtt tgccgatttc caacggaaac tggtgtatgg aggttgcctt
    16621 caacacgaca ggttcgcgcg aagcatccct caaattccac gccttgaatg tcaaatccga
    16681 accgccgtcg gcaaaactcg cctgataaat cgtgatgccg tgcaaggtca aaggatggtt
    16741 cacgcggatg gtgcgctcga gtttctcacc ggttgccttg tccgttactt caatatcgct
    16801 ggcaaaatcg cgcggcatac ccgtattgta aaaatcgata tggaattttt tcagtttgac
    16861 ttcaaaaggc aagtcctgaa ccaacatccc gttgtcggca ttcaggaaaa ccacatccgc
    16921 actttgcccc tcggaaatat tgacgttgcc cctaaatgag agattggacg cacccaaaat
    16981 actttcgggc ttgaaatcct tggcataaac cgcctgattg tccggaacaa tccgaccggc
    17041 cagcataccc agcttcagca gcaggttact gtctatcaac ccgcccaggc aaatgacaat
    17101 caaagcaaca tgggcaaaga tatagcccca tttgttcatc gtgccttttt tggcggcaat
    17161 cagaaccgac ccgtcctcac ggctgacggt ttttccctga aaaccccgca cctccagata
    17221 acgtttggca acttcggggg caatttttac atccaacagc gaagaatggc gcatcgccgc
    17281 cagagatttt tctttaacct tttcccggaa agacttcatt tcgcgccaaa acggcggaac
    17341 gttacggatt aaacacaaac tggtagaaac caccagaaac atcatgataa cgacaaacca
    17401 tgccgaagca tagacatcgt acaaacccaa aaaatcaaaa atccgagtcc aaaacggtcc
    17461 gaatttgacc aaataatccg tctgcggctg gttttgctgt aacaccgtgc cgataaccga
    17521 tgcaataccc agcagactga gcaaagcgac cgcaaagcgc atggagctga aaaaagcgaa
    17581 ccacggacgg gaaagaagtg tgggggatat acgggattta ctcattgtgt gtttattccg
    17641 ccatcaggaa tatgggaaag cagaattggg cagacagaaa acaacgtccc gattctactg
    17701 tcttgatgct ttttatttca agacaatgaa gacagcctgc atcgattcca acggttgcga
    17761 ttgaaaaaac ttatcgcaaa attttcagaa gccgtctgaa aaccttttca gacggcttct
    17821 gaagcagacc cttggatgat taacgcaaac cctggataaa gttagcaact gcttttaaat
    17881 cttcttcgga catacggttt gcaatatctt ccatgatggt atttttacgc tgaccggact
    17941 tgtaggcatt catctgttca acaatatatg cctggtgctg gccgcccaaa cgcggataag
    18001 cctgaatttc gcttccgcct cccggcatac ccgcaccgct cggaccgtgg caggacatac
    18061 acgccggcac ttttttatcg ctcaaaccgc cgcgatagat tttcgcgccc aattcgggat
    18121 tttccttagg attggcttcg ccggatttgg gctgctgttt ggcatagaat gcggatacgt
    18181 tcaaaatatc ctgatcgctc aaattcatta ccaccggttt catcacagct gccgaaccgt
    18241 gggtgcgttt gccgtcgcgg atgccgatag tttgatgata gatgtaagca gtatgctgtg
    18301 ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc tgctgcatgg caagccgcac
    18361 aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc tttgggagat gcggaaaccg
    18421 caccggcagc caaaacaaag gccaataaag tcaatcgtct catggagtgc tcctgattac
    18481 agcattggat aacgcaacaa tgctcttttt tatattcaaa tatgggattt ttgacccgat
    18541 taaaaccgat gattctgtaa acgtgttatt ctatactaaa tttacattaa attaccactg
    18601 tgtttcacat aaaaccaacc gcatattttt gctgtcggac aaacggcggc ggaaaacaag
    18661 gatatgccca tgaacctttt tcaaaacgcc aaattcttca cgacggtaaa ccatctcaaa
    18721 gacctgccgg acacaccgct cgaaattgcc tttgtcggca ggagcaatgc cggaaaatcc
    18781 agtgccatca ataccctgac caaccatgtc cgtcttgcct acgtttcaaa aacaccggga
    18841 cggacacagc atatcaactt cttcgagctg cagaacggca attttatggt cgatttgccc
    18901 ggctacggtt atgcccaagt ccccgaagcg gtacgcgcac attgggtcaa tctgctcggc
    18961 gactacctcc gccatcggaa acagcttatc gggctggttt tgattatgga tgcccgccat
    19021 cctttaaaag aactcgacat ccgtatgctg gactttttcc acacgaccgg cagaccggtt
    19081 cacatcctgc tgtcaaaagc cgacaaatta tccaaaaacg aacagataaa aaccctgtcc
    19141 caagtcaaaa aactgctcaa accttattcc gacaggcaaa acatcagcgt acagctgttt
    19201 tccagcctga aaaaacaagg tattgacgag gccaaccgaa ctgtcggaag ctggtttgac
    19261 gcagcagatg ccgccgcttc ctctccagag gaaaactgac ctcaattata cggaaaccgt
    19321 atccccccac ttgaccggcc gcaaacattt aaaaaattgc cactgccaaa tctaaaatgc
    19381 cgtctgaaag acttttcaga cggcattttg cggagtcttt aaaacaggga atccaactgc
    19441 tgctgtttgg aatccgtatt gctcggaagc accggcgttt cctgtacgtc ctgacgcgtt
    19501 tcgtcagacc ttccctgctg ttcgttttct actgccgctt catcatcttc ttttgcccgt
    19561 cgggaaggtt gcggcgcaat accgctgttg tccagcatca agcccggatc ggttaccata
    19621 cgttccttca tatagtattc gccattgctg ctgaccacac cttcaggcat tttcatccct
    19681 ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca cccaaaccgg caccgcaatc
    19741 gtaccgccgt agccggcacg ccccatactc ttaggtttgt cgaagccgat atatacggca
    19801 gtaaccacat cagggttaaa accgacaaac cacgcatctt tattgtcgtt ggtcgtaccc
    19861 gttttaccgg caatatccgt tcttcccaac gcagctgccc cccttgccgt accgacacgg
    19921 accacatcct gcataatctt atacataata taggcattgc gcggatcgat tgcctgaggc
    19981 gcattttgcc ctgccaccaa aggttgcatt tgggcgcgca acctgccgtc tctgtcataa
    20041 atcttatcga tcacgtgcga agaaacccta tatccgccgt tcgcaaatac actatatgcc
    20101 tccgccactt tcaacggcgt cgtctcgccc gtacctaaag ccatagacag gcttgccggc
    20161 agctcggacg gcctgaagcc gaaacgccgg atatactgtt gcgcgtaacc gacaccgata
    20221 gacatcaaaa tacggatgga aaccatattc ttggaagccg tcagagcctg tctcaaagta
    20281 atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc aaaccgaacc gttcggccct
    20341 ttccccggca gggaaatcgg cgcatcgtta accactgtgg acgcggtcat ccccttagat
    20401 aatgccgccg aatagacaaa cggcttaaag gtcgaacccg gctgccgcat tgcctgaacg
    20461 gcacgattga atgttttgct gtgaaaatca taaccgccga ccagcgcgcg cacagctccg
    20521 gtttttgcat ccagcgaaac caaagccccc tgcggcaacg gctcttgaac caccgcccaa
    20581 cgcccgccgt tgtttttgac acggatgacc gcgcccctgc ggatacggtc ctcccccatt
    20641 ttctcattat cgaccgctcg ggccgcaaaa cccaaggcgc gcctgtcaag cgcaacccgc
    20701 ctgccgccgg gcagctgtat gacgacattt ttctttttag taacatccaa cacaacggcg
    20761 ggaaccattt tatcgacggt atagagtccc gacagatact ggctgacagt ctcctcgaca
    20821 tcttcactct tactcaaatc gatatagttt tccgcaccgc ggtagctgct gccgcgatcg
    20881 aaattccgta gagccttgcg caatgcctcg gttgccgcct tctgatgatc ggtgcggacc
    20941 gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac catatttctc atacagttcc
    21001 cgacgcacca tttccgccac atataaagca ctctgatcga ttttccgaac aaaccgctca
    21061 taatgcagtt cctcattcaa tgcctgatcg cgctgttgca cggtaatcat cttctcctcg
    21121 agcatattgt tcaaaatata cttctggcgc aacttggcac gctccggatt aacaatcgga
    21181 ttataggcag acggagcctt gggcagtccc gcaagcatgg cggcttccgc caaagtcaaa
    21241 tctcggacat tcttattgaa atagatttgc gcggcagatg caaaaccata ggcgcgctga
    21301 ccgaggtaaa tctgattgaa atacaactca aggattttgt ctttgcttaa agactgctcg
    21361 attttatagg caagcaacac ctcattgaat ttgcgtgtga acgttttttc actgctcaaa
    21421 taaaaatttt tcgccacctg ctgtgtaatc gtactcgcac ccgactgcac gctgccggac
    21481 acgacattgc cgacggcagc gcgggcaaca ccccaaacat ccacccccca atgccggtaa
    21541 aagcgtttat cctcggcggc aataaccgca ttccgcaaca cctcggggaa atcgccgatt
    21601 tttgtaaatt cgcgccgctg ctccccatac ataccgatga cttctccatc cgccgaataa
    21661 atagtcaacg gcattttagg ctggtaatgc tgcaaagaat ccaaagacgg cagtttcgga
    21721 tacgttacca aaatagcaat ggcaaccaga cccactccaa atacacaaaa accaaaaaac
    21781 aaaccaaaac aagtagttaa aatcttttta atcatagctg aataataatt taccattatt
    21841 ggtattaaat aaagtaaaat agcaaccgat ttctacaaag cacggtttca atgtgcaaag
    21901 aacaaggaat ccattacgga taccgaaacg gttactcact gtacaaataa agcaggaact
    21961 ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa aaacagatgc caagctccct
    22021 aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca tcgatatcga ccagcattcc
    22081 atcaaaatgg tacaattgtc aggacgtagt ttaaaccaaa ttcaattgga aaaatacgtc
    22141 attgccaaat taccaaagaa tatcattcaa ggcaataaag tccaaaatta cgatcaactt
    22201 gttacatatt tgcaacaagc ctatgccaaa ctgggtactt cgtgcaaaaa catcgtcgcg
    22261 tccgtcccgc aaaatctggc aaccatcgaa caattgacct acacggccaa agatgcagaa
    22321 ttggacctgc aggggttcgt ggagtcctcc atctccgaag ccagctcgat atcgctcgaa
    22381 gaagccaatt acgactatca ggtcttgtcc caatcggccg tcggcgaagc tgttttgtcc
    22441 gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg cattcaacgc agccggtatg
    22501 aaattatccg cgcttgatgt ggacattttc ggacaataca acgcctacgc gctgtggata
    22561 aaccattttg cccccgagct tgcggacgaa aaagtggcca ttttcggcgt atatgccgca
    22621 cagacctacg ccctggtcat ccaagacgga aaaatcctat acaaacagga aacctccgtc
    22681 agcgcagaac agctcaacca actcatccag cgcacctatc aggtaacagc agaaaaagcg
    22741 gaagagatca tcaactcccc gcaaaaacct tccgattacc aagaaagcgt ggcaaactat
    22801 ttcaaccagc agattaccca agaaatacaa agggtcttgc agttttatta caccacgcag
    22861 accgcagacg atatgaccga catcaagcat atcctgctga cgggggaagc ggtgcgccag
    22921 aaaggcatcg cccaaaccgt cgcctcgcaa accaatgcgg atgtacaatg cgtccatccc
    22981 gcgcgttatt ttgcgaacga cctcaaaaca gacgaacaac aattcgaact tgatgcgccg
    23041 acactgacca aggcgttcgg tttggcggta cggggattat aattatgaac aatttaatca
    23101 aaatcaacct cctcccctac agggaagaga tgaacaaacg caaacagcag cagtttaaaa
    23161 cgctgatgta cggcgccgtg ctgacgggcg ttgccgccgt tgcggcaacc tacctgttta
    23221 tcgacaatat gatcaataaa cagtcggaaa aaaacacgct gctggaaatc tccatcgcac
    23281 acttggatac cgagctgtcg gaaatacaaa agctcaaaca ggaaaaagat gccttcctga
    23341 ttaagaaaaa caaaatcggg gagctccaga tcaaacgcct ccaagccgca aaaatcctcg
    23401 acagcctgaa tgaggccgtc cccggaagca cctacctaac ctcgctggat gccgttaccg
    23461 ccgactctta tcggctcagc ggcaggacat ccagcgacaa ccgcgttgcc gccatgatga
    23521 gggcgatgcc caataccggc atattcaagc aacccgaatt gttaagcatc aagaaaaaca
    23581 attcgcatca agaatttacc cttcaggcaa cattacaacc tatcgtaaag gcggccgaat
    23641 ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa ctgaatggct tctaaatcat
    23701 ctaaaaccaa cttggatctc aacaaccttc acctgctcaa ccttcctgcc aggcttttta
    23761 tcgccctgct ggtcgttgcc gccgtgctgg ggctcggtta tgccggattg ttcaaaagcc
    23821 agatggaatc ccttgaggaa tatgaagcaa aagaaaccga actgaaaaac acctacaaac
    23881 agaaaagtat cgacgcggcc agcctgaaca accttaggga cgaacttgcc tcaatccgct
    23941 ctgccttcga tatcatgttg aaacagctgc cgacagatgc agaaattccc aatttggttc
    24001 aagagcttca tcaggcgggt tcgagcaacg gtctgcgctt ggacagcgtt atgccccaac
    24061 ctcccgtaga tgacggtccc atcaaaaaat taccctattc catttccatt accggaaatt
    24121 acgaacagat cagccaattt acccgcgatg tcggcagtct ctcccgaatc attacccttg
    24181 agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa tcctgacggc aagagcagta
    24241 tcctgaacct cagcgccatt gccaccacct accaagcaaa atccgtagaa gagcttgccg
    24301 cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt agggaaacca tgaaacacta
    24361 tgccttactc atcagctttc tggctctctc cgcgtgttcc caaaattctg aagacctaaa
    24421 cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa atcatacctt tccaagcacc
    24481 taccctgccg gttgcgccgg tatacagccc gccgcagctt acagggccga acgcattcga
    24541 cttccgccgc atggaaaccg ccaaaaaagg ggaaaatgcc cccgacacca agcgtattaa
    24601 agaaacgctg gaaaaattca gtttggaaaa tatgcgttat gtcggcattt tgaagtccgg
    24661 acagaaagtc tccggcttca tcgaggctga aggttatgtc tacactgtcg gtgtcggcaa
    24721 ctatttggga caaaactacg gtagaatcga aagcattacc gacgacagca tcatcctgaa
    24781 cgagctgata gaagacagca cgggcaactg ggtttcccgt aaagcagaac tgctgttgaa
    24841 ttcttccgac aaaaacaccg aacaagcggc agcacctgcc gcagaacaaa attaagaaga
    24901 ggattactcc attatgaata ccaaactgac aaaaatcatt tccggtctct ttgtcgcaac
    24961 cgccgccttt cagacggcat cggcaggaaa cattacagac atcaaagttt cctccctgcc
    25021 caacaaacag aaaatcgtca aagtcagctt tgacaaagag attgtcaacc cgaccggctt
    25081 cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa caaaccggca tttccatgga
    25141 tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa atcagtgccg cacaaaacag
    25201 cagccgtgcg cgtctggttc tgaatttgaa caaaccgggc caatacaata ccgaagtacg
    25261 cgggaacaaa gtttggatat tcattaacga atcggacgat accgtgtccg cccccgcccg
    25321 cccagccgta aaagccgcgc ctgccgcacc ggcaaaacaa caggctgccg caccgtttac
    25381 cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg cagcggcatc
    25441 ggcaaaacaa caggcggcga caccggcaaa acaaaccaat atcgatttcc gcaaagacgg
    25501 caaaaatgcc ggcattatcg aattggcggc attgggcttt gccgggcagc ccgacatcag
    25561 ccaacagcac gaccacatca tcgttacgct gaaaaaccat accctgccga ccgcgctcca
    25621 acgcagtttg gatgtggcag acttcaaaac accggttcaa aaggttacgc tgaaacgcct
    25681 caataacgac acccagctga ttatcacaac aaccggcaac tgggaactcg tcaacaaatc
    25741 cgccgcgccc ggatacttta ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg
    25801 cggcgtgaac aatgcgccca aaaccttcac aggccggaaa atctcccttg acttccaaga
    25861 tgtcgaaatc cgcaccatcc tgcagatttt ggcaaaagaa tccgggatga acattgttgc
    25921 cagcgactcc gtcaacggca aaatgaccct ctccctcaaa gatgtgcctt gggatcaggc
    25981 tttggatttg gttatgcagg cgcgcaacct cgatatgcgc cagcaaggga acatcgtcaa
    26041 catcgcgccc cgcgacgagc tgcttgccaa agacaaagcc ttcttacaag cggaaaaaga
    26101 cattgccgat ctgggcgcgc tgtattccca aaacttccaa ttgaaataca aaaatgtgga
    26161 agaattccgc agcatcctgc gtttggacaa tgccgacacg accggaaacc gcaacacgct
    26221 tgtcagcggc aggggcagcg tgctgatcga tcccgccacc aacaccctga ttgttaccga
    26281 tacccgcagc gtcatcgaaa aattccgcaa actgattgac gaattggacg tacccgcgca
    26341 acaagtgatg attgaggcgc gtatcgtcga agcggcagac ggcttctcgc gcgatttggg
    26401 cgttaagttc ggcgcgacag gcaggaaaaa actgaaaaat gagacgagcg cattcggctg
    26461 gggcgtgaac tccggcttcg ggggcggcga taaatgggag gcccaaacca aaatcaacct
    26521 gccggttgcc gctgccgcaa acagcatttc gctggtgcgc gcgatttcct ccggcgcgtt
    26581 gaatttggaa ttgtccgcat ccgagtcgct ttcaaaaacc aaaacgcttg ccaatccgcg
    26641 cgtgctgacc caaaaccgca aagaggccaa aatcgaatcc ggttacgaaa ttccttttac
    26701 cgtaactaca gcctcgggcg gcggcaactc taccaacacg gaactcaaaa aagccgtctt
    26761 ggggctgacc gttacgccga acatcacgcc cgacggacaa atcatcatga ccgtcaaaat
    26821 caacaaagac tcgcctgcac aatgtgcttc aggcaacaac acaatcctat gtatttcgac
    26881 caaaagcctg aatacgcagg ctatggttga aaacggcggc actttgattg tcggcggtat
    26941 ttatgaagaa aacaacggca atacgctgac caaagtcccc ctgttgggcg acatccccgt
    27001 tatcggcaac ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt
    27061 cattaccccg aggattatag atacggccgg caacagcctg cgctattgat gcgtcaaaat
    27121 aagggcatat gttttacagc atatgccctt tctttatgct ttttgccgcg accgaaatgc
    27181 cgtcattccc gcgcaggcgg gaatccaact tgtccggttt cggttgtttt tcgtctcgta
    27241 acttttgagc cgtcattccc gcgaaatcgg aaatccagtc cgttcagttt cggtcatttc
    27301 cgataaattc ctgttgcttt tcatttctag attcccactt ttgtgggaat gacggcggaa
    27361 gggataaatc ctcgcaatcc aaagcctcgt catttccaca aaaaacagca acccgaaaca
    27421 ccccgtcatt cccgagcagg cgggaatcta ggtctttaaa cttcggtttt tttccgataa
    27481 attcttgcct cattgaaatt ctagattccc gctttcgcgg gaatgacggg atatgggttt
    27541 ccgtgcggac gtgttcggat ttccgcctgc gcgggaatga cgattcgggt attactgacg
    27601 attcatcggg ccgtagggtg ggcttcagcc caccaatttc accgcatcaa gggttttggg
    27661 gaataggcgt aactcggcgg tttcggtttt tggcgaagtt ggttggaatc ggcgggctga
    27721 agcacaccct acagtccacc ctacaaccca tcttacgcat ctgccttacg catttatcct
    27781 tcgcctgccg atatgccgtc attcccgcac aggcgggaat ctagaaccgc aacgcggcag
    27841 gaatctgtcg gatacggctg aaaccgaacg gactggattc ccgcctgcgc gggaatgacg
    27901 gctgcagatg cccgacggtc tttatagcgg attaacaaaa atcaggacaa ggcggcgaag
    27961 ccgcagacag tacggatagt acggaaccga ttcgcccggt gcttcatcac cttagggaat
    28021 cgttctcttt gagccggggc ggggcaacgc cgtaccggtt tttgttcatc cgctatagta
    28081 ttgataaaca tattatcttc aatatattca attggataat tgtttaccta agcaaagata
    28141 attgcctttt cctgacaaat taagtgaaat caacggattg tcaaaacaca gcctgaaata
    28201 aaaaacctcc ctgatttctt ttatttttcc ttaaaatcag aaaagttcgg gatggtcggg
    28261 ttatttttcc aaacgtaccg ccgccctgcc gatttcgtat aaaatcccgc cgtaacccga
    28321 caagcccgaa ccctgccgcc ccgaaaggcg ggggggtcaa acattaagga accgtgatga
    28381 aaaactttaa cggcaaactc atcctcatcg gactgatggg cgcgggcaaa actacgctgg
    28441 gccggcaaat ggcgcagcgg ctggattacc gtttttacga cagcgatcac gaaatcgccg
    28501 cagcggcggg cgttcccatc cccaccatat ttgaaatgga aggcgaacag ggattccgtt
    28561 cgcgcgaaac cgccatactc aaaaaactga ttgtcctgcc gcatatcgtc ctgtccaccg
    28621 gcggcggcgc ggtgttaaaa gaagaaaacc gcgcccttat ccgcaaaagc ggcacggtcg
    28681 tttatctgca cgccccgccc gaaaccctgc tcgaacgcac gcgctgcgac aacagccgcc
    28741 ctttgctgca agttgccgat cctttggcga aattgcgcga actctacgcc gcacgcgacc
    28801 ccgtttaccg ccaaaccgcc gactttaccg tagaatcggc aaactgccgg gaaaccgtgc
    28861 aaaccctgct caaacgctta tcccgataaa ccggcatatg cgccgctccc cgaaaaccaa
    28921 accgcacccc gcccggcggg ccggcggttc aaactttaag gaacaacaat gaaaacactg
    28981 accgtacaca cgccttcgca cagctacccc atctttatcg gcaacgggct gctgccgcag
    29041 gcgggaagcc tgctcaaacc gcatttgggc aaacgcgccg ccatcatcac caacgaaacc
    29101 gtcgccccgc tctacctcgg cacgcttcag acggcattgg atgcggcagg cgtatcccat
    29161 ttcagcatca tcctgcccga cggcgaggcg cacaaaaact ggcagacgct caacctcatc
    29221 tttgacgggc tgatgcaaaa ccgcgccgaa cgtaaaacca cgctgattgc gctgggcggc
    29281 ggcgtgatcg gcgacatggt cggctttgcc gccgccacct accagcgcgg cgcgccgttc
    29341 atccaaatac cgaccacgct gttgagtcag gtcgactcat cggtgggcgg aaaaaccgcc
    29401 atcaaccacc cgctcggcaa aaatatgatc ggcgcgtttt accagccgca ggcggtgctt
    29461 gcagatttgg acacgctgca caccctgccc gcccgcgaac tgtccgccgg tatggcggaa
    29521 gtcatcaaat acggcgcgct cggcgacatc ggcttttttg aatggctgga acagcatatg
    29581 cccgaactga tggcactcga acgcgcgccc ctcacccaag ccgtgtaccg ctgctgccaa
    29641 atgaaggcag acatcgtcgc ccaagacgaa accgaacagg gcatacgcgc atggctcaac
    29701 ctcggacaca ccttcggaca cgccgtcgaa gcggaaatgg gttacggcgt atggctgcac
    29761 ggcgaggccg ttgccgccgg ctgcgtgttg gcggcgcgtt tgtccgaaca actgggcaaa
    29821 acctccgccg cagataccgc gcggctcgcc gccctgctcg aagccgccgg actgccgtcc
    29881 gcaccgcccg tgtttgcctt tgaaaaatgg ctggcacata tgagccacga caaaaaagtc
    29941 agcggcggca tcatgcgctt tatcggtctg aaccggctgg gcgaagccgt catcaccgaa
    30001 attaccgaca cggacatcct ccggcgcacc ctgcaaccgt atctctgatt tcctctgccg
    30061 atgtgttgcc gcgcgggttt gacgcacgat gatgactttc catcatcatt ctccgcgaaa
    30121 gcgggcatcc ggttattcca tattgcaggg atttatccgg aataacgaca acctgtccgc
    30181 cgtcatttcc gcgcagatgg aaatttggaa attcaaaatc acaggaattt atccggaata
    30241 tagcggatta acaaaaatca ggacaaggcg gcgaagccgc ag
//
LOCUS       DAXDZG010000018        30243 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXDZG010000018 DAXDZG010000000
VERSION     DAXDZG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.018"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(66..1208)
                     /locus_tag="ACFB78_000714"
     CDS             complement(66..1208)
                     /locus_tag="ACFB78_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC1268761.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1509..1772
                     /gene="rpsT"
                     /locus_tag="ACFB78_000715"
     CDS             1509..1772
                     /gene="rpsT"
                     /locus_tag="ACFB78_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC1268762.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1932..3080
                     /locus_tag="ACFB78_000716"
     CDS             1932..3080
                     /locus_tag="ACFB78_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC1268763.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3222..3923
                     /locus_tag="ACFB78_000717"
     CDS             3222..3923
                     /locus_tag="ACFB78_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC1268764.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            3981..5789
                     /gene="aspS"
                     /locus_tag="ACFB78_000718"
     CDS             3981..5789
                     /gene="aspS"
                     /locus_tag="ACFB78_000718"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC1268765.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5939..6223
                     /locus_tag="ACFB78_000719"
     CDS             5939..6223
                     /locus_tag="ACFB78_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC1268766.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6390..8282
                     /gene="speA"
                     /locus_tag="ACFB78_000720"
     CDS             6390..8282
                     /gene="speA"
                     /locus_tag="ACFB78_000720"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC1268767.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8381..9304
                     /gene="speB"
                     /locus_tag="ACFB78_000721"
     CDS             8381..9304
                     /gene="speB"
                     /locus_tag="ACFB78_000721"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC1268768.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9502..10968
                     /locus_tag="ACFB78_000722"
     CDS             9502..10968
                     /locus_tag="ACFB78_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC1268769.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11172..12248)
                     /locus_tag="ACFB78_000723"
     CDS             complement(11172..12248)
                     /locus_tag="ACFB78_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC1268770.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12312..12449
                     /locus_tag="ACFB78_000724"
     CDS             12312..12449
                     /locus_tag="ACFB78_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC1268771.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12471..13613
                     /gene="bioF"
                     /locus_tag="ACFB78_000725"
     CDS             12471..13613
                     /gene="bioF"
                     /locus_tag="ACFB78_000725"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC1268772.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13623..14270
                     /locus_tag="ACFB78_000726"
     CDS             13623..14270
                     /locus_tag="ACFB78_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC1268773.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14258..15040
                     /gene="bioC"
                     /locus_tag="ACFB78_000727"
     CDS             14258..15040
                     /gene="bioC"
                     /locus_tag="ACFB78_000727"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC1268774.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15282..16719
                     /locus_tag="ACFB78_000728"
                     /pseudo
     CDS             15282..16719
                     /locus_tag="ACFB78_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            16722..17820
                     /locus_tag="ACFB78_000729"
                     /pseudo
     CDS             16722..17820
                     /locus_tag="ACFB78_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17832..18367
                     /locus_tag="ACFB78_000730"
                     /pseudo
     CDS             17832..18367
                     /locus_tag="ACFB78_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            18369..19011
                     /locus_tag="ACFB78_000731"
                     /pseudo
     CDS             18369..19011
                     /locus_tag="ACFB78_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19032..19391
                     /locus_tag="ACFB78_000732"
     CDS             19032..19391
                     /locus_tag="ACFB78_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC1268775.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19483..19558
                     /locus_tag="ACFB78_000733"
     tRNA            19483..19558
                     /locus_tag="ACFB78_000733"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19516..19518,aa:Asn,seq:gtt)
     gene            complement(20056..20967)
                     /gene="crgA"
                     /locus_tag="ACFB78_000734"
     CDS             complement(20056..20967)
                     /gene="crgA"
                     /locus_tag="ACFB78_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC1268776.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            21329..21784
                     /locus_tag="ACFB78_000735"
     CDS             21329..21784
                     /locus_tag="ACFB78_000735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC1268777.1"
                     /translation="MIDAGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVF
                     TGGHGKLYQSDGRHRVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGV
                     DVLYMHFHKANEFIGLSRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(21992..23377)
                     /gene="pntB"
                     /locus_tag="ACFB78_000736"
     CDS             complement(21992..23377)
                     /gene="pntB"
                     /locus_tag="ACFB78_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC1268778.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(23457..23840)
                     /locus_tag="ACFB78_000737"
     CDS             complement(23457..23840)
                     /locus_tag="ACFB78_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC1268779.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(23844..25382)
                     /locus_tag="ACFB78_000738"
     CDS             complement(23844..25382)
                     /locus_tag="ACFB78_000738"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC1268780.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGGGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            25671..26504
                     /gene="serB"
                     /locus_tag="ACFB78_000739"
     CDS             25671..26504
                     /gene="serB"
                     /locus_tag="ACFB78_000739"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC1268781.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            26568..27791
                     /locus_tag="ACFB78_000740"
     CDS             26568..27791
                     /locus_tag="ACFB78_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC1268782.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFRRTAQKFPFIKRDNIKIIPLAVCMFVLIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMVLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            27952..29532
                     /gene="purH"
                     /locus_tag="ACFB78_000741"
     CDS             27952..29532
                     /gene="purH"
                     /locus_tag="ACFB78_000741"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC1268783.1"
                     /translation="MSVIKRALISLCDKAGAVEFAQTLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAEYDGMISNYLTSLSDGVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLAAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         7400 a         7370 c         7911 g         7562 t
ORIGIN      
        1 tctatttcca tccaatataa aatgccgtcc gaacatcgtt cggacggcat ttttaatgct
       61 tcgaatcagt tggtgccgac tttgattttc tgccacaggc tgaccgacag ttttttcgca
      121 tccgtgctca tttgcggcat gacgaaaccg tctttcatat cctgctcgtt cgggaagatg
      181 gaacgggtgt tcaccaactc ggcaggcatt ttttcgcgcg ccggtttgct ggcgggggcg
      241 aaggttacgg cgatgccgtt tttcgccgcg atttcggggt cgagcgtgta gttgatgtat
      301 ttgtgggcgt tggcgacgtt tttcgcatcg gcgggaatca gccaagactc aatccagaag
      361 cccatacctt tcggtgtcag cacttcgatg ccgacgttgt ttttcacttc ttcggaacgc
      421 gctttcgcca agttcaaatc gccgccgttg cctgccgcca ggcagatgtc gccgcgcgcc
      481 agctcgtcga tgatggacgg gctgaaacgt ttgacgtccg gacggataga cttcaacact
      541 tccgccgccg ccttcaaatc ttcaggattc gaacctttgg ggtctttgcc caagtagttc
      601 agcaaaatcg ggaacatttc gctcggggtg tcccacaagg cgatgccgca ggatttcagc
      661 ttgcgggtgt attcgggttt gaacagcaaa tcccagccgt tttcgggcag cttgccgccc
      721 aaaagctctt tgcccttcgc cgtaatcgca accgtgttca cgccggagaa ataggggacg
      781 gcatactggt tgcccgggtc ggcggtttcc agcattttca agagttcggg atcgatgttt
      841 ttatagttgg gaatcaggtc tttgttgact ttttgatacg cgcccgcctc gatttggcgc
      901 ggcaggaagg cgatgcccgg cacaaccaaa tcgtaaccgg atttgccggt cagcattttg
      961 gcttccagcg tttcgttgtt ttcgtacaag tcgtaagtca gcttcagatt gttggctttt
     1021 ttaaagtctt cgaccgtact ctcgtcaaca taattcgacc agttgtagat gttcagagta
     1081 tcggtggcag cggcttcggc attggcagca ggtgcgctgc ctgcttgagg ctgcacggcg
     1141 tttttttcgc tgccgccgca ggctgccaga gacagcgcgg ccaaaacggc taatacggat
     1201 tttttcatac gggcagattc ctgatgaaag aggttggaaa aaaagaaaac cccgcgcccc
     1261 ataaacaccc cggcgcaagg tttgggcatt gtaaagtaaa tttgtgcaaa ctcaaagtat
     1321 attggactga tttttttgaa aatttgacac ttttgcgccc aaaggcggag gatgtgccgg
     1381 attttgccgc ccgcgcgcgc cggtaaaacg gcttatggga aaataaaggg aaaaggcttg
     1441 catataatgc aaaccccctt taaaattgcg cgtttacaga atttattttt ctttcaggag
     1501 attccaatat ggcaaacagc gcacaagcac gcaaacgtgc ccgccagtcc gtcaaacaac
     1561 gcgcccacaa cgctagcctg cgtaccgcat tccgcaccgc agtgaaaaaa gtattgaaag
     1621 cagtcgaagc aggcgataaa gctgccgcac aagcggttta ccaagagtcc gtcaaagtca
     1681 tcgaccgcat cgccgacaaa ggcgtgtttc ataaaaacaa agcggctcgc cacaaaagcc
     1741 gtctgtctgc aaaagtaaaa gcactggctt gatttttgca aaaccgccaa ggcggttgat
     1801 acgcgataag cggaaaaccc tgaagcccga cggtttcggg gttttctgta tttcgggggt
     1861 aaagttcgaa atggcggaaa gggtgcggtt ttttatccga atccgctata aaatgccgtt
     1921 tgaaaaccaa tatgccgaca atgggggcgg agatgaatac acgaaatatg cgctatattc
     1981 ttttgacagg actgttgccg acggcatccg cttttggaga gaccgcgctg caatgcgccg
     2041 ctttgacgga caatgttacg cgtttggcgt gttacgacag gatttttgcg gcacagcttc
     2101 cgtcttcggc agggcaggaa gggcaggagt cgaaagccgt actcaatctg acggaaaccg
     2161 tccgcagcag cttggataag ggcgaggcgg tcattgttgt tgaaaaaggc ggggatgcgc
     2221 ttcctgccga cagtgcgggc gaaaccgccg atatctatac gcctttgagc ctgatgtacg
     2281 acttggacaa aaacgatttg cgcgggctgt tgggcgtacg cgaacacaat ccgatgtacc
     2341 ttatgccgtt ttggtataac aattcgccca actatgcccc gagttcgccg acgcgcggta
     2401 cgactgtaca ggaaaaattc ggacagcaga aacgtgcgga aaccaaattg caggtttcgt
     2461 tcaaaagcaa aattgccgaa aatttgttta aaacccgcgc ggatctgtgg ttcggctaca
     2521 cccaaagatc cgattggcag atttacaacc aaggcaggaa atccgcgccg ttccgcaata
     2581 cggattacaa acctgaaatt ttcctgaccc agcctgtgaa ggcggatttg ccgttcggcg
     2641 gcaggctgcg tatgctcggt gcgggttttg tccaccagtc caacggacag agccgtcccg
     2701 aatcgcgttc gtggaacagg atttatgcca tggcaggcat ggaatggggc aaattgacgg
     2761 tgattccgcg cgtgtgggtg cgtgcgttcg atcagagcgg cgataaaaac gacaatcccg
     2821 atattgccga ctatatgggg tatggcgacg tgaagctgca gtaccgcctg aacgacaggc
     2881 agaatgtgta ttccgtattg cgctacaacc ccaaaacggg ctacggcgcg attgaagccg
     2941 cctacacgtt tccgattaag ggcaaactca aaggcgtggt acgcggattc cacggttacg
     3001 gcgagagcct gatcgactac aaccacaagc agaacggtat cggtatcggg ttgatgttca
     3061 acgactggga cggcatctga ataccgtttc aaacggcata tcaagttgcc gtgccgtctg
     3121 aagccgccgg cggtttggcg gggcgggata aaaaattcgt ttgaatccgg gcaaacgcgt
     3181 ataatgtgcg gtttgtacgg actttgtcgg aatcaagcaa gatgacggaa cctgcggccg
     3241 aaggcggcaa agctgccaag gcgttaaaaa aatatctgat tacaggcatt ttggtctggc
     3301 tgccgattgc ggtaacggtt tgggtggttt cctatatcgt ttccgcgtcc gaccagcttg
     3361 tcaacctgct gccgaagcaa tggcggccgc aatatgtttt ggggtttaat atccccgggc
     3421 tcggcgttat tgttgccatt gccgtattgt ttgtaaccgg gttatttgcc gcaaacgtgt
     3481 tgggccggca gattcttgcc gcgtgggaca gcctgttggg tcggattccg gttgtcaaat
     3541 ccatctattc gagtgtgaaa aaagtatccg aatcgctgct gtccgacagc agccgttcgt
     3601 ttaaaacgcc ggtactcgtg ccgtttcccc aatcgggtat ttggacaatc gcattcgtgt
     3661 ccggtcaggt gtcgaatgcg gttaaggccg cattgccgca ggatggcgat tatctttccg
     3721 tgtatgtccc gaccacgccc aacccgaccg gcggttacta tattatggta aagaaaagcg
     3781 atgtgcgcga actcgatatg agcgtggacg aagcgttgaa atatgtgatt tcgctgggta
     3841 tggtcatccc tgacgacctg cccgtcaaaa cattggcagg acctatgccg cctgaaaagg
     3901 cggagttgcc cgaacaacaa taaagccgcc gttcagacgg cattttccgt tttcagttta
     3961 aatcaataaa aggtgatttt atgcgtacca actattgcgg cctgatcagc gagcaatact
     4021 taggccaaac cgttaccgtc aaaggctggg tacaccgccg gcgcgaccac ggcggtgtga
     4081 tttttatcga cctgcgcgac cgcgaaggca tcgtccaagt cgtgatcgat cccgacacgc
     4141 ccgaagcgtt tgccgctgcc gattccgccc gtaacgaata cgttttgggc attaccggcc
     4201 gcgtacgcaa ccgtcccgaa ggcacgacca acgacaaaat gatttccggc aaaatcgaga
     4261 ttcttgccaa agaaatcgaa gtgttgaacg ccgccgctac gccgccgttc caaatcgacg
     4321 acgaaaacat cagcgaaaac gtgcgcctga ccaaccgcgt tatcgacctg cgccgccctg
     4381 tgatgcagcg caacctgcgc ctgcgttacc aagtcgctat gggcgttcgc cgttatctgg
     4441 acgcacaagg cttcatcgac atcgaaacgc cgatgctgac ccgctccact ccggaaggcg
     4501 cgcgcgacta cctcgtaccg agccgcgttc atccgggcga gtttttcgcg ctgccgcaat
     4561 cgccgcaatt attcaaacaa ctgttgatgg tggcgggttt cgaccgttac taccaaatca
     4621 ccaagtgctt ccgtgacgaa gacttgcgcg ccgaccgcca acctgaattc actcaaatcg
     4681 acttggaaac ctcgttcctg aacgaggatg aaatcatgga catcactgaa ggcatggcca
     4741 aacaagtctt caaagatgct ttgaacgtag atttgggcga cttcccacgc atgccttatt
     4801 ctgaagccat gttctactac ggctctgaca aaccggacat gcgcatcaac ttaaaattca
     4861 ccgagttgac cgacctgatg aaaacggaag aattcaaagt cttccgtggc gcagccgaca
     4921 tgaaaggcgg ccgcgtagtc gctctgcgcg tgcctaacgg cgccaaattc agccgcaaag
     4981 aaatcgacga atacactaaa tttgtcggca tctacggcgc gaaaggtctg gcatacatca
     5041 aagtaaacga tgcgggcaac ctctccaacg gcgaagacag cggcctgcaa tctccaatcg
     5101 taaaattctt gtctgaaaac gccctgaaag aaatcatcga gcgtaccgcc gcgcaaaacg
     5161 gcgacatcat cttcttcggt gcggacaaag ccaaagtcgt gaacgaagcc gtcggcgcat
     5221 tgcgtatcaa agtcggcttg gaacacggca aagacaacgg ctacttcaca gacgaatgga
     5281 aacctttgtg ggttgtcgat ttcccgatgt tcgaatacga cgaagaagcc gaccgctacg
     5341 ttgccgtaca ccatccgttt accgctccta aagaaggtca tgaagacctg atggtttccg
     5401 atcctgcaaa ctgcctggcc cgtgcctacg atatggtatt gaacggctgg gaaatcggcg
     5461 gaggctctat ccgtatccac cgtgcagacg tacaagagaa agtgtttgcc gcgctgaaaa
     5521 tcagccctga agagcaacaa gagaaattcg gcttcctctt ggacaacctg aaattcggtg
     5581 cgcctcctca cggcggcctt gcattcggcc tcgaccgtct ggtaacgctg atgaccggtg
     5641 ccgaatccat ccgcgacgtg attgccttcc cgaaaacaca acgcgcccaa tgcctgctga
     5701 ccgatgcgcc caacagcgtg gacgataagc agttgcgtga attgagcctg cgtctgcgtc
     5761 aaaaagcagt tgaaacaaaa gaagcataag gaaaacagag ccgtttgacg gctctgtttt
     5821 ttcagacggc atttacgctt cttgacttcc ctctaattca aacctaattt tgctgtgttt
     5881 taatggcggt attgaaaaac acatcttgtt caatcaaccg ataaaaaaag gactgaaaat
     5941 gaaaaaactg ttattggccg ccgttgtttc cctaaatgcc gcaaccgcat ttgccggcga
     6001 ctctgccgag cgtcagattt acggcgatcc ccattttgaa caaaaccgca caaaagccgt
     6061 gaaaatgttg gaacagcgcg gttatcaggt ttacgatgtc gatgccgacg actactgggg
     6121 caaacctgtt ttggaagtgg aagcctataa agacggccgc gaatacgaca tcgtgttgtc
     6181 ttaccccgac ctgaaaatca tcaaagagca gctcgatcgc tgactccttt gatggaaaga
     6241 tgaaccaaaa tgccgtctga agcgttcaga cggcattttg cctgttcctc atcaggtatg
     6301 aggcaggctt ttcttattaa aaaaatgaca tttcacgctg atttgttata atcattcctt
     6361 ttcaacacga cagacggagc aggtttatta tgcctatcct taccatccgt gaagtgtgca
     6421 acattaatca ttggagcata ggttattatg atgttgacga ttccggcgaa atcatcgtcc
     6481 gccccaatcc ctcgcaacac aatcaaactg tttcactgca aaaactgact gaagccgtgc
     6541 aacaaaaaca tcaggcgcgc ctgcctgttt tgttttgttt tccgcaaatc ctcgaacacc
     6601 gcctccgcga cattaaccgc gcctttcaga cggcacggga agagtgcggc tataagggcg
     6661 gttattgttt ggtttaccct atcaaggtca accaacaccg ccgcgtcatc gagtcgctta
     6721 tgtcaagcgg acaaccgcat ggtttggaag ccggttctaa agccgaactg atggcggttt
     6781 tggcacacgc cggcacccgg caaacattaa tcgtctgcaa cggctataaa gaccgtgaat
     6841 atatccgttt cgccttgatg ggcgaaaaac tggggcatca ggtttatttg gtgattgaga
     6901 agctgtccga aatacaaatg gtattggaag aggcggaaaa actcggcatc aagccccgtt
     6961 tgggtgtgcg cgccagactg gcttcccaag gttcgggaaa atggcagtct tcgggtgggg
     7021 aaaaatcaaa attcggcttg tcggcttccc aagttttgca actggtcgat attttgaaac
     7081 aaaaaaacag gctggactgc ctgcagcttt tgcatttcca tttgggctcg cagcttggga
     7141 acatccgtga tgttgccaca ggtgtacacg aatcggctcg gttttatgtt gagttgcaca
     7201 aactgggggt aaatatccgc tgttttgatg taggtggcgg gcttggcgtg gattacgaag
     7261 gaaaccgcac acaatcggat tgttccgtta attacagcct gaacgaatat gccgccacag
     7321 tcgtatgggg catcagtcag gcttgtctcg aacacgggct gccgcatccg acaatcatta
     7381 ccgagagcgg gcgcggcatt accgcacatc acgccgtttt ggttgctaat gttataggcg
     7441 ttgaacgtta caaaccgcgt cggctggatg cgccatcgcc cgaagcaccg cgtgtgttgc
     7501 acagtatgtg ggaaacttgg acggatattt ccgcctcgcg ggaaaaacgt tccttacgca
     7561 gctggataca cgaagggcag tttgatcttg ccgatgtgca taatcagtat aatgtcgggc
     7621 tgttgagttt ggcgcaacgt gcgtgggcgg agcaactgta tttaaatatc tgtcatgaag
     7681 tcggcgaatt gtttaatgaa aaacaccggt ctcaccgaac cattattgac gaattgcaag
     7741 aacgttttgc cgataagctg tatgtcaatt tctcactctt ccaatctttg cccgatgctt
     7801 ggggcataga tcaacttttc cctgtttgtc ctattaccgg tttgaatgaa ccgattgcgc
     7861 gccgcgccgt gttgttggac attacctgcg attcagacgg tacgattgac cactacatcg
     7921 acggcgacgg catcgccggt acgatgccta tgcctgatta tcccgaagaa gagccgccgt
     7981 ttttaggctt ttttatggtg ggagcatatc aggaaatact cggcaatatg cacaatcttt
     8041 tcggcgacac tgccactgct gatgttgttg taagggaaga cggacaattt accgtcatcg
     8101 attacgatga aggaaacacc gttgccgata tgctcgaata cgtttatcaa gatccgaaag
     8161 agctgatgaa acgctatcgc gaacaaatcg aacattcaga ccttcctgcc tcgcaggcta
     8221 tgtctttctt aaaagaactc gaagcggggc ttaatggtta tacctatttg gaagacgaat
     8281 agacgcatca aggtatcgga tatgtcgtct gaagcccgat tttcttactc aaacaccaat
     8341 catcacgacc gattgaaacc aattacaagg aatcattacg atgcaataca gcacactggc
     8401 aggacaaacc gacaactccc tcgtttccaa taatttcggg tttttgcgcc tgccgcttaa
     8461 ttttatgccg tatgaaagcc atgccgattg ggttattacc ggcgtgcctt atgatatggc
     8521 ggtttcaggg cgttccggcg cgcgtttcgg tcctgaagcc atccggcgcg cctccgtcaa
     8581 cctcgcttgg gagcaccgca ggtttccgtg gacatttgat gtgcgcgaac gcctgaacat
     8641 tattgattgc ggcgacttgg ttttttcttt tggcgacagc agggattttg tcgaaaaaat
     8701 ggaagcgcac gccggcaaat tactttcttt cggcaaacgc tgtttgagtt tgggcggcga
     8761 ccatttcatt accctcccgt tgttgcgcgc ccacgcccgc tatttcggca aactcgcact
     8821 gattcatttt gacgcgcaca ccgacaccta cgacaacggc agcgaatacg accacggcac
     8881 gatgttttat accgccccca aggaaggcct catcgacccg tcccgttccg tacaaatcgg
     8941 catacgcacc gaacacagta aaaaattgcc ttttactgtg ttgtccgccc ccaaagtcaa
     9001 tgaagacagt gttgaagaga ccgtccgtaa aatcaaagaa accgtcggca atatgcccgt
     9061 ttacctgact ttcgacatag actgtctcga cccgtcgttc gcccccggga ccggtacgcc
     9121 cgtatgcggc ggcttgagca gcgacagggc attaaaaatc ctacgtgggc tgacggatct
     9181 cgacatcgtc ggtatggatg ttgtagaagt tgccccctct tacgaccaat ccgacattac
     9241 cgctttggcc ggcgccacaa ttgccttgga aatgctttac cttcaaggtg cgaaaaagga
     9301 ctgaacgtcc ggcatccccc gggttttcgc cgtgccgttc aaacggcgta ttcagtctaa
     9361 tgaaaattca aatactgaaa caaaagttgc ccggagccgc atatcggaaa gacggtgaaa
     9421 tatcgtaata taacttataa aacaattagt taaatattat ttttccgatt tttcgggacg
     9481 gtctttttta cggaggtcaa tatgatgaaa ttgggtttca aaccgatacc cctcgccatt
     9541 gccgcagtat tgtgcgccct ggttttggca ctgcccgtac ccgacggggt caagcctcag
     9601 gcttggacgc tgctggctat gtttgtcggt gtgattgccg ccattatcgg caaggttatg
     9661 ccgttgggcg cgctgtcgat tattgccgtc gggttggtcg cagtaaccgg cgtaaccgcc
     9721 gacaaaccgg gcgcggcgat gagcgatgcg ttgagtgcgt tcgccaatcc gttgatttgg
     9781 ctgattgcca tcgcagttat gatttcgcgc ggtttgctca aaacagggct ggggatgcgt
     9841 atcggatatt tgtttatcgc cgtttttgga agaaaaacgc tgggcatcgg ttacagtctc
     9901 gctctttccg aactgctgct ggctcccgtt accccttcca ataccgcgcg cggcggcggc
     9961 attatacatc cgattatgca gtcgattgcc ggcagttacg gctccaatcc cgcaaaaggc
    10021 acagaaggca agatgggtaa atatttggct ttggtcaatt atcattccaa tcccatttcg
    10081 tcggctatgt ttattactgc aactgccccc aaccctttaa tcgtcaactt gattgccgaa
    10141 aatttaggca gtagtttccg tctttcttgg ggggcgtggg cgtgggcaat ggctgttccc
    10201 ggcgttatcg cctttttcgt tatgcctttg attttatatt ttttgtatcc gcctgaaatt
    10261 aaagaaacgc ccaatgctgt tcaatttgcc aaagaccgtc tgagcgagat gggtaaaatg
    10321 tcggcagacg aaatcattat ggcggtcatt ttcggtatct tgctgctgtt gtgggcagat
    10381 gttcccgccc ttattaccgg caatcacgct tttagtatca acgccaccgc caccgcattt
    10441 atcggattaa gcctgctttt gctttccggt gtattgactt gggacgatgt tttgaaagaa
    10501 aaaagcgcgt gggatacgat tatttggttt ggcgcattga ttatgatggc cgcattttta
    10561 aataaactcg gactgattaa atggttctcc ggagtgttgg cggaaagtgt cggcggtttg
    10621 ggcgttagcg gcacggctgc gggcgtaatc ctcgtgcttg cttatatgta tgcgcattat
    10681 atgtttgcca gtactactgc acatattacc gctatgttcg gcgcatttct cgctgctgcc
    10741 gtttcactga atgccccggc gatgccgacc gcgctgatga tggcggccgc atccaacatt
    10801 atgatgaccc tcactcatta tgcgaccggt acttcacctg tgattttcgg ctcgggctac
    10861 accacaatgg gagaatggtg gaaggcgggt tttgtcatga gcgtagtcaa ttttctgatt
    10921 ttttccgtta tcggcagcat ttggtggaaa gttctgggat attggtaaag ggaaaaataa
    10981 atttccaatc tgtgtttatt tgattgggcg actattatcg tgaaatatgc cgtctaaagc
    11041 cttcagacgg catatttgtg cgcttggatg ttgcagaaag cggcagggcg gcggtgtagg
    11101 aaaagccaaa caaaaaccaa accgcctgtc aacttctgat aaacataagt attaaataat
    11161 cagaaggtta ttcaattacc taaacgcaaa ttcccctgcc gtatcacatc tattgaaaat
    11221 aatacatcaa ccggctcgga agcagcctga tcaggtgatt ctacttgcgg cgatgaatcg
    11281 gcagccggtt cggtataggc agtcggcgtg ccgtcggatt gggtatttaa atgcgcttgt
    11341 gcctcgggcg tgtgtacatc aggcacttcg ggcgtgcgtg tctcggatac ttcggcagag
    11401 ttggtttcct cagtttgttc aatgacttca gcttggctgt atgaggaaga accctgtatc
    11461 cacgccagcg atttgagcgg catcttcatc ttgccgtttt tgccgcaggt cagacagacg
    11521 gccgatccgg tgcggtcttt gagtacggca tcgactttct cgggcgcgat ggttttgccc
    11581 tgtgtttttg cccattgccc gatgcttttt ttcagggagg cggtgtcaag gttgttctta
    11641 ccgtaagggt cgcggaaggc ggcaagccac aggttgcgat ccgcatcttc attgaggccc
    11701 gccatttgat agatgacgga ggcgggcgag ccggtggcga tggttttggc aaactcgagc
    11761 gcgtcgggcg atttcatgcg gacgcagccg tgactccgga tgcccgggac gctggccggt
    11821 gcattggttc cgtgtatgcc caaaccgagt ttcgggtctc ctaagcggac aaaaaccggc
    11881 cccaaagggt tgtccgggcc ggcggctatg gtttttacgc cgtcgccgcg ttctttctgt
    11941 atggattttg ggatgtgcca aacagggtta taggctttcg ccccgatttt atgttcgcct
    12001 agattggttt gtgtcatcgc ccgacctact gcaacgggat aaaccttggt cagtttgccg
    12061 tcggtgtaga ggaacaggcg ttgctgaggg atgttaatga agacatgttg accttgtgcg
    12121 acgggggaga catcgggaat gacggtgttt gcgtatgaaa aaccgcttat caatagtgcg
    12181 gcagtgcggc agattgtttt attcatatca aaatatggtg tgtgtccgat aggttttcgg
    12241 caaatcatac ctgaaaccgt accaatttgt gcgaaaatat gcgcttcggt acagtgcgga
    12301 cggattgggt aatggcaacg gaaacaaata tcgcggaaat ttccgccttg gattatgaag
    12361 gcaggggtgt ggcaaaggtc ggcggcaaaa cgattttcat taaaggggca ttacttgatt
    12421 gtttgatgct gggttgcttc aggctttaac tcaggaatat ttacatcata atgaaagttt
    12481 ttagacaaca gcttgaacaa ctcggcgcgc aaaatcaata tcgttcgatt ccggatttga
    12541 ttcatcaagg gcggtatatt acgcgggaaa accgcaaaat gctgaatatg tcgtctaatg
    12601 attatttggg gttggcatca gatgaaaact tacgccggtc ttttttgcag caatacggcg
    12661 gtaattttcc ctcttttacc agttcttcat cacgtttatt aacgggcaac tttcctattt
    12721 ataccgattt ggaagagctt gtcgcacaac gtttccaacg ggaaagcgcg ttattgttca
    12781 acagcggcta tcacgccaat ctcggtattt tgcctgcttt gacgacgacg aaaagtttga
    12841 ttttggcaga taaattcgtt cacgccagta tgattgacgg catccgtttg agccggtgtg
    12901 cgtttttccg ttatcgtcat aatgattatg aacatttgaa aaatctgctt gaaaaaaacg
    12961 tcggaaaatt tgaccgcact tttatcgtta ccgaatctgt tttcagtatg gatggcgatg
    13021 tggcggattt gaaacagctt gtccaattaa aaaaacagtt tcccaatact tatctttatg
    13081 tggatgaagc ccacgcaatc ggtgtttatg ggcaaaacgg attggggatt gccgaacggg
    13141 ataatctgat tgccgagatt gatttattgg tcggcacttt cggtaaagcc ttagcctcgg
    13201 tgggggcgta tgccgtctgc aaccaagtat tgaaagaatg tttgattaat caaatgcgcc
    13261 cattgatttt ttcaaccgca ttgccgccgt ttaatgtggc ttggacttat tttatttttg
    13321 aacgattgcc gcaattctca aaagaaagaa gtcatcttga gcagttaagc gcatttttac
    13381 ggcgggaagt ggcgcatcgg acgcaaataa tgccgagcga aacctgtatc gtcccctata
    13441 ttttaggcgg gaatgaagcc acccttgcca aagcggaata tctgcaaggt cagggttatt
    13501 attgcctgcc catcagaccg ccgacagtac caaaaaacac atccagaatc cgcctgtctt
    13561 taactgccga tatgacaacg gatgaagttc ggcagtttgc ggcgtgcctg taaggatatg
    13621 atatgaaaac aaaattttac aatcatcaag gcgaacattt aatcctgtat tttgcaggtt
    13681 ggggaatgcc gcccgatgct gtaaatcatt tgattttgcc ggaaaatcac gatttattga
    13741 tttgctatga ttatcaagat ttaaatttgg attttgattt ttccgcctat cggcacatcc
    13801 gtttggtggc gtggtcaatg ggcgtttggg cggcagagag ggcattgcaa ggaataagat
    13861 taaaatccgc aacggcagtg aatggcacag gtttgccttg cgatgataat ttcggtatcc
    13921 cttgcgccgt ttttaaaggc acattggaga atctcacaga aaatactcgt tcaaagtttg
    13981 aacgtagaat ctgtggcgat aaagcatctt ttgaagatta ccaacaattt cccgcccgtc
    14041 cgtttgacga aattcatcaa gaacttaccg cactttttgc gatgatcggg caagataggc
    14101 gtacagatct tatccgctgg acaaacgcct tgttcggatc gggcgataaa atttttattc
    14161 ctgccaatca gcaccgatat tggacaccgc gttgcaccgt tcaagaaaca gacggcgggc
    14221 attacctgtt ttcaagattt acccattggt cggcactatg gaatcactga ctgccataaa
    14281 taaaccgcgc attcggcagg ctttccaaaa agcattaagc gattatgaca ggcacgcctt
    14341 aatccaacaa aaaatgacga ttaatctaat cgcgcatttg caagattatt tgccggatat
    14401 gccattggaa aacgtgttgg aattgggctg cggctcagga atgttgagtg ccttgctgca
    14461 aaaacagatt tcagcgaatt attggttatt taatgatttg tgcgatgtac gaagccgact
    14521 ggctgaaaaa ttgcctcaat cctttgattt ttattgcggc gatgcggaaa actttccttt
    14581 tcaacgacaa tttgacttaa tcgcaagcgc atctgccgtg caatggtttc accaacccga
    14641 cgcttttatc gcccattgca aaacaggctt gaaaacaaac ggattattgg cggttgcgac
    14701 ctttggcaaa gacaatttaa aagaagtccg ccaaatcaca aatataggct taaattaccc
    14761 gactttatcc caatggcagg cttggttagc caaagatttt gagcttttat ggtgtgagga
    14821 ttttaaggta atactagact ttgatacgcc gttagatgta ctcaaacacc ttaaatatac
    14881 aggcgttacc gccacgaacc aaaaaaattg gacaagaaaa aatctcaatg gatttattgg
    14941 cgattacttg tcggcgttcg gtatgccgtc gggcaaagtg cgcctgactt atcatccgtt
    15001 attttttatc gcgcgccact ctgcggcggg caggcaataa ggcagcttat gggcaaagtt
    15061 atttttatat cgggtattga tactgatgtg ggtaaaaggt aatatggcga gccttatgca
    15121 gaaggcatat tgtcaaacgt taaattatgg tatgatttaa aatttacaag tctatttcag
    15181 taaatcgtta ataataaaag cggacaattt aataataaaa gcggataatt ggccgttgca
    15241 ggcggtcagc tatccgctcg ttacggtatc gggggtttcc tttggaaaac tcattaatta
    15301 tcggtctggc actggcggta ttgctgatgc ttttggttat gcgtgcgaag cagggtaaga
    15361 aaacaccgaa gcgcaaatcc caaggtgtcg gcaatacgca ggggcagccg cccggaagca
    15421 atgattcgga ctgggtcgat cagacttggt caatcggttt cagacggcac gcaactcgac
    15481 tggtcttgga acgaaagtgc cgagaccgca tccgccgccg tatccgcgca agaagtcgat
    15541 ccgcttacgg agtatcaggt ttataagcaa ttcggttatc agggcaaggc tgccgaatct
    15601 ttggctgcct atctggacgg cattccggat ggtgaagcga aacctgagaa ccttatccgc
    15661 gagctgatcg atatcaatct cgaagtgggg gatgtcgatg ttttggcaga caatctgcaa
    15721 aaatacggca gactgattcc tttcgaactt ttggcaaaat atatcgaaca ggcattacag
    15781 cgagattcaa accatttgcg tatccgcgtc ttggcggaag aaggtttggg atagggcact
    15841 caggagattg aaaaacgtgc ggaaggcggt tctgcgacgg cagcttccgc atcgcccccg
    15901 ccggatgtcg gcggtaaggc ttatggagcc gaagaaatca agcgcatccc gattgtgcgg
    15961 ggcaaaaaag acgtgtccgg aatcagccaa gagaaaatcg gtgcgattgc cggcctggtc
    16021 cgcgccgatc aaggtgcgaa aatccttaaa gacaaagtca gccacgaaac ggcatcgaaa
    16081 caatacgacc gtgccatcca aacttccgaa aaacctgcaa acctgattat cgatgcgttg
    16141 aaactcgatt accaacacgc ggacataggc cgttttgccg gacatttgtg gaaactttac
    16201 caaacgttgg gcaactacgg caggcaggtt aaagagcgga tgctgggtta ggggtacagc
    16261 ctgggttacc atgaagtttt cgatgatttg gaaaaggggg cgaacgatcg gtaaatcaaa
    16321 gacatcggta tgaggcacgg gtatctgccg aaaaatatac agaaattcaa agcgcaacat
    16381 cgggatttgg tgcttcaaga ttcttcgttg atcaacactg gttcgtctcc ggcagacgat
    16441 gcggttaaag aagtagagtc gttgctgatg tatggtcaga ttgaagcggc aatggatgtg
    16501 ttggagcagg cggtattgaa atatcccgac gaatcccagc tttatattac gttgatcgat
    16561 atttatgaac gtactgaaga ttgggatagg ttggggcagt ttttaagggt attgagggaa
    16621 cgtgcggaca ggcttcctga agaggtcgtt atgctgatga gccggctgct gcagcgtatg
    16681 aatcaaaata ttaaaaaaat aaaacggtac ggaaaataaa aatggaagtt caactgccga
    16741 aaattaaaac agtacgcgta atgttggcgg ggatgacggc gcagcaggaa tccgttttca
    16801 aaatggcatt caaaatgcac aataccaccc gttatgaaac agtatcccct tcagacggca
    16861 gtgccgtgcc cgatttggtt ttggcggata ccgatgccga gggcggtttt gaactttgga
    16921 aagagcttgc cgggcgttat aaggctatac ccgtcgccgt ctgttcggag aaagttcccg
    16981 attctgaagt tccctacctg cccaaaccga ttcggtttga aacattgttt cctatgctcc
    17041 gcaagttatt gcagggcgag aatgtttatg ggaaatcgtt tattgcaccc gcagaccggt
    17101 cggcgaaaaa caacgggaat gtgcagcgta cggttacgat acgccagttt aacccgaata
    17161 aaggattatt gggggcgttg cggtttgcgg aaaagaacag gcaggacatc gctatcttgc
    17221 atggaaataa gccggtcctt aattgttttc ccctcgatac aacgggtttt gctgacagaa
    17281 agtgtgtaaa aactcgaaga attgtgcaaa gacgaaaatt tgcaggtcag ttgcaagact
    17341 gttcccgata actcgcaatg gcgcgaaaag gctaaagtag gcattatgtc ctgtatgtgg
    17401 cagttttcca tttggacggc gcaggacagg ttgatttatc cgatttttcc cgatactccg
    17461 tttacgttga aatcttggcc aaacctgacc cggtaggcaa atgtgccggg gtcgatacgc
    17521 ttgtcggcat ttttgaccaa ggcatccgtc aaccttaacg tgttgtataa agtgatgcct
    17581 ttaaacctca atgatattct gaattatctt gcggcaacct atacaaccgg gtttttgtcg
    17641 gtagatttga aaacggtttc acaacaggca tactccgata tggcggataa aataaatatc
    17701 ggggccgatt ctgcttctga tagtgaaatg atgaaaaaag cggaaaaaat cacgacacca
    17761 tcccaatccc agtcgcgcgg ccttctgcaa agactgatga aaaaactgtt gggcagctaa
    17821 gaggcggaga gatgagagaa aataaaatta ttttcacagg acctgtcggc gtagggaaaa
    17881 ccactgccat tgcggctatt tcggacgaag cactcgtcca gaccgatgct tccgcatccg
    17941 acatgacttt ggataggaaa aggaatacga cagtggcgat ggactacggg ccatcagctt
    18001 ggatgaggat accaaagtcc atttatatag tacgcccggt caggaacggt tcaactttat
    18061 gtgggaaatc ttaagccaag gcagtatggg tttggtattg cttttagata atgcccgaac
    18121 caacccgttg aaagatttgg aattcttttt acattcgttt cgagggctgc tggagaaggc
    18181 atccgtcgtt gtcggtatta ccaagatgga tatacgctct cagcccggta tcgacgtgta
    18241 tcacaaatat cttgcaaaac ataatcttaa tgttccggtt tttgaaattg atgcccgtaa
    18301 ggaagatgac gtaaaacaat tggttagcgc aatgttattt tctattgatc cgggactgga
    18361 ggtttaatat ggaatcaaca ctttcactac aagcgaattt atatccctgc ctgactcctg
    18421 ccggtgcatt ttatgccgta tccagcgatg cccccagtgc cggtaaaact ttgttgcgca
    18481 gcctgttgaa agcggatgcg gacgaagtgg tcagcagtga gaagctgctc gcgggcggac
    18541 accgccgaca tcgataccgc tttgaacctg ttgtaccgtt tgcaaaaact cgaattcctc
    18601 tatggcgatg aaaacggtca ttcagacggc atcaatttgt cggacgagca attgccgttg
    18661 ctgatggaac aattgtccgg cagcggtaag gcattattgg tcgatcggaa cggtctgtat
    18721 cttgccaacg ccaatttcca tcatgagtcg gcggaagagt tggggttgtt ggcggcagaa
    18781 gtcgcacaga tggaaaagaa ataccggctg ctgattagga acaacctgta tatcaacaat
    18841 aacgcttggg gcgtttgcga tccttccggt cagagcgaat tgacattttt cccattgtat
    18901 atcggttcaa ccaaatttat tttggttatc gccggcattc ccgatttgag caaagaggca
    18961 tttgttactt tggtaaggat tttataccgc cgttacagca accgcgtgta aaacttggga
    19021 caggagggat tatgcagcaa ttattgattt caatccttga agatttaaat aatacatcta
    19081 cggatattat cgcgtctgcc gttatctcaa ccgacggatt gccgatggcg acaatgcttc
    19141 cttcacattt gaattcggac agggtagggg cgatttctgc cactttgctt gctttgggga
    19201 gtcgctcggt gcaggaactc gcctgcgggg aattggaaca agtgatgatt aaaggaaaat
    19261 caggctatat ccttttaagt caggcgggta aagatgccgt gttggtgctg gtggcaaaag
    19321 aaaccggcag acttggttta atcctattgg atgccaaacg tgcggcaagg catattgcgg
    19381 aagccatata acatataaag attgcgggct tgcagataag tgcaatcgat tgtcaattta
    19441 tattgacacg ttcggtattt ctgttttatt attcgcgttt actccccgat agctcagtcg
    19501 gtagagcgac ggactgttaa tccgcaggtc cctggttcga gcccaggtcg gggagccaaa
    19561 ttccaaaacc ctctaagtat tttcttagag ggttttgttt taccggcggt cagaaacgca
    19621 tttttgagat gattgttttg agatggaata aaatctttgc aaaattcctt tcgtgatggt
    19681 tatgaaaaat aaatttctaa agatttaatt ggttattttt ataattttga aatttatcgg
    19741 ttttgtaaag gtctcgggat aagcaatttc ggtcttcggc gcgtctttta cgccaaaccc
    19801 ataagaattt tgagggtaat cttcatcgtt ttgcctgaat tgccgacgca aagcgattta
    19861 tcggcggttg tgtttgagcg tttgattgca gcttgcccaa tctcaaatat ttctaaatac
    19921 caattgaaat tcaatatttt ataaaaacac tgatttggaa cgaacagatt tataaaaatt
    19981 ccgtgtatcc ttgagtaagg gagttgattg aaaagacttg tttgattgat gatgaagaat
    20041 aagttaatta atgtcttatt tggtattcgt tctattcata ttttttccca gttccttcac
    20101 taaaaaatcc aaaaatacgc gtaagcggag gttgacggct ttatcgctgt aataaacagc
    20161 attaaagggg tgtgttttat tggaggtttg ttcggcgaat aggggaatta actttccttc
    20221 agtgatgtcg ttgtcaacca aaaaatctga taagcaagca ataccgcaac ttgaaaggca
    20281 caacgagcgt aagatttcac cgctgctggc ggtaaagtgc ggtgaaattt tatagggatt
    20341 tccctgcgca tctaaaaccg cccatgtatt tagagaaccg ggttctgtga agcctaaaca
    20401 ttgatggttg gcaagatctt ctgcagattg tggcgtgccg tgttttgcta aatattcagg
    20461 actggcgact acgcggaagt ggctgtcaaa cagatggcgt gcacgcagcc cggaatcgtc
    20521 caattctccg gcccgtaagg caatatcgac tttgcgttca atcagattga tatagccttc
    20581 ggaagaaacg agcgaaagtc ggatatgcgg atagcgttcg ttgaattttg ctgccagcgg
    20641 cgccagcaga tgcagcacca tcggcatcgc ggaatccacg cgcaacacgc cttgcggtac
    20701 ttcgtgcact gccagcattt cggtttccgc cgctgccatt tcttgcagga ttctctgcgc
    20761 gcggcggaaa tattgcgcgc cttcttccgt cagattgagt tgccgcgtgg tgcggttgag
    20821 caggttcacg cccaactttt cctccagccg tttgacgatg cggcttacgg cagaatttgc
    20881 catctccaac tgctccgccg cacggctgaa gctgccgctt tccaccactt gaacaaatac
    20941 ggtcagttct tctgaattgg ttttcatcgt gtttcctttt cggttggaac cccacccttt
    21001 agggcggcag gatcagactt tatttgggaa gggcgtaacc cattcccaat caggacggca
    21061 catagggaga cgatttatgt gtcatcccgt gtgttgaaac atctgattat tccatttgac
    21121 gcaaaagtgt tttcttattt ttgcactttt aaatcataaa gtaaaacggt acaatacatt
    21181 catcaattca caaacgaggt aacaaatgaa tattttatta ttagacggcg gcaaagcgtt
    21241 cggacattct cacggcgggt taaaccacac gcttcacaaa aaagcgaaag aagttttgac
    21301 cgcgctcgga cacaatgttt aagaaaccgt gattgatgcc ggctatgatg ttgaggcgga
    21361 aatcgaaaaa ttcgtttgga tggatgctgt gatttggcag atgccgggct ggtggatgca
    21421 cgagccttgg acagtgaaaa aatatatgga cgaagtcttt accggcggac acggcaaact
    21481 ctaccaaagc gacggcagac accgcgtcaa tccgactgag ggctacggca caggcggctt
    21541 gttgcaaggc aaaaaacata tgctttcact gacttggaat gcgccgattg aagcctttac
    21601 ccgcgaaggc gatttctttg aaggcaaagg cgttgatgtt ttgtatatgc acttccacaa
    21661 agccaacgag ttcatcggct tgagccgcct gccgacattc ttatgtaacg atgtggttaa
    21721 aaatccgcaa gtggaaaaat acttggcaga ttatcaggca cacttggaaa aagtgttcgg
    21781 ctaattaaaa atccatcttc aacacggaga tggattttgt ttgtttcgtt gattttgtgt
    21841 cagtttcaga tgtgggtgct tatccggacg ggtcgtctga aaatgtttgc cccaatgcaa
    21901 aaaaatcact gcaaaccttc acaaacgggg ctggtagtga ttttttcaaa tcaaacagat
    21961 tgaaaacctg cgccgaattg ttcagacggc attatttttt caattccgcc aaaatcccgt
    22021 caacggtttt cttcgcgtcg ccgaaacaca tcacgctgtt ttcgttgaag aacagcgggt
    22081 tttgtacgcc tgcgtagccg gtgttcatcg agcgtttgaa tacgacgact tcttttgcct
    22141 tccatacttc taaaaccggc atacctgcaa tcgggctgtt cggatcggtt tgggcggcag
    22201 ggttgacggt atcgttcgca ccaatcacca agaccacatc ggtttcgggg aagtcgtcgt
    22261 tgatttcgtc catttccaaa acaatgtcgt aggggacttt ggcttcggcg agcagtacat
    22321 tcatatgacc gggcaggcgg ccggcgacgg ggtggatgcc gaagcgtact tcgatgccgt
    22381 ttttacgcaa aagctcggtg atttcggcaa cggggtattg tgcctgcgcc actgccatgc
    22441 cgtagcccgg ggtgataatg acgctgcttg cgcctttcag catttcggca acatcggcag
    22501 ctttgacttc tcggtattcc tctatctctt ggctgccgga agacgatatg ccgctgtcgc
    22561 tgccgaaacc gccggcaatc acggaaacaa acgaccgatt catggctttg cacataatgt
    22621 aggacagaat tgcgccgctt gagccgacca gcgcgccggt aacgatgagc aggtcgttgg
    22681 agagcataaa gcctgccgct gcggccgccc agccggagta ggagttgagc atagatacga
    22741 ccacgggcat atccgcgccg ccgatggagg caaccaagtg ccagccgaat gcgagggcga
    22801 tcagggtcat aatcagaagg atgaagccgc tgccgtcaat gccgacaaat acgagcagca
    22861 acacaaacga tacggcgagc gccagtgcgt tgagcttgtg tttggcgggc agttgcagcg
    22921 ggctgctgct gattttgccg ttgagtttgc cgaatgcggc cagtgagccg gtaaaggtta
    22981 ccgcgccgat gaaaatgccc aaatagactt cgaccagatg gatggtgtgc atatcgtgcg
    23041 aaacgttgcc cggctcgatg tagctgttga agccgaccaa aacggctgcc aagccgacga
    23101 agctgtgcag aagtgcaatc agttcgggca tttcggtcat ttctactttt ttggctttgt
    23161 ggatgccgat tgccgcgccg atgagcatgg cgatgataat ccagcccagt ccgtgggtat
    23221 tgtcggaaaa gaccgttacg aagagggcga ccgccatacc ggcgataccg gaatagcagc
    23281 cccgtttggc ggtttcctgt ttggacagcc ccgccagtga gaagatgaat aaaattgcgg
    23341 caacgatata cgccgctgtt acgagtcctg aagacatgga aattctccga ttttcgatga
    23401 tttgttttca atgccgtctg aaaaattgac gttcgtgttt tcagacggca tctgtttcaa
    23461 gcagccgcga caaacagtcc gacggtgcag gcaaatccgc ccgcccacat cagcgagcgc
    23521 aatgctgctt tgtctgcgat gtagcaccag ataaaggcga ggcggaacag gatgaacaat
    23581 ccggcaagcg tgttgacggt tgcttgtccg gcattgccgg ttgcgtgtgc cgtcaaaacg
    23641 gcggcggcaa acggtgcaaa ggcttcaaaa ccgttttgct gcgcggcgtg ggcacgggcg
    23701 gctgcgcctt gcgtatgtgc cagaaaaccg cgaggattgt ggttgtcttt aaaccggaat
    23761 ccgcctgctt ttttggcata cgccgcacaa aaaagcggca ataggcaggc aatcagaatg
    23821 caccaatagg cgaaagtcat ggcttaccct ttcttaaaca tattcagcat acgccgtgta
    23881 accgcaaagc cgccgaagat attgatgccg gcaatcagga tggcaacaaa cgacagcagc
    23941 gaaacgaagc cgttgccctg accgatttgc agcagcgcgc cgacgacgat gatgccggag
    24001 atggcgttgg ttaccgacat cagcggtgtg tgcagcgagt ggctgacgtt ccaaacgaca
    24061 tggtagccga tgacgcaggc gaggacgaag acgataaagt ggttcaagaa tgctgcgggt
    24121 gcgaccgcgc cgacccacag caccaatacg gcggcgatgg cggcgggcgc gagttttttc
    24181 cacaggggaa caggtttcgg ctcgggcttg gcggcaggcg cggctttttc agacggcgtt
    24241 tgctgcggcc gggcggaaac ctgaatcggc ggaggcggga aggtgatttc gccgtcgcgg
    24301 gtaacggtca tattgcggat aatcacgtct tcgaagtcca gcgtgatttc gccgtctttg
    24361 ttcgggctta acagcttggt caggttcacc aagttggtgg cgtaaagctg ggaagactgt
    24421 ccggcaaggc ggtttgccat gtcggtgtag ccgatgattt tcacgccgtt gccggttacg
    24481 gacaattcgc ccggtcgggt gagttcgcag ttgccgcccg tcgccgccaa atcgacgatg
    24541 acggatccgg atttcatgct ttccaccatt tctttggtaa tcagcttggg agcgggtttg
    24601 cccggaatgg cggcggtggt gatgatgatg tccacttctt tcgcctgttc ggcaaagagc
    24661 ttcatttcgg cggcgataaa ttcgtcgctc atcactttgg cgtagccgtc tccgccgccg
    24721 cccgattctt gcaggaagtc gagtttcagg aacttaccgc ccatcgattc gatttgttcc
    24781 gccacttcca agcgggtatc gaacgcgcgc accactgcgc cgagcgaatt tgccgtaccg
    24841 attgccgcca aacccgccac acctgcacca atcaccaaaa cctgcgcagg cggcactttg
    24901 ccggcggcag tgatttgacc ggtgaagaaa cggccgaagg cgttggcggc ttcaatcacg
    24961 gcgcggtagc cgctgatgtt tgccattgaa gacaaagcgt ccaaggcctg agcgcgggaa
    25021 atgcggggaa ccatgtccat cgccagcgcg ttgactttct tggcgcgcaa ggcctcgacc
    25081 aaagcctcgt tttggcgcgg ccacaggaag ctgacgatgg tttgaccttc tttgagcagc
    25141 ggcagctcgc cttcggacgg cgcgttgacc ttataaatta aagggcaggc ccaaaccgcc
    25201 gctttgtcgg caacggttgc gcctgctgtt tggtaagcgg catcgtccaa acttgccgcc
    25261 aaacctgcac cgctttcgac aacggtttca aagcctagtt tgcccagcag ggcaacggtg
    25321 gcgggcgtgc aggcgacgcg ggtttcgccg gataatgact cgcgtgggat accgattttc
    25381 atctctgaat cctttttcgg gttgttcata tgtatcgtgg gttaaattta aatcggtgca
    25441 gggcggagca acgccgtacc ggtttaaaac cgattcactt caaatgttaa tatattttag
    25501 ataatcccct tataacgaat tttcatcagg ctggcaatag ttgcgcattt tcccgtgttg
    25561 tccgacacat actgccgttt gcgtttgaag tcgttttccg attgcaggca cgcccgccgt
    25621 aacattcttt gccgtttcac tatataatcc gcattttttg agccgccgtt atgccgcaag
    25681 ccctcgtcct ccaatttccc tccgccgaag ccctgccttc cgacttcccc tcacgcctgc
    25741 ccgaacctga ttatgctgat gaaaagcgta tgcgttttat tgttgaagaa ggattttctt
    25801 taagcgaaaa agacgcggcg ttactggaca gccgtcaaat cgaccacgcc gttttgccca
    25861 atatggcgtt tggcgaactc gggctgattg tcagcgatat ggattcgacg ctgattacca
    25921 tcgaatgtat cgatgaaatt gcggcaggtg tgggtttgaa aggcaaagta gcggaaatta
    25981 ccgagcgcgc catgcgcgga gagttggatt tcgaacagtc tttacgcagc cgcgttgcgt
    26041 tgttggcggg attggacgaa cagattttgg cggacattta tgaaaacgtt ttgaagctct
    26101 cgcccggtgc ggaatttttg ttggacgaat gcaaaaggca caatgtgaaa ttcctgctgg
    26161 tgtccggcgg gttcacgttt tttaccgaaa ggctgcaaca acggctcggc ttcgaatacc
    26221 aacacgccaa tattttggaa attgaaaacg gcaggttgac cggccgtctg aaaggcagaa
    26281 tcatcgacgc gcaggcaaag gcggatttat tgcgcgaata ccgcagccgc ctcggattgc
    26341 agccgcatca ggtgttggcg atgggcgacg gtgcgaacga tattccgata ctcaaagaag
    26401 cgggcatagg cgtggcttac cgcgccaaac cgaaagcgcg ggccgccgcc gatgcctgta
    26461 tcaacttcgg cggtttggag cgtgtacgcg gcctgttcgg ataggcggat aggaaacgga
    26521 tgccgtctga aaggttttca gacggcattt gaacggcagg aacgacagtg ggacgcagaa
    26581 aactttggtt tgccctggcg gcagcaggcg ttatcggcgg tttggtcggc attgtgctga
    26641 cggaactgat gcacttcata cggcatacgg catacggtta tgacgcggac ggtgcgtaca
    26701 tctcgttccg cgaaggtgtg gcacaggctt tcggtatgcg gcgcgttgcc atgctgacgc
    26761 tgtgcggcgc gatcgcaggc ggcggctggt ggctgctcaa acgtttcggc aagccgcaaa
    26821 tcgaaatcaa agccgccttg aagcagccgt tgcaggggct gccgtttctg acgacggttt
    26881 tccatgttct gctgcaaatc ataacggtcg gactcggttc gccgctcgga cgcgaagtcg
    26941 ccccgcgtga aatgactgcc gcgtttgctt ttgccggcgg caggcggttg ggtttggacg
    27001 aaggcgaaat gcgcctcttg attgcctgtg cgtccggagc gggtttggcg gcggtgtata
    27061 acgtgccgct cgcctccacg ctgtttatcc tcgaagcgat gttgggcgtg tggacgcagc
    27121 aggcggttgc cgccgcattg ctgacttccg tcatcgccac cgccgtcgcg cgcatcggct
    27181 taggcgacgt gcagcagtat catcccgcca accttgccgt aaatacttca ttgctttggt
    27241 tttccgccgt catcggcccg atacttggcg caaccgccgt ctttttccgg cgcaccgccc
    27301 aaaaattccc cttcatcaag cgcgacaata tcaaaatcat tcccttggcc gtctgtatgt
    27361 ttgtactcat cggtgtgatt gccgtttggt ttcccgaaat tttgggcaac ggcaaagcag
    27421 gcaaccaact gacttttggc ggtttgaccg gctggcaata cagccttgag ttgaccgctg
    27481 tcaaatgggc ggtcgttttg atggcgctgg cggcaggcgc atacggcgga ctgattacgc
    27541 cgtctatggt gctcggcagc accatttctt ttgcagccgc cgccgcgtgg aacagcgtct
    27601 tccccgaaat gccgtctgaa agcgcggcag tcgtcggagc ggcggtgttt ctcggcgtat
    27661 cgctcaaaat gccgctgacc gccattgtgt tcgtattgga actgacctac gcgccgctgt
    27721 ccctgctgct gcctttgtgc atcggcatgg cgggggcatt gggaacatcg ggaaaaatgg
    27781 gcttcaaatg aatgccgaca aatgccgtct gaaaggtttc cagacggcat ttctttgcaa
    27841 attgtcaaca atatctgcaa aaacctactg ccaaaaaact gaaaatagcg gataataccg
    27901 ccccgaaaaa gatccccttt caccatccaa ccgtcagaaa aggaagcccc aatgtccgtc
    27961 atcaaacgcg ccctgatcag cctgtgcgac aaggcaggcg cggtcgaatt tgcccaaacc
    28021 ctgcacaagc tcggtgtcga aatcctctcc accggcggca cggcgaagct gcttgccggt
    28081 gcgggcgttc ccgtgattga agtcgccgac tacaccggtt ttcccgaaat gctcgacggc
    28141 cgcgtgaaaa ccctgcatcc gaaaatccac ggcggtattt tgggccggcg cgatttggac
    28201 gaacacgtcg ccaagatgga agagcacggc atcggcaaca tcgaccttgt gtgtgtcaat
    28261 ctttacccct tcgccgccac catcgccaag cccggctgca cactggaaga cgcaattgaa
    28321 aacatcgaca tcggcggccc ggccatggtg cgttccgccg cgaaaaactg gaaacacgtc
    28381 gccatcgtta ccgataccgc cgacttcccc gccattgccg ccgaactcga agccggcaac
    28441 ggcgcgttga gcgataaaac ccgcttcaac ctctcacgca aagcgttcag ccacaccgcc
    28501 gaatacgacg gcatgatttc caactattta acctcgcttt cagacggtgt tttgagcgga
    28561 gagcctgaaa tcggcgaatt tccgagccgg ttcaaccaaa gctggattaa agtgcaagat
    28621 atgcgctacg gcgaaaaccc tcaccaacgc gccgcgttct accgcgatat tcatcctgcg
    28681 gcaggcagcc tttccgccta caatcaactg caaggcaaag aattgtctta caacaacatc
    28741 gccgatgccg atgccgcttg ggaagccgtc aaatccttcg acgcgcccgc ctgcgtgatc
    28801 gtgaaacacg ccaatccgtg cggcgtagcc gttgcagccg ataccttggc cgcctacaaa
    28861 ctcgcctacg ccaccgacac caccagcgcg ttcggcggca tcatcgcctt caaccgcgaa
    28921 gtcgacggcg aaaccgtcaa acagattacc gacaaccaat ttatggaagt cctcatggcg
    28981 ccgaagttta ccgccgaagc ccttgaaatc gccgccgcca agaaaaacgt gcgcgtattg
    29041 gaagtgccgc tcaaagcagg tgccaaccgc tttgaactca aacgcgtcgg cggcggactg
    29101 ttggtacaaa cgcccgacat caaccgcatc aaccgcgccg atttgaaagt cgtctccaaa
    29161 cgccaaccga ccgagcagga atggaacgat ttgctgtttg tctggaacgt cgcaaaatac
    29221 gtcaaatcca acgccatcgt cttcggcaaa ggcggccaaa cctacggtat cggcgcaggc
    29281 caaatgagcc gcgtggacag cacccgcatc gccgcccgca aagcgcaaga tgccggtctc
    29341 gacctcaacg gcgcatgtgc cgcttccgat gccttcttcc cattccgcga cggcgtggac
    29401 gtgattgccg aacagggcat caaagccatc atccatccgg caggctcgat gcgcgatcag
    29461 gaagttttcg acgcggcgga cgaacacggc atcgccatgg cggtaaccgg catccgccac
    29521 ttccgccatt aaggcagacg aacaaggcaa tgccgtctga agggttttca gacggcattt
    29581 ttgcgctatt taccgtaatc ctgaaatccc gtcattccca cgcaggcagg aatctagatg
    29641 agaatccatc ctgtcagaaa tacccgaatc gtcaggaata cccgtgtcat cattcccgat
    29701 acaccgtaat cctgaaaccc gtcattcccg cgcagacaga aatccggatt tgtccgcgcg
    29761 gaaacttatg cgccgccatt cccgcgaagg cgggaatccg gaccgttcgg tttcggttat
    29821 ttccgataaa ttcctgctgc ttttcattcc tagattccca ctttcgcggg aatgacgaag
    29881 gagtgggaat ccggtttttg agttccggcc atttccgaca aattgctttg gcattggata
    29941 tttcctattt taatccacta tagtggatta actttaaacc agtacggcgt tgcctcgcct
    30001 tagctcaaag agaacgattc cctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
    30061 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccgc catatccgat
    30121 cgccgcaccg gaattttaaa aaataaatac attctttaac aaaaaaaatc atatcccggc
    30181 aaaacaaatc tgataaaatg cttggcgttt attaacaatc tttttaataa tttaatctta
    30241 gat
//
LOCUS       DAXDZG010000019        27483 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXDZG010000019 DAXDZG010000000
VERSION     DAXDZG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27483
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.019"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(66..3110)
                     /locus_tag="ACFB78_000742"
     CDS             complement(66..3110)
                     /locus_tag="ACFB78_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC1268784.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKTAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3275..3955
                     /locus_tag="ACFB78_000743"
     CDS             3275..3955
                     /locus_tag="ACFB78_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268785.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            3984..4343
                     /locus_tag="ACFB78_000744"
     CDS             3984..4343
                     /locus_tag="ACFB78_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC1268786.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            4421..4527
                     /locus_tag="ACFB78_000745"
                     /pseudo
     CDS             4421..4527
                     /locus_tag="ACFB78_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4948..5484)
                     /locus_tag="ACFB78_000746"
     CDS             complement(4948..5484)
                     /locus_tag="ACFB78_000746"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC1268787.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5518..5874)
                     /gene="folB"
                     /locus_tag="ACFB78_000747"
     CDS             complement(5518..5874)
                     /gene="folB"
                     /locus_tag="ACFB78_000747"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC1268788.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5930..6532
                     /gene="plsY"
                     /locus_tag="ACFB78_000748"
     CDS             5930..6532
                     /gene="plsY"
                     /locus_tag="ACFB78_000748"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC1268789.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHPSWIFATLAIAILVLLRHKSNILNLIKGKEGKIGEKR"
     gene            6624..7469
                     /locus_tag="ACFB78_000749"
     CDS             6624..7469
                     /locus_tag="ACFB78_000749"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC1268790.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            complement(7577..7909)
                     /locus_tag="ACFB78_000750"
     CDS             complement(7577..7909)
                     /locus_tag="ACFB78_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC1268791.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(7959..8207)
                     /locus_tag="ACFB78_000751"
     CDS             complement(7959..8207)
                     /locus_tag="ACFB78_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268792.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(8264..8425)
                     /locus_tag="ACFB78_000752"
     CDS             complement(8264..8425)
                     /locus_tag="ACFB78_000752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268793.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVEKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            8450..9424
                     /locus_tag="ACFB78_000753"
     CDS             8450..9424
                     /locus_tag="ACFB78_000753"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC1268794.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LAFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(9583..9801)
                     /locus_tag="ACFB78_000754"
     CDS             complement(9583..9801)
                     /locus_tag="ACFB78_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC1268795.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            9995..10258
                     /locus_tag="ACFB78_000755"
     CDS             9995..10258
                     /locus_tag="ACFB78_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC1268796.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIDRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            10432..12246
                     /locus_tag="ACFB78_000756"
                     /pseudo
     CDS             10432..12246
                     /locus_tag="ACFB78_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
     gene            12310..12474
                     /locus_tag="ACFB78_000757"
     CDS             12310..12474
                     /locus_tag="ACFB78_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268797.1"
                     /translation="MSNQTKIQAIKQASEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            12419..12661
                     /locus_tag="ACFB78_000758"
     CDS             12419..12661
                     /locus_tag="ACFB78_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268798.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            12728..13978
                     /gene="glyA"
                     /locus_tag="ACFB78_000759"
     CDS             12728..13978
                     /gene="glyA"
                     /locus_tag="ACFB78_000759"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC1268799.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVCGQVTALCDKYPVYGN"
     gene            complement(14051..14440)
                     /locus_tag="ACFB78_000760"
     CDS             complement(14051..14440)
                     /locus_tag="ACFB78_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1268800.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            15380..16171
                     /locus_tag="ACFB78_000761"
     CDS             15380..16171
                     /locus_tag="ACFB78_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC1268801.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPISEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGVSADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            16322..16966
                     /locus_tag="ACFB78_000762"
     CDS             16322..16966
                     /locus_tag="ACFB78_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC1268802.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            16985..18895
                     /locus_tag="ACFB78_000763"
     CDS             16985..18895
                     /locus_tag="ACFB78_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1268803.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENKKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            18917..19283
                     /locus_tag="ACFB78_000764"
                     /pseudo
     CDS             18917..19283
                     /locus_tag="ACFB78_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19395..20870
                     /gene="trpE"
                     /locus_tag="ACFB78_000765"
     CDS             19395..20870
                     /gene="trpE"
                     /locus_tag="ACFB78_000765"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC1268804.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            20954..21892
                     /locus_tag="ACFB78_000766"
     CDS             20954..21892
                     /locus_tag="ACFB78_000766"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1268805.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            21893..22753
                     /locus_tag="ACFB78_000767"
     CDS             21893..22753
                     /locus_tag="ACFB78_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC1268806.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            22770..23906
                     /locus_tag="ACFB78_000768"
     CDS             22770..23906
                     /locus_tag="ACFB78_000768"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC1268807.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            23953..24435
                     /locus_tag="ACFB78_000769"
     CDS             23953..24435
                     /locus_tag="ACFB78_000769"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1268808.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            24541..25589
                     /locus_tag="ACFB78_000770"
                     /pseudo
     CDS             24541..25589
                     /locus_tag="ACFB78_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            26169..26774
                     /locus_tag="ACFB78_000771"
     CDS             26169..26774
                     /locus_tag="ACFB78_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC1268809.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            26837..27199
                     /gene="ssrA"
                     /locus_tag="ACFB78_000772"
     tmRNA           26837..27199
                     /gene="ssrA"
                     /locus_tag="ACFB78_000772"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         7235 a         7559 c         6222 g         6467 t
ORIGIN      
        1 tgttaatcca ctataaaaat tccgtctgaa acagtcttca gacggcattt ttatttgcaa
       61 aaaaatcaag cattgtccaa ggggacgaga atcgtacggt tgccgttgtg ttccggtgcg
      121 gacacaatgc cttccgcttc catttggtca atcagacgcg cggcgcggtt gtagccgata
      181 cgcaaggcgc gctgtacgcc cgaaatgctg gctttgcgcg ttttcaggac aacggatacg
      241 gcctcgtcgt acatcggatc ggtttcgccg tcgccgctgc gcccgatgcc gggcagctct
      301 tcgctgccgc cgccgctcaa aatatcgtca acatagtccg gctcgccaaa ctgcttcaga
      361 tattcgacca cgcggtgcac ctcttcatcc gaggcaaacg cgccgtgaac gcgctgcgga
      421 taggcagtac ccggcggcag gaacagcata tcgccctgac cgagcaggtt ttccgcgccc
      481 atttggtcga gaatcgtgcg gctgtcgatt ttgctggaca cttggaacgc gatacgcgtc
      541 gggatgttcg ccttaatcag acccgtgatg acatcgacgc tggggcgttg tgtggcaagg
      601 ataaggtgga tgcctgccgc gcgggctttt tgggcgaggc gcgcaatcag ttcttcgatt
      661 ttcttgcctg ccgtcatcat caaatcggca aactcatcga ccacgaccac gataaacggc
      721 agtttttcca aaggttcggg atcgtcgggc gtgaggctga acggattgcc gattttttct
      781 ccccttgctg cggcttcggc ggttttttgg ttgaagcccg caagattgcg cacgcccata
      841 aagctcatca ggcggtagcg tttttccatt tcgttaacac accagttcag cgcgtttgcc
      901 gccagcttca tatcggtaac gacaggggcg agcaggtgcg tgatgccttc gtaaatgctc
      961 aattccagca ttttcggatc gatcataatc atacgcacgt cttccggcgc ggctttgaaa
     1021 agcatagaca gaatcatcgc gttgacaccc accgatttgc ccgaaccggt cgtgccggca
     1081 accagcaaat gcggtgcttt gcccaagtcg gttacgacgg gctgtccggt aatgtcctga
     1141 ccgagcgcga gcgtcagctt ggatttggat tcggcaaact cgggcgaatt gaaaatttcg
     1201 ctcaggcgta tcatttggcg tttcgggttc ggaagttcca aacccatgca ggttttgccg
     1261 gggatggttt cgacaacgcg gatggaagcc acgccgagcg aacgcgccaa gtctttttcc
     1321 aaattcagaa cggaattgcc gcgcacgccg acatcgggtt cgatttcata acgcgtaatc
     1381 acggggccgg aataagaatc gacaaccttg accttgactt tgaactccgc caatttttct
     1441 tcgatggtga tgctgttttc caacagttct tcttcggttt gcgtcgcctc gggattgaac
     1501 agcggaggca gaagcaggtc ggttgtcggc aggtgttcgg ataccgcacc ggtctcttcc
     1561 gattggaacg ccccttcatc cgcttccgta tccgataccc ggcaggacgg gcgttcagac
     1621 ggcacatctt caaacggaca aaccgcctga ctgtcatgcc cgaacgcttc ggtttccgac
     1681 agatattgcc cgcttgaccg ctctgccgca ccctcactgc cgtcatctgc aatagcggcg
     1741 gtttcctcct gccaacctcc attcaaaaca tcagcggcaa gatggtcggt ttcggcaatg
     1801 cggctgcgtt gcgcctgctc gaatcctgcc ggcggctcat aggtacggtt gtagatttcc
     1861 gatacgggag gcggcggcag aatatcgatt tcgggtacgg ctacctccgg cacttcgggt
     1921 ggttcgacca cccaagcatc gggcgcggca ggctcttcaa tatggatatc ggcagcttca
     1981 tcataaaccg gaccgccgta tcccacagac gaaacggttt ccgtgaaaac attttcggtt
     2041 tcagacggca tttcgacatc ccgtttcccg acaaccgtcc gactttccgg aatcaggcgc
     2101 gaaatccgcg cctccgcaga aaccgttgcc ttgttcggtt ctttcaaatt gacggaaacg
     2161 cggcggcggg catcgcggac ggctgtgccg tctgaaacgg actgcccttg ggaaacatcc
     2221 ggggattttg cctccgcctg ccctttctcc tgccctgcat gacggcggaa cggcgcattc
     2281 cgtgcatttt cggctttgac tgcagaaaag gcagacggat gctgcttctc aaaatacggc
     2341 tcaaatccgt aatccgcaga ctcggaaaac gcttctttgt ccgcatcaaa catacgggaa
     2401 tacgtgcgtt gaagcacagg gtcgtccaaa ccgataatct gcagcccttc catcggggta
     2461 tcggacacgc gtactttggg gacggctgtt ccgtttttct caaatgcatc gatatagcgt
     2521 tttttcgttt cccttaaagc cgcgctgctt aaagcacgcg ttgcttcttc caaagtgatt
     2581 tccttaaaaa ccggacggac gggcgaagtt ttgctttcag actgcatcaa cccttcagca
     2641 atactccggt cgaatgggat gcggcggttg tcgattacgg cagttgcaat gtcttccgta
     2701 tcggcagcct cttcctccgc agcttctgct gcttccgttt ccgcttcttc cgtcccgttt
     2761 ccactgtctg aatacccgtc ggatgcggtc ggaacatccg cacggtttgc agaagcaggc
     2821 ggaacgggcc tgtccggttc ggattcgtgc gccagatagt ttcggcagaa acggacgaca
     2881 ccgacaaaca gccaatacac tgccgttttg acggcatgga aaagcatcag ggaaaactcg
     2941 ggaaaatcgg gcataccgtc tttgatttca ggcagctccg cctgtttttc ccctttcttt
     3001 tcctgccacg cagaaacctc gcgcatccac tcgcgttcgg attgtgcgcg gacaaaaaac
     3061 aggccggcaa gcgcaagcaa caaaataacg atcaaaacta tccaaaacat atgccgtttc
     3121 ccgcaagaca aacgattaac cgagtttatt ttaacgtgtt tccagcccaa aaaaaacaac
     3181 cggccggcaa ttctgccgaa accgattcgg ctttcggttt gcccccgccg acagtataat
     3241 agccgcccct tcccttaatc tttgcggcat taccatgatt ttccaaacag tctggttttc
     3301 agatgtggta ctgtcggttt cgtggattgt ccttatcctg atactggcag cttccgcgcc
     3361 gtcggcattc cgttcgcttg cccgctaccg ttccgccctt cccctgtgta ccgtaatttt
     3421 ttccgcagca tggtgtctga acgcctctgt cggcggcgga cagcttgccc agatgaacta
     3481 ccacctgctt gccgtcaatc tggttacact gatggtggat acctccgccg ccctttggct
     3541 tgccgcgctg ctgatgctgc cctactgcct gctgtttgcc ggttctgccg gcgcatatcc
     3601 gccgaacgct ttggtgctga tactgcccgc gcttgtcgtc aaccgcctgt cgcgtatgct
     3661 ggtcaaccgt ctgccgccca atattttcat ctttattttc gtcaacggct ttctcgcttc
     3721 cgccgccggc attctgctga ccgggctggt gctgatcggt attttggatg ccgcaaacgc
     3781 ttttccgtcc gaaatattgt ggacggccgc cctgcccgtc tttattctgc tggcgtgggc
     3841 ggaagccttc ctcagcggca tttcaactgc cattttcgtg gcactgaagc cccattggat
     3901 caacactttc gacgacaacc gctatctgaa gtccgagcgc ggcatatggc tgtagccttc
     3961 agacggcata ggcagacaaa accatgcttt ccaacatcct ccccctttcc atcggtgcaa
     4021 tattcggcac aacggcgcgt tggctgctca acctcgccgt ccccgcatcg ttgtctcccg
     4081 ccacaggcaa cctgtttgcc aactggacgg gcgcgctgct catcggcatc tttgccgaaa
     4141 ccgtcagcca tccgcaatgg aagctgctgc tgattaccgg ttttttcggc agcctgacca
     4201 cgctttccgg attttcactg gaaaccgtaa ccctgctcca atcgaaccgt cccgcttcgg
     4261 cacttgccaa tattttccta cacacggcag gttcgctgct gctgacttgg ctcgggctga
     4321 agatagggac tgcagtcaaa taaccgcaaa aaattgcttc ctttggtttt gacataatct
     4381 ataaattggc ttcgcttgtt taaatactgc ctgtgccaat tatagtggat taaatttaaa
     4441 tcaggacaag gcggcgagcc gcagacagta cagatagtac ggcaaggcga ggcaacgccg
     4501 taccggttta aatttaatcc actataccgt tctcaaattc aaattgggct tttaggtcta
     4561 taacaaagaa aaagccttgg gaggataaac ctaccaaggc tttttcttaa aaaacgctct
     4621 atcactgtgc ctaaaaatgc aaaaaagtaa ccgtcataat ccttttgcca aaatcaatat
     4681 catcatccga tgaatgaacg gacggtatat aagaaagcgt taataaactc ataaaccagt
     4741 aagaaaaatc agtaacgaag atattcatta ttacccattc ttaaaaatta tcacgttcgt
     4801 ttgggtaatt atcataccct tttccctctt attactaaag cacgaattac tttaaagtac
     4861 gcacccagag acaagccgaa aaggcatatc cgatccgcca aacaaaatgc ccgccgactt
     4921 actccggcgg gcggttccaa catcctgtca atccttcatc agccagtatt gcaaaccgat
     4981 taatgtttta ccgtctttaa tttcatcgtt tgccaatgcc tgacggactt catctttcga
     5041 catcagcacg gtttccgtta tttcatcttc atcattagaa agcgtactgc ccaagcgcac
     5101 gccttccgcc tcgaacagat acattttttc attgcaaaaa ccgaccgccg tataaaaact
     5161 gtacagcagg cgcacgctgt cggcaacata aggcgtttcc tccgccaatt ctcgcagcgc
     5221 acacgctgcc atatcctcgc ccgccacatc cagcttgccc gcaggaagtt ccaatgtcgc
     5281 ctgatttgcc gcataacgcc actgccgcac caaaacgacc ttttcgtcat ccgttaccgc
     5341 caacacgcac gccgcgcccg gatgccggat gacgatgcgc tgcccttcat tgccgttggg
     5401 cagcctgacc ttatccctgc tgatactgac gaaaccgccc tcgtaaatgg tttcgccgcc
     5461 taattttact tccctcaaat ccacttcata tctcctttcc gtttcctatt ctgccgttca
     5521 atcttcacgc ttgccgcgct cgatttccac tccaacctcg cgcacgcctt ccaaaatacc
     5581 cggcttgacg attttcacgt gcacccacac cgcgccgaaa tatcccaaaa ccaaatcggc
     5641 aatatattcc gccaacgctt ccaaaagcag gaaatcctgt tctttcagat gtcggcgcag
     5701 cgtttcgcat acctcggcat aatgcaccgt attggcaata tcgtcgtccg aacccgcttt
     5761 ctcgggaacg ccgatgtcca aatcgacaat cagggtctgc aaccgttcgc gttcccagcc
     5821 gtacacgccg ataagcgtat ccgccttcat gccgtgcaaa aagattttat ccataatccg
     5881 ccccgttttt tgctaaaata accgcaccat tctaagtaaa gtaccgcaaa tgttcaacat
     5941 accggccgtc gccgtttcct atttaatcgg ttcactttct ttcgccgtca tcgtttccaa
     6001 gtattacggg atggacgacc cgcgcaccta cggatcgggc aatcccggcg cgaccaatgt
     6061 tttacgcagc ggcaaaaaaa aggcggccgc gctgacgctc ttgggcgatg ccgccaaagg
     6121 tttggttgcc gttttgcttg cacgcgtgct tcaagaaccg ctcggtttat ccgacagcgc
     6181 aatcgctgcc gtcgcactcg ccgcgctggt cgggcatatg tggccggtgt ttttcggatt
     6241 taagggcggc aaaggcgtgg caacggcatt gggcgtgctt ctggcactct ctcctgcaac
     6301 tgccttggtc tgcgcgttga tttggcttgt gatggcattc ggcttcaaag tatcctccct
     6361 tgccgcgctg gtcgccacaa ccgccgcccc ccttgccgca ctgtttttta tgccccaccc
     6421 ttcttggatt ttcgcaaccc tcgcaatcgc catattggtg ttgctccgcc ataagagcaa
     6481 catcctcaac ctaattaaag gcaaagaagg caaaatcggc gaaaaacgct gattcggcat
     6541 ttgttttcca ttacgtcatg ccgtctgaaa ccatgttccc gcgttcagac ggcatttccg
     6601 cacaaacggc tttcatacat accatgctca gttacagaca cgcattccac gccggcaacc
     6661 acgccgatat gctcaaacac ttcaccttgt ttttagtgct gcaatatttc aaccgcaaag
     6721 acaagcccta ctggtacatc gacacgcacg gcggcgcggg tttgtacaat ctcgaaggca
     6781 gcgaggcgca gaaagtcggc gaataccggc aaggcatcgc cctgctccga caggcgcaaa
     6841 acctgcctgc cgaactctct gactttgccg cgcacataca aaaaatccta ccctcgcccg
     6901 aactttactg cggctctccg tggctggcgc aatcgctgat ccgcatcggc gacaaattgc
     6961 gcctgtttga gctgcaccct gccgactttg tccatctgca aaacaatatg ggcgaagcgg
     7021 gattgggcaa acgcggacaa gtgttgcgcg aagacggtta caaagggctg atttccctgc
     7081 tgccgccccc gccgcgccgc gccgccgtcc tcatcgaccc gccctatgag gaaaaacagg
     7141 actaccggcg tgtaacggaa acgctgaaag ccgctttaaa acggtttgag tccggctgtt
     7201 atctcatttg gtatccctgc ctcagccgcg aagaaagccg caagctgccg gaagaattga
     7261 aaaaactcct gcccgacaac tacctttacg ccgaactgca cgtccacgcg ccgaaaaccg
     7321 acggtttcgg tatgcacggc agcggaatgt tcgtgattaa tcccccttac cttttagccg
     7381 aacaattggc ggcaaacctc cccgccctga cgcggctgct tgcgcaagac gaaggcgcgc
     7441 gctacctttt ggacagcaaa atccgctgac cccgatgccg tctgaaccct tcagacggca
     7501 tcttccgtca aacaaacaaa accgggcgat acacactcca tatcgtccgg ttctgcataa
     7561 aaccagtatt caaaaatcag aacacgcttt tcattacacc cacggtacca accacaccgt
     7621 tcagcagcat cagaccaccg gcagccgtac ccataccttg acggattgcg tcatgcttat
     7681 tggcacgttt ttcacgttcc aaagccaact cctcagaaac atttttacgt tgacggcggt
     7741 gttgcgccag ttccgcacgg ctaattcgcg cttcggcacg gttacgacca gccttgatta
     7801 cggccgaatc gtcgccgata gcatagttgg ctacagaatt ttcagaagcg caggcagcaa
     7861 gcaaaacggc acacacagca acaacggaaa tatttttcaa gtttttcata cctgtcctat
     7921 tatctcaaaa taaagaaaaa actaatccaa ctggagtttt atcggataaa ataaatattg
     7981 ggtgaatttt cgtttgcctt tttgatggcg cgtgccgcat ccatcatttc ttcccggcgt
     8041 tctttacggt attcggcacg ctcggcacgc tcgcttatct gaatggcacg acttttcgcc
     8101 tgtatttccg ctgcagattc ttgagttaca aaacggcggt ctgcgtaagt acatgccgtc
     8161 accaacatcg cagccaccga caacaaaatc agtttcttca ttttcatcta cctatattta
     8221 agtttatttg taaaatatgt tatcactata tatatatata atgtcaatat tttttaaaaa
     8281 taaatttggc atcttgtact tctcgatccg cctgccgcgt tccgacttgc cccgcgccgt
     8341 ttttttccac tacaatccca cgcgatatcc ggcggaacgc accaaaggaa cgccccgcag
     8401 caatctgccg cctttcagac ggcacaaacc tttccgaaac gagcaaacca tggacacact
     8461 gacccgattc ctccccgaac acctgcaaca aaaccaactg cccgaagcac tcggcggcgt
     8521 tttgctgtcc gtcgtttccg cctgtaccga aatcaacgcc aaagtccgcc tcggcgcact
     8581 tgccggcgta ttgggtatgg cgggcacggg caatattcag ggtgaagacc agaaaaaact
     8641 ggatgttatc gccaacaaca ttatgattga cacactcaaa gccaaccctg ccgttgccgg
     8701 tttggcaagc gaggaagaag acactttcgt aagtgccggt gaaaacggac gctatctcgt
     8761 cctattcgac cctttagacg gatcgtccaa tattgatgtc aacatttccg tcggtacgat
     8821 tttctccatt ctcgccaaac ccgaaggcgc attggcaacc gaatcattcc ttcaaacggg
     8881 cagacagcag cttgccgcag gctatgtcct ctacgggcct caaacccagc tcgcattcac
     8941 attcggacac ggcgtgtacg tatttacact caatgccgaa aacgaatttg tgctgaccaa
     9001 agaaaacccg aaagtacccg aaagtaccaa agaattcgcc atcaatatgt ctaaccgccg
     9061 ccactggcta ccccccgttc aacaatacgt cgacgagctc ttggcgggcg aaaccggtac
     9121 gcgcggcaaa aactacaata tgcgctgggt agccagtatg gttgccgaaa tccaccgcat
     9181 cctgatgcgc ggcggcgtgt tcatgtatct gcaagacaaa cgcgacccgt ccaaacccgg
     9241 caaactgcgc ctgatgtacg aagccaaccc tatggccctg atactcgaac aggcgggcgc
     9301 atcggcaagc aacgcatatc aagccatgct cgatatacag cccgaaagcc tgcaccaacg
     9361 cgtcgccgtc attatgggca gcagcgaaga agtggattac ctcaaccgtc tgcattcaaa
     9421 ataggcagag accgtactga taaagaccgg cggcagcgtg aatccgatac tgcgccgata
     9481 tttcaagcat accctccgga aaaacagaaa actgcctttc cgaacgataa aaataccgtc
     9541 tgaaaaattt tcagacggta ttttaaaaat taaagctcaa atttatttgc ccgccgcttt
     9601 ttgcaggatg gcgtacaact cgtctttcag tttcaatttg gcttttttca gctcatcgat
     9661 ggtttccgca ccgctggtaa ccggattgtt ggccagaccg gtaattttat cgtccagctc
     9721 gttatgttcg tcgaacagac gggcgaagcg ggaattttcc tgtttcaatt tggaaatcaa
     9781 atcacggtat tctgggaaca tatcgctttc ctctcttggt ttacggttaa taaagtcaaa
     9841 ttgaatcttt acattaatta taacaaagat aatattttta tccagtgttt tgttacacac
     9901 ggcaacaccg tccgcagaaa accgccccac cgtccgataa aatcgttgac aatcttccgc
     9961 tttacgcata aattttcagc ccttcctact gtatatgaaa ggcacgcccc agtttcccca
    10021 atgtggattt tcagcgcgcg cagtagaggt gccgacccaa attgacagac cctttgcttt
    10081 tgttaatatc ctagaaaatc cagaaattcg cagtactcta ccactcatcg ccaattggcc
    10141 aacctttcca cggctttggg ttaatggtga gttaatcggt ggtagtgata ttatcctaca
    10201 aatgtaccaa tcaggtgagc taaaaccact cattgaagag catagcccaa aggtctgatt
    10261 acttaatatc ttaaaataaa tatcaaaaaa gactgctgat gacattagcg gtcttttttt
    10321 gatggtcaag attgatagtt tggattgaat tatttagcca tattacatac aataatcagc
    10381 atgctttgta tgaattatca atcaaactca aactcaggcg aaggagtgct tgtggctaaa
    10441 acatatttat tgactgcatt gataatgtct atggtaatct ccggatgtca agtcatccat
    10501 gccaatcaag gtaaggttaa tactaattct gctgtcatcg caggtgcaga cgctcacacg
    10561 cctgaacatg taacgggact gaccgaacaa aagcaggtga ttgcaagtga ttttatagta
    10621 gcgtcagcca atccattagc aacacaagct ggctatgata tcttaaagca aggcggtagc
    10681 gctgcagatg cgatggtggc ggtgcagacg acactaagct tggtagagcc acagtcgtca
    10741 ggcttgggcg gtggtgcatt tgtgttgtat tgggacaata ccgccaaaac attgaccaca
    10801 tttgatgggc gtgagacggc accgatgcgt gcgacgccag aattattttt ggataaagat
    10861 ggttaaccat tgaaatttat ggaagcggtg gtcgctcggt aggtacgcct gctatcccta
    10921 aactgacgga gacaatacat cagcgatacg gtgtattgcc ttggggaaaa ttatttgata
    10981 cgccgatcca cttggcaaaa caaggctttg aggtgtcgcc aaggcttgcc atctcggttg
    11041 agcaaaatca gcagcatttg gcacgctatc caaaaacagc cgcttatttt ttgccgaatg
    11101 gtgtgccgct acaagcaggc agcttgctga aaaatttaga atttgctgac agtgttcagg
    11161 cgttagcagc tcaaggtgca aaagctctgc atactggtaa atatgcccaa aatattgttt
    11221 cagttgtcca aaatgctaag gataaccccg gtcaattatc cttgcaaaat ttatctgatt
    11281 atcaagtggt ggaacgcccg cctgtttgtg tgacttatcg tatttatgaa gtatgcggta
    11341 tgggtgcacc aagctcaggt gggattgctg tgggtcagat tttggggatt ttaaatgaat
    11401 tttcacccaa tcgggtgggg tatgatgctg aaggtttgcg tctcttgggc gatgcttcta
    11461 ggcttgcgtt tgcggatcgt gatgtatatc ttgggcgatg cttctaggct tgcgtttgcg
    11521 gatcgtgatg tatatttggg cgaccctgat tttgtaccag tacccattcg ccagttgatt
    11581 tctaaagact acctaaaaca tcgcagccag ctgcttgagc agtcggataa ggcattgcct
    11641 agcgtatctg caggcgattt tattcatgag tgggcgtcct cacaagcgat tgagttacct
    11701 tctaccagtc atatatcaat tgtggataag gcgggcaatg tgctatcaat gacgacttcc
    11761 attgaaaatg cgtttggctc aactttgatg gcaaatggct acctgctgaa taatgagctg
    11821 acagacttta gttttgagcc aataaagcag ggcaaacagg tcgccaatcg tgtcgagcct
    11881 ggcaagagac cacgctcctc aatggcacca accatcgtat ttaaagcagg caagccttat
    11941 atggcaatcg gctctccagg tggtagccgt atcattggct atgtcgctaa gacgattgtg
    12001 gcacatagtg actggaatat ggatatccaa gatgccatca gtgcacccaa tttattaaat
    12061 cgctttggta gctatgaact agaaacgggt acaaccgccg tccagtggca acaaacatta
    12121 aatgatttgg gctataagac tgatgtgcgt gagctaaatt caggtgttca agccattatt
    12181 atcgagccaa gcaggctggt tggtggtgtc gatccacgcc gagaaggtcg agtgacgggt
    12241 gattagaggt gaagcttgta ttttgtgaat ttgaccgcat taagttaatt attaaaatag
    12301 ggggaaattg tgagtaatca aacaaaaata caagccatca agcaagcttc tgagcagatc
    12361 ttggcaattt gtgagacgcc aaacacagca ttacaagcca ttcatctgat tttaagacat
    12421 ggcggggcag gaagaattgt cttggcaagt ggtgtatcag cgggttatgg ctgataaaga
    12481 tgtggttggt gctggttatt tgattgattt tgcacaaact gccgaaaatt taccatttga
    12541 tgtattgcct ttgattagcc ttgtgctaaa taaaggtgat gagacgctaa aaaccggcat
    12601 gttaaataaa ttacctgata atgccaaaga aaatttacgc attatgggct atctgcctta
    12661 ggttttgaag tattttttta tacgaaaaac gctcatgata ccctgattac acaaaggagc
    12721 cgagaatatg ttttcaaaaa gcgttaccct cgcacaatat gaccccgatt tggcagcagc
    12781 cattgcccaa gaagaccgac gccagcaaga ccacgtcgag ctgattgcct ctgaaaacta
    12841 cgtcagctgc gccgtgatgg aggcacaagg ttcgcaattg accaacaaat acgccgaagg
    12901 ctatcccgca aaacgctact acggcggctg cgaatacgtc gatattgtcg aacaattggc
    12961 aatcgaccgc gtaaaagaac tgtttggcgc agcctatgcc aacgttcaac cgcactccgg
    13021 ttcccaagcc aaccaagccg tatatgcttc cgttttaaaa ccgggcgaca ccattttggg
    13081 tatgtctctg gcgcacggcg gacacctgac ccacggcgcg agcgttaata tttccggcaa
    13141 actctacaat gccgttactt atggcttgga tgaaaacgaa gtcctcgatt atgccgaagt
    13201 cgaacgcctc gcgctcgaac acaaacccaa aatgattgtg gcgggcgcgt ctgcctacgc
    13261 gttgcaaatc gactgggcaa aattccgcga aatcgccgat aaagtcggcg catacctctt
    13321 tgtcgacatg gcgcactatg ccggactggt tgccggcggc gaatatccta accccgtgcc
    13381 attctgcgac ttcgtaacca ctaccaccca caaaacccta cgcggccctc gcggcggtgt
    13441 gattttgtgc cgtgacaata cccacgaaaa agcgctgaac tcttccatct tcccaagcct
    13501 gcaaggcggt ccgttgatgc acgttatcgc cgccaaagcc gtagcgttta aagaagcgtt
    13561 gcaacccgag ttcaaacaat acgcaaaaca agtgaaaatc aatgctgccg tcatggcgga
    13621 agagttggtt aaacgcggtt tgcgcatcgt ttccggccgc accgaaagcc acgttttcct
    13681 cgtcgacctg caaccgatga aaatcaccgg caaagccgcc gaagccgctt tgggcaaagc
    13741 gcacatcacc gtcaacaaaa acgccatccc gaacgatccg gaaaaaccgt tcgttacctc
    13801 cggcatccgc atcggttccg ccgccatgac cactcgcggc tttaacgaaa ccgacgctcg
    13861 cgtattgtcg aacttggttg ccgatgtatt ggctaatccc gaagacgaag ccaacctcgc
    13921 caaagtctgc ggacaagtga ctgccttgtg cgacaaatac cccgtttacg gaaactaaac
    13981 gcccgatgtt tccatacaaa atgccgtctg aacattttcc agacggcatt ttttgttttc
    14041 ggtaacattg ctattttttc tcttccagca aaatgaccaa ctttttcaat actgcgattt
    14101 cactatcttt ctgcctgatc aattcatcct ttgcatccag cagcgacctc aatttttcat
    14161 tttcaaacac cagcatttca tcagagccga taatattatg atggttattg gtattgtcgc
    14221 cgatagagaa gaaaaacgat cggttctgct ccgccagcag ctcaaccaca tcgatattga
    14281 aaatctgggc aatctggcgc agcttgtcga gattgatgcc gcttttcccg cgttctattt
    14341 tcgaataccc gttaaccgac atttccaatt tttccgccat ctcttcctgc gtccactgat
    14401 tgacttccct taacgtccga atcttgtcat gcacttccat cctgccctcc aaggttaatt
    14461 ttcaccactc aaacaaccgt aaccggtatc ttcagttgat ggtcggatat tttaaaaata
    14521 aataatataa cacaatagta ttttgataaa aatttaccta acgaggtaca gacatgtatt
    14581 cagaaaacaa gcctaaacgc accgtaaaaa tgaaaaaata ctggcagcct gcaccctttc
    14641 cctgtccctt tctgcctgta cttcgactca agtaacttct tttgtcgagg gactctccga
    14701 gggactcgta gatgtcagcg aagcctttat cacaacaaag gcagaatcat attctgcccc
    14761 gtctcttcag gataagcaaa ttttcataaa tcgcgtctca gaacgagcac aaaaaaatta
    14821 caaagaaaga aggaaaaaga aagaagcaga cttactaaat tcagcatcgt tattaaatga
    14881 atcaacatca gaagccctat ctgcccttaa aaacgctatc gtaattccta aacgccctaa
    14941 acgctccttg ggcaaaaaat ataaaaacgg ttttcatcct gtaaatacgt gtgcggatgt
    15001 ggaagctcgg tgggcaaacg gagcaatgag agatgttagg gacatagaaa ggctaataag
    15061 ccgaaacgtc cctttacgtc gtatcggaga cgccattcaa aacatgtgca tggaaaccaa
    15121 tgtgtccatt aggatgtatc gccaggagtg ccgttcagaa tcgtcagatt tttatgacga
    15181 aatgcttgag catcacgaga atttcatagg atcctccaaa tcattcgcat catcacttct
    15241 tccccttagc gttacaaact ttacaacaga catggcgtgt actcccgaat tttaatctaa
    15301 tcacactcca caactcccta tcaatataac tgctacttct ctgagtagta tcaaccaacc
    15361 cttaacaaag gactaaaaaa tgaaaaaagc actgcttgca ctgactattg ccgccatctc
    15421 cggtactgct atggcccagt tgcccgactt tctgggtaaa ggcgaatata ccgtccgtac
    15481 agacatctcc aaacaaacgc tgaaaaatgc cgatttgaaa gaaaaacaca aagtacaaaa
    15541 aaacatcggt ttccgtgccg atatgccgtt tgacgatatt caccacggca tgcgtttcga
    15601 agtatcacac agccgagaca aaaaagacat gtacgttgtg accgaaagta ctactaaacc
    15661 attcggcaaa gacgttaaag aaaaacgcac cgacgtgtat gccggttaca cctacactca
    15721 accgatcagt gaagccacca aactgcgtgc cggtttaggt ttgggctatg aaaaatacaa
    15781 agatgcggta gctaatgaga aaggaacagt cagcaccgag cgcgaagcct tctataccaa
    15841 agcgcacgcc gacctgactt ccgatttggg cggcggctgg tatctgaatc cttgggcaga
    15901 ggttaaggtt gacctggacg ccaaattgaa acacaacgca actgtagccg gtgtctcagc
    15961 cgacattaac gcaaaaacac gcggatgggg cgtgggcgta ggtgcgaata tcggcaaaca
    16021 aattaccgat accgtcggca tcgaagccgg cccgttctac aaacaccgtc acttcaaagc
    16081 atccggcagc ttcgttttag acggcgggaa catccgagtc gatcctacca aaatcaacga
    16141 atacggtgtt cgtgtcggcg tgaaattcta atcgcggcga tatgccccag acacaaaacc
    16201 gcaagccttt tggtttgcgg tttttgatgt tgtagcagac cctaataccg tctgaaactt
    16261 ttcagacggc atttttccaa tcggttaaaa taccgcctcg acattgtgtt ttggaaaacc
    16321 cgtgcttgct tctgtgattg attttatcct gcatatcgac caacacctgc tcagcctgtc
    16381 ggcgcaatac ggtgtgtgga tttatgcgat tctgtttttg attgtttttt gcgaaaccgg
    16441 tctgattgtt acgcccttcc tgcccggcga ttcgctcttg tttgccgccg gcgggattgc
    16501 cgcgctgggc ggtatggata ttcatttgat ggttgcatta ttaagccttg ccgccatact
    16561 cggcgacgcg ctcaatttta ccgtcggcaa atatttcggc ggcaggctgt tcgccaaccc
    16621 cgattctaaa atcttccggt gcgaatacct cggcaaaacc cgccgttttt acgaaaaaca
    16681 cggcggcaaa acgattatca tcgcgcgctt cgtgcccatc gtccgcacct tcgccccctt
    16741 cgtcgccggt atgggaaaaa tgcactatgc caaatttatc cgctacaaca tcatcggcgg
    16801 cttgttgtgg gttatcttgt tttcttacgc gggctatttc tttgccaact tccccgtcgt
    16861 aaaaaacaat ctgggcttgg tgatgggcgg catcattatt gtttccgtcc tgcccggcat
    16921 catcgaaatc gcccgcgcca aacttgcggc aaaatccgaa cgttaattgt tggaaattga
    16981 cattatgaat atattatccg tagaaaacgc ttcctttgcc gtcggacatg tcgccctgct
    17041 cgacaaaacg tccttccaac tcgacagcgg cgaaaaagtc ggcttgatcg gacgcaacgg
    17101 cgcaggcaag tcttcgtttt taaaaatcct caccggcgtg caaaaactcg acgacgggca
    17161 gattattgtt caaaacaatc tcaaaatcgt ttatgtgccg caggagtcct tttttgataa
    17221 ggacgcaacc gtatttgata ctgttgccga aggtttgggc gaaattcgcg atttattgcg
    17281 ccgttatcat catgtcagcc atgagttgga aaatggttcg agtgagctct tattgaaaga
    17341 gcttaacgaa ttgcaacttg aaatcgaagc gaaggacggt tggaaactgg atgcggcagt
    17401 caagcagact ttgggggaac tcggtttgcc ggaaaacaaa aaaatcggca acctttccgg
    17461 cggtcagaaa aaacgcgtcg ccttagcgca ggcttgggta cagaagcccg atgtattgtt
    17521 actggacgaa ccgaccaacc atttggatat cgacgcgatt atttggttgg aaaacctgct
    17581 caaagcgttt gaaggcagct tggtcgtgat tacccacgac cgccgttttt tggacaatat
    17641 cgccacgcgc attgtcgaac tcgatcgcgg tattttgcgc tcctatcccg gctcgttctc
    17701 caaatacagc gagaaaaaag cgcaagagtt ggcggttgaa gccgaacaca accgcctctt
    17761 cgacaaattc cacgcgcagg aagaggcgtg gatacgcaaa ggcatcgaag cgcgccgtac
    17821 ccgcaacgaa ggccgcgtgc gccgtttgga agaactgcgc cgccagcgtg ccgaacgccg
    17881 caatgtacaa ggacaggtca acttcaaact cgacagcggc aagaaaagcg gcaaaatcat
    17941 cgccgaactg gaacacgctt cgtttgccta tgacgacaaa gttatcatgg ataaattttc
    18001 cgccatcttg cagcgcggcg acaaaatcgg cttaatcggc ccaaacggta tcggcaaaac
    18061 caccttcctt aagctgattt tgggcgaatt gcagccgacc tacggcagaa tccgtatcgg
    18121 cagcaagcag gaagtcgcct atttcgacca gttccgcagc gccttgaatg aaaacgatac
    18181 tgtgttttac acgctcggac agggcaacga ttacgtcgaa gtcggcggca agaaaaagca
    18241 tgtgatgagc tatttggaag atttcctgtt cccccctgcc cgcgcgcaaa gtcctgtttc
    18301 ttcactctcc ggcggcgaac gcaaccgcct tttgctggca aaactcttta ctcgtcccgc
    18361 caatattttg gttttggacg aaccgaccaa cgacttggac atcgacaccc aagaattgct
    18421 tgaagatctg ttgcgcgatt accaaggcac ggtcttcctc gtctcgcacg accgtatgtt
    18481 cctcgacaat gtaattaccc aaagcattgt tttcgaagga caaggccgtc tgaaagaata
    18541 catcggcggc tatcaggact atatcgacgc aaaatcgcgg gaagataaaa ttcagacggc
    18601 atccgcaccc aaagcatccg atgtcgaacc cgccaaagaa aaacccaaag ccaaccgtac
    18661 ggtcaaactt tcctacaaag aacagcgcga actcgacgcc ctgcccgacg aaatcgccgc
    18721 cttggaaacc gaacaggcag aaatcaacgc gcagctttcc gatcccggaa ttttcaaaga
    18781 ttacgaaaaa gcaggagcat tgcaaaaccg cgccgaagaa atcgaaatgc tgcttttgga
    18841 aaagctggaa cgctgggagc tgctggaaac caaacagaac ggcaatgccg tctgaaatca
    18901 aacaggaaga aaaaaaatgt tcaaacgacc tgaagaaatc atcgtcctga tacttgccgt
    18961 cctctggatt gccggtacgt attttctcgc cgccctgttt ggagcggatg cttataccgt
    19021 gttaaaaatc actgccctta ccctgctttg gtctgccgca tcattcctcc tttggcaaaa
    19081 aaaacctcag ccggcatatc tggccgctgc tgctcggctg cctgaccacc tgttggtggc
    19141 cgtatctgaa tcaatcggtc gcacccggtt ctttaccttg gcatgcatca tggacgttca
    19201 aaaccatctt agccctgatt cccgcaaccg caggttatct gtttaaatgg aaacaatacc
    19261 ggcagcgcaa aacaaagccg taagcccgtc ttttttcaga cgccatctcc ggtcagactg
    19321 tcaacaattc cgataaaatg ttatactcgc gcccgttgcc gcgccgtccg aaaccgcctt
    19381 ttgggaaagc agaaatgatc agcaaacaag aataccaagc ccaagccgcc caaggctaca
    19441 accgcatccc gctcgtgcaa gaactccttg ccgacttgga tacgccgctt tccctctatc
    19501 tcaaactcgc caaccgcccc tatacctacc tgctcgaatc cgttgtcggc ggcgaacgtt
    19561 tcggccgcta ttcctttatc ggcctgcctt gcagccacta tctcaaagcc ggcggcaaac
    19621 acgtcgatgt ttatcaaaac ggcgaaatcg tcgaacaata cgacggcaat ccgctaccct
    19681 ttatcgaagc tttccacaac cgtttcaaaa cacccgaaat cccaagcctg ccgcgcttta
    19741 ccggcggctt ggtcggctac ttcggttacg aaaccgtcta caatttcgaa cacttcgccc
    19801 accgcctgaa aaacaccgcc aaagccaacc cgctcggcac gcccgatatt ttgctgatgc
    19861 tgtcgcaaga gttggcggta atcgacaatt tgagcggcaa aatccacctc atcgtttatg
    19921 ctgatccctc gcagcccgac agctacgaac gcgcccgcga acgcctcgaa gacatccgca
    19981 cccagttgcg ccaaagctgc gccatcccac tttccctcgg cagcaaacaa acccaagcag
    20041 tcagcgagtt cggcgaagaa cctttcaaag cctgcgtcga taaaatcaaa gactacattt
    20101 tcgcaggcga ctgtatgcag gtcgtcccca gccaacgcat gagcatggaa tttaccgaca
    20161 atccgcttgc cctctaccgc gccctgcgca cgctcaaccc ttcgccttac ctcttttact
    20221 acgatttcgg cgatttccac atcgtcggtt cctcgcccga aatcctcgtc cgccgcgaac
    20281 gcgacgacgt catcgtccgc cccatcgccg gcacgcgcct gcgcggcaaa acccccgccg
    20341 aagaccttgc caacgaacaa gatttgttaa gcgacgccaa agaaatcgcc gaacacgtta
    20401 tgcttatcga tttggggcgc aacgatgtcg gacgcatcag caaaacaggc gaagtcaaag
    20461 tcaccgacaa aatggtgatt gaaaaatact cccatgtgat gcacatcgtt tccaacgtcg
    20521 aaggctgtct gaaagagggc gttaccaata tggacatcct cgccgccacc ttccctgccg
    20581 gcacactctc cggcgcgcca aaagtccgcg cgatggaaat catcgaagaa atcgaaccgg
    20641 agaaacgcgg catctacggc ggcgccgtcg gcgtatgggg cttcaacaac gatatggatt
    20701 tggcaatcgc catccgcacc gccgtgatta aaaacaatac cctcttcatc caaagcggcg
    20761 cgggcgtggt tgccgactcc gacccaactt ccgaatggca ggaaactcaa aacaaagccc
    20821 gcgcagtggt tcgggcggcg cagatggtgc aggaagggtt ggataaataa atttcagaca
    20881 acctttgcag atgaaaagaa ggtcgtctga aaatcacaaa acaacatcag agatacttac
    20941 ataaaaaatc acaatgcaat tcacatcatt agaaatttgt gccggagcag gcggacaggc
    21001 tttgggctta gaaagggcag gcttttccca tgttgcgtta atcgaaatcg aaccgtcggc
    21061 ttgtcaaacc ctacgtttaa accgtcctga ttggaacgtt attgaaggag atgtccgctt
    21121 gtttcagggc gaaggatatg acggtattga tttattggcc ggcggcgtgc cttgccctcc
    21181 tttttccaaa gctggaaaac aactgggaaa agatgatgaa cgcgatttat tccctgaggc
    21241 tattcgtcta gctaaggaaa ctgaccctaa agccatcatg ttagaaaatg tgcgggggtt
    21301 acttgaccca aaatttgaaa attatcgcaa ccatattaca gaacaatttg ccaagttggg
    21361 ttaccttgga caatggaaac tactttatgc tgccgattac ggagtatcgc aactcagacc
    21421 cagggtttta tttgttgcgt tgaaaaacga atacaccaat tttttcaaat ggcctgaacc
    21481 aaattcggaa caaccgaaaa ccgttggaga actgcttttt gacttaatgt cagaaaataa
    21541 ctggcagggg gcgcataact ggcgtttgaa ggccgcgcag attgcaccga ccttggtcgg
    21601 cggttcaaaa aaacacggcg gtgctgactt ggggcctaca cgatccaaac gcgcatgggc
    21661 ggagttgggt gtggatggtt caggtttgtg ggatagtgcg ccgcctgaag acttttcggg
    21721 aatgcctaga ttgactgtcc gaatgacggc gcgtatccaa ggatttcctg ataactggca
    21781 attttttggt aaaaaaacgc ctatgtaccg ccaaatcggc aacgcttttc ccccgccggt
    21841 agccgaagcg gtgggcaggc agattatcaa agccttaaaa aaggaaaatt gaatgaatcc
    21901 gctttttacc caagaacgca ggattttcca taagaaatta ttggatggca atattcttgc
    21961 aaccaacaat agaggcgtag tcagcaatgc ggatgggagc aatacccgaa gtttcaatat
    22021 agccaagggg attgccgact tactgcattc ggaaacggtt tcggaaagat tacccggtca
    22081 gacatcggga aatgcttttg aagcaatatg cagtgagttt gtccaatctg cttttgaaaa
    22141 attgcagcat atcagacccg gcgactggaa tgttaagcag gtcggttctc gcaaccgtct
    22201 ggaaatcgca cgttatcagc aatacgcaca tctgacagcc ttagccaagg ctgccgaaga
    22261 aaaccccgaa ttggctgccg ccttgggcag tgattacacc attactcccg atattatcgt
    22321 taccagaaat ttgattgcag atgcagaaat caaccgtaat gaatttttag ttgatgaaaa
    22381 tattgccaca tatgccagtt tgcgtgcggg taatggcaat atgccgctgc tccacgccag
    22441 tatctcttgt aaatggacga ttcgcagcga cagggctcaa aatgcccgtt ctgaaggatt
    22501 gaatttggtt agaaacagaa aaggcagact gccgcacatt gttgtcgtaa cggcagaacc
    22561 aacgccaagc cgcatttcat ctattgcctt gggtacaggg gaaatagatt gcgtttacca
    22621 ttttgcctta tatgagctgg aacaaatttt acaatcattg aattatgaag acgctttgga
    22681 tttgttttac atcatggtca atggtaaacg attaaaagat atttccgacc ttcccttaga
    22741 tttggcagtt taaacagaat tattccacta tgaacacacc ccccatcctt cctcccgcca
    22801 tgctcggcat cctcggcggc ggacaattag gcagaatgtt tgccgttgcc gctaaaacca
    22861 tgggctacaa agtaaccgtt ctcgatcccg acccgaatgc gccggcggcg gaatttgccg
    22921 accgccattt gtgcgcgccg tttgacgacc gggccgcgtt ggacgaattg gcaaaatgcg
    22981 cggcggttac gaccgaattt gaaaacgtca atgccgacgc gatgcgctct ctggcaaagc
    23041 ataccaacgt ttcccccagc ggcgactgcg tgtccattgc acaaaaccgc attcaggaaa
    23101 aagcgtggat acgcaaagca ggcttgcaaa ccgcgccgta tcaggcggtt tgcaaggccg
    23161 aagacattac tgaagcaagc gcgcaatttt tgcccggcat cctgaaaacg gctacgttgg
    23221 gctacgacgg caaaggtcaa atccgcgtca aaacgttgga cgaactcaaa gccgcgtttg
    23281 ccgaacacgg cggcgtggat tgcgttttgg aaaaaatggt ggacttgcgc ggcgagattt
    23341 ccgtgatcgt atgccgtctg aacgatgaaa acgtgcaaac cttcgacccc gccgaaaaca
    23401 tccacgaaaa cggcatcttg gcttattcca tcgtccccgc gcggctgagt gccgacgtgc
    23461 agcaacaggc gcggcagacg gcgcaacgct tggcggacga attggattat gtcggcgtat
    23521 tggcggtaga aatgtttgtt gtcggcgaca cacatgaatt gctcgtcaat gaaaccgccc
    23581 cgcgcacgca caattccggc caccatacga tagatgcctg cgccgcagac cagttccaac
    23641 agcaggtgcg cattatgtgc aacctgccgc ccgccgacac caaattatta tccccttgct
    23701 gtatggcgaa tattttgggc gacgtttggc aggaagatgg cggcgaaccg gattggctgc
    23761 cgttgcaaag ccggccgaat gcacacctgc acctatacgg aaaaaaaacc gcacagaaag
    23821 gtcggaaaat gggacacttt accgttttga ccaccgattc ggacaccgca tttcaagaag
    23881 caaaaaaact gcatcagtcc ctataatccg accaacggca cagatgccgt ctgaagccgc
    23941 cgacataccg ccatgagcct gctgacactg ttacgccccg ccaccgtgca agactgcaaa
    24001 gacatcttca aagtccacct gcactccgtc caatacacct gcatcctcag ctacaacgaa
    24061 cacgccctaa aagtatggga aggcttgtta aacacagaaa gctacctgcc gaccatttcc
    24121 gatcccgaca aagcactttg ggttgccgaa tacaaaggca acatacaggg atttttccaa
    24181 atcgactgcc aagaagcaca actcgatgcg ctttacgtcc accccctgtt ccacaatctc
    24241 ggactgggca ctgccctgct ccgtcaggca gaaaccatcg cccacaaatc cggtttgagc
    24301 tttctgaaac tctacgcatc tctcaactcc gtcccgtttt accttctcaa ccgctacgaa
    24361 tcgctcggtt cggcagtatt gcagcttgac cccagcatca aaatcaaatg cgaactgatg
    24421 cgcaaacacc tctaatcccg ccttataacc aaaactgata atcttcaaat caaacattat
    24481 ttccacaagc gcagacagcc gtataaagtt gccccatcta ccaaccgaca ggacaagcat
    24541 atgaaaacct acgcacaggc actctatacc gcagccctgc ttaccgcctg cagccccgca
    24601 gccgattcaa accatccgtc cggacaaaat gccccggcca ataccgaatc cgacggaaaa
    24661 aacattaccc tgctcaatgc ctcatacgat gtgacacggt atttttacaa agaatacaac
    24721 cacttattta tcaaaacata ccaatccgaa caccccggca catccgtcag catccaacaa
    24781 tcccacggcg gcttcagcaa acaggcatta tccgtagcca acggccttca agccgatgtc
    24841 gtaaccatga accaatcttc cgacatcgac ctgctcgaaa aaaaggactg gtagaaaaag
    24901 gctggcaaca agccctcccc gatcacgccg caccctacac cagcactatg gttttccttg
    24961 tccgaaaaaa caaccccaaa cagatccgcg attggaacga ccttgccaaa gacggcgcta
    25021 acatcgtcat cgccaagacc tcgggcaacg gacgctacgc cttcctcggc gcatacggtt
    25081 acggtctgaa agccaacaac ggcaacgagc aggaagccca aaaactcgtc gcatccatcc
    25141 tcaaaaacac acccgttttt gaaaacggcg gacgcgccgc cgccaccacc ttcacacaac
    25201 gcaacatcgg cgacgtactc atcacttttg aaaacgaagc caactacgtc agcaaaaaac
    25261 tgacccaagg acagtttgaa atcgtctatc cgagctacac catttccgcc gaaagccccg
    25321 ttgccgtcgt caacagcgtc gtcgcgaaaa aaggcacgca aaaaaccgcc cgcgcctatc
    25381 tcgaatacct ttggagcgaa ccggcgcaag aactcgccgc ctcactctac ctgcgtcccc
    25441 gcaaccccga agtattggca cgacacaaag ccgacttccc cgacttagac accttccctc
    25501 ccgaagaaaa attcggcgga tgggacaaca tcatgaaaac ctacttcgcc gacggcggcg
    25561 tattcgaccg cctgaccgca caaaaatgac cattatgccg tctgaaccgc ctttcagacg
    25621 gcatttcaca catcaaaaca gaaaaccgca cccaaaatca tcatcacaag acacaacggc
    25681 aaaccggata caaaacccaa caccgccgcc aaaatgccgt ctgaagccgt cggcgcaacc
    25741 atccttatct ggggcgcgcc tcatcaaagg ggcgcataac tttccttaac catccctttt
    25801 gacagccaaa taaacagtgc ttttgcctgt cagaaacaaa accaatattt tgcggttcgt
    25861 atcggaacat ttataaaaca tcagatttcc gtgtttcgcg tttccggcaa ttcaagatta
    25921 gcaaaaccat taacctgctt cgcaaggcag gtttttaaaa ctttatcagg cgttcgagca
    25981 tatcggttcg accgacccgc atttccggca atcaacactg ccgacggttg ccgcatttct
    26041 ttttgatata caatcacact ttcaaaaaac atacgacggc aacaaaatca tcctcccccc
    26101 gcttggcaaa catccggcaa attaaaaccc cgcccgaaac agccatcgga caaataacag
    26161 gaaaaaatat gaataccgac ttccaaaaga tcctcaaaca actgggcaaa caggccaaaa
    26221 aagaagcggc agaaaaacag gaagccgaaa aaaacaagca aaaacaagaa tatgatttgg
    26281 atttttcaca agcagtcgga caagtctccc cattaaaaaa ccggcagcaa tattatgcgc
    26341 agtccgacaa aacccccatc aaagcccgtc ccaaagacaa ccgcaccgac gaagaaaact
    26401 acttctacat aggcagcaca tacaacgacc cacccgccag cttctgcaaa aacggacagg
    26461 gaaaaaacga catccaacgc ctgaaaaacg gatactatcc cgtcgttacc gatgtcgacc
    26521 tgcacggcta cacccaagaa gaagcacaaa aagtcctcaa cgaattcatc gcattcaccc
    26581 aaaaacgcgg cgtatgcggc gaaatcatcc acggcagcgg attaggttcc aaaggctaca
    26641 aacccgttct gaaaaacatg acccgaaact ggctgatgca acaccccgac gtactcgcct
    26701 acgtcgaacc ccgggaaggc aacgacggct gcgtccgcat cctgctcaaa cgcaaattaa
    26761 ggcagcaaga ctgacaaatt tctccgacag acttggcagc accgcacaat atccgtataa
    26821 tttgcaaacc atttctgggg gcgaccttgg tttcgacggg ggttgcgaag cagatgcggg
    26881 cataccgggg tctcagattc ccgtaaaaca ctgaattcaa atagtcgcaa acgacgaaac
    26941 ttacgcttta gccgcttaag gctagccgtt gcagcagtcg gtcaatgggc tgtgtggcga
    27001 aagccaccgc aacgtcatct tacattgact ggtttccagc cgggttactt ggcaggaaat
    27061 aagacttaag gtaactggtt tccaaaaggc ctgttggtcg gcatgatgga aataagattt
    27121 tcaaatagac acaactaagt atgtagaacg ctttgtagag gactttcgga cgggggttcg
    27181 attccccccg cctccaccat gccctatttt cagcagtatc ctgcaacaca aaaaatcctt
    27241 gaacttcaaa agtttcaagg cttttttatt gccttgaata tgatggcgta tgcttgagtt
    27301 ttcgcaattt atggggtaac ttttggggta acttttcagg aatcacccta aaaaatatct
    27361 taaaaataca aaagccatca cgcatttttt aaaaaggtta cccaaaactt aaatgaagcc
    27421 tttatttata aggcttgcag ctttccaaac aagttacccc caaacggata aaaaatgccg
    27481 aag
//
LOCUS       DAXDZG010000020        27087 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXDZG010000020 DAXDZG010000000
VERSION     DAXDZG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27087
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.020"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            279..725
                     /gene="yciA"
                     /locus_tag="ACFB78_000773"
     CDS             279..725
                     /gene="yciA"
                     /locus_tag="ACFB78_000773"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC1268810.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(776..1597)
                     /locus_tag="ACFB78_000774"
     CDS             complement(776..1597)
                     /locus_tag="ACFB78_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1268811.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENVLNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1716..2174
                     /locus_tag="ACFB78_000775"
     CDS             1716..2174
                     /locus_tag="ACFB78_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC1268812.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2317..3453)
                     /gene="lst"
                     /locus_tag="ACFB78_000776"
     CDS             complement(2317..3453)
                     /gene="lst"
                     /locus_tag="ACFB78_000776"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC1268813.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            3590..3730
                     /locus_tag="ACFB78_000777"
     CDS             3590..3730
                     /locus_tag="ACFB78_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC1268814.1"
                     /translation="MPPHIRHNAADAAAKRGKAISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3798..6023
                     /locus_tag="ACFB78_000778"
     CDS             3798..6023
                     /locus_tag="ACFB78_000778"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC1268815.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEITAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6320..6622
                     /locus_tag="ACFB78_000779"
     CDS             6320..6622
                     /locus_tag="ACFB78_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268816.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RAETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
     gene            7581..7748
                     /locus_tag="ACFB78_000780"
     CDS             7581..7748
                     /locus_tag="ACFB78_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268817.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            7908..8759
                     /locus_tag="ACFB78_000781"
     CDS             7908..8759
                     /locus_tag="ACFB78_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC1268818.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGCQVGPKTTADDRTGLRRAVAALAGRKGIDYSSAYSMVHQRFNVESIEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALADIAAMVYYGTRMIESG
                     KDVSAPLKQLGCRQAVTMWTVWHETRSILKRSAAALEVLRGYANKDASDRIAACLEGI
                     YSKATVR"
     gene            complement(8796..8945)
                     /locus_tag="ACFB78_000782"
     CDS             complement(8796..8945)
                     /locus_tag="ACFB78_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268819.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(8948..9622)
                     /locus_tag="ACFB78_000783"
     CDS             complement(8948..9622)
                     /locus_tag="ACFB78_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268820.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(9619..10104)
                     /locus_tag="ACFB78_000784"
     CDS             complement(9619..10104)
                     /locus_tag="ACFB78_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268821.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(10110..10583)
                     /locus_tag="ACFB78_000785"
     CDS             complement(10110..10583)
                     /locus_tag="ACFB78_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268822.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(10638..11930)
                     /locus_tag="ACFB78_000786"
     CDS             complement(10638..11930)
                     /locus_tag="ACFB78_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC1268823.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGARG
                     FHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTDL
                     FTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQL
                     QASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESGL
                     KIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGTI
                     RGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(12556..19857)
                     /locus_tag="ACFB78_000787"
     CDS             complement(12556..19857)
                     /locus_tag="ACFB78_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC1268824.1"
                     /translation="MSDLVRYDPLEHGRLVGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMAGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAAVAAAKGTPEFAALAKESAKG
                     GLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGFAGTK
                     AYAAVTDPLRPPAGRKAGAEGGIGGNRKAGRPAPEGAQALRAAADGGAEQGGAGFDTA
                     HHDQSHPALRQFADRTKQEEAGRFFSGPADGNTPRAEELARGTEKQPDVSGIPSEDGA
                     EFLDTGVMPDGLARQYADMAAEYRAKPSEAMGINPDDGAVSAAAALAADSGAAVPSAV
                     SDDMEARSVADDVPSGRSADADRGGVPSAYGNVRPGGAPRGAASVAPGGSAAAASGGI
                     ARVSPLPAGQYFDGLDTRGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQAYHARIEA
                     DYQAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALDKESRRRFDALPEWVRRHAQT
                     VADYTADGIMRREAGMADMRGHYPEGLAESAGAVRAYRAQHPESADVLDRLNRAVYGY
                     RRNNGWSVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDKGLAQDVRQ
                     DVRQGLTQGGRGLTPDAGADANAAALQGLPGSAVASGNAPARRQNLQVRARAEGAAPG
                     LSASENLAGTDGGKRAPVAGKRPDTVLPVLNPQVAESAGRVSPKKRMADAAADFTRRL
                     AADRRRPEKAGVPLGGGEYRFEHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNSD
                     GLVSDGRRYLKGREAETLRAGGLSEAVPSEPGRDYRPTQEARAPAKVMARPRDAASAG
                     KPAGRAQPARAKDAPVAGKAAAAKNAATEKPSSDKVRNIEAGKSHFDGGKGKSAAAQG
                     AATEKPSSDKAAEPETFAKTALDNPEEARRKARVLQGGPVYTVKGRQAPQGFKALREH
                     AENIKKRLAESIGGLAERVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAENL
                     TPERAVWAAWHELGHRGFAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAAA
                     SVRPAAVEEAVAELYAAQRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVLQ
                     RLAGKAGGAMSDADVFAMLADLHGNVEGARDAPWGGNHRAVMFARAEDGAAERSKSES
                     LEKLRRAETIRISGREVPEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRSGIT
                     EILRHDYKDAEHLQSIAAIPQIIENAVYIDTLPNEDLAKNGDIQGYEYYVSGLNVGGA
                     DYTVRAAVAVSRNGNRYYDHKLTKIEKGNLLSLLDRVSTTGASESKSPLSGIDDKRLL
                     QILQDKDAGKGGIADFDTEAVRFSRAANIGAAIGHITGKKSDLRNALKDRWDASKGIQ
                     LQFLGRRQIEDIYGGVLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAKA
                     LADLMHDATLAKVDADPLMRKDARGRLDGIRTALDIADGKIAKAEAAVASAGARIARA
                     DAAVRKAQRAADKAAYALEKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGAD
                     AAAESRAKTDAVRMSEQARADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVYR
                     KARDDYRAHFGQVRDALAERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLVV
                     VKDADGNSANVSRAETLSEAEKLRDALKADFGAGFKVSPVMKSRDYIRSRDAVGSGFM
                     KELGEAVGRMDLDPAQRARLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRAY
                     AQNMGSGANYLAKLRYADRMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHEA
                     VMNPNPSKLAQALTGFGFLWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAARELL
                     RASKQIGLRFGEKFNTIEDSLNEDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLAG
                     SAWQKVMDKAAWLFHHAEKFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDWHFDY
                     AAQNRPRFMMGNAAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAM
                     ASGILGLPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADAFGDKAGEVLAKGFSRLT
                     PLDVSGRLGLNQLVFPDIQDGLEGKKWAESLVVGSTGAVVGAGIGAADGVRTRSSVPR
                     TANTLSLMKSW"
     gene            complement(19854..21050)
                     /locus_tag="ACFB78_000788"
     CDS             complement(19854..21050)
                     /locus_tag="ACFB78_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268825.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPYAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQKGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAALGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(21287..23554)
                     /locus_tag="ACFB78_000789"
     CDS             complement(21287..23554)
                     /locus_tag="ACFB78_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268826.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVGEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGALLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFP
                     YVPFFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQ
                     FRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPS
                     FMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTV
                     VIEGDAVTQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGA
                     MSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIAQAVQD
                     ALAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAGGQIAAMPQIAP
                     VADAVMQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSAN
                     HAQTGMETPTVSDNL"
     gene            complement(23539..24813)
                     /locus_tag="ACFB78_000790"
     CDS             complement(23539..24813)
                     /locus_tag="ACFB78_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC1268827.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCAREIQKSMRDSVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     DLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     AAGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(24794..25036)
                     /locus_tag="ACFB78_000791"
     CDS             complement(24794..25036)
                     /locus_tag="ACFB78_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268828.1"
                     /translation="MAENLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIE
                     RKSYGMDTAESKISESAKTAGIRIEFVGPEDDGKDG"
     gene            complement(25091..25333)
                     /locus_tag="ACFB78_000792"
     CDS             complement(25091..25333)
                     /locus_tag="ACFB78_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268829.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLK"
     gene            complement(25333..25755)
                     /locus_tag="ACFB78_000793"
     CDS             complement(25333..25755)
                     /locus_tag="ACFB78_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268830.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDF"
     gene            complement(25820..26338)
                     /locus_tag="ACFB78_000794"
     CDS             complement(25820..26338)
                     /locus_tag="ACFB78_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1268831.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(26329..26712)
                     /locus_tag="ACFB78_000795"
     CDS             complement(26329..26712)
                     /locus_tag="ACFB78_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFC1268832.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SIEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(26709..27014)
                     /locus_tag="ACFB78_000796"
     CDS             complement(26709..27014)
                     /locus_tag="ACFB78_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFC1268833.1"
                     /translation="MSSAIRKAAKGGQCTPNIAGVCNDNPETVVLCRFPGETHGAGLK
                     SGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRRLEALGVVGVKGRLK"
BASE COUNT         6086 a         9003 c         6220 g         5778 t
ORIGIN      
        1 atgcggaatc tagagaaacc ttttaaccga taagtttccg tgcggacagg tctggattcc
       61 cgcctgcgcg ggaatgacgg ttcggttgct tagggtagga tttggcggga ttggtgggct
      121 gaagcccgcc ctacgcatcc gccctacaat actccttgcg aatctgtccc gcgcccgacc
      181 ttgtatgtct caaatgggtt aaactacgca cttcccgact tcccgtttcg ggcaggggtg
      241 caggtccgat ttatatttca ataaaacaag gaaactttat gcagcacgaa gaaggcaacc
      301 gccaacgccc tcaaggcgaa ctgctcctgc gtaccgtcgc tatgccgcgc gataccaatc
      361 ccaaccaaga cattttcggc ggctggatta tgtcgcaaat ggatttgggc ggcggcatat
      421 tggcggcgga aatcgcgcgg gggcgcatcg ttaccgtcgc cgttcaggaa atgaacttca
      481 tccgcccggt caaggtcggc aacgtcgtct gctgctacgg gcattgcgtc cgcgtgggca
      541 atacttccct ccagcttaaa gtcgaagtct gggtgaaaac tttgatgaac gattgcgtta
      601 ccgaagaccg ccatctcgta accgaggcgg tgttcactta tgtcgccatc gatgcggaag
      661 gcaatccgcg cccgattccg aaagaaggca accccaaatt gtcaggctta ttggccaccc
      721 cgtaagaata cccgtaaaaa tgccgtctga aactgtcttc agacggcatt ccgtatcatt
      781 tgaaccaaga cctgatgcgg gaaaacagcg acatactctg tttttcaaag tcttcccgca
      841 cttcgggcac ggctgttttt atgaccttca ttcctgcaaa agtctgcgcc acaggcataa
      901 tttcaatcgt gttcacattc atatgcgccg gccggcggta cagccacaat gcggtttccg
      961 caatatcttc ggggcggata aattccacac cctcatacac gcccgccacc ctctcgttat
     1021 cgcctttgaa gcgcacgttg gaaaactccg tattgccgca caaacccggc tcgatattgg
     1081 taacgcggat gttcttatcc gccagctctg cgcgcaaatt caggctaaac tggcgcacaa
     1141 acgccttggt cgccccgtaa acgttgctgc cggcataagc ataattgcct gcaatcgaac
     1201 ccaaattcat cacataaccg ccgccgcgtt ccaccatttg cggcaaaatc ttgcgcgtca
     1261 ggaacgtcaa acccaaaaca ttggtttgaa tcattgtttc ccaatcttca aaatccgcct
     1321 tgtccgccgt gtccaaaccc aaagccagcc cggcattgtt gatgaggcag tcgatgtcgg
     1381 aaaattcatc ggggatgccg ttcaagacgt tttccaccga ctcgcggcac gacacgtcca
     1441 tttccaaagg gtaaaacaaa gcacccaatt catccgccaa agcctgaagc ctgtccgcac
     1501 ggcgcgccgc accgataacg cggtatcccg ccccgacaaa cgtgcggcac atcgcttcgc
     1561 cgaagcctgc cgaagcaccg gtaattaaaa cagccattgt ttttcctttc tttctcgttt
     1621 cccaaacctg tttcaggtat cataacaccc ttcagacggc atcgcgccgg ctgaaaacga
     1681 caacaaccgt accaccacga caggacacat tcaacatgaa aacccaaatc agccttgccg
     1741 caaccgccct cgccctattc ctttccgcct gcggaaacgg cggcgcgccc gcacagccca
     1801 aaggcgaaat ttccgaaaac cgcaccgccg cgttcaaatc tatgatgccc gacttctcgc
     1861 gtatgggcaa aatggtcaaa ggcgaagagc cttacgatgt cgaaaaattc aaacaggcgg
     1921 cggcggcgtt tgccgaaagc agcaaaaaac cgttcacact ttttgagtcc gatccgcaag
     1981 gcaacggccg cgccctgcct gccgtttggt cggatggtgc aaaatttgaa gccgaaaaaa
     2041 caaaattcgc cgccgccgtc gagaaactca acgccgccgc ccaaaccggc aaactggacg
     2101 aaatcaaagc cgcctacggg gaaaccggcg caagctgcaa atcctgccac gacagtttcc
     2161 gcgcaccgga ataacatccg aaacaaaaca atgccgtctg aagccttcag acggcatttt
     2221 ctttgtcatt tcggcacacg caaagcaatc ggaaattatc ttcaattcaa aaaatacgcc
     2281 atatcctgat ttttgcctta cttactccgc cattttctat tttgatttac catgcgattg
     2341 atttttatcg tcaaatgcca aaatcggaat gtcggcctga cggaacagcg cataaacggg
     2401 cttgagccaa taatcttccg gaagggaagc cggtttcaat gcgtaaacgt gcacattggg
     2461 aaaatccttc atcgtcaacg ccgcaccgct gaaaaaggta taaatttcat accttgtatg
     2521 cgggtttttc ttaatttcgc gcaaaatata gtcttcgatg acatagggcg aatttaacgc
     2581 ggttacgccg gaaagcccgt aggtctggcg gggatgcggc gcgacatatt gtatgttgaa
     2641 attttttgcc gccttttccg aaatttcctt catctctttg tcgggcgaac ccaaaagtat
     2701 ccgcaccgtg ccgcccgttt cgtcccccgc cttcagttcg gacgcatcga acagcggcag
     2761 gtaagtcatc ttgcggcggc cgtcgtccat aatgtttttc aaacccttga atatcgtgta
     2821 atgctcgtcg gaagcattgc gggttttggc gatgctccaa tccccgacca tcattcgggc
     2881 aaaattccgc ttaatcgccc cgtttacata aaactcatcg cccaaatagc tgctctcccg
     2941 tatcaggttg tttgtgccgt cgtcaaaggt cttgatttcc gcatccgggt aagtgctcaa
     3001 aaaggcggcg atgctgactt tttccaaact cgccaaataa atccgcctga ccttcggcag
     3061 cagcatcgac ttcaccttca gctccgccat cgtcggaatg aaattaaacg atttgttcaa
     3121 accgtagggc aggtagaaaa aatacgcccg ctccgcctta tcctttatct gattgaaata
     3181 ataatcgtat ttttcattcc tgttttcaga catcagcacc acataaaacc gctcccccgg
     3241 atgctgcgcc ataatccttt ctgccacctt catctgcaat atggtatagc agaaaatcag
     3301 attgacgggt ttcccctctt cattgaagag tttgtccttc agcagggaaa ccgcgttcct
     3361 ttccccctga tttacccggt caaacgtata aaatatcccg aagcaaaaaa caatcaggca
     3421 caacacggtc aaacagactt ttttcaaccc catatcccta aaaactccat tccgacaaat
     3481 tgaacatacg ccccgagtgt cccggtgcgg aaactccggt gcggaaaatg cggaatttta
     3541 gcagatattc cgacagttgc cgaatattaa aattaatttt accgaaaatg tgccgccgca
     3601 catcaggcac aacgccgccg acgccgccgc caaacgcggg aaagccattt cccccttcct
     3661 ttcccgccgc cgcgttcaaa cacgccaaga tcgaacaaag cgcgaacaaa gccgccaaaa
     3721 tatgccataa tagccgcgca cattcccccg cgcaatgtgt ttcaaaagaa aaaaccaacc
     3781 tacaagagaa taatcctatg actcaaaaat ccaccattgt ttatacccat accgacgaag
     3841 cccccgcgct ggcgacccaa tcgctgctgc cgattgtgca ggcgtttgcc cgccacgccg
     3901 atattgatgt caaaactgcc gacatttccc tgtccggccg cattttggcg gcattccccg
     3961 aatatctgac cgaagcgcag cgcgtacccg acgcgcttgc cgaattgggc gaactggtga
     4021 aacaacccgg tgcaaacgta atcaaactgc cgaacatcag cgcatccgta cctcaactga
     4081 ccgccgcgat taaagaattg cagtctaaag gctttgccgt tcccgactat cctgccgacc
     4141 ctcaaaccga tgaagaaaaa gccgtacgcg aacgctacga ccgcatcaaa ggcagcgcgg
     4201 taaaccctgt cctgcgcgaa ggcaactccg accgccgcgc gcctaaagcg gtgaaaaact
     4261 ttgccaaaaa aaatccgcac agcatgggcg cgtgggcgaa agactccaaa acccacgttg
     4321 ccaccatgca aagcggcgac tttttccata acgaacaatc cgttaccgta cccgatgcga
     4381 cttccgtatc catcgtgttc accgacaaac aaggcaataa aaaagagctg cgcgagcctg
     4441 ttgccctgaa agccggcgaa atcattgacg cggccgtaat gagcaaaaaa gccctgctcg
     4501 ccttccttgc cgaacaagtg aaagacgcaa aagctaaagg cgtattgttc tcgctgcata
     4561 tgaaagccac gatgatgaaa gtgtccgacc cgattatctt cggacacgcc gtcaaagtgt
     4621 tcttcgcgcc tgtatttgaa aaattcggcg gcaaactggc tgccgcaggc gtcaacgtca
     4681 acaacggctt cggcaacctg attgccaatc tggacaaact ggatgcggac acccgcgccg
     4741 ccgtcgaagc tgaaatcacc gccgtttacg ccgccaaccc ggatttggcg atggtcgatt
     4801 ccgacaaagg catcaccaac ctgcacgttc ccagcgatgt catcgttgat gcttctatgc
     4861 ctgcgatgat ccgcaattcc ggccgtatgt gggacaaaaa cggcaaagcg caagacacca
     4921 aagccgtgat tcccgacagc agctatgccg gtgtttacca agcaaccatc gatttctgcc
     4981 gcgaacacgg cgcattcgat ccgacaacaa tgggtactgt gcccaacgtc ggactgatgg
     5041 cgcaagcggc ggaagaatac ggctcacaca acaaaacctt tgaaatcgaa gccgacggtc
     5101 aggttcaagt gattgatgcg gcgggcaatg tcctgatgca gcacgacgtt gaggcaggcg
     5161 gcatctggcg catgtgccaa accaaagacg ctcccgtcaa agactgggta caactcgccg
     5221 tcaaccgcgc ccgcctgagc aacacgcccg ccgtgttctg gctcgacgaa aaccgtccgc
     5281 acgacaagag tctgctcgcc aaagtcaaag cctaccttgc cgaattggat accaacggcc
     5341 tcgacatccg cgtcctcgcg cccgaagaag ccgctaaatt cagcttgggc cgtctgaaaa
     5401 acggcgaaga caccatttcc gtaaccggca acgtcctgcg cgattatctg accgacttgt
     5461 tcccgatttt ggaactgggt accagcgcga aaatgctgtc catcgttcca ttgatgaacg
     5521 gcggcggtat gtttgaaacc ggcgcgggcg gttctgcacc gaaacacgtc caacagttcc
     5581 tcgaagaaaa ccacctgcgt tgggactcat tgggcgaatt cctcgcgctt gccgtatcgt
     5641 ttgaacatct ggcgcaaaaa accggcaacg ccaaagccca agtcctcgcc gacactttgg
     5701 atgccgccac cgaaaaactg ctgttgaacg acaaatcgcc caaacgcaaa gcgggcgaac
     5761 tcgacaaccg cggcagccat ttctacctca ccctctactg ggcgcaagaa ttggcggcgc
     5821 aagacaaaga tgccgaactg aaagccgcat ttgctccatt ggcagccgct ttgactgccg
     5881 acgaagccaa aatcgttgaa gaactctctg ccgtacaagg caaagcggtc gacatcggcg
     5941 gctactacgc agccaatcct gaaaaagccg cacaagcgat gcgtccgagc gcaaccttta
     6001 atcaagtact aaacgcctta taagcacaaa ggcaaaaatg ccgtctgaac attcgtcgtt
     6061 cagacggcat tagttatccc tatctgcctg attatgatta aacgccgcac ccattggata
     6121 tatagtggat taacaaaaac cagtacggcg ttgccccgcc ttagctcaaa gagaacgatt
     6181 ctctaaggtg ctgaagcacc aagcgaaccg gttccgtact atttgtactg tctgcggctt
     6241 cgtcgccttg tcctgatttt tgttaatcca ctataaatca tataatcttt agttttttac
     6301 cctacggaaa gacaataata tgaaaactgt tccaaccatc ctgaccggca tccttttggc
     6361 aaccgccctt cccgcatccg cacacggtat gcataagagc aaacccctgg ctatggacga
     6421 gctgccgccg atttgccaac aatatttcaa acgcgccgaa acctgttaca acaaagccgg
     6481 aaacaaagca gattttgcac gcaacaacac caaattccta ttccaagccc tacctgccgc
     6541 cgatttggga cagcgtaaac aaatgtgcca aatcgctatg gattcgtttg cagaaaaaac
     6601 ccgcaatctg aattgcgaat aaagccgcat cagcaatagc aatgccgtct gaaacaaagg
     6661 ttcagacggc attctcacat caaaacgtac cggctgataa aacccgcctt ccccgcatcc
     6721 ctgacccgaa caggattaaa cggagtaacg gtttcggcca ttatggcaag tccccatccc
     6781 aaaaatgacc atataaatta ataccataaa acattgataa aaagcgtctg ccggttgcgg
     6841 tttgttgcag ggcgttacgg aaaatcatct taaaccgatg atttttatat agtaattgac
     6901 acaacctgaa caaaaaagaa ccgccctgaa tcagggcggt tttgctttgc ggcggaaacg
     6961 gcaggattcg actaaatttt attttattga tttaaataca tttatttctt ataaaaattt
     7021 aatttaccat aaaaacagcc atatacaaaa atcttggagt aactactgca ttacactatt
     7081 agaaggatgc ggggtcatgt aatatttaat taaattaata tataaattaa atatttgaat
     7141 tatatttata aaaaaaggtg caaataaggt acaaataaaa ttagtttttc ccaatgggtt
     7201 aaagaacaaa tccgaaaaga gttcttaaaa tagcccgacc gccgccaagc cgcaaacttg
     7261 gcggcttctt tatgccgtcc gaagggcttt cggacggcat ttttctttga ttttgttggg
     7321 ggaatggttg actttgaacg tgtccacgtt tataattcaa ttcatcggcg catcgggtgc
     7381 cgatacggga agagccccac cgaagcgggg cttgtacagg gagtaggaaa atgaagctca
     7441 tccttcaaat tctcatcctg gttttaatct tgatgctggc aggcgatacg cctattagcg
     7501 gataaaaccg gctggggcgg tagccgccgc cccagtccct atcttacaaa aggcagttca
     7561 aaaaatcaag gagtttgcct atggttaatg aaaaactggc agaaaaccga aagcgttacg
     7621 agcaaaaacg agtcattaaa aaagtctcgt ttaatgcgga aacagaaaaa gagcttttgg
     7681 aatatgccca aaatctcgat ttttcccaat gggttaaatc aatcataaaa gaaaaaatca
     7741 aaaagtaatt gactttgaac gtgtccacgt ttattattct tacaacggca cagccgttaa
     7801 tggaaaaccg aaccaagcgg gaacttggtt cggttaagtg aaaagcagtt ctaggaaaac
     7861 tcttttcgcg tcttttaaat aacctctaaa agaaagacta ttctatcatg aatgctgtcc
     7921 aagttttaaa ctttcaacaa aactccgtcc gtactgttgc cgataacaaa ggcgagttgt
     7981 ggtttttggc aaacgatgtt tgcgagattt taggctacac caaccctcgc cgaacggttg
     8041 acctgcactg caaaagcagg ggcgtaacaa aacggtacac ccccaccgcc agcggcgaac
     8101 aggagatgac ctatattaac gagccgaatc tctaccgctt gattatcaaa tcccgcaagc
     8161 ctgccgcaga agcgtttgaa gaatgggtaa tggaaaccgt cctgcccgcc atccgcaaaa
     8221 caggcggctg ccaagtcgga ccaaaaacca ccgccgacga ccgtaccgga ttgcgccggg
     8281 ccgttgccgc gcttgccggg cgcaaaggca tagactactc ctccgcgtac agtatggtac
     8341 atcaacgctt caacgtcgaa tccatcgaag acctccctgc cgggaagctg cccgaagccg
     8401 tcgcctacgt ccacgcattg acactgcaca cgggcttgac gggcgaagtc cttgaccgcg
     8461 aaccgttacc cgcgccgcaa cccgccctgc ccataagcgg caacgctttg gccgacattg
     8521 ccgctatggt ttattacggc acacggatga ttgaatcggg caaagacgtt tccgcaccgc
     8581 tgaagcagct cggctgcagg caggcggtta cgatgtggac ggtttggcac gaaacccgtt
     8641 caatcctgaa aagatccgcc gcggccctcg aagtgttgcg gggatatgcg aacaaagacg
     8701 cctccgaccg catagccgca tgtcttgaag gcatttacag caaggctacg gtaaggtaaa
     8761 cggaaaatgc cgcccgaatc ttcggacggc atttttcacg cggcatagcg tttcaacatt
     8821 tcccgataat ccgccggttc ccgtccggcg aactcaaagg ccttcaaatc ggcgttatct
     8881 gatgccttgc ggctctcgtt cagccaatag gcgatctgtt tcctgcaaaa ctcttcagca
     8941 ctcatcgtca aatccaaaaa tgcctgttca ggtgcggcgc atcctcacgc gtgatacggc
     9001 gcaaatcact gtcggccggc aggccgaaat acagctcgaa acgcctcctt gccgattccg
     9061 aacgctgcgg gtcgaaagtc tccgcatccg ggatactgaa agcctccgcc aaagcccaat
     9121 aaaccaaatt ccgatgatgt atggaattga tttccggctc gtccccgtcc cccgccatat
     9181 cccgcggcag gcaatacccc ccggcaaaca acctgccgtc acggtccggc gcaggcgcaa
     9241 gcgtcagctt cccgtcgccc tgcaccgcat aaaccggcag ccccgtttgc gccccgtcgc
     9301 gccaacccgg ggcgcaaaca tccgccgcct ccggcgaaac caacttcagg cacaccggct
     9361 tgccgtcgga cacaaaacgg atactgtcga tttcatacgc ataacccgcc gcaaaccccc
     9421 ctgccgcata agcataaagc ggccgccctt tttccacatc cgttacaaac gcatcgtccg
     9481 aatgcagcag ccggccgcgc aggcacgcct cgtgaaccgc atcgttcaac catcccgaca
     9541 cctcttcgtc gctccaaaaa tacggctcgg ccttatcatt ggcaagcacg cggaaacgcg
     9601 caatcaaagc cttcaaattc attgcgcgat accgaactga cgaaccatcc gaaccacgtc
     9661 cccgcgcaaa ttctccgccg atttgcgctt atccaaaccc tggccgtaat tcgccttcgc
     9721 atacagttca agctcgtcct tgcccatccg gttgatttgc aggatcacat cctgcaccgc
     9781 gtcaaacgcc gccgtttccc gttcgagttc cgaagcgccc gccttttcca aagcctccgt
     9841 atcatccgcc ttttccacgc gtttgcccgg gacggcggca aacacatcgc cgtggcgcaa
     9901 caactttgcc gcagccccgg ccgacacctc gcgcacctgg ccgtcttcga aaaccaagcc
     9961 cgtatgatag atacggtcgc gccacacggc acgcccgccg atatacttga taaaaacctt
    10021 atccatccaa ccccccgccg ccgtccggac ggcattgtca aatacagcaa cgccgcaaag
    10081 gcaaatcccc gggcggcgtt tcattccgct tacaactggc cgaacttctc gccgtaaatc
    10141 aaaatatcga tatcggcggc cttcttattt tccgcccctt taagcgtaac cgtcagcaaa
    10201 gcctgcttgg gcagcgtaac cagtttggca gactggcagc ggatacgccc ggcagacgca
    10261 aagtccgcgc ccgaagcaaa ataagccgca tcctgaggca ctttcgcatc atccgcaccg
    10321 tccgcatacg caaagccgac atcggcagtg ataccggaag acatcgccgt cttcacaata
    10381 atctgcgcgt catccaaacg gaaaccctgt tccaagacac ccagcgccaa cacatcgccc
    10441 gccttaggcg gctcagaagc atccgccccc aagatcgcac cgtcctgctt cgcaacgata
    10501 cggtaatgct ccgccgccag attgccgtaa ggcacgccgc cgaaacggtt gccgccattg
    10561 tttttcacat aaatctcagc cataaaaaac ctcaacccaa caaataggaa aagccgccct
    10621 ttcaagcggc attcgaatca gcggataccg ccgtgcaaag gaaccaccgt atccacaacc
    10681 gtgacaccgt agtcggtaat ttccttcgcg cccccgccga catccacggc aaagcgcgtt
    10741 ttcgccacac cgcgtatcgt accgacgagc gtttccacac ggttgccgtg gtcaagctcc
    10801 ttctcagacc aaaagaaagg cataccgccg tgtttgccgg tattcgcaaa cgcctccaac
    10861 accgcctggc cgcccaaaat aaccgaacgg tcgaccgcaa acttgtccgc aaacgaagcc
    10921 gggattttca agcccgattc cgcctcgctg aacttatcgg cgcaatactt catctcatcg
    10981 cccgcataga aacggatggg gcgcggcatt ttcaccagga taaagccgtt ccacaaaccc
    11041 gcatcgccca aaaacagcgg attttgattt gcctgggagg cgcgcgcgat tgccgaagcc
    11101 tgaagctggc ggaatttcgg atcggcggca aaacggttgt actgcgccgg ggaaagcagc
    11161 cacacgcgca aaggcgaatc acccgccgcc ttgtcgccct caaacttcac aatcggcggc
    11221 ggcagcggaa tctggtcgag cacggtacgc atactgtcca ccgtatccat cgtaaacaaa
    11281 tcggtagagg caatcttcaa ctcgccgccg ttgtccccaa cgcccgttac cgcatcgccc
    11341 gaagccgtaa aatggcggtt tttggacggg gctttgaccg gattgaccgc ataatcattg
    11401 aatttcgggt cgcccgccaa aggcacgccc cattcgatat tgtcatgaaa accgcgcgcg
    11461 cccgccatat gcaccaacag cgtctggtcg gcataacggt ccatcaggct ttgcgccgcc
    11521 ggacgaatca gcgcgcgata atcggcaggg ctgcgctgat tcgtcatcgt gccgccgcca
    11581 acggggaaac gcgcctgatt cacacgcaaa cccgcctccg acaggctcat ccccacgccc
    11641 ctgccttccg ccgtgttgtc gcccataatc ggcagggcgg aaacagggtt gaccaaattg
    11701 aaacggattt cgtcacccat gccgcgcgtc aaatcctgac agcgcacgac cggcatatgc
    11761 tgggtcgtct gtttgcgcaa agtcgcctcc gcacccgcgg taccggcagg catcttgccc
    11821 gccaaacggt tcagcgtact gttgcgctgc atatgcatcg caaacagccc cgccgcctgc
    11881 ttcatcatcg cctgcgggtc gccgtaggcc gtattcgtct tttgtgccat aaagagcctc
    11941 cctcaaaaac tcaagaattt aaaaacttct caacctgctc ttccgtcatg gaagccagtt
    12001 tttccgccag cgcgttgccg ctcaaataat ccggcgggcc gtccgaagcc gtatggtcgc
    12061 gccccgccgg aatatccgac agcgtattgg gcgtattctt cccggcaggc ggcttttcgg
    12121 cgggcttttc cggttcggcg gcggattttg ccgccttaaa catatcgaac gccccgatga
    12181 cctcggcggc agtcccgtcc ttaagcgcgt cattaaacgc cttgcggaca agcgggttct
    12241 gcccggccag ccactcctcc agctcgcggc tttccacaat cgaatccgca tcggggtgcg
    12301 ccgtatagat ttcgcggtaa tgcgcctttg ccgcctcctg ccgcttcatg tccgcctcat
    12361 aacccgccag cttctcctga atcagctttt ccacgccctt tttcacatcc tcgtcggaaa
    12421 aatccccgaa caacgaatta tcggaaccgt cagccgtttc cactccgggt ttttccgccc
    12481 ccttctcaag ctcctcgatg cgctccctca aggcttgggc ttcggctttc gccgactttt
    12541 ccgcctcgcg cgcctctacc agcttttcat aagggatagt gtgtttgccg tccttggcac
    12601 ggatgacctt gtccgcacgc cgtccgccgc accgataccc gcgccgacca ccgcgcccgt
    12661 actgccgacc accagcgatt ccgcccactt cttaccctcc aaaccgtctt ggatatcggg
    12721 gaaaaccaac tggttcaagc ccaaacgccc cgacacgtcc agcggcgtca ggcggctgaa
    12781 gcccttggcc aacacctcgc ccgccttatc cccgaaagca tccgccaaca tattgcgcaa
    12841 cgccgcttcc gcatcccacg ggtcatcgtc gtcactgccc aacatcgaag ccaccgcaag
    12901 cagcgtcgac acaaacggca gccccaagat gcccgaagcc atcgcatgcg agaccaacag
    12961 ccccgccagc gtcttacgcg cctccttatc ccccttaaac gcaaggtacg cattgcgccc
    13021 cagcgcatac aggatattct gcgaatactg cttgaacaga aagaccacct tcgccgcatt
    13081 gcccatcata aagcgcggac ggttttgcgc cgcatagtca aaatgccagt cgtacgtcgc
    13141 ctttttcgcc tgttcgaaag ccgcctcgct gtccgccccc gcccgtttcg ccaaacggta
    13201 ggccgcgaca aacgtaacct ggcggttaaa cttctccgca tgatggaaca gccaggccgc
    13261 cttatccatc accttctgcc aagccgaccc cgccaatccc gggtctccgt tcgccacccc
    13321 cgccaaatca tgcgcctgcg acaaatcgat cacaccgtaa tccgccgcct ttcggaacgc
    13381 cgccttctca tcctcgttca gactgtcctc aatcgtattg aacttctccc cgaacctcag
    13441 cccgatctgt tttgaagccc gcagcaattc ccgcgccgca ccggcataac cccacttcgc
    13501 cgccatcacc ggataagcca ccattgccgt ctgcgacagg ttcacaaccg cagaagcggg
    13561 cgacatcccc atcatccaca aaaagccgaa gcccgtcaaa gcctgcgcca gcttggaagg
    13621 attcggattc atcaccgcct cgtggcgttt cctcatctca tccgccacac gctgcaactg
    13681 acgctggtcg aaaccctcct catatttgcg cccgtccaca aaatcctgca tcacatccaa
    13741 ctgttccgcc atacggtccg catagcgcaa cttcgccaga taattcgcac cgctgcccat
    13801 attctgcgca tatgcgcgcc tcgcatcatc gctgaagccc ggcacgccct tgcggtggat
    13861 gccgtgtttc gcccaagacg tatcgggcag ggagttcaaa taaagctgcg tcagcgtatc
    13921 gttcaatcgg gcccgttgcg ccggatccaa atccatcctg ccgacagcct cgcccagctc
    13981 cttcataaat ccgctgccga ccgcatcgcg gcttcggata taatcccggg acttcatcac
    14041 gggcgaaacc ttaaaccccg ccccgaaatc agccttcagc gcatcgcgca gcttctccgc
    14101 ctcgctcaaa gtttccgcgc gggacacatt cgcactattc ccgtccgcat ccttgaccac
    14161 caccagataa tcgccgaaac gcgccaaagg gaaatacaca ccgcccagct cgttgtcaaa
    14221 acgctccttc aggcggcgca ccgtttccgc atcctgcccc gcacgcgcca accgttcggc
    14281 aagcgcatcg cgcacctgcc cgaaatgcgc cctataatca tcccgcgcct tgcggtacac
    14341 cctctgcgcc gcatcaggca gcccggcaaa acggcggttc agcaaagcaa gcccctccaa
    14401 agccttttgc gccccaacct catccttttc caaacgcttc acatccgcgc gcgcctgctc
    14461 cgacatccgt accgcatccg ttttagcccg gctttccgcc gccgcatccg ccccggcgcg
    14521 cctcaacgcc cgcttcgcct ccgaatccgc ataaaacaga cggcgcaggc gcatatccgc
    14581 ctcatccgcc aaaatttccc gaccgtgttt ttcctgcgcc ttctccagcg cataagccgc
    14641 cttatccgcc gcccgttgcg ctttccgtac cgcggcatcc gcacgcgcaa tacgcgcacc
    14701 ggcggaagca acggccgcct ccgccttcgc gattttaccg tccgcaatat ccaaagccgt
    14761 ccggatgccg tccaaacgcc cccgggcatc ctttcgcatc agcgggtcgg catccacctt
    14821 cgccagcgtc gcatcgtgca tcagatccgc aagcgctttc gcatcctcct ccttcaacct
    14881 gccccattcc ctgacaacct tgtccgcctc cgtaaccgcc ttgttcgcat ccgcgccgaa
    14941 gagttccgac aaacgcccgt attccttcag gccgtccaaa acgccgccgt aaatgtcctc
    15001 gatctgccgc ctgcccaaaa actggagctg aatccccttg gaagcatccc agcggtcttt
    15061 cagcgcgttt ctcaaatcgg attttttacc cgttatatga ccgattgcgg ccccgatgtt
    15121 cgccgcacgg gaaaaacgca ccgcctccgt gtcaaaatcg gcaatgccgc ccttgcccgc
    15181 atctttgtct tgcaaaatct gcaacaggcg tttatcatca atgcccgata aaggcgattt
    15241 gctttcagag gctcccgtag ttgatacgcg gtcaagtaat gaaagcaagt tgcctttttc
    15301 tattttcgtc agcttatggt cgtaatagcg gttaccgttc ctgctaaccg cgacggcagc
    15361 ccttaccgtg taatccgcac cgccgacatt cagtcccgaa acataatatt cataaccctg
    15421 aatatcgccg ttcttagcca aatcctcgtt cggcagcgta tcgatataca ccgcattctc
    15481 aataatctgc ggaattgccg cgatactctg caaatgttcc gcgtccttat agtcgtgacg
    15541 caatatttca gtgatgcccg aacgtcccaa actgatttca cgccccgtgt ccttattcac
    15601 ataaggcccg cgcaaagatt tgccgtattc cagcgcattg cgcttatatt cgcgcaaatt
    15661 gccgccttcc ggaacctccc tgcccgagat acggatggtt tccgcacggc gcagcttctc
    15721 aaggctttcc gacttggaac gttccgccgc accgtcttcg gcccgcgcga acatcactgc
    15781 acgatgattg ccgccccaag gcgcatcccg cgccccttcc acattcccgt gcaaatccgc
    15841 cagcatcgca aacacatccg cgtccgacat cgcaccgccg gccttgcccg ccaggcgttg
    15901 cagcacacgg cgcaacaggg cgccgatacg cgccaacacg cccgcaatgc cgcgtttcaa
    15961 accgttgccg accttcacgc cgtaacggtt ttcaatgccc gcccaaccgc cggtacgctg
    16021 cgccgcataa agctccgcga ccgcctcttc caccgcggcg gggcgcaccg aggccgccgc
    16081 atcgcccgtg ccttcgcgcc cttcctgcac cgcgtccgca atgcgccgta tcaggccgtt
    16141 gccgtccgca cgttccaatt cttcacggta cttggcgaaa ccatccgccg caaagcccct
    16201 gtgccccagc tcgtgccacg ccgcccatac cgcacgttcg ggcgtaaggt tttccgccac
    16261 cagcgtgatt ttgcccgtcc tgccgtcaaa ccagccctcc acacgctgcg acaacagcat
    16321 ctgcgccttg tccggcgccg tttcggacac ggcggcgaca tccacccgtt ccgccagtcc
    16381 gcctatgctt tcggcaaggc gttttttgat attttcggcg tgctcgcgca atgccttaaa
    16441 accttgcggc gcctgacgcc ctttcacggt ataaacaggc cctccctgca acacacgcgc
    16501 cttgcgccgt gcctcttccg gattgtccaa agccgttttc gcaaacgttt caggttcggc
    16561 agccttatcc gaagacggct tttcggttgc cgcgccttgt gcggcggccg acttgccctt
    16621 tccgccatcg aagtgggatt ttcccgcttc gatatttcgc accttatccg aagacggctt
    16681 ttcagttgcc gcattttttg cagcagccgc cttgcccgca acaggcgcgt cttttgcccg
    16741 ggcaggctgc gccctgcccg ccggtttccc ggcagaagcg gcatcgcgcg gccgcgccat
    16801 caccttggcc ggcgcccttg cttcctgcgt cggacgataa tcccgacccg gctcggacgg
    16861 cacggcttcc gacaaaccgc ccgcccgcaa ggtttccgcc tcccgcccct tcaaataacg
    16921 gcggccgtcg gagacaaggc cgtcagagtt ggaaggaccg gccatatcgg caaactcctc
    16981 cggcatcccg cctttgcccg gcctgcccgg cacacccgca agcgcatcaa tatgcctgcg
    17041 gtccgtatgc tcgaaacggt attcgccgcc ccccaaaggc acacccgcct tttccggcct
    17101 gcgcctgtcc gccgccaaac ggcgcgtgaa atccgccgcc gcatccgcca tccgtttctt
    17161 aggcgatacc ctgcccgccg attccgcaac ctgcggattc aataccggca acaccgtatc
    17221 ggggcgtttg cccgcaacag gcgcacgttt cccgccgtcc gtcccggcaa ggttttcaga
    17281 cgcggacagg ccgggcgcgg caccttccgc gcgcgcccga acctgtaagt tttgccgacg
    17341 ggccggcgca ttgccggacg caacggcgga ccctggcaaa ccctgcaagg cagccgcatt
    17401 tgcatccgcc cccgcatcag gggtcaaacc cctgccgcct tgggtcaaac cctgccgcac
    17461 atcctgccgc acatcctgcg ccaaaccctt atcttccgtg ggtaaagccc gggtcaaacc
    17521 cctgccgccg ccgacaacgg cctcagacgc gccgtcatcc ggcaacgccg tccgaacccc
    17581 ctgcaaacgc tccccctcgc ggctcaacag cggcacgctc caaccgttgt tgcggcggta
    17641 accgtaaacc gcacggttaa gcctgtccaa cacatccgcc gattccggat gttgcgcacg
    17701 gtaagcgcgt acagccccgg ccgattccgc caaaccctca ggataatgtc cgcgcatatc
    17761 cgccataccc gcctcccggc gcataatccc gtccgccgta tagtccgcca ccgtctgcgc
    17821 atgacggcgc acccattcag gcagcgcgtc aaaccgcctg cgcgattcct tatccaacgc
    17881 atcggcaggg acggaaaacc cttttttagg aacaggcacg gcatccgcca tacgcacggg
    17941 cggcggcagg taaccctgtt tggcttcgga agccgcctga taatccgcct caatccgcgc
    18001 gtgatacgcc tgctcgattt ttcggcgcac aggcgcggcg atttgcccga aatccgcaac
    18061 gcccttaata tcaaggcccg cctccttcgc caaagccttg cgcccccggg tatccaagcc
    18121 gtcgaaatat tggcccgcag gcagcggcga gacccgcgca atcccgcccg aagcggcggc
    18181 ggcagaaccg cccggcgcaa ccgaagccgc accgcgcggc gcaccgccgg ggcgcacatt
    18241 gccgtaagcg gacggaacac cgcccctgtc cgcatccgcc gaccgtccgg acggcacatc
    18301 atccgcaacc gaccgggctt ccatatcgtc agacaccgca gacggcacag ccgcgcccga
    18361 atccgccgcc aaagcagcgg ccgcagaaac cgcaccgtca tccggattaa tccccatcgc
    18421 ctccgacggc ttggcccggt attcggccgc catgtccgca tactgacgcg ccaaaccgtc
    18481 cggcatcacg cccgtatcca aaaattccgc cccgtcctcc gacgggatac ccgaaacatc
    18541 cggctgtttt tccgtcccgc gcgccaattc ctccgcgcgg ggcgtattgc cgtcggcagg
    18601 gccgctgaaa aaccgccccg cctcctcctg cttcgtacgg tccgcaaact gccgcaaagc
    18661 cggatgcgac tgatcgtgat gcgccgtatc aaaacccgcg ccgccctgtt ccgcgccgcc
    18721 gtccgccgcc gcacgcaacg cctgcgcccc ttccggggcc ggcctgcccg ccttcctgtt
    18781 gcccccgata cccccctccg cacccgcctt ccgtccggcc ggcggcctga gcgggtcagt
    18841 taccgccgca taagccttcg ttccggcaaa ccccaccgca gaatgcccca aagaagagaa
    18901 agcctccaaa gccgccccct tctcgtccca ttccccgttt gccagccccg tacccaaata
    18961 ctcgcccgca aactcaccgg ccgattccgt cgcataagcc gccgcgtgcc gtgccgcacc
    19021 gcccaaaccg cccttggcag actccttcgc cagcgccgca aattcaggcg ttcccttagc
    19081 cgccgcaacc gccgccgcaa cacccatttt ctccaacgcg gctttcccgg cggccttttt
    19141 gcccatattc aaaatactgc cgccaagttt catcgccgcc atatccgccg cgccgaccac
    19201 cgcgcccttg accgccgcat ttttcaacgc acccggcgca ccgcggccga taaacgccat
    19261 caccgcatcc ttgtccgcag ggtcgacgcc cgccgcctct gccgccctgt ccagctgccc
    19321 gccgtattcc atcagcgtat tgcccaaggt cgcgcccgca taggcgccga cagccgcgtt
    19381 accgcccgcc aaagcgccca atatgccgcc ggcggttgtc gtagcaatag gaacaagcgc
    19441 attgggcgcc tgctccagca ccgcacccgc catctcgccc acatcttcca aggcgccgtc
    19501 caaacccttc tcgcgccagt ccttcttaag ctccccccac acattcgaca ggccgccgcc
    19561 gacacccccg cccttttccc aagcctcgcc cagctccctg cgcgcttggg atttgcgccc
    19621 ctcctgaatc ttccgataat ccatatcctc ggccttgagc cggcccagtt cctctttgtc
    19681 gcccgtaagg gcattccacc ccatacgcgc cgaacgcata gaatttttaa acccgcgcgt
    19741 caacgccgta tcgtcggcgg ccgcccgcgc atccttttgc gtaaagccgc ggtactcctt
    19801 caaacccccg acaagccgcc cgtgttccaa cggatcgtat ctgaccaaat cactcattgc
    19861 tcaaccctct tctcaccctt ctcattctca taaatgattt taccgccggc cgttttctga
    19921 ccgttcggct taaagcccaa cgccgcataa cgcttctcgc gctctcccgc aaactccaca
    19981 tccaaaggct tgcccgtccg caaatcgaca atcccctcgc gcttcgccgt caagcccgta
    20041 tccgggtcgg caacctcata actgattgtc ttgaacacat tcgggtcaaa gcccttctcc
    20101 ccttgccgct caaacttccc gttaagcgca tcaatctgcc gcgcaatctc cccgcgctgc
    20161 ccgtccgact tcgcaaaacc ataagcccgc gtcaaatcgg cgatgcgctt cctatcctca
    20221 agctccagcg catccgcctc acgcttcatc tcaaacccct gccgcttcat ccccagctcg
    20281 gcctgctgca catcaaacgc cctgtccgca ttcctctgcg ccatctcgcg gttcaacgca
    20341 ccctcgttcg ccttaaccgc cgcatcaaac cgccccttct gcaacaccgc ctcacggttc
    20401 gccgcattct ccgccgcagc cgccgccagc ctttccctgc ccaattccct gaccgccgca
    20461 tttttattat gttccgccac aatcccgcgc gccgcattaa gctgcgccgc cgtcaactga
    20521 ccgttcaaag cccccgcata aggcgtagtc gccctcctgt aaagctcccg ctcctcaggc
    20581 gacagcgcat ccggcccggc agacaccaaa ggacgctgcg cgtcaaaatc acgcaccggt
    20641 ttgccgtccc gatacacatt cccgtcaaat cccaaaggcg gcggcgtccg tccgcccgca
    20701 tccgtcgggc gcgtatcgcg ccgcgcgaaa tccgccccgg cccgcgcatc gggggaaagc
    20761 gccttatcgc cgcccgaaaa ccccaaaccg gcatccgcac cccgttcgaa agcacccgcc
    20821 cggcgcaacc gccgcgcccc atcctcaata ttgttacggc tcaaatccat cacattccga
    20881 taacgcgccg gcccggcctc ataattcgca acggtacgcc gcaccgcatc ggcacggtgc
    20941 gcctgtgccg ccgcccgctc ccgctcctcc gcccccttcc gggccttctc ttttttctcc
    21001 ttttccttca tctcccaaaa atccccggca gacggctcaa acaaacccat cgcaaaccct
    21061 ccgaacaaat aaagcccaaa ataagcaaaa acccaaaccc aatcaaacgc ctaaagttaa
    21121 gaacaaacaa aaatcgcaac cacaaaccaa ttaataaaca acaagttaca caaaaaaaca
    21181 caccgacggg ccatttccgg gggcaaataa aaaccgcctt cgtgcaaggc ggttgcaaaa
    21241 aagcctttca tttaacgaaa ggctttttgc cgcccgcgta ccgcgttcag aggttgtccg
    21301 acaccgtcgg cgtttccatg cctgtttggg cgtgattcgc actctttggc ggcacggccg
    21361 tcatcgggcc ggtatccgcg ccgtaggctt cagggatgcc ttcgggcggt atttgcgttt
    21421 caggcggcat ggcggggacg gggaagccgg gatcgtcgcc ccgcgccggc ctgacatatc
    21481 ccgcgccctg catgaccgcg tcggcaacgg gggcgatttg cggcatggcg gctatctgcc
    21541 cgcccgcctg catggccgca taagccgcct gcacgccgat ttgtaccgcc cgcgcttcga
    21601 tttccttgat ttcgctcgcg gtacgttgtt ccttgagcgc caattcccgc cgtttgatgt
    21661 cgttgccgga ttttgccaat gcgtcctgca ccgcctgcgc gatacgcgcc tcgatttgtt
    21721 cgggcgtttc ctgcactcgg acttctttga ttttttcaat cactttgtct ttaaacggga
    21781 tgtccatcag ggacaccata aacggcagca ccgccgcctg atattcgggc ggcagggatt
    21841 tgaccgcctc gctcatcgca cccagctgct ggctgcggta ggagttggtg ctgggcacgt
    21901 cttccaaagc cactttcagc cgtatgtttt gcaggtcgtt ggacaaataa gccttgccgg
    21961 ttacggggtc ggtttcaggc ctgttgatga cgaccgtccg cccttgcgtg acggcgtccc
    22021 cttctatgac gacggtttgc tcgtccgagc ccaaatcctc gataatcatc gcaagcagca
    22081 actcgccgac caatgagcgg ctctggcgga agttgtccat aaccagcccc agcgactggt
    22141 tggactgctc gacttggatg ctttcctgcc tgccgctggt ggcgttgccc cggttgccca
    22201 taaatgacgg ggtaatcccg ctgatttgcc gtattgtcgc gcggctgtct tgaagcatct
    22261 gccaatgctg tgccgacaat tcgaaatcgc ggctgacgtc gaaacgcgcg cccggctggg
    22321 cggcctctat tttgttcagc acgatgtccg cgtccacccg tgcgatattg cggcggaact
    22381 gttcgtccga catatcgact atgcctttgg tgcgtaccgt gcgtattgcc gacaaacccc
    22441 atcgtaattt gctgttggtg ctgttgaggt tgtcctgcgc gtatttcata ttgcggacat
    22501 atccgtaggg gatgccggtg ttgtcctcgc ggaatccgaa aaacgggacg taagggaact
    22561 tttgatgcgg atacggggtc ggctcgtcgc ggacgaccaa atcccccacg acaaaggcgc
    22621 ggcgcatacg cgggacggaa gcggcaaaca atagcgcgcc gtttgccgcc atctcccgat
    22681 ggtttggatt tgcgccgtca aactccaccg tgcgccctgt ttttttgtcg cgcaggcagt
    22741 ctgccgttac ccaccgcctg taccatactt ccgccaccgc cagttcgcgc gtggtttcat
    22801 tgaaccaaaa ctcttcgctg acggtgttgc gccccgaaat accccaagcg tccgccagtc
    22861 cggtattgcc gccgccgtca agcatttccc cgctgatgcg ccaatccgaa ccgccgcgcc
    22921 ccatcgcttt gaaatgtccg gcatattcag ggaagaattg cgccaggcgt tcaggcagca
    22981 gccagcggcg gcgtatcagc cagcgggcat cggacaggtc gtatttgtaa gacttcatat
    23041 cccaatggat ggcgttgcgg tggatgacgc cgcactcata aggaaactcg aaagggttgg
    23101 ggttgcgtgt aacctccacc cagccgatgc cgcaggctat ctgccccctg aacgcgtccg
    23161 aacaagcctt gtcggcacgg ctttgccgtt ccgcgcggtt gagtttgaag ttcaatgcgt
    23221 ccgccacgtc ccgcccgccg gtttcgccgt ccgccgtcac gcgccagtcc gtccgtatcg
    23281 tcgcctcata tccctgtacc gacagcaggg tcggggtaat gaggttttca acggcgggcg
    23341 gcaggcccaa ctcgcgctgt ttctgtaaaa gctcgttgtc aagctgcctg ccgtcggcgt
    23401 agtccatttc cttgtcggca acggcacgcc atgcaggttg gttcatcatt tcaccgacaa
    23461 acagccggta ttccccgata gtcagcggtt cgccgttttt atcgggcaat acgcctgttt
    23521 ccggtacgtc cgtccccatc aataaagcct ccaatccgat acgggcgttt ccgtgtatcc
    23581 cgtattgctg cctgtatcca ttaaatcaac cgcctgcgcc aggtagcgga acatatccgc
    23641 gccgtgcgaa tattcgtcgt gcagcggccc cattgccacg cctgttttcg catgtatttg
    23701 gcggccgtac cgtttcaggc attccaaaag ccgcgctgtt ttgtctttat cgaagtacac
    23761 tttgggaaac agcatccgcg ccgccctgat gccttcttcg ataccggcgg cgttttgcac
    23821 gaaaaccgac ttgcgcccca gtccggtcaa aatctccatc gtacttttgc ctgtttggaa
    23881 gttgcgcgtc ctgccgtcgt gcggcaggaa gtccgtcccc caccggtagg gcagcttttc
    23941 caattcggca acataccagt ccaacgtccg gtgcgtgtct tcgatgtagc ctatgatgcg
    24001 cacgctcgtc aaatcgcgct gcaccagccc gatggtcatg gcatcgttcc agcccaaatc
    24061 ccaaaccgta tgcacgggca aagaagaatc ataagggacg agcgtaacgc gtccggaatg
    24121 aaaagcgtcc tgtatttcat gccggtaaac cgccccctcc gataccatgc gcggcctgcc
    24181 ttcccaaata ttcccgtagt cctctttatt catcgaacgc tgtgctttga gccgctcccg
    24241 gttcaatgct tcgggaaacc acggattgtc gcgccagttg atttcgcaaa gccaagtgtc
    24301 ttcggacggc atagcgataa aacgccggta ggtttcgtcc gtctccatat cgggattgag
    24361 ggtaatccaa atttccgaac cttctttgcg tatggtcggc gtgagcacgt cccagctttt
    24421 tttactgacg ccgtgccctt cctcaaccca tacgatgtcg ataccttcaa acgatttgat
    24481 gctgtccacg gtatgcgact gcaggtccga aaatacaaac agcgtgccgt ttgcgcctcg
    24541 tatctcgaag tcggttattt cgtagaaatg ccccaaaccc aactgcgcca ctttgtcttt
    24601 caacaggcgg tgtacggaat cgcgcatcga tttttgaatt tcgcgcgcgc ataaaatacg
    24661 caaagggcgt tgcgccccca gcgccagcag tgcggatgcc gcgccgtgcg atttgccgcc
    24721 gccgcgcccg ccgtacataa ccttgtaccg gcaaggcttg aacagcccgt ccagttttgc
    24781 gggcaatttc aaatcaaccg tctttcccgt catcttcagg ccctacaaat tcgatacgga
    24841 tgcccgccgt cttggcggat tccgaaatct tcgattcggc ggtatccata ccgtatgatt
    24901 tacgctcgat ttccaccaaa accttaaacc ccgccgccac cgtttggaac atacgcgcct
    24961 ttgtgaatgt gtcctccggg cttccgtaca attccgcgac aatctgctcc gcgcttttgg
    25021 acaggttttc cgccaaattc cgatgcctgc gctgtacggc ggcaacccgt ttcgcttccg
    25081 cgtccgttac ttatttaagg ttgaaatccg cataatccgg cgcattgccg tttttgtccg
    25141 cggacagcac catcgcccgc gcctttgcct gtacctcgtc attcaaatcg cgcgtccaca
    25201 cgcgttcttt ggcaaccctg ccgacgtgtt gcgccgaaac attgtatttg cgcccgattt
    25261 cggcaatgct caaaaccccg cgccggtaat ccagctcgac tttgcgccaa tcctcatctg
    25321 acaatctcgg cattaaaaat ccaacctccc gtttttggcg gaacattcca gataattccg
    25381 caacactgaa aactcgcgcc ccatctgaat actggaaaac tgctgtccga accccgacat
    25441 caaagccccc aacgagcgca tttcctgcgg gttgaggtgc aataccgtat cgcctatttc
    25501 caatacgccc atccccgaat ccaataccga tacgctgacg gggcgcgtct cgccaaaccc
    25561 tatggcgggc ttgaaaatcc cccttgtcac gcggtagatt ttgccgtgct cgaccagcag
    25621 cttggtaaat ttagtgattt ccgacatttt caggccggtc gtttccgcca aagcctcgcg
    25681 gctgaccacc tgctccgcgt tgtgcaaatc cacaaccccc tggtacacct tgtcaatatc
    25741 cgacaatccc gacatcaaat caaactccta aacatcccaa acgattccaa actctaccgc
    25801 cgcccgcgat tgcaggcggt tacactacaa agccaaaatc tttagatgct gcctctctcg
    25861 ctttcactgc atcttcaata tgttcaaata aacctaatat tttatttttt ctattaacca
    25921 taatcctagc ttgccatttc ttagacggct tatggaaata aaccccagta tgcccgctag
    25981 tattattttt aaacttccct ctatttttca tattttcagc atgtgtaaca tctctcaaat
    26041 tagatattct attatctgtc ttatccctat tgatatggtc tattgaagaa gcgtgtttcc
    26101 cataaacaaa aagccaaatt aatctatgtg cattatattg tttcttaaat atttttaact
    26161 tcaaataccc caacctgcct ttgtataaga aaacctcctt gccggaaaat ccggtattcc
    26221 aagatttgta atgcaaaccg ctattgaaat actttcttgg acgctccgcc caatataact
    26281 ttcccgtatt gtcatcatat cttaataatt cttttaaaac ctcttgtgtt aatcccataa
    26341 aacaccatgc tccatagatg cccaagatat aatcttttct tgatattcag acatttcccg
    26401 aactgataat tttgtcgttg atatacccat taattgcccg ttaggcattt caatacatcc
    26461 aataaatttt ctttttaaaa attcatgcca tacatcttga ctaaaccttc tcccatcgat
    26521 aaaaactttt tctgaaattt cacgatacaa aaaccatagc cgcctgttct gctcgatgct
    26581 ccgttttgac ttgcgcgggc ggattgtgat ttcaaggttg ccgttttcaa accacccgtc
    26641 cgggctgtcc caaatcgacc gcataacctg ctttttgttt tggggcgtca gtgtaaattc
    26701 cgccccgttc atttcagacg gcctttcacg ccgacaaccc ccagtgcttc aaggcggcgt
    26761 atcgtgcgca attgcgaacg gcgcatataa aattccttgt cttcgcggct taggcctgcg
    26821 cccctgccgt cgatcgcgcc gcggcggcaa ccgcacccga aacccgcgcc caaaccgccg
    26881 cttttcaatc ccgccccgtg cgtctcgccc gggaaacggc acaacacgac cgtttcaggg
    26941 ttgtcattgc acacgcccgc gatgttcggc gtgcattgcc cgcctttggc ggctttgcgt
    27001 atcgcgctag acatcccaac cctcctgtcc gatgaaatcc caaccctcgt ctgccgcatc
    27061 cgtctgttcc atctccaact cccccac
//
LOCUS       DAXDZG010000021        26748 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXDZG010000021 DAXDZG010000000
VERSION     DAXDZG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26748
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.021"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(54..608)
                     /locus_tag="ACFB78_000797"
     CDS             complement(54..608)
                     /locus_tag="ACFB78_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268834.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFE
                     LLIDEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMK
                     EIFDEILRIENLDRKNLPSNIMVK"
     gene            complement(695..1744)
                     /locus_tag="ACFB78_000798"
     CDS             complement(695..1744)
                     /locus_tag="ACFB78_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC1268835.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEKFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
     gene            complement(1936..3453)
                     /locus_tag="ACFB78_000799"
     CDS             complement(1936..3453)
                     /locus_tag="ACFB78_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268836.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVELARNNLRKFYSKR"
     gene            complement(3453..4196)
                     /locus_tag="ACFB78_000800"
     CDS             complement(3453..4196)
                     /locus_tag="ACFB78_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268837.1"
                     /translation="MDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            complement(4205..4810)
                     /locus_tag="ACFB78_000801"
     CDS             complement(4205..4810)
                     /locus_tag="ACFB78_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268838.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            complement(4800..6290)
                     /locus_tag="ACFB78_000802"
     CDS             complement(4800..6290)
                     /locus_tag="ACFB78_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC1268839.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQ
                     TSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLH
                     EEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDKYDEYTNCGIG
                     NKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            complement(6287..7174)
                     /locus_tag="ACFB78_000803"
     CDS             complement(6287..7174)
                     /locus_tag="ACFB78_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC1268840.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            complement(7415..7597)
                     /locus_tag="ACFB78_000804"
     CDS             complement(7415..7597)
                     /locus_tag="ACFB78_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268841.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            7597..7932
                     /locus_tag="ACFB78_000805"
     CDS             7597..7932
                     /locus_tag="ACFB78_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268842.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(7969..8628)
                     /gene="ung"
                     /locus_tag="ACFB78_000806"
     CDS             complement(7969..8628)
                     /gene="ung"
                     /locus_tag="ACFB78_000806"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC1268843.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            8709..9116
                     /locus_tag="ACFB78_000807"
     CDS             8709..9116
                     /locus_tag="ACFB78_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC1268844.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            9131..10078
                     /locus_tag="ACFB78_000808"
     CDS             9131..10078
                     /locus_tag="ACFB78_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC1268845.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(10185..11255)
                     /locus_tag="ACFB78_000809"
     CDS             complement(10185..11255)
                     /locus_tag="ACFB78_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC1268846.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            11440..11715
                     /locus_tag="ACFB78_000810"
     CDS             11440..11715
                     /locus_tag="ACFB78_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC1268847.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11717..12340
                     /gene="lipB"
                     /locus_tag="ACFB78_000811"
     CDS             11717..12340
                     /gene="lipB"
                     /locus_tag="ACFB78_000811"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC1268848.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            12333..13316
                     /gene="lipA"
                     /locus_tag="ACFB78_000812"
     CDS             12333..13316
                     /gene="lipA"
                     /locus_tag="ACFB78_000812"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC1268849.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            13791..14255
                     /gene="bfr"
                     /locus_tag="ACFB78_000813"
     CDS             13791..14255
                     /gene="bfr"
                     /locus_tag="ACFB78_000813"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC1268850.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARSELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            14283..14756
                     /gene="bfr"
                     /locus_tag="ACFB78_000814"
     CDS             14283..14756
                     /gene="bfr"
                     /locus_tag="ACFB78_000814"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC1268851.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            15084..15404
                     /locus_tag="ACFB78_000815"
     CDS             15084..15404
                     /locus_tag="ACFB78_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1268852.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            15453..18011
                     /gene="glnD"
                     /locus_tag="ACFB78_000816"
     CDS             15453..18011
                     /gene="glnD"
                     /locus_tag="ACFB78_000816"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC1268853.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            18158..19621
                     /gene="guaB"
                     /locus_tag="ACFB78_000817"
     CDS             18158..19621
                     /gene="guaB"
                     /locus_tag="ACFB78_000817"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC1268854.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVTVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(19957..22332)
                     /gene="rnr"
                     /locus_tag="ACFB78_000818"
     CDS             complement(19957..22332)
                     /gene="rnr"
                     /locus_tag="ACFB78_000818"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC1268855.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            22421..22507
                     /locus_tag="ACFB78_000819"
     tRNA            22421..22507
                     /locus_tag="ACFB78_000819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22455..22457,aa:Leu,seq:cag)
     gene            22546..22636
                     /locus_tag="ACFB78_000820"
     tRNA            22546..22636
                     /locus_tag="ACFB78_000820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22580..22582,aa:Ser,seq:gga)
     gene            complement(23033..24844)
                     /gene="typA"
                     /locus_tag="ACFB78_000821"
     CDS             complement(23033..24844)
                     /gene="typA"
                     /locus_tag="ACFB78_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC1268856.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(24961..25851)
                     /locus_tag="ACFB78_000822"
     CDS             complement(24961..25851)
                     /locus_tag="ACFB78_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC1268857.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            25916..26572
                     /locus_tag="ACFB78_000823"
     CDS             25916..26572
                     /locus_tag="ACFB78_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC1268858.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTACFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
BASE COUNT         6987 a         7210 c         6073 g         6478 t
ORIGIN      
        1 aaaaaaacca tcacaaaagt gatggcaaat gatttttaag gctattaaat atcttactta
       61 accatgatat tactaggaag atttttgcga tccaaatttt caattcttaa aatttcatca
      121 aaaatttctt tcatcgaaaa caattctgcc aagtctccgg caatctgcct acctcggcaa
      181 aaatactgtc tgcaatcaaa ataaaccttc atatgtaata accgttcatc aaggctggca
      241 ttcgtatcat ccaacgccgt actaatatcg gcccatttaa aaaacttctt tctcgacata
      301 cggccactgg aagaaacaac atttttgaaa aaaatcatat tcggaatata gtattttttt
      361 aaatccaaac cagccaaact tttattactc aatttcagat gttctcttac tataggatat
      421 atgagattaa ggccctgaca tgcttcatga aacgactgag cttcatcaat taatagctcg
      481 aaatcaacca atttcaattt aatttctttt tctaaatcct caatccccct tacccttaaa
      541 agccttttgt attcccaaaa ttttaagtat tttttcatac ctgatatttg agtattcata
      601 gtattcatat cacatcataa aaaaatccat acaaacaagg gccaatcaat accatcttaa
      661 aactcgattg taaccccacg ttccaatttc acaactagcc cgataatcca ttgtcacgaa
      721 caatatcaac caacttattg agatcctcct gattcatttt tctctggata taaaccaaag
      781 agggttgtga ttcatacacc caaacagaca atggaattct aggcagaatc tcttgctcaa
      841 cagcagattt caacttcgac aactgctctg ggtcaagcag actgtcacgt cgaatgctct
      901 tgatagccct ggtctccatt accgcaatat ataaatcatt cagtagcttt ctcatcatcc
      961 gaccgccatt atacaacgtg tcattacatt gtttattagc catcatgcct ttgatattaa
     1021 gtgtcttaac ggcacataca aaactatcgt aatcccgcaa caatttaacc agtttgaaac
     1081 cataagcccg caatacattc aatctgatat gaacagggtt attgttctga atcaaggtta
     1141 tcttcatacc tgcagcggcc aaactatcaa tctgagcatg aacctgtttt ttcacagtct
     1201 caaaaaattt ctcaatttcg gacatctgtt tttcaatctg caataaccga ctttgggcat
     1261 aaggattatc ggacatagcc ataccaaaca actcgttact gagtgtcaga ccatatctga
     1321 ttcccggcca acgtgggccg ttcttcttgt tgttattgcc ctcccacata cggatacctt
     1381 gcttggtatg cataaaaaaa ccatcatcac cggaagaggt caggctgcca atatttttaa
     1441 accggtcgtt ctcagaagaa agaatttcat actcccccca aaccaagtga tgctttcgac
     1501 ctgtctcctt ttgtagatgt tgcttgagca gcaattcatg tgctttaaac tcttcccact
     1561 gttggaactg ttcgtcatcc tcagtcaact tttgattgac cttatccccc aaagccaaag
     1621 cagcacgagc catatcgtaa ccgtcactga aaggtgactg ttttgttgtg actttgagat
     1681 caaattgctg tgcaacatgg atcgcaccag gtttgatgat atatctttgt tttgcaaatt
     1741 ccatttttaa atccttataa gttattaaaa agagcttcat aaagcactgt tgttgcatat
     1801 cagaggctaa ttagtcatgt acaccatcat atctgtcctt tgccgatgac tgtaacgccc
     1861 cgttacatcc gcttcgaagt aatagattta ttcgtctttc aaaaattcat caaaaatatt
     1921 tttttaaatt aaattttatc ttttggagta gaactttctt aaattattgc gggcaagctc
     1981 gacatccatg gcatctaatg agtcctgttt ttggttattc tgcttttcaa gataagcctg
     2041 taatttctga tcctgctcac gctggaatct aacctgtggg gcgacaagag cagtaaagtt
     2101 accttgcgaa gcgcagcggc ataggttaat caaaagacca agggggctag taacttcttt
     2161 tttctgtccc tggatctcat ccaagatttc ctgcctcaaa gcccaggaac tttttgagag
     2221 atgttttgta aaaatcaaat ccgcctgttg acgcatttgc acattaaaaa gttttggata
     2281 aatcaaatca acatcatcaa ccactaccac atgatcttgt tcggaagaac gtggtttgtt
     2341 gggttcaaca gtcatgtagt tgtagttata aacccctttg taatttatat aataaataat
     2401 ctgccgattc ggcaattctg aacttcccca atcgacactt atgaatttcc cactacgacc
     2461 tttcttaaaa ttccaattcg acacatccga atattccctt tcgacacttc cggatgtcat
     2521 acttgggaat aggggggcat caccctcgaa accgctctga aaccctttat ccaatccatt
     2581 tggccctttc cgccaatcac atttggccct ttccgccaaa ctgagaattt cgcgtttttt
     2641 ggagttatat ggactaaagc cgtcgtcctg tttgccagct agaagttttt tgatggatat
     2701 aacaatatcg tgaccattta ccagggtaaa aatacggcgg ctggccaaga aatttctctc
     2761 ataaatgaga tttaaattct tcagcctatt gcgagctgtc aattgagatt tataagagag
     2821 agaggttctt tcctcccaat cagcagcagt caaagtagca aaggcatgac gttgtaaatg
     2881 tggattttga gaaaccaaac caaccgcatc atttaacaca cacgacaata atacagccga
     2941 gttgatacag cctgtaatag aaaccaggtc tcggtgataa gcaatccttt tagaaaagtg
     3001 ttttctaaaa tacagggaac ttgtgttctg taaatcagcc agcgtaagtg caagttgatc
     3061 gtcagtaccg ctcgatttag cgatcaactc agctatggca tccaagttca ctttgattgc
     3121 taaagcagcc cccacacctt tacggcatgt ttgaataagg tttgtctcaa gaagcttgtt
     3181 tttacaagtg cgctgctctc tcactgtcaa accagtttca gcttccatct gagcaataga
     3241 cttgaaaatc caaccatccc gttcggcaac ctctttgctt actcttgtcc aatagattaa
     3301 ctgagaaagc attgcagcag tcttcagaga gcctgtaata tcaattaaac atcgatagat
     3361 ggcaaccctt cgaccacctt gcaatgcttg ccagcattgt gtcagttcag agttaaatac
     3421 tttgggaggg ggtaatttgg tggcggtaac catcacacac atccgttcaa atattcagaa
     3481 gtaagatcag caagcatttt caagctgtat ctacaacgtc gcgctgctgg cataccatca
     3541 acacagctct caatcaaacc aaaagaggtt aattcggcaa tagctctttc ctgttgccgt
     3601 cttgacaaca ttgttgaata aaaaacgtgc ttttcagctg catgagtcca aggagaccag
     3661 tcaccacgct tactcaaatc ttgatattca gcttcacttt gaagaaacga caaatacaaa
     3721 atggatacaa aagcaagacc tctatcggct aatgtcttca ggtgaagttt attaatcgaa
     3781 atagggggcg aaaccatgtc aagtacatct gatttaccat gtttacttac caaatcctca
     3841 aaaagagcat cattcaatgt gaattgtgat tcggttggcg ctacggttct ccgattaagc
     3901 aaaatacctt tctgcagaag gctattgcga atattccgcc actgctttgc ggtaatacct
     3961 gcttcattaa acaacagatc attattcagg cttacccatt cgccaggcag acatttctcg
     4021 acaaaccaac tcaaaaaata tccctctgaa acagaaccga ctgcctgagc aaactgacgc
     4081 acatatgtgg ccggttcctc ccacaaggca accaagctac ggataaagtt atcggttgac
     4141 atgttcttag atggtacaaa accagtctca taatcatagg aagtagagaa atccatatac
     4201 atactcatac tccaaagaac tttacatcgt taattattga atgtaattgg gcaaggctat
     4261 aatcggggaa ttcatcaatc aatagctggt atctaataaa caaatctggc tctttttccg
     4321 acttactcca tgccttcaat accctgacgc gaacatcaat agatggcatc acaatgcgcc
     4381 ctttaacgac aggaatattc atttctatac gcaatttttt aatttccgcc gtagaaatta
     4441 atgaacatag attcataatc atcagattgc tggccttatg ttctaacatc catttaagac
     4501 gctgcatctg ttccataaac ctttgttcgt aatgttccat ttctgaaatt ttgcttttta
     4561 aaaccttcag atcaatggag taggtcacaa catcaggaca catggatgct aatttctcaa
     4621 tccgcttcgg gttcattcgt ttgagcgtgt tcaaaacatc ctcatgttca cgagcaacca
     4681 aagcgttata agaagattca cgcggcgtat ttggtgcgga tgccaacctt gtaagtgcaa
     4741 gattggtata aaccggatcc gtattaaaca gtctataaat gggattacta attaaaggct
     4801 tactcctcac tcttagctcc cgtttttctc aagttgttgt actgtgtgct cagagataat
     4861 atttctacga tattggcgtg aaattcatgg ctggtattag tttgccaatt cagtaaagag
     4921 gcaataatgt tctctttatt accaattccg cagttcgtat attcgtcgta cttatcgggg
     4981 tcctcgtatg caatacgaag cttagaatca cttttaagtg tcagcagggg attggaacta
     5041 tcaccaacat caaacataaa ggtgtcgatt ccactaagct tccataagaa tgtgaaaaca
     5101 tttcccgctt cttcatgcaa aacatcaata gcatagatgg catttccggc acttgatgct
     5161 gaaatatgct caaaagccgg atattcgaca taccagccgt atggaaagtc cttatgaatc
     5221 ttaaagcaat cggacagact cactttggct aacagtttac gaacagcaga aaacagtttt
     5281 ttgctggttt gctccaaagt aatcggttcg gcttctttcg gctcatcctc tgcatcagct
     5341 ttagtaccat ttactttggc tggcacaaat tccatatccg aatccttttg atgcaaattg
     5401 acatcttgtt tggcggctgt ttcaattttg tctttatcag aagaattttc tgtttcagaa
     5461 aaggcgtcag agccatattc aaatttaaat ctgtcgagca cgaactgata aagctcatgc
     5521 acgtctaact ctgttgcaga atattggtta tgccactgct ccagtacatc cgaccacaaa
     5581 tattggaaag aatcgtccaa ctgaattgat ttagaaattg agaatagggt agagtgaagt
     5641 tttttaattt cttctacttt tgcatgggaa agtttgaatg cgtattcttg cagctcttgt
     5701 aaattatcgg aggcaaaaaa gaataatcct aaagtcttat gagaaaccgt aaggccttta
     5761 tcagaaaaca tcacttccag ttcgcgcaaa gaaagtttcc ctgagtcaga ttcatcttgg
     5821 aaaatatcgc ggatttcggc atatttacag gccttatccc aaaaacacat ttcagcacgg
     5881 agttcatttt caacaagatg cgcaacctgc aatgtagttt cgtcagagta ggggatgtaa
     5941 atacaaggca tcatacggaa gcggttatca ccagtttctt ccaagagttc gcacaagatt
     6001 ttgagacggg tattaccacc ctgtgccagt acatatttac tttcaccagg tctctgggta
     6061 acgtgtacag gttgttgaat acctgaagaa cggatggact ctttgatggc cgcgtagctg
     6121 gaaacatcat gtaaacggcg agggtttttg tcaaagaaat cgatttcgct gattggcaac
     6181 gacattaaca tgccccgaga aggagtatcc tcttggccaa aactcatatt tgtctgtgca
     6241 ggggtaccga tgtgcaggct caaggttgct ttattttggt ccaaattcat gattgcacct
     6301 ccttttgcag caggctggcc atgtcattga aacagcttcc acgcaggttc ttctgttcga
     6361 taccagggaa aatctcataa acaatacggt gcatgatttc ataagcaggc agcatctttc
     6421 caagctgttt acgctcatga caatgaaccg ggactcttaa agttgtagct tccttatatg
     6481 ctttggcagc aggaactgct acgcttaaca atttacgtct gtcatctaga gtattgctaa
     6541 agaaatcttt gatagaagat gcgatgaatt tcgcatcatt ggtacgatct tgtgcgtaga
     6601 tgatggctcg taattgaggc atcgggatac gcattgcttc gcctttttgc agacgcttta
     6661 gaagttcttg cgtaccggta aggaactcac gggcactcaa aacttcgggc ataattgggc
     6721 aaagcaggca tgtagcagca aatgctgcgg tatctaagag aggaccaact gcaccctgag
     6781 tatcgattaa aaccacatcg tagttattct gtatgtattg ggaatgcagt ttgcttttga
     6841 gcaagaaggc acggtcggga cgagaggtta cttcagtctc gacaaaagca gaaatgttat
     6901 tggaaataac aatgtctaaa ttcggaaaaa cggtattgga aattgtcgag cggatgattt
     6961 cttcggtatt ttcaccgaga agtaattcaa caataccatt tggagcgcgg tggcttattg
     7021 gatagtattt tgacaatgat ggctgaacat cgcagtcaat cattaagaca cgcagtccga
     7081 tatcagcgag aacagcagag acattagcaa tgagtgttgt tttacctacg ccaccttttg
     7141 tagcggctgc agacaatatt acgggtgcgg acataatatt tccttatatt taattgattt
     7201 ataaagagaa aaacatccgc aatcgaacag gtttaaaata tgaaatttac atatttattc
     7261 tagttcgcat aatgtatatt atgttaaatt tataatggat tgaatagata tttttacaat
     7321 ctcattcaaa actcagacac ttaaaaaatt taatcagaac aaataaaaaa gcaaacaaaa
     7381 atatccatct ttcaaactca tattttgcaa taacctagat aagcaagaac aggggcagaa
     7441 cggattcgcc tttgatgtgg tggtaggcaa ccattccaat atgtcccagc gacaaaaaca
     7501 gcaatgaaaa tgccagcgtg ccgtgaattc ctccgccatt gccgatttga cgggcaatgc
     7561 ctgcggcgga acagcgagca tcagggcata cgcccaatgc ccaatttggt gacggcggtg
     7621 ttggcggggc ggtttttacg ttggcggatt ccctacccta cgcaccaaac agacaatatc
     7681 agcagcagga ttctgactgc acggatatgt tggtatgaat cgtccgccca agcggaaaag
     7741 gctgtcgcgc ccaaaacagt ggcaagaata aattgcttga atttcatgat gttgcttctt
     7801 ttgcacaaag cgttcggaat agtgaagccc gcagcctatg gctttgcggc agtcgcagct
     7861 ttacaagcgg tattgcagga aaccttaaaa cacgatcatt gcatcaagag ctgttcgaat
     7921 tccgccaaat gaacctgaca cgcaaacttt gacgcttgaa tacggcattc atagcttcca
     7981 gtttatcggt tcgataccgt gttgactcaa atagctgttt gcctgtgaaa aatggcggca
     8041 gccaaaaaaa ccgcgatatg ccgacagagg agacggatga ggcgcggtca gtatcaaatg
     8101 attttgactg tctatcagcc tccctttttg ttgtgcatac ccaccccaca acatgaagac
     8161 aaggtgcttg cggtgtgtcg caagctgcct gatgacggta tcggtaaagc gttcccagcc
     8221 taaaagggcg tgcgaatgcg cctgtcctgc acgcaccgtc aaaaccgtgt tcagaagcaa
     8281 tatgccctgc tccgcccacg ctgtcaggca gccgtgcgcg ggaatggaaa agccttcgat
     8341 gtcggtttcc aattctttgt aaatattgag taaagacggc ggtatgcgga caccctgccg
     8401 gacggaaaat gccaaaccgt gcgcctgccc taccccgtga tacggatctt gtccgagaat
     8461 gacgactttg acccggtcga acgctgtcag ccggaatgcg ttgaacacat cggcctccgg
     8521 cggatagata atttgccccg acaaacgttc ctgcctgacc gcatttaaaa tttcctgaaa
     8581 atacggctgc tgtttttcgc cgccgagtgc atcgtgccaa gtgtccatat ctgccctatc
     8641 ctttcaaatt cggctatact gctgatattg cgttattttg tcaaacgacg cgctttgtgg
     8701 ggaaatgtat gactgtatgg tttgttgccg ctgttgccgt cttaatcatc gaattattga
     8761 cgggaacggt ttatcttttg gttgtcagcg cggctttggc gggttcgggc attgcctacg
     8821 ggctgactgg cagcacgcct gccgccgtct tgaccgccgc actgctttcc gcgctgggca
     8881 tttggttcgt acatgccaaa accgccgtgg gaaaagttga aacggattca tatcaggatt
     8941 tggataccgg aaaatatgcc gaaatcctcc gatacacagg cggcaaccgt tacgaagttt
     9001 tttatcgcgg tacgcactgg caggcgcaaa atacggggca ggaagtgttt gaaccgggaa
     9061 cgcgcgccct catcgtccgc aaagaaggta accttcttat catcgcaaac ccttaacact
     9121 cggaggaatt atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa
     9181 atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg ggcgtttcca
     9241 tcgcgccctg acggccggtt tgaatatttt gattcccttt atcgaccgcg tcgcctaccg
     9301 ccattcgctg aaagaaatcc ctttagacgt acccagccag gtctgcatca cgcgcgataa
     9361 tacgcaattg actgttgacg gcatcatcta tttccaagta accgatccca aactcgcctc
     9421 atacggttcg agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc
     9481 cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa tcaacagtac
     9541 cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt gtgaaagtcc tccgttacga
     9601 aatcaaggat ttggttccgc cgcaagaaat ccttcgcgca atgcaggcac aaattaccgc
     9661 cgaacgcgaa aaacgcgccc gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa
     9721 ccttgccagt ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc
     9781 ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag gcgaagcgga
     9841 atccctgcgc cttgttgccg aagccaatgc cgaagccatc cgtcaaattg ccgccgccct
     9901 tcaaacccaa ggcggggcgg atgcggtcaa tctgaagatt gcggaacaat acgtagccgc
     9961 gttcaacaat cttgccaaag aaagcaatac gctgattatg cccgccaatg ttgccgacat
    10021 cggcagcctg atttctgccg gcatgaaaat tatcgacagc agcaaaaccg ccaaataagc
    10081 cgtgatgaaa ataccgtctg aagctctgtt ttcgggtttc agacggtatt tttatttaaa
    10141 cccgaacagg gttgtattcc gcttcaaata tgtttcggaa ccgtctacct gccccggtaa
    10201 aaactgccgg caaaatattt ctgcgcgccc tcgcgaagca agaccaaggt tacggcggcc
    10261 aaaggcaatc cggccaacat tccgacaaag cccatcagct ctccgaacgc catcagcgaa
    10321 aagataaccc aaaacggcga caggccgata cggtctccta caattttcgg cgtaatgaaa
    10381 aaactttcga gaaactgacc gacggcaaaa accgcccaaa cagccaagat tccgttccac
    10441 gaaccgaact ggagcaaggc tgcaacagtg gcaagcagca atcccgtaaa cgcacccaaa
    10501 taggggacaa acaccaaaat accggcaacc ataccgatgg caaatcccga atccagtccg
    10561 actagcatca atcccaaacc gtaaaccaag cccataatca gcatcaccag aagctgaccg
    10621 cgcaaaaatt cgcccaatac ctcgttcaaa ttacccgtaa tgcgcgtata agcaccggca
    10681 aaacgcctcg gaaccagttt ggcgatgccg cacgaccacc gctgccaatc cagcaggaaa
    10741 tagtaaagca gcaagggcgg cagcagcagg ttgccgatac tgctgacaat attgccgccc
    10801 tgtttcatca aaacgggaaa ccacgcctta agcgcgttgc tcaactcgcc cgtatgcgcc
    10861 tgaaaccacg caataataga tgcctgatcg atttccacat atccgccgat tgtatttttc
    10921 aaccacggca gcagcgtgtt ctgtataaaa ccgattaatt ggggcaggcg agatgccaaa
    10981 ttattgaact gcccgaccag catagggaca ataatcaaca ataatgccaa caacaaaatc
    11041 aaggaaaaca ccatcacaga catcgaagcg gatgcacggt tcaaaccctt tttctgcaac
    11101 cattcgacca aagggtccaa cacatacgcc agcaccgccg caaccgcaaa cggagtcaaa
    11161 gtatcgccga gcgcgtaaac cagccagacc aaggcggcaa acgccgcgcc ggcacccatc
    11221 cacggcttga tgccccgtcc ttttctccga tacataaaat ccacccttcc gaaaataata
    11281 ttgcgcgcca gtatagcaga accgccgcgc cgtcaaaaaa gcctccgcct tcccgatagc
    11341 ctgttatcgt ggtttaggct aaaatactgc acatccgatg ccctctgaaa ggcaaacccg
    11401 ccttcagacg gcataccgta cacgataagg cagcacatca tgaccgaaca aaaaaacaaa
    11461 gcatccctca tcgaatttcc ctgcgccttc ccattgaaag tgatgggcgc ggtgcatccc
    11521 gagttcgagc aggcggtatt ggacaccgtc cgccttcacg cccccgacac gcaggcgcac
    11581 cacatcacca cgcgtccgag cagcaaaggc aactataccg gtgccaccgt tcaggtaaag
    11641 gttgaaaacc aagaacaatt ggacaacatc taccgcgcgc tgacttcgca cgaactggtc
    11701 aaagtggtac tttgagatga aaatcataca caaaggtttg gtcgaatatc tgccgacttt
    11761 tgaagcgatg aaaaccttta atgccggacg gaatgcagac accgaagacg aattgtgggt
    11821 cgtcgaacac ccgcccgtct ttacgcaagg actggcggga aaacccgagc acctgctgat
    11881 tagggacgac atccccgtcg tccaaatcga ccggggcggg cagattacct atcacgggcc
    11941 cgggcaattg gtcgtttata cgatgattga tttcaaacgg cgcaaaacca gcgtccgcaa
    12001 catcgtttcc gcgcttgaaa acagcatcat cgccacattg gcggaatacg gcatcgaagc
    12061 ggcggcagat cccaaacgcc ccggcattta cgtcggcgaa cgcaaaatcg cctcactggg
    12121 gctgcgtatc aaaaacggtt ccgtctatca cggactcgca ctcaacgtca atatggattt
    12181 aagcccgttt acccaaatca acccctgcgg ctacgccggt atggaaatga cgcaaatcgc
    12241 ggattttgtc caaccctgcc ccgcgccgga cgaagtcgcc tccaaactca ccgcacacct
    12301 tgaaacacaa ctcacaccga aagcagacaa caatgagtga aatcaaaacc gacgacccca
    12361 aacgcggcat caaactcaga ggcgcggaca aaaccgcccg catccccatc aaagtcgtcc
    12421 cccttcagga aaaactgaaa aagcccgaat ggatacgcgc caaactccca tcgcgcaaat
    12481 tctttgaaat caaagacatt ttgcgcgaac aaaagatgca caccgtttgc gaggaagcct
    12541 cctgccccaa catcggcgaa tgcttcagca aaggcacggc gaccttcatg attatgggcg
    12601 acatctgcac ccgccgctgc ccgttctgcg acgtgggaca cggccgtccg aatatgctcg
    12661 accccgacga gccgaaaaac ctcgcagaat ccgtcaaagc catgaacctg cgttacgtcg
    12721 tcatcacctc ggttgaccgc gacgacctgc gcgacggcgg cgcccaacac ttcgccgact
    12781 gcatcaaagc catccgcgaa accagcccga acaccaaaat cgaaatcctc gtccccgact
    12841 tccgaggccg cttggacatc gcactgaaaa tccttgccga aaccccgccc gacgtgatga
    12901 accacaactt ggaaacccat ccgagcctgt acagaaaagc ccgtcccggt gccaattatc
    12961 aacattcttt agacttatta aaacgttata aagaaatgat gccgcatatc ccgaccaaat
    13021 ccggcattat ggtaggcttg ggcgaaacag acgaagacgt gcgtgaaatt atgcgcgata
    13081 tgcgggcgca caatatcgag atgattacca tcggacagta cctccagcct tcagacggac
    13141 acttgcctgt cttgcgctac gttacacccg aacaattcaa aatatttgaa aaagaagcat
    13201 acgaactggg ctttaccaat gccgccatcg gcgcgatggt acgttcaagt taccatgccg
    13261 acgaacaggc ggcggaagcc ttacgcgaaa gccacggcgg ttgcgggcat cattaaactc
    13321 ggaacaccgt tccgaagtaa aaaataattt cctacgctgc tgtctgccgc cttgtttgat
    13381 gtaaacaaca tcaatttgaa ttgtccatgc cgtctgaaag tccttcagac ggcataattt
    13441 attcccaaaa tccgataatc tcaaaagaat ctgtcggtta atgagaaaga ttcattataa
    13501 attttgataa aaaaccgtgc tctatttgtt ttttgctatg gttaacaagt gtaaatgttc
    13561 tttattgatg agatggattc ttagttttag cagcaaaaaa aaccgataag aaacagcata
    13621 ctgatttgga aggcatcggg gaatacgccc tatttatttt gataattgtt tgtattttaa
    13681 atagaaaatt tgtcatgccg acagcttttg catacaatgc acgcatcccg aacagcgtta
    13741 cagaacgccg tttaaccgca cgaaacccaa attaagaaaa gagagatatt atgcaaggca
    13801 atcaagctgt tgttgattac atgaacgaat tgctgtccgg cgagctggcg gcacgcgacc
    13861 aatacttcat ccactcccgc ctctactccg aatggggcta caccaaactt tttgaacgtc
    13921 tcaatcacga gatggaagaa gaaaccacac acgccgaaga cttcatccga cgcatcctga
    13981 tgctgggcgg tacgccgaaa atggcacgct ccgaactgaa tatcggcacg gacgtggttt
    14041 cctgcctcaa agccgacctg caaaccgaat acgaagtgcg cgacgctttg aaaaaaggca
    14101 tcaaactgtg cgaagaggct caagactatg ttacgcgcga cctgatggtt gcccaactga
    14161 aagacaccga agaagaccac gcacactggt tggaacagca gctgcgcctg atcgagctga
    14221 ttggcgaagg caactactac caaagccaac tgtaatccgt attaggaata aaaggagtcc
    14281 gtatgaaagg cgaccgtttg gttatccgcg agctgaacaa aaacttaggc ttgctgctgg
    14341 taaccattaa ccaatatttc cttcacgccc gtattttgaa aaactggggc tttgaagaac
    14401 tgggcgaaca tttcttcaaa caatcaatcg tagaaatgaa agctgccgac gatttgattg
    14461 aacgcatcct cttccttgag ggattgccca atttgcaaga gctgggcaag cttctgatcg
    14521 gtgaatctac cgaagaaatc atcgcctgcg atttgaccaa agaacaggaa aaacacgaag
    14581 ccctgcttgc cgccatcgcc acagcagaag cgcaacagga ttatgtcagc cgtgatttgt
    14641 tggaaaaaca aaaagatact aacgaagaac acatcgactg gctcgaaacc cagcaggaac
    14701 tgatcggcaa aatcggtttg ccgaactacc tgcaaacagc ggcgcaagag gactaaaaca
    14761 caaaccactg ccaaatacag cagtcccctg catcatcaga cggcatatcc gtataaacgg
    14821 gtatgccgtt tttctttgcc ggtttacgaa taaaatctaa attgatttga atcaatactc
    14881 gataacggtt tttgtttgat aataccgata tgaaatctag attgtttgtt tgctgtttcc
    14941 caatccgtct tgattttacc tctttcctct tgatgtgtgt gtgtgtttgg gtgtggctgc
    15001 cgccacccct tttttttggc ttttatgtga agtaaaatcc gtaacagcaa atgcttaact
    15061 tttaaccatt tatccggaaa caaatgatgg gcaacaaatt gactctgcct gccgaattgc
    15121 ctgacgaaca ggatttgcgg gcggtattgg catacaatat gcggcttttc cgcgtgaaca
    15181 agggttggtc gcaagaggaa ttggcgcggc aatgcggttt ggacaggact tatgtgtcgg
    15241 cagtcgagcg caaacgctgg aacattgccc tgtcgaatat cgaaaaaatg gcggcggctt
    15301 tgggcgtggc ggcgtatcag ttgctgctgc cgccgcagga acggttgaac ctgatgacca
    15361 attccgccga tacccgacaa gtgccgtctg aaagcggtat ttgaccgccg aaccatccgc
    15421 ctcccttttt cccgccaatc gaaagaataa aaatgcctga aaacctgtct tcagcactgg
    15481 aaaccttcaa acagcagcgc aatgccgccg aagcgcatta tttgaaagcc aaccgcgtgt
    15541 cggtattttt cagagaatac acggcggcag tcgaaacctt gctggcggca ttgtgggcag
    15601 aacattttca aaacagcgcg ttatgcctga tggcggtagg cggcttcggg cgcggcgaac
    15661 cgtatccctg ttcggatgtg gatttggcgg ttgtctcccc tgccccgctt tcagacggca
    15721 ttcaggaaca gattgcccga tttatccaaa ctctgtggga ttgcaaacta atgccgtctg
    15781 taaaaagcgg cagcgttgac gaactgtgcg aaagcgtgcg cgacgatatt acgggggaca
    15841 cagcgttttt agaggctagg tttttgtttg gcaaccgcca aacagcagac gaattggcgg
    15901 aaaaaatgaa cgtgcaacgc aatgtagcgg catttatcga agcaaaattg gtggagatgg
    15961 aacaccgcca cgccaaatcg caaggttcgg gggcggtgtt ggagccgaat atcaaaagct
    16021 gtccgggcgg tctgcgcgac atccacaccc tgctttggat agcgaaggcg caaggcttgg
    16081 cggccaacct gcccgacctg ctcaaacagc ggattttgac acgtgccgaa gccggtatgc
    16141 tttcgcacgg ctaccgccgc ctcgcccaca tccgcatccg gctgcattta aacgccaagc
    16201 gcgccgaaga ccgcctgctt ttcgatttgc agccgcaagt tgccgaaagt atgggttatc
    16261 aagatgaaaa ccgccgccgt caaagcgaag aactgatgcg cgtgttttac cgtgcggtca
    16321 aaaccgtcaa acaactgggc ggcatcctca cgcccatgct gcgaagccgc gtttcctcca
    16381 cgccgatgcg cgttaccctg cggattgacg acgactacat ccaagtcaac aaccaaatcg
    16441 ccgcgcggca caccgatatt tttttcagac ggcccgaaca cattttcaaa atcgtcgaaa
    16501 tcatgcagca gcgcaacgac attactgcgc tcgaaccgca aaccctgcgc gcatggtggg
    16561 gggcgacgcg caaaatcaac cgcagcttct accaaaattc tgaaaaccgt cgccgcttcg
    16621 ccggtttttt ccgcagcggc aacggactga cccaaaccct gcgctttctc aacctctacg
    16681 gcgtgttggg ccgctatctg cccgcgtggg aaaaaatcgt cggcctgctt caacacgact
    16741 tgttccacat ctatcccgtg gacgaccaca tccttgccgt cgtccgcaac gtccgccgcc
    16801 ttgccctgga tatgcacagc catgagctgc cctacgcctc cgcgctgatg cagtcctttg
    16861 aaaaacaaga catcctctac cttgccgcct tcttccacga catcgccaaa ggacgcggcg
    16921 gcgaccatgc cgtacaaggc atcgcagacg cgcgccaatt tgccgccgac cacttcctga
    16981 ccgaagaaga aagcgacctg ctcgcctggc tggttgaaaa ccacctcctt atgtctgccg
    17041 tcgcccaaaa agaagacatc caagaccccg gcgtactcga tgccttctgc aaacgcgtcc
    17101 aaacccacga acgcctcagc gcgctctacc tcctgaccat ttccgacata cgcggcacca
    17161 atcccaagct gtggaacgcc tggcgcgcca gcctgctgga aagcctgttc cacgccgccg
    17221 gacgctgcct cgcaggcaac gacggcaacc cgcacgccct cttcggccgc cgccggcagg
    17281 aagccgccga cttacttacc cgcgccgccg tccccgaaaa acagcagaaa aaactatgga
    17341 acgcgctcgg ttccgcctat ttcgcccgcc atcagtcccg cgaaatcctg tggcacgccg
    17401 ccaacctcgt acacgacttt gaacccccca tcgtccgcag ccgcatccta ccccaaagcg
    17461 acagctttca agtgatggtc tttatgccca acggcccgcg cctgttcgcc cgcctctgcc
    17521 gcatcttcag ccgccacggc ttcgatatcc tcgccgcccg cgcctttatc accgaacacg
    17581 actacatcct cgacaccttc atcgtgcaaa tcccctcgca gcacgccccc gaagactacc
    17641 ccgacatcca aagcgcgctc gaagccgaac tcaacagctt tatccacgga cacaccgttg
    17701 ccgaaaccca aagctgcaac cgccgcatca gccgccgcag ccgctatatg cctattgcgc
    17761 caagcatcac catcaccccc gaagaagact atccggaccg gtattccgtc gaaatcaccg
    17821 ccgtcaaccg ccccttcctg ctcgccgaca tggcggaagt cttcttcgcc cacaacgtca
    17881 gcctgcgcta cgccaaaatc tccacgttgg acgaacgcgt cgaagacagc tttaccgtct
    17941 tcagccccga cttgaaaaac ccaaaaatcc agtcctcatt gaaacaggct ttgctggaac
    18001 agttggcata agcaccctgc cggttttcaa acggcatccc gccgccacgc cgtatgccgt
    18061 ctgaaggcgg gggatattgc aaacaatata aactgtcaag ccgccggcaa cgtgctataa
    18121 tgccgtgcat cttgcacaaa ccgaaagacc gagcattatg cgtatcgtag aaaaagccta
    18181 tactttcgac gatgttttgt tggttcccgc acattcgacc gtgctgccgc gagacgttaa
    18241 acttcaaacc aagcttaccc gcgaaatcac actcaacctc cccctgcttt ccgccgcgat
    18301 ggataccgtt accgaggcgc gcctcgccat ttcgatggca caggaaggcg gcatcggcat
    18361 catccataaa aacatgccgc ccgaaatgca ggcgcgcgcc gtttccaaag taaaacgcca
    18421 cgaaagcggc gtggtcaaag accccgtaac cgttgcgccg acaacgctca tccgcgaagt
    18481 cttggaaatg cgcgcgcagc gcaaacgcaa aatgtccggc ctgcccgtcg ttgaaaacgg
    18541 caaagtcgtc ggcatcgtaa ccaaccgcga cctgcgtttt gaaaaccgcg tcgatttgcc
    18601 cgtttccgcc attatgaccc cgcgcgaacg tctggttacc gtccccgaag gcacaagcat
    18661 agacgaagcg cgcgaactga tgcacacgca caaagtcgaa cgcgttttgg tcctgaacga
    18721 aaaagacgag cttaaaggct tgattaccgt taaagatatt ttaaaaacca ccgagtttcc
    18781 caatgccaac aaagactccg aaggccgtct gcgcgtcggt gcggcagtcg gcaccggcgg
    18841 cgacaccgac gagcgcgtca aagccttggt cgaagccggc gcggacgtga ttgtcgtcga
    18901 tactgcccac ggtcatagcc aaggcgtgat cgaccgcgtg cgttgggtca aagaaaccta
    18961 tccgcacatc caagtcatcg gcggcaacat cgccactgcc aaagccgcat tggatttggt
    19021 caccgtcggc gcggatgccg tcaaagtcgg tatcggtccg ggatcgattt gcaccacccg
    19081 tatcgtggca ggcgtgggcg tgccgcaact gaccgccatt cacaacgttg ccgaagccct
    19141 caaaggcacg ggcgttccgc tgattgccga cggcggcatc cgcttctccg gcgacatcgc
    19201 caaagccctc gccgcaggcg cgtacagcgt catgctcggc ggtatgtttg ccggcacgga
    19261 agaagccccg ggcgaaatcg aactctacca aggccgctcg tacaaatcct atcgcggtat
    19321 gggttccttg ggcgcgatga gccaaggttc tgccgaccgc tacttccaag acaaaaccga
    19381 cagcaccgac aaatacgtcc ccgaaggcat cgaaggccgc gttccctaca aaggtccgat
    19441 tgtgaacatc atccaccaac tgaccggcgg actgcgttcc agtatgggtt atttgggttg
    19501 cgccaatatt gccgaaatgc acgaaaaagc cgaatttgtg gaaatcactt ccgcaggtat
    19561 gagcgaatcg cacgttcacg acgttcaaat taccaaagaa gcgccgaatt accaccgctg
    19621 atttgaacag cctttcagga caaatgccgt ctgaaaccta atttccgagt ttcagacggc
    19681 atttttgttc gttcaaggca agccgcacgg gctgattgcg cggacgggca aagccattct
    19741 gacggacacg ccgaaagaat gaaaatgccg tctgaacgcc gtttgcaccc cgtctgccga
    19801 ataccgtcgg cctttaaaac ggcggtggaa ataaacgcaa tctttcaata cgggcgtttc
    19861 gattggtcgg aaatgatgcg ccggccgcca tatcaaaaaa tcgggcggcg gcttcgggaa
    19921 agtatgcccc gccctcccca agccgccccg tcagcattat gatttgcctt tccgtttttt
    19981 gaccttaatc ggaaccggtt tctttgcctt tttccccgat tcggcaacgg caggcacgcc
    20041 ccttcccctt actttgccgc atcgggctgt ttttttctcg gcggtggttt tcgatttccc
    20101 tttccccgcc gcccctgccg gtttggcaga tgcggataat ttgaccttcc gccgcctgcc
    20161 gctttctccg gcaattagga caaagtcgat ttttccatca tccaaatcgg cacgcgcgac
    20221 ccggacggca accctgtccc ccatattgaa acggatgccg ctgcgttcgc cttcgattgc
    20281 catgatttcg gggcggaagt tgaaataatc ttcgcccaaa tcgctgatat gtaccagacc
    20341 gtcgatatgg atatcgtcca aagtgacaaa tattccaaaa tttgccaccc cggagatttt
    20401 gccttcaaat atttcaccga ccttatcgcg catataataa gttttcagcc agttttccac
    20461 atcgcggcca gcatcgtcgg cacggcgttc gcaaaacgaa gtatgcacgc ccaaagcctg
    20521 ccagcttttg tttggcgtgt aggttttccg gttcaatacg gctttgatgg cacggtggac
    20581 ggtcaggtcg ggatagcggc ggatgggcga ggtaaagtgg gcgtatgctt cataagccaa
    20641 accgaaatgc ccttcgcaat gcggttcgta aaccgcctgc tgcatggagc gcaacatcat
    20701 gacttgcagc aattcggcat ccggcctgcc tttgaattgt tcggcaagcg cggcatagtc
    20761 tttcggcgac gggttgtcgc cgccgccaag ttgaagcccc aacagaccga gctgctcgcg
    20821 cagggtggcg agtttttcgg gcgtggggcc caaatggttg cggaacaaag ccgtatgttt
    20881 gtttttcaac agaaaatccg ccgcgcaaac attcgccgcc agcatacatt cttcaatcag
    20941 cttgtgggca tcgttgcgga cgacggggac aattttttcg attttgccgt tgtcgtcgaa
    21001 aatcatctgg gtttcgacgc tttcaaactc caccgccccg cgcgccagac gttttttctg
    21061 caaaatttta aacagcttgt aaagcgtgtc gatttgggct ttgtgcggat tcccgatgcc
    21121 gtctgaaagc catttccaaa cttggttgta ggtcaggcgg gcatgagagc gcatcacggc
    21181 gggatagaag cggtattctt tgatattgcc cgcataggta acgaccatat cgcacaccat
    21241 acacaaacgc tcgacatcgg gattgagcga gcagatgccg ttggacaggt tttccggcag
    21301 catcggaatc atacggcgcg ggaaatacac gctggtactg cgttcttgag catctgcatc
    21361 aatcgcatcg tcagggcgga cataatggct gacatccgca atcgccacga ccagacggta
    21421 attgcgtccg actttttcgg caaacaccgc gtcgtcgaaa tcgcgcgccg tttcgccgtc
    21481 tatcgttacc aaaggaaggt cgcacaaatc gacgcggcct ttcaaatcgc ttttgcgtac
    21541 atggtcggga atttttttcg cggatttggc acacgcttca ctgaatcggt gcggcaaatg
    21601 atgcttgcgc acggcaattt cgatttccat cccgctgtcg gcataatcgc ccaaaacttc
    21661 aatgattttt gccactgcag gccggttttg ctcgggataa acctcaattt tgccgacgat
    21721 aacctgaccg gattcgggtt tgaaacgcgc cacgccgtcc ggttccaaca cgatgctttg
    21781 gttcagacgc ttgtcttccg gctccaaaat cgccacgccc ctatccatat agaaacggcc
    21841 gacaactttg ctttgcgcgc gttcgacaat atccagaacc gtcccttcgc ggcggcccct
    21901 gcggtcgata cccgcaggac ggacggtaac ggtgtcgccg tgcatgacac cacgcatctg
    21961 gcgttcgtat aaaacgaaat ccccttcgtc catcggcatg agcggcacgg cgaagccgaa
    22021 accgtcctta tgcgcctcga cgcggcattt gaccaaatcc agcttgtccg ccgcgcaaac
    22081 tgcgcctcgg cggttgatta aaacctgacc gtcccgcgcc atcgccttca gacggcgttc
    22141 aaaaaagaca tactcgtctt ccgtaatcga cagctcgcgt gcaagcgatt cgatttttga
    22201 aggcacacct ttgcgctcca acaattcgat tatccattcc cgactgggca aaggatgttc
    22261 ataacgctgt ttttcacgac ttaaaaacgg gtctttttcc cgtaaattta aagatttaat
    22321 atttttattc attttgactg ttgacattct ctttatgaac tatataatag caacttcttt
    22381 gtggcaggac actgtaccgc aaaacagcaa ataaagcaaa gcccgggtgg cggaattggt
    22441 agacgcgcca gcttcaggtg ctggtatcct cactggtgtg gaagttcgag tcttctcccg
    22501 ggcaccaaat aacaaattat cccgattagg ataatttccg ccattagaga ggtggatgag
    22561 tggtttaagt cgcacgcctg gaaagcgtgt atacgtgaat agcgtatcga gggttcgaat
    22621 cccttcctct ctgccaaata caaatcccaa acttttacag tttgggattt tttgttactt
    22681 acgtattgat tttattgcgt aaaatcagct acaacctatt accctacgtt accattgatt
    22741 actatccgtt accgtttttt tatggtatgt tttgcggtat gttttatcct cttcctaagc
    22801 ctgtaaggac tcaaaaggcg tatcccttca aacaatattg ttcgacatgc tgccgggaaa
    22861 ccgaacccga taccgaaacg cgacggggcg atatgccgct ggaaaaatgg cggcagtttc
    22921 ttgattggat aggaaaaact ccgtctgaaa cagcttcaag aagccgcaaa gcccgtccga
    22981 ataaaaaata ccgtctgaaa tttttcagac ggcatcgcgt atcatttaaa cattaatcca
    23041 gctttttaaa atgacggcgg cgttccaatt cgctcaggta acgcttgcgc aggcggatgg
    23101 attgcggcgt gatttccacc agctcgtcat cgtcgataaa ctcgaccgcg ccttccagcg
    23161 tcagtttgat cggcgtggtc aggcgcaccg cttcgtcggt accgctggca cggatattgg
    23221 tgagtttttt gcctttgagc gggttgacca ccaaatcgtt gtcgcggctg tggatgccga
    23281 taatcatacc ttcgtagatt ttgtcgttgg gcgatacgaa catacggccg cggtcttcaa
    23341 gattccacaa ggcgtaagca accgcctcgc cctgctcttg ggacaccagt acgccgttgt
    23401 ggcggccggg catatcgggt ttgacgggcg cgtagtcgtc gaacacgtgg ctcatcagcc
    23461 cgacgccgcg cgtcagggtc atgaattcgc cttggaaacc gatcaagccg cgcgctggaa
    23521 tatggtattc gaggcgggtg cgtccgttgc cgtcgctttc catattggtc agttcgccac
    23581 ggcggcggcc gagttcttcc attaccgcgc cttggttgtc gtcgggtacg tccacagtca
    23641 ggttttcata aggttcgcat ttttgaccgt cgatgtctcg gtacacgacg cgcggcttgc
    23701 cgacggcgag ttcgtagcct tcgcggcgca tattttccag caaaatcgtc aggtgcagtt
    23761 cgccgcgccc ggatacgcgg aacacgtcgg catcggcggt gtcttccacg cgcagggcaa
    23821 cgttggtcag caattctttt tgcaggcggt cgcggatttg gcggctggtc acgaatttgc
    23881 cttctgtacc tgcgagcggg ctggtgttta ccataaagtc catcgtcagc gtcggttcgt
    23941 ccacgctcaa catcggcagg cctttggggt tgtctttgtc ggtgatggtt acgccgatgc
    24001 cgatgtcttc gataccggaa ataatcacga tgtcgccggc ttcggcttct tcaagcggca
    24061 cgcgttccaa gcctttgaaa cccaaaagct ggttgatgcg gccttgggcg atttgctgct
    24121 cgtggttcat cacggcaacg gtttggccgg gtttgatgcg tccgttcaag atacgaccga
    24181 taccgaggcg gccggtgtag ttgtcgtagt cgagttggga aatttgcagt tgcagcggct
    24241 cgtccgcgct gccgctcggt gcaggcgtgt attttaggat ggtgtcgaac agcgggcgca
    24301 tatcgctgct ctcgtcggtt tcttccagct tggcaaagcc gctcaaacct gaagcgtaaa
    24361 caatcgggaa atccaactgc tcgtcggtcg cacccaagtt gtcgaacaac tcgaaagtct
    24421 ggtcgataac ccagctcgga cgggcggacg gtttgtcgat tttgttgatg acgacaatcg
    24481 gtttcagccc caaagccaag gcttttttgg tcacgaaacg ggtttgcggc atcggacctt
    24541 cctgtgcgtc caccaacaag acgacgcaat ccaccatccc caaaacgcgc tccacttcgc
    24601 cgccgaagtc ggcgtgtccc ggcgtgtcga cgatattgat gtggcagcct tcgtaatcga
    24661 tggcggtgtt tttggcgagg atggtgatgc cgcgttcttt ttcaaggtcg ttgctgtcca
    24721 tcacgcgctc gtcaacctgc tggttggcgc ggaatgtgcc ggattggcgc agcagttggt
    24781 cgaccaatgt ggttttgccg tggtcgacgt gtgcgatgat ggcgatgttg cggatttgtt
    24841 tcataattaa tgtttttcaa aaactgtaag agataactgc gtattatacc acgatttacc
    24901 ggacagtatt tggaaaatga agatgccgtc tgaagccctt gcctttcaga cggcatcttc
    24961 ttacatactt tcagttatcc agttgcgcag ccaccttgcc gccggggcga tttcccctgc
    25021 cgacagtcca gccgcaatgc ctgccgattc ctttataaca tccgccgccg cgccgtgcag
    25081 ccacgcgccc gcgcaggcgg cttcaaaaac cggcacgccc tgtgcgagca gactgccgat
    25141 gatgccgccc aatacgtcgc cactgcccgc cgttgccaat cccgcattgc cgctttcgtt
    25201 gacatagatt tccgtatcgg acgcggcaac caatgttttg tgccccttta aaaccacggt
    25261 tgcaccgaga attgccccta tcttccttac tgccgccgtc cgatccgcct gaacctgtgc
    25321 aactgtcgtt ccaagcaggc gcgcggcttc ggcggggtgc ggcgttaaaa tcaggttttt
    25381 acacccgcgc gccagatttc gggtttcggc atcggttgat aatatgttca gcgcgtccgc
    25441 atccaaaacg acggcttcgt ctcgattccg cgccaacact gccgccaccg tttccactgc
    25501 cgccgcatct gtacccaagc cgcaacccgc cgtccaagcg tttatgcctt gacgcttggt
    25561 caaaccgtct gccgtgtcca acataatctc gggaaaaccg gcaatgacgg caaaaggcag
    25621 cgtatcctga ttgaaacctg cccgcacctt accgcagccg agatacattg ccgccgatgc
    25681 cgccaatacg ggcgcgccgc tcatccctgc cgatccgccg actacggcga gcgtgccgaa
    25741 agtccctttg tgcgaatctt ccgcacgggc tttgaaaaca tggggaaaac gcaatgccgt
    25801 ctgaagcatc cggcggcggc tttcgcctga aagatggaaa acgggaaaca tttcccctcc
    25861 aatcggttaa aattaaggct cgaaaacaaa ccgattctac cgccggaaac cgattatgaa
    25921 aaacaaaacc aaagtctggg acttccccac ccgccttttc cactggctgc ttgccgcatc
    25981 cctgcccttt atgtggtata gcgcaaaagc cggcggcgat atgctgcaat ggcacacgcg
    26041 cgtcgggctg ctcgtccttt tcctgctcgt attccgcctc tgctggggca tttggggcag
    26101 cgataccgcc tgtttctccc gtttcgtccg aggttgggca ggtatacgcg gctatctgaa
    26161 aaacggcatt cccgaacata tccagcccgg acacaacccc ttgggcgcac tgatggtcgt
    26221 tgcgcttttg gccgccgtct catttcaagt cggcacgggg ctttttgccg ccgatgaaaa
    26281 caccttcagc accaacggct acctcaacca tttggtttcc gaacatacgg gcagccttat
    26341 acggaaaatc cacctcaact ttttcaagct gctcgccgtt ttttccgcag tccacatcgc
    26401 cgccgtcgcc gcataccgca tattcaaaaa gaaaaacctc gtccgcccga tgataaccgg
    26461 cttcaaatac atcgaaggca aaacctcaat ccgctttgcc ggcaaagccg cgcttgccgc
    26521 cgcattatcg gttgccgcgc ttgccgcagc cgccatcctg ctcctgtcct gaaacccgac
    26581 atcatgccgt ctgaagccct tttctgcctt tctgacggca ttttccccac cgcacaaacg
    26641 gtctgaatgc ccgaaacggc ttcaggccgt ttttccgtcc aaaaccgtta tatagcggat
    26701 taacaaaaat caggacaagg cggcgggccg caggcagtac aaatggta
//
LOCUS       DAXDZG010000022        26537 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXDZG010000022 DAXDZG010000000
VERSION     DAXDZG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26537
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.022"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            78..176
                     /locus_tag="ACFB78_000824"
     CDS             78..176
                     /locus_tag="ACFB78_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC1268859.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(247..1632)
                     /locus_tag="ACFB78_000825"
     CDS             complement(247..1632)
                     /locus_tag="ACFB78_000825"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC1268860.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1731..4121)
                     /gene="gyrB"
                     /locus_tag="ACFB78_000826"
     CDS             complement(1731..4121)
                     /gene="gyrB"
                     /locus_tag="ACFB78_000826"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC1268861.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGTKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4387..5259
                     /locus_tag="ACFB78_000827"
     CDS             4387..5259
                     /locus_tag="ACFB78_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC1268862.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            5347..7383
                     /locus_tag="ACFB78_000828"
     CDS             5347..7383
                     /locus_tag="ACFB78_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC1268863.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLAGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSESDECR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(7964..9127)
                     /locus_tag="ACFB78_000829"
     CDS             complement(7964..9127)
                     /locus_tag="ACFB78_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC1268864.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(9349..9914)
                     /locus_tag="ACFB78_000830"
                     /pseudo
     CDS             complement(9349..9914)
                     /locus_tag="ACFB78_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            complement(10430..11944)
                     /gene="katA"
                     /locus_tag="ACFB78_000831"
     CDS             complement(10430..11944)
                     /gene="katA"
                     /locus_tag="ACFB78_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC1268865.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            12294..13144
                     /locus_tag="ACFB78_000832"
                     /pseudo
     CDS             12294..13144
                     /locus_tag="ACFB78_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            complement(13546..14673)
                     /locus_tag="ACFB78_000833"
     CDS             complement(13546..14673)
                     /locus_tag="ACFB78_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1268866.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGVNPFSDIKSTFLIFKALKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEVIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(14900..15022)
                     /locus_tag="ACFB78_000834"
     CDS             complement(14900..15022)
                     /locus_tag="ACFB78_000834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268867.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            complement(15027..16252)
                     /locus_tag="ACFB78_000835"
                     /pseudo
     CDS             complement(15027..16252)
                     /locus_tag="ACFB78_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16645..17892)
                     /gene="fabF"
                     /locus_tag="ACFB78_000836"
     CDS             complement(16645..17892)
                     /gene="fabF"
                     /locus_tag="ACFB78_000836"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC1268868.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(18048..18284)
                     /gene="acpP"
                     /locus_tag="ACFB78_000837"
     CDS             complement(18048..18284)
                     /gene="acpP"
                     /locus_tag="ACFB78_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC1268869.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            18516..19523
                     /locus_tag="ACFB78_000838"
     CDS             18516..19523
                     /locus_tag="ACFB78_000838"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC1268870.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            19720..20193
                     /locus_tag="ACFB78_000839"
     CDS             19720..20193
                     /locus_tag="ACFB78_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC1268871.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            20207..20542
                     /locus_tag="ACFB78_000840"
     CDS             20207..20542
                     /locus_tag="ACFB78_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268872.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            20736..23426
                     /gene="glnE"
                     /locus_tag="ACFB78_000841"
     CDS             20736..23426
                     /gene="glnE"
                     /locus_tag="ACFB78_000841"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC1268873.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPTGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFIKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            23850..26057
                     /gene="uvrD"
                     /locus_tag="ACFB78_000842"
     CDS             23850..26057
                     /gene="uvrD"
                     /locus_tag="ACFB78_000842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC1268874.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            complement(26315..>26537)
                     /locus_tag="ACFB78_000843"
     CDS             complement(26315..>26537)
                     /locus_tag="ACFB78_000843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268875.1"
                     /translation="WVALPLPEPRNGCLPWAGAGGGRAVCRYDTEVPIYKGGLQPAKS
                     TNSAESADSRRRHSRAGGNLVLSVRKLIG"
BASE COUNT         6068 a         7337 c         7202 g         5930 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaaattct tgccggatgc tgcaaacaac
       61 gccggtttgc attcctgatg gcggtggttt tcttggacga acgcccgaac acgcaggaat
      121 ggataggctt ggggctggtt acggcgggcg tgttgacgtt ggcgttgaag cggtaaaccc
      181 gcaaaaaata aatgaaatgc cgtctgaaaa actgttttca gacggcattt tcgtttctgt
      241 ccatcctcag cactcgacca cgcgcacgga tacggggacg gcttttttcc ggagcgtggc
      301 ggcgagtccg gcaagcgagg tttctttgta ggtcgccttc atgtcccggc cggtttgcag
      361 catggttcgg atgacttcgt cgagcgagac ttttttgtcc gtgccgtctt ccaagagcgc
      421 gagcgtgccg agtttgaggg ctttttcggc ggcgatgccg ttgcgttcga tgcaggggat
      481 ttgcaccaac ccgccgacgg ggtcgcaagt taagcccaaa tggtgttcca ttgccatttc
      541 ggcggcgttt tccacttgtt tgggcgtgcc gccgatgact tcggcgtatg cacccgccgc
      601 cattgaacac gctacgccga cttcgccctg acaaccgaca tccgcgccgg aaatggaggc
      661 gttggttttg tagaggatgc cgattgcgcc tgcggtgagc aggaagtttt cgacacgttc
      721 ctgtgtggcg tgggggttga acttgcggaa ataatgcaat acggcgggaa tgatgcctgc
      781 cgcgccgttg gtcggggcgg taacgacgcg tccgccggcg gcgttttctt cgttgaccgc
      841 catggcgtac accatcggcc agagctgggt gttgacgatt tcggtttcgc gcaggacttt
      901 gagtttggcg gcaagctgcg gggcgcggcg gcggacgttc aatccgccgg gcagttcgcc
      961 gtccgcaccc aagccgcgtt tgatgcagcc ttccataacc tcggcaacgg cagcgacgcg
     1021 gcggcggatt tcggcttcgc cgcatccggc aagcgcggct tcgtttgcca acacgacttc
     1081 ggagatgtcg agccggttca gacggcatcg ggcaagcagc ccggcacaac tgttgtaagg
     1141 ataaggaacg gctttttccg tttccgactg ccactcgaag tcttcttcgg taacgacaaa
     1201 gccgccgccg accgaataat aaacctgttc cttcaatacc gtgccgtctg aagcataggc
     1261 ggtaaatcgc aggccgttgg ggtgtttggg cagcacttga ttgccgcgta tgttcaggtc
     1321 gcggtcgggg atgaagcgga tttcttgccc gttgagccgg aggatgtgct gcgtgcggat
     1381 gcgtccgagg cgttcgggaa tgccggcaag cgggatgtcg tgcggcaggc tgccttccaa
     1441 accgagcatc agcgcgtcaa atgtgccgtg tccgtatccg gtcagggcaa gcgagccgta
     1501 aatgtcgatg gcgatgcggg cggtttgcgc gtccaagcct gcggcaaagg cggcggcggc
     1561 cttcatcggg ccgaccgtat gcgaactgga agggccgata ccgattttga aaatatcaaa
     1621 aatgctgatc atggttttgc tccgaaaggc ggtttcagac ggcatggctt ccgtttgaca
     1681 aaccaaaagg agacgcggca agctgcccgt ctcctttttt aaaacggcac ttatgcgtcg
     1741 atattttgcg caatcagcgc attgttttcg ataaaggcgc ggcgcggttc gacctcgtcg
     1801 cccatcaggg taacgaacac ttcgtcggcg gcaatggcat cttcgatgcg cactttcaac
     1861 aggcggcgca cggcgggatc catcgtggtt tcccaaagct gctcggggtt catctcgccc
     1921 aaacctttgt atcgttggat ggacatacct ttttgggcaa cgctcatcaa gatgtccaaa
     1981 gcggtttcaa agctgtccgc gtcgtactcg ttctcgcctt tgtagagctt ggtgccctcg
     2041 ccgaccaagc ctttgagcgc ggcggcggtt tgggtgaggg tttggtaggc tttgctgttg
     2101 aggaacttgg gttcgatgta gctgaccatg acgttgccgt gcagcttgcg cgtgattttg
     2161 atgaaccggt gtccttcatg accttcgatg cgttcgaggg cggcttcttt ttcgtcaagc
     2221 aaaccggaaa gttcggcaac ggctttatcg gcgttttcag acgacgtcaa atcaatgggc
     2281 gacgcgtgca gcatggcacg caggacgagt tcgtccacga agcggctttc ctgttcgatg
     2341 acggttttcg ccaacaagaa ttgtttggcg gtgtcggcaa gttctgcgcc ttcgatggtg
     2401 cggccgtctg aaacgatttt ggctttttcc aaggcaaggc cgagcagcca ttggtctttt
     2461 tccagttcgt ctttgaggta acgctcctgc ttgccgtatt tggctttgta gagcggcggc
     2521 tgggcgatgt aaatgtagcc gcgctcgacc agttcgggca tttggcggta gaagaaggtc
     2581 aggagcaggg tgcggatgtg cgcaccgtcc acgtcggcat cggtcatgat gatgatgcgg
     2641 tggtagcgta gtttttcagg gttgaactct tctttgccga tgcctgcacc cagcgcggta
     2701 atcagggtgg cgacctcttg gctggcgagc attttttcaa aacgtgcttt ttcgacgttc
     2761 aaaattttac ctttgagcgg caaaatcgct tggaatttgc ggtcgcggcc ctgcatggcg
     2821 gaaccgcctg cggagtcgcc ctcgacgagg tagagttcag acagggcagg gtctttttct
     2881 tggcagtcgg cgagtttgcc gggcagtccc aagccgtcca tcacgccttt gcggcgggtg
     2941 atttcgcggg ctttgcgggc ggcttcgcgt gcgcgggcgg catcgacgat tttgccggtg
     3001 atgattttgg cttcgttcgg attttcttcg aggaagtcgg ttagtgcttg gttgatgact
     3061 tcgttgacaa cggggccgat ttcgccggaa accagtttgt ctttggtttg ggatgagaat
     3121 ttggggtcgg gcagtttgac ggacaacacg caggtcaaac cttcgcgcat atcgtcgccg
     3181 gcggtttcca ctttggcttt tttggcgact tcgttagctt cgatgtagct gttgatggtg
     3241 cgcgtcatca cttggcgcag cgcggtcagg tgcgtaccgc cgtcgcgctg agggatgttg
     3301 ttggtgaagc actgcacgct ttcctgatag ctgtcgttcc attgcattgc gcattcgacg
     3361 ctcatgccgt ctttctcgcc gaacgcatag aagatttttt cgtgcaaggg cgtttttttg
     3421 cggttcatgt attgcacgaa gcccgccacg ccgccggaaa gggcgaagct ttcgtgcttg
     3481 ccgtcgcgct cgtcggtcaa ttcgatgtcc acgccgttgt ttaggaacga aagttcgcga
     3541 atacgtttgg cgaggatgtc gaagctgtat tcgatattgc cgaaggtttc cgtgccggcg
     3601 aggaagcgca cggtcgtgcc ttttttgtcg gaatcgccga caattttcag cggctcttcg
     3661 gtttcgccgc gtacgaagcg gacgaagtgt tctttgccgt cgcggtagat ggtcagcgtt
     3721 acccagtcgg acagcgcgtt gacgacggat acgcccacgc cgtgcaggcc gccggagatt
     3781 ttgtagctgt tgttgtcgaa tttgccgccc gcgtgcaaga cggtcatgat gacttcggcg
     3841 gcggaacgcc cttctttcgg gtggatgccg gtgggcatac cgcgcccgtt gtcggcgacg
     3901 ctgacggaat ggtcggcgtg tatcgttacc gtgattttgt cgcaatgtcc ggcgagtgct
     3961 tcgtcgatgg cgttgtccaa tacttcaaac accatatggt gcagcccgct gccgtcctgc
     4021 gtgtcgccga tgtacatgcc ggggcgtttg cgtaccgctt ccaagccttc gagcacctgg
     4081 atgctgtcgg cgccgtattc ttcgtgtttt tgttcagtca tattttttgc cggattttga
     4141 aaagattttg cgatgccgcc aaaacagtcc gcaccttgtg gaaaaagcgg gcgggacggc
     4201 gcatataatt gtgtattata gccgatttcg ccgcctgatt cagcgttatc cgcatccgga
     4261 tgccgtctga aagggcgtgc ggcgcatttg cggccggtac gggcgcggaa tttgccggca
     4321 aagtggcaaa agcgtttttt tgccactaaa atctacaccc tatacttttc ggacaggggc
     4381 gcggaaatgg aaatatggaa tatgttgaac acttggcccg atgccgtccc gatacgcgcg
     4441 gaggcggccg aatccgtggc ggcggtcgcg gctttgctgc tggcgcgcgc ccttctgttg
     4501 aatatccact tcagacggca tccggatttc ggcatcgaaa gcaagcggcg gtttttggtt
     4561 gccagccgca atataacgct gcttttggtg ctgttttcgc tggcatttat ctggtcggcg
     4621 caaattcaaa cgctggcgtt gtcgatgttt gcggtggcgg cggcggtcgt cgtggcgaca
     4681 aaagaactga ttatgtgtct gtcgggcagt attttaaggt ctgccaccca gcaatactcg
     4741 gtcggcgact atatcgaaat caacggcctg cgcgggcgcg tggtcgacat caatctgttg
     4801 aacacgctga tgatgcaggt cggtccgaac cccttggtcg gacagcttgc gggaaccacc
     4861 gtttctttcc ccaacagcct gttgttgagc caccccgtgc gccgcgacaa tattttgggc
     4921 gactatgtca tccatacggt cgaaatcccc gttcccatcc atttggattc ggatgaagcc
     4981 gtatgccgtc tgaaagccgt actcgagccc ttgtgcgcgc cctacatccc cgccattcag
     5041 cggtatttgg aaaacgtgca ggcggaaaaa ctgtttatca cgcccgccgc caggccgcgc
     5101 gttacccgcg taccgtacga cgacaaggca taccgcatca tcgtccgctt cgcctccccc
     5161 gtttcaaagc ggctggaaat ccaacaggcg gttatggacg aatttttgcg cgtacaatac
     5221 cgcctgttaa atcatcccgc cggctccgaa acactttaac tttccccggc cggccccatt
     5281 tctggcttca gacggcatat tgccgatatg ccgtctgaag cacaacacgc aaaggaaacc
     5341 catcttatga ttgacaacgc actgctccac ttgggcgaag aaccccgttt taatcaaatc
     5401 aaaaccgaag acatcaaacc cgccgtccaa accgccatcg ccgaagcgcg cggacaaatc
     5461 gccgccgtca aagcgcaaac gcacaccggc tgggcgaaca ccgtcgagcg tctgaccggc
     5521 atcaccgaac gcgtcggcag gatttggggc gtcgtgtccc atctcaactc cgtcgtcgac
     5581 acgcccgaac tgcgcgccgt ctataacgaa ctgatgcctg aaatcaccgt cttcttcacc
     5641 gaaatcggac aagacatcga actgtacaac cgcttcaaaa ccatcaaaaa ttcccccgaa
     5701 tttgcaacgc tttcccccgc acaaaaaacc aagctcgatc acgacctgcg cgatttcgta
     5761 ttgagcggcg cggaactgcc gcccgaacgg caggcagaac tggcaaaact gcaaaccgaa
     5821 ggcgcgcaac tttccgccaa attctcccaa aacgtcctag acgcgaccga cgcgttcggc
     5881 atttactttg acgatgccgc accgcttgcc ggcattcccg aagacgcgct cgcgatgttt
     5941 gccgccgccg cgcaaagcga aggcaaaaca ggttacaaaa tcggcttgca gattccgcac
     6001 taccttgccg ttatccaata cgccggcaac cgcgaactgc gcgaacaaat ctaccgcgcc
     6061 tacgttaccc gtgccagcga actttcaaac gacggcaaat tcgacaacac cgccaacatc
     6121 gaccgcacgc tcgaaaacgc attgaaaacc gccaaactgc tcggctttaa aaattacgcc
     6181 gaattgtcgc tggcaaccaa aatggcggac acgcccgaac aggttttaaa cttcctgcac
     6241 gacctcgccc gccgcgccaa accctacgcc gaaaaagacc tcgccgaagt caaagccttc
     6301 gcccgcgaac acctcggtct cgccgacccg cagccgtggg acttgagcta cgccggcgaa
     6361 aaactgcgcg aagccaaata cgcattcagc gaaaccgaag tcaaaaaata cttccccgtc
     6421 ggcaaagttc tggcaggcct gttcgcccaa atcaaaaaac tctacggcat cggattcgcc
     6481 gaaaaaaccg ttcccgtctg gcacaaagac gtgcgctatt ttgaattgca acaaaacggc
     6541 gaaaccatcg gcggcgttta tatggatttg tacgcacgcg aaggcaaacg cggcggcgcg
     6601 tggatgaacg actacaaagg ccgccgccgc tttgccgacg gcacgctgca actgcccacc
     6661 gcctacctcg tctgcaactt cgccccgccc gtcggcggca aagaagcgcg tttaagccac
     6721 gacgaaatcc tcaccctctt ccacgaaacc ggccacggac tgcaccacct gcttacccaa
     6781 gtggacgaac tgggcgtgtc cggcatcaac ggcgtcgaat gggacgcagt cgagctgccc
     6841 agccagttta tggaaaactt cgtctgggaa tacgacgtct tggcacaaat gtccgcccac
     6901 gaagaaaccg gcgagcccct gccgaaagaa ctcttcgaca aaatgctcgc cgccaaaaac
     6961 ttccagcgcg gtatgttcct cgtccggcaa atggagttcg ccctcttcga tatgatgatt
     7021 tacagtgaaa gcgacgaatg ccgtctgaaa aactggcagc aggttttaga cagcgtgcgc
     7081 aaagaagtcg ccgtcatcca accgcccgaa tacaaccgct tcgccaacag cttcggccac
     7141 atcttcgccg gcggctattc cgcaggctat tacagctacg catgggccga agtcctcagc
     7201 gccgacgcct acgccgcctt tgaagaaagc gacgacgtcg ccgccacagg caaacgcttc
     7261 tggcaagaaa tcctcgccgt cggcggctcc cgcagcgcgg cggaatcctt caaagccttc
     7321 cgcggacgcg aaccgagcat agacgcactg ctgcgccaca gcggcttcga caacgcggct
     7381 tgatagtcag gttgatataa aaagcggatt cgatgtagaa atacctgaat cgttatcccc
     7441 gcgcaggcgg gaatccggaa cataaaattg aagaaaccgt tttacccgat aagtttctgt
     7501 gccgacaagt ctggattccc gcctgcgcgg gaatgacgac aaagatattt ttatttgaaa
     7561 tttactataa aaggccgtct gaaaattttt cagacggcct ttttataatc taaaactgtc
     7621 ggattcggga atacaatcca aacaggctat tgattatcat aatgccgaag tacaaacccc
     7681 caaagccgat ggtaatggta gcgcaagaat attgattaat tatccaaacg catcatggct
     7741 tacacaacgc agccattgaa ttaggcttca gcccaccaat ccctccgcct acgccgcgca
     7801 ttcgtgaaaa acgcataaga cccggacatg gttggttgaa ttggtggaaa cggctggctg
     7861 aagcacaccc tacggcatac cctgcgtatc tgacgcgacg aaatataaaa tgccgtctga
     7921 aacagattcc cgtttcagac ggcatttttg cgtaccggcc ggattatttg ttgtccggct
     7981 tagattccat cggtgcggtc aggggcagtt ccggcatcgt gcgcgctgat tcggaaacat
     8041 tgccggaaag tgcattcagg aatacgacga tgttatccac atcttctttc ggaatgtctt
     8101 tacccaattg cgccttaccc atgatggtaa ctgccttatc cagctcccac acgctgccgt
     8161 tgtggaaata cggataagtt ttagccacgt tacgcaaccc cggaacacgg aagaaaaatt
     8221 cgtcttcggt ttttttggtt acgtcagcac ggcctttgtc gcgtttcgga tcttcaatga
     8281 atttccaata cggcccttgg accagaccga atttctggaa ggtcgtgcct ccaaggttga
     8341 caccgttgtg gcaggcaata cagccgttgt ccatgaacgc gcgcacgcct tttcgttcct
     8401 gttcgctcag ggcgttgacg ttgcctttga ggtattcgtc ccatttggtc ggcgtcagca
     8461 gggtacgctc aaacgcaccc aatgcggtag tgatgttttt aaacgaaacc gcgccgtctt
     8521 caggaaaagc ttttttaaac atttcttgat attcgggaac tttggcgatt ttggctgcag
     8581 ccgcctcttg cgaatcattc gccatttcca ccggattcac caaaggcccg ccagcctgtt
     8641 cttcaacatc ggcggcacgt ccgtcccaaa actggctgcc cagcagcgca gcattcaatg
     8701 cggtaggcga gttgcgtccg ccgaactgcc ctttgtgccc ctgactggtc ggcatattgt
     8761 ccacaccggc ggaagcaagg ttgtggcaag agttgcagct tacggtattg cctttggaaa
     8821 ggcgtggttc ataccagagt tggtgtccga gtttgacctg ttcttcggta aacggacgga
     8881 ttttctgcat ttcttcgaca gtcggcaaag gttggaatac accttgcgcg cgtttcaaaa
     8941 ggtcttggtc ttcgggcgag gccgatgaag cggaaggaac ggcttccgag gcctgtactt
     9001 gggaagccgc ttcggtctcg gaagcaggag aagcatcgcc tgccgcagac ttttcctgac
     9061 cgccgcatgc cgccaacagt gaagacaatg ccaatactga tgcgaggtaa cggagtttga
     9121 aagacatcgt gtattcctta tgggaggtta aaatcattgt tcaaaccggc aggcaaacct
     9181 gttctgccga ttatttcttc agtgtaccta tcgcaccttt agccatcttt gcggaaaatc
     9241 aatattttat tttcaatagc tttacacaat aataccgata gagaatcaag tttaacaaaa
     9301 tgccgtctga aaccggtaaa gcttcagacg gcattttatt ttccgacatc attgaatcaa
     9361 acccaaatgc gataagagcg tccatgtacc gatggcaatc aacaccaaac ctccggcaaa
     9421 ttccgcacgc ctgccgaaca atacgccaaa agcctttccc gccgtcagcc cgatggtaac
     9481 cattaccgta gccgccatac cgattacggc ggcggcaaag gcgatgttta cctccataaa
     9541 cgccaagccc accccgacga tcatggaatc aatactggtt ccaaaagcag tcaaaaccgt
     9601 catccatagg ctttcctgtt tgctttcgcg cacatcttcc gcctcgccgg acaacccttc
     9661 gcgcatcatt ttcagaccca gtccgcccaa caggacaaaa gccacccaat ggtctcattc
     9721 gctgataaac ggcttggcat aaaaaccgcc tacccagccc gccagcggca tgaacgcttc
     9781 aaccgtaccg aacaccaaag ccgttgctgc aattttgcgc ggggcatcct gaccgccgca
     9841 ccctttgcca gcgcgacggc aaacgcatcc atcgacattc ccagcgcaac caagagcaaa
     9901 gcgtaaagac tcataccgca cccatcctta aaaagggcgg attataacaa aacagcaaag
     9961 cgcgaaaaac gccgcacgaa aacccgcatc ccgttattcc cgcaaaaaca aaaaatcaaa
    10021 aacagaaatc ccgtcattcc cacgaaagtg ggaatccagc cccgtcggca cggaaactta
    10081 tcggataaaa cggtttcttt agattttacg ttccggattc ccactttcgc gggaatgacg
    10141 cggaaaagtt ttcgtgccgg tttgaaccgg tggggatggt gcgggaatga cgggatattt
    10201 tgcgtttata gtggattaac aaaaatcagg acaaggcgag gcaacgccgt accggtttaa
    10261 atttaatcca ctataataaa aaacgcccac tgaaacggcg gggcgaggtg tggaaatgcc
    10321 gtctgaaacg gtcaaacgac gtttcagacg gcatttttat gcccggttat ttccggtatc
    10381 ggacggcgcg ggacaggatt tcttcaattt ccatccacat aatgccccct tacagcaaac
    10441 cggcctgacc cagtgcggga tcggtcgcgc gggcggcttg tgcgtcttcg acggtcagtc
    10501 cgagggcttt ggctacgcct tcaccgtatg ccgggtcgca acggtagcag ttgcggatat
    10561 ggcggtattt gatgaagtcg ggcgcatcgc ccatggctgc ggcggtgttg tcaaacaaag
    10621 cctgtttctg cgcgtcgttc atcaggttga acaaggcgcg cggttggctg aagtagtcgt
    10681 catcgtcttg gcggtagtcc cagtgtgccg cgtcgccgtt gattttcaaa ggcggttcgg
    10741 cgaagtcggg ttgttgctgc cattggccga agctgttggg ttcgtagtgc ggcaggctgc
    10801 cgtagttgcc gtcggcgcgg ccttgtccgt cgcgctggtt gctgtgaaca gggcaacgcg
    10861 gacgattgac ggggatttgg cggaagttca cacccaagcg gtaacgttgc gcgtcggcgt
    10921 aattgaacaa acgggcttgc aacattttat ccgggctcgc gccgataccg ggaacgaggt
    10981 tgctcggtgc gaaggcggat tgttccacat cggcgaagaa gttttcggga ttgcggttca
    11041 actcgaattc gcccacttca atcagcggat agtctttttt cggccaaact ttggtcaagt
    11101 caaacggatg ataaggcact ttttcggcat cggcttcagg catgacttgg atgtacatcg
    11161 tccatttcgg gaactcgccg cgctcgatgg cttcgtacag gtcgcgctga tggctttcgc
    11221 ggtcgtcggc gatgattttt gcagcttctt cgttggtcag gtttttaatc ccttgctggc
    11281 tgcggaaatg gaatttcacc caaaaacgtt cgcccgcttc gttccagaag ctgtaggtat
    11341 gcgaaccgaa gccgtgcata tggcggtagc tggcgggaat accgcggtcg ctcatcacga
    11401 tggtaacttg gtgcagggct tcgggcagca gcgtccagaa gtcccagttg tttgtggcgg
    11461 aacgcatatt ggtgcgcgga tcgcgtttga cggctttgtt caggtcgggg aatttgcgcg
    11521 ggtcgcgcag gaagaacacg ggcgtgttgt tgccgaccac atcccagttg ccttcttcgg
    11581 tatagaattt caacgcaaaa ccgcggatgt cgcgttccgc atcggctgcg ccgcgctcgc
    11641 ctgccacggt ggtgaaacgg gcgaacatct cggttttttt gccgacttcg ctgaaaattt
    11701 tggcgcgggt gtatttggtg atgtcgtgtg ttacggtaaa cgtaccgaac gcgcccgaac
    11761 ctttggcgtg catacggcgt tcggggatga cttcgcgcac gaagtcggcg agtttttcat
    11821 tcagccacaa atcttgcgtc agcagggggc cgcgcgggcc ggcggtcagg ctgttttgat
    11881 tgtcggcaac gggcgcgccg ttgttcatgg tcagatgggt tacggggcat ttggaggtag
    11941 tcatcgctct tgttcctttt ctcaggttgg tcaaatgggg ggcaaacggc ttacagtacg
    12001 atttggcgga aagcgtattc gtaaccggtt tcttgattgt aataaatttc ttgaatcgac
    12061 attttatttt ccttttgcaa aaactatgga tgcgattata cgccaagatt ttcgttatta
    12121 aaactatgaa attgatttaa tattgttata agcaatcaag tttccatttt cgtttgtttt
    12181 ttgttatcgg acggaatccg aacccgctca ttaaaaccat ttataatgca atgccgcttt
    12241 gcggcatttt tttgcgccga caggctgaaa ataacaattt tcaccgcatt atcattacct
    12301 taatcggaat aaagctcaaa cagacccagc agctcgacca gcggctgcaa caatctttgc
    12361 gcgtattgca gatgccgggt atcgaacttg aacgcgaggt cgaaaactga ccgtcggaca
    12421 accccctgct cgaacgcaaa gaaacggatg aattttccga tgccgaattc agccattaca
    12481 ccgcgcccgc gcgtcaaatc ggcggagacg aaggcgaaga tatgctgtcc aacatcgccg
    12541 gcgaagaaga tttcaagcaa tacctgcacg cgcaagcgtg cgaacacccc ctttccgacc
    12601 aagaagccgc ctgcgtccac atccttatcg atttccttga cgagcagggc tacctgaccg
    12661 acagcatcga agacatcctg gaccatacgc ccttggagtg gatgttggat gaagcaatgc
    12721 tgaaacaagc cctgaccgca ttgaaaaaat tcgatccggc aggcatggcc gccgccgatg
    12781 tgaccgaatc gttgatattg cagatagaaa gatcgggcga atgtgctgcc aaacccgccg
    12841 ccctgcatat cgtccgaaac gccctcgaca gcattgacgg caaccgcagc caaaccccgg
    12901 cgcgaataaa aaacgcctgc cccaaaccga cagcggcaca ctcgaagccg cactcggcct
    12961 cattgcttcg ctcaacccct ttcccgccgc cggttttgcc tcgtccacgc ccaagtccta
    13021 ttctgacgag gcactcgcca acctgctggc tttccgcggt atagaggttt cccgccgcac
    13081 cgttgccaaa tacagagaat cccttgagat tccggcagca cacaaacgca aaaccgcaga
    13141 ataattaccg aataatctta taaagacaac aaaccaaaag ccgacatttc tgcgaaagcg
    13201 ggaatgccga atccgtccgc gcggaaacct gcatcccgtc attcccgcga aagtgggaat
    13261 ctagaacgta aaatctgaag aaactttttt tcgataagtt tctgtaccgg cggggctgga
    13321 ttcccgcctg cgcgagaatg acgggatata agttgctgtg ccggtttgag ccggtgaggt
    13381 tggtggcggg attggtttcg gtgggctgaa gcccacccta cagcccgccc tacacatctg
    13441 aaactcaacg aacctggatt cccgctttcg cgggaatgac ggggtttcgc gggaatggcg
    13501 ggagtttgtc agaaatcacc gaaactcaaa agccgaccac cttgtttacg ccttcaaaat
    13561 atcgagaaat ttcaaatcga ctttttcggc atcgaattta tctttggcaa tcgcataact
    13621 tgcattcccc atcaggcgga cggcagccct gttttcgata aaataaatca ttttttcggc
    13681 caagatgcgg ggattccaag gttcgatcag gaagccgttg accttgtcgg caaccgtttc
    13741 cctgcatccg gggacatccg tcgtaatcac cgccctgccg acggccattg cctcctgggt
    13801 gcttcgggga acgccttccc tataataaga cggcaatacg aatatatgat gttcttttat
    13861 cacttcggaa acattgttta caaaaccggg gaaacggata atatcgcggg cggcaggctg
    13921 ttccaaatcc ccgctcccgc gtggtttgtc gattgcgccc aaagcggtaa aaaccgtatc
    13981 ggggtatttg cccttaacct gttccgccgc ccgaataaaa tcatcaatcc ccttttcttt
    14041 cagaaatctg ccgataaaga ggaattttac gggttctttt tcatcgggaa tatccgcctc
    14101 ggaataagga tattgccgca aatccagacc gattccgccc aaaatatgga tgttttttat
    14161 tttgatgccg tattgatgca gcaactcgtc tttgtcgtcg gggtttaata caatcaggct
    14221 ttccaacatc ggcagggtaa tgcggtacaa ggcgattaaa atccccttta tgatttttgt
    14281 ttttaacggt atgccttccg gctgcggggt aaatgcgaat cccaaacctt ccagcatccc
    14341 gacgattctg ggcacgcctg ccaattttgc ggcaaaagtg ccgaaaatca cgggttttgc
    14401 gaaataaggg aaaaccaaat ccggcgatat ttttttgagt gctttaaata tgaggaaggt
    14461 ggattttata tcggaaaacg ggtttacccc gctgcggttt gaccggtagg taacgggtgt
    14521 aacccccatt tccctgataa tgtccaactc attgtcggaa aactccgata caaaggcata
    14581 cacctgatgg tttttgccga ttaatttttt aatgacgggg gcgcggaaac cgtaaatgct
    14641 ggatgcgact gttgtgataa aaacgatttt cataaggcgg acaccttgaa tataggattg
    14701 gaaatgcggt ctgcattgat gaaatgccgg atacggggcg cggcgggcaa acctttgcca
    14761 agcgcgccgc atccgacgct tcgcccgccc gggcaggggc ggcttcagac ggcattttat
    14821 cctgaaatcc gctgcataac gcccagcgat atacagtatc gcgttttgtg atgattttac
    14881 acccgaaggg aaaacgggct tatttgaatg gcggtttaat cgcggcggtt atttgcggtt
    14941 tgataaggct gatttgcgtg ccgtgcagtc cggcaacggg cggggtgttt ggaaaatgcc
    15001 gtctgaaggt ttcagacggc attctgtcat gcggcggttc ggggttcgcg gacgggcagg
    15061 acaagcaggg ctgccgtgcc ggcgagtacg gcatcggcgt accacatcca gccgtagtca
    15121 ccgaattgtg taatcacgat gccgccgatg tacgagccga ggaatccgcc gatttggtgg
    15181 gtgagcatca ccagtccgaa cagggtggcg aggtagcgcg tgccgaagag tttgccggta
    15241 acggcggcgg tcggggcgac ggtggcgagc catgtgaatc cgagtgcggc ggcgaaaatg
    15301 taaaagttga ggtcggtttt gggtgagaag atgtaaatca gcaccatggc gacgcgtgag
    15361 gcgtagagtc cgaacaggat gtgttttcct ttgaagcgtc cggtgcatca gcctgagaac
    15421 acgcaaccgg cgatgtttgc cagtccgatg atggcgatgg atgtcgaggc gacggtggcg
    15481 ggcagtccgc acagggcgac ttccgtgggt aggtgggtta cgagaaaggc gatgtggaag
    15541 ccgcaggcga aaaagctcag gtgcagcagg atgtagcttg gggttttgaa ggcggttttg
    15601 actgcttctc cgaggctttg tccgtgtgtg gcttgggtgt gttgggtgtg ggcggcgttg
    15661 ttgccgccgg caagccacca tgagacgggc agaatcagca gggcgattgc gccccaaacg
    15721 taaaatgtac ccgtccagcc gacttcgggc aggacgacga gtccttgagt ccttgaacca
    15781 gcggtgcgaa caggaattgt cctgccgaac cgcctgcgtt gaccagtccg gatgccaagc
    15841 cgcgtttgtg tgtggggact tgggcggcga cctgtcccat aatgatggaa aacccgccgg
    15901 aaccggtgcc gaatgcgagc agcagcccga cggcaatcat cagcccccaa taagtcggga
    15961 tgttggaggc aatcaggcag gcgcagacga gcaggagggc accgccgctt aataccctga
    16021 acgcgccgaa acggtcggca agcgcgccgg acaatggttg caatacgccc cacatcagtt
    16081 ggaaaacggc gatgatgagg ctgaactggg caatgttcaa ttcggtggtg ttgacgacgg
    16141 gttggacgaa cagtccgagc gtcatcctca tgccgatggt aatcagcagg atgaatgcgg
    16201 cggcggcaat gaccgcccag agtttgggtg ttttcgatgc ggtgtgcgtc ataatatgta
    16261 ttttgagtcg gttaagtttg cgaattttaa caaaccggat gattcgggct aaataattcc
    16321 tgattctttg gatatagcca acggtatgtc tgccgatgcc gtctgaaagg gcttgatggg
    16381 cgtttgcggt tcgggttaat atttgtgccg tatttgttaa ttccagttag tatagtggat
    16441 taactttaaa ccggtacggc gttgccccgc cttagctcaa agagaacgat tctctaaggt
    16501 gctgaagcac cgggcgaacc ggttccgtac catttgtacc gtctgcggcc cgccgccttg
    16561 tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acggtttcag acggcatttc
    16621 gatgtcggcg gcggctttgc ggaatcagcc tttgaagcgt ttgaagacca gcgtaccgtt
    16681 ggtgccgccg aagccgaagg agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc
    16741 gttggcgcag tagtccaaat cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt
    16801 cggcgggatt ttgccgtcgt gtatcgccaa aatgctgtac acggcctcca cgccgcccgc
    16861 cgcgccgagc aggtggccgg tcatggattt ggtcgagctg atgacggttt tgcaggcgtg
    16921 ttcgccgaac gcgcgtttga gggctttggt ttcgttggca tcgcccaagg gggtggacgt
    16981 gccgtgcgcg ttgacgtaat ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg
    17041 ggtaacggca agggcggggc cttcttcgtt cggcgcggtg atatggtaag catcggaact
    17101 catgccgaag ccgacgattt cggcgtagat tttcgcgccg cgttttttgg cgtgttctaa
    17161 ttcttccaac accaatatgc ccgcgccttc gccgatgacg aagccgtcgc gacctttgtc
    17221 ccacggacgg gaagcggtgg cggggtcgtc gttgcgggtg gagagggctt tcatcgcagc
    17281 aaaaccgccc acgcccaagg tgctgattgc gccttccgcg ccgccggcaa ccattatgtc
    17341 cgcgtcgccg tatttgatca gtcgggcgga atcgccgatg gcgtgcgcgc cggtggtaca
    17401 ggcggaaacc atgccgtagc tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga
    17461 aatcaggttg atcagcgaac cggggataaa gaaagggttg attttgcgcg cgccgccttc
    17521 gattacggct ttgccggtgg cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt
    17581 cacgccgatg cggtctttgt cgaggttttc cacatcgtcc aaacccgaat cggcaatcgc
    17641 ctgcaatgcg gcggcgatgc cgtagtggat gaacacgtcc atccggcgcg cttctttcgc
    17701 gctgatgtat tgtccgatgt cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc
    17761 ggatgcgtca aagcgggtaa tcgcgccgat gccgcttttg ccggcgagca ggttgtccca
    17821 agcctctgcg gcagtgttgc cgacagggga aacctgacct aagcctgtaa tgactactct
    17881 tctctgactc atgataacct cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt
    17941 gcagatgccg tctgtaattt gcggcagggg ttcaaacagt ttgccatata agggaaaagc
    18001 ctctattgcg cggtgcagca gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg
    18061 gcattgatgt agtcgatagc cagttggacg gtggtgattt tttcggcatc ttcgtcgggg
    18121 atttcgcagc cgaaggcttc ttccaaagcc ataaccagct ccacggtatc caaagaatcc
    18181 gcgcccaagt cgtcttggaa ggaagattcg tttttcacgt cggcttcgtt tacgcccagt
    18241 tgttcagcaa taattttctt aacttgttgt tcgatgtttg acatatcagt cgttccttta
    18301 tgccttgcgg caggttgttt aagggaaata aatcggtggt attgtaccga cttttaatag
    18361 agttttctat ctaatgccta ttatatcaat atttgcggat ttgtacattt ttgggtgcgg
    18421 cgggttttgt cgttcaagtt tgacctgtgt gccgtatgtt tggcgggatt tcggttaaaa
    18481 tggcggcatt tccatctgaa gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc
    18541 tgtttgcact cgatgccgaa aaagcccacc acttcacgct cgacgcgctc aacacggttt
    18601 ataaattggg cctgattccc gtaaccgaca accgtaccaa acctataaaa ctgatgggta
    18661 tggatttgcc caaccctgtc gggcttgccg caggtctcga caaaaacggt gaatacatcg
    18721 acgctttggg cgcgctcggt ttcggcttcc tcgaaatcgg cacggttacg cgcaacccgc
    18781 agcccggcaa cccgcagccg cgcctcttcc gcgttcccga acaccaaggc atcatcaacc
    18841 gaatgggttt taacaaccac ggcatcgacg cgatgatacg caatattgaa aaaagcaaat
    18901 atcaaggcgt attgggcatc aacatcggca aaaacgcggt tacgcccatc caaaacgccg
    18961 ccgatgatta tttaatctgc ctcgaaaaag cctacgcgca cgcaagctac attaccgtca
    19021 acatttcctc gcccaacacc aaaaacctcc gcgcgctgca aggcggcggc gaattgggcg
    19081 cattgctcga agccctgaaa aacaaacagg cgcagcttgc cgccgcccac ggcaaataca
    19141 tcccgctagc cgtcaaaatc gcccccgatt tggatgaagc acaaatcgaa gacatcgccc
    19201 gcgtcgtcaa atccgtcgaa atggacggca tcatcgctac caacaccacc atcgacaaat
    19261 caagcctcgg cagccatccg ctcgcaggcg agcagggcgg tttgagcggt tttcccgtgc
    19321 gcgaaaaaag caaccgggtg ctgaaaaaac tggcggaaca catagacggc gcattgccga
    19381 ttatcggcgt aggcggcatt atggaaggcg gggacgcggc agagaaaatc cgcttgggca
    19441 cgaccgccgt ccaagtgtac agcggattga tatacaaagg cccggcgttg gtcaaagaat
    19501 gtttgaaggc attggcgcga tgacgcgccc cacccaaaat gccgtctgaa cgcacgtttt
    19561 gccgttcaga cggcattttc atagattggt atccgactgt tgaagaggca atcagccgtc
    19621 atttggaaga cttttcgtaa caggcgtcaa tcgaaagggg cggaatgacg gggcaggcaa
    19681 accgatgcgt gaacacgggg caggcaaccg gagtaaaaaa tgaaccttga tttaaccgcg
    19741 caaaaagtcc gcctttcttg gaaggatatt ctgtgggggt atgagaataa atacttgggt
    19801 tgggctgatg tggcagctta tgcccgaaaa atgacgcttt cagatcatga tgaacgcgtg
    19861 ttcaagctat ctttaaccaa caaatccaat atttttgaat taaagcctgt tctggaagat
    19921 ttggcttcgg aaacgagggg ttattcccct aaaaattggc tgtacatcct cctaaatgac
    19981 gtattccata gaaaagaaga atttgaggat cctctggggg aagttgaaaa aatttatgcg
    20041 gattttgatt acccggaaga aatagaatca tttgtcaggt atatgccgcc caaagacggc
    20101 tatatccctt ctaatcatag ttatgaagaa aatattgccc ggttatattc tcactgggaa
    20161 cactatttga acaacggcgg cgggcagggt taaaaccggc aatccgatgc cgtctgaagc
    20221 gttatccggc gttcagacgg cattttgttt tccgacagtt tataaactgt cgttgtttct
    20281 tgacagaaac aacgacctta tttatttgaa acgattggag gacatgatta tgggtttttg
    20341 gggtagtgtg ggaaaagcag caaaagcagt aggcgaggga ttgattgaag ccggcaatca
    20401 gcataaggcg ttgaaaatgg aatatgcgga gaaatcaagt gaggagctgc atgaaatcgt
    20461 aaagagtgat ggttttttta aaaattccac acgggagaaa ggtgcggctt atgctgtttt
    20521 aaaagagcgt ggcgaggttt gaacgggaaa cggcggcatt tgccgctgtt tttttatcgg
    20581 caggcatccg tccgaatatc ggggcaaggt ttcagacggc atcgaaggtt gctatgatat
    20641 agtggcttga ctttaaaccg gtacggctcc gccttgccct gatttgaagt tcatccgcta
    20701 tcacattccc gccatccttt caaacggaat ccgaaatgtc cgaccaccgc ctcgacaccg
    20761 cccgccgcca ttccctgttc ctcgcccgcc agctcgacaa cggcaaactc aagcccgaaa
    20821 ttttcctgcc catgttggac aaggcgttga cggatgagga tttccaagcc tttgccgact
    20881 gggacaaaat ccgcgcggaa gaaagcgagg aagaattggc gcggcagttg cgcgagttgc
    20941 gccgttatgt ggtgtcgcag attatcgtgc gcgatataaa ccgcatcagc gatttgaacg
    21001 aagttacccg cacgattacg ctgtttgccg attttgccgt caataccgcg ctggatttcg
    21061 cctacgccta ttatcgggat atgtacggca cgccgaccgg gcgttatacc aaatcgccgc
    21121 agcatttgag cgtggtggcg atgggcaagg cgggcggcta tgagctgaac gtgtcttccg
    21181 acatcgattt gattttcgtc tatcccgaat cgggcgacac cgacggcagg cgcgaacggg
    21241 ggaatcagga gttttttatc aaagtcgggc agaaactgat tgcattgttg aacggcatta
    21301 ctgccgacgg gcaggtgttc cgcgtcgata tgcggctgcg gccggacggt gattcgggcg
    21361 cgctggtgtt gagcgaaacc gcgctggagc aatatctgat tacgcagggg cgcgaatggg
    21421 aacgctatgc gtggtgcaaa ggccgcgtag tcacgccgta tccgaacgac atcaaatcgc
    21481 tggtgcgccc cttcgtgttc cgcaaatatc tggattacgg cgcgtatgag gcgatgcgca
    21541 acctgcaccg ccaaatccgc agcgaagtca gcaaaaaagg catggcggac aacatcaaac
    21601 tcggcgcggg cggcatccgc gaggttgaat ttatcgccca gattttccag atgatacgcg
    21661 gcggccaaat gcgcgcgctg caactgaaag gcacgcagga aacgctgaag aaaattgccg
    21721 agatgggcat tatgccgtct gaaaacgtcg aaaccctgct tgccgcctac cgcttcctgc
    21781 gcgatgtcga acaccgcctg caatactggg acgaccggca aacccaaacc ctgccgatct
    21841 cgcccgaaca gcggcaactg ctcgccgaaa gcatgggttt cgacagttat gccgcttttt
    21901 cagacggcct caatgttcat cggaacaaag tcaatcagtt gttcaacgaa attttgagcg
    21961 aacctgaaga acaagcgcaa agcaatagcg aatggcaatg ggcatggcag gaaaaacccg
    22021 acgaagaaga acggctaggc cgtctgaaag aacacgggtt cgatgccgaa gccgtcaccg
    22081 caaggctcga acaaatctgc cacggacata aataccgccg cctttccgca cacgcccagc
    22141 cgcgctttga caccattgtg ccgctgttcg tacaggcggc ggcagagcaa aacaacccga
    22201 cagatacgct gatgcggctg ttggattttc tcgaaaacat cagccgccgc tccgcctatc
    22261 tcgccttcct caacgaacat ccgcaaacct tggcacaact ggcgcagatt atgagccaaa
    22321 gctcgtgggt ggcggcgtat ctgaacaaat atccgatttt gttggacgaa ctcatcagcg
    22381 cgcagctttt ggataccgcg tttgattggc aggcactcgc cgccgccctt tcagacggca
    22441 tcgaagcctg cggcggcgat accgaagcgc aaatggacac gctgcgccat ttccaacacg
    22501 cccaagtctt ccgcctcgcc gtccaagacc tcgccggatt gtggacggta gaatccctct
    22561 ctgaccaact ctccgccctc gccgacaccg tcattgccgc cgccctttcg tgcgcgtggg
    22621 cggatatgcc caaaaaacac cgcgacaccc cgcaattcgc cgtcatcggc tacggcaagc
    22681 tcggcggcaa agaactcggc tacgcctccg acctcgactt agtctatctt tacgacgatc
    22741 cccatcccga agcaggcgac gtgtacagcc gcctcgcccg ccgcctgacc aactggcttt
    22801 ccgccgccac cggcgcgggc agcctctacg aaaccgacct gcgcctgcgc cctaatggcg
    22861 acgccggttt cctcgcgcac agcatcgccg cctttggaaa ataccagcgt gaaaacgcat
    22921 ggacatggga acaccagtcc ctcacccgcg cccgcttcat ctgcggcaca cccgaaattc
    22981 agacggcatt cgaccgcatc cgcaccgaaa tgctgactgc cgaacgcgac caaaccgcct
    23041 tggcaggcga gatcatcgaa atgcgcgaaa aaatgttccc cacccatccg cctgccgaca
    23101 gcaacgtcaa atacgcgcgc ggcggcgtgg tcgacgtcga atttatcgtc caatatctga
    23161 tacttgccca tgcccgccag tatccgcaac tcttggacaa ctacggcaac atcgccctat
    23221 tgaacatcgc cgccgactgc ggtttgattg acaaaaccct cgccggacaa agccgcaccg
    23281 cctaccgcct ctaccgccgg cagcagcaca acaccaaact gcgcgacgcg gcaaaaaccg
    23341 aagtaagcga cgaattgctg tcccattacg gcaatgtcag gaaattgtgg cgggaagtgt
    23401 tcggggagga agcggcaacc gcttgaacaa aaaatgccgt ctgaagcctg acgattcggg
    23461 tttcagacgg catttgtttt tggctgtgtc tgaacaatcg gtacgatttc ggttgtttgc
    23521 aaattgaaag gcggttattc ctatgttcag gcggcaaacc gaaatatttt ccccaaccga
    23581 tagggcgata tcaaagccta ttcgaatacc gcctttacgg ggaaggggct ttccgagctt
    23641 gaccatggtc aggctcggca aatcagcccg catcctgtcg atgcgtccgt ccaaatcggt
    23701 ttggaacgcc gcaatgtgat atccgcctat caaggttttg cgtgcgtgtg tcggcgaggg
    23761 ctttgccaaa accgaaatca aaatcaagtt gtcaggcatc cttccgtttc tattaaaata
    23821 gcgcattcca tttttcagac ggcatcctta tgtttcccga ccaatccgcc cccaatctgc
    23881 tgcaaggctt gaaccccgaa caactctccg ccgtaacctg gccgccgcaa tccgccctcg
    23941 ttttggcagg cgcaggcagc ggcaaaacgc gcgtgctgac cacgcgtatc gcatggcttt
    24001 tgcaaagcgg acaagccagc gtacacagca ttatggcggt aacgtttacc aacaaggccg
    24061 ccaaagagat gcaaacccga ctcggcgcaa tgattcccat caacgtccgc gctatgtggc
    24121 tcggcacgtt ccacggtttg tgccaccgct ttttgcgcct gcaccaccgc gacgcaggcc
    24181 tgccgtcttc ctttcaaatc ctcgacagcg gcgaccagct ttccctcatc aaacgcctgc
    24241 tcaaaagcct caacatcgcc gaagaaatca tcgcgccgcg ttcgctgcaa ggctttatca
    24301 acgcgcaaaa agaatccggt ttgcgcgctt ccgtcttggg cgcgcccgat ccgcacacaa
    24361 gccgcatgat tgggtgctac gccgaatacg acaaaatctg ccaacgcgaa ggcgtggtcg
    24421 atttcgccga actcatgctc cgcagctacg aaatgctgca aagtaacgaa atcctgcgcc
    24481 ggcactacca aaaccgcttc aaccacattc tggtcgacga gtttcaagac accaacaaac
    24541 tgcaatacgc ctggctcaaa ctcatggcgg gcggtaacgc agcggtattt gccgtcggcg
    24601 acgacgacca aagcatttac cgcttccgcg gcgcaaacgt cggcaacatg accgcactga
    24661 tggaagaatt ccacatcgac gcgcccgtca aactcgaaca aaactaccgc tccgtcggca
    24721 acatcctcgc cgccgccaac gccgttatcg aaaacaacga cgaacgtctc ggcaaaaacc
    24781 tgcgcaccga cgccgaagca ggcgacaaaa tccgctatta ctccgccttt accgacctcg
    24841 aagaagcccg gttcatcgtg gacgaaacca aagccctcga acgcgaaggc tgggatttgg
    24901 acgaaatcgc cgtcctctac cgcagcaacg cccaatcgcg cgtcatcgaa caaagcctgt
    24961 tccgcagcgg catcccctac aaaatctacg gcggcctgcg cttttacgaa cgccaagaaa
    25021 tcaaacacgc cctcgcctac ctgcgcctcg ccgtcaatcc cgacgacgac aacgccctct
    25081 tgcgtgtcat caacttccca ccgcgcggca tcggcgcgcg caccgtcgaa aaccttcaga
    25141 cggcctcaaa cgaacaaggc atcaccctct ggcaggcagc ctgcaacgcc ggcgcgaaag
    25201 ccgccaaagt cgtcgccttc gtccgcctga ttgaagccct gcgcaaccaa gtcggacaaa
    25261 tgcacctgtc cgaaatcatc gtcggcatcc tcaaagacag cggtctgacc gagcactacc
    25321 gaacccaaaa aggcgacaac caagaccgcc tcgacaacct tgacgaactc gtcaacgccg
    25381 ccatcgagtt caaacccgaa gacagcaact ttgaaaccct gcccgaaaac atttcagacg
    25441 atcccgcctt ccccatcctc gccttcttga gcaacgccgc cctcgaatcc ggcgaaaacc
    25501 aagcaggggc aggcgaaaaa gccgtccaac tgatgaccgt ccacgccgcc aaaggcttgg
    25561 aattcaacgc cgtcttcctc accggtatgg aagaggggcg tttccccagc gaaatgagcc
    25621 ttgccgagcg cggcggcctc gaagaagaac gccgcctcat gtacgtcgcc atcacccgcg
    25681 cccgcaaacg cctctacatc accatggcac agcaacgcat gctgcacgga caaacccaat
    25741 tcggcatcgc atcccgcttc gtcgaagaaa tcccgccaga agtattgcac tacctgtccg
    25801 tcaaaaaacc cgccttcgac agctacggca acacgcgcca aaccaccgta caacgggaca
    25861 aaatcatcga tgacttcaaa cagccgcaaa cctacgcagg cttccgcatc ggacaaaacg
    25921 tccgtcacgc caaattcggc accggcgtga ttatcgatgc cgcagacaaa ggcgaatccg
    25981 cccgactgac catcaacttc ggcaaacagg gcgtgaaaga gctggatacc aagtttgcga
    26041 aattggaagc gatgtaaatt tgaaatgcag gccggatatt cgtatccgac aaaaaaacat
    26101 ttgacgcgtt tatcgtttcc gaaaaaccgc tgttggaaat gtcggattcg agaatccgac
    26161 ctacggcaaa aaaccgtagc aaggacaagg caaatagtcg tctgaaaacg ggaagggcaa
    26221 ttttgccgca gccgccgccg tcattcccgc gaacctacat tccgtcattc ccacgaaagt
    26281 gggaatccgg aacgcaaaat ctaaagaaac cgttttatcc gataagtttc cgcaccgaca
    26341 aaaccagatt cccgcctgcg cgggaatgac ggcggcggga atcggcggat tcggcggagt
    26401 tggtggattt ggcgggctga agcccgccct tgtatatcgg aacttccgta tcatagcgac
    26461 aaaccgcccg gccgccaccc gcgcccgccc aaggcagaca accgttgcgc ggctcaggga
    26521 gcggcagggc aacccat
//
LOCUS       DAXDZG010000023        26098 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXDZG010000023 DAXDZG010000000
VERSION     DAXDZG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26098
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.023"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(202..849)
                     /locus_tag="ACFB78_000844"
     CDS             complement(202..849)
                     /locus_tag="ACFB78_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC1268876.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            complement(846..1010)
                     /locus_tag="ACFB78_000845"
     CDS             complement(846..1010)
                     /locus_tag="ACFB78_000845"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC1268877.1"
                     /translation="MNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDF
                     LMKTGKGGKR"
     gene            complement(1012..1089)
                     /locus_tag="ACFB78_000846"
                     /pseudo
     CDS             complement(1012..1089)
                     /locus_tag="ACFB78_000846"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            complement(1223..1894)
                     /locus_tag="ACFB78_000847"
     CDS             complement(1223..1894)
                     /locus_tag="ACFB78_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC1268878.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQTAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            complement(2060..2779)
                     /locus_tag="ACFB78_000848"
     CDS             complement(2060..2779)
                     /locus_tag="ACFB78_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1268879.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2861..4174)
                     /gene="hpnE"
                     /locus_tag="ACFB78_000849"
     CDS             complement(2861..4174)
                     /gene="hpnE"
                     /locus_tag="ACFB78_000849"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC1268880.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(4249..5121)
                     /gene="hpnD"
                     /locus_tag="ACFB78_000850"
     CDS             complement(4249..5121)
                     /gene="hpnD"
                     /locus_tag="ACFB78_000850"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC1268881.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5292..7154
                     /gene="hscA"
                     /locus_tag="ACFB78_000851"
     CDS             5292..7154
                     /gene="hscA"
                     /locus_tag="ACFB78_000851"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC1268882.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7340..7988
                     /locus_tag="ACFB78_000852"
                     /pseudo
     CDS             7340..7988
                     /locus_tag="ACFB78_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            8024..8776
                     /locus_tag="ACFB78_000853"
     CDS             8024..8776
                     /locus_tag="ACFB78_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC1268883.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCVPVG
                     DISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEKEHP
                     ENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLEYGA
                     NPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            8832..9173
                     /gene="fdx"
                     /locus_tag="ACFB78_000854"
     CDS             8832..9173
                     /gene="fdx"
                     /locus_tag="ACFB78_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC1268884.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9233..9773)
                     /locus_tag="ACFB78_000855"
                     /pseudo
     CDS             complement(9233..9773)
                     /locus_tag="ACFB78_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            9986..10183
                     /gene="iscX"
                     /locus_tag="ACFB78_000856"
     CDS             9986..10183
                     /gene="iscX"
                     /locus_tag="ACFB78_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC1268885.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10251..10652)
                     /locus_tag="ACFB78_000857"
     CDS             complement(10251..10652)
                     /locus_tag="ACFB78_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC1268886.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            10864..11817
                     /locus_tag="ACFB78_000858"
     CDS             10864..11817
                     /locus_tag="ACFB78_000858"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC1268887.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFS"
     gene            11891..13201
                     /gene="tilS"
                     /locus_tag="ACFB78_000859"
     CDS             11891..13201
                     /gene="tilS"
                     /locus_tag="ACFB78_000859"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC1268888.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            13275..14060
                     /locus_tag="ACFB78_000860"
     CDS             13275..14060
                     /locus_tag="ACFB78_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC1268889.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADEILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14070..14267
                     /locus_tag="ACFB78_000861"
     CDS             14070..14267
                     /locus_tag="ACFB78_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268890.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            14529..14882
                     /locus_tag="ACFB78_000862"
     CDS             14529..14882
                     /locus_tag="ACFB78_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268891.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEEQ"
     gene            14894..15199
                     /locus_tag="ACFB78_000863"
     CDS             14894..15199
                     /locus_tag="ACFB78_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1268892.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKDCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            15261..16637
                     /gene="mpl"
                     /locus_tag="ACFB78_000864"
     CDS             15261..16637
                     /gene="mpl"
                     /locus_tag="ACFB78_000864"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC1268893.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            16844..17896
                     /gene="bioB"
                     /locus_tag="ACFB78_000865"
     CDS             16844..17896
                     /gene="bioB"
                     /locus_tag="ACFB78_000865"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC1268894.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(17992..18765)
                     /locus_tag="ACFB78_000866"
     CDS             complement(17992..18765)
                     /locus_tag="ACFB78_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC1268895.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLDAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            18755..18991
                     /locus_tag="ACFB78_000867"
     CDS             18755..18991
                     /locus_tag="ACFB78_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268896.1"
                     /translation="MVCTFFRRTAFVKQRTFKVADGNVRIFQDGFDVFQDIGIGRVID
                     RIIAAEHNAADPDDGNGICCLSVRRILSVRIAGV"
     gene            complement(19251..21110)
                     /gene="ilvD"
                     /locus_tag="ACFB78_000868"
     CDS             complement(19251..21110)
                     /gene="ilvD"
                     /locus_tag="ACFB78_000868"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC1268897.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(21283..23048)
                     /gene="cysI"
                     /locus_tag="ACFB78_000869"
                     /pseudo
     CDS             complement(21283..23048)
                     /gene="cysI"
                     /locus_tag="ACFB78_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(23075..24868)
                     /locus_tag="ACFB78_000870"
                     /pseudo
     CDS             complement(23075..24868)
                     /locus_tag="ACFB78_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            complement(24865..25947)
                     /locus_tag="ACFB78_000871"
     CDS             complement(24865..25947)
                     /locus_tag="ACFB78_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC1268898.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
BASE COUNT         5676 a         6844 c         7523 g         6055 t
ORIGIN      
        1 aatttaatcc actataattt accatacaac cacgccggaa ttaagtttaa atttgaataa
       61 aaggttcggg ttctgcaaaa tacagaaccc gaaccttgtt cggatattga aaccggtcgc
      121 ctgattttgg gcggtgcggc ttgcaagtat caagattcgc atatgccgtc tgaagctcgg
      181 agaggttcag acggcatatg cttatttggg ctgctcttcg acgaatctcg gacctttcaa
      241 gataccgttg cgggaatagg gcgacagtag gttgtatgcg gcggttttgg aaacctgata
      301 accgcggtcg gtcaggctgt tggcaatctg attgaccact gcgccgacca aagcccccaa
      361 caggccgctg ttgctgttgt tgctgccttc gcggatgctg gccgaacccg accacaactc
      421 tttcccgttg cgggaatcga ccagccgtgc tttggcggat acggtcgtca cgctgtctaa
      481 aatttgatat gaagtgccgt attcggtaac cgtaatgtac aaaaccgcat cattgccgaa
      541 aatttgatgc agtttttccg gccggacggc gtgaatatcg gcggcattgg tcaagccgtt
      601 ttctttgaag gtttcctcca cgactgcggc gggaaagacg taatagccgg cttcggaaat
      661 cggcgcggcg gtcgaagcca gcatccccca agtgccgttg acatcaggcg actcgttcag
      721 cggcggaacc accaaaattg aagccggttt gctttctttg aatgacgtgt agtcgaggtc
      781 gggagctttt cgaacttggc aggcagacag agccaacacg gcggcaagcc ctaaaatcaa
      841 aggtttcatc gcttgcctcc tttaccggtt ttcatcagga agtccataaa tacgcccgat
      901 tcgggaaaca gccttttctc ttcttcaaat tggcggaacg cgccctcttt gtctcccgaa
      961 cgggaaagca gcagtcccaa atgggcgtgc gcacccgggg cggcattcat tttttttgtt
     1021 ggcggcttcc gcaaagtatt tttccatctt ttcggtctgc ttgcccaacg aagtgtcgtc
     1081 gtttttcaaa ccttcataga cggtatcggg ataaccgccg taataataca ggtttttatg
     1141 cccgccgccg gcaggcggtc agagccaaga ccgccgcaca cagcgacaaa cggcccaagg
     1201 ttttcggatt catcatttct ccttaacggt tggattgcca tgcgccgttg tcgacagcct
     1261 gaaccaagtt gtcgacggct tcgcggattg ccaagtctaa aactttgccg ttcaaagtcg
     1321 catcgtagcc ggaagtgccg ccgaaaccga tgatttcgcg gttggaaagt gcgtattcgc
     1381 ccgcgccctg tgtggaatag acgatttcgg aagtattgac gttgacgata ttcagagcca
     1441 cttttgcata ggcggtttgc gatttgccgc gacccaaaat gccgaagagc tgatgatcgc
     1501 cgacatctct gcgtccgaat tcggttacat cgccggtaac gacataatct gcgcctttca
     1561 ggttctgcgc tttgccggaa atgccggatt cctgtttcaa tgcgctaagg ttggtgcggt
     1621 tcagtacgtt gaagcggttg gtttgttgca ggtgtgttac caggatggtt tttgcctggc
     1681 tgcccagacg gtcttcactg tcggagaaaa tgcctttttg gaagctggag cggttgtcga
     1741 atgttccgac ggaaatcggg gtgcgaacac cgtgatattg cgtattgcag gaggcgactt
     1801 ttgcaacctc gaggctgcgt gaggactcgg tcgcacagcc ggtcagtgaa acggcagcgg
     1861 cggcaaaagc gatggcggcg gaaacggttt tcataaaatt taccctaagg tccagttagg
     1921 gaaataacgg gttatcatta ttgtccttat gtaaatttaa gtcaaggtgt ttgtctgtgc
     1981 gggacggatg cgcgcggaag gtacggatat ttttcaaaac ggaagcgcgg cgcgggcggg
     2041 atgcctgcct tttccggatt tagaggtaaa cgatttcgcc gctccgccct ttgctttcgg
     2101 cacttgccca ccagacaaat gcgggcagta cgtccccgta gcttttgcgt tcgcttccgg
     2161 cttcgcccgg atgggatttg atgcgttgcg gggaattgat ggggccgggg acgaggacgt
     2221 tggcgcgcag gttgccgaag cgttcccatt cgtcggcggc gactttgcac aggtagttca
     2281 acgcggcttt ggacgcgccg aagccgcccc agtaggcttt gggtgtttcg ccgtggcttt
     2341 cgccgacgaa gatgacggac gcgtcgggcg actgcttcag cagcgggaac agggcgcggg
     2401 tcagccccat cggggcgacg gtgttgatgc ggtattggtt gacccattcg gcgacggttt
     2461 ggaaatccag cggcgagagg gcgtaaaaat agccggcgca gtggacgatg ccgtccagtt
     2521 tgccttgcgt ggcttcggca atggtggcgg caaaccgttc gaattctttt tcttccgcac
     2581 tcatcaggtc gaaacggatg gcgaagggtt cggggtgtcc ggcttcgaca atcgcgtcat
     2641 aggctttttc cagttttttc tgatggcgtg ccaccagaat tacggtcgcc ccttcggcgg
     2701 cgtaggcttt ggcgacctgt tcgcccaagc cttgcgatgc gccggtaact aagatggttt
     2761 tgtcggtcag tgtcggcatg gttgtccttt gggtttgacg gttaatgtat tttagcgttt
     2821 tgccgcacct tgtaaagcgt cccgatgccg gccggcgttt tcagacggca tcgttcaggc
     2881 tttgcaggca ggcttccgcc gacgcgaaac ctgattgtac ggcggcttcg agcgtggcgg
     2941 ggtagtgcgg gtggaggtag tcgccggcgg ggaagatacg gtgccggtgc agccacgaca
     3001 aatccggcgg cggggcatcg gttgcggttg tggcgcgttt ttcggtgatg acgcgcacgg
     3061 cttcgggttc gcccaaatgc ggaaggatgc gtctgaggtc ggcgtgggct ttgtccgccc
     3121 acgcccggtt tgcaaacgcg ccgacgcggt cggaaacgct aatgacggcg gagacttcgt
     3181 tttcgggcag tccgagcctg ccccggcaaa gcagccattg tgccgtgccg tcggcaatgc
     3241 cggtcagcgg ggcgggcagg cggacgggtt cggcgtagcg cagatagacg gtggtaatgg
     3301 cgtggtagcg aaggttttga tatgccgtct gaacgtgttc gggcgtgcct tcgggcagga
     3361 gcgcggcggc gtggtagggc gcggtggcaa gtatggcggc atcgaaggct tcgccgttga
     3421 cgaggacttt tccgtccggg agggtgttca gacggcatac gcgcgtttcg aggcggatgt
     3481 ccgcgccgag ccgttgaagc tccgccaagg cgggttcggc gacgattgcg cccaaatcct
     3541 gtttgggtag gagatagtcg ctgccggatt ttttcgtcag cacgccgtcg gacaaaacgt
     3601 tgcacaacac gcgcaggctt gcggtttcca aaggcgtgtt gagcgcgccc cagaccaagg
     3661 gctgccaaaa ctgcattacg gcggcacgcg gcacgttccg ctgtttcagc cattgtgcaa
     3721 ctgtcgtgtc gggctgtccg aggcgtgcgg acttctgcaa atcggacata tcggcaagca
     3781 gtttggcttt gaatgcggac ggtacgcgcc gggcaagcag cacgccgccc aaaatatgca
     3841 gcggcgcggg cagggggagg gcgcggaact gcaaaccgcc gtgcatatgc cagtgcagcg
     3901 gtacgcgcaa aaaggcggca cgggggtctg aaccgatggt tttcatcagg cgcaacacgc
     3961 cccggtatgc gccgagcaaa atgtgctgcc cgttgtccaa aaaaccgaaa ccgtcggtat
     4021 ttccggccag tgtgcgcgcc cttccgcccg cctgccggcc ggcttcaaac agggtaacgt
     4081 cggcgtgccg cgccaaggtg acggcggcgg acaagccggc ccagcctgcg ccgatgacgg
     4141 cgattttcgg gcgcggatgc ggcgtgttca tcatttattc ctccaatggt tgaaaccccg
     4201 tttttcgggg cggcgggggt tgggttttcc tgaagggcag tatgcgcctt atgcccgttc
     4261 cggcgtgccg gggcgcggtt tgaatccgaa taaccaggtt ttcagggcaa tgcgtttttt
     4321 gcgcggcgaa gggagggcga ttttgtattt gaggacgttt tgcgcgccgt ctcggtcgat
     4381 ttcgttcaac agcgcgtaat aaaccgccgc cataaccagt ccgacttttt gggctttttt
     4441 atcggcatca ggcagcagcg atacggcttc gcggtaggtt tcacgggcgc gtttgatttg
     4501 gaacgccatc aattcggcaa aattgcccgt cgggctgcat tgcaaaatca cgcttgcggg
     4561 tacgtcaaac cgctgcattt cctccatcgg caggtaaatc cgtcccctgc gcgcatcttc
     4621 gccgacatcg cggatgatgt tggtcagttg cagcgcaaga cccattttat ccgcgtattc
     4681 cagcgttttg ccgtctgaaa accccaaaat ccgcgcaatc aggcagccga ccacgcccgc
     4741 gacgcggcgg caatacagtt tcaattcctc aaaactgccg taacgcgcct gaaccaaatc
     4801 catctgcatc ccgtcgatta aggcttccag ttcatatttc ggcagcttga aggtttcctt
     4861 aacctgccgc aaggcctgat tgacggggtg ttccggcatc gcgccgccga acgcattgtc
     4921 caaatcgccg cgccaccagt tcaatgttgc ctgtgcaaca tcggggttgg aacattcgtc
     4981 aaccacatcg tccaattcgc ggcaaaagac atacagaacc gttaccgcat cctgtttttc
     5041 ctgagtcagg aaacggaagc ccgacaaaaa actggagcgg ctttcctctg ctttttggcg
     5101 gcaatagtcg agtcctttca cgatttatat tcctaatgat gggcgggaaa ggcggatttt
     5161 atcggtattt ggcggtagag ggcaatttcg gcggcacggc ccaatcctta gcggtttgct
     5221 caactatcgg cgcaaattct gttaaaatgc cgccttcctt cctttacaca ccgcaccgac
     5281 aggcaaaatt tatggctctt ttgcagattt cagaaccggg tatgtccgcc gccccgcacc
     5341 ggcaccgttt ggcggcaggc atcgatttgg gtacgaccaa cagcttggtc gccaccgtcc
     5401 gcagcggcag tgccgcctgc ctgcccgatg ccgacgggcg cgttaccctg ccttccgtcg
     5461 tccgctatct ggaaaacggc ggcattgaag tcggcaaaac cgccctgtcc gcccaaaaaa
     5521 ccgacccgct gaacaccgtc agctccgcca aacgccttat cgggcggact cttgccgatc
     5581 tgcatcaaaa tacgcattac ctgccttacc gtttcggcga caatcaacgc tttatcgaac
     5641 tgcatacgcg gcagggggtg aaaacgcctg tcgaagtgtc ggcggaaatc ctcaaaaccc
     5701 tcaaattgcg tgccgaagaa accttgggcg gcgatttggt cggcgtggtg attaccgtcc
     5761 ccgcctattt cgacgacgcg caacgccagg ccaccaaaga tgccgcgcgt ctggccggtt
     5821 tgaacgtatt gcgcctgctc aacgaaccta ccgccgccgc aatcgcctac gggctggaca
     5881 acgcctcgga aggcacgttt gtcgtgtacg atttgggcgg cggcacattc gacgtatccg
     5941 tgttgcaact gaccaaagga ctgtttgaag tcaaagccac cggcggcaac agcgcgttgg
     6001 gcggcgatga cttcgaccat cgtttgttct gctacctgct cgaacaaaac agactctccc
     6061 aactcaacga acaagacagc caactcctgc tctcgctcgt ccgcgccgcc aaagaacaat
     6121 tgaccacgca aaccgaagcg cgcattcagg cgacgttttc agacggtatg gcaatcgaca
     6181 caagcatcag tcgcgccgag ttccacaacc tgacgcagca tttggtgatg aaaacgttcg
     6241 agccggtcaa acaggcgttg aaagatgccg gtgtcggtaa aaacgaagtc aaaggcgtgg
     6301 ttatggtcgg cggttcgacc cgtatgccgc acgtccaaca ggcagtcgcc acctttttcg
     6361 gacaaacccc gctgaacaac ctcaatcccg acgaagtcgt cgcgctcggc gcagccatac
     6421 aggcaaacgt cctcgcaggc aacaaagccg acggcgaatg gctgctgttg gacgttaccc
     6481 ccttgtcgct cggtttggaa acctacggcg gcttggcgga aaaaatcatc ccgcgcaatt
     6541 ccaccatccc caccgcgcgc gcgcaggact ttaccacctt caaagacggt cagaccgcga
     6601 tgacgataca cgtcgtgcaa ggcgaacgcg agctcgtttc cgactgccgc agccttgcca
     6661 aatttaccct gcgcggcatt ccgcctatgg cggcgggtgc ggcgcgtatc cgcgttacct
     6721 tccaaatcga tgccgacggt ttgctgtccg tttccgccca agaacaaagc accggcgtac
     6781 aggcgcaaat cgaagtcaaa ccctcctacg gtttggacga tgacaccatc acccaaatgc
     6841 tcaaagacag catgggcaac gccgccgaag atatggcggc acgcgcccgc gccgaagccg
     6901 tggtcgaagc cgaaagcctg accgatgccg tcaacgccgc cctcgagttg gacagcgatt
     6961 tgctggatgc cgaagagttt gcacaaatcc agcgagacat cgccgatttg caaggccgtc
     7021 tgaaagacgg taaagctgaa gacatccgtg cagctgtcgc caaactcagc cgcagcaccg
     7081 acaatttcgc cgccaagcgc atgaaccgca acatccaacg tgcgctgaca ggtcagagtg
     7141 tcgataatat ttgatactta aacggtttca gacggcatag atataacctg atgccgtctg
     7201 aaggatcgaa aacacttgaa aaacatcgat atggaaaagt caggcattgt ctattcgatg
     7261 aaaaccgtca tcaagggcgt gtatagtgaa ttaaatttaa accggtacgg cgttggctcg
     7321 ccttgccgta ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt ttgttaattc
     7381 actatataaa atcggcattt cggacgtaag caattttgaa ggcagaatgc gccatttgga
     7441 aaacaacggc tatgcgaacg ttgccgggtt ggaacgcctc ctcgccgtca aaaccgacaa
     7501 ttacaaagaa aaaaaacctg ctccatgaaa ttttcagcaa aagcaggata ggcgataccg
     7561 aattgttcgc cgtggacgaa aaccttgtga aacgtttgtt tttatcgctt cgcggcgaaa
     7621 tcgtgttccc gaaaaacgaa acggcggaat cggaatttga aaaaagcgtc cacgaacgcc
     7681 ggcaggaagg gaatgccggg tccggccgca aacaactgct tgatttggta cggcgcggac
     7741 accgggaata cccttacgcg ctgccccggc ttttggcgga cgcggcatcc tacaagccga
     7801 aaaaatcgaa aatccgcctt tttaaagaag catattcccg caaaagcggc aggaggctga
     7861 ccgacgaaat tgcagacggc atccatattt acacctggtt ttcgcgggta aacttagaaa
     7921 aagcctattc cgaatatttg gaacttttca aatccgaatc ggatgccgga ggcagaaaac
     7981 cgcggtaagg tgcaaacaga taccgtacac gttgaggagc agtatgatgg gcgattccgt
     8041 catttattat gtagaacagg cagacgaacc ggtaaaccgt gccggcgaac gcgcccgtaa
     8101 aacattcaaa tacttttggc gcgagctttt ttgggaacgc cgccgcatta tttccgcctt
     8161 ggattttgcc atggtcaaag tccctttttt ccaagacggc gaaatttgcg aacatatgtg
     8221 gatcgacgat atttattttg acggtcttta catttacggt gtgctgaaca atgaacccgg
     8281 cggactgacc aatgtcgaac aaggcgaaag cgtttgcgtt ccggttggcg acatcagcga
     8341 ctggatgttc gtgtgcaacg gcatccccta cggcggcttt accgtacagg caatgcgcgg
     8401 acagatgacg gaagaggagc gtaccgaaca cgataccgcc tggggaatcg atttcggcga
     8461 tcccgggcag gtattgccgg tgtatgaaga aaaagaacat cccgaaaatt tggaagagca
     8521 tccgatgtgc cggaactgta ttgacgattt tcggcaacag ttgtcccaaa atccggattt
     8581 tctgcatgag caggacgaag acggctatac gccgcttcat catgaagcca tggcaggaaa
     8641 tgcacttatg gttcaagcca tgcttgaata cggcgcaaat cctgcctcaa agacatcgga
     8701 aggctatacc gccctcgatt ttgcccgcct gacgggctgg caaaatgttg ccgacctgct
     8761 cgaaccgcga cattaggcag acagttttcc taaaaacgga cacaaatact ttttacagaa
     8821 agacaataaa aatgcccaaa atcaccgtac ttccacacgc aacattatgc cccgagggtg
     8881 cagtcatcga taacgcgccc gaaggcaaaa ccgtcctcga cgtgctgctc gaccacgaca
     8941 tcgaagtcga tcacgcctgt gaaaaatcct gcgcctgcac cacctgccac gtgattatcc
     9001 gcaaaggttt cgacagtttg gaagagccgg ccgaattgga agaagacctg ctcgatcagg
     9061 cttggggttt ggaagccgat tcgcgcctga gttgtcaggc ggttgttgcc ggcgaggatt
     9121 tgattgtgga aatccccaaa tacaccatca accacgcgcg cgaagaacac tgaaaacagg
     9181 tcgtctgaag ccgacacgct tcagacggca ttgttgcgcg gataaggcgc aattaccgaa
     9241 aaacagacgt ttgtgaagac ggaactttcg attctatatt gatttttatc ggttttttga
     9301 cggaacgacc cgatacggca tctgaggcgg cttgcgtttc ttcctgcccg cctgccggat
     9361 ttgcctgtcc ttgcgcgaaa ccgaaagaga cggcggcgcg gcggacaagg tcgagatagc
     9421 gtccttcaag ctcggggcag gcggcggaca cgctttctgc cgcaaccgtg aagccgcccg
     9481 accgactcgg aaaggcgttc cgcaatctgc atgcagatgc gccaacgccg ggcgcgcaac
     9541 attgccgcat cgcaaccgac agccgcttta tccaaagcaa tcagaccgcg cctgctccgg
     9601 tagttaaggc aaaccgttac tctgtagagg gtatgttgcc cgtccaatat ttgatacagt
     9661 ttgcgtatca gcagaatcag gcggtcaaac tcctccatct ccgacaggga gtcgggatgg
     9721 tcggaagcgg tataaaccag ttcggacaat tttgcggcaa acatattgtt catcaatctt
     9781 ccttatcggt tgaaaccccg ctcttcaggg cggtagaatc agatttgttt gggaggggta
     9841 actcctcccg gatcaggacg acacataagg tggtgcttga tgtgccgtcc ggcgagttga
     9901 aacattcagc catcctcaag gggcggcagt tttgccgaaa catattctac acggcttcaa
     9961 tgccggacga taaaaggaaa tttatatgaa atggaccgat acccggcgca tcgccgaaga
    10021 actctacgac ctgcacggcg aagccatcga tcccaaaacc gtgcgcttta cccaactgcg
    10081 cgacctgatt atggcattgc ccgaatttga cgacgatccc gcccgttgcg gcgaacgcat
    10141 ccttgaagcc gtgcagcagg catggataga cgaggcggaa taagtttcag gaatgccgtc
    10201 tgaaatgcgg cggtacgcgg ttcgtgcttc tgtttgcagc gggaatggtt ttaccagtct
    10261 ccttttttca gcctgtccag ttggcggcgg tcgcgcttgg tcggtctgcc gtcgggatgg
    10321 gcggaagtga tgcggctgaa ttggtcgagc tgtttgcact cttccctcaa tgctgccgtt
    10381 ttcgcgtctt cttcatacag aagccgcgcc tcgggtgccg ggcggcgttg gtggttcaaa
    10441 cctttaacct tgattttatg gggaagggaa ttgagcgtca ggtcgataat atcgccgatg
    10501 tctatggttt tactgttttt gactttcgag ccgtttactt gaaccctacc cagttcgata
    10561 tgcttttgcg caagggaacg ggtcttgaaa aaacgtgccg cccaaagcca tttgtccagc
    10621 cgcatggcgg aagaatcgtg cttgtctttc atacgatttt gtttgaaata attgaatttg
    10681 tttcgagttt agcataagat acatcgcctt ataactagta tatatgcact aatccgctgt
    10741 tttccatgct gtccgaacac aaaaagaggg tgtggaaaag ccgttttgga caataaatta
    10801 actgcggaat atgcacaaat agcgtatgat agcggcagaa tctgttgatg agagcttcat
    10861 tttatgaaac ctgttttttt ggattttgaa caacccatag ccgaactgac caacaaaatc
    10921 gatgagctgc gtttcgtcca agacgagtct gccgtcgata tttcggacga aatacaccgt
    10981 ttgcagaaaa aaagcaacga tttgaccaaa tcgattttca gcaaactcac acccgctcaa
    11041 atttcacagg tttcccggca tccgcagcgt ccctatactt tggattacat tgacgcgctg
    11101 tttaccgatt ttgaagagct gcacggcgac cgccactttg ccgacgatca tgcgattgtc
    11161 ggcggattgg cgcgtttcaa cggacaaagc gttgtggtcg tcgggcatca gaaagggcgc
    11221 gacaccaaag aaaaaatccg ccgcaacttc ggtatgcccc gtcctgaagg ctaccgcaaa
    11281 gccctgcgcc tgatgaaaac ggcggaaaaa ttcggcttgc ccgtcatgac ctttatcgat
    11341 acgccgggcg cgtatcccgg catcggcgcg gaagaacgcg ggcagtcgga agccatcggc
    11401 aaaaacctgt acgaactgac ccgactgcgc gttcccgttt tgtgtaccgt catcggcgaa
    11461 ggcggttcgg gcggtgcgtt ggcggtcgcc gtaggcgatt acgtcaatat gctgcaatat
    11521 tcgacctatt ccgttatttc ccccgaaggc tgtgcttcga ttttgtggaa aaccgccgaa
    11581 aaggcggctg atgcggcaca ggctttgggt attactgccg accgcctgca aaagctggac
    11641 ttggtcgata ccgtcattaa agagcctttg ggtggtgcgc atagggattt cgggcaaacc
    11701 atgaaaaacg taaaagccgt tttggaaaaa caactgcacg aagcgcaaag catcccgctt
    11761 gccgatttgc tttcgcgccg tttcgaccgc attatggctt acggcaaatt ttcgtaacaa
    11821 taattcgggt agaacaagca gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg
    11881 ggatgaagcc gtgctgactt tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca
    11941 aggtctgaat ggaaaaaaaa cagcagtggc attaagcggc ggcttggatt ccgtcgtttt
    12001 gctgcatctg ctggtttgtg ccgggaagcg ggcaggattt gtgccggagg cattgcacat
    12061 ccatcacggc ttgagtcccc gagccgacga ttgggcggat ttctgccgga attattgcga
    12121 tatgctaggg gtggggctgg aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat
    12181 cgaggcggcg gcaaggcaaa agcgttatgc cgagtttgcc gaaaaaggct ttgacgtttt
    12241 ggcgttggcg caccacaggg acgatcaaat cgaaaccttt atgctggcag tcgcgcgcgg
    12301 cggaggtttg cgcgctttgg cggctatgcc cgccgtccgc cctttggggg aaaacggcat
    12361 tatctggcgg cctttgctgc ctttttcgcg ccaagacata tgggattatg cccgaaaaca
    12421 cggtttgccg aatatcgagg atgaaagcaa taccgatacg gcttatttgc gaaaccgctt
    12481 ccggcaccgt attttgcccg aactttcggc gcagattccc catttcgggc ggcatgtgct
    12541 gaacaatgtc cgcgctttgc aggaagattt ggctttgttg gaagaggtcg tcgttcaaga
    12601 ctgccgttgg gtttgcgggg ccggttattt cgatacggcg cggtggctga cgttttcccc
    12661 gcgccggaaa acccatattt tgcggaattt tctaaaggaa aacggcatcc ccgtgccgaa
    12721 tcagaacgca cttgccgaca ttgcccgcgt tttgacggag gcaaaaaccg gacgttggaa
    12781 cttgcaaggc tttgaattgc atcattatgc aggcaggctg tttgtgtttg cgtcggaaca
    12841 gttggcaaaa ccggcttttc tgaaagatgg gacgataagc ggaaatctca aaaaaatact
    12901 gacagaacat agatttgtgt tgaagcggca tccgttcgga cttcctgaag ccatgttgga
    12961 acaggacggg attttgagga cggtagcggc atcggatacg ttggctgtcg gcggcatcca
    13021 taaaaatgtg aaaaaaatcc ttcaggggaa acgggttctg cctttcctgc gcccaatttg
    13081 gccgctcgtt gccgacagcg gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt
    13141 tcaaatctcg gtttcagacg gcattttgcc cgtacatcct gactttccca ttttattttg
    13201 ataatatcgc aaacagattt cggcggcgtt cagtcgggta ttgtccggtt gcatatttct
    13261 aaaaggcttg tgaagtgaaa cacatcagtt cgaccaataa tgaacatatc agacacctgc
    13321 accgcctgtt gtcgcaagga aagttcagac ggcagtatgc ccaaaccgtt ttggagggcg
    13381 tgcacctgct tcaggttttc ctgcaatccg gcagaaagcc ggtcggggta tatattcccg
    13441 aagctaaaat gccgtctgaa gaagtcctta aattgacagc ggttttgccg gaagacggga
    13501 ttttttccgt ttcagacggc atattgaaaa aaatcagcag cttgagttgt gcagacgaga
    13561 tacttacact gattgatatc ccattgggcg gaaccttgcc ggacaaaggc gactgtgcgg
    13621 ttttggacgg cgtgcaagat ccgggcaatg tcggcaccgt cttacggagt gcggcggcgg
    13681 caggggtagg tacggtcgtt ttgggcaggg gttgtgcgga cgcgtggtcg cccaaggtat
    13741 tacgcgcggg tatgggcgcg catttcttgt tggacattta ttcgcaggcg gatttggaaa
    13801 tatggttggc gcactatgaa gaccgtgtgt ttgccaccgc gttgcgcgag gaaaagcagg
    13861 cggttttgta cggcgaagat ttgtgcgaac cgacagcttg ggtgttcggc aacgaaggtg
    13921 cgggtgtcgg taaagcagtt ttagacaggg cggacaagtg tgtcaggata ccgatgcacg
    13981 atgcaaccga gtctttaaat gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc
    14041 gccaacgggc ggcgtattga ggaagagaaa tgccgtctga aaaaatctat tacggcgtat
    14101 tgattttctt atgtatcgct tctatgctgc tgtcaccgtt tttttatgcg ggtgctttga
    14161 agcccaagaa ggcggcattg cggaaggacg ggcagtggaa actcatctta tcgtccaatg
    14221 ccgtggcggc ggcggttttg ttttggatat ggtggaaatg gttttgacag atattgctca
    14281 aaatcgtgct aatggaatcc gaacaaataa agaatttggt aaaaaatttg ttaaatcaac
    14341 ggattaaagt tttgtggaaa acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat
    14401 acggaagagt tgcggcatta tcgggcatct ttaacaagta gtgtcggctt ggcaggcaat
    14461 cggtttttat gggcagcttg caaaatcgcg gatataaaat tgcgaatcgg ttaaagtgtg
    14521 gggacgctat gaaaaattgc gaattttttt atgatccgac aagggcaatc tatgatagcg
    14581 gtgcagatta cttaactagg gaaaaacata gattagtcgt aatcgcaaat agcgcttggg
    14641 ggctattgct gaatttacct tgttattatg acgaggtttt ggaaaagcgg aaaataccgt
    14701 tcggcaaaca ggaaattgat gacgatatgg acaaagtgtc cgcccttaag cggaagttta
    14761 aagacatttc tgaaatcaaa gtaggggatg gttgggaata cccgttcaat tatgagcaag
    14821 gaatgaaaga attagatgaa gttctattga aatacattcc tttttttgaa gaggaacaat
    14881 aaaggagttt aatatgcgcg tatctaaaat gattggaagt atattgcttg ttgcagcggt
    14941 tcagaccgta ttttcggcaa atgtttacga gtgccgccat aatggtaaaa ccagttacag
    15001 ccaaactccg ggaaaagatt gtaccaacgc gggtttgggg cgggatcggg tgtacagttc
    15061 ggtcaggcct gccgtgaaag acagggcgga agacgcagga gttggcgatt attcggacac
    15121 ggtgagggac gaagccgtcc aaaatccgaa agggaatgca cagaaagacg gttcggatgc
    15181 cggcatcaga ccgcattgat tgaaaccgaa tcaaccatta cggtgttgaa caatagaatt
    15241 tgaacgatta gggaactctg atgaaacaca tccacattat cggtatcggc ggcacgttta
    15301 tgggcgggat tgccgccatt gccaaagaag ccgggttcaa agtcagcggt tgcgacgcga
    15361 agatgtatcc gccgatgagc acccagctcg aagccttggg cataggcgta cacgaaggct
    15421 tcgatgccgc gcagttggaa gaatttcaag ccgatattta cgtcatcggc aatgtcgcca
    15481 ggcgcgggat ggatgtggtc gaggcgattt tgaaccgtgg gctgccttat atttccggcc
    15541 cgcaatggct ggctgaaaac gtgctgcacc atcattgggt actcggcgtg gcagggacgc
    15601 acggcaaaac gaccaccgcg tccatgctcg cctgggtctt ggaatatgcc ggactcgcgc
    15661 cgggcttcct catcggcggt gtaccggaaa atttcggcgt ttccgcccgc ctaccgcaaa
    15721 cgccgcgtca agacccgaac agcaaatcgc cgtttttcgt catcgaagcc gacgaatacg
    15781 acaccgcctt tttcgacaaa cgctccaaat tcgtgcatta tcgcccgcgt accgccgtgt
    15841 tgaacaatct ggaattcgac cacgccgaca tcttcgccga cttgggcgcg atacagaccc
    15901 agttccacca cctcgtgcgc accgtaccat ccgaaggcct catcgtctgc aacggacagc
    15961 agcaaagcct gcaagatact ttggacaaag gctgctggac gccggtggaa aaattcggca
    16021 ccggacacgg ctggcagatt ggtgaagtca atgccgacgg ctcgttcgac gtattgcttg
    16081 acggcaaaaa agccggacac gtcgcatggg atttgatggg cggacacaac cgcatgaacg
    16141 cgctcgccgt catcgctgcc gcacgccatg ccggagtcga tgttcagacg gcctgcgaag
    16201 ccttgggtgc gtttaaaaac gtcaaacgcc gcatggaaat caaaggcacg gcaaacggca
    16261 tcaccgttta cgacgatttc gcccaccacc cgaccgccat cgaaaccacg attcaaggtt
    16321 tgcgccaacg tgtcggcggc gcgcgcatcc tcgccgtgct cgagccgcgt tccaacacca
    16381 tgaaactcgg cacgatgaag tccgccctgc ccgcaagcct caaagaagcc gaccaagtgt
    16441 tctgctacgc cggcggcgcg gactgggacg ttgccgaagc cctcgcgcct ttgggctgca
    16501 ggctgcgcgt cggtaaagat ttcgatacct tcgttgccga aattgtgaaa aacgcccgaa
    16561 ccggcgacca tattttggtg atgagcaacg gcggtttcgg cggaatacac accaaactgc
    16621 tggacgcttt gagatagccc gggcaatgcc gtctgaaagc cctttagacg gcattgcccg
    16681 gctgcgcggc acaaaggcgg aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg
    16741 ccgccgctgt tttcagccat ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc
    16801 ctgtcagcgt cattgcgccg ctgtaaatac gaaagaacac attatgaccg tatcccccgt
    16861 cgccttgcgc cgtaagaccg agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg
    16921 cgatgtcgag gcacttttcg gactgccgtt tttagaactt gtctatcagg cggcagaagt
    16981 ccaccgccaa aatttcaacc cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac
    17041 cggcggttgt cccgaagatt gcgcctactg tccgcaatca gcgcaccaca acaccaattt
    17101 gggcaaagag cagatgatgg atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc
    17161 gcgcggcgca agccggtttt gtatgggcgc ggcatggcgc ggccccaaac ccaaagacgt
    17221 ggagacggtt tccgcaatca tcaaagccgt caagggctta ggtatggaaa cctgcggcac
    17281 gttcggtatg ctcgaagaag gtatggcgga agacttgaaa gaggcgggct tggattatta
    17341 caaccacaac ctcgacaccg accccgaccg ctacaacgac atcatccaca cacgccggca
    17401 cgaagaccgt atggatacct tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg
    17461 cggcatcgtc gggatgaacg aaacccgcgc cgaacgcgcc gggctgattg ccagcctcgc
    17521 caacctcgac ccgcagcccg aaagcgtgcc gattaaccgg ttggtcaaag tggaaggcac
    17581 gccgcttgcc gatgccgaag atttggattg gaccgaattt gtccgtacgg tttccgtagc
    17641 gcggattacg atgccgcaaa gttatgtccg cctctcggca gggcgcagca atatgcctga
    17701 agcgatgcag gcgatgtgct tcatggcggg tgcgaactcg attttttacg gcgacaagct
    17761 gttgaccaca ggcaatcctg atgaggacgg cgatagaatc ctgatggaaa agcttaacct
    17821 gtatcccttg cagtttgagc cggaaggcga ggtcgccgaa gtggaaaaag cctccgggat
    17881 taaagcggat tattgacgat tgaaaaatgc cgtctgaaac ttgaatgccg tctgaaacct
    17941 gaaaaaaggc tttcagacgg catttgttcg gacggaattt ccaatatctt tttaccggcg
    18001 cgtgatgtcg ccgccgggcg agacatccag tccgttcccg ttggggaagt cgctatggtt
    18061 cagataaata atccgctcaa taacagtttt ttcagggtcg attttgggga ttttatcgcc
    18121 gacttgagtg atggggataa tgttgccgga aacgaaccgc ccctgtttgt cggtgataat
    18181 tttgaaaatc ggcgcaatgc cgctgatgcc gtaggtggca aagttgccgc cgctgtaaga
    18241 gatgaagcgg tcgtggtaaa gttcgacggc gcgagtaacg tgcgccccct gcccgaatac
    18301 gacatccgcg ccggaatcga cggcgagccg cgcaaactcg acgacgttgc ccctgttttc
    18361 cccatagaag atttcggtat cgaacggcag gtgttcagcc tgtttccctt ccgcgccgcc
    18421 gtggaacatc acaatgacga tgtcggtttt ttgtttggtt ttccgaatca gttttttgaa
    18481 tttggcgtaa tcgttcagtt tgacggcgtc aaggttgggg gcgaaggaga cgaagccgta
    18541 tctcacgccg tttttcttca ggatggcggt ttcaaacctg ttttcgatgc ccgaatattt
    18601 gatgttcaat tcgtcaaggt tgcctgccgt tgccgtgatg ccttgcgcgc cgaagccgtt
    18661 gctgtggttg tttgcgaggc tgaggtagtc gaatcccgcg tcggcaaggt attgcccgta
    18721 tgcggagggc gttcggaatg catagcatat tttggtggtt tgcacatttt ttcggcgtac
    18781 cgccttcgtc aaacagcgta ccttcaaggt tgccgacggt aatgtccgca tcttccaaga
    18841 cggattcgac gtgtttcagg atattggtat cgggcgggta atcgaccgga taattgctgc
    18901 cgagcataat gccgccgacc ccgatgatgg aaacggtatc tgctgccttt ccgtcaggcg
    18961 tatcctgtcc gtccgcatcg ccggtgtttg aaacgggcgc ggggtttgaa ccgtcaatcg
    19021 gcagcacggg ggattccgtt ccggcaggat gcgccgaggc ttgaccccgg cggttttcgg
    19081 cgcggaaaca gcataagggg cggcaggcgg ctcttttaca tactcgagcg cggtaaagcc
    19141 gctgccgata acgagggcca aaattgcgga aaataccgcc atcgcccaga taaatttgtt
    19201 aataatcatg cctttactgt tcagacggca tttgccgcac gttttggggc ttatctctcg
    19261 atttgcgata cgtcgcgcac cgcgcctttg tcggcggaag tcgccatcgc gccgtaagcc
    19321 cttaatgcgg cggagacgta gcggtcgcgg ttttccggtt tccacgcttt gctgccgcga
    19381 gcttccattt cggcacggcg tgcggcaagc tcttcatcgg aaatgacaag gcggatgctg
    19441 cgtttgggga tgtcgatttc aatcgtatcg ccttcgtgta ccaaaccgat tgcgccgcct
    19501 tccgccgctt cgggcgaggc gtgtccgatg gacaaacctg atgtgccgcc ggagaagcgt
    19561 ccgtcggtta agagggcgca ggctttgccc aagcctttgg atttcaggta ggaggtcggg
    19621 tatagcattt cctgcatacc cgggccgcct ttcgggcctt cgtagcggat gatgacgatg
    19681 ttgccggcga cgatttggtt gcccaaaata ccttcgacgg cggcttcctg gctttcaaac
    19741 acgcgcgcgc ggccggtgaa tttgaggatg ctctcgtcca cgcctgcggt ttttaccacg
    19801 cagccgcgct cggcgatgtt gccgaataag accgccaaac cgccgtcttg cgagtaggcg
    19861 tgtgccacgt cgcggataca gcctttttcg cggtcaaggt cgagggtttt ccaaatacgg
    19921 ttttgcgaga acgcctgagt ggtgcgcacg ccgccgggag ccgctttgaa acgttcgatg
    19981 gcgcgggtgt tttcgggatt ggtgacgtcc catttttcaa tcgcgtcttt cagtgtcggc
    20041 gcgtggatcg tgtacacgtc ggtgtgcagt ttgcccgctt tgtcaagttc tttcagaatg
    20101 gcgaagatgc cgccggcgcg gtgcacgtct tccatatagt agtcgtggtt gttgggcgcg
    20161 gttttgcaga tgcagggcac gacgcggctt aagcggtcga tgtctgccat tttgaaatcc
    20221 acgcccgctt cgttggcgac ggcgagcaag tgcaggatgg tattggtgct gcctcccatc
    20281 gcaatatcca tcgtcatggc gttttcaaac gcttttttgg tggcaatgct gcgcggcagc
    20341 acggtttcgt cgtcttgctc gtaatagcgt ttggtgattt cgacaatcat acgccctgct
    20401 tcgaggaaca attctttgcg gcctgcgtgg gtcgccaaat acgaaccgtt gccgggcagg
    20461 gacagaccga gcgcttcggt caggcagttc atcgagtttg ccgtaaacat acccgaacac
    20521 gagccgcagg tcgggcaggc gttttgttcg acttcttcga cttgccggtt gctgacattg
    20581 tcgtccgccg attcgatcat ggcgtcaatc aagtccaaac ggcgttcggg ttggatgttt
    20641 gccacgccga tgaccttgcc cgcttccatc gggccgccgg agacgaagat ggtggggatg
    20701 ttcaggcgca tggcggcaat cagcattccc ggggtgattt tgtcgcagtt ggaaatgcac
    20761 accagcgcgt cggcgcagtg ggcgttgacc atatattcga tggagtcggc aatcaaatcg
    20821 cggctgggca gggagtacaa cataccgctg tgccccatgg cgatgccgtc gtcgatggcg
    20881 atggtgttga attctttggc aattgcaccc gctttttcaa tctcgcgggc aaccagctgg
    20941 cccatattgt gcaggtggac atggccgggc acgaattggg tgaaggagtt ggcaacggcg
    21001 atgatgggct tgccgaagtc ggtttccatc acgccggtgg cgcgccacaa tgcgcgcgcg
    21061 cccgccatat tgcggccgtg ggtggaggtt ttggagcggt attctggcat agtgtgtttc
    21121 cttgtgccta taccgtctga aaggcagggc tgtttcagac ggcatcgggt acggtttttt
    21181 agagtgggaa aagggggtat tttataccaa gtatcggaat tttgcggaat ggaaacgtgt
    21241 gcggcaaaaa agaacatccc cgcaggaatg cgggggacgg gttcaggcgc gcgcaatcgg
    21301 gacggctttg gatgcgtccc aaaaatcgac gggcgcatcc aacacgggtt tgacgatgcc
    21361 cgtccgtatg gcgaaatcgc cgaaaccttc gtcggtatcg cgtcccgccg cccatttgcc
    21421 gatcaggtct tccaattcgg aaaggatttc cggcagggtg atgttttctt tgtagaggcg
    21481 ggggatgcgt acgccttcgc ggtcgccgcc gatgtggagg ttgtagcgtc ccacggcttt
    21541 gccgaccagt ccgatttccg ccaacatcgc ccgtccgcag ccgttcgggc agccggtaat
    21601 gcgggtaacg atgtagtcgt ccgacgcgcc gtgttccgcc ataatcttat ccagctcgcc
    21661 gatgaagtcc ggcaacacgc gttcggcttc cgccattgcc agcgggcagg tcgggaagga
    21721 agcgcacgac atcgcatttt cgcgcagctt gctgacaccg tcgcgtatca gcccgtatga
    21781 gcgggcgagc tgttcgatcc gggctttgcc tccttcgggc acatttgcca cgatgaggtt
    21841 ttggttggcg gtgatgcgga agccgccttt gtggattttg gcgatttcca acacgccggt
    21901 cagaagctgt ttcccgcctt cgtcaaccaa acgcccgctt tcgatgaaaa gggtcaaatg
    21961 ccagttgccg tctatgcctt tcacccggcc gatgcggtcg ccgcgcccgg taaacttgaa
    22021 cggccgtatg ggtttgaacg gcatacccat acggcgttcg acttccgtgc ggaagttgtc
    22081 caagcccata ttttgaatgg tgtagcgggt gcggtcgttt ttgcggtggc tgcggttgcc
    22141 gaagttgcgc tgcgtggtta ccaccgcttc ggcggccttc agtgcgtgtt ccggaggcac
    22201 gaaacccagt tccagtgaaa tgttcggata ggttttggtg ttgccgttgc cgtgttccgt
    22261 cgaaagcccg ccgcccgcca aaacattgaa gccggcaagc tgtccgttgc cgtctgaaac
    22321 ggcgacgaaa tccaaatcgt taccgtagca gtccacatcg ttcaagggcg ggatgacgac
    22381 tgcggttttg aattttcgcg gcagataggt tttgcccaaa atcggctcgc cttcttgaag
    22441 gaagtcgtcg gaactttgaa cttttttgcc gtccacccac atatccagat aaccgcgcgt
    22501 gcgcggcagc aggtgttcgg aaatcttttt cgcgtattcg taagcctgcc ggtgcagctc
    22561 ggactcgatc gggttggagg tgcaaagcac attgcggttc atatccgccg ccgtagcgat
    22621 ggaatccaaa cccaggctgt gcaagaggcg gggcatcgtc tgcaacttgg ttttcggcac
    22681 gccgtgaaat tggaaggttt gccggttggt tagccggatg gagcggtaat gactcttttc
    22741 ccgggcgaat ttgtccagtt ctatccactg ggacggtttg atgatcccgc cggcagccgg
    22801 cagcgcaaaa gcataaattt caagggctcg agttttgcct cggtgcggat gtcgcggcca
    22861 tcctgctcat acatactgtg gaagcggatg agttggaagt tgtcgccttt gaagccgccc
    22921 gtgagcgggt ctttcaaatc gtccaaaatc gtgccgcgta aaaaattgct ttcggttttc
    22981 aggcgttcgt tgtcggatag cggcttttct tgccacgcca aaccttttgt cttggcctgt
    23041 acggtcattt tgtgttcctc ccgattatat ttaatcaata aacatcacgc tgatagcgtt
    23101 tttcttcgcg cagcatatcc aaatatcctt ctgcgccgtc ttcgtccgaa tgccctgccc
    23161 cgataatcac atccagcaag gcggcttcca cttcttttgc catttttgcc gcatcgccgc
    23221 acacatagat atgcgcgcct tcctgcagcc attgccaaag tccttccgcc tgttcgcgga
    23281 ttttgtcctg cacatagatt ttttcttcct gatcgcgcga ccaggcgaag tcatatctgt
    23341 gcaggaagcc gtctttggca aactgctgcc attcggtctg atagaggaag tcggcggcaa
    23401 aatgcggatt gccgaaaatc agccagtttc tgccttccgc attttctgcg gcacgttgtt
    23461 ggacgaaggc gcggaacggt gcgacgccgg taccggagcc gatcatcaca atcggcttgc
    23521 ggctgtcttc gggcagcctg aagccgtcgt tgcgttccgc aaacacgcgc accgtgccgt
    23581 cctcttccag ccggtcggca aagaaacccg atgcgccgcc cgacctggcg cgcctttcgt
    23641 gttcgaaacg cactgcgccg acggtcaggt gcacttcgtc ccccgcttcc gcctgcgacg
    23701 aggaaatcga atacaggcgc ggcgcaagcg ggcgcagcag gccggcgaat tgttccgccg
    23761 tcagttttgc cgggaagcgg tgcagcacac cggcaatcgg cgtgctttgc acaaagcctt
    23821 gcaaaacggc gttgtcggca gcaatacggt cgagttcgtc atcatcggcg aacgtggcat
    23881 agcctttgac aaaggcgggg gtgttttgcg tgagttcgaa atgggataac agtgcggagg
    23941 caaccggcag ggtttttccg cccgcctgta tttccgttgc cggattgatg ccgagcaggt
    24001 ctaggatttc ccagaccagt gccggatcgt tgtcaaacca aacgccgagc gcgtcgcccg
    24061 ggaggtagtg caaatccgaa ccgctcaaat cgatttcgat gtggcgcacg tctttatcgg
    24121 attggcgggc ggtgattttc tgattggcca gcagggcggc gggaaagggg tctgccttgc
    24181 agtacctgcc ctccgatgcc gtctgaaggc cggcgggggg acgttgcctg cggcgcgggc
    24241 gttgcccggt tttttgcggc ttcttctttt aagcgcgcga caatgcggcc tgtccatccg
    24301 tctgcggcgg cggcaaagtc caaatccgca tcaacgcgtt cgagcaggcg ttttgcgccc
    24361 aattcttcaa aacgtttgtc gaaatctttg cccgcccggc agaaattcgg ataggagctg
    24421 tcgcccaaac ccagtacggc aaattggagt ttgtccaatt tcggggcttt tttgcagttc
    24481 agcagtttgt gcagcacgac ggcttcttcc ggcggttcgc cttcgccctg ggtggacgta
    24541 accagcagca ggcggcgttc gccggcgatg tttttcgcct tgtagccttt cagttcggcg
    24601 cgcctgactt ggatgccggc ggctttcagg ctgtccgccg ctttgtcggc aacggatttc
    24661 gcattgccgg tttgcgaggc ggaaaggacg gttacggaaa aaggttctgc cgccggcaat
    24721 gccgtctgaa gcgcgggcag ccctgcggat gccccgtttc ctgcttttgc ccaagcgtag
    24781 ccggacagcc ccgacacagg agctgcgtga tttcgggcgg cataggcggt aatggcggat
    24841 ttgtgttctg catatcgtgt tcactcataa aatcatacct gccgcaacag taccgtatgt
    24901 cgcttcgtct atcaggataa acgaaccggc ggcggtgttt tccgcataag gcgttgccgt
    24961 aacgggtttt tgaaggttga tgcggacttt ggcgatgtcg ttcatcttta aggattccgc
    25021 gccggcctct tgttccagcg tgcggacatc caaaacgctt tcaatttccc cgacttttgc
    25081 cggcacggtt tgcgtgccgt gcttgagcag gtatttgcgc gcggtgttga gcgggcgttc
    25141 gtcaaaccag caaagcgtgg cttccggatg tttttgcggg gcgagcggga aatttttatc
    25201 gacaaaaaga tcgccgcgcg aaacatcgat gtcgcggtcc agccggatgg ttgccgcctc
    25261 gccggcaaaa gcctgcgcca cttccccttt cggcgcgatg atttcggaca cttcggcggt
    25321 cagcccgttc ggttcgatgc ggacggtttg cccgacggtg accgaaccgc gttcgatgcg
    25381 cccctgatag cctcggaaac cgtcggcctt gtcggcatct tggcggacga ccagttggac
    25441 ggggaaataa aaaccggcat cgcgccacct tcctgaaatt tccgccatat cgcccaaaga
    25501 cggcggcagc aaggcttcca gcctttcacg cagcaggcgg gcgaggacgg gcgcgctgcc
    25561 ggagctggaa acggcaatct gaacagggtc gcggtcgata accgacggga agatgaagct
    25621 gcaatggtca cggtcgccca ccacgttgac cggcttttgg cagctttcgg caagatggaa
    25681 aacgcgccgg ttgagggctt ggtcgctgct tgccgcaatg atgaggaaaa ccgtgcggat
    25741 gtgttcggca cgaaattctt tggcaagcca caggattttg ttttccgccg ccaacgcgga
    25801 gagttcggca ttcaggtgtt ttgcggcaac cccgacctct gcgcccgcct tcggcagcag
    25861 gctgattttg cgtgcggcga ccgcgccgcc gcctacgacc aatacggggc ggccggagag
    25921 gttggcgaaa atgggaaaat aattcactgg ctgactcctt tgctgtttgc ccgcaccttg
    25981 tttccgatac ggtgcgtcgc ggcatttttg tcggaatgcg ggtcatttta aataataaaa
    26041 aggcatttgt tagaagctga aagctatatg gggggcggct gcggatgcgg cggttttc
//
LOCUS       DAXDZG010000024        25130 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXDZG010000024 DAXDZG010000000
VERSION     DAXDZG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.024"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..102
                     /locus_tag="ACFB78_000872"
                     /pseudo
     CDS             1..102
                     /locus_tag="ACFB78_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            280..465
                     /locus_tag="ACFB78_000873"
                     /pseudo
     CDS             280..465
                     /locus_tag="ACFB78_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(505..2433)
                     /gene="rpoD"
                     /locus_tag="ACFB78_000874"
     CDS             complement(505..2433)
                     /gene="rpoD"
                     /locus_tag="ACFB78_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC1268899.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(2620..4392)
                     /gene="dnaG"
                     /locus_tag="ACFB78_000875"
     CDS             complement(2620..4392)
                     /gene="dnaG"
                     /locus_tag="ACFB78_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC1268900.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(4537..7287)
                     /gene="secA"
                     /locus_tag="ACFB78_000876"
     CDS             complement(4537..7287)
                     /gene="secA"
                     /locus_tag="ACFB78_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC1268901.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7419..7841
                     /locus_tag="ACFB78_000877"
     CDS             7419..7841
                     /locus_tag="ACFB78_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC1268902.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            8124..8675
                     /gene="azu"
                     /locus_tag="ACFB78_000878"
     CDS             8124..8675
                     /gene="azu"
                     /locus_tag="ACFB78_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC1268903.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            8770..9279
                     /locus_tag="ACFB78_000879"
     CDS             8770..9279
                     /locus_tag="ACFB78_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC1268904.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            9313..9933
                     /locus_tag="ACFB78_000880"
     CDS             9313..9933
                     /locus_tag="ACFB78_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFC1268905.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            10046..11191
                     /gene="dapE"
                     /locus_tag="ACFB78_000881"
     CDS             10046..11191
                     /gene="dapE"
                     /locus_tag="ACFB78_000881"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC1268906.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGSLTVKGKQGHIAYPHLAVNPIHTFAPALLELTQ
                     EIWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            11611..12426
                     /locus_tag="ACFB78_000882"
     CDS             11611..12426
                     /locus_tag="ACFB78_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1268907.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTFDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(12404..12961)
                     /locus_tag="ACFB78_000883"
                     /pseudo
     CDS             complement(12404..12961)
                     /locus_tag="ACFB78_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            13311..14063
                     /locus_tag="ACFB78_000884"
     CDS             13311..14063
                     /locus_tag="ACFB78_000884"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC1268908.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            14254..14329
                     /locus_tag="ACFB78_000885"
     tRNA            14254..14329
                     /locus_tag="ACFB78_000885"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14287..14289,aa:Asn,seq:gtt)
     gene            14460..15470
                     /gene="waaF"
                     /locus_tag="ACFB78_000886"
     CDS             14460..15470
                     /gene="waaF"
                     /locus_tag="ACFB78_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC1268909.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            15525..15971
                     /gene="smpB"
                     /locus_tag="ACFB78_000887"
     CDS             15525..15971
                     /gene="smpB"
                     /locus_tag="ACFB78_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC1268910.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            17029..18372
                     /locus_tag="ACFB78_000888"
     CDS             17029..18372
                     /locus_tag="ACFB78_000888"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1268911.1"
                     /translation="MGALELTNSLWEPRWNSNIDYLITKNAEIRFNTKNESLLVKEDY
                     AGGARFRFAYDLKDKVPEIPVLTFEKNITGTSDIIFEGKALDNLKHLDGHQIVKVNDT
                     ADKDAFRLSSKYRKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLND
                     KNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYVDSWMQYQ
                     RFRHRINTEYATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGV
                     NGKFSDSENAQVNLLGSRQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVE
                     IDGDRRVINNKTVIETQLGVAAKIKSHLTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(18355..18660)
                     /locus_tag="ACFB78_000889"
     CDS             complement(18355..18660)
                     /locus_tag="ACFB78_000889"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268912.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKKIKSAVNFPADFILLQFNLKRPL"
     gene            complement(18701..20068)
                     /locus_tag="ACFB78_000890"
     CDS             complement(18701..20068)
                     /locus_tag="ACFB78_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC1268913.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVILPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAKLSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(20178..20444)
                     /locus_tag="ACFB78_000891"
     CDS             complement(20178..20444)
                     /locus_tag="ACFB78_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268914.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEAPAAEAPAAEAPAAEAAATEAPAAEAPAAEAPAAEAAK"
     gene            20917..21399
                     /locus_tag="ACFB78_000892"
     CDS             20917..21399
                     /locus_tag="ACFB78_000892"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1268915.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            21486..22298
                     /gene="hpnC"
                     /locus_tag="ACFB78_000893"
     CDS             21486..22298
                     /gene="hpnC"
                     /locus_tag="ACFB78_000893"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC1268916.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(22371..22841)
                     /locus_tag="ACFB78_000894"
     CDS             complement(22371..22841)
                     /locus_tag="ACFB78_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC1268917.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            22932..24773
                     /locus_tag="ACFB78_000895"
     CDS             22932..24773
                     /locus_tag="ACFB78_000895"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC1268918.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         5717 a         5909 c         7030 g         6474 t
ORIGIN      
        1 ttgtgcttgg aagaaatgac ggggcaaacc gtctctgagg gcgcgctgtg gtatatgcaa
       61 acccgccacc gtgtccccgt cgtgttttca gacggcctga gacctttgca ataacatagg
      121 ttactaaaat tttatgctca atctcatttt caaaatgcaa aacttttctg atttttccta
      181 ctttttgctc aatattagga aggttttagg caattgaaaa ttttttggcg catttttatg
      241 cgtcaaattt cgttaacaga ctatttttgc aaaggtctcg gcctacgcgc ccaaaccctc
      301 gcaactatag ccgccgtccg cgaactctta aacagcggac aaaccccgcc gcccgactac
      361 ggcaaacgct gcaaagcctg ctctctggtg gaaatctgtc agccggagtt gttggcgaaa
      421 cgggatggga gtgtgggata tgtggaagcg ttgtttattg tgtaagaaaa aaccgcaggt
      481 atttgaaacc tgcggttttt tggtttacag cttgctgtct tcgctgtcca agaaacttct
      541 caaacggtcg cttcttgtcg gatgccgcag cttgcggagt gcttttgcct cgatttgacg
      601 gatgcgttcg cgcgttacgt caaactgtct gccgacttct tccagcgtgt ggtcggtgtt
      661 catatcgatg ccgaaacgca tacgcaggac ttttgcctca cgcggtgtca ggctttcgag
      721 gatttctttg gttacttcgt gcaggctggt gtacattgcc gcatcggccg gcgcaacatt
      781 gttggcatct tcgatgaagt cgcccaagtg cgaatcgtcg tcgtcgccga tgggggtttc
      841 catcgaaatc ggctctttgg cgattttcat gattttgcgg attttgtctt cgggcatctg
      901 catcagttcg gcaagtttgg cggaatcggg ttcttcgccg gtttcttgaa ggtgttggcg
      961 cgagatgcgg ttcatcttgt tgatggtttc aatcatatgt accggaatac ggatggtacg
     1021 cgcctgatcg gcaatcgagc gggtaattgc ctggcggatc caccaggttg cgtaggtgga
     1081 gaatttgtag ccccggcggt attcgaactt gtcgaccgcc ttcatcaggc cgatgttgcc
     1141 ttcctgaatc aaatcaagga actgcaagcc acggttggtg tattttttgg caatggaaat
     1201 gacgaggcgc aagtttgcct gaatcatttc ctgtttggcg gctgcggttt ctttttcgct
     1261 cgacaccata tttttgttga tttctttcaa ctcttcgatg gaaatgcggg tttctttttc
     1321 catatccgcc aactcggttt gtttttcgag gatggcgtgg cggaagcggt cgagtgcgtt
     1381 gctccaaacc ctgcctttgg cgatttcttc ttcaatccat tgcagattgg tgatttcggg
     1441 caggaagttt tggatgaagt agtcgcgttc catatggacg cggtcgaggc agatgtcgcg
     1501 gatttcgcgt tcgagtttgc ggatgttttc tacttttccg cgcaggctgc tgctgaggct
     1561 gtcgatttgc cgggtggcga aacggacttc cagcagtttg ttggcaatcg cgtcgcggta
     1621 ggcgagatag tctttgtgcc ggctgtggtg tttttccaaa cagccgatca tttttttgta
     1681 gtctttttcg atttgggcaa agtggccgat gactttttgt ttcaattcgg cgagattggc
     1741 tgccgagact tcatccgcat cttcttccga ttcttcgtcg tcttcgtttt cgtccgaatt
     1801 gtcgttggaa ggtttctcgg gtgctgtggt ttccaagtgc cccaagccca attcgttgag
     1861 caatacttca ttcgggtcga taatggcttc tacgacttcg tcgacgcgga tttcgtcttt
     1921 gcagattttt tcgatgagtt ctaagatttc cgcaatggaa cccgggcagg cggagatggc
     1981 ctgaaccata tttttcaggg cgttttcgat ttttttggcg atgatgattt cgtcttcgcg
     2041 ggtcagcagg tcgacctgtc ccatttcgcg catatacata cggacggggt cggtcgtcct
     2101 gccgaactcg gaatctgcgc tggaaagggc ggcctcggct tcttcgacgg catcatcgtc
     2161 ggtaacggcg gcattgtcgc ttaacaatat gtcttccgca tcaggggcgt gttcggtaac
     2221 ttggataccc aaaccggaaa tcatactgac gatgttgtcg atttgatcgg catcggacat
     2281 atcgtcgggc agtgcgtcgt tgatttcgga gtaggtgatg tagccgcgtt ctttgcccat
     2341 aatgatgagt tggcgcaggc gcgcacgttg ttcctcaatc gtcagggggc gggcatcgtc
     2401 ttggtattct tcgtgatttt ggtttttgga cattgtctgc tttctgttgg caagatgccg
     2461 acggtttgcg tttggacgca cctgaaggcg tggcaaagta gggtactctg atgccgtctg
     2521 aaggcttttt gggatgggtg ttctgcaggt gtcgttgttg cagccggggt gaatgctttt
     2581 cagacggcat aacggtttac agatggcgga atccgccggt caattttgtt ttgcggtcag
     2641 cagcgacagc aaaagttttt tctcgctttc atttaagccg gattgcaggc ttttttgttt
     2701 taatgtttca atttggctgt attttaactc attgagcagt tttttgatgc cgatttggaa
     2761 gttttcgcaa tcttcttcgc cgccgccttc catttcttcc gattgaaggg ccgaccggaa
     2821 gatgcggtcg attgtttctt cgtagggcga accgcgcata tgttccaaaa cctgtgcggt
     2881 tgccggcacg gaaggatggt ttttaatggt ttcggcgagg ttggcaaggc aggcgaaatc
     2941 gccgtccaac gccagataat cgggcaggtc tatatatgca gcccaatccg gatttatcaa
     3001 gaggctgcgg atttgccgtt gcaccaatgt cggcataacg ggctgtttga cggaaatcgg
     3061 aggcagtttg tagttttttt gtttgacgtg ccgctttggc gcttcctgtc cgagcagttg
     3121 cgcgaggttg tcggggtcga tgccgaccag ctcgctaagc cgttgtttta acaaataagc
     3181 caatgccggt gcggtaatct gcaccaaaag cggcgaactg gttttcacca attccgcctt
     3241 gccttcctgc gtattgagat gaatgccgtc tgaaaggtgt tcccagaaat attccgacaa
     3301 aggcttgctt tgattcagaa gcgcgtcttc aaattgcgct ttgccgtagg cgcggatgta
     3361 gctgtcgggg tcgtgttctt ccggcaggaa taaaaaatgc agcgatttgt cgtctttcaa
     3421 ctgcggcagc gcgttttcca gcgcgcgcca agccgctttt cgccccgcgc tgtcgccgtc
     3481 gaaacagaaa taaatactgt ccgcctgccg catcaggatt ttgacgtgtt ccgccgtcgt
     3541 cgccgtgccc aaggccgcca caccgtagcc cacgccgaac tgtgccagcg cgaccacgtc
     3601 catatagcct tcgaccacca aaatccgttc tgcctctttt acagcagcac gcccctcata
     3661 caagccgtaa aggtttttcc ccttatcgaa caaaggcgta tcgggcgaat tcaaatattt
     3721 cggtttcgag tcgtccagca ccctgccgcc gaaaccgatg acctgcccgc gcggattgcg
     3781 gatggggaac ataatccgat ggcggaagcg gtcgtaatgc cgcccctcat tgtcaatcac
     3841 catccccgta tccaccaacg cggtattcgg atacggttgg aacacttgcg ccaaaggctg
     3901 ccagccgtcg ggcgcgtagc ccaaaccata atgcgcgatg acttccgcgc tcaagccgcg
     3961 tttgtccaaa taagctttcg cagccggatt gaatttcagc tgctgcgcgt aaaaatcagc
     4021 tgccgccgcc gtcgtttctt ccagcgtctg ctgttttttc ttgcgctcgg cacggacttc
     4081 gggattatcg ttctgcccgc gcactttagg cacaatcata cctacgcggt cggcaaggaa
     4141 ctgaaccgcc tccggaaacg acagtccctg atgttccatc acaaaaccaa tcgccgaacc
     4201 atgcgccccg caactgaaac aatgataaaa ctgcttggtc ggactgaccg aaaacgacgg
     4261 tgttttttcc ttgtggaacg ggcaacacgc catatagttc gccccgcctt ttttcagcgg
     4321 aacctgctcg tcgatgatgc cgacaatatc ggttttggcc agaagctcgt caatgaagtc
     4381 ggatggaatc atgatgtggg gggacgggat gtctgttttc ggtacggacg gtacgaaatc
     4441 gggatttttc ggaatggcgg tcggatgtgg cggttcaggc aaaatgccgt ctgaagccgg
     4501 aaaccggatg ttcagacggc atttgcgttc aaacgcttaa gccaatttgc cgtggcattg
     4561 tttgtatttc aaaccgctgc cgcaggggca ggggtcgttg cggtggacga tttgtccccg
     4621 cgcttccaag gcttcggggc tgaaatccgt cccatcggga ttaaaggctt cggtaaccag
     4681 atcggtttgc gactgaccca aaagttcttc tatatcgggc gattcggaat ggatggactg
     4741 gatgttgccg acgggttgct cttcaaccgc cgcgacaggg ttttgttcga tttgaaccga
     4801 agtaagcagg gaggcgatat ggaatttgat gccgttccac aggtcttgga acatggtaaa
     4861 ggcttcgcgt ttatattcct gcttcggatt tttctgggca tagctgcgca ggtgtatgcc
     4921 ttgtcgcagg tagtccatgg cggcaagatg ttcgcgccat tggttgtcga tggcctgcaa
     4981 catcacgttg cgctcgaaat cagccattgc ctgcttgccg accagttcgg ttttggcggc
     5041 atattcgttt tcgatacgtt cgatcaggcg ctctttgatg tcttgaccgt caatcgcatt
     5101 gtccgccttc agccaggatt ggatgtcttc ctgaagcctg aattcggcag ccaggcggtt
     5161 ttccagtgtc gggatgtccc attgttcttc catgctgtcg ggcggcatat aggtatccac
     5221 gaggtcgctg acggcatcag aacggatttc ctgcatcagg tcgccgatgt ctttgctggt
     5281 cagaatttcg ttgcgctggc tgtaaatgac tttgcgctgt tcgttggcaa cgtcgtcgta
     5341 ttccaaaacc tgtttgcgca tatcgaagtt tctgccttcg actttgcgtt gcgccccttc
     5401 gatttggcgc gtcagcaggt tgtgctcgat ggcaacgccg cgttcggggg cgaggcggtt
     5461 gaggatggcg gcggcgcggt cgagtgcgaa gaggcgcaac agcgggtctt caaacgagag
     5521 gtagaaacgg ctggatccgg ggtcgccctg acggccggaa cgtccgcgca actggttgtc
     5581 gatacggcgg ctttcgtggc gttccgtacc gatgatgtgc aaaccgcctg cttccatcac
     5641 tttatcgtgt tctgcctgcc agccgttttc gagtgcggcg atttgtgcct gtttctcttc
     5701 gtcgctcaag gtttcgtcgg cgcggatggc atcggtttgg tgcttcaggt tgccgcctaa
     5761 aacgatgtcc gtaccgcgtc ccgccatatt ggtggcgacg gtaattgcgc cgactttgcc
     5821 ggcttgggcg acaatcaggg cttcgcgttc gtgttctttg gcgttgagga cgttgtgcgg
     5881 cagtccggct ttttgcagaa ggcgggagac cagttcggag ttttcgatgc tggtcgtacc
     5941 gacgaggacg ggctgcccgc gtttgtggca ttcctcgatg tctttgacga cggcttcgaa
     6001 tttttcttcg gcggaacgga aaatctggtc gttgaagtct ttgcgctgta cggggcggtt
     6061 ggtcgggatg atgacggttt cgaggttgta gatgctttgg aactcgaaag cctcggtatc
     6121 tgccgtaccg gtcataccgg agagcttggt gtacaggcgg aaatagtttt ggaaggtgat
     6181 ggaggcgagg gtttggtttt cgcgtttgat ttccacgcct tctttggctt ctacggcttg
     6241 atgcaggcct tcggaccagc ggcggccgga catcagacgg cctgtaaatt cgtcaacgat
     6301 gacgatttcg ccgtcttgga taacgtaatg ttggtctttg tggaacagcg agtgcgcgcg
     6361 taatgctgcc ataaggtggt gcatcaggga gatatttgcc gccgagtaca gcgagtcgtt
     6421 ttctgccagc agccccattt gggtcaggat ttgttcggcg tgttcgtgac ctgtttcgct
     6481 caggatgacc tgatgtgcct tttcgtcgac ccaatagtcg ccttcgcctt cttctgtctc
     6541 ttgacggacg aggtggggcg gaacggtgtt catgatttgg tagagctgga tgttgtcgtc
     6601 tgcctgaccg gagataatca gcggggtgcg cgcttcgtca atcaagatgg agtccacttc
     6661 atcgacaacg gcaaaattca attcgcgctg cactttgtcg tattggtcgg ttaccatatt
     6721 gtcgcgcagg tagtcgaagc cgaattcgtt gttcgtgccg taggtaatgt cggcagcgta
     6781 ggcgttttga cggtcgaacg gctgcatatc tgaaataatc acgccgacgg tcagaccgag
     6841 gaaattgtag agcggctcca taatgcctgc gtcgcgcgag gcgaggtagt cgttgacggt
     6901 aacgacgtgc acgcctttgc cggccagcgc gttgagatag acggcgaggg tggcgaccag
     6961 ggttttgcct tcgccggtac gcatttcggc gattttgccg tcgtgcagca ccataccgcc
     7021 gataagctgc acgtcgaagt ggcgcatacc gagtacgcgg cgggacgctt cgcggcagac
     7081 ggcgaaggct tcgggcaaaa tgccgtccaa agtctgaccg tcggcgaggc gttgtttgaa
     7141 ttcggcagtt ttggcttgca gatcagcatc gcttagggct tgcatctgtt cttcgagcgc
     7201 gttgattctg gcaacggatt tacggtattg tttcagcaag cggtcgttgc ggctgccgaa
     7261 gattttcttg gcaatgtttg tcagcatgaa taattcctgc gcctgatata atagtggggc
     7321 gaaaacgcga ttttaacaca agcgtttgtg caaagtgcta atgccgtctg aaataaaggc
     7381 gcgtttgcgg tatttcaatc ttttggggtg tatgttttat gaatttggaa cagttgggca
     7441 ggcgggacgc gctgctttcc ggacttttga aacaggcggg acagtggcgg cggttggatg
     7501 ctgccgtgaa aaaactgctg cctgccaacc tgcaccccca ctttcagacg gcatgtatcg
     7561 aagacggcag gcttgtcctt ttggcggcaa acaatatggc ggcatcgcgt ttgaagatga
     7621 ttgccccgtc ggtattgccg cagctggcag ggcttgatgc ttcgatacgg tctgtttcgg
     7681 tcaggctggt tccaaaaccg gaaaaaccgc cgaaaaccaa taccctgcat ttgagtaagg
     7741 ctgcgttgga gagtttcgat tcggcggcgg caaagttgga agaacggcat cccgaattgg
     7801 cggaggcact ggaggagctg gtcaggaagt acggggcata aggcggccgg cagggatgtt
     7861 gtaaatatcc gttaatattt gcgcggcggg ggacggctgc cctaatatta aagaaatagg
     7921 ttcggggtaa gattgccctt tttttgggta aacgattgta aacttgcaaa caggctttga
     7981 tttcaaatga agtttgtagc aaaatgccgc cccgaaacat ctgtttgtgc aacacggcgg
     8041 aatctttttc aaggttttgt taatggcggt tgcactttga tttccgtaaa accgaatatt
     8101 attttatcga ttggagattt accatgaaag cttatctggc tctgatttct gccgccgtta
     8161 tcggtttggc tgcctgctct caagaacctg ccgcgcctgc tgccgaggca actcctgccg
     8221 gtgaagcacc cgcttccgaa gcgcctgccg ccgaagctgc tcctgcagat gctgccgaag
     8281 cccctgctgc cggcaactgt gcggcaactg tcgaatccaa cgacaatatg cagttcaaca
     8341 ccaaagacat ccaagtcagc aaagcatgta aagagttcac catcactctg aaacataccg
     8401 gtacgcaacc caaagccagc atgggtcaca accttgtgat tgccaaagct gaagacatgg
     8461 acggcgtatt taaagacggc gtaggtgctg ccgataccga ctatgtcaaa cctgacgatg
     8521 cgcgcgttgt tgcccacacc aaactgatcg gcggcggcga agagtcttcc ctgactttgg
     8581 atcctgccaa attggctgac ggcgactaca aatttgcctg caccttcccg ggtcacggtg
     8641 ctttgatgaa cggcaaagtg actttggtcg attaatccgc ttaaagtttc aaaagacaga
     8701 cagcctgctt tgtgcaggct gttttattat aaaatgactg cttgaaaagt ccccgttgag
     8761 aacgaaaaca tgaatccgtt tgaaacccaa agcgttacct ttgccgaacc gattgaaatg
     8821 ctgtatgcct gccacggcaa agtgcgccgt ttttgcggcc agattgctat gttgtcgggc
     8881 tatatcgccg aaaacggctg caatcagctt gttttgcaaa ccatccgcca aatctcccgg
     8941 tatttcaacg ttgccgcgcc gctgcaccat gaggacgaag aagaaaactt tttcccgctg
     9001 ctgctgcaat acgcgccgca agcccgtgaa ggcgtggacg agctgttgcg ccaacatatc
     9061 gggctgtacg acaactgggc ggctgtttcc gccgaatttg ccaaactcga agcagacaac
     9121 gcttatatcc ccgatgcgga agcgttcaaa cgctttgtgg agggatatga tgtgcatctg
     9181 gcgattgaag agcctttgtt cgacatgggc aaaacgttta tccccgaaga gaagctgacc
     9241 gaaatcggcg aaatcatggc ggcgcgccgg tgcaaataat gccgtctgaa acaatcgtct
     9301 caaggatata ttatgctgac gcccaaaagc tgcgatttgt tcaatatccc gtttttccag
     9361 tttgcccagc tcaaaaaata ccagcccgag agcattccgc aaatcaaagc cgactacaag
     9421 gaaaactggc agatatggca gcagttgata cagcaggtcg ccgcagattt gagcgaacct
     9481 ttcgccccgc cgcatatcga acgctggtgt aacggctggc aggttcgcgc ccatttcttc
     9541 gcctacttca aatacgccca atacaaaaat tccgccgcca tcctgtcgat actgctcaac
     9601 cgccgccgcc tgagcgtcag cttggactgg cattgctaca aagccgacgt gtcgccgatt
     9661 gcgctgcccg agtacaaccg ctggctggat gatttcgata ccgaaaaata cgccgcattc
     9721 gatatgtggc acggcgcgga aagcgaatat gacgactacc gcaccgtcgc acaacaaagc
     9781 gaatccgacc gaaggttgca aaacgacgaa gactttttct gcatcggcaa acacattgag
     9841 cgcgacgatt tgggcaagca ggatgtagca aaatggatag ccgaaacggt ggaagattta
     9901 ctgccgcttt acgaagcctg tcacggcaaa tgattcagaa aactgacggg tacgaatgcc
     9961 cgtcttatac accaatccaa cccggaggtc gtctgaaaat cagcttcagg acatttcaga
    10021 cgacctgatt tccaaagtga acaaaatgac cgaaacccaa tccctagaac tcgccaaagc
    10081 gttgatttcc cgcccgtccg ttacccccga cgaccgagat tgccaaaaac tgcttgccga
    10141 acgcctgcac aaaatcggtt ttgcggctga agaactccat ttcggcgaca ccaaaaacat
    10201 ctggttgcga cgcggcacga aagctcccgt cgtctgtttt gcagggcata ccgatgttgt
    10261 gccgacaggt cctgttgaaa aatgggattc gcccccgttc gaaccgaccg agcgcgacgg
    10321 aagattatac gggcgcggcg cggcggacat gaaaaccagc atcgcctgtt tcgttaccgc
    10381 ctgcgaacgc tttgttgccg aacatcccga ccatcaaggc agcattgcac tcttgattac
    10441 ttccgacgaa gagggcgatg cactggacgg cacgaccaaa gtcgtcgatg tgttgaaagc
    10501 acgcggagag ttgattgact actgcatcgt cggcgaaccg accgccgtgg acaaattggg
    10561 cgatatgatt aaaaacggcc ggcgcggctc gctgtcgggc agcctgaccg tcaaaggcaa
    10621 gcaagggcat atcgcctatc cgcatttggc ggtgaatccc atacatactt ttgccccggc
    10681 cttgttagag ctgacgcagg aaatttggga cgaaggcaac gaatatttcc cgccgaccag
    10741 ctttcaaatt tccaatatca acggcggcac aggcgcgacc aacgtcattc cgggcgagtt
    10801 gaacgtcaaa ttcaatttcc gcttctccac agagtccacc gaaacagggc tgaaacaacg
    10861 cgtccacgcc attttggaca aacacggcgt gcaatacgat ttgcagtggt cgtgttcggg
    10921 acagcccttc ctgacccacg cgggcaaact gaccgacgtg gcacgcaccg ccattgccga
    10981 aacctgcggc gttgaggccg aattgtccac caccggcggc acttcggacg gacgcttcat
    11041 taaagccatt gcgaaagaac tcatcgaatt aggcccgtcc aatgccacca tccaccaaat
    11101 caacgaaaat gtgcggctgg acgatattcc gaagctgtcg gcggtgtatg agaggatatt
    11161 ggcgcggttg ttggctgaaa aggccgtctg aaaaaaagat gggctggttg tcggatatgg
    11221 atgtccgaca atctgattag acccaacatg agtttgagat aacgcattga tttttatgta
    11281 gagcgattta tttgaacggt aatccattcc ctctcccgtg ggagagggtt agggagaggg
    11341 cattgggacg gtaacggaaa tattgtctgt atctgccacg ttttaccctc cctccgaccc
    11401 tccccacagg ggagggagct aagttgcagg cacaccgacg gaatatggtt gaaaccctgc
    11461 ccttcagggc agtagaattt tggtttattt tttgggaagt aaccccttcc aaatcagggt
    11521 aaacagggct gcgccttatg tgcggccctg tgtgttgaaa cattgaggaa tataaccgtg
    11581 ccgtcaaatt actttcatac gcgtgaacga ttgggtgctt taggcggcat cgtgcaacat
    11641 cataccgcct tgtaccgcag cgtggcggta tatgaaccga ccgtggttat cgtgcggcgc
    11701 ggatgcaaga agctgcggtg ggcggggcgc gaattgagga ttgcggcggg tgaggctgtc
    11761 ggcttggcag gcgggcagac gtttgacgtc atcaatattc ccgattcaga cggcctctat
    11821 caggcgcaat ggattgcttt cgaacaggaa accgtagaaa ggttcgccgc acaatacggg
    11881 acggcgcagg cagtgtgcga tgcggtgaag ctgccgcacc ccgggcgtat gggcgcggcg
    11941 tttgattatg cggctgcggt actggcggat gaagaagtgc cgcacaatgc ggcggaggcc
    12001 gcgctttgcg gcgtgttggc gtggttgcag catgacggca tcggttttgc cgtgtacgga
    12061 ggcgtcaacc tgatgcggca aatccgcaaa ctgattactg ccgatatggc tgcggactgg
    12121 tcgtcggcaa tgctggcgca gcggcttaat tgcagcgagg ccgcgttgcg gcggcggttg
    12181 gcgcggcagg acacgaattt ccgtacgctg ctgacggatg tacgcatgat gcgcgcgctg
    12241 acgctgttgc aggttacgca atggtcggta gcgcagattg ccggcgcggt tggctatgac
    12301 tgcccgtccc gtttcagcgc gcgcttcaaa gagctgttcg gttgcgtacc gtcggtggtc
    12361 cgctcggaag cggagccggc ggcatatcgg cgcacggggc aggtcagcgt cggtgttcga
    12421 ccgtgaattt cgcttcgccc aagctgtttg ccttagtgga gaaaccgacc agcacgggtg
    12481 tggattcggc ggtgatgttc ggcagcttgg cgactttcag cgcggtaagc gtgaattcgt
    12541 agcggtgttt cctgccttcg ggaggacacg cgccgccgta gcccggcgcg ccgaagtccg
    12601 tgcgggtttg cagcgcgcct ttgggcggcc tgcctcattg tgcggtaatg cctgcgggca
    12661 ggcggcggac atcggcggga atgtcggcga ccacccagtg catccggtcc agaccgatcg
    12721 gtgcgtcttt atcgtaaacg gtcaggacga aactttttgt ccctgcgggc gggttttacc
    12781 acgacagcgc gggcgaagca ttgccgcccg aacagccgaa gccgtaaggc gcgctcaaaa
    12841 gctggttttg cgtgaagccg ccgtcttcgg acgggttgtc gaattgcagc gtgaatgcgc
    12901 ccccggcaaa ggcagcggtt gaaaaagcca gcagtacggc ggagaggatg cgggtttgca
    12961 tagatgattc ctttgcttga tgaataagtg gattcattat aggctgtcaa cggcggcagg
    13021 aatgtgccga aacggttgga tgatgtgccg gaatgcgcaa atgcaaaaat cgtggtttca
    13081 gacgaccctt tgaaggccgt ctgaaatttt tcatagggcg acgctttatg tgtcgtcctg
    13141 tgtgttgaaa cattcctttc cctgatgctt tggaaatatt gtatatttag gcattgccgc
    13201 aaccagaccc tcaaggtttc cgccgcagcc tgttcctgta ccggcgtgcg ggaatggcgg
    13261 aaatcgggga tttcgccgtc aaatttatga tagggatacg gagcaaacga atgattaccc
    13321 tgccttcctt aaacaatctg ccgtcaaaat gggatgaaat ccgccattgc ctcgaaaccc
    13381 gggtttttga gtgcggcgtt atgccgtatc ccaatctgac ggaatgcgaa gccgggcagt
    13441 tcgagcggga tttttgggac aatatcggct gcgcgcctga agaatatgtc cgtatccgcc
    13501 gcgccatccg tctgttggaa ttacgttatc ccgacagctt gaacgagctg gtgtgtgccg
    13561 ctattgccac gcctttgggc gagatgttgg cggtgttcgg cgttaaggga ttgtgcctgc
    13621 tggagtttgt cggacagaag tatttggaac aggaaattgc cgccgtccaa aaagccttgc
    13681 gcggacggtt tgtgtttcgg gaagacgggc ggatgcaatt tttgcgtcag gaattggatt
    13741 tgtatttcca atgccgtctg aaaacctttg ccacgccctt ggaaatgatt ggtacggcgt
    13801 ttcaaaaaca ggcgtgggac gtgcttttgg cgattcctta cggcgaaacg cgcagttata
    13861 aggagcaggc gcagcgtttg ggcaacccca aagccgtccg cgccgttgcc gccgccaacg
    13921 ggcagaacaa ggtgtctgtc atgattccct gccaccgcgt catcggcagc gacggcaaac
    13981 tgaccggcta cgcgggcggg ttgaaccgca aacagttttt actggctttg gaacgcggcg
    14041 aagttcagac ggcattgttt taagcgggga aatgcagaac ggctcatcgg gcttaaatat
    14101 ggtgtgatga cggctgtgaa aattcataca acggcaatcc ttttaaggca tcggtcttaa
    14161 tgtctttctg attcatatgc ttgtcgtttt gtcgcccatt tttccaaaat taaggattga
    14221 cacatttttt ccggatgcta taatacgccc cattccccga tagctcagtc ggtagagcga
    14281 cggactgtta atccgcaggt ccctggttcg agcccaggtc ggggagccag aattttgaag
    14341 cccgaacttg aatgttcggg ctttttcaca tttgcacgtc cggcgctcgg aatgcggcga
    14401 gataggtttt ccaacggttt cgcggtaaaa ttctgcctgt ctgatttcgg tttcgtttta
    14461 tgtctatcaa aatcctgatt atttctccca gttggatagg cgactgcgtg atgacccagc
    14521 ccttgttccg ccgtttgaag aaacttcatc ccggttgcac gattgatgtg ttcgcaccga
    14581 agtggtcgat ggcggtgttc gagcgtatgc cggaagtgaa tgagattctt gaaaatccgt
    14641 tcggacacgg tgcgttggag ctgaaacgcc gttggcgggt cggcagggag ttgggacggc
    14701 gcggatacga cagggttatc gtgttgccgg gttctttgaa gtcggcagtc atcgcgctgg
    14761 caacgggcat cggaaaaaga acgggttatg tcggcgaaag ccgttatttt ctgttgaacg
    14821 atatacgcag gctggataag gaacgtctgc ctttgatggt ggaccgatat acggctctcg
    14881 cgcatcagag tcaggaagat tttgacgggc attcgggatt ccccgagttt tccattgatg
    14941 aacggcggcg ggaaatttct attgaaactt tcggtttgaa tcttggaaag cctgttctgg
    15001 ctttttgccc gggtgcggaa ttcgggccgg caaagcgttg gccggcaagg cattttgccg
    15061 agttgggcaa acactattcg gaggcgggtt ggcaggtttg gttgttcggt tcgcaaaaag
    15121 atgatgaaat cgccgaagaa atcaactgcc tttcagacgg catgtgtgtc aatttgtgcg
    15181 gcaaaaccga tttgtcgcag gcaatggatt tgctgtcgtt ggcggacacg gtcgtgtgca
    15241 acgacagcgg gctgatgcat ttggcggctg ctttgggcag gaaggtagtg gcggtttacg
    15301 gttcttccag cccgacgcat acgccgcctt tgagcgacag ggcgaaaatc gtcagcctgc
    15361 acttggaatg ttcgccctgt ttcaaacgtg aatgcccgtt ggggcatacc gactgcctca
    15421 acaggctgta tcccgagaag attgtgcagg cggttgaaga ggcggtatga tggcttctgt
    15481 tttcggattt ataccgtctg acggcggata ttgataggat gtttatggct attgcgaaca
    15541 ataaaaaagc atttcacgat tttttcattg aagaccggat tgaagccggt ttggtcttgg
    15601 aaggctggga agtcaaagca atccgcgccg cgcgcgtaca gcttaaagag agttatatct
    15661 attggaaaaa agatgcgttt tatctggtcg gctgccatat tacggctttg cctacggctt
    15721 cgacacacat caaaccggat gccgtacgtc cgcgcaaact gttattaaaa cagtcggaaa
    15781 tcaataagtt aatcggaaaa accgaacgtg caggttacac catcgtgccg ctggatttgc
    15841 atttttcacg cggaaaaatc aagatggaaa tcggtttggc aaaaggtaag aaacagcacg
    15901 ataaacgaca aagtatgaag gaagccgact ggaaacgcga gaaacagcgg ttgattaagc
    15961 atacgcgctg attgtggaaa caatgcttca gacggtacgg gcaaggctgc cgcctgatgc
    16021 gtaaacgttc tacacctaaa ccagcatccg ctttaatcag cacggcatga tgccccaaac
    16081 cttcgtcaaa ggtttgagca atttgatgat aaattggcat tagccaaggg taaagtgcgg
    16141 tcatttttcc tgaaattttt gtaaataacg agggcaatta tacacatttt caaaccggct
    16201 tgacaaaata gcaaaaagca aacgtttact tttaataaat aagaaataaa tagagaaaat
    16261 ttgaactaaa tgacattttt tgttcagttt tatgctaaaa tccacgccct atttattttt
    16321 acttgacaaa ggaaaattta ttatgaaact cgaagcaagc aagcagaagt ttaaaaaatc
    16381 atttattata agtctatttt tttctattct ttaatacctc tccgcttttg gctgttgatt
    16441 atgtttacga caaaaccaag ctcactgatg atgaaattat ccgcttaaaa aaaactccgc
    16501 gatagaaata gtgaatattg gaaagaagaa acttatcaca taaaaagtaa cgggcgaact
    16561 tatccaaaca ttcccgcatt attccctaaa catcctttcg atccattcga aaacatcaac
    16621 aactcaaaaa agatttcttt ttatgacaag gaatacactg aagattacct tgttggcttc
    16681 gcccgaggtt tcggggttga aaaaagaaat ggggaagaag aaaaaccact ccggcaatat
    16741 tttaaggact gtgtaaatac tgagaattcc aataatgata attgtaaaat ttcatccttc
    16801 ggaaactatg gcccaatatt aataaagagc gatattttcg ccttagcatc acaaataaaa
    16861 aatagccata tcaacagcga aattttatct gtaggtaatt atatcgaatg gcttcgccca
    16921 acacttaata agcttactgg ttggcaagag catctatatg ctggtttaga tccattccac
    16981 tatattgagg ttacagacaa ctcgcacgtt atcggacaaa cgattgattt gggagcatta
    17041 gaattaacaa attctctatg ggaaccccgt tggaactcta atattgatta tttaataaca
    17101 aaaaatgctg aaattcgttt caacactaaa aatgaaagtt tactcgtaaa agaagattat
    17161 gctggcggag ctcgttttcg ttttgcttac gatctaaaag ataaagtccc tgaaatacca
    17221 gttttaactt ttgaaaaaaa tataactggc acatcagata ttatttttga gggaaaagcg
    17281 ttggataatt tgaaacacct agacgggcat caaattgtca aagtaaatga caccgcagat
    17341 aaagatgcct ttagactttc cagtaaatac cgaaagggaa tttatacgct ctctttacaa
    17401 caacgcccag agggcttttt taccaaagtg caagaacgcg atgatattgc gatttatgca
    17461 caacaggctc aagccgccaa taccttattc gccttgcgtt tgaacgacaa aaacagtgat
    17521 attttcgacc gcactttacc acgcaaaggc ttgtggttgc gtgtgattga cggacattcc
    17581 aaccaatggg tgcaaggcaa aacagcacct gtagaaggct atcgaaaagg tgtgcaactc
    17641 ggcggcgagg tgttcacatg gcaaaacgaa agtaatcaac tttctattgg cttaatgggc
    17701 ggacaagcag aacaacgcag cactttccgc aatccggata cagacaatct tacaacggga
    17761 aatgtgaaag gctttggtgc gggcgtttac gccacttggc atcagcttca ggacaaacag
    17821 acaggtgcgt atgttgatag ctggatgcaa tatcaacgtt tccgccaccg cattaacact
    17881 gaatatgcta cagaacgttt tacttcaaaa ggtattactg cctcaattga agcgggttac
    17941 aatgcgttat tggcggaaca cttcactaaa aagggcaaca gccttcgtgt ttacctacaa
    18001 ccacaggcgc aattgactta tttgggggta aacggaaaat tcagcgatag cgaaaatgcc
    18061 caagtgaatt tgcttggctc ccgccaatta caaagccgag tgggcgttca agctaaagcg
    18121 caatttgctt tcactaatgg cgtcactttc caaccatttg ttgctgtcaa ttcaatctac
    18181 caacaaaaac ctttcggtgt ggaaatagac ggggatcgtc gagtgataaa caataagacg
    18241 gtaattgaaa ctcaattagg tgtagcagcg aaaatcaaat ctcacttaac cttgcaagcc
    18301 tcattcaacc gccaaacaag caaacatcat cacgctaaac aaggtgcgtt gaatttacag
    18361 tggacgtttt aagttaaatt gtaacaaaat aaaatcggca gggaaattca ctgccgattt
    18421 tattttttta aaagtgcggt catttttcgt gtaatttcca ccaattttta ccgcactttc
    18481 aatttcagct tgaacaagtt caatagtctg ctctgcatta attacgactg cttttggatt
    18541 atcttttact aattctaaat agcgtgctcg agtgcgatgg aaaaaatcta aatccatttg
    18601 ctcaatacgg tctaactcgc cacgtccacg agctcgcgct aaaccgacaa tcggatctat
    18661 tgtccttttc tttgttgtgt atgcaaatca gaaatgccgg ttacggaagc agtttgccag
    18721 gattcataat gttatatgga tcaaggtgtt gtttgatgct tttcatcagg gcgatttcgg
    18781 caggcgtgcg tactttgtcc agccactgtt ttttgatgat acctatgccg tgttcggcgg
    18841 caatcgtgcc gttgcaggca agaacgttgc gataaaccgc gtcgttgatg tcgttttcgt
    18901 aacgatagac ttcattgctg aggatttcgg gcaggaaagt attgtagtgc aggctgccgt
    18961 cgccaagatg tccgaagcag acgatttgta tgcctttaaa attctgttcc aaatctttgg
    19021 cgcaccggcg gacaaagtcg gcaacgcgcc cgataggaac ggcaatatcg tgtttgatgc
    19081 tggtgcccag tttgcgttgg gaggcggaga tgttttcgcg caacgcccac atatggatac
    19141 gttcttgttc gctttgcgcc aatacgctgt cggtaaatcc ttttttataa agaaattcga
    19201 caagccgatc atcaagattg ctgtcgggta atgagtcggt caactcaagt aaaatatgcc
    19261 attctgaatg tgtcggcagg gggagtttgc tgaattcgga agacaacttg gcggcaaaac
    19321 ggccgatcag ttcaaaactg cataagcgtt cggcaaagtg cgcttgggtt tcggtcagca
    19381 ggcggacggc ggattcgatg tcgggaatgc cgacccatgc cgttgccttg tccgagggac
    19441 gggcaaaaag cttgagcgtg gcggcagtga tgatgcccag tgtaccttcg ctgccgataa
    19501 acagatggcg caggtcgtag ccggtggtgt ttttatgcag gggatggaga tgggaaacca
    19561 gttcgccgtt ggggaggatg acttccaaac cgataaccag gtcgcgcatc gtgccgtaac
    19621 gcaatacgtt caaacctccg gcattgcagg cgatgttgcc gccgatttgg cacgagcctt
    19681 cgctggcgag actgagtgga aacagcctgt ttgaggcttc ggctgcctgt tggacggttt
    19741 ggagtacgga acctgcttcg acggttatgc agttgtctga caaattgatg ctgcggatgc
    19801 ggttgagttt ggaaaggttc agcaatacgc cgttttccga tactgccgcg ccgcacaaac
    19861 cggtattgcc gccttgcggc gtaaccggaa tgcggtgttc gtaacaaaaa cgcataatgg
    19921 tttgcacgct ttcaacgctg cgcggctgca aaatgatgtc tggtgcagac gtaaagcggt
    19981 tgcgctgatc ttttaaaagc gtcggagaag gttcggcaat ttcatctgcc ggcaggagtc
    20041 gggaaaattc ggtatgaagg tcaggcatgt ttgtcggaag ggtagggaga agattgagga
    20101 agggaatctg atgccgtctg aaaaatcaaa aaagcaggat actgaacgta tcctgctttt
    20161 ttgcaagcgg aaaatgctta ttttgcagct tcggcagcag gagcttcagc ggcaggtgct
    20221 tcggcggcag gtgcttcggt agcagcagct tcggcggcag gtgcttcagc agcgggagct
    20281 tcagcagcgg gagcttcagc agcgggagct tcggcagcgg gagcttcggc ggcaggtgcc
    20341 tcggtagagg aagcctcagc agcaggagct tcagcggctt tttctccgcc gcaggcagcc
    20401 aaagccaaag acaacaaagc agcggcaaac agggattttt tcattttagt aacctttaaa
    20461 atcaatttaa attattgaaa caaataggtc tttttcgacc atgctacaaa ccgggttgga
    20521 aattcatcgc tgaatttgcg taattatgca ccatggaata agggattgca atgtcgtaca
    20581 gatgggaaaa aggtttgaat gaaaagtttt ctggcgggcg gattgataaa tcctgtaaaa
    20641 cggttttgct gttaaaaatc cgatagggat aaatattata gagattaata taattcagta
    20701 tcttgtatga tttattaagg cagattgggg aagtcggcag atgggtcgga atggtttttg
    20761 agagtgttgt aaaaaattta cactgcaata aaataacaaa aatttacatt gaaatatgtg
    20821 ccgtacagtt tatgaacagg ttaacggtaa tcggccggcg cggcggttgt gttagaatgc
    20881 gcctgtaatc aatctgctgt tcctgaaagg acaaatatgg ctattgcaaa aaattccgtg
    20941 gtttcgctgc attatgagat gtatgatgcc aacaatcagc ttttggataa aaccgaagaa
    21001 ccgattgcgt atctgcacgg cggttacgac ggcattttcc ctttggtgga agaggcgttg
    21061 cacggtaagg atgccggcga tacggtcgat gtggcgcttt cgtccgacga tgcgttcggc
    21121 gagcaggatt cggagttggt ccgtattgaa gatgcgggcg cgttccctgt tgaagtcgaa
    21181 gtcggcatga tgtttgaagc cgacgatcct gaaaccggcg atgttgtcgt ctatcgtgta
    21241 accgatgttg ccgacggcaa ggcggtggtg gacggcaacc atcctttggc aggcatgaaa
    21301 atccgcttta aagctacggt tgaaagcgtg cgcgatgcat ccgatgagga aatcgcacac
    21361 ggtcatgtcc acggttcgca cggtcattac caccactgat tgccggcagc ttggaggtat
    21421 ctgatgccgt ctgaaaaggg tttgttccgt ttcagacggc attttgtttg atggatggag
    21481 aaggtatgtc ggttgggcat tatgagaatt ttcccgtcgg ttcgctggtt ttgccgcgca
    21541 ggttgaggaa gccggttcat gcggtgtatg cgtttgcacg gacggcggac gatatggcgg
    21601 acgagggcag tatgccgtct gaagccaggt tgtcggggtt ggagggtttg cggcgcgagt
    21661 tggacgtgtt ggcatcgggc ggccggtcgg cgcatccttt gattgcacga ttggatgccg
    21721 aggcggttgt gccgttcggt ttggatttgc agccgtttta tgatctgctc tcggcgtttt
    21781 cgcaggatgt ggttaaaacg cggtacgcgc atttcggcga tctgaccgat tattgccggc
    21841 gttccgccaa ccctgtcgga cgcattatgc tggctttata cgggaaaacg gatgcggtgt
    21901 gcgtagcgca aagcgacggt atttgtacgg ctttgcaact ggtgaatttt tggcaggatg
    21961 tagctgtgga ttggcaaaag ggcagggttt atatcccgca ggacgatttg ttgaaattcg
    22021 gtgtttctga ggaacagatc gcagcaggac gggcggatgc ggcgtttcag cggctgatgg
    22081 cgtatgagtg ccggcgtgca ttccgtatgc tgaaggcggg ttcgcctttg gcgcgcgaat
    22141 tgaaagggcg tatcggtttg gaactccgta tgattgtgtt gggggcgcag ttgattttgc
    22201 agaaactgga cgcgtgccga tacgatgtgt ttgcacaacg ccccgttttg gataagaagg
    22261 attggttgat tatgttgaaa cgcgcgttgt ggaaatgatt gggggaaaat gaatgccgtc
    22321 tgaaaggggt tgttccgctt cagacggcat tttgcaggcg gtcggtatgg tcaggcgatt
    22381 tcatgcgggt ttaacctggc ttgatcctct gcacccaagc cgatgagttt gcgcagtcgg
    22441 gtcatgtaga aattgacatc caaaccggtg ccgtagcgtt gcgccgtcca cagtgtttcc
    22501 gccagtgcct ccatcatttc gtgttcggct tccggccagc cgcgtttggc gcacagggtg
    22561 tcgtggatgg cgcgtatgcc gtgcggctgg tctatgcccg cctgttcttg gacggacaaa
    22621 tgcagcgaca tatgcaggaa ggggttgctt tcgccgtttt cgggcagcca gtcggtgtcc
    22681 agatggtctt cgatgcgttc gagataacgg tggtattcgg gatgggcttc gacaatgcgg
    22741 agggctttct gttccaacgc gcccaattgc agcggattga gccgctgctg ccacacgcgg
    22801 gcgaaaaagc ggcggacatc gtgggtattg acatcgtaca tggcgggtcc gataagatgt
    22861 ttatggaaat aggttcggat aatggcgggt tttacataaa ttaaatccta ccgtcaacct
    22921 taccatttat aatgcggaca aattatttgt ctgaaacgga ttcgattatg aaaaaactga
    22981 tttgcctgtt cgccgtattt ttgatgttgt gcggacgggc tttcgcgctg gatgcgaacg
    23041 atctgctgcc gccggaaaag gcattcgtgc cggagcttac tgttgccgac gacggcgtga
    23101 acgtccgttt caggattgcc gacggatact atatgtatca ggcgaaaatc gtcggcaaga
    23161 ccgatccggc ggatttgttg ggacagcctt ctttcagtaa gggcgaagag aaggaagacg
    23221 agtttttcgg caggcagacg gttcaccatc acgaggcgca ggttgccttt ccttatgcaa
    23281 aggctgtcgg cgaaccgtat aaattggttt tgacctatca gggctgtgcc gaagccggcg
    23341 tgtgctatcc gcccgtggat accgagtttg atatttccgg caacggcact taccatccgc
    23401 aaaccgacga accggcatcc gccaaagacc gttttttgca gccttcctct caaaacggca
    23461 gcggggcgtt gccgcccccg aagggggacg agggcggcga cggccgtttc aagctgtctt
    23521 gggatacgct caacgccaat cttttggcgt tttttctcgc cggtttgggc ctgagtttta
    23581 ccgcttgtat gtatcccctg ctgccgattg tgtccagtat cgtggtcggt gacaaaaagg
    23641 cgggcaaggc gcgggcgttt gtgctgtccg tcgtttatgt tcagggtttg gctctgactt
    23701 atacgctggt cggcattgtt gccggactga cgggcgcgct gctgaccgta tggttgcagc
    23761 aggcttgggt cgtgttggcg gcatcggctt tgatggtcgt cttggcgctg tctatgttcg
    23821 ggctgttcaa catccagctt cccaacgccg tacagtcata tttccaaaac cagagcagca
    23881 ggctctccgg cggtaaaatc gtttccgtct ttattatggg cattctgtcc gcactgattg
    23941 tcggaccgtg cgtcgccccg ccgctggcgt ttgccttggg ctatatcggt cagacgggcg
    24001 atgcggtttt gggcggtttg gcactttaca ctttggcgtt gggcaccggc gtgccgctga
    24061 ttgccatcgg cacgttcgga ggacatatcc tgcctaaggc gggcgattgg atgaatgccg
    24121 tcaaatacgc tttcggcttt atcctgcttg ccgtcgccgt ttacctcgcc acgccgcatt
    24181 tgccctatta tctcgtcgtc gcgctgtaca cgctgctgat gctggttcct gcctttatgc
    24241 tgctggtcaa cggacgcagg cagaaacgcc gtccgaaagc tgtggcattc gcattgggca
    24301 gcatattgct gataggcggc gcgtggttcg gctggcaggg cgcaaacggc aaaacgaccg
    24361 cgctgcacca tttcttgacc ctcaatccgc cggccgaagc aggcaaattt tcggaacacg
    24421 gcaaaatgtt tgccgatact gccgcgctga aggcagcgat ggatacggcg ttgaaagaac
    24481 atcccgacaa acctgtcgtt ttggattttt atgccgactg gtgcatttcc tgcaaagaaa
    24541 tggcggttta cacgctcaat cagccggaag tgcatcaggc agtcgatatg gaacgctttt
    24601 tccagatcga cgtaaccgcc aacacgcccg aacatcaggc gttgttgaaa gaatacggtc
    24661 tgttcgggcc gccgggcgtg tttgtcgtcc gcgccgacgg cagccgcagc gagccgctgc
    24721 tgggttttgt caaagcagac aagtttatcg agtggtatga acaaaaccgc tgattccgat
    24781 gtttcaaatg ccgtctgaag ttctcagacg gcatttttgt cagacaggaa aacggcaata
    24841 tccgctatac acacggaagc agtccaaaga tgtgaaaacc gtgtgtataa gtcggtttgc
    24901 gtattgaaat taaaagataa aaatacgatg attaaaaaat aggcataaga aaagataaac
    24961 acagccggtg tttgccggtg tgtgtatctt ttgtggaaaa acatcttaat gccatgtatt
    25021 ttatggaaaa taataacatg attaaaaaat agtcaatcat taaggttcag gtaggcggct
    25081 gcaaccgtca atgtctgaaa agaggtagtg ctgtttaagc atggaaagcc
//
LOCUS       DAXDZG010000025        24977 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXDZG010000025 DAXDZG010000000
VERSION     DAXDZG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24977
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.025"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(59..253)
                     /locus_tag="ACFB78_000896"
     CDS             complement(59..253)
                     /locus_tag="ACFB78_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC1268919.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFB78_000897"
     CDS             complement(243..2324)
                     /locus_tag="ACFB78_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC1268920.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="ACFB78_000898"
     CDS             2847..3227
                     /locus_tag="ACFB78_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC1268921.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3851)
                     /locus_tag="ACFB78_000899"
                     /pseudo
     CDS             complement(3363..3851)
                     /locus_tag="ACFB78_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4183..5616
                     /locus_tag="ACFB78_000900"
     CDS             4183..5616
                     /locus_tag="ACFB78_000900"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC1268922.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEISAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNP
                     TVRKISFTGSTEVGAKIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYVQSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCIDAG"
     gene            5727..6512
                     /locus_tag="ACFB78_000901"
     CDS             5727..6512
                     /locus_tag="ACFB78_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC1268923.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6880..8436
                     /locus_tag="ACFB78_000902"
     CDS             6880..8436
                     /locus_tag="ACFB78_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC1268924.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARITGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFAVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8453..9199
                     /gene="surE"
                     /locus_tag="ACFB78_000903"
     CDS             8453..9199
                     /gene="surE"
                     /locus_tag="ACFB78_000903"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC1268925.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFYYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9301..10314
                     /locus_tag="ACFB78_000904"
     CDS             9301..10314
                     /locus_tag="ACFB78_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC1268926.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLREYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10712..11194)
                     /locus_tag="ACFB78_000905"
     CDS             complement(10712..11194)
                     /locus_tag="ACFB78_000905"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC1268927.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11347..11508)
                     /locus_tag="ACFB78_000906"
     CDS             complement(11347..11508)
                     /locus_tag="ACFB78_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268928.1"
                     /translation="MSEQPEKHHASPIEDERKNPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11582..12043)
                     /gene="recX"
                     /locus_tag="ACFB78_000907"
     CDS             complement(11582..12043)
                     /gene="recX"
                     /locus_tag="ACFB78_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC1268929.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12116..12826)
                     /locus_tag="ACFB78_000908"
     CDS             complement(12116..12826)
                     /locus_tag="ACFB78_000908"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC1268930.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13221..14440
                     /locus_tag="ACFB78_000909"
                     /pseudo
     CDS             13221..14440
                     /locus_tag="ACFB78_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14500..15306)
                     /locus_tag="ACFB78_000910"
     CDS             complement(14500..15306)
                     /locus_tag="ACFB78_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC1268931.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15498..15707
                     /locus_tag="ACFB78_000911"
     CDS             15498..15707
                     /locus_tag="ACFB78_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC1268932.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15796..17010
                     /locus_tag="ACFB78_000912"
     CDS             15796..17010
                     /locus_tag="ACFB78_000912"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC1268933.1"
                     /translation="MDKFPKSAKLDHVCYDIRGTVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17082..19655)
                     /gene="clpB"
                     /locus_tag="ACFB78_000913"
     CDS             complement(17082..19655)
                     /gene="clpB"
                     /locus_tag="ACFB78_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC1268934.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRIKMELDSKPEQMDKLDRRIIQLKMEKMHVAKESDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAASSSTADIKKQMDDIKVKIEQAKRQGDFARASELEYGELPK
                     LGAQLQAAESNPDGKKQNKLFRTEVGADEVAEIVSRMTGIPVSKMMEGERDKLLKMEE
                     VLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAGFL
                     FDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDEVE
                     KAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAVKE
                     VVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTVSD
                     AALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRLKF
                     A"
     gene            19915..20925
                     /gene="trpS"
                     /locus_tag="ACFB78_000914"
     CDS             19915..20925
                     /gene="trpS"
                     /locus_tag="ACFB78_000914"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC1268935.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            21006..21551
                     /locus_tag="ACFB78_000915"
     CDS             21006..21551
                     /locus_tag="ACFB78_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268936.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21868..22212
                     /locus_tag="ACFB78_000916"
     CDS             21868..22212
                     /locus_tag="ACFB78_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268937.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22422..23930)
                     /locus_tag="ACFB78_000917"
     CDS             complement(22422..23930)
                     /locus_tag="ACFB78_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC1268938.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24093..24929)
                     /locus_tag="ACFB78_000918"
     CDS             complement(24093..24929)
                     /locus_tag="ACFB78_000918"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1268939.1"
                     /translation="MNNASSEKTIEFAGRSWTKTADSLPQKAHGLRYGLGVSREINAG
                     GHHFLYGGISRGVHYWDNKDFSEQSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYN
                     FAGGFNADFSRRLGERWRLTLNAGNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDR
                     LLYGGADWPHDMTKEAEQASVRKGLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVY
                     RFPRKDHEYQANLSLRHDKISWKGFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKD
                     FK"
BASE COUNT         5699 a         6290 c         6963 g         6025 t
ORIGIN      
        1 ccttattata aaaataccgt ctgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 taggatttta acgcaaatta gcacactgat atcggttttt acttggaatt tggaaaaatt
     2581 tacattactc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata ggttggcaac ccaatcctca
     3601 tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttccgaact gtcataaacg
     3661 aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3781 atgtttgaac aatcagagct tcttcattag cataaaaaac tgcccaataa ttgatcctgt
     3841 ttaaaaatca ttatttaatc tccgtaattt tgactgtaat gttttgactt ttgccatact
     3901 ctaccacgcg ttgtaactgc aatctttgct tcttatatat tgtaccctat caaagggctg
     3961 cattactttt ctttctaaag attactatcg cataaaaagt atttttctta aacgacaggg
     4021 atgcgcccgt gccatattca aacaccgccc ggatgttgcg ctgcccgatc ggatgcttca
     4081 gacggcatcg gaagggtttg cagttttgga atatgaagat gataatgtcc gcgagattga
     4141 cggcatttga aagcggttat cccgtatacc ggaggacacg aaatgaacga atattcccaa
     4201 ttaatcaagc atcccgatat ttccctttcc ccgatttcag acggcatcgg ggtcggcaat
     4261 ccggcgacgg gcgaaatttc ggcgtatgtc cgcaacacgg gttcggacaa gctgaaaaac
     4321 ctgattcaaa aggcggcggc agcgcaaaaa ttatgggcgg cgaaaaccgc cttggaacgc
     4381 gccgacatat tgtggcgttg gtatttttta gttaaagaaa acaaagaagc actggcgcgt
     4441 ctcatgacga tggagcaggg caaaagcctg accgaggcgc gcggcgaaat cgattatgcg
     4501 gcttcgtttg tgcgctggtt tgccgaagag gcgcggcgga ttgacggcga tgtgctgacg
     4561 agtgtgaaag cgtcgcaaaa actggttgtg ttgaaacagc ccgtcggcgt taccgctgcg
     4621 attacgccgt ggaatttccc gtccgcgatg attgcgcgca aggccgcgcc tgctttggcg
     4681 gtgggctgcg cgatgatagt caaacctgca tcgctcacgc ctttgagtgc gtatgcgttg
     4741 gctgtactgg cctacgaagc gggcgtgccg caggatttgc ttcccgtcgt cagcggccgc
     4801 gcttcggaaa tcagccatga atttgccacg aacccgaccg tgcgcaaaat cagcttcacc
     4861 ggctcgaccg aagtcggcgc gaaaattttt gccggcagcg cggcggacat taaaaaactc
     4921 agtttggaac tgggcggcaa cgcgccgttt atcgtgtttg acgatgccga tttggacaaa
     4981 gccgtcgaag gcgcgctggc gagcaagttc cgcaacagcg gtcagacctg cgtctgcacc
     5041 aaccgcgttt acgtccaatc gggcatttac gacgtatttt gccgcaaatt gagtgaaaag
     5101 gcagccgcgc tcaaattggg cgacggcttg gacgaaggcg tgaaccaagg accgctgatt
     5161 gaggaaaaag cggtagaaaa agtcgaacag cacatcgccg acgcactctc caaaggcgcg
     5221 gtctgtctga ccggcggcaa acgcagcgcg ttgggcggaa cgttttttga gccgactgtc
     5281 ttaagcggcg ttacggcgca aatggcggta gctcgcgagg aaaccttcgg gcctttgtgt
     5341 ccggtgttcc gttttgaaac cgaagccgaa gtcatcgagg ctgcgaacaa tacggaatac
     5401 ggtttggcag cttacctttt cacctccgac accgcccgtc aatggcgcgt cggcgaagtc
     5461 ttggaatacg gcatggtcgg catcaatacg ggcttaatca gtaatgaagc ggcaccgttc
     5521 ggcggggtga aacgcagcgg tttgggacgt gaaggcagca aatacggtgc ggacgaatat
     5581 ctagaattga aatatttgtg cattgatgcc gggtgacgga tgccgtctga atggcagggt
     5641 ttcagacggt gtcgcatttt aagcagtctc tatctgttgt acaatgcgcc ctgtttttac
     5701 ggttattttt gatttgaatg taagatatga tggaaaacgg aaaaacattc ccgagatggc
     5761 gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag cctattggtt gcagggctgg
     5821 cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg acgggcggtc
     5881 tgtcgcttta tcagctggtg gtggcggtcg atatcgtatg cggcccgctg ctgactttaa
     5941 ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc atggtcggca
     6001 tcatccagct ggcggctttg gcgtacggtt tgcacagcgt ttcgctggcg cgtcccgtgg
     6061 tggaagcgtt tgaacaggac cgcatgacca ttgttacggc ggcggaagtc gtggtcgaag
     6121 atttgcacaa agcccccgaa gggctgcaaa gcctgtcgtg gttcggcatc cgccgcattg
     6181 cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg tccctgaaag
     6241 gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag gaagcagaaa
     6301 aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa acgacggttg
     6361 cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac ctgccgttta
     6421 ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac accaaaggct
     6481 acgcgccgat agacggcttc atcatccctt aaccattggg attccgcccg cactcgaaca
     6541 tccgttcttc gcggcggtag aatcagactg tatttgagag gggcgcaacc cctcaatcag
     6601 ggcaacacat agggagacgc tttatgtgcc gtccgtgtat tgaaacattt caaaataagc
     6661 ccatgccgtc cgaacatcct ttcagacggc atggcgtttt gccattgccc cgataagctg
     6721 ttaaactgtt taaattattt cgaccgaagg tacacgcaca tgaaagaccc cgagcagagc
     6781 agtaagcccg cccgccgttt tttgtgcgta ccgtctgttc aggcagcttg agcgtttcag
     6841 gctgcctttt tgcgtttgaa cttgaggaac acgaaaatca tggatttcag ttggttggca
     6901 gaaccgcata cctggatagg ttttgccacg cttttggtgt tggaagtcgt attggggata
     6961 gacaaccttg tctttgtggc gattttggca aacaaggtcc agcccgcacg gcgcgaccgc
     7021 gcacggatta ccgggctggg gctggcagtc gtcatccgca tcattatgct tgcttttatg
     7081 gcgcatatca tcacgctgac cgaaccgctg ttccaaatcg gcggcctcgc cgtttccggc
     7141 aaggacatga ttatgctcgc gggcggtatt ttcctgcttt acaaagccac taccgaactg
     7201 cacgaacgcc tcgaagggca taaccggttt gccgttgccg acaaccaaaa aaaacacgcg
     7261 ccgttttggg gcgtggtcgc gcaaatcctg atactggatg ccgtgttttc catcgattcg
     7321 gtcatcaccg ccgtggcgat ggtcgatcac atcgtcgtgg cgatgggcgc ggtcgtcgtc
     7381 gcaatggctg tcatgatttc cgccagcaaa ctcttgaccg aatttgtgga caggcatccc
     7441 accgtcgtga tgctctgcct tggttttttg ttgatgatcg gtttcagcct gattgcggaa
     7501 gccttccatt tccacattcc caaaggctac ctctacgccg ccatcggctt ctcgatttta
     7561 atcgaactgt ttaaccagat ttcgcaacgc aacagccgca aaaacgacta catcggcagc
     7621 tcgtggcgca agcgcaccgc cgaaaacgtt ttgggtatga tgggcatacg cgaaagcgtg
     7681 cttgctgacg cgggcggcga atccgtggat gacgcgcatt ttgaagaaaa cgaaaaatcg
     7741 atgatacgca gcgtgctgac gcttgccgaa cgcccgatta tgggggtgat gattccacgc
     7801 cgcgacatcg aacggctgga catttcccaa agccgcgaag aacagtatgc ccaactgcaa
     7861 aacacgcctt acagccgcct gctcgttgtc ggaaaggcgg gcgtggacga accgttgggc
     7921 tacatcaaca aaaaagacct gctgtcccaa ctgctggaaa caggcggtct cgacattcag
     7981 acggcattgc gccagccgct cgtcctgccc gacggcacga ccgccttggg cgcgctcgaa
     8041 ctcttccgcc aaagcagcgc ggattacgct ttggtggtgg acgagttcgg cgcggtgttg
     8101 ggtatggtga cgatgaaaga cctgttggaa gccatcgcag gcgagttccc cgaagaattc
     8161 gagcgcgaag aagaacccgc cgttcagggg aatcccgatg aaagcctgac ggtggaaggc
     8221 gcgttggaat atgtggagct ggcatcccaa ctcaacctgc cgcagcagga ggaagatacc
     8281 gatttccata ccgttgccgg gctgattatg gaagaattgc aaaccatccc cgatgtcggc
     8341 gattttgccg attttcacgg ctggcggttt gaagtggtcg aaaaagaagg gcagcgcatc
     8401 gagcgggtca aaatcaccaa attgcccgaa gaataagcat ccaggataaa aaatgaacgt
     8461 tttgatttcc aacgacgacg gctatctggc agaaggcatt gccattctgg cgcgggttgc
     8521 gtcggaattt gccaatgtca gggtggtcgc gcccgagcgg gacaggagcg gggtcagcaa
     8581 ttcgctgacg ctcgaccgtc ctttgcagct gaaacaggcg caaaacggct tctactacgt
     8641 caacggcacg ccgaccgact gcatccatgt cggccagttt gccctgcccg attttaagcc
     8701 cgatgtcgtg ttttcaggta tcaaccgtgg cgcaaatatg ggcgacgata cgctttattc
     8761 ggggacggtt gcggcggcga ccgaagccta tctgatgggt atgcctgctg tggcgttttc
     8821 tttaaacgat gcttccggac gctattgggc aaccgcagaa aaggcactgt ggacattgtt
     8881 ggcgcatttt tttaaaaaac cgccgtccgc gcccgtcttg tggaatgtca acatccctgc
     8941 tgtcgcaccg gaagatgtca ggggaatcaa gattacccgt ttgggcagac ggcatcatga
     9001 gcagaacatc gtcccgtccc gcaatccgcg cggcgaacag atttattgga tagggccggt
     9061 cggcgaagtt tccgatcggg aagaggggac ggattttggt gaatgcggcg caggttttat
     9121 taccgtaacg ccgctgcaaa tcgatttgac cgcctatccg gacatggcgg agacggcggc
     9181 cttttggcat acggactgac cgtttttcaa ataccgtcgg gttttgcgct tgagaagaac
     9241 gcgcattttt attatgatat accgcattca ggctatatat cggaacatga aggttatctt
     9301 atgttgaaac aaacgacact tttggcagct tgtaccgccg ttgccgctct gttgggcggt
     9361 tgcgccaccc aacagcctgc tcctgtcatt gcaggcaatt caggtatgca gaccgtatcg
     9421 tctgcgccgg tttacaatcc ttatggcgca acgccgtaca atgccgctcc tgccgccaac
     9481 gatgcgccgt atgtgccgcc cgtgcaaact gcgccggttt attcgcctcc tgcttatgtt
     9541 ccgccgtctg cacctgccgt ttcgggtaca tatgttcctt cttacgcacc cgtcgacatc
     9601 aacgcggcga cgcatactat tgtgcgtggc gacacggtgt acaacatttc caaacgctac
     9661 catatctctc aagacgattt ccgtgcgtgg aacggcatga ccgacaatac gttgagcatc
     9721 ggtcagattg ttaaagtcaa accggcagga tatgccgcac cgaaaaccgc agccgtagaa
     9781 agcaggcccg ccgtaccggc tgccgcgcaa acccctgtga aacccgccgc gcaaccgccc
     9841 gttcagtccg cgccgcaacc tgccgcgccc gctgcggaaa ataaagcggt tcccgccccc
     9901 gcgcccgccc cgcaatctcc tgccgcttcg ccttccggca cgcgttcggt cggcggcatt
     9961 gtttggcagc gtccgaccca aggtaaagtg gttgccgatt tcggcggcgg caacaagggt
    10021 gtcgatattg ccggcaatgc cggacaaccc gttttggcgg cggctgacgg caaagtggtt
    10081 tatgccggtt caggtttgag ggaatacgga aacttggtca tcatccagca caattcctct
    10141 ttcctgaccg cgtacgggca caaccaaaaa ttgctggtcg gcgaaggtca gcaggtcaaa
    10201 cgcggtcagc aggttgcttt gatgggcaat accgatgctt ccagaacgca gcttcatttc
    10261 gaggtgcgtc aaaacggcaa accggttaac ccgaacagct atatcgcgtt ctgactttcc
    10321 gtttgcaatc aggttaaaaa tgccgtctga aaggtcttca gacggcattt ttgttttggc
    10381 ggctgtgttt tcaaatcatt ccgaagggac ggggcgtata aatttgtttg actgtgtttg
    10441 ggtatttgct tgatttcatc cgtttgcaat ggaaaagccg cttatttccg ttcggagggc
    10501 ggttttatcg gataaaaggc attttgtccg actgattagg gctgcatcaa gggaatatga
    10561 aaacacaacc gcttagtctc acggctgtgt tccaaaatgg cgttgaaaaa cggatgagcc
    10621 agaatcagcc gattttactt caataaatca aaccattaca ccgaatgtcg gaacaatgct
    10681 tgtcctgtct ttcagacggc ataggccgcc gtcagcagcc gcaggacacg tctccggagg
    10741 cttggaggct gatttctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
    10801 tcaggatttc cagcggaggg acggggacgg gtttgccgtc tttgaccgca accatcgtga
    10861 agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
    10921 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
    10981 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgaaagggta acgcaataat
    11041 tgccgctgta acggctggcg caggaatagg caacctggtc gagcaggagc aggagttcgc
    11101 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
    11161 gttcgtggga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
    11221 tttgaaatgg gtgcgggaaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
    11281 ctatggaggg atagtctgcc gaaatgggcc gccgatgccg tctgaagcct tcagacggca
    11341 ttttgttcag gaaagccaaa aacggaaaac caagaaaaac acgagtgcca gcacgcccgc
    11401 ccaaacggcg agcatgaatc cggcaaccgc ccgtcccata cggtaaaccg ggtttttgcg
    11461 ttcgtcttcg atgggggagg cgtggtgttt ttcgggttgt tcggacattt tgttctcaac
    11521 cggaaaagtt ggaaacgggc gtaaggttac gctgccgcgc aaaaagattt aagaaggaag
    11581 gtcagcagct gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
    11641 cggcatcaaa accgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggttgg
    11701 cggcgggatg tttgaatttt ttacgcaaca cggctatggc ggcttgcttt tcgcttgagc
    11761 ggtcgggaag caggttgcgg ctggtttttt catctatgcc ctgttgcgcc aaagcctgtt
    11821 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcgtagcgca
    11881 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
    11941 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
    12001 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcac
    12061 ggaaagattc agacggtatg ggaaacggat tccgatgccg tctgaacaga ttgttttatt
    12121 cttcgtcttt gtttttctgc ggtcgcaggt tttcgtaaat ttcgggcagc gcgccgataa
    12181 tccggtcggg gcgggtcgta tcgtcttgcg agagcagcgt catatcgccg taaccgaagg
    12241 taacgccgac actcaggcag ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc
    12301 cgaccataag catattggcg gcatcgatac ccaaaacttc ggcggcgtgc cgcagcggca
    12361 gcgggctggg ttttttttcg ggcaggctgt ctccgccgag tatcaggctg aagtagtcgg
    12421 cgagtcccag ttgttttaga agctcggcgg caaggatttc gtttttgttg gtaatgatta
    12481 ccagcggaat gcccaaggat ttgagcaatg ccagcccggc ttcggtttcg ggataggggc
    12541 gggtgaagac gctcaaatgg tcgcggtagt atttcatata ggacacgaaa cctttttccc
    12601 acagttcgga atcggcttcg cggtcgcggt cgttggtgag gacgcggtga accagtttgc
    12661 cgatgccgtc gccgacgtag ctttcgacca ctttggcggg cagcggtttc ataccgagtt
    12721 gttccaacat cgcttctgcg gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt
    12781 ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc attcataatc cggtttcctg
    12841 tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg cttctaatgt atagattatt
    12901 aacaatcgca acaggttttt cgggcggtgt tttcggattt tatggcgggg tctggcaagg
    12961 gtttgaaatc ccggcgatgt tccaattaaa tttttaaaaa ataaaatgat gactatatct
    13021 ttatttaatt ggacatttgc ttttcggatg cgcgctgctt ttttcagacg gatgctgtgc
    13081 ggcttgaaaa ataaaatcat atatagatta ataaaatcgg gcttatgtcg actacatgct
    13141 attacgtttt gtcgggaaat tggttatagt aatgagcgaa atgcacgccc ctgaaaatct
    13201 ttttgcgaaa aggaagcaaa atgtctgatg ccgttgcaaa agaaaccctc aatccgtttg
    13261 aaatcgcgcg aaaacaggtc aaaaccgcct gcgaccggct gaaaaccgat ccggccgttt
    13321 atgaaatcct gaagagtccg acccgcgtgt tggaagtcaa actcgacgac ggcacggtca
    13381 aaacctttac cggttaccgt tcgcgtgcgt tcccatcccg gtgtgaatct ggacgaagtc
    13441 aaggcactgt cgatttggat gaccatcaaa tgctgcgttg caggcattcc ttacggcggc
    13501 ggcaaaggcg gcgttacctt ggatccgcgc gattattccg aggcggaact ggaacgcatc
    13561 gcccgagctt atgccgaagc gattgccccg ctgatcggcg aaaaaaatcg atattcccgc
    13621 ccccgatgtg aacaccaacg gcaaaattat gtcgtggatg gtggatgcct atgaaaacgt
    13681 ggtgaaacat tccgcgccgg gcgtatttac cggcaaaccg gttgagttcg gcggctcttt
    13741 ggcgcgtacc gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ctttggaaaa
    13801 actgggcaaa gatgtgaaag gcgcaaccta cgccattcaa ggtttcggca atgtgggcta
    13861 ccacaccggc tattacgcgc atcaatccgg cgcgaaagtc gttaccgtat ctactgttga
    13921 cgttgctatc tacaatgaaa acggcttgga tatggaagcg ctgtttaaag agttccaaga
    13981 aaaaggcttc atcaccaaca aagccggtta cggcaaagaa attaccaacg ccgaactttt
    14041 ggctttggat atggacgtac tcgccccttg cgcattggaa aaccagctga cttccgaaaa
    14101 cgccggtaaa gtccgcgcga aaatcgtggt tgaaggcgca aacggcccga ctacgcccga
    14161 agccgacgtt atcctgcgtc aaaacggcgt attggtcgtg cccgatattt tggcaaactg
    14221 cggcggcgtg gtcgtttcct atttcgagtg ggtgcaaaac ctgcaaggct attattggga
    14281 gtttgacgaa gttcaggaaa aagaaatcgt cgtcctgcgc cgcgcgttcc gcgatatttg
    14341 gaacttggcg caagagtatg atgtcgattt gcgtaccacg tcttatatga tgagtatccg
    14401 ccgtgttgaa aaagcgatga agctgcgcgg ctggtattga tgtttgaaca aaggatgccg
    14461 tctgaaacca tttcagacgg catttttgtt tggacggaat taatggtgag aatggccgat
    14521 ttggaaggtt aaagttgtgt agttcgcctg tttttggcac aggctttgat cggggaaatc
    14581 ggctttgtat tcgacactcg ctttccaaaa gccttggcgc aaggggatga tgtccacttc
    14641 gcctttgccg tcggtggtgt cggagaaggc ttgggcttcg gttttgtgcg ttttgctgcg
    14701 gtcgctggtg tcgaagccgt caaatgtagc ggtaacggtg gcattgggca gcggttcgcc
    14761 acggaacaga acgcggactt tgaagcgttc gcctacgtga atgttggcgg gattgtccag
    14821 cgggacgatt tccaagtttt gtccgaccgg tttggtgatg atggcggtgt ccgcgctttc
    14881 gtgtcccacg ttgacaatgt ttttaccgaa catacgggtt tgttcgcaat agcttgcgtc
    14941 aggcatttct ttgatgccag cctgtttcca gcctgctttg ttttttgacc ggaaagtagg
    15001 ctgatattcg gcggtaacga ggtagctgcc gtctttgacg ggacggttgc tgcggtattg
    15061 gtagttgtat gtgccgcgtt gaatcatgtt ttccttacct ttttcggtaa ccagctgcat
    15121 cggtttgctg aagatgtgca ggcggtcttt ggcgatgggt tcgagttcgg ggaattcgcc
    15181 gtagcccaag tcggctttaa ggtattcgcc gccgtgcgtg tgggcggttt cgacccagac
    15241 gcggtgggcg tgtgcggatg tggcaaatag tgcggaaacg gcgagcagtg tcaatgtttt
    15301 cttcatggtg cagcctttat atcgtgcggg ttaaaaattt tgttatgcta taacaaaata
    15361 tgtatcgtgt gaagacggat ttgccgcctg aaaactgtct ttttgtatcg atttgaattt
    15421 ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat agcaatattt
    15481 caaaacggag aaatatcatg ccttatgtca atattaaagt aaccggcggc aaggaagcac
    15541 cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaat cgaattgctg gcacgcgtgc
    15601 tgggtaaaaa tcccgaaaca acggttgtcg tgattgacga agtggatacc gataactggg
    15661 atataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa gcctgaaaac
    15721 cccgccctga tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca ttgcccaaaa
    15781 tttacggaac acattatgga taaatttccc aagtctgcaa agctcgacca cgtctgctac
    15841 gacatacgcg ggacggttca caaaaaagcc cttcagttgg aagaggaggg caacaaaatc
    15901 ctcaagctca atatcggcaa ccctgcgccg ttcggctttg aagcacctga tgaaatctta
    15961 gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattcgaa ggggttgtat
    16021 tccgcacgca aagccattgt tcactactat cagaacaaag gtttgcgcga tattacggta
    16081 aacgatgtct atatcggcaa cggcgtgtcc gagctgattg cgatgtctat gcaggcgttg
    16141 ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg gacggcggcg
    16201 gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa cggctggttt
    16261 cccaaccttg ccgatatgga agccaaaatc acgtccaaaa ccaaagccat cgtcgtcatc
    16321 aaccccaata acccgacagg agcggtgtac agcaaggaaa tcctgctgga aattgccgaa
    16381 ctggcgcgca agcatggttt gattattttc gccgacgaga tttacgacaa aatcctttat
    16441 gatggcgcgg ttcactgcca catcgccgcg ctcgcccccg accttttgac ggtaacgttc
    16501 aacggtttgt ccaaagccta ccgtgtagcc gggttccgcc aaggctggat ggtgctaaac
    16561 gggccgaaac atcacgcaaa aggttatatc gagggtttgg atatgctctc gtccatgcgc
    16621 ttgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg ttatcagagc
    16681 attaacgaat tcatcttgcc cggcggacgg cttttggaac agcgcaacag agcgtgggaa
    16741 ctggtcaacc agattcccgg cgtgtcctgc gtcaaaccga tgggcgcgat gtatatgttc
    16801 ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgata tgaaattcgt ttacgatttg
    16861 ctggtgcgcg aaaaagtcct gctggtacag ggaacggggt ttaattggat caaacccgac
    16921 catttccgca ttgttacgct gccttacgtc catcagattg aagaggcgat gggcaggttg
    16981 gcaagattcc tgcaaaccta ccatcagtag gattctgata aaaaatgccg tctggaacgg
    17041 agattcccgt ttcagacggc attttcaaca gcaggaacaa atcaggcaaa tttcagtctg
    17101 tcgccgtcgg cttctacctt gatttcgctt tcgggcgcat agtttccggc aagcagggct
    17161 ttggccagcg ggttttcgat ttccgattgg atcgcgcgtt tgagcggacg tgcgccgtaa
    17221 atcgggtcga aaccggcttt ggcgatgatg tccagagcgg catcggaaac agtcaggcgc
    17281 aggttttgtt tttccaaacg tttttccaaa cctttgagct ggattttcgc aatactgcgg
    17341 atattggcct gatccaagcc atggaacacg accacttcgt cgatgcggtt gatcatttcc
    17401 ggacggaaat gttctttcac atcctccatc acaacttctt tcaccgcttc gtagtcctgc
    17461 gtgcccattt gttggatgtg ctggctgccg atgttggaag tcatcacaat cacggtattt
    17521 ttaaaatcta ccgtcctgcc ttgtccgtcg gtcaaacggc cgtcatccaa tacttgcagc
    17581 aggatgttga atacgtcggg atgggctttt tccacttcgt ccaaaagaat cacgctgtac
    17641 ggtttgcggc ggacttgttc ggtcaggtag ccgccctctt cgtatccgac ataacccgga
    17701 ggcgcgccga ttaagcgggc aacggcgtgt ttttccatgt attcggacat gtcgatgcgg
    17761 atgaggtggt cttcgctgtc gaacaggaag cctgccaaag ctttgcacaa ctcggtctta
    17821 cccacaccgg tcgggcctaa gaacaggaag ctgccgtaag gtttgttcgg atcggcaagg
    17881 ccggagcggc tgcggcggat ggcatcggac acggcgcgca cggcttcgtt ttgaccgact
    17941 acgcggcggt gcaacacttc ttccattttc aacaatttat cgcgttcgcc ttccatcatt
    18001 ttggatacgg gaatgccggt catacgggaa acgatttcgg ccacttcatc cgcgccgact
    18061 tcggtacgga aaagtttgtt ttgttttttg ccgtcggggt tgctttccgc tgcctgcaac
    18121 tgtgcgccca atttcggcaa ctcgccgtat tcgagttcgg acgcgcgggc gaagtcgcct
    18181 tgacgtttgg cttgttcgat tttgactttg atgtcgtcca tttgtttctt gatgtcggca
    18241 gtgctggaag acgcggcttt ttcggctttc cagatttcgt ccaaatcggc gtattctttt
    18301 tgcaggccgt cgatttcttc gtctatcagt tccaaacgtt ttttgctggc atcatcgctt
    18361 tctttggcaa cgtgcatttt ttccattttg agctggatga tgcggcggtc gagtttgtcc
    18421 atctgttcgg gtttgctgtc cagctccatt ttgatgcggc tggcggcttc gtcaatcaaa
    18481 tcaatcgctt tgtcgggcaa aaagcggtcg gtaatgtagc ggtcgctcaa ctccgctgcg
    18541 gcaacgatag cagggtcggt aatatcgatg ccgtggtgga tttcgtaacg ttcctgcaaa
    18601 ccgcgcagga tagcgatggt gtcttccacg ctgggttcgc cgaccaatac tttttggaaa
    18661 cggcgttcga gtgccgcgtc tttttcgatg tattggcggt attcgtccaa agtagtcgca
    18721 ccgatacagt gcagttcgcc ccgcgccaag gccggtttca acatattgcc cgcgtccatc
    18781 gcgccgtcgg ttttgcccgc gccgaccaaa gtatggattt catcgatgaa aatcagggtg
    18841 ttgccgtcgt ctttcgccaa atcgttcaat acgcctttca agcgttcttc aaattcgcca
    18901 cggtatttcg caccggcaat caaagccgcc aaatccaaaa ccagcaggcg tttgttacgc
    18961 agggattcag gtacttcgcc gttgacgatg cgttgcgcca aaccttcgac gatggcggtt
    19021 ttacccacac cgggttcgcc aatcagcacg gggttgtttt tggtgcggcg ttgcagcacc
    19081 tgaatcgcgc ggcggatttc atcatcacga ccgataacgg ggtcgagctt accgtctcgg
    19141 gcgcgttggg tcagatcgag cgtgtatttt ttcaaagcat cgcgttggtc ttcggcattc
    19201 gcatcgttca cgttttgtcc gcctcgtacc gcatcgatcg cggcattgat attttgttcg
    19261 gtcgcaccgg cttctttcaa gattttgccg gccgcatcgt tctgctgcac caaggcgagc
    19321 aggaaaagtt cgctggcgat ataggcatcg ccgcgtttgg ttgccgcttt gtccatcagg
    19381 ttcaacaccg cctgcaattc gcggctgggc agaatctcac caccttgtcc ggacactttc
    19441 ggcaggctgt tcaaatgctg ctgcaaacgc tgtttaacct gcggcacgtt cacgcccgca
    19501 tgagccaaga gcgcggcagc tccgctgttt tggtcgtcaa gcagggcttt cagtacgaaa
    19561 cctgcttcca gatagctgct gtccgcagcc aacgccaaac tctgagcttc tgcaagggct
    19621 tgttggaatt tggcggttaa tttgtcgtat cgcattttcg tttccttttc aaaatgtccg
    19681 ctgtcgaagc ctatatgtgc atgactgtgg ataactcaag tgttgttttc tgtttttcta
    19741 tatttaattc gatatattgt tgaatttaaa gtatatagaa atgtataata atatgtataa
    19801 ctatatcttc ttaatatgga aaagtctgtt gtcggctgga tgtatgtggc aaatcgggta
    19861 taatcggcgc atcttttccc tttcagacgg cattgctgcc gtaaggacat ttttatgagc
    19921 aaaaaacgag ttctgaccgg cgtaaccacc accggcaccc cgcatctggg caactacgtc
    19981 ggcgccatcc gccccgccgt ccgcgcggcg caaaaccccg ataccgaatc cttcctcttc
    20041 ctcgccgatt accacggcat catcaaatgc cacgagcagg agatgattca ccaatccacc
    20101 caagccgttg ccgccacttg gcttgcctgc ggactcgacc ccgagcgcac caccttctac
    20161 cgccaaagcg acattcccga agtgatggag ttgaactgga ttctgacctg catcaccgcc
    20221 aagggtttga tgaaccgcgc ccacgcctac aaagccgccg tgcaggcaaa ttcagaaaac
    20281 aatcaggaag atcccgattt cggcgtggaa atgggtttgt tcagctatcc gattctgatg
    20341 actgccgaca ttttgatgtt caacgccaac gaagtccccg tcgggcgcga ccaaatccaa
    20401 cacgtcgaaa tggcgcgcga cattgccggc cgcttcaacc accgtttcca agaactcttc
    20461 accctgcccg aagtgaaaat cgacgaaaac gtcgaactct tggtcggttt ggacggacgc
    20521 aaaatgtcca aatcctacgg caataccatt ccgctttggg aaaacgacaa aaaaacccaa
    20581 aaatcggtca acaaaatcat taccaacatg aaagagccgg gcgagccgaa acagcccgac
    20641 gaaagccctt tgtttgaaat ctataaagcc ttctccacgc cgtctgaaac ggcggaattt
    20701 acgcaaatgc ttgccgacgg cttggcttgg ggcgaggcga aaaaactttc ggcggcgaaa
    20761 atcaacgccg aactggcaga actgcgcgaa cgctacaacg cattgacctc taatccttcg
    20821 caaatcgaag agattttgca ggcaggcgcg caaaaagcgc gcaaagaagc gcgcgaattg
    20881 ttggataaag tacgcgatgc ggtcggcatc cgcccgttga aataaaccca atgccgtccg
    20941 aacctccgcc tgccacgttt cagacggcat ttcagacggc attttgaaac catcaggagt
    21001 gcggaatgct gaaaatacct tttgccgtgt tgggcggctg cctgctgctt gccgcctgcg
    21061 gcaaatccga aaatacggcg gaacagccgc aaaatgcggc acaaagcgcg ccgaaaccgg
    21121 ttttcaaagt caaatacatc gacaatacgg cgattgccgg tttggctttg ggacaaagta
    21181 gcgaaggcaa aaccaacgac ggcaaaaaac aaatcagtta tccgattaaa ggcttgccgg
    21241 aacaaaacgc cgtccggctg accggaaagc atcccaacga cttggaagcc gtcgtcggca
    21301 aatgtatgga aaccgacgga aaggacgcgc cttcgggctg ggcggaaaac ggcgtgtgcc
    21361 ataccttgtt tgccaaactg gtgggcaata tcgccgaaga cggcggcaaa ctgacggatt
    21421 acctgatttc gcattccgcc ctgcaaccct atcaggcagg caaaagcggc tatgccgccg
    21481 tgcagaacgg acgctatgtg ctggaaatcg acagcgaggg ggcgttttat ttccgccgcc
    21541 gccattattg agttattcgg acatcacgga atatatttgg atttttcaaa ccttgcggaa
    21601 aaagtccgcg ccgccgggaa actcaaaagg aggggatgtg tgttacaatt ttccgaactg
    21661 ttttcataaa atagttttcg gacgtgtttc aatatggcat tgatgccgcc ttgttgttcg
    21721 agaaaaaacc ctttatattt aatataatgg gttttaacta aacgggaaac cgttttctct
    21781 ccggccgatg ggcaaaatca gccgattgat ggaacacggt ccggttttta agcaaaacct
    21841 taatcaacaa ctcaagaagg ataagacatg aaaaaattat tgattgccgc aatgatggcg
    21901 gctgccttgg cagcttgttc gcaagaagcc aaacaggagg ttaaagaagc ggcccaagcc
    21961 gttgagtccg atgttaaaga cactgcggct tctgccgccg agtctgccgc ttctgccgtc
    22021 gaagaagcga aaggccaagt caaagatgct gcggctgatg caaaggcaag tgccgaggaa
    22081 gctgtaactg aagccaaaga cgcggcagcc gaaaccaaag aagcggtaag cgaagcggct
    22141 aaagacactt tgaacaaagc tgccgacgcg gctcaggaag cggcagacaa aatgaaagac
    22201 gccgccaaat aatttgttgc cttggcaaaa atgacgagat gccgcctgcc ggcaacccaa
    22261 atgaaaccgc ctgaagattt tcaggcggtt tttgagttgt atgccccttg ttttttacgc
    22321 ctgcatgacc gtgcagggag gatgtgccgc gcatatcggg atattgcgga cagggaaatg
    22381 ccgtctgaac atcatttaga cggcatttgg tttgtcaagg ttcagacggc ttcaactttg
    22441 aacggcatat tgattttttg ccattggacg ctttcatatt ccaatgcgtc ggcaatcagg
    22501 gggtggcgtt ccagccattt cctgtcaata cgcaggatga agccgcagct ttccgtatcc
    22561 gtacgcaact gcatattttt cgggaaaccc agatcttggc gcgaacggca gaacagggcg
    22621 gcaaggcgca gggacaaaac ggcataccac aacatttcgt tgccgccgat aatgccgccc
    22681 atttttttca tatcgccgcg atgaccgatg accagttggg caagtatggt ctgttccttg
    22741 cgtgaaaaac ccggcatatc ggcgttttcg aggatgtagg cggaatgctt gtgatagccg
    22801 gtgtgggcga tgtccaaacc gatttcatgc agcgcggcgg cgcgtccgag atactgttgc
    22861 cacaaggcaa gttcttgaac cgtaacgttt ttggcatgac agaggctgtc cataaaggtt
    22921 tgcgcggtct cggcggtgcg tttcgcctga ttgaggctga cgtggtagcg gtgttggaac
    22981 tcggcaaccg tttgtccgcg catatcttcg tttaagccgc gcccgatcaa atcgtaaaac
    23041 acgccgtcgc gcagggcggc ttcggttacg gtcatcctgt cgagtttcat ttcctcaaac
    23101 gccgccatca tcacggcaag tccgccggca aaaacttcga tgcgttccgg tttcaggttt
    23161 tcaaatttgg cttttttgac cgaaccggct tcgatgatgc gttcggcgag ggcgcgcatt
    23221 cccttgtagg taatgtccgc ctcttggggc atttcggcgg caagcacgtc gcggatggat
    23281 tttgccgaac ccgatgtgcc gacggcgaaa tcccaacctt cgcgcctcat atttttgctg
    23341 atgcgctgga tttcgttgcg ggcggcggaa atggcggctt ggaagtcttt ggcggtgatt
    23401 ttgttttgga agaagcgcag gctgtaggtt acgcagccca agggcaggct ttcggtaatg
    23461 tcgggattca gcgtcgaacc gatgacaaat tctgtcgaac tgccgccgat gtcgataacc
    23521 agcattttgc ccccgcaggg ggggagggtg tggatcacgc cggtataaat cagccgcgcc
    23581 tcttcgcgcc cggcgatgat ttcgatgggg aaacccagtg ccgcttcggc cttgggaagg
    23641 aaatctgcga tgtttttggc gacgcggaat gtattggttg ccacggcgcg cacctgctcg
    23701 gggcggaagc cgcgcaggcg ttcgccgaat tttgccaggc agtccaaagc ctgttcctgc
    23761 gaggatgtgc tgaggttttt ctgttcgtcc agaccggcgg caaaacggac catctgtttg
    23821 aaggaatcga tgacttttaa ttgtccgttg ttgttttcgc aaatctggag gcggaaactg
    23881 ttggaaccca aatcgacgga ggcgaggacg tttgcgggat tggtggtcat ggcggatacc
    23941 ggtgggggaa aaacgcaatg ttactctgac ggcgcaggcg ttgacaataa atgatgcggc
    24001 tgtttttgat tctgctcacg gatgttgccg acggtatgtt tccgacagca aaaaatgccg
    24061 tctgaaaaac gttcggacgg cattttttgc gcttatttga aatccttttc cacgctcatg
    24121 aaaatctgcg tatttttgcg tgtgtaaaaa cttttcatat tgctgtcgat tttcagatag
    24181 cggaaattga gttgcggcgt aaagcccttc caagagattt tgtcgtgccg caacgacagg
    24241 tttgcctgat attcgtggtc tttgcgcggg aagcggtaca caattgtccc gggtgcatca
    24301 aaagtcctgc gggtatagcg caggtttgcc cgcagcccca agccgccgtc gaacgttttg
    24361 accgcgccga cacgcaaacc cttgcggacg gaagcctgtt ccgcctcttt cgtcatgtcg
    24421 tgcggccagt ccgcaccgcc gtaaagcagc cggtctttcg gcgcggaata catcagcgtc
    24481 gcgcccgcca gcggcatatg gctgccgtat cgggcggcgg tgcggtcttc ctgataatgc
    24541 ttccacatat tgcccgcgtt cagcgtcagc cgccagcgtt cgcccaagcg tcgggagaaa
    24601 tcggcattga agccgccggc gaaattgtat cggctgccgc ccaagaggtt ttgcccgaca
    24661 aacggcacgg tgccgaacga gcgcgttacc gaacggtttt gatggccgaa cgacaggcgc
    24721 aggctctgtt cgctgaaatc tttgttatcc caataatgca cgccgcggct gatgccgccg
    24781 tagaggaaat gatgcccgcc cgcattgatt tcgcgcgaca cgcccaagcc gtagcgcaaa
    24841 ccgtgtgcct tttgcggcag gctgtcggcg gttttcgtcc agcttctgcc cgcaaattca
    24901 atcgtttttt cggacgaagc gttgtttata gcggattaac aaaaatcagg acaaggcggc
    24961 gggccgcagg cagtacg
//
LOCUS       DAXDZG010000026        23358 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXDZG010000026 DAXDZG010000000
VERSION     DAXDZG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.026"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            99..727
                     /locus_tag="ACFB78_000919"
                     /pseudo
     CDS             99..727
                     /locus_tag="ACFB78_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            873..2549
                     /locus_tag="ACFB78_000920"
     CDS             873..2549
                     /locus_tag="ACFB78_000920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC1268940.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASFSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     RCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            2756..3361
                     /locus_tag="ACFB78_000921"
     CDS             2756..3361
                     /locus_tag="ACFB78_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC1268941.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3433..3825
                     /locus_tag="ACFB78_000922"
     CDS             3433..3825
                     /locus_tag="ACFB78_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC1268942.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            3830..3976
                     /locus_tag="ACFB78_000923"
     CDS             3830..3976
                     /locus_tag="ACFB78_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268943.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(4024..4470)
                     /locus_tag="ACFB78_000924"
     CDS             complement(4024..4470)
                     /locus_tag="ACFB78_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC1268944.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(4602..4814)
                     /gene="rpsU"
                     /locus_tag="ACFB78_000925"
     CDS             complement(4602..4814)
                     /gene="rpsU"
                     /locus_tag="ACFB78_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC1268945.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5104..6954)
                     /gene="ltgA"
                     /locus_tag="ACFB78_000926"
     CDS             complement(5104..6954)
                     /gene="ltgA"
                     /locus_tag="ACFB78_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC1268946.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARATLRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQHSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            7331..8068
                     /locus_tag="ACFB78_000927"
     CDS             7331..8068
                     /locus_tag="ACFB78_000927"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC1268947.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            8070..8756
                     /locus_tag="ACFB78_000928"
     CDS             8070..8756
                     /locus_tag="ACFB78_000928"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC1268948.1"
                     /translation="MAGLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            8915..9781
                     /locus_tag="ACFB78_000929"
     CDS             8915..9781
                     /locus_tag="ACFB78_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC1268949.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(9844..10413)
                     /locus_tag="ACFB78_000930"
     CDS             complement(9844..10413)
                     /locus_tag="ACFB78_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC1268950.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            10571..11431
                     /locus_tag="ACFB78_000931"
     CDS             10571..11431
                     /locus_tag="ACFB78_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC1268951.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            12105..12458
                     /locus_tag="ACFB78_000932"
     CDS             12105..12458
                     /locus_tag="ACFB78_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC1268952.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            12448..13314
                     /gene="atpB"
                     /locus_tag="ACFB78_000933"
     CDS             12448..13314
                     /gene="atpB"
                     /locus_tag="ACFB78_000933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC1268953.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            13371..13607
                     /gene="atpE"
                     /locus_tag="ACFB78_000934"
     CDS             13371..13607
                     /gene="atpE"
                     /locus_tag="ACFB78_000934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC1268954.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            13677..14147
                     /locus_tag="ACFB78_000935"
     CDS             13677..14147
                     /locus_tag="ACFB78_000935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC1268955.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            14152..14685
                     /locus_tag="ACFB78_000936"
     CDS             14152..14685
                     /locus_tag="ACFB78_000936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC1268956.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            14696..16243
                     /gene="atpA"
                     /locus_tag="ACFB78_000937"
     CDS             14696..16243
                     /gene="atpA"
                     /locus_tag="ACFB78_000937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC1268957.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            16268..17143
                     /gene="atpG"
                     /locus_tag="ACFB78_000938"
     CDS             16268..17143
                     /gene="atpG"
                     /locus_tag="ACFB78_000938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC1268958.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDKIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKPPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            17181..18578
                     /gene="atpD"
                     /locus_tag="ACFB78_000939"
     CDS             17181..18578
                     /gene="atpD"
                     /locus_tag="ACFB78_000939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC1268959.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            18589..19011
                     /locus_tag="ACFB78_000940"
     CDS             18589..19011
                     /locus_tag="ACFB78_000940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC1268960.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            19183..20079
                     /gene="glyQ"
                     /locus_tag="ACFB78_000941"
     CDS             19183..20079
                     /gene="glyQ"
                     /locus_tag="ACFB78_000941"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC1268961.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            20196..22259
                     /gene="glyS"
                     /locus_tag="ACFB78_000942"
     CDS             20196..22259
                     /gene="glyS"
                     /locus_tag="ACFB78_000942"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC1268962.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEMIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAGQMNAVADI
                     ALLGE"
     gene            22276..23358
                     /locus_tag="ACFB78_000943"
                     /pseudo
     CDS             22276..23358
                     /locus_tag="ACFB78_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         5446 a         6184 c         6153 g         5575 t
ORIGIN      
        1 ggttttgtta atccactata ttttgacggg gttgaagttg acatctacaa agtttaatgt
       61 ttgaatacat taaaaaatat atttgaatgg gatgttttat gattcaaaat gttgttactt
      121 caataatcct gtattctggg acagccgtag acttacttat tatcctaatg ttattttttg
      181 ccaaaaaaaa agcagaaaag acatcattaa catctattta ggacaatttc taggctctgt
      241 tagcctgata ttgctaagtt tgctttttgc atttgtctta gattatattc ctagtaaaga
      301 gattttaggt ttgctcggct tgattccgat tttcctaggc ctcaaagttt tgctgttagg
      361 agattccgat ggagagtcta ttgccaaaga gggtttgcgc aaagataata aaaacctgat
      421 ttttctagtc gctatgatta cttttgcaag ttgtggcgct gacaatattg gtgtctttgt
      481 cccatatttt actaccttaa atttagcgaa tttgatagtg gctttactta cctttctagt
      541 tatgatttat ctcttggttt tttctgccca aaaattggca caagtccctt ctgtcggaga
      601 aactttggaa aaatatagca gatggtttgt tgccgttgtt tatttaggat tggggatata
      661 tatcctgatt gaaaacaaca gttttgatat gctatggact gtgtcgggcc aggaaaaaat
      721 attatgaaaa agatagtatc tgcaaagact gccccgtgca gtcttttttg ttgccggtct
      781 ttttgtgtct gatgccgtct gaagcagtct ctgcacgacc tttgtgcgaa tatttgctac
      841 acttggcaat ccgtccattt cttctcacgc ttatgctgac ctataccccg cccgatgccc
      901 gcccgcccgc caaaacccac gaaaaaccgt ggctgctgct gttgatggcg tttgcctggc
      961 tgtggcccgg cgtgttttcc cacgatttgt ggaatcctgc cgaacctgcc gtctataccg
     1021 ccgtcgaagc actggcaggc agccccaccc ccttggttgc ccatctgttc ggtcaaaccg
     1081 atttcggcat accgcccgtg tatctttggg ttgccgccgc attcaaacat ttgctgtcgc
     1141 cgtgggcagc cgacccgtat gatgccgcac gctttgcagg cgtatttttt gccgttatcg
     1201 gactgacttc ttgcggcttt gccggtttca actttttggg cagacaccac gggcgcagcg
     1261 ttgttttaat ccatatcggc tgtatcgggc tgattccggt tgcccatttc ctcaatcccg
     1321 ccgccgccgc ctttgccgcc gccggactgg tgctgcacgg ctactcgctg gcacgccggc
     1381 gcgtgattgc cgcctctttc ctgctcggta cgggttggac gttgatgtcg ctggcggcag
     1441 cttatccggc ggcgtttgcg ctgatgctgc ccctgcccgt gctgatgttt ttccgtccgt
     1501 ggcaaagcag gcgtttgatg ttgacggcag tcgcctcgct tgcctttgcc ctgccgctta
     1561 tgaccgttta cccgctgctc ttggcaaaaa cgcagcccgc gctgtttgcg caatggctca
     1621 actatcacgt tttcggtacg ttcggcggcg tgcggcacat tcagacggca ttcagtttgt
     1681 tttactatct gaaaaatctg ctttggttcg cactgcccgc gctgccgctg gcggtttgga
     1741 cggtttgccg cacacgcctg ttttcgaccg actgggggat tttgggcatt gtctggatgc
     1801 ttgccgtttt ggtgctgctc gcctttaatc cgcagcgttt tcaagacaac ctcgtctggc
     1861 tgctgccgcc gcttgccctg ttcggcgcgg cgcaactgga cagcctgagg cgcggcgcgg
     1921 cggcttttgt caactggttc ggcattatgg cgttcgggct gtttgccgtg ttcctgtgga
     1981 cgggcttttt cgccatgaat tacggctggc ccgccaagct tgccgaacgc gccgcctact
     2041 tcagcccgta ttacgttccc gacatcgatc ccattccgat ggcggttgcc gtactgttca
     2101 cacccttgtg gctgtgggcg attacccgga aaaacatacg cggcaggcag gcggttacca
     2161 actgggcggc aggcgttacc ctgacctggg ctttgctgat gacgctgttc ctgccgtggc
     2221 tggacgcggc gaaaagccac gcgcccgtcg tccggagtat ggaggcatcg ttttccccgg
     2281 aattaaaacg ggagctttca gacggcatcg agtgtatcgg cataggcggc ggcgacctgc
     2341 acacgcggat tgtttggacg cagtacggca cattgccgca ccgcgtcggc gatgtccgtt
     2401 gccgctaccg tatcgtccgc ctgccccaaa acgcggatgc gccgcaaggc tggcagacgg
     2461 tctggcaggg tgcgcgcccg cgcaacaaag acagtaagtt tgcactgata cggaaaatcg
     2521 gggaaaatat attaaaaaca acagattgag ccgaatttct ggattaagtg ccggaaatcg
     2581 cgtataattg cgcgattaaa cccttatata gtggattaaa tttaaatcag gacaaggcga
     2641 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac cggtttaaat
     2701 ttaatccact ataaatcagc cgttttgcag gcatcacaca ggagcgacaa ttattatgat
     2761 gaccctctat tccggcatta cctgcccctt cagccaccgc tgccgcttcg ttttgtacga
     2821 aaaaggtatg gattttgaaa tcaaagacat cgatatttac aacaagcccg aagacctcgc
     2881 cgtcatgaac ccgtacaacc aagtccccgt gctggtcgag cgcgatttgg tgctgcacga
     2941 gtccaacatc atcaacgaat acatcgacga acgcttcccc catccgcagc ttatgcccgg
     3001 cgatcccgtt atgcgcggcc gggggcggct ggtgctgtac cgtatggaaa aagaattgtt
     3061 caaccacgtc caagttttgg aaaaccccgc cgccgccaac aaggaacagg caaaagcgcg
     3121 cgaagccatc ggcaacggcc tgacgatgct ttccccttcg ttcagcaaaa gcaaatacat
     3181 cctcggcgaa gatttttcta tgattgatgt tgccctggcc ccgctgttgt ggcggctcga
     3241 ccattacgat gtcaaactgg gcaaaagtgc cgcgccgctg ctcaaatacg ccgagcgtat
     3301 cttccagcgc gaagccttta tcgaagcact gacacccgcc gaaaaagcca tgcgcaaata
     3361 agtccgaaat gcttgcaaaa cccgccgttt tgcaggcatt ttcacattca ggcacaccac
     3421 acggaaccca tcatgcccac ctcaaccaaa ccctacatcc tccgcgcact ttgcgaatgg
     3481 tgcagcgaca acagcctcac gccgcacatc cttgtctggg taaacgagca tacgcgcgtc
     3541 cccatgcagt acgtccgcga caacgaaatt atgctcaaca tcggcgcaac cgccacgcaa
     3601 aaccttcgaa tcgacaacga ttggatcagc ttttccgccc gtttcggcgg acaagcgcac
     3661 gacatatgga tacctgtcgg acacgtcctc agccttttcg cacgggagac cggagaaggt
     3721 atggggtttg aattggagga gtaccgcccc gacacgccgt ctgaaaacac ttctgccgaa
     3781 accgcgcccc ggcccgccaa aaaaggtttg aaattggtca aataaatcta tgccgtctga
     3841 acggaatcgt gtttcagacg gcattttgtc cgatggggcg caaacggaat ccgtttatcg
     3901 gcaaaacctg tttcggcgta tcgaaaccgt gttgcccctg ccgattcgat attgggacat
     3961 tgaaaatgcc gtctgaacct gcgatacggg cttcagacgg cgttttgtcc gatattcggg
     4021 caatcaggcg gtcagtacgg ttttcaagat tttgttgact tcgcccatat cggctttgcc
     4081 ggcgaggcgg gttttcaata cgaccatcac tttgcccata tccgccatac ctgccgcgcc
     4141 ggtttcggca acggctgctt cgacggcggt gcggatttcg ccggcggaga gcatttgcgg
     4201 caggtagcgg tgcagcacgt cgatttcggc gttttctttg tctgccaaat cctgacggcc
     4261 ggcttcagtg tagattttcg cgccgtcttt gcgctgtttg accattttgg tcaggatggc
     4321 ggtgattttg gcatcgtcgg cttcggtgcg ttcgtctact tcaaactgtt tgacggcggc
     4381 attgatgagg cggatggtgc cgagggaaac ttgatctttg gcgcgcatcg cggttttcat
     4441 atcttcggta aggcgggttt tcaggctcat gatgtcctcg ctgggatgtc ggatggaaac
     4501 ggcgggtttc gatgccgtct gaaaagcaaa acacaccgca gagcaggtct tgcggtgtgt
     4561 cttcatatca cagggctgac ctgtaatctg tacttgaacg tttagtacat tttgggcggc
     4621 agctgttggc tgcgcaggcg tttttgcagg cgttttacgg ctgccgcttt tttgcgtttg
     4681 cgttcggtag tcggtttttc gtaggcttcg cgggcgcgca gctcggtcag caggccggtt
     4741 ttttctacgg cgcgtttgaa acggcgcata gcgacttcaa agggttcgtt ctcttttacg
     4801 cggattgcag gcattttatt tcctttaata aattcggttg ttccatctgc ccatcataat
     4861 cggtggaaag ggtagggcag acggtgttgt aggttttccg tatcgctgtg cctttcggcg
     4921 gcgggatacg cgccgtgacg gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa
     4981 gctgcttgaa aaagtaggaa aaacaatttt cggattgtct tatatttatg gttcgccgtc
     5041 aatgccgttc gggataaaaa tgccgtctga aagaccgggc gggtttcaga cggcatcggc
     5101 acgtcagcgt gcgggaacgg tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa
     5161 gagggaggcg tagtaggcgg cattggtcat cacttttttg acatagtcgc gcgtttcgga
     5221 aaacgggatg gtttcggcat ataccgcgcc ttcgaggggt gtgtccgcct gccatcggcg
     5281 cgccctgccg gggccggcgt tatagcctgc ggtggcgagg atttcgttgt tttgcaggcg
     5341 gcgtttggtg tccgccatat accatgtccc catacggata ttgccgtcgg cggtgtagag
     5401 ctgggcggca tccataccga ttttgccggc gatttcgcgc gcggtggcag gcataacctg
     5461 catcagcccc tgcgcgccta cgcgggattg cgcgcctata acgaagcggc tttcctgacg
     5521 aatcaggccg taaacccaag ccggatcgac attaacattt tgcgcgtggc ggattaccgt
     5581 gtctttaaac ggcgaaatat agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc
     5641 gctgttgacc gccatatcgt aaaaaccgtg gtcgaacgcg gtttgcgcgg cggccagcag
     5701 cttgtcttcg tcaaagccgc gtgtggcaaa acgccattcc gcctgagcct gacggcgcat
     5761 tttcgcatcg ccggcggttc ggctgtgttg gaacaacacc agtgcgcgct taatcgcgcc
     5821 gtcttccgcc atacggagga cgctgttttt gccggcatcg ggaacattgt tgcgcgtatc
     5881 gattttgcgc cccaactctt cccctgccag tacggcataa aaattcctgc ccgtcgctgc
     5941 cgcctgtttg taaagttttt ccgcttcttg cgtgttgccc gttgcggcgc ggctgcgtgc
     6001 cagccagtag agccaggtcg ggcttttttg cagtttttcg ggcatatgcg agatcacgga
     6061 ggccagctcg tcccaacgtc gggcgcgcaa ggtggcgcgg gcgtaccact cgatttggtc
     6121 gtcggtcagt tggcggcggt cggcaacctt gccgtaatag tccaaggcgg caggcacatt
     6181 gaggctttgc gactgataat gccccaatac gccccacgcg aaactgcgtt gttcggggct
     6241 taaaccgctt tccatttcgg acagcagggc ggcggcattc ggcgatttgc gtgcttcttt
     6301 gccgatgacg ttcaacaggg catattcgcg cgaaccttgt gtaccgccgt caaacgggct
     6361 gcccaatgcg gcggcaaggt tgcgcgcgtc cgtcgtttgg cggccggcca gcagtccgcg
     6421 cacgcgcctc caggcatcgt tgccgtccaa caagccggat gcggctgcct gttccaacag
     6481 tttggtgcag cccgaaggca gtttgcccgt atttttgacc agttcggcgg cacgcgtata
     6541 gtcgttgcgg ctcgaatcgg cgtagcattc gacttcttgg gcgcgccctt caggtttgag
     6601 tttggcatat tcctgtgcaa acagcgtcca ctgtctgcgc gcgcccaaag acttcagcca
     6661 ctcgttgcgg acattttccg ccatcgcgct gtcgccggcg ttttccaaat aggcggcggc
     6721 tgccgcatcg ttgttctgtt tcactgcatc cagtgcggac gggtagccgc cgtaatctgc
     6781 cagcgttttt ccttccggtt cggcagggcg ggtgggaacg cttgccgaaa gatcggcagt
     6841 ttctatattg tctgccgggg tcttgccggc tggcagtgtg tttgtcgaag aacacgcggc
     6901 aagcaccagg gccgccagca gcggcaggga atgcttcata gagggtaggt acatcggatt
     6961 tccttaagaa tcggaaccct gaacggtcag ggttggaaaa gacaaaatgc cgtctgaaca
     7021 ggcgtttgcc cgaattatat gccgaaactg caccgtcttt ggaatgtttc cgacataatt
     7081 tatatttttc aatcatttgc cgtttgggtg cgaaccgctg cctttgcccg tttcagacgg
     7141 cattgtccga aatggttgcc cgcttcctgc tttattgaca aaaaaacgct ttcccgataa
     7201 tatcctacga aaattaacct gccgatttga cacagcttgc gggcataaca gctaaagcgt
     7261 tccgacaatt tcagctttat cttccgcgcc cgttgtgtcc gacatcgggc tttgttgtat
     7321 gggaaagaca atgattattt tggacaaggt ttccaagcat taccaaacgc gcgacaaaac
     7381 ccgttttgcc gccgtcgagc cgaccagcct cgaaattcaa gacggcgaaa tcttcgggct
     7441 gatgggctat tccggtgcgg gcaaatccac tttgttgcgc ctgatcaacc tgttggaacg
     7501 ccccgacacg ggcaaagtca acgtctgcgg acaagaactg accgcgctcg atgccgccgc
     7561 attgcgtcag gcgcggcaga atatcggcat ggtgtttcag cagttcaacc ttttaagcaa
     7621 ccgcaccgtc gccggcaacg ttgcctttcc actggaaatc gccggatggc cgtctgaaaa
     7681 aatcaaagcg cgcgttgccg agtgcctcga aatcgtcggc ttgaccgaac gcgccggcca
     7741 ctaccccgcc cagctttcag gcgggcagaa acaacgcgtc ggaatcgccc gcgcgctcgc
     7801 gcccaagccg caagtcatcc tcgccgacga acccacttcc gccctcgacc ccgccaccac
     7861 gcgcagtgtc ttggaatgtt tggaagacat caacaaacgc ttcaacgtta ccatcgtcat
     7921 cgtaacccac gaaatgagcg tcatcagacg attgtgcgac cgcgccgccc tgttggataa
     7981 agggaaagtg gtggaaatcg tcgaagtacg cggcaaccaa atccacgctc aatccgaaat
     8041 cgggcgcgaa ctgattcggg aggactgata tggcaggctt aacattcgaa caagccgttt
     8101 ccaccatcgt cggcatgaaa gacgaaatcg tccgcgcctt gggcgaaact ttcgtgatgg
     8161 tcggcttgtc caccacattc gccgtcatct tcggcacgct cttgggcgtg ttgctcttcg
     8221 taacttccag ccgccaactg cattacaaca agccggtaaa cttcctgctc gacaacctcg
     8281 tcaacctcat gcgcgccttc cccttcgtca tcctgatgat tgcgatgata cccgccacac
     8341 gcgccatcgt cggcagcacc atcggcccgg ttgccgcctc gctggtgttg agcgtgtcgg
     8401 gattgtttta ttttgcccga ctggtggaac aaaacctgcg cgaagtccca aaaggtgtga
     8461 ttgaagccgc cgccgcgatg ggtgcgccgc cgattgccat cgtcctcaaa gtcctcttga
     8521 acgaagcgcg cgcgggcatg gtttccagca ttaccgtgct tgccatcggg cttttgtcat
     8581 acagcgcggc ggcaggcatg ataggcggcg gcggcttggg cgacctcgcc atccgctacg
     8641 gctactaccg ctaccaaacc gaagtcatcg tcttcatcgt cgccctcctc gtgctgctgg
     8701 tcatcctgat tcaaagcacc ggcaacgcgt tggcgcggaa actcgacaaa cgttaaaccc
     8761 gaatgccgtc tgaacgccaa aaaccccgcc gctatccgaa aaatgctata aaatccccct
     8821 gttcgcggca aatgccgtct gaacgccgaa tccggacggc aggactccct gcctgtcatt
     8881 tttgtttgaa accgccacaa catcaggaga aaatatgaaa accttcttca aaaccctttc
     8941 cgccgccgca ctcgcgctca tcctcgcagc ctgcggcggt caaaaagaca gcgcgcccgc
     9001 agcctctgcc gccgcccctt ctgccgataa cggcgcggcg aaaaaagaaa tcgtcttcgg
     9061 cacgaccgtc ggcgacttcg gcgatatggt caaagaacaa atccaagccg agctggagaa
     9121 aaaaggctac accgtcaaat tggtcgaatt taccgactat gtgcgcccga atctggcatt
     9181 ggcggagggc gagttggaca tcaacgtctt ccaacacaaa ccctatcttg acgatttcaa
     9241 aaaagaacac aacctggaca tcaccgaagc cttccaagtg ccgaccgcgc ctttgggact
     9301 gtatccgggc aaactgaaat cgctggaaga agtcaaagac ggcagcaccg tatccgcgcc
     9361 caacgacccg tccaacttcg cacgcgcctt ggtgatgctg aacgaactgg gttggatcaa
     9421 actcaaagac ggcatcaatc cgctgaccgc atccaaagcc gatatcgcgg aaaacctgaa
     9481 aaacatcaaa atcgtcgagc ttgaagccgc acaactgccg cgcagccgcg ccgacgtgga
     9541 ttttgccgtc gtcaacggca actacgccat aagcagcggc atgaagctga ccgaagccct
     9601 gttccaagag ccgagctttg cctatgtcaa ctggtctgcc gtcaaaaccg ccgacaaaga
     9661 cagccaatgg cttaaagacg taaccgaggc ctataactcc gacgcgttca aagcctacgc
     9721 gcacaaacgc ttcgagggct acaaataccc tgccgcatgg aatgaaggcg cagccaaata
     9781 aggcagtcgt ataaaatgat gccgtctgaa ccgcattccg tgttcagacg gcatttttgt
     9841 cctttaatcc gccattccct gccattccgc caaatccggc gtatcgattc cgaacagcga
     9901 cagcgtgtgt gcgatactgt gcgccactat gtcgtccgcc gtctgcggct tgcggtacat
     9961 cgcaggaaca gggggaaaca ccacgccgcc catttccgtt acccgcttca tattgtccaa
    10021 atgggcaagg ttcagcggcg tttcgcgcac catcagcacc agccgccgcc tttccttcaa
    10081 aaccacatcc gccgcacgcg tcaagagatt gtcgccgaag ccgtgcgcga cagaggcaag
    10141 cgtccgcatc gaacagggtg cgaccagcat cccgtccgtt ttaaacgtac cgctggcaat
    10201 gcacgccccg atattgccga tcggatggac gaaatcagcc aaggcatata cttcgtcttt
    10261 cgtataatcc gtttccgaag cgcgcgccat ctccgcgcct ttcgatacca caaggtgcgt
    10321 ttcgacatct tgcgcgcgca aaagttccaa agccttcacg ccgtattgga aaccgctcgc
    10381 cccgctgatg ccgattatca aacgccgtac catcatccgc ctttcccata aaaccgcctg
    10441 caacggcaaa ccggctatta tagtgaaaaa acagaaatcc gataaacacg gatacaaatt
    10501 gtcggcaaca cccaatatcc gataaaatac ccgatttaac atcctatctg aataggcacg
    10561 ggagggcggt atggcaaaag taaaaggcgg attggggcgc ggcttggatt cgctgctcgc
    10621 caacggtgcg gacaacagca gcggcgaccg gttgactgcg gttgcggtta aagatatccg
    10681 gcccggccgc tatcaggcgc gcgtccaaat cgatgacgaa gccttgcagg aactggcaga
    10741 ttcgattaag gcgcaaggcg tgatacagcc cgtcatcgtg cgcgaacacg gactgtcccg
    10801 atacgaactg attgcaggcg aacgccgttg gcgcgccgct cagattgccg gcctgtccga
    10861 aatccccgcc gttatcaaaa ccatcagcga cgaaaccgca ttggcaatgg gtttgatcga
    10921 aaacctccag cgcgaaaacc tcaaccccat cgaagaagca caaggcttga aacgccttgc
    10981 cgacgagttc gggctgaccc acgaaaccat cgcccaagcc gtcggcaaaa gccgaagcgc
    11041 gatttccaac agcctgcgcc ttttaagcct gcccgaatcc gtgcaggaaa tgctttacca
    11101 acgccgcctc gaaatggggc acgcccgcgc actgctgacc ctgcccgtcg tcgaacagct
    11161 cgaattggcg caaaaggccg tcaaaaacgg ctggtcggtg cgcgaagtcg aacgccgcag
    11221 ccaggccgcc cttcaaaaca aacgtcccga gccaaaaaaa actgccgccg ccgacatcgg
    11281 ccgcctgaat gatttgctga ctgaaaaatt gggtgtcaac gccgaaatca agaccgccaa
    11341 ccataaaaaa ggcaaaatca tcctgcattt cgacacgccc gaaaccttcg accatatctt
    11401 gaagcagttg ggcatagatt accggcctta attttgcggg atatgccgtt tgaaatatag
    11461 agaatagctt tccagatttt aagtgggaaa tataattcta ttgacatttt ttctgcttcg
    11521 cgtaagaatc gttttcctgt tttcattttt aattttcgag gaaattatga acacacgcat
    11581 caactttacg caactgtggg taagcgcgtg agccaatgct atgttaccag tgtaatcagc
    11641 acaatcggcg ttaccacttc cgatgcaatt tgttcgggag gcggaacgaa caaagccaaa
    11701 agtcaatgca atgctttgct taaagcggca ggccgctgct aatcctttat tcggaaaagg
    11761 tcgtctgaaa atattttcag acgacctttt atttattgag caaactcccc aaactgccga
    11821 caatccgacc gataaactac tacaatttcg cgttgatttt ttattatttc cacgtaaagc
    11881 aaacaaatca cgtaagaaca ttaaaagtgt aaagaaattg cggacaaact ccttaatatc
    11941 ctgtaatcat atgtaatatg tgttagaata gcggagttgg attttgggga gtcgctcgtc
    12001 tgtatcggcg gcgagtttca caaagattaa catattgctt gacgtaataa ggcttctaaa
    12061 ttatattagc cgcgccccaa acctgaggcg gaaacattcc ctttatgaag cagattatca
    12121 tcctacaatc cgccgtattg tccatatgcg ccgcagttgc ctttgccgta tggggttttg
    12181 ccggattcct ttcggcggtc gggggcggtt tgtcctacct gcttcccacg tttgttgcag
    12241 ttttacttct aaaacttttc aggggaaacc ccttcctgca aagcaggatg ttcgtcttcg
    12301 gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct tgccgtattt gcaatatggc
    12361 atcaatcgct ggtgttcgtc ccgtttttga tggggctgct cggtgtcagc catctggttt
    12421 ttttagtatt gttgagagtg aaagattatg gcaggtgaaa ccattaccgc tgccgactac
    12481 atcaagcacc acttgcagag cttgaccagt ttgtcggatg ttactcaggg tcagggactg
    12541 aaaaacattg ctgatttttc gtttattaac cttgatgccg ttttttttgc cgtcctgttg
    12601 ggcgtaatcg gcagcttcct gttgtggcgc ggtgcaaaaa aagcaacggc aggcgttccc
    12661 ggacgtttcc aggccgccgt ggaaatcttg ttcgagtttg tggacgatat gtgtaagagc
    12721 atcattcaca gcgaaaagtc tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtt
    12781 tggattttcc tgatgaacgc gatggatatg ctgccggtcg atttgctgcc gatggtatgg
    12841 cagggtatta ccggcaacca tcacgccctg ctgcgcatcg taccgactgc cgatttgaac
    12901 accgctttgg cactcgctgt cggcgtgttg ctgatttgta tctattacaa catcaaaatc
    12961 aaaggattgg gcggctggtt tcacgagttg ttcagtgcgc cgttcggtgc aaaactcgca
    13021 cctgcgaact ttctgttgaa cttggtcgag tttctttcta aaaccgtatc ccacggtatg
    13081 cggttgttcg gcaatatgta tgcgggcgag ctggtattcc tgctgatagc cttgcttggc
    13141 ggtgcttggg cggcttccgg cagcgttgaa gtcatggatc cgattctgtt tgtattccac
    13201 attattgccg gtttggcatg ggcgattttc catattttgg tgattaccct gcaggcattt
    13261 attttcatgg cgttggcgtt cgtctatatc gggcaggcgc acgatgccca ctgattttcc
    13321 caagtagttt gtttttgtag tttaaccttt gtttctttaa ggagtttaaa atgggtttga
    13381 ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt gggtgcatct atcggtatcg
    13441 caatggtcgg ttctaaatat ttggagtctt ctgctcgcca acctgaactg attggtccgc
    13501 tgcaaaccaa actgttcctg attgccggtc tgattgatgc cgcattcttg atcggtgtcg
    13561 ccattgcact actgttcgcc ttcgtcaacc cgtttgcagg tgcataatca ggacggattc
    13621 gtccgttcgt acaggtgcag cctgtttgat taaccccaat acgaaggtta agtaacgtga
    13681 atatcaatgc aacattattc gctcaaatca tcgtcttttt cggtttggta tggtttacca
    13741 tgaaatttgt gtggccgccg attgcaaaag ctttggatga gcgtgccgca aaaatcgccg
    13801 agggcttggc tgccgccgag cgtggtaaaa gcgatttcga gcaggctgaa aaaaaggttg
    13861 cagaactttt ggcagaaggg cgtaatcagg tttccgaaat ggttgccaac gccgaaaaac
    13921 gtgccgccaa aattgtcgaa gaagccaaag aacaggcttc ttccgaggcg gcgcgcattg
    13981 cagctcaggc aaaggccgat gtggagcagg aattgttccg cgcacgcgaa tccctgcgcg
    14041 atcaggttgc cgtgttggct gtcaaaggtg ccgaatctat tttgcgcagc gaagtcgatg
    14101 cttccaaaca cgcaaaactg ctcgataccc tgaaacagga gttgtaatct tatggcagag
    14161 ttcgcaacga tcgccagacc ttatgcaaag gcattgttcg gtctggctca ggaaaaaaac
    14221 caaattgagt cttggttggg cggactggaa aaacttgcgg cggttgttca ggaagggaag
    14281 gtggcttcat tgattgaccg tcctgaaacg aatgcttcag aaaaagcaga tatcctcatc
    14341 gatttggtcg gtttgaaaga caaggagtta aaaaactttg ttatcgtctt ggccgggcag
    14401 aaacgtttgt cgatattgcc ggaagtgtat gctcaatatc aagacttgac cttatcattc
    14461 aaccatatca aatctgccgt catttacagt gcctatccgt tgaccgacaa acaggtcggc
    14521 gagttggcgc aaatgctgaa taagcgtttc gacagcgagc tgaaaatctc tgtcgaaatc
    14581 gaaccggagc tgattggcgg cataaaagtt gaagtgggtg atcaggtttt ggatttgtct
    14641 gtacaaggca aactgagtgc tttgtacacg actatgacga attaggagag ttttcatgca
    14701 gcttaatcct gctgaaatta gcgatctgat taaagccaag atcgaaaatc tgtctgtaaa
    14761 tgccgaagtg agtacccgtg gtacggtgat ttcggtaact gacggtatcg ttcgcatcca
    14821 tggtttgtca gatgcaatgc aaggtgagat gctcgaattc ccgggtaaca ctttaggctt
    14881 ggcgatgaac ctggagcgcg actccgtcgg tgccgtagtg ttgggcgagt acgaacatat
    14941 taaagaaggc gacacggtta cctgtaccgg ccgtatttta gaagtgccgg tcggacgcga
    15001 attggtcgga cgcgtcgtcg atgcattggg tcggcctatc gacggcaaag gcccgattaa
    15061 tacaactttg accgcccctg tcgaaaaaat cgcaccgggc gtgattgcac gcaaatcggt
    15121 tgatcagccg atgcaaaccg gtctgaaggc gattgactct atgattcctg tcggtcgcgg
    15181 tcagcgtgag ttgattattg gagaccgtca gacaggtaaa acggccgtgg cattggatgc
    15241 catcgtcaac caaaaaggta cgggtgttat ctgtatctat gtcgctatcg gtcaaaaagc
    15301 atcttctatt gccaacgtgg tccgcaaatt ggaagagcat ggcgcgatgg aacacacgat
    15361 tgtggttgct gcaactgcat ctgaagcggc ggcattgcaa tatatcgcac cttattcagg
    15421 ttgtacgatg ggtgaattct tccgcgatcg tggcgaagat gccttgattg tttatgacga
    15481 tttgtccaaa caggctgtgg cttaccgtca aatttccctg cttttgcgcc gtccgcccgg
    15541 ccgcgaagcc tatcccggcg atgtgttcta cctgcactcc cgtctgttgg aacgtgcggc
    15601 acgtgtcaat gaacacgaag tggaaaaatt gaccaacggc gaagtaaaag gcaaaaccgg
    15661 ttctctgacc gcgttgccga ttatcgaaac ccaagcgggc gacgtatctg ctttcgttcc
    15721 gactaacgtc atttcgatta ccgacggtca gattttcttg gaaaccgacc tcttcaacgc
    15781 cggtatccgt cctgcaatca atgccggtat ttccgtatcc cgcgtaggcg gtgctgcaca
    15841 aaccaaagtg attaaaaagc tgggtggcgg tatccgtttg gcgttggcac aatatcgtga
    15901 attggcggcg ttctcgcaat ttgcatccga tttggatgaa gctacgcgca aacagttgga
    15961 gcatggcgaa gtcgtaaccg aactgatgaa acagaaacag ttcagtacgt tgaatacggc
    16021 tgaaatggct ttgacccttt gggcaatcaa caacggttcg tattctgatg ttccggttgc
    16081 caaagctcta gcttttgaat ctgaattttt gagctttgtc cgtacccaac atccggaagt
    16141 tttggaagcc gtcaatgctt caggtgcaat gtccgacgag agcgagaaaa cgcttgaagc
    16201 agccatgaaa tccttcaaat cttcttacgc ctatcaggca taaggcttga agtgaaagga
    16261 gtctgaaatg gcagtaggaa aagagattct caccaaaatc cgcagtgttc agaataccca
    16321 aaagatcact aaagcgatgc aaatggtgtc aacctctaaa atgcggaaga ctcaggaacg
    16381 gatgcgtttg gcgcgtccgt atgccgaaaa agtgcgtatg gtgatgagcc atcttgcgca
    16441 aaccaatacc gatcatggta ttccgttact ggaatctcat cgggaaatca gacgtgtcgg
    16501 ttttattttg attacgtctg ataagggttt gtgtggcggt ttgaacgcca acgtgctgaa
    16561 aaagtttttg gcacaagttc aagagtatcg gaatcaaggt attgaagagg aaatcgtatg
    16621 ccttggcagt aaaggtctga tggcgtgtca gagcattggt ctgaatgtgg ttgccagtgc
    16681 cgtaaatttg ggcgataccc caaaaatgga aatgctgctc ggacctttga cagaactttt
    16741 ccaacggtat gagaaacatg aaattgacaa aatccatctg gtgtattcgg gttttgtcaa
    16801 taccatgcgt caagaaccga ggatggaagt attgctgcct atcggtgaga acgtgattgg
    16861 cgattcagct cccaaaccac cgttcagctg ggaataccgc tacgaaccga ctgcacttgc
    16921 agtgttggaa tatctggttc gccgctattt agagtctgtg gtttatcagg cgttgagcga
    16981 caatatggca tccgaacagg cagcccgcat ggttgccatg aaagctgcaa cagacaatgc
    17041 aggcaatgcc atcaaagagt tgcgtctggt atataacaaa tcgcgtcaag ctgcgattac
    17101 cacggaattg tcagaaattg tagcaggtgc ggcggccgtc tgatgccgtc tgaatcctgt
    17161 acggaaatta ggatacgata atgagccaag gcaaaatcgt acaaattatc ggtgcggttg
    17221 ttgacgtgga atttccacgc gacatgattc cgcgcgttta cgacgctttg aaattagacg
    17281 aaaacggtct gactttggaa gtccaacagc ttttgggtga tggcgtagtc cgtaccatcg
    17341 ctatgggcag ctcggacggt ttgaaacgcg gcatgactgt gagcaatact ggttcgccca
    17401 ttactgtgcc ggtaggtaaa ggtacgttgg gacgcattgt cgatgtattg ggaactcctg
    17461 ttgacgaggc aggtccaatt gataccgaca agagtcgtgc catccaccaa gccgctccta
    17521 agtttgacga actgtcttcc acaaccgaat tgctcgaaac gggcattaaa gtgattgact
    17581 tgctgtgtcc gtttgccaaa ggcggtaaag taggtctgtt cggcggtgcc ggtgtgggta
    17641 aaaccgtgaa catgatggaa ttgatcaaca acatcgccaa agcgcacagc ggcttgtccg
    17701 tgttctcagg cgtgggtgag cgtacccgcg aaggtaacga cttctaccac gagatgaaag
    17761 attccaacgt attggataaa gtagccatgg tgtatggcca aatgaacgaa cctccaggca
    17821 accgtctgcg cgttgctttg accggtttga ctatggctga atacttccgt gacgaaaaag
    17881 acgaaaacgg taaaggtcgt gacgtattgt tcttcgttga caacatctac cgttacactc
    17941 tggccggtac cgaagtatcc gcactgttgg gccgtatgcc ttctgcagtg ggttaccaac
    18001 cgacattggc tgaagaaatg ggtcgtttgc aagagcgtat tacctctacc caaaccggtt
    18061 ccattacttc catccaagcc gtatatgtac ctgcggatga cttgactgac ccgtctccgg
    18121 cgacaacttt cgcccacttg gacgcgaccg tcgtattgag ccgtgatatt gcctctttgg
    18181 gtatttaccc ggcagttgac ccgcttgact ctacttcgcg ccaattggat ccgatggtat
    18241 tgggtcaaga gcactacgac gtagcgcgcg gtgtacagtc tactctgcaa aaatacaaag
    18301 aattgcgcga catcatcgcc attctgggta tggacgaatt gtctgacgaa gacaaactgg
    18361 ctgtaatgcg tgcccgtaaa atccaacgct tcctgtctca accgttccac gttgccgaag
    18421 tgtttacagg ttctccaggc aaatatgtcg ccctgcgcga taccattgcc ggcttcaaag
    18481 ccatcttgaa cggcgaatac gatcatctgc ccgaacaggc attctatatg gtcggcagca
    18541 tcgaagaagc ggttgagaaa gcgaaaacct taaactgagg aggtcggcat gagcatcatg
    18601 caagttgagg tggtaagtgg cgagcagaaa atctattcag gcgaggcaac atttatcgtt
    18661 gttccgactg tacagggcga actcggtatt tatccgcgac acgagccgat tatgagtttg
    18721 gtgcggccgg gggctttgcg tttgaccgtt ccgggcgagg ataaagaggt tttggttgct
    18781 gtttccggcg gtattttgga agtacagcct gataaagtaa ctgtcttggc ggatgttgcc
    18841 gtccgcagtg cggaaatgga tcgggcacgt gcggaagagg cgaaaaaagc cgcagaagcg
    18901 ggcatttccc aagctaaaga cgataaggct ttggcggaag cacataaagc attggctgct
    18961 gcgattgcgc agctcaaaac tttggactat atccgttcgc acaagaaata aacttgtttg
    19021 gtttgaaaaa gcacgaccgt ttggccgtgc tttttatttt ccggatttaa attaaaaaat
    19081 tatccatccc ctggtttcaa atcaggctgt cttcgagata aatctattca agcggctatg
    19141 gaaacagtaa aataaaacct ttattttatc gaacaataag ccatgcttac cttccaacaa
    19201 atcatcttca aactgcaaac cttctgggca gacaaaggct gcaccgtcat ccaacccttc
    19261 gacatggaag tcggtgccgg cacatcccat cccgccacct gcctgcgcgc gctcggcccc
    19321 gagccttggt ttgccgccta cgtccaaccc agccgccgcc ccaaagacgg ccgctacggc
    19381 gacaacccca accgcctgca acactattac caattccaag tcgccctcaa acccgccccc
    19441 gccaatatcc aagacctcta tctcgactcc ctgcgcgaat tgggcatcga tcccaaagtc
    19501 cacgacatcc gctttgtcga agacgactgg gaaaacccca ccctcggtgc gtggggtttg
    19561 ggctgggaag tctggctcaa cggcatggaa gtaacccagt ttacctattt ccaacaagtc
    19621 ggcggtatcg attgcacgcc cgtactcggc gaaattacct acggcatcga acgcttggcg
    19681 atgtacctgc aaggcgtgga aaacgtctat gacctcgttt gggcaaaaac gccggacggc
    19741 aataccgtaa gctacggcga cgtgtaccat caaaacgaag tcgagcaatc tacctacaac
    19801 ttcgaataca gcgatgccga ctggctgctg cgccagttca acgactacga agcgcaagcc
    19861 aaacgcctgt tcgccgaaga aaacgccgac cttgccttac ctgcctacga gctggtcctc
    19921 aaagcggggc atacgttcaa ccttttagac gcacgcggcg cgatttccgt aaccgagcgg
    19981 gcaacttata ttggacgtat tcgtgcattg agccgcgccg tggcgcaaaa atatatcgaa
    20041 agccgtgaga aactgggctt cccgttgatt aaaaaataac gatacgtcgt ctgaagcagg
    20101 ttttcaggcg acctttattg cgtatccatt aatacaggcc gtacggcata tgcaggcttt
    20161 ataagcatgg cctacggaca aacagaaagt ctcacatgac aacccaaacc cttttaatcg
    20221 aactccttac cgaagaactc ccgccaaaag ccctgaataa tctgggcaac cattttgccg
    20281 cttccgttgc cgaaggcttg gaaaaagcgc aactggttga cggcgcggcc gaatttacgg
    20341 cttatgcctc gccgcgccgt ttggcggttc aagtcaaaaa cgtcaaagct gttcaggccg
    20401 atcagaaaat cgtgaaaaaa ggccctgccg tggcgaatgc cgtaaaagac ggtacgccga
    20461 ccaaggcttt ggaaggtttt gcgcgcgggg cgggggcgaa aatcgaagac ctgaccatca
    20521 tccacgacgg caggcaggac gtgtacgcct acgaatacgt ccaaaccggc agaccgttgg
    20581 gcggactttt ggaagacatt atcaatcaag ctgttaagaa gctgccgatt ccgaaagtaa
    20641 tgcgttgggg cagcagcacg tttaccttcg tgcgccctgt tcacgggctg attgtgctgc
    20701 acggcggcga tgtcgtgaac gtcagcgttt tgggcctgca aagcggcaat caaaccttgg
    20761 gacaccgctt tctttccgac ggcgaaatga tcattgaaaa cgccgacagc tacgccgcac
    20821 aaatgcgcgg gcaaggcaaa gtcgtcgctt cgtttgccgg gcgcaaagcc gcgattcaga
    20881 cggcattgga agggcaggca cgccgtctga acgcgaccgt tgccgccgat gaagccctgt
    20941 tggacgaagt gaccgcgctg gtcgaatggc ctgttgtttt ggaagccggt tttgaggaac
    21001 acttcctcgc cgtgcctcaa gaatgcttga ttttgacgat gcagcaaaac caaaaatact
    21061 tcccgctgct cgaccaaaac ggcaagctga tgaaccgctt cctgctggtt tccaacctgc
    21121 aaaccgaaga cccgtcgcac atcatccgag gcaacgaacg cgtattgcgc gcgcgcctgt
    21181 ctgatgccga gttcttctac aaacaagacc aaaaagcgac cttggaaagc cgcctgccca
    21241 agctggcgaa cgtggtgtac cacaacaaaa tcggttcgca ggccgaacgc atcgaacgcc
    21301 tgcaaagcat cgccgcccac atcgccaaag cattgggcgc ggatgccgcg gcagccgagc
    21361 gcgccgcgcg tcttgccaaa gccgacttgg taactgaaat ggtcggcgag ttccccgaac
    21421 tgcaaggcac gatgggcaaa tactacgccc gcttggacgg cgaaaccgaa gaaatcgccg
    21481 aagctattga gcaacactac caaccgcgtt ttgccggcga caagctgccc gaaagcaaaa
    21541 ttgccgccgc cgtggcactt gccgacaaac tggaaaccct ggtcggcatt tggggcatcg
    21601 gtttgattcc taccggcgac aaagacccct acgccctgcg ccgcgccgcc ttgggtattt
    21661 tgcggatgct gatgcagtac ggtttggacg tgaacgaact gattcagacg gcattcgaca
    21721 gcttcccgca aggtttgctc aacgaaaaaa cgccgtctga aaccgccgac tttatgcagg
    21781 cgcgccttgc cgtgttgctg caaaacgatt atccgcaaga catcgttgcc gccgtactcg
    21841 ccaaacagcc gcgccgtttg gacgatttga ccgccaaact gcaggccgtc gccgtattca
    21901 aacaactgcc cgaagccgcc gcgctcgccg ccgccaacaa acgcgtgcaa aacctgctga
    21961 aaaaagccga tgccgcgttg ggcgcagtta acgaaagctt gctgcaacag gacgaagaaa
    22021 aagccctgta cgctgccgcg caaggtttgc agccgaaaat cgccgccgcc gtcgccgaag
    22081 gcaatttccg aaccgccttg tccgaactgg cttccgtcaa gccgcaggtt gatgccttct
    22141 tcgacggcgt gatggtgatg gcggaagatg ccgccgtaaa acaaaaccgc ctgaacctgc
    22201 tgaaccgctt ggcagggcag atgaacgcgg tggccgacat cgcgcttttg ggcgagtaac
    22261 cgttgtacag tccaaatgcc gtctgaagcc ttcaggcggc atcaaattat cgggagagta
    22321 aattgcagcc tttagtcagc gtattgattt gcgcctacaa cgtagaaaaa tattttgccc
    22381 aatcattagc cgccgtcgtg aatcagactt ggcgcaactt ggatattttg attgtcgatg
    22441 acggctcgac agacggcaca cttgccattg ccaaggattt tcaaaagcgg gacagccgta
    22501 tcaaaatcct tgcacaagct caaaattccg gcctgattcc ctctttaaac atcgggctgg
    22561 acgaattggc aaagtcgggg ggggaatata ttgcgcgcac cgatgccgac gatattgcct
    22621 cccccggctg gattgagaaa atcgtgggcg agatggaaaa agaccgcagc atcattgcga
    22681 tgggcgcgtg gctggaagtt ttgtcggaag aaaaggacgg caaccggctg gcgcggcacc
    22741 acaaacacgg cgaaatttgg aaaaagccga cccggcatga agacatcgcc gccgttttcc
    22801 ctttcggcaa ccccatacac aacaacacga tgattatgcg gcgcagcgtc attgacggcg
    22861 gtttgcgtta cgacaccggg cgggattggg cggaagatta ccaattttgg tacgatgtca
    22921 gcaaattggg caggctggct tattatcccg aagccttggt caaataccgc cttcacgcca
    22981 atcaggtttc atccaaacac agcgtccgcc aacacgaaat cgcgcaaggc atccaaaaaa
    23041 ccgccagaaa cgattttttg cagtctatgg gttttaaaac ccggttcgac agcctagaat
    23101 accgccaaac aaaagcagcg gcgtacgaat tgccggagaa ggatttgccg gaagaagatt
    23161 ttgaacgcgc ccgccggttt ttgtaccgat gcttcaaacg gacggacacg ccgccctccg
    23221 gcgcgtggct ggatttcgcg gcagacggca ggatgaggcg gctgtttacc ttgaggcaat
    23281 acttcggcat tttgtaccgg ctgattaaaa accgccggca ggcgcggtcg gattcggcag
    23341 ggaaagaaca ggagattt
//
LOCUS       DAXDZG010000027        23251 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXDZG010000027 DAXDZG010000000
VERSION     DAXDZG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23251
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.027"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(142..1530)
                     /locus_tag="ACFB78_000944"
     CDS             complement(142..1530)
                     /locus_tag="ACFB78_000944"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC1268963.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1858..2805
                     /locus_tag="ACFB78_000945"
     CDS             1858..2805
                     /locus_tag="ACFB78_000945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1268964.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB78_000946"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB78_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC1268965.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB78_000947"
     CDS             3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB78_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC1268966.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB78_000948"
     CDS             complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB78_000948"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC1268967.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHRTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB78_000949"
     CDS             5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB78_000949"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC1268968.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            6906..8447
                     /locus_tag="ACFB78_000950"
     CDS             6906..8447
                     /locus_tag="ACFB78_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC1268969.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(8506..9555)
                     /locus_tag="ACFB78_000951"
     CDS             complement(8506..9555)
                     /locus_tag="ACFB78_000951"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC1268970.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(9564..9716)
                     /locus_tag="ACFB78_000952"
     CDS             complement(9564..9716)
                     /locus_tag="ACFB78_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268971.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            9776..10408
                     /gene="pdxH"
                     /locus_tag="ACFB78_000953"
     CDS             9776..10408
                     /gene="pdxH"
                     /locus_tag="ACFB78_000953"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC1268972.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(10538..11548)
                     /locus_tag="ACFB78_000954"
     CDS             complement(10538..11548)
                     /locus_tag="ACFB78_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC1268973.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPIRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(11775..13205)
                     /gene="gatB"
                     /locus_tag="ACFB78_000955"
     CDS             complement(11775..13205)
                     /gene="gatB"
                     /locus_tag="ACFB78_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC1268974.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            complement(13248..14180)
                     /locus_tag="ACFB78_000956"
     CDS             complement(13248..14180)
                     /locus_tag="ACFB78_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC1268975.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            complement(14177..15622)
                     /gene="gatA"
                     /locus_tag="ACFB78_000957"
     CDS             complement(14177..15622)
                     /gene="gatA"
                     /locus_tag="ACFB78_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC1268976.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(15685..15975)
                     /gene="gatC"
                     /locus_tag="ACFB78_000958"
     CDS             complement(15685..15975)
                     /gene="gatC"
                     /locus_tag="ACFB78_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC1268977.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            16125..16778
                     /locus_tag="ACFB78_000959"
     CDS             16125..16778
                     /locus_tag="ACFB78_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC1268978.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            16783..18126
                     /locus_tag="ACFB78_000960"
     CDS             16783..18126
                     /locus_tag="ACFB78_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC1268979.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAVDRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(18175..18603)
                     /locus_tag="ACFB78_000961"
     CDS             complement(18175..18603)
                     /locus_tag="ACFB78_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1268980.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            18751..18891
                     /locus_tag="ACFB78_000962"
     CDS             18751..18891
                     /locus_tag="ACFB78_000962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268981.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            complement(18949..20205)
                     /locus_tag="ACFB78_000963"
     CDS             complement(18949..20205)
                     /locus_tag="ACFB78_000963"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC1268982.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(20241..21110)
                     /locus_tag="ACFB78_000964"
     CDS             complement(20241..21110)
                     /locus_tag="ACFB78_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC1268983.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            complement(21151..21966)
                     /locus_tag="ACFB78_000965"
     CDS             complement(21151..21966)
                     /locus_tag="ACFB78_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC1268984.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            22210..22995
                     /locus_tag="ACFB78_000966"
     CDS             22210..22995
                     /locus_tag="ACFB78_000966"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC1268985.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYSPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         4749 a         5885 c         6826 g         5791 t
ORIGIN      
        1 gtaccggttt aaatttaatc cactatattg cagattaatc ctttgggtat caaagccggg
       61 cggtgctgaa acattacaaa aatgccgtct gaaactcgaa atcggtttca gacggcattg
      121 gtttacgcgg caggttgggg cttagcggtc tttgtaacgg cgttcgcttt tgcctgcaaa
      181 agagtcgccg gctttgcgtt tgcctttgaa gccttcgccg gtttttttga aactgtcttt
      241 cttaaagcct tctttcttga aaccttcgcc gcgcgttttg ccgccgaagc cttctttgcc
      301 cggtttatga tcgccgcgcc ggccgccgga tttcctatcg ccccagccgc ctttgccttt
      361 cggcttgccg cctgcggatt tgcgtttgcg ggccggctcc atgccttcga tggtcagttc
      421 gggcagtttg cggttaatgt atttttcgat tttgtggact ttgacgtatt cgttcacttc
      481 ggcaaacgta atcgcaatac ccgtgcggcc tgcgcggccg gtgcgcccga tgcggtggac
      541 gtagtcttcc gcctgtttcg gcaggtcgta gtttatgacg tgggtaatgg tcggtacgtc
      601 aataccgcgt gcggcaacgt cggtggcaac caaaattttg cagcggcctt tacgcaaatc
      661 cgtcagcgtg cggttgcgcc agccctgcgg catatcgccg tgcaggcagt tggcggcgaa
      721 acctttttcg tacaattcat ccgcgatgac ttcggtcatc gctttggtgg acgtgaaaat
      781 cacacattgg tcgatgttgg catcgcgcag gatgtggtcg agcaggcggt ttttgtggcg
      841 catatcgtcg cagtacaaca actgctcttc gattttgcct tggccgtcca cgcgttcgac
      901 ttcgataatt tcagagtctt tggtcagttt gcgcgccagt ttgccgactg cgccgtccca
      961 agtggcggag aacaataaag tctgacggtc ggccggggtg gcttccacga tggtttcgat
     1021 gtcgtcgata aagcccatat ccaacatacg gtcggcttcg tccaaaatca gcacttccaa
     1081 acgttcaaaa tcaactttgc cgctttgcat caggtccgtc agacggcccg gagtggcgac
     1141 aatcaaatcg accggtttgc tcagggcgcg ggtttggtag ccgaacgatg tgccgccgac
     1201 gatgctgacg gtgcggaacc agcgcatatt tttggcatac gccagcgcgt ttttctcgac
     1261 ttgcgccgcc agttcgcggg tcggtgccaa caccagcgcg cgcgggcctt tgcccggttt
     1321 ttcgctgcgt ttggtcagtc gctgcaaagt cggtaacaga aaggctgcgg tttttccgga
     1381 gccggtttgc gccgaagcca tgatgtcgca gccttccaaa gcaaacggaa tagcttgcgc
     1441 ctgaatcggc gtcgggcttt cgtaaccctc gctgctgacg gcggacaaaa tgtttttatc
     1501 aaggttcaaa tcggcaaatt taatagacat ggctatcctt ggaagacaac aacgcccgcg
     1561 tccgaaaggt tcagacggcc tgaagcaggg aagggtaacg atacggggaa tgtgaagtta
     1621 cagggttgtc gcaagcggct tgcttgtggt ttacatcgac ggcaacctgc acagatacgc
     1681 tttgcaagat caaagcaaca aaagaaacgc gcccgtaagg gcggtttagg ttggaaggcg
     1741 atggcttcgt atgacgatat aagcagagaa tggtataggg tttgcccgaa aaggtcaagc
     1801 aatcgggcgt taattacagt aaaatttccg ttttccactt tattccgcct gaaatcaatg
     1861 accgacatca caccgtttgc caaccgcttg ggcaaaaaca tcaaacacct tatgaaatgg
     1921 gcgaaacgca acggcatcga agcctggcgc atttacgacc gcgacatccc ccaattccct
     1981 tttgccgccg atgtttacgg cgatcgaatc caccttcagg aatacgatac cggctggctg
     2041 atgcggcccg gagaatacga agcgtggctt gccgaagtat tggaagccgt cgctttcgtt
     2101 accggttttg aacccgaaca aatccgtctc aaacgccgcg aacgccaaaa aggtttgcag
     2161 cagtacgaga aaaccggcaa ggcgggcgac gatttcgtca ttgccgaaaa cggccgcaag
     2221 ttttgggtca acctcgacaa atacctcgac accggcttct tcctcgatca ccgcaacacg
     2281 cgcaaaaaag tcggcgaaac ggcggcgggc aaacgttttt tgaacctgtt ttcctacacc
     2341 ggcagcttca ccgtctatgc cgccaccggc ggcgcggcat ccagcgaaac cgtcgatttg
     2401 tccaacactt atctcgattg ggcgaggcgc aattttgaat tgaacggcat cgatatggaa
     2461 cggcacaaaa tcgtccgcgc cgacgtgttc caataccttc agacggcata tggggaaggc
     2521 aggcagttcg acctcattgt gatggacccg cccagttttt ccaacagcaa gaaaatgccc
     2581 ggcatcctcg acatccagcg cgaccatcaa aaactcatag acggcgcggt gaacctgctc
     2641 gcttcagacg gcattttgta tttttccaac aacctgcgca gcttcgtttt ggacgattcg
     2701 gtatcggaac aatacgccgt gaaagacatt tccaaacaat ccgttcccga agatttccgc
     2761 aacaaaaaaa tccaccggtg ttgggaaatc cgccacaaat cttaagggtg cgaaaaagcc
     2821 gccggaatct tccggcggct tttcgattgc actttatcag gcgatggagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacta ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gaggtcggat ttcaaaacat cttgctcgaa ggcggcatcg ccggtgtgta cgattaattc
     3181 gctgctcatt atttttcctt ttcggttggg attcgcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tttgaaaatt acttttatta
     3361 tcaaagtata aggaatgttt ttgaggctgt acagaaacgc gcgggcggtg tatgctccgt
     3421 ttaaatagat tcttaaagta tgaatgttca aaaccaaacc cgaaacccta taaaaggaac
     3481 actgcaatga ctgatttttc cgtttgggaa gctgctccct ttggcgcaac cgttgatcat
     3541 atcctgcaac gctaccacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggcgg aaattgccgg gctgcttgcc
     3661 gatatgcggg acgagctgct gatgcatatg atgaaggaag agcggatgct gtttccgatg
     3721 attaatcagg gtgtcggacg cggtgcggcg atgcccatca gcgtgatgat gcacgaacat
     3781 gaagaacacg accgcgccat cgcacggctc aaagagctga ccggtaattt ccacgcaccc
     3841 gaaggggctt gcggaagttg gacgcgcctt tacgcgctgg caaaagaaat ggcggacgat
     3901 ttgaacgacc acattcattt ggaaaacgat attctgtttg cccgcgtact cgattcttaa
     3961 gggataggag tcggatattt caatgccgtc tgaaatacgg tttttccgtt tcagacggca
     4021 ttttcatgtt tcagcccagc aattcgggcg gaatgcggca cacaggaatc gggttgattt
     4081 tgtgctgcat cgcgcggtga agccgcgtat agatttccag aacttcgcgc cgccgtcctt
     4141 caaaatcttc gggtttgcgc gtgccgtaca cgcccattgc ccactccagt tcgggatagc
     4201 ttgcgcccat ctgttcctcg tcggtgcgtt ccgtatccca caggccgtcg gtcggcgggg
     4261 ctttttgaat cgcctcgtcc acgcccaatg cttcggcaag ccggtaaacc tgcgttttgg
     4321 tcaggtcggc aatcgggctg atgtccacgc cgccgtcgcc gtatttggta aaaaaaccca
     4381 cgccgaaatc ttcaacctta ttacctgtcc ccgtaaccag cagtccgtgt atctgcccgt
     4441 agtagtacag ggtcagcata cgcaggcggc ttctggcgtt ggcgagcgaa agcggctgat
     4501 tggcaaatgc cgtccgatga acgccgacgg tttgctcaaa ggtttggaag gtgtcggtca
     4561 gattggccgt ttgcgcgctt acattggcat attgcccttg cagattgcgg atgtgccgcc
     4621 ttgcccgctc gagctggccg gggtgttggc gtatcggcat atccagaagc agcgtggtgc
     4681 ggccggtgcg ggcggcgagc gtggagacga cggcggaatc gatgccgccg gaaacgccta
     4741 cgacgaaccc ttttgcattt gcctgagcgg cgtattcgtc caaccatcgg acaatatgtg
     4801 tgataactgc ctgcgtatcc atttttcctc ctgtcgaaaa tatccgcatc atagcctgcc
     4861 ggcggtgcaa tttgaataga ttgagggcgg gttgatgaat atttttccca ttccccgctt
     4921 aaaaaagtca tataggaaaa ggctggcggc aagatggcgc gtgttataat attgttgaca
     4981 atttagacgg cacgatgccg ccttgggctt caaaacatat tcatattttt tattatttga
     5041 ttgattcgat gtctgatttt tttcattctg acgtgttgtc cgtttccgag ttgaacgcat
     5101 ttgccaaaag tattttggaa aaccaccttg ccggcctgtg gattgcgggc gaagtgtcca
     5161 atctgacccg cgccgccagc gggcattatt atttctcact caaagacagc cgcgcgcagg
     5221 tgcgttgcgc gatgttcaag ggtgcggcgg cgcgtttggc gcaacctttg aaagaaggcg
     5281 accatatcga agtggcgggc aaaatcagta tttacgaggc gcggggcgaa tttcagatta
     5341 ccgtaaacga agtacggctc aaaggtttgg ggcagcttta cgaagcctac gagcggttga
     5401 aggcgcagtt gcaggcggaa ggcgcgtttg cggcggaacg caaaaaacct ttgcccgtcc
     5461 gtccgcaatg tatcggcatc gttaccagtc tggcggcggc ggctttgcgc gatgtcgtaa
     5521 ccaccttgaa acgccgcgcg cccgaaatcc ccgtcatcgt ttatccggcc gccgttcagg
     5581 gcgcgggcag cgggtttcag attgcccaag cgattaaaac cgcatcgcaa cgcgccgaat
     5641 gcgatgtgct gattgtctgt cgcggcggcg gcagcatcga agacttgcgg gcgtttaacg
     5701 aagaaccggt cgtgcgcgcc atcgaagcct gcacgattcc cgtcgtcagc ggcgtgggac
     5761 acgaaaccga tttcacgctc gccgatttcg ttgccgacgt gcgcgcgccc acgccgaccg
     5821 gcgcggcgga actggtcagc cccaaccgcc aagaatcact gcaccgcctc gtccaagccc
     5881 aaggccgtct gaaaaccgtt ttggagcaac gctatttcga tgccagccaa aaactcgact
     5941 ggctcgcgcg gcaaatccgc cacccgcgcc aaaaacttga cgagcagcgc gcttcaatcg
     6001 gcaagctggc gcaaacgctg tcttactcga tgacgcaaaa cctccgcgcc cacaccgccc
     6061 gttttgaacg ccaaacccaa gccctgcaac attgccgtcc tgatgtttcc gtttaccggc
     6121 aagatatcgt ccgccttcag acggcattgc ccgccgcctt cagccggctg ctcgcccgtc
     6181 gccgtcaaag cctgaccgcc caagccgcat tgctcgaagc cgtttcgccg cagcacattc
     6241 tggaacgcgg cttctccgtt gtcaaaaata cacgcggaca agtcatccgt aacgctgatg
     6301 ttttgaagca ggggcagaag ctgcacatta ccttctccga cggcgaaacc gacgtgcgcg
     6361 taagcaaaga acaagggcag caggatttgt tcgattgcat ttaaacccaa tcggaaaaac
     6421 cgctccccct ccgccgtcat tcccgcgaaa gcgggaatcc agtcccttga ccttaagata
     6481 atctttaatt cgtcattcct gcaaaagcgg gaatccggaa gcggaaagcc gccggggttt
     6541 atcagaaatg actgaaactc aaaaaacgga ttcccgcctg cgcgggaatg acgattgata
     6601 cgtttcgata atttattgaa gatggctgaa attcaaagtt acagaaattt ataaacaaaa
     6661 aaacagcaac ctgccgccgt cattcccgcg aaaacgggaa tccagtccct aaaaagcggg
     6721 aatccggaag cggaaagccg caggaatttg tcggaaaaaa cagaaacctc tccgcacacc
     6781 cgaaatgttt cagacggcgc atcttggggc ggataaaaac ccaacccgat atttttttat
     6841 aatttttaat aagtttccat tgggcaaatg aaattttatt tttcaactga cagaaagagg
     6901 atgttatgaa gtttttagac cgtgaggcaa gcattgccaa gccgggtttc aaccgttggc
     6961 tcgtgccgcc cgcagcatta gcggtgcacc ttgccatcgg gcagatttac gcctattccg
     7021 tattcaacgc gccgctgacc aaacttatcg gcataaccga atcggcggcc ggagattgga
     7081 agctgacgac cgtaggttgg attttcagta tcgcactcgc gatgctgggc gcgtcggccg
     7141 ccctgttcgg cacatggatg gaacgggtag ggccgcgcaa agccatattt gccgccgcct
     7201 gctgtttcag cctgggcttc ttcgtatccg cattcggcgt gcgtacgcac aacctcttcc
     7261 tgctttattt gggcaacggc gtcatcggcg gcgtaggctt aggcttaggc tacatcgggc
     7321 cggtttccac gctgatgaaa tggtttcccg acaagccggg tatggcgacc ggtttggcga
     7381 ttatgggttt cggcggcggc gcaatgctgg cctcgccgct ttccgtatca ctgatgaacg
     7441 ccttttcaaa cgccgcttcg gtcggggttg ccgaaacctt tgccgtattg ggcctgtttt
     7501 acctcgcact gatgatgttc ggcgcgttta ccatccgcgt acctgccgac ggctggaaac
     7561 ctgaaggcta taccgtgccg aaaacgcaaa acaagccggt cagcagcaat catgtcaacg
     7621 tgtcccaagc catgaaaacg ccgcagtttt ggctgttgtt ctgggtattg tgcctgaacg
     7681 taaccgccgg catcggcgta ttgggccagg catccgtgat gattcaggaa ctcttttccg
     7741 aaacctccgc aggcaggcag gcggcagtcg gcgcaggtgc ggcggcaggc ttcgtcagcc
     7801 tcctgagcct gtttaatatg ggcggacgct ttttgtggtc gagcgtttcc gacaaaatcg
     7861 gacgcaaaaa cacctacacc atcttcttcg tactcggttc gctgctgtat ttcgccgttc
     7921 cctccatcgg cgaaggcggc agcaaagccc tgttcatcat cggcttctgc gtgattattt
     7981 ccatgtacgg cggcggattt gccgccattc ccgcctacct gaaagacctg ttcggcacct
     8041 atcaggtcgg cgcgattcac gggcgcatcc tgctggcctg gtcaaccgcc gccgtcatcg
     8101 gcccggtact ggtcaattac atacgccaaa gccaaatcga cagcggcata ccggcggcac
     8161 aggcatacag cgtaaccatg tacattatgg cgggattgtt gattgtcgga ctgttgtgca
     8221 acctcgccgt caaatccgta cacgaaaaac accacgaaaa agacatcaaa accgccgcac
     8281 gcagcggcaa ccccgacgac gaaaccgcca tatccgacgc ttaccttgtg ggcgaaaaag
     8341 tttccggcga cggcatatcg gtctggtggc gttgggcatt ggccgtcata ccgctcgcct
     8401 acggcgtggt gatggtcttt gtcaaagcac tcgacctttt ctcctgaaaa cgcccgaata
     8461 aaatgccgtc tgaaacagca ggtgtttcag acggcatttt tactttcagg cttttttgcg
     8521 gcggcgttcg cccggcagca gcatatccgc ccatttgagg atgttggcga cttcggcggg
     8581 gttgagttcg tagaactgtc cgcgtttgag gcggttgggc agtccgatgg ggccgaaggc
     8641 gacgcgcacg aggcggctga ctgtgaggcc ttggctttcg aatatgcggc gcacttcgcg
     8701 gttgcggcct tctttaatca cgatgttgta ccatttgttt gcgccttcgc cgccttgttc
     8761 gtagatgcgt tcgacttttg ccaagccgtc ttcgagcatc acgccttctt cggtgagact
     8821 gcgcatttgt tcggtagtca gcccgcccag tacgcgcacg gcgtattcgc gttcgacttc
     8881 gaagctgggg tgggcgaaac gttggacgag ttcgccggag gtagtgagaa tcagaagtcc
     8941 gctggtgttg atgtccaagc gtccgatggc gacccagcgg ctgctggcgg cctgcggcag
     9001 gcggtcgaat atgctgacgc ggccttgcgg gtcgtcgcgg gaaacgattt cgccttcttg
     9061 tttgtaatac aggatgatgc gcggcaggcg gtcggcccat ttgagtttga tgatgctgcc
     9121 tttgacggta acgtggtcgt cgggggtaac tttgtcgccc agttgcgcgg ttttgccgtt
     9181 gaccgttacc cagccgttgt tgatccattc ttccatttcg cggcgcgagc cgacgccgga
     9241 ggctgcaagc actttttgca ggcgttcggg ttccatacgc gacaggtcgc tgcggcgttc
     9301 tttcaaatcg cgcgcgtgtt ccataatttt ttggttggga ttgcggacga cgagtttttt
     9361 ggctttggcg gcgcgctgtt tgggggcgtt ttgcagcttg atgccgtctg aagctttttg
     9421 tccgtaagct tgggcggcgg ttttgcgcgt ttcgtctttg ggacgggctt tgcttttgaa
     9481 aggtttggcg gttttcttgg cagacggggc tgcgccgtcg cgccattggc gtttgctggt
     9541 gggttgcttg gacatcattt ttcctaactc gcctgataag gcggttcttc tgcggcgtgt
     9601 gcggcaggac gggttgataa aatgccgtcc tcggtcggac ggttcgatat tgtacctgat
     9661 gcggcggttt tgcggatgaa atcgtcgctg cggctttggg gacaaagttt gtgcaacggt
     9721 tgaaatcggt ttgcgtgcgg gagtagaatc tttcaaatga taagaggagc ggtaaatgga
     9781 tttgcacaat attcgggaag attacagcaa acgggaattg tcggaagccg attgcgccga
     9841 taatccgatc gagcagttcg agcggtggtt ggacgaggcg gtacgcgccg aggtcaacga
     9901 gccgacggcg gtcaatgtgg cggcggtcga cgggcgcggg aggcccaaca gccgtatggt
     9961 gcttttgaag gaggttaatt cagaaggttt tgttttcttt acgaattacc acagccgcaa
    10021 ggggcgttcg ctggaactca atccgtttgc ggcgatgacg tttttttggc cggagctgga
    10081 gcgtcaggtg cgcgtggaag ggcgtgtcgg aaggctggcg gagaagttgt cggacgaata
    10141 ttttgagagt cgcccgtatc agagccgttt gggtgcgtgg gcaagcgcgc agagcgaggt
    10201 gattccgaac aaggcggtat tggtggcaaa ggcggcggcg gtcggactca aacatccttt
    10261 gcacgtgccg cgtccgccgc attggggcgg ctatattgtg attcccgatt tgattgagtt
    10321 ttggcagggc aggccgagca ggctgcacga ccgcatccaa taccgtttgc tggatggcgg
    10381 ctggatacgc gagcggttgt cgccttgaac aggaaaaaac atatgccgtc tgaaaaccgg
    10441 aatcaggttt cagacggcat tttttcacgg ggaaaatacg gacgggaagt tttttgtgtt
    10501 ccgaacggaa agcccgaatc ctctttatac cgaggaatta tgatgcggac ggcgtgaatg
    10561 cgtcggagaa aaacaagttt tccggcagct tatgctgttg cacaaacaga ttttttgctt
    10621 gttcggtcat agccggagag ccgcaggcaa acacttcgta ttccgacagg tcggggtggt
    10681 cttgtgccgc gatgtcttgt acgtggcctt ttcttccctg ccagccctct ccgggacggg
    10741 acaatacggg ggtgaagcag gtgtttttca gacggcatgc caacccttgt gcttcttcga
    10801 gggcatacaa atcatcttga tgacgcgcgc cccagtagaa atgggcagcg cggctgctgt
    10861 tttggcggat aaggtcgagc aggatgctgc ggatgggggc gtagcctgtg tcggttgccg
    10921 gcaggatgac gggtttgccg ctgtcttcct gcaaggtaaa cgaaccgagc gggcctttaa
    10981 cgcggacgat gcctttttct ttgactttgg gttcgctgcc gaaaatcatt tccgagcaga
    11041 caccgttttc gcgcctgcgg atgtgtagtt ccaaaatgcc ttcttggtcg ggcgaattgg
    11101 cgatggagta gctgcggctg acgttgcccg gcagcagtaa atcaatgtat tgcccggcgt
    11161 agaaggcaaa cggcggggct ttgggcaggg caagtttcag gagggcgaca tcgtgtttga
    11221 aaaccatact ttcgatgcgt gcgggcaagg tgcggatggg tagggcatcg gctttgcagc
    11281 cggggatgtt gatgttgata tcgctttgcg cggtggtgcg gcacatcaaa atcttgcctt
    11341 gcgctttttc tgcttcggat aaagcctgtt ccgagtgtcc gcccatttga atatcgccgc
    11401 tggccagttc ggctttgcat tgtccgcaga caccgttttt gcaggaatgg ggcagattga
    11461 ggttttgacg ggcggcagcg gacaaaacgg tttcgccgtc gttggcggca aaggtggttt
    11521 ggtcgggcag ggtaacggtg tggttcatcg tatttccttt tcggttgaaa ccccaccctt
    11581 taggccgata ggatcagact ttatttggga ggggcgtaac ccctttcaaa tcagggcaac
    11641 acacagggcg acattttatg tgtcgtcctg tgtgttgaaa cgtggaatat tgttttttat
    11701 gctgttttaa tgtggtcaga ctgtgaaaat gccgtctgaa cgtcggattt gggtttcaga
    11761 cggcatcttg cttatcaggc aagtttggct ttaatcaact cctgcacctg agcgggattg
    11821 gctttgcctt tgctggtctt catcacttgc cccacaatcg cattcagggc tttttcgttg
    11881 ccggatttaa actgttccac ggctttggcg ttgtttgcca gcacttcgtc caccatcgct
    11941 tcaaccgcgc cggtatcggt catctgttgc aaactgtgtt tttcgatgat ttcggcaatg
    12001 ctggtttccg gttccgccca catggcttca aaggcttttt tcgccagttt gccgcttaat
    12061 gtgccatcgg caattttgcc caccagcgcg gcaaggcgtg gggcggtaat cgggctgtcg
    12121 gcaagttcca tgccttcttt gttcagcgta gcggcaagtt cgccgttcat ccagttggca
    12181 gtcggcttgc cttgtccgct ctctttggcg gcttcttcaa aataggcagc ctgcacgcgg
    12241 ctggcggtga gcaggcgcgc gtcgtattcg gacacgccgt aatccgccac gaaacgcgcc
    12301 gccatttctt tcggcagctc gggcatttct gctttggctt tttgcatttg ggcatctgaa
    12361 atgatgacgg gcagcaaatc gggatcgggg aagtagccgt agtcgtgcgc gtcttctttc
    12421 aggcgcatca cgcgggtttc gcctttttcc gggtcgaaca gcatcgtcgc ctgctgaact
    12481 gtgccgccgt cttccaaaat ctcgatttgc gcttcggctt cgtaattaat cgcctgatcc
    12541 aagaaacgga aggaattgag gtttttaatt tcgcggcgcg tgccgaattc cgcttggcct
    12601 ttcgggcgaa cggatacgtt ggcatcgatg cggaacgaac cttccgccat attgccgtcg
    12661 caaatgtcca gccaggttac caagctgtgt aaggctttgg cgtaggcaac ggcttcggcg
    12721 gcggagcgca tttcgggttc ggataccact tccaacagcg gcgtgccggc gcggttcagg
    12781 tcgatgccgg tcgcgccgtt caagccctcg tgcacggatt tgcctgcgtc ttcttccata
    12841 tgggcgcggg ttacgttgat ggttttcaca tcgccgccga ccacgatttc caatttgccg
    12901 tgttcgacaa tcggcaaatc caactggctg atttgatagc ctttcggcaa gtcgggatag
    12961 aagtagtttt tgcggtcgaa cacgtttttc cggttgattt ttgcatccaa agccaaacct
    13021 aatttgatgg ctttttcaac gacttcgcgg ttcattaccg gcagtacgcc cggcagcgcg
    13081 cattccacta cgctggcgtg cgcgttgggt tctgcgccga atgcggtcga tgcaccgctg
    13141 aagattttgg atttggtgtt caattggacg tggatttcca agccgattac agtttcccaa
    13201 gtcataggag gttctttctc taaaatatca attcgatagt cttcgcatca gactgtccgg
    13261 cggagcggtt tttccgtttg gatgccgtct gaaatgccgc gtgcttctgt tttcagactg
    13321 tccttcatct ttgtaaccat atcgtccagc atttcataac gcttgcggta catcgaacgc
    13381 tttttgcttt cgatgtcggc aaggtctttg cgggcggggg tttggggaag atgccagtag
    13441 ccgtcccgtt ccaaacttgc gccgtattcc tgccagaatg cgtcgtaatt cattttgacg
    13501 cgctttttaa gtttccagcg taatttgact tgatgttttt gtgcaatgcc tatcgcgccg
    13561 tctaatttta atgcggcggc gaaatattgc agtgcggtta ccatcagttg ttggggacgt
    13621 aagccgtgaa gttgtttggt tatgcggcgg acggtgtctt tggcttcttc acctgacggc
    13681 ccttgtacgg aggctgtcag caggtgtgtg ccgacaaagg cgaaggtcgc catatacagc
    13741 cggttgcctg cttcatcgcg caaagataaa gaccacgcgc cttcttcgac gcagttgtcg
    13801 ttgcggttga gccacaagct gatgccgtct gaaaggtgcg ccaagaccag atggaagcgg
    13861 cagtcttcca tttggcacag aacgtctgtg ccgaagagtt tttccgccat gagaaaatct
    13921 gccgtcatcg catctaaacg gcgtttgcag ttaaaacgcc tgtcaacaaa ttcgcgcagc
    13981 agcggatagg catcctgcgg gcagtggagg aacagcccct gacggatagg ggaggcgttg
    14041 acaaactgct cgaaggcgta aatttctttt tttccgaaaa attttcgcaa tgtgtatttt
    14101 aaatgttgca gccgatataa aggcgcatag ctgtaaacgg tgcgaaaatc ggggaaggta
    14161 aaggtgcggt tctgcttcat tcgggtcgtt tgccgtgcca atcgctgttg agttggattt
    14221 gatgcgccgc accgaggatt ttggcttcgg cgaagtagtt gccgacaagc tgtacaccga
    14281 cgggcagtcc gccgccgctg aagcctgcgg gcagggtcaa tgcgggcagt ccggcgaggt
    14341 tgacggcgat ggtgtaaatg tcgctcaagt aggtttcaac cggagaagta tccgctccga
    14401 ttttgggtgc ggcggagggt gcggtcggcg cgaggatgag gtcgcaccgt gcaaatgccg
    14461 tctgaaagtc atcggcaacg aggcggcgca gtttttgggc tttgagataa taggcatcgt
    14521 agtagccgtg cgacagtacg taagtgccga tcatgatgcg gcgtttgact tcgctgccaa
    14581 aaccttcggc gcgggttttg ccgtacattt cttccaaatc gccgaattgg gcggcacggt
    14641 gtccgtaacg tacgccgtcg taacgtgaaa ggttggtgcc ggcttctgcg gaggcgagga
    14701 cgtagtaggc ggggatggac agcttggttt gcggcaggga aacttcgacc ggttccgcgc
    14761 cttgtgcttt cagcaaatca atggtgtttt gcaatgccgt ctgaacatcg gtgctgttgc
    14821 cttcgctgaa gtattctttg ggcaggccga ttttcacgcc tttgagcggt ttgtccaaat
    14881 cgcgggtgta gtcttctttt tcgcgttcga agctggtgga gtctttgggg tcgaaacctg
    14941 ccattgcatt caacagaatc gcgcagtctt cggcggtttg cgccatcggg ccggcttgat
    15001 cgaagctgga ggcgtaggcg accataccga agcgggaaac cgtgccgtag gtaggtttga
    15061 tgccggtaat gccgcagtgc gatgcgggtt ggcggataga gccgcccgtg tccgaaccga
    15121 gcgcggcagg ggcgaggcgc gcggcaacga cggcggcgga accgcctgac gaaccgccgg
    15181 gtacgtgttc cggattccac gggtttttgg ccgcgccgta gaatgagttt tcattggtcg
    15241 aacccatagc gaactcgtcc atgttggtgc ggccgagcgt taccatacct tcgtcgagca
    15301 ggttttggac gacggtggcc gtgtaggggg agacgaagtt gtcgagcatt ttggacgcgc
    15361 acgcgctgcg ccagccggtt tggcagaaaa tgtctttgta ggcgacggga acgccggtaa
    15421 gtgccgaagc gttgccctgt gcgatgcgtt cgtcggcggc acgggcttcg gcgagggttt
    15481 tatcttggtc gatggtgatg tagccgttga gggcgggatt tttttcggca atggcggcaa
    15541 ggtatgcgct tgccagttcg acggcggaaa tctgtttgga ctgcaacagg ctgcctgcct
    15601 gtttcaatgt gtattgggtc ataaatcttc catccttaaa gggcttcggg cggtattgct
    15661 gatgccgtct gaagcgtatt cggattattc ttcgataact tgcggtacga tgtacaggcg
    15721 gttgcgtact tccggcgcaa cggcttggta ttcggcggcg cggtcggttt cggttacttc
    15781 gtcttcacgc aggcggaggg cgacctcgtg cgggtgcgcc atcggctcga tgccgtctgt
    15841 gttgatgttc tgcatctgtt cgaccatagt gaaaatgtcg tttaattctt gaagcgattt
    15901 ttctttttct tccgcagtca gctgcagtcg ggagagtcgg gcgattttgt ctacgtcggc
    15961 aagtgtcagt gccatgaaaa gcatccttgt ctgatggtaa agataccgtc gttcgggtat
    16021 gatggggcgt attgcttgga tttttaatta taacggaaaa atccgttgcg gcggcaattt
    16081 gcagcatcgg atgcctgccg cacgggtttg aaagagggga aataatggga ttgggtatgg
    16141 aaatcggcaa gctgattgtg gcttttttgg tgctgatcaa tccgtttagc gcgttgtcgc
    16201 tttaccttga cctgaccaac ggacacagca cgaaggagcg caggaaggtc gcgcggacgg
    16261 ccgccgtcgc cgtgtttgcc gtgattgcgg tatttgcgct gatcggcggt gcgctattga
    16321 aggttttggg catcagcgtc ggttcgtttc aggtcggcgg cgggattttg gtgctgctga
    16381 tcgccatttc gatgatgaac ggcaacgaca atcccgccaa gcagaatctc ggcgcgcagc
    16441 cggaaacggg gcaagcgcgc cccgcccgca atgcaggggc gattgccgtc gtgcccatcg
    16501 ccataccgat caccatcggt ccgggcggta tttcgaccgt gattatttac gcttcggcag
    16561 ccaaaacgta cagcgatatc gcgctgatta tcgcggccgg tttggtggtc agtgcgattt
    16621 gttatgccat tttaatcgtt gccgggaagg tcagccgcct gctgggcgcg acggggctga
    16681 cgattttaaa ccgcattatg ggtatgatgc tggcggcggt atcggtggag attattgtgt
    16741 cgggactgaa aacgatattc ccgcaactgg caggttgatt cgatgtttca cagtgtcaat
    16801 gtatttacgg gcgaaacgct ttaccgccgc cccgatcaag attatgcgga gtttgaacgg
    16861 cggctggcgg atttgaaaat gcacggcagg gcgttcgcgc aattgggcgt aaccgaacgt
    16921 gccgcgcgtc tgcaaaaatt tgccggccgt ttggaggcgg aaaaagagcg ttttgcggaa
    16981 atggtgtgcg aagaagtcgg acgctgcctg cacgaatgcc gtgccgaaat cgtcaagtct
    17041 atcgaactga tacgctatta cgcccgcctc gcccccgaac tgctcgccca caaaaccatt
    17101 gcgacgcagg cgagtttgag tcaggtgcgc ttcgagcctt tgggcgtggt gtttgccgtc
    17161 atgccttgga actatcccgt ctggcaggta ttgcgttttg ccgttcccgc tatgtgcgcg
    17221 ggcaacgcgt gcgccgtcaa acccgcgccc agcgtggcac gcgtcagtcg ggcgttgttc
    17281 gatttggctt cagacggtat tcctctggca ggcgcgtggc tggatgaggc ggacacgctc
    17341 aaggcagtcg aagataccga tgcgatggcg tttaccggtt cgacgcatac ggggcgcatt
    17401 cttgcggcac atgcgggcgc aaacctcaag aaaaccgtgt tggagctcgg cggcagcaac
    17461 gcctttatcg tgatgcccga tgccgatttg gaacgcgccg ccgcagaagc ctgttattcc
    17521 cgtttccgcg atgcggggca atcgtgtaac gccgccaagc gcatcatcgt aaccgaagcc
    17581 gcagtcgacc gctttattca gctgtttctc gccgaatgcg ccaaattgaa aatgggcgac
    17641 ccgaaacatc ccgataccac gcttgcaccg ctgcaccgcg aagatttgcg ggaccgggtt
    17701 cacgggcagg ttgaagatgc cgtttcaaac ggcgcggtgt gcctgaccgg aggcaaagtt
    17761 ccacaggggt cggggtggtt ttaccctgcg acggttttag acagggtaaa tcctgcctgc
    17821 cgcgtctgga gagaagaagt attcggcccc gtcgccctga ttttgcgcgc cgaaaacgaa
    17881 gaacacgcca tctgccttgc caatgattcc ccgttcgggc tcggtgcctg catttatacc
    17941 gccgatactg aacgcgcctg gcggtttgcc gaaaaaatac aggccggatc ggtgtttatc
    18001 aaccgccata ccagcagcga tttgcgcctg cctttcggcg gagtcaaaga ttccggttac
    18061 gggcgcgaac tgtccgaatt cgggttgtac gagttcgtca acgtcaaaac ctactggcag
    18121 aaataaacaa accccgtgcc gcttcaggcg gcatcgggtt tgcgctagag ccgattaacg
    18181 gcagtatttg tttacggcgt tattgtattt acggatcaaa tcatccttgt ttttcgcatt
    18241 tgagtttccc accgccttca ggttcatttt tgaaatccgg cagttttctt ctttgttctg
    18301 ccgttcggtt tcggcaattt ttttcttttc ttcctcaagc tgcccgtttt tctcggcgat
    18361 atccttttcg ttttccttcg cactgtccgc attcgtatcg acggcaggtt tgggcttgac
    18421 cgccggtttg gtttggagcg tccgcaggtt gaggatttgg ctctggtcgg gatgaagctg
    18481 tttcggcaca tccgaatagc tgttgccgcc gccgtctttc caagtaaaga cggcggttcc
    18541 ggcaggcggg acgggcagga aagaggcgag ggtaaatgtg atgacggata aagcaaagtt
    18601 cattagggtt tccttgatgg ctgactgccc gggcagaatc gggattggtt cgggcggtgt
    18661 cttttccggg ttcgggtcaa ggcagggaag acactccgcc ttaatatggt gctttatata
    18721 ttggggtaaa acagattccc aaaaatatca ttggaatatg gttatgctac cgtatatttt
    18781 ggcaaagaaa aagaaaattg caggcaggcg cgtataaaat ccgatgaggc gcgtgattct
    18841 gcctttggtg ttgaatcggt tgaatctgcg gtttttgata tttttaattg atgaaatgcc
    18901 gtccgaacgc tttgaaaagg gttctgacgg cattttgttc aaaccggttt atttgcgttg
    18961 caatacggcg gcgaagaagc cgtcggtttg gtgtcgggcg gaatcgaggc gcaggtattt
    19021 gccggtatcc aaatcgacct tcaggttttg cagcagttcg gcgcagttga cgggttcaaa
    19081 ttcgggatgt tcggacagga aacgtttgat ttgcagttcg ttttcttccg gcaacacgct
    19141 gcaggtggcg taaaccaaac agccttgcgg tttcaccagt ttggaggcgg catcgaggat
    19201 actgtgttgc tgttccaaaa gtttggcgac ggtctcggcg gattggcggt atttgaggtc
    19261 gggattgcgg cgcagtgtgc ctaagccgga gcagggcgca tcgaccaaca cgcggtcggc
    19321 tttgcctgcc agtcgtgcga tacgggtatc gtgttcgctg ccgatacgtt cggggtggat
    19381 gttggtcagt ccggcacggg tcatgcgcgg tttgaggttg gcaaggcgtt tttcggcgat
    19441 gtcgaaggcg tagattctgc ctttgttggc catttgggcg ccgacagcca aggttttacc
    19501 gccggctccg gcacagaaat ccacaatgat ttcgccgcgt tttgcgccca ccaataaggc
    19561 aagcagctgg ctgccttcgc cttggacttc cagcgtgccg tccaaaaaca gttcgtgttt
    19621 gttaagcgcg attttgtttt tcaggcggat gccccaagga gaataaggcg ttgcctctgc
    19681 atcggggctt tcggcttgca acaatggcaa tactttgtcg cgtttgcctt tcaaggtatt
    19741 gacgcggatg tcgagcggcg cagcttggtt gatgctgcgg ccgaaagcga ggatttcttc
    19801 ttcgctccaa tgctgttgca gttgttccac cagccattgc ggcaattctg cggcggtatt
    19861 caggccgtct gaaaactcgg ttttacgggc tttcaaattg ccgaaaaact ctgtttcttc
    19921 ttcatcaagc aggtctttga tttggctgat gttggtactt ctgccgagaa ccagcgcggc
    19981 gagtgcggct ttgcgcggct gcgcgtgcgg acggcgcagg gcggtactga ttttttgata
    20041 gtgtcgcaac gcggcaaagg cggtttcggc gatttcgtgg cggtcttggc ggccgagctt
    20101 tttgtgtttg cggaaatagg cggagaggac ggcatcggca ggctgtttga aagtcagcat
    20161 ttcagccaaa actttggcgg tatggtcgag ttgtgcggcg ttcataatgt aatgggttct
    20221 cttaaagttg gtaaagggtt tcagacggcc ttcataatac gttcgatttc gtgccaaaag
    20281 ccgtccggcc gacattccaa aacggcaatc aagccttggt cgatgcggcg gatttcggtt
    20341 gcgtttaatg tttcggtttc ggcgacacgc gcaggcgcga gatagaccat gcggtcgagc
    20401 aggtgatagc gcgcttcttg gcgttcaaac ccgtattccg cccaatcttg aatatagatg
    20461 ccgcgccagc cgtatggatt aagcggcggc tcaaaagcat ggaaaccttg tggaaaaaat
    20521 ccgatgccgc gcacgatgga agcaggaagt ggattttccg gcaggtcttg tgcggctaag
    20581 gttgctttgc cttgcgacgt atgcaccagc tggaattgct gcaccaattt ggcggctttg
    20641 tccgtcagcg tgtctttggg attgagaccg cgcaggttct gcacttggtc tccgccgtag
    20701 tatttacacg ccagctcgat atgatagggc tgttggttga gggaaacgac aaaatccgcg
    20761 gcgcctaaag tttggccgtc tgaaaaaacg gtcagattat atgcgtgcag tttggtgtgc
    20821 ggtgcgttgg taaaccaaaa agccagcagc tcttcggcat aaatgccgag acggtggtcg
    20881 aacggggcgc gttgtgcaag gtaatccgtc agcggcgcgg gatcggcatc caatgctaaa
    20941 aggtagcgga aaccgcgttc ccccagtagt tcgcgcacgc tcaattcgca accgctttgc
    21001 cacaaaggcg gcgcagtcag cagcgaggcg aggtcgcgga cgggttggct ggtgagtttc
    21061 caccataatg cgtctagggc gtaattcatg cttatccgaa tatcagtcat gccgtctgaa
    21121 aaagcttgta atgttttcag acggccttaa tcagtctttt ttatggatac ggtggctgac
    21181 ggtattgaaa aaaccgcgca ggatgtcgat gtcttcggtt tgcgtgttgg cgcgtccgaa
    21241 caggctctgc atacggcgca tcagacgctc gccgttgcgg cggttgaaaa agccgatgtc
    21301 gtccatcacg ctttccatgt gggcgagcat gcctttgatt tgctcgtggg ttgcggcgtg
    21361 gtcttcctgt tgaagatggg tcatgggcga atcggtttgg ctgaaaattt cgtagcacac
    21421 gacctgcacg gcttgggcga ggttgagcga gaaatagtcg gggttgccgt tgatggtcat
    21481 cagtcggttg cacgctcgga cttcttcgat gctcaagccg aaagtctcgt tgccgaagac
    21541 cagcgccact ttctcgccgc ggttggcggc ctgcagtaat tcgggcacca aatcgcgcgg
    21601 ggtttgcagc ggcgcggtaa tttcgcggcg gcggctggtc agggcgcagg cgatggtggt
    21661 gtcggcaagg gcttcgtcca gtgtggcgac gatttcggcg ttgtgcaata cgtccgccgc
    21721 gccggaagcg aggatgaaac tttcttcagg cagtgcaaaa ctttgcacgt catcgggatt
    21781 aaaaacgggc ggattttccg tcatcggcgt tgccatcaga ttgggggtga cgatggtcag
    21841 cctgtgcaga cccatcgttt tcatcgcgcg tgcggcagag ccgatgttgg cggggtggct
    21901 ggtgcgtgtg aggatgatgc ggatgttgcc gaggtagtcg ggcagggcgg gtttgagagc
    21961 agtcatgttc ggttttcggt cggaatggaa tagggtataa tgcgccgtct ttttcgttcg
    22021 gacggtgaaa tgccgtttga actgttcttt aacaacgtcg gaaatttaac gttgcgcctg
    22081 aagcagacac gcgtcaggcg gtattttacc cgatttggca gggtttgccc caaccagata
    22141 aactccgatt ttgccgctcc gaaggactgc acgtccgaac cggcggtatg ccacccttaa
    22201 ggaaatctga tgaatccgtt tttgaataca gcctttaaag ccgcccgccg tgccggtcag
    22261 atgatgattc gcgccgcagg taacctcgat gccgtcaaaa ccgacagcaa agccttcaac
    22321 gattttgttt ccgatgttga ccgcaattcc gaaatcatcc tggttgaggc tttgaaagaa
    22381 gcctatccgc atcacaaaat cacttgcgaa gaaagcggct tccacggcaa agctacagcc
    22441 gagtacgaat ggattatcga tccgctcgac gggacgacca atttccttca cggccatccc
    22501 caatacgcca tctctatggc actgctgcac aaaggcgtgt tgcaggaagc tttggtgtac
    22561 tcccccgaac gtaacgacgt gtacatggct tcgcgcggta aaggcgcgtt gcttaacgac
    22621 cgccgcatcc gtgtctccaa ccgcatcgaa ttgaaccgct gcctgatcgg taccggtttc
    22681 cctgttgtcg atcaaagcat gatggacaaa tatctggcga ttttgaaaga ctttttggca
    22741 aaaaccgccg gaggccgccg cgaaggcgcg gcttctttgg atttgtgcgc cgtagcaaca
    22801 ggacgttttg acggcttttt cgagttcaac ctcaaaccgt gggacattgc cgccggtgca
    22861 ttgattgtcc aagaagcagg cggcatcgtt accgatatgt cgggtgagga cgcttggttg
    22921 gaaagcggcg atattgtggc tgccaatccc aaagtactgg cgcaaatgtt aaaaatcatt
    22981 tccgcacacg tttaaatttc agccagctcc cggaaatgcg ggaaaacggg cgggtatgcc
    23041 gtctgaagta tcgtgccgat atttcagacg gcatttttat tggcggcggg gcaggaaggc
    23101 ggaaacggtc aaatgcccga aattgccggg aatggaaatc cgtatctggt taaacggaga
    23161 accctgtgca atttgtccct tttctttcaa ttgtttaaag gcggtttgct ggaatctaaa
    23221 tcggctgtgg cagaaaacag acaccgggga a
//
LOCUS       DAXDZG010000028        23225 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXDZG010000028 DAXDZG010000000
VERSION     DAXDZG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23225
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.028"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            221..6295
                     /locus_tag="ACFB78_000967"
                     /pseudo
     CDS             221..6295
                     /locus_tag="ACFB78_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            6513..8255
                     /locus_tag="ACFB78_000968"
                     /pseudo
     CDS             6513..8255
                     /locus_tag="ACFB78_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            8273..8641
                     /locus_tag="ACFB78_000969"
     CDS             8273..8641
                     /locus_tag="ACFB78_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268986.1"
                     /translation="MYDKMHKSCRFFAGSFCFMFGAVPDNRVKFPLAHYFKIEILYFI
                     SLLLKRCSHFFIGFIIHHLIYGFWRQGLTAFAAVIDFWDRGNAFKESKAIRPDADGGA
                     IIHLLEFVAIGADSCDDYVE"
     gene            complement(9017..10057)
                     /gene="mutY"
                     /locus_tag="ACFB78_000970"
     CDS             complement(9017..10057)
                     /gene="mutY"
                     /locus_tag="ACFB78_000970"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC1268987.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            10220..11260
                     /locus_tag="ACFB78_000971"
     CDS             10220..11260
                     /locus_tag="ACFB78_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC1268988.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            11358..11470
                     /locus_tag="ACFB78_000972"
                     /pseudo
     CDS             11358..11470
                     /locus_tag="ACFB78_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            11481..11666
                     /locus_tag="ACFB78_000973"
     CDS             11481..11666
                     /locus_tag="ACFB78_000973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268989.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(11855..12493)
                     /locus_tag="ACFB78_000974"
     CDS             complement(11855..12493)
                     /locus_tag="ACFB78_000974"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC1268990.1"
                     /translation="MSKLTPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(12702..14537)
                     /gene="edd"
                     /locus_tag="ACFB78_000975"
     CDS             complement(12702..14537)
                     /gene="edd"
                     /locus_tag="ACFB78_000975"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC1268991.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            complement(14621..14739)
                     /locus_tag="ACFB78_000976"
                     /pseudo
     CDS             complement(14621..14739)
                     /locus_tag="ACFB78_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15056..16501
                     /gene="zwf"
                     /locus_tag="ACFB78_000977"
     CDS             15056..16501
                     /gene="zwf"
                     /locus_tag="ACFB78_000977"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC1268992.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            16652..17347
                     /gene="pgl"
                     /locus_tag="ACFB78_000978"
     CDS             16652..17347
                     /gene="pgl"
                     /locus_tag="ACFB78_000978"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC1268993.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            17328..18314
                     /locus_tag="ACFB78_000979"
     CDS             17328..18314
                     /locus_tag="ACFB78_000979"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC1268994.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            18363..19211
                     /gene="hexR"
                     /locus_tag="ACFB78_000980"
     CDS             18363..19211
                     /gene="hexR"
                     /locus_tag="ACFB78_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC1268995.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            19437..21083
                     /gene="pgi"
                     /locus_tag="ACFB78_000981"
     CDS             19437..21083
                     /gene="pgi"
                     /locus_tag="ACFB78_000981"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC1268996.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            21861..22313
                     /locus_tag="ACFB78_000982"
     CDS             21861..22313
                     /locus_tag="ACFB78_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC1268997.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            22343..22498
                     /locus_tag="ACFB78_000983"
     CDS             22343..22498
                     /locus_tag="ACFB78_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1268998.1"
                     /translation="MAETVMPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKE
                     IQGYLID"
     gene            22603..22986
                     /locus_tag="ACFB78_000984"
     CDS             22603..22986
                     /locus_tag="ACFB78_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC1268999.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(23070..23204)
                     /locus_tag="ACFB78_000985"
                     /pseudo
     CDS             complement(23070..23204)
                     /locus_tag="ACFB78_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
BASE COUNT         6194 a         5757 c         6013 g         5261 t
ORIGIN      
        1 cctgcgacaa atagcccaat gagtttattt tgtttatacc ggcttagacg gcttttctca
       61 ttggcttatt ctaacctaaa tgggattttt agccgttatc cggtacgtcc cctaatttaa
      121 taaaaaaatg gcggtaattg acttaccaaa atctttgtcc tagaatgccc agactgtcca
      181 atgtttgtaa taactctata aaaatcaaat aactaaaatc atgaacaaaa ccctctatcg
      241 tgtgattttc aaccgcaaac gcggtgctgt ggtagctgtt gccgaaacca ccaagcgcga
      301 aggtaaaagc tgtgccgata gtggttcggg cagcgtttat gtgaaatccg tttctttcat
      361 tcctactcat tccaaagcct tttgtttttc tgcattaggc ttttctttat gtttggcttt
      421 gggtacggtc aatattgctt ttgctgacgg cattattact gataaagctg ctcctaaaac
      481 ccaacaagcc acgattctgc aaacaggtaa cggcataccg caagtcaata ttcaaacccc
      541 tacttcggca ggggtttctg ttaatcaata tgcccagttt gatgtgggta atcgcggggc
      601 gattttaaac aacagtcgca gcaacaccca aacacagcta ggcggttgga ttcaaggcaa
      661 tccttggttg acaaggggcg aagcacgtgt ggttgtaaac caaatcaaca gcagccatcc
      721 ttcacaactg aatggctata ttgaagtggg tggacgacgt gcagaagtcg ttattgccaa
      781 tccggcaggg attgcagtca atggtggtgg ttttatcaat gcttcccgtg ccactttgac
      841 gacaggccaa ccgcaatatc aagcaggaga ctttagcggc tttaagataa ggcaaggcaa
      901 tgctgtaatc gccggacacg gtttggatgc ccgtgatacc gatttcacac gtattcttgt
      961 atgccaacaa aatcaccttg atcagtacgg ccgaacaagc aggcattcgt aatcaagggc
     1021 agttgtttgc ttcttccggt aatgtggcga ttgatgcaaa tggccgtttg gtcaatagtg
     1081 gcacgatggc tgccgccaat gtgcaagata tgaataatac agcggaacac aaagtcaata
     1141 tccgcagtca agcctttgaa aacagcggta cggcggtatc gcaacaaggc actcaaattc
     1201 acagtcaatc gattcaaaac actggcaaat tattgtcggc aggaacagag gatttagccg
     1261 tttcaggcag cctgaacaat caaaatggcg aaatagcgac caatcaacaa ctgattattc
     1321 acgatggtca gcaatctacc gttgtcattg ataatacgaa tggcacgata caatcaggcc
     1381 gtgatgttgc cattcaggca aaatcgttat ccaacaacgg cacacttgcc gctgataata
     1441 aactggatat tgcgttacaa gatgattttt atgtagaacg caagatcgtg gcgggcaatg
     1501 aattgtcgct cagtacacga ggcagcctga aaaattcaca taccttgcaa gcaggaaaac
     1561 gcattcggat taaagcaaat aaccttgata atgcagtaca aggcaacatt caatccggcg
     1621 gtacgacaga cattggcacg cagcacaatt taaccaatag aggcttgatt gacggacaac
     1681 aaaccaaaat ccaagccggg caaatgaata atatcggtac aggtcggatt tatggcgaca
     1741 atatcgctat tgcggctacc cgcttagaca atcaagatga aaacggtaca ggtgccgcca
     1801 ttgcggcacg cgaaaacctg aatttaggca ttgaacaatt aaataaccgt gaaaacagtc
     1861 tgatttacag cggtaacgat atggcggttg gcggcgcatt agataccaat gaccaagcca
     1921 caggcaaagc ccaaaggata cacaatgccg gcgcaatcat tgaagctgca ggcaaaatgc
     1981 gtttaggtgt agaaaagctg cacaatacca atgaacattt gaaaacgcag ttggtagaaa
     2041 cagggcgcga gcgtattgtt gattacgaag catttggacg acacgaatta ttgcgagaag
     2101 gcacgcaaca tgaattaggc tggtttgtct acaacaatga atcagaccac ttacgcaccc
     2161 ctgatggagt ggcgcatgaa aattggcata aatacgatta tgaaaaagta acgcaagaaa
     2221 ctcaagtaac cggaactgcg cctgctaaaa tcattgcagg tagcgatttg attattgata
     2281 gcaaagcagt cttcaacagc gacagccgaa tcatcgccgg cggcaatttg cttgtgcaaa
     2341 cagaaaaaga cggtttgcat aacgagcaaa cctttggcga gaagaaagtc ttcagcgaaa
     2401 atggtaagtt gcacaactac tggcgtgcgc gtcgtaaagg acatgatgaa acagggcatc
     2461 gtgaacaaaa ttatactttg ccggaggaaa tcacacgcga catttcactg ggttcatttg
     2521 cctatgaatc gcatagcaaa gcattaagcc gtcatgcgcc cagccaaggc actgagttgc
     2581 cacaaagtaa ccgggataat atccgtactg cgaaaagcaa cggtatttcg ctaccctata
     2641 cgcccaattc ttttacccca ttacccggca gcagcttata cattatcaat cctgccaata
     2701 aaggctatct tgttgaaacc gatccacgct ttgccaacta ccgtcaatgg ttgggtagtg
     2761 actatatgct gggcagcctc aaactagacc caaacaattt acataaacgt ttgggtgatg
     2821 gttattacga gcaacgttta atcaatgaac aaatcgcaga gctgacaggg catcgtcgtt
     2881 tagacggtta tcaaaacgac gaagaacaat ttaaagcctt aatggataat ggcgcgactg
     2941 cggcacgttc gatgaatctc agcgttggca ttgcattaag tgccgagcaa gcagcgcaac
     3001 tgaccagcga tattgtttgg ttggtacaaa aagaagttaa acttcctgat ggcggcacac
     3061 aaaccgtatt gatgccacag gtttatgtac gcgttaaaaa tggcgacata gacggtaaag
     3121 gtgcattgtt gtcaggcagc aatacacaaa tcaatgtttc aggcagcctg aaaaactcag
     3181 gcacgattgc agggcgcaat gcgcttatta tcaataccga tacgctagac aatatcggtg
     3241 ggcgtattca tgcgcaaaaa tcagcggtta cggccacaca agacatcaat aatattggcg
     3301 gcattctttc tgccgaacag acattattgc tcaatgcggg taacaacatc aacaaccaaa
     3361 gcacggccaa gagcagtcaa aatgcacaag gtagcagcac ctacctagac cgaatggcag
     3421 gtatttatat cacaggcaaa gaaaaaggtg ttttagcagc gcaggcaggc aaagacatca
     3481 acatcattgc cggtcaaatc agcaatcaat cagatcaagg gcaaacccgg ctgcaggcag
     3541 gacgcgacat taacctggat acggtacaaa ccggcaaata tcaagaaatc cattttgatg
     3601 ccgataacca taccatccga ggttcaacga acgaagtcgg cagcagcatt caaacaaaag
     3661 gcgatgttac cctattgtca gggaataatc tcaatgccaa agctgccgaa gtcggcagcg
     3721 caaaaggcac acttgccgtg tatgctaaaa atgacattac tatcagctca ggcatccatg
     3781 ccggccaagt tgatgatgcg tccaaacata caggcagaag cggcggcggt aataaattag
     3841 tcattaccga taaagcccaa agtcatcacg aaactgctca aagcagcacc tttgaaggca
     3901 agcaagttgt attgcaggca ggaaacgatg ccaacatcct tggcagtaat gttatttccg
     3961 ataatggcac ccggattcaa gcaggcaatc atgttcgcat tggtacaacc caaactcaaa
     4021 gccaaagcga aacctatcat caaacccaaa aatcaggatt gatgagtgca ggtatcggct
     4081 tcactattgg cagcaagaca aacacacaag aaaaccaatc ccaaagcaac gaacatacag
     4141 gcagtaccgt aggcagcctg aaaggcgata ccaccattgt tgcaagcaaa cactacgaac
     4201 aaaccggcag caacgtttcc agccctgagg gcaacaacct tatcagcacg caaagtatgg
     4261 atattggcgc agcacaaaac caattaaaca gcaaaaccac ccaaacctac gaacaaaaag
     4321 gcttaacggt ggcattcagt tcgcccgtta ccgatttggc acaacaagcg attgccgtag
     4381 cacacaaagc agcaaacaag tcggacaaag caaaaacgac cgcgttaatg ccatggcggc
     4441 tgccaatgca ggttggcagg cctatcaaac aggcaaaggc gcacaaaact tagccaatgg
     4501 tacaaccaat gccaaacaag tcagcatctc cataacctac ggcgaacagc aaaaccgaca
     4561 aaccacccaa gttcaagcca atcaagccca agcgagtcaa attcaagcag gcggcaaaac
     4621 tacccttatt gccgaaggtg cggcgaacaa tccaatatca acatcacagg ctcaggtgtt
     4681 tcaggcagag caggaaccgg cctgattgcc gataagcaaa tccatctgca atcagccgag
     4741 caaagcaata ccgaacgcag ccaaaacaaa tcagcaggct ggaacgcagg tgctgccgta
     4801 tcattcggac aaggaggctg gtcattaggc gttgccgcag gcggcaatgt cggcaaaggc
     4861 tacggcaatg gcgatagcgt aacccaccgc catagccata ttggcgacaa aggcagccaa
     4921 acccttatcc aaagtggtgg cgataccatc atcaaaggcg cgcaagtacg cggcaaaggc
     4981 gtacaagtca atgccaaaaa cctaagcatt caaagtgtac aagatagaga aacttatcaa
     5041 agcaaacaac aaaacgccgg tgcacaagtt accgtaggtt atggcttcag tgccagtggc
     5101 gattacagcc aaagcaaaat ccgagccgac catgcttcgg taaccgagca aagcggtatt
     5161 tatgccggag aagacggcta tcaaatcaag gtcggaaacc atacaggcct caaaggcggc
     5221 atcatcacca gcagccaaag cgcaaaagac aagggtaaaa accgattcag cacaggcaca
     5281 ctcgccggca gtgatattca aaattacagc caatacgaag gaaaaagttt tggattgggt
     5341 gccagcgttg ccgtaagcgg caaaacactg ggacagggcg caaaaaataa acctcaagac
     5401 aaacacctga caagcatagc cgataaaaac ggcgcaagtt catcagtagg gtacggcagc
     5461 gacagcgaca gtcaaagcag catcacaaaa agcggcatca atacccaaaa acattcaaat
     5521 cacagacgaa gccgcacaaa tcaggctgac aggcaaaata gcggcacaaa ccaaagccga
     5581 tattgataca aacgtaacca cagacaccgc cgaacgacat tcgggcagcc tgaaaaacat
     5641 atttgacaaa gatagagtgc aaagtgaact ggatttacaa agaaccgtca gccaagattt
     5701 tagtaaaaat gttcaacaaa ccaataccga gattaaccaa catttagaca aactcaaagc
     5761 agacaaagaa gcagccgaaa cagcagcagc cgaggcatta gccaatggcg atatggaaac
     5821 tgccaaacgc aaagcccatg aagctcaaga tgcggcagca aaagcagata attggcaaca
     5881 aggcaaagtc attctcaaca tgttagcctc aggtttagct gagccgaccc aaagcggagc
     5941 gggcatcgct gcggctaccg catcgccaga cgtatcgtat gcgattggac agcactttaa
     6001 agatttagcc ggtcaaaacg cgaatggcaa actaaccgcc agtcaagaaa ccgctcacgt
     6061 tcttgcccac gcggtattag gagcagcggt tgccgcagca tgaggcaaca atgccccggc
     6121 aggagcattg ggtgcgggcg ggtcggaagc ggccgcccca atcatcggca aatggctgta
     6181 cggcaaagga gacggcggca gcctgaatgc ggaggaaaaa gagaccgttt cggcgattac
     6241 aaggatgctg ggtacggctg ccggagcagc tgagggaaac tcgtccgccg atgctgtgtg
     6301 gggttgtttt cagacggctt cagatttcgc ttcctctttt tcatatccta taaacatgtg
     6361 attaaatttt catttatgga gtttttttat ctttttgttt gtttttgtgc attgctataa
     6421 ttgcccgctt acattactta gaccaatcct atgaaacctt tcttttcctc caaaaatgta
     6481 tttcatccct atcccttatc actgttagtc ctatcattga tggctgcctc cccaccctaa
     6541 catcagccgc ccccacacct gaagctgttt cacaacaaca ggatattctc caacgtcaac
     6601 gtgagaagca attgcgtgaa cagatgcagc cggaacaaga tgttcgtttg gatggtaccg
     6661 atacgggcat agagaagatg gcaacacagg taggcggtgc aaattcggat gaggcaagcc
     6721 cctgctttcc tatttctgag gtggaattgg tgggtgaaga agcggctaaa ttccggtttg
     6781 cgctcaacca tgccttgtgc caaacacatt ttgtttccgg caagtgtctg catgcgggcg
     6841 acattaatca aatcatgtcc ttagcacaaa atgctttgat cggcagggga tataccacga
     6901 cccgtatctt ggctgcgcca caggatttga atagtggcaa gcttcaatta accctgatgc
     6961 cgggctatct gcgctccata cgaatcgatc ggtccaacga tgatcaaacc catgcaggac
     7021 gtattgcagc attccaaaac aaatttccca cccgctcgaa cgatctgttg aatctgcgtg
     7081 atttggaaca aggactggaa aatctcaaat gtctcccgac tgcggaagcc gatctccaaa
     7141 tcgttcccgt agagagagaa ccaaaccaaa gtgatgtcgt ggtgcaatgg cggtaacgtc
     7201 tgctgcccta ctgtgtgagt gtggggatgg ataattcggg tagtgaggcg acaggaaaat
     7261 accaaggaaa tatcactttc tctgccgaca atccttttgg actgagtgat atgttctatg
     7321 taaattatgg acgttcaatt ggcggtacgc ccgatgagga aaattttgac ggccatcgca
     7381 aagaaggcgg atcaaacaat tacgccgtac attattcagc ccctttcggt aaatggacat
     7441 gggcattcaa tcacaatggc taccgttacc atcaggcggt tttcggatta tcggaagtct
     7501 atgactataa tggaaaaagt tacaacactg atttcggctt caaccgcctg ttgtatcgtg
     7561 atgccaaacg caaaacctat ctcagtgtaa aactgtggac gagggaaaca aaaagttaca
     7621 ttgatgatgc cgaactgact gtacaacggc gtaaaaccac aggttggttg gcagaacttt
     7681 cccacaaagg atatatcggt cgcagtacgg cagattttaa gttgaaatat aaacacggca
     7741 ccggcatgaa agatgctctg cgcgcgcctg aagaagcctt tggcgaaggc acgtcacgta
     7801 tgaaaatttg gacggcatcg gctgatgtaa atactccttt tcaaatcggt aaacagctat
     7861 ttgcctatga cacatccgtt catgcacaat ggaacaaaac cccgctaaca tcgcaagaca
     7921 aactggctat cggcggacac cacaccgtac gtggcttcga cggtgaaatg agtttgcctg
     7981 ccgagcgggg atggtattgg cgcaacgatt tgagctggca atttaaacca ggccatcagc
     8041 tttatcttgg ggctgatgta ggacatgttt caggacaatc cgccaaatgg ttatcgggcc
     8101 aaactctagc cggcacagca attgggatac gcgggcagat aaagcttggc ggcaacctgc
     8161 attacgatat atttaccggc cgtgcattga aaaagcccga atattttcag acgaagaaat
     8221 gggtaacggg gtttcaggtg ggttattcgt tttgatgcgg ttgaagtgaa taatgtatga
     8281 caagatgcac aaaagctgcc ggttttttgc aggcagcttt tgttttatgt ttggggctgt
     8341 accggacaac cgggtcaaat ttccattagc tcattatttt aaaattgaaa ttttatattt
     8401 tatttcactg ttgttaaaac gctgttcaca tttcttcatc ggttttatca ttcatcacct
     8461 tatttacggc ttctggagac aggggcttac cgcatttgct gcagtaatcg atttttggga
     8521 caggggaaat gcctttaaag aaagtaaagc tatccgaccc gatgccgacg gaggtgccat
     8581 aattcatctt ttggaattcg tggcaatagg ggcagattcc tgcgatgatt atgtagaata
     8641 aaattatgat acctccgaaa ctgaaatata tcacaaatat tatttgcata attgttattt
     8701 gcagaaaatt agaacccgtt atcacgtctt ggataaatgc caaaaccact gtgattccga
     8761 gaattaaaaa gaaaaatttc ggaatcctca tccttttctg aaacctgatt gcggtatcca
     8821 tggctttgtc tttgtcattt aagtttgtgt tcatgttttt gaccctcttt gtttctaaaa
     8881 tcctttttaa attgccgact ttttccgttc caaatgtccc atttgaacat tatcccgctg
     8941 cctaaaatac gacccaaatt catcggttgt tcttgctgcg atacctgcta acaacagttc
     9001 aacgggttta ttttgtttat tctaacctat taccgtttaa ataaatttcc aaaggcttgg
     9061 gcaggccgta atctttcaaa tggcccggct ttatccaaat gccgtctgaa tggtgttcgg
     9121 acggcatttg cccttcaaac ggcgtaatca tcagcagccg gtgcgtcagg cggtgggtca
     9181 gggcggtttg ttcgtccata tccgccatcg tcagggagag tttggcggca aagtcggaaa
     9241 gcccgttcaa actttcaaaa cacggcacgc aatacagtcc gccccaaatg cctttggcgg
     9301 tgcgtttttc cagcaagatt gcgccgtccc ggttgcggac aatcagccag taaagcggca
     9361 gggtttgcac ttcgagggca gtttttttac gcggcagctc ggcggtgcgg ttttgctttt
     9421 ttgcttcgca gatgtccgcc atagggcatt gacggcacaa gggtttcgtc cgtttgcaca
     9481 cggtcgcgcc caaatccatc aaaccttgcg tgtaagtagg catatcggcg ttttcagacg
     9541 gcatcaggct ttcggcaagt gtccagagcg agttttcaaa ttttttatct tgcggattgc
     9601 cgtcttgggc aaaaacacgg cagagaacgc gtttgacgtt gccgtccaag atggtttctc
     9661 gtcggttgaa agcaaaggca gaaatggcgg cggcggtgct tctgcctacg ccgcagagcg
     9721 tttccaagtc tttgcgctcc gatggaaacg taccgccgaa ttgtccgacg atttgttgcg
     9781 cggctttgtg cagattgcgc gcgcggccgt aatagcccaa gcccgcccac aacgacaaca
     9841 cttcgtcttg cggcgcggcg gcaagcgtct gaacggtcgg gaatttttcc aagaaacgcg
     9901 gatagtagtc caacacggcg gcgacctgcg tttgctggag catgatttcg gaaagccaga
     9961 cgcaataagg gtttttgacc tgccaaggga ggtggtggcg accgtgttgt ttttgccagc
    10021 ggatgagccg ttcggagaag gggatgggtg tgttcattaa tatcaatcgg tggttttatt
    10081 tatatttaaa acagtatgtt attgtataaa attgtgaaaa taattcttat tgacttattt
    10141 tttgtagggg catataactc atataaagaa actttattgt ggtattgaaa ttatttatca
    10201 acaagcaagg agtatcagca tgaaagcaat ggtttatcac ggcgcaaacg acatccgttt
    10261 tgaagaaaaa ccccgcccgc agattatcga tccgaccgat gcggtggtga aaatcgtcaa
    10321 aaccacgatt tgcggtaccg acttgggtat ttggaaaggc aaaaaccccg aagtcgccga
    10381 cggtcgtatc ctcggtcatg aaggcatcgg tattgtagaa gaagtcggcg aggctgtaaa
    10441 aaacatcaaa gtcggcgata aagtcattat ttcatgcgtc agcaaatgct gcacttgcga
    10501 caactgcaaa atccaacttt attcgcactg ccgcaacggc ggctggattt tgggctatat
    10561 gattgacggc acgcaggccg aatacgtccg cacgccttat gccgacaaca gcctcgtgcc
    10621 gccgcacgac aacgtcaacg aagaaatcgc cctgctgttg agcgacgctt taccgaccgc
    10681 ccacgaaatc ggcgtgcaat acggcgatgt gaaacccggc gataccgtat tcatcgcagg
    10741 tgccggccct gtcggtatgt ccgccttgtt gaccgcccaa ctgtatagcc ctgccgccat
    10801 tatcgtgtgc gatatggacg aaaaccgttt gaaaccggcg aaagagttgg gcgcgaccca
    10861 taccatcagc cccgcttccg gcgacgtctc caaacaagtt ttcgccatcg tcggcgaaga
    10921 cggcgtagat tgtgccatcg aagcggtcgg tatccctgca acttggaata tgtgccaaga
    10981 catcgtgaaa cccggcggtc atatcgccgt cgtcggcgta cacggtcaat ccgttgattt
    11041 caaactggaa aaactgtgga ttaaaaatct tgccatcact accggtttgg tcaatgccaa
    11101 caccaccgaa atactgatga aggcaatttt cagcagctca gtggattaca ccaaaatgtt
    11161 gacccaccgt ttcaaattca gcgaattgga aaaagcctac gacgtgttca aacacgccgc
    11221 cgaaaaccaa gcgatgaaag tggttttgga agcggattaa tcgccgttga atcagttgtt
    11281 ttttaagcgg atgccgtctg aacgtgtttc agacggcatt ttttcagccg tgtaaaacgg
    11341 gctgttcctg atcaatcagg tatagtggat taaatttaaa tcaggacaag gcaacgaagc
    11401 cgcagacagt aaaatagtac ggcaaggcga ggtaacgccg tactggttta aatttaatcc
    11461 actatattta agggggatta atgggatatt ttggtgggaa cggcggttat ttgaaacgaa
    11521 aacagccgaa aaacctgtgg ttggatgtcg gctgtttgaa gaaaaggaat tttgcaaagg
    11581 tctctgagcg ttatttttta acgacggtta acttgttagt ttcagccgct catcacggca
    11641 ttttaccgat agggcgattt ttatgactaa ttgataaata tattataatt attttttcgt
    11701 cataggtctc cgcatatcgc gcgtcattcg cattgttctg gagatggatg ggcattttag
    11761 gggcggtgct gctgtgtatg ccgactgagt cgtcaagcgg caaaatgccg tctgagactg
    11821 gaaagggctt cagacggcat ttttacgatg cggttcaggc tttgggtttc aatgccgccg
    11881 cttctttggc gaggcgggta atggtgtccc agtctttgtt ttttacagct tctttcggtg
    11941 tcagccaaga gccgccgacg cataagacgt tgggcaatgc caagtaatcg ggcgcagtgg
    12001 cgaggctgat gccgccggtc gggcagaagc gtacgtcggc gtaaggtccg tagagggctt
    12061 tgagcatcgc tttgccgcca acgacttcgg cagggaagag tttgagtgtg tcgatgccgt
    12121 gttccaaagc cagttggact tcgcccggag tggcaacgcc gggaatcagg ggaatgccgc
    12181 tgttgcggcc ggctctggcg agggattcgt gcaaacccgg gctgatggcg aaaaccgcgc
    12241 ctgcgtcttc gacggctttg agctgttcgg gattggttac cgtacctgcg ccgatgatgg
    12301 cgttgggcat ttctttggca atcaggcgga tggcctcgag tccgacgggg gtgcgcaagg
    12361 taatttcgag ggtggggatg ccgccttcga caagggcgcg ggacaaatcg acggcggtgc
    12421 ttaagtcgtc aatcgccatc acaggcacaa ctgcgccggc ggtcaggatt tggcgggggg
    12481 tcagtttgga catttcggtt ctccaagtgg ggggtgtcgt ttttgttgag gtcaaacggg
    12541 atgggggggt taagttttca gacaaccttt ggggtaaaac attctgatgt tgcttgggtt
    12601 ttgttcgcgg gcaaagctca cgctacgtct gcatctattt taaacggctt caataatttt
    12661 tcagacggcc ttctgtttta aaaggccgtc tgaaacgcgc atcaggcaaa ttctccgccg
    12721 aagctcatcg caccggtttc cgcactgctg gtcatactgc ggaagccggc gaagagttcg
    12781 cggccgcagc cttgttggtt cgcgcccaaa tcgatgcgct cgacttctcg gacgttccat
    12841 tcggcctcgt taatcaggac gttgagttcg ccggtaacgg agtcgaagcg aatcaggtcg
    12901 ccggtacgga ttttggcgat gttgccgccc atcagggctt cgggcgtcat gtggatggac
    12961 gcgggcactt tgcctgacgc gccggacata cggccgtcgg tcagcagcgc cactttgaag
    13021 ccgcggtctt gcaggatgcc caaaggcggg gtcagtttgt gcaattcggg cataccgttg
    13081 gcacgcgggc cttggtagcg gacgacgcag ataaaatcgc gttccaactc gccgcgttca
    13141 aatgccgcca acacttcgcg ctggtcgttg aacacgatgg cgggtgcttc gatgatgcgg
    13201 cagccttcgc gtacggctga cactttaacc acgccgcgtc cgatgttgcc tttcatcagg
    13261 cgcagaccgc cgtccgggga gaacggattg tcggctttac gcaggatgtc gtcgttgccg
    13321 ctggtctcgg gggcttcgcg ccattcgagt ttgccgtcga tgaggaaagg ctctttagtg
    13381 tagtggcgca taccgtgtcc gacgacggta tcgacatcgt cgtgcaataa gcctgcgtcc
    13441 agcaattcgc ggataacgaa aggcaggccg cctgctgcgg taaagtggtt cacgtcggct
    13501 ttgccgttgg gatacacgcg gatgagcagc gggatgatgg aagaaatttc gtcgaagtcg
    13561 tcccagttca aaatcacgcc tgcggcgcgc gccatagcga cgaggtgcat ggtgtggttg
    13621 gtcgagccgc cggtcgccat caaaccgatc agggcattga tgaaggattt ttcggtcaac
    13681 atttcgccca aaggtttaat cgtgccgttt ttgatgccgc gcgcgaggtg tccggcggcg
    13741 tagcgggtca gggcttcgcg caggtcggta taggggtgga caaaggcggc ggcaggcagg
    13801 tgtacgccca tcatttccat catcatttgg ttggagtttg ccgtgccgta gaaagtgcag
    13861 gtgcccgggc tgtggtaaga acccatttcg cttttcaaaa gttcgtcgcg tccgactttg
    13921 ccttcggcga aaagttggcg ggtacgggct ttttctttat tgccgatgcc gctggacatc
    13981 gggcctgccg gaacgaagat accgggaata tgaccgaacg aaagcgcacc tatcatcaga
    14041 cccggaacga ttttgtcgca cacgcccata aacaggctgc cgtcaaacat ttggtgcgac
    14101 agcccgacgg cggtactcat cgcaatcacg tcgcgggaaa acagtgacaa ttccatgccg
    14161 gcgtagcctt gcgtgatgcc gtcgcacatg gcaggcgtgc cgccggcgac ttgtgcggtc
    14221 gcgccgtttt tttgtgcttc gtctttgatt tggtcaggga agtctttaaa cggctggtgt
    14281 gcggaaacca tgtcgttgta ggcagtgatg atgcctaagt tggggacggt ttcctgaagc
    14341 atttcgattt tgatgctttt gggcatggcg gcatagccgt gcgccaaatt gctgcagccg
    14401 agctggttgc gctctaagcg tcccatctgt ttggcactgc ggattttcgc cagatatttt
    14461 tcacgcgtcg gacggctgcg ttcgataatg cgcccggtga tttcggcgag tttggggtgg
    14521 ataggagtgt ggttcatgtt cggtctcctg gcggaacggg gtggtattgg ggatttgttt
    14581 gtgcgtttct gatttttgta tcgggtatgt tagacggtga gtatagtgga ttaaatttaa
    14641 accggtccgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc tcgccgcctt
    14701 gtcctgattt aaatttaatt cactatattt taaaaggtac gccgtctgaa agtgggaagc
    14761 gggtgctatt atagtacaaa atttgtaatt ttgttacaaa aaatcagaca ttgaaaagaa
    14821 atttgattta aatcgcagaa ttttctgatt gttgtaacgg aataggggaa acctgcctga
    14881 tttttggact aggtcaaata aattgtaaaa atgggaatag acaaaaagtg tagcggtgtg
    14941 tagtattact actcattatg ccgcctgcct ttgtttccga tattgcgatg ctgcgtgatg
    15001 gaaaaaacag gtttcagacg gcatcgaaaa aatcctttcc tgacgagata atgcgatgag
    15061 tacacagaca aattttgatt tggtgttgtt cggtgcgacc ggcgatttgg caatgcgcaa
    15121 acttttgccc tgcctgtatc aggcgcatgt tgccggtctg cttcatcccg aagggcgtat
    15181 tttgggggtc agccgcagcg agttggatac cgaaggtttt ctggcgaagg tggaaaccag
    15241 ttccaaaatc catgtgaaag aaaatttttc cggtgaggcg tgggcttcgt ttgttgaacg
    15301 tctcgcatat ctcaaagtcg atgttacgca gccggacgat tttgccgctt tgggcgattt
    15361 ggtcaaagca cgcaaagaaa ccgacaacgt cgtcatctat ctgtccaccg cgccgaaatt
    15421 cttcgcgcaa gcctgcgaaa accttgctgc gatcggtttg aatgccgaca atgtgcgcgt
    15481 ggttttggaa aaaccgctgg gtacggattt ggcttcttcc caacaaatca ataccgatgt
    15541 ggcgcgttat ttcaaagaag ggcaaattta ccgcatcgac cactaccttg gcaaagaaag
    15601 cctgcaaaac ctgcttgcgc tgcgttttgc caatgtaatg ttcgagccgc tgtggaacaa
    15661 taaatacatc gaaagcgtgc agctgaccat cgccgaacag ctcggtgtgg aagagcgcgg
    15721 cgagttttac gacattaccg gcgcgttgcg cgatatggtg caaaaccatt tgatgcagat
    15781 gctgtgcatg actgcgatgg aagcccccgc cagcttggat gccgacgcgg tgcgcgatga
    15841 aaaagtcaaa gtcatcaagt cattgaagcc gctgaccatc gaatctgtca atgaaaatgt
    15901 cgtgcgcgga caatataccg ctgccaaagg catgaacggc tatcttgaag aaatcaacgt
    15961 tccgcaagac agctttaccg aaacctacgt cgccattaaa gccgaaatcg aaaacgaacg
    16021 ctggaagggc gttcccttct acctgcgtac cggcaaacgc atggcgggca aagtggcgga
    16081 aatcgttttg aacttccgcc cgctgcaaaa ccatattttc gacaacagcc aaaccgcgcc
    16141 gaaccgtttg gttatcgagc ttcaaccgaa tgaatccgtc cgcctttaca cgcaggtgaa
    16201 aaccccgggt gcgggcaaca aagtggaagt aacgcctttg ggcgtggatt tgggcaaggc
    16261 cgttgaaggc cgccgcgccg aggcttacga acgcctgctg ttggatgtga ttaacggcaa
    16321 actcgctttg ttcaaccgcc gcgacgaact cgaagccgcg tgggaatatg tgatgccgat
    16381 tttggaaaac tgggcaaata acaccacgcc gccgcacggt tacggcgcac actcttgggg
    16441 ccctgaagcc gcccgcgaac tgttggcgcg cgacggcaac aagtggcacg aagaacagta
    16501 atgcgcttca gacggcatcg ggttcgcaat gccgtctgaa ggagggcggg cagaaaaagc
    16561 agtcccgctt cgaggcggaa gcggctaagc cattgcccgg cagccgccct gtaacgggat
    16621 atgccgtctg aaatcacaaa aggacacaaa tatgtttgtt tggcacgaat acgaaaatgc
    16681 ggcagaagcg gcgcagtctt tggctgacgc agtggcggat gctttgcagg gcgcactgga
    16741 cgagaagggc ggtgcggtgt tggcagtttc cggcggacgt tcgccgattg catttttcaa
    16801 cgccctgtcg caaaaagatt tggattggaa aaacgtcgga attaccttgg cagatgaacg
    16861 catcgtgccg accaaccacg ccgacagcaa taccggtctg gtgcgcgaat acctgttaaa
    16921 gaacaaagcc gctgcggcag tgtggattcc tatggtggaa gacggaaaaa ctgaaaccga
    16981 attacatccc gatgctgttg tcgattatgc gctgaaacat tacaagcagc ccgatgtttt
    17041 gattttgggt atgggaaacg acgggcatac ggcttcgatt ttcccaaaag ctccgcagtt
    17101 tcagacggca atcgacggtt cggcaggtgt cgcgctggtg catactacgc ccgttaccgc
    17161 gccgcacgag cgcatcagca tgactttgga tgcgattgcc catactgggc atgtattttt
    17221 ggcgatacag ggcgaagaga aaaaagccgt gttcgaccaa gccgcacaag gcgaaaaccg
    17281 cgaatatccg atcagcctcg ttttgaacca tcaaggagtg aactgccatg tcttctacgc
    17341 cgaataaaca cgccgattac ccgaggctgg ttgccgacat cggcggcaca aacgcccgtt
    17401 tcgcattgga aaccgcgccg tgcgtgattg agaaagtcgc cgtcctgccg tgtaaggaat
    17461 acgacacggt taccgacgcg gtgcgtgcct atctgaacca aagtggtgca acgggcgtac
    17521 ggcacgcggc atttgccatc gccaacccga ttttgggcga ctgggtgcag atgaccaacc
    17581 accattgggc gttttccatc gaaaccaccc gtcaggcttt ggggctggac accctcatcc
    17641 ttttgaacga ctttaccgcg caggcattgg cggtaacgca gacttcaagc aaagacctga
    17701 tgcaggtagg cgggcaaaag cctgtcgaat tcgcccccaa ggccgttatc ggccccggta
    17761 ccggcttggg cgtgagcgga ttggtgcaca gccccgcagg ctgggtggcg ttggcgggcg
    17821 agggcgggca taccagtttc ccgccgtttg acgatatgga agtgttgatt tggcagtacg
    17881 ccaaaaacaa ataccgccac gtttccgccg aacgtttcct cagcggcgca ggtttgagcc
    17941 tgatttatga aacgctggcg gccaagcaga aagccgaacc ggcaaaactg atgccgtctg
    18001 aaatcaccga aaaggcatta aattgcgaat cgcccctgtg ccggcaggca ttggacattt
    18061 tttgcgcgat gctgggcacg gtcgcttcca acctcgccct gactttgggc gcgcgcgggg
    18121 gcgtgtattt gtgcggcggc attatcccgc gaatgttgga ttacttcaaa acctccccct
    18181 tccgcagccg ctttgaaaac aaagggcgtt ttgaagccta ccttgccgcg attcccgttt
    18241 atgtcgtctt gagcgaattt ccgggtattg ccggcgcggc ggcggcgctc ggcaaccacc
    18301 tgaaaaacgt ctaaccacgg cagcccttgc agcagggccg cattatcgaa gggcatatca
    18361 ttatgttaag caaaatcagc gaatcactgg ccgacctgtc cggtgcggaa cgcaaagttg
    18421 ccgaatgcgc cttggcagaa cccaagtggt ttgtccacgc cgccgtcgcc gaaatcgccg
    18481 accgcgcctc ggtcagccag ccgaccgtca tccggttctg ccgcagcctc ggttataagg
    18541 ggctgccgga attcaagctc tccctgtccg ccagcatcgg gcacgaaggt atgccttatg
    18601 tccacgaaga actcaatgcc gacgacgata tggcgaatgt ggttgaaaaa gtgttgggca
    18661 acgccgccgc agccttgttg ggggagagga ggttcttaaa agaatccgaa ctcgaaaacg
    18721 gcatcgccac cctgatgcac gcccgccgcg tcgaatttta cggcgtgggc aattccggca
    18781 ttgtggcaca ggacgcgcag cacaaatttt tccgtttcgg catgtccacc gtcgcctatg
    18841 tcgatacgca cacgcaactg atggcggcat ccgttttgag cgatcaggat gttttggttg
    18901 ccatttccaa cacgggttcg tctatcgaac ttttggatgc ggtcagcatc gccaaagaaa
    18961 acggcgcgtc cgtcatcgca ctgacccgca acgattcgcc tctggcgcaa cttgccgact
    19021 gcgtgttgag cgttgccaca caggaaaacg ccgaactcta cacgcccatg gtttcccgcc
    19081 tcttgcagct tgccgtcatt gatattctcg ccatcggact tgccctgcgc ttgggcgatg
    19141 ctgccagcct gcaattgcag aaaagcaaaa aaagcataca caacaagcac atcgattacg
    19201 acaaagactg atgttcagac ggcatcccgc caatgccgtc tgaacggccg gacaggctcc
    19261 ggcaagcctt ttatcagaat ttccttaatg gcggaaaccc cgccctttaa ggcggcaggg
    19321 ttcgggtttg ccgcaacccc ttccaaatcg gggcggtacg cagggttgcg ccttatgtgc
    19381 gcatcgatgt gcggaaacgc cccccgtcac ggaaaacctc aatcgggagc atacagatga
    19441 aacaccttca cgacttaccg gcctggtcaa aattgtggaa ccactttgac gacagcaaaa
    19501 cattgcatat gcgcgaaatg ttcgagcaag acccgcagcg ggcggaacgc tactggctgc
    19561 aagtcggcgg actgacgctg gactactcca aaaaccgcat caacgacgaa accatgtccc
    19621 tactgttcga gttggcgcgt gaagcaggcg tgccggagcg gatgcggcag atgttccacg
    19681 gcgaaaaaat caataccacc gaaaaccgcg ccgtcctgca tgtcgccctt cgcaaccgca
    19741 ccaattcgcc gattatggtt gacggtgaag atgtgatgcc caaagtcaac cgcgttttgc
    19801 aacgtatggg cgaatttgca cacgaagtcc gcagcggaag ctggctgggc tataccaacc
    19861 aagtcattac cgacgttgtc aacatcggca tcggcggatc ggatttgggc ccgctgacga
    19921 tgtgtaccgc gctcaaacct ttcggtcatc cgcgcctcaa tatgcacttc gtctccaacg
    19981 tggacggctc gcaactgcgc gacgtattgt ccaaagtcca cccggaaacc acgctgttca
    20041 tcatcgcctc caaaacattt accacgcagg aaacgctgac caacgcgcta accgcgcgcg
    20101 aatggttttt gaatcatgcg ggtgacgaag aagccgttgc caaacacttc gccgccgttt
    20161 ccaccaatcg aaaagccgtc gccgaattcg gcatcgacat cgccaatatg tttgaatttt
    20221 gggattgggt cggcggtcgg tacagcctgt ggtccgccat cggattgccg attatgctgt
    20281 atctcggcga agaaaacttc atcgaaatgc tcaacggcgc gcacctgatg gaccaacact
    20341 tcatcaacac cccgctcgag cgcaacctgc ccgtcatcct cgccctcatc ggcatctggt
    20401 atatcaacta ctacggtggc ggcagccacg tcatcgcgcc ttacgaccaa catttgcacc
    20461 gcctgcccaa attcatccag cagctcgata tggaaagcaa cggcaaacag gttacgttgg
    20521 acggcaaagc agtcggacac gaaacctcgc cgattatctg gggagaaacg ggcattaacg
    20581 gtcagcacgc cttcttccaa ctgctgcatc aaggcacgca catcaccccc atcgacctga
    20641 ttgcctcgct tgaaaaacgc agcaacctgc ccggacacca cgaaatcctg cttgccaacg
    20701 tctttgcaca agcagaagcc tttatgtgcg gcaaaacccc cgacgaagtc cgcgccgaac
    20761 tcaaagcgca gggtatggat gaggcgcgca tcgaagagct ggttccgcac aaaaccttct
    20821 ccggcaaccg cccgaccaac ctcatcctga tggataaggt caacccccgc aatatgggca
    20881 gcctgattgc catgtacgaa cacaaaacct tcgtccaagg catcatttgg ggcatcaaca
    20941 gcttcgacca gtggggcgtg gaactcggca aacaactggc taaaaccatt ttgggcgaac
    21001 tgacaggcga aaccgaacct caaaaacacg acagctcgac cgaacgcctg attaacctct
    21061 acctgcagac caaccgcaaa taaaacctgc ggaaaaatgc cgtctgaacg ccgaccgttt
    21121 cagacggcat ttttatcgaa caggaaaacc gtcggtaaat ttgcagggcg tgtgcaatcc
    21181 cgatatggcg gtttgcataa atttaaacat atatgttccg cagccatgta cttcaagtgc
    21241 cgtagcgtgt gtggaaatac gcaacagaca gtccgtgttt ggacggcaaa ggaaatctaa
    21301 tgaaaaaaaa tttacccgca ctggcattgg caagtatgct gattttgtcg ggctgcgacc
    21361 gtttgggaat aggcaacccg ttttccggaa aggaaatttc ctgcggaagc gaagagacta
    21421 aagagatttt ggtcaaactg gtccgcgaca atgtcgaagg tgaaaccgtc aaaacttttg
    21481 acgacgacgc attcaaagac caagcatttg ccgatatcgg catatcgcat atccgcagaa
    21541 tggtcgaacg tttgggcata accgtcgatg aagtccgaac taccgagaaa accgacacgt
    21601 ccagcaaact caaatgtgaa gccgcgttaa aactggacgt gcccgacgat gttgtcgatt
    21661 atgccgtcgc cgccaaccaa tctataggca acagctataa gaaaacgccc gacttttttg
    21721 aaccctacta ccgcaaagaa ggcgcgtatt atgtcaaaac tatttcttac agcgtccagc
    21781 cgacagacga caaaagcaaa atctttgccg aactcagtca ggcacacgat atcatccatc
    21841 cgctcagcga gctggtgtct atggcactga ttaaagagcc gttggacaaa gcgaaacaaa
    21901 ggaacgaaaa acttgaagcg gcagaagcca ccgcgcagga agcgagggag gcagaagaag
    21961 cggcggcgca ggaggcattg ggtcgggagc aggaagccgc ccgcgtatcc gaatgggaag
    22021 aacgctacaa gctgtcgcgc agcgagttcg agcagttttg gaaaggattg cctcaaactg
    22081 tacagaataa gctgcaagcc tcgcagaaaa catggaaaag cggtatggac aagatctgtg
    22141 ccaacaatgc gaaagccgaa ggtgaaacgc caaacggcat aaaagtcagt gagttggcgt
    22201 gtaaaacggc agaaaccgaa gcacgcttgg aagagctgca caaccgtaaa aaagccctta
    22261 tcgacgaaat ggtcagggaa gaggacaaga aagaactgcc aaagcggctc tgaacagcgc
    22321 ggttcaggca ctgcccgccg atattgccga aaccgttatg ccccagtacc gcaactggca
    22381 aagcggtttg gatgccaaat gcgccgacgg cgacgaatac ggggtggcac aatctgactg
    22441 ccgtaccaga gaaatcaatg cgaaaaccaa agaaatccaa ggttatctga ttgactgaaa
    22501 cttggatgcg ggaatgtcgg cggcttttgc gtttttgtcg tttttaaatc cccttcgttg
    22561 cgtttggact ttgccgatac cgcatatccg agaaaggaag taatgattta ctatcccgcc
    22621 gtgctgttcc aagaccccga tcaaagcaac tggggcgtga ttattcccga tctgccgggc
    22681 tgctatcctg tcggcgatac ggttgccgac gcgcttgccg atgccaaagc cgccgcaatg
    22741 ttccatctcg aagggctttt gcaggaaaac ctgccgattc ccgaggcgca aagcatagag
    22801 gttaatcggg acaatcccga ttatgccgat gcggtgttgt ggacgatggt tgaagcggac
    22861 gatgccgcat taaccggaca ggtgcggttt aacgtcagtt ggccgcaaca tatcctcaac
    22921 cgtgtcgatg cctgcactgc cgcccgtcat gaaacccgag cggttttttg gcgaaagccg
    22981 ccttaaaggc gatgcagcct taactttcag cccgattttt tcgggctttt ttattgccga
    23041 tgccgtctga atgcggcgcg gcggcaattt taccgcgtat ccctttcatt ggcggcgcgg
    23101 ctttggttga accgcgcgtc gtgctgcctg cctttcagtt agctgatata cgcctgccgg
    23161 cagtggtggc ggtcgaaaaa tacgccgttg atcagggtat atatagtgga ttaacaaaaa
    23221 ccggt
//
LOCUS       DAXDZG010000029        22845 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXDZG010000029 DAXDZG010000000
VERSION     DAXDZG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22845
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.029"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(41..211)
                     /locus_tag="ACFB78_000986"
     CDS             complement(41..211)
                     /locus_tag="ACFB78_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC1269000.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1426)
                     /locus_tag="ACFB78_000987"
     CDS             complement(344..1426)
                     /locus_tag="ACFB78_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC1269001.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRVVKEKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVEGGYTVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1562..2110)
                     /locus_tag="ACFB78_000988"
     CDS             complement(1562..2110)
                     /locus_tag="ACFB78_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC1269002.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVLKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2250..2621)
                     /locus_tag="ACFB78_000989"
     CDS             complement(2250..2621)
                     /locus_tag="ACFB78_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC1269003.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2806..4497)
                     /gene="dld"
                     /locus_tag="ACFB78_000990"
     CDS             complement(2806..4497)
                     /gene="dld"
                     /locus_tag="ACFB78_000990"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC1269004.1"
                     /translation="MNASQLISSLTQITGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4594..5013)
                     /locus_tag="ACFB78_000991"
     CDS             complement(4594..5013)
                     /locus_tag="ACFB78_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269005.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5236..5332)
                     /gene="ffs"
                     /locus_tag="ACFB78_000992"
     ncRNA           complement(5236..5332)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFB78_000992"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5593..9426
                     /locus_tag="ACFB78_000993"
     CDS             5593..9426
                     /locus_tag="ACFB78_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC1269006.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKGVELFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9503..10504
                     /locus_tag="ACFB78_000994"
     CDS             9503..10504
                     /locus_tag="ACFB78_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC1269007.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10565..10747)
                     /locus_tag="ACFB78_000995"
     CDS             complement(10565..10747)
                     /locus_tag="ACFB78_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269008.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10920..11051)
                     /locus_tag="ACFB78_000996"
     CDS             complement(10920..11051)
                     /locus_tag="ACFB78_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269009.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(11310..12453)
                     /locus_tag="ACFB78_000997"
                     /pseudo
     CDS             complement(11310..12453)
                     /locus_tag="ACFB78_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12778..13254)
                     /gene="greA"
                     /locus_tag="ACFB78_000998"
     CDS             complement(12778..13254)
                     /gene="greA"
                     /locus_tag="ACFB78_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC1269010.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYGIIEVRYI"
     gene            complement(13518..13646)
                     /locus_tag="ACFB78_000999"
     CDS             complement(13518..13646)
                     /locus_tag="ACFB78_000999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269011.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13645..14946
                     /gene="aroA"
                     /locus_tag="ACFB78_001000"
     CDS             13645..14946
                     /gene="aroA"
                     /locus_tag="ACFB78_001000"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC1269012.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(15024..15557)
                     /locus_tag="ACFB78_001001"
     CDS             complement(15024..15557)
                     /locus_tag="ACFB78_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC1269013.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15614..17140)
                     /locus_tag="ACFB78_001002"
     CDS             complement(15614..17140)
                     /locus_tag="ACFB78_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC1269014.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(17252..18793)
                     /locus_tag="ACFB78_001003"
     CDS             complement(17252..18793)
                     /locus_tag="ACFB78_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC1269015.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFVLGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            18941..19720
                     /locus_tag="ACFB78_001004"
     CDS             18941..19720
                     /locus_tag="ACFB78_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC1269016.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19717..20418
                     /locus_tag="ACFB78_001005"
     CDS             19717..20418
                     /locus_tag="ACFB78_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC1269017.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20415..21869
                     /locus_tag="ACFB78_001006"
     CDS             20415..21869
                     /locus_tag="ACFB78_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC1269018.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIVITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(22018..22437)
                     /gene="fitB"
                     /locus_tag="ACFB78_001007"
     CDS             complement(22018..22437)
                     /gene="fitB"
                     /locus_tag="ACFB78_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC1269019.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22437..22673)
                     /gene="fitA"
                     /locus_tag="ACFB78_001008"
     CDS             complement(22437..22673)
                     /gene="fitA"
                     /locus_tag="ACFB78_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC1269020.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         5279 a         6109 c         6210 g         5247 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtctatc acgcccgcgc
      721 cgatgccgat ttgcaggagg ataaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg ggataaatac gtctttgaag ttcaggctga
      841 aggtgcgcgt gccttcgagg gcgcaaaatt cggggcagtt ttgcaggctt acgccttccc
      901 tttgcccgcc tgtgatgaac atgacgtagc cgccgccgat ttgggcggta ttcgcccaaa
      961 tgtggtcttc gccgatgttg gacacccacg gcagcgcacc gttgactgta taaccgccct
     1021 ccacgcactc ggcttgaagg ttgtgttttt caatgccggc aaggtgtttg acggtattgg
     1081 acattccggt gcccgccaat actttgcctt gcaggatgtc ggcgaggtat ttttctttga
     1141 cgacccggtt gggcgtttgg tgcagatacc acgcgcaagc cgcctgacac cacgcgctga
     1201 aggaggtcgc gccgcattct ttgccgattt cgcgcaagac ggcgattcgt gttgccaagc
     1261 ctaagccgtt gccgccttcg gcttctatac cgactgcgcc gattgcgccg agttcgcgca
     1321 taaatgcttc ggggtagtat cctttgcggt cgatgtcgtc gacgaggggt ttgagcttgg
     1381 ttttgacgag ttcggcaacg ttggcaagca gggtttgggc gttcatcttt gttccttaag
     1441 gtttacgggg gaatcggggg cgcgcctgat gcgcggctgc ttttcccgcc gcttgggcgg
     1501 cttgtttttg tttgaggggg tgtttttgaa aaaccgcctg cattcaggcg gttttgtttg
     1561 cttaggcgta tgcctgcaat ttggggttga tttcggtttg ggcgaggttg ttggcgtagt
     1621 tgcacaaagt tgccaaggct acgcccatta cgacttcgac tgcctgctgc cggttgtagc
     1681 ccgcatcgaa aaatgctttg agttcgtcgt cggatactgc gccttttttc gccattacgg
     1741 cttgggtaaa ggcggcaagc gcgccgagtt tggcatcgtc aaattcacct gccgccaaag
     1801 cgcgggcggc attgagggat tgctcggaca ggagtttttt cagggttgcg agtttggtgt
     1861 gccctgccac gcagaagccg cattggttgg tgcgtgcggc aatgatccgg atcacttcga
     1921 cttcgccggc ggtcaggctg ttggcggcgt tgagcttgcc gacttcttgg taaaacgcca
     1981 aggcttcggg ggcgtttgcc aatacgccga tgaggttggg gataaagccg ttgtttttga
     2041 gtacggcctc tacgcgcggt ttggcggctt cgggggcggt ttcgagggtg tgtacggtta
     2101 aacgtgccat tttctttcct tgttcgcaaa tattggggac gggcgcatgg tacgcatttc
     2161 ggaacggaat aggaaagact gtgccacaaa caaaaagtta taagaaatgc cgcctgaagc
     2221 ctttcagacg gcattgccgg gaaacacgcc tacccgcccc cgcgtccgaa atattgcgcc
     2281 aatgcggctt gggggttggc ggcttcgact gtttcgggca attcggtata gccgcgcgat
     2341 aaaaggtggc gtgcataaag gctgttgccc tttgcgccga accagacgga aaccgccaag
     2401 cccaatacgt taaacgccga gtctgtggcg ggcgtgtcgc cgtaaaccag ttgggcaaac
     2461 accgccaaca cgaatatcgc gcccgtcatc cccagcccca aaccccacat tcttttgaaa
     2521 cacgcccaca gcgtgccgaa caaaagaccc ggccatgacc agccttgttt gacggcttgg
     2581 ggcggcaggg cgggatgggt gtagattttg tatggtttca tcgtgtttcc ttttcggttg
     2641 aaaccccgcc ctttggggcg gtaggatcag actttatttg tgagacgcgc gcccgtgtgc
     2701 agacatacgg cttgaacatg cccgtcgggg aagacagatc cgacaatgcc gtctgaacgg
     2761 tgttttgcct tcagacggca ttgcctgcct tcaaagcgga cgcgcttatt ccgcccagtt
     2821 tttctttttg ctggttttgc cgatacccgg gttgaagctg ttggtcgggt cgagtttgcg
     2881 gtaaaactgt ttaagcgcgg gcttggcttc atacaaatgg ccgacgttgt gttcggcggg
     2941 atattgcgcg ccgcgttggt ctaagagatg cagcatttcg tgttccaacg ccatacagcc
     3001 gttgcctttt ttgatgatgt aatcctgatg gaaaacgtgg cacataaaat gcccgtaata
     3061 caatttatgg atgattttat tgtcgatttc cggcggcagt ttttcaaacc agtcgcggtc
     3121 gtcgcggcgc agggcgatat ccagcgcgac caagtcctcc acttcgtcgt cgtgcacgga
     3181 acggtagcgg atggcggcgg aggcgacggc gaaacggtgc agcatggcgg cttgggtttc
     3241 ttcggcgttg cattcgaaaa acgcgccgcc gtggtgtgaa aaatattctt ttaagaacgc
     3301 gcgcgcctca tccacgcctt ttccgcccat tttcagaatc aggtggtgtt cgtatttgtc
     3361 gcggtaatcg cgcatggatt tgggcaggtg gtcgggcagg aatttgctga cgaactgcat
     3421 tgccttgtcg gaaaaatgtt tgggcaggaa gctgactttt ttgccgaacc tgtccacgcg
     3481 cgccttcaag tcaaataatt tcggcagttg gtgcgtaccg aattttttga tgacgtagaa
     3541 cgtgtctttg ccgtacacgt cggcaatgtc gaaagcgtgg cggtggatgt attcgccgga
     3601 aacgggcagg ctttcaaatt cgcccaaggc ggcgcggcgg atgtcggtca gctcgttgat
     3661 gtcgttcgtg ccgatgtaga acacggcggt ttgtttttct tgcgggaagg tgtccaaacg
     3721 gacggcaaaa accatcagct tgcccgcgca gcccgaggct tcgtaatggc gggcggggtc
     3781 ggcattgaaa cgcgcggcgg tcggttcgtc cacttggcgg acgtgttcgc aataggcgtg
     3841 gtcatgtcct ttgcccgcgt cttgtttgat gtcttttttc tgataatgat gaccttgcag
     3901 gttggtcagg atttcttcgg gcgtgtcgcc caagtctatg cccaagtggt tgaccagttc
     3961 caacctgccc tcttcgttga tttgggcgaa caacgccatt tcggtgtagg ccgggccgcg
     4021 ctgtaccaac gcgccgccgg agttgttgca cacgccgcct aaaacggacg cgccgatgca
     4081 agaagagccg atgaccgaat gcggttctcg tcccaaaggt ttcagcagca gttcaagctg
     4141 gttcagggtc gagccgggca ggcagacgac ttgttcgttg ttgttgatgg tttggatgat
     4201 gttcatccgc atggtgttca caatcacgat gtcgcggtcg taatcgttgc cgtcgggggt
     4261 cgagccgccg gtcagtccgg tattcgccgc ctgcgtaatc acaatcacgt ccgcttcgac
     4321 gcaggcctgc agaattttcc acatttccaa aatgcttccc gggcgcacca ccgccaacgc
     4381 cttgccttcg ccgaaacggt agccttggcg gtattgttcg gttttcgcgg ggtcggtaat
     4441 gatgtatttt tcgcctgtga tttgggtcag gctgctgatt aattgcgagg cgttcataac
     4501 actctcctat cgggataagc ggcggcgcaa atcccggtac gccgccgtat gttcaatttg
     4561 gatattttgg tgcggcaagt tgcacgctgc aaattatttt tggttaaaga aataatgtaa
     4621 tttaatattt ttggcatttg ccgcgttttc caaatcggcc ttgtcgcttt cgctatcgcc
     4681 cgtcatatag acactgacat tatttccgac tttctttgca tggtttaagg aataaagtgc
     4741 ggtcgcctta ttaaacattt ggctgcctcc cgccacgccg acgttgatat tgtccgcgcc
     4801 gagcatttca atcatatttg atgccgaagg cgatccggat gttttaatca ttccgacagc
     4861 caccgcatcg gaaagcgggt tattcgtcgc gggaacctgc acgacaacag agcgttctcc
     4921 ctgcggcatt tcaaccatca accggccgac attgacacct gttgaacaag ctgcaagcat
     4981 accggtgagg gttatcatta gaagtttttt catttgccta ctcctttagt ctttaatcaa
     5041 accgccctgt tcgtcatata acttgaaatt gaaccatact tccattttta tgaagccggt
     5101 tcttcattgg cctgtatgga tacattgtaa tgatgcgtta tggcaaagta aaggaaatgc
     5161 cgtctgaacg gctttcagac ggcatcggtg cgatacggga acgccggaac atcgaagctc
     5221 cggcgtttca aatagggcgg cggaccaaac ccccggcact ggcgcatcgg agtgggctgc
     5281 tggcttccgc ccctgacccg gtgttccgat ttgccatgcg gggagacccg cctcggagaa
     5341 acggcattat aacgggtttt ctgaaaaact caaccgtttt gatacggtca tacgccggaa
     5401 acaccgccta aaatttatat ttgataatat tgtcaacaat ttctcaaagc gttattttgt
     5461 ttctataagg gtattgcctg tttcggcatt gaaaaatatc aaaaattgaa ctacattatc
     5521 gccttttcaa acccggctga aaccgacttt tcaaacggca ttcaaataaa aactgccaaa
     5581 cacggacaca ctatgaccac gactaccgcc ccgcagcgta ttcgggaaat cccctacaac
     5641 tatacttcct acaccgaccg cgaaatcgtc atccgcctgc tgggcgacga ggcgtggcac
     5701 attctacagg acttgcgcgg acagcgcaaa accgggcgtt cggcacggat gctgtttgaa
     5761 gtgttgggcg atatttgggt ggtcgtacgc aatccgtatc tggtcgatga tttgctggag
     5821 catccgaaac gccgcgccgc gctggtgcgg gaaatgcgcc acaggttgaa cgaaatccgc
     5881 aaacgccgcg acgataatca gcaagcggat gtgttgattg cggcggcgga aaagggagtg
     5941 gagctttttg acggcagctt tgacgaaacg cgccaaaagc ggcggcagat tttggagcgt
     6001 ttgagcaaaa tcaccaagcc gcacaatatt atgtttgacg ggctggcgcg ggtaacacac
     6061 gttaccgatg cgaccgactg gcgcgtcgaa tatccgtttg tcgtcgtcaa tcccgacacg
     6121 gaggcggaag tcgcgccttt ggtgcgcgcc ttaatcgagc tggacttagt tattatcccg
     6181 cgcggcggcg gtacgggtta taccggcggc gcggtacctt tggacgcaaa cagcgcagtc
     6241 atcaataccg aaaaactcga caagcatcgc agcgtcgaat acgttgagct ggcaggcttg
     6301 tacggcagac atccgattat ccggtgcggc gcgggcgtgg ttacgcggcg ggtggaagaa
     6361 accgcgcatc aggcgggctt ggtgttcgcc gtcgatccga cttccgccga cgcgtcctgc
     6421 gtgggtggta atgtggcgat gaatgcgggc ggtaaaaaag ccgtattgtg gggtactgcc
     6481 ttggacaacc tcgcctactg gaacatggtt aaccctcaag gcgaatggct gcgtatcgaa
     6541 cgcgtgcgcc acaatttcgg caaaatccac gacgaagaaa ctgccgtgtt cgacgtgcac
     6601 acgctggatt cagacggcat caacatcgtt aaaaccgaac gcttggaaat ccccggacac
     6661 aaattccgca aagtcggttt gggcaaagac gttaccgaca aatttttgag cggactgccc
     6721 ggcgtgcaga aagaaggtac ggacggcatc attaccggcg ttgccttcgt attgcacaaa
     6781 atgccgaaat acacgcgcac cgtgtgtatg gagtttttcg gcacggccgc caccgccacg
     6841 ccttctatcg tcgaaatccg cgattttctg ctcgcacacg acagcgtgcg gctggcgggt
     6901 ttggagcatt tggactggcg ttatgtccgc gccgtcggct acgccaccaa agcggcgggc
     6961 aaaggacgac cgaaaatggt cttgctggcg gacgtggttt cagacgacga agccgccgta
     7021 gaggcagccg ccgaacacat ctgcgaactt gcccgcgccc gtgacggcga aggctttatc
     7081 gccgtatcgc ccgaagcgcg caaaaccttt tggctcgacc gcagccgcac cgccgccatc
     7141 gccaaacaca ccaacgcctt taaaatcaac gaagacgtgg tgattccgct ggagcggctg
     7201 ggcgagtatt cggacggcat cgaacgcatc aacatcgagc tttccatcca aaacaaactc
     7261 aaactctgtg ccgctttgga gcaatatctt tcgggcaaac tccccatcga caaaatgggc
     7321 accgacctgc cgaccgccga actgttgggc gaacgcggca aacacgccct cgcccacgtt
     7381 gccgccgtca aagcgcgttg ggactggctg ctcgcccatt tggatgcgcc gcttgccgac
     7441 tacaaatcac gctacggtac agccgtccac gccgcgcccg aagccaaaga cgatgaaagc
     7501 tgcttcaccg ccttccgcga tttccgcctg cgcgtgtctg tcaaagcaga cgtaatgaag
     7561 ccgcttgccg aaatcttcag cggcaaaacc gataccaaaa ttatccaagg cttgggcaaa
     7621 atccacgcca aaaccgtacg cagccgcgtc tttgtcgccc tgcatatgca tgccggcgac
     7681 ggtaacgtcc acaccaatat tccggtcaac tcagacgatg ccgaaatgct tcagacggcc
     7741 taccgttctg tggaacgcat tatgaaaatc gcccgttcgc tcggcggcgt gatttccggc
     7801 gaacacggca tcggcatcac caagctcgaa ttcctcaccg atgaagattt gcaaccgttt
     7861 tggaactaca aaaaccaagt cgaccccaag cacaccttca accgccacaa actgatgaaa
     7921 ggttcggact tacgcaacgc ctacacgccg tctttcgagc tgttgggcgc ggaatcgctg
     7981 attatggaaa aatcagacct cggcacgatt gccgattccg tcaaagactg cctgcgctgc
     8041 ggcaaatgta aacccgtctg ctccacccac gttccgcgcg ccaacctgct gtacagcccg
     8101 cgcaacaaaa tcctcggcgt gggtttgctg accgaggcct tcttatacga agaacaaacc
     8161 cggcgcggcg tttccgtcaa acattttgaa gaactgatgg acatcggcga ccactgcacc
     8221 gtgtgccacc gctgcgtcaa accctgcccc gtcaacatcg acttcggcga cgttaccgta
     8281 gccgtccgca actaccttgc cgattccggc cacaaacgct ttgcgcctgc ggcttcaatg
     8341 ggtatggcgt ttttgaacgc caccggtccg aaaaccatca aagccctgcg cgccgcgatg
     8401 atacagaccg gcttccccgc gcagaacttt gcctacaaaa tcggcaagct tcttccgatc
     8461 ggcacgaaaa agcaaaaagc cgaacccaag gcaaccgtcg gcaaagcccc gattaaagaa
     8521 caggttatcc atttcatcaa ccgcccactg cccaaaagcg tacccgccaa aacaccgcgc
     8581 tccttattgg gcatcgaaga cggcaaaagc atccccatca tccacaaccc cgccgcgccc
     8641 gaagatgccg aagccgtgtt ctacttcccc ggctgcggtt cggagcgttt gttcagccaa
     8701 atcggactcg ccgtccaagc catgctctgg cacgtcggcg tacaaaccgt cctgccgccc
     8761 ggctatatgt gttgcggcta tccgcaagac gctggcggca acaaggcaaa agccgaagaa
     8821 atgagcacca acaaccgcgt cgccttccac cgtatggcga acaccctcaa ctacctcgac
     8881 atcaaaaccg tcgtcgtcag ttgcggcact tgttacgacc agcttgaaaa ataccgcttc
     8941 gaggaaatct tccccggctg ccgcatcatc gacatccacg aatacctgct cgaaaaaggc
     9001 gtgaaactcg acggcgtgaa aggtcagcaa tacctctacc acgacccctg ccacaccccg
     9061 attaaaacca tgaacgccac ccaaatggcc agcagcctga tggggcagaa agtcgtttta
     9121 agcgaccgct gctgcggcga atccggtatg ttcgccgtca aacgccccga catcgccaca
     9181 caggtcaagt tccgcaaaca agaggaaatc gagaaaaacc tcaaagagct gccgcagggc
     9241 gaacccgtca aaatgctgac ctcctgcccc gcctgcctgc aaggactgag ccgctattcc
     9301 gacgacaaca atatgcctgc cgactacatc gtcattgaaa tggcgaaaca catcctcggc
     9361 gaaaactggc tggatgagtt tgtaaaaaaa gccaacaacg gcggcgtaga gaaagtgttg
     9421 ctataacaac ggacacggaa atgccgtctg aacgccgaaa gccttcagac ggcattgttt
     9481 gaatcaaata cctacccgaa tcatgattga cggacaaaca accgaaccca aacaaaaaac
     9541 cagaatcata ccagccccca tgcgggggct ggtagacgat gtgatgcgcg acctgctgac
     9601 acgtatcggc ggctacgacg aatgcgtcag cgaatttgta cgcattaccc ataccgtgca
     9661 ttcacgatcc atatggttaa aatatgtccc cgaaatcgcc aacggaaaca aaacgttttc
     9721 cggcacgccc tgcaccgtcc aacttctggg cagcgatgcg gacaatatgg cggcaaacgc
     9781 attggaagcc gtccgattcg gcgcggacaa aatcgatttg aacttcggct gccccgcgcc
     9841 caccgtcaac aaacacaaag gcggcgcaat tcttctgaaa gagccggaac tgatattcca
     9901 catcgtcaaa acgctgcgcg gacgtttgcc tgcacatatt ccgctgacgg ggaaaatgcg
     9961 gctaggctat gaagacaaaa gccctgcttt ggaatgcgcc tgtgcgattg ccgaaggggg
    10021 cgcgtgcgga ctgaccgtgc acgcgcgcac caaagccgag ggctacgaac ccccggcgca
    10081 ttgggaatgg ataaggaaaa tccacgatac cgtcaatatt cccgttaccg ccaacggcga
    10141 cgttttcagc ctgcaggact atatcggcat caaaacaatc agcggctgca acagcgtgat
    10201 gctcggtcgc ggtgcggtca tccgccccga tttggcgcgc caaatcaagc aatacgagaa
    10261 cggcggaccg gtcaaagaca cggattttgc cgaagtttcc acatggatag ggcagttttt
    10321 cgagctgtgc ctgacaaaag aggcaaacaa caaatacccg atcgcccgtc ttaagcaatg
    10381 gctgggcatg atgaaaaaaa cgttcgaccc ggcgcaaacg ctgttcgacc gcgtccgaac
    10441 ggttaaagat gcggatgaag tgcgccgaat cctgaacgca tttgagcatg agatggatgt
    10501 ttgatgtctc ccgcgcttca aagcaaatgc cgtctgaaaa cctttcagac ggcatttgtt
    10561 atctttattg ccgtttttcc cctgctttcg gtgtattttt gtttttcagt ttcgccccca
    10621 aacccaaacg tttttcataa tccgattgcg gggtatcgtc ttcctgcata ccgaacgcgc
    10681 cggcattgac ccacagcgac agcagcgcga cgacaaaggc gcaaaagccg attacatacc
    10741 aaaacattgc ccctccaaat ttgttaaaat catatcaaat acagtgccga atttatcaca
    10801 agcgcacgag caaataaatc ttctgaccgc agtatttcat tcatttacct tgaaaacaaa
    10861 aaagggcacc tgaaaatttt gcgaaacttt tcagatgccc tgctttgccg aaaaacgtat
    10921 cagccgcagc aaccgccttc gccgtgtccg ccttttttgt cgttatcgac aatgggcagt
    10981 tcgacttttt cgtttggttt ttctgcattt tcgggccggt cgttgagttt gattttcgcg
    11041 gtttcgctca tttccttacc tcatttggaa aacggtttga aagattcatt aatatagcat
    11101 gtttaatccg aatctgaaaa gggaagccgt atgcgaaatc ccgtctgaag cccaatcagg
    11161 gcttcagacg ggatttgcta tctttattgc cgcttttctt ccgtatccgg atttttgttt
    11221 ggggctgaag cagattggca gtcagattgc aatcgaagaa tgaaggcaag ccatcaaaaa
    11281 caaagctatc cgcttcaccg ccccgatatt tagaatttgt ggcgcagacc aacggaggcg
    11341 gcattaattt gagtgtagtt gccgatgccg gtatttcgtt tcagccaagc ggcagacatg
    11401 atggcggaag tgcgcttgga aaaatcgtaa tcgacaccgg cgatgatttg atcatagctg
    11461 gtatgttcgc gtttctgact gcgttcgaca aagtcgaaac catgggcata gctgatgcgc
    11521 gggactgtat taccgaagcg gtaggaagca gtggcggcaa tttcggtcgt actgtttttg
    11581 gttttgtcgg cattttcaga caaatccaac tgagccgcca aggcgagatt caagccgctt
    11641 cctcatagcc gcccgtcagg cggtgtacct gatggttttt caatggatcg gtatcactcg
    11701 ctctgccgag caagaacaaa aagaaagcat catgcccctc attggcgtgt ttcgcatatt
    11761 taagggcata atttccgaaa aagccgccat ttttgtaatt cagaccggca taatacacat
    11821 ccgaccccgg cttgccgaca acagccggaa ctggtttcat ctgattactt tccagcgtga
    11881 aagtagcagg cgtataggcg gacttgctgt tttgactcgg aacaaattga atgctgccgc
    11941 tgaaaccgga aaatccgggg gaatcgtaac gcacggaaac cggcataccg tcgtggcgtt
    12001 tgaaaatacc caattgcgaa gccacattat tattgctgtc ccaaggatca atggctttgc
    12061 tggcatcgcc aaacggattc gcaacgcgac cggcgagcgc cgtgccgaat tcgcctgcca
    12121 agccgataaa ggattccctg ttaccccaac gggtcgcgcc gccgccggca acggatacgt
    12181 cttgctcgag ctgccaaaca gccttcagcc cgccgcccaa atccccaccc ccttaaagcc
    12241 gataaacgag ccgaaatcac tgactttcgt cctgatgcgg cttttggcct tagtaactgt
    12301 attgcccgtt tgaccttctg agggtggctc ggtcaactgc agccggatgt tcctgccttc
    12361 cacaccagct ttgacttcgc cgtacaggct gacatcggca actgccgcaa acggcagtgc
    12421 ggacaatacg agggcggtaa gtttttttcg catatcggct tccttttgta aatttgctaa
    12481 aaacatcggg caaacaccca atcagtctcc aattaccccc cgaaaaaaaa accatttttc
    12541 ggaacaaatt tcgggtaaat tttggaattt ttattttgaa aaataatatt tcaatataaa
    12601 acaataaatt aacttttttg aaaatgttgc tttcccgcaa catgatttaa atacagacct
    12661 tgtcaaagcc gatgcggcgc agacccggat gccgtctcgt tttttaaaac accataaaaa
    12721 aacacacgcg gtgctttaat ttcctgcgtg tgtcgagtgt atcgaaatca agccgaatca
    12781 aatataccgg acttcgataa tgccgtattc gcgtacgccg cccggtgcct gaacttccgc
    12841 cgtatcccct tcttccttgc cgatcagggc gcgggcaatc ggagagccga catagatttt
    12901 gccctgtttg atgtcggctt cgtcttcgcc gacaatttga taggtaacgt gttcttccgt
    12961 ttccaaatct tccagcgtaa ccgtcgtacc gaacacgatt ttgccttcgg cgtggatttc
    13021 ggccggattg atgatgtggg caacggaaag tttgtgttcc aactcggaaa tgcggccctc
    13081 gataaaacct tggcgttctt tggcggcttc gtattcggcg ttttcggaca aatcgccgtg
    13141 cgatctggct tcggcaatcg cttcgatcac ttcgggacgc gccacgcttt tgagctgctg
    13201 caattcctgt ttcagcaatt ccgcaccgcg tacggtcagg gggatttttt gcatcggtct
    13261 ttttctccat attccggcac accggtttgc ggcggcaagc ataccgcgta ccgtcttgtt
    13321 ttgtgcgtcc ggatattaaa ataaaaatac aagccgcccg gaaaatcggc ggcttgtctg
    13381 tcgttgaaca gcggctattc taccaaattc tatgaaattg gcaatcgtgc cgtgccgccg
    13441 gcaaacgcgc tatgtccgca acaaaagccg aaaatatgcc ggcaaaaaaa ttttagaaac
    13501 aaaaaattta aaaaaaatca attttcggca taaaaaacca aattcacgta ctttaaaacc
    13561 gaaaatgcca aacctgagat tttccagacg gcatttgcac cagtataatg caggctgttt
    13621 ttatctttat atttacgttt tgccatgacc gaatccgtcc gcctccccgc cgcctcgctc
    13681 aaaccttcca ccgtcgccct gcccggctcc aaaagcatca gcaaccgcac cctgctgctc
    13741 gccgccctgt ccgacaatgt ttgcgaaatc cattccctgc tcaaatccga cgataccgac
    13801 cgtatgctcg aagcactcga taaactcggc gttcaaatcg aacatcttgc cgaaggccgt
    13861 ctgaaagtac acggcacggg cggacgcttc cccaaccgct ctgccgattt gtttttgggc
    13921 aacgcgggca cggcgttccg tccgctgacc gccgcgctgg ccgttttggg cggcgattat
    13981 catctgcacg gcgtgcctcg tatgcacgaa cgccccatcg gcgatttggt cgatgcgttg
    14041 cggattgccg gcgccgatgt cgaatatctc ggcaacgaac actatccgcc gcttcatatc
    14101 ggcaaacgtc aagactgcgg cgagcgcgtg attcctatta aaggcaatgt gtccagccag
    14161 tttctgaccg ccctcttgat ggcgctgccg ctgaccgggc aggcgtttga aatccgtatg
    14221 gtcggcgagt tgatttccaa gccctatatc gacattactt tgaaactgat ggcgcaattc
    14281 ggcgtacagg ttgccaatga aggctaccgc gttttcaaaa tccctgccga tgcgcactat
    14341 cacgcacccg aacacttgca cgtcgaaggc gatgcctccg gcgcgtccta cttcctcgca
    14401 gccggtttga ttgccgccac gcccgtccgc gttaccggca tcggcgcaaa cagcatacag
    14461 ggcgatgtcg cctttgcccg cgaactggaa aaaatcggtg cggacgtggt ttggggcgaa
    14521 aacttcgtcg aagtctcgcg cccgaaagga cgcgccgtcc aagcctttga tttggatgcg
    14581 aaccatatcc ccgatgccgc gatgaccctc gccatcgtcg cgcttgccac aaggcaaacc
    14641 tgcacgcttc gcaacatcgg ttcgtggcgc gttaaggaaa ccgaccgcat cgccgcaatg
    14701 gcgaacgagt tgcgcaaact cggtgcaaaa gtcgtcgaag aagccgaagc gattcacatt
    14761 accccgcccg aaacgccgac acccgatgcc gtcatcgaca cttacgacga ccaccgcatg
    14821 gcgatgtgtt tctcgctgat ttcgctgttg ggagtacccg tcgtcatcaa cgacccgaaa
    14881 tgcacccata aaaccttccc gacttatttt gaagtgttct catcactgac cgaaacagcg
    14941 gaataaggcg gaaaaatgcc gtctgaaacc cgaaaacagg gcttcagacg gcattttgcc
    15001 gcgattccgg cgcggcgggc ggctcattct gtaaaaaacg tatgcgctcc aaggtagttt
    15061 ttggcgtaaa acggtgtgga gagtttttcg gttttgatgg ttttgccgct gccgggggca
    15121 tggatgaatt cgccgttgcc gatgtagagt ccgacgtgtg agtagcggtg tgcgccgccg
    15181 gtgttgaaga atacgaggtc gccggccttg aggcggctgt cggggatttt gcggcttgcc
    15241 gccgccatgt cgcgggcggt gcgcggcagc ttgacgttga gggcgttttt gtaaacgaat
    15301 tgaatcatgc cgctgcagtc gaagccggtt gcggtgctgc tgccgcccca tttgtagggc
    15361 gtgccgatga gtccgaggct gtggagcatg agttcctgcg agccttgtgt gcggccgatg
    15421 tggctgatgc ggacggcttg gatttgccgg actgtctgtt tgggtttcgg ttggcggtgt
    15481 ttgccggagg tcgtgccgca tgaggcgagg agcagtgcgc cgagacagag gaaaagggtt
    15541 ttgtcggggg aaaacatggt tttttccttt gcgggttcgg atatgccgtc tgaaggtgtt
    15601 tcagacggca tttctataat aaaccttcga tgggcagcag ggataggatt tttgcggcga
    15661 tgcgtttcca aagtttggct tcgggttcgt tcgggtaggt ttttcgggtg gcgggatcgt
    15721 gccattgcag gcggttgtgt ttgtcgaggg taacgcggta ggcgtattcg ggtgtggtat
    15781 cggcgagggt gcgctccatc tgttctgcga ttttggggct ttcgatgacg acgcccattt
    15841 cggtattgag ccgtgcggaa cgggggtcga ggttgaatga gccgatgaag atgcgtttgc
    15901 cgtccacaat gaaggttttg gcatgcaggc tggttacgga gctgccggtc aggcctttgt
    15961 cttttgtggc ggggacggca tggttgggtt gcagctcgta gagtttgatg ccggctttga
    16021 gcagcggttt tcggtatttg acgtagccgg aatggacggc ggcaacgtcg gtcgcctgta
    16081 gcgagttggt caggacggta acgtctatgc cgtcctgcac cagttttgcc agtgcgtctg
    16141 tgccggattt tgtagggacg aaatagggtg aaaccagata gacgcttttt tcgggctgtt
    16201 tgagcgcgtc ttgcagcctc ccggcaatcg gcggtttgcg gcggtcgcgg tcgagtcctt
    16261 ttgcagggtt gtcgctgatc aggcgggttt ggacgctctg ccagtcgatg cgtcccgtct
    16321 gtattttttg gtagaggggc gactgttcga cggtttcgcg gtagcgcagg agcgcgtgtc
    16381 tggatgtttc gtcgttgtat ccgagtgctt gaagaccctt gccgatgttg ccgctgcgga
    16441 tgatgcgcgt ggcgttgtgg gcggaatggc ttgcccagta gcggtcgaag tcgtgcgata
    16501 cttcgccgac gacgctgccg gtggcgagga tgtccaggtc ggcgaaaacg gtgtcctcac
    16561 cgactttgaa gtattcgtcg ccgatattgc gtccgccgag tatggtggcg cggttgtcgg
    16621 cggtaaagga tttgttgtgc atgcggcggt tgaggcgggg gaagtcggtc aggtagccga
    16681 gtgcgcgcca tttgcgtagg acgaaggggt tgaacaggcg cacttcgata ttgggatggc
    16741 tgtcgagggc gagcaggaga tcgtccaacc cgcgcgtgtt gttgtcgtcc aacagcaggc
    16801 gtacgcgcac gccgcgttct gcggcaaggt acatgaggtt gaacagcagc ctgccggaaa
    16861 tgtcgttgcg ccaaatgtag tattgcaaat cgaggctgtg ttcggcagat tcgataaggg
    16921 cggcgcgggc ggcaaaggct tcgtgggggt cgtcgagcag gtagatgtcg gatagcccgt
    16981 tgttatgagg ggtgtgccgg atttgcagga tgttgtccag gaggacaggt ttggaagtat
    17041 tgaaatgacg gctttccgtc cgttcttcca gtgggggcaa ccatgaagaa catgaacaga
    17101 gaaggaggca taaaagggaa atgaggctgc gtgttttcat cggggatata gtttcagacg
    17161 gcattgcctg tattttgggg tctgtatgca ttgaaatgaa gcatcataat ctaaaggttg
    17221 gaatgggtaa aagttttgtc gggatgcggg gttaaggccg cgtgcctctt gtcaggtttg
    17281 caccgtctga aacgtgcaga cgcttaaaaa ccaacgacga ggcaagggtg atgccgccga
    17341 tactgagcag ggtcatacgg aaggcgaaat gcaggcctga agaagccggt atcagaaatg
    17401 tccagttttt aaggattaat gcgccggcaa caatgcccac gctgatggca agctgttggt
    17461 tgaccgccat caggctgttg ccgctgccgg tctgttgcgg gcgcaaatcg gcgagggtca
    17521 gtgtgttcat ggcagaaaac tgtagggagt tgcatgcgcc gatcgccagc gagaggaaaa
    17581 tccaaatcca cagcggcgag tttccgtcag gcagggcgag cagcatgatg aaggcggcaa
    17641 gcagcttggt gttccaaagc agtaccgtgc ggtagccgaa acgtttcatg agcggtgcaa
    17701 tcagcggttt gaccagcagc gaagacaggg cgacgggtgc gaccagccag cccgacaggc
    17761 ttgcgccgaa gccgaaagcg acttgaaaca tcaggggcat cagaaaagga atcgagctga
    17821 tgccgagacg gctgaacaga ttgcccgcca gtcccaaacg gaaggtgcgt atcagaaaca
    17881 ggtcggcgga ataaatcggt ttggacgcgg ttttcatatg tcggaaataa cggcgtgcaa
    17941 acagcagtcc gccgcacagc ggtaacagtg caaaatacgg aggcagcgcg tgcgacaggc
    18001 tttctgccgc aagcagcagc aggcaggcgg caacggaaaa ggtcagatag cctttgaaat
    18061 ccagagcggt atcgttgcct ttgacatcgg gcatgatgtt gcgtcccaat acgaaaccca
    18121 gcagaccgat gggcaggttg agcaggaaaa tccagtgcca cgaagcgtat tcgaccagat
    18181 aaccgcccgc cagaggtccc aacgcagggc cgatcagtgc aggcatgacc gcataattga
    18241 tggcgttgag cagcttcgat ttttcatata cacgcaagat ggtcagacgc ggtatcggaa
    18301 ccagcatcga accgccgatc ccctgaacga cacgggaaag cgtcaattca aacagcgaac
    18361 ccgatgcggc gcacaatgcc gatccgagca taaaaacggc aatcgaaccg aaaaagactt
    18421 ttttcgttcc gaacttgtcc gccaaataac tgctcaaagg aatcagcagg gcaaccgtca
    18481 gcgtataggc aacgactgcc tgttgcatat ccggaggcga ttcgttcagg tcggcggcaa
    18541 tttcgggcag tgcggtattt aaaatggtcg catccaacat ctgcataaaa atggcaattg
    18601 ccagcagaag cggcagccaa ggggatggtg cgcgggcgga cagggtgttt ttttccatag
    18661 ggtgattgta ccccatcctt gtgccgttat tgttttcaga tgctgtctga atgccggcag
    18721 agtcggcatc ctgaatgttc acaagcaaac gaatcggcat tgcattgtaa tgataattat
    18781 tatcgaaaat catcagagta aggtacagta agcgttatgg ggcagtttgt aagaaaaacc
    18841 ggattatttt ttaaaattag acttgacccg caacggtcaa ttacttaaag taaacgctta
    18901 cctttctaca gaaaaaacgg gtctcccgtt atcaaaaaat atgagcgcaa ccattccccc
    18961 aaaaatcatc cgatacgaca gcaatccgac agatgtctat tttttcggca cttgcgtcct
    19021 tgatcttttt atgcccgaag caggcatgga tgccattgcc ctaatcgagc agcagggcat
    19081 acgcgtccat ttcccgatgg cgcaaagctg ctgcggtcag cctgcctatt catccggcca
    19141 tccgaccgaa gccttcgatg tcgccaaagc gcaactcgac cttttccccg aaaactggcc
    19201 gattgtcgta ccgtccggct catgcggcgg catgatgaaa catcactggc ctaccctgtt
    19261 taaaaataca gaatacgaat ccaaagcggt tgattgtgcc aaccgtatca ttgagtttac
    19321 tcatttcttg cttgccatcg gctacaagcc tgaagacaaa ggcgagccag tcaaagtcgc
    19381 cgttcacact tcctgtgccg cccgccgaga aatgaatgtc catctttcag gctggcagtt
    19441 gattgacggt atggaaaacg tcgaacgcat cgtccacgac cacgaaagcg agtgttgcgg
    19501 cttcggcggc acattctccg tcaaacaagc cgatatttcc ggcgcgatgg taaccgacaa
    19561 agttgccgcg ctgaaagaaa ccggcgcaac cgaaatcatc agcgcggact gcggctgtat
    19621 gatgaacatc ggaggcaaaa tcgccaagga cgagccggat atgccgcgtc cgaagcatat
    19681 cgcatccttc ttgttggaac gcaccggagg caaagtatga gcgcgcgcga aaatattttg
    19741 gcaaaactga aaaaagccgg cgcattgccg atggaagagc ctgcggtttt tgattattac
    19801 cgcgaaaagg gcgtttcttg ggacagcgaa gccgagcgtc tgaaacattg ggctgccgcc
    19861 atgcgcgcgg tcaaaaccga aatctattgg gtgacgaaaa gcaattggat gcaggttttc
    19921 cgcgaagcgg cagaaggcaa gggtttaaaa aacatcctgc tgcccttggc aaccgaacac
    19981 ggacaaattg cccgtgccgc attggcgggc agcaatatcg acccgattgc cttcgagcgc
    20041 gaaatcgata cttggaaaac cgagtttttc acgaacatcg atgcaggctt cagcggcgcg
    20101 caatgcggca tcgcccgcac cggcacgctg atgctgtttt ccagccctga agaaccgcgt
    20161 actttgagcc tcgttccgcc cgtgcatttc tgcctgttcg atacgtccaa gatgtacaac
    20221 gagtttcaca acgccgtcga aggcgaaaaa ctggtggaaa acggtatgcc gaccaatgta
    20281 ttcctgattt ccggcccgtc caaaaccgca gacatccagt tgacgctcgc ttacggtgcg
    20341 cacggtccgc gtgatttggt catcctcgcc attttgcccg accacatttc ccctgccgat
    20401 ttggaggaaa acgcatgact acgcaaacca tcaagttcca catgaagccg gaaactttca
    20461 aacaaaacgc cgcaatttcc cttcaagaca agcccttgcg taaaagcctg cgtaccgcga
    20521 tggatatgct gatgaccaag cgcaaagccg ttttgaccga cgaagaagag ttgcaaagcc
    20581 tgcgcgattt gtgcgaacac atccgtcagc gttccctctc caaattaccc gcgctgctgg
    20641 agcagttgga agaaaacctg actaagttgg gcgtgaaagt acactgggcg gaaacccctg
    20701 ccgaagcctg ccaaattatc cacgacatca tcacagccaa aaacggcaag ctgatggtca
    20761 aaggcaaatc catggtcagc gaagaaatcg agctgaacca ttatcttgaa gcaaaaggca
    20821 ttaaggcggt ggaaagcgac ttgggcgagt ttatcgtcca aatggcaggc gaaaaaccga
    20881 cccatatcgt gatgcccgca atccataaaa ccaaagaaca ggtcagcgaa ctgttccacc
    20941 aaaacctcgg cacaccgctt acagacgacg tggaccaact gaccggattc gcccgcaaag
    21001 ccctgcgcga tatttacagc actgccgatg tcggcttgag cggcgtaaac ttcgccgttg
    21061 ccgaaacggg cacgctgtgt ctggtcgaaa acgaaggcaa cggccgcttg agtactaccg
    21121 ttccgcccgt acatatcgtc attacgggta ttgaaaaagt tgtcgccaag ctgtctgatg
    21181 ttccgccttt gtacagtctg ctgccgcgtt ccgccatcgg tcagaacatt accacttatt
    21241 tcaatatgat taccggcccg cgccgcagtg aagaattaga cggtccgcag gaaatgcact
    21301 tggttttgct cgacaacggc cgcagccaag cttatgccga agaccaaatg cgccgcaccc
    21361 tgcaatgtat ccgttgcggc gcgtgtatga accattgccc ggtttataca cgcatcggcg
    21421 gcgcggcata cggcacaacc tatcccggtc cgattggcga gattatttcc ccgcacctgt
    21481 tgggtcttga tgccacccgc gacctgccga ccgcctgcac gatgtgcggc gcgtgcgtgg
    21541 aagtttgtcc ggtacgcatc ccgattaccg aacaaatgca acgtttgcgc gttgaagcac
    21601 aacgttcgcc gaccgaaacc gtgccgcacc ccatccgagg gcaaggcgca tcgcatacct
    21661 tcggcgaaca aatggcgtgg cgcacattca acggtatttt cagcggtagc aaaacctacc
    21721 gcgccttcgg ttgggcagcc accaagttcc gcaatctgac cccgcgcaaa cagttgggtt
    21781 ggacgcaaaa ccgcgtaccg atgaaaccgg cgaagaaaac cctgcacgaa ctgatggcgg
    21841 aaaaaatgcg ccaaaaagaa caggcataaa aagttgttcg caaaaatacc gtctgaaacc
    21901 cgaaaacagg gcttcagacg gcctgaaaag cagcctgaac gcgcaaaaat aaataatgaa
    21961 acggaatgct aagacgcttt caggcagcct gacattaagg ctgcttaaaa gcgtctttta
    22021 atcgtgccac ggattgaaca ccgcgacatc ggccgcaaaa aatgagccgg tatcacgcgt
    22081 agcaactgtc aaactgtgct gttttgcagt ggcggcaata tagccgtctg cggcagcaat
    22141 ctctttgcca tgtgttttgg cataggaacg aatttgcgca tagattgcgg caaccggttc
    22201 atcaaaaggc agaatccgcc ccgcaaataa aggcaaaatg gattgttcca aacgttcgtg
    22261 cagcacattc tttttcttgc cattgagcaa caacgccaca cccaaacgca attctgcaac
    22321 agtaatggca gacaaataca catcttccaa tatcaaacta tccaaccatg ccaccacacg
    22381 ttcattgggt tgtgggcgca aaggttcgga aatcacattc gtgtccagca aaatcatcac
    22441 aaagaaacct cgttatcagt attacgaccg cgtacgtctt ccagctcaac acctccgatt
    22501 tcctgcccta ttgatgccaa cattgacccc aaacgtacag tttgttgtgc tttggcgatg
    22561 ttatccaaaa ttaagcggat ttctgcttcg gtactgcgcc ctgcggctcg cgcacggaat
    22621 ttgattgcgt tgtgcgtggc ctcggataaa tttctaatca caacagaagc cattgccttt
    22681 ccttcctgaa aaaagttttt caaatgctat caaaataaaa aaaatgatag caatctattt
    22741 tttaacgcct gcggatgcct gaaaattcag gtggccgcag gcgttttttt acatctaaaa
    22801 aaacgtctcc cctccctctt tctgataaat aaaaaacagt cgctc
//
LOCUS       DAXDZG010000030        22705 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXDZG010000030 DAXDZG010000000
VERSION     DAXDZG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22705
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.030"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(207..1046)
                     /locus_tag="ACFB78_001009"
     CDS             complement(207..1046)
                     /locus_tag="ACFB78_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC1269021.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1331..1406
                     /locus_tag="ACFB78_001010"
     tRNA            1331..1406
                     /locus_tag="ACFB78_001010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1364..1366,aa:Arg,seq:ccg)
     gene            complement(1471..2664)
                     /locus_tag="ACFB78_001011"
     CDS             complement(1471..2664)
                     /locus_tag="ACFB78_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC1269022.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARTAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2684..3772)
                     /gene="trmA"
                     /locus_tag="ACFB78_001012"
     CDS             complement(2684..3772)
                     /gene="trmA"
                     /locus_tag="ACFB78_001012"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC1269023.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3919..5019
                     /gene="aroC"
                     /locus_tag="ACFB78_001013"
     CDS             3919..5019
                     /gene="aroC"
                     /locus_tag="ACFB78_001013"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC1269024.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5110..5535
                     /locus_tag="ACFB78_001014"
     CDS             5110..5535
                     /locus_tag="ACFB78_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC1269025.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5609..7594)
                     /locus_tag="ACFB78_001015"
     CDS             complement(5609..7594)
                     /locus_tag="ACFB78_001015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC1269026.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSACVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7661..8209)
                     /locus_tag="ACFB78_001016"
     CDS             complement(7661..8209)
                     /locus_tag="ACFB78_001016"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC1269027.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8351..9646
                     /gene="serS"
                     /locus_tag="ACFB78_001017"
     CDS             8351..9646
                     /gene="serS"
                     /locus_tag="ACFB78_001017"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC1269028.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHMLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9719..10717)
                     /locus_tag="ACFB78_001018"
     CDS             complement(9719..10717)
                     /locus_tag="ACFB78_001018"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC1269029.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10842..11918)
                     /gene="prfA"
                     /locus_tag="ACFB78_001019"
     CDS             complement(10842..11918)
                     /gene="prfA"
                     /locus_tag="ACFB78_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC1269030.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12008..12778)
                     /locus_tag="ACFB78_001020"
     CDS             complement(12008..12778)
                     /locus_tag="ACFB78_001020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC1269031.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12781..13773)
                     /locus_tag="ACFB78_001021"
     CDS             complement(12781..13773)
                     /locus_tag="ACFB78_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC1269032.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13840..14523)
                     /locus_tag="ACFB78_001022"
     CDS             complement(13840..14523)
                     /locus_tag="ACFB78_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC1269033.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14713..16047)
                     /gene="glmM"
                     /locus_tag="ACFB78_001023"
     CDS             complement(14713..16047)
                     /gene="glmM"
                     /locus_tag="ACFB78_001023"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC1269034.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLAAVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16177..17031)
                     /gene="folP"
                     /locus_tag="ACFB78_001024"
     CDS             complement(16177..17031)
                     /gene="folP"
                     /locus_tag="ACFB78_001024"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC1269035.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEEWARVEPVLAEAAGWGVPVSLDTR
                     RTVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKY
                     QDVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     LPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKV
                     WEALGVNR"
     gene            complement(17171..18064)
                     /locus_tag="ACFB78_001025"
     CDS             complement(17171..18064)
                     /locus_tag="ACFB78_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC1269036.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18155..20257
                     /locus_tag="ACFB78_001026"
     CDS             18155..20257
                     /locus_tag="ACFB78_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC1269037.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITQPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20321..21799)
                     /gene="ubiD"
                     /locus_tag="ACFB78_001027"
     CDS             complement(20321..21799)
                     /gene="ubiD"
                     /locus_tag="ACFB78_001027"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC1269038.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21819..22334)
                     /locus_tag="ACFB78_001028"
     CDS             complement(21819..22334)
                     /locus_tag="ACFB78_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC1269039.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22341..22705)
                     /locus_tag="ACFB78_001029"
                     /pseudo
     CDS             complement(22341..22705)
                     /locus_tag="ACFB78_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750047.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5049 a         6554 c         6006 g         5096 t
ORIGIN      
        1 tataaaacaa atgggttaaa ccagtatcca tacatcagct tttttatcat cctacttttt
       61 attcatccga tcgggcagac agatttcaaa gatgaaaagc ctattctatt cacacccatt
      121 tgatgtcatt tccacacgga cgaacaaata aattcccata aaaaaacgga gcagatacct
      181 gccccgtttt ttaatttaat ccgaatttag aattttgcac cggattggtt tgccatatag
      241 tcaacagccg ctttgacttc gtcatcgctc aaacctgcat tgccgccttt ggccggcatc
      301 gcgttaaagc cttcaagggc atgtttgtgc aaggtttctt tgcctttttt gatacgcggt
      361 gcccaatcgt cttttttgcc tatgccggga ataccgggaa tcgaaccgcc gtggcacacc
      421 tgacaggttg cttcgaagac ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc
      481 ttgtcttctg ccttcgcttc tgccggagct gcactatcgg caggagcaga agctgttcct
      541 gaagcggcat tgtcggcagg cgcagcctca tcaggattcg ggaaagaacc gccgcttttg
      601 ttcgccatgt aggtaatcgc ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct
      661 ttggcaggca tggcgttaaa gccgttcagc gcgtgttgga acaaggtatc gaagccttgc
      721 gcgatacgcg gcgcccagtc gccgttgtgt tccagtttcg gagcgttcgg cacattgctg
      781 tccgccgcgt ggcattggat acagattttg ccgaaaatct gttcgccttg gcgttcgccg
      841 acggggatgc cgtcacccat cgtcaattgt ccgacaggct ggatgcgggt ctgcgttgcc
      901 gcttccgtag tggcatcgac atcgccgaac gatccactgc ccgccagctt aatcaggaaa
      961 taaaggactg caataacaat aacgataccg ctcacaaggg taaacagtgc agagccttgg
     1021 gctttgttgt cgcggagttg tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg
     1081 tagattatag tttggcgtgt taaacacagt taacaatatt ttgctggatt atactgaatt
     1141 cacagggtct ttccaatcgc tatcattgaa aatatgaaaa aatttgccaa tggtatctgt
     1201 acaaaacaaa taatccgttg aaaataccgg attatctcca agaaaactcc ccttgtgccg
     1261 ccatacgccg cctgccggcg caagataacc tttgccaatt tgcagaattt acgttaacct
     1321 tacgttttcc gcacccatag ctcagttgga agagtgtcag tttccgaagc tggaggtcac
     1381 aggttcgatc cctgttgggt gcgccaatta taaagaggcc gtctgaaaga taaatatttt
     1441 tcagacggcc ttttgactta cttcaaattc ttatttcaac acttcggcaa gcgcatcggc
     1501 gacataagtg atatttgatg tattcagccc ggcgacgcac atcctgccgg aatccagcag
     1561 gtaaacggca aattcgtcgc gcaacctgcg gacttgcccc acgctcaacc ccgtgtagcc
     1621 gaacattccg cgctgtttga tgaaataagt gaaatcgcgg tcggggattc gggcggtcaa
     1681 tacgccgtaa agtttctgcc gcatcgcacg gatgcggtcg cgcatcatat aaacttcgtt
     1741 ttgccacaag gcgtaaagtt cggagctgtt catcacgtcg gcggcgatat acgcgccgtg
     1801 cgcgggcggg ctggagtaga tgcggcggac ggtgaatttg agttgtccga acaccaaatc
     1861 cgcttcttct ttattggggc aaaccacgct taagccgccg acgcgttcgc cgtagagcga
     1921 caggtttttt gaaaacgaat tgctgacaaa cagcggcaaa tccatttcca ccgctttgcg
     1981 gacggcgtag gcatcgctgt ccaaatcgcc gccgaagcct tggtaggcga tgtccataaa
     2041 cggaatcagt ttgcgcgttt tgatgatctg caacacttcg tcccattgcc gttccgacat
     2101 atccacgccg gtcgggttgt ggcagcaggg atggaggatg aggacgctgt tttcgggcag
     2161 ggtgttgaaa aacgcggtca tttcgtcgaa tttcacgccg acagtggcag ggtcgtaata
     2221 tgggtaagtg ccgacctcga aacctgcgcc ttcaaaaatg ccgcgatggt tgtcccaagt
     2281 cggatcgctg acgtaggcgc gcgcttcggg aaaccagcgg tgcaggaaat ccgccccgac
     2341 tttgagcgcg cccgagccgc ccagagtctg tacggtaacg atgcgtccct gcgcgagcgc
     2401 ggggttgcct ttgccgaaca acaaatgctg caccgcgctg cggtaaacgt ccaaaccttc
     2461 catcggcagg tagggagacg gcgcaggcac agcggtgcgg gcggtttcgg cacggcttac
     2521 ggattccaat acgggcattc tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac
     2581 tttttcgggg cgcgggtcgt ttttgaaggt ttcgaccaaa ctcaaaatcg ggtcgccggg
     2641 atagtattcg atgtgtcggt acatagtcct taccttttgc ttttcaaagg attttctttt
     2701 tcaacagtac gccgctttca atatggtgcg taaacgggaa ttggtcgaac agggcggcac
     2761 gtccgaccgt atgggtttcc gtcaaggtgt ccaaattggc gcgcagcgtt tcgggattgc
     2821 aggaaatgta gatgatgttg tcgaactgcg acaccagctt caaagtttcc tcatcgatgc
     2881 cggcacgcgg cggatcgacg aaaatagtgg aaaatgcgta atccgtcaaa acaataccgc
     2941 tctccttaag gcgtgtaaac tcacgttttc cggtataggc ttcggtaaac tcctcagcag
     3001 acaggcgggc gattttgatg ttgcctatcc agtttgcctc aatattccat tgcgccgcac
     3061 tgacggaggt tttggagatt tcggttgcca aaacctgtcg gaaatatcgg gacagcggca
     3121 gggtgaaatt gccgttgccg caatacagtt cgagcaggtc gccgcccaag ccttccgccg
     3181 cgcggcacgc ccattcgagc attttccggc atacagcggc attcggctgg gtaaagctgc
     3241 cttcgatttg gcggtagtgg aaatcgcggt cgccgacgcg cagggtttcc gtcacatagt
     3301 cctgttttaa gactattttc tgccccctgc tccgcccgat aacggaaata tccaactgtt
     3361 gctgtaacgc ttgcgccgcc ttcatccact catcatcaag ccttttgtgg taaatcatgg
     3421 taaccagcat ttccccgctg agcgtggaca gaaattcgac ggcataccaa tggtttctca
     3481 gttcggcaga ttgggcggca acggcaatca attcgggcat caggcagttg actgcctctg
     3541 aagcagcatc gaaacggtcg caacggatca ggcttgcccc gctagccttc tgcccttttt
     3601 caaacatggc gtaaaacatt tcaccgcctt cgtgccaaat gcggaattcg gcgcgcatgc
     3661 ggtagtgttt gtccggagat tcgtacactt cccactcagg aacatccaaa cctgcaaaaa
     3721 gggttttaag gtagtctttt ttaccttgaa gctgctgcgt gtagtcattc atatcgctat
     3781 cctgaaaaat caggccggat tataagccga ttttcctcgc cgcacgcaaa ctctttgact
     3841 gccgcccctt caatgacgga cgggcttttg tgctaaaatc cgccatcttt ccacactata
     3901 ccgataaagg gaaaaatcat ggcaggcaac actttcggac aaatcttcac cgtgaccacc
     3961 ttcggcgaaa gccacggcgc gggtttgggc tgcatcatcg acggctgccc gcccggcttg
     4021 gaattaagcg aagcggatat ccaatttgac ctcgaccggc gcaaacccgg caccagccgc
     4081 cacgttaccc aacgccgcga agccgaccaa gtcgaaatcc tctccggcgt attcgaaggc
     4141 aaaaccaccg gcacacccat cgccctccta atccgcaaca ccgaccagcg cagcaaagac
     4201 tacggcaaca tcgccaccgc cttccgcccc ggacacgccg actataccta ttggcacaaa
     4261 tacggtacgc gcgactaccg gggcggcggc aggagttccg cccgcgaaac cgcagcccgc
     4321 gttgccgccg gagccgttgc caaaaaatgg ctgaaagaaa aattcggcac ggaaatcacc
     4381 gcctacgtta cccaagtcgg cgaaaaaaaa atccggtttg aaggcagcga acacatttcc
     4441 caaaatcctt tttttgccgc caaccaaagc caaatcgccg aactggaaca ctatatggac
     4501 ggtgtgcgca aatccttgga ttccgtcggc gcgaaactgc atatcgaagc cgccaacgtc
     4561 cccgtcggtt tgggcgaacc cgttttcgac cgcctcgatg ccgaaatcgc ctacgcgatg
     4621 atgggcatca acgccgtcaa aggcgtggaa atcggcgcag gttttgacag cgtaacgcaa
     4681 cgcggcagcg aacacggcga cgaactgacc ccgcaaggct tcctgtccaa ccattcgggc
     4741 ggcatcctcg gcggcatcag caccgggcaa gacatctgcg tcaatatcgc catcaaaccc
     4801 accagctcca tcgccacacc gcgccgaagc atcgacattc acggcaaccc cgtcgaactc
     4861 gccacccgcg gcaggcacga tccctgcgtc ggcttgcgcg ccgcgccgat tgccgaagct
     4921 atgctcgcct tggttttaat cgaccacgcc ctgcgccatc gtgcgcaaaa tgcggatgtt
     4981 gccgcggata cgcccgatat ttcccgctcg gacaagtaaa aagatttagc caaaacacgg
     5041 gctttatact aaaataccga ccattgccgt gccgtctgaa cgcacgattc ttagaggaat
     5101 acaaccgaaa tgacacaaga aaccgctttg ggcgcggcac tgaaatccgc cgtccaaact
     5161 atgagcaaaa agaaacagac cgaaatgatc gccgaccaca tctacggcaa atacgatgta
     5221 ttcaaacgct tcaaaccgtt ggcgctcggc atcgatcagg atttgattgc cgctttgccg
     5281 caatacgatt ccgcactgat tgcacgcgtc ctcgccaacc actgccgccg tccgcgctat
     5341 ctgaaagcct tggcgcgcgg cggcaaacgt ttcgatttga acaaccgttt caaaggcgaa
     5401 gttacccccg aagaacaggc gattgcgcaa aaccatcctt tcgtgcaaca ggctttgcaa
     5461 cagcagtccg cccaagctgc cgccgaaacg ccgtctgttg aagccgaagc agccgaatct
     5521 tccgcagcag aataaatccc caaacgaaat gccgtctgaa aaccgatttg gtttcagacg
     5581 gcattttttc gtatgcggca atcacggctc aaatatcgag ttgcgccgta tcgccttcgc
     5641 gttccatcca agcgcggcgg gcggcggctt cgcctttgcc catcagtttg acgaagatgt
     5701 cgcgcgtctc gtcatccgcg ccttcgggga tttgcacctg caacaggcgg cgggtgtcgg
     5761 ggtgcatggt ggtgtctttg agctggtcgg ggttcatctc gcccaagcct ttgaaacggc
     5821 tgatggaata ggcggtttct ttaacgcctt ctttttgcag ccgctctaaa atgccgtcaa
     5881 gttcgttttg gtcgagggcg tagaatttgc gggcgggttt gcttttgcct tgtgcgttga
     5941 catcgacgcg gaacagcggc ggctgggcga cgtagatgtg tccgtcggcg accagtttgg
     6001 ggaagtggcg gtagaacagg gtcaacagca aaacctgaat atgcgaaccg tccacgtcgg
     6061 catcggacaa gatggctatt ttgccgtagc gcaggccgct taagtcggga tggtcgttga
     6121 tggcgtgcgg atcgacaccg atggcgacgg aaatgtcgtg gatttcggcg ttgccgaaaa
     6181 gctggtcggg gtggacttca aagctattga gcactttgcc gcgcaggggc aggatggctt
     6241 gtgtggcttt gtcgcgggcg agtttggcgg agccgccggc ggaatcgcct tcgacgagga
     6301 agagttcgtt ttcgcggatg tcttcgcttt cacagtcggt cagcttaccg ggcaggatgg
     6361 cgacgccgct gccttttttc ttttcgattt ttttaaccga acgtatccgt gcctgtgcct
     6421 gacggatggc gagttcggcg atttttttgc cgaagtccac gttttggttc agccacaatt
     6481 ccaaaggatc gcccgatacg gcggcgacaa gtttcagcgc gtcgcggttg gtcagcttgt
     6541 ctttggtctg accttggaat tgcgggtcga ggacgcaggc ggagaggacg aaggcggttt
     6601 ttccgaacac gtcgtcgctt tgcactttaa cgccgcgcgg taagaggttg tgcaggttga
     6661 tgaaattgtt gacggcgttg aacacggctt gtttcaagcc tgcttcgtgc gttccgccca
     6721 gcgaggtcgg gataaggttg acgtagcttt cgttggcgca cgagccttct tccagccaag
     6781 tcaaagcaaa cgcggcacct tcgccaatgc tgaaatcgcc gttgtggccg tctgaaatgt
     6841 agttttcgca ggagaacagc ggcacggctt cctgtgcgtc ggcaatcagg tcggtcagat
     6901 agcttttcag accgtcgggg taatgccagg tttgggtgtg cgcttcgtct tcgcctttga
     6961 ccggacgggt cagggaaacg cgcacgcccg gcagcagcac ggctttggcg cgcaatagac
     7021 gctcgagttc gggaatgctg taattcgggc tttcaaaata tttgccgtcc ggccacacgc
     7081 gcacttcggt gccgctgtct ttgacggcgc atttgcccac ttgtgccaac ggttcgacca
     7141 catcgccgcc ggcaaacacg atgcggtgga ctttgccgtc tcgtttgaca ctgacttcaa
     7201 gtcgggtgga aagcgcgttg gtaacggata cgcccacacc gtgcaggccg cctgaaaagg
     7261 catacgcact gccgccgtct tttttgttga acttgccgcc cgcgtgcagg cgggtaaaca
     7321 cgagttcgac tacgggtacg ccttcttcag ggtgcaggcc gacgggaatg ccgcgcccgt
     7381 tgtcgcgcac ggaaagcgaa ccgtcttcat gaatttgcac gtcgatttca gtggcgaaac
     7441 cgcccaacgc ctcgtccgcc gcgttgtcga tgacttcttg gcagatatgt gtcgggctgt
     7501 cggtgcgggt gtacatgccg ggacgttctt tgaccggctc caagcctttg aggacggtga
     7561 tgctggattc gctgtattgg ttgtttttag ccataggaat aatctgaaag taagaaaaac
     7621 aacgctttca gacggcctga aagcgttgcg ttccgttatt ttaccggttg ccggaaggtt
     7681 ggcgggcggc aaagtcttca taactttcca taccgcgcag gaagcgggaa gagagttctt
     7741 tcaacgcccc caaataaacg tcgcgtttga aatcaatcac ttcgtcaacg ggcgcccaat
     7801 attgatgcca acgccagccg tcaaattcgg ggtggcgggt ggcgcgcagg ttgacatcgc
     7861 aatcccggcc ggtcaggcgc aggagatacc aaatctgctt ctgcccgcga taagagccgc
     7921 gccattcgcg gcgcacccag ttgttcggca cgtcgtaacg cagccagtcg cgcgtccgcc
     7981 cgacgatttt gacgtgttgc ggcagaagtc cgacttcttc gtaaagttcg cggtacatcg
     8041 cggtttcggg gctttcgccg ggcttgatgc cgccttgagg aaactgccat gaatgttcgc
     8101 gcacgcgctt accccaaaag acttcgttac gttcgttaat taggataata ccgacattgg
     8161 ggcgatagcc ttccctgtcc aacacggtgt cgccctccgt taaattcaat cttgggattt
     8221 tcccacaaat cggacggttt tgacaaatcg gacggtatgg ttcaagcatg tcgaaacacg
     8281 gacggattcg ggaaaatatc ttaaatttgg tttacaataa tacgtttcaa attaattccg
     8341 gaatcagact atgttagata tccaattgct ccgcagcaac accgccgccg ttgccgaacg
     8401 gcttgcacgg cgcggttatg actttgatac cgcacgtttt gacgcactgg aagaacgacg
     8461 caagtccgtt caggtgaaaa ccgaagaact acaagcctcg cgcaacagca tttccaaaca
     8521 aatcggcgca ctgaaaggtc agggcaaaca tgaagaggcg caggcggcca tggatcaagt
     8581 cgcccaaatc aaaaccgatt tggaacaggc tgccgccgat ttggatgccg ttcaaaaaga
     8641 attggacgca tggttgttga gcatacctaa cctgccgcac gaaagcgtac ctcccggtaa
     8701 agacgaaacc gaaaacgtcg aagtccgcaa agtcggtacg ccgcgcgaat ttgattttga
     8761 aatcaaagat catgtcgatt tgggcgagcc tttgggcttg gatttcgaag gcggcgcgaa
     8821 actttccggc gcacgattta ccgtgatgcg cggacaaatc gcccgtctgc accgcgcctt
     8881 ggcgcaattc atgctcgata cgcacatgct gcaacacggc tacaccgagc attacacgcc
     8941 ttatatcgtt gacgatacga cgctgcaagg cacaggtcag ctgcccaaat ttgcagaaga
     9001 tttgttccac gttacccgcg gcggcgatga aaccaaaacc acgcaatatc tgattccgac
     9061 agccgaagtt accctgacca ataccgttgc cggcagcatt atcccgtccg aacaactgcc
     9121 gctgaagctg accgcgcatt cgccctgttt ccgcagtgaa gcgggatcat acggcaaaga
     9181 cacgcgcggc ctaatccgcc agcaccagtt cgacaaagta gaaatggtac aaatcgttca
     9241 tcccgaaaaa tcatacggcg cgctggaaga aatggtcggc catgccgaaa acatcctgaa
     9301 ggctttggaa ctgccctacc gcgtgattac cctgtgtacc ggcgacatgg gtttcagcgc
     9361 ggccaaaacg tatgacttgg aagtttgggt tcccgcacaa aatacctacc gcgaaatctc
     9421 aagctgctcc aactgcgaag atttccaagc acgccgcatg aaggcgcgtt tcaaagacga
     9481 aaacggcaaa aaccgcttgg tacatacctt aaacggctcc ggtttggcag tcggtcgcac
     9541 tttggtcgcg gttttggaaa accatcaaaa cgccgacggc agcatcaata tccccgccgc
     9601 actgcaaccg tatatgggcg gtgttaccaa gttggaagtg aaataagcct taaccctgaa
     9661 cataaatgcc gtctgaaacc tgtttcagac ggcatttcct ttaaactctt aaaacacatc
     9721 aaccgtcggc acgaaccgca ttgccgcaat cgccggtctg tccgacctcg cggatattgg
     9781 acagcgtaac ttccgaaata ttacccaacg cctcttccgt caaaaatgcc tgatgtccgg
     9841 taaacagtac gttatgacaa gacgacaggc ggcggaacac gtcgtcggta atcacatcgt
     9901 tggatttgtc ttcaaaaaac agctcgcgct cgttctcgta cacatccatg cccaatgcac
     9961 cgattttccg gcatttcaac gcctcgatgg cggcggcgct gtcaatcagc ccgccccggc
    10021 tggtgttgat aatcatcacg ccgtctttca ttttgtcgaa cgccgcttcg ttcagcatat
    10081 agtggttttc cggcgtggcg ggacaatgca gcgtgatgat gtccgatcgg gcatacagct
    10141 cgtccaaatc cacatacctg ccgccgagtt tttccacttc ggggttgcaa aacggatcgt
    10201 aagccagcag gttcataccg aaacccttta aaatccgcat cgttgcgata ccgattttcc
    10261 ccgtgccgat aacgcccgcc gttttgccgt acatattgaa gccggtcaga ccttccagcg
    10321 aaaaattcgc atcgcgggta cgctggtagg ctttgtggat acggcggttc aaagtcagca
    10381 tcagaccgac cgtatgttcc gcaaccgatt cgggcgaata ggcaggcacg cgcacgactt
    10441 tcaagcccaa ctcttcagcc gccttcaaat ccacattatt gaagccggca caacgcaacg
    10501 ccacagtttt cacgccaatt tgcgccaatt tttccaacac gggccggctg gcgtcgtcgt
    10561 ttacaaaaat acaggccgct tccgcgcctt ccgccatttt cgccgttttc gcatccagca
    10621 tgaaatcaaa aaactccagc tcgaagccga aatgccggtt ggcgcgggta aaatgttcgc
    10681 ggtcatagct tttcgtaccg taaatcgcaa tcttcatcaa tatgtccagt tgtcgtctat
    10741 ggttgagaaa cggcataata caccgaattc aaacaaatca gtagaatatg gcggattaaa
    10801 attgatttca tgcacggcat ttccatttca aaacacaaaa ctcaatcgcc catcgccgcc
    10861 agaagctccg cctgatgttc ggcaatcagg gcatcggtga tttctgccaa atcgccgtcc
    10921 atcacaaaat ccagcttgtg cagggtgagg ttgatgcggt ggtctgttac cctgccttgg
    10981 ggatagttgt aggtgcggat gcgttcgctg cggtcgccgc tgccgatgag ggatttgcgt
    11041 tcggcggctt ctttggcttg gacttcgcgt ttttgcgcgt cgttcaggcg ggcggcgagg
    11101 actttcatcg cctgcgcttt gttggcgtgt tggctgcggc cgtcttggca ttcgaccacc
    11161 atgcctgtcg gcaagtgggt aatgcggacg gcggagtcgg ttttgttaat gtgctgaccg
    11221 cccgcgccgg atgcgcggaa ggtatcggtg cgcaggtcgg cggggttcag ttcgatgtct
    11281 tcgagctcgt ccgcttcggg catgacggca acggtacagg cggaggtgtg gatgcggcct
    11341 tggctttcgg tggcggggac gcgctgcacg cggtgtccgc ccgattcaaa tttcagacgg
    11401 ctgtacgcgc cgagtccgac aatacgggcg atgacttctt tatagccgcc caattcgctt
    11461 tcgttggcgg acacgatttc aacctgccag cggttgcgtt cggcgtagcg gctgtacata
    11521 cgcagcaaat cgccggcaaa cagcgcggct tcgccgccgc ccgtgccggc gcgtatttcg
    11581 ataaagatgt ttttgtcgtc gtcggcatct ttgggcagca gcagtttttg caattcggta
    11641 tcgagttcgt cgattttggc tttcgccgct tcgatttctt cggcggcaaa gtctttcatt
    11701 tcggggtcgg acaacatttc ttcggcatcc gccaagtcgc tttgggcaag ccgatagttt
    11761 tggaatactt cgacgacggg ggtcagttcg gcgtgttcgc gcgtgagctt gcggtagttg
    11821 tccatatcgg atgtggcttc gggttgccct aaaaggtggg taacttcttc caaccggtcg
    11881 ccgagttgtt gcagtttttc taagatagac ggcttcataa ttcttccata acaaacgccg
    11941 cctgaatttc agacggcatc aaaacgcggt cattataata tattttctga atattcaaaa
    12001 agataatcta gggacggagc agactgtcgg cattgcgttc cgttatcgcg gcgatttccg
    12061 ccgcgccggt accgcgtatt tccgcagcaa tttcggcaat ccgaagaatg ttggcaggcg
    12121 tattgacggt gttttccaac atgaacgggc tatccgtttc caatacgaaa tccccgtcgt
    12181 tcaaggcttt aagcgtatcg cgcactttac gcgcgttcgg attgagcagc agcgaaccga
    12241 tgccgatttt gaaccccagt tttgtcaaca cgcgcgcttc ttccgcgctg cctgagaaag
    12301 cgtgaacgat gccgccttga gtaaagcctg tctgtttgac ggcggcggcg atgtcggcgg
    12361 tggctttgag attatggata atcacgcggc ggcgcagggt ttgcgcgatg gcaagctggc
    12421 ggctaaaaac ttgaatttgc cgttcacgct gcggcggcgt ttgggttttg tcgtaaaaat
    12481 ccaagccgat ttcgccgacc cacgctcggg ggtaacgcac caatacggct tccaaaccgg
    12541 aaaaatcctg ttccgcaatg ccgtctgaaa accaaggatg gatgcccagc gcaatacgga
    12601 tttgaccgtg ttcggacggc atttccgaca gttccgccac gtcctgccaa tcctgcggac
    12661 aggtggcggg aacgataaac cgccacacgc ccgcctgtcg cgcttcggca agcacctgcg
    12721 ccagattgcc gcgcaaaacg gaatcggcaa gatggcaatg tgtgtcggtc agcctcatct
    12781 tcacaccagc cccaaccgct gcaactcgtg tttcaccccg tccgcgcccc atccttcgga
    12841 aacggcaagc gtcatcaatg cggttgcggt ttccaaattg catttgccgc cgttgaccac
    12901 gcccgcccga cgcagcgcgt cgccctgtgc gtaaacggcg gcagcgcaac cctgcggcac
    12961 ttggctgatg ttaagcaaca atttgccttg ctgcgtaaag tcttgcacgg cgcggatgaa
    13021 gccttcgccg gcggaggtat tgccgtgtcc gtagctttgc aggataaggg cttgggcggg
    13081 aagctgtccg agcccgtcgg caagttcttg gacggcaaag ccggggataa gcgtgcggac
    13141 ggcgattttt gcctgcgggt cgggataacg gatttcgagg ccgtctgaaa catctgccgc
    13201 gtcctgattc gggatgcgga cattgtgcca gccccgggtt tcgtcccact cggcaagtgc
    13261 gccgaaatgg gcattgccga aaccggcggc ggtttccgtg ctgattttgc tgctgccgac
    13321 ggcgggatac agtttgccgt caaaggcaat aagcgtctgc ttcaatccga gtgggaatgc
    13381 ggcaacggca gtcaccagat tgcgcggagc gtcgctgccc tctgcgttgt acggccattg
    13441 cgaaccggtc aggacgatcg gtttgtccaa accttgcagg gcgagggcga acatattggc
    13501 ggtataggcg agtgtgtccg tgccgtgcag caccaaaaca ccgtcgtact gcggcagcgc
    13561 ggcggcaatg atgtccagcc aagcgcacca gttttgcagt tggacggcgg aagaatcaat
    13621 cagcggctca caaacgtgcc aatcgaaatc catgccgtct gaaaacggcg cgagtgcctg
    13681 cccgaccagc gcggtatcgg ggcgcaagcc cgcactgctt tgcgtcatgc cgatggtgcc
    13741 gccggtataa aggacaaaaa ttttctgttt catggctcat tctgaaaatg caaagaccgc
    13801 ctgaaacgcg aaatgcgtcc ggacggtcga aaatccgatt tactgctgct tctgtgccgc
    13861 ttttttcgct gccttttccg ccttgcgttt ggcgcgtttt tcgctcaatt gtgcgcggta
    13921 aagctgataa tgtcggcgtt tgcgccacca gaaccacgcc agcgccgccg ccagcacgcc
    13981 caatgcgatg aagatgcccg attgcaggct gtgcattttc gccatcagcc aatcgatgtt
    14041 gtgcgcgccg tactcgccca agtaaatcca aacgggcacg gaaatcagcg cggccagccc
    14101 gtccataatc agaaagcgca gatacgatac tttgcggctg atgccggcgg taacgaaaac
    14161 ggcagtccgc aaacccggca ggaaacgggc gacaaacaga acccagttgc cgtatttgtc
    14221 gaatttttcc tgaacctgcg cgtaacgttt cggcgtcatg atgcgcgcaa tcggtttgaa
    14281 cttgaggatt ttctgccccc agatgcgtcc ggcggcaaac atcacgccgt cgcccgccaa
    14341 cacgccgagc ataccgaccg caaacataat atgcggattg gtataaccca tacccgaaat
    14401 cacgccgccc gttaccaagg tcaaatcttc gggaatcggc acgccgaaac cgcagatgac
    14461 caaaacgaaa aacacggccg catagccgta ttcgacaaaa aaggcttcca aaagggcaaa
    14521 catagcggat attccattgt cggagataaa aagtcagaac aagccgaaac attttctgcg
    14581 cgaaacggca ttctatcaaa gattatgccg tctgaaagcg gcaggggggg cagattcgcg
    14641 cccccgccgg acgttcagac ggcatttaaa caggaaaacc gccgctgttg aaacggtttt
    14701 gcctgatttt gcctaaatgc cgccgatggc ggcggcgata cgctccgccc cgtcgcgcgc
    14761 ccaatcggcc tgacgcgcct cgaccatcac gcgcacgacg ggttcggttc ccgaagcgcg
    14821 caacacgaca cgccctttgc cttcgagttc tttttccact tcggccaaca cgtctttcga
    14881 agcctcctgc catttctgac ccttttggat gcgcacgtta atcatcgttt gcggatacgg
    14941 ctgccagtcg gcgcaaacgg cggcgaggtc ttggttcagg atttgcagcg ccgccaagac
    15001 ttgcagcgcg gaaataatgc cgtcgccggt gttatgtttg tccatgcaca aaatatggcc
    15061 gctggcttcg ccgccgatga gccagccgcg ttggttcagc tgttccaaca catagcggtc
    15121 gccgactttg gcgcggcaga aatcgacgcc ctgctctttc agggcgattt ccatcgccat
    15181 attggtcatg accgttccga ccacgccgcc gatgttgata ccttctcggg cgcgggcttt
    15241 ggcgatgacg taaatcaggc tgtcgccgtc gtaaacctgc ctgtttttat cgaccatcat
    15301 caggcggtcg ccatcgccgt ccaaggcaat accgtaatcg gcttcgtttt gcaacacggc
    15361 ggcttggagg gttttggtat gggtcgcgcc gcatttttcg ttgatgttgt agccgttggg
    15421 ttcgttgccg atgctgacga cctgtgcgcc gagttcgtgg aacactttgg gtgcaacgcc
    15481 gtatcctgca ccgttggcgg tatcgataac caacttcaaa ccccgaaggt cggaatggcc
    15541 ggggaaggtg gatttgcaaa actcgatata gcggtcgtcc gcaccgctga tgcggcgggc
    15601 gcgtccgaga cgtgcggacg gttgggtttt catttcttca tcgattttgg cttcgatttc
    15661 caactcgact tcatcggaaa gtttcacgcc gccttcggca aagaatttga tgccgttgtc
    15721 ggaataggtg ttgtgcgacg cggaaatcat cacgccggcg gacaggcgca atgcgcgggt
    15781 cagataagcc acaccgggcg tgggcaacgg cccggtctgt accacattca cacccgccgc
    15841 cgtaaagccc gctaccagcg cggcttccag catatagcct gaaatgcgcg tgtctttgcc
    15901 gatgaggacg gtcggtttct gatcggtatc gtgctgcacc agcacctgcc ccgccgcata
    15961 gccgagtttc aatacaaaat cgggcgtaat cggaaactga ccgacttcgc cgcgcacgcc
    16021 gtccgtgccg aaatattttt ttgccatcat ttgctccgtg aatgtgaacc gttgtccgag
    16081 attatacagt cagtttgcgc cctgctgcct gcaccgttga tgccgtctga agccgtcccg
    16141 cccttttcag acggcattcg gcgcaaaaac cgccgtttac cggttcacgc ccaacgcttc
    16201 ccacaccttc aacgcgtccg ccgtcgcctt cacatcgtgc acgcgcacga tttgcgctcc
    16261 gcgtgccacg gaagccaaag ccgctgccac gctgccgtgt acgcgcgccg ccgcgtccgc
    16321 ctcaccggtc agctcaccta tcatgctttt gcgcgacaca ccgatcagca gcggcaaacc
    16381 ggtttccgcc atcaattcgg gcaaatgccg catcagtgcg atattgtgtt gcaggttctt
    16441 gccgaagccg aaacccgggt cgagcgtaat ccgttgcggc gcgatgcctg ccgcgacaca
    16501 ggtttctgac cgtgttttca gataacgtgc cacttcgccg accacatcct gatatttcgg
    16561 attgtcctgc atggtttcgg gcaagccccg catatgcatc aggcaaatgc ccgtgtccgc
    16621 ctggcgcgcc agcaattcga ccgcaccttc gtcggtcaac gccgccacat cattgataat
    16681 atcgatgccg ccgagtgcca acgccttttc cataaccacc gtgcggcgcg tgtccaaact
    16741 gacgggaaca ccccaccccg ccgcttccgc caatacaggc tcaacccgcg cccattcttc
    16801 ttcttcgggc ggaacgaaat ccgcacccgg ccgcgtcgat tcgccgccga tgtcgagaat
    16861 gtccgcaccc tctttcaaaa gccgttcggc atgcgccaaa gctgtttggg cgttttgcga
    16921 atacgcgccg ccgtcggaaa aagaatcggg cgtgagattc acgatgccca tgattttcgg
    16981 tttgtccaaa ccgatttcaa accgtcctgc ccgccaaacg tgtcgtgcca tctgaactcc
    17041 tcccgaaata aaaaacagat tatatgccgt ctgaaaccgt cttgtgcgct tcggacggca
    17101 ccgctattcg ggcggcagac ggcatgttgt cccaatgtct gcgccgcctt tgaatctgcc
    17161 ggcatgcctg ctatccgccc gccttttcaa aacaggttcc gacgattccg cacgcgcctg
    17221 ccgcctttgc caagccgtac aggatttcct gcggcatatc gcggctccat aatcccgtaa
    17281 tattcgcaat cacgggcaga tggctgattt ggcggacttt cacgatggat tcgacatcca
    17341 aacggtaggg atggcctttg gtatggccca acacgcccga ctcgcccaga atcaggtggc
    17401 ggttgccgcg cgagacgaca tattctgcgg cattcaacca atcttcggca gaatggtgct
    17461 tgtctttaca cagaaccaag ggaatgttca gacttccggc ttcattcaat acggcaagat
    17521 cggacatcag cccgccgccc aaatacagga tgtccgctcc cgcattcaaa gccgcttcga
    17581 catggcggac gttgcgaacg cgcaccaata cgggtttccc tgcgccatgc gccgatgcgg
    17641 cctgttccgc caaccgtctg caccgtcccc gcccttcatc cgcacttaaa gtgtcgtata
    17701 agtttgccga agtgaaaaac ggatccagaa acactgcatc cgcattgctc catactgacg
    17761 gttctgcagc gatacggacg gtttccccgc cgccgaaagc cacgcctttg gcggcaacgc
    17821 ggctgtcttc cgcccgattt tcccgactga cggttttcca tgtgtccaaa atgcggacgg
    17881 ctttctcgac ctccggcagc gtctgtacct ccctgacgct caaaacccta tcgtcgccga
    17941 ttgcgccgat gacggtacgc tcgtcgccgt gagaaatgtg ttctcgcaga cctctgctgc
    18001 ggatgaaggc gacaacgccg gcaatgtccg cttcggcggc acgcctgctc atgacaataa
    18061 tcatatttcc tcctgacaca agagacggcc cgcccaaaat aggatttttg caagccgtgt
    18121 tatactgtgg cgtgttttac agattgttcg ggctatggat ttattatcgg ttttccacaa
    18181 ataccgtctg aaatatgcgg tggcggtgct gacgatgctg cttttggcgg cagtcgggct
    18241 gcacgcttcc gtatatcgca ccttcacgcc cgaaaacatc cgcagccgcc tccaacaaag
    18301 cattgcccat acccaccgga aaatctcgtt tgatgcggat atacggcgca ggcttctgcc
    18361 ccgcccgacc gtcatcctga aaaacctgac cattacccaa cccgacggcg gccgggtcgc
    18421 cgtttccgtc aaagaaacca aaatcggatt gagctggaaa aacctgtggt cggatcggat
    18481 acaggttgaa aaatgggtgg tttcgggtgc ggatcttgcc ctgacgcgcg acagaaacgg
    18541 cgcttggaac atccaagacc tgttcgacgg cgcgaaacac tccgcctcag tcaaccgcat
    18601 tatcgtcgaa aacagcaccg tccgcctcaa tttcctgcag caacagctta tcctgaagga
    18661 aatcagcctc aacctgcaat cccccgattc gtcggggcag cagtttgaaa gttcgggcat
    18721 actggtttgg agaaagctgt ccgtcccgtg gaaaagcagg gggctgttcc tttcagacgg
    18781 catcggcacg cccgaaatct caccgttcca ttttgaagct tccacttcgc tggacggaca
    18841 cggcatcacc atttccacca ccggcagccc ttctgtccgc ttcaacgccg gcggagcgga
    18901 tgccgccggc ctcggcctgc gtgcagacac ttccttccgc aacctccacc tgaccgcgca
    18961 aatccccgca ctggcactca aaaacaacag catcaaaacc ggcacggtca acggcacgtt
    19021 taccgccggc ggcgaatatg cccgatggga cggttcgttc aaactcgaca aagccaacct
    19081 gcactccggc atcgccaaca tcggcaacgc cgaaatctcc ggcagcttca aaacaccgcg
    19141 ccttcaaacc aatttctccc tcggctcgcc gttggtttgg agtcgggaca acgggctgga
    19201 cgccccgcgc ctgcacatat cgacccttca ggataccgtc gaccgcctgc cgcaaccccg
    19261 tttcatcagc cggctcgacg gttcgctgtc cataccgaat ctgcaaaatt ggaatgccga
    19321 attaaacggc acattcgacc gccaacccgt tgccgcaaaa ttcaaatata cgcgggaagg
    19381 cgcaccgcac ctggaagccg ccgccgcgct gcaaaaatta aacctcgccc cctatcttga
    19441 cgaatttcgg caacaaaacg gcaaaatatt ccccgacatc ctcggcaggc tgtccggcaa
    19501 cgtcgaggca cacctcaaaa tcggcagcat ccaactcccc ggcttgcaac tggacgatat
    19561 ggaaacctac ctccacgccg acaaagacca tatcgcgctc agccgtttca agtcagggct
    19621 ttacggcggc cataccgaag gcggcatcag catcgccaac acccgtcccg ccacttaccg
    19681 cctgcaacag aatgcaagca acatccaaat ccaaccgctg ctgcaagacc tgttcggctt
    19741 ccacagcttc agcggcaacg gcgatgcggt catcgacctg accgcaagcg gcgaaaaccg
    19801 caaacagctt atccgctcgc tgcaaggcag cctgtcgctg aatatttcca acggcgcgtg
    19861 gcacggcatc gatatggaca gcattttaaa aaacggcctt tccggaaaaa tctcgggcag
    19921 cacacccttc taccgattca cgctcaacag cgaaatttca gacggcatca gccgccacat
    19981 cgataccgaa ctcttctccg acagcctcta tgttaccagc aacggctata ccaatctgga
    20041 tacgcaggaa ttgtctgaag atgtccttat ccgcaacgcc gtccatccga aaaacaaacc
    20101 gattcccctg aaaatcaccg gtacggtgga caagccgtcc attaccgtcg attacggcag
    20161 gctgaccggc ggcatcaatt cgcgcaaaga gaaacagaaa atcctcgaag acaccctgct
    20221 ggaacaatgg cagtggctca aacctaaaga accgtaaata ccctgcgtac aaaaatgccg
    20281 tctgaaacac attaatcttc agacggcatg accgtaaaac ctacaacccc aatttccccc
    20341 aaatcccatc aattttaacc gtaaccgcag ggtctttttt gattacccgt ccccattcgc
    20401 ggtcggtttc tcccggccat ttgctggtcg catccaaacc cattttgccg ccgagtccgc
    20461 tgacggggct ggcgaagtcg aggtagtcga tgggcgtgtt ttccaccaaa acggtgtcgc
    20521 ggacggggtc catgcgcgtg gttaccgccc agatgacttc tttccagtcg cgcacgttta
    20581 catcgtcatc caccacgatg atgaatttgg tgtacataaa ctggcgcagg aacgaccagc
    20641 agcccgtcat cacgcgcttg gcgtgtccgg cgtactgttt tttcatgctg accaccgcca
    20701 tgcggtagga acagccttcg ggcggcaggt agaaatcggt gatttcggag aactgctttt
    20761 gcaaaagcgg tacgaacact tcgttcaacg ccacgcccaa aacggcaggt tcgtcgggcg
    20821 gtttgcctgt gtaggtagag tggtaaatcg ggttttcgcg catggtgatg cgttcgaccg
    20881 taaacacagg gaaatggccc tgctcgttgt aatagcccgt gtggtcgccg tatgggcctt
    20941 ccaacgcggt ttcgtttgga tgaatcacgc cttccaatac aatttcagca cgggcaggca
    21001 cttgcaaatc gctgccgata catttcacca gctccgtccg cgaaccgcgc agcagtccgg
    21061 caaactggta ttcgctcaaa gtatcgggaa cgggcgttac cgcgcccaaa atggtggagg
    21121 ggtcgcaacc gagtacgacg gcgacgggat acggcgtatc gggattgagt ttgcggaatt
    21181 cctgataatc caacgcgccg ccgcgatgcg acagccagcg cataaccagc ttgtttttgc
    21241 cgatgagttg ttggcggtaa atgccgagat tttggcgttt tttgtgcggc ccgcgcgtga
    21301 cggtcaagcc ccacgttacc agcggcgcaa cgtcttccgg ccagcaatgt tgaatcggaa
    21361 gctgatacaa atcaacgtct tctccttccc acacgatttc ctgacacggc gcgtttttca
    21421 ccacgttcgg cgccatgctc caaatatctt tcaacagcgg cagtttggaa aacgcgtctt
    21481 tgatgccttt gggcggttcg ggttctttca aatacgccag cgtctgcccg atttcgcgca
    21541 gcttggacac gctgtccgcg cccatgccca tcgccacacg ttcgggggtg ccgaacaggt
    21601 tcgccaacac gggataatca tagcgcgtac cgtcgggctt aatcgggttt tcaaacaaca
    21661 acgccggccc ttcggcgcgc aacacgcggt cggcaatttc ggtcatttcc aaatgcgggg
    21721 aaacggggtg ggcgacgcgc ttgagcttgc cctgctgctc gagcatagcg atgaagtcgc
    21781 gcaggtcttt gtatttcatg tttatccttt ttgtcctttt atcctgaaca atccgattcg
    21841 gataccgccc ctatccttgc ctgcgcttcg gcatatccca tgccgtgata aaagtcgcgt
    21901 accggcggat gtccgccgct ttgatgaagt tgcagcaaag ggcgttggtt gtcggactgg
    21961 gtaacgacat tgcagtgcag accgaaggtg tcggtttcat aagggggcag ccgattgcaa
    22021 atcataccga aataaacggc gttttcaggg ttgtcgtaaa agcggctttg atagtcgtta
    22081 aaactctttt cgctgacgga cacccacacg ccgtattcca gcgtttcctg atgcccgata
    22141 atcggaatag gcagcgccgc gcggataaag cggtcggttt ggccggaata gcggatgatg
    22201 cagaaatcag aatcgcattc cgcttgataa gcaatgcgtt cttcttcact gagttgatta
    22261 tagggatagg gggcggtaaa gccggttgcg ggcatatctt catgtttttc gccgcaggaa
    22321 gtgcaagtgt gcataaggtt tcagactttc aacacaagtt tgcggtaaag ccattcgccg
    22381 gcaaacagca tccccatcaa tacataggca atcacgcctg tataaaccgc ccaccaatca
    22441 tatcgcccca accgggccaa caaagcggca agcgtcccgt tggcaataaa gaatacgcac
    22501 caaacctgcg ttacccggcg ggtatagcgc acggcttttt caggcaggtc gggctgttgc
    22561 agccgcgcaa gtttttcaat caccgtctgc ccggcaaaca ggctgctgcc gaacaccgcc
    22621 aacatcatca aattaacgag gacaggatac caatacattg aatcacgccg cccgaacacc
    22681 aactactgcg gcaaaaaaca gtgta
//
LOCUS       DAXDZG010000031        22632 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXDZG010000031 DAXDZG010000000
VERSION     DAXDZG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22632
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.031"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            149..277
                     /locus_tag="ACFB78_001030"
                     /pseudo
     CDS             149..277
                     /locus_tag="ACFB78_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            490..1827
                     /gene="ribBA"
                     /locus_tag="ACFB78_001031"
     CDS             490..1827
                     /gene="ribBA"
                     /locus_tag="ACFB78_001031"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC1269040.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1990..2106)
                     /locus_tag="ACFB78_001032"
     CDS             complement(1990..2106)
                     /locus_tag="ACFB78_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC1269041.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(2138..3739)
                     /locus_tag="ACFB78_001033"
     CDS             complement(2138..3739)
                     /locus_tag="ACFB78_001033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC1269042.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(3806..4645)
                     /locus_tag="ACFB78_001034"
     CDS             complement(3806..4645)
                     /locus_tag="ACFB78_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269043.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANSV
                     QPI"
     gene            complement(4656..5216)
                     /locus_tag="ACFB78_001035"
     CDS             complement(4656..5216)
                     /locus_tag="ACFB78_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269044.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            complement(5209..6411)
                     /locus_tag="ACFB78_001036"
     CDS             complement(5209..6411)
                     /locus_tag="ACFB78_001036"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1269045.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            complement(6461..6691)
                     /locus_tag="ACFB78_001037"
                     /pseudo
     CDS             complement(6461..6691)
                     /locus_tag="ACFB78_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(6740..9744)
                     /locus_tag="ACFB78_001038"
                     /pseudo
     CDS             complement(6740..9744)
                     /locus_tag="ACFB78_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(9864..11174)
                     /locus_tag="ACFB78_001039"
     CDS             complement(9864..11174)
                     /locus_tag="ACFB78_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC1269046.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            11532..14726
                     /locus_tag="ACFB78_001040"
                     /pseudo
     CDS             11532..14726
                     /locus_tag="ACFB78_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            15039..15233
                     /locus_tag="ACFB78_001041"
                     /pseudo
     CDS             15039..15233
                     /locus_tag="ACFB78_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(15254..16170)
                     /locus_tag="ACFB78_001042"
                     /pseudo
     CDS             complement(15254..16170)
                     /locus_tag="ACFB78_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            16425..17889
                     /locus_tag="ACFB78_001043"
                     /pseudo
     CDS             16425..17889
                     /locus_tag="ACFB78_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            18140..18508
                     /locus_tag="ACFB78_001044"
     CDS             18140..18508
                     /locus_tag="ACFB78_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269047.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGRIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            18581..19990
                     /gene="thrC"
                     /locus_tag="ACFB78_001045"
     CDS             18581..19990
                     /gene="thrC"
                     /locus_tag="ACFB78_001045"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC1269048.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            20037..20831
                     /locus_tag="ACFB78_001046"
     CDS             20037..20831
                     /locus_tag="ACFB78_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269049.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            21019..21795
                     /locus_tag="ACFB78_001047"
     CDS             21019..21795
                     /locus_tag="ACFB78_001047"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC1269050.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
BASE COUNT         5924 a         5284 c         5433 g         5991 t
ORIGIN      
        1 gaagattcga agcggttgcc ggcttcctgc cgtccgccgg cgcctccgtc atcacgccgg
       61 caaccgcctt tttcatccct tccttatctt ccatggtacg aatcctcaaa aacgggcaaa
      121 aaaaagccct gttacttgta gaaagtaaag ggcaatccgt ccggttttta tcgggaaggc
      181 ggcattcatc ataggattgc cggtattgcg cctgctcgta ccgcctatgc cgtacccggc
      241 aatcaaggct ttttatcaga attttttttc ggaataaccc tccggaaacc ccataatcag
      301 aagcgtgcgg gaagaaacag ccgccccgcc ggcggggttg cggcaatgcc gtctgaagcc
      361 acgaatccgg cttcaaacgg catctgttta ccaaaaggca aataatccga tttggcgaaa
      421 aacaaatttg ctttttagta aatacgcgct tacaatccgc tacatccgat ttctacaaag
      481 gataaaacga tgaccgacac agccggcttg cgccgccgca acctgcggca atggatagcg
      541 gaacactacg gcggactgca aaaccgtttt gccgaagccg ttgccctcaa cacaggcgaa
      601 ctctccgccc ttttgaaaaa caaatccttc ggcgagaaaa aagcccgcaa aatcgaacag
      661 gcggcaaaaa tgcccgcctt ctggctcgat accgaacaca ccggccgacc gcccggacac
      721 acaggaaaac acaccatgtc ccatatttcc cccatccccg aaatcctagc cgacatcaaa
      781 gccggtaaaa tggtcatcat caccgatgcc gaagaccgcg aaaacgaagg cgacctgctg
      841 atggcagcgc aattcgttac ccccgaagcc gtcaacttta tgatcaaaca cgcgcgcggc
      901 ttagtctgcc tgcctatgga aggtgcaatg gtcgaaaaac tcggtctgcc gatgatgacc
      961 caaaaaaacg gcgcgcaata cggtaccaac tttaccgttt ccatcgaagc cgcgcacggc
     1021 attaccaccg gcatttccgc cgccgaccgc gccctgacta ttcaaaccgc cgtttccccg
     1081 accgccaaac ccgcagacat cgtccaaccc ggtcatatct tcccgcttcg cgcccaaaaa
     1141 ggcggcgtac tcgtccgtgc cggacacacc gaagccggcg tcgacttggc gcaaatgaac
     1201 gggctgattc ccgccgccgt catctgcgaa atcatcaacg acgacggcac gatggcgcgt
     1261 atgcccgaac tgatgaaatt cgccgaagaa cacaagctca aaatcggcac gattgccgac
     1321 ctcatcgaat accgcagccg caccgaaagc ctgctcgaag atatgggcga cgcgcccgta
     1381 caaactccgt ggggcgagtt ccaacaacac gtttacgtcg acaaactctc cggcgaaacc
     1441 cacctcgccc tcgtcaaagg cacaccctcc gccgacaccg aaaccctcgt ccgcgtccac
     1501 gaacccttca gcgtgatgga cttcatccaa gccaatccgc gccattcctg gtcgctgccc
     1561 aaagcccttg agcgcgtcca acaggccgaa agcggcgtcg tcatcctcct gcaccacacc
     1621 gaagacggcg catccctgct cgaccgcacc ctacccaaag gcgcaaacca agcctacaaa
     1681 tgggacagca aaagctacgg catcggcgcg caaatcctcg ccggcctcca cgtcaaaaaa
     1741 ctgcgcgtcc tcggccaacc gtcatctttc accggcctca ccggcttcgg cctcgaagtc
     1801 gtcggctttg aagaagcgga aaaataatat agtaaattca aatgctttat atttgcttca
     1861 tttattgcat tatttccgta caaacgaaaa cccggtctgt tgggttggat tttgcttttt
     1921 tcggatttcg ggtaacttct aattcgtcat tcccgcgcag gcgggaatcc ggttcgtcgg
     1981 gtttttgtgt catttccgat gaattcctgt ggctttggtt ttttggattc tctctttcgg
     2041 ggaaagaacg gcataagtat tttccaaacc aaacaaaatg ccgtctgaaa ggctttcaga
     2101 cggcatttta agtttgaccg gtttcattca tcggtattta tgaattgaat ttcaacatcg
     2161 ccaatctatc cttaatctcc ttttccaatt cggcagattc ggcgaaaagt ttatccaaat
     2221 ccgctgaaaa tcccgctatt ttttgcacaa attcgtcggc ggatatatcc acataatcaa
     2281 tttttacctc aaaatactgc cccgccgaca agctgtgatt cttcgctttg atttcatcat
     2341 agccgaccac cacgctgaaa tcttctactg cctgtttgtg cgtgaaggta tggcagattt
     2401 tttgttcttc ttcgtgggaa agtacagttt tttggttgtc attaatacta attttttcac
     2461 ctaaaccgga agcatcgatt aatacaactt tatccttatt gactttatca ataaacaaga
     2521 tagaaacatt ggttcccgtc gtagcgaaaa tattggaagg catagacacc acgccggcga
     2581 gcattttgtt ttccaccaga tattcgcgaa tttttttatc gatgcccgat tttgccgtga
     2641 taaagccggt cggtaataca atcgctgcct tgccgttttc tttcaggctg aacagaatat
     2701 gctggataaa aagctgatag atttccatct tctctttctt tgttggcttg attttcggga
     2761 taccagcaaa aaagcgttcg tggttttcat cgctttccag ccgatcgcgg aaatcgctga
     2821 aatccaattt aaacggcgga ttggaaacaa tgaaatcaaa ttttttcaga cagcctgaag
     2881 cgtctttgtg ggcgggcgac aaaatcgtat tgccctgaac cacattattc agcgaatgca
     2941 ccagattgtt caaactcaga ttcagccgca acaaattgga cgatttctgc gaaatatcct
     3001 gagtataaat catgcattta tcttcgccga tgacatgggc gacattcatc aacagcgttc
     3061 ccgaaccggc cgacgggtcg taaacgtcca cgctgcggat ttgcccgcgc acctcctccg
     3121 gcaccaaaat atccgccata atccgcgcaa ccgcgtgcgg cgtgtagtat tcggcatatt
     3181 tgccgccgga attactgttg tagtctttaa tcaggtattc aaaaatcgtg gcgaaaaagt
     3241 cgaatttttg cgcaaaaatc gcctcgaagc tgaaacccgc cagcttggaa atcagcgcgc
     3301 ggcaaaaatc gtcgcgtcgg ccttcgtcgg taatgtactg actgatacgc tcgaacaaca
     3361 cgattttcgc cccgccttcg gttttcaccg aaaacaaatc cgcattacag gcggcaatat
     3421 cggtgagcgt atcgtcaaag agtttggcaa aatccgcgcc gttttgccgc tctgataaat
     3481 acttgatgga atgttcgggc tttaaaaccg ctgtttttcc atcaatatcc atattgacga
     3541 actcgatcgg ctcgtcgggc ttttctttgc gaattttttt aacttcaaaa tcgtatttgt
     3601 cgtttaggaa tttatacaaa aacgcctgac tgatgatttt aaattcgttg ccgtcattgc
     3661 ccaaaccata gttggcgcag atggttttta agctgtcgat tagggatttg atttgttcgg
     3721 taaagtgttg ttcggtcatg gaatgtctcg gtttgttgct agcgtcgctt aaattttgtt
     3781 tagcattagt tggtttgctg gcagattaaa taggttgaac actatttgcc aaccttttct
     3841 cttttcctaa tgctgttgtg taataacgtt catcaatacc atataacaaa tgtttcagct
     3901 gtacatcagt agaaatttct acacgagagc catctgcgtt aaatttcaag atattattat
     3961 attcagcaag gtaatttaat agttcttgtc tttgatctag cgtcatatta ttcaatctat
     4021 cttgaactaa agctattctt ttcctattgg gaatagatac tttttcactt aaaaaatctt
     4081 cttttaaatc aataaaatcg ctttctaaga attgctgtac ttcagtattt gtagcttctc
     4141 gatacaaatc ttcaatccct ttaaatatat ttgaaataga agaaagggtt ctaaatatta
     4201 atttatcatc ctttattata taaacagcat caggaatatc tttaataacc aataagtctt
     4261 gtggttcgca tagtttcgca ccatttccgc atatagttag caagtgcttc tttttgagta
     4321 ataatgaact tgtgattttc tggaagaata gtgcatcatt agtacaagat actaaatact
     4381 ttagtttctc tatatttatt tcttcctttt ttatttcact aaacattttt gaatcaaata
     4441 aatttgagtt atccaaaaac ttaggacaaa aatcttgatt ctttaaattt tcaatttgaa
     4501 accaagtatc atcatcaaga ttgtaatcat agttatatgt tataaaattt aaattctcgt
     4561 aagaaaagtc agggaaaata tgatcatcag ataatatttt tactaaatct ttcttgcaag
     4621 taattttagc taaaacaaaa ttcattattg aactcctatt tttctaagaa agtaaaatta
     4681 ttaattcgat atacagcaat cgaacttaca ctttgtggcg ttctaaattc ctgtttacta
     4741 atcagcacta ataataaccc tgattcagtt tttgcttgat aaaatcgata gccaaataag
     4801 aaaaaaacag ggttaaaata aaatgactta gagcatgaaa ctagtaagaa aataattcca
     4861 tacataacaa atagcaaaaa aagattatta tcgggtatgc ttagagcaac aaaaaaataa
     4921 ccaagatagc taggtaagaa attatcgtta acatattcta attctttcac ctctccttgc
     4981 ttgaattcat ccttccccaa gtatttgctt agccataaga cagtagcggt tgcgataaat
     5041 ggtattagaa aataaaaaag tagacttaac ttagaaatag gttctatcca atgatttgaa
     5101 attacacgat aaaaccaagt aaggtctaat atataatatt gcgatttaat aaaatatatt
     5161 gataacagca aagatgtact acttatattt aataatattt taaatagttt atccatctct
     5221 tgctccgcta catcttacgg atacctgccc gttcatcagc atgggtagta ggaaatcgcg
     5281 tagttgggtc aggtggtggt tttgttttag attatttcga actttaacaa agaaagtatt
     5341 tgctatttca ttatattttt gagaaatatc tttactaggc aaaattattt taaattcttt
     5401 taagaattta aaatgtctag cataaccata acttgataaa tctatttgat ttatgatttg
     5461 gtaaaacaaa tagtttggca ttcgttcatt attgcttaat attacttgtg taccatctgc
     5521 tccccgcgca tattgaaaat taactaactt aacaattctt gtatgatcac caaaaataat
     5581 atgagcatct tgtgggttta atatagattt ttcatcattg gtaaatccac aaataaaatc
     5641 ttgactttgg tcaacaacag gatattttcc aaaatcctta atatctttat ttaatatttt
     5701 tgtagtattt gggatttttg ctaagcaaga ttgtaattct atacttcccc accccttcgg
     5761 aatttcccgt ttcaaggttt cgtcaaacac catgtcgccg cctgaggatt tgtagggctt
     5821 gccgttggcg tcggggaaat cgaactgcac aaaccagtag tcgtacaggg ttttcgccat
     5881 ctcttccagg cgcgcgttga tttgtttgtt cagggcgatt tttttgtcca aagcggatag
     5941 gacggcggcg atggattgtt ggatttgagg ttctggaata ggcaactcca gtgtttttaa
     6001 tgcattttca ttgacacgtt gcctgccgga agttccttcc atacattcaa tcgctcgttt
     6061 tctaaaatcg ggactgattg caaaataata gagaaattcg ggattggttt cattttttgc
     6121 tcttaacacg ataaattcgg tagaaccgaa agccacttct ccatcatcta aaatatctac
     6181 aaaagcggtt ttcccatttt ccaaacacgg ggtaatctta gctaataagg tatcaccatt
     6241 gcgaaactta gctccaccgt taaatgcctt gatttcgtaa cccgtaattt gtcgttgaaa
     6301 ttctttcaac attgccatcg gaacactttt tgccaatgct cccttggcta gttgctctct
     6361 cggattaaaa tcagcaatat tttgaatttg ttgtttctta aaatgattca tgatgttccg
     6421 ttacaaaact cttcaaaggc cgtctgaaaa aaacatttca tcggtagttc ttgccgtctg
     6481 cggcagttgt aaactgaaag tttacaactg aatcgtccag ttcttgttct gcaaatatgg
     6541 ctttgatgtg tttaccgatg ttttgtttgc tggtttggta gagttcggca atttcttttt
     6601 gggcaagcca tagttcgccg tcaagctcgc gcagggtaaa ttcggatagt ccgtcttccg
     6661 tggtgtagat gatgatgtcg ttgttgtgca tagctttcct gtatctataa atggttaagg
     6721 ctacctgaaa atctacttct caaatccgcc cgctttcttt taaatattcc cgtaccaaca
     6781 ggcggttgat ggtctggatg tctgcgggtt gaacaggaaa ttgctgttcc gttctaaagc
     6841 gttttaatac gatgccctgc atttgttttt caaagtaatt ctggttgtcc aaaatctgct
     6901 ccatatccag cactctttgg tcggcgtcgg ttttgacgcc gtttagcgcg ttgaagactt
     6961 tgagtttgtc tccgtagagg gcggcgtttt ccatcaaccg cttgtggata cgtgcgtatt
     7021 tggcgtcgcc gccgtatttg tgccgcaaga ggtcgttttg gcggttcagc tctttgattt
     7081 tggcatagac ggtttcgagt gtggcgatgt ttgtctgcat ttcttcttgc ccgacttcgg
     7141 cgaggttctt tttcttgaag atgcgttcca gctcttcgcg caggctgatg tattcaaggt
     7201 cgtcttggtc gaagttgccc gccagccctt cgcgcacttt gcgcatgatg tcttttaaat
     7261 cgtcggcaag tttgagttcc gcctcgccga ttttgacgaa ttgaaagtaa acgtcttcca
     7321 gcgcttcatt gagcaaatgg gcggtgtcgc cttgctgtaa tttttcggca aggtttaagg
     7381 tatccaagcg ggcggcggtt tcgcggtaca aaagattgag cttgtcaaaa tccagatgcg
     7441 caagaaattc gtggctgcct tgcaggcgca ggatgttgta caactctttg gcggtttgca
     7501 gggctttttt taaggctaga agctgttttt tgtcttctat ttggctaatt tggctgcaaa
     7561 attcttcggc gttttcggta tcgaaatcaa acagggcgtt tttaatgtct gcaatttcct
     7621 gttcgatttc ttcggcggtt ttgaatagct ggctgtaact gccgatttcg tcgcccaatt
     7681 cgttggaaag ttcgtcccaa taggcgcggt tggttttgtc aaattcgcgt tcgatatcgg
     7741 caaaatcgac gacatagccg tagcggtaag atttgtaggt gcggttgacg cgggtcaggg
     7801 tttgtaaaag gttatgggct tttatcagtc tgcccaaata gagctttttc aggcgcggcg
     7861 cgtcaaagcc ggtcaaaagc atgttgtaca caaacaagat gtcggttttg cccgctttga
     7921 aatctttaac ccattggtcg cgttcttcct tagtgccgac gtcgtgcaat atcaacgcgg
     7981 cggtgaaatt gtggtctgag gccgtctgaa aatgctcaaa aagctggcgt gcctgttctg
     8041 cgctgtcgca aacgaccatg gcaccgaggc tctcgtcttg gttggttttt ctgaatttgg
     8101 cgaagtcgtc caagatgtag tcaagcatcg ggtggacgaa gtgcggatgg gcgtagattt
     8161 ctttgcggtc aaagctgcct ttttcgattt caagttgcgc cagtgcttct tgtaattgcg
     8221 ccttgtatcg gctgccgatt tcttcgcgta tcaggcgcag ggtgtagccg tcggcaatgg
     8281 aggcgttgta atagtatttg tggatgtagt cgccgaagag ttcgcgggtg ttgacgttgc
     8341 ctgcggttac gccgataagc ggcgtgccgg tcagcccgat tttgacggcg tttacgtcgg
     8401 actgattaag gttggcaaga aatgagcctt tggggttgta gctgcggtgt acttcgtcga
     8461 gaaaatagac gcgccggatg gcgaggtcgt agtcgttacg ggcgacaaca cgggggcgtc
     8521 tttgaatttt tggatgttga cgacggtgat ttccggtttg cctgcgtggt tgtgcagggt
     8581 ttgggcggat ttgatgtcgg caacgaaggc ttcgcggctg tcgatggtat ggacgattaa
     8641 gtcgcgggcg gtaaattccc gctgcgcctg tttcaataaa tcgagcctgt ccacgatgaa
     8701 atagaatttc ggcacgatac cctgtttggc gtaatagtgg gtcaggtaac gggtgttgta
     8761 ataggcaggc gcggttttcc ccgatccttg ggtatgccaa atcacgcctt ttttaccgcc
     8821 gttcgataaa tgtttttcaa tggcaagggt ggcaaacagt tgcggataac gcatgatgtg
     8881 tttttgcacc gaaccttgac ttgctttgac gtaagtcagc ccgtgttgca ggagaaaggc
     8941 aagacgttcg cggcaaagca gcgaagtcag gatgcggttg gtcggcgtat cgggcgattt
     9001 attgctgata aattcggggc tgtgtttgat aacgggcaga ttattgtctt gtagaacgtt
     9061 gttttcaagg tcgtctgaaa gcgtgtccaa taattcggta aggtttaatt tatgctcttc
     9121 gcggaagtag ttgaatacgg gcttgccgca agcagacgag gcgtaaaacg cgccttgtgc
     9181 cggctcggtt gcgccgtcgt cgtattccat gttgttggaa aaaatcatga attgggtgat
     9241 gttgataaaa cggcggaatt tgggattttt cgcacgtttg cccatgcgct cccgctcttc
     9301 gccgatgccg cctttattgt tgggcttttt gacttcgata aagaccaaag gcatgccgtt
     9361 taccagcagg gcaatgtcgg ggcggaatgc ttcgtcgccg ttgatacaag gcaactcagt
     9421 tacaacgtgg aaactgttgt tgtcaaaatt ttggaaatcg atcagcttct taccgccgga
     9481 ttgattggtg aggcgttcgt aaaatttttg ccccaaatct tcgttatcca gctctaagcg
     9541 tatgtcggca agcaggcggc gtgcatcatc cggcggcaaa tcgggattga tgcggcagag
     9601 gctgtctaca aagatttcgg gaaagatatt ggtttgtctg tcccaatttg cattttttag
     9661 cgaaagataa tcatagccca agcgcatcag gtgcaaaacg gcggggattt tgacgcggga
     9721 attttcgttg tgggtttggg acataaacgt aacgtaatgg gtcatcaaaa tttattgaaa
     9781 cggattataa cacctgaaaa aagccgatac gccttttcgg tgtaccggct ttgccatact
     9841 gttctgcttc agacgacatc gcttcatttt gcctttaatg cctcttcgtc cagcgatttc
     9901 aaccatgcca gcttttcgcc gattttgatt tccagcccgc gcgggacggg ttggtaaaag
     9961 tccggttcgt ccaagccgtc gggcatataa ctttcgccgg cggcgtaggc gttcggttcg
    10021 tcgtgggcgt agcggtattc gcgtccgtag cccaattcct tcatcagctt ggtcggggcg
    10081 ttgcgcaggt ggacgggcac ttcgtcgctg gcgttttctt tgacgaagcg gcgcatttgg
    10141 ttgtatgcct tgtagcccgc gttggatttg gcggcggcgg caaggtacag cacggcttgc
    10201 gccagcgcga gttcgccttc gggcgagcct aagcgttcaa aggtggcggc ggcatcgttg
    10261 gcgatttgga aggcgcgcgg gtcggcaagc ccgatgtctt cccaagcgat acgcacgatg
    10321 cggcgggcga gatagcgcgg gtcggtgccg ccgtcgagca tacggcaaaa ccaatacagc
    10381 gcggcgttcg gatgcgaacc gcgcacggat ttgtgcaggg cggagatttg gttgtagaaa
    10441 ctctcgccgc ctttgtcgaa acggcggatt tgcgcgccca agctgtcggc gagaaattcg
    10501 gtggtcagga ttttcagacg gcgcgtgtcg gcggcacgta aaagttgttc caacaaattc
    10561 aataatctgc gcgcatcacc gtcggcggta ttgacgagca gctcttgcac atcggcttca
    10621 atcgtgaaat cccggtattc gggcaaagcc aacactttgg caatcagttt cttgaggtcg
    10681 tctgaagaca aggattgcaa aacatatacc tgcgcgcggc tcaacagcgc gggattgact
    10741 tcaaacgacg gattttccgt cgttgcgccg ataaaggtca gcaaaccgct ttcgacatgc
    10801 ggcaaaaacg cgtcctgctg cgccttgttg aagcggtgga cttcatcgac aaacaaaatc
    10861 gtcgcgcgtc cctgctgcaa agcgatttca gccttatcga ttgccccgcg gatgtccttc
    10921 acgccggaaa acacggcgga aacaggcaaa aactgggcat tgaaactctg cgccaaaatc
    10981 cgcgccaacg ttgtcttgcc cacgcccggc ggcccccaca gcaacataga atgcggcttc
    11041 ccgccttcca ccgccacgcg cagcggcttg ccttcgccga ttaaatgctg ctgcccgatg
    11101 acatcatcaa gcgtatgcgg gcgcagacgt tcggcaagcg gtgcgtcggg ttctcggaca
    11161 aacaaatcgg tcataacggc tccgtcaaca ggttttcaga caatatgatt atacggcagg
    11221 gaaaggcggc gtgccgcata cggataccgc cccaccgttt gtactaagcc gatcttaggc
    11281 gtacaattgg gaaatttcag gaaatttata cgctatgcca tccatgcagc tttgcaaccg
    11341 gacccgcaaa gaccaggtcc cgttttaacc atgcggggaa gttaagatta cagtaaacaa
    11401 aactattttg ccttattgga agaaagggga acagttatga gaaattggaa cttattacca
    11461 ccggggaagg ggcagatgga gacttactgg tcaacggttc ttacggcatc cgttcttttg
    11521 cagacctaaa attggaatac aaacccgaat tatcggtgag aagcaatcat gacattattt
    11581 tcaacaatga ttttcaatct gttgacgtac aaccctattc cggaaatatc catttggatg
    11641 ccaaaggaaa aattcaattt aatttgaatg ttgccgataa ggtaacagat ccaaccacac
    11701 atcaagaaaa gttagtgaac gataacgctt tagtgcggta tgtcaacaat atcaccccct
    11761 ccgatgaaaa tgacaaaggc agcatatcct tccattcaaa aacaggcaat ttgttcaata
    11821 tgaacgttaa cgttcataaa ggcacacacc atttcggtat tcacaacctc cgcaatacca
    11881 atgttaatct gactgccgaa gacggcgata atgtggttgc gattaaaggg aagcttgccg
    11941 agcctatgcc ggcaggaaag ttgagagtac aagtttttta aggggtattg aaacggcttt
    12001 taaatccaat accgtattaa cggggaataa caataaaata tccatcacca ctgagggcga
    12061 cggcttggga ttgggcgtaa atggcctcca gtccaactcc ccctattccg gacacacgac
    12121 gggtactttc atcaataatc aaccaagcaa tactacccaa ccttcccttg acggcaccag
    12181 caccatcgaa ctcaaagccg tcaaggggaa taatgaagtt gatttaaatg taaaaaacca
    12241 cgcctccgta aaaggcatta tcacgtcgca ttctgcaaaa gcaacgcttg aagccgagga
    12301 ggataatatt gtccgggtta aaaatccgga caccaagacg gctttaatca atgcctggaa
    12361 agaaaaatac cccaataatt cgccagaacc atacagggtg gggggttcaa tccacgattg
    12421 acagcgatgt agttttgagt gcggcaaaca atatcgttga agtaggaggc agcccgatga
    12481 accaagaagg gattaccgct cacggaaatg ccacgattac cctcaaggcg aaagaaaaca
    12541 ataaaattac cgtggaaaac gccgcataca gcagcgacgg catttcgact ctgattaaca
    12601 gaacgggggc aagacccgga acaagggatg atggaaataa aatcatactg gaagccggcg
    12661 gcgataatat tgttaccatg aaatccggcg atgcggatgc ggattatgta aacaattcca
    12721 aagtattaac ggagacacca tattataaaa gcaaacgagg ttccaacggc atttttgcct
    12781 atggcgacaa atcgctggtc aaactgattg gcgagaataa tatcgttaag agtgaaatca
    12841 gtgaaaaatc taaggcatta aatgggggat ttcgccatat cggcatttat tcatggcaaa
    12901 acgcgaaagt cgaattgtct gcgaagagcg acaatatcgt acaaggcgga atttggggct
    12961 tatactccaa caactcctca atttccctca aggggaaaaa ataatgtgat ttcaaacccg
    13021 aaatataatg ttttcgccta caaaaaggca aaggtggatt tggtcaaatt accattgact
    13081 ccaacgttgc caatcttgca aggcaagatg gttcaattca tttgaattat aaagacgata
    13141 cccgtatcac aggggcaacc gtatctgata agggtttggt agccatcaaa cctttgaata
    13201 acacgaatat tgttgccgac actattcact ataaaggcga tgtcttggcg gtaaataagg
    13261 gtaaagtgga attagatttc acgccgaaca tccgtttagt gggacgtttg gataatttta
    13321 gcggcttaac cgattccaaa cataaaaatt tattcgaaaa ctatgttgca aatttagaca
    13381 gcaaaagtgc gggcgaaatt aactttaatt tagccaaaga cgcattatgg acgatgacag
    13441 gtcaaagctg gctggataaa ttggaaggac aaggcactat cgattttaat aatgatgcta
    13501 aaacaagtgg acgcgcctta catatcggtg aattggcggg tgccaataaa ttcttgatgc
    13561 atctgaataa agacggcatt cacagcgata tgctctatgt gaaaaaaggc acttcgacac
    13621 cgcaagaagt cgtcgtcaaa aatctgtccg aagtgctcga cagtatgaat tacggcgaac
    13681 gtttgcgttt cgctacagta acaaactcaa aaaatgaatt tgtgaacggt aaaaaatata
    13741 ttgacgatac gcaccttatg gaggatgccc tgactgtcga atactccgca cataacggcg
    13801 ataaaaacaa caaggatgac tataataaat cctttaacgg ctctgaaatg acggcggaaa
    13861 aagctggaga cgattatgtc aataaaacct ataccgacaa caggcaaaat gtctatttgg
    13921 tcaaacaggc taccggcaat ccgagccgaa atgtcaaaaa tatcaatgat atgttcgatt
    13981 caaccgcaca ttatgcgttc actttggata cttatgccaa acgcgaaggg gagcgggctt
    14041 tttcaacgtt ggataaaaaa gaaggcgatt ggataaggct gacgcatacc cgtgtgattc
    14101 aatccaatgc gtttaggttt cataacaacg attttgaaat cggatatgac cgattcagcc
    14161 tcaacgagca ggagaaaaaa cgcaaatggg gcataagtct cgactacggc cacggcagga
    14221 catcattatg gaatacgttt ggcaaggaca aaatcaggaa atatgaattg gctctgtaca
    14281 atactaccca atacatagat aaagaaggag acgaaacagg gtatatcgac aatgtattaa
    14341 aaataggaaa actgcgtaac cgtgtgattg cacgaaatca tatggggcaa ttatggggca
    14401 agggaaaata tagcaacacc ctattctcta tcagcaccga atacggccgc cgtaaatttt
    14461 tggatgacga taaattgtgg cggattacac cgcaagtaca gttgcaatat tcctatttga
    14521 gaggtaccgg ctatcggatc gataacggca taaacgtcaa tttaagccac gcaaacagcc
    14581 tgataggccg cttgggtttg gatgtcgtga gaaaatttga cggaggcaaa aaacttttct
    14641 atatcaaagg caatatcttt catgaatttt tgggcagtcg ttcctttaag gcatttgagg
    14701 gcaaaagtca ttatgctcaa aaatgaaata cgagggggac ttggtattcc gccggattgg
    14761 gctacaatgc gcggacaggc aaaaaaaacg aatgtctttg ccgatgcgga aaaaagagtt
    14821 ttccggcggg aaaaaaggat cgtacaacat ccgcttatcc gtttcccatc agtttgactg
    14881 aatgatatcg gggccgcgcc attattcggc aggccccatt ttattgccac acattgcctg
    14941 cccgattccc gtttcaacag caaacccttc caatattttg ttcaaacttg gatcgcggca
    15001 gatgggggcg tgcaagcccc gcaaggtttt tttggctttt acggaacagc acgcatttcg
    15061 ccaaacggca aaattgcatt aacggaattg aaaacttttg gaattgtgca aaacggcatt
    15121 tgcgtcagtt taacggcatt tccaaagggc attttaagcc gtattcgaga gaatgcggat
    15181 ggcgttttca gtgttttcag cgtgataaaa ttcaaatttc tattctaaaa caatcagcaa
    15241 agcaggattt atttcagttg tccgagacaa cgggcggctg caccgcctgc actccccata
    15301 ctttttcaac gcactcgagc aggagtgaga cgtggggatt atcctcgtat tcttccgcat
    15361 aaatgaaatt gagcggcata ctgtggcggc acgattcgat aatagccacg cccgcgcctt
    15421 cttttgccgc cgcttcggct ttgtttccct gcaccattgc cacacctata ccgcctgcaa
    15481 ccaaatcgat aagggtttgg gggtagtcgc acaatatctg tttttttggg tgagagccgg
    15541 ttgctgcgcc aaaactggtg caggtgcttc ctacttccgg acacgcccga catttctatc
    15601 catacgcatt cttgcaggct ctgcggaagg gagcgggtca gccgggaata ttggctttgc
    15661 gggcaaatca gcgcgtaggt caggttttgc aggaatacgc tgcggatgcc gccttgggcg
    15721 gcgttgccga gtataaagcc gccgtgcagg gttttgtgtt ggatgcgcga gaggatttcg
    15781 ccgctcattc cgtattggat gtgcaggcgc gttttggggg gctgtttgac cgatattgtc
    15841 cgtcagcgcg acgagttttg ccgaatcgat ggggtggata atgcccaaac tggtctcttc
    15901 cgaataatcg cctgccagcg ttttggcaaa atactccaac ttgtgtttgt attgcagcag
    15961 ggattccgct tcgggcaaca gtatttcgcc cgcccgcgtc aataccatgc ctttccccgt
    16021 gctcctgaac agcggcgtgc cgacatattc ttcaagggct ttaatttggg cggaaacggc
    16081 gggctgggaa aggaaaagtc gtttggcggt ttgggtaagg ttgccctcgt gcgcgacggc
    16141 gacaaatgat tttaattgta cggcatccat gtatccctcc ttgtgcggat gttttctata
    16201 tttgtgcaat cgaaatcttt taggcggatt gttgctgaaa attaactttt taatcaagtg
    16261 gtttgtaaat tgtatcagtt ttccggatga tgattattaa aaaaaaattg gttttattgc
    16321 ctgtttgggc tttaaatggg gttacggctt ccgaacgcag cccgtatcaa aaagaaaagt
    16381 catgcgcccc cttttacgag gcgcgatata taaggaggaa ggttatggaa aaacataatg
    16441 ggacttatcg ggatttacac cgtccggctt cggaatttgc gacgcgggac gaatatttgg
    16501 aacatgaatt gcagattatg caaccaaaac gctggcggcc caacctgccc tttcgcgatt
    16561 accgcttcga gtgggaggat ttgattcctg cgatggcggg aacgattgga aaagtggtga
    16621 tggtgggggg gcggcggcgt ttgccgcacc tttggggctg cctgacagct ttgtactgga
    16681 aaatgtgcgc tatgagcttt taatcgccgc cgcgtttatc ttattggtat cgggcttttt
    16741 tctgcccggc gccaacctgc ccggtacgca cgggccgctg attccgatga ttcccatcgt
    16801 tgtgtcggca ggcgggcatc ctttggcgtt cggcatttcg attgcggttt taggtctgct
    16861 gatggcttta tttcgcggcg gcagtattat ggcgaagctg acaagcaacg gcgtatgcgg
    16921 cggattatta ctctatttgg gctttatcgg cacgacgggg caggtaaaaa aattgttttc
    16981 gtgggcaggc ggttttaata tgccctacat cgcttttacc gtcattattg taacgattgt
    17041 gatgtacgct ttgttggagc attggaaaaa acgctggttg gccgtgcctt tgggatgctt
    17101 gattgccggt gtggcggcat ttgcattggg tgcgccgttt gagtttcaca ccgcccccgg
    17161 cctgcctcca atgagtcctg cttattggta gggtgaaaac agcggctggc atctggggtt
    17221 gccgacggca gaaagttttt tggttgtctt tccatttgcg gtattggctg ttgcaatgtg
    17281 gtcgcccgat tttttaggac atcaagtgtt ccaaaaaatc agctatccgg aaaaaaccga
    17341 taaggtattg atgaatatag acgacaccat gacaagttgt tctgtccgtc aagcagtggg
    17401 ttctatttta gggggtgcaa attttacctc ttcttgggga acttatatcg tgccggcatc
    17461 gattgccaaa cgccccattc cgggcggtgc ggttttaacg gcggttttat gtattatcgc
    17521 cgggttgtgg ggctatccga tggacttggc gatttggcag ccggtattga gcgtagcctt
    17581 gatcgtaggc gtatacttac cgcttttgga agcgggcatg gaaatgacgc gcaaaggcaa
    17641 aaccacccaa tccgccgcca tcgtggtgtt ctcttccgtc tggtcaatcc ggttttcggc
    17701 tgggcgttga cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga acgcagcgcg
    17761 caattaggtt ttgtcggacg agtattgata cccgcagtag gtttcttaat cttgtgtgtg
    17821 gcgatgggtg cggtcgggat gctgcccggt atccctccgt ttttggagca gttcaaatct
    17881 ttgggctagg ctgaaatcgc aaatgccgtc tgaaccggtt tcagacggca tttttgcaaa
    17941 caggcaaaat gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg
    18001 cttcaaccgg atattcaaac ggtttgctcc aacactcgga acggcgcata aaacgccgcc
    18061 cttcgcgtta tcccgaacgg ggcggctaat cagatcctat cgccataaaa ggcggggttt
    18121 caaccgaaaa ggaattgaga tgaataaaac cttgtctatt ttgccggcgg caatcttact
    18181 cggcgggtgc gccgccggcg gcaacacatt cggcagctta gacggcggca cgggtatggg
    18241 tggcagaatc gtcaaaatga cggtagaaag ccaatgccgt gcggaattgg acaggcgcag
    18301 cgaatggcgt ttgaccgcgc tggcgatgag tgccgaaaaa caggcggaat gggaaaacaa
    18361 gatttgcggc tgcgctaccg aagaagcacc taaccagctg accggcaacg atgtgatgca
    18421 gatgctgaac cagtccacgc gcaatcaggc acttgccgcc ctgaccgtca aaacggtttc
    18481 cgcctgcttc aaacgcctgt accgctaacc ttcttcagac ggcatgaggc agatgccgtc
    18541 tgaatccgtt ttcctgcaat ttcgacaaac gagaaaaatc atgagataca tcagcacgcg
    18601 cggcgaaacc gcacacaaac cgttcagcga agttttattg atggggcttg cacccgacgg
    18661 cggactgatg ctgccggaac attatccgca aatcgggcgc gaaaccttgg acaaatggcg
    18721 cggtttggct tatcccgaat tggcgtttga aatcatgtgc ctgttcgtta ccgatattcc
    18781 ggaggacgat ttgcgcgaca ttctgaaccg tacttacacg gaagcggcgt tcggtactaa
    18841 ggaaatcacc cccgtccgca cgctttcaga cggcatcaaa atccaagcct tgtccaacgg
    18901 cccgacgctg gcgttcaaag atatggcgat gcagtttttg ggcaatgcgt ttgaatatgt
    18961 tttaaacaaa gaaggcaaaa aactcaatat cttgggcgca accagcggcg atacgggttc
    19021 ggctgcggaa tatgccttgc gcggcaaaaa aggcgtgaac gtatttatgt tgtcgcccga
    19081 cggtaaaatg agcgcgttcc aacgcgcgca gatgtacagc ctgcaagacg ggaatatcca
    19141 caatatcgcc gtgaagggga tgtttgacga ctgccaggac attgtgaagg cagtgcagaa
    19201 cgatgccgcg ttcaaggaaa aataccatat cggtacggtc aattcgatca actgggggcg
    19261 catcgtcgcg caagtggttt attactttgc aggctatttc aatgccactt caagcaatga
    19321 cgaaaccgtc agcttttgcg taccgagcgg caacttcggc aacgtttgcg cgggacacat
    19381 cgccaaacaa atgggcctgc ctgtccgctg cctgattgtc gcgaccaatg aaaacgatgt
    19441 gctggacgag tttttcaaaa ccggcgcata ccgcccgcgc aacagcgcgc atacttatgt
    19501 aacctccagc ccgtctatgg acatttccaa agcgtccaac ttcgagcgtt tcgtgttcga
    19561 cctgatggat cgcgatcctc aggaaatcaa tacgctgtgg gcggaagtcg cggcaggcaa
    19621 aggctttgac ctgcggtttg ccttggacaa agtcggcggc aaatacggct ttacctccgg
    19681 caaatctacc cacgccgacc gcctcgccac catcagacag gtttacgagc aagacaaaga
    19741 actcatcgac ccgcatactg ccaacggcgt aaaagtcgcc cgcgaagtgc gcgaagcggg
    19801 ggaaacggtg gtttgtttgg aaaccgcgtt ggcggcgaaa ttcgatgcga ccatacgcga
    19861 agccgtcggc gatgtcgcca ttccgcgccc cgccgcgctg gaaggtttgg aaaacctgcc
    19921 gcagcgcgtg caaaccgtgc cgaacagtgc ggatgcggta aaaggcatca tcgaacaaac
    19981 ccttgcctga tgttccgtat gggcggcatt ccatccgaac ttaagattga aagaaaatga
    20041 ccttacctat gcaggaaacc cgtttttcta tactgctgga cgaattggcg gcaaaacagg
    20101 aggcaaccat cgccccctac ctgcttgccg acgggaccaa ggtatgggta cgcaaagcag
    20161 gcaggcacaa tgcgcgatgg cgttacgcgc tgctcggtat ggttgcccga tatttgaaac
    20221 tgggggtgtt gaagccggtt cccagcctcg gcggcgagcc tgccattgca accgaatcaa
    20281 aacgcctgta cgaattgcgt tcggcaggga tagccgttcc cgaattgctc gccgtccgga
    20341 aaaatgccct tatgttcggc aatttggaag gcatcccgct cgatacgcaa atccgccaag
    20401 aagccgaagc cggaaaggcg gacgcttggc ttgccggttt ggaagccatt gcacgcgtgc
    20461 ataaaaaacg gcagttcctc agtcaggcgt ttgcgcggaa tatgatgtgg gacggaaaaa
    20521 acatcagctt tttggatttt gaagacgacc cttccgaggt cctgaccata gcgcaatgcc
    20581 aagcccgcga ttggctctgc tacatccatt cgaccgcgct gatattgaaa aacggcggac
    20641 tgctggaagc ggctgcggaa aaatggggcg gcgtattgtc ggatcagcct gccgaaatac
    20701 aaaagctgat tgccggcacc gtcaaaccga tcctcccgat acgcaggctg gaacaccccc
    20761 gctggggacg ggacgcgctc aggctggcag cctcgatttc cttaatttcc ctcgccgata
    20821 tgccgccgta agccaccgcc cgccccacga tgccgtccga agcccttcag acggcatata
    20881 accaacgcgc atcaggcatt gtcgcaacca tccgtttcgt ttagaatttt gactttatcc
    20941 cgaatcgggc agacatttcc actctgccgc acgtcccgaa caagcactat ccatttcttt
    21001 tactgacagg aaacccaaat ggcagcattc aatacccaaa aagtattgtc cgtacaccac
    21061 tggacagacg catattttac ctttacctgc atccgcgacg aatcgttgcg ctttgaaaac
    21121 ggacagttcg tcatggtcgg gctgatggcg gacggcaagc cgctgatgcg cgcatacagc
    21181 gtcgcctccg ccaactggga agaacacctc gaatttttca gcattaaagt ccaagacggc
    21241 ccgctgacca gccgcctgca acacctcaaa gtcggcgacg aagtgttaat cagcaaaaaa
    21301 ccgaccggaa ctctagttgc ctgcgatctg aatccgggca aacaccttta cctgttgagt
    21361 accggtaccg gcatcgcccc tttcttgagc atcaccaaag accccgaaat ttacgaacag
    21421 tttgaaaaaa tcatccttgt acacggcgtg cgctacaaaa aggatttggc atactacgac
    21481 cgctttacca aagaattgcc cgaacacgaa tacctcggcg acttggttaa agaaaaactg
    21541 atttactacc cgattgtttc ccgcgaagaa ttcgaacacc gcggccgcct gaccgacctg
    21601 atggtaagcg gcaaactgtt tgaagacatc ggcctgccca aaatcaaccc ccaagacgac
    21661 cgtgccatgc tgtgcggcag ccctgcgatg ctgaaagaca cctgcaaagt tttggacgat
    21721 ttcggtctga cagtctcccc gaaaaccggc gtgcgcggcg actacctgat tgagcgcgcg
    21781 tttgtggatc aataagccgc ttgaaaatgc cgtctgaacg gcatcagcga taaaaaaacc
    21841 cgaatcctgc caatcaggat tcggggtttt tatagtgggt aaaaattaga aatgcacatt
    21901 tccgtcattc caacacaggt gggaatccgg acttacctgt ttcattatcg tttgaaaata
    21961 aaagagaaac caaacatata gattcccgct tgcgtgggaa tgacgacggt gttttgccgt
    22021 agccaaagca ggaaaacatc cttattgtca tttcatcatg ataggacagt aaggatgtta
    22081 aaaattaatt atcattatat gattgatata taataaaaat tatcccaata agaaatgtta
    22141 tgcagaaaat aaaatgaaaa ggatggtttt cattgaaaat ggttgttctg ccaccgtcat
    22201 acacagaacc ggtatttgga aaaaaaaata atccatattg acatcattat tccatttatc
    22261 gcataaatcc actttttaat ttttttcctc attaatataa tctcttattt aataaaatca
    22321 aatacctagc aattcgatct caaattttaa acgccaacgt gtttgagcgg taacaatgca
    22381 tactttaagt ttgtaatcct tcatcgtctc atttttgttt taatcgacta tacgtctaaa
    22441 gcaacgccgc ccgtcattgt tcggacagta ttttttcaga cggcattttc ccccgtttgt
    22501 gaaggcgcaa ggcattgccc aaaaccgcca gcgaactgaa actcataccc agtgcggcta
    22561 tccacggttg gacatagccc aaaacggcaa gcggtacggc gatgatgttg tacgcgcccg
    22621 cccatatcag gt
//
LOCUS       DAXDZG010000032        22086 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXDZG010000032 DAXDZG010000000
VERSION     DAXDZG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22086
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.032"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            96..917
                     /locus_tag="ACFB78_001048"
     CDS             96..917
                     /locus_tag="ACFB78_001048"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC1269051.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1003..1665)
                     /locus_tag="ACFB78_001049"
     CDS             complement(1003..1665)
                     /locus_tag="ACFB78_001049"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC1269052.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1703..2530)
                     /locus_tag="ACFB78_001050"
     CDS             complement(1703..2530)
                     /locus_tag="ACFB78_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC1269053.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB78_001051"
     CDS             2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB78_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC1269054.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3809..4945
                     /locus_tag="ACFB78_001052"
     CDS             3809..4945
                     /locus_tag="ACFB78_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1269055.1"
                     /translation="MKKTLVAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIYN
                     WSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIK
                     AGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALG
                     TDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAA
                     VDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLT
                     PKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMD
                     EKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5040..5306
                     /locus_tag="ACFB78_001053"
     CDS             5040..5306
                     /locus_tag="ACFB78_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1269056.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKISNTKHQTPNTKHQTPNIYIRITKDCRRREIPE"
     gene            5279..6037
                     /locus_tag="ACFB78_001054"
     CDS             5279..6037
                     /locus_tag="ACFB78_001054"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC1269057.1"
                     /translation="MSEARNTGIKNSNGKYIAFIDSDDFINCQVLLDFLGKDDSDMPD
                     VVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSAWNKIIKRELI
                     IREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSITGTVSEKGIKS
                     LLYILEKNAKMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACDSDIKRRAYHLRFML
                     LKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            6078..6608
                     /locus_tag="ACFB78_001055"
     CDS             6078..6608
                     /locus_tag="ACFB78_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC1269058.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(6776..8371)
                     /locus_tag="ACFB78_001056"
     CDS             complement(6776..8371)
                     /locus_tag="ACFB78_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC1269059.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(8478..8873)
                     /gene="hisI"
                     /locus_tag="ACFB78_001057"
     CDS             complement(8478..8873)
                     /gene="hisI"
                     /locus_tag="ACFB78_001057"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC1269060.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(8904..9671)
                     /gene="hisF"
                     /locus_tag="ACFB78_001058"
     CDS             complement(8904..9671)
                     /gene="hisF"
                     /locus_tag="ACFB78_001058"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC1269061.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(9684..10421)
                     /gene="hisA"
                     /locus_tag="ACFB78_001059"
     CDS             complement(9684..10421)
                     /gene="hisA"
                     /locus_tag="ACFB78_001059"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC1269062.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(10454..11092)
                     /gene="hisH"
                     /locus_tag="ACFB78_001060"
     CDS             complement(10454..11092)
                     /gene="hisH"
                     /locus_tag="ACFB78_001060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC1269063.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(11222..12724)
                     /gene="pta"
                     /locus_tag="ACFB78_001061"
     CDS             complement(11222..12724)
                     /gene="pta"
                     /locus_tag="ACFB78_001061"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC1269064.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(12909..13967)
                     /gene="fbpC"
                     /locus_tag="ACFB78_001062"
     CDS             complement(12909..13967)
                     /gene="fbpC"
                     /locus_tag="ACFB78_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC1269065.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(13988..15505)
                     /gene="fbpB"
                     /locus_tag="ACFB78_001063"
     CDS             complement(13988..15505)
                     /gene="fbpB"
                     /locus_tag="ACFB78_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC1269066.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(15573..16568)
                     /gene="fbpA"
                     /locus_tag="ACFB78_001064"
     CDS             complement(15573..16568)
                     /gene="fbpA"
                     /locus_tag="ACFB78_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC1269067.1"
                     /translation="MKTSIRYALLAAALTATTPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(16820..17300)
                     /locus_tag="ACFB78_001065"
                     /pseudo
     CDS             complement(16820..17300)
                     /locus_tag="ACFB78_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(17358..18734)
                     /gene="argH"
                     /locus_tag="ACFB78_001066"
     CDS             complement(17358..18734)
                     /gene="argH"
                     /locus_tag="ACFB78_001066"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC1269068.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(18753..19622)
                     /gene="galU"
                     /locus_tag="ACFB78_001067"
     CDS             complement(18753..19622)
                     /gene="galU"
                     /locus_tag="ACFB78_001067"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC1269069.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(19650..20249)
                     /gene="rdgB"
                     /locus_tag="ACFB78_001068"
     CDS             complement(19650..20249)
                     /gene="rdgB"
                     /locus_tag="ACFB78_001068"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC1269070.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(20242..20475)
                     /locus_tag="ACFB78_001069"
     CDS             complement(20242..20475)
                     /locus_tag="ACFB78_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC1269071.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(20583..21116)
                     /locus_tag="ACFB78_001070"
     CDS             complement(20583..21116)
                     /locus_tag="ACFB78_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC1269072.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(21218..21676)
                     /gene="nudB"
                     /locus_tag="ACFB78_001071"
     CDS             complement(21218..21676)
                     /gene="nudB"
                     /locus_tag="ACFB78_001071"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC1269073.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            21887..21963
                     /locus_tag="ACFB78_001072"
     tRNA            21887..21963
                     /locus_tag="ACFB78_001072"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21921..21923,aa:Pro,seq:ggg)
BASE COUNT         4676 a         5460 c         6622 g         5328 t
ORIGIN      
        1 gggtgtaatc cgaggtaggg gcggctgggg tgcttctccc ttgtctgccg ctgctgttat
       61 gatgatattt ttattctgta tttaaggagg gggtgatgag cagtccgcgc caagtgtttt
      121 atatttccga ccgcaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
      181 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
      241 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
      301 ttgtcagtgt ggttgatgac gaaatccgcc ggattattaa aggggcggat gcttttcaga
      361 ttaatttctt tgagactttt ttgggactgt tggagaagga actcaatacc gaagcaacgg
      421 tatccgggca ggggcatcac agtatcggca atacgaagcg ttacgatgcg cgtatggagg
      481 cggtcaattt ttctttgaac catgatgacg gggtcagcga taagaacctt caggaggcgg
      541 atgtgattct gatgggcgtg tcccgttcgg gcaaaacgcc gacctgcctg tatctggcgt
      601 tgcaatacgg tatccgtgcg gcaaactatc cgctgattcc cgacgatttg gaatcggccg
      661 atctgccgcg tatggtcaag ccgtataaag acaaactgtt cgggttgact atccagccgg
      721 aacgtttgca ggccatccgt caggaacgcc gcccgaattc ggcttatgcg cgcatcgaca
      781 cctgccgcag cgaagtggcg gacgcgcaga gtatgttcag acggcatggc attccgtttg
      841 cgaatacgac ggacaagtcg gttgaggaac tggcggtaca catccttcag gcgtgcaagc
      901 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg tggaaatgcc gtctgaaatc
      961 aggtttcaga cggcagtttt atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     1021 cagacattcg agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     1081 atcgtcttca tcggaaatat agccccattc ggcgaggacg gttttcatac cggcgttgcg
     1141 tccggcttgg atgtcgcgtt ccgcgtcgcc gacgtagagg gtgtgttgcg ggtcggcgtg
     1201 gatttttccg caggcgtgca gcatgggttt gatgctgggt ttgggttcgc cgcaggtgtc
     1261 gccgctgacg acggtggcgg gcggaacggc gaatccgagt ttgggaacga gtttgtcggt
     1321 gaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgt cgagttcggc
     1381 gatgagttcg ttcacgccgt cgaagagagt ggtgtcttgg gcgtagcggc tgtcgtattc
     1441 ctccagatat tcggtgcgcc atgcggtata gtcggggtgt tcgggcgtga tgttcgcgcc
     1501 gagtttgagc aatcctgccg cgccgtggct ggcttgggtg cggatttcgt ccatgctttt
     1561 ttcgggcagt ccgtggcggg cgagttgggt gttgagtgcg ccgccgaggt ctagggcggt
     1621 gtcggcgagc gtgccgtcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttttt
     1681 ataaagtgcg ggacgaaagg tttcagacgg catgtttatt ttgtttcaaa ccctgctcga
     1741 aatcttccaa catatccaat tcgaagcggc tgaagcccgc tttttcccgc gcttcgatgt
     1801 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggcga
     1861 caggttccaa accgcgttcg cggcagaggt gttgatacca ccggttgccg atggcgacgt
     1921 gtcccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa tcgccgcgct
     1981 gctccacctt cgcgcgtatc cccggcgtaa cgtccagtcc gcgcgcctcc aacacgcgcg
     2041 gtaccaacgc catacgcaac agcgggtcgt aggcggtttt gtacgccata tcccacaaat
     2101 ggttgtgcgc ctcgaaactg ccgtaatcga agccgaaagc gcgcaatctg tcgcgcacca
     2161 ggcggaagtg gtacacctcc tccttcgcca ctttcaccca gtcgcggaca aactgaaacg
     2221 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     2281 gcgtaatcgc gtgcagcatc gccgcatagc cttctgccgt gttcattttg cgcggggtca
     2341 gttgcgaggg cgcgaccaaa acaggcgtgt ccggccgtcc cgcgcggggg aagtccgccg
     2401 gcggcgcgtt tgcctccgcc ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca
     2461 gccgtccttt ttcatcgggg tcgcccgaaa gcagggcgcg ttccggcaaa gcataaatat
     2521 cgggtttcat ctcaagtccg ccgtgttcgg aaaacgaata ttatagcgtt taaaaaaaac
     2581 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     2641 gattaacaaa aaccagtacg gcgttgcccc gccttgccgt actatgttgt actgtctgcg
     2701 gcttcgtcgc cttgtcctga tttttgttaa tccactataa cgcggcacac aattgaaagg
     2761 gcagcgtggc gcgcctgctt tttccgagcg gtcaaaaaaa tcagccctcg gaaaacgcgg
     2821 tttgcaaaat gcaaaccgcc cgtaacgccg cccgtatgat tgttttgccg cgccgatact
     2881 ttgcgccaca cccatcccga caaggaaaaa taatgatgaa accgcacaac ctgttccaat
     2941 tcctcgccgt ttgctccctg accgtcgccg tcgcttccgc acaggcgggc gcggtggacg
     3001 cgctcaagca attcaacaac gatgccgacg gtatcagcgg cagctttacc caaaccgtcc
     3061 aaagcaaaaa gaaaacccaa accgcgcacg gcacgttcaa aatcctgcgc ccgggcctct
     3121 tcaaatggga atacactttg ccctacaaac agactattgt cggcgacggt caaaccgttt
     3181 ggctctacga tgttgatttg gcacaagtga ccaagtcgtc ccaagaccag gccatcggcg
     3241 gcagccccgc cgccatcctg tcgaacaaaa ccgccctcga aagcagttac acgctgaaag
     3301 aggacggttc gtccaacggc atcgattatg tgcgggcaac gcccaaacgc aacaacgccg
     3361 gctaccaata catccgcatc ggcttcaaag gcggcaacct cgccgccatg cagcttaaag
     3421 acagcttcgg caaccaaacc tccatcagtt tcggcggttt gaataccaat ccccaactct
     3481 cgcgcggcgc gttcaagttt accccgccca aaggcgtgga cgtgttgagc aactgatgcc
     3541 ggccgccccg atgccgtctg aacgccgccg aggcttcaga cggcattttc acgcaggcgg
     3601 aacaatgtcc cgcattaccg cccgatcggg caacggaacg gcaaatccgt gaaaattaac
     3661 ggttgcgccc cggctgtttt tgccgtttaa tgcaaacctt gctgcaccaa gggccaagaa
     3721 agccgaccgg ccgcccccac agcttccgat gcaggcggcc cgtccgtccc tgcaatgttt
     3781 tttatttttg aacgaaaggt cgaaaaccat gaaaaaaaca ctggtggcgg caatcctgag
     3841 ccttgccttg actgcgtgcg gcggcggaag cgataccgcc gcccaaaccc cctccgccaa
     3901 gcccgaagcc gaacaatcgg gcaaactcaa catctacaac tggtcggatt atgtcgatcc
     3961 cgaaaccgtc gccgcctttg aaaaagaaac cggcattaag atgcgttccg actattacga
     4021 cagcaacgaa acactggagg caaaagtcct gaccggcaaa tccggctacg acctgaccgc
     4081 gccgtccatc gccaacgtcg gccggcaaat caaagcgggc gcgtatcaga aaatcgacaa
     4141 ggcgcaaatc ccccattacg gcaacatcga taaagatttg ctgaaaatga tggaagccgt
     4201 cgatccgggc aacgaatacg ccgtccccta tttctggggc atcaacacct tggcaatcaa
     4261 cacgcggcag gtgcaaaagg cattgggtac ggacaagctg cccgaaaacg agtgggattt
     4321 ggtgttcaaa cccgaataca ccgccaaact caaatcctgc ggcatcagct atttcgacag
     4381 cgcaatcgaa cagattccct tggcgttgca ctatttgggc aaagacccca acagtgaaaa
     4441 tcccgaagac atcaaagccg ccgtcgatat gatgaaagcc gtccgaggcg acgtgaaacg
     4501 cttcagctct tccggctata tcgacgatat ggcggcgggc aacctgtgtg ccgccatcgg
     4561 ttacggcggc gatttgaaca ttgccaaaac ccgtgccgaa gaagccgcaa acggcgtgga
     4621 aatcaaagta ttgaccccga aaaccggcgt aggcgtgtgg gtggattcct ttatgattcc
     4681 gcgcgacgcg caaaacgttg ccaacgccca ccgctatatc gactacacgc tccggcccga
     4741 ggtggctgcg aaaaacggca gcttcgttac ctacgcgccc gccagccgtc ccgcgcgcga
     4801 gctgatggat gaaaaataca cctccgacgc atcgattttc ccgaccaaag aactgatgga
     4861 aaaaagtttc atcgtatcac ccaaatccgc agaatccgtc aaactgggcg tgaagctgtg
     4921 gcaagggctc aaagcgggca aataaccgga atccctgccg tctgaaacct ttcgggcggc
     4981 aggaaacggc gtgtccgcat taagtcagga ttaggattat ttagaaagat gagatggata
     5041 tgaatttaag tattgtcgtt cctatttata atgtcgaaag ttatttggaa gcgtgtttaa
     5101 attctataga acctatatta agtaatgaaa atgtcgaact tattcttgtg aatgacgggt
     5161 caaaagacgg aagtgaagat atatgttata aatatataga taaaatatca aacaccaaac
     5221 accaaacacc aaacaccaaa caccaaacac caaatatata tatcaggata accaaggatt
     5281 gtcggaggcg agaaataccg gaataaaaaa ttcaaatgga aaatatatag catttattga
     5341 ttcggatgat tttattaatt gtcaggtttt gctggatttt cttggtaaag atgatagtga
     5401 tatgccggat gtggtttttt taaatgcagt taaatatgat aagggaagag tttcatattt
     5461 tggcgaagat tatcagcctg aaaaaatatt aaatcaatcc aaagtcgaag ttttgaaagg
     5521 attgtgccga tttagaaaat ttccgggttc ggcgtggaat aagattataa aaagagaatt
     5581 gattattaga gaaaaactgt tttttgaaaa gggaatttat tctgaagata ttgaatggtc
     5641 aatgaggttg tttaatgcgg caacaacttt ttcttatttg gacggttgtt attactatta
     5701 tcggcaggga agaaaagatt ctattacggg aactgtttcg gaaaaaggta taaagtcatt
     5761 attatatatt ttggaaaaaa atgcgaaaat ggaatttaat agggatatat cgagttatct
     5821 ttattctttt ctttcctacg aatatctcgt tttgcttttt ataatgacga gtaaaaatat
     5881 agcgtgtgat tctgatataa aaagaagggc atatcattta aggtttatgc tgttaaagtc
     5941 caataagctg atatataagc tgatattccc gataatcaca ttattcgggg tcgatattac
     6001 aggcaggatt ttaaaagcaa tcagggggaa tatttaataa atcctttaac aatatatacc
     6061 ttaccgaagg aggaaaaatg aacgcaatca gaactttcca aaaccgcatg cccgaaatcc
     6121 acgaaacctg catgatagac gaggcgtgcg tcgtcatcgg cgaagtctcg cttgccgaag
     6181 atgtttccgt gtggccgtgc gccgtgttgc gcggcgatgt gaacagcatc accgtcggcg
     6241 cgcgcagcaa tatacaggac ggcagcgtcc tgcacgtttc ccacaaaacc gccgccaaac
     6301 ccgaagggtc gccgctggtt atcggcgaag acgttaccgt ggggcacaaa gtgatgctgc
     6361 acggctgccg aatcggtaac cgcgtcttgg tcggtatggg cagcaccgtt ttggacgatg
     6421 ccgttattga agatgacgtg atgatcggcg cgggcagcct cgttccgccg cgcaaacgtt
     6481 tggagggcgg ctatctttat atcggttcgc ctgtgaggca ggttcgtctg ctgaccgatg
     6541 aggaaaaagc ctttttgaaa tattccgccg cacattatgt gaagctgtcg aaacagtacg
     6601 ggatgtgaaa tcacatcggc gttcttacgt cagtcccaaa ttcacgcggt tgggatgccc
     6661 gataacggta tccgatgcgc cttgattttg accggctgcg tttgagttgc aggcaaaaat
     6721 gccgtctgaa agcctttttt tcggcttcgg acggcatttt attgccgatt tctttttaga
     6781 gtttgaccga atgttcgcgc gtttcgtgga acacgatgtc cggccaacgt tcttgcgtca
     6841 accccaaatt cacgcggttg ggggcgaggt aggcgaggtt gccgcctgcg tcgatggcga
     6901 ggttgcctgc gttggctttt tcaaattccg ccagtttttt cttgtcgtcg cacgataccc
     6961 agcgcgccga ccagatggat gcgctgtcga acacggcttc cacaccgtat tcgttggcga
     7021 ggcgtgaggt tacgacttca aactgcaaca cgccgaccgc acccaaaatc aaatccgcgc
     7081 cgctcatcgg tttgaatact tgaaccgcac cttcttcgcc gagttgttgc aaaccttttt
     7141 gcagttgttt gattttcagc gggtttttga tgcggacgct gcggaacagt tcgggtgcga
     7201 agaatgggat gccggtaaac gccagttgtt cgccttcgga gaagctgtcg ccgatttgga
     7261 tgttgccgtg gttcgggatg ccgatgatgt cgccggcgta ggcttcttcc gccagttcgc
     7321 ggttgtgcga catgaaggtt actacgctgg aggcggcgat ttcgcggttg atacgcaggt
     7381 gtttcatctt catgccgcgc tcgaatttac cggagcagac gcgcaagaag gcgatacggt
     7441 cgcggtgttt cgggtccata ttggcttgga ttttaaagat aaatccggaa aatttcggct
     7501 cgtccggccc gaccatgcgc atggtcgcgt cgcgcggttt cggtgcgggt gcccagtcaa
     7561 tcaatgaatt gaggatttcc tgaatgccga agttgttaat cgcagagccg aagaacactg
     7621 gcgtgagttc gccggcgaga aattcgtcga gattaaattc gttggaagcc gcctgcacca
     7681 attcgatttc gtcgcgcaac tgctggattt ccaacggaaa gcgttgttcc aattcgggat
     7741 tgtttatgcc tttgatgatg tcgaactcgt gcggcaggcg ttcgccgccc gcttcaaaga
     7801 gatagatttc gtcgttcagg atgtggtaca cgcccttgaa gtttttgccc ataccgatcg
     7861 gccaggtaac gggcgcgcag cggatttgca ggatgtcttc cacttcgtcc aagagttcca
     7921 aagaatcgcg cacttcgcgg tcgtatttgt tcatgaaggt aacaatcggc gtatcgcgca
     7981 ggcggcagac gttcaagagt ttgatggttt gcgcttccac gccttttgcc gcgtcgatga
     8041 ccatcaaggc gctgtccact gcggttaaaa cgcggtaggt gtcttcggag aagtcttggt
     8101 gtcccggcgt gtccaagagg ttgacggtgt ggtctttgta gtcgaactgc atcacgcttg
     8161 atgccacgga aatgccgcgc tgcttctcga tgtccatcca gtcggaggtg gcgaatttgc
     8221 cggttttctt acctttcacc gtgcctgcgc tttgaatcgc gcccgaaaac agcagcagtt
     8281 tttcggtcag cgtggtttta cccgcatcgg ggtgggagat gatggcaaac gtgcggcggc
     8341 ggcgcacttg gtcgaggatt tcttgggaca tggttttctt tgcaaaaagg ttcaggccgc
     8401 ttttcagacg gcccggacag tgtttgagac ggcgaaattg tacaaaaaaa tgcctgataa
     8461 ttcaatgttg gaggcggtca gtgcgtgctg ccgtaaatct ctttttcgtc tttcaggacg
     8521 gcatcggcgg tttcccacgc accgccgttc cattttttgt aaaagcagct ttcgcgcccg
     8581 gtgtggcagg cgatgccgcc gttttgggcg atgagcatca caatggtatc gccgtcgcag
     8641 tcgaggcgca gttcgcggac tttttgcgtg tgtcccgact cttcgccctt catccattgt
     8701 ttttggcgcg aacggctgta atagtgggca aagccggttt cgacggtttt ttgcagggct
     8761 tcggcgttca tccacgccac cattaaaaca cgtttggttt cggcatcttg ggcgatggcg
     8821 caaaccaaac ctttttcgtc aaatttgacg gcttcaagca ggtttttatc catatttcct
     8881 ttcagacggc atagtcgggg cggtcagagg cgcacttcga tgccggcttc gcgcatagtg
     8941 cgtttggctt cgcggatggc gatttccccg aaatggaaaa tgccggcggc aagtacggca
     9001 tcggctttgc cttcggttat gccttcaatc aggtgccgga cattgccgac cccgccggag
     9061 gcgatgacgg ggatgtcgac ggcttcggca acggcgcggg tcagcggcag gttgaaaccc
     9121 tgtttcgtcc cgtccctgtc cataccggtg agcaggattt cgcccgcgcc gcgtttttgc
     9181 atttcgaccg cccattccac cgcatccaaa ccggtcggat tgcgcccgcc gtgggtaaag
     9241 atttcccagc gtgtgttttc ggggttgacg gctttggcat cgacggcggc gacgatggct
     9301 tgcgaaccga aaaatccggc ggcttcgtta attaaatcgg gacgggtaac ggcggcggtg
     9361 ttgatgctga ctttgtccgc gccggcattg agcaggcggc ggatgtcggc aacggtgcgt
     9421 acgccgccgc cgacggtcag cgggatgaag acttgtccgg caacctcttc gatgatgtgc
     9481 aggatggtgt cgcggttgtc ggatgaggcg gtgatgtcga ggaaggtcaa ttcgtccgcg
     9541 ccttcgccgt tgtagcgttt ggcggcttcg acggggtcgc ccgcgtcgcg caaaccgatg
     9601 aagttcacgc ctttgacgac gcgcccgtct tttacgtcga gacaggggat gatgcgtttt
     9661 gccagtgcca taatcggatg cctttagtcg agggaatctg ccagttgctg cgcttgggca
     9721 aaatcgatgc tgccctcgta aatcgcgcgg ccggtaatcg cgcctgctac gccatgtttt
     9781 tcggcggcac acagggcggg gatgtcgtcc aagccggtca gtccgccgga ggcgatgacg
     9841 ggaatgcgga cggattgggc gagtttgacc gtcgcgtcga tgttcacgcc gctcatcata
     9901 ccgtcgcgcc cgatgtcggt gtagatgatg ctgttgacac cgtcgtcttc aaagcgtttt
     9961 gccaaatcaa ttacatgatg cccggttacg gttgcccagc cgtcgatggc ggccataccg
    10021 tctttggcat ccagtccgac gataatcctg ccggggaagg ctttgcacgc ttcgcgcacc
    10081 aagtcggggt ttttgactgc cgccgtgccg ataatcacgt cgttcagtcc caaatccaaa
    10141 tattgtccga tggttttcaa atcgcgcatg ccgccgccga gctgcacggg gatgtctttg
    10201 gcgacggcgg cgaggatgtc tttgatggcg ggcaggtttt gcggaacgcc ggcaaatgcg
    10261 ccgttcaaat ctaccaggtg caggcggcgc gcgccttgtt cgaaccagtg cagcgcggtg
    10321 tcggcgggcg aatcggaaaa gacggtcgcc tcttccatca gcccttgttt caagcggacg
    10381 caacgtcctt ctttcaaatc gatggcgggt atcagcagca tagtttttct ccttgtgcgg
    10441 ggccgtgtcg ggcttaccag tttaaaaagt ttttcaacat cgtcagcccg gcatcgtggc
    10501 ttttttcggt gtgaaattgc gtggcgaata cgttgtcttt gccgacgatg caggcaaacg
    10561 gggacgggta gtcgctttcg cccaatatgg tttcgggatt ttcgggggcg aaatagtagc
    10621 tgtggacgaa ataaaaacgc gtgttttggg ggatgccttg aaaaagcggg tggttttggg
    10681 tttggcgcac ggtgttccag cccatatgcg ggactttcag acggcatccc tgcgggtcgc
    10741 ggaggtcgcg ggcaaagcgt ctgactttgc cgccgaacca gcccaagccg tcggtgtttc
    10801 cttcttcgct gtggtcgaat aaaagttgcg caccgacgca gattccgaaa aacggtttgt
    10861 tttttaaggc atctttgact gcctcgtcca agccgccgcg cgtcagtgcc gccatacagt
    10921 cgggcatcgc gccctgcccg ggaaaaatga ctttgtcggc gcgggacacg cggtcggggt
    10981 cgccgcttaa aaagattttg gtattttttc cggcaagctg ccccgccgtc cggacggatt
    11041 tcaatacgga atgcaggttg cccataccgt aatcgataat ggcggtttgc atggcttcct
    11101 cctctttttt gcaatatggc tgcgatttta acaaacaaat gtgccgggct gataaaaatg
    11161 ccgtctgaaa acgggaatct gtcttcagac ggcatagggt tcaaacccgg aaagccgttt
    11221 gtcagccttc catttgtttt gcctgaacgg cagtcagggc gatggtgaac acgatgtctt
    11281 ccaccagcgc gccgcgagac aggtcgttga ccggtttgcg caggccttgc agcagcgggc
    11341 cgacgctcaa gacgttggca ctgcgttgga cggctttata ggtgcagttg ccggtgttca
    11401 ggtcggggaa gaccaaaacg gttgcctgtc ctgccaccgg gctgcccggg gctttggatt
    11461 tgcctacgcc cggcacggtt gccgcgtcat attgcagcgg gccgtcgatg gcgaggtcgg
    11521 ggcgtttttc ccgggcaagt ttggtggctt cgatgacggt atcgacatcg gggccgctgc
    11581 cggagttgac ggtggagtag gaaatcatcg ccactttcgg gtcgatgccg aaggcttttg
    11641 cggaatcggc agactggatg gcgatgtcgg caagctgttg cgcggtcggg ttcggattaa
    11701 ccgcgcagtc gccgaacacc agaacctgat ttggtagcag cataaagaat acgctggata
    11761 cgaggcttgc gcccggtgcg gttttaatca gttgcaaagc ggggcggatg gtgttggcgg
    11821 tggtgtgaac cgcaccggat accaaaccgt ccacatcatt ttgcgccatc atcatcgtac
    11881 cgagtaccac ggtgtcttgc agttgcttgc gcgcgtcttc gggtgtcagg cctttggatt
    11941 tgcgcagttc gcacatcggc tcgacgtatt gttcgaccaa tgaggcggga tcgatgattt
    12001 ccaaagagtc gggcaggctg atgccgcgtt ctttggcaac ggcttcgact tcttcgcgtt
    12061 tggcaagcag gacgcagcgg gcaatgcctt tttcgtggca gatggcggcg gcttggacgg
    12121 tgcggggttc tgcgccttca ggcaggacga tgcgtttgcc ggcttggcgg gcgaagtcga
    12181 tcaggttgta gcggaattgc gccggcgaca ggcgttttgc ttcgcggcct gccaatacgg
    12241 atacgtcttt cagcgcgtcg ctcgaaccga agaaggtcag gccggttttc tcggctgccg
    12301 cttcggcaac ggaggctgcc gcaccgtcca cgacaaaacc ttccaatacg cccggcgcgg
    12361 cggcgaagaa ctgtttggca aggttcaatt ggtgtgccag cgcgtcggca tcggtattgt
    12421 cggaacggac ggcgaagacg gctgccgcgt caagggacaa tgccagttcg acgtttttgc
    12481 ctgcgagata gattttgtcg gcatcgggcg cgatgccttc gatgacgagg ttggcggcat
    12541 cgagtgcggc aactttgccg accagtgcgt cgaaccagtc gtcgcttttg ccttgcgcga
    12601 gcagggtttc ggcggttgcg tcaacggctt ggaaaacttg tgcgtccagt gcttttgcaa
    12661 aggcttgtgc ggcggcggag gtgttcagtc cggcagatac gggtacgacg agtacttttg
    12721 ccataatata tcctttcgta tgctgcggtg tgcggcatat gtggttggaa ggggcggcat
    12781 atgggcagaa acggctgcct gcgtgccgtg cgtgccgtgt ttggcttggg gcgcgcaggt
    12841 tgaatatagc aaacaaattc tgtttccaac aagataaata tccgcaggct tgtggatgct
    12901 gccgcctttc agagggtatt tccggggaag aacagggcgg gaccgtccaa atggaggacg
    12961 gcggaaatgc cgtctgacag ggtgggggcg gaagggaggt tgagcgtgag gacggtttgt
    13021 ccggccctga ggctgatttc ggtatgccgc gctttgggcg tggttttgag aaccacggcg
    13081 tgaatggagg cggcgggtgc ggaatggggg tgaaggctga actgttccgg acggatgagc
    13141 agtgtaccgc gcgtgcctgc gggtgcgccg ctttggacgg gcaggcggcc caatctgcaa
    13201 tcggcggtgc cgtcggcgtt gagcgcggcg gggaacacga tgccttcgcc gataaacagg
    13261 gcggcatcaa ggtcggcagg ttgtcggtac aattcgtgag ggcttgcggt ttggaggatg
    13321 cgcccctgtt tcatcacggc aatccggtcg gcgtattgca gggcttcttc gcggtcgtgg
    13381 ctgacaaaaa cggcggattt tccgttggcg cgcagggcgg caatcatgtc ttcgcgaatc
    13441 tggcggcgca actgttcgtc cagcgcgctg aagggttcgt ccaacaaaat cagttcgggg
    13501 tcgggggcga gggcgcgggc gagggcgacg cgctgttgtt gtccgcccga aagttcgtgc
    13561 ggatagcgtc cggcaagttc ggaaatgccg gtcaattcca acatggcttc gatgcgctgt
    13621 cgctcttgcg ccgtcctgcc tttgccgttg ccgagaccgt aggcgatatt gcggtaaacg
    13681 gtcaggtggg ggaacagcac gccttcctgt acgaggtaac ccaaacggcg ttcgcggacg
    13741 ggaaggttgg tatttttcga gaagatggtt ttgccggaaa gcgaaatttc gccggaatcg
    13801 ggttgttcga aaccggcaag gcagcgtaaa agggtggttt tgccgcagcc ggacgcgccg
    13861 atgataaaga gaatttcgcc cgggtcgagg ctgagcgaaa tgtcgtttaa aaccggggtg
    13921 ttttgaaaac ttttggacag gtgtccgatg tgcagggcgg cggtcatagc ggtgcttcct
    13981 caagctgtta tttgaaggcg tatttcttca gcaggaatac gggtatgccg gaaaataata
    14041 ccagcatcag cgcgtaaggg gcggcggcgg cgtattgtgc gtccgatgtg tattcccaaa
    14101 cggcggtgga gagcgtgtgg acatcgtcgg cggtcagcag cagggtggcg gtcaactctt
    14161 tcatcagctt gaggaagacg agtgcgaatg cggcggtaat gccgggcagg atggacggca
    14221 gtaccaacgt cctgaaaata aagaagtgtc cgcgccccaa tgttgcgccg acctgttcca
    14281 tccctttggg gagttgttcc aaggaagtcc tcagggtggt ttgcgccatc ggcaggtaaa
    14341 gcatgaaata ggcaaggatg acgacgataa aggtttggta aacggcaggg gtgtagttga
    14401 tgctgaaata aaccaaggat agggcgataa ccaaaccggg gacggcgtgc agtaaaaacg
    14461 gcagcctgtc tatccaaacg gttaaaaaat tgcgatagcg aaccgatgcc caaacaaggg
    14521 gcaaggcaca taatatagtc aaaatcgcac ctaaagccga tacgcttaag gaacggataa
    14581 aggcatcaaa tacggatacg agcgcgaatg tgccggaagt gccgaccatc atccaatgta
    14641 tcaatacgcc aaaggggata ataatgccca aaatcaacaa gctgcttaaa aaaacaatcg
    14701 cgccgatctg accgggcagt ttgagggttt tgacgggata aggacgggca acgcctttgc
    14761 cgctgtggta aatcttggct ttgccgcgaa atatgctttc tccaaatacg acgatgccgc
    14821 acaccgccgt caaaacagcg gaaagcaggg cggcggtatt gttgttgtag gacatttcgt
    14881 attcttggaa aatggcagtg gtaaaagtgg ggtagttcaa aatggatacc gcgccaaatt
    14941 cgaccagcat atgcagggcg atcagtaaca cgctgctgcc gatggcgggt ttgagctgcg
    15001 ggaggatggc ggaaaaaaag gtttgcaggc ggcttttgcc caaggacagg ctgacttctt
    15061 cgtaagacag gctgatgcgt ttgagtgccg cctcgacggg caggtaggcg agcggaaacg
    15121 aggacaggct cataatcatc actgtccccc aaaagccttc gacgcggaag gtcaggctga
    15181 tccaggtgaa acagctgaca aatgcgggga tgcacaaagg cagggtgatt gccgtctgaa
    15241 aaaaggtttt gccgaagaag cggtaacgtt ggaacaaaag ggcgcaggca atgcccaaaa
    15301 caatggaaat cagggtaacg cccgccatca tcgtcaaggt gttggagagc aaatcccaca
    15361 tacgcgggcg gaacaacagt tcgacggcgc ggttgatgcc gacctgccac gaacgcatag
    15421 cgacatataa aaaaggcagg gtaagcggca gggcgatcag taggatgagg ccggtaagcc
    15481 aaatgggtat ttttttagga gacatagtgt ttttcatcgg caaaacgggc ggacagtata
    15541 aatgtccgcc cgtttgacaa tccgaaaacg gcttatttca taccggcttg ctcaagcagc
    15601 cgggtggcgt gttctttttc ggaaacagtg gtggcggaca cttggggtgc ttccaacttg
    15661 gcgatgggtt ccaaattgaa ggtggatacc acgtgcggat tcaaaggata ttcggcacgg
    15721 acggcggtca gggcgcgctg tccttccttg ccggcgagga aggcgacgaa ttttttcgcc
    15781 tcatccttgt tttgggagga ttttaacacg gctgcgccgg aataggtaac gagtgcgccg
    15841 ggatctctgt ggcggacgaa attcaggcgg gtgtggacat tttgtacgcc tttttcacgc
    15901 gcgaaagcgt gccagtagta gttgttgatg agggcggcat cgatttcgcc gttttcaacc
    15961 gcttgaaggg cgacggagtt tttagcgtaa ggcttgccgt attctttcag gcctttgagc
    16021 catttcaatg cggccgcttc gcctttcagt ttgacgatgg cgacaatctg ttccaagaac
    16081 gcgccggaag tggggacgta accgatgcgg tttttccatt tcggcgtggc gtaattcagg
    16141 acggattttt ccaaatcttt ttcagacagt ttgcgggtgt cgtaaacgac gacgcgcgaa
    16201 cgtccgctca gtgccaccca gtcttttttg gcggcaaccg gcacgccttt gccgcgtgtt
    16261 tcgttgatgg tggaggcggg caggggctct aggaggttgg ctgcggaaag ggtggcgagt
    16321 gccgggattt gttcggaata gaatacgtcg gcggggcttc ggctgccttc ttctttgatt
    16381 tggccggcaa gctggtcgcc tttggcactg ttgagtttga ctttgatgcc ggtagcccgg
    16441 gtaaaggcat ctgcaacggc ttgtgccgct tctttgtgtt ggccgttgta cacggtaatg
    16501 tctgccagcg cgggggtggt ggcggtcagg gctgcggcaa gcagtgcgta tcggatagat
    16561 gttttcatat cgattttctc ctaaatgaat gagggtgtat accttgttaa gacataacgg
    16621 ggtgtagtgt attccttctt ttttataaat gcaaataatt attttttaaa tttgttatta
    16681 tccgatccgg ttattgtttg ttctgacttg tattttttcc gtgcatcgcg cccgtaaggc
    16741 ggaagcggcg ggcaatgcgt ggcggaatgt gggtaaaggc ggcattttga tttgtcggaa
    16801 tgctggagaa cctctccctt taaaacgccg tctgaacaag gttgccggaa tagtattgcc
    16861 atcccggcag atacagtttg tcgggatctt gccaatattg ttcatccaga ctgttcggca
    16921 gcgaggcggt tttgttgtcg agatgttttg ttaatccact atatatccca catttctttt
    16981 aggtttttac cttccgattg gaggtggcgg atttcttttc ccaatgcgtc tgccgcccga
    17041 tggatcagca ttgcgctgtg taccgcgccg atttttttca gaacggaaca agtcttttcg
    17101 gcggcgggaa aaccccagtt gcagacaaat tgcagtatgc cgccgttgcc gatgtcggct
    17161 tccgcccgcc aaatcaaaac cagctcctgt tcccgtccgt ccatgctttc gagtttgccg
    17221 tcatgctgtt caaaaagttt gtccaccgcc tgccgcatca tctgctcgaa gcggtcggct
    17281 tgggtatccg tatcggtcat aatattcttg ccttttaaaa atgccgtctg aacatttctt
    17341 cagacggcat ttgggggtta agctgacatt tcccgccagc gtttgacttg gaggcgtacc
    17401 tgttcgggcg cggtgccgcc caagtggttg cgcgcgttga ggctgccttc gggtgtcaga
    17461 acgccgtaca cgtcgtctga aatcagcttg ctgaagcctt gcagggcttc gagcggcaat
    17521 tcgctcaaat caacgccggc ttcatcggcg tggcgcacgg cttgggcaac gacttcgtgg
    17581 gcatcgcgga agggcatacc ttttttgacc agataatccg ccaagtcggt ggcggtggca
    17641 aaaccctgca tcacggcggc gcgcatattg ccgggtttga cggttacgcc gcgcatcata
    17701 tcggcgtaaa tccgcagcgt gtcgatgagc gtgtcggcgg tgtcaaacag cggttctttg
    17761 tcttcctgat tgtctttgtt gtatgccaag ggctgcgatt tcatcagggt aatcaggccg
    17821 ataaggtgtc cgatgactcg gccggattta ccgcgcacga gttcgggtac gtcggggttt
    17881 ttcttttgcg gcatgatgga cgaacccgta cagaagcggt cggcgatgtc gataaagccg
    17941 aaacgcggac tcatccacaa aatcaattct tcagacaggc ggctcaagtg aaccataacc
    18001 agcgaggcgg cggcggtgaa ctcaatggca aaatcgcggt cggatacggc atcgagcgag
    18061 ttctggcaga tttgttcgaa acccaacaat tcggcggtga tttcgcgctg aatcggatag
    18121 gtcgtaccgg caagggcggc agcgccgagc ggcatacggt tgacgcgttt gcgacagtcg
    18181 gccatgcgtt cgaaatcgcg tccgagcatt tcgacgtagg cgagcatgtg gtgtccgaag
    18241 ctgaccggct gggcgacttg cagatgggta aaaccgggca tgacggtttc ggcgttttgt
    18301 tccgccaaat ccaataatgc cgtctgaagg ttttgaatca ggttttggat aacggtaatc
    18361 tggtcgcgca gccaaaggcg gatgtcggtg gcgacttggt cgttgcggct gcggccggtg
    18421 tgcaggcgtt tgcccgcgtc gccgattttg tcggtcaggc ggcgttcgat gttcatgtgg
    18481 acatcttcca aatcgagcgg ccattcgatt ttgccgctgc ggatttcttc gaggatttcc
    18541 gacatgcccc gacggatgtc ggtcagatcg ttttcactca gcacacccga ccgggtcagc
    18601 atctgcgcgt gggcgagcga gccttggatg tcccattcgg caaggcgttg gtcgaaaccg
    18661 atggaggcgg tgtattgttt gacgagttcg gaaacgggtt cgttgaaacg tccggaccag
    18721 gttttgtcgt gcataaggat tccttgatgg ggttattcgg tgcggtattt ttccaaaagg
    18781 cggcggaagg gttcgccggt ttcggggtgt tttaaaccgt aggcgacggt ggcttcgaga
    18841 tagccgagtt tgctgccgca gtcgtagcgc gtgccttcaa aggggtgcgc caagacaaat
    18901 tcgtgatcga gcagcttggc gatgccgtct gtaagctgga tttcgttgcc cgcgccgcgc
    18961 ggcaggccgg ttaaaaggtc gaaaatgcgc ggggtgagga tgtagcgtcc gacaacggca
    19021 aggttggagg gcgcgtcttc gggcttgggt ttttcgacaa tgccggtaat gcgttggaac
    19081 tgtttgagct gttcggtttc gacgatgccg tatgagccgg tttgcgacgg ttcgacggtt
    19141 tctacgccca aaatgctgtt accgctgcgc ccgtacactt cgaccatttg tttgagcgcg
    19201 cctttgggcg catcgattaa gtcgtcggca aggatgacgg cgaagggttc gtcgccgatg
    19261 gcggcgcggg cgcacaagac ggcgtgtccc aatcccagtg cttccgcctg acggatgtag
    19321 aggcaggtaa tgttcggcgg caggatgttg cggacgtgtt ccaacaattt gtctttatgg
    19381 cgcatttcca acttagtttc gagttcgtat gccttgtcga aatggtcttc gatgctgcgc
    19441 ttgttgcgcc cggtaacaaa caccatttcc gtgcagccgg cttccacggc ttcttctacg
    19501 gcgtattgga tcagcggctt gtcgacgatg ggcagcattt ctttcgggtt ggccttggtg
    19561 gcgggcagga agcgggttcc catccctgca acggggaaga cggctttttt tatcggtttc
    19621 attctttttc ctttgtattg ttttgatgtt taaagggcga gtttgcgaat taaatcggcg
    19681 agtgcctgcg cgcggtggct ttcgcggttt ttgacctccg aatccaattc ggcggcggtt
    19741 ttgccgtgtt cgggcagata aaaatacggg tcgtaaccga aaccgttttg cccgagcggc
    19801 gtgtcgtgcc attgtccgtg ccatatgccc tcggcgataa tcgggcgcgg gtcgtcttta
    19861 tgacggacaa aaaccaatac gcagacataa gaacaacttt tgtcggcctt gccggcaagt
    19921 tcggcggcaa gtttcaggtt gttggcggta tcggatttgg gattgctgcc ggcgtaacgt
    19981 gcggaatgga tgcccggcgc gccgtttagg gcggcggcac agatgccgct gtcgtcggcg
    20041 agtgcgggca gcccgctgtg tccggcggcg tgccgggctt ttgccagcgc gttttcgaca
    20101 aaggtgggat agggttcggg gcattcgggt atgccgaatg cggattgcgg caatacggtg
    20161 atgccgtaag gtttgaataa gttgccgaat tctttgagct tgccggcatt gccgcttgcc
    20221 aaaacgattt tttccggttt ttcagacata gcggtttccc tgtgatgaag atggggcggc
    20281 gcgtagggat ttgtgccgca ggtagagggc gagtgcgccg atttgcccga acagcgcgcc
    20341 gaatgcgaaa aggaaggacg cgacggcgaa ggctttgctg ccggcggtca gcagataagc
    20401 acccaagagg acgggcagca taaatgccag tgtgaacaag gcaacagaca acagatagat
    20461 ttttcggcgg ttcatggcgt tcggtcggaa acgggatgtt cggattatag ccgattagga
    20521 cggcattccc tagaggctgg aaaaatgccg tctgaagcgg cgttcagacg gcatggcggg
    20581 cattatgcct gtttgttcca acgttcgatg gattctttga tgactttttt cgcttcttcc
    20641 gcaccgcccc aggattcgac tttggtcgta cccgcttttt tcaggtcttt gtagtggttg
    20701 aagtggaact cgatttgttt gatgagctgt tgcggcaaat cggacaaagt tttgtaggcg
    20761 ttgccgttat tgcggtcgtc ggcaggtacg cagacgattt tgtcgtccac ttcgccgtcg
    20821 tcgacgaatt tcatcacgcc gataacgcgc gcttccaaga atacgccggt tgccagaggt
    20881 tgttcggtaa cgagcaacac gtccaattca tcgccgtctt cgtccaaagt ttgggggatg
    20941 aagccgtagt tggtcggttt ggcgaagatg gcgggttcga tgcggtcgag ttggaatgcg
    21001 gcgagtttgc ggttccactc gattttgtgg ttgctgccgg cggggatttc gttgacgacg
    21061 ttgatgatgc cgccgtccac gtcgcccggg gtcaggattt ggttgaagtc tgccatttgg
    21121 tttcctttat ttgggaaggt ttgaagtttg aaagtatagc acaaacgtcc ggctggaaat
    21181 gcgtccgatg ccgtctgaaa gggaaaccgg gcgcgtgtta ccgtttgccc aaaaacctgc
    21241 ccagttccaa aatcgcgcgc ctgttggacg gggaaaacac tttttccgcc gcttcttcca
    21301 agccgaacca gccgtaggag acgtgttcct cgggttgcag gacgacgggc gtatcgcgcg
    21361 ggatttcggc ccggaagacg tgttcgcggt tttcaaacac gcctttgggg tagcggtgcc
    21421 gccagtggtg gtagatttcg taaaccgtgc tgtcgtgccg gtcttggagc tgcccgtcct
    21481 ccagcaggat gccggtttct tcccaaactt cgcgccttgc cgtttgggcg acggtttcgc
    21541 ccggttcgag gctgccggtt accgactgcc aaaatccttt cggatgcgtg cgttcgatga
    21601 gcaggatgcc gccgtccccg ctataaagga cgaccagtgc ggaaacgggg tatttgagcg
    21661 gttttgccat cggcatcttt cggcgggctg cggtaatgaa ggggcggatt atagcaaacg
    21721 ccgcacgtta tggcgtttat ccttttccgt atcctttttt ctgcacggat gggacgcgcc
    21781 ggtgtttgcc ggtaaatttt cctttgtgtc aaaaagataa gggcggttgt gattttaatg
    21841 cttgccaaag tgacgggcgg aaactataat ccgcgactta ccgagtcgga gtgtggcgca
    21901 gtctggtagc gcacttgcat ggggtgcaag gggtcgaagg ttcgaatcct ttcactccga
    21961 ccaaaaattc cgaaagccgc tttcaaaagc ggcttttttg ccgtccgtat gattatgatg
    22021 tagagtacgc ggcgacagac attcaaatgc cgtccgaaaa ccgttcagac ggcatctctt
    22081 tatctt
//
LOCUS       DAXDZG010000033        20684 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXDZG010000033 DAXDZG010000000
VERSION     DAXDZG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20684
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.033"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(116..667)
                     /locus_tag="ACFB78_001073"
     CDS             complement(116..667)
                     /locus_tag="ACFB78_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269074.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
     gene            905..2377
                     /gene="pyk"
                     /locus_tag="ACFB78_001074"
     CDS             905..2377
                     /gene="pyk"
                     /locus_tag="ACFB78_001074"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC1269075.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2611..3963
                     /locus_tag="ACFB78_001075"
                     /pseudo
     CDS             2611..3963
                     /locus_tag="ACFB78_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(4046..4468)
                     /locus_tag="ACFB78_001076"
     CDS             complement(4046..4468)
                     /locus_tag="ACFB78_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269076.1"
                     /translation="MAGCLTDLENCLEKYLEQFGPVSESIEACTAKLQEQPSFFNRLM
                     KANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHNRKADYAEKGKERIAL
                     FPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(4651..4800)
                     /locus_tag="ACFB78_001077"
     CDS             complement(4651..4800)
                     /locus_tag="ACFB78_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269077.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(4818..5015)
                     /locus_tag="ACFB78_001078"
     CDS             complement(4818..5015)
                     /locus_tag="ACFB78_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269078.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(5025..6041)
                     /locus_tag="ACFB78_001079"
     CDS             complement(5025..6041)
                     /locus_tag="ACFB78_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC1269079.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(6140..7354)
                     /gene="gltS"
                     /locus_tag="ACFB78_001080"
     CDS             complement(6140..7354)
                     /gene="gltS"
                     /locus_tag="ACFB78_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC1269080.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(7607..7888)
                     /locus_tag="ACFB78_001081"
     CDS             complement(7607..7888)
                     /locus_tag="ACFB78_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269081.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(7907..9031)
                     /locus_tag="ACFB78_001082"
     CDS             complement(7907..9031)
                     /locus_tag="ACFB78_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1269082.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(9094..10101)
                     /locus_tag="ACFB78_001083"
     CDS             complement(9094..10101)
                     /locus_tag="ACFB78_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC1269083.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(10094..11095)
                     /locus_tag="ACFB78_001084"
     CDS             complement(10094..11095)
                     /locus_tag="ACFB78_001084"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1269084.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGRTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(11126..13399)
                     /locus_tag="ACFB78_001085"
     CDS             complement(11126..13399)
                     /locus_tag="ACFB78_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC1269085.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            13514..14530
                     /gene="galE"
                     /locus_tag="ACFB78_001086"
     CDS             13514..14530
                     /gene="galE"
                     /locus_tag="ACFB78_001086"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC1269086.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            14531..15571
                     /gene="rfbB"
                     /locus_tag="ACFB78_001087"
     CDS             14531..15571
                     /gene="rfbB"
                     /locus_tag="ACFB78_001087"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC1269087.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            15676..16542
                     /gene="rfbA"
                     /locus_tag="ACFB78_001088"
     CDS             15676..16542
                     /gene="rfbA"
                     /locus_tag="ACFB78_001088"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC1269088.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            16681..17238
                     /gene="rfbC"
                     /locus_tag="ACFB78_001089"
     CDS             16681..17238
                     /gene="rfbC"
                     /locus_tag="ACFB78_001089"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC1269089.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(17282..19300)
                     /locus_tag="ACFB78_001090"
     CDS             complement(17282..19300)
                     /locus_tag="ACFB78_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC1269090.1"
                     /translation="MNKSLSGSVEKYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLVLLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(19495..20616)
                     /gene="dnaJ"
                     /locus_tag="ACFB78_001091"
     CDS             complement(19495..20616)
                     /gene="dnaJ"
                     /locus_tag="ACFB78_001091"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC1269091.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         5135 a         5772 c         5017 g         4760 t
ORIGIN      
        1 ggtttttgtt aatccgctat aaaagcaaag aaaccgccat tagcactaca cccccccaag
       61 ctgcggctgc caaacacatc ggctgcttct cgacgcaccc gctgaaaaat gcgccctatc
      121 cgcccgcctt gaccgcaccc cctgcctttg ccaccctgcc cgatgccgtc tgaagcctga
      181 accgccctac cagcccgatg atgcacgaca ccgccggaaa gggcatacac aaagccgtca
      241 gcacataacg cgcattcccc gtattgacca cgtccgcgcc cttcagcagc agcggcagcg
      301 cgttcgccca ctcccacagc gaaggcgcgc tgaacgccaa taccgccacc agccccaaca
      361 tccggaacac cccgatttcc aagacctgcc gccgccgcac cgaacggttg agcaaaaaca
      421 caaccgccaa cccgaaacag accaccgccg ccataccgcc gttggcgaca atctgcaaac
      481 tgttgaacgc aatatcgggc gtaaccgcca acacgccttc cgaatccggc acggcacgct
      541 gcaaatccaa accctgcaac acattcaaca caaaaccgta gatcaaatca aaaaacacca
      601 cgcctatcgg caaagcactc aaaacccgcc acatcttcct gtccgaccaa ccgttcaaaa
      661 ccttcatttt acccgaccgc gcaagccgcc gaacaaaaaa taaatttagc ctaaatcata
      721 aaaccgtgta attttgcacc aaaaaaggcg gtttgcaggt aaaatatcaa accattaatt
      781 taaatcatat ttttacagaa tattccgccg cgttcaccaa atggacatca aaccggttcc
      841 aaatttctgt aattttgcaa caaaataccg caaaacaccc gattgagacc gaaaggactt
      901 tcatatgaac cagacaagcc gcgatctgac ccgcatcagc cacaacacca aaatcgtcgc
      961 cacccttggg ccgggcagca acaacgtcga actgttggaa gacatgatcc gcgtcggcgg
     1021 cctgaacgtc gtccgcttca acttcagcca cggcacgccc gaattccatc aggaaaacgc
     1081 ccgcatcgtg cgcgaggcgg caaaacgcgc cggacaggaa atcgccatca ttgccgatct
     1141 gcagggcccg aaaatccgcg tgggcaaaat cgccggcggc ggcatcgaat tgaacaaagg
     1201 cgaaacattg gtactcgatg ccgcgctcga aggcgaaggc acgcgcgagg cggtcggttt
     1261 ggactaccgc gacctgcccg acgacgttgc cgcaggcgat gtcttgtggc tggacgacgg
     1321 cctgctgacc ctgaccgtgg aatccgtcga aggcagcagg attatcacaa gggtggaaaa
     1381 cagccacatc ctgaaaagca acaaaggcat caacaaacgc ggcggcggtt tgtccgcagg
     1441 cgcgttgacc gaaaaagact tccgcgacct gaaaaccgcg attgccatcg gttgcgacta
     1501 tctcgccatc agctttgtga aatccgccga agacctgcac atcgcgcgcg ccaaagtcga
     1561 agaggaaatg aagggcagca ctgccgtccg ccccggtttg gtttccaaaa tcgaacgcgt
     1621 agaagcgatt gaaaaccttg atgaaatcat ccttgccggc gacggcatta tggttgcgcg
     1681 cggcgacttg gcggtcgaag tcggacacgc cgccgtcccc gccctgcaaa aacggatgat
     1741 ccgccgcgcc cgcgagttgc gccgcttcag cattacggcg acgcaaatga tggaatcgat
     1801 gattaccaac cccgtgccga cccgcgcgga agtcagcgat gtggcaaacg cggtattgga
     1861 cggcaccgat gcggtgatgt gttccgccga aaccgccgtc ggcgcgtatc cgtttgaaac
     1921 cgtcagccaa atggcgatta tctgcgcggc tgcggaaaaa gaacaggatt cgctcaacgg
     1981 cgttgccgaa cagacagagt atcccgaagc cgtcagcacc aacttagcga ttgccggcgg
     2041 cgcggtcagc gtggcgcgcg cggttcacgc caaagccatc gtcgccctga ccgaaagcgg
     2101 ttcgaccgcc tttgaaatca gccgccacaa tattaccctg ccgattttcg cgctgacccc
     2161 gagcgtttcc gcccaacgcc gtatggcgat gtaccggggc gtgcgcccgc tgattttggc
     2221 aaccagcacc gaccacgaca cggcgttgaa cgaagtcgaa acgatgctgg tggaacacaa
     2281 catcctgcat tccggcgacc agtacatcat caccagcggc tcgcaaatgc gcgaatccgg
     2341 ttcgaccaat acgctggaag tgctgcgcgt caaataatcc gccctgagtg gaaaatgccg
     2401 tctaaagccg atactgtcgg gcttcagacg gcattttatt tgcattccgt caggtaaaac
     2461 cgaaagaatt gtgcaaatcc ggcaacatcg gataaaatcg agtgcctata ctaaagcgaa
     2521 acaaggcatt tccgactgcc ttttttattt gtccaccgtc cgccttttta cggaaaccga
     2581 aatgacccct ttcacactga aaaaaccgtc ctgctgctcg gcactgcctt tgccgccgca
     2641 tctgtccacg catccggcta ccacttcggc acacagtcgg tcaacgcgca aagcacggca
     2701 aatgccgccg acgcgtcgac catcttctac aatcccgccg gcctgaccaa actcgacagc
     2761 agccagattt ccgtcaacgc caacatcgtg ctgcccagca ttcattatga agcagattcc
     2821 gccaccgact ttaccgggct tcccgtccaa ggttctaaaa acggcaaaat caccaaaacc
     2881 acggtcgcac cccacattta cggcgcatac aaagtcaacg acaatctgac cgtgggcttg
     2941 ggcgtgtacg tccccttcgg ctctgccacc gaatacgaaa aagattccgt gttgcgccac
     3001 aacatcaaca aactcggtct gaccagcatc gccgtcgaac ctgtcgccgc gtggaaactc
     3061 aacgaacgcc attccttcgg cgcaggcatc atcgcccaac ataattccgc cgaactgcgc
     3121 aaatatgccg actgaggaat cccaaaaaaa gcgcaaatgc tgcaagcaac accttctaat
     3181 cctactgccg ctgctcaaat caaggccgac ggacacgccg atgtcaaagg cagcgattgg
     3241 ggcgtcggct accaactggc gtggatgtgg gacatcaacg accgcgcgcg cgtgggcgtg
     3301 aactaccgtt ccaaagtttc acacacgctc aaaggcgatg ccgaatgggc ggcagacggc
     3361 gcggcggcga aacaacagtg gaatgacaat atgctcacac cgctcggtta cacggcgaat
     3421 gaaaaagcca gtgtcaaaat cgtaacgcct gagtctttgt ccgtacacgg catgtacaaa
     3481 gtgtccgaca aagccgacct gttcggcgac gtaacttgga cgcgccacag ccgcttcaat
     3541 aaggcggaac tgttttttga aaaagaaaaa aatattgcta atggcaaaaa atccgaccgc
     3601 accaccatca cccccaactg gcgcaacacc tacaaagtcg gcttgggcgg ttcttatcaa
     3661 atcagcgaac cgctgcaact gcgcgtcggc atcgcttttg acaaaccgcc tgtccgcaac
     3721 gccgactacc gcatgaacag cctgcccgac ggcaaccgca tctggttctc cgccggcatg
     3781 aaataccata tcggcaaaaa ccacgtcgtc gatgccgcct acacccacat ccacatcaac
     3841 gacaccagct accgcacggc gaaggcaagc ggcaacgatg tggacagcaa aggtgcgtct
     3901 tgcgcacgtt tcaaaaacca cgccgacatc atcgacctgc aatacaccta caaattcaaa
     3961 taaacgttac accgtttgaa tataaaaatg ccgtctgaaa cagatttccg tttcagacgg
     4021 catttttatc cggcagccga tacgcctatc tcggtattcg ggagaatata tacagaacct
     4081 ccttcatatc aaaatggtag ggacgctcgg gcaacaggac ggcggcaggc agcttggtga
     4141 tgccgttcaa accgcgcggg aacagcgcaa tccgctcttt gcccttttcc gcataatctg
     4201 ctttgcggtt gtggatggaa actacttcgc gatggcggta aagcagcgtg ttcatttcga
     4261 tataggcttc gttgtggatg gctgccgact gtttctgcaa cacgtcaatc tgcctgttga
     4321 gcttgtcgtt ggctttcatc agcctgttga agaacgacgg ttgctcctgc agtttggcgg
     4381 tacaggcttc gatactttcc gataccggcc cgaactgttc gaggtatttt tccagacagt
     4441 tttccaagtc ggtcaggcag ccggccatct tgcgggaaaa ttcttcttca ggctttccct
     4501 gccgaatgga caatatagcg gattaacaaa aatcaggaca aggcgacaaa gccgcagaca
     4561 gtacaaatag tacggcaagg caaggcgagg caacgccgta ccggtttttg ttaatccact
     4621 atatttggga ggggcgtaac cccttccgaa tcaggatggc acatagggcg acgctttatg
     4681 tgccgtcctg tgtgttgaaa catcagcata ggaaacacag gaaaagccgg ctgttttgcg
     4741 cctgcgtacc gtattggtca aaatgccgtc tgaaaccgac aacagggttt cagacggcat
     4801 ttccgtcaat tttcccttta tctgccgaac aggttggcga tattttccat ctgttcttcg
     4861 gtaaaaccgt tttcgcccaa tctggcggca actgcccatt cgcccgacaa accgtggtcc
     4921 gcaccgagct gccgccattt ttcgactttg gcttcggctg tggttttttc gggcagcggt
     4981 ttgacgggaa tcctgctttt ggggacggta ttcatcggtt tgccctaatc gatggaatag
     5041 ccgcgaagat actgtatccg ttcgcgcgtc atccgcgtgt cgcattggag cttgaggtat
     5101 tcggcgtatt cctgccggtc tgcctgcgcg gcggcttgtc ggcagttgct gattttttcc
     5161 tgcgcccact tgcgctgttc gccgaccaac tctttttgca cgtcggtatc gagtcctccc
     5221 caaagtttgg taatttcgga ttccgcacgt tggttttgta cgcgcgcctc ttccacttcg
     5281 ccccgtgata cggtaacggt atcggcacgc tcgacgtcgt cgggatgcag gatttcgggt
     5341 tcgggcgcgc cttctgcggc ttggggtacg cccgcatcgc cgccggcggc attgtgttcc
     5401 aaaatgtctt cgggggtggg tttggacggt tcttcttcac gggctttgcc gctcaaaacc
     5461 ctgaccgcgt cttcttttgt caccgccttg ccgtctatca tcacgatgct cttcacgccg
     5521 taaggcagca acgcggcaga cagcgtttgc gtcgccatac cgaccgtgtt gtcgataaat
     5581 gccgtccgag cgtctttggc gggcaggaag cggacggctg ccgtcaatac gccgtcttta
     5641 aactcgacat tgccgcccgt cttctgctgc acgatgtctg ccaaagacgt ttccccatac
     5701 agcagggggc tgtttgcctc ggcatcggca agcgtttcag acggcacggt aatgttcaaa
     5761 tcggcgatac agaacgtgcg cccgccttcc tgcgtttccg aagcgtgttc caaagaaaac
     5821 gccaaaccgt aggcggcggc gataattttg tcggcatcga caaactgcct gccgtcttcg
     5881 cgcgcgaaag aacgcgcttc ctgcgtgagc gtttcctgaa tactgccgcg tatgtcctgc
     5941 aacacggcgg ggttggcgca ttccaacgcc ttgggcggtt cttccctgcc gcacgctgca
     6001 agcagcaggg caaacggcag cgcaatgagt ttccgataca tgatgttcct tccaaacggt
     6061 gcagaccgat ggcggctatc atagcaaaag cacctgcagg cggtaaacct tgcaggcgct
     6121 ttgtcggaaa ggcggaaaat cagcctttaa agaaattcac aaaaccggtg agaatcgcgg
     6181 cattaatcaa atcgacaaag aacgcgccga ccataggcac aatcaaaaac gccttatgcg
     6241 acgcgccgaa agtatgcgtg acggactgca tatttgccac cgccgtcggc gttgcgccca
     6301 aaccgaaacc gcagtggccg gcagccaata ctgcggcatc atagtcgcgc cccataaaga
     6361 cataggtaac aaaagtcgcg tacaaaacca tcactgcggt ttgtactgca agaatcacgg
     6421 ttacagaccc cgccaaaccg gtcagctccc acagtttcaa attcagcaac gccattgcca
     6481 agaaaagcga aagcgaagca ttgccgaaca catcgatggc acggtcgaac atattgacct
     6541 tgaatgcggc ggtaaggatg ttgcggataa ccacgccgcc aaacagacac cacacgaatt
     6601 tgggcaggtc gaacaggtat tctttgtcga agccgtccat aatctcggca aacgccagac
     6661 acgcggcaaa catggcaagc gtttcaacgg cagattccgc cgtaatcagg cgggtgcgtt
     6721 ttgcctgctc gaacacgtcg tccgcgttgt cgtcctgatc ctgttttgtg ttttcaaccg
     6781 gtttgcggcc cattttgttg atcaggcggc gcgcaaccgg cccgccgatc aggccgccga
     6841 acaccagccc gaaagtagcc gaagcaatac ccaaaccggt tgcgccgacc aagccgtatt
     6901 gcgtttcaaa attaggtccc cacgcacctg acgtgccgtg tccgcccgtc agcgacaccg
     6961 aaccggtaat cagaccgatg agcgggtcca aacccaaagc cgtagccagt ccgaccccga
     7021 caaagttttg caccaagata aatccgccca caatcgcggt aaaaaccacc agcggcaaac
     7081 cgcccgcctt caaacgggaa aaatccgcgc tcaagccgat ggacgtgaag aaaatcagca
     7141 taaacgcatt ttgcagcggt ttctcaaatt tgaagctcac gccgtacgcc tcgtgcagcg
     7201 caaacaggat aatcgcggca atcagcccgc ccgccacggg ttcggggatg ttaaaatcac
     7261 gcaagatttt gattttctta accaaaacct tgccgaccaa caaaaccaaa gtggcggcaa
     7321 tcaaggtgta ataactgtta aacgcccatt ccatatcgtt tgacctccta aaatgttttt
     7381 ctttatatcc gcccgaaggg gacgcaagcg cgaagatatt aggggaatag ttaaggcttg
     7441 tcaaagaatc gccgtttttt atagtggatt aaatttaaac cagtacatcg ttgactcgcc
     7501 ttgccgtact atctgtactg tctgtggctt cgtcgccttg tcctgattta aatttaatcc
     7561 actatacaac tattttttac aaacaaaatg ccgtccgaaa ctccgttcag acggcatcat
     7621 gggaatacac atatttgaaa taacagaaaa agcgggggca aaacccgccg ctgcatctgt
     7681 cgctgtacat ccctgctgtc ggacttaccg cttcaacttc gcaaacgcat ccgccatcgc
     7741 cgaattggtt gggacacgct cgttgcgttg tggtttgcgg ccggctgtcc gttcgcggct
     7801 gcggtttttc cagctcggac agaatgtcca gaatggtcgg cagtccgaag tgttcgtcgg
     7861 tgaagtcgga cgctttgatt tgcttcacgc gctcgcggtt aactgcctaa atatgaaatt
     7921 cttggtcttc ttgtagcata agttctcgat gatatgccaa gtaagattta cgtttgtcgt
     7981 tgagcagatt gttatctagc ttcacatcac accaacgtcg ccaaacatca tctgataacc
     8041 gcttagattt caccatattt ccttcatcat caaacgaaat atatttcaaa tcaaataggc
     8101 tatccaaggt acgacttaat agcaagccgt tatttgggtc gtatgcttcg gtatcatccg
     8161 acaggatgaa aggcttaata tgactggcaa tcaatacagg ataggcaagt ttttccagca
     8221 tacatttcac attaccgtaa tgttcttcac tttcttcttg aagttggttt ttgtataggc
     8281 gatgtaaata agggtcgcgt ttccttacag taatttcatc caaattacca aaaatacgtt
     8341 gggcatcctc agcaaaatat aagtcatcac ctactcgttt taagtcgtct aatttgtcta
     8401 ataggttcca taaataactt atttgattgt attttcgttc aataaatcct gattctattc
     8461 cctgttgaaa ataatcattc aattctgttt cggttagata atcttgttga aaggttttta
     8521 agtctactaa catcatggcg gcaatttctt ttttatttag cttgccttgt ggatgctcga
     8581 ccagcgtttt aatcaggaaa tttatttgtc gaatattaga gttttcagtt actgaacgct
     8641 gaaagccaga gtgcgtatat acgattttgg aaagtagggt ttgtcgtttt cggttagtac
     8701 gagcttgtac ataatctcgt gataaaggtg tatagcttga taaaaacggt tctataaatc
     8761 ccatttttac aaattgatta atagctttac gaaccgaggc caaattcatg gccttgcttg
     8821 ttctctgcga agccataacg gaatcttggg caatgttttg ctgcaatgct tcatatagtt
     8881 cgttgctgta tggctggtct tgattcgcat caataaagtc aagacaaata ttgagtgcag
     8941 tacaaaattt ttgatcgtta taatttgtaa aggcattggt aaacgcccaa taatcttcat
     9001 aaatttggct gtttggctga ttattactca tctctgtctc caagaatatt cgataccaat
     9061 attctgtttt tgtatttaag taatatctat cattcatgta ttttctacta aaaacagaat
     9121 ttccttatgg ctttgcttct tttcttttcc tgtaacctta taaacgtgag gcgtttccaa
     9181 gatttgtaca tttcgcccat ttttacctaa catttcttct aattcatctc ggtttggata
     9241 tgaagcattg ttgtagctta acagccaata tttaaatggt ttcaaatgat tgattaccct
     9301 tttaaataat tcgacagcct gctttttatc catgaaatga ttggcaaatg gtttgggtat
     9361 ggatgatgta atgtaactgt ccaataatcc gtaaaagcca aaataattgt tcatcgtgcc
     9421 tgtgtaaggt gggtctaaat agacggcatc agcttgtata ttaggtaata aatcaaatat
     9481 atcttgatta aaggcgtgat gccgccagcc attattgaaa acagcttggt tatagtcatc
     9541 caaattttgc agaaaatgac tttgaaaact ttcattgtgg taggcgcgtc gacggccata
     9601 tttagcataa ctgtattctt catcacgcag ttgtttaact ttttcccaag gaattgtgaa
     9661 tcgtgaatag ggcattttac gtatcatggc gcgacgcatt aaagtaaacg ccaaagcacg
     9721 tttatactgg ttattcagct tccctatatt tttacggtac aaatcaagtt gttggcattc
     9781 atcgtgaaaa tagaattttt cggaataacg ctgactcata aaaccggcat gcgggctgcc
     9841 tgaaaaaatc attgcgacat catcgtcatt taatgtttca tggttgtttt ctattaatgc
     9901 taaagcaatt tggtaattaa ttgccaaaat atcgttagta atcacacgat agccgcgttt
     9961 tttggcttcg taggcaaagg aacagcctcc actaaataca tctgcaactg tatctacatc
    10021 agacggaagc tggtcacaaa tccaggaagc tattttctct ttattaccga tatagttaat
    10081 tttcggatat ttcttattca ttttcactca tttttaaaat actttcagca atagcttttg
    10141 ccaataaagg cggcacggcg ttaccaacct gctgctgctg ggcaattttg ctgccgcaaa
    10201 aaataaaatt atcagggaaa gattgtaagg cagctaattc acgaacggtt aaagcccgat
    10261 tctgttcata gtgaaaaact ttgcgcatat ctcctgtaat acaaacggct ggtttgttgc
    10321 tgttgtaacg gatgtattta cggatatcac ctgttttcgg acgtaatggt tcaggaatat
    10381 cgttacggtt acctccattt ttaacaaatg ccattttttc taacatttgt gccgaatgat
    10441 tcatagcttc atgatttgca acgtgtggat tgctttcgcc agcagccagt tttggaaaat
    10501 gtcctattgc tgatccaaca gtctgatggg aaatctgcaa aggttcggga aaggaaattt
    10561 tgcctttatc cctcctcccg ataaatatca ctcggctacg tatctgaggg acaccgaaat
    10621 cggctgcact cagtatctta cattccaccg aataaccgat attctgaaat gcttgaataa
    10681 tctcaatacg tgttctacct gaattgtgtg tatagagtcg cgctacattt tccataacaa
    10741 aaaaatatgg ttggacaatt ttaactattc ggacaaactc tttaaataaa tggttgcgtg
    10801 ggtcatctgt aaatgtccgt ccaatctttc ctgccatact aaaaccttga caaggtggtc
    10861 ctccaataac caaatcaact gattgtccgt taagacaatt ggttaaatct tgttcggtta
    10921 gtgtggttaa atctttttgc agtaattgat gacgggggaa gttggtacgg taagtctgac
    10981 aataatcaga ctccatttca acagaaagca attgttggaa tccggcttgt tcaaaaccca
    11041 aggataggcc tcctgctcct gagaaaaggt caatataggt aagtggtgtg tgcataagtc
    11101 aaaatccata aactcttcga ttattttacc gcttcagctt cgcaaacgca tccgccatcg
    11161 ccgaattggt cggggcgcgg tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc
    11221 tgcggttttc agacggcatt ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg
    11281 tcagcgcgat gcgtttgcgt gcggcatcga cttccagcac tttcactttt accacgtcgc
    11341 cggctttcac cacgtcgcgc gggtcttgga cgaacttatt agacagggcg gagatgtgca
    11401 ccaagccgtc ctgatggacg ccgatgtcca cgaacgcgcc gaagttggcg acgttggaga
    11461 ccacgccctc gagtatcatg ccgacttgca agtcgctgat ttcgtggatg ccttcggcaa
    11521 acgatgccgt ctgaaacgca ccgcgcgggt cgcggccggg tttttccagc tcggacagga
    11581 tgtccaaaat agtcggcaga ccgaagcgtt cgtcggtgaa gtcggatgct ttgatttgct
    11641 tcacacgctc gcggttgccg atgagttcgg cggcggtaat gccttgttgc gccagcattt
    11701 tggcgacgac gggataggct tcggggtgga cggcgctcgc gtccagcggc tctttgccgc
    11761 cgttaatccg caaaaagcct gctgcttgct cgaaggtttt ttcgcccaag cgcggtactt
    11821 ttagcaattt ttttcggctg tcgaacgcgc cgttttcatc acggtaggcg acgatgtttt
    11881 gggcaagggt ttgattcaag ccggaaatcc gcgccaagag cggggcggag gcggtgttcg
    11941 cgtccacgcc gacggcgttc acgcagtctt cgaccaccgc gtccagcgat ttggcgagcc
    12001 ggctttggtt cacgtcgtgc tgatactgcc ccacgccgat ggatttgggg tcgattttga
    12061 ccaactcggc aagcgggtct tgcagcctgc gggcgatgga cactgcgccg cgcagggaaa
    12121 catctaagtc gggaaactcg cgcgccgcca gttcggacgc agaataaatc gacgcgccgg
    12181 cttcggaaac gacgattttg tgcaggctgc tttcgggcat tccgcgcacc agttcgcccg
    12241 cgattttgtc ggtttcgcgg ctggcggtgc cgttgccgat ggcgatgagc ttcacaccgt
    12301 gttgtttaat caggcgcgac aacgttgcca acatattgtt ttcttgatgc aaatagacaa
    12361 tgaccgtatc cagcagtttg cccgtgtcgt ccaccacggc gcatttcacg ccgttgcggt
    12421 agccggggtc gagtcccaaa gtggtcagcc gcccggcggg cgcggcgagc agcaagtctt
    12481 tgagattgcg ggcgaacacg gtaatcgcgt cagtgtcggc agcttctttc aaacgattga
    12541 gagcttcgag ttccagcgac aaaaagattt tcgcgcgcca agtcaggcgc acggtgtcgc
    12601 gcagccattt gtggccgtct gaaaccttga agcggcgggc gatgatttgc tcgtattcgc
    12661 tttgctgcgt aatcggcgtg tcgtcgggct ggtatttgag cgcgatgttc aacacgcctt
    12721 cgttgcgacc gcgcaaaacc gccagcgcgc ggtggctggg catggcgcgc acgggttcgc
    12781 ggcggtcgaa ataatcgctg aatttttcgc cttcggtttc tttgccttca acgacttgcg
    12841 tgtggatttc ggcttcgttc cacagcttgt cgcgcagcgt gccgatgagt tccgcgtctt
    12901 cggcaaactg ctccatcaga atcgcgcgcg cgccatccag cgcggctttg gcgtcgggga
    12961 tgttttcgtt caggtagcct tgcgcggcgg cttccacgtc ctgcggctgt tcggcaagca
    13021 acacgtccgc cagcggctgc aaaccgtgtt cgcgagcgat ttgcgctttg gtgcggcgtt
    13081 tgggcttgta gggcagatac aggtcttcca gcgcggtttt gttgtcggcg gcttcgattt
    13141 gcgccctgag gtcgtctgaa agcttgcctt gctcttcaat gctttttaac acaacggcct
    13201 tgcgctcttc caactcgcgc aggtattgca gccgctcggc aagctggcgc agctgcgtat
    13261 cgtccagccc gcccgtggct tctttacggt aacgcgcgat aaaggggacg gtcgcgccgt
    13321 cgtccaaaag ctcgatagcg gcattgattt gcacggtagt ggcggagagt tcttgggaga
    13381 ggattcgggt aatgttcata aattgcctcc gtgatgccgt ctgaacagcc gacggcgcaa
    13441 tgtgggtaat cgtttggaaa agatgaagat aatactgata tactttgccc gatacaatct
    13501 gaaaggaatg tttatgaccg tcctgattac cggcggcacc ggctttatcg gttcgcacac
    13561 cgccgtctcg ctcgtccaat ccggttacga tgccgtgatt ttggataatc tgtgcaactc
    13621 gtctgccgcc gtcctcccgc gccttcggca aattaccggc agaaacatac cgttttatca
    13681 gggcgacatc cgcgactgtc agattttgag gcagattttt tcagaacatg aaatcgaatc
    13741 cgtcatccat tttgccggtt tgaaggcagt gggggaaagc gttgccgagc cgacaaaata
    13801 ttacggcaac aatgtttacg gcagcctggt gctggcggaa gaaatggcgc gcgcgggcgt
    13861 gttgaaaatc gtattcagct cgtcggcaac cgtttacggc gatgcggaaa aagtccccta
    13921 tacggaagat atgcgcccgg gcgataccgc taatccttac ggtgcgtcca aagcgatggt
    13981 ggagcggatg ttaaccgaca tccaaaaagc cgatccgcgt tggagcgtga ttttgttgcg
    14041 ctatttcaac ccgatcggcg cgcacgaaag cggacttatc ggcgaacagc ccaacggcgt
    14101 tcccaacaat cttttgccct atatctgtca agtggcttcg ggcaggctgc cgcaactgtc
    14161 ggtattcggc ggcgactatc cgacccccga cggtacggga atgcgcgact acatccatgt
    14221 gatggatttg gcagaagggc atatcgcggc aatgaaggcg aaaggcggcg ttgccggcgt
    14281 acatttgttc aacttgggtt cgggacgcgc ctattccgtt ttggaaatca tccgcgcctt
    14341 tgaggccgca tccggtttgc acattcctta ccgaatccaa ccccgccgcg ccggcgactt
    14401 ggcgtgttcc tatgccgacc cgtcccatac caaacaacaa accggctggg aaaccaaacg
    14461 cggcttgcag caaatgatgg aagattcgtg gcgttgggtc agccgcaacc ccggcagata
    14521 tggggattaa atgcaaaccg caggcaaaaa aaacatcctc gttaccggcg gcgcgggttt
    14581 tatcggctcg gcagtcgtcc gccatattat ccaaaacacc cgagattccg tcgtcaacct
    14641 cgacaagtta acctacgcgg gcaatctcga atcgctgacc gacattgccg ataatccccg
    14701 ctacgctttt gagcaagtgg atatttgcga ccgcgccgaa ctcgaccgcg tgttcgcgca
    14761 ataccggccc gatgccgtga tgcacttggc ggcggaaagc cacgtcgacc gcgccatcgg
    14821 ttcggcaggc gaatttatcc gaaccaatat cgtcggcaca ttcgacctgc tggaagctgc
    14881 gcgcgcctat tggcagcaaa tgccgtctga aaaacgcgaa gccttccgtt tccaccatat
    14941 ttctaccgat gaagtctatg gcgatttgca cggcacagac gatttgttta cagaaaccac
    15001 gccatacgcg ccgtccagcc cctactccgc ctccaaagcc gccgccgacc accttgtccg
    15061 cgcgtggcag cggacttaca ggctgccctc cattgtcagc aactgttcca ataattacgg
    15121 cccccgacaa ttccccgaaa aactcattcc tttgatgatt ctgaacgcac tttccggcaa
    15181 accactgcct gtgtacggcg acggggcgca aatccgcgac tggctgtttg tcgaagacca
    15241 cgcgcgcgcg ctgtatcagg tcgttaccga aggtgttgtc ggcgaaacct acaatatcgg
    15301 cggacacaat gaaaaaacca atctcgaagt gatcaaaacc atctgcgccc tcttggaaga
    15361 actcgccccc gaaaaaccgg ccggcgtggc acggtatgaa gatttaatta ctttcgtaca
    15421 agaccgcccc ggccatgatg cccgctacgc cgtcgatgcg gcgaaaatca ggcgggattt
    15481 gggctggctg cctttggaaa cttttgaatc cggcctccgc aaaaccgtgc aatggtattt
    15541 ggacaacaaa acccggcggc aaaacgcata aaacggcaac tgccgttcgg aacgcttagg
    15601 tacgggcaaa tagttttcag acggcatccc gacgcaatgc cgtctgaaaa cccatcgcaa
    15661 aggaagaagg aaaagatgaa aggcatcata ctggcaggcg gcagcggtac gcgcctatat
    15721 ccgattacgc gcggcgtatc caaacaactg ctgcccgtgt acgacaaacc gatgatttat
    15781 taccccttgt cggttttgat gctggcggga atccgcgata ttttggtaat taccgcaccc
    15841 gaagacaacg ctgccttcca acggctactc ggcgacggct ccgacttcgg catccgcctg
    15901 caatatgccg ttcaacccag ccccgacggc ttggcgcagg catttatcat cggcgaagaa
    15961 tttatcggca acggcaatgt ctgcctgatt ttgggcgaca atattttcta cggtcaatca
    16021 tttacgcaaa cattgaaaca ggccgccgcc aagacccacg gcgcaaccgt attcggttat
    16081 cgggttaaag accccgagcg tttcggcgtg gttgaatttg acgaaaactt caacgcctta
    16141 tccatcgaag agaaaccgca acagcccaaa tccgattggg cggtaaccgg cttgtacttc
    16201 cacgacaacc gcgccgtcga gttcgccaaa cagctcaaac cgtccgcacg cggcgaattg
    16261 gaaatttccg acctcaaccg gatgtatctg gaagacggct cgctgtccgt tcaaatattg
    16321 ggacgcggtt tcgcgtggtt ggacaccggc acgcaagaaa gcctgcacga ggcggcttcc
    16381 ttcgtccaaa ccgtgcaaaa tatccaaaac ctgcacatcg cctgcctcga agaaatcgcc
    16441 tggcgtaacg gctggctgac gaaaaaagat gtggaaacac gggcaaagcc tttggaaaaa
    16501 accgcctacg ggcaatacct gctgcgcctg atcggcaaat aaataccgtc tgaacgcccc
    16561 gacggctttc agacggcatc caaaccgtgc cgcgccggca ccttaaccga cctgccgcac
    16621 ggcgcgcata aaaaatcaaa ccgcaaaaaa acagcggtaa cgataccgcc aaaggacttt
    16681 atggacatca tcgacaccgc cctccccgac gtaaaacttt taaaaccgca agtcttcaca
    16741 gacgggcgcg gtttttttat ggaaacattc cgcgacggat ggttcaaaga aaatattgcc
    16801 gatcgaactt tcgtgcagga aaaccactcc aactccagca aaggcgtatt gcgcggcctg
    16861 cactaccaaa ccgaaaacac ccaaggcaaa ctcgtacgcg tagttgtcgg cgaagtgttc
    16921 gacgtggccg tcgatatgcg cgaaggttcg ccgactttcg gcaaatgggc gggcgcaacc
    16981 ttgtcggcgc aaaaccgata ccagctttgg atacccgaag gcttcgcaca cggtttttgc
    17041 gttttgggcg acgcggcgga agtcgtgtac aaatgcacgg actattacaa ccctgaaacc
    17101 gaacaggttt taatatggaa cgacccggca atcggcatag gctggccgct tcaaaccgcg
    17161 ccgctgcttt cgcccaaaga ccttgccggc aaaacgtggg cacaagccga aaagctccgc
    17221 cttacgcttt cccgataaaa aatgccgtcc gaacgtttca gacggcattt tctccgacag
    17281 gctacctacc cgccttcagt acgcgctgtg caaagaaaaa catcccggta acgaagaacg
    17341 ccaagcccaa ccaagaagcg gcggcatccc agttttgcag attcaacgcg agcggcgcat
    17401 ccgagccgcc gttggtcaca gagaaggcaa taataaacgc cataattaca ccaatcaggc
    17461 tttcaccgac aatcaggcct gccgcgaaaa gcgtaccgat acggtcggcg tttttcagac
    17521 ggccttcgcg gttttccgcc ttcttgccaa tgatgtgttt caacaccgcc gccaacaccg
    17581 cgcctgccac gatgggcata ttgacggacg gcggcagata aatgcccata ccgaccgcaa
    17641 ggacgggcag ggcaagttta ccgcctgatg attttttcaa caccaaatcg acgacgatta
    17701 ataccgctcc aatcgcgata ccggtaaaga tgtacgccca ttcgaggttg tgggcgaaaa
    17761 tgcccgacgc aatggtcgtc atcaaagtcg cttgaggggc tgccaaagcc tgcgccgcat
    17821 ccatgccttc gcgcggcatt gcgccggtaa agccgtaggc ttcgtaaagc agttccaaca
    17881 cgggcgaaat aaccagcgca ccaacgatac agccgataat cagggcgact tgctgccgcc
    17941 aaggcgtagc tttgagcagg tagccggttt tcaagtcttg caggttgtcg ttggaaatcg
    18001 aagccacgca gattactgcc gatccgcaaa aaagcgtcaa cgccagtaag aatttgcggt
    18061 tggcctcatc cgccaacaaa cctccggatt cgcctaccag cagcaaaacc agtgaaataa
    18121 cgacgatgga cacgatgccc acgccggaaa tcgggctgga agacgagccg accaaacctg
    18181 ccatataacc gcaggcggcg gcgaccaaaa agccgatgac ggaagccaaa agcgtgcaaa
    18241 ccgccaccaa aagccacgcc atgccgcccg taatgtgcga atcgccgata aagtggtaaa
    18301 acgacacgcc tagaataaac atcatagaca acacccagaa aatcatggct ttaggcgaca
    18361 aatcctgttc ggcgcgttct gtagcgggcg caccgccgcc aaaactcttg aacgacatct
    18421 tcatgccttc caccattggc ttgagcagca tcaaaagcgt ccaaaccgcc gcgataccta
    18481 tggttcccgc accgataaaa cgcacttttt ccttccacag cttcatcgca aacgccgcca
    18541 tttccatatc ggaaggttgc ggaatgtgtg aggagaaata cggcacggca ataccccaag
    18601 caatcgaaat gcccagcagg atggcgatgc cgcccgtcag tccgaccaaa taacccgcgc
    18661 ccaacaagac cagtgaaaag cccatcggca gttggaaaat tgccgtaccg cttttaaacc
    18721 aataactcgc gctatcggca atcacacgca gacctccggc gcaaaagctc atcaatcccg
    18781 ccaacgcgcc gccggccgcc agctctttga taccgctgcc gccctgacgg tcatcccctt
    18841 cttcatgacc gcccactttc aaaatttcag cagccgccac accttccgga taaggcaaat
    18901 cgcttttcac caccatcgcg taacgcagcg gaatggtgaa aatcaccccc aaaatcccgc
    18961 cggcaataca taaaagcgtc gtctgccaaa acgggaaacc gctccagtag cccgccatca
    19021 gcaaaccggg caggacgaag atgatggttg aaagcgtacc cgcagccgag gcttgggtct
    19081 gcaccatgtt gttttccaaa atattgctgc ctttgaaaaa ttttaaaacc gccatcgaaa
    19141 tcaccgccgc cggaatcgac gaggcaaagg tcagcccgac tttcaaaccg aggtaaacgt
    19201 tagacgcagt aaaaattaca gtgatcaatg cgccgagtat catgcctcgg agcgtcagtt
    19261 cgcggtattt ttctaccgaa ccggataaag atttattcat tattcttcct ttgacaacag
    19321 acgttcacat attgttggca tcacgccatg atgtcaagtt ttaaaaagaa cagttaaaaa
    19381 caattatccc accctgccca tacccaattg aaaataaaaa ctatttccaa acgaacaaaa
    19441 acagccgtat cagggaggtt ccccgatacg gctgcttgtt tccgaacctt aaaatcaatc
    19501 aaacaaatcg cgcagcttgt ctaaaaacga tttcttgcgc ggcgtttggt tttccaagcc
    19561 cgtagaaatc cgctcaaatt cttccaaaag ctctttttga cggtcggtca aattgacagg
    19621 cgtttcgaca acaatatggc agtacaaatc gccggtcgcg ctgctgcgta aagatttgac
    19681 acccttacct ttcacgcgca tcctcctgcc ggtttgggtt tctttgggga cggtgagctt
    19741 gactttgccg tccaaggtcg gcacttctaa ctccccgccc aaagcagccg tggcaaaact
    19801 aatcggcagt tcgcaatgca aatccaaacc atcgcgttgg aaaatcttat gcgcccgaat
    19861 gcggacggtt acatacaggt cgccggcagg cgcgccgtgc atacccggcc cgccttcacc
    19921 gctcaaacgg atacgctgcc cgtcatcgat accggcggga atattgactt ccaccgtctt
    19981 gaccgcctta ttccgcccca cgccacggca tttgacgcaa ggttctttaa tatgtttgcc
    20041 cgcaccgcgg caggtcggac aagtctgctg catacggaaa atcgcctgct ggatgtgcac
    20101 cgtacccgaa cctttgcaag tcgggcaggt ttccggggat gcccccggtt tcgcgccgct
    20161 gccgttgcag acatcacacg cttcataagt cggaatattg atgcgtttct tcacaccttt
    20221 tgcggcttct tcaagcgtga tttcgatacc gacctgcacg tcctcaccct gataatcagg
    20281 ctgggcgcgc cccgaaccac ctccaaacat ttggctgaaa atatccccaa agtcaaaacc
    20341 ctgcgcaccg ccaaatccgc caaaccctcc gaagcccccc tgtccgccgc cttcaaacgc
    20401 cgcatgacca tactggtcgt acatggcacg cttttccttg tcggacaaag tttcatacgc
    20461 cttttgtact tctttaaact tctcttccgc ctctttattg tcaggattgc ggtcgggatg
    20521 gtatttcatc gccaatttac ggtaggcttt tttaatctca tcatcggtag ctgctcttgc
    20581 cacacccagc gtcgcataaa aatcttgatt actcattttt tcatctaaaa ataaaatcac
    20641 ggcttaaaat gagggcaatt gcgcaaaaca caagacaaac agac
//
LOCUS       DAXDZG010000034        20590 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXDZG010000034 DAXDZG010000000
VERSION     DAXDZG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20590
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.034"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..221)
                     /locus_tag="ACFB78_001092"
     CDS             complement(<1..221)
                     /locus_tag="ACFB78_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269092.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEI"
     gene            386..1309
                     /locus_tag="ACFB78_001093"
     CDS             386..1309
                     /locus_tag="ACFB78_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC1269093.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLHPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLARGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDAFYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1388..2029)
                     /locus_tag="ACFB78_001094"
     CDS             complement(1388..2029)
                     /locus_tag="ACFB78_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC1269094.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2084..2875)
                     /locus_tag="ACFB78_001095"
     CDS             complement(2084..2875)
                     /locus_tag="ACFB78_001095"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC1269095.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            3041..3116
                     /locus_tag="ACFB78_001096"
     tRNA            3041..3116
                     /locus_tag="ACFB78_001096"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Thr,seq:tgt)
     gene            3258..4049
                     /gene="panB"
                     /locus_tag="ACFB78_001097"
     CDS             3258..4049
                     /gene="panB"
                     /locus_tag="ACFB78_001097"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC1269096.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKVHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4172..5008
                     /gene="panC"
                     /locus_tag="ACFB78_001098"
     CDS             4172..5008
                     /gene="panC"
                     /locus_tag="ACFB78_001098"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC1269097.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5335..7173
                     /locus_tag="ACFB78_001099"
     CDS             5335..7173
                     /locus_tag="ACFB78_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC1269098.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7313..7894
                     /locus_tag="ACFB78_001100"
     CDS             7313..7894
                     /locus_tag="ACFB78_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC1269099.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB78_001101"
     CDS             7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB78_001101"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC1269100.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8757..8832
                     /locus_tag="ACFB78_001102"
     tRNA            8757..8832
                     /locus_tag="ACFB78_001102"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8790..8792,aa:Gln,seq:ttg)
     gene            8845..8920
                     /locus_tag="ACFB78_001103"
     tRNA            8845..8920
                     /locus_tag="ACFB78_001103"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8878..8880,aa:Gln,seq:ttg)
     gene            9088..10071
                     /locus_tag="ACFB78_001104"
     CDS             9088..10071
                     /locus_tag="ACFB78_001104"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC1269101.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKGRGAEGVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10138..10710
                     /locus_tag="ACFB78_001105"
     CDS             10138..10710
                     /locus_tag="ACFB78_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC1269102.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10836..12005)
                     /locus_tag="ACFB78_001106"
     CDS             complement(10836..12005)
                     /locus_tag="ACFB78_001106"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC1269103.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB78_001107"
     CDS             12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB78_001107"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC1269104.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13736..14812)
                     /locus_tag="ACFB78_001108"
     CDS             complement(13736..14812)
                     /locus_tag="ACFB78_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC1269105.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(14809..15669)
                     /gene="cysW"
                     /locus_tag="ACFB78_001109"
     CDS             complement(14809..15669)
                     /gene="cysW"
                     /locus_tag="ACFB78_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC1269106.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAVLGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIVVPVNAVLGVAMAWLLIRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(15858..16694)
                     /gene="cysT"
                     /locus_tag="ACFB78_001110"
     CDS             complement(15858..16694)
                     /gene="cysT"
                     /locus_tag="ACFB78_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC1269107.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     DGFRNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGSIPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVLQWALGRRSGA
                     KG"
     gene            16875..17207
                     /locus_tag="ACFB78_001111"
     CDS             16875..17207
                     /locus_tag="ACFB78_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269108.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            17538..18047
                     /locus_tag="ACFB78_001112"
     CDS             17538..18047
                     /locus_tag="ACFB78_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC1269109.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(18279..18860)
                     /locus_tag="ACFB78_001113"
     CDS             complement(18279..18860)
                     /locus_tag="ACFB78_001113"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC1269110.1"
                     /translation="MEHKLPQLPYGPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLALVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAA"
     gene            19023..20429
                     /gene="dnaB"
                     /locus_tag="ACFB78_001114"
     CDS             19023..20429
                     /gene="dnaB"
                     /locus_tag="ACFB78_001114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC1269111.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     IIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYVPEEAKIED"
BASE COUNT         4824 a         4884 c         5864 g         5018 t
ORIGIN      
        1 tgaatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
       61 gcaagtgcgg cgcggctgtt ttcgctattc aggtaggtaa gggaagtttc cagtttccat
      121 tttcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agcggataaa
      181 ggcaggatgg cgggcaaggc gggcaaaaag atgcgtttca tattttctct tattgatatt
      241 aattcttatt taagaatagt aatgaatatc cgataattct gtctaaataa atttttaaat
      301 caaaaaaaac gatatatttg tttttatcaa ttatgttttg atatgccgtc tgaaaagttt
      361 gtgaaaaacg gttacaatcc gccgtatgaa aaaacgcaat aatcctcttc cgctgttgaa
      421 cggtgtcaaa cccagttatt tggtgctgcc gcatgaaaag cagttttacg ggctgccgct
      481 gctgcatttt ctgtgcatcc gctttccttt tgtgggtgcg gacgattggc ggaggcggtt
      541 gaacagcggt tttgtggtcg gttcggatgg tgcggcgttg gatgaacatt ctttgttcga
      601 gccgggtaag acgatgtttt attatcggga aaccagcagt gaggatgagc cgcgtattcc
      661 gtttgaagaa aaaattttgc atattgatga gcatttgatt gtggtggaca agccgcattt
      721 tctgcccgtc atccccggcg ggcgtttttt gcgggaaacg ctgctcacac gtctgcgcct
      781 gcaccctgaa ttgcagcatt tgaatgttga agacattacg ccgattcacc gcttggacaa
      841 ggatacggcg ggtgtgatgc tgctgtcgca caatcctgcc acgcgcgggg cttatcagac
      901 gatgtttcaa aacaaaacgg tatggaaaac gtatgaggcg cttgcgccga caaggacgga
      961 tttgccgtat ccgctcgatg tggtttcgcg tttggcgagg ggggagaaat tttttacgac
     1021 gcaagaggcc gaaggcgaac caaacgcaca tacgacggtc gaactgattg aaaacagggg
     1081 ggaattcagc ctttaccgcc tcacgccgca taccggcaag aaacaccagt tacgcgtgca
     1141 tatgatgggt ttgggtatgc cgctgttgaa tgacgcgttc tatcccgtgc cgtctgaagc
     1201 gggtagcgag gattatcgga aacctttgaa gcttttggca aaaaagattg cgtttgcaga
     1261 tcctttgtcg ggtcgggaaa gggtgttttg cagcgggttt tgcctgtaat gagagggaac
     1321 gcaaacccgt atcggcagat gccgccggat acgggtttga tattatcgat gctgtctgaa
     1381 aggggcttta tccgttcagg catacgggtg cgaagcggag gatggtgtcg cgcaggacga
     1441 tgagtttgcc gtgaaagaag tgcgtcgaac cggcgatggt aatgacgggc aaatcttgcg
     1501 gttctgccca tttcagtgct ttttcgattt cgacgacctc atcttccgcg ccgtgtatca
     1561 tcagcgtttt ggcaacgttg ggaacggcgg acggctcggg gcggtcggta tagtggcaga
     1621 ctgccgcacc gatgagcagc aataaatcgg gtatgcgcac ttgtgcggca aatgtggcga
     1681 catagccgcc gaaggaaaag ccggataagg caaattccgg ggcttcggga tgttgggcgc
     1741 gggcatagtc gatgacggcg aggcagtctt gcgtttcgcc gcgtccgtaa tcatgtgtgc
     1801 cttcgctgcc gcctacgccg cgaaggttgg gaaggtagca gtggaagccg agcttgctca
     1861 atgctttggc ggcggtttgg atgactttgt tggtgttcgt cccgccttgg agggggttgg
     1921 ggtggttgat gacggcaaca ccgcgtgccg gtacttgttc ggacgggata tggatggttt
     1981 ctaaaatgcc ggcagggccg ggtatgtgta tggtttcggg tttcagcata atggtttcct
     2041 cttgattttc cgcatattcc gatgcagccg cgaagatggc ggcttaaagg tggaaatgct
     2101 tgccgttgtt ggggttggac gctttcaggc cggcgagcat gcggtggaag tccaatccgt
     2161 atgcgttgct cagtttgtcg gcacgttttt tgagtttgtc tatgttttgc tctttggggc
     2221 tgctttggaa ggccattacc gcgacattgc cgtggctttc ggcaggaagt tccaatacgc
     2281 gcccttcaaa aacgctcaac aaccgttcga tgaagcgttg atagcgtttg tcgccgctcc
     2341 accagttggt aacgaatatg ccgtctgaag agagtgcgtt gcggcagtct ctgaaaaacg
     2401 gttcttcaac cagcgtatcg atgatttgtt cgccgtcgaa tccgtccacc aagatgatat
     2461 cggtgttgtg gcggaagact ttgatgtatt ccgcaccgtc tgcttcgata atttcaaatt
     2521 ttccgccctc gaaaggcaac tcaaacaggt tgcgggcaat ggcgatgacc tgaggattga
     2581 tgtccacggc ggtttggcgc gtgtcgggca ggtaggtgtc tgtccagcgt gcaaacgaac
     2641 cgccgcccaa gccgatttgg gtgatatgtt gcggcaggcg ttccgcaaac agcagccagc
     2701 ccatcatcgc gcggctgtaa gagagcacca gctcggacgg gcggtcgagg ttcatcgagc
     2761 tttgaacggt gtcgctgccc aagtgaagcg aacggatatt gccttcttcg gaaataccga
     2821 cttcgggaaa gccggatttt gcaggacgca ggcggcggta gggatgtctt gccatcggtg
     2881 tgaacggtcg gtttgaaagg cggatatttt atcagaatgc cgatgcttgt tctgtttcaa
     2941 attaattttg ttttaaataa gatacttagt gtatttttat gagggggtcg aacaaatttc
     3001 ttgccaaagt gtgaagttgc ctgcataatt cgccttcttt gccggtatag ctcagttggt
     3061 agagcacctg acttgtaatc agggggtccc gagttcgact cttggtgccg gcaccagatt
     3121 tgacagcccg attgtgtaaa acagtcgggc tgttttgcat ttgcggcacg gtcggtggca
     3181 gggctgcggg catttcacta taatagcccg tttcaaactg aaaaggccgt ctgaaaaggg
     3241 cggggtaaca atatctgatg attactgtga acacactgca aaaaatgaag gcggccggcg
     3301 agaaaatcgt tatgctgacc gcttacgaat ccagttttgc cgcgctgatg gacgatgccg
     3361 gcgtggatgt gctgttggtc ggggattctt tgggaatggc ggttcagggg cgacagtcga
     3421 cgctgccggt cagcctgcgc gatatgtgct atcacaccga atgtgtagca cgcggtgcaa
     3481 aaaatgcgat gattgtcagc gatttgccgt ttggtgcata tcagcagagt aaggagcagg
     3541 cgtttgccgc cgccgccgaa ctgatggctg ccggcgcgca tatggtcaaa ctcgaaggcg
     3601 gcgtgtggat ggcggaaacg actgaatttc tgcaaatgcg cggaattccg gtctgtgccc
     3661 acatcggtct gaccccgcaa tccgtgtttg ccttcggcgg atataaagtt caggggcgcg
     3721 gcggcaaggc gcaggcgttg cttaacgatg ccaaggtgca tgatgaagcc ggagctgccg
     3781 tcgtactgat ggaatgcgtg ccggcggaac tggcaaaaaa ggtaactgaa actgtttcct
     3841 gtccgaccat cggcatcggg gcgggcgcgg attgcgacgg tcaggttttg gtgatgcacg
     3901 atatgctcgg tattttcccg ggcaaaacgg cgaaattcgt caaaaacttt atgcgcgggc
     3961 aaagcagcat ccaggctgcg gttcgggcgt atgttgccga agtcaaagcc aaaaccttcc
     4021 ctgctgcgga acatattttt gcagattgaa caaggtctgc tgccgatgcc gtctgaaagc
     4081 cgtttcagac ggcattttgt tttgccgtgc gcggcgcgta taatcggcgc gttttgtcgg
     4141 gcaggaagcc cgaaggataa ggattaccgt aatgcaaatc atacatacca ttcgagaact
     4201 gcgcacgtgg cgtgaaaata taggaaaggt ggcatttgtg ccgaccatgg gcaatctgca
     4261 tgaagggcat cttgcccttg ttcgcgaagc cagaaaacgc gccgacaacg ttgttgtcag
     4321 catattcgtc aaccgcttgc aattcggtca gggcgaggat ttcgacaaat atccgcgtac
     4381 tttgcaacag gatgcggaca aacttgccgc cgaaggcgtt gccgttgttt tcgcgcccga
     4441 tgagaaagaa ctctatccga acgtggaaca gcgttacaac gtcgaaccgc cccatttgca
     4501 aaacgaattg tgcggcaaat tccgccccgg ccattttcgc ggcgtggcga cggtggtgtc
     4561 caagctgttc aatatcgttt tgccggatgt tgcctgtttc ggcaaaaaag attaccagca
     4621 gcttgccgtg attaaaggtt tgaccgagga tttgaatttc gatatcgaaa ttgtccccgt
     4681 cgatacgggg cgggcggcgg acggtttggc actctccagc cgcaaccggt acttgagcgt
     4741 tggggaacgc gccgaagcac cgcgcctgta ccgcgagctg caggctgttg ccgaatcgct
     4801 gaagcagggc ggtttggatt atgcgggttt ggagcggcag gctgccgacc atttgaccgc
     4861 tgcgggctgg ctggtcgatt atgtggaaat ccgccgcgcc gatacgctcg aaatggcgcg
     4921 ggcgggagat aagaaactgg tggtcttggc cgccgcccgt ctggggacga cgcgtctgat
     4981 tgacaatgtg gaagtcggcc tgccttagcc cgccggagcc gggaatgccg cctgaagcgg
     5041 atttgcgttt cagacggcat tttgccgtcg gctgtgcttc ggaataatgt cggaaaatag
     5101 acggaatgaa ataaatttct ttttgttcaa caaattaaac tgtcaaacca aataataagg
     5161 caaggattgt ccggcatcgc atacaggcaa accgacgcaa tctcaaaaca cagccctgcc
     5221 ttcgggcggg ttttgcaaat ctacaaaccg tttggcttgt gataaagtga cgccttgtct
     5281 agatactcaa tatgccgtct gaaggttcgg acggatgtcg gataaaggat gattatgtta
     5341 cccgcccgtt tcactatttt atctgtcctc gcagcagccc tgcttgccgg acaggcgtat
     5401 gctgccggcg cggcggatgt ggagctgccg aaggaagtcg gaaaggtttt aaggaaacat
     5461 cggcgttaca gcgaggaaga aatcaaaaac gaacgcgcac ggcttgcggc agtgggcgaa
     5521 cgggtcaaca gggtgtttac gctgttgggc ggtgaaacgg ctttgcagaa agggcaggcg
     5581 ggaacggctc tggcaaccta tatgctgatg ttggaacgca caaaatcccc cgaagtcgcc
     5641 gaacgcgcct tggaaatggc cgtgtcgctg aacgcgtttg aacaggcgga aatgatttat
     5701 cagaaatggc ggcagatcga gcctataccg ggtgaggcgc aaaaacgggc ggggtggctg
     5761 cggaacgtat tgagggaagg gggaaatcag catctggacg ggttggaaga ggtgctggcg
     5821 caatcggacg atgtgcaaaa acgcaggata tttttgctgc tggtgcaagc cgccgtgcag
     5881 cagggtgggg tggctcaaaa agcatcgaaa gcggttcgcc gtgcggcgtt gaagtatgaa
     5941 catctgcccg aagcggcggt tgccgatgcg gtgttcggcg tacagggacg cgaaaaggaa
     6001 aaggcaatcg aagctttgca gcgtttggcg aagctcgata cggaaatatt gccccccact
     6061 ttaatgacgt tgcgtctgac tgcacgcaaa tatcccgaaa tactcgacgg ctttttcgag
     6121 cagacagaca cccaaaacct ttcggccgtc tggcaggaaa tggaaattat gaatctggtt
     6181 tccctgcgta agccggatga tgcctatgcg cgtttgaacg tgctgttgga acacaacccg
     6241 aatgcaaacc tgtatattca ggcggcgata ttggcggcaa accgaaaaga aggtgcgtcc
     6301 gttatcgacg gctacgccga aaaggcatac ggcaggggga cgggggaaca gcggggcagg
     6361 gcggcaatga cggcggcgat gatatatgcc gaccgcaggg attacgccaa agtcaggcag
     6421 tggttgaaaa aagtgtccgc gccggaatac ctgttcgaca aaggcgtgct ggcggctgcg
     6481 gcggctgccg aattggacgg aggccgggcg gctttgcggc agatcggcag ggtgcggaaa
     6541 cttcccgaac agcaggggcg gtattttacg gcagacaatt tgtccaaaat acagatgctc
     6601 gccctgtcga agctgcccga caaacgggaa gccctgatcg ggctgaacaa catcatcgcc
     6661 aaactttcgg cggcgggaag cacggaacct ttggcggaag cattggcaca gcgttccatt
     6721 atttacgaac agttcggcaa acggggaaaa atgattgccg accttgaaac cgcgctcaaa
     6781 cttacgcccg ataatgcaca aattatgaat aatctgggct acagcctgct ttccgattcc
     6841 aaacgtttgg acgagggttt cgccctgctt cagacggcat accaaatcaa cccggacgat
     6901 accgccgtta acgacagcat aggctgggcg tattacctga aaggcgacgc ggaaagcgcg
     6961 ctgccgtatc tgcggtattc gtttgaaaac gaccccgagc ccgaagttgc cgcccatttg
     7021 ggcgaagtgt tgtgggcatt gggcgaacgc gatcaggcgg ttgacgtatg gacgcaggcg
     7081 gcacacctta cgggagacaa gaaaatatgg cgggagacgc tcaaacgcta cggaatcgcc
     7141 ttgcccgagc cttcccgaaa accccggaaa taatgcaggc ccatcctttc agacggcata
     7201 aggtttgccg ggaagccggg gcattcgggc aaacggcacg cagttcgcac gcgttttgca
     7261 cggcacgccg cacccgtcgg ccggcaggat gacacccgtt aaggaaactc tgatgaaaca
     7321 caccgtatcc gcatcggtca tcctgctttt gaccgcttgc gcgcaattac ctcaaaataa
     7381 cgaaaacctg tggcagccgt ccgaacacat cagcagtttt gcagcggaag ggcggttggc
     7441 agtcaaagcg gaagggaaag gttcgtatgc aaatttcgat tggacatacc aaccgcccgt
     7501 ggaaaccatc aatatcaaca cccctttggg cggcacgctc ggacagttgt gtcaagacag
     7561 ggacggcgca ttggcagtgg acggcaaagg aaatgtctat caggcagagg gtacggaaga
     7621 cttgagcagg cagctggtcg gtttcaaact gccaatccaa tatctgcata tctgggcgga
     7681 aggcaggcgt gtggcgggcg cgccttaccg catccgttca gacggcatat tggaacaata
     7741 cggttggaca atcggcagaa ccgccgacag tggggggcaa gtccgaacgt tgcaactgaa
     7801 taacggaaat ttgaacatca ggctggtttt caccgaaatt ggaatgccgt ctgaaaccga
     7861 aaccccggaa cgctgtgcgg cgcgcacgag ataaggcgga cagatgaata ttgcggacgg
     7921 acggcaggcg tttcccgcac ctgcaaaact gaatctcgat ttgaggatta ccggcaggcg
     7981 ggaagacggt tatcacaata tcgaaagcat attctgcctg atagatttgc aggataccgt
     8041 atatttgaaa ccgagggacg acggcaaaat catcctgcac aatcctgtcg gcggaatacc
     8101 gcaggaagcc gatttgagct accgtgccgc atcgttgctg caaaaatatg cgcgcaacct
     8161 tgccggcgtg gaaatatggc tggacaaaaa aatcccgacc ggcgccggct tgggaggggg
     8221 gagttccgat gccgcaacgg ttttgctggt gttgaaccgt tggtggcagt gtggtttgac
     8281 gcagtggcag ctcattgatt tgggtgcggc tttgggtgcg gacgtaccgt tttttatttt
     8341 cggaaaaaat gcttttgcaa gcgggatagg tgaaaaactg ataggaatgg atattccgaa
     8401 acagtggtat gttattgtca aacctcccgt ccacgtttcc accgcaaaaa ttttcacata
     8461 cgaaggcttg acacgggatt ctgcctcaag cataatgccg actttccaaa acctgcaacc
     8521 gttcagaaac gatatgcagg cagtggtatt taaagaatac cctgaagttt ggaaagctta
     8581 ttccgagttg tcgaaatacg gttctgcaat gatgaccggc tcaggagcct gtatatttgc
     8641 agcatttcaa gctaggaata gcgcatacaa tatataccga caagtttcag gtttgtacga
     8701 ggcatatttg gcagagggtc tttcaaaaca tcctttgttg tccgtataaa cattgttggg
     8761 gagtcgtcaa gcggttaaga cactggattt tgattccagc atgcgaaggt tcgaatcctt
     8821 cctccccagc caagccaaac aagttgggga gtcgtcaagc ggttaagaca ctggattttg
     8881 attccagcat gcgaaggttc gaatccttcc tccccagcca gacaaaagcg tgtaagcctg
     8941 cttacacgct ttttatttca tagaaataaa aatattgaaa cgcctttgtt tgtgtcggat
     9001 gttgcaggta taatgccggg cttggtacaa gcggagggaa gcattgtgtt ttctgagcgg
     9061 aagttaaaca taaaatcagg tgagaatatg gctgcgtacg acagtttgat ggtatttaca
     9121 ggcaatgcca atcccgaatt ggcacaacgt gttgtcaggc atttggacat ttctttgggc
     9181 aatgcttccg tatccaagtt ttcagacggc gaagttgccg tcgaactgtt ggaaaacgta
     9241 cgcgggcgcg acgttttcat ccttcagccg acctgtgcgc cgaccaatga caacctgatg
     9301 gaaatcctga cgatggcgga tgcactgaag cgtgcttcgg caggtcgtat taccacagcc
     9361 attccgtatt tcggctatgc gcgccaagac cgccgtccgc gttccgtccg cgttccgatt
     9421 tctgccaaac tggtggcaaa tatgctgtat tcggcaggga tcgaccgtgt tttgactgtc
     9481 gatttgcatg ccgaccagat tcaaggtttc ttcgatattc cggtggacaa tatttatgcc
     9541 accccgattc tgttgaacga catcaagcaa cagcggattg aaaatctgac cgtcgtcagc
     9601 ccggacatcg gcggtgtcgt ccgcgcccgc gccgtggcaa aatccttgaa tgccgacttg
     9661 gcaatcatcg acaaacgccg cccgaaagcc aatgtggcgg aagttatgaa catcatcggc
     9721 gatattcaag gcagaacctg tctgattgtg gacgatatga tcgacactgc aaatacgctg
     9781 tgcaaagccg ccgtcgccct gaaagggcgg ggggcggaag gtgttctagc ctatgccagc
     9841 cacgctgtat tctccggaga ggcggtcagc cgtattgcct catccgaaat cgaccaggtg
     9901 gtcgtaaccg ataccattcc tttgtctgaa gcggctaaaa actgcgaccg tatccgtcag
     9961 gtaacgattg caggtttgtt ggccgaaacc gtccgccgca ttagcaatga agaatccgtc
    10021 tcatatcttt tcaatgaaga agtgatgaca ggcagcatgt tgctgccgta accccgatgc
    10081 cgtcttaagc tggtcgcggc cgatgacgac gattttacct aacttggagt atttaacatg
    10141 acttatgaaa ttcaagcctc tgttcgtgaa gcacaaggca ctggtgcgag ccgccgcctg
    10201 cgtcgcgaag gccaaatccc cggtattctg tacggtgaag gtcaagagcc cgttgcgatt
    10261 gccgtagatc acaaaaccgt attctacgca ctggaaaaag aatctttcca tactgcgttg
    10321 attaaattgt ctctgaacgg cgaaaccaaa gacgttatcg tccgcgattt ccaaatgcac
    10381 ccgttccgcc gcgaagtcca acacatcgac ttccaagctg tgaaagccga tcaacttgta
    10441 cgcatccgcg ttcccctgca cattgttaac gctgaaaatt ctcaagccgt taaattgcaa
    10501 ggcggtcgcg tatctctgct gaacacggct gttgaagtgg ttgcgttgcc tgccaatatc
    10561 cctgctttct tggatttgga ttgtgccgaa gtggttgccg gcgacatttt gcacttgtca
    10621 gacatcaaac tgcctgaagg tgtagaaagc gtttccctga aacgtaacga aaatctggct
    10681 gttgctaccg ttaccggtaa aaagcgctaa cttacttcca tcagcagggc ggcgcgtatg
    10741 caatacgtac cgccctgttg ttttatgccg tctgaaccgt gtttcagacg gcatttcttt
    10801 atttgttgga aaagcgggat atttgaaacg gcagattact gccctgtcag acgcgtccaa
    10861 agcctttgcc accggcttct tttttctaca ttttccagtg cgacgatttc tttttcggca
    10921 atggtatgtc cgttttgcct gattttgatt tttcctaaaa tctgcccttt ttttaccggg
    10981 gcgggaatcg gctgtatggt ttccaaaatc tgttctgcca ttttcgcttc tttatgtggc
    11041 agagtgatgt aggcttcttt gagaaagcct gcgcggacgg tttttttgct gcctccggaa
    11101 atttggattt gggcaacggt tttgcctttc ggatatattt tgggcgtatc gaaggcctgc
    11161 aatgcccggt tcagcagctt gctgttgtcc gatgcgcggg tttccgccga ttccgaacct
    11221 agtgtgatga caaggatgtg cctgccgttg ccggagtatg acacggcaag gttgtagccg
    11281 ccgctttctg tgtgcccggc tttcaggccg tttacattgt tgtccctata taaaaggata
    11341 ttgcggttgt tttgttctat gttttcaaac ttgaacgatt tgatggaaaa cagcgggtaa
    11401 tattccggaa agtcgcgcat caatgcttca gacagcaggg agaggtcttt ggcggtggaa
    11461 acctgtcctt ctctacccaa gcctgtcggg tttttgaata cggtgttctt catgcccaag
    11521 cgtcgggctt ctttgttcat ttgttgcaca aaattttcaa tcgagccgtt gcccagccgg
    11581 tcggcaaggg ttagggcggc atcgtttgcg catagggcaa tcatgccttt taagagtttg
    11641 tcggtgctga ccgtatcgcc gggacgtaca aacattctgc ttccttctga agcccatgcg
    11701 gattcgggta tttttaagtt ttcttcagat tggatatttc ccgatttcat gtttttgaaa
    11761 accaaatatg cggtcatcag ttgggttagt gccgccggtt cgacaggggt attggtgttt
    11821 ttggcggata aagtctgcct gctttgaagg tcgataacga tgtgtgccgc cgtgagggtt
    11881 tcgggtgttt ggagcgtggg ggcggcgtgt accgtcggtc tgttgggcgc gggcgatgca
    11941 gccgttgcgt gagaaacgcc taagatgatg ggaagaagga cgggcaggat tttatgtgct
    12001 gtcatgaaat attctaaatt gtgtgagtgt ttcagcctgc cgattatacg cttagggtgt
    12061 ctgatcgggc ggatttttct tgctttcgcg ccgtcttggg cgtatggttt tgggttttgc
    12121 gattttaata aaccgattat cccgcattga attatgaaca cgccccttcc ttattccgat
    12181 tacctcatcc gcatcctgac ggcatctgtc tatgatgtag cggtcgaaac gcctttggaa
    12241 ccggcacgca gcctttctgt acgtttgaaa aacaacatcc ttttgaaacg cgaagatttg
    12301 cagccggttt tttcgttcaa aatacgcggc gcgtacaaca aaatgtccaa gttgccgaaa
    12361 gatgcgctcg cttgcggcgt gattgcggca agcgcgggca atcacgcaca aggcgtggcg
    12421 ttgtccgcac agcgtttggg ctgccgtgcc gttatcgtca tgccggagac tacgccgaaa
    12481 attaaagtgg atgcggttaa aagccgtggc ggcgaggtgg ttttgcgggg tgtttcatac
    12541 aacgatgcct acgattatgc gatggagctg gcggaacagg aagggctgac ctatatcgcg
    12601 ccgtttgacg atcccgatgt gattgcggga caggggacgg tggggatgga aattgtcagc
    12661 cagcatcccg atccaatccg cgccgtattc gtaccgatag ggggcggcgg tttggcggcg
    12721 ggcgtggcgg catttatcaa acaggttcgt cccgaaatta aagttatcgg cgtacagacc
    12781 aacgattcct gctgtatgaa gcagtcggtc gaagcgggcg aaatcgtcca tttgaaagat
    12841 gtcgggctgt tttcagacgg tacggcggtc aaagtcgtcg gaaacggaac cttccgcctc
    12901 tgcaaagaac ttttggatga aatcattaca gtcgataccg atgcggtttg cggcgcggtc
    12961 aaggatattt tcgatgacac gcgcagcatt accgagccgg ccggggcgtt ggcgttggcg
    13021 ggtctgaaag cctatattgc ccgagaaggt gcggaaaacc aaaccctgat tgccgttacc
    13081 agcggtgcga atatgaattt tcaccgtttg cgccacgttt cggaacggag cgaattgggc
    13141 gagggcaacg aaggtatttt tgccgttacc atccccgaag aacgcggcag cttccttaag
    13201 tttgtcaata tattgggaaa taggaatatt accgagttca actaccgcta cggcgacgat
    13261 gaaaaagcgc atatctttgt cggacttcaa gcggcaggcc cgcaggattt ggcggttatc
    13321 ggcagccggt tggatgaggc gggattgccc aatgtcgatt tgaccaataa tgagattgcc
    13381 aaaatccata tccgctatat ggtcggaggg cggacggaca aagtagggca cgagcgtctg
    13441 gtcagttttg agtttccgga gcgtccgggc gcgttggcgc gctttttgaa ccatatgcag
    13501 ggcggttgga acattacgct cttccattac cgcaaccacg gtgcggatta cgggcggatt
    13561 ttggtcggta tcgacgtgcc gccgcacgat gccgccgcat ttgacggttt cttggaaagt
    13621 ctgggataca gctatcacga ggaaacgcaa aatgccgcgt acaagctgtt tcttgcctga
    13681 cgtttgacac gcaatgccgt ctgaaagcct ttcagacggc attgcgcttt cagggttaaa
    13741 tcgaatattc aatcaactcg ttttgagaaa aaacataaac ctgtttcgga attaagttta
    13801 attttttgcc ttcggcgatt gggtagcgcg cggcatcgct gccagccagt gtgatatgca
    13861 cgtcttgttt gccgtgtttc accaaaacgt gcgtcaatgc gccgacggcg tggatttttt
    13921 cgatttcggc acggatcatc ggggtttcgt gttcggcggc gatctgccat tcgtgcgggc
    13981 ggatatagcc ggtggcggtt tgctcctgcc atttgtattg cgcgtccaat ttccacgcga
    14041 agccgttgta atgccagatc cctttttcga tgcgtccttc aaatgcgtcg gcttcgccga
    14101 ggaactcggt aacgaaggcg ttttcgggtt tgcggtaaat ggcttcggca ctgccggttt
    14161 gttcgatttt gccatggttc atcacgacga tttcgtcgga aacttcgagg gcttcttctt
    14221 ggtcgtgagt caccagaatg ctggttacgc cgaggttgtg atgaatgtcg cgcagccagg
    14281 tgcgtaattc tttgcgcact ttggcatcca gcgcgccgaa gggttcgtcc aaaagcagga
    14341 gtttcggttc gaccgcaagc gcgcgggcga gggcgatgcg ctggcgttgc ccgccggaga
    14401 gttggtgcgg ataggatttg gcgaggtggg agagttgtac gagtttgagc aattcttcga
    14461 ctttggcgcg gatttgtcct ttggacgggc gttcgggctt gggcaatacg gtcaaaccga
    14521 aagcgacgtt gtcaaacaca ttcatgtggc ggaagagggc gtagtgttgg aacacgaaac
    14581 cgactttgcg ctcgcgcaca tgcttggcgg ttacgtcttg gccgtcaaag agaatttggc
    14641 cgccgtcggc gttttccagt ccggcgataa tgcgtaaaag tgtggttttg ccgcagccgg
    14701 agggaccgag cagggaaacg agtttgccgg cggggacgtt gaggttgatg tttttcagcg
    14761 cgtgaaaatt gccgaagcgt tttttgttta agttttgaat ggtaatactc atactgcgtt
    14821 cctttcggcg gcggcgagtt ttttgtcttg taatttggtg atgatgttct gcaccgccag
    14881 cgtcgccagt gctaaaagtg ccaatacgcc ggagagggag aatgcgccgg tgaagttgta
    14941 ttcgttgtag aagatttcga ccaaaagcgg gatagtgttg gtttcgccgc gtatgtgtcc
    15001 cgataccacg ctgaccgcgc cgaactcgcc catcgcacgg gcgttggtga ggatgatgcc
    15061 gtagagcagc gtccatttga tgttgggcag ggtaacgcgc caaaacatct gccaaccgct
    15121 tgcgccgagt accaatgccg cctgttcttc gctgtcgccc tgtgtctgca ttaaagggat
    15181 gatttcgcgt gcggcaaagg gaaaggtaac gaacagcgcc gtcaaaacaa tgccgggaat
    15241 ggcgaagata atctgtatgc cttgcgcttc cagccggctg cccaacgccg tgtgcgcgcc
    15301 gaacaataag acgaacatca aaccggcaac cacgggcgat acggaaaacg gcaaatcgag
    15361 cagggtggtc agcaactgct tgccgagaaa gtcaaaacgg atcaacagcc acgccatcgc
    15421 cacgcccaag acggcattga cgggaacgac aatcagtgcg gtaatcagcg tcaatttgac
    15481 ggcagaccac gcttcgggat cgcttaagga tttcaggtac aaatcccaac cgccttttaa
    15541 ggcttcgtaa aacacggcga cgagcggcac gaccagcatc agcagcagaa agcccagcac
    15601 ggcggcaatc agcaacatgc gcagccggcg cggttcggtc aggttgggat tggcggaata
    15661 gggtttcatg gcggtggtgt tctctggttt gttgtttaag gtgttcggat gccgtctgaa
    15721 agggattgag gggtttcagc tttcgtcggt cggattcttg aatccgacaa ggttgctgaa
    15781 tcgcaaatgt ttgtcggata caagtatccg acctacgaat tctgtgtttt ggggagcgga
    15841 ttcgttttca gacgacctca acccttcgca cccgaacgcc tgcccaacgc ccactgcagc
    15901 acgttcagcg caaacagaat cacaaacgaa accagcagca taaacaacgc caccgccgac
    15961 gcgccctgcg cgtcgaattg ttccagcttg cccgtaataa tcagcggcag gatttcggaa
    16021 atcatcggaa tgctgcccgc gataaaaatc accgaaccgt attcccccgt tgcccgcgcg
    16081 aacatcatgc ccgcgccggt caagagtgcc ggcgtgattt ccggcagcag cacgcggcgg
    16141 aacgttgtga aacggtttgc gcccaaagtc gccgccgctt cctcatattc gcccgacaat
    16201 tcctccaata ccggctgcac ggcgcggacg ataaagggca ggctgacgac aaccagcgca
    16261 atccaaatgc cgacgggcgt aaacgcgatt ttgatgccca aaggttcgaa aaaacggcct
    16321 atccaaccgt tgggcgcata cagggccgcc aacgcgatac ccgtaaccgc cgtcggcagc
    16381 gcaaacggca aatcaaccag cgcgttcgcc aggcccttgc ccggaaattc atagcgcacc
    16441 aacacccaag ccaccagtgt gccgaacacg acattggtca gcatcgcata aaacgacatc
    16501 cgcaaactca gccgcaccgc cgccagcacg ttcggctcgg caatcgtgtt ccgaaagccg
    16561 tcccagccga tttccgccgc cttcgccgcc atcatcgcaa acggcaaaac caccagaagc
    16621 gacaggcaca atacggtcag accgaggctg agtttgaagc cgggcagtac gccgggtgtt
    16681 ttgagcggta acatggtcgg taagggtgga aaataaaggc attactttaa caatgccgcc
    16741 tgaaaaacgg aaaaaatgga aagtttggtg caaagacgaa tttgttataa agcggttggc
    16801 agtttcttaa gcgggcgcga tgttctaaaa taacgctttg ttttgacgga aaaccatcat
    16861 ataaaggaac acttatgcag attttatctt ttcaggcgga cattgcggaa cgtatgctgg
    16921 aaggtacgga aggcgagtcg gtcaacgaaa acgcacaatt tgtccgtacg gacaacggct
    16981 attggattgc gtggcatgaa ggcgtggcgg cactgcttgc gcccgatacg ccgccgggca
    17041 ttccctgttt ttgggtggaa ggggcggaaa gccttgaaga gttgtgcgcg atggtggaac
    17101 gcggcgagtt tgacgaagtg gaagagtttg acggcgatga cgacgcgtgg ctcgaagcgg
    17161 ctaaagattg cggacaccac ggcgacgctt gcgcctgcgg acattaaagg catggcaggc
    17221 ttgccgcaag gggtgcaagg ctttgccgtt ttttaaataa tcggcggatt gcatctgttt
    17281 gcaatgggcg gaacgcttcc ctttgtattt tattgaataa aaattatctt ggtattataa
    17341 taaggcagca tcaattattt tgggactgca acaaacgcaa agcattgatt tgcgtttgtt
    17401 gcaaacttat tattatagca ggttgcggcg cggacttgat gggaattgtt tatagagcat
    17461 gatagtctga aacatcatta attaaaaata ccattaatcc gatttatatt tatttcaatt
    17521 tcaatggaaa aacatcaatg acaatgattt taagcatttt aagcctgttt tttatcatta
    17581 gactgttatt tttagccgtc tccattaaac atgaaaaagc cttgattgcc aaaggggcga
    17641 aacaatacgg aaaaaccaat tccacggtgc ttgcggcagt tcatacgctt tattatttgg
    17701 cgtgttttgt ttgggtatgg ctttctgaca ctgcttttaa tggcatatcc ttgattggta
    17761 cactgacggt gatggcttca tttgtgatat tatcattgat tattaagcag ttgggggaga
    17821 tttggacggt taaaatctat attttgccaa atcatcaaat taatcgttcg tggttgttta
    17881 aaacattccg ccacccgaat tattttttaa acatcatacc cgaactgatt ggcatcgcct
    17941 tattatgtca agcgtggtat gttttattga ttggcctgcc catttatttg ctggtcttat
    18001 ttaagcgtat ccgacaagaa gaacaggcga tggcaacact tttttaaccc gtttcatcaa
    18061 ttaaaaatat atcgacaaag agaaccgtct atcaattaaa tccattctta tcattggggc
    18121 aggcttattg tcattgggca agccgtgaca aaagcccgaa cctgcaaaac ttatctataa
    18181 ggctgcatca gtgcgtattg aagccccgcc ttttttgaca aaaaaatgcc gtctgaaggc
    18241 ttcagacggc atttgttcgg tttggcaaag ccggcggctt atgcggcaaa acgtttggcg
    18301 acttcgtccc agttgacgat ttcccaaaaa cctttcaggt agttggggcg gctgttgcgg
    18361 tagtcgatgt aataggcgtg ttcccacaca tcgcaggtca gcagcggcgt gttttcagtg
    18421 gtcagcggag ttgccgcgtt ggaagtggaa accaaagcca atccgccggc aggggttttt
    18481 accagccacg cccaaccgga tccgaaagtt ccggccgcgc aggcattgaa cgcttcttgg
    18541 aatttttcga agctgcccca tttcgcgtcg atggcggcgg ccagttcgcc ggcaggcttg
    18601 ccttggcctt tgggcgtgaa acccagccag tagaaggtgt ggttccaagt ttgagccgcg
    18661 ttgttgaaca cgccgcccga agattttttc acaatctctt ccaaaggcag gttttcaaat
    18721 tcggtgcctt tgatttgatt gttcaggttg gtgatgtagg tttgatggtg tttgccgtag
    18781 tggaattcca aagtttcttt gctcagatgc ggggacaatg cgtccggccc ataaggcagt
    18841 tgcggcagct tatgttccat tttgtgctcc taatattgtt ttaaatgttg tgttttggca
    18901 gtgttgctgc aaacgaatcg gcggctcacg cattctacct gttttgagtg atggaaacca
    18961 attaaacctg ctttgcgtta taatagaaga ttgcaatttc ggcatgacag attggatgta
    19021 ccatgaacga ttacgcagct atgccgcctg aaggccgtga ggtcggcgca ttgtcgctgc
    19081 ccccgcattc gatggaggcg gaacaatccg ttttgggcgg tttgatgctg gaaaatccgg
    19141 cttgggacag gattgccgat gtggtttcgg gagaggattt ctaccgccac gaacaccgcc
    19201 tgattttccg atccattgcc aaattaatca atgagggccg tcccgcagat gtgattacgg
    19261 ttcaggaaga tttgcagcgg aacgaagaat tggaagcggc aggcggattt gaatatctga
    19321 ttacactggc gcaaaacacc ccgtctgccg caaacatccg ccgctacgcc gaaatcgtac
    19381 gcgagcgttc cattatgcgc caactcgccg aagtggggac ggaaatcgcc cgcagcgcat
    19441 acaatccgca aggcagggac gcggggcggc ttttggatga ggcggaaaac aaagtgtttc
    19501 aaatcgccga aagcaccgcc aaatccaagc agggcttttt ggagatgccc gatttgctga
    19561 aagaagtcgt acagcgcatc gatatgcttt attcgcgcga caatcccgat gaagttaccg
    19621 gcgtaccgac agggttcatc gaccttgata aaaaaacctc gggtctgcag cccggcgacc
    19681 tgattatcgt cgccggccgc ccgtctatgg gtaaaaccgc gttctccatc aatattgccg
    19741 agtatgtcgc cattgaaaaa catttgcccg tcgccgtttt ctcgatggaa atgggcgggg
    19801 cgcaattggt aatgcgtatg ctcggttcgg tcggacgctt ggatcagagc gtcctgaaaa
    19861 caggcaggct ggaagatgaa cattgggggc gtttgaacga ggcagtcgtc aaactttccg
    19921 acgcgcccgt gtacatcgac gagaccccgg gcctgactgc gctcgaactg cgcgcccgcg
    19981 cccgtcgtct cgcccgtcaa tttaacaata agctgggatt aatcatcatt gactacctgc
    20041 aactgatggc gggatccggc cgttccgaca accgcgcttc cgagcttggg gaaatttccc
    20101 gttccctcaa agccctggca aaagaattgc aagtccccat catcgccctg tcgcaattga
    20161 gccgcactgt cgaacagcgt accgacaaac gcccgatgat gtccgacttg agggagtcgg
    20221 gggcaatcga gcaggatgcc gacctgatta tgtttatgta ccgcgacgaa tactacaacc
    20281 aggactcacc catgaaaggc cttgccgaat gtatcatcgg caaacaccgc aacggtcccg
    20341 tcggtaaaat cttcctcaca tggacgggac aattcaccaa attcgacaat gctgcctatg
    20401 ttcccgagga ggcaaagata gaagattaaa tggctatata aaaatttatt aggcggaatc
    20461 aggcaaaatc gtttaaaatc atgcttagag attgccctaa aaaataaaac gcggtcttga
    20521 ggcatttttg cattcagccc gcatataatt gaaaatatag tggattaaca aaaatcagga
    20581 caaggcggcg
//
LOCUS       DAXDZG010000035        20529 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXDZG010000035 DAXDZG010000000
VERSION     DAXDZG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.035"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(56..670)
                     /locus_tag="ACFB78_001115"
     CDS             complement(56..670)
                     /locus_tag="ACFB78_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269112.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            complement(723..1793)
                     /locus_tag="ACFB78_001116"
     CDS             complement(723..1793)
                     /locus_tag="ACFB78_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269113.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMASWFKS"
     gene            complement(1908..2357)
                     /locus_tag="ACFB78_001117"
     CDS             complement(1908..2357)
                     /locus_tag="ACFB78_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC1269114.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            complement(2359..2670)
                     /locus_tag="ACFB78_001118"
     CDS             complement(2359..2670)
                     /locus_tag="ACFB78_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC1269115.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            complement(3074..3538)
                     /locus_tag="ACFB78_001119"
     CDS             complement(3074..3538)
                     /locus_tag="ACFB78_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269116.1"
                     /translation="MIENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLHTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLK"
     gene            complement(3627..4406)
                     /locus_tag="ACFB78_001120"
     CDS             complement(3627..4406)
                     /locus_tag="ACFB78_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269117.1"
                     /translation="MPNWCSNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDEPFAKRIAENIRQYGHPNWYDWR
                     LANWGCKWDVNPDCTFVTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEE
                     GMWFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            complement(4977..5549)
                     /locus_tag="ACFB78_001121"
     CDS             complement(4977..5549)
                     /locus_tag="ACFB78_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269118.1"
                     /translation="MPITCGLLAAGAETLLEIVMSIKVDNILLIEGTKESVAEAAKLI
                     LFRSPVHPEHWAFDFNRILPLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIE
                     ICFLTGLAPPFGIYRKLAEIFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMA
                     DIRKFGIAEFDIWYEGLSDRLESDGYLVED"
     gene            complement(5584..6165)
                     /locus_tag="ACFB78_001122"
     CDS             complement(5584..6165)
                     /locus_tag="ACFB78_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269119.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRHVLREVVTGWLNREILPELYNV"
     gene            complement(6401..6742)
                     /locus_tag="ACFB78_001123"
     CDS             complement(6401..6742)
                     /locus_tag="ACFB78_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269120.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            complement(6819..7940)
                     /locus_tag="ACFB78_001124"
     CDS             complement(6819..7940)
                     /locus_tag="ACFB78_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC1269121.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTSGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            complement(8011..8235)
                     /locus_tag="ACFB78_001125"
     CDS             complement(8011..8235)
                     /locus_tag="ACFB78_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269122.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGGTFD"
     gene            complement(8788..9045)
                     /locus_tag="ACFB78_001126"
     CDS             complement(8788..9045)
                     /locus_tag="ACFB78_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269123.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            complement(9293..11776)
                     /locus_tag="ACFB78_001127"
     CDS             complement(9293..11776)
                     /locus_tag="ACFB78_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC1269124.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRFVDRQD"
     gene            complement(11872..12363)
                     /locus_tag="ACFB78_001128"
     CDS             complement(11872..12363)
                     /locus_tag="ACFB78_001128"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1269125.1"
                     /translation="MPLPDFRIRLLDSADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRIGKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            complement(12365..12649)
                     /locus_tag="ACFB78_001129"
     CDS             complement(12365..12649)
                     /locus_tag="ACFB78_001129"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC1269126.1"
                     /translation="MFMTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAR
                     EAAQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            complement(12882..13256)
                     /locus_tag="ACFB78_001130"
     CDS             complement(12882..13256)
                     /locus_tag="ACFB78_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269127.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARFSSFENSVDARISSFENSVDARFSAIDTK
                     INAIYWFIGITTTVAVAAAKFL"
     gene            complement(13482..14063)
                     /locus_tag="ACFB78_001131"
     CDS             complement(13482..14063)
                     /locus_tag="ACFB78_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269128.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            complement(14073..14588)
                     /locus_tag="ACFB78_001132"
     CDS             complement(14073..14588)
                     /locus_tag="ACFB78_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC1269129.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKKPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            15417..15740
                     /locus_tag="ACFB78_001133"
     CDS             15417..15740
                     /locus_tag="ACFB78_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269130.1"
                     /translation="MTYGREGHSGKLPKGQPSNSCFKSNKNKKFGLLKNCIFLLMLTI
                     DMSIDKKTILLKAKSLRGLTLCFTIIQELLGKLNRRFDRGLIFQAMSWKTKNGEARKL
                     PTILK"
     gene            complement(15761..16210)
                     /locus_tag="ACFB78_001134"
     CDS             complement(15761..16210)
                     /locus_tag="ACFB78_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC1269131.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(16474..17028)
                     /locus_tag="ACFB78_001135"
     CDS             complement(16474..17028)
                     /locus_tag="ACFB78_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC1269132.1"
                     /translation="MKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDRTINCKR
                     ADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYAMQEWAR
                     QSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELEQMAKYY
                     NVGGIGIYNSFVHLDTGRVRHWRG"
     gene            complement(17150..17689)
                     /locus_tag="ACFB78_001136"
     CDS             complement(17150..17689)
                     /locus_tag="ACFB78_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269133.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            complement(17704..19743)
                     /locus_tag="ACFB78_001137"
     CDS             complement(17704..19743)
                     /locus_tag="ACFB78_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC1269134.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            complement(19935..20366)
                     /locus_tag="ACFB78_001138"
     CDS             complement(19935..20366)
                     /locus_tag="ACFB78_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC1269135.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
BASE COUNT         5652 a         4811 c         4445 g         5621 t
ORIGIN      
        1 tgtgattggc agcatttcag ctaccaatcc tctttcttat atgatacaca tcccattatt
       61 tataagttac ttggatgctg tcacttgtaa tccaacgttc gctgcctctg ttcggctgaa
      121 ttttaattaa gccattctca cctttttcta agaccaaggc agacccatag gaagtggaga
      181 tttttttacc gggttgtaca tccgtaactt tgctgcggta ggcttgttcc cctgtatatc
      241 catcagagaa tgcaaaattc gtatagtttt ctctgtacca ccatcccatc cactggactt
      301 gtctgccgtt gatacagaaa atatcggaag tcagggtttc aaccgtcccc tgatgatgtt
      361 cgcaatatgc tttgaaagtt cctactgcat cctcaaccgt tttttcatgt tcggattcgg
      421 atcttgcata gaattttcgt tcaatagttc ccgtactttc aaaaatcttg ccttggatga
      481 tacggccaag tcccgccaat ttactgtctt tcatccaatc ttgtacaaac ttgggatagg
      541 gtttgtctaa agaagcttcc aataccccgt caattttggc cggctttgaa gcaagattca
      601 agaaattacc ttcagcttgg gcatttgaaa tggccatagc tgctaataat ccgaacataa
      661 tttttttcat tgcaatattt tcctcatctt attgaaactt agggtttgtt ctaaacagtg
      721 gtttatgatt taaaccaact ggccatgcgc tgcagaatcc catgcttttt cggaatttcc
      781 cttttcccgg agtgagcttg gaaattctga tgttctaata aggctgcgta ttgggcggcc
      841 aaagccgaat attcagcttc caataaaaca tgtttattcc tcagcgcctc aaatttttcc
      901 tgtatttgct ctgcttgttg ttcatcggcc ttaatttgat tatgcaacat caaggccgtc
      961 tgaaaatccg cactcaaagg attacggagt agggtgcaga gaatttgttt ccgaaaggtg
     1021 ttccgaagtt tctctacatg gcgatttatg cggtttttga tttcttcatt atcataattg
     1081 agcagctcgt cttcatcaat atacaaaaat gaacccttat ctgcccgtat ctgaaccacc
     1141 gggataccta ttttcgataa ggctaaacat ttaggcgcat tggaagccaa ccgtgatgta
     1201 tgccaaatct caaccgccaa ttttctctgc caccttatct cgtgcctgag cagggaagtg
     1261 ttacccggct gatcaaattc aagcaaaaca tcaatataaa aagttttgcc atctatatct
     1321 atccgatatt cggataatgc acggttcagc ctaatagtga caggcggttg ttgctcggcg
     1381 tgttctgtgc aatttagaac agtcgtcagg ccgccgggta agttggatag ttcgttcagc
     1441 acttgtttgc aaagcaagtg ggttagcgat tcaggggcgg aagcaccacc ggagtcggct
     1501 tcactgtaat agcgaaaaaa attggtcgcc ttcctcctgg tcaaaaccat tttctttctt
     1561 gtaccgggac gatgacggct gtagtagatc attccttccc ggtgttttag ctttgcttcc
     1621 agcgcagtta cccattcatc accatccgga ctcgtataag caataatttt caaacgatta
     1681 aaatcctttg ggaagaaaag catcatcaga ataagacaac aataaaatcc tatatatcct
     1741 gtttgccgcc aaagggcaat ttttcttccc ccttttagat ttgtccggct catatacgga
     1801 catgggcaac attatagata acagcaatta ttattacaat gttgtaacat tcagtctaat
     1861 actgtatttt gaccatgaga atcttctaag ggctgttttc ttttgctcta ttcctttgat
     1921 actacttcaa gcagtgcagt ccaatctgaa ggaggatttc cgttccccag catttttttg
     1981 aacacagtat atgcatcatt tttgccgcca taagttcttt tcgtacccga gtcgtttacc
     2041 catgcgagaa tgatgatttt actgtttgag tcgtaacgga aaaacaatcg gtatctaccc
     2101 ataaacttgg cacgtcgcca aaatttgtat tccgggccga gtgtcttgcc aagccgatat
     2161 tccgggctat ccggattaag ggcaatattt tctatttcgc ctaagattcg ttccagcagt
     2221 ttggcctctt cggatctact ggatttttcg gatcgtttct ggctttctat cgtagtggcc
     2281 agtctgtcta attgctgcgt aaagcattcg tgttcgtaaa tggtatatcc gtttatctgc
     2341 ataggggaat caggcatatt agtctttcat atctgtttcc caagtcagtt ggcgaaggcg
     2401 gttaaagcgt gattgagatg tgggtttgat ggaattcgga ttttttgtca tagaattttc
     2461 caaaaaatgc aggaattttc caacaactgg atcatgctca aattcttctt cctcagtatt
     2521 gtttttttct agcaatacct gtccgccgcc taagacggaa aaacggattt tgtcctgttt
     2581 ttccagcccc aacgccttgc gtactgactc gggaatggtt gtttggtttt tgctggtcat
     2641 agttgacact gcatcaaaaa taatcttcat ggtctctttg ccctgccttc attcaaattt
     2701 actgttgcta cttgcgactt caatgtccta gttacgcaca tcttctactt aacggcttgg
     2761 gaaattattg atattgttaa aaactgctta aattaaagaa ttctgtttat tgtaaggaaa
     2821 tttccttaca gggtcaatac ccaacccctt cgacctgcaa gggcgtttat gaatacccat
     2881 ttactgactg gcagcgatga cacgcttaag tgagggagca ggcgtaggcc ggaggtaatt
     2941 cctgttcccg agctgtcggc ggcgagaccg ggggattggg ggcagagacc ccaaaaatat
     3001 cagagtatgg gcacaaaaaa acggccgcat tagcggccgt tggttttgaa aaagattatt
     3061 tgaggcacaa atgctacttc aaacaggcat cgagcttgga ttcaggatca cactctccga
     3121 aaaactcatc caggaacgct gataagtcca gttcatacaa cgtccccgtc cagtcgttat
     3181 aaaactccaa ataataagag aacgatttga tagggaaagg ctttacatcc aatctttcgt
     3241 atctcaactt tccttcgaaa ccgaacgaat acgcactgca ttttgtgctg atgccgtgca
     3301 aaatgccctg gttttcgttc aaaagtctaa cagcagtgtg aagggattca acatcgatgc
     3361 tgacttctaa gtcgggtacg gtaaagtcat actcgtccgt ctgccaaatg gtatttaatt
     3421 cgtcactacc ttcgggcagg ttaaggtaaa ggaaacgttt gccgttttcc tgttttagga
     3481 tgaatcggcc ttcgcatgaa gcctgagtga tctctttgtc aatctgattt tcaatcattt
     3541 ctaatctcca caaaaaagcg ggggacaacc ctgcgggttg tccccgcagg gtgagttggc
     3601 aatacgtcta acgaaatcta agttctttaa tcttcgtcat cgtattcgca tccaaagtgc
     3661 tcggtagcga agtcacggac tccatcatct gtgcacggat aatcgaacaa tgctccttca
     3721 tgaccttcat aggttcctgc aaaccacatt ccttcttcca ggtatttggc ttcaaattct
     3781 acgtcaggaa aacgtttggc gagttctcta taaatccctt ccggaggacc ccaggcagtg
     3841 tcacagctga tccggatgcc gctttcccca actgtcacaa aagtgcagtc ggggtttacg
     3901 tcccatttgc agccccagtt agctaaccgc cagtcatacc aattcggatg accgtattgg
     3961 cggatgtttt ccgcaatacg cttggcaaat ggttcatcgt aacggaaacg agatgcgact
     4021 tcagagttag cttcaaaatg gagaaatact tcaccaacag tcagattgtc ccaatcaaat
     4081 ttttccgaaa aatatagctt taattcagag aaaagttcat ccggcaatct atattgaacg
     4141 gaccaaaagg atttatcgcg ctgttccagc atttcccgtt ccaaattgat caacttcttt
     4201 tcaggttgtt gagcaatttg aaaccaggtc tgctgcatgt aactatcagg cacttcagga
     4261 agaatcagct cctccggcat gggtacaata tgctgaaagc tgaattgata ccccttgtcg
     4321 gttttgatca gaatttgctc cgccacttcg gcgatacgtt gaggattgcc ttgaaaatga
     4381 aggctgttgc tgcaccagtt gggcatagta gtctcctgag caggaatccc catccctgcg
     4441 gataaggaaa cccgcaggtt gataggcttg cgcccgggtt gataaaaaag ccgccaccgg
     4501 aaaccggcgc acatttcaga cgacaggtgc tttagataaa aggcaaagaa accgaaacgg
     4561 taactttgcc tttgggaagc ggaacgacgg ttccgccggc ttgatgaaaa atgtgcttaa
     4621 gcacggttat tttttggggg ttttaagata gattctcgca acaataacaa gaaccaggcc
     4681 gactgcggcc atgacccaag ttccggtaac gaagtccatt atttgtcctc cagtttagag
     4741 atgtgaatgc ggataaggaa tgaaacatac agcatttcaa acgacaggag caccaatcct
     4801 gccatggcaa gattcttcat ctcctcacta tatccgttac tccagaccac cacaccgatg
     4861 acggggaata catccccttc agggttgata aaagagccgt gcatccgaaa cggatgtgca
     4921 tttggttaac ggcggtatgc gggcggtttg acggactgcc taccgtgtaa catagtctaa
     4981 tcttctacca aatacccgtc gctttcaagt ctatcggaca agccttcgta ccaaatatcg
     5041 aattcggcga taccgaattt acggatgtcg gccatctcgc aaagacagtg gtaaagcacg
     5101 ctgttacggt attcgtagcg tccgccgaaa cttccgtact ggttgatgta tttcgctgta
     5161 aaacatacat ccaagttggc gaagatttcc gcgagtttgc gataaatacc gaaaggcggt
     5221 gccaatccgg ttaaaaagca gatttcaata aaaccctctc tttgtgtaac aaaggtttcg
     5281 gtgggatatg gggaaaaact gctgccccat gcctcgattc tctgtttggc aaggtcagac
     5341 gtgatatttt cgggtaaagg caaaatccga ttgaaatcaa aagcccaatg ctcaggatga
     5401 acgggggagc gaaataaaat cagtttcgcc gcttcggcaa ccgattcttt ggttccttct
     5461 attagaagga tgttgtcaac tttgatagac ataactatct ccagtagagt ttctgcccct
     5521 gcggccaaaa gaccgcaggt aatgggcatt tacccagttg gaaaagaagc acggcggtta
     5581 tgcctacaca ttgtacagct cgggcaggat ttcgcggttc agccagccgg tgacgacttc
     5641 gcgcagcaca tggcggcagc catcagtcag aacatcagat agactgaaac cgaactcgta
     5701 cagccccagc ccttcattcg actgccactc gatgatggca ttgatgtggc ggcggaaaat
     5761 ccaggcaatg tcttcatctg ataaatactg caatgccccc tgaaaaataa ccattttgtc
     5821 aggacggttg aaatactgcc gataggcagg catcagatag cgcatgtcgg caacggcgga
     5881 tacgccttta ccggcgtatc cgtcctcaag gtaagtgacg gctgcaacgg catcaccatt
     5941 gctactgctg atgcaaacca tgtcggcatt agcctgtttg gcaaactctt cacaggtttg
     6001 ggcaaagacg gccaaatcag cccctaatcc aaatcttttc agttcacgtc taatagcggc
     6061 catgacacca tccgtgtgag cggtcaaaac tgcaccgttc aatttagctg ccgcagctgc
     6121 cgattcttcc cgatcgggat tcatccatac aataaagttt tgcattgtgt tgctccttaa
     6181 aaagaaaacg gagaacacag tccgtaggga ttgtgttccc cacggggttg ataaaagaaa
     6241 atgccgtaca tccataagga tgcgtgtcat gtttaacggc aggacacaag acagataagg
     6301 tctgccagcc tgtaaagaca ggtattttca gacggcctta ctaaagccgt ctgaaaatac
     6361 ctgcccgaag gcaggtgaga tatataaaaa gattacaagg tcagtaatct tctgggaaca
     6421 ggagtgtggt aaggcttctg tcccattctg taacaaccca aattttttgg tccccaatct
     6481 tgtaagaaga cattattctg ccttcttcac ctggcttgag tgccagctga ttcggcttgg
     6541 catcctcagg aggaatatca ccccatatgc catggcggtg ggcctcaagc aatatttggg
     6601 cataaagact gttctcatac agttgtgcag gcaggtgctc ttttgcccgg taatctactg
     6661 ttgccgtcat agacactcgg cccaggtcaa acagagaggg ggtatcacat gcggccggag
     6721 ctgccgaatc aagttgttcc attaggcgta tccttaaaaa aaccggatac acccctgcgg
     6781 gaagtacccg gcagggttta aaaattgaat aggccaatct atttgattgt caggactcgt
     6841 ccaaaatccg acgatccttc ggtcaaatca attgcgcgta tgcttggaga aaaccggtca
     6901 gttgaagtaa tcgtgaccac aagcctgtcc ttcttgccgt actcttccga tttggtttcc
     6961 actttggttt tatgggttga acctttaacc agcagctttc ggccactggt tgaggtgaca
     7021 acgccattaa cttgccctgc ggctaatgct aaagcagcat gccactgacc cagagcacat
     7081 aaaggccggc gtttttcggc tataacggca ctggtgccga aatattgcga aaatgatggc
     7141 cataacgtgc gggattgctg ctgtcccagt tcacgttcca atatatcctg ctccaattta
     7201 aagcttctgg ggcgaaagac cttattgggt gcaataggca cacagtatgc aggcataccg
     7261 gccggcaaag tctcaggggc attttcaaaa tcaagcagga gcctttgttg ggcatccgcc
     7321 tgttttttgc tgatagaggc tttgttcttc ggtcggatgc cgaatataac caactgtttg
     7381 taggtatccg ccgcagccct tatcatacgg acatccgtac agtgggtagc gatttcgccg
     7441 gttagatgct ctgtcagggc ggcttccgga acaatcatta ccaacactcc gccgggctgc
     7501 aaagtaccga aggtacgtgc gaagaaaagt tcttcaagcc gtttggttct ttgattagac
     7561 agattgtctt tggcggcaaa gccgtaagga ggattcagaa acaggaggcc gactgccgaa
     7621 ccctgcatgg tgcagttttc tacatcggat ttgatgacac tggtcagtcg gctcattgcc
     7681 tcttgggcac ggtctttctc cagttcaatc ccgtaagttt gacaacttat tttttccgat
     7741 tgcaaagtag aagctaaaga agccagggct tctcctgttc cgcagcaagg atcaaatatg
     7801 cgtaacaaac ctccggcaga tgccggaata tcaaggcaag cggctatacg cgacaatgtg
     7861 gcctggtcgg tggggaagta tccgttgtca cggtggttat gcgcgacacg ggggtgcatc
     7921 agatggtttt gagtttccat attggtctcc attcaaataa aaaacggaga catgccgtgc
     7981 gggacagtcc ccgcagggtt aaatcaaagt ttaatcgaag gtaccgccac gttctatatg
     8041 acgtaccatt gttttaaaat tcagcaatcc acgcggatcc tgtcccggcg agccgggtat
     8101 cggaattttt cgatcggtcg gtaggtgctt caatgcgctg tgcttacgac cgtttttggg
     8161 gaggaacatc cattctcccc ccgataccag ttggcgtata aaaaattgat aatctttgtt
     8221 tttgctatat ctcatttctt ctccgataca aaaataggtt ttcctcctgc aaatggttaa
     8281 aaatacacgc atacgattaa aaaaaacgta accgcatgac ttccaagaaa aatcccactg
     8341 ccgtacagac gtacggaaca gcgggattgc ggcttcaagc cggatttatc taaaaaggaa
     8401 cgtatcagcg ttctatttaa aatgctttgg tattaaatag caacaaagca tttaaaagat
     8461 gggaatacca attaacttga ccggtcatgt gtattagtgt cttgctgaga ctcgccaaac
     8521 tttaccagtt taaatatgcc aaacaacatg acggcaatca aaaatagtaa gctgtaggtc
     8581 atgatcatgg tttttccctt tccttgggtt cctgtttgac aatctttgat tcatatttat
     8641 ccaagtaaaa gataaatact tgagctacat agaatgaacc gcgttaacgg tttttgtgtt
     8701 cttccgacgt acgcgaagtt tcaagctgct tggtaatcca atagccgaaa gcaagcacca
     8761 ggaagagaat aaatagcaga ccgcctatca gcatgacgta tcctttctgt ttaatgaatc
     8821 acggtatttc cgtagtttgt cagcatatac ttgcacaaag taggcaacca aagccaatac
     8881 ggctacgccg gaagcacgtt cggtcaaaga gtatgaattg agccaaaaca acggcagcat
     8941 tgccaacaag gctgttttac ctatatcttc cagcaatttg atccaagatt caatgctttc
     9001 cttgtgaaaa ggaaggtcgg gtttgatgaa tataaaaaat ttcacatcgg tatctccatg
     9061 aaaaagggag atataccgat gccctgtggg gatacggtat atccccacag ggttaaagaa
     9121 agttgaaaaa gagccgtccg tcccgaagga cgtgcgcaat gggtaacggc agtgcgcggg
     9181 cagataaggt ctgccagcct gtgaaattcc accttacggg ctttttcaga agacctcaac
     9241 acgaggtcgt ctgaaaaaac ccactaaacg cgggttagat gcaaaaattg catcaatctt
     9301 gtcggtcaac gaagcgtttg gccaactgaa cttcaactga atcttcagcc tgattcagaa
     9361 tgtccagacc cgaatcgggc atatcaccgg aggttgactg agtaacggca actttttgcc
     9421 ccatcagttc aaggcaaatt tgctgcgctg tatcgctgta accggcgaaa tacacttcca
     9481 cgtcttcctt ctgaccaata cgccaagaac ggcgggcagc ctgcatcagg gtataaacgt
     9541 tatatcctgt ttgcaaaaag tagatggttg gaaatgccaa caagtccaaa ccggttttga
     9601 ccagctccgg attacagata acaacctgta cgcctgaagc caattggtct tcaacccagt
     9661 cttcgcgttg atcggctttg accgtcgctt tcattaccgc cgccttaata ccgtgccgtt
     9721 tgaaaatctc tttcagacgg cctgtcgtat cacgggtatc ggtataaaca gtatagacca
     9781 gacatttacg tcctttagcc agactgtctt taacgacttc cagcatgtca gcttctttcg
     9841 gtgagatttt ttcttcatcg aactggggtt cggtatggaa aagaatcttg ttttgtctgc
     9901 gccagtaaac atcttgaggt ttgtaacaac aatccggcca tgacagcaag gcgctcataa
     9961 caacgccggt aagggtattg tcacgattcc ttaatgccac cttcaaatgc tgcctcaaaa
    10021 cactctccat atttttatag accgcctgtt gatcttcagt catttcaacc ggccggaaaa
    10081 cttctttata gccaggtaaa acgccttctc ccaaatcacg cagcttaaga aatacggtaa
    10141 taggcaaaac ataacgcatg atgcccaaag gggaaaatcc cggcgccttg gcggtgcgaa
    10201 cgctgttacg ctctgctttt gagctactga atgcgccatt actgtattca gttccaagat
    10261 gtcggacaat atccttcaaa acgccatgct tacgcataaa cgccatgctt gccgaaccca
    10321 aggtattccc tttgttgtaa ctgaaaccgt cttcaatcat catttgcggc caaagccgcc
    10381 acaatagaaa gaacaggtct tccgcataac cacccatcag cgtaccggtt aagcacagga
    10441 tcttattaca gcaacgtgcc aaaacgccca tggcttgacc ttgagccgta ccgtagttct
    10501 tgtactcgtg accttcatct actaccaaca gcccgaaaaa gttcttcggt aaatagcgtt
    10561 tgacgaactc ggtcggctga tacccgcctc gtcccaggct gaattctgct ttgcctaagt
    10621 ctcggtcgat acgcgccgcc tgacggtcgt cgaataaaaa ttctccgttt tcatccatca
    10681 aattggaaaa cgcctgaatg ttgttttcca agatttcagc cagatactgc tcaccataca
    10741 tcccgatgat ttttgctgcg gttttaggac cgatgccggg tagggtggtc atggttttaa
    10801 gtacgcgatc gtacactgtg cttacaaccc cgtcttcact ctgttccttg cggcacaagg
    10861 tccagagcgg ttccttacac ggtttgccat gatgattgcc tttgcaaaaa cggcggtttt
    10921 tagccagggc gtttttcaca ccaccttcag tgttataagg cttgttttcc tcatccaaga
    10981 tttcacagcc acatttagga cagtaatact cggaaacggc aaaaccctca gaagcacctt
    11041 cagaaggtat ccggcggcgg cgaacggcat acgaaggcct ccaatggtag cccatgcgca
    11101 tccgaacacg ccccatcaca aaaaactccg gaacttcggg tttgcggcca agggcacgga
    11161 tttgcaacag ttttgccaat gtatcggctc cgttaagaat ccatacgcgg gcattgggta
    11221 ccgttttgag gatctctcgt ctccacttgt acaccaaatg aggaggactc aataccaagg
    11281 tgcggtgcaa acctgcttga tgcgcggcag cggctgctgc aaccgacatc atggtcttac
    11341 ctgtgcccat ttcggcatta ataatggctg cgggtttgtc ctctgccacc aattgctgta
    11401 atacggcgcg cacaacttcg cgctgcgccg caaacaaagg gcgttccagg ttatccagca
    11461 cttcttcaag gagaggataa gtttgtccct gatagaccgg ttggttttgc tcagtaactg
    11521 cgttaaagag actatcccca aactcggcaa gaaactgtgt caggggtatg tcggaatccg
    11581 gcagttcagt ttccggagat tcctccggca atacggcagc ttccgtactt ttttgggcag
    11641 tctcagccac ttggcggatg ataaagttga aatcagtagt tatttgattc tgacgttcct
    11701 gcatctcggt cacagtttga tccagccctg caaaatccgg gaggagttcg tctatttttg
    11761 atacggggtt atacatcgta tgctcctaaa aaaaagacgg agcaaacccc ggcgacgggg
    11821 gattgccccg tcagggttag aaaccgctac cgtatggtgc ggaaaaaggg gttagcgaag
    11881 attcgccaaa ggaaaaaata gatgcttggt ggcatcttcg aaagaagtaa agccgaaatg
    11941 cctgtaaaag ccgatggcag cttcgtcttt tgcctccaca atcaacgcat acgcggcgat
    12001 ttctgcttta ccgatacgtt tgagtgcatc agccaaaagt aaagaaccca gtccaaaccc
    12061 gccatatttt ccatctaccg ccaaacggcc catcagcact gcgggaaccg tagggtatct
    12121 gggcagcttc ttacgaaaac cggcgggtat attacttagg gcaactcctg ttgcagccaa
    12181 tgtatagtag ccaagaattt gaccgtcgga tgtaaccgcg acaaaacaag tagcgacttt
    12241 ccgttttacg tcttgcgaaa cctgtttttg cagatagcgg tttaaaggtt cggaagggcg
    12301 ctcaaatgca ttgcggtcat cggcagaatc aaggaggcgg attcggaagt ccggtaacgg
    12361 cattttatct cccgaacaat tcgtcatgtt tttccaatgc tctgctcaat gccccattcg
    12421 gtttggacgg attgagcagg gcttgggcaa actgaagttg gtcacgttcg gacagcatca
    12481 gaatctccgc atcggcaact gctttttggg cagcctcgcg ggcagcactg atgacaaatt
    12541 cggacatact gcgtccttga agttccgcag cgtgtttaag taaagcacgg gtatctgaag
    12601 tgattcttgc ttcaaaacgg caatcggcat atgtggcagt catgaacata ctcctaaatt
    12661 atttctagat tgtacggctg attaccgtac aaaacaagat atgaaagggt attttgtaga
    12721 aaacatcaca tgctccaaag taacggagat atacgatctc catagggatg tatatcccca
    12781 tagggttgag tgagaaatga taaaaatccc accggatgcc ggaacatggt gggattgaag
    12841 gttacagata aaaagctccg tttaacggaa cgggtttaat gttacaggaa cttcgcggca
    12901 gcaacggcaa cggtagtcgt gataccgata aaccaataaa ttgcattgat tttagtatcg
    12961 attgcgctaa accgtgcatc tacgctattt tcaaatgagc tgatgcgggc atctacacta
    13021 ttttcaaatg agctaaaccg tgcatctaca cttttttcaa atgagctgat gcgggcatct
    13081 acactttttt caaaagcgtc cagtcgggca tcgaagttct gggtcatgaa atccagtttg
    13141 ttcaaaacct tttccatgtc gtcctccgtt ttcaaatcga ttaaggctac tatagcagct
    13201 gcatcctgac ggttcaagcc ggacagtacc tcatgctttt cagatagagt aggcataaaa
    13261 tcctcctaag tttgggaata tgcctcccca tgggacgtat atccccacag ggttggtaaa
    13321 attgaaaaat gccgcaacca tacgaatacg gccggataac ctcagcagca atatccatca
    13381 acggaatata cagtccgtca tctgtgtaaa actcttatgt aacagacttt ttcagagagc
    13441 ctttgcaagg ccgtctgaaa aagcccgcta ggggcttcag attaagtatg taagcggccg
    13501 ctttgtagca tagcggtcaa acgcacatca aaatcttccg ggatatcaac tcgtaccgct
    13561 tgaactccaa ctactgcgta ctcttcatct atacgaggag taaaccgcat gatggattta
    13621 tcagcctcaa gtaccgataa aacagcattc tgccaatgtt ccaataacgg tatatcggag
    13681 agttgccgca ccagttgcca agctgccttg tccaagtctg cggtaacatc atccaacata
    13741 acccaacctg ttttactatc gaagttaggt tgaatcagaa tatccgaata caaaaacatg
    13801 tgcgtgaccg aaccataatt aagagtctga tatttcgtca tgtatttgct cagattcaag
    13861 caatgctcgg cagtcgtcag gcgcggacag cttaaaacct cctccggccg tctaaaacca
    13921 agccgttcat tacttacttg cagttgagct tggaaggaca acatgtctgc atcgcggccg
    13981 tgcagagaag caaacatcaa ctgtcggttc caaatgaaga aggcatcggc catcaaatca
    14041 ttgcgggaag tcaggtaaag cattccaacc tcctattgca gacaccaagt ctgcgactgg
    14101 ggccgatagg aataccctgc ctccttcatc attgaacggg ctatgttcag ctcgtcacgg
    14161 tctgaaagga tatactcacc caaactgata tgctcccggt tttgggcgat catttcgccg
    14221 acctgatccg cagtttccgc gtattcaggc aaccggatgc cgttttcagg aaccggatcg
    14281 aacggatcgg atggatcatc ttgcggagaa tccgacccgg aagaaaattc ctgcgcaaat
    14341 tccgccagat gttgccagtc aagagctgca tctaagtaag aggtggcgcc accaccccac
    14401 tcataagcct taactttgag ctgttcaata atgaagtttc cttggtattc accaggctca
    14461 aactgtttca atattggttt ttttacatca aatgttccaa ttgatgtagc taatttagct
    14521 gttttgaaag ggcctcggcg accctgtatg acttgcaccg tcaaggtgcc ggaaagattg
    14581 atggacatgg tatcactcca aaagaaaagg gataccggcc gcgggcagat atcccgtggt
    14641 tgggaaaaaa caaattagcg cttaatagca ctgtaaatct tatccaaata tactttttgg
    14701 gacatacgca gtcccaatct tgtaaaaata cgtttgtcgt tatccaatgc ccgaatagcc
    14761 atattcaacg ctacggaagc gtttttatgg ctatgaacat cgttgacgct cctacaatac
    14821 cgattgaccg ccttttgggt tcgtattttc gaaatacggc catccgcatc aacaagtaac
    14881 aaatgatgtt cttgtatgca tgagacgaca actttccgat gagtatgtcg gtgggtgagg
    14941 atgtcaccga tacgtattct ttccggtagt aaatttgggg gaaggatgac acctgaacgg
    15001 tgaaaagata gatttagatt catggtaata ctccaaaata aagggtacac catgcccatt
    15061 cggggaagtg taccccgatg ggttaaaaga aatctgcttc agaaaaaagc agctgtaccg
    15121 tacctgaaac aggcttgcaa tcaacggcta tgcaatgaca gaaaaaacag tctgtcagct
    15181 gtataaaact cttgaaatac agattctctg agccgttctg caaatatcaa aacgtcttag
    15241 agaacctgca aagcaggttc aaatggggat taccctggct aatgattacc tagtccgcac
    15301 cgacgaagta atcagataat ggtctggata gtaataaaac cgttaattgc gcgcactggt
    15361 tatacggcta tacgcagaca gaagaaacgg tctgtcaacc ggggaagctt acctgaatga
    15421 cctatggtag ggaggggcat tcaggtaagc tgcccaaagg gcagccttca aacagttgct
    15481 tcaaaagcaa caaaaataaa aaattcggat tgttaaagaa ctgtattttt ttactgatgc
    15541 tcacaataga tatgagcatc gataaaaaaa caatattgct gaaagccaag tctttgaggg
    15601 gacttaccct ttgcttcact ataattcagg agttgcttgg aaaattaaac cgccgtttcg
    15661 acagaggctt aattttccaa gcaatgagct ggaaaactaa gaacggggaa gcccgaaagc
    15721 ttcctaccat tcttaaataa tcaaaatatt caatagcttt ctaagaagca tcaggattat
    15781 tttgggtttc atcttgagag gatgattgat accacatatc gccatccact ttaataaatt
    15841 tgaatttcaa caaccgaccg tcgatactta aagtttctaa aacttcgccc gtctcacgat
    15901 tgttagtttc aaattttaaa ggttgaatat caccggcacg gaaaccgacc acgacagtcg
    15961 ggcgttcacg gtaattttta gacagcaatt gcggaaattc ttccaaaagg cgagcaaaga
    16021 gctctttcgc ttgacctccg acgatacgaa gatcgaaacg ggttttctcc ccattatcac
    16081 ctcgggaggc ttggatcgta caagcataat agttggaaga ttttctcttt ccttgaactt
    16141 cacgtggccg gtttaaaaaa ccgataccat ctgcagtcag gttaaagtag ttttgattag
    16201 attgagtcat gatatatctc caaaaaaaca ggggatatat caatcccaaa ggggacagta
    16261 tatccccaaa gggttaagtt gcggctttca cggtagccga actgacttag aagccttaaa
    16321 ggctggctga aaaagaagta ccgtaaattc gacgaatcga atctcattca tgaacggcta
    16381 aatgacgaga cttttaagaa aagtcacaaa ctacaaaaac gattacaata tattcccgat
    16441 aatttcgtat gtcaatattt tttcgtaaaa tctttaaccg cgccaatgcc gaaccctgcc
    16501 ggtatccaaa tggacaaaac tattatagat accaataccg cccacattgt agtatttagc
    16561 catttgctca agctcgccaa tgcccacccc cctcatagta aagtcaactg ctttaccacg
    16621 catatgaagt gagtttctag cagcaccctc aatagtcgca tttcgtcttg gcgttctata
    16681 tgcactattg atcgtaatca cagggtttga ccgtcctgat tgccgtgccc actcctgcat
    16741 tgcatacatc aggtttaaaa gccctacgtc aatttgaacc atggcatttc catctttcgc
    16801 atcacgcatc agccaacagg cattgcggta agcatcaaga tcatatccat tttgttgctg
    16861 aaaaaaacgg atttcatgtt tctcgccagt atctgcccgt ttgcaattga ttgtacggtc
    16921 tcgtgaccaa aaatcagcca tactggttgc cgctactaca tctttaggca ataacaaacc
    16981 ggcagcaccg gtcgtatata gggcgccaac gccgacaaac tttttcaaaa aatttcttct
    17041 atccatctga aattccaaac caatgactcc aatcaaattt taagcaacac tcagtattga
    17101 tatgcaaact tcccagtatg ctggtagagg gcaacatctt tgagcttcct caatctctga
    17161 cagccacaat atccatcgag cacgttttgt tactctgtat attcttagaa agttctttat
    17221 taaaaattga aaaaattgaa tcttggttaa aaaccaattt aacaaaagtg ccaaagaatg
    17281 atatacaata aaacagacga aaaagtccgg atattgcttt tgcaaaatgt gccggatcct
    17341 gtaacatcat gaataatcga tgaaacgctt caggattgtc ggtgttcaac cacagtaaaa
    17401 gcaagacccc cagcggcagg gcagtaagcg caattattgc gggtttcaat aaaaatccta
    17461 gtatagattt aaagatactc caccagaggc tactagaata aaacactcta tattgcaatt
    17521 cttgcagttc gccaggtgaa atactcccta cttgaatgcc attgatgtaa atgaataaat
    17581 ctttattagc gttgtattgc tccttgtaat tatggttttc atctagcaca tactctatac
    17641 aagtatttcc gaatattgaa tcggataatt tttttaccag cttcttcatt tcaaagtcct
    17701 tttttatttg acccattcga agtgtttaca ttcgggaaac ctactacacc cccaataccg
    17761 cttatcaggg ttttccttga atcctctctg tctcagacgg ccttccttac aggtcggaca
    17821 gacatcgtct tcatttcgag cgaccgcctg tttttttgta ccagctgaat ccgtctgttt
    17881 tggcggacga gcttttccta cagcatcctt tttcgcctgc ccaaccagct ctgtgatcac
    17941 tttctgctga taagccataa attcagctaa agttaatttg ccggcttcta tcccagccaa
    18001 agccagctcc catttggctg tcgttatcgg atcggcaatc atagtgggaa tacgggcaat
    18061 cagattacgg cctttttctg tcgcatagat tttgctccgt ttcttttcca tataacccat
    18121 ttcaaacaaa cgatcgataa tggtagcgcg agtggcttcc gttcccaagc cttccgtact
    18181 tttgagaatt tttctgatct gttcgtcttc tacattcttc aaaaagtcct tcaaagtctg
    18241 catctcatcg agtagagaag cctctgtaaa gcgcggtggc ggcgtggtct ttttagtttc
    18301 gggattgaag gaaagcgtgt cgacctcatc gttgactttc agatgtggta gagcaggacc
    18361 ttcatcgatt tgatcgcctt caaacacgtc cttgcccaaa agaaccttcc aaccggaaat
    18421 aaccggtgat tggctggagg ctttaaacaa atgtgaacat gcctcaacct caactacagt
    18481 agagtcatat tcaaaatctg gataaaactg tgcgatataa cggtctcgta tcattcggta
    18541 caccgttaat tcattcttat ccaggactga caaatcaaaa ttactggcct tggtcggaat
    18601 aatcgcatga tgggaatgtt tgtttacctg gttatcgttc catacccttg attgacgttc
    18661 aggatcagct ttttcaatca ctgaaagcat ggatttatcg atagtctgta tcgcggcaaa
    18721 tacatctttc acttcactca tctgcgattt tggcaagtat ctgcaagggg tgcggggata
    18781 tgacgtgatc ttgtattttt cgtagagggt ttgtgcaatt ttgagcactt tggaagccga
    18841 taggccaagt tttttaccgg cttctttttg caaagccgaa aggctgtacg gtaacggggg
    18901 cggggtgctt ttacgctctt tatttgaggt aataaccttg cctgtagatt tctcaagctg
    18961 tttgacaagt gcctttacag cctcaaggtt ggtacagtat ccgtcagtat tacgccagtt
    19021 ttcaggaacc tgccacagtg tagtaaacac tgacttgtct gctgtctcaa aatctactga
    19081 aagggtgtag tagttgtaag gcacgaaagt atcgatctct ttatcccttt ggactacaag
    19141 ctttaaagtc ggtgtttgaa tccgtccgat cgacaaaata tgttctcggc catatccggc
    19201 agaataagca gctgtgtagg cacgactcag gttcatacct accagccagt cggcgcggcc
    19261 gcgcgccaaa ccggcctgat ataggttttc tttatcctta cccggcatta ttgaagataa
    19321 ggcttttttt accgattggg tatctaggcc actcgtccaa aatcgggaaa tctttttacg
    19381 gtagccacag tagtccaata tttcacgggc aataacttcc ccttcacggt ctgcatcagt
    19441 agcaatcact acttcatctg ctttggccaa caagctttta atgactttaa actgcttcga
    19501 taccttcggc ggtacctgca tctgccaaac ttcagggaga atgggcaatg cggagatatt
    19561 tccaaagtca gcatattgtt cgccataagc atctggcgcg gcctgtgtca ggatatgacc
    19621 aaatgcccag gtaacaatag tgtctccatt ctgaaaataa ccatcatgcc tcccgccaag
    19681 aacaccaagc actttaccga tgtccttagc ttgagatggt ttttcacata aaaacaaacg
    19741 cattttaaaa tcccaagctt tgcttcagct gcaaaacaag ccaagatgcg atagtcaaaa
    19801 aagcccaaat tgccaaagca attaagccac cataaataag ggtaacaatg aacatatcct
    19861 taaagtcagc ccataatgca gcaagccaac agttcatttc acgaaaaaag gaagtattct
    19921 tcgtggccat atatttacaa tgggatgtca tcatcagcgt cttcaacgtg attattgtcc
    19981 tgagactgct cacgtttagg acgcaacttg atagcttcta cacgctcagt tggaacaatg
    20041 aatacatctt gaacacgcaa tttacgaacg cgaacttcaa taccgtcacg attggtataa
    20101 gactcgtaac gttcttcacc ggtaacgaaa accggcatac cgacctgtaa tattttatgg
    20161 agatgggcag catcacgatt ccagtattcg caagaaatcc actccgttga aaccacttct
    20221 gatttacctt cggcattgcg tttgaaatta ggtgaggcaa ctgaaaaatt cagtacgatt
    20281 gaggcttcat cgtttttagg gcttgctaca ggacgcagat taaattcagc accgagattg
    20341 ccacgaacaa aaagttgaac tgacatattt ttctccaaaa aaaggattta aaaatatgac
    20401 agaaaagaca gggtaaaata acccaatatg agattaaggg ttctattatc ataactaaat
    20461 aaattcctct tgtcaactag caactattac aaattaaacc ttatgctata tctagctaaa
    20521 atgcccacg
//
LOCUS       DAXDZG010000036        20499 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXDZG010000036 DAXDZG010000000
VERSION     DAXDZG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20499
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.036"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB78_001139"
     CDS             150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB78_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC1269136.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQTIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB78_001140"
     CDS             complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB78_001140"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC1269137.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(3522..3869)
                     /locus_tag="ACFB78_001141"
     CDS             complement(3522..3869)
                     /locus_tag="ACFB78_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269138.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="ACFB78_001142"
     CDS             complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="ACFB78_001142"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC1269139.1"
                     /translation="MTDTPENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(5030..6433)
                     /locus_tag="ACFB78_001143"
                     /pseudo
     CDS             complement(5030..6433)
                     /locus_tag="ACFB78_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(6550..8436)
                     /gene="uvrC"
                     /locus_tag="ACFB78_001144"
     CDS             complement(6550..8436)
                     /gene="uvrC"
                     /locus_tag="ACFB78_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC1269140.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(8491..10668)
                     /locus_tag="ACFB78_001145"
     CDS             complement(8491..10668)
                     /locus_tag="ACFB78_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC1269141.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            10807..11757
                     /gene="trxB"
                     /locus_tag="ACFB78_001146"
     CDS             10807..11757
                     /gene="trxB"
                     /locus_tag="ACFB78_001146"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC1269142.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            11913..12281
                     /gene="rpsF"
                     /locus_tag="ACFB78_001147"
     CDS             11913..12281
                     /gene="rpsF"
                     /locus_tag="ACFB78_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC1269143.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLAAAAEEAAAQ"
     gene            12282..12584
                     /gene="priB"
                     /locus_tag="ACFB78_001148"
     CDS             12282..12584
                     /gene="priB"
                     /locus_tag="ACFB78_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC1269144.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIQARILGRQAEEWQYRQGDCATVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            12591..12821
                     /gene="rpsR"
                     /locus_tag="ACFB78_001149"
     CDS             12591..12821
                     /gene="rpsR"
                     /locus_tag="ACFB78_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC1269145.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            12838..13290
                     /gene="rplI"
                     /locus_tag="ACFB78_001150"
     CDS             12838..13290
                     /gene="rplI"
                     /locus_tag="ACFB78_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC1269146.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(13913..14719)
                     /locus_tag="ACFB78_001151"
     CDS             complement(13913..14719)
                     /locus_tag="ACFB78_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC1269147.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(14720..15466)
                     /gene="pssA"
                     /locus_tag="ACFB78_001152"
     CDS             complement(14720..15466)
                     /gene="pssA"
                     /locus_tag="ACFB78_001152"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC1269148.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            16049..16531
                     /locus_tag="ACFB78_001153"
     CDS             16049..16531
                     /locus_tag="ACFB78_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC1269149.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(16578..17789)
                     /locus_tag="ACFB78_001154"
     CDS             complement(16578..17789)
                     /locus_tag="ACFB78_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC1269150.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            18011..20449
                     /locus_tag="ACFB78_001155"
     CDS             18011..20449
                     /locus_tag="ACFB78_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC1269151.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         4844 a         5164 c         5597 g         4894 t
ORIGIN      
        1 accggtttaa atttaatcta ctatacttgg gtagcgattg ggtttcagac ggcatacatt
       61 caggatttca ccgccttata ccgtctgaaa gacgtttctg acccatccct actacacgct
      121 ttgtccgcaa acgtatcaca ggcaagatta tggaagtcat ccgataccca aattccccct
      181 tcaaactcca ccaacccttc ccgcccgcag gcgaccagcc caccgccatt gcgggcctgc
      241 tcgaagggct ttcagacggc cttgcctacc aaaccctgct cggcgtaacc ggttcgggca
      301 aaacctacac gatggcgaac gtcatcgcgc aaagcggcag acccgccatc attatggcgc
      361 acaacaaaac ccttgccgcc cagctttatg ccgaaatgcg cgagtttttc cccgaaaacg
      421 cagtggaata tttcgtgtcg tattacgact attaccagcc cgaagcctat gtgcccagcc
      481 gcgatttgtt catcgaaaaa gacagcgcga tcaacgaaca catcgagcag atgcgccttt
      541 ccgccaccaa aaacctgatg acgcgcgacg acgtgattat cgtcgccacc gtgtccgcca
      601 tttacggtat cggcgacccg accgagtatc aacaaatggt gctgtccgta aaagaaggcg
      661 acaccatcga gcagcgcgac atcatcgcca cgctcgtttc catgcagtac gaacgcggcg
      721 atttggactt caaacgcggc agcttccgcg tgcgcggcga cgtgattgac gtgtaccccg
      781 ccgaaagctc cgaaaacgcc ttgcgtatca gcctgtttga tgacgaaatc gaccgcctcg
      841 atatgttcga cccgctttca ggcagcctgc accagcgtgt cggacgctac accgtcttcc
      901 cgtccagcca ctacgttacc ccgcgcgaca ccgtattgcg cgcctgcgaa tccatcaaag
      961 aagaattgcg cgaacgcatc gaatttttcg ctcgcgaaca acgccccgtc gagcaacaac
     1021 gcatcgaaca gcgcacccgt ttcgacctcg aaatgctcta cgaaatgggc ttctgcaaag
     1081 gcattgaaaa ctactcccgc cacttctccg gtaaaaaaga aggcgaaccg ccgcccacgc
     1141 tgatggacta cctgcccgac aacgccatca tgttcatcga cgaaagccac gttaccgtta
     1201 cccaaatcgg cggcatgtac aaaggcgacg catcgcgcaa gcaaaacctc gtggactacg
     1261 gcttccgcct gccttccgcc cgcgacaacc gcccgctcaa attccacgaa tttgaaaaag
     1321 tcatgccgca aaccgtcttc gtttccgcca cccccgcgaa atacgaagaa gaacacgccg
     1381 gacaagtcgt cgaacaagtc gtccgcccca cagggctggt cgacccccaa accatcatcc
     1441 gccccgtcgc cacccaagtc gacgatttaa tgagcgaaat caacgaccgt attcaaaaag
     1501 gcgaacgtgt gctcgttacc accctcacca aacgcatggc ggagcaactc accgactatt
     1561 acagcgaact cggcatcaaa gtgcgctacc tgcacagcga catcgacacc gtcgaacgcg
     1621 ttgaaatcat tagagatttg cggctcggcc tgtttgacgt actcgttggc atcaacctgt
     1681 tgcgcgaagg cctcgacatc cccgaagtct ccctcgtcgc catcctcgat gccgacaaag
     1741 aaggcttcct gcgctcccac cgcagcctga ttcaaaccat aggccgcgcc gcgcgcaacg
     1801 tgaacggcgt cgccatcctg tacgccgaca aaattaccga ctccatgaaa gccgccgtcg
     1861 acgaaaccga acgccgccgc gaaaaacaga ttaaattcaa cgaagaacac ggtatcgtgc
     1921 cgcagcagat taaaaaacag gtcaaagaca tcatcgacgg cgtgtaccac gaagaagaca
     1981 gtggcaaagg ccgtagacaa ggcaaaaaca aggtcaaagt cggcgaaatc cacaacgaag
     2041 aagacgcgat taaagaaatc gccaaactgg aaaaagccat gcagcaggcg gctagggatt
     2101 tacagtttga agaagctgct gtgttgaggg ataggattag gaatattaaa gagaatttgt
     2161 tgtttggggc ggagtgattt tgaaatagta atcgcagcct gaatacatag cgtgtgcgcc
     2221 actggcgcgc gctcattgtg ttatcaggaa tccttgaaat catgtacgca gcttgcatca
     2281 cacatcctag ctatacataa acaacaaggc cgtctgaatt ttcagacggc cttttccgtt
     2341 tacaacttta ctacttattc aataactaca cgtgcattca gaggctgtac ggggcggttg
     2401 ttttccgaac cgacggcgcg ggtgaatttt tccgcttgcg cttggtcgat gtggtcataa
     2461 gttttcaaca ccaaccatga tacgccctct gtgcacggcg gcgtggtcag cgaaccggca
     2521 aagcggtagt atttcaaccg tttcggcagt agggtggatg cgtcgaacgg ttggttgagt
     2581 ttcacttttc ctgcggtcat cggcatgacg ttccagatgg aagacaggcg gccgttggtt
     2641 ttgccggctt catacagcac ggctaatact aaaggctgtt tgttttcgtc taagtggacg
     2701 aagtgagctt ccatcgggaa agtgcggcct ttgatttggt tttcgctcgg cacgtggaag
     2761 tggaactgtt tcagggtata ggtgcggccg ttcacggtca gggtattgcc gccttcggga
     2821 taattgacct gaatggtgtg gccgttgttt tccacgtcaa ccatactcgg tttgtaattg
     2881 actttgatgg cgggcagttt gccggaaacg gtttcggtaa tgtttaccgg agattggttt
     2941 ttgccggtgg agcacaaacg gaattcttct gacagattgc cccagctttc gggagagtcg
     3001 tgtccggtat agccccaatg ggtgtgattg ccgtgtgcgg cggcggagaa agcggtacag
     3061 gccaacagca ggacagcggt caggcggggt aaagtgcggg ggaaacgagg cattgtaaga
     3121 tcctttctat taaggagtag aaaaaaggtt gcagtgtgta tgtaaatatt ttatttttta
     3181 ttaaatgata tctatattaa gtgcttaagt caaacttccc ggtctgaaaa ccattagagc
     3241 aatgaaatta aagtgaattt atttttctat aagtcctgct ttatttggat aaccatgagg
     3301 catggctacc tttatccgga aaaatattaa tagccgcaac aaagatattt gaatccgatt
     3361 caaatatcac cagcaagcgt agctcggata cttgtatccg acaaagcctt taacatttcc
     3421 atcgttgcaa tccattgcag caattaccaa aatgtcggat tcgagaatcc gatctacgga
     3481 aaactaaaga ttttatgcgg gctacggctt gcttgttgta tttaatcata tttatttgaa
     3541 ttgaattctt ttattttatt gtgagatgaa aatagtattt tcgatgagaa acccatgact
     3601 aaaatagtat agtctttatc attctttacg aagtaacaat taccatatcc tgcaatattt
     3661 ttatcccaaa aatcacaata tgattcagtg gtaaaatcag ttttagttga tataaaatca
     3721 aaattatttg ggtaagatcc atattgctgg taatatttat ctatattttc tactaagaaa
     3781 tcaatccttt tttctaacat tttaagatta atttgcaata ataaaatgta taaaactata
     3841 ctaattgcta ttacttttaa atatgtcatt ttattttcca gtaaatagaa gtttagttgt
     3901 tttgttaact atgcctgaat gtacacctaa gtcttctttt ggtaacgatg aataattagc
     3961 aagccgtagc ctgcatctcc gccaaccgcg tgccactatc tgcgggcgga ataaacttta
     4021 aaaccgatgt gcaggttaaa acctacaagg atgcgtgcgg tatacacgcg tttttgccgt
     4081 tcaaaccatt gtttcaaaac ttgtattgaa acatcgcccg ctgctttatc ttgaaagaat
     4141 gccgtctgta agcaaacatg cttcagacgg cattttttta tccggcggtc tgttatccga
     4201 tccgtttgaa taccaagtcc caaacgccgt gtccgaggcg tttgccgcgc gcttcgaatt
     4261 tggtttcggg gcggtagtcc ggagtggggg cgtagtctgc cgccgtattt tgcaggctgt
     4321 cgaagctgct gaggacttca agcatctgct gtgcatattc ttcccagtct gtcgccaggt
     4381 ggatatagcc gccggttttg agtttgggca gtagtttggc gatgaagggg gcttgtatca
     4441 gacggcgttt gtggtggcgt tttttgtgcc acgggtcggg gaagaatatg tggatgccgt
     4501 cgagcgagcc gtcttgcagc atattttcga caacttctac ggcatcgtgc cgcatcacgc
     4561 ggatgttttc caaatggttt tcgttgatga gtttgagcag gttgcctacg ccggggccgt
     4621 gtacgtcgat ggcgagaaag tcggtttcgg gcaggcggcg ggcgatttct gcgcttgcca
     4681 ttcccatacc gaagcctatt tcgaggattt taggtcggct gcttccgaaa cgggcatcaa
     4741 gatcggccgg tgttgcttgg tagtcgatgc cgaagctgtc ccataaggta tcgatggcgc
     4801 gttgctgtgc ggcggtcatg tggctttggc ggaggacgaa actgcggatg ctgcgggggt
     4861 gtccggcttg acggtcgttt ggtgtttggt tttcgggggt gtctgtcata atgctttcgt
     4921 gcgcttgtgc aatcaggatg ccgtctgaat gtgaaatgac tgttcggacg gcgggaagga
     4981 aaggggcgat tatacctgtt tcgtttttgc ggggtaggtg taacggtgtt cagacggcat
     5041 tttgccactg ttgcagcagt tcttgcagga cgtttttttg cccgtatccg ttgtttatgg
     5101 cttcttgcag gtagcggcag gctttttctt tgtccgcctg gcaccgtgtc cgctgtaatg
     5161 gatgcaggcg aggttgtaga acgccatact gtttttttgc gaggtggctt gttcaaacca
     5221 tttgcgggct ttgttgtagt cggcggctgt gccctgtccg aagtaataca tactgcccag
     5281 attggtttgg gctttgctgt gtccgagttc tgcggcttct gtgtacagtt tgagcgcggc
     5341 tgcgtattcg ggtgcgcatt gtaatccgta atgcagggct tgggcttttg ataaagctgc
     5401 tctgcccttt gccgcctaat ggcggaatct ttgatgccgt catattgctc gggatggttt
     5461 aacgcgctgt cggatatgag tttctgatag gcggtaaggc tgccggcttc ggctgcctgc
     5521 cggtaataat gccgggcttt ttccgtatcg agggctatgc ccaaaccgta gcggcagatg
     5581 tcgcccggta tccgcaaacc ttcgggatgg cgttcggcgg cggctgcttc ggcatgatgc
     5641 agggcaagtt tgtggtttct ttccagatac ttgccttgca ggtagatgtc ggccagtgcg
     5701 gcatgggcat cggggtcatt ttcctgtgcc gcctgttgga accggtaaac ggcttcatcg
     5761 ggagccgtag gtaggatgag ttcggcaagc ggggtgtagg cgggaatgta tccttgttgc
     5821 gcggcggcgc gcaaatgatt gacggcctgt gccgtgtcgg ttttcgtccc ttgtccgaaa
     5881 cggtattgca gcccgagttg ccaatgtgct tccggcaagc cttgtgccgc cgctttgcgg
     5941 tagagcaggt gcgccgtttc aaaatccggc ggcgtgccgt gccggcagta ttgggcgagc
     6001 aggtattgcc cgcggggatt gttgtttttt gcgcttgttt ttgcccattc cagggcttct
     6061 tcaaaatgtc gggacgcgta cagaaggtac atcaggcgga cttgcgcttc gggcaggcct
     6121 gcttctgcca gaggcctgta aagcggtatc agggtttccg gggcgcgcct tccaattctt
     6181 ggatatatgc caaattgtaa cgcgcatagg gatgcccctg ttcggcggcg atgcggagca
     6241 tttccatacc ttgttcgcgg tcttggtgcg ggctgtccgc gttcatcagg tgttttgcca
     6301 acttgaatgc ggcggcggga tgatctgccg aggccgcttc tgtcagcagt cggcggactt
     6361 cggtaaagtt ataatattgt ttttgagaca ggcgttccaa ggcctgccga tagggtaagt
     6421 cgggattgtt catatcagat cgttttccgt tttgtcgaat catcgttatt cgtcatgaag
     6481 ttaggtttat atcggtatgc cggcctgctg tatcatattt ttacaggcgg tttttgcttt
     6541 ggcggcatac taatgcagat gattataaat ggtttccgcc aatgccttgc tgatgccttc
     6601 cactttttcc aagtcctcgc ggctggcggc aatcacgccg cgcagtccgc cgaagcgggt
     6661 aagcagggct tggcggcgtt tgctgcctac accgggaatg tcgccgagag acgaggtaac
     6721 gcgtgctttg tcgcgttttt tacggtggcc tgtgatggca aagcggtgtg attcatcgcg
     6781 tacggtttgc aataaatgca aggccgggct attaggaggc aggcggaaga gttcgccggt
     6841 aaaaggcagt atgagttctt ccatgccggc tttgcgttcg gggcctttgg caataccgac
     6901 taaagggatg tgcagcccga gttcttccca tacggatacg gctacgccga tttgcccttt
     6961 accgccgtca atcaacacga catccggcca tttgacgctt tcgccgttgg cttcggcttc
     7021 ctgtattttg ccataacggc gcgtcaacac ttcgcgcatg gcggcgtagt cgtcgccggg
     7081 tttggcggtg gtgatgttgt agcggcggta ttgcgagggt tggatgtttt gttcgtcgta
     7141 cacgacgcag gaggcgatgg tggcttcgcc ttgcgtgtgg ctgatatcga aacattcaag
     7201 gcggttgatt ccgtctgaat tcatgccgag gattttggca agttcgtcga tgcggtgttg
     7261 ttggttgctt tgttgcaggt ggcgttgggt aatcgccatt tgtgcgtttt gttccgccat
     7321 tttcaaccaa actttgcgtt cgcctatggt tttggtaacg aactgcatct gcttgccgtg
     7381 ttctccttcc aaagcctctt tcaacgcatc gggaacggga aagttgctga tgatgatgtc
     7441 gggtttgctt ttgcccagat agtgttgggc gacgaaggct tcggcgtaat cttgaccgtt
     7501 tggttcgggg tcgtttttgg tgtcgggaaa gaagcttttg tcgccgacgt gccgtccgcc
     7561 gcggatgctg acccagtgta cgcagacaag gccgtctgaa acggcgagcg cgagcaggtc
     7621 gatatcgttg gggttgttcg ggtttttgct gtcgatgaac tggtcgctct gtataatgcc
     7681 gagtgcttgg atttggtcgc gataacgggc ggcttcttca aattgcagat tcgcggcggc
     7741 ggtctgcatt ttgtgttgca gggtgcgggt cagttcgtcg gttttgccgt tgaggaaagt
     7801 ggcggcttga cgcacgctgt cgcagtagtc ttcttcgctg atgtgtccga cgcagggcgc
     7861 ggtgcagcgt ttgatttggt acagtaggca agggcggtcg cggtgttcga acacgctgtc
     7921 ttcgcaggta cgcagcatga agactttttg caacacttga atgctgtcgc gcacggcgtt
     7981 gctgttcgga tacgggccga agtattggtt gggatttttc agcgtgccgc ggtaatacgc
     8041 catttgcgga tattgatggc cgctgagcat caaataagga tagcttttgt cgtcgcgaaa
     8101 aaggatattg tatttcggcg acaaggcttt gatgaagttg ttttcgagaa tcagggcttc
     8161 ggcttcggaa cgcgtgatgg tggtttcgat gtggcgaacc tgtttcacca tcaacgcgat
     8221 gcgcggcgaa tggtcgtttt tttggaaata gccggacacg cgccgcttga ggttgaccgc
     8281 tttgccgacg tataagacgt tgccgccttc gtcaaaaaaa cggtacacgc ccggcagctt
     8341 gggcaggttt ttgaggaaga gcgggatgtc gaattgttcc ggagaacggt tttctgtatt
     8401 catatcaatc atttgaaaaa cggcttcaga cggcatatcg gcagcggcag ccttatccgt
     8461 tcggaaaagc tgtcgggcgg ttatatgcgg tcaatcgatt tttacccatt tcaggcgcag
     8521 ggcattgccc aataccgaaa ccgagcttgc cgccattgcc gcgcctgcta tgacgggatt
     8581 taaaaagccg agcgcggcga gcggaatgcc caatatattg tagaagaagg cgaaaaatag
     8641 gttttgcttg atgttttcca acgttgcctg cgatatcagc agggcatcgg cgagctgatt
     8701 gaccgaatgc tgcatcagcg tggcggaggc ggtgtgttcg gcaacgtccg caccgccttt
     8761 catggcgaag ctgacgttgg cggcggcaag cgcgggcgcg tcgttgatgc cgtcgccgac
     8821 catcgccacg gttttgccgg cggctttgag tttctgcact tcggcggctt tgtcgcacgg
     8881 actcatatta ccgaaggcgt gtgcgatgcc cagttgtttg gcgacgtatt cgaccgtact
     8941 ttggttatcg ccgctcataa tatagacatc aatattgtgt tttttcagac ggcctatggc
     9001 ttcggcggta tcggctttca acgcgtcgga gagtgcgaat gcgccgatcg gtttgccgtt
     9061 tacagatacg gtaaccgcac tggcgatttc ccaaacgccg tctgaaaact tcggcaaggt
     9121 cagttcggca aattcggctt tgcctgattt caccaaaccc acgccttcca cttcggcggt
     9181 aatgcctgct ccgacaacgg tttgcgcatt ttgtgcagcg ggaatctcca aaccgcgcgc
     9241 ttgtgcggcg gagacgatgg cgcgggcgag cgggtgggcg gcgttttgct cgacggcggc
     9301 ggcgatgcgg tacaaagcgt cttcgtcaaa gccgctgtcg ggaacgtaat aaacggcggc
     9361 aacctgcggc ctgccttcgg tcagcgtacc ggttttgtcc aatacgacgg catcgacgtg
     9421 ggctgcttcc tccattgccg ccgcgtcttt aaaccaaatg ccgtgtttca ccgctttgcc
     9481 catgccgacc ataatcgcgg caggggtcgc cagaccgagc gcgcacgggc aggcaatcac
     9541 caaaacggca acggcgtgca tcagtgcgac cgtccaatcg cccttaatca gccaagtaac
     9601 gataaaagtc agaagcgcga tgcccacgac agttggcaca aataccgccg ccgctttatc
     9661 ggccacgcgc gcaatcggtg ctttactgcc ttgtgcttca gagagcgcgt tcatcatgtc
     9721 gccgagcagg gtttggctgc cgagctgcgc ggcgcggtac accacgctgc cttcggtcat
     9781 cagcgcgccc gccaacactt tgccgcccgc ctttttctct tcgggattgg attcgccggt
     9841 aaggtggctt tcgtccgccc aaccgctgcc gctttcgata atgccgtcgg cagcgatgcg
     9901 ttcgccgtgg ttggtgcgga taaggtcgcc gatttgcact tggtcgatgg gcagttgttt
     9961 ccattcgccg ttgcgttgca cgttgacttg ggtcggcgtg agttttagca gtaagcccaa
    10021 gctgttcagg ctggattttt tggtgcggtg ttccaaaaac ttacccagcg acacaaaacc
    10081 gatcaccatc acgcccgctt caaaatacac atgcgccata ccgtgcgccg catgcgaact
    10141 gaaaaacagc atataaacgg aatacaggta aatcgacacc gtgccgatgg taacgagtac
    10201 gtccatattc gccagcccgc ctttaatgct tgcccacgcg cttttgtaaa acgggattgc
    10261 cagccaaagt tgcactatgc ttgccagtac aaactgccat acaggcggaa tcatccaatc
    10321 gtgccgtgtc caattcagcc cttttagcat catccctacc ataccgataa ggaacgggat
    10381 attgatggtc agcaaaagcc acaaccgcca gccgatatgg tgttctgctt caggttgcgg
    10441 caatgtatct tccgtttttt ccttcgcgcc gtaaccggtt ttctcaatga ttttggcaat
    10501 gtcggcaacc gaggttttgc tgccgtcaaa cgtaacctgc gcttcctcac tggcaaagtt
    10561 cactcccgcc gattcgacaa aatctttttt gttcaacact ttttcaatgc gcgaagcaca
    10621 tgcctgacag gtcatcccct cgatttggaa acggattttt tgttgcataa gtttgattcc
    10681 cgttttgatg ttcagacggc agtcaaggct gcgggatgcc gtctgaacgc cggtatggtc
    10741 aaaatttctc aaacgcgccg ataattcggg tagaatccga aaacttgaca cagcaaagga
    10801 aaaacaatgt cccaacaccg caaactgatt attttgggtt ccggccccgc cggatacacc
    10861 gccgccgtct atgccgcacg tgccaattta aaccccgtca ttattacagg tatcgcgcaa
    10921 ggcgggcaac tgatgacaac gactgaagtg gacaactggc ctgccgatgc cgacggcgtg
    10981 caagggccgg aattgatggc gcggtttctc gcccatgccg aacgtttcgg gacagaaatc
    11041 atttttgacc aaatcaacgc cgtcgatctg caaaaacgcc cgttcgcact caaaggcgat
    11101 atgggcgagt acacttgcga tgccctgatt gtcgccaccg gcgcgtccgc caaatacctt
    11161 ggtttgccga gtgaggaagc gtttgcaggg aaaggcgttt ccgcctgcgc cacctgcgac
    11221 ggtttcttct acaaaaatca agatgttgcc gtagtcggcg gcggcaatac ggcagttgag
    11281 gaggcactct accttgccaa tatcgccaaa accgttacgc tgattcaccg ccgcagcgag
    11341 ttccgtgctg aaaaaatcat gattgacaaa ctgatgaaac gcgtggaaga gggcaaaatc
    11401 atcctcaagc tggaaagcaa cctgcaagaa gtactgggcg acgatcgggg cgtaaatggc
    11461 gcattattaa aaaacaacga cggttctgat caacaaattg ccgtcagcgg catctttatc
    11521 gccatcgggc acaagccgaa taccgatatt ttcaaagggc agttggaaat ggacgaagcc
    11581 ggctacctga aaacaaaagg cggcacggcg gacaatgtcg gtgcaaccaa tatcgaaggc
    11641 gtatgggcgg cgggcgacgt aaaagaccat acctaccgtc aggcaattac cagcgcggcc
    11701 tccggctgtc aggcggcttt ggacgcggaa cgttggctgg gcagccaaaa catttgaatg
    11761 ccgtctgaaa ccgaaatttt cggcggaatg caagtttttc gtaccaaatc cttgattccg
    11821 tcggcaaacc ccaatataat gcctgctctt tgaaccttgc cttctgcttt cgcatggcag
    11881 aaggctgttt ttaccatcca taaggagata acatgcgtca ttacgagatc gtgtttatcg
    11941 ttcatcctga tcaaagcgag caagtgcccg ctatggttga acgttacaaa accatgatta
    12001 ccgaggcaaa cggtaagatt caccgtttgg aagattgggg ccgccgccaa ctggcttacc
    12061 cgattaacaa aatccataaa gcacattatg ttttgatgaa catcgaaacc actcccgaag
    12121 ttgttgagga gttggaaacc gcgttccgtt tcaatgatgc ggtattgcgt catctgacca
    12181 tcaaaaccaa acacgccgtt accgaagcat cccctatgtt gggcggcgaa aaggctaaga
    12241 acctgttggc cgccgcggct gaagaagcgg ctgcccaata attgggattc actaatcttg
    12301 tttcgcttgc cgcattgatt gaaaaggctt tccctattcg atatacgcct gccggaatcc
    12361 ctgttttaga tattatttta aagcacgaat cgtggcagga ggaaaatggg cagcaatgcc
    12421 ttgtccaatt ggaaattcag gcacgaattt taggcaggca ggcggaagag tggcagtatc
    12481 ggcaaggcga ctgcgcaaca gtcgaaggtt ttttagctca aaaaagcaga cgttccctga
    12541 tgccgatgct caggatacaa aatattaaag aatataaagg ttaaacgaca atggctcgtc
    12601 aatcattcaa acgtagaaaa ttctgccgtt tcacggctga aaaaatccaa gaagtcgatt
    12661 acaaacaagt tgatttgctg aaagacttta tctctgaaaa cggtaaaatc attcctgccc
    12721 gcatcacagg aacgaaggca ttctaccaac gccaattggc tgttgccgta aaacgcgcgc
    12781 gcttcttggc actcctgcct tataccgacc aacacaaata attttggagt ttaaatcatg
    12841 caaattattc tgttagaaaa aatcggcggt ctgggtaatt tgggcgacat cgttaccgta
    12901 aaaaacggtt atgcccgcaa cttcttgatt cctgctggta aggcaaaacg tgctactgaa
    12961 gcaaacatga aagagtttga agcacgccgc gccgaattgg aagccaaaca ggccgaaatt
    13021 ttggcagatg cccgagcccg tcaggaaaaa ctggacggtc aaaccgttac cgttgcacag
    13081 aaagccggtg tggacggtcg cctgttcggt tccgttacca atgccgacat tgctgccgca
    13141 atcgttgctg ccggcatcga agccgtgaaa gcaaatgtac gtctgccgaa cggtcctctg
    13201 aaagccgttg gagagtacga agtggaagtg gctttacaca ctgatgccgt tgctaaaatt
    13261 actgttgccg tcattgccgc agccgagtaa ttgaaattat cagatgccgt ctgaaaccct
    13321 gccgattatc agaatgtggt ttcagacggc atttgttttg ttttccgtat attctgcaaa
    13381 aatcgggacg gttgtttttt atttgcctga ggtctttgca gaaatggttt gttaacgaaa
    13441 tttgatgcat aaaatgcgcc aaaaaatatt ggctgtgttt taatatagtg ttgataagcg
    13501 tacctggttt caatatattc aataggatag gtctcttccc aaccgaaaaa ataattgtct
    13561 tttctcttgt aaatagttga aatagattgt caaaatacat cctaaaaaat ttaataactt
    13621 atatgattgc aaaggttgcg gcttgtatgc cggtcattat ggcggaaata ccgtttggga
    13681 ggcagtcgca tcgcatggag tcggcacggc aggcgatgcg ccgatgcctt cgggcgtgaa
    13741 ggcagggaaa tcggcagttt ggaaaaaggc aatggaatcc gcaggtaggt gtcgattggg
    13801 tggtgaaagg caataagtcg ggcgcacgct cagactgcct atccccttgt cggaacggga
    13861 ggcagggcag ctttgtcctg actaattgct tgtgcaaacg gtaagaaggt ttttaaagca
    13921 ggttgtacag catttttccg gcaatcaaaa gcaacataat gccgaaggat tctttgagtt
    13981 tggcagaaga aagtttgtgg gcggttttga caccgagcgg ggcaaaggca atggttgccg
    14041 cgctgaggac ggcgacggcg ggcaggtaaa ggaagcccag cgacccttcg ggcaatcctg
    14101 caatattcag accgttgacc agatacgata ttgcgccgga gagtgcaatc ggccaggcaa
    14161 ggccggatga tgtgccgatg gctttatggg cggggaagcc gcagtggatt aagaagggga
    14221 cggaaagtga accgccgcct atgccgaccc agctcgacat tgcgccgaac agtgtggaaa
    14281 ccgcagtcag tccgggcagc ccgggcagcg ggcgggatgc cgtctgacga ccggtatgca
    14341 gtgttttgaa tgcgacggcg gttaaaaaca ggatgaagaa aatttgaagc ccgaacgcgg
    14401 ggatatattt tgcggagagt gcgccagcga atacgccgaa tatcataccc ggcatcatcg
    14461 caaatatggt tttccagtcg accgcctgtt ttttgtgctg ccccaacata ctggaaaagg
    14521 cggtgaagac catgacggcg aaggatgtgc cgacggcgag gtgttgcgcg taaggatgtt
    14581 gtgccaaacc ctgcaaatca agcacccata aaacgacagg gacaatcagc gtaccgccgc
    14641 ctacaccgaa caggccggca ataaaacctg ccgcactgcc tacggcaagc aggattaaga
    14701 taatgtccca atgccacatt taatccgcct ttctgtattt tttccaaaat cggcgtgccg
    14761 ccataatgta gccggacagg ctgtatccga gaaagaacag gaaaaggacg agcgacggtt
    14821 cccaagtgac cagaagcagc agtaagacgg caagcagcat tccgacaaag gggacttgtc
    14881 tgcggatgtt gatttcttta aaactccaaa aagggatttg gacaatcatc gacaggccgg
    14941 caaacagtgt gatgcctaat gcccaccagt ggacggcggg gaatttttcg acgctgtggt
    15001 tgacccaaat cagcccgaca atcagcgcgg cggcagtcgg actgggtacg ccgataaacc
    15061 agcgtttgtc caccttgccg atgagcgtgt tgaacagcgc gaggcgtagg gcggcgcagg
    15121 cgcagtagat gaaggcgacg gaataaccga ttttgccgaa ctgccaaagc tgccatttgt
    15181 aggcaataag cgcgggggca acgccgaagc tgaccatgtc ggcaaggctg tcgagctgct
    15241 ccccgaacgc gctttggctg ttggtcagcc gcgccacgcg cccgtccata ccgtccagca
    15301 acatagagat gaataccgcg atggcggcgg tttcataacg tccgtgcatg gattgggtga
    15361 ttgcgtaaaa tgcggaaaac agcgcggcga tggtaaagga gttgggcagc aggtagatac
    15421 tgttttgacg cagggagcgt tgcggggtgt tggtcggggt ttccatagct gaagtgtctc
    15481 acggacgtgg agaatcgata ggggattata acccaaacca ttatcgaaca atacgccctt
    15541 taggatgaag ccctcggagg cggtttgttt ttctgatggc ggatgctttg cggggcggtt
    15601 tgactctgcc tgccaccggg tgtgttgttt cggattttgc cggatggata ttgttgccgg
    15661 gcatttctgc gcttgccgga aggtatcgga gggagggcat cggaatatgc ggcgcgccgt
    15721 tcaaaccggg gcggtatttc cctatttttt ggggggtgca ggtttcaaca aattgaaaga
    15781 tattttttgc atttccgacc gtatgctgat tttgaagcgg gataaattat aggacaaggt
    15841 ttttcgcccg ccgcaaaggt tgcccgcaat gccgtctgaa aacaacggct tgccgctaag
    15901 gcggcaatat gaggatttgc gctgccgaaa ccggtttgct acaacccggc agtgaaatct
    15961 gcagggaggg ataggccgat aaggcgggac ttatcttgtt ttttgtttat aatgcaacag
    16021 aatttggagg aatcgtcggg aatttatcat gatgaaaaag atactggcag tatcggcact
    16081 atgcctgatg actgcggcgg cacaggctgc cgatacttac ggctatctcg ccgtttggca
    16141 gaatccgcag gatgcaaacg atgttttgca ggttaaaacc acaaaagaag attcggcgaa
    16201 aagcgaagcg tttgccgagt tggaagcctt ttgcaaaggt caggacacgc ttgcgggcat
    16261 tgccgaagac gagccgaccg gatgccggtc ggtcgtgtcg ctgaacaata cctgtgtctc
    16321 gctggcatac ccgaaagcct tgggcgcgat gcgcgttgaa aacgccgtcg tgattacttc
    16381 tccgcgtttt acgagcgttc atcaggtcgc actcaaccag tgcataaaaa aatacggcgc
    16441 acagggacaa tgcggcttgg aaacagtgta ttgcacgtca tcttcttatt acggcggggc
    16501 tgttcgctcc ttaatccaac acctgaaata aaaaatgccg tctgaaggat gttcggacgg
    16561 catttccata tcgacggtca ggattctttc ggatcgggca gcaggctgtt caacacaatg
    16621 gcgagtacgg cgcacaagcc cacgccgaca aagctgaagc tgcccaattt gagtgtcata
    16681 ccgccgatgc ccgtggtcag taccgagctg acgatgacca ggtttttcgg cagcatcaaa
    16741 tcgactttgg catcaatcag ggttttgatg cctaaagaag caatcgtgcc gaacagcagc
    16801 agcataatgc cgcccatcac gggcatcgga atggaagcca aaaacgcatt gaatttgccg
    16861 aaaaacgcca tgcagacggc aaaaaccgcc gcccaagtca tgatgacggg gttgctgttt
    16921 ttggtaatca tcaccgcacc cgttacttcg ccgtaggttg taaccggcgg gccgccgatc
    16981 agacctgcaa cgcacacgcc caagccgtcg cccgcaaggg ttttgtccaa gcccgggtct
    17041 tttgtgtagt cttttcccgt tacattgccg attgccatga tgccgccgat gtgttcgatg
    17101 gcgggggcga cggcaacggg cagcataaac agtgccgcct gccagttgac ctgaggcgtt
    17161 tcaaaatggg gaacggcgaa ccagggcgcg tgtgcaatgc ttgtcgtgtc caccagtccc
    17221 atcagcagtg ccaaaacata acccgaagcg acaccgatca agatgggaat cagcttcatc
    17281 atcctgctgc cgaaaaccga tacgatggcg gtaacggcaa aggtaaagcc ggaaagaatc
    17341 agcgaatcgg tatagtcgat gacctgtttg ccgtcagcct gacccattgc catgctgctt
    17401 gccgccacgg caacagacag accgatgacc atgatgacag ggccggtgac gaccggcggc
    17461 agcagtttgt gtaccgccgc cagtccgcgc caacggatca gcgcggcaaa cacaaaatac
    17521 ataaagccgg cggcaaacag cccgaacatg gtggaaggca gcccccattc gccgacggag
    17581 tagataatcg gcgcgataaa ggcaaacgaa gaaccgagaa aaatcggtac tttgcgtttg
    17641 gttgtgattt ggaacagcag cgttcccaag cctgcgccca aaagcgcaag agccggattc
    17701 agaccggtca gcaggggaac cagcaccatt gcgccgaatg ccacaaataa aatctgcgcg
    17761 ccggaaacgg caagtttcag ttggttcata ccatcttctt tcaatcggtt caaacggcac
    17821 ggattatact ggtttgccga ggggctgcgt aaaaatacat acgtcgggca aagatatgcc
    17881 gtctgaaagg caacgtctgt ctgttgcagg atgaaagcgg gagaaatgga gtataatccg
    17941 acactttcca tattcaatcc gcaagccgtc ccgaaccctt atttcagacg gcattttccg
    18001 gtaatacggc atgacagaaa aatcccataa aaaaacagca aaaggcaggg cgggaagccc
    18061 gtccccaacg tccgcacgca acaagaaagc cgacaacggc gcgcggggca ataaagtttc
    18121 cgagcggctc aaggcggtca aagagctgca gaaaaccgaa accaaaaagg cgcgtcccga
    18181 acatgtcgtc aacctgattg ccgacgcgct gtggctgatg ggtttggcgg caaccctgta
    18241 tttggcgatt tccctgatca gtttcgatat gggcgatccg tcttggtcgc acagttcacc
    18301 ggttgtggaa gatgccgcca attggggcgg actgttcggc gcgtatgttg ccgatgtcgg
    18361 ctattatctt ttcggctggt cgttctggtg gtggatagcg gctgcctgcg tcgtgctgta
    18421 taaaaatttc cgtctgcacg caaaacagac ggaaaacgag gcatacaacc acaaaatcgc
    18481 tgccgccgcg ctgtttgtcc tgacggtctt cagccccgtc ttggagtatt ttgtgctggg
    18541 cggaaaatat gccgactccc tgcctgtcgg agcaggcggt atggtcggca tacgtgtcgg
    18601 cgcagtgttt gcgtggctgt tggggaaatc gggcagcctg ctgattatcc tggttgttct
    18661 gctgttgtcg ttgtccctgc tggtgcagat ttcatggctg gaatttttga acggtgcggg
    18721 cagggcggtt caaaaccgcc tgagtgcctt atccggcaag gtcatggctt tgagaaaacg
    18781 ccggccgaat accaaaacag acggtgtcga tacccaaaat acacggcgca tggtgaaaga
    18841 agccaaaaac atcactgcca aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa
    18901 atccgtcgcg gtttccgttg cgccgccgcc taaaatccaa ccttcgctgt ttgaagacaa
    18961 tgaagtacag cagaacggcg aataccacaa gcctacattg aacctattgc ggattcccga
    19021 cagcgagccg gtcagcatca atcccgccga attggagcgc actgccgaac taatcgaatc
    19081 caaactggca gaattcggca tcggcgtaca agtcgtatcc gcgacatccg gccccgtcat
    19141 cacgcgctac gaaatcgaac ccgcgcaagg tgtcaaaggc agccaaattg ttgccctgtc
    19201 gaaagatttg gcacgctcta tgtcgctgca atccgtgcgt atcgtcgaaa ccattgcggg
    19261 caaaaacacg atgggcatcg agttgcccaa cgacaaacgc caagacgtga tgttgagcga
    19321 aatcttgtcc tcgcccgtgt ttgccgaagc caaatccaag ctgaccgtcg cgctgggcaa
    19381 agacatcgcc ggtacgcccg tcgtcggcga cttggcgaaa atgccgcacc ttttggtcgc
    19441 cggcatgacc ggttcgggca aatccgtcgg cgtgaacggc atgattatgt ctatgctttt
    19501 caaagctacg cccgaagaag tccgcttcat tatgatagac ccgaaaatgc tcgagttgag
    19561 catttacgac ggcattccgc acctactctg tcccgtcgtt accgatatgc gcgaagcagg
    19621 gcaggcgttg aactggtgcg tcgccgaaat ggaaaaacgc taccgcctgc tctcccacgc
    19681 cggtgtacgc aatctggaag gcttcaacca aaaagtcgaa caagccaaag cggcaggcaa
    19741 gccgctgctc aatccgttca gcctgaacct cgacgaacct gagccactgg aaaaactgcc
    19801 gatgatcgtg gtcgttatcg acgaacttgc cgacctgatg atgaccgaac gcaaagctgt
    19861 cgagcagcaa atcgcccgcc tcgcccaaaa agcgcgcgcc gccggcatac acatgattgt
    19921 cgccacccaa cgcccaagcg tcgatgtcgt aaccggtctg attaaagcca acatcccgac
    19981 gcgtatggcg tttaccgtgc aaagcaaaat cgacagccgt accatcctcg accaaatggg
    20041 cgcggacgaa ctgctcaaat atggcgattc gctgttcctg caaccgggca gtgccgaacc
    20101 gacccgcctg caaggcacgt ttgtttcaga cggcgaagtg catcaagtcg tcaactacgt
    20161 caaatcgcaa gccccagccg actatattga aggtctgctc agcggcgaag ccgcgctgga
    20221 aactgccaat atcgttaatc cgaatgcaga cagcgacgaa ttattcgatc aggcagtcgc
    20281 ctatgttttg gaaagcaaaa aaacctccat ttcgtctttg cagcggcagt tgcgtatcgg
    20341 ctataaccgc gcggcaaacc tgatggaggc actggaaaat gcgggtatcg tttctccctc
    20401 cgacctcaac ggcagccgta aaattttggc gcacaaggac catttatagc ccgtatcgca
    20461 aaatgccgtc tgaacggcgg aattggcgtt tcagacggc
//
LOCUS       DAXDZG010000037        20258 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXDZG010000037 DAXDZG010000000
VERSION     DAXDZG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20258
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.037"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            40..686
                     /locus_tag="ACFB78_001156"
                     /pseudo
     CDS             40..686
                     /locus_tag="ACFB78_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(758..2503)
                     /gene="recD"
                     /locus_tag="ACFB78_001157"
     CDS             complement(758..2503)
                     /gene="recD"
                     /locus_tag="ACFB78_001157"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC1269152.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRSINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALDSMLERVFSQE"
     gene            complement(2570..3265)
                     /gene="lolD"
                     /locus_tag="ACFB78_001158"
     CDS             complement(2570..3265)
                     /gene="lolD"
                     /locus_tag="ACFB78_001158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC1269153.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3258..4505)
                     /locus_tag="ACFB78_001159"
     CDS             complement(3258..4505)
                     /locus_tag="ACFB78_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC1269154.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4731..5009
                     /locus_tag="ACFB78_001160"
     CDS             4731..5009
                     /locus_tag="ACFB78_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269155.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5065..5682)
                     /gene="recR"
                     /locus_tag="ACFB78_001161"
     CDS             complement(5065..5682)
                     /gene="recR"
                     /locus_tag="ACFB78_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC1269156.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5749..7287)
                     /locus_tag="ACFB78_001162"
     CDS             complement(5749..7287)
                     /locus_tag="ACFB78_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC1269157.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKNFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7336..7710)
                     /locus_tag="ACFB78_001163"
     CDS             complement(7336..7710)
                     /locus_tag="ACFB78_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC1269158.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7860..9488
                     /locus_tag="ACFB78_001164"
     CDS             7860..9488
                     /locus_tag="ACFB78_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC1269159.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLRQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9567..10799
                     /locus_tag="ACFB78_001165"
     CDS             9567..10799
                     /locus_tag="ACFB78_001165"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC1269160.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11218..11526
                     /locus_tag="ACFB78_001166"
     CDS             11218..11526
                     /locus_tag="ACFB78_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269161.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11608..12639
                     /gene="ruvB"
                     /locus_tag="ACFB78_001167"
     CDS             11608..12639
                     /gene="ruvB"
                     /locus_tag="ACFB78_001167"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC1269162.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMDEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12884..13567)
                     /gene="rpe"
                     /locus_tag="ACFB78_001168"
     CDS             complement(12884..13567)
                     /gene="rpe"
                     /locus_tag="ACFB78_001168"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC1269163.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13713..14148
                     /locus_tag="ACFB78_001169"
                     /pseudo
     CDS             13713..14148
                     /locus_tag="ACFB78_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
     gene            complement(14161..15024)
                     /locus_tag="ACFB78_001170"
     CDS             complement(14161..15024)
                     /locus_tag="ACFB78_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1269164.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLALLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15032..15649)
                     /locus_tag="ACFB78_001171"
     CDS             complement(15032..15649)
                     /locus_tag="ACFB78_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC1269165.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15812..16426)
                     /locus_tag="ACFB78_001172"
     CDS             complement(15812..16426)
                     /locus_tag="ACFB78_001172"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC1269166.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16633..18402
                     /locus_tag="ACFB78_001173"
     CDS             16633..18402
                     /locus_tag="ACFB78_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC1269167.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18399..19055
                     /locus_tag="ACFB78_001174"
     CDS             18399..19055
                     /locus_tag="ACFB78_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC1269168.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLAECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19256..20258)
                     /locus_tag="ACFB78_001175"
                     /pseudo
     CDS             complement(19256..20258)
                     /locus_tag="ACFB78_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4670 a         5847 c         5077 g         4664 t
ORIGIN      
        1 ttggatatgt ccaatcctac agtgttacgc aatatataag gggttgccgt tccgcagttg
       61 ggcggcagca tacacgttcc tttcggcgta ttctcgccgt tgcgcggcga atatctcgac
      121 ctgcttgccc atgcgccgtc aacgggtttt cagacggcat tcgatatcgg gacgggctcg
      181 ggcgtacttg ccgccatttt ggcgaaacag ggcattcctt ccgtcatcgg cacggatacc
      241 aatccgaggg cggttgcctg cgcccgtgcc aatattgccc gtttgggttt tgaaaaacag
      301 gttgaaatcc gcgaaaccga tttatttccc gaagggtttg ccgacctgat tgtctgcaat
      361 ccgccctggc ttcccgccaa gccgacttcc gccgtcgaat ccgcgttata cgaccccgaa
      421 tctgcgatgc tggctgcgtt tttgcgtgat gcgccaaaca tctgaatccc gacggagaaa
      481 tccgtcttat catttccgac ctcgccgtgc atttggggct gcggtctgcc gactttttgg
      541 aaaaggcatt tatccgggcg ggtttgcgtg ttgaggatgt tttgaaaacc aaaccggttc
      601 acaaaaaagc tgccgattcg aacgatccgc tggcttttgc ccgaaacagg gaaactactt
      661 tcctgtgccg tttgaaaaag gcataagggg cggtggcgtg cttccgacgg taggcatcgg
      721 aacgcccgcg ccgccggcac ggcatcattc gggcggatta ttcttgtgaa aatacccgct
      781 caagcatact gtccaacgcc gtctgacgga ctttgacggt gtttaccgcc tgacaaaatg
      841 tctttttgcc gccgaaaaat acgaattttt ccctggcgcg ggtaatggcg gtatataaca
      901 gctccttact caatccggac aatgcatcgt ccccttcgtc cgaaggtgcg tcggaaggcg
      961 gcagcagcca gacttcccga tattccgaac cttggctttt gtggacggtc atggcgaatg
     1021 cgggttcaaa ttcgggcagg cagcttaccg ctaccttttt aaatccgtcc gcatcggcaa
     1081 aataggcggc aaggctgccc tgccgtccga catcttccat aatcagtccg atgtcgccgt
     1141 tgaacagctc aagcgcgtag tcgttctgtc tgatcataat cggctctccg gcgaaatagg
     1201 ccaaatgttc cggtatgttc attttgcggc gtacatagct gcaataggct tcgttgaagt
     1261 cttccgcatc ctgccgccaa gctgccagaa ccacgatatc cgagatacct gcgtatgcgg
     1321 cttcgatatt gccgtctttt accgcctgcc aataggcttt gtgcgcccgg tacaaccttt
     1381 cgacccgagc attcggactg cattccgaat gttccagttc gtccggaaac cggtcaaaca
     1441 atgcccacgc cccttcatcg cccgatacgg cggcacgggc aaggcagccg atgccgctgt
     1501 tgtcgccgaa gcggtggctg aacgacagat gggcggtgtt ttgcgccaac acgggcggat
     1561 ttgcgctgac gctgaaaccg tgttccggaa ggaaaccggc cagcctttgg tgcgtttctc
     1621 cgtccaaaac ggttttttgc gacaaaacgg acagcaccgc cccgattccg acggacggga
     1681 gctggttttc atcgcccagc agaatcacgc gcgcgccggt tttgaccgct ttcaaaagtt
     1741 gcagcatcaa tgcggtatcc aacatggagg cttcatcgac aatcagcacg tcgaacggca
     1801 gcggacggat atggtcgaac gccgcctgca ttttgggcgg gctcagctta agcagtcggt
     1861 ggacggtttg cccttccagt ttgagcaaat ggcggcggac ggcctccggc gcgtcaaaac
     1921 cgttgattga acggtgcagt gcgcgcgcca tatgtgccgc cgctttgccc gtcggtgcgg
     1981 caagcgcgat atggggaaga ttttcgtttt caccgcaaat cagtgccagc agtttggcaa
     2041 ccgttgtcgt tttgcccgtt cccggccctc cggtaatcac cataaaaaac tgcaacagtg
     2101 ccaaggcggc cgcatcgcgc tgtccttcgc ttcccgcgcc ttgaaaccat tttgcgaggt
     2161 tttgccgcgc gcctgccgcg tcgggggcgg acgtgccggc tgccgccaag cgttttattt
     2221 cggcagccaa atcgtattcc aactgccaca tcctgcccaa aaacagcctt ctgccttcca
     2281 aaatcaaagg cgcggcggat gttccgacca cgggtgcgag tgccgacagc gcgtcagcct
     2341 cgtccccgct caaacggata aacgaatgac cgttttgcaa tgcctgaaac aggcgttcgg
     2401 tacagtttgc aagcacttcg ttgcccgaac ccgcatagcg ttccaaaaaa cggattgccg
     2461 cccttgccgc cgcttgggca aattcatctg tctgcagttc catcgtattc cttgtcgaaa
     2521 tgctgtctga aggcacgggg cttcagacgg tgcgtgttgt ttcggctgtt taggcgtttg
     2581 cgccctgtct ggggtgcagg ctgccgtctt tcatgaccat cacgcgctcg aagcggccgg
     2641 cgagttcgtc gtcgtgcgtt acgaccacca gccccgttcc caattccgtt ttcagttcca
     2701 gcatcatatc caaaacattc cttgcgtttg cccggtcgag gttaccggtc ggttcgtcgg
     2761 caagcaggca tttgggttgc gtaaccaagg cgcgcgcaat ggcggcacgc tggcgttcgc
     2821 cgccggacag ttcgcccgca cggtgcgtcg aacggtgttt cagtccgacc ttgtcgagca
     2881 tcgccattgc cgtttctacc gcctctttac ggcttttttt gccgatcaga agcggcatca
     2941 tcacgttttc cagtgccgaa aattcaggca gaagatgatg gaactggtac acgaaaccga
     3001 gatggcggtt gcgcaaatcg cccaaacgcc gctggtttaa ggcacgcaaa tcctcgccca
     3061 tcagcagcac cctgccttca gacggcatat ccagcccgcc caaaatatgc agcagcgtcg
     3121 atttgccgct gcccgaagag ccgatgatgc cggtgctttc ccccgtgcgg atttccaaat
     3181 ccaagccgta cagcaccgga acgtccaaac cgccgtcacg gtagcgtttg cccacgcctt
     3241 cgcatttcaa aatcaaatca ctcataacgc aaagcctccg ccggttgggt ttttgacgcg
     3301 cgccagctcg ggtacagtgt cgccacgaaa gacagcccca aggaaatgca ggcaatcagg
     3361 gcaacgtcgc ccatatcgac atcactgggc aggtagtcga taaaataaac ctgcgaattg
     3421 atgaggtgga caccgagcag gttttcaaaa aacgccacga ccctgccgac gttccaaccc
     3481 caaagcacgc cgcagaccac acccgccagc gtgccgaaaa agcctgaaaa cgcgccctgc
     3541 accataaaaa tcttcatcac gccggcaggg gaaagaccca aagtccgcaa aatcgcaatg
     3601 tccgcctgct tttccgtaac cgccatcacc aaggaagaga caaggttgaa cgccgccacg
     3661 gcgataatca gcgtcaggat aatgaacatc atccgttttt ccagttcgac cgcttcaaaa
     3721 tagctgcggt tgctgtacgt ccaatcgcgc acccaaaccg tgtccctttg cgcctccgga
     3781 atcagcgttg ccgtcaaggc gggggcgttt tgcggatcgg caagcttcag ccgcagcccc
     3841 gcaacttcct tatccaagcg gtacagtacg cgcgcgtctt ggatatgcgt catcgccaat
     3901 gagttgtcca cttcgtaaac acccgtcttg accaggccga ccacggtaaa ctgcttcagg
     3961 cgcggcacga ctccggcagg ggtaacgttg ccctccggcg taattaccgt aaccttgccg
     4021 ccgacttccg cccccaaagc ctccgccaaa ccgacgccga ggataatgtc aaactcgccc
     4081 ggaatcaggt cttcaaactt gcctgccggc attttgtcgc cgtattccac cactttgcgc
     4141 tcttcagacg gcaaaatgcc gcgtatctga acgcccctga tttcgcccgc gtttgccagc
     4201 aatgcctgat tggaaacata gggtgcggca gccaaaatgc ctttgcggtt ttcggcaaac
     4261 cgaagcaggt tgcgccaatc cgtatccgta ttgtcgatat agccgatttc ggcgtgcggc
     4321 gcgacattca ggagctgccc gcgtatttct ttctgaaagc cgttcataac cgacaagacg
     4381 acaatcagcg cggttacgcc caaggcgatt ccggcaatcg aaaccatcgt gataaacgac
     4441 ataaagccgt tgcgcttttt cgccctgaga tacctcaagc ctatccaagc ctctagagaa
     4501 aacataacgc taccttaaaa atgtctgcaa acgtgccgcc ccggacggcg gtttggggga
     4561 ggcggcaaaa gtttattgta ccgtaaaacc ccggcagcgt ccgaacggca ggctgtgtaa
     4621 ataaagacgc ggtatgtggc aaattcggaa aaacaggacg gaaaacttgc acaacgcgcc
     4681 gaactaccct atccttctct tgaaacaaaa ccttttcttt aaggaaaaca atgaatatca
     4741 ggaaaatctc cgctttgtgt gctgttgccg tttttactgt ttcgacagcc tatgccaaag
     4801 aaatcaaaat cgatgccaac aacacgcctt attccgaagc cgacgcgcaa aagctggcgg
     4861 caacggcagt cggtatgggc gttaaggaac ctgtcagcct gaacggcggc agcggcagca
     4921 ttaccgtttc cggcagcagc gcggcgcagt gcgtctttac ggtcggcagc gggggcactt
     4981 tgcagattca agggctcaac tgcaagtaaa ccgcccgaaa aaatgccgtc tgaaggcttc
     5041 agacggcatt ttgcattggc ggcgttacgc cccgccttct ttaatcagac gccgttcgta
     5101 caccgcctgc gccagcgttc ccgcatcgac atattccaat tcgccgccca atgggatgcc
     5161 ctgcgacagc ctgctgacct tgtaaggcag gtttttgaaa aactcggaca ggacatacgc
     5221 tgtggcattg ccttctgcgg taaaggcggt cgcgataatg atttcttcga tttccccgcc
     5281 gtccagccgt tgcgccagcc tgtccaatgc gatggcggat acgtccattc ccaatgccgt
     5341 attgatttgc cccatcagga cgaaatacag cccgtcgtgg cagtttgccg cttctatatt
     5401 cgacacgtcg gcaggcatat gcaccaccat cagccgccgc ccgtcgcgtg tttcatcggc
     5461 acaaatatcg cacaatccgc cttcgcaaaa tgtattgcac cttgcgcaat ggcgaacctg
     5521 cctcaatgcc gtctgtaagg catccaccag ctcttcagcc tctttgcgct tttgctgcaa
     5581 cagatggtac gccatccgct gtgccgattt cggcccgacg ttgggcaata ctttcagcgc
     5641 accgatcagg cgttggaatg catcctgttt tttggaattc atcatactcc gccatatggg
     5701 aaaacggtcg gaatattccg accgttatgt tgtcaacaaa agtgtcaatt actgaccatc
     5761 gccgttgtca accgattgtg ctcctttagt ctgtttgatt tttccgttga aatagcggat
     5821 cagcaggtcg aaagtattgg cagactgctg ttgcgccaaa gcctgtttcg caggaggaag
     5881 ctgtgcggca atatcctccg gaggcgtgac tgcctgcacc tcgacaatca cgggtgccgg
     5941 cagaccggtc agtctgacat aggcgggttt gccgtttgcc ggttttgctt tcagcagttc
     6001 cgcataagcc tcgggcggca tggactgcct tgcctgctgc gcgcccaaaa cggacacttc
     6061 cgaccatttc acgtcaactg ccttgccgcc gttcagttgg gtaagcactt cttttgcctt
     6121 gttttcggca agtttggcgg cttcggtacg gatataggcc tgacgcaccg catctttggc
     6181 ttcttcaaac agtaggtttt tttcttcgcg gacttctttg gcgcggacga cccacgcggt
     6241 ttcgctgttg atggtcagca cttcggaatt gtgttttttc ttcaatacgt cgtcgctgaa
     6301 tacggcattg attaggtttt cgggcatgcc ggacatttgt gcgtcctgcc tgctcagcca
     6361 agtttcttgg gtttccactt tcaaaccgct gtttttggcg gcttcggcaa gcgaggaggg
     6421 atgattgaac gcatcgtcgc ccagcttttc ttttgccttg ttgaagtcgg caaccgcctt
     6481 tttcattttc aattcgtttt cgacggcggc tttttcctgc tcgaaagaag gtttggcttc
     6541 atgtgccggc aaacgcgcca cgcgctcttc aaacgcattt ttcacttccg tttcactgac
     6601 ggtctgcttg tctgcaaaat tcttcagatt caaggcgaca tattccaatt tgaccgcctg
     6661 cggcagcaga tagtcttttt tgttcgcatt ataaaatttc tgcaaatcgg cttcagacgc
     6721 tttgacttgg gcgatgaact cgtcggggtt gaaagtgtgc gaacggatgg tgcggttgac
     6781 ctgcgtcagc ctgatcagct gttccgcctg cgcgtcgccg accaatacgc cgttttggac
     6841 gaggcttacc aaattctgca aggcaaactg atcgcggatt tcttcgacaa actggtcttc
     6901 agacatatgg cgttgcgaca ggtattgact caaaagcgcg tgactgaatt tgccgtttgc
     6961 gtcgtggaaa ttgggatcgt ccacaatcat ctgcttgatt tgttcggaag aaaccgaaat
     7021 gcccatcagc ttcgcgccct gtttcaggta ggcgcgttgc agcagggatt ggaacaccgc
     7081 gtcgcgccaa gggctgccgc cgtccgcctg ctcgttctgc atggcgttgt tgattgagtg
     7141 ctcgctgatt ttttcgtcgc ccacttggac gatgtagtcg gcgcccggat gggaaaccgt
     7201 gctgacgccg aagccgacaa aagttaatgc aatcaggcct aataagactt gggcgggtgt
     7261 tctgtatttt tcgatggaat ggaacatatt ttcagattgg gaatgggaaa ttcaagtcgg
     7321 gtattgtaac ggtttttatc cctgtctgca cggggcttgc cggttgaaga tgccgtcgta
     7381 ggtttcttcg ctgaagccga cgtaaaccct gctgccgcac tccaatacgg gacgcttgat
     7441 caggctcggc atttcggaca tcagtttgac ggcctccgcc gtcgaggaca gggctttttg
     7501 ctgtgtttcg gcatcgagtt tgcgccagct tgtcccgcgt ttgttgaaca gggttgccaa
     7561 aggcacttgt tccagccacg agcagatttc cgcttcagac ggcatctgtt ttttgaaatc
     7621 ccgaaaccca aactccaagc cgtatccggc aagcctgttt ttggcttttt tgactgtgtc
     7681 gcaatttgga atgccgtgaa ggattatcat ttggaaacct tttgcctgaa ataataaaac
     7741 cgatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacggc ggggcggtta
     7801 tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggtt ttacccataa
     7861 tgatttctac caacggcatt accatgcagt tcggcgcgaa gccgctgttt gaaaacgtat
     7921 ccgtaaaatt cggcgaaggc aaccgctacg gcttgatcgg cgcgaacggt tcgggcaaat
     7981 ccaccttcat gaaaatcctc ggcggcgatt tggaacagac cgccggcgaa gtggcgattg
     8041 aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa gacatgcgcg
     8101 tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc gagcgcgatg
     8161 cgatttacgc caatcccgaa gccaccgaag acgactacat gaaagccgcc gaactggaag
     8221 ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg cgccgccgaa ttattaagcg
     8281 gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc ccgggcttca
     8341 aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg ctcttggacg
     8401 aaccgaccaa caacttggac atcaatacca tccgctggct ggaaggcgtg ttgaaccaat
     8461 acgactccac catgatcatc atctcgcacg accgccactt cttgaacgaa gtttgcaccc
     8521 acatggcgga tttggactac aacaccatca ctatttatcc gggcaactac gacgactata
     8581 tgctcgcttc cgcccaatcg cgtgagcgcg ccctgaaaga caatgccaaa gccaaagaga
     8641 aactgcaaga gctgcaagaa ttcgttgccc gtttttccgc caataaatcc aaagcccgtc
     8701 aggcaaccag ccgtctgaga caggccgaca aaatcaaatc ggaaatggtc gaagtcaaac
     8761 cttctacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc aagctgcacc
     8821 gtcaggctgt ggaagttgaa aaactggcca aacgctttga aacccagttg tttagaaacc
     8881 tcaacttcat ccttgaagca ggccaacgcc tcgccatcat cggcccgaac ggcgcaggta
     8941 aatctacctt gctgaaactc ttggccggcg cgtacaaccc cgaatattca gacggcctgt
     9001 tgccggacga aggcagcatc aaatgggcgg aaaaagccag tgtcggctac tatccgcaag
     9061 accatgaaaa cgactttgac gtcgatatgg acttgagcga atggatgcgc caatggggtc
     9121 gggaaggcga cgacgaacaa gtcatccgag gcactttggg gcgtttgctt ttcggcagca
     9181 acgacgtggt aaaaaaagtg aaagttctct ccggcggcga aaaaggccgt atgctttacg
     9241 gcaaactgtt gctgttgaaa cccaatgtct tggtcatgga cgaaccgacc aaccatatgg
     9301 acatggaaag catcgaatcg ctgaacatgg cactggacaa atacaacggc acgctgattt
     9361 tcgtctccca cgaccgtcag ttcgtttcct ccctggcaac ccaaatcatc gaactggacg
     9421 gcaaaggcgg atatgaacac tacttgggcg attacgaaag ctatctcgag aaaaaaggcg
     9481 tagcataagc gcacccctcc ggttgaaaca atgccgtctg aagccgcttc agacggcatt
     9541 gttgataact ttaaaatagg aagcatatgc agacttacct cgtcggcggc gccgtccgcg
     9601 attatctttt gggcctgccc gtcaaagacc gcgattgggt ggtcgtcggc gcagacgcgc
     9661 aaaccatgtt ggcgcaaggt ttccagccgg tcggcaaaga ttttcctgta ttcctccatc
     9721 ccaaaacgca cgaagaatac gccctagccc gcaccgaacg caaaaccgcc aagggttacg
     9781 ccggtttcag tttccacgcc gacaaagacg ttacgctgga acaggacttg atgcgccgcg
     9841 acctgaccat caacgcgatg gcgcaagatg cggacggcaa gattatcgac cctttcggcg
     9901 ggcaacggga tttggcggca ggcattttgc gccacgtctc acccgccttc gccgaagatc
     9961 ccgtccgcat ccttcgtgcc gcccgctttg ccgcgcgtta cggctttgaa atcgccgaag
    10021 aaaccataaa gctgatgcgg cagatggtgg aaaacggcga agcggacgca ttggttgccg
    10081 aacgcgtctg gcaggagttg gcgaaaggtt tgatggaaaa aaatccgcgc aaaatgattg
    10141 aaatgttgcg cgaatgcggc gcactccaag tcttgctgcc cgaagtcgac gccctcttcg
    10201 gcgtgccgca acgcgccgac tatcatcccg aaatcgacag cggcatccat accctgatga
    10261 cgctgcaacg cgccgccgat atgggtttga gcctgcccga acgctatgcc gccctgctgc
    10321 acgacttggg caaagccaaa acaccgcccg acatcctgcc gcgccaccac ggacacgaca
    10381 tcaacggcgt agaacccgtg cgcgaagtca atcaacggct gcgcgcgccg aggcagtgtg
    10441 ccgagcttgc cgaattggtt tgccgttggc acattatttt ccaccaagtc ggacagctta
    10501 aaagccaaac cattctgaac gtattgaaaa aaaccgatgc cttccgccgt cccgagcgtt
    10561 ttcagacggc attgaacgtc tgcattgccg acacgcaagg ccgtctgaac cgagaacaca
    10621 cgccctaccc ccagcgcgcg cactggctcg ccctgctcga agccgccaat caggcggatt
    10681 cgggcaaaat cgccgccgaa tgccgcgcgc agggaaaagc gcactttatc gccgaacaaa
    10741 tcgaccgggc gcggctggcg caaatcgccc cgctgcaaaa agcgtttcgg ggcgcgtagg
    10801 aaaaagaaaa cgccaaacgc ggcatctttt gaggttcgcc cgcattgccg aaacagcgtc
    10861 ggagaaaggg atggatgaca tcggtttatt cggctgcctt ttaatattcc cttgatgcag
    10921 gcctgattag tcggacaaaa ataccttttg ttcaacggaa ccgatttcta tccggacgga
    10981 aacagccggc tttccccttg caaacggatg gaatcaagcg ggtattcaaa cacagccgtt
    11041 tatttttatt ccgccgccaa gttatgggat agggtttgcg tgtttcgggc aggcaaaccg
    11101 cgcaacctta gacgcagtcc gaagttttga aggcggcatc cgggccgggt tttgaaacag
    11161 ccgccgccat atccgctata atccatcctt cagccattcc gcccccgaca taaaatcatg
    11221 accctgaaaa ccgatttatt gcctaaaatc aacaacgaag attatcaacg cctcatcctc
    11281 aaacatagcg cggaattcag cggcggagaa acccgcctgt tgaacgaaat cctcgaaaaa
    11341 ttcaatttcg acgttgttca ggcgcaggca ttggcgcagg cggtaatgca gcaagttcgc
    11401 ttcgatccca acgcctacca catcgacagc gacgacgaag acaccaccgg catctgcccc
    11461 cactgcatca acccgcctat gccgcccctg cgcgactatc tcgtttggcg cgaaacacgc
    11521 ggataaaacg cttttgaccg ttatcttttc aatgccgtct gaaacgccgc cgaccgttcg
    11581 gacggcatac ccgacaaagg gaacactatg ctgcaaaccg acaacctgac cgccgcgcaa
    11641 ccgcaacgca tcgttgccgc ccaaaccgcc tccgcacagg aagaactgct cgaacgcgcc
    11701 ctccgcccca aaacgctgga cgactacatc gggcagcata aagccaaaga gcagctcgcc
    11761 atcttcatcc aagccgccaa aaaacgcggc gaagcactcg accacgtcct gcttttcggc
    11821 ccgcccggac tgggcaaaac cacccttgcc cacatcatcg ccaaagagtt gggcgtaaat
    11881 ttgcgccaaa ccagcggccc cgtcctcgaa cgcgccggcg acctcgccgc ccttttgacc
    11941 aaccttgatc cgcacgacgt attgtttatc gacgaaatcc accggctaag ccccgtcgtc
    12001 gaggaaatcc tctatcccgc gctcgaagac taccggctcg acattatgat aggcgaagga
    12061 cccgccgcac gttccgtcaa aatcgacctg ccgcccttca ccctcgtcgg cgcgaccacc
    12121 cgcgccggta tgctgaccaa cccgttgcgc gaccgcttcg gcatcgtctc ccgcctcgag
    12181 ttttacgaaa accgcgacct taccaccatc gtcagccgtt cggcacaact gttgcagctc
    12241 gatatggacg aagaaggcgc gatggaagtc gccaaacgca gccgcggcac gccccgcatc
    12301 gccaaccgcc tgttgcgccg cgtgcgcgat ttcgccgacg tgaaaaacaa cggcgtaatc
    12361 gatgccgccg tcgccgatgc cgctttaagt atgctggacg tggacgcgca ggggctggac
    12421 gtgatggaca ggaaattcct cgaagccgtt ttgcacaaat tcggcggcgg ccccgtcggt
    12481 ttggacaacg ttgccgccgc catcggcgaa tctacggaca ccatcgaaga cgttatcgaa
    12541 ccctacctca tccaacaagg ctttttgcaa cgcaccccgc gcggcaggat ggcgaccgaa
    12601 cgcgcctacc tgcatttcgg gctgcccgtc gaaaaataaa ccatgccgtc tgaaaccgaa
    12661 ccgggtttca gacggcatgg ttatcagacg tttgcaccgg caccggtttc atcacttaac
    12721 agaaaccttt tgtccggcag gacagattct ccccgatgaa aataactgtt tccctggcaa
    12781 acggttgaaa cccgtctggc tgccggttga aaaataacgc aatgctgtct gaaacagagc
    12841 taattttcag acggcatttc tatttcaatc attggctcaa ggttcagcct gccgcttttt
    12901 ccagttccgc cctcatcgca gcgattaccg ccttgtaatc cggtttgccg aaaatcgccg
    12961 aaccggcaac aaaggtatcc gcaccggctc gggcaacggc ggcaatattg tcagttttga
    13021 tgccgccgtc cacttcaatg gcaatacgtc tgccgctttt cccttcatag cggtcaagca
    13081 tggcgcgaac ctgtctgatt ttttccaaag tgtgcggaat gaagctctga ccgccgaacc
    13141 cggggttgac cgacatcagc aaaaccatat ccagcctgtc caacacgttt tccaacacat
    13201 atacgggcgt tgccggattc aacaccagcc ccgcctgaca gcccatgtcc cgaatcaggc
    13261 tcaagctgcg gtcgatatgg cggctcgctt cgggatggaa agtgataatc gatgctcctg
    13321 ctttggcaaa cgattgaatt aaatcgtcaa cgggttcgac catcagatgc acatcgatcg
    13381 gcacgcttgc ataaggcttc aacgccgcgc agaccatagg tccgaaggtc aggttcggca
    13441 cataatggtt gtccatcacg tcaaaatgga tcagatccgc acccgccgca atgacatttg
    13501 ccacctcttc tccgaggcgg gcaaagtctg ccgacaaaat actgggtgcg atacggtagg
    13561 cggtcatatt ttttccttca atgcctttat gggcggactc aacgcgggaa agcctcgaaa
    13621 cagcattatt aacaaaagtt aactgccata ataccatctt tcacacggca atcaagtata
    13681 ttgtccgaaa tatccggatt aaaggaaaag ccgtgccgct gcctgctccc tgccgttttg
    13741 ccaaacctgc cgcctcttta tttaagtatg gctttgcttt cctgccagct ttcccacgcc
    13801 gccacggctt atatcccccc gaacgatttt caaccgaact gcgacatacg ccggctcggg
    13861 ctgacacagg gtcagcacaa tgagctgcgt aaaatccgcg ccgccttcaa aatggcgggc
    13921 gacagggcgc gtttgaaggt tatgcattcc gaacacagcc gccgccgctc tgtcgtcgaa
    13981 atcatttctt cggatgtttt taatcggaac gaggcgcgcg attatgtcga aagccgctac
    14041 cactccagca tggattttgc ggtggacgaa ttggaaatcc aacaccgctt cttccatatt
    14101 ctcacaccgc aacagcagca aatgtggctt tcttcctgcc tcaaataatc cccgaaacac
    14161 tcacggcgca cgttgctccg gcagcctgcc cgcccagtcg caggcaaact gccaagcgga
    14221 acgtcccgag cggttgcccc gcatctgcgc ccactgcaac gccgccgtcc gggcggcttc
    14281 gtcatatggc acatcaaaat cttccaacca gcttcgcact gccgccagat aatcgttttg
    14341 atcgaacgga taaaaactga gccacaatcc gaatcggtcg gacaaagata ctttttcttc
    14401 cacggcctct ttttgatgga tttctccgcg cacgcctgtc gtacctgcat tttcatccaa
    14461 atattcgggc atcagatgcc gtctgttgga agtcgcgtac accatcacgt tggcgcaacg
    14521 ttgggacaga ccgccatcca atgccgtttt caatgcctta taggtttcgt cgccgctttc
    14581 aaatgacaaa tcgtcgcaaa acacaatgaa tttttccggg cgttccttca aaagtgccaa
    14641 cagatacggc aggccgatca aatcgctttt atcgacttcg atcaggcgca atcccttatc
    14701 cgcatattcg tgcagcaggg ctttgaccag cgaggatttg cctgttccgc gcgcgccgct
    14761 catcaatacg ttattcgcag gtctgccggc aataaattgt tcggtattac gcaccagcaa
    14821 ttcggtctgc ctgccgactc cggcaagccg cgacaaaggg aaaacgtgcg gatcgggcaa
    14881 gtgttccaaa aaaccttttt tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc
    14941 cgtatgcccg ggttcgggcg gaagcacggc atccaaacgc cgcaaaacgg cataggcttt
    15001 atcgaggaat tcgttcaatt ccatctctac ctcactttgc atatctttgc gccatcagcc
    15061 gttcgacggt gtcgacgatt gcctgcgtat tcggatcgat ttcgatgttg atcctgtctc
    15121 cggcctttct gctgccgaac agcgtccgtt ccaaagtttc gggaatcaga tggacattga
    15181 aacggctgtc ttcgactttg cctatggtca ggctgcaacc gtccaagccg acgaaccctt
    15241 tggtcaggat atagggtttg agttcgtgcg ggagcgcaaa ccaaaccgtg cggttgaacc
    15301 cgtcccgttc gatttcgaca atgggcacgg ttgccataat gtgtccgctc atgacgtgtc
    15361 cgccgatttc gtcgccgaaa cgtgccgccc gttcgatgtt gacgcaatcg ccttccttca
    15421 gcagccccaa attggttttt gccaaagttt ctgccattaa atcgaagctg acgcggtttc
    15481 cttcgatttc agtaatcgtc aggcagcagc cgttattggc gaccgatgcg ccgtgttgca
    15541 ggttttctgc cgcctcttgc ggaagctcga cgacataagt ctgaaacgcc tccgacgggc
    15601 ggtggattgc cgtcagtttt cccaatcctt gaacaatgcc tgtaaacata atcctgtttc
    15661 cctgtgtcgg taaaaatggt gcaaattgta gcatttcccc gcgaaaaatg ccgtctgaaa
    15721 ctccttcaga cggcattgtg cctccggttc gggcaaaaac cgcccggtac gccttgacct
    15781 ttcctttccg cgccggtcgg cggtcttgca cttatccctc ctgcaaatcg gtttgcgtgt
    15841 tcaagtcggc aaaatgcccg tcaaactcga atctgaccgg ccgcgccctt tgctgctgca
    15901 accagcttct taatgttttc atacccgaaa acagataagg aatcgcgctt tgcagaattt
    15961 gcggacggat atacataatg ttgtagtgca tcgttaccgg cgtttccaca taaaacgcat
    16021 tgcacaacgg tgtgcgtttg gagacgcttt caaagcgcgc gaccaaatcg tccggcagat
    16081 acggcatatc gcaaggtaca atcagcagcc aatcggccgc cgccaactgc aaatcgttgg
    16141 ctgcggtaca caatgccgaa agcgggccga aatgctgcca ctgccgggca tccggaaaaa
    16201 catgcgggct gcggcgggcg tattcttcca aattccggtt ggcactgatg acgatatggc
    16261 tgacctgcgg cctgatttta ccgataacgc ggtcaatcag cggtctgcct tccaacagtg
    16321 ccagcccttt gtcttccccg cccatacggt cggccaggcc tcctgccaaa atcaatgcga
    16381 acgtttttaa tgcgggcggg cttttgggaa agtttgaggt tttcatagtt tcagtctgcc
    16441 gtttccgata ttttagaagc gcgtttgaaa acggcgtcgt ataaattcat gttatttgaa
    16501 ttttatcata acgtgattta acactgaaag cattgaggct taagttttcc ttaacctgtc
    16561 ctttctgagc agttgtttct aattccaaag aatgatattg tttgcaccgt ttaaaacgac
    16621 tttcctccga atatgatact gccaacccga ttttcagacg gcatccccct ttccctgcgc
    16681 ctgaaactcc tgaccggctt gtgggtcggt ttggcggcac tgtctgtcgt tttaacgctg
    16741 ctgctttctt tccgcctgga aaatgccgcc tcggtcatcg aagaggcggg caatctgaag
    16801 atgcaggcat accggcttgc ctatatggcg ggcgaaggct cgccccgcgc acaaatcgac
    16861 aatcagattg ccgaatttga aaaaagttta aaacgcattt cccaaagcga tgccatccat
    16921 ccgctgattc cctccgacaa tccccttgcc tacgatttga tacagtccat gctgattatc
    16981 gactggcagg ccaacatcct ccccccgttg caggcatacc gccgccccac acaaatcgag
    17041 ctttaccgct ttgccggcaa tatcgaactg tttttgcagg cgttggaaaa tgccggcgaa
    17101 aaaaacactt ggtggctcag gcggtttcaa tgggtcatta tgctgatgac gctggtgtcg
    17161 tccgtgctga tgcttttttg gcaccagatt tgggttatcc gtccgctgca ggcgttaagg
    17221 gaaggtgcgg aacgcatcgg tcagcggcat ttcgacatcc ctgttcccga agacggtacg
    17281 cccgaattca aacaggtcgg gcggtgtttc aaccaaatgg cgctcaggct gaagacttta
    17341 tacgatgatt tggaaggaca ggttgccgaa caaacccaca accttgaaaa gcaaaaccgc
    17401 aacctgaccc tgctgtaccg gaccacgcgc gacctgcatc aatcctatac gccgcgacag
    17461 gcggcggaag aatttttgaa ccacatcctg cccgccgtcg gcgcgcaatc cggcaacatc
    17521 tgcctggaaa acggttccga cacagacatt tccgtccata cggccgaaca cggcaaaaag
    17581 ccccctttgg aaaaatacca tgacgaaacc ttccccatcg aatatcaaaa cgagaaactg
    17641 ggcatgcttt cgctcggctt ttcagacggc acttccctca ccggagacga ccgcacgctg
    17701 cttcaaaccc tgattcggca attgggcgta tcgctcgccg gggcgaaaca ggaggaagaa
    17761 aaacgtctgc ttgccgtatt gcaggaacgc agcctgattg cacaggggct gcacgacagc
    17821 atcgcgcagg cattaacctt cctgaacctg caggtacaga tgctggaaac cgcctttgcc
    17881 gaaaacaaac gggaggaagc cgcagaaaac atcggcttta tcaaaacagg cgtgcaggaa
    17941 tgttatgaag atgtccgcga actgctgctc aatttccgta ccaaaatcag caataaagaa
    18001 tttcccgaag ctgttgccga cctgttcgcg cgcttcacgc aacaaaccgg gataacggtc
    18061 gaaaccgttt gggaaaacgg ttcgttcctg cctacacagg acgagcagct ccaaatgatt
    18121 ttcatcctgc aagaaagcct gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa
    18181 ttcacccttt ccgaatacgg cggacgcttt accatgacca tccaagacaa cggacaaggt
    18241 ttcgacacgg agaaaatagg agaacctacg ggcagccatg tcggactgca catcatgcag
    18301 gagcgtgcca aacgcatccg tgccgtttta gaaatccgtt cccaagcaca acagggaacc
    18361 accgtctcat tgacgggcgc acccaaagaa agcctaccat gaccatcaaa atcatcctga
    18421 tagacgacca caccctcttc cgcagcggca tcaaagccct tttgtcgcgc caacacggtt
    18481 ttgaagtcat cggcgaagcc gcagacggac tctcgggcat caaaatgatc agtcggctgc
    18541 aacccgatgt cgtcctgctc gatttggata tgccaggcat gaacgggcgc gaggccttgt
    18601 cccaaatcat cagcatcaat ccgcagcagg cagtcattat gctgaccgtt tccgaagaca
    18661 gcgacgacct tgccgaatgt atgcgcatcg gcgcgcgcgg ctacctgctg aaaaacatca
    18721 acgccgattt tcttctcgaa agcatacgca aagccgccga aggcgataat gtattctcgc
    18781 ccgagatgac cgccaaactc gtcaaaagcc tgatttcccc ccaacctgcc caaaggacgc
    18841 aggcactctc ctcacttacc cctcgtgaac tggaaatctt gggctatctc gccgcaggac
    18901 acagcaacaa aatcatcgcc cgccacctcg atcttgccga atccaccgtc aaagtccacg
    18961 ttcaaaacct gctccgcaaa ctcaacctca gcagccgggt gcaggccgcc gtttacgcca
    19021 tccggcacaa cgtcccccaa cctgtgccgg aatagacgtt cagacggcat cgggtcagga
    19081 aaaaagcaat gcgccccttt gacggggtgc aatatataag ggacggcatc cggtgtgccg
    19141 tccctttaag tacgccatta ccgccgtaac ccggacggaa tggcttaggc agtttatttc
    19201 caagaatatt tcacacccac ggtgccttgg tagcctttat gtctgttgct tccgcatcct
    19261 gccgataatt gccttgcctg tcccgagaaa gggtttgatt ttcgccacgg cgttggtgga
    19321 gcgtatccag catcgcatag cctgattcca agttttgacg tggaacgagt gcgtaggccg
    19381 gtgcgcttgg cgtgatgatg gttttatctt gattcggcgt ggttaaagtc cagaagtatt
    19441 ctgtcgcgcc cgcctcatct ttgtgtgaaa ccaattgcaa ttcccccgcc cctgtggttt
    19501 tggcagttcc gctaaaagta tcgcgcattg tgtaggtatg tttagcgtct tcgatattta
    19561 atttaccggt ttccgccccc ttgtcttgtc ccaaaacctt aacaacggcg gcactgcgtt
    19621 tgtagcggtc ggaaagttcg ccgatcgagc cgtcgataat attttctttg ccatctttac
    19681 ccactgaaat gaccgttgtt tttcctgaag catcgcctgt gatttccagt aaatcagatt
    19741 gagaatttcc tccttgatca tcacccggcg agttccattc tgtattgact tttaacacac
    19801 cgtctttacc gacataattt ccttcaatgg tgagtttgtc tgcataggat tgattggcaa
    19861 gcgtaatgat gccgccgttg gtgaatgttc ctttttcaaa tacagaagtt gtacccgata
    19921 aagaaatacc tgtattagat agggttagcg tgccgcctga taataatgta gtcccttcgg
    19981 gtgttgttaa tgatgtgtct ttttttaggt gaaaagtagc ttttgttagt tttatggttc
    20041 caatatcttc tgatttgaat tccattgcgc catttgtaat ttttagcaca gcattggtaa
    20101 tagggagggg gaagcgcatt gtaaatgatt tttctgttcc atctggattg attaatgtac
    20161 cagatgcgcc aaagatctct ccttcaggga tagaaacagt tccatcagaa attgatggtt
    20221 tatttgcgct tttatcactg tgtttatgaa ttaatgta
//
LOCUS       DAXDZG010000038        19862 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXDZG010000038 DAXDZG010000000
VERSION     DAXDZG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.038"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..390
                     /locus_tag="ACFB78_001176"
     CDS             <1..390
                     /locus_tag="ACFB78_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1269169.1"
                     /translation="WHTYFVKGNQAETEGVWVHNSCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            387..806
                     /locus_tag="ACFB78_001177"
     CDS             387..806
                     /locus_tag="ACFB78_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC1269170.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            1054..1326
                     /locus_tag="ACFB78_001178"
     CDS             1054..1326
                     /locus_tag="ACFB78_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269171.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            1308..1460
                     /locus_tag="ACFB78_001179"
     CDS             1308..1460
                     /locus_tag="ACFB78_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269172.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            1447..1992
                     /locus_tag="ACFB78_001180"
     CDS             1447..1992
                     /locus_tag="ACFB78_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC1269173.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
     gene            2396..2797
                     /locus_tag="ACFB78_001181"
     CDS             2396..2797
                     /locus_tag="ACFB78_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269174.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            3109..4242
                     /locus_tag="ACFB78_001182"
     CDS             3109..4242
                     /locus_tag="ACFB78_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269175.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAQWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            4249..4569
                     /locus_tag="ACFB78_001183"
     CDS             4249..4569
                     /locus_tag="ACFB78_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269176.1"
                     /translation="MPSETGFQTALESQTLFAMPYMPNPVRYKLKTTIQKTEIHIIKQ
                     TNKLTYCAILRAVPYFPVLYMSGRHPHKRRDTDKEKSLGIKNILINQYKSNKQFIIFF
                     SVGV"
     gene            complement(4745..5020)
                     /locus_tag="ACFB78_001184"
     CDS             complement(4745..5020)
                     /locus_tag="ACFB78_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC1269177.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMLSET"
     gene            complement(5017..6300)
                     /locus_tag="ACFB78_001185"
     CDS             complement(5017..6300)
                     /locus_tag="ACFB78_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1269178.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFMRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(6449..7867)
                     /gene="glnA"
                     /locus_tag="ACFB78_001186"
     CDS             complement(6449..7867)
                     /gene="glnA"
                     /locus_tag="ACFB78_001186"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC1269179.1"
                     /translation="MSIKNAVKLIEKSKARFVDLRFTDTKGKQHHFTVPARIVLEGPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            8178..8987
                     /gene="aroE"
                     /locus_tag="ACFB78_001187"
     CDS             8178..8987
                     /gene="aroE"
                     /locus_tag="ACFB78_001187"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC1269180.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            8990..9691
                     /gene="mtgA"
                     /locus_tag="ACFB78_001188"
     CDS             8990..9691
                     /gene="mtgA"
                     /locus_tag="ACFB78_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC1269181.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(9965..10040)
                     /locus_tag="ACFB78_001189"
     tRNA            complement(9965..10040)
                     /locus_tag="ACFB78_001189"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10005..10007),aa:Phe,seq:gaa)
     gene            complement(10231..10965)
                     /gene="lptB"
                     /locus_tag="ACFB78_001190"
     CDS             complement(10231..10965)
                     /gene="lptB"
                     /locus_tag="ACFB78_001190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC1269182.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(11036..11566)
                     /gene="lptA"
                     /locus_tag="ACFB78_001191"
     CDS             complement(11036..11566)
                     /gene="lptA"
                     /locus_tag="ACFB78_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC1269183.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(11547..12128)
                     /gene="lptC"
                     /locus_tag="ACFB78_001192"
     CDS             complement(11547..12128)
                     /gene="lptC"
                     /locus_tag="ACFB78_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC1269184.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(12125..12661)
                     /locus_tag="ACFB78_001193"
     CDS             complement(12125..12661)
                     /locus_tag="ACFB78_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC1269185.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(12727..13701)
                     /locus_tag="ACFB78_001194"
     CDS             complement(12727..13701)
                     /locus_tag="ACFB78_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC1269186.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            13798..14853
                     /gene="tal"
                     /locus_tag="ACFB78_001195"
     CDS             13798..14853
                     /gene="tal"
                     /locus_tag="ACFB78_001195"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC1269187.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            14865..14999
                     /locus_tag="ACFB78_001196"
     CDS             14865..14999
                     /locus_tag="ACFB78_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269188.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            15004..15294
                     /locus_tag="ACFB78_001197"
     CDS             15004..15294
                     /locus_tag="ACFB78_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269189.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            15311..16198
                     /gene="gluQRS"
                     /locus_tag="ACFB78_001198"
     CDS             15311..16198
                     /gene="gluQRS"
                     /locus_tag="ACFB78_001198"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC1269190.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(16268..17284)
                     /gene="dusA"
                     /locus_tag="ACFB78_001199"
     CDS             complement(16268..17284)
                     /gene="dusA"
                     /locus_tag="ACFB78_001199"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC1269191.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            17404..18558
                     /locus_tag="ACFB78_001200"
     CDS             17404..18558
                     /locus_tag="ACFB78_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1269192.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKTGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLGIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAIMQAQSDIKSGK"
     gene            complement(18608..18874)
                     /locus_tag="ACFB78_001201"
     CDS             complement(18608..18874)
                     /locus_tag="ACFB78_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC1269193.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(18982..>19862)
                     /locus_tag="ACFB78_001202"
     CDS             complement(18982..>19862)
                     /locus_tag="ACFB78_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC1269194.1"
                     /translation="LRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
BASE COUNT         4844 a         5042 c         5187 g         4789 t
ORIGIN      
        1 tggcatactt acttcgtcaa gggcaatcag gcggaaacgg aaggggtttg ggttcataat
       61 tcttgtccgt atggcggttc aaataattta gagaaagcaa aacttcgcgc agaacggctt
      121 agtaaaaacg atcgggcagg aaaagacttt actaaagcag gaaaagaagc cgttattgat
      181 ttgaatagga ttcaaaataa tggtcaagtt aagtgcgcta attgcggtat tgaaacaatt
      241 ccagccaaac aatctataaa aaatatttct cctacttcaa atgaaagaca agtagatcat
      301 gtcatcccta aatctaaggg tggtcaaggt acacctaaaa acgggcaggt attatgcaga
      361 ggctgcaaca ttaagaagag taacaaatga aatcgaaact tactgtcgtc tattacgatt
      421 tggaaagcaa tattgcagaa gaaatactgt caggaaacat aatgcctgat ggaaattttc
      481 ttattcaaga gattcctctt ttcgcaccga atttggcttt aaacgacatt gttgccatag
      541 aacgtgagga taagatgctg tttttcgacc acttgataaa agcttcagga aataccacga
      601 taaacatcgt tgttttggat catttcccaa aggatttatt ggcagccata gaagaacaca
      661 gtggtaaaat cagaaaaaat ggagagaatt atttatcggt taattttccg cccaaaaaat
      721 ataattctga tttaaaagga attttaaata gatatgagga agcaaatatc ctcagctaca
      781 gggaagcttg tttgggcttc tcttaatcga tctataagta agaatggatt acgttagatt
      841 gtaaacctaa cgattaaaag gtcgtctgaa accgtgttta attttcagac gacctatttc
      901 cttcatttga aacaggatat tgggaattaa gtttttcaaa aatcctacac ctgctccttc
      961 cacggcagca ccttggtcaa aacggcagac ggctacaaag ccaatggacg tgtaatagaa
     1021 aatagtgcgg taacaattat tcgggattga agaatgatga gtgtaaaaga attattcaaa
     1081 gtcatcttag atgaaaataa agattttcct attagaacga ttcatcgtac cccgatgggg
     1141 atacttccca aacctgttgc tttaagtatt gttcaatatg aaaatgaccc gggattttat
     1201 ttgttttatt tggatgagac cggtcaggaa caaacggata cttatcatga cacattagat
     1261 tcggcatttg aacaggctga atttgaattt ggaatcagca aagaggaatg gatgcaaagt
     1321 ccttagacgg caaaatcaag atgccgcttt atttttcaga tggcatttcc gtcaataaaa
     1381 aagtcggtat tggagcccaa aacagcctac gtcttgccgg gatagggatg aaatctttaa
     1441 gaaaaaatga ttatagttga acatttagaa cattatttgg gtgaaatcga aagcggtata
     1501 aaatgcttgg atcgacgtta tcatctcagc gtttccgtct tcccatcaca accatataaa
     1561 ggggtaacta ctttttctac tttaggcttg aatcgatatg acttgaacta taagagtagg
     1621 tttgaattaa tttttacctg ttctgaagaa tggaataaag aaaatattgc agcttttcta
     1681 tcaggagtgg ctgaatattt gattgacaat agacaaccta ttttaagagg tgaaattatt
     1741 caattaccca gggtaattat cgaaggtagc aaaatggatg cactgtatgt ttccgcccca
     1801 ttttatttcg atgatgattt tcaggtctgt tatggcgaac actacaatat cgttttccct
     1861 ttgcttgtcc ccttgtataa acaggaagca gaattggtgg aaaagaaagg ttggaatgct
     1921 tttgagcagt tcttgctgga taatgaagtt gacaaccttt cggatatgaa gcggaaaccg
     1981 tttgcttggt aagggctttc ttaaatttga ttaaaggtcg tctgaaatta agcttgtttt
     2041 tcagatggtc tttccttcat ttgaagcagg atattgagaa ctgagttctt caaaaatcct
     2101 acacctgctc cttccacggc agcaccttgg tcaaaacggc agacggctga aaagcaaaca
     2161 ccgtccgtcg tgttgccgtt tgcggatgag tacgggtcaa ccccaatgcc gccgaaaccg
     2221 tcgaagccgc cttcaacatt gccaccgcca aagccgcgaa gttggcaaaa acggtaaaac
     2281 cggggagata aaagccgatg gcaggaaagt aaatgtgagg atagacagta cggaggcaga
     2341 cctgctttat ccggcagggc aataagaaaa caaaacttag atatggaaaa cgattgtgaa
     2401 gattaaacca ttacaatttt ctaacaataa tcacagattt tatgtggaca aaatttgtgt
     2461 taccgaacaa gatgtgaaca ttttggcatc cgaccgaaat tatgaattaa atattgaaat
     2521 atttattgac aatataattc attttcaaat aacggatgaa tcttataaag taaaattttc
     2581 agaatattta ttcgaaaata aaacagaaaa taattcggat gggaatccta ctgcaaatta
     2641 ttttttcgag ataatagatg acagttatat ggactggttg aaagaagaaa gttttggttt
     2701 ttttgaaaag aaatattata aggcttatat tttctttttt agcgattctg taatagaagt
     2761 tatcagctcg acagaacctg tattttattc aaaataacaa attatcaaac aaaactctga
     2821 ttaaaaaccc aacaatcaga atgccgtctg aagcggtatt cggctttcag acggcatttt
     2881 ctccgattgg cacaggatgc aggggcttgc cggcttgatt ttgccgaagt tgttggcacg
     2941 caatttggag ggggcgacaa cctatgaaaa ataaatcgtt aaggatattt ttagttttac
     3001 ccaatttgtt tccgaaaaag tacggcagca accgtagatt agattaaaaa tcaaacaatc
     3061 aaagcccgcc tgatataatc gatatatcct gttaaacgaa aaataaatat gtttaaaaaa
     3121 ttcaaaccgg tattgttgtc attttttgca cttgtatttg ccttttggct gggaacgggc
     3181 attgcctatg agattaatcc gcgttggttt ttgagcgata cggcaactga aatacctgaa
     3241 aatccgaata cttttgtggc gaaacttgcc cgcctgttcc gaaatgccga cagggcggtt
     3301 gtcatcgtga aggaatcgat gaggacggag gaaagccttg ccgaaactgt ggatgacggt
     3361 ccgttgcagt cggagaagga ttatctcgcg ctcgctatcc ggctcagtcg tttgaaagaa
     3421 aaggcgcaat ggtttcacgt aacggagcag gaacatgggg aagaggtctg gctggattac
     3481 tatatcggcg agggcggttt ggttgcggtt tcgcttttgc aacgatcgcc ggaagcgttt
     3541 gttaatgccg aatatctgta tcggaacgat cgtccgtttt ctgtaaatgt gtacggcgga
     3601 acggctcacg gggaaaatta tgaaacgaca ggagaatatc gggttgtttg gcaaccggac
     3661 ggttcggtat ttgatgcgtc ggggcgcggg aaaatcgggg aagatgttta tgagcattgc
     3721 ctcgggtgtt atcagatggc ccaggtatat ttggcgaaat accgggatgt cgcgaatgac
     3781 gagcagaagg tttgggactt ccgcgaagag agcaaccgga ttgcgtcgga ctcgcgcgat
     3841 tatgtgtttt atcagaatat gcgggaattg atgccccggg ggatgaaggc gaacagtctt
     3901 gtggtcggct atgatgcgga cggtctgccg caaaaagtct attggagttt cgacaatgga
     3961 aaaaaacgcc agagtttcga atattatttg aaaaacggaa atctttttat tgcacaatct
     4021 tcgacggtag cattgaaagc ggatggcgta acggcggata tgcagaccta tcatgcgcaa
     4081 cagacgtggt atttggatgg cgggcggatt atccgcgaag agaaacaggg agacagactg
     4141 cctgattttc ctttgaactt ggaagatttg gaaaaagagg tgagccgtta tgcagaggct
     4201 gcggcgagac gttcgggcgg caggcgcgac ctttctcact gaacgcaaat gccgtctgaa
     4261 accggttttc agacggcatt ggagagtcaa acgctttttg caatgccgta tatgccgaac
     4321 ccggttcgct acaaattgaa aaccacaatc caaaaaacgg aaatacacat tatcaaacaa
     4381 acaaataagt tgacatattg tgcgatattg agggctgtac cgtattttcc cgttctttat
     4441 atgagcggca ggcatcctca taaaagaagg gataccgata aggaaaaatc cctcggtata
     4501 aaaaatatac tgattaatca atacaaatcc aacaaacagt tcatcatttt cttttctgtt
     4561 ggcgtataaa aaccgctcgg aaaaaaataa aatatcaaaa cggcaaaata aaaatactga
     4621 tatatgcctt cactttcata gggcgacgct ctatgtgccg tcctgtgtgt tgaaacgtcg
     4681 gttttcctta ccgcctgccc cgcttttccc tgattgctgc caactgccgt ccgaacctga
     4741 accgtcaggt ttcagacagc attttttctc cctgttccca caattgccgt ttttgtgcgg
     4801 cggtcagttt tttgacggcg atttcctgtt tgagcgcagt gcctttatcc atgccgcctg
     4861 caacgatacg catcgccacc ggtttgaata cgcgggtata tttcgcgcct tttccggctg
     4921 tgtggatggc aagccgctgt tgcggattcg ggctgatacc gcaatagaac gcgctgtttt
     4981 cacacaatat caggtaaacg ctccagtttg acgcgttcat ctgcctgcat cctgggtttt
     5041 ttccccgttc caaggcgcaa ctttcagcag cagcagcata cccggcaggg caagtatgaa
     5101 acacagccgg aaaaacggta cataacccat ccattcgatc agatagcctg caaaggaatt
     5161 gatgaccgtg cgcggcacgg cggacaggct ggtaaacagc gcaagctgcg ttgccgtaaa
     5221 cgcgggattg gtctcgcgcg ccatatacga tacgaacgcc gccgtcccca accccacgcc
     5281 gaccgcttcc gcgccgataa ctgccgccag catcagcctc tcgcctgtgc cgaccgtgtc
     5341 gaaatgtccg aaccctgcca gccatacaaa ccccaaaacc gttattgcct gcaccgcgcc
     5401 gaatagccac aaggctttgt ttacgccgat tttcagcatc cacacgccgc ccaagatacc
     5461 tgccgccact gccggccaca gtcctgcatt tttggcaatc aaaccgatgt cggtcttgct
     5521 gaaacccata tccagataaa acggcgttgc caacgcggtt gccatactgt cgccgagttt
     5581 gtaaaggaag ataaacagca gcacgcacac cgccgaagcg atgcccttgc gcataaaaaa
     5641 ttctttaaac ggctctacca cggtctgctt caacgtttta ggaacggaag gcggcaacac
     5701 gggttcgtgc gcgagaaaca gcgtcatcag aaggccgggc agcataaata atgaagtgat
     5761 aacaaatact tctgaccacg gcatcctgtc tgccaacacc aaactcaatg aaccgggaat
     5821 cagggcggca acccggtagg cgttcacatg aaccgagttg cccaaaccca attcttcgtc
     5881 cgacaaaatc tcgcgcctga acgcatccaa tacaatgtcc tgactggcgg aaaaaaaagc
     5941 gacaagcacc gacaagccgg caatcaacgg cagatgattc cgggggttta aaaaggcata
     6001 tgccgccaaa gccgccagta accctgcctg cgtcagcagc atccacccgc gccgccgtcc
     6061 caaaacgggc agcctgaccg cgtccatcag cggcgaccac aaaaatttcc aagtaaacgg
     6121 cagaccgatc aacgccatca gcccgatgct cttcaaatcc acctgctcgc tgcgcaacca
     6181 cgccggaatc aggttaatca gaaagtacag cggcagcccc gaggcaaacc ccgtaaaaat
     6241 acagacgagc atattacggg aaaagatttg cccgataaaa cctgatttcg atgcagtcat
     6301 cgttcgtaca gccaaaaaac aaaagcctat tgtagcaaat atcgcccgcc tgctgaaaaa
     6361 tgccgccacg cgccgccggt ttgccgttcc aatgcaaaat gccgtctgaa actgtgttca
     6421 gacggcattg ctactgaaac ccgacggttt acaggctgta atacatttca aattccagcg
     6481 ggtgcggcgc catacggatg cggcggacat cttcctcttt aaaggcgatg tagctgtcga
     6541 tccagtcttt gctgaacacg ccgccgcgca gcaggaattc gtggtcggcc ttgagggcgg
     6601 caagtgcttc ttccaaagaa gcgcagacgg tcgggacgag cgcgtcttct tccggcggca
     6661 ggtcgtacag gtttttatcg gcagggtcgc ccggatggat tttgttttga atgccgtcca
     6721 aacccgccat cagcagggcg gcaaatgcca aatacgggtt ggcggtcggg tcggggaaac
     6781 gcgcttcgat gcggcgcgcc ttgctgctgt tcacagacgg gatacggatg gaagcggaac
     6841 ggtttttggc ggaataggcc aatttggtcg gtgcttcaaa gtgcggcacg aggcgtttgt
     6901 aggagttggt ggacggattg gtaatcgcgt tcagggcttt ggcgtgtttg atgatgccgc
     6961 cgatgtagta gagcgcggta tcggacaaac cggcatagcc gtcgcctgcg aacaggtttt
     7021 gaccgtcttt ccaaatggat tggtggacgt gcataccgct gccgttgtcg cccataatcg
     7081 gtttgggcat aaaggtggcg gttttgccga aattgtgggc aacgttttgg atgacgtatt
     7141 tcatatcttg ggtttggtcg gcgcgtttga ccaaagtggc gaaacgggtg ccgatttcca
     7201 tttggctgcc ggtaccgact tcgctgtggt ggacttcgac ttcgatgccg agtccttcca
     7261 aaatgttcac catggcggag cgcaaatctt gaccgcagtc aatcggcgcg acgggcgcgt
     7321 agccgccttt gacggcgggg cggtggccgg tgttttgacc gtccatatgc aggccgcttg
     7381 cccacgcgcc gctttcggac gtgatttcgt aacgggtttt gtgcatgtcg gtttcaaatt
     7441 ctacgccgtc gaagacgaag aattcgggtt cggggccgaa ataggcggtg tcgccgatac
     7501 cggaagattt caaataggct tcggcgcggc gtgcgatgga gcgcgggtcg cggtcgtaac
     7561 cctgaccgtc ggcagggtcg atgacgtcgc aggtaatgac gacggtaaca tcatcataaa
     7621 aaggatcgac gaaggctgtg gacgcatcgg gacgcagctg catatcggaa gcctgaatgc
     7681 ctttccagcc gccgatggac gaaccgtcaa acgcctgacc gttttcgaac cactcttcgg
     7741 ggccttccaa cacgatgcgc gcaggcacgg taaagtggtg ctgcttgcct ttggtatcgg
     7801 tgaagcgcaa atcgacaaag cgggctttgc ttttttcaat caattttacg gcgtttttaa
     7861 tggacatctt cagctcctga aaaagggaaa tggcaaaata cgcggaacgc ggcggcttga
     7921 cgcaaagtcg gcacgatttt accacgccga tgccgtctga aaccggtttt gcgtcaaatt
     7981 ccaagccgga aaatatagtt tcaaacggca agattgtcaa caatatgctt cggtttgtct
     8041 gttcaaaagc ggaatgcccg cccttttgtc cgaatgccga caaaacagat gccgtctgaa
     8101 ggcggtttca gacggcatcg ttcccatctt cgcgccgccg ttataaaatg accgccccgc
     8161 ctttatggaa tatgactatg cacgccctcc cccgctacgc cgttttcggc aaccccgccg
     8221 cccacagcaa atcgccgcag attcatcagc aatttgccct tcaggaaggc gttgacatcg
     8281 aatacggacg catttgcgcc gacatcggcg gtttcgcgca ggcggtttcg acattttttg
     8341 aaacaggcgg ttgcggggca aacgttaccg taccgttcaa acaggaagcg tttcatctgg
     8401 cggacgaaca ttccgaccgc gctttggctg ccggtgcggt caatacgctg gtgtggttgg
     8461 aagacggcag aatacgcggc gacaataccg acggtatcgg tttggccaac gacatcacgc
     8521 aggtcaaaaa cattgccatc gagggcaaaa ccatcttgct tttgggcgcg ggcggcgcgg
     8581 tgcgcggcgt gattccggtt ttgaaagaac accgccctgc ccgtatcgtc attgccaacc
     8641 gtacccgcgc caaagccgag gaattggcgc ggcttttcgg cattgaagcc gtcccgatgg
     8701 cggatgtgaa cggcggtttc gatatcatca tcaacggcac gtccggcggc ttgagcggtc
     8761 agcttcctgc cgtcagccct aaaattttcc gcgactgccg ccttgcctac gatatggttt
     8821 acggcgaagc ggcaaaaccg tttttagatt ttgcacgaca atcgggggcg aaaaaaactg
     8881 ccgacggact gggtatgcta gtcggtcagg cggcggcttc ctacgccctc tggcgcggtt
     8941 tcaaaccgga catccgcccc gtcatcgaac acatgaaagc cctgtaacca tgttccgcat
     9001 cgtcaaatgg ctgattgccc tgcccgtcgg catctttatc ttcttcaatg cctatgtgta
     9061 cggcaacatc attacctacc gcgccgtcgc gccccatcgg actgccttta tgtcgatgcg
     9121 gatgaagcag tttgaacaag aaggtcgcga tgtcgcactg gattaccgct ggatgcccta
     9181 caaacgcatt tccaccaacc tgaaaaaagc cctgattgct tccgaagatg tccgttttgc
     9241 cggacacggc ggcttcgatt ggggcggcat tcaaaacgcc atcaggcgca accggaacag
     9301 cggcgaagtg aaggcgggcg gatcgaccat cagccagcag cttgccaaaa acctcttcct
     9361 caacgaaagc cgcaactatc tgcgcaaagg ggaagaggcg gccattacgg caatgatgga
     9421 agctgttacc gacaaaaaca ggattttcga actgtattta aactcaatcg aatggcacta
     9481 cggcgttttc ggcgcggaag ctgcgtcccg gtatttttat aaaaaaccgg ccgcagacct
     9541 gaccaaacag caggcggcga aactgacggc actcgtcccc gccccgctct actacgctga
     9601 ccatccgaaa agcaaacggc tgcgcaacaa aaccaatatc gtgctcagac gcatgggttc
     9661 ggcagaatta cccgaaagcg atacggactg attgtttcag atatggaaat gccgtctgaa
     9721 ctgcggttcg gacggcatat gttttctggt tcttatagtg gattaaattt aaaccggtac
     9781 ggcgttaccc cgccttgccg tactatgttg tactgtctgc ggcttcgtcg ctttgtcctg
     9841 atttttgtta atccactaac ctgcccgaca gccgcaaaga ccatgtcgtg catggcaggt
     9901 atttgcggtg cgggactgct gccgaaaacg gcaggcaaaa aataaccgct tataagcggt
     9961 tttttggtgg ccagaggcgg aatcgaaccg ccgacacacg gattttcaat ccgttgctct
    10021 accaactgag ctatctggcc tgtatctgtc tcgttaagag gtgcgtatta aaccaaattt
    10081 cgggacgaca cgcaagcgga tttgtcgata ttttttaccc aaccgactaa gtcgatgttt
    10141 taagtttaaa ttaatttcac agaaaaatag tgcaaatcgg ttttttgccg ttcgttcggc
    10201 gatgttctgc tttgcagacg gcaaaacgct tcaatatttg aagttctcgc ccaaatagac
    10261 ggcgcgcacc tgttcgttgc cgaccaaatc gtcgggcttg ccggaagcca gcaccgcacc
    10321 gtctgaaatg atgtaggcgc ggtcgcagat gctgagggtc tcgcggacgt tgtggtcggt
    10381 aatcaatacg ccgatgccgc gcgatttgag gaaaccgatg attttctgga tgtcgatgac
    10441 ggcgatggga tcgacgccgg caaaaggttc gtccaaaagg atgaaacgtg gtttcatggc
    10501 gaggacacgg gcgatttcga cgcgccgccg ttcgccgccg gacagcgacg gcgcaggatt
    10561 gcggcgcaag tgtccgatat tgaggtcggc gagcagtttt tcgatttctc tgtcgatttg
    10621 atttttatct ttggttctga tttccaagat ggcgcggatg ttttgttcga cggtcatttt
    10681 gcggaatatc gaggcttcct gcggcaggta gccgacaccc aggcgggcgc gttcgtgtat
    10741 gggcaggtgg cgcaattccc gcccgtccag catcacgctg cctgcgtcgg cagcgatgag
    10801 cccgactatc atgtagaagc cggtggtttt gcctgcgccg ttgggcccga gcagtccgat
    10861 gacttcgccg ctttcgatct caagggagaa acttttgacg acttggcgtt ttttgaaact
    10921 tttctgcagg ttttggacga cgaggcggct gttgtttgcg ctcataaatc tcttctcggc
    10981 atttcggtcg gcaaatgccg tctgaactgc gtttccgttt cagacggcat cggggttatt
    11041 cggttttttg tgtgcttgaa ggctggatga cgacgctgac cctgccggtt ttggaagcgg
    11101 atttcgcacc cgatttcgtg ctgccgttga tggtatagac ttcggttttg gtgttgtagg
    11161 taatgaccgc accttcggca acgtcgccgc cgcgctgcac tttggcattg ccggtcagaa
    11221 cgacggtgct tcctgcggag gaataggtaa cgttgtttgc ctgaccgcgc accgtccctt
    11281 tgcccccgtc caacgtttgg ctgaagcgga cgggcgaacc ttccgccctc acggattcgc
    11341 cgcctttgcc gccgcgtgtg acgttgacgc gcgaggcgga aatgttgagc gtaccctgtc
    11401 tgatgatgac attgccgcta aatgtggtac tttggttggc ttgatcgagc gaaccttggt
    11461 cggcttcgat ttggatgggc cgtctgctgt cgctttgaag ggcaaaagcg ggagaagttg
    11521 caaaaattac aattaaaaca aatagcttac atatcttttg tatcataaat cgcggctttc
    11581 actttagatg agaagttcaa catgcctgtt ttgtggttgt aggtcatacc gcccgcctga
    11641 ccgtgcgacg cgccatattg gaaactgaca ggcgtatcgg tttgggcata ttgagattcg
    11701 gtatcgacgt gcagtttttc ggtttcgact ttacccgcct gccgcctgcc gtcggcggtt
    11761 ttggtcagca caacgttgtt tttaaaaaga acctgtttgt tttcggtatg gtaaacggct
    11821 tcatcgctgc cgacttcgta caacagcctg ccttcttgga agaacacgag atgcggcgaa
    11881 tcaaaatgga tgtcgctgtt ttcgggaaac tgtttcgcac ctttcgcgct caaatgttct
    11941 ttcaagtatc cctgttcgtc aaaccgcctt ccgtccaagc cgtccattgt gtattgaggt
    12001 tcgtcgggat tgagcctgac ttcctcgatt tcgacttcgc tgatacggcc caaccatgcc
    12061 gacaggctgc ccaaggcaac cgccaatatc aatgggaacg caattccgta ccgccatctt
    12121 actttcattt gatgtacccg ttcaaagccg cgcccaacgt acctttcgcg cggataatca
    12181 ggtcacacac ttcgcgcacc gcgcccgcac cgccggggcg tttggcaata taggcggcgt
    12241 gttgcagcgt aaaccaatgc gcgtcgggga cggcaaccgg caatccgcag cgcaccatca
    12301 ccggcaaatc gaccacgtcg tcgccgacaa aggcgcactc ggcttcttcc aagcctgcct
    12361 gcgcgcgcag ctcttcatac gcggcgcgtt tgtccgaaat gcctttgaag tagtaattta
    12421 tgcccaactg tttgacgcgg atgccgacgg agggcgcgtc ccgacctgtg ataatcgcag
    12481 tctgcacgcc gcttgcctga agcattttca ggccgtgtcc gtccagtgtg tgaaacgatt
    12541 tgatttcttc gccgtcgtcg cggataaaga tgcgcccgtc ggtcaaaacg ccgtccacat
    12601 ccaatatcag cagtttgatt ttggcggcgc gtgcctgtaa ttcgggagaa attgcctgca
    12661 ttgggatttc cttgttttca gacggcatat cttaccacaa atgccgtctg aaacggctgc
    12721 aacgccttat acaatccgcg ccgccaaaag gtcgtgcata ttcagcgcac ctgtcagcac
    12781 gccgtctgca tcggtaacca gaagcccgtt cacatggttt gcctgcatga ctttcagggc
    12841 ttcggtggcg aggcgttcgg cggagatggt tttagggtgt gtatgcatga cttcgtctat
    12901 cgaaagaccg gtaaaattgt cgcattcttg aaacaggcgg cgcaaatcgc cgtcagtaaa
    12961 cacgcctttc agacagcctt gcccgtccgt taccgccaac atgcccaaac ctttctcgct
    13021 catgctgacg atggctcctt tcaagggcgt gccgagtcgg acggcgggca ggccgccgcc
    13081 tttgtgcata atgtcggcaa cgcgcaaaag caggcgtttg ccgaggctgc cggcagggtg
    13141 gatcaaggcg aagtcgtcgg gcgtgaacgc gcgggcgcgc agcaggacga ccgccaacgc
    13201 gtcgcccaaa gccataacgg cggtggtgct ggtggtcggg gcaagcccca acgggcaggc
    13261 ttcttgcgaa accgatgcgg tgatgtggat atcggcatgg cgcgccatgg ttgaatcggg
    13321 gcgggcggtg atgcagacga gggtaatgtc tttgcgtttg agcgcgggga tgatggcggt
    13381 gatttcgtcg ctttcgccgg aattggaaat ggcggcgacg acgtcgttgt ccacaatcat
    13441 gcccaaatcg ccgtgtgccg cttccgcagg gtggacgaaa aacgcgggcg tgccggtcga
    13501 ggccatggtt gccgccattt tgcgcccgat atgtccggac ttgcccatac ccgtgatgac
    13561 gaccctgccc ttgcagtgca acaacgcgtc tgccgcaagg acgaagtttt cgtccaattc
    13621 cgccgcaatt tcgcgcaagc cttccgcttc ggtgtgcaac acttcgcgcg cccagtcgag
    13681 atatttttcg ttttctgcca ttgccatccg cctttgctgt aacaaaacta cccaaaattt
    13741 ccgaatcggg gtagcataag ccttcaaacc ccgcccatcc atccaaagga atcaaaaatg
    13801 actattttat cggacgttaa agcattagga caacaaatct ggctggacaa cctttcccgc
    13861 tcgctcgtgc aaagcggcga attggcgcaa atgctcaaac aaggcgtgtg cggcgtaact
    13921 tccaatcccg ccattttcca aaaagccttc gccggcgacg cgctttatgc cgacgaggtc
    13981 gccgccctca agcggcaaaa cctcagcccc aaacaacgct acgaaaccat ggcggttgcc
    14041 gacgtacggg cagcctgcga cgtttgcctt gccgaacacg aatccaccgg cggcaaaacc
    14101 ggtttcgtca gtctcgaagt ttctcccgaa cttgccaaag acgctcaagg cacggtagaa
    14161 gaagcccgcc gcctccatgc cgccatcgcg cgtaaaaacg ccatgattaa agtgcctgcc
    14221 accgacgcag gcatcgatgc gctcgaaacc ctcgtttcag acggcatcag cgtgaacctg
    14281 accctgctgt tctcacgcgc ccaaaccctc aaagcctacg ccgcctacgc gcgcggcatt
    14341 gccaaacgct tggcagccgg acaaagcgtt gcccatatcc aagttgtcgc cagcttcttc
    14401 atctcgcgcg tggacagtgc gctggatgca acgctgcccg accggctcaa aggcaaaacc
    14461 gccatcgccc ttgccaaagc cgcctatcaa gattgggaac aatatttcac cgcccccgaa
    14521 tttgccgcac tggaagccca aggcgcaaac cgcgtgcagc ttttatgggc atctaccggc
    14581 gtgaaaaacc cagcctatcc cgacacgctc tacgttgaca gcctgatcgg cgtgcacacc
    14641 gtcaacaccg tccccgatgc cacgctcaaa gcctttatcg accacggcac ggcgaaagcg
    14701 acgctgaccg aaagcgcgga cgaagcacgg gcgcggctcg ccgaaattgc cgcgctcggc
    14761 atcgatgtcg aaaccttggc ggcgcgtttg caggaagacg gtttgaaaca gtttgaagaa
    14821 gcctttgaaa aactgctcgc gcctttggtt taggcatttc cccgatgccg tctgaaaggc
    14881 tttcagacgg catcgccctt ttgaccggac acagtgcggc atatcaagga tccgccctaa
    14941 aaatattaat gggcaagaag ttaatgcacg acattacccg ccaacacaaa aggaaataga
    15001 atcatggcaa aagcactcga aatcatttca cccgatgaaa tttattcaga tctgattttt
    15061 aaggatccgg tacctcccca tactatatat acagaattga tgaaactggt tggtagaaat
    15121 gcaggcaatg agagaattgc agatagagcc ttcgattttt tctcgcctgc tcttatggat
    15181 gattcagcta ccgaagagca atataatgcc ctatatgatt taaccttgct tgaagagccc
    15241 gggatggaac tggataaaga tgagataacg gctttgatta atagtcttaa ataaaggggc
    15301 tgtaccggat atgtacacag gacgcttcgc ccccagcccg accgggttgc tccacatcgg
    15361 ctctcttctg accgccgtcg cttcctatgc cgatgcacgt tcaaacggcg gcaaatggct
    15421 ggtccgtatg gaagacctcg atccgccgcg cgaaatgccg ggggcggcaa gccatatcct
    15481 gcacacgctc gaggcattcg gattcgagtg ggacggcgaa gtcacctatc agagccgccg
    15541 ttacgccctg tatgaagaaa ccctataccg tctgaaaacc gccggactgg tctatccctg
    15601 ccattgcaac cgcaaagact ggcaggccgg ggcaaggcgg ggcacagacg ggttcgtcta
    15661 taacggacgc tgccgccacc ccggccaacg ccctgcactg caaggcaaac agccgtcgtg
    15721 gcgcatccgc gtccccgacc gcgacatcgg cttttcagac ggcatcgtcg gcagttacac
    15781 ccaaaacctc gcccgcgaca tcggcgattt cgtcctgttc cgtgcagacg gttactgggc
    15841 ataccagctc gccgtcgttg ccgacgatgc cgaacagggc gttacccaca tcgtgcgcgg
    15901 acaagacctg ctcgtttcca caccgcgcca aatctatttg cagcagtgtt tgggcgttcc
    15961 gacaccgcaa tatgcccacc tgccgctgct gaccaacgcg caagggcaga aatggtcgaa
    16021 gcagacgctc gcccccgcat tggattcaaa ccgccgcgaa caacttctcc gccaagtgtt
    16081 ccgctacctc aagctgcccg aagcaccgga aaccgaccgc cctgccgaat tgctcgactg
    16141 ggcggtggca cactgggata tggacaaagt gccgaaacac gccattaccg ccccctaaac
    16201 accacccgac aaacgcacaa atgccgtctg aaaccttcag acggcatttc acacatacag
    16261 ccccgcccta ttcgcgcgta tttgcccgtt cgacttcttt ccacgcttcg aaaatcaggc
    16321 cgccgtcgtt gtctttcaag agcgttgcat cggaaagcat acgccgccaa gtccgcgcgc
    16381 ctttcaaacc gtgcatcagc ccgaggctgt ggcggacgat gtggcgcagg attgtgccgc
    16441 gtccggcttg gatttggact tggctgtatg tgtagaggcg ctgcaccaaa tcggcgtatt
    16501 cgatcggggc gcggttgtca ccgtaaaaca gcctgtccca ttcgcgcatc accatcgggt
    16561 tgtggtatgc ctcgcgcccg accatcacgc cgtcaacgtg ttgcaagtgt ccggcgattg
    16621 cttcgttggt ggtgatgccg ccgttgatga tgatttccag ctcgggaaac tcctgcttga
    16681 ggcggtaaac gtaatcgtat ttcaagggcg gaacatcgcg gttttctttg ggcgaaagac
    16741 cgtccagcca agcgttgcgg gcgtggacga tgaaggtttt gcaggcggtt ttgtcgcgca
    16801 gcgtgccgac gaaatcggca acggtttggt attcggtctg cctgtccaca ccgatgcggt
    16861 gtttgacggt aacggggatg ccgaccgcgt cgtgcatggc gttgaggcag tcggcaacca
    16921 gcccgacttc gttcatcaga cacgcgccga acgcgccttt ctgcacgcgc ggactgggac
    16981 agccgcagtt gaggttgacc tcgttgtagc cgtattcttc ggcggctttg gcggctttcg
    17041 ccaaatcgga cggatcgctg ccgcccagtt gcagggcgac gggctgctcg ccttcgttaa
    17101 acatcaaaaa acggtctttg tcgccgtaaa caatcgcgcc ggcgttgacc atttcgctgt
    17161 acagccaagc atttcgggta atctggcggg caaggtaacg gtagtgcctg tccgtccagt
    17221 cgagcatcgg ggcgacgcat aaacgccttt tccctttatg ttcaataagt tccttgttta
    17281 tcataaggtt ttcacattct tttaactcaa acattcttta ttatttgctt ggatttttgc
    17341 ccatttcact atatttgacg taccatactt gcaccataat ttttaattct tggaatggtg
    17401 caaatggcaa caataaccaa gcggcgcaat ccttccggcg aaacagtata tcgggttcag
    17461 gtacgggtcg gcaagaaagg ctatcctgct ttcaatgaga gcaggacgtt cagtaaaaag
    17521 gctttggcgg tcgaatgggg gaagaaacgg gaggcggaaa tcgaagccgg tccagaactg
    17581 cttttcaagc gcggcaaggt caagatgatg acgctgtccg aagctatgcg gaagtatctt
    17641 aacgaaacgc ttggagcggg tcggtcaaag aaaacgggct tgcgttttct gatggagttc
    17701 ccgattggcg gcatcggcat cgataagctg aaacggtctg atttcgcgga acacgttatg
    17761 cagaggcggc gcggaatccc cgaactggac attgcgccaa tcgcggcttc tacggcattg
    17821 caggagctgc aatatatccg ttccgtgcta aaacacgcct tttatgtgtg ggggcttgaa
    17881 ataggctggc aggaattgga tttcgcggca aacgggctga aacgctcgaa tatggttgcg
    17941 aaatctgcaa tcagggacag attgccgacc acggaagaac tgcaaaccct gacaacttat
    18001 ttcctgcggc aatggcaaag caggaaatct tccataccga tgcacctgat tatgtggctg
    18061 gcgatttata cgtcaaggcg gcaggatgag atttgccgcc tgctgtttga cgactggcac
    18121 aaaaatgatt gtacccggcc ggttcgtgat ttaaaaaatc cgaacggcag cacagggaat
    18181 aataaggagt ttgatattct gcctatggct ttgccggtca ttgacgagct gccggaggaa
    18241 tcggtcagga agcgtatgct tgccaacaaa ggcatcgccg acagccttgt accgtgcaac
    18301 ggaaaatcgg tttccgccgc ttggacgagg gcgtgcaagg ttcttggaat taaagacttg
    18361 cgcttccacg atttacggca cgaggctgct acccgtatgg ctgaagacgg cttcacgatt
    18421 ccgcaaatgc aacgggtaac gctgcatgac ggttggaaca gcctgcagag gtatgtgagc
    18481 gtacgcaaac gctcgacgcg gctggatttt aaagaggcaa taatgcaggc gcaaagcgat
    18541 ataaaatccg ggaagtgata ttaaattaaa ggggatgtgc cgcatcccct gattcctccg
    18601 tcttgcatca agaccggaat ttccgatatt ccgattttgc cttttcctcc ttgtctgcga
    18661 ttgcctttgc aagctctcgg acgctgacga aatattccga ttttcggctg tcggacaatc
    18721 ggaaaatcgg aaacggaagc tcgcaccgta cccccgatgc tttaaacgcg ttaaagccga
    18781 tatgggcaaa aaagtctttg tgtacttccc tcggcgggat atacgggcgg ccgccgtatg
    18841 cgattaaaag cccttgttct ttactgatgt tcatatatac ctctttctgt taattctgct
    18901 ttgtctgagt ttccgctttt ttacggcttg gcggatgttc agcctgcgtc ggtgcataag
    18961 tcctcctgtt cgggcggtta ttcagccggc cgcccctccg aaaagtggca ggctgcacgg
    19021 gaagtagtgt ttttttccgt atttctctgc ctgtatcagg ttgcacaggc ggtcgcaaaa
    19081 gccgtcaaat tcggcatgcg gccagccttc tatctcgttg accattttgc tgaacctcaa
    19141 ttcccgggca atccgctttg ccgctgcgtc acggcattcg tgttccaaga ggacggggtc
    19201 ttccttttcc ttcggcggtg caggttcttg cacggtttgc cacaagtccg gttcgatttg
    19261 ccaagtgtcg gcggcaagca gggtgtctgt tttgcagtcg tacagttcgc ggatgcagcc
    19321 gccgtcggtt ttgtctttgt cgataaccag taaaagcact tcgatcggcg tgtcttcaaa
    19381 cgcgttttgt atgcggttga gttctgccag ccggttgccg atgatttcgc ggaatcgctg
    19441 ctcgcttttg cggtaggcga tgccggggaa caggatgaag aatccgaagc ggcgggcgtt
    19501 ttccaatcct tttaaaacga agatttcatc ggctacgccc gatttcttcc acgggtattc
    19561 ctgtgcgata cggctttttt cgtcttcgct caaatccttt agcttgatgg aaaacggcgg
    19621 attcatgacg gtgcagtctt gcggttcgcc gtcggtataa aggaaaaggc ttgtgttgta
    19681 tactttggcg gcagggtagt tttgcaacaa ggctttgcac gcttccgcct gtatctctac
    19741 ggcgcggaaa tcggacggtt cgatgtactg ctccagctgc ccgcttcctg ccgcgccgtc
    19801 aaatacgccc gggtgttcgc cgcagtatcg gcggactttg gcggcaacca gccgcctcaa
    19861 gc
//
LOCUS       DAXDZG010000039        19726 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXDZG010000039 DAXDZG010000000
VERSION     DAXDZG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19726
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.039"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            145..2393
                     /locus_tag="ACFB78_001203"
                     /pseudo
     CDS             145..2393
                     /locus_tag="ACFB78_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2518..4068
                     /locus_tag="ACFB78_001204"
     CDS             2518..4068
                     /locus_tag="ACFB78_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC1269195.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="ACFB78_001205"
     CDS             complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="ACFB78_001205"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1269196.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6294..6728)
                     /locus_tag="ACFB78_001206"
     CDS             complement(6294..6728)
                     /locus_tag="ACFB78_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269197.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="ACFB78_001207"
     CDS             complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="ACFB78_001207"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC1269198.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="ACFB78_001208"
     CDS             complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="ACFB78_001208"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC1269199.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11901..13613
                     /locus_tag="ACFB78_001209"
     CDS             11901..13613
                     /locus_tag="ACFB78_001209"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC1269200.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13676..14332
                     /locus_tag="ACFB78_001210"
     CDS             13676..14332
                     /locus_tag="ACFB78_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC1269201.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14342..14797)
                     /gene="ruvX"
                     /locus_tag="ACFB78_001211"
     CDS             complement(14342..14797)
                     /gene="ruvX"
                     /locus_tag="ACFB78_001211"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC1269202.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14790..15338)
                     /locus_tag="ACFB78_001212"
     CDS             complement(14790..15338)
                     /locus_tag="ACFB78_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC1269203.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15340..15900)
                     /locus_tag="ACFB78_001213"
     CDS             complement(15340..15900)
                     /locus_tag="ACFB78_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC1269204.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGASFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16226..18028
                     /locus_tag="ACFB78_001214"
     CDS             16226..18028
                     /locus_tag="ACFB78_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC1269205.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKA
                     QISRFVSGNYKNSRPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKG
                     TEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEKEAAQQKAEARRAE
                     MSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVF
                     YSTAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAG
                     SKIGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18155..19630
                     /locus_tag="ACFB78_001215"
     CDS             18155..19630
                     /locus_tag="ACFB78_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC1269206.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
BASE COUNT         4870 a         4841 c         5370 g         4645 t
ORIGIN      
        1 caatatttaa cagtcggata gctccgcctg attaagtttt tcggtaatag ttgtgattat
       61 caaatataat tgccgcccga aatatccgat aagtttgttt gttttatctt taattcccca
      121 atacttaaaa aagagagcgt tttcatgaac aaaaaacttg ccttaatgcc gctgttgatt
      181 ttgagcgcgt tttcatctgc cgccgacaat actccgcagc atggcgaact cggacaggtt
      241 catgtccgcg ccgatgccaa gcgcgtcaaa gccgcgcatt cttactccat cgccagcgat
      301 ggcgacttgc gcgaccgcgt aaatttgggc gtattgggta aagccaacgc gtttaccgcg
      361 ccgattaccg tcgtcaacta cgacgaacaa gccttcaaca ataccgaggc gcgtacttta
      421 gtggatgccg tagccaaaaa agacgcttcc gtttgagagt ttggcggcga aagcaacacg
      481 ctgaccggtc tgtatttccg tggttatcag cttgatgcgc gccaattcag cgtaaacggc
      541 ttggcaggaa tgtatggcac gcaaggcacg gcgagcgtgc aagtcggctt cgcacaactg
      601 attaaaggcg cgtccaccgc cgtaaacggc ataccctgaa ggcgcggtat ccggttccgt
      661 caacatcgaa accaaaaaag ccgccgacga aggcaaccgc aaaatcggtt tggcccggtt
      721 cggcaacaac cgcgcccaag gtaggttcga cttagggcaa cgcttcggcg aaaacaaagc
      781 attcggcgtg cgtgccaacg gcaaactgcg ccacggcgac accccgcgcc acggttacag
      841 agaagacaac aaagaatttg ccttaaatgc cgattatcgc ggcgaaaaac tgcgcgtgac
      901 gttcgactcc atctacgcga aacgcaaaat caacggcggc cgcgcgcgta tgaaggatat
      961 ccaaaacgcc ggcggacgct tgttcgacgc gcccgacggc aaaatcaacc tgctgccttc
     1021 ttggaactgg caaaataccg tcggcgaaac caatatgctg acgtttgaat gggatgcgtt
     1081 tgacaatacc taaattaccg gcggtatcgg ctacaacaaa gcgcgttatt acggtacgct
     1141 gatttcccca accgtttgcg gtacaagcgg tgcgagcagc caaaccaaaa cctgcatggc
     1201 tgccaaccaa taccacaccg gcacggcgcg cctgaccgac caatatttcc gtactttgag
     1261 tatgaacctg accgcacgcg gcgaatttga aaccggcccg gtcacgcata actggagtac
     1321 ggctttcgac cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag
     1381 caacagcaag atagaagtga aggcaaacga aaatattgcg aatcaattgg catcgttcaa
     1441 agccgactat cccaattctt gggcgaactc ggccaacttg gatgccaata tcaaagtcaa
     1501 cagcttggct ttgtccgata ctttgggatt cgttgataac aaataccctc tgactttggg
     1561 cggacgtttc caagcggttg aatacactga taagaagaaa tcacagagcg gcgatgccaa
     1621 acgtttcagc ccgatgctga tggcggcgtg ggtgccgcaa cctgatttgg tcgtgtgcgg
     1681 caactatatg gaagacttgg agcgtgccga tatcaaaacc gatgattccg gcgaaacgac
     1741 aatggcgaag ccgcgcgtca gccgccagtt tgaaatcggc gtgcgtaaaa actggggcga
     1801 ttttgtaacc acgttgaacg cgttccaaat caaacgcccg ggctactggc gcggcaatac
     1861 caaaaaaggt actgattttg cagcatataa agcagcagga ggcgcagccg gcgacgagca
     1921 aggtatggaa cgcaatcgcg gtattgagtt caatgcctat gccaacctgt tgaacaaaac
     1981 cctgcgcccg actttcggcg tgatgtatct gcaatcgtcc gtgaagaatt atccgaatgc
     2041 gcgcgatatg ctggttaacg gcgtacaagt cgccaacccg cgcgtgattg tcaaaacagg
     2101 cgttgaacgg gatacaccgt ttgccaaagg tttgagcttg aacggcaatg tttcgtattt
     2161 cggcaagtct tatcaagaca cgcaaaaaca atacgccttc ccgtcctata ccttggttga
     2221 cgtaggcgcg ctacaaaacc aagctgggca aaaacaccct gaccgtcagc agctcggtag
     2281 aaaacctgtt caacaaaaac tactggcagg tacagcgcgg acaatttgac cgtagtttcg
     2341 ccgtcgtcgg tctgccgcgt acttactggc tgaaggcgga attggatttc taagctgcct
     2401 ccgtttccga aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agaaatatgg
     2461 cgggatatgc ttaaataatg ctattctagt acttttgaaa cctgcttgtt tgaagtgatg
     2521 aaaaaacctt tgatttcagt tgcggcagta ttgctcggcg ttgctttggg tacaccttat
     2581 tatttgggtg tcaaagcaga agaaagtctg acgcagcagc aaaaaatatt gcagaaaacg
     2641 ggctttttga ccgtcgaatc gcaccagtat gatcgaggct ggtttacctc tacggaaacg
     2701 acggtcatcc gtctgaaacc cgagttgctg cataatgcgc agaaatacct gccggataac
     2761 ttgaaaatag tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc
     2821 gccggcggat tcggcacgca ggcgcacatt gaaaccgagt tcaaatacgc gcctgaaacg
     2881 gaaaaagttt tggaacgctt ttttgggaaa caagttccgg tttcccttgc caataccgtt
     2941 tatttcaacg gcagcggtaa aatggaagtc agtgttcccg ctttcgatta tgaagaactg
     3001 tcgggcatca ggctgcactg ggaaggcctg acgggggaaa cggtttatca aaaaggtttc
     3061 aaaagctacc gcaacagcta tgatgcgccc ttgttcaaaa tcaagctggc agacaaaggc
     3121 gatgccgcgt ttgaaaaagc gcatttcgat tcggaaactt cagacggcat caatccgctt
     3181 gctttgggca gcagcaatct gactttggaa aaattttcgc tcgaatggaa agagggtgtc
     3241 gattacaacg tcaaattgaa cgaactggtc aacctcgtta ccgatttgca gatcggcgcg
     3301 tttatcaatc ccaacggcag catcgcacct tccaaaatcg aagtcggcaa gctggctttt
     3361 tcaaccaaga ccggggaatc gggcgcgttt atcgacagcg aagggcggtt ccgtttcgat
     3421 acgttggtgt acggcgatga aaaatacggc ccgctggaca tccatatcgc tgccgaacac
     3481 ctcgatgctt ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa
     3541 atgactgagg aacaaatccg caatgatttg attgcggcag tcaaaggcga tgcttccgga
     3601 ttatttaccc atgacccggt actaaatatc aaaattttcc gtttcaccct gcctcaggga
     3661 aaaattgatg tgggcggaaa aatcatgttt aaaggcatga agaaggaaga tttgaaccaa
     3721 ttgggactga tgttaaagaa aaccgaggca aacatcagaa tgagtattcc tcaaaaaatg
     3781 ttggaagatt tggcggtaag tcaggctgga aatattttca gtgtaaatgc cgaagatgag
     3841 gcggaagcca gagcaagcat tgccgatatt aatgaaacat tgcgcctgat ggtggacagt
     3901 acggtccaaa gtatggcaag ggaaaaatat cttactttag acggtaatca gattgatacg
     3961 gtcatttccc ttaaaaacaa cgccctgaag ttaaacggga aaacgctgca aaatgaaccc
     4021 gatcctgatt ttgacgaggg agatatggtt tccggccagc cgcattaatg ccgtttatgc
     4081 caaatgccgt ctgaaatgtt cagacggcat ttttgtcggt ctcgaaatag gatggatttt
     4141 catgtttcaa aaacaattga ttgtctctaa tccgatatca ggacacgctt ccaatgttta
     4201 gatatggaat tcggacggga taggggaact attgaaatcc agttgaaatc tgttattgga
     4261 aagcatggag gaaaaataaa aggctgtatt ttaatatagt attgataggc atgcttagct
     4321 tcaaggtatt caatagtgta ggtgtctgcc taactggaaa ataatcgtct tttcccttgc
     4381 aaccagttaa aattgacaga tggttaaaac acagccaaat aaaaatgccg cctgaagggt
     4441 tcagacggca tttgttcgga taaaccttat cggctgctta gagcatcagg tttatttttt
     4501 cttttgagga ggcaggtcgg tacatacacc caaagccact tccgcagcca taccgatgga
     4561 ttcgcccaag gtcgggtgcg ggtggatggt tttgccgatg tctgccgcgt cgcagcccat
     4621 ttcgatggca aggcagactt cgccgatcat gtcgccaccg ttcggaccga caatgccgcc
     4681 gccgatgatg cggccggttt cggcatcgaa aatcagcttg gtaaagccgt tgtcgcaacc
     4741 gttggcaatc gcacgaccgg aagccgccca tgggaagttg gctttggtga ttttgcggcc
     4801 tgatgctttg gcagacaatt cggtttcacc gacccatgcc acttcggggg aagtgtaggc
     4861 aacgccggga ataacgcgtg cgtcgaagta ggctttgtgg ccggcacagt tttcagcggc
     4921 aacgtggcct tcgtgaacgg ctttgtgtgc caacatcggt tgaccgacga tatcgccgat
     4981 agcgtagatg tgcggtacgt tggtgcgcat ttgtttgtca acttcgatga agccacgatc
     5041 ggtaacggca acaccggctt tttctgcgct gatgagtttg ccgttaggcg cacggcctgc
     5101 cgcgaccagt acggcatcgt aacgttgcgg ctctttaggt gcatttgcac cttcaaaggt
     5161 aacgtaaacg ccgtcttctt tcggctcgac tgcaacggtt ttggtgttga ccataatgtt
     5221 gtcaaaacgg tattcgtttt gtttttgcca tactttaacc aagtcgcggt ctgcgccttg
     5281 catcaggccg tccatcattt caaccacatc caggcgagaa cccagcgtgc tgtaaaccgt
     5341 acccatctcg aggccgataa tgccgccgcc gataatcagc agtttgcccg gtacttcttt
     5401 cagtgccagt gcgccgctgg aatcgatgat gcgcggatct tcaggaatga aaggcagttt
     5461 ggttacgcgg ctgcctgctg caatgataca gtttttgaag gcaacgattt ttttctcgcc
     5521 ggtaggggct gcctgttcgt acgcgtcgcc ggcagtcagc gacacttcca agtggtgagg
     5581 atccaagaat tgcccgtcgc cttggataac gtccacttta cggcttttcg ccatacctgc
     5641 caaaccaccc gtgaggcggg aaaccacgcc gtctttgtag gcgcgaagca tatcgatgtc
     5701 gagttccggt tcggggtatt tgataccgtt ggcagccaag tggcgcactt cgtcgataac
     5761 ggcggcattg tgcaacaagg ctttggaagg gatacagccg acgttcaggc aaacgccgcc
     5821 caaagttttg taacgctcga cgatggcgac tttcaagcct tcatcggcag cggcaaatgc
     5881 agcggagtaa ccgccgggac cgccgcccaa tacgaccacg tcgtactcgg catcggcagc
     5941 accgccgaat tgcgcggctt gcggagcagg agcggcagct ttgggtgctt cttgcgccgg
     6001 ggcggcagcc gcttcggctt taggggcggc agccgcacct tcggcttcga cgacgacaat
     6061 caaaccgcct tcggagattt tgtcgccgac tttgactttc acttctttga tcacgcccgc
     6121 aacttcggca ggcacgtcca tcgtcgcttt gtcggtttcc aaggtaatca gggtgtcgtc
     6181 aacggcgatg gtgtcgccaa cgttcacttc aaccgcgata atatctacat tttcgtgtcc
     6241 gccaatgtcg ggcactttca attcaactaa agccattata ttgacctttc aacttagtta
     6301 atttctatca actctttagc aataatcact tcttcaggaa tacagccaca atcttcagct
     6361 ctgattgtta cttccttctc atctgggttt tctggatgtt caacctcata ttcaaaaata
     6421 gaatattgac caaacaaatc tctatttaaa acatatatat agccattttc gatgccggag
     6481 cttgttgcaa atttcttggc aatttccttg tctgttgtcg tagatatata acatccgtca
     6541 tatagatctg tttcaatttg atgggcgtaa actgcattct tcacacttgg accatgtgta
     6601 gctttaccat catatttaaa cttaccatca taacgaattg caacttcagc tttattacct
     6661 ttaggtttta actgtccatt attttgctca tcttgttggc aactaatgcc tctatataaa
     6721 taagaaattt cccataagtt attaatttaa agtttgaatt taacaccatc actcatcaat
     6781 ctcaagcaat ccaaacatta tcagactgct tgagagggat gttttatttt ataaggtaat
     6841 gcggcggaag tctttcaaca ggttcgccag gaatacggtg aagcgcatac cggcggcacc
     6901 gtcgatgacg cggtggtcga aggacaggct caacgggcac atcaggcgcg gggcaaactc
     6961 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatag cgacttcggg
     7021 agcgttcaca atcggcgtga aacctgtgcc gccgatgccg cccaagctgg aaatggtaaa
     7081 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tggacaattc
     7141 ggtcaattct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     7201 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     7261 gttgtcgccg tccaaagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     7321 tttgatgatg aacgccaacg gggacagttt cacgccttcg cgttcccatt ctttgttcag
     7381 ctgtttgcgg aattcttcca gctcggtcat atccgcttct tcgtgtacgg taacgtgcgg
     7441 aatcaccacc cagttgcggg acaggttttg accggagatt ttcttaatgc gggacaattc
     7501 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     7561 accgccgccc aaagatgcgc cggctgcggc aggttttgcc gcgccgccct gcattacgga
     7621 tttcacaaag gctttgatgt cgtctcccat gatacggcct ttcaagccgc tacctttgac
     7681 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     7741 tttggcgaaa gcggcttcat cgattttggc agcggcaggc gaggcagatg taggcacggc
     7801 ggcgggtgcg gcagcttgag cggcaggcgc aggagctgct gcggcagagc cggcggtttc
     7861 cacttcgata atggcagagc cttcggacac tttgtcgccg acttttaaga atacggcttt
     7921 aacgacaccg gcagcggtac aaggtacgtc cattgtcgct ttatcggttt ccaaagtaat
     7981 cagcgtgtcg tcttcggcaa cggtgtcgcc aactttgatt tcaacggcga ttacatctac
     8041 atcggtatgg ccgccgatat cgggaacggc tacttgaacg gttgcaccgc ctgcggcagc
     8101 gggtgcaggt tgtgcttcgg cggcagcagc cggggcggct tcggcagcgg cggcaccggt
     8161 ttcaacggtc aaaattacgc cgccttcgga gattttgtcg ccgactttga cttttacttc
     8221 tttcacgaca ccggccgcat cggcaggcac atccatcgtg gctttgtcgg tttccagtgt
     8281 aatcagggtg tcgtcaacgg cgatggtgtc gcccgcttta acttctacgg cgatgatgtc
     8341 gacgctttcg tgaccgccga tatcggggac tttgatttct acgatactca tttgagttcc
     8401 tttaatcttt ttcagttgga tgccgtctga aagctgcttc aggcggcatc ggtacggttt
     8461 gtctgttcaa accattcggg cattcggttt tcagacggcc tgatgttggc atgaaacaaa
     8521 caggccgtct gaaacatcaa tcagcgtttc cagctagggg ctgaatcggc tttgatgccg
     8581 tatttctcaa tggcttgttg aacggtttct ttgctgactt tgccttgttc cgccaatgcg
     8641 gccaatgctg ccacggcaac gttgtagcgg tccacttcaa agaagcggcg caggttggcg
     8701 cggctgtcgg aacggccgaa accgtcagtg cccaagacat ggtagtcgtt cgggatgtac
     8761 gcgcggatac ggtcggcata gctgcggata tagtcggtag cggcgataac cggaccgtca
     8821 tgaccttgca gttgggaagt aacgaaaggc actttttcag cttccagcgg atgcaggcgg
     8881 ttgaagcgtt cggtttcgat ggcgtcgcgg tgcagcaggt tgaaggacgg gcaagaccag
     8941 atgtctgctt ccacgccgaa gtcggctttc agcagctcgg caccggcaat cacttcttgc
     9001 agaatcgtac cggaacccat caattggact ttcttgtcgc ctttgccgcc ggctttcagc
     9061 aggtacatac ctttgaggat gtcttgttcc gcaccttccg gcatatcggg atgggtgtag
     9121 ttctcgttca tcagggtgat gtagtagaac acgtcttcat tattggcata catacggcgc
     9181 agaccgtctt gtacgatgac ggccacttcg tattggaagg tcgggtcata agatacgcag
     9241 ttcggaatca ggtcggcctg gatgtggctg tggccgtctt cgtgttgcag accttcgccg
     9301 ttcagcgtcg tacggccggc agtaccgccc agcaggaagc cgcgcgcgtg catatcgccc
     9361 gccgcccaag ccaagtcgcc gatacgttgg aaaccgaaca tggagtagta gatgtagaac
     9421 ggaatcatcg cgaagtcgct gttggcgtag ctggtcgcag ccgcaatcca gtcggccatc
     9481 gcgcccggtt cgttaatacc ttcttgcaag atttgaccgt cgacggattc tttgtagaac
     9541 atcagttggt ctttatcttg aggcgtgtat tgttggcctt tgggattcca aataccgtat
     9601 tggcggaaca taccttccat gccgaaagta cggctttcgt cgggaacgat aggtacgacg
     9661 cgtttgccga tttttttgtc tttcagcaaa gtggacagga tgcggacgaa tgccatcgtg
     9721 gtcgagaact cgcgttcacc gctggatttg agttgtgcgt cgaatgccga cagctcgggc
     9781 acttccaata cttcctgcgt cggtttgcgt tgcggcaggt agccgcccaa agcatcgcgg
     9841 cgtgcgtgca ggtatttgta ttcttccgta tcgggggcaa aagtcaggta aggcagatcg
     9901 ccgctttcga tttgttcgtc ggtaaccgga atgtcaaagc ggtcgcggaa ttgtttcagg
     9961 gacgctttgt ccattttttt ggcttggtgg gcaacgttct gaccttcgcc ggatgcgccc
    10021 ataccgtaac ctttaatggt tttcgccaag ataacggtag gtttgccgtc ggcatggttc
    10081 gctgcgcggt cgtaggcgtt gtacactttt tgaggatcgt ggccgccgcg gttcaatgcc
    10141 cagagttgct catcggtcat atcggcaacc aatgctttca gttcgggcgt attgaagaag
    10201 tgttcgcgca catacgcgcc gtctttggat ttgtaagttt ggtagtcgcc gtccaaacat
    10261 tcttccatac gttggcgcag gataccgtct ttgtctttcg ccaagaggcg gtcccaacgg
    10321 cggccccaaa tgactttgac gacattccag ccggcaccgg caaagttgcc ttccaattct
    10381 tggatgattt tgccgttgcc gcgcaccgga ccgtccaagc gttgcaggtt gcagttgatg
    10441 acgaaaatca ggttgtccaa gccttcgcgt gcagccagcg cgattgcacc ttgggattcg
    10501 ggttcgtcca tttcgccgtc gccgcagaaa caccatactt tacggccttt ggtttttgcc
    10561 aagccgcgtg attccaagta tttcaggaaa cgcgcctgat aaatcgccat gatggggccc
    10621 aagcccatgg atacggtcgg gaactgccag aagtcgggca agaggtgggg gtgtggatag
    10681 gaaggcagac cgtgtccgtc cacttcttgg cggaagttgt tcagctggtc ttcggtcaga
    10741 cggccttcga cgaatgcgcg tgcatagatg cccggggcaa cgtgaccttg gaagaagacc
    10801 aaatcgcctt cttcaccttc acctttggct ttccagaagt ggttgaaacc gacttcgtac
    10861 atggtggcgg cagattggaa agatgcgatg tgcccaccca gttccaaatc tttcttgccg
    10921 gcgcgcaata cgatggcggc ggcgttccag cgtacgaatg cgcgaatgcg gtgttcgatg
    10981 ttttggtcgc ccggaatgcc tttttcgttt tcaaccgaaa cggtattcaa atacggggtg
    11041 gtcgtgccgt ggggcatacg tacgcctttg tcgcggcagt atttgagcag gttttccaag
    11101 aggtattgcg cgcgttcgcc gccttcatat tcgaggacgg agcttaacgc gtccagccac
    11161 tcttgggttt cgatagggtc aacatcgtgt aattgggtgg acatagtatc tatcctttat
    11221 gttgagtgtt taatcggaag ggagggtgta gccaacccct ttattttcgt ttgtgaaaaa
    11281 taataagcaa aaacgaaaat ttctaaatca aaccgaaaca aaataattta agtttaatgt
    11341 tttcaaaaaa ttataaaagt cttggaagca agggaaaact tgaaaaaaaa tgttcggtct
    11401 gctccgcacg aaatcctatc agcaatcggg tctgctggca aataaccatg cttttaaaag
    11461 caggttttat actgaagttg gcgtttggta aggacaggtc tttttgattg cgaaaacatt
    11521 ctgtttaatt ttggttatat tttttcggaa ttgtgggata tcgaatcgtt atgtctctgt
    11581 cggcaatttt cctattgcag aaaatctata cacaagcttt gtggacaacc gtgttgataa
    11641 gccacggctt gtgttaaaaa agtcttacgc aacaaatgta cgcttatttt gattaaaaat
    11701 taggcaaatt taaatctata aaaatcatgt aattatgttc aatatgatgt ttctggtcat
    11761 gtttttgccg tctgttatgg ggtttgtccc gcgtttgggg cagggggtga attttggagg
    11821 ttggtgcaaa acctgctttt ttcgcgttac aatctgcacc cgatttactt ttgaatctgc
    11881 ctgtttggaa aaatactttt atgaaagcca gccaattctt tatctctact ttaaaagaag
    11941 cccctgccga agccgcgttt gccagccaca agctgatgat tcgcgccggt ctgattaaag
    12001 ccaacgcgtc cggtctttat acttggatgc cgatggggct gcgcgtgtta cgcaaagtcg
    12061 aaaacgtcgt gcgcgaggaa atggcgcgcg cgggcagcgt ggagctgctg atgccggtgg
    12121 tgcagcctgc cgaactgtgg caggaatccg gccgctggga gttttacggt aaagaactgc
    12181 tgcgcctgaa agaccgccac gaacgcgatt tctgcatggg cccgacctgc gaggaagtca
    12241 tcgccgacat cgtgcgcaaa gaaatcaaca gctacaaaca actgccgaaa aatttttacc
    12301 acatccaaac caaattccgc gacgaagtgc gcccgcgttt cggcgtgatg cgcgcgcgcg
    12361 aatttgtgat gaaagatgct tattccttcc acgccgacta cgcctcgctt caggcgacct
    12421 atgatgccat gtatgacgct tactgccgca tctttacccg tctgggtttg gcgttccgcc
    12481 ccgtcgccgc agacaccggc agcatcggcg gtacgggttc gcacgagttt caagtgttgg
    12541 cggaaagcgg cgaagatgtg attgcataca gcgacacttc cgattacgcc gccaatatcg
    12601 agttggcacc gaccttgccg cttaaaggtg aacgtgccgc cgctcaggct gtgttgacca
    12661 aagtacatac accaaacgtc aaaaccattg agtctttggt tgaattcctg aatattccgg
    12721 ttgaacaaac cctcaaatcc atcgtggttg aaggcgaaaa cgaaggcgaa ctcgtcctac
    12781 tgctgttgcg tggcgaccat gagtttaacg acatcaaggc agaaaaactg gcgggcgtaa
    12841 aatcgccact gactatggca agccctgccg cgattgttga acaattcggc gcaaacggcg
    12901 gttcgctcgg ccccgtcggc ttcacaggca aagtctatgc cgatttcgct accgaaaaag
    12961 gcgcggactg ggttatcggc gcaaacgaag acgactacca ctataccggc ttcaacttcg
    13021 gccgcgatgc cgccgagcct gaattcgtcg atttgcgtaa tgtggtcgaa ggcgacgaaa
    13081 gccctgacgg gcaaggccgt ctgaaactgg cgcgcggcat cgaagtcgga catgtcttcc
    13141 agttgcgcgg caaatatacc caagctatga acgtcagctt cctcgacaat aacggcaaat
    13201 cgcaaatcat ggaaatgggc tgctacggca tcggcatcac ccgcgtcgtt gccgccgcca
    13261 tcgagcagaa taacgacgaa aaaggcatca tttggaccaa agcgatggcg ccgtttgaag
    13321 tcgtcatcgt gccgatgaac tacaaaaaat ccgacactgt gcgtgaagcc gccgacagaa
    13381 tctatgccga attgctggcg gcaggcgcgg atgtgctgct ggacgaccgc gacgaacgcg
    13441 caggcgtatt gctgaacgat tccgagcttc tcggtatccc gcaccgcatc gtcatcggcg
    13501 accgcgcctt gaaagaaggc aatgtcgaat acgccgaacg ccgcgacaac gaagcgcagg
    13561 cagttgcaat cggagaaatt gttgcgcgtg taacagcttc attaaatgcg taagtctggt
    13621 caaatgccgt ctgaaaataa tttcagacgg catttttatt ttgggggaaa gtgaagtggc
    13681 ttcagtattt ttagaaggag aagcagtcga ggtcggcaat attttctgta tcggcaggaa
    13741 ctatgccgca catgtcgaag agttaaaaaa cgaaatacct tcagaacctg tggtgtttat
    13801 gaagccgtca ggcagcattt tgaacagcgg cggtacaatc ttgctgcccg agttcagtag
    13861 ggatgtgcag tttgagtgcg aactagtgtt gctgattggc cgggattcag acggcacggg
    13921 cgagggtgaa gatattttgg gatgcgtggc ggggtacggc gtgggtttgg atttgaccgc
    13981 gcgggacatc caatgccgtc tgaaggcgca ggggctgccc tggctgaagg caaaagggtt
    14041 caggcattct gcgtgcgtgt cggattttgc ggcggcagac agaatcggta atcaagataa
    14101 ggtgttgttt tcattaaaac aaaacggcgt attgaaacag cgcggggaga ctggtttgat
    14161 gatttacccg attcgggaaa ttttacacaa actggcggcg gattacggtt tggggaaagg
    14221 ggatttggtg tttaccggaa cgccctccgg cgtgggcgca atcggtgcgg gggacaattt
    14281 ggcattggag ctggacggct tggtgtgcgc ctctttcacg atagggtgtt aaccgcgccg
    14341 cttaaccttc acgcccgttg aaacattccg ccgggccgcc ctcgagaaaa ccgtgcagga
    14401 tggcttgcgc cgccacttgg tcgagcaccg atttgcgttt tttgcccaag acctgtgctt
    14461 ccgaaagcag gctttcggca tagacggacg acagccgttc gtcaacccaa tagacgggga
    14521 gattgaacct gccgttcagc ctgcgtccaa acttgcgcga caggtgcgtc atttcatgtt
    14581 ccgtgccgtc ggcatgcacg ggcagtccga cgacaaaata acgcggctgc cattcttgaa
    14641 ccagcttggc gattgccgcg aatttttcat cgttgctgcc gccggtaacg gtggccagcg
    14701 gatgggacag ccccaattcc gcatcgccct gcgccacgcc gatgcgcgct tcgccgaagt
    14761 cgaatgccag tgccgttcct tttggaattt tatgcatggc cggctcccga aaacagggcg
    14821 agcgggtcga tgccgagttt ggcgaatgcg gcggcgtaac ggtgttcgta ggggatgtcg
    14881 aacaggatgt gttcgtcggc gggaacggtc agccacgcat tgtcggcaag ttcgcgttcg
    14941 agctgccctt tgctccagct cgaatagcct atgctgatca aggctttgtc aaccgcgcct
    15001 tcgcgtgaaa tgttttcaag cacatcgcgg gaagaggtca gcgcgatgcc gtctgaaacg
    15061 ccgatgctgc tttgccagtt gccgatcgga gtgtggacga cgtaaccgcg ctcgacctgc
    15121 accggcccgc ccatcatcac gctgtcgtgc tgcatccgca tggggatgtt tttgccggtg
    15181 gcggaaaaaa tcatgtccat cgtaatcgga gagggtttgt tgatggcgat accgagtgcg
    15241 ccgtcttcat cgtgtttgca gatatagacg accgattgtg aaaaaaacgc gtcttccata
    15301 tcgggcatgg cgacgagaaa gtggttggac aggttcatat cagcgtttgt cgggattttt
    15361 tatcgggttg gaaataatgc aggcgccggc aagcagtttg ccggaagtat cggcttgcac
    15421 cccgtcggtt tgcggtatgc cgctgccgaa acaggaaacc gcgcttaagg aatttttcgg
    15481 cgatccttgg acgattttat cgctgtaaac caaataggcg aaggctttgc gtttggggtc
    15541 gtaataacgg acaatctgcc ggctcttgaa cgcgaagctc gcgccgcgct tgaaaacttc
    15601 tttcggtttg cgcacggcgg tttcgtcaaa agaaatcgaa gatgccgtct gaacgcacga
    15661 aaccgatgcg tcggacgcgt cctcttccaa attgaccatt tccttcaagc cgcctttttt
    15721 tgcatacgaa atataacagg caaccccttg aacgtcggga tcgtcgaatc cttccacttc
    15781 gatacggtcg tttttgccca acatgttgaa aacggtactt gcccgtccga ttttatcggt
    15841 ttcgccgctg ccgcaggcag tcggcaggac ggcggcagac agcagtagca gtctgttcat
    15901 caaaatttcc ttatcggttg aatccccgtt ttccggaagg cggcagggtt tgctttgttt
    15961 ggaggggcgc acgctcccaa atcgggcaac atacggtacg cagcttacat gtgctaccct
    16021 gtgtgtcgga acacaatatc cgttgcagca ttgaaaatat gccgcacaat ataaggctgt
    16081 ttgagccgat tgcaagtatc cgcgcaaaga aggtgcaaga ttgggcaaac ggcattttca
    16141 gacggcataa ctgacagtat aatccaaacg tccggctgct ttcgtgcagc cttcagtatt
    16201 atccaacttt tccgaaggtc gaaccatgcg ctacaaaccc cttctgcttg ccctgatgct
    16261 cgttttttcc acgcccgccg ttgccgccca cgacgcggca cacaaccgtt ccgccgaagt
    16321 gaaaaaacag gcgaagaaca aaaaagaaca gcccgaagcg gcggaaggca aaaaagaaaa
    16381 aggcaaaaat gccgcagtga aagataaaaa aacaggcggc aaagaggcgg caaaagagtt
    16441 caaaaaaacc gccaaaaacc gcaaagaagc agagaaggag gcgacatcca ggcagtctgc
    16501 gcgcaaagga cgcgaagggg ataaggaatc gaaggcggaa cacaaaaagg cacatggcaa
    16561 gcccgtgtcc ggatccaaag aaaaaaacgc aaaaacacag cctgaaaaca aacaaggcaa
    16621 aaaaggggca aaaggacagg gcaatccgcg caagggcggc aaggcggaaa aagacactgt
    16681 ttctgcaaat aaaaaagccc gttccgacaa gaacggcaaa gcagtgaaac aggacaaaaa
    16741 atacagggaa gagaaaaatg ccaaaaccga ttccgacgaa ttgaaagccg ccgttgccgc
    16801 tgccaccaat gatgtcgaaa acaaaaaagc cctgctcaaa caaagcgaag gaatgctgct
    16861 tcatgtcagc aattccctca aacagcttca ggaagagcgt atccgccaag aacgtatccg
    16921 ccaagagcgt atccgtcagg cgcgcggcaa ccttgcttcc gtcaaccgca aacagcgcga
    16981 ggcttgggac aaattccaaa aactcaatac cgagctgaac cgtttgaaaa cggaagtcgc
    17041 cgctacgaaa gcgcagattt cccgtttcgt atcggggaac tataaaaaca gccggccgaa
    17101 tgcggttgcc ctgttcctga aaaacgccga accgggtcag aaaaaccgct ttttgcgtta
    17161 tacgcgttac gtaaacgcct ccaatcggga agttgtcaag gatttggaaa aacagcagaa
    17221 ggctttggcg gtacaagagc agaaaatcaa caatgagctt gcccgtttga agaaaattca
    17281 ggcaaacgtg caatccctgc tgaaaaaaca gggtgtaacc gatgcggcgg aacagacgga
    17341 aagccgcaga cagaatgcca aaatcgccaa agatgcccga aaactgctgg aacagaaagg
    17401 gaccgagcag caactgaaca agctcttgag caatttggag aagaaaaagg ccgaacaccg
    17461 cattcaggat gcggaagcaa aaagaaaatt ggctgaagcc agactggcgg ctgccgaaaa
    17521 agccagaaaa gaaaaagaag cggcgcagca gaaggctgaa gcgcgacgtg cggaaatgtc
    17581 caacctgacc gccgaagaca ggaacatcca agcgccttcg gttatgggta tcggcagtgc
    17641 cgacggtttc agccgcatgc agggacgttt gaaaaaaccg gttgacggtg tgccgaccgg
    17701 gcttttcggg cagaaccgga gcggcggcga tgtttggaaa ggcgtgttct attccactgc
    17761 gcctgcaacg gttgaaagca ttgcgccggg aacggtaagc tatgcggacg agttggacgg
    17821 ctacggcaaa gtggtcgtga tcgatcacgg cgagaactac atcagcatct atgccggttt
    17881 gagcgaaatt tccgccggca agggttatac ggtcgcggca ggaagcaaaa tcggcacgag
    17941 cgggtcgctg ccggacgggg aagaggggct ttacctgcaa atacgttatc aaggtcaggt
    18001 gttgaaccct tcgggctgga tacgttgatc ggattgccga aacggcaggc tttatgcaaa
    18061 tcgtgccgat atggtgcgct gttctgtgaa gtttgccgtt tgccgtacct gtgttttccg
    18121 gtttttggaa atcaagagag aaagttaaag aagaatgtcg aaacctgttt ttaagaaaat
    18181 cgcactttat actttgggtg caatcagcgg cgtggccgta agtctggcgg tgcagggttt
    18241 tgccgccgag aaggacgggc gggataacga agtcctgccg gtgcaatcca tccgtacgat
    18301 ggcggaggtt tacggtcaga ttaaggcaaa ctactatcat gacaaacccg atgccgattt
    18361 gtttgaaggt gcgatgaagg gtatggtggc cggtttggat ccgcattccg aatatatgga
    18421 taaaaaaggt tatgccgaga taaaggagtc caccagcggc gaatttggcg gcttggggat
    18481 ggaaatcggg caggaagacg gttttgtcaa agtggtttcg ccgattgagg acacgcctgc
    18541 cgaacgggcg gaggtgaaaa gcggcgattt cattgtgaaa atcgataatg tttcgacgcg
    18601 cggtatgacg gtcagcgaag cggtgaaaaa aatgcggggc aagccgggta cgaagattac
    18661 tttgacgctg tcgcgcaaaa atgccgacaa gccgatagtc gtcaacctga cccgtgccat
    18721 tattaaagtg aaaagcgtcc gccatcacct gatcgaaccc gattacggct atatccgcgt
    18781 gtcgcagttc caagagcgga cggtcgaaag cgtcaatacc gccgcaaaag agctggtaaa
    18841 ggaaaataaa ggaaaaccgc tcaaggggct ggtgttggat ttgcgcgacg accccggcgg
    18901 gcttttgacc ggcgcggtcg gcgtgtcggc ggcgtttctg ccgtctgaag cggtcgtcgt
    18961 cagcaccaag ggacgcgacg gcaaagacgg catggtactg aaagccgttc ccgaggatta
    19021 tgtgtacggt atgggcggcg atcctttggc gggtattcct gccgagttga aaacgattcc
    19081 gatggcggta ttggtcaatt ccggttcggc ttccgcgtcg gagattgtcg ccggcgcatt
    19141 gcaggaccac aaacgcgcgg tcatcgtcgg tacgcagagc ttcggtaaag gttcggttca
    19201 gactttgatt cctttgtcca acggcagcgc ggtcaagttg acgaccgccc tgtattacac
    19261 gccgaacgac cgttccattc aggcacaggg gattgttccc gatgtcgaag taaaagataa
    19321 ggaacgtact tttgaaagcc gcgaggcgga cctggtcgga cacatcggca atcccttggg
    19381 cggcgaggat gtgaacagtg aaacccttgc cgtaccgctt gaaaaagatg cggataagcc
    19441 cgctgcaaaa gaaaaaggta aaaagaaaaa ggacgaggat ttgtcttcaa ggcggattcc
    19501 gaaccctgcg aaagacgatc agttgcgtaa ggctttggat ttggtcaagt cgcccgagca
    19561 gtggcagaag tctttggggc tggcggcgaa aaagccggtt tcaaataaag ataaaaaaga
    19621 taagaagtag gttttgacga tgccgtctga aagggtttca gacggcatat tatgtttgaa
    19681 aggacaggtt atgagcgatt ttttaccgga atttgaaccg tgcggc
//
LOCUS       DAXDZG010000040        19296 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXDZG010000040 DAXDZG010000000
VERSION     DAXDZG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19296
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.040"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB78_001216"
     CDS             complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB78_001216"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC1269207.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB78_001217"
     CDS             complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB78_001217"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC1269208.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRNREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2249..2431
                     /locus_tag="ACFB78_001218"
     CDS             2249..2431
                     /locus_tag="ACFB78_001218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269209.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2434..3840
                     /locus_tag="ACFB78_001219"
     CDS             2434..3840
                     /locus_tag="ACFB78_001219"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC1269210.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3903..4343
                     /locus_tag="ACFB78_001220"
     CDS             3903..4343
                     /locus_tag="ACFB78_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC1269211.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4603..5421
                     /locus_tag="ACFB78_001221"
     CDS             4603..5421
                     /locus_tag="ACFB78_001221"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1269212.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5528..6154
                     /gene="purN"
                     /locus_tag="ACFB78_001222"
     CDS             5528..6154
                     /gene="purN"
                     /locus_tag="ACFB78_001222"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC1269213.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6170..6841
                     /locus_tag="ACFB78_001223"
     CDS             6170..6841
                     /locus_tag="ACFB78_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC1269214.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            6959..7381
                     /locus_tag="ACFB78_001224"
     CDS             6959..7381
                     /locus_tag="ACFB78_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC1269215.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7904..8620
                     /locus_tag="ACFB78_001225"
     CDS             7904..8620
                     /locus_tag="ACFB78_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC1269216.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8769..9671
                     /locus_tag="ACFB78_001226"
     CDS             8769..9671
                     /locus_tag="ACFB78_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC1269217.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9714..10488
                     /locus_tag="ACFB78_001227"
                     /pseudo
     CDS             9714..10488
                     /locus_tag="ACFB78_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            10600..12288
                     /locus_tag="ACFB78_001228"
     CDS             10600..12288
                     /locus_tag="ACFB78_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC1269218.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPGAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            12380..13336
                     /gene="gshB"
                     /locus_tag="ACFB78_001229"
     CDS             12380..13336
                     /gene="gshB"
                     /locus_tag="ACFB78_001229"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC1269219.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKEFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GKVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13495..13878
                     /locus_tag="ACFB78_001230"
     CDS             13495..13878
                     /locus_tag="ACFB78_001230"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC1269220.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14010..14483
                     /locus_tag="ACFB78_001231"
     CDS             14010..14483
                     /locus_tag="ACFB78_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC1269221.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14608..15711
                     /gene="mnmA"
                     /locus_tag="ACFB78_001232"
     CDS             14608..15711
                     /gene="mnmA"
                     /locus_tag="ACFB78_001232"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC1269222.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15782..17452
                     /locus_tag="ACFB78_001233"
     CDS             15782..17452
                     /locus_tag="ACFB78_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC1269223.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPNEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17564..19198
                     /locus_tag="ACFB78_001234"
     CDS             17564..19198
                     /locus_tag="ACFB78_001234"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC1269224.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         4727 a         5175 c         5235 g         4159 t
ORIGIN      
        1 agggaacggc tatatgtctg cgggaatgtg tcaatgaaaa caaatgccgt ctgaagagag
       61 gttcggacgg catttgttca acgtttttcc tgtttgcgta tcagccaaac agcgagcaag
      121 gcgaaggcta tggtaggcag tgcgccggcg aggaagggtg gggtgccgta gagttggctg
      181 gtaaacccga agagcctgcc ggcaaggtgg aacagcaatc cgagacagat gccgccgaag
      241 agttttaagc ccatattgcc gtggcgcgtg gtttgcggcg taaaggcgaa ggcaacgagc
      301 gccatgaccc atgcggcgac ggggtaaacg agtttacgcc accatgcgat ggcgtagatt
      361 tgggtgtttt ggctgttgtt ttggaggtgg cggatgtagg tggtcagctc gccgacggac
      421 atttggtcgg gcttgacgag caatacgtcc atcaggttgc gtctgacggc aatcggccaa
      481 gtttcttcgg cggcggcgga tgtttcgatt ttgtctgtac ccatgatgct gcggcggatg
      541 tttttcaact gccagctgcc gtcgctgttc aaaacggcgg aatcggcttc cactgcctct
      601 gccaattcgt ttttatcgtt gcgcgcccaa attttgatgc ccaaaagcgt atggtcgggc
      661 aacattccgc gcacattgat aatgctgttt ttttctttca gccaaaggcc ggtatggccg
      721 gtgctgattt tgccgttaat ggcggcggct ttgatgtttt cggctttttg gctcagcgtg
      781 ggcgcaaccc attcgccgag cgcgacggcg gcaatagcaa aaataaaacc gaactgagac
      841 agaatcaaca gcagcttttt ggtgctcatg ccgctggctt tgatgacggc cagttcgctg
      901 ccggcggcaa gctggctgag agaggccagt ccgccgatga ggacggcgag gggcatgagt
      961 tcgtaggcgc gggcgggcat tttgagggcg gtgtagccca gcatttccca tatgccgtaa
     1021 ctgcctttgc cgaggttgcc ggtttcgtac aggatttcaa aaaagctgta caaagcgagg
     1081 aaggcaagga gcgcgtaaac cgccataacc gccatttggc ggatgatgta acgtgaaatc
     1141 aggttcattt tccgcctttc aatgtcagac ttttgccaac cgcctgccag aagggctggc
     1201 tgggcatact gcggacgcgc agaagtacga ttgcgatgac gaacatgatg atgtgcatag
     1261 gcagcagtcc gagccaaaaa tggattttgc cgtcttccac ggcttcaaaa agcagggtca
     1321 gcccgttttg gtaaattaaa aacaaaccga tggcaatcaa gatattgtag gtatgtccgc
     1381 tgcgcgggtt gaaataggaa agcggcacgg cgagtaggca gagcaggagg acgctgacgg
     1441 tcagcgagat gcgccacatc aattctgcct gatgttgcgg attgctgctg ccaatcagtt
     1501 gggcggtcga aatggtgcgg cggtgggaaa cggggtcgat aagtttgggc gtggtgctga
     1561 taatcaggtt gagtttttgg aaggaaacct gattgtagtc ggcgcgtccg ggcgtgccgc
     1621 tgtaacggta gccgtggcgc aattcgagcg tgcgtttgtt gtcgttcagc gagaagttac
     1681 cttctttggc gaagatgatg ttgtcgccgc cgtttttgtc ctgttcgcgc aggaacaggt
     1741 ttttcatgat gccggattcg gtgtcaaagg tttcgacgaa ataaaccctg ccgttgcgct
     1801 tgcccaagtt attgaactcg ccggcttcca ccaaagacaa ttcctgcttc tgcttcaaaa
     1861 tttcggcata ttcgcggttg cgcagttccg cccacggtat cacccaaagc tgcatgacgg
     1921 caatcaggat ggcaaacggc acggcaaact gcatgacggg gcgtatccac tgtttcaacg
     1981 ccaatccgca ggatagccag accgacattt cgctgtcgcg ccagtagcgg gtcaatacgg
     2041 tcagcgtgct gatgaatgcg gtcaacacca gcaaaagcgg ggtcataccg atgacccaga
     2101 agccgactaa ggccaacacg gcatcgatgg cgacacgccc gtcagctgcg cggccaagca
     2161 ggttgatcgc ctgcgtggac accaacaccg ccaagaggac gacgaaaatg ccgacggcgg
     2221 taaaagagag ttctttgatg aggtttcttt gataaatcat aaaatcgcgg tcaggggggc
     2281 ggtaagcggt tcggcgcagg cggattgtcc gcgcaaggtt tcagacggtg cttcaaaaaa
     2341 tggggtacaa tcgtgccagc cgtcttcaga cggtcttttg agcgaaaggc cgtctgaaaa
     2401 ctaccgacaa ccgtctcaat caggagaata aacgtggaat ttagcacaaa aaccgaaatc
     2461 ttgcaggaac agcaggcagg cgcgcagtta tttgtctgtg ccgacaaagc acccgagcac
     2521 aacaccgccg cacatgcgct cttctctgct ttggaagagg gtcagaattt ttccgacacc
     2581 aaaatcccga cggacaacgg tttgcaggca gtcgccgtcg tccgcctcga aaaaaccgac
     2641 cgcgccgcgc tgaacaaagc cgccgccgaa gccgccaaat gggcgcaaaa tcaagaaacg
     2701 gtcaatgtgg acgttcacgc ctttgatgag gcgcaagcag cagccgttgc cgaagctttt
     2761 gccatcgcgt tcggcaacgc tgcatatcgt ttcgaccgct acaaaaaaga agccaagccc
     2821 gccaaatttt cgcaagccgt gttccacagc gcacacgaag ccgccgtcaa agaagccctg
     2881 cgcgttgccg aagcacaagt ttacggacaa agcctctgcc gcgacttagg caatgccgca
     2941 ccgaacgaat gcacgcctga gttcctcgcg cgtaccgcca aagccgaagc cgagaaactg
     3001 ggcgcgcacg ccaaaatcat cgaaaaagac tacatcaaag aaaacatggg ttcgttttgg
     3061 tcggttgcca aaggcagcgt cgaagaccca tatttggttg aactgagcta tttcggtgcg
     3121 gccgacaaag aagccgcgcc tgtggtattg gtcggcaaag gcattacctt cgacaccggc
     3181 ggcatttccc tcaaacccgg cctgaacatg gacgaaatga agtttgacat gtgcggcgcg
     3241 gcaaccgtca tcagcacctt ctgcgccgcc gtcaaactgc aactgccgat caatctgatt
     3301 gccatcgtcg ccacttgtga aaacatgcct tccggcgcgg caaacaaacc gggcgatgtt
     3361 gtgaaaagta tgaaagggct gacgattgaa gtgttgaaca ccgatgccga aggccgtctg
     3421 attttgtgcg acgcgctcac ttacgccgag cagttcaaac ccaaagccgt catcgatgtc
     3481 gccaccctga ccggcgcgtg catcgtcgcc ttggggcacg atgtcagcgg cgtgatgggc
     3541 aataatcagg atttgatcga cagcctgctt gccgcttcct acaacgtgga cgacaaagcg
     3601 tggcaactgc cgctctttga aacctacaaa gaccagctca aatccaactt tgccgacatc
     3661 cccaatatcg gcacgcccgg cgcgggcacg attaccgccg caacattcct gtcctatttc
     3721 accgaaggct acccgtgggc gcaccttgac atcgcgggta cggcgtggaa atccggagcg
     3781 gaaaaaggtg cgaccggccg ccccgtaccg ctgctgatga actatctgcg gaatctttaa
     3841 ttttgccaca atgccgtctg aagcttttca gacggcatca tcctgtcaaa tgctcaaaca
     3901 atatgccgaa agttaccttt tatacccatg ttgaccaaat cccccttttt acctgtcggt
     3961 tgattgcgcg cgctatccga gacggcggca ggatactggt gtggtccgac tcgttcgggc
     4021 ggcttcagga attggacaaa atgctttggc aatacgaggc cgagagtttt attccgcacg
     4081 aaatttggga aacggaagaa gccatgccgt ctgacacatc cgtcctgctt gcctgcggcg
     4141 gcaatctgcc ccgaattccc gaaggcatgg ccgttttgaa cctgtccgac ggtttttgga
     4201 acaccgcttc ggtcttgccc gcgcgcgttt tggaaatcgt cggcaacagc ctggaagatc
     4261 tcgccgacgc gcgcgaacgc tttaccgcct accgccgaag cggttttgcc atcgaacatc
     4321 acggcatgga gggcaaggca tgaaatgccg tctgaaatgc cgcatttaca aaatttaaac
     4381 aggcttgatt ctatttgatt aaaatttcct tatcggttga accccgccac ttggacatct
     4441 gtccttcggg gcggtagaat caaaccttat tttggaaggt tcaatccctt ccaaaacagg
     4501 gcaacacaca gattgacgct ttatgtgcca tcctgtgtgt tgaaacattc aaactcggct
     4561 ataatcccgt ttttccgact ttatcgacag cgaagatcca tcatgaacac cattttcaaa
     4621 atcagcgcac tgaccctttc cgccgctttg gcgctttccg cctgcggcaa aaaagaagcc
     4681 gcccccgcat ctgcatccga acctgccgcc gcttctgccg cgcagggcga cacctcttca
     4741 atcggcggca cgatgcagca ggcaagctat gcgatgggcg tggacatcgg acgctccctg
     4801 aaacaaatga aggaacaggg cgcggaaatc gatttgaaag tctttaccga tgccatgcag
     4861 gcagtgtatg acggcaaaga aatcaaaatg accgaagagc aggcccagga agtgatgatg
     4921 aaattcctgc aggagcagca ggctaaagcc gtagaaaaac acaaggcgga tgcgaaggcc
     4981 aacaaagaaa aaggcgaagc cttcctgaag gaaaatgccg ccaaagacgg cgtgaagacc
     5041 actgcttccg gtctgcagta caaaatcacc aaacagggtg aaggcaaaca gccgacaaaa
     5101 gacgacatcg ttaccgtgga atacgaaggc cgcctgattg acggtaccgt attcgacagc
     5161 agcaaagcca acggcggccc ggccaccttc cctttgagcc aagtgattcc gggttggacc
     5221 gaaggcgtac ggcttctgaa agaaggcggc gaagccacgt tctacatccc gtccaacctt
     5281 gcctaccgcg aacagggtgc gggcgaaaaa atcggtccga acgccacttt ggtatttgac
     5341 gtgaaactgg tcaaaatcgg cgcacccgaa aacgcgcccg ccaagcagcc ggatcaagtc
     5401 gacatcaaaa aagtaaatta agtccgaatc catgcccgaa acaggttttc gggcattttt
     5461 acggcaaatg ccgtctgaag ccaccaaaca gcggttcaga cggcatccct ttcaaaagcg
     5521 caccattatg aaaaacatcg tcatcctgat ttccggacgc ggcagcaata tgcaggcaat
     5581 cgtcaatgcc gccattccca acgtccgcat tgccgccgtg ttgagcaaca gcgaaacggc
     5641 tgccggtttg caatgggcgg ccgaacgcgg catcccgacc gacagcctga atcataaaaa
     5701 ctttgaatca cgacttgcct tcgatacggc catgatggaa aaaatcgacg catatcaacc
     5761 cgatttggtg gttttggcag gttttatgcg gattctgacc cctgagttct gcgcccatta
     5821 cgaaggcagg ctgatgaaca tccatccgtc catcctgccc tcgtttaccg ggcttcatac
     5881 gcacgaacgc gctttggaag caggttgccg cgttgccggc tgtacgattc atttcgttac
     5941 ggccgagctg gattgcggcc cgattgtatc gcaagggatt gtgccgatac ttgacggcga
     6001 tacggcagac gatgttgccg cccgggtttt ggctgtcgaa cacaaactct atccgaaagc
     6061 cgttgccgat tttgccgccg gccgcctgat tatcgaggga aaccgtgtca gaaattcgga
     6121 aaacgccgat gccgcccgtt tcctgacggc gtaaaccggg cgggagcaaa tgatgaagac
     6181 ttttaaaaat atattttccg ccgccatttt gtccgccgcc ctgccgtgcg cgtatgcggc
     6241 aaggctaccc caatccgccg tgctgcacta ttccggcagc tacggcattc ccgccacgat
     6301 gacatttgaa cgcagcggca atgcttacaa aatcgtttcg acgattaaag tgccgctata
     6361 caatatccgt ttcgagtccg gcggtacggt tgtcggcaat accctgcacc ctgcctacta
     6421 taaagacata cgcaggggca aactgtatgc ggaagccaaa ttcgccgacg gcagcgtaac
     6481 ctacggcaaa gcgggcgaga gcaaaaccga gcaaagcccc aaggctatgg atttgttcac
     6541 gcttgcctgg cagttggcgg caaatgacgc gaaactcccc ccgggtctga aaatcaccaa
     6601 cggcaaaaaa ctttattccg tcggcggcct gaataaggcg ggtacgggaa aatacagcat
     6661 aggcggcgtg gaaaccgaag tcgtcaaata tcgggtgcgg cgcggcgacg atacggtaac
     6721 gtatttcttc gcaccgtccc tgaacaatat tccggcacaa atcggctata ccgacgacgg
     6781 caaaacctat acgctgaagc tcaaatcggt gcagatcaac ggacaggccg ccaaaccgta
     6841 aatcggtttt caggcggttt tttgccgttt tgtccatatt tctgtataag ccgcttgaaa
     6901 ttgtttacaa taacgccata tgcaaagcgg ttttaacgct attttcggga atgacaccat
     6961 gcaggttaca tcaaaatgga tagacggaat gtgttttgtc ggcacggcgg aaggcgggca
     7021 cagcgtcgtt atggagggat cggcggcaga aggcgcggcc aagcgcgggc ccagcccttt
     7081 ggaaatgctg ctgttggggg tggcgggctg ttcgagcatc gatgtggtga tgattgccga
     7141 aaaacagcgt cagaaagtga ctgactgccg tgcgacggtt acggcgaaac gggcggacga
     7201 tgcgccgcgc gtgtttaccg aaatccacat ccatttcaag gtaatcgggc atgatttgaa
     7261 agaatcggcc attgagcgcg ccgttcagat gtctgccgaa aaatactgtt cggcttcgat
     7321 tatgttgggc aaagcggcaa agattaccca cagttttgaa attgccgggg cggagaaata
     7381 ggcatcgggc ggcattatgg ggaaacggct gatgccgtct gaaggggaaa ggtgcggcag
     7441 gcaatccgga ggcgcataaa aaagcagata tattcggact gcacctcccg aatatatctg
     7501 cctgctgttt cctctttatt cagcctttat aatacttgga cttgtcgggg tattgtgcag
     7561 gcttgattcc ggattgtcaa caattttcgg tcaaatttta aatgccgcgt tttaaaatga
     7621 tgcccgcctg attttgcggg cgggcggaag gcgggaatcc cgttatttta agccatatat
     7681 taatttattg aattaaaata aatttgtgca atatagtgga ttaacaaaaa ccagtacggc
     7741 attacctcgc cttgccgtac tatttttgta ctgtctgcgg cttcgtcgcc ttgtactgat
     7801 ttaaatttaa tccactatac tttaatttct cctacacgat ttgaaacaca gccgtcttaa
     7861 aacatcttaa aaaatttgaa agcgtaaaaa tacggaaaac atcatgaact ttgaaaacga
     7921 cgacattatc catgcgccga ccacgtcttc cctgattctc gaagagcggc acgattcgga
     7981 gctgttccgt gtttacgccc tgattttgga cggcattacc gatcaggtgc tgctgcccgg
     8041 caaaaagctg accgaatccg aactttgccg tcagatggtg tgttcgcgca acaccgtccg
     8101 cggcgcgctg tcgcttttgg cgcacgacaa gattgtcgat ttgcaaccca acaggggcgc
     8161 gttcgtccac gttcccgatt tgaaagaaat gcaggatgtg ttcaatgcgc gcatcgaaat
     8221 ggagacgatg attttgaaca tcctcgccgg cctgccggat ttggaaacgc gcctcaagcc
     8281 gctttatgcg atgataaggt gcgaagaaga ggcctccggc aggggcgacc gcgtcggctg
     8341 gaaccgcctg tccaatgcct tccacgtcga actggcgcgc ctggtgggca acgatgtgct
     8401 gttcgacatt atgaacacgc tgtgcgcgcg gtcttccctg attgtcgccg tggcgggcgt
     8461 gcatcgcgag aaaaaacacg ccatcaatac gcatacgcat tccgaacacc gcgaaatcct
     8521 cgacctgctg ctggcgggca ggcgcaaccg cgtggtcaaa atcctgcgcc gccatttggg
     8581 caactgtatg gagcgtttgg aaaagacttt ggaagattga atgccggagc ggataagccg
     8641 cctgaacctt caggcggttt tttaatggcg aacctgatgc cgtctgaaat atggatgcgg
     8701 gtatctgcaa ttttcagacg gcaattttta agccgcacat atcatgcggc aataaaggag
     8761 ggtaggggat gagcagcctg atgacgttgt tttcggtatt ggtaccgatg tttgccggat
     8821 tttttatccg tgttcccaag ccttacctgc ccgcttcgga caaggtgctg tcggttttgg
     8881 tgtatgccgt gctgctgctg atcggcgtat cgttgtcgcg cgtggaggat ttgggttcgc
     8941 ggttgggcga tatggcgttg acggttctgt ggctgtttgt ttgtacggta ggggcgaacc
     9001 tgcttgcctt ggcagtgttg ggaaagttgt ccccgtggcg gataggggga aaagggaagg
     9061 gcgtttcggt cggcgtgtcg ggcagtgtga ggcagctcgg atgcgtactg atcggttttg
     9121 tgtccggcaa attgatgtgc gatatttgga tgccgtctga aaacgcgggt atgtactgcc
     9181 tgatgctgct ggtgttcctc atcggcgtac agctcaaaag tagcggcgta tcgttgcggc
     9241 aggttttgct taaccggcgg ggcatccggc tgtcggtttg gtttatattg tcatctcttt
     9301 caggcgggct gctgtttgcc gcatcggcag atggtgtgtc gtggacgaaa ggtttggcga
     9361 tggcttccgg cttcggttgg tattccctct cgggtttggt aatgaccgag gcttacgggg
     9421 cggtatgggg cagcatcatg ctgctgaacg atttggcacg agagctgttt gcactggcat
     9481 ttattccgct gctgatgaag cgttttccgg atgcggcggt gggggtcggc ggcgcgacca
     9541 gtatggattt cacattgccc gtgattcagg gtgcgggcgg tttggaagtc gtgccggtag
     9601 cggtcagctt cggcgtggtg gtcaatatcg ccgccccgtt tctgatggtg gtgttttcca
     9661 cgctgggctg aacgcggtaa aatcggcatc ccgatgcaag gaagcagaaa acgatgaaac
     9721 cgaaaatcca aaggcatgga gagattttaa gccttgtccg ccggcatcag tttatgtcgg
     9781 tggacgagct tgccgccgca ttggacgtta ccccgcagac gatacgttgc gacatccgcg
     9841 agttggagga gggcggcagc ctgaaacgcc atcacggcgg cgcatcttcg ggcggaaact
     9901 tgccggaggg tctgcccgcc gaccgccaaa cccggtgtca aaacgaaaaa aacgccattg
     9961 cccggctgat tgcggaacac atccccgacg gttcgtcgct gttcgtcagt atcggtacga
    10021 ccatggaagc cgcggcatca gagctggtga agcggcgcag cagcctgcgg attattacca
    10081 acaatatcca cgtcgcctcc gtcgtttcgg cacgtacgga ttacacggtc atcatcacat
    10141 ccggcgtcgt ccgccctttg gacggcggta ttaccggcgt ggcgaccgtc gactttatca
    10201 accagttcaa agtcgattat gccgtgatga gcacgcacgg cgtggaaagc gacggttcgc
    10261 ttttggatta cgattacaag gaagtcagcg tcatgcaggc gatgattgcc aacgcgcgcg
    10321 tccgttttct cggcgtggat cacagcaaat tccgcagcaa cgcgctggtc aggctcggcg
    10381 gcattaccgg cgtttgacaa agtatttacc gacaggttgc ctgataccgc gatgcagaag
    10441 atgctgaaag aggcgggggt ggaatgcctg attgccgatg ccgtctgaac gctatgtcaa
    10501 agcgcgcaag tcgggtacaa taaacacatc atcaaaccgc ttcagacggc atacggaacc
    10561 tccccaatgc cgtctgaagc catctgttta aagaaaacca tgctcaataa agaccaattc
    10621 gcggacaacc atttcatccg caccatcatc gaagacgacc tcaaaagcgg caaacacgaa
    10681 gccgtccaaa cccgttttcc gcccgaaccc aacggctatt tgcatatcgg acacgccaaa
    10741 tccatctgcc tgaacttcgg tttggcgtat atttacgacg gtttgtgcaa cctgcgtttc
    10801 gacgacacca acccggaaaa agaaaacgac gaatacgtca acgccatcaa agaagacgtc
    10861 gagtggctcg gtttccattg ggcgggcgaa ccgcgtttcg cttccgacta tttcgaccgg
    10921 ctttatgact acgccgtcgg tttaatcaaa gacggcaaag cgtatgtcga tgatttgacg
    10981 cccgaagaaa tgcgagaata ccgcggtacg ctgaccgaag cgggtaaaaa cagcccttac
    11041 cgcgaccgca gtatcgaaga aaacctcgac ctgttcacgc gtatgaaaaa cggcgagttc
    11101 cccgacggca gcaaaaccct gcgcctgaaa atcgacatgg cggcaggcaa catcaatatg
    11161 cgcgatcccg tcatctaccg catccgccgc gcccatcacc acaacaccgg tgacaaatgg
    11221 tgcatctacc cgatgtacga ttacacgcat tgcatttccg atgccatcga aggcatcacg
    11281 cattccttgt gcacgctcga atttgaagcg caccgtccgc tttacgactg ggtgttggac
    11341 aacatccccg cgccgcacgc cacccgtccg cgccaatacg agttttcccg tttggagctt
    11401 ttgtacacca ttacctccaa acggaaattg aatcagttgg ttgtggaaaa acacgtttcc
    11461 ggctgggatg atccgcgtat gccgaccatt tccggtatgc gccgccgcgg ctacacgccc
    11521 gaaggggtgc gcctgtttgc caaacgcgcc ggtatttcca aatctgaaaa catcgtcgac
    11581 atgagcgtgt tggagggtgc gattcgcgaa gagctggaaa actccgcccc gcgcctgatg
    11641 gcggttttga acccgctcaa agtgaccctg accaactttc aagccggcaa aacccaaagc
    11701 cgccgtgccg cgttccatcc gaaccacgaa gaaatgggcg atcgcgaagt acctgtttca
    11761 caaaccatct acatcgaagc cgacgacttt gccgaaaatc cgcccaaagg cttcaagcgt
    11821 ctgattcccg gcggcgaagt acgcttgcgc cacggctatg tcatcaagtg cggtgaagtc
    11881 gtcaaagacg aagcaggcaa tgtggttgaa ctcaaatgca gcatcgacca cgacaccttg
    11941 ggcaaaaatc cagaaggccg caaagttaaa ggcgtgattc actgggtttc cgccgaacac
    12001 gccgccgaaa tcaaagtccg tctgtacgac cgcctcttta ccgtcgagcg tcccggtgcc
    12061 gtgcgcggcg aagacggcga atacctgccg tttaccgatt tcctcaatcc ggaatccgtt
    12121 aaggaaatca ctgcttacgc cgaacctgcc gcgaaagatt tgccggcgga aagccgttgg
    12181 cagttcgagc gcatcggcta ttttgtgacc gaccgccaag accacggcaa agacacgccg
    12241 gtgtttaacc gcacggtgac gttgaaagat tcttggcagc ctaagtaaac cccatccttg
    12301 ccgtctgaat attgttcggg cggcatttct cctttacccg cgaaatgcgg cacattcggc
    12361 acaccggaaa ggaaatatga tgaaagtcct ctttatcgcc gacccgatgg caagtttcaa
    12421 aacctacaaa gacaccacct acgcgatgat gcgggaaatg gcaaaacgcg gctggcggct
    12481 gtttcatacc ttgagcgggg aattgtctgt aaacggcggt ttggtaacgg cacaggcatc
    12541 ggcatttgaa ttttcgggtg caaaaaacga tgatgaccat gaatggttta aagcggcgga
    12601 caaagttcag acggcattaa aagaatttga tgccgtgatt atgcgtaccg atccgccgtt
    12661 cgatatgcaa tacctttact ccacccaatt actgacgctg gcggaacagc agggcgcgaa
    12721 agtgttcaac agcggacggg cgatgcgcga ctttaacgaa aaactggcga ttttgaattt
    12781 cagccgcttt accgcgccca cgctggtaac gacccgttcc gccgatgtcc gcgcattttt
    12841 gaaagaacac ggcgacatca tcgtcaaacc gctcgacggc atgggcggca tgggcatctt
    12901 ccgcctgacc gaaaaagacc ccaacatcgg cagcatcctc gaaaccctga tgcggtttga
    12961 ttcccgcacc attatggcgc aacgctacat tcccgaaatc gtacacggtg acaaacgcat
    13021 cttgattatc ggcggcaaag tcgtccccta tgctttggcg cgtatcccgc aaaacggcga
    13081 aacacgcggc aatctggcgg caggcgggcg cggtgtggcg caggaattgg acggacgcga
    13141 ccgggaaatt gcagagactc tggctcccga gcttaaacgg agcggcatcc tgctggccgg
    13201 tttggacgtt atcggcagca acctgaccga agtcaacgta accagcccga ccggattcca
    13261 agaaattatg aaacaaaaaa gtttcgacgt ggcggcaatg tttgccgatg ccgttgccgc
    13321 gtggtcggta cgttaaaccg atgccgtctg aaaggctttt gcttcgtaac cgcttggttt
    13381 gttcgggcag gcagggtttt ccctcggccg gcagggatgc gcttaatgcc gtacggacac
    13441 ccctccgccc cgttttcaga cggcatatat tgaggacatt ttgaaaggat accgatggaa
    13501 ccttcctcct acgcggcaga aaaaaaaggc aaaagcggca tcaggcgcgt catcaacgca
    13561 ttcggctatt cgatagacgg catcgccgcc gcctaccgtt acgaagcggc attccgtcag
    13621 gttttgtggc tgaacgcgct gctggtgtgc gcggcatttt tttgggtttc cgaaaccgcc
    13681 gtccgcctgc cgttgattat cgcgtctttt gtgtcggtca ttgtcgaact gttcaacacc
    13741 gccgtcgaag ccgccgtcga tcatacttcg accgaaaaac acgagctggc caaacgcgcc
    13801 aaagatgcag gttctgccgc acaactggtt gccatgctga tgttggcggc ggtttggctg
    13861 tccgccctgt tcgggtaaaa cgcttgcagc aggatttgta atcctttagg attagtattt
    13921 attattcact taatctacat caaatttccg agcagtattt gtatgtaaga ttaagcacat
    13981 tccccgtctg atattaaagg agcaggaaga tgaaaaaatt attggcagcc gtgatgatgg
    14041 caggtttggc aggcgcggtt tccgccgccg gagtccatgt cgaggacggc tgggcgcgca
    14101 ccactgtcga aggtatgaaa atgggcggcg cgttcatgaa aatccacaac gacgaagcca
    14161 aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc
    14221 acatcaacga caacggcgtg atgcgtatgc gcgaagtcaa aggcggcgtg cctttggagg
    14281 cgaaatccgt taccgaactc aaacccggca gctatcacgt gatgtttatg ggtttgaaaa
    14341 aacaactgaa agagggcgac aagattcccg ttaccctgaa atttaaaaac gccaaagcgc
    14401 aaaccgtcca actggaagtc aaaaccgcgc cgatgccggc aatgaaccac ggtcatcacc
    14461 acggcgaagc gcatcagcac taatctgctg gaaatatttg aaatgccgtc tgaaaaagcc
    14521 cgggctttca gacggcattt ttatgcccgc ctttaaaatg tgttaaaatc cgcctttaaa
    14581 aaccgccgtt tccaagccat cctgcgtatg aatacgacat caaacacttc caatatcatc
    14641 gtcgggcttt ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt
    14701 tatcaagtgc gcggtgtgtt catgcagaac tgggaaaacg acgacaacga cgaatattgc
    14761 agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattgtcgg catcgacatc
    14821 gacatcgtta atttcgccgc gcaatataaa gacaaagttt ttgcttattt tcttcaggaa
    14881 tacagtgcgg ggcgcacgcc gaatccggat gtgttgtgca atgccgaaat caaattcaaa
    14941 tgctttttgg actacgccgt agggcagggc gcggatacca ttgccaccgg acactatgcg
    15001 cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac
    15061 caaagctatt ttctctaccg cctcaagcct ttccaactcg aacgcgcgat ttttccgttg
    15121 ggcggtttgg aaaaacccga agtgcgccgc cttgccgccg aatttaattt gccgactgcc
    15181 gctaaaaaag acagtaccgg catctgtttc atcggcgagc gtccgttccg cgagtttctg
    15241 cagaaatact tgccgaccga caacggcaaa atggtgacgc ccgaagggaa aaccatcggc
    15301 gaacacgtcg ggctgatgtt ttacacattg ggtcagcgca aaggattggg catcggcggc
    15361 gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact catcgtcgta
    15421 caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc
    15481 acgctgcccg aacgtccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg
    15541 gacgcgcctt gcgaattgtg ctatttggat gatgaaaccg ccgagctggt gtttgacgaa
    15601 ccgcaatggg cggttacgcc gggtcagtcc gccgtgctgt acgacgtcga catctgtttg
    15661 ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata aaggtaatgc
    15721 cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa gaccgatacc
    15781 tatggaaaaa atctggttag aaagctacga gaagggcgtc agtgccgaaa tcgacatcac
    15841 gcaatacaat tccgtcagcg acgtattccg ccaaagcgtg gaaaaatttg cccgtctgcc
    15901 cgcttttcaa aatatgggca aaacgctcac ttatgccgaa accggcaaac tggcgaccga
    15961 tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg ttgccattat
    16021 gatgccgaac gtattgcaat atccgattgc ccttttcggt attttgcagg caggtttggt
    16081 ggcggtgaac accaatccgc tctatacgcc gcgcgagttg gagcatcagc tgaaagacag
    16141 cggtgcgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc tggtgctgcc
    16201 gcgcacgcag atcaaacacg tcatcgtcgc ctccgtcggc gaaatgttcg ggctgcttaa
    16261 aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg aataccgtat
    16321 tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg ttttccaacc
    16381 tgtcgcatta aaccgcgaag ataccgcatt gttgcaatac acgggcggca cgacaggcgt
    16441 tgccagaggc gcggtgctga gccacggcaa catctgcgcc aatatgcttc aggcaaaaga
    16501 atggattaaa aaccaattgc gcgagggaaa agaaaccgtt atcgccgccc tgccgctgta
    16561 ccacatcttc gccctgacag tgaatctgat gatttttgcc aatgccggct cgaaaattgt
    16621 cctgattgcc aacccgcgcg atatgaaagg ctttatcggc gaactgaaaa agcagcgggt
    16681 taacgtattt atcggcgtga acacgctgtt taacgcgatg gtcaaccggc ccgatttcgc
    16741 cgaagtcgat ttttcaggat tgcggctgac tttgggcggc ggtatggcga cccaaaaagc
    16801 cgttgccgaa aaatggaaaa aaatcaccgg cacgcccatc gtcgaagcct acggtttgac
    16861 cgaagccagc cccggcgtgt gctgcaaccc cttaaacatc gaatcataca gcggcagcat
    16921 cggtttgccc gtcccgtcca ccgaagtcga actgcgcgac gcaaacggca aagaagtccc
    16981 cgttgggcag ccgggcgaat tgtgggtaaa aggcccgcaa gtgatgcaag gctactggaa
    17041 ccgccccgaa gaaaccgcca aagccataga cgcgtgcggc tttttggaaa ccggcgatat
    17101 tgccgtgatg gacgaaaaag gccgtctgaa gctggtcgat cgcaaaaaag acctcgtcgt
    17161 cgtttccgga ttcaatgttt atccgaacga aatcgaggaa ttcatcgcgc accacgaaaa
    17221 agttatggaa gttgcgtgta tcggcgttcc gaacgaaaaa accggcgagg cactcaaagt
    17281 gttcgtcgtc aaaaaagacc cgtctttgac caaagaagaa ctcaccgctt tctgccgtac
    17341 cggtctgacc gcatacaaag tgccgaaaga catcgaattc cgcgacgagt tgcccaagtc
    17401 caatgtcggc aaaatcctgc gccgcgagtt gcgccaaagt gccgggaaat aaagaaaaga
    17461 taccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc cagaaccgcg
    17521 cgcttcacgt taaaatcacg cattccaaca tgggtattcc atcatgacca aattcatttt
    17581 cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt ctattgccgc
    17641 catcctcgaa tcgcgcggct tgaacgttac catgctcaag cttgatcctt atatcaacgt
    17701 cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg acgacggcgc
    17761 ggaaaccgac ctcgacttgg gacactacga acgtttcatc gattccacga tgacccgccg
    17821 caacagcttc agcacgggtc aggtgtacga aaacgttatc gccaaagaac gccgcggcga
    17881 ctatctcggc ggtacggttc aagtcatccc gcacattacc gacgaaatca aacgacgcat
    17941 ccacgaaggc gcggcgggtt acgatgtggc gattgtcgaa atcggcggta cggtcggcga
    18001 catcgaatcg ctgccgtttt tggaagccat ccgccagatg cgaagccagt tgggacgcaa
    18061 caacaccctg ttcgcccact tgagctacgt tccctacatc gccgccgcag gcgaaatcaa
    18121 aaccaagccg acccagcaca ccgtgaaaga aatgttgagc atcggcttgc aacccgacat
    18181 cctgatttgc cgtatggaca ggaaaatgcc ggcagacgaa cgccgcaaaa tcgccttgtt
    18241 ctgcaacgtg gaagagcgcg cgattgtcgg cagctacgat gtggacagca tctacgaatg
    18301 ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt tgcagcttaa
    18361 cgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccgtca aaaacccgaa
    18421 acacaccgtc aaaatcgcga tggtcggcaa atacgttgat ttgaccgaat cctacaaatc
    18481 attgattgaa gccttgaaac acgcgggcat ccataccgaa accgatgtgc agattacctt
    18541 cgttgacagc gaaagcatcg agaaaaacaa gggcgacgtt tccgtactca aagatatgga
    18601 tgccatcctc gttcccggcg gcttcggttc gcgcggcgtg gaaggcaaaa tcgccgccgt
    18661 gcgctacgcc cgtgaaaaca acgtgccata cttgggcatc tgcctcggta tgcagattgc
    18721 gctgattgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca ctgagtttga
    18781 cttgaaatgc gctgcccccg tcgtcgccct gattgacgaa tggcaaaccg ccgacggcag
    18841 cgtcgaaacc cgtgacgaat ccgccgattt gggcggcacg atgcgtttgg gcgcgcaaga
    18901 agtcgaattg aaagcaggca gcctcgccgt caaaatctac ggcagcggac acattcgcga
    18961 acgccaccgc caccgctacg aagtcaacaa caactacgtt tccgcgctgg aacaggcagg
    19021 tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca tcgaactgcc
    19081 gaaccatcct tggttcttcg cctgccagtt ccatcccgag ttcacgtcca acccgcgcaa
    19141 agggcatcct ttgttcaccg cgtttgtcaa agccgcgttg aacaataaaa aagcctgata
    19201 aagcgttact tgatgatcaa aatgccgtct gaaagcctga caaagacttt cagacggcat
    19261 tttcgcaaat cggggatcat aaaagccatc aattta
//
LOCUS       DAXDZG010000041        18856 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXDZG010000041 DAXDZG010000000
VERSION     DAXDZG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18856
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.041"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            50..1936
                     /gene="mafB"
                     /locus_tag="ACFB78_001235"
     CDS             50..1936
                     /gene="mafB"
                     /locus_tag="ACFB78_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC1269225.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            1967..2386
                     /locus_tag="ACFB78_001236"
     CDS             1967..2386
                     /locus_tag="ACFB78_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269226.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            2423..2668
                     /locus_tag="ACFB78_001237"
                     /pseudo
     CDS             2423..2668
                     /locus_tag="ACFB78_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            2665..2907
                     /gene="imm31"
                     /locus_tag="ACFB78_001238"
     CDS             2665..2907
                     /gene="imm31"
                     /locus_tag="ACFB78_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC1269227.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2985..3230
                     /locus_tag="ACFB78_001239"
     CDS             2985..3230
                     /locus_tag="ACFB78_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1269228.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEGLHHDTGHIGWEAMNKGR"
     gene            3233..3623
                     /locus_tag="ACFB78_001240"
                     /pseudo
     CDS             3233..3623
                     /locus_tag="ACFB78_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3663..5120)
                     /locus_tag="ACFB78_001241"
     CDS             complement(3663..5120)
                     /locus_tag="ACFB78_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC1269229.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(5135..5965)
                     /locus_tag="ACFB78_001242"
     CDS             complement(5135..5965)
                     /locus_tag="ACFB78_001242"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC1269230.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPTYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(6042..6431)
                     /locus_tag="ACFB78_001243"
     CDS             complement(6042..6431)
                     /locus_tag="ACFB78_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC1269231.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(6578..7105)
                     /gene="nspA"
                     /locus_tag="ACFB78_001244"
     CDS             complement(6578..7105)
                     /gene="nspA"
                     /locus_tag="ACFB78_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC1269232.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVK
                     NVRSGELSAGVRVKF"
     gene            complement(7548..8723)
                     /gene="hemW"
                     /locus_tag="ACFB78_001245"
     CDS             complement(7548..8723)
                     /gene="hemW"
                     /locus_tag="ACFB78_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC1269233.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTVEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGLIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(8780..11251)
                     /gene="ligA"
                     /locus_tag="ACFB78_001246"
     CDS             complement(8780..11251)
                     /gene="ligA"
                     /locus_tag="ACFB78_001246"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC1269234.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     ANIPLRLHGENTPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(11385..12671)
                     /locus_tag="ACFB78_001247"
     CDS             complement(11385..12671)
                     /locus_tag="ACFB78_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC1269235.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(12865..13437)
                     /gene="ampD"
                     /locus_tag="ACFB78_001248"
     CDS             complement(12865..13437)
                     /gene="ampD"
                     /locus_tag="ACFB78_001248"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC1269236.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            13521..14516
                     /gene="mltG"
                     /locus_tag="ACFB78_001249"
     CDS             13521..14516
                     /gene="mltG"
                     /locus_tag="ACFB78_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC1269237.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            14576..15196
                     /gene="tmk"
                     /locus_tag="ACFB78_001250"
     CDS             14576..15196
                     /gene="tmk"
                     /locus_tag="ACFB78_001250"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC1269238.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            15418..16698
                     /locus_tag="ACFB78_001251"
     CDS             15418..16698
                     /locus_tag="ACFB78_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFC1269239.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            17100..18131
                     /gene="lpxK"
                     /locus_tag="ACFB78_001252"
     CDS             17100..18131
                     /gene="lpxK"
                     /locus_tag="ACFB78_001252"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC1269240.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            18337..18856
                     /locus_tag="ACFB78_001253"
                     /pseudo
     CDS             18337..18856
                     /locus_tag="ACFB78_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
BASE COUNT         4327 a         4486 c         5609 g         4434 t
ORIGIN      
        1 aatttaatcc actatagttt cattccgtac catcttaagg aacatcaaat tgggcatttc
       61 ccgcaaaata tcccttattc tgtccatact ggcagtgtgc ctgccgatgc atgcacacgc
      121 ctcagatttg gcaaacgatc cctttatccg gcaggttctc gaccgtcagc atttcgaacc
      181 cgacgggaaa taccacctat tcggcagcag gggggagctt gccgagcgca gcggccatat
      241 cggattggga aacatacaaa gccatcagtt gggcaacctg atgattcaac aggcggccat
      301 taaaggaaat atcggctaca ttgtccgctt ttccgatcac gggcacgaag tccattcccc
      361 cttcgacaac catgcctcac attccgattc tgacgaagcc ggtagtcccg ttgacggatt
      421 cagcctttac cgcatccatt gggacggata cgaacaccat cccgccgacg gctatgacgg
      481 gccacagggc ggcggctatc ccgctcccaa aggcgcgagg gatatataca gctacgacat
      541 aaaaggcgtt gcccaaaata tccgcctcaa cctgaccgac aaccgcagca ccggacaacg
      601 gcttgccgac cgtttccaca atgccggcgc tatgctgacg caaggagtag gcgacggatt
      661 caaacgcgcc acccgataca gccccgagct ggacagatcg ggcaatgccg ccgaagcctt
      721 caacggcact gcagatatcg tcaaaaacat catcggcgcg gcaggagaaa ttgtcggcgc
      781 aggcgatgcc gtgcagggta taagcgaagg ctcaaacatt gctgtcatgc acggcttggg
      841 tctgctttcc accgaaaaca agatggcgcg catcaacgat ttggcagata tggcgcaact
      901 caaagactat gccgcagcag ccatccgcga ttgggcagtc caaaacccca atgccgcaca
      961 aggcatagaa gccgtcagca atatctttat ggcagccatc cccatcaaag ggattggagc
     1021 tgtccgggga aaatacggct tgggcggcat cacggcacat cctgtcaagc ggtcgcagat
     1081 gggcgcgatc gcattgccga aagggaaatc cgccgtcagc gacaattttg ccgatgcggc
     1141 atacgccaaa tacccgtccc cttaccattc ccgaaatatc cgttcaaact tggagcagcg
     1201 ttacggcaaa gaaaacatca cctcctcaac cgtgccgccg tcaaacggca aaaatgtcaa
     1261 actggcagac caacgccacc cgaagacagg cgtaccgttt gacggtaaag ggtttccgaa
     1321 ttttgagaag cacgtgaaat atgatacgaa gctcgatatt caagaattat cggggggcgg
     1381 tatacctaag gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg ataggaagct
     1441 taataaattg acaactcgtg agcaggtgga gaaaaatgtt caggaaacga gaagaaggag
     1501 tcagagtagt cagtttaaag cccatgcgca acgagaatgg gaaaataaaa cagggttaga
     1561 ttttaatcat tttataggtg gtgatatcaa taagaaaggc acagtaacag gagggcatag
     1621 tctaacccgt ggtgatgtac gggtgataca acaaacctcg gcacctgata aacatggggt
     1681 ttatcaagcg acagtggaaa ttaaaaagcc tgatggaagt tgggaggtga aaacgaaaaa
     1741 aggtgggaaa gtgatgacca agcacaccat gttcccaaaa gattgggatg aggctagaat
     1801 tagggctgaa gttacttcgg cttgggaaag tagaataatg cttaaggata ataaatggca
     1861 gggtacaagt aaatcgggta ttaaaataga aggatttacc gaacctaata gaacagcata
     1921 tcccatttat gaatagtaat atttatgaaa aattaggaga ttaatgatga aaagaattaa
     1981 gtgcttttgt gataaatttc catcaggaga tacatttaga atgtgtatca ttctggatga
     2041 ctatgataat agggttgatt attatgtagg aatatatgat tacattacgt ctaccttaat
     2101 gagcgatatt tactatcgat ccacgattga tgagcatttc aagattatag aattaataga
     2161 aaataatcca aatgaaattt atgatgatgg cggtggtcaa caattttgcc tagaatttca
     2221 tcatgataag gtcatttttt atcacaatga atttgatgaa gaagatggtt atccagtatt
     2281 aagctgttcg ctgcatactt ttaaaactgc tttaattgct tggaatgctt ttttgcaatt
     2341 gcctaaaagt attcattcgg tggtggagac tgtgattgag gaataagcat aattagctta
     2401 atgaatagaa tcagcgatat agaaaggtag aatcagatat atcccaccta aaaattactc
     2461 tccttcagca ccgctaccaa aaggacctaa taatggatat ttggataaat ttggtaatga
     2521 atggactaaa ggtccatcaa gaactaaagg tcaagaattt gaatgggatg ttcaattgtc
     2581 taaaacagga agagagcaac ttggatgggc tagtagggat ggtaagcatt taaatatatc
     2641 aattgatgga aagattacac acaaatgact aatttaaaat tagatttcta ctctgaagtt
     2701 attataaaag attcttgccc taatgatttg ttagaaaatg gtgagactat taaaggaaaa
     2761 aaaggagtgg tattaggtat aagcgaagaa gatggtataa tctatggcta tactatttta
     2821 ctttttgata taaaatattg tatatatata gataagaaat atattatacc gactggaaaa
     2881 aaattctctc gcgatgattt ttattagcta atttaagtaa tttaagcaag ttttttagat
     2941 actgaaactg gtaattatgt ttatgtaagc ataaggagtt cagattgaag gattctacag
     3001 ggcaaatgag gctggcaacc aaggatttgg cggaagccat taaacgagga gaagtacgta
     3061 gttctgcttt tacaacaaag caactaaagg caatcgaaaa aggagaatct aaaataccgg
     3121 attacacttg gcatcatcat caagacactg gaaggatgca attgattaat gaaggcttgc
     3181 atcatgatac cggccatatt ggttgggaag caatgaacaa aggaaggtaa ctatgtggaa
     3241 aatcataaaa gaggatagtg atgatttaga atttgcaatt aaatgcttat tctctcagtc
     3301 tattgattta aatgaattca agttatggat tgaacaagta atacgcgata tgcccatcga
     3361 ggacatccct ttttatattt ttgacttggc ggatttcaat gggggaattg gagacattga
     3421 caatattgta ggttttgttt cgagttacag cctatcaaaa tcgaaaaaaa atgccttgac
     3481 aggcattgcc ttcttaaggg ggatagatgt ctatagaccc tcccgtttca aaagaaaaag
     3541 cattaaaagc cttagagaaa tatcctgaaa tttatcataa atttaaatgg ttctttcctt
     3601 ttgtagagct tccgcttctt taaaggacaa tatgccgtct gaaaagtttt cagacggcat
     3661 ttttatttct tccagtaggc gggggtgaag aggatgaaga cggtgaagat ttccagcctg
     3721 cccaagagca ttgcggtaac gcagatccat ttctgcatca cgtccaaacc ggcgtaattg
     3781 ccggcgggcc cgacttcgcc cagtccgggg ccggcgttgg taatgcaggc gatgacggcg
     3841 gtaaaggcgg tggtaaattc cataccgctc gccatcagca ggaagctgaa gaggacgacg
     3901 gtcataaagt agatgaagat gaaggacata acggtcagcg cgaggcggtc gggtatggct
     3961 ttgccgctga ttttgacggt gcggacggct ttggggtgca gcagcaccat catttcgcgc
     4021 aggctgaatt tgaacaggac gagggcgcgt atggttttga tgccgccgcc ggtcgagccg
     4081 gagctggcga ggatgttggc gaggaaaaac atccacaggg aaatcaggag cggccattgt
     4141 gcgaagtcgg tgttggacag cccgtttgcc aatccgatgg agacgaagtt gaaggcggtg
     4201 tagcgcaggg attcgccgaa gccggcgtaa tggccggtgt gccacaggta cagggcggcg
     4261 gcaaggatgc tgccggagag cagcagcagc atcgtccggc attcttcgtc tttccaatag
     4321 gttttgaggc tgcggctgtt gagggcggcg aaatggctgg caaaattgat gccgccgaaa
     4381 atagtgaaaa cgatgatgac tgcttcgatg aggggggagt tgtaataacc gatgctggca
     4441 tcgtgggtgg aaaacccgcc cagcgagagg gttgccagcg cgtgacagac ggcatcgaac
     4501 caacccatcc cggcaaaatg caggcaggct gccgcgagga tggtgatcag ggtgtagccg
     4561 aaccagagtt ttttcgccac ttgggaaatg cgcggcgaca ttttgctttc tttgtcaatg
     4621 ccggggattt cggctttgaa taactgcgtg ccgcctacgc cgagcatagg caggatggcg
     4681 acggcaagga cgatgatgcc catcccgccc agccagttga gcatatgccg ccaaaagttg
     4741 acggaggggg cgagcccgtc gacgtggggg atgacggtcg cgccggtggt ggtcagtccc
     4801 gacatcgatt caaaaaatgc gtcggtaaag cccatattcg ggaaatacag gtacatcggc
     4861 atcgcagcca tagcggcaaa cgccagccac aacatcagga cgagggtgaa gccgtcgcgc
     4921 gggcgcagtt cgcgcctgaa ccggagggtg gcgagccgga cgatgcacga gccggaaagg
     4981 gtaacggtcg cggtggtggc gaatgcggtg tacgcgccgt ccgaaaaggc gtaggaaagg
     5041 gcggcgggta tcagcaggat aaaggaaaac agcataccca ggcgggagag gacgtgggcg
     5101 atgggcagga ttttgtgcat agtggggcgg tccgttattt tgcgaagctt ttccagtcta
     5161 tgccgccggc ggcttggact tgggtaattt cttccgtttc gaggttgacg gcggtcagct
     5221 gcccgcccca cagcgcgccg gtgtccagcg agatgacgtt gtcggcattc gtgtagccca
     5281 gcgaggacca gtgtccgaag atgatggtat ggtcgaggtt ttgccgatcg ggggctttga
     5341 accacgggcg caggtaagtc ggcatttttt tcactgtgga tttgtagtcg aaatccagtt
     5401 cgtttttaaa ggtcagggcg cgcatccgcg tgaaggcgtt gacgatgaag cgcaggcggg
     5461 catagccttc caaaccttcg tcccatgcgg ccggtttgtt gccgtacatt ttggagaaga
     5521 atttgacgta ttttttgccg cgcagttcgg cttcggcttc tccggcgagc gattcggctt
     5581 tggctatgcg ccattgcggc aggatgccgg cgtgtatcat cacgcggccg ccctcgcgta
     5641 tcaaaagcgg ttgcgcgcgc agccagtcga gcatttttcc gccgtcgggg tgtttgagta
     5701 tgggttcgat tgtgtcgctg cgtttgagcg cgccttcgcc gcagccgacg gcgagcaggt
     5761 acaggtcgtg gttgccgagg acgatttgca cgctgttttc gtgccggatg cagaattgca
     5821 gcgtttcgag ggatttcggg ccgcggttga cgatgtcgcc cgtcagccag agggtgtccg
     5881 tgccgtggtt gaagccgatt ttgccgagca gcgcggtcag ttcgtcgaaa cagccttgga
     5941 tgtcgccgat tgcgtaatgt gccattgcgg gtgttgtgaa gtgggaaagt gttgcggttc
     6001 ggacggcacg gttggaaatc ttatgccgtc cgaacgtgga attatgcgtt caaaacgagg
     6061 acggcttccg cttcgacctg cacgcctttg ggcagcgagg caacgccgac ggcggcgcgg
     6121 gcggggaacg gctcggtgat aaattccgcc atcacttcgt tgaagacggc aaaattgccc
     6181 aagtcggtca ggtaggcgtt gagtttgacg atgtcgtcca gcgaaccgcc tgccgcttcg
     6241 gcgacggcct gcaggttttg gaacacttgg cgcgcttcgg tgtggaaatc gccgttgccg
     6301 accacagtca tcgtggcggg atcgagcgga atctgaccgc tcatgtaaac ggtgccgccg
     6361 gcgcgcacgg cctggctgta tgcgccgatg gcggcggggg ctttgtcggt atggatgacg
     6421 gttttggaca tttcggattc ctcaaaaaat agggcggcag aagccgcagc gttcgggatt
     6481 atcgtacaaa accgccggct tgtgtagttt taggcggtat atgtgccgga aacaaaaaac
     6541 cgccgaaggc tcggcggttt gcggaataac gcgtatatca gaatttgacg cgcacgccgg
     6601 cggacagttc gccggaacgg acgtttttga cagtgttgac tttgccgatg tagttgtagc
     6661 ggtagccggc atccagatcg acattgggtg taacggcata gcttacgccc gccaatacgc
     6721 cgaggccggc ggaggttttg ctgaagctgt cgctgccgcc caagtgggcg gaagcgcggt
     6781 tgaggctcaa gcgcgcgccg aaatacggtt tgacgggcga ttgggtgtcg aagtcgtaaa
     6841 tgacggacgc gccgatgctg taaagtttga aatcggtgga tggggcttgt ttatagtttt
     6901 tgtagcgcgt gtaatcgacg gcgaagcgga ggtcgttgat gcggtagcct gcggagatgc
     6961 gcgggctgaa gcctttggca gaacctaaag agcttgaggc tttggcgtgt gcggcatcgg
     7021 cttggacgta aaagccggat gcgccttccg ccagtgcggc ggccgggagt gcgagggcaa
     7081 tcagtgcggc aagtgctttt ttcatatttt ggttccttta tggtcaggga gaaaaattgt
     7141 taataatccg ttaaagaatc ctgccgtatt atactgaaat tttctttttg catcgtaata
     7201 ttttcaatgc ctcaagatac gcggcggcat ccggctgctt tgccgacggc aaagccgtta
     7261 acccgcgcgt tgcccttaaa cggcggcatc acgcggcgga tgggtgaaac ttgcaaacgg
     7321 tttggaaaaa accgcaaagg cggggcggcg gcatttgtca gattgttgca ggcgcaggcg
     7381 tacggttttt tgtgtgcggc gttaccttag gcgtcggaca tttccggcgg cggctgtgcc
     7441 gtctgaaacg cccggcgggg gatgcggctg cgtttcccat cgataggcat atttgccggc
     7501 cgcgttcggg gcgggtttta cccgggcggc cgccgatttg tttgcgctta taaaaaacac
     7561 tgcaacaaat cgtttaaaaa caagcgtcct ttttcggtcg ggcggaatac ggtcgggtcg
     7621 gtttccagca ggcctttttg ccttgccgtt tcgatttgca ccatgatttt ggcggcgggt
     7681 acgcccgtgc gctcctgcaa catcgcggcg ggtacgccgt cggtcaggcg cagggcgttc
     7741 atcatgaatt cgaacggcaa atcttcggcg gcgacggttt tgcgttcgac ggcttcgccc
     7801 ggctgacttt gcattaaggc gaggtagtcg ttggggtggc ggcggcggac ggtgcgctcg
     7861 atgcggtcgg gataggagat tttgccgtgc gcgcccgcgc ctatgcccaa ataatcgccg
     7921 aactgccagt agttcaaatt gtggcggcac tgcatggtgg gtttcgcaaa agccgatgtt
     7981 tcgtagtgga taagacccgc gccttccagt gtgccgtgta ccgcgtcttc gatgtcgagc
     8041 gccgcttcgt cttgcggcaa acctttcggc ggcgtatgac cgaacggcgt gttcggctcc
     8101 atcgtcagat gatatgcgct gatgtgggtt gcgtctgtgg cgatggcggt ttgcacgtcg
     8161 tccaatgccg tctgaacggt ttggttcggc agggagtaca tcaagtcgat attgacttta
     8221 tcaaataatt ttaaggcggt atcgatagcg gttaaggctt ccttgccgtt gtggacgcgc
     8281 cccagccttg agagcatatc gtcgttgaag ctttgtacac caatagaaag ccgcgtgatg
     8341 cctgcgtctt taaatccttg aaacttctcg atttcaactg tacccggatt ggcttccaac
     8401 gtaatttccg cttcgggctg caagcgcaac agcgaacgca cgccgcttaa caaacggtca
     8461 atcgattccg cctgaaacag gctgggcgta ccgccgccga aaaatatcgt ttccaccggc
     8521 ctgccccaaa tattgggcaa ttcaagctgc aagtcggtca gcagcgcgtc gatataggcg
     8581 gcttcgggca atccgttttt caggctgtgg gaattgaagt cgcaatacgg gcattttttg
     8641 atgcaccacg ggatgtggat atagagcgac aggggcggca gggcggtgag tcgggtgcgg
     8701 tttggaaagg aaatggtgtg catggtgcgg ttcggaaaag tggacgatgc cgtctgaagg
     8761 cggttcggac ggcatgggtt cagcagagca gggtaagcag ttcggcttcg ctgaggacgg
     8821 aaacgcccaa ggcattggct ttttccagct tgctgcccgc ggcttctccg gcgacgacgt
     8881 agtcggtttt tttggacacg ctgccggaca ctttgccgcc tgcggcttcg attagggctt
     8941 gggcttggtc gcgtttgaag gtgggcaggg tgccggttaa cacaaaggtt ttgcctgcca
     9001 cggctttatt gatgccgtct gaaccttgcg ccgcctcgtc ttcagacggc atttgcgcga
     9061 agaaggtttt taggttttcg agcagggcgg tgttttgcgc tttgctgcgc cacgcctgcc
     9121 agtcggcagg gagggctttg tcgttttgca gtccttctat actttgtccg gcgagttccc
     9181 ataaggcttg ggctttgttt tcgctgattt tgaaaccggg caggcgggta atccagcgtt
     9241 gcggcccggc atattgtgcg gcaggcaggc tgacggcttg ggcttgcggg gcaacgcctg
     9301 cggcgagcag ctcgtctatc atcgcctgct gttcggcttg ggcgaagaag tgggcaatgg
     9361 aacgcgccac taccgtgccg atgtcgggca ggcaggcgag gacgggttcg ggggcgcggc
     9421 ggacgcgttc caatgtgccg aatgcctgcg ccagcgtttt ggcggtgcgt tcgccgacgt
     9481 ggcggatgcc gagcgcgaac aggaagcggg caagttcggg cgttttgccg gattctatgc
     9541 ctgcgaggat gttttgcgcc cacttggtcg gttgtttttt gccgttttgg gtattgtatt
     9601 tagacaggtc gcctgaaacc gtttctgcgt cgccgttttc gttttcagat gaccctttat
     9661 ccgccgtttc cttcattttt tgcagggtcg gaatatcgat gcggtagagg tcggcgaaat
     9721 ggcggacgag gtcttgggcg accagctgtt cgatttgttt ttcacccaag ccgtcgatgt
     9781 ccatcgcttt gcgcgaggcg aagtggatta agccttgcgc gcgttgtgcc tgacaaagca
     9841 taccgccgct gcatcgggcg acggcttcgc cttcttcgcg ttcgatttcg ctgcggcaga
     9901 tggggcagcg ggccggcagg cggtagggct tatggagcgg aacggattcg gtttgcttgg
     9961 cggacggtgt ttcggcaaac aggtcgtcct gctgatgccc gatgccgtct gaaacggcaa
    10021 cggcggtttc ctgcatcggg cggcgttcga aaatcacgcg cacgacttcg ggaatcacgt
    10081 ctccggcgcg gcgcacgacg acggtatcgc cgacgcgcac gtctttgcgc gatacttcgt
    10141 cctgattgtg cagggtggcg ttggtaacgg ttacgccgcc gacgaatacg ggttgcaggc
    10201 gggcaaccgg cgttaccgcg cccgtcctgc cgatttgcac gtcaatcgct tcgacaatgg
    10261 tcagggcttc ttcggcaggg aatttgtggg caaccgccca acgcggcgcg cgggagatga
    10321 agccgagttc gcgctgttgc gccaagctgt tgactttgac caccatgccg tcgatttcgt
    10381 agggcagttc ggggcgtttt tgctgcatgt gttcgtaaaa cgccaatact tcgccgatat
    10441 ttttgaaaca gccgaaattg ccattgggca gactgaagcc gagttcttgg aaataggcga
    10501 gttcttggat gtgttcttca gcttcgaaac cgccttgctg acgggcaatc gaatagggga
    10561 aaaagtgcag tttgcgttgc gcggtgatgc gcgaatcgag ttggcgcagg ctgccggcgg
    10621 cggcgttgcg cggattggca aagggttttt gcccgttttc ggcttggcgt ttattgagcc
    10681 cggcaaagtc ggctttgagc atcagcactt cgccgcgtac ctcgatgagt tcgggcgtat
    10741 tttcaccgtg cagccgcagg gggatgttgg cgacggtttt gacgtttcgg gtaacgtctt
    10801 cgccggtcgt accgtcgccg cgtgtcgccg cctgcaccaa tacgccgtcg cggtagagca
    10861 ggctgatggc gaggccgtcg aatttgggtt cgataacgta ttcgggattg ccgccgtcca
    10921 agccgtcgcg cacgcgttgg tcgaaggcgt acatttcggc atggtcgaac acgccgtttt
    10981 catcttgcgg ggaaaaggcg ttggtcagcg acagcatcgg cacttcgtgg cgcacttcgg
    11041 caaagcctgc caaaggctcg ccgccgacgc gctgggtcgg gctgtcgggc agcttgagct
    11101 cgggatggtt tcgttccaac gcttcgagtt cgcggaacag gcggtcgtat tcggcatcgg
    11161 gtatgctggg cgcgtcgagg gtgtagtatt cgtaggcgta gcggttgagg aggtcggtga
    11221 gttcgtgtat gcgttgtgcg gtcgggttca tggattcgta caggttcaga cggcatggac
    11281 aaatgccgtc tgaacggtaa aacgtgttgg aaatggtcgg attttacctg aaaacaaaag
    11341 gcggatgcac cgttgccgat gtatccgcct ttgtgtttgc ggagttatga aaacaggcgc
    11401 agggcggttt tgccgcccgg ttcgataccg actttgagca tctcggactg acgcgccaat
    11461 acataagtgc gtacgtcttt gagccattgg gtcgaaactt cttccatttt gtcgttgacc
    11521 agattcaggt tcaactgacc ggacaggcgt accgccaaat ccataaacaa atcgtcgaag
    11581 gttttttcgc ctgccggaga gtgcgggatg tcgagcagca tactgaagcc tttgtaggac
    11641 tggttgtcca aaagggcatt ggtaaacggc tcgttgttga gcgagcagat ggagaacatg
    11701 gtcgagcccg acgtgtcggt atagtggaac gcgccgtcgt cttccaaaac gaaacccacg
    11761 cccgttacgg cggaacgcag ttctacgccg ctgatgctgg tcggcgaaac caaatggatg
    11821 gcgatggtct ggtcgacgcg cgcgcagaat gcgtccagtg cggaagccac ttcgataaag
    11881 gcggcaaggt cggtgtgcag cgtctgaccg cccatgcttt gtgcgaatgc gtccgcctgg
    11941 cggttgaatg cggagagttc ttcctgcgag gcaagtccgt tgcggctgac tgcctggata
    12001 cccacgataa atgcctgata gcggatgccc gggatgggtt cggcaatctg gaaatggtcg
    12061 tccatggtgc agccgacaat ctggtagcgg cagcggttgg aaaggcgcgg cagtgcgtgc
    12121 agttctttgg cttcggtcag cgcgatatag gagatgaagt cgaagcgcac gtcaaaccag
    12181 ggcagctcga ccttcgacag ctctttgagc gtaatcagcg gttttgccgg cgtttgcgga
    12241 acgggaacgg gttttgccgg cgcgtcggca ggtttcggtg cggaatgtcc ggtttggggt
    12301 tcggaaacgg tgtgggcgga gttgccgata atgccgattt cttccaaggc ggtttcgatt
    12361 tcggttttga acggggaggc ttttgcctgt ttctgcttgg cgatgtagac ggcatcctgt
    12421 tcttgcaggt tgcgcatggc ggagtcttgg ggtttggccg gttttttgac cgccggttgg
    12481 ggtttcggca tcatgactgg cccgccggac ggtttgccgt cgcggacatg gctggttttg
    12541 ctgttgagca gggcatcttt gtcggagtgt ccgaactggt cgcgcacttt tttgcggtat
    12601 tggttttcct gatacatatt gtaggcgaca acggcgagga cggcggcgag gaacagtacg
    12661 atgtaaatca tggcaatcac tttaaatttc gggatgcagg atgcgcaaag tgcgggtact
    12721 gcggttaaat cgggcttgcg tttccggcag tctgacggaa cggccggtta taacgtttga
    12781 attataacga aaattgcagg gtctgacagc agtgtgtcga aataagcgga aattttccga
    12841 aatgccgtct gaaatctgtg gttttcagac ggcatttctg tccacgggaa accctttttc
    12901 ccgtatccgc cgccagtcga aaaaatggcc ggggtcggtt ttgcggccgg gtgcgatgtc
    12961 ttggtgtccc gttaccgccg tgatggggta gcggcggcag agggctgcca acagtgtttc
    13021 gagcgaacgg tattgcgctt cggcaaacgg ttcgaaatcg cagccttcca gttcgatgcc
    13081 gatggaaaac gcgttgcact tctcccgccc ccgaaacgag gatgcgccgg cgtggtacgc
    13141 catgtcgccg catgatacga actgcaccgt ttcgccgtcg cgtgcaatca ggaaatggct
    13201 ggacacgtgc aaagtgtgta tcaggctgaa gaacggatgt ccgtcggggt cgagccggtt
    13261 ggcaaacagc ttttccaccg catccgtgcc gtattcgaac ggcggcagcg aaatgttgtg
    13321 cagcacgatc agggaaaccg tttctcccgt ttccctcggg ctgaaattgg gcgacggggt
    13381 atggcgtatg ctttgaagcc agccgttttg ccagtgtgct ccggcgtgat tgtccatgat
    13441 gttcttcctg tccggcgggc aatttgggtt atactgtcgc ccgaatttta agacgtattc
    13501 cgaatgctgg gaatcctacc atgttgaaaa aaatgttgaa atggactgcc gtttttttga
    13561 ccgtatcggc agccgttttc gccgcgctgc tttttgtccc taaagacaac ggcagggcat
    13621 accgaatcaa gattgccaaa aatcagggta tttcgtcggt cggcaggaaa cttgccgaag
    13681 accgcatcgt gttcagcagg catgttttga cggcggcggc ctacgttttg ggtgtgcaca
    13741 acaggctgca tacggggacg tacagattgc cttcggaagt gtctgcttgg gatatcttgc
    13801 agaaaatgcg cggcggcagg ccggattccg ttaccgtgca gattatcgaa ggttcgcgtt
    13861 tttcgcatat gaggaaagtc atcgacgcaa cgcccgacat cggacacgac accaaaggct
    13921 ggagcaatga aaaactgatg gcggaagttg cgcccgatgc cttcagcggc aatcctgaag
    13981 ggcagttttt tcccgacagc tacgaaatcg atgcgggcgg cagcgatttg cagatttacc
    14041 aaaccgccta caaggcgatg caacgccgcc tgaacgaggc atgggcaggc aggcaggacg
    14101 ggctgcctta taaaaaccct tatgaaatgc tgattatggc gagcctgatc gaaaaggaaa
    14161 cggggcatga ggccgaccgc gaccatgtcg cttccgtctt cgtcaaccgc ctgaaaatcg
    14221 gtatgcgcct gcaaaccgac ccgtccgtga tttacggcat gggtgcggca tacaagggca
    14281 aaatccgtaa agccgacctg cgccgcgaca cgccgtacaa cacctatacg cgcggcggcc
    14341 tgccgccaac cccgattgcg ctgcccggca aggcggcact cgatgccgcc gcccacccgt
    14401 ccggcgaaaa atacctgtat ttcgtgtcca aaatggacgg cacgggcttg agccagttca
    14461 gccatgattt gaccgaacac aacgccgccg tccgcaaata tattttgaaa aaataaacca
    14521 tgccgtctga aaagtttgtg ttttcagacg gcataccctt accggaactg caagcatgaa
    14581 accgcaattc atcactttgg acggcataga cggtgccgga aaatccacca accttgccgt
    14641 catcaaggca tggtttgaac ggagggggct gcccgtgctg ttcacgcgcg agccgggcgg
    14701 aacgccggtc ggtgaggcct tgcgcgaaat cctgctcaac cctgaaacca aagccggttt
    14761 gcgtgcggaa acactgatga tgttcgccgc gcgtatgcag cacatcgagg aagtcatcct
    14821 gcccgcgctt tcagacggca tccatgtcgt gtccgaccgt tttaccgatg cgaccttcgc
    14881 ctatcagggc ggcgggcggg ggatgccgtc tgaagacatt gaaattttgg aacattgggt
    14941 gcagggcggt ttgcgccccg atttgaccct gctgttggat gtgccgctgg aagtatcgat
    15001 ggcgcgtatc ggacaggcgc gcgagaaaga ccggttcgag caggagcagg cggatttctt
    15061 tatgcgtgtg cgcggcgttt atctcgaccg agccgccgcc tgtcccgaac ggtacgccgt
    15121 tatcgacagt aaccgcagct tggatgaagt cagaaacagc atagaaaaag tgttggacgg
    15181 acatttcggc tgttgatgcg gcaaatattg aaacaagcgc atccgcccgc gccgaaaacc
    15241 aaacggcagt gccgcaggtg aaaatggcgg tatgcgccaa actttcggca tgatagaatt
    15301 acgctcggtt acaaggcagg atgcgtcggc aatattaacg aaccgcccgt aacatgatga
    15361 cccgaaagcg tttcggacag ttcgattcaa atctttttct cgcaacagga ttgacacatg
    15421 gaaaactcat tgaaagaagc cgccctcaag ttccacgaat tacccgttcc gggcaaaatt
    15481 tccgttaccc cgaccaaatc tctggcgacc gacaaagatt tggcgttggc gtactctccg
    15541 ggcgtagccg ccccctgtat ggaaatccat gccgatccgc aaaatgccta caaatacacc
    15601 ggcaaaggca acttggtcgc cgtcatttcc aacggtacgg ccgttttggg cttgggcgac
    15661 atcggcgcgc tggcgggcaa acccgtgatg gaaggcaaag gcgtattgtt caaaaaattc
    15721 gccggtgtgg acgtgttcga catcgaaatc gacgaaaaag acccgcaaaa actggtggac
    15781 atcatcgccg ctttggagcc gactttcggc ggcatcaacc tcgaagacat caaagcaccc
    15841 gagtgttttt acatcgaacg cgaattacgc aaacgctgca aaatccccgt atttcatgat
    15901 gaccagcacg gcacggccat cattaccgcc gccgccgtat tgaacgccct gcgttatacc
    15961 ggccgtaaaa tcgaagaagc gactttggtg tgctccggcg caggtgccgc cgcgattgcc
    16021 tgcctgaacc aactgctgga tttgggcttg aaacgtgaaa acgtaaccgt ttgcgactcc
    16081 aaaggcgtga tttaccaaac ccgcgaagac aaagaccgca tggacgaatc caaaaaattc
    16141 tacgccgttg aagacaacgg ccggcgcgta cttgccgatg cggttaaagg caaagacatc
    16201 ttcttgggcc tctccggcgc aaacctgctg acgcctgaaa tgttgaacac gatgaacgaa
    16261 aaacccatcg tgttcgccat ggccaacccg aatccggaaa tcctgccgcc gctggcgaaa
    16321 gaaacccgtc cggacgtggt tatcggtacc ggccgctccg acttcccgaa ccaagtgaac
    16381 aacgtattgt gcttcccgtt catcttccgg ggcgcgttgg atgtcggcgc aaccaccatc
    16441 aacgaagaaa tgaaacgcgc ctgcgtgtat gctttggcag atttggcgat ggaagaagta
    16501 accgaagaag tggttgccgc ttacggtaag aaattcgaat tcggcgcgga atacctgatt
    16561 cctactccgt tcgattcccg cctgctgcct cgcgtcgcta cggctgccgc caaagcagcg
    16621 atggaaagcg gcgtggcaac ccgtccgatt gcagatttgg aagcttacgc tgccaagttg
    16681 ggcgaatgga agctgtaagc cgtttgtggt taaaatgccg tctgaactgt tttcaggcgg
    16741 cattttgttg tcagattgat aaatgaaaga tactggaaaa tgaaagagat gaaacctgtc
    16801 cgttatcata ttggcgatat acccgaaact tcaaaacaaa ccgcccccgg tcatgacgac
    16861 agggcagtgg gtgttgacga tgattgtttt catgattcct ttggtcaata tcgttatgtt
    16921 ttttgtttgg gcgttcggca gaggcaaccc caaccgtgcc aatttctgta aagcactgtt
    16981 cttatttacc ttgttggttc gcttatcggt ttgattttca tattgcttat aggtgggtct
    17041 gcatcgggta cgcattatta atgtgccggc tgattctgct tcgaagattt gtatcgaata
    17101 tgccaaattt ttttaaattt cataccgtta tcgaacggca ttggcaaaaa ccttatccgg
    17161 ttttgtcttt tctgctcaag ccgctttccg ggctgtttgc caaaattgcg gcaaaatggc
    17221 gggcggattt tttatcggga aaacggcaaa gcgaaaagct gtccgtgcct gtggtcgtgg
    17281 tcggcaacat ccacgcgggc gggacgggca aaacgccgat tgccgccgcg ctggtgtcgg
    17341 gcttgcagga aaaaggtgtc aaggtcggca tcatcagccg aggctacggg cgcaagagca
    17401 aggcggttta tgtattgaat gccgcgagcc gtgcggaaga tgcgggcgac gagcctttgc
    17461 tgctgttccg taaaacaggt gcgccgacgg cagtgggcag cagccgtgtg gaggcaggca
    17521 gggcgttgct ggcagcgcat ccggagcttg aattgattgt ggcggacgac ggtttgcagc
    17581 attacgccct gcaacgcgat gtggaaatcg cggtgtttcc ggcggcggat acggggcgta
    17641 cggatttgga cttgctgccc aacggcaatt tgcgcgaacc tttgtcgaga ttggaaagcg
    17701 tggatgcggt ggtggtcggc ggcagggcgg cggatggttt tatgccgtct gaacatttgt
    17761 tcggcagccg tattgaggcg ggtgcggttt accgtttgaa ccgtccgtct gaaaaactgg
    17821 atatttcgac attgtcaggg aagcgcgtcg cagcggttgc cggtatcgcc aggccgcagc
    17881 gtttcttcga tactctgaca cacatgggca tccgccttga ccaaacggtt gctctgcccg
    17941 accatgccga tattttcaat cgggatttgc cgcctgccga tgtggtgctg gtaactgaaa
    18001 aagatgcggt caaattttca gacggcattt gcaccgataa tgtttgggtg ctgccggttt
    18061 gtgcgataat cgaacctgat ttggcggagt ttgtgctgga gcggttggag ggtgtaccga
    18121 aggccgtctg aaagcgcggt ttggacgaag tgataacgga ctggaataag aacgccctac
    18181 gccgtcattc ccgcgcaggc gggaatctaa gtctcgaatt ttcaggaatg cctaggagac
    18241 ttcagaaatc ccaaatctcc ggatttccac ttgggcagga atgagaaagc cggttgtatt
    18301 ttttatctgc attaatcatt cgttaaagga gtggatatga agctgaaaac cttgttattg
    18361 cccttcgccg cactggcatt gtgtgccaac gcatttgccg ccccgcccgg cgacgcgtcg
    18421 ttggcacgtt ggctggatac gcagaatttc gaccgggata tagaaaaaaa atatgattga
    18481 aggctttaat gccggattta aaccgtatgc ggacaaagcc cttgccgaaa tgccggaagc
    18541 gaaaaaagat caggcggcag aagcctttaa tcgttatcgt gagaatgttt tgaaagattt
    18601 gattacgccc gaagtgaaac aggctgtccg caatacctta ttgaagaatg cccgtgaaat
    18661 atacacgcaa gaagaaattg acggcatgat tgccttttac ggttcgcctg tcggtcagtc
    18721 cgtcgttgcc aaaaatccgc gcttaatcaa gaaatcgatg agtgaaatag cggtatcttg
    18781 gactgcattg tcagggaaaa tcgcgcgaca tcatctgccc gagtttacgg aagagttacg
    18841 gcgcatcatc tgcggc
//
LOCUS       DAXDZG010000042        18387 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXDZG010000042 DAXDZG010000000
VERSION     DAXDZG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.042"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            42..115
                     /locus_tag="ACFB78_001254"
     tRNA            42..115
                     /locus_tag="ACFB78_001254"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Cys,seq:gca)
     gene            216..305
                     /locus_tag="ACFB78_001255"
     tRNA            216..305
                     /locus_tag="ACFB78_001255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Leu,seq:taa)
     gene            409..1461
                     /gene="sohB"
                     /locus_tag="ACFB78_001256"
     CDS             409..1461
                     /gene="sohB"
                     /locus_tag="ACFB78_001256"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC1269241.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(1512..2366)
                     /locus_tag="ACFB78_001257"
     CDS             complement(1512..2366)
                     /locus_tag="ACFB78_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC1269242.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMG
                     ILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWS
                     ERNIKAGT"
     gene            complement(2377..2808)
                     /locus_tag="ACFB78_001258"
     CDS             complement(2377..2808)
                     /locus_tag="ACFB78_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC1269243.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(3043..3913)
                     /locus_tag="ACFB78_001259"
                     /pseudo
     CDS             complement(3043..3913)
                     /locus_tag="ACFB78_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(3989..4900)
                     /gene="hisB"
                     /locus_tag="ACFB78_001260"
     CDS             complement(3989..4900)
                     /gene="hisB"
                     /locus_tag="ACFB78_001260"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC1269244.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(4917..5996)
                     /gene="hisC"
                     /locus_tag="ACFB78_001261"
     CDS             complement(4917..5996)
                     /gene="hisC"
                     /locus_tag="ACFB78_001261"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC1269245.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(6042..7331)
                     /gene="hisD"
                     /locus_tag="ACFB78_001262"
     CDS             complement(6042..7331)
                     /gene="hisD"
                     /locus_tag="ACFB78_001262"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC1269246.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRS
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(7328..8215)
                     /locus_tag="ACFB78_001263"
     CDS             complement(7328..8215)
                     /locus_tag="ACFB78_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC1269247.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(8307..8972)
                     /gene="hisG"
                     /locus_tag="ACFB78_001264"
     CDS             complement(8307..8972)
                     /gene="hisG"
                     /locus_tag="ACFB78_001264"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC1269248.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(9051..9704)
                     /locus_tag="ACFB78_001265"
     CDS             complement(9051..9704)
                     /locus_tag="ACFB78_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC1269249.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            9980..10064
                     /locus_tag="ACFB78_001266"
     tRNA            9980..10064
                     /locus_tag="ACFB78_001266"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10014..10016,aa:Leu,seq:caa)
     gene            complement(10127..10234)
                     /locus_tag="ACFB78_001267"
                     /pseudo
     CDS             complement(10127..10234)
                     /locus_tag="ACFB78_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10738..12465
                     /gene="ilvB"
                     /locus_tag="ACFB78_001268"
     CDS             10738..12465
                     /gene="ilvB"
                     /locus_tag="ACFB78_001268"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC1269250.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            12476..12967
                     /gene="ilvN"
                     /locus_tag="ACFB78_001269"
     CDS             12476..12967
                     /gene="ilvN"
                     /locus_tag="ACFB78_001269"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC1269251.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            13034..13327
                     /locus_tag="ACFB78_001270"
     CDS             13034..13327
                     /locus_tag="ACFB78_001270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC1269252.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            13407..14420
                     /gene="ilvC"
                     /locus_tag="ACFB78_001271"
     CDS             13407..14420
                     /gene="ilvC"
                     /locus_tag="ACFB78_001271"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC1269253.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(14539..15534)
                     /locus_tag="ACFB78_001272"
     CDS             complement(14539..15534)
                     /locus_tag="ACFB78_001272"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC1269254.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(15676..18261)
                     /gene="acnB"
                     /locus_tag="ACFB78_001273"
     CDS             complement(15676..18261)
                     /gene="acnB"
                     /locus_tag="ACFB78_001273"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC1269255.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         4114 a         4475 c         5129 g         4669 t
ORIGIN      
        1 ctcgtttccc gctccaagtt ttgtagacaa gccagtttta gggcgagata gcaaagtggt
       61 tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc cgactctcgc ctccatatat
      121 cttacggcgg ggtggcagag tgtttatgct atgaggggtg caaccttcat ataggccggt
      181 taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa taggtagaca
      241 caacggactt aaaatccgtc gggactaaac atcccatgcc ggttcgattc cggctccggg
      301 caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg tcggatgggg
      361 atggacaata ttgatatgtt catgcctgaa caagaggaaa tccaatcaat gtggaaagaa
      421 attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg cgcaattgcg
      481 ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag tgtcgtactg
      541 acagattttt cggaaaatta taaaaaacag cggcaatcgt ttgaaacatt ctttttaagc
      601 gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa aggaaaaggc ggaagccaaa
      661 gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg ccgaaacgca aaaatcccgc
      721 ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc cttgcgtcat
      781 gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct cagattggaa
      841 agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag gcgtttgcgc
      901 gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg cggcaagcgg cggctatatg
      961 atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattatcgg ttcggtgggt
     1021 gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa aacatgatat tgatgtggat
     1081 gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa tacggaaaag
     1141 ggcaaacaga aattccggca ggaactggag gaaacgcatc agttgttcaa gcagtttgtc
     1201 agtgaaaacc gccccgggtt ggatattgaa aaaatagcga cgggcgagca ttggttcggc
     1261 cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga tttgttgttg
     1321 aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagcg aagcctgatc
     1381 cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt tgtttgccaa acttgtcaac
     1441 cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga agtgtgttca
     1501 gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat ccgttgtccg
     1561 atgtctttca aattggaata ttgttcgacc aatcgcggca catcgtccaa actcatggca
     1621 aacatccaca gataagtgcc gaccgaataa atatgccccg cgctgccgta gcctttgagc
     1681 gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac gcagagatag
     1741 ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg aaaccagtcc gtaatggcgg
     1801 tacagctgcc gctcgtcgaa gtggttgtcg cgttccaagc tgttgttcag gcggaaatac
     1861 aggttttcgc tgatggcggc aaaacgtggc aaaagccata aaaacaacgc aagtatgccc
     1921 accgccgaca cgccgaccca aaattccagc accagcagca tgatgcacgc gccgaatatg
     1981 gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa aaaagctgac aaattcacgc
     2041 gacagggcaa cccgtgcagt taccgctgaa tgcgggactt gccgctgccg ttgttccaac
     2101 acaaccggca cggcgatttc ggtataaatc cgcgtaaacg tgcgcgtatc ggcaatccgc
     2161 cgtgccgcac cgaccagcca catcaaaaat acaaccaaag cgtacagcaa cgcctgccac
     2221 accctccccg caatcacggc attaatcgcc cagccgccaa acaccggata ccccagcatc
     2281 aaaaggtttt ccagtccgac cggggaaaat gtgccaatca atcgcttgcg gtgggttttg
     2341 gctatgtgtt tcaacatttt ccacatattt gtccctctat tttttatttt ccgagattgt
     2401 tttttccatc acttccgcca gtgcatccaa atcggcaaac agccgggttg tgcgcttgtc
     2461 gccgaatgtg gaaaatactt tgtcgctgaa ttcctgcgcg ttttctgtta aaggtgcggc
     2521 atggactttg cctttttcgg tcagcgacag caaccgttcg cgcctgtcct gttcgccttc
     2581 ctgccattca atcaaccctt gtccggcaag ggttttgcat acgcctgaaa cggtctgttt
     2641 gggcagactc cactcttctc cgatgtgctt ttgcgtgcgg ctgccttcgg ttgccagggt
     2701 ataaagtacg gtaaagaggt tgtaattcaa atcctgctgc ccgatccatt tgtcaaagac
     2761 attgcaaatc aggttgatac ggattccaag ttggtcgagt cggttcataa ttggtcttga
     2821 tattgactaa atttgtgcgg attatagtgg attaacaaaa accggtacgg cgttgcctcg
     2881 ccttgtcgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     2941 cactatatag tcataatcgg gactgatttc aagcgtgggg cgataaaaat gccgtctgaa
     3001 acgctttctg gcttcagacg gcatattgtt tggaaaggga agtcagtgtt ctgccaattt
     3061 caggtaaacg ccggaaacgt cctgttcgcc gtagccggct tcaactgctt tgcggtagct
     3121 ggcagcaacg gtttcgacgg cgggcagggt gttgcctgcc tgttcaagtt ctttgacggc
     3181 gaggttaagg tctttggaag cgtgtttgag tgcaaaggca gggggggaac tcacggtttg
     3241 cccatagtga tttttttgtt tgaaacatag gcgagtccat tgccgagccg ccgatggctt
     3301 cgacgatggt gtcggtatcg atgccgaact gccgcgccat cagcatcgct tcgctgtacg
     3361 cttcgccgaa aatgcctaag agcgagttca agacgagttt cgcgcccgag cctttgccga
     3421 catcgccgaa atggaaggtt tttttgccga caagggaaaa tattttttgc agcgggttta
     3481 aaacggcttc gctgccgccg aacagaatca gcagtgtgcc gttggtggcg ggtccgaccg
     3541 atccggaaac gggtgcttcg gcaaactgtc cgcctgcggc ttcgacaagt gctttgacgg
     3601 cgaggttttc ggtcggggag atggtgctca tgttgacgat gattttgccg gccaatccgt
     3661 cgcggactcc gttcaggatg tcgcacacgg cggcatagtc ggaaaccatc aggaaaatga
     3721 cggggcaggc gcggacgagt tcggcggtgc tgccgtaaac ttttgctcct ttggcggaga
     3781 tgggggcagt tttgtcgggc gagcggttgt atacgccgac ttcgatgccg ccgtccaaga
     3841 gccgcgttac cataggcaga cccatttgcc ctaagcctac ccagccgatt tgtgtgtatg
     3901 tttctgcgga catagtgttt ctcctttgtt ggggcgctgt gatgccgtct gaaggcttcc
     3961 tgcgcttcag acggtctgtt tggggatgtt atgcggtcag cgtgcctttg gtcgaggggg
     4021 tttgcccagc cattctcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     4081 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     4141 tcatcatgct gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     4201 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     4261 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     4321 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     4381 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     4441 tgccgtggcg ggcgatttgg tcgatcatgt gttcgaggaa gggaacgccg gtatccagcc
     4501 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     4561 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt cgggtttcgg
     4621 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     4681 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     4741 cgcgcgcatc ggggtcttct gcctgctttt caaggcgttg tttgagtttg taaagtgcga
     4801 cgggggtacg gtagccgcag agtttggcaa gcttgggcag cgaacctgct tcttgggcga
     4861 gagtcaggaa gttgttcagg tggagttgtg tcttggtcat agggattccg tttggtttat
     4921 cggtagagcc ggcggataac gtcgaggacg gcatcgtttt gtgcggagct gcctatggta
     4981 atgcgcaggc agtgttccaa aagcggatgt gcgccgtgca gttttttgac gaggatgcgg
     5041 ttttgtttga gtgtgtcaaa caacaaatcg gcatcgggta cgcgtatggt aatgaagttt
     5101 gcctgacttg gaaaggcgtt cagacggcat attttgccca attcggcgaa catccgttcg
     5161 cgttcgtttt tcaggctgtc gatgttggca gaaatgatgc cgtagtgttg cagggcgagt
     5221 ttggcggtgg tcaggctcag ttggttcata ttgtagggcg gcaggatttt ttgcagttcg
     5281 ccgatgactt cgggacagcc tgtcgcataa ccgatacgca gtccggcaaa accgattttg
     5341 ctgagggtgc gtaagactat caggttggga atcctgcccg cctgcggcag gaagctgtcg
     5401 ccgttgaatg cgccgtaggc ttcgtcgacg acgacgatgc cgtctgaagc ttcgatggcg
     5461 gcttcgattt cggcacgcgt gaaacatacg ccggtcgggt tgttcgggta ggcgataaag
     5521 gtcagggcag ggcggtgctt cctgacggct tcgaggacgg cgggcaggtt gagggtgaaa
     5581 tctccgttca gtggaacgcc gacataatcc atgccgtaca gcgcggcgtt gtggcggtac
     5641 atgatgaaac cgggttcggc tgccaacatt gccgcgccgg gtttggcggt cagcatggtg
     5701 atgaactgta tcagttcgtc cgaaccgttg cctaaggcga cggcggcgca gtcagggatg
     5761 tcgaacgccg aacgcaatgc ttcctgcaaa ccgcagccgg aggggttggg atagaggtgg
     5821 atgggggcgg atgccagttg cgcccgccat tcccgcatca gggcttcgtg cccctcaaaa
     5881 ggatgggcgg ggctttccat cgcatcgagt ttggcaaaac cgggcggaac atcggtgatt
     5941 ttgtatgcgg acatggcgag gatgtcgttg cggatgaagg agcggacgga tttcatcgtg
     6001 tttccttaat ggttggaata tgcctgtacg ccgtttcggc attatttcat acggaactct
     6061 gccgcgcgtg cgtgggcggt caggctttcg ccgtgtgcca gcacgctggc ggtttcgcct
     6121 aatttttgcg cgccctgttc cgaaacctga atcaggctgg agcgtttttg gaaatcatat
     6181 gtccccaaag gcgaggaaaa gcgggcggtt cggctggtgg gcaacacatg gttcgggccg
     6241 gcgcagtagt cgccgaggct ttcgccggtg tagcgtccca tgaaaatcgc gccggcatgg
     6301 cggatttttt tcgcccattc ctgcgggttt tcgactgaca gttccaagtg ttcgggggaa
     6361 atgtagttgg agatttcgca ggcttcgttc aagtctttaa cgagtatcat cgcgcccctg
     6421 ttgccgagcg aggcctcgat gatgtcgcgg cgcggcatgg tttcgatgag cctgtccata
     6481 gcggcttcta cttcgtcgag atacgcttgc gacgtgccga tgaggatggc ttgggcaatt
     6541 tcgtcgtgtt cggcctggct gaacaaatcc atcgccaccc aatcggcggg tgtcgtgccg
     6601 tcggcgatga ccagtatttc agacggcccc gccaccatgt cgatgccgac cacgccgaac
     6661 acgcggcgtt tggcggcggc gacgaaggcg ttgcccggac cggtgatttt atcgacttgg
     6721 gggatggttt ccgtgccgta ggcgagggcg gcgatggctt gcgcgccgcc gacggtgaag
     6781 actttggtta cgccggcgac gtatgcggcg gcaagcacga tgtcgttgcg ttcgcctttc
     6841 ggtgtcggta cgaccataat gatttctttc acgcctgcga cgtgggcggg catcgcgttc
     6901 atgatgacgg aactcggata tgccgccttg ccgcctggga cgtaaatgcc gacgcggtca
     6961 agcggggtaa tctgttgccc cagcagcgtg ccgtcttcat cggtgtagct ccacgattcc
     7021 attttttggc gttggtggta gctttcgaca cggcgggcgg cggtctgcaa tgctgtctga
     7081 acgtcgttcg gaatgcgctc gaacgccgcg ttcaaatcgg cttgcgtgag tattaaatca
     7141 tcgatgcttt tagcgtttgt ctgatcgaat ttgttggtgt attcaatcaa agccgcatcg
     7201 ctgcgctttt gcacgtcggc gcaaatgtcg gcgacgatgc gttcggtttc ggggttttgc
     7261 gcggtttcaa aagccagcag ggctttgagt ccggcttgga aatcgggcga ttgggtgttg
     7321 agttttttca tgatttggga ttctcctttg ttattttggg acttgggatc gtctgaagcg
     7381 tctcttgctc ggttttgggt ctgatatacg cttttcctcc cccgctacca aacttcaatt
     7441 cgggcaggca tttttgttgc agaccgagtt tggctaatac ctcttcgcta cgagaggtag
     7501 tacggaaacg gtaacgggtt tgttttctgc cgtttttggt ttcctcagtc attgtttcaa
     7561 aaccgtccac ttcgtacgca ctgacaacag cgttttgcag accggtgtga acgccgataa
     7621 cgtatttcac ttttgatgca acatctttac cgataaccca gttgcccaac gtacgcgatt
     7681 tgaggttggc atcgtcttgg ttgtcgaaaa ggtagtctaa ttcttcgtca gtatctaaat
     7741 cgaaagcatt gtggatcttg atggcgagaa tcagcccgtc ggggttaatc tcgctaagtg
     7801 ggagagaaga gaatccaaag cggcgatcta gttcttctac gctaatacca cccggaccat
     7861 gcccggcaat tttatttttg agttttttac ccaagacaga tttaacaaaa tgaattaagg
     7921 cagattcggc ggcgagcgct tcgacttcag tcagatgata gctgatgata tagcgaccga
     7981 gtttcttgca tttggagatg gctttgagtt tccgatcgat aatctcgccg gagactgggt
     8041 cttgtgaacg actagcaacc cattcatgct cgaagatacg attaccgcaa ccttttccga
     8101 tgtagaggat tttgtctttt ttcaggtcgg tcagacaata gacataaaac cgacgctcgc
     8161 cgttattcaa aacggaaagg gtcgaatctg agaatttttt tatttttgca accattttaa
     8221 cttctcctat acagtttact gccggtgata ttttcagacg gcctgtcggc gggaacggat
     8281 agggtcgtct gaaaacgcat ctttattcaa atgaatgccc acttcgcctt cactgcgccg
     8341 ccgaacgact gaataatcgg ctccagcagc gcgtatttcg ttttcaaagc agccttgttg
     8401 accaccaggt agctggaaat gtcgacgatg tgttcgactg cctccaagcc gtttgccttc
     8461 aaggtgtttc ccgtcgaaac caagtccaca atcgcatcgc tcaagccgac cagcggcgca
     8521 agttccatcg agccgtacag tttgataatg tccacatgga cacccttgcc ggcaaaatga
     8581 gatgccgcga tttcaggata cttcgtggca atcttcagac ggcatccggg ttgcgaagcg
     8641 gcttcgtaat caaacccttt acgcacagca accatcatgc ggcacttggc aatctccaaa
     8701 tccaaaggtc ggtaaagccc cgtgccgccg tgttcgatca gcacgtcttt gcccgcaatg
     8761 ccgaagtccg ccgcgccgta gcggacataa gtcggcacat cggttgcgcg gacaatgaca
     8821 aggcggatgt tttcatggtt cgtcccgata atcagcttgc gcgatttttc gggctcttca
     8881 gtcggagcaa tgccggcagc ggcaagcagc ggcagcgtct cctcaaaaat gcgccctttg
     8941 gataaggcga tggtcaaagc attatcctgc atggtgtgga tgtcctttgt cggttgaaac
     9001 aataatgccg tctgaacagg caccggcggt gcagacggca tacggtttta tcagagcagg
     9061 gtccttacat cggcagcaat cgtttccggc tcgcttccgt aaggcgagaa aatggcaacc
     9121 tcaccgtttt tatcgataag atacgcaccg gaagagtggt cgaccaaata gttttcgctg
     9181 tcgtcttttt gattgatttt ggcagaaacc acgcggtatt gctgcttgat gaccggcagg
     9241 ttttgaccgc ccgttgccgt cagaccgata aagtccggat tgaactgttt ggcatacttg
     9301 ccgatgattt caggcgtgtc gcgttccgga tcgatgctga cgaacaccac tttcacgtcc
     9361 ttagcctgcc cgcccaactg cttcaaagtg tcgctgtacg tcaaaagctc tgtcgggcag
     9421 acatcgggac agtgcgtaaa gccgaaagac agaatcacga ccttgccttt caaatcgctc
     9481 aggctgaaag gcttgccttc gccgtcggtc agcgtgaaat cgccgccgat gtcttcctta
     9541 cgcatatccg taccgcgcgt ttgcggcttt gccgcatttt ccgcagccgg cgcggatgca
     9601 cttgaagaag cggcttgcgc cgcactgttg tcttgaggtt tgcaggcggc aagcgcggca
     9661 agtacgaaaa cgcccggcaa aaaggaacgc ggtacggaaa acatgacaat ctcccaagaa
     9721 taaatagaaa aacctgattc gtacggaatt tgaatatttc catcttaacg cgtttggcag
     9781 atttaatgta gtattttttt acgttgtccg tacggcagga attgccgaat catacagaat
     9841 ctttggagcg gaaagaacag taagattaaa ataataaaaa ttctatttaa aatcaaaaac
     9901 aaaataaaaa cagataaaat aatttcaaaa aaaggtttac agaatctcaa aatccattat
     9961 aatggcaatt tcttcaccag cccaggtggc gaaattggta gacgcagggg actcaaaatc
    10021 ccccgccgca aggtgtgtcg gttcgagtcc gaccctgggc accacaaccg cttttggagc
    10081 ggttattttt ttgtctattg ctgtttaatg aaatagataa gactaaatat agtggattaa
    10141 atttaaaccg gtacggcgtt gcctcgcctt gccgtaccat ctgtactgtc tgcggcttcg
    10201 tcgttttgtc ctgatttaaa tttaatccac tatacaatca atatatttaa gtcgttgtta
    10261 gccctcttgg ttctgtcaat gccgtccggt gcaatgagcc attttcagga agtatggttg
    10321 ggaatatttt tgatatatca aaaataaaat caagttcaat caatatcccg agtgaaaccc
    10381 tgtaaatttt aaaattcttg atactttcac atctgccccc atgcaaactg tcaacaattt
    10441 ccatctttca aaaaaggtac tcctgatttt attcttttaa aatcaatcaa taatttctcc
    10501 gctttcaaaa aatattaaaa gatgaaagat ttttcttgat tgaactgtcg gtttgcgtta
    10561 tcgtttttac tctttttcac aaactctgtg ttcctttcca attgattgga taatctgtct
    10621 gttgccgtgt tcccccaatg cgcgtaccct aaatcgctgc ttggtggaat tgcattacag
    10681 gtgctgtggc aaagcggttt gtccctattg gtttgaaacc gtataaaaga ggtcgttatg
    10741 cagttatcag gcgcgcaaat catagtgcag agtctcaaag ccgaaggcgt cgagtacgtt
    10801 ttcggttatc ccggcggtgc ggttatcgaa atctacgatg ccctgttcca actcaataaa
    10861 ttcaaacaca ttcttacccg ccatgagcag gcggcagtac acgcggcaga tgcgtatgcg
    10921 cgcgtcagcg gtaaggtggg cgtggcattg gttacatccg gcccgggcgt taccaatgcg
    10981 ctgaccggta ttgctactgc ctatacggat tcgattccga tggtggtcat cagcggacag
    11041 gtaggcaatt ccctgattgg tacggatgcg ttccaagaag ttgatacggt gggtattacc
    11101 cgtccgtgcg tcaaacacaa tttcttggtt acggacatca atgagttggt ggaaaccatt
    11161 aaaaaggcgt tccaaattgc cgcaagcggc cgaccggggc ccgtggtgct tgatgtcccg
    11221 aaagatgtta cgcaggcgat ggcgaaattc agctatcctc aggaagacat ttttatccgt
    11281 tcgtatcaac cggttgttca agggcatatc ggtcagatta aaaaggccgt gcagatgttg
    11341 gcatctgcca aacgcccggt cgtctatttt ggcggcggcg tggtgttggg taatgcttct
    11401 gaagagctga cccgatttgt ccgaatgacg ggtgctccgt gcacgggtac gctgatgggc
    11461 ttgggcgctt atccttccgg cgaccgccaa ttcctcggta tgctcggtat gcacggtact
    11521 tacgaggcaa accttgccat gcagaatgcg gatgtcgttc ttgccgtagg tgcgcgtttt
    11581 gacgaccgtg tggtttccgt accgtccaaa tttttcgaga aggcgaagaa ggttatccat
    11641 atcgatgtcg atccttccag catcgccaaa cgcgtgaagg cggacattcc gattgtcggc
    11701 gacgtgaaaa acattttgtc cgagatggtt gcgctgtggc aaaaacaaga gtccgtgccg
    11761 tctgaagatg ctttgggcaa atggtggaaa accatagagg aatggcgttc ccgcgattgc
    11821 ttgtggtttg acaacggcag cgaaattatc aagccccaat atgtgattca gaagcttgcc
    11881 gagattaccg gcaattcggc aatcatcaca tcggatgtag ggcagcatca aatgtttgcg
    11941 gctcaatatt atcctttcga acgtccgcgc caatggctca actccggcgg tttgggtacg
    12001 atgggcgtgg gtctgcctta tgcgattggt gcaaaacttg ccgccccgga tcaagacgta
    12061 ttctgtatta ccggcgacgg ctcgatccag atgaacatcc aagagttgtc cacctgtttc
    12121 caatatcgga ttccggttaa cgtcattacg ctgaacaacg gttatctggg tatggtacgc
    12181 cagtggcagg aaatatatta cggcggtcga gagtcggaaa cctatttcga ttctttgccc
    12241 gatttcgtca aacttgccga ggcatacggc catatcggca tccgcgtgga caagaaatct
    12301 gatgtggaag gtgccttgtt ggaagcattg aaccaaaaag acaggctggt gtttatcgac
    12361 ttcctgaccg accagaaaca gaatgtgatg cctatggtcg gcaacggcaa aggtttggac
    12421 gaaatggtac ttccgccgca tatgcgtacg gacggaaagg cgtaaggaga ggcaaatgcg
    12481 acatatctta tctgttctga tcgaaaacga atcaggtgcg atgagccgcg tggtcggttt
    12541 gttctctgca cgcgattaca atatcgattc tttggcggtt gcgccgaccg aagacaaaac
    12601 cctgtcacgg atgaccatcg ttacccacgg cgacgagcag gttatcgaac aaatcaccaa
    12661 gcaactcaat aaattgattg aagtgattaa agtggtcgat ttgaacgaaa gccgttttgt
    12721 cgaacgcgaa ctgatgttgg taaaagtccg tgccgccggc aaagaccgcg acgaattttt
    12781 acgcttgacc gaaatctacc ggggcagcat catcgacgta accgaccgca gctatacgat
    12841 tgaaattacc ggctcgaccg acaagctgga ttcctttttg gaaacggtcg gacgcgccca
    12901 aattttggaa accgtacgta caggtgcggc cggcatcggt cgcggtgagc gtattttgaa
    12961 aatttaacgc cgtaaccttt cagacggcat ggtatttgaa tgccgtctga aaaacgaacg
    13021 gcaggagaga tttatgtcga acattaaaat tgtcgcactg gttaccgtca aaccggaata
    13081 cacggaaaca ctggcagcac agtttaaaga actggtcaaa gccagccgtg cggaagaggg
    13141 caacatcagc tacaatctcc atcaggaaat cggcaaaccg aaccgttttg ttttcgtgga
    13201 aaattggaaa tcccaagcag ctattgacga acataatgcc agcgcgcatt tccaagcctt
    13261 cgtccagtcc gtcgacggca aaaccgaagc gttggaaatc gtattgatga atgaagttgc
    13321 cgtttaagcg gcacactctg tttaacccgt ccgaagccgc tcaacatttt aaggcttcga
    13381 caaccattta ccttaaagga aatcaaatgc aagtctatta cgataaagat gccgatctgt
    13441 ccctgatcaa aggcaaaacc gttgccatca tcggttacgg ttcgcaaggt catgcccatg
    13501 ctgccaacct gaaagattcg ggtgtaaacg tggtgatcgg tctgcgccac ggctcttctt
    13561 ggaaaaaagc cgaagcagcc ggccatgttg ttaaaaccgt tgctgaagcg accaaagaag
    13621 ccgatgtcgt tatgctgctg ctgcctgacg aaaccatgcc tgccgtctat cacgccgaag
    13681 ttgcagccaa tttgaaagaa ggcgcgacgc tggcatttgc acacggcttc aacgtgcact
    13741 acaaccaaat cgttccgcgt gccgacttgg acgtgattat ggttgccccc aaaggtccgg
    13801 gtcataccgt acgcagtgaa tacaaacgcg gcggcggcgt gccttctctg attgccgttt
    13861 accaagacaa ttccggcaag gccaaagaca tcgccctgtc ttatgcggct gccaacggcg
    13921 gcaccaaagg cggtgtgatt gaaaccactt tccgcgaaga aaccgaaacc gatctgttcg
    13981 gcgaacaagc cgtattgtgc ggtggcgtgg ccgagttgat caaagcaggt tttgaaaccc
    14041 tgaccgaagc cggttacgcg cctgaaatgg cttacttcga atgcctgcac gaaatgaaac
    14101 tgatcgttga cctgattttc gaaggcggta ttgccaatat gaactactcc atttccaaca
    14161 atgcggagta cggcgaatac gttaccggcc ctgaagtggt caatgcttcc agcaaagaag
    14221 ccatgcgcaa tgccctgaaa cgcatccaaa ccggcgaata cgcaaaaatg tttatccaag
    14281 agggtaatgt caactacgcg tctatgactg cccgccgccg tttgaatgcc gaccaccaag
    14341 ttgaaaaagt cggcgcacga ctgcgtgcca tgatgccttg gattacggcc aacaaattgg
    14401 ttgaccaaga caaaaactga ttgttttcaa acgggactgc ctacacatcg tgtaggcagt
    14461 ttgttatatg gataccgtct gaacatcgtg ttcggacggt atctgtgttg cggatgaatt
    14521 taaacaggca cagttctgtc agtcgcccag agccgcgacc attaccgctt taatcgtgtg
    14581 catacggttt tccgcctgat cgaacacgat gccggccgga ctttcgaata cttcttctgt
    14641 aacttccaca ccgttcagcc cgaaggtttc gtaaatccat tcgccgactt tggtttcgcg
    14701 gttgtggaag gcgggcaggc agtgcatgaa tttgacttgc ggattgcccg atgccgccat
    14761 cagttcgggc gtaacgcggt aatctttcag caaatcgatg cgttcctgcc agacttcttt
    14821 cggctcgccc atgctgaccc atacgtcagt atgaatgaaa ccgacacctt tgacggcttc
    14881 atgcgcgttt tcggtcaggg taatttttgc accggtttct ttggcggcgg cgtgtgcggc
    14941 ggcgataatg ccttcagacg gccacaggct ttgaggtgcg ccgatacgca cgtccatccc
    15001 caattttgcc cctaaaatca gcagggaatt gcccatgttg taacgcgcgt cgccgacgta
    15061 ggcaaacgcg gtttggttca aaggtttgcc gctgtgttcg cgcatagtca gtgcgtcggc
    15121 aagcatttgt gtgggatgga actcgttggt cagcccgttg aacacgggta cgcccgcata
    15181 ttttgccaat tcttcgacag tttcctgagc gaagccgcga tattcgatgg catcgtacat
    15241 tctgcctaag acgcgcgccg tgtctttgat gctttctttg tgcccgattt ggctggcgga
    15301 cggttccaga taggttgcat ccgcgccttg gtcgcgtgcg gcgacttcaa acgcacagcg
    15361 tgtgcgcgtg gatgtttttt caaaaatcag ggcgatgttt ttccctttca tccgctgaat
    15421 ctcgcgccct gcctttttgg cgtctttcaa ctcggcggca aggtcgaggt aggtggtgat
    15481 ttcttccggc gtgaagtcca aaagtttcag aaaatggcgg tttttcaggt tcactgtcgt
    15541 ttcctttatg tgggggcgtg aacgccgtat ttgcagcgtt tgcggaaaga agggttagcg
    15601 gaatgttgca atgccgtctg aaggttgttc agacggcatt gcggtcaatc ccttactcca
    15661 tacggatgac ggggatcaaa cattcacggt ctcggctact tcgttgtagc tgtcgatttc
    15721 gttgaagttc atatagcggt agattttatc gccctgttcg ttgatgatgc cgatattggc
    15781 ttgatattct tcaacggtcg ggattttacc cagtttggag caaatcgccg ccaactctgc
    15841 cgagccgagg taaacgaagg tgtttttacc caagcggttc gggaagttgc gggtcgaagt
    15901 ggacatgaca gttgcacctt cgcgtacttg ggcttggtta cccatacaca acgaacagcc
    15961 cggcatttcc atacgcgcgc cggcgcggcc gagtacgccg tagtgacctt cgtcggacaa
    16021 ctctttcgcg tccattttgg tcggcggcgc tacccacagg cggacgggga tgtcgctctt
    16081 gccttccaaa agtttggagg cggcgcggaa gtggccgatg ttggtcatac aagaaccgat
    16141 gaacacttcg tcgattttgg tgccggagcg ttcggacata aagcatacgt cgtccgggtc
    16201 gttcgggcag gcgataatcg gctctttgat gtcgtccatg ttgatttcaa tcacggcggc
    16261 gtattcggca tctttatccg cttcgagcaa ctcgggattt gccagccatt tttccatagc
    16321 tttgatgcgg cgttccaaag cgcgcggatc ttgatagccg ttggcaatca tatttttcat
    16381 caacacgacg ttggatttca tgtactcgat aatcggctct ttattgagct tcacggtaca
    16441 gccggcggcg gagcgttcgg cggatgcgtc ggtcaattca aaggcttgtt ccactttcaa
    16501 atcgggcagg ccttcgattt cgaggatgcg gccggagaag atgtttttct taccggcttt
    16561 ggcaacggtc agcaaacctt gtttaatcgc gtaaagcggg atggcgttca ccaaatcgcg
    16621 cagggttacg cccggttgca gcttgccgct gaaacgtacc aatacggact cgggcatatc
    16681 gagcggtatt acgcccgttg cggcggcaaa agcgaccaag cctgaacctg cggggaagga
    16741 aataccgatg gggaaacggg tgtggctgtc gccgcctgta ccgactgtat caggcaacag
    16801 caggcggttg agccacgagt ggatgacgcc gtcgcccgga cgcagggaca cgccgccacg
    16861 ggtagaaata aaggcgggca gttctttatg ggtttttaca tcgacaggtt tcggataggc
    16921 agcggtgtgg cagaaagact gcatcaccat atcggcggag aagcccaaac aagccaagtc
    16981 tttcaactcg tcgcgggtca tcggcccggt cgtatcttgc gagccgaccg tcgtcatgcg
    17041 cggttcgcag taagtacccg gacgcacgcc ttgtccttcg ggcagaccgc aggcgcgacc
    17101 gaccattttt tgcgccaagg tgaaaccgac tttgctttcg gcaggcgctt gcggcaggcg
    17161 gaatgcagta gaggcaggca gtttcagggc ttcgcgcgct ttggcggtca gaccgcgacc
    17221 gataatcagg ttgatacggc cgccggcttg cacttcgtcc agcaatactt gtgatttcaa
    17281 ttcaaactcg gcaacagtct cgccgttttt cacgatttta ccttcataag gaaggatatt
    17341 gacgacatcg cccattttca gcgcggaaac atcgacttca atcggcagcg cgccggagtc
    17401 ttcttgagtg ttgaagaaaa tcggcgcgat tttaccgccc aagcacacgc cgccgaagcg
    17461 tttgttcggc acgaacggga tgtcttcgcc ggtatgccaa atgacggagt tggtcgcgga
    17521 tttgcgtgaa gaaccggtac cgaccacgtc accgacgtag gcaaccggat ggcctttggc
    17581 tttgagttct tccaacaatt taatcggacc gacttcgccc ggtttgtcgg gcgtgatgcc
    17641 gtcgcgcggg tttttcagca tggccagcgc gtgcagcgga atatcgggac gactccacgc
    17701 gtcgggcgcg ggagagaggt cgtctgtatt ggtttcgccg tcaactttga agacggtaac
    17761 ggtgattttt tcgggaactt tggcacggga ggcgaaccat tcggcatctg cccaagattg
    17821 caaaacttcc tgcgcgtatt tgttgccttt ttcggctttt tcctgaacgt cgtggaagga
    17881 atcgaacatc agaagcgtat gtttcaagcc tttggcggca atgggcgcga gtttgtcgtc
    17941 gtccaagagt tcgattaagg cgtggatatt gtaaccgccg agcatcgtac ctaagagttc
    18001 ggtcgcatat ttgggggaaa ccagcgggct ggatgcgctg ccttcggcaa cggcagccag
    18061 gaatgaggct ttgactttgg cggcatcgtc cacaccgggc ggaacacggt gggcaagcag
    18121 ctcgaccaag aactcgcctt cgcctgcggg cgggtttttc agcagctcaa ccaaatcggc
    18181 ggtttgctgc gcattcaaag ggagggcggg aatgccgagg gcggcgcgct cggcggcggc
    18241 tttacgatag gcttctaaca tctctttgtt cctttttccg tttttctttt gtcgggttgc
    18301 aaatgatctg cgttaaatat tgtaaacaac atttgtaccg ctatcataga ctagttttaa
    18361 caaaaatgga actgttatga caggtaa
//
LOCUS       DAXDZG010000043        18362 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXDZG010000043 DAXDZG010000000
VERSION     DAXDZG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18362
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.043"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(223..1440)
                     /locus_tag="ACFB78_001274"
     CDS             complement(223..1440)
                     /locus_tag="ACFB78_001274"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC1269256.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB78_001275"
     CDS             complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB78_001275"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC1269257.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2452..2916)
                     /locus_tag="ACFB78_001276"
     CDS             complement(2452..2916)
                     /locus_tag="ACFB78_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC1269258.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3134..3910
                     /locus_tag="ACFB78_001277"
     CDS             3134..3910
                     /locus_tag="ACFB78_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC1269259.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3914..4045
                     /locus_tag="ACFB78_001278"
     CDS             3914..4045
                     /locus_tag="ACFB78_001278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269260.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            4155..5009
                     /locus_tag="ACFB78_001279"
     CDS             4155..5009
                     /locus_tag="ACFB78_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC1269261.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            5050..6258
                     /gene="pncB"
                     /locus_tag="ACFB78_001280"
     CDS             5050..6258
                     /gene="pncB"
                     /locus_tag="ACFB78_001280"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC1269262.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIKRSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6350..8068
                     /gene="argS"
                     /locus_tag="ACFB78_001281"
     CDS             6350..8068
                     /gene="argS"
                     /locus_tag="ACFB78_001281"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC1269263.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            complement(8004..8389)
                     /locus_tag="ACFB78_001282"
                     /pseudo
     CDS             complement(8004..8389)
                     /locus_tag="ACFB78_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8588..10006
                     /locus_tag="ACFB78_001283"
     CDS             8588..10006
                     /locus_tag="ACFB78_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC1269264.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(10459..11205)
                     /locus_tag="ACFB78_001284"
     CDS             complement(10459..11205)
                     /locus_tag="ACFB78_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1269265.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11189..11368)
                     /locus_tag="ACFB78_001285"
     CDS             complement(11189..11368)
                     /locus_tag="ACFB78_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269266.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(11384..12055)
                     /locus_tag="ACFB78_001286"
     CDS             complement(11384..12055)
                     /locus_tag="ACFB78_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC1269267.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12124..12795)
                     /gene="rpiA"
                     /locus_tag="ACFB78_001287"
     CDS             complement(12124..12795)
                     /gene="rpiA"
                     /locus_tag="ACFB78_001287"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC1269268.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12872..13354)
                     /gene="ispF"
                     /locus_tag="ACFB78_001288"
     CDS             complement(12872..13354)
                     /gene="ispF"
                     /locus_tag="ACFB78_001288"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC1269269.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13386..14075)
                     /gene="ispD"
                     /locus_tag="ACFB78_001289"
     CDS             complement(13386..14075)
                     /gene="ispD"
                     /locus_tag="ACFB78_001289"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC1269270.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14072..14806)
                     /gene="dnaQ"
                     /locus_tag="ACFB78_001290"
     CDS             complement(14072..14806)
                     /gene="dnaQ"
                     /locus_tag="ACFB78_001290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC1269271.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14962..16272)
                     /locus_tag="ACFB78_001291"
     CDS             complement(14962..16272)
                     /locus_tag="ACFB78_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1269272.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16262..16447)
                     /gene="ccoS"
                     /locus_tag="ACFB78_001292"
     CDS             complement(16262..16447)
                     /gene="ccoS"
                     /locus_tag="ACFB78_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC1269273.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16447..16746)
                     /locus_tag="ACFB78_001293"
     CDS             complement(16447..16746)
                     /locus_tag="ACFB78_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269274.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17097..18293
                     /locus_tag="ACFB78_001294"
     CDS             17097..18293
                     /locus_tag="ACFB78_001294"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC1269275.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         4287 a         5410 c         4573 g         4092 t
ORIGIN      
        1 gaatccgttt tttgagtttc gcttaagttc aataaatcct gaaactttaa tctcgccgtg
       61 aaaatgaatt ttctatttca atttgtcata tatagttaat ttttttaggg tattttattt
      121 tttgttccaa agtcaaaata tgccgtccga acgttcgggc ggcagacaaa acggcactgc
      181 ccgataaagg cagtgccgtt gtccgtttta aaccgtgaaa catcagccca aatcaaaggc
      241 tttatgcaat accctggttg ccagttccat gtatttttcg tcaatcaata cggaaacttt
      301 gatttcggag gtggaaatca tttggatgtt gatgccctct tcggcgagcg tgcggaagat
      361 tttggcggct acgccgacgt gcgaacgcat acccaaaccg actgcggaga ctttgcacac
      421 ggtgtcgtcg ccgtcgatag aagctgcgcc gatactgtct ttacgttccg acaggatttc
      481 caaagtctgt ttgtaatcgc cgcgcggtac ggtgaaagaa aaatcggttg tgccttcgct
      541 gccgacattt tggataatca tatcgacttc gatgttggca tctgcgaccg cgcccaaaat
      601 ctgataggcg acgccgggct tatcgggcac accgcgcacg ttgatgcggg cttggttttt
      661 atcgaatgcg ataccggtta cggcagctct ttccatgttg tcgtcctctt caaaggtaat
      721 taaggtgccg ttgccgccgt cttgcaggct gctcagtacg cgcaggcgca ctttgtattt
      781 tccggcgaat tctactgaac ggatttgcaa aactttcgaa ccgaggctgg ccagttcgat
      841 catttcttca aatgtaaccg tatccatacg gcgcgcttcg ggtacgacgc gggggtcggt
      901 ggtgtaaacg ccgtctacgt cggtatagat ttggcattcg tccgctttga gggctgcggc
      961 aagcgcaacg gcggaagtgt cggaaccgcc gcgtcctagc gtggaaatat tgccttcact
     1021 gctgatgcct tggaagccgg caacgatgac gactttgccg gcggcgaggt cggcgcgcat
     1081 tttttcgtca tcaatgcttt ctatgcgggc tttggtgtgg gcggtatcgg ttttgaggga
     1141 gacctgccag cctgtgtagc ttttggcatc cacgccgata tccttcaatg ccattgccaa
     1201 aaggccgatg gttacttgtt cgccggtagc taagacgacg tccagctcgc gcggatcggg
     1261 atgctcctgc atttcgtgcg ccaacgcaac cagacggttg gtttcgccgc tcatggcgga
     1321 tacgacgacg acgatatcgt gtccttcggc gcgggcttta gcgacacgtt tggccacgtt
     1381 tttaatgcgc tcgggcgagc ctacggatgt gccgccgtat ttatgtacga ttaacgccat
     1441 gtttcatgct ttcttgtgtg ggattggtcg ggcagcatgg tttgctggaa aagcggctat
     1501 tattactatt ttttacatga agttcaagaa cggactgcgc tttcccgcct gccgtttgac
     1561 agcggtcagc gaaaaacctg ttctttcaga tttttgacaa aatgccgtct gaacggtttt
     1621 cagacggcat acggacagta attaagcaac ggacaacgca ttttgctgat aacgcagcag
     1681 ttcgcctatg cctttttgcg ccagtgcaac cagtttgccc aactcgtcca aactgaatgg
     1741 cgcgccttcc gccgttccct gtatttcgat gattttcccc gatgcggtca tgacgatgtt
     1801 cacatcactg tcgcagccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt
     1861 cactacgccc gctgatacgg cggcaacagc ttcgaggatg gggttttcac tcaaaatgcc
     1921 gtctgaaacc agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc
     1981 cgtacgcgtg ccgccgtccg cctgaatcac atcgcaatca atcaggattt gtcgttcgcc
     2041 gagtttttcc atatccacga ccgcgcgcag cgaacgcccg atcaagcgtt ggatttcttg
     2101 tgtgcgtccc gactgtttgc ccgccgaagc ttcgcggcgc atacgcaaag cggttgaggc
     2161 aggcagcata ccgtattccg ccgttaccca gccttggttt ttgccgtgca gaaacggtgg
     2221 ggcattttcg tccacggaag cggtacaaat cactttggta ttaccgcatt caataaggca
     2281 cgaaccgtcc gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc
     2341 ggcgcgtgag gtgcggatat aatcaggcat actgccctcc cgttaaaaac agataaatta
     2401 aaaagcctta aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc
     2461 ttcggctttg ctacggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc
     2521 gaaactgccc atcaaaaggt gcatcagccc ggtgcgcccg tgcgtgccca acaccagcag
     2581 gccggcaccg ttttcatcgg cataatcgac caaatcctgc gccatttcgc gcgcaccctt
     2641 attggcaacc agcaggtgtt tgacggtatt tcccacaccc agttcccggg cggtgcgctc
     2701 ggcggcatcc aaaacctcgt tgccttgcgc gacggcggcg gcctcgtaac tttcgtgttg
     2761 caaaaattcg ggggcgagcg ccatatattc ggcagggttg gcaacgtgta ccaaagtcag
     2821 acgcgcaccg ttgaccccgg caagctcggc ggcgtgtttc agggcattga tggacgtttc
     2881 actgccgtca acggcaacga ccagatgttt gtacatatcg tattctcctt ttgcaccgtc
     2941 tcacggtgcc gtctcttcag gtttgcgcaa aggcagtctg cgccgtttca ttatagaccc
     3001 gccgtcgggc tttatacaac agccgaacag cctgactgct ttccagtata atatgccgct
     3061 ttcgtgcagt caggcatttt tttgccggct ttcgttcact ttttgatttg acgcaatctt
     3121 gcaggattcg accatgtccg acaacgcttt gacctcttcg cgacgcttcg gcggcatcgc
     3181 cagactctac ggagactctg ccttgacgca cttttcacag gcacacgtct gcgtggtcgg
     3241 cgtgggcggg gtcggctcgt gggcggtcga ggctttggcg cgcacgggca tcgggcgttt
     3301 gactttgatt gatttggaca acgttgccga atcgaatgtc aaccgccagc tgcacgccct
     3361 gaccggcgac ttcggcaaag caaaagttac cgccttgcgc gaacgtatta cacaaatcaa
     3421 tccgcaatgc gaagtatttg aaattgaaga tttcgttacc gaagacaatt tgccggaata
     3481 cttcggaaaa ggttttgatt tcgtcatcga cgcgatcgac caagtgcgcg tcaaagcggc
     3541 aatggcggct tattttgtgg aacgcaaaca accgtttgtc ctcagcggcg gcgcgggcgg
     3601 acagaaaaat cccgccctga ttcaaaccgc cgatttaagc cgcgtaaccc acgacccgct
     3661 gcttgccaac ctgcgctaca ccttgcggaa acgctacggc ttcagccgcg atactaagga
     3721 aaaaatgcgc gtgccgtgcg tgtattcgac cgaaaatatc atgccgccgc aatcgggcgc
     3781 agcgtgttct gccgatgccg cgccgcaggg cttgtcatgc gccggctatg gcgcaagtat
     3841 gctcgttacc gcttcgttcg gactgtattg cgcacaggcg gcggtggaac acatcgcggg
     3901 caaaaaataa gcaatgccgt ctgaaacagg attcagacgg catttgaaca aacgcggcaa
     3961 tcaagctgtc ggttcagaaa aaccaacatc ggcacgccta aaaccgtatt cacaatctac
     4021 tccttttcaa aatatttgca tttaaaagcc gttataatgc cgtccgaaca tctgcccgac
     4081 cacattatgc gtgaatgccg gcagattgtt ttcttttgta aacttatatt aaaattcact
     4141 taccaattca cgccatgccg cccatccctg ccctatccgc accatccgag cacactgtcg
     4201 catgggtatt cggccaaccc gttaccgatt tgccgcagga tttgtttatc ccgcccgacg
     4261 cgctgaaagt cgtattgggc agttttcaag gccctttgga tctgctgctc taccttatcc
     4321 gcaagcagaa catcgatgtt ctcgatattc cgatggtgga aattaccggg cagtatctgc
     4381 actatattgc ccaaatggaa gcctatcagt ttgatttggc ggcggaatat cttttgatgg
     4441 cggcaatgct gattgaaatc aaatcgcgcc tgctgctgcc gcgtaccgaa gccgtcgaag
     4501 acgaagaggc cgacccgcgt gccgagttgg tgcgccgtct gcttgcctac gagcaaatga
     4561 aactggcggc gcagggtttg gacgcgctgc cgcgtgcggg acgggatttc gcgtgggctt
     4621 acctgccgct ggaaattgca gccgagacga agctgcccga ggtttacatc gccgatttga
     4681 tgcaggcatg gttgggcatt ctttctcggg caaaacatac gcgcagccac gaagtaatcc
     4741 aagaaaccct ttccgtgcgc gcgcaaatga cggcaatcct gcgccgtttg aacgaacacg
     4801 ggatatgcag gtttcacgac ctgttcaatc ccgaacaggg cgcggcttac gtgatcgtca
     4861 acttcatcgc cctgttggag cttgccaaag aaggattggt cggaatcgta caggaagacg
     4921 gtttcggaga aatccgaatc agcctcaatc atgagggggc gcattcagac ggcattttcg
     4981 gcacacgggg cgggcgcgat gtgttctaat acgccccaag ccgccaccag aaatcgggag
     5041 acacgccata tgaccggcat catacattcg ctgctcgaca ctgacctcta caaattcact
     5101 atgctgcaag tagtcctgca ccagtttccg cagacgcaca gcctttacga attccgctgc
     5161 cgcaacgtct cgaccgtcta tccgcttgcc gacatcaggg aggacttgga agccgaactc
     5221 gacgcgctct gccggctgcg cttcacccac gacgaactcg gctatctgcg ctccctgcgt
     5281 ttcatcaaaa gcgactttgt cgattatctc gaactcttcc agctccaacg ccgctttgtc
     5341 gaagtcggca cagacgataa aggccgtctg aacatccgta tcgaaggccc gatgatacag
     5401 gcgatgtttt ttgaaatctt catcctcgcc attgtcaacg aactttactt ccgccggctg
     5461 gaaacccctg ccgttatcga agaaggcgaa cgccggcttc aagccaaagc cgcgcgcctg
     5521 aaagaaatcg ccgccgcaca gaatccggac gaaccgccct tcctgatttc cgacttcggc
     5581 acgcgccgcc gctacaagct cgcgtggcag gaacacgtca tccgcaccct gctcgaagcc
     5641 gccccaagca tcgtgcgcgg cacaagcaac gtttacctcg ccaaaaaact cggcatcacc
     5701 cccatcggca ccatggcgca cgaattcctc caagccttcc aagccctcga cgtgcgcctg
     5761 cggaatttcc aaaaggccgc gctcgaaagc tgggtgcacg aataccgggg cgatttgggc
     5821 gttgccctga ccgacgtggt cggtatggat gccttcctgc gcgatttcga cctctatttc
     5881 gccaaactct tcgacgggct gcgccacgac agcggcgacc cttacgtttg gggcgacaaa
     5941 gcctacgccc actatcaaaa gctcaaaatc gacagccgca ccaaaatgct gaccttctcc
     6001 gacgggctgg acatcaaacg ctcttgggca ttgcaccaat atttcaaagg ccgcttcaaa
     6061 accggcttcg gcatcggcac caacctcacc aacgatatgg ggcatacgcc cttgaatatc
     6121 gtgttgaaac tggtcgaatg caacgggcag tccgtcgcca agctctccga ctcgccgggc
     6181 aaaaccatga ccaacaacag caccttcctc gcctacctgc gccaagtgtt cggcataccc
     6241 gaacctagaa cgccgtaaac cggcagaaaa agcgtacaat tcctgtttcc gccgcataaa
     6301 atcttttaaa ataccgcctg atttgaattt aaccgaaaga ccgaacttca tgaacctaca
     6361 tcaaaccgtc gaacacgaag ccgccgccgc ctttgccgcc gcaggcatcg ccggcagccc
     6421 cgttgtcttg cagccgacca aaaatgccga acacggcgat ttccaaatca acggcgtgat
     6481 gggtgcggcg aaaaaagcca aacaaaatcc gcgcgaactg gcgcaaaagg tcgccgacgc
     6541 attggcgggc aacgccgtca tcgaaagcgc ggaagtggcc ggacccggct ttatcaacct
     6601 gcgcctgcgc cacgaatttc tcgctcaaaa cattcatgcg gctttgaacg acgcgcgttt
     6661 cggcgtagcg aaaactgccc aaccgcaaac cgtcgttatc gactattctt cgcctaatct
     6721 ggcgaaggaa atgcacgtcg gccatctgcg ctccagcatc attggcgaca gcatttcacg
     6781 cgtgttggaa tttacgggca acaccgtcat ccgccaaaac catgtcggcg actggggtac
     6841 gcagttcggc atgttggttg cttatttggt cgagcagcag aaagacaatg ccgcgttcga
     6901 gctggcggat ttggagcagt tttaccgcgc cgccaaagtg cgctttgacg aagaccctgc
     6961 ctttgccgac accgcacgcg aatacgttgt gaagctgcaa ggcggcgatg aaaccgtgtt
     7021 ggcgttgtgg aaacagtttg tcgatatttc gctctcgcac gcccaagccg tttacgacac
     7081 gctgggcttg aagctgcgcc ccgaagatgt ggcgggcgaa tcgaaataca acgacgattt
     7141 gcagcccgta gccgatgatt tggttcaaaa aggcctggcg gttgaggacg acggcgcgaa
     7201 agtcgtgttc ttggacgagt tcaaaaacaa agagggcgaa cctgccgcat ttatcgtgca
     7261 aaaacaaggc ggcggcttcc tttatgcttc caccgatttg gcgtgcctgc gctaccgcat
     7321 aggccgtctg aaagccggcc gcctgctgta cgtcgtcgac caccgccaag ccctgcactt
     7381 cgagcaactc tttaccactt cccgcaaagc aggctatctg cctgaaaatg caaaagccga
     7441 gtttatcggc ttcggcacca tgatgggcaa agacggcaaa ccgttcaaaa cgcgcagcgg
     7501 cgacaccgta aaactggttg acctgctgac cgaagccgtc gagcgcgcca ccgctttggt
     7561 gaaagaaaaa aatcccgaat tgggcgcgga tgaagccgcc aaaatcggca agaccgtcgg
     7621 catcggcgca gtcaaatacg ccgatttaag caaaaaccgc accagcgatt atgtgttcga
     7681 ctgggacgcc atgctctcat ttgaaggcaa caccgccccc tacctgcaat acgcctacac
     7741 ccgcgtgcaa agcgtgttcc gcaaagccgg agaatgggac gcaaccgcac cgaccgtttt
     7801 gaccgaacca ctggaaaaac agcttgccgc cgagcttctg aaatttgaaa acgtgctgca
     7861 aagcgtggcg gacaccgcgt atccgcacta cctcgccgcc tacctttacc aagccgccac
     7921 cctgttcagc cgcttctacg aagcttgccc gattttgaaa gccgaaggcg caagccgcaa
     7981 cagccgcctg caactggcaa aacttaccgg caacacgctg aaacaaggct tggatttgct
     8041 gggcatcgat gtgttggacg taatgtaaac accgcaccgc ccgccatcct catccgaatc
     8101 tgaaaaaagc ggcgcgatac accgtatccg ccgcctttcc caaaatgcga aacaaacaaa
     8161 cgccaagcaa gcaagcaagc aagcaagcaa gcaaaaatta tagccccttt cctgccggcg
     8221 cacacacttt ccgcgcggca catccccctt tttccgcccc tcaaacccgc cttttcttca
     8281 ggcagggttt cagcccgcct cctttccctg ttttcctttc cccgacacgc gtgcgcgccc
     8341 ctgccgcacc gtgccgcgct tttgcgcccg gacggcatcg ttccgccatc tggttttctg
     8401 ttttaaatac cgctgtttca gaaagaaatg cagatgtttc gacacacagg acgacatata
     8461 aagtaccgcc ctatgtgtcg tcctgatttg gaaggggtta cacccctccc aaccagataa
     8521 agtctgatcc tgccgccccg aaggacggat gtccaagtgg cggggtttca accgaaaagg
     8581 aaatacgatg aatattcacg ccctgctctc caaacaatgg acgctgccgc cattcctgcc
     8641 gaaacggctg ctgctgtccc tgctgatact gctggtcccc aatgcggtgt tttgggtttt
     8701 ggcactgctg accgccaccg cccgcccgat tgtcaatttg gactaccttc ccgccgcgct
     8761 gctgatcgcc ctgccttggc gtttcgtcaa aattgccggc gtattggcgt tttggccggc
     8821 ggttttgttt gacgggctga tgatggtgat ccaactcttc ccttttatgg acctcatcgg
     8881 cgccatcaac ctcgtcccct tcatcctgac cgcccccgcc ccttatcaga taatgaccgg
     8941 gctgttgctg ctgtatatgc tggcgatgcc gtttgtgttg caaaaagccg ccgtcaaaac
     9001 cgacttccga cacattgccg tctgtgccgc cgttgtggcg gcagccggct atttcaccgg
     9061 ccatttgagt tactacgacc gggggcggat ggccaatatc ttcggcgcaa acaacttcta
     9121 ttacgccaaa agtcaggcga tgctctacac cgtcagccag aatgccgact ttattaccgc
     9181 cggcctggtc gaccccgtct tcctcccctt gggcaatcag cagcgtgccg ccacgcggct
     9241 gagtgagccg aaatctcaaa aaatcctctt tatcgtcgcc gaatcttggg ggctgccggg
     9301 caatcccgag cttcaaaacg ccacttttgc caaactgctg gcgcaaaaag accgtttttc
     9361 ggtttgggaa agcggcagtt ttcccttcat cggcgcgacg gtcgaaggcg aaatgcgcga
     9421 attgtgcgcc tacggcggtt tgcgcgggtt cgcactgcgc cgcgcacccg acgaaaaatt
     9481 tgcccgctgc ctccccaacc gtttgaaaca agaaggttac gccacctttg cgatgcacgg
     9541 cgcgggtagt tcgctttacg accgcttcag ctggtatccg agggcgggct ttcaaaaaat
     9601 caaaaccgcc gaaaacctga tcggtaaaaa aacctgcgcc attttcggcg gcgtgtgcga
     9661 cagcgagctg ttcggcgaag tgtcggcatt tttcaaaaaa cacgacaagg gactgtttta
     9721 ctggatgacg ctgaccagcc acgccgacta tcccgaatcc gacattttca accacaggct
     9781 caaatgcacc gaatacggcc tgcccgccga aaccgacctc tgccgcaatt tcagcctgca
     9841 cacccaattc ttcgaccaac tggcggattt gatccgacgc cccgaaatga aaggcacgga
     9901 agtcatcatc gtcggcgacc atccgccgcc cgtcggcaac ctcaatgaaa ccttccgcta
     9961 cctcaaacag ggacacgtcg cctggctgca cttcaaaatc aaataacaac aatgccgtct
    10021 gaacgcacca acagccttca gacggcattt tgcagacaga ccgacctcct caagcccact
    10081 tttttcatca tctccgataa attgctttgt aagaatccaa aaatccaaaa tcacaggaag
    10141 ttatcaaaaa aacagaaacc atccgccgtc gttcccgcga aagcgggaat ccagacccgg
    10201 aaaccgcaga aatttatcgg aaaaaccgaa actcgaaaac aacggacaga attcccgcct
    10261 gcgcaggaat gacggctcat acatcaccca aaaaaaattg aaaccaaaca gctgggattc
    10321 cctcttgcgt gaggctgaca gatgccgtct gaaagacttt cagacggcat agggcgtttt
    10381 ctctttgaat ttaaattttg ccacaaaaaa gccgccccaa atgaataccc gggcagcttt
    10441 ttgttgatat gactccaatc agcggtgttg cggattgtaa cgtttttcca aacgcaggaa
    10501 tatccagcct aagaaagtcg tcatcaacag ataaatcagg gcggcggtgt aaagcggttc
    10561 ttcataaacc gaataccggc ccgtaatcgt attctgaaca tacgccaact ccgccacagc
    10621 aatgaccgac agcagcgagc tgtctttcaa gagcgtgatg aactcgctcg ccaaaggcgg
    10681 cagcatacgg cgcaatgcct gcggcagaat cacatagcgc atcgcctgcg gataggtcag
    10741 tcccaaagaa cacgccgcct ccatctgtcc tttgtctata gactggatgc ccgcgcggaa
    10801 aatctcacag atatacgccc ccgagttggc gatcagtgcc aaagaaccgg caatcagcgg
    10861 cccgtatccg cgacgcagcg cgattgccgc ctcgccgctg accaaaatgc cgtctgaagg
    10921 atggacgaaa aacggaaacc acacatacgc ccaaatcaca atctgcacaa acagcggcgt
    10981 accccggaac agggtaacgt acagcagcga aaccttacgc aacgcccacg ccagcacgcg
    11041 catcggcgca ccggcttttt ccaagtgaat caggcgcgcc aacgccaaca acagacccaa
    11101 taccgaaccg cccgccgtcg ccacgaccgt cagccccaag gtcgtcagtg cgccgtaaag
    11161 aaacatccag cggtattcgt aaataatgtc aaaacgaaaa tccataaacc gtccgtatca
    11221 aaaaccggcg gaactgccgc cgttgcaaaa taatccgcca ttttaccgta aaaaccgccg
    11281 cctgaacttt tttatcgcgg cagacggcgg ttgcgcgtct ccgcaaaaat gcagggcgcg
    11341 cggttttcag acggcatttg ccgttcaagg ccgtgcggtg tctttaccaa atgcccaacc
    11401 attcgcccac ggaatccatc caatccttat tgcccccgcc gctcctgcct gcccggcggt
    11461 acgcccacgg cgcttgcgga tttttagctt tccacaatcc tttgcgttcc ctttccgcct
    11521 gaatttgagc gtcggcatag tcggcaaaat ccgccttatc ctgctgttct ttagcataac
    11581 ttttataatg ccacgccgcc ccgtcctgca cctgcatcag gttcaaatcg gttttgccgg
    11641 cggatacctg cgccacttcg cgctgatagc ggtcggtttc aaacacacgt acactgactt
    11701 tcctaccctc cgccgccgcg cgcaggttgt cgcgcgaacg tgtaccgtaa gcctgtttca
    11761 tctccggtgc gtcgatatac gccatccgaa ttttatgttt cgcgccgtcg ccgtcgatga
    11821 cgtgaagggt atcgccgtca tagactttgg acaccgtgcc tgtgtagctg tggccggatt
    11881 tcgccgatgc ccgtcggcga acgggcgcgt cggaacccac gtcccctgcc gtgccgagta
    11941 cgtcgagtac ggcaaccgcc gtccgcaccg cctcactgtc atatcccgta taacccaacg
    12001 cgcccaaaag cgacagggcg acgggaagcc atttcatgat ttttttaatc tgcatatttt
    12061 tcaaatgccg atgccgtctg aacatctctg aaccggattt cagacggcat cttaacgtca
    12121 ggattaccct tggcagggat agatgacttt cgcaccctct tccgtcccca aaatcaacac
    12181 atcggcggca tcgcgggcga atatgccgtt ttcgagtacg ccggtgattt tgttgatttc
    12241 atcttccatc gtcagcggct gatcgatatt caagccgtgg acatcgacga tttggttgcc
    12301 gtaaaacgtg gtgtagccga tacgcagttc gggctgtccg cccatggcaa gcagtttgcg
    12361 cgaaacaagg gagcgcgcgc tttcgacggc ttccacgggc agtgggaatt tgcccagccg
    12421 ggaaacatat ttgctttcgt ccgcaatgca gacgaatttt tctgatgcgc tggcgacgat
    12481 tttttcgttg aggtgcgcgc cgccgccgcc cttaatcatt tgcagggcgt ggttcacttc
    12541 gtccgcaccg tcgatataga ccgccaaacc ggacacttcg ttcagggaca cgacgggaat
    12601 gtcgtaccgg gcaagcagtt cgccggattt tttggaggtt gaaaccgcgc ctttgatttt
    12661 tttaccgctt ttgcccaagg cttcgatgaa gaaattgatg gtcgaaccgg taccgatgcc
    12721 gatgtattcg ttttcgggta cgaattcgac tgctttttcg gcggcgatgc gcttgagttc
    12781 gtcttgtgtc gtcatatttt tatcctttgg gaaaccgtat caacaaacag ccgccatctt
    12841 aacatttttt tgcacgtcct gcccgccgcg ttcaaatccg taccagcaat accgccgcct
    12901 gcgcctctat gccttccatc cgcccgagat agccgagttt ttcgttggtt ttgcctttga
    12961 tattgacgca cgaaatatcg atacccaaat cggcggcgat gttggcacgc atttgcggaa
    13021 tgtgcggtgc gagtttgggt ttctgtgcaa tcacggtcgt atcgacattg accgcctgcc
    13081 aaccctgcgc ctgaacgctt tgatacgccg cacgcaaaag gacgcggctg tccgcatctt
    13141 tgaactctgc ggcggtgtcg ggaaaatggc tgccgatgtc gcccaaacct gccgcaccga
    13201 gcagcgcgtc ggtaacggcg tgcagcagcg cgtcggcatc ggaatgcccg agcagccctt
    13261 tttcaaatgg gatttcaact ccgccaagta tcagctttct gccttcgatc aattggtgga
    13321 catcgtagcc ctgtccgata cggatattcg tcatcgtttg tgttcctgat gttttgaatt
    13381 gaatttcaga cggcattgag cagcagcctg acgatgtatg cgtcctgcgg ctgcgtcagt
    13441 ttcaaattgc gcgcgtcgcc ctgtatcagt agcggacgca cacccagttt ttccacggcg
    13501 gacgcttcat cggtaatgcc gtccaagttt tccgcagcca atgcgcggtg cagcaaaccc
    13561 gcttgaaaaa gctgcggcgt ttgcgcctgc caaagccccg aacggtcgac agttgcactg
    13621 atttgtccgc tttctgcgcg cttgagcgta tcggcaacgg gaactgccaa aatcccgcct
    13681 tcggcggcgt tgcccgcctg ttctatcaac cgcgtcagag cttcagacgg caggcagcag
    13741 cgggcggcat cgtgtaccag aatattgtcg gtttccgccg ccaaaccggt ttccaacagt
    13801 tttgccacac cgttgcggac agtttcggcg cgggtctgtc caccgttttt ccacacccga
    13861 acctgtggaa atgccgtctg aaccttatcg gcaaacgtgt cttcgggcga gacgacaacg
    13921 acggtcaaat cgacggcctc atgccgttca aaaatcccaa gtacatgttc taaaacggtt
    13981 ttgcttccga tttcgacata ttgcttgggt ttgtccgcac cgaaacgcac cccgatgccg
    14041 gcggcgggaa tcagcgcgat atttttgcgt ttcatgcgtt ttccccgccg ttttcagacg
    14101 gcacggcttc cttacgccaa atacaggctt cgcccaagcc gtcaagatat tcttcgtgcg
    14161 ccgccagttc ggcttcatcc gccttaatca ctttcagttt ggcaccgagt ttcatttcgg
    14221 cagatgcaac aggtttggct gtcggttctt cctcctcggt ctcccccatc aagtcgaatt
    14281 gccgccgcgt catcgccaga tagacttcgc ccaaaagttc gcagtcgatc aatgcgccgt
    14341 gcaggacgcg cttgctgcgg tcgacggaaa aacggttgca cagggcatcc agactggctt
    14401 tctgcccggg gaacatttcg cgcgccatcg ccagggtatc ggtaacggtg cagccgagtt
    14461 cctcgacggt cggcagcccc atccggcgga actccatatt gaggaagccc acatcgaatt
    14521 tggcattgtg gataatcagt tccgcaccgc gcagaaaatc ggcaatctgc ctgccgacct
    14581 ctgcaaacgg cggcgcgttt ttctcttcca aaacctgtat cgtcaagccg tggacgcgtg
    14641 ccgcctcttc gggcatatcg cgctcggggt ggacatagag gtgcaggttt ttgtcggtca
    14701 tttggcggtt gaccatttcc agaccggcaa actcgaccag acggtcgccg ccgtcggcat
    14761 acaggccggt ggtttcggta tcgaggatga tttggcgtgt catcatatcg gtgtctttct
    14821 tctgtcttcg taaattgatt attttttaag caatgtattt ttctgttttc atttcaatgc
    14881 acaaacccac ttattcacag tgtgttcaca acattgggca ggcggattgt gtattttggg
    14941 gacaattttt tcagacggca ttcaaggttt tttcctgatt gccgccgcgc ctaaaaaccg
    15001 cctttcgcgc ttaatcaaaa ataccgccaa cggaatattg cccaaggcga caatcagata
    15061 cagcagggaa atgctgtcaa acaaaaacag caataccgcg ctcaaaacgg cggcggaaac
    15121 cataaagatg ccgttaacga tattgttggc ggcaacggcg cgggcgcgga aagtctcgct
    15181 gctggcggtt tgcagccagg tatagagcgg aacggagaaa aatccgccga aaaagccgat
    15241 cagcgtcatc accgccatca cggagtatgc ccatccttgc gataaaaacc aaaaaatgcc
    15301 gttcagccct tcaaaacggt gtccgtgcgt cagccacacc aaaaccaggc cgcaaaccgt
    15361 caaacccaac gcaccaaccg ttacccaagc caacatcagc cgttccctgc cgaacttggc
    15421 acacagtacc gaaccggcgg caataccgat ggaaaacaaa gcaagcatca ggttaaaaac
    15481 attatcgttg ccgcccaaat ggatttgggt aaaggtcggc agttgcgtgg tataaaccgc
    15541 gccgacaaac caaaaccacg agatgccgat aatggcggta aaaacgggat tgtgccgcac
    15601 cgtttcacgc agcagggatt ttgtaccacg gacaatattc cactcgattt gggtatcggc
    15661 agccttggcg ggtacggacg gcataaacag gctgccggcc gttcctccga cggcgaccag
    15721 caaaaccagt atcccgacaa tataaggcgg cgcgccggca accgccgttc ccaaaatctg
    15781 accaaacagg atggcgacaa acgtaccgga ttcaatcagg ctgttgccca tcatcagctc
    15841 gttgtcgtcg agataatcgg gcagaatggc atatttaagc gggccgaaca gcgtcgattg
    15901 cgcgcccatg caaaacaggc agagcaagag cagcggggct gaccggatat aaaacccgta
    15961 tgccgccacc gccataatga tgatttccag caccttgacc caacgcgcca aaacggcctt
    16021 gtcgaatttg ttacccaact gccccgacag cgcggaaaac aggaaatacg gcaaaataaa
    16081 cagcagtgcg cccaagttca acatctgtcc ggcaggcaga acgccgtttt gccccaaacc
    16141 gtaaaacccg atcatcacaa acagcgcggt tttgaacaca ttgtcattga acgcgccgag
    16201 aaactgcgta gcgaaaagag gcccgaaacg gcggctttta accagtccca aaccgccttt
    16261 tttagcgtcc atcgttttcc ttctcttttt caatcagttt acttgtcgaa tcatcatcca
    16321 tcaaaatgcg gtgcgccggt ccttccaaat cgtcaaactg tccgtttttg cccgaccacc
    16381 agaaaaacca gccgatgaca aatgccaaaa taatgctgat gggcaccaga ataaacatac
    16441 tttccatcac atattcccta tcaaatcgtt caaaacaaaa gtctgccctg acacggtcag
    16501 atattcgtta cgcaaagttc cgacgggggc ttcgtcaaaa aacagctcga tacggtcttt
    16561 gaccacgcgc caatattggg gaatttccgt ctgaccgaac ggcgacagga catggttgcc
    16621 cgtcccatct tccaatttaa cggcaaaacg cccgctctgc agccgtgctt ccgattcgtc
    16681 gtcggcaaga aggatgaaaa agcctatatg ccgtcccgat tggtcatgaa tactgaaata
    16741 atgcataaat tccccacccg ccttttttca gacggcatca actaaaaaca gggcgaatgt
    16801 accagtttgg acgggaaaaa tgcaaagaaa ttctccctcc cccagccgaa aacaccggca
    16861 aaccgcatat cccccttttt tccgtcaaaa tacccgactt ccgccatttt cacgcaaacg
    16921 cccgattaag ccaagcaatt gcaaagattt tttgctagaa tagcctgctt cttttatcaa
    16981 ccgtttcaga cggctccact actttcccgt caaggaaagc aaagcggatt cggcacgaat
    17041 ccgattagta tccgtgtccg attccaatgc cgtctgaaac tttccggagt aagaaaatgt
    17101 cccaaaaatt gatcttggtt ttgaactgcg gcagctcgtc cctcaaaggc gcggtcctgg
    17161 ataacggcag cggcgaagtc ctgctgagct gccttgccga aaaactcaac ctgcccgatg
    17221 cctacattac attcaaagta aacggcgaaa aacacaaagt cgatctgtcc gcacaccccg
    17281 accacaccgg cgcggtcgaa gccctgatgg aagaactcga agcccacggc ctcgacagcc
    17341 gcatcggcgc catcggccac cgcgtcgtca gcggcggcga actgtacaac gaatccatcc
    17401 tcgttgacga cgaagtcatc gccggcatcg aaaaatgcat cccgctggct cctctgcaca
    17461 atcccgccca cctcttgggc ctgcgtgccg cgcaaagcat tttcaaaggc ctgcccaacg
    17521 tcgtcgtatt cgatacctcc ttccaccaaa ccatgcccga agtcgcctac aaatacgccg
    17581 ttccgcagga gttgtatgaa aaatacggcc tgcgccgcta cggcgcgcac ggtaccagct
    17641 accgcttcgt tgccgacgaa accgcgcact tcctcggcaa agacaaaaaa gacctgcgta
    17701 tggtcattgc ccacttgggc aacggcgcat ccatcaccgc cgtcgccaac ggagaatcgc
    17761 gcgacaccag tatgggcctg accccgctgg aagggctggt aatgggtacg cgcagcggcg
    17821 acatcgatcc ttccgtattc ggcttcctcg ccgaaaacgc caatatgacc attgcccaaa
    17881 tcaccgacat gctgaacaaa aaatccggcc tgctcggcat ttccggcctg tccaacgact
    17941 gccgcaccat tgaagaagaa gccgccaagg ggcataaagg cgcgaaattg gccttggata
    18001 tgtttatcta ccgccttgcc aaatacatcg gcagtatggc ggttgccgca ggcggcttgg
    18061 acgcactggt ctttaccggc ggtatcggcg aaaactccga catcatccgc gaacgcgtaa
    18121 tcggctactt gggcttcctc ggtctgaaca tcgaccaaga agccaacctg aaagcccgct
    18181 tcggcaacgc cggcgtgatt accactgccg acagcaaagc cgttgccgtg gtcattccga
    18241 ccaacgaaga gctgatgatt gcccacgata ccgcccgttt gagcggtctg taaggtttta
    18301 tcctcacgcg aactgcctcc ggaaacggag gcagtttttt tatagtggat taaatttaaa
    18361 cc
//
LOCUS       DAXDZG010000044        18339 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXDZG010000044 DAXDZG010000000
VERSION     DAXDZG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.044"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            6..81
                     /locus_tag="ACFB78_001295"
     tRNA            6..81
                     /locus_tag="ACFB78_001295"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="ACFB78_001296"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="ACFB78_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC1269276.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="ACFB78_001297"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="ACFB78_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC1269277.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="ACFB78_001298"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="ACFB78_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC1269278.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="ACFB78_001299"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="ACFB78_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC1269279.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFB78_001300"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFB78_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC1269280.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARSLAALAEKK
                     AGEEAA"
     gene            3028..3399
                     /gene="rplL"
                     /locus_tag="ACFB78_001301"
     CDS             3028..3399
                     /gene="rplL"
                     /locus_tag="ACFB78_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC1269281.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3590..7768
                     /gene="rpoB"
                     /locus_tag="ACFB78_001302"
     CDS             3590..7768
                     /gene="rpoB"
                     /locus_tag="ACFB78_001302"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC1269282.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7733..7873)
                     /locus_tag="ACFB78_001303"
     CDS             complement(7733..7873)
                     /locus_tag="ACFB78_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269283.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7921..12096
                     /gene="rpoC"
                     /locus_tag="ACFB78_001304"
     CDS             7921..12096
                     /gene="rpoC"
                     /locus_tag="ACFB78_001304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC1269284.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12385..13455
                     /locus_tag="ACFB78_001305"
     CDS             12385..13455
                     /locus_tag="ACFB78_001305"
                     /inference="COORDINATES: protein motif:HMM:NF020373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFC1269285.1"
                     /translation="MKVSQSAMNKLVIAVIAVIASMGLTACAGGGSDSSMSVQPSVSE
                     QLKDNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGE
                     SVKAMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGELDVLPKGLSEQKIEFYGADK
                     EQNYALLKTWVYEQPYSVVRGYFGYSRKDGNPIEEDGQNPEEIPFDLYLGDIRGVATD
                     EDKLPKAGSFQYEGRAFGGNGVLSKESLDNHNGVFRYTIDFDRRKGSGSIEGMEQYGK
                     IKLEEAAIERIPYRESGSSLGLKDRVSYFGVNEGVAMLEKDNEIKKYHLGIFGEAANE
                     VAGAVSQEHKHQAVIGFGGEKK"
     gene            13693..13935
                     /locus_tag="ACFB78_001306"
     CDS             13693..13935
                     /locus_tag="ACFB78_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269286.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            14132..14992
                     /locus_tag="ACFB78_001307"
                     /pseudo
     CDS             14132..14992
                     /locus_tag="ACFB78_001307"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            15171..15542
                     /gene="rpsL"
                     /locus_tag="ACFB78_001308"
     CDS             15171..15542
                     /gene="rpsL"
                     /locus_tag="ACFB78_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC1269287.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15660..16130
                     /gene="rpsG"
                     /locus_tag="ACFB78_001309"
     CDS             15660..16130
                     /gene="rpsG"
                     /locus_tag="ACFB78_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC1269288.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16149..18254
                     /gene="fusA"
                     /locus_tag="ACFB78_001310"
     CDS             16149..18254
                     /gene="fusA"
                     /locus_tag="ACFB78_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC1269289.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4950 a         3969 c         4759 g         4661 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggcc tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgataaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcagtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaatagcatg gctattacta aagaagacat tttggaagca
     3061 gttggttctt tgaccgtaat ggaattgaat gacctggtta aagcttttga agaaaaattc
     3121 ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg gtgccggtgc tgccgatgct
     3181 gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg gcgatcaaaa agtcggcgtg
     3241 attaaagttg tccgtgcaat tactggtttg ggtctgaaag aagctaaaga catcgttgac
     3301 ggcgcaccta aaaccattaa agagggtgtt tctaaagctg aagccgaaga catccaaaaa
     3361 caactggaag cagcaggcgc taaagtcgaa atcaaataat ttgatgcttc ttatgaaggc
     3421 tggcagtttt ctgccagcct tattttgctt cttaaaataa gcattaagta ttgtttacat
     3481 ttatttgcat agtttttatc aaatcattgc aaataaatgt aaatatcaga ttgatgcgta
     3541 ccgttgtttt cagacggcct attattgaaa attacttttc ggagtgtgta tgaactattc
     3601 gtttaccgag aaaaaacgta tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt
     3661 tccttttcta ctggcaaccc aaattgattc ttatgcgaaa tttttgcagc tggaaaatgc
     3721 ttttgacaaa cgtaccgatg acggtctgca agcggcattt aattctattt tcccgattgt
     3781 gagccacaac ggttatgctc gtctggagtt tgtatattac acgctgggcg agcctttgtt
     3841 cgatatccct gaatgtcaat tgcgcggaat cacttatgct gcccctctgc gcgcgcgtat
     3901 ccgtttggtg attttggata aggaagcgtc taaaccgacg gtaaaagaag ttcgtgaaaa
     3961 cgaagtgtat atgggcgaaa ttccgttgat gaccccgagc ggttcttttg tgattaacgg
     4021 cacagagcgt gtgattgttt cccagttgca ccgttcgccc ggtgtgttct tcgagcatga
     4081 caaaggtaag acacactctt ccggcaaatt gttattctcc gcccgcatca ttccctaccg
     4141 tggttcatgg ttggattttg aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg
     4201 ccgccgtaaa atgccggtaa cgattttgtt gaaggctttg ggctacaaca atgagcaaat
     4261 cttggatatt ttctacgaca aagaaacgtt ttatttgtct tcaaacggtg ttcaaaccga
     4321 tttggtcgca ggccgtctga aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg
     4381 caatgtattg gttgccaaag gtaagcgcat tactgcgaaa aatatccgtg atattaccaa
     4441 tgcaggcctg acccgtttgg atgtagaaca ggaaagcctg ctgggcaaag cattggctgc
     4501 cgatttgatt gattcggaaa ccggcgaggt attggcttct gccaatgatg aaattacaga
     4561 agagttgttg gccaaatttg atattaacgg cgtaaaagaa attacgaccc tttacatcaa
     4621 tgagttggat cagggtgctt atatttccaa taccttgcgc acggatgaga ctgccggccg
     4681 gcaggcggcg cgtgttgcga tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga
     4741 ggcggtcgag caattgttta accgcttgtt cttcagtgaa gacagctacg atttgtcccg
     4801 cgtaggccgt atgaaattta atacgcgcac atacgaacaa aaactgtccg aagcccaaca
     4861 aaactcttgg tacggccgcc tgttgaacga gacgtttgcc ggtgctgccg acaaaggcgg
     4921 ttatgtcctg agcgtcgaag atattgtcgc ctcgattgcg actttggtcg agttgcgtaa
     4981 cggccatggc gaagtggacg atatcgatca cttgggcaac cgtcgagtac ggtcggtagg
     5041 cgagctgact gaaaaccaat tccgcagcgg tttggctcgt gtggaacgtg ccgtaaaaga
     5101 acgtttgaat caggcggaat cagaaaactt gatgccgcac gacttgatta atgcgaaacc
     5161 cgtttctgcc gccatcaaag aattcttcgg ctccagccaa ttgagtcagt ttatggatca
     5221 gaccaatccc ttgtctgaag taacccataa acgccgtgta tctgcattgg gcccgggcgg
     5281 tttgacccgc gaacgtgccg gcttcgaagt gcgggacgtg catccgaccc actatggacg
     5341 tgtatgtcct attgaaacgc ctgaaggtcc gaacatcggt ttgatcaact cattgtctgt
     5401 ttacgcgcgc accaatgatt acggtttctt ggaaacgcct taccgccgcg ttattgacgg
     5461 caaagtaacc gaggaaattg attacttgtc tgccatcgaa gaaggccgct atgtgattgc
     5521 acaggcgaat gccgatttgg attcagacgg caatctgatt ggcgatttgg ttacctgtcg
     5581 tgaaaaaggc gaaaccatta tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac
     5641 cggtcaggtg gtatccgttg cggcatccct gattccgttc ttggaacacg atgacgcgaa
     5701 ccgcgcattg atgggtgcca acatgcaacg tcaggcagtg ccttgtctgc gtcctgaaaa
     5761 accgatggtc ggtaccggta tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt
     5821 tgcccgccga ggcggcgtgg tcgagtatgt cgatgccaac cgcgttgtga tccgtgtcca
     5881 tgacgacgaa gcgactgccg gcgaagtggg tgtcgatatt tacaatttgg ttaaattcac
     5941 ccgttccaat caatctacca acatcaacca acgtccggct gtcaaagcag gcgatgtttt
     6001 gcaacgcggc gatttggtgg ccgacggcgc gtccaccgat ttgggcgaat tggctttggg
     6061 tcaaaatatg accatcgcct tcatgccgtg gaacggttac aactacgaag actcgattct
     6121 gatttccgaa aaagtggctg cggacgaccg ctatacttcg attcacattg aggaattgaa
     6181 tgtcgttgcc cgcgatacca agctgggtgc ggaagacatt acccgcgata ttccgaactt
     6241 gtccgagcgt atgcaaaacc gtttggacga atccggtatc gtttacatcg gtgcggaagt
     6301 agaagccggc gatgtgttgg taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc
     6361 ggaagaaaaa ctgctgcgcg ccatcttcgg cgaaaaagcg tctgacgtaa aagatacttc
     6421 attgcgtatg cctaccggta tgagcggtac ggttatcgac gttcaagtct ttacccgcga
     6481 aggcatccaa cgcgacaaac gcgcccaatc cattatcgat tccgaattga aacgctaccg
     6541 tttggatttg aacgaccaat tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg
     6601 tatgattgtc ggtcagaaag ccaacggcgg tccgatgaag ctggccaaag gcagcgaaat
     6661 cacgaccgaa tatctggcgg gtctgccgag caggcacgat tggttcgata tccgtttgac
     6721 cgatgaagat ttggccaagc agttggaact gattaaattg agcctgcaac aaaaacgcga
     6781 agaagcggat gagttgtatg agatcaagaa gaaaaaactg acccaaggcg acgagctgca
     6841 acccggcgta caaaaaatgg tgaaagtctt tatcgccatc aaacgccgtc tgcaagccgg
     6901 tgacaaaatg gcgggccgcc acggtaataa aggtgtggta tctcgcattc tgcctgtgga
     6961 agacatgccg tacatggcgg acggccgtcc tgtggacatc gtactgaacc cgttgggcgt
     7021 accttcccgt atgaacatcg gtcagatttt ggaagttcac ttgggttggg cggcaaaagg
     7081 tatcggcgag cgcatcgacc gtatgctgaa agagcgacgc aaagccggcg agctgcgcga
     7141 gttcttgaac aaactctaca acggcagcgg taagaaagaa gatttggaca gcctgactga
     7201 tgaagaaatc atcgaattgg cttccaatct gcgtaaaggt gcatctttcg cctctcctgt
     7261 attcgacggt gcgaaagagt ctgaaatccg cgaaatgttg aacttggctt acccaagcga
     7321 agatcctgag gttgagaaac tgggcttcaa cgacagtaaa actcaaatca cgctgtatga
     7381 cggccgttca ggcgaagcat ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa
     7441 actgcaccac ttggttgacg aaaaaatgca cgcgcgttct accggcccgt acagtctggt
     7501 tactcaacaa cctctgggcg gtaaagctca gtttggcggc caacgtttcg gtgagatgga
     7561 ggtttgggca ttggaagcat acggcgcggc atacacgctg caagagatgc tgactgtgaa
     7621 gtctgacgac gtgaacggcc gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa
     7681 aatcgatgcc ggtatgcccg agtccttcaa cgtattggtc aaagagattc gctcactggg
     7741 tttggatatc gatttggaac gctactgata cgggtttcag acggcataag gggagctgtt
     7801 ctgcaggtat gcggggcagc ccgacaatgt ttaaaaacga aatgccgtct gaaagcactg
     7861 tacctttatc catatcgaaa atccgccacg cggtaaaaat atttccttca aggagcagaa
     7921 atgaatttgt tgaacttatt taatccgttg caaaccgcag gtatggaaga agagtttgat
     7981 gccatcaaaa tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtt
     8041 aaaaaacctg aaaccatcaa ctaccgtacg ttcaaacccg agcgcgacgg cttgttctgc
     8101 gccaaaatct ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgattg
     8161 aaatttaaag gcgtaacctg tgaaaaatgt ggcgtagaag ttaccctgtc caaagtgcgc
     8221 cgcgaacgca tgggccatat cgaattggct gcacccgttg cccatatttg gttcttgaaa
     8281 tccctgcctt cccgcttggg tatggtattg aacatgacgt tgcgtgatat cgaacgcgta
     8341 ttgtactttg aagcattcgt ggtgaccgat cccggcatga ctccgttgca acgtcgtcaa
     8401 ttgctgactg aagacgatta ctacaacaaa ctggacgaat acggcgacga cttcgatgcc
     8461 aaaatgggtg cggaaggtat ccgcgaattg ttgcgaacct tggatgtagc aggcgaaatc
     8521 gaaatcctgc gccaagagct tgaatcgacc ggttctgaca ccaaaatcaa aaaaatcgct
     8581 aaacgcttga aagtattgga agccttccat cgttccggta tgaagctgga gtggatgatt
     8641 atggatgtgc tgccggtatt gccgcctgat ttgcgtccgt tggttccgtt ggacggcggc
     8701 cgttttgcca cttccgattt gaacgatttg taccgccgcg tcatcaaccg caacaaccgt
     8761 ctgaaacgcc tgctggaact gcatgcgcct gacatcatcg tccgcaacga aaaacgtatg
     8821 ttgcaagaag cggttgattc gctgttggat aacggccgtc gcggtaaagc catgaccgga
     8881 gccaacaaac gtccgctgaa atcgttggca gatatgatta aaggtaaagg cggccgcttc
     8941 cgtcaaaacc tgttgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc
     9001 ccatacctgc gtctgcacca atgcggtttg ccgaaaaaaa tggcgttgga actgttcaaa
     9061 ccatttattt tccacaaatt ggaaaaacaa ggtttggcgt ctaccgttaa agctgcgaaa
     9121 aaattggtag aacaagaagt acctgaagta tgggatatct tggaagaagt catccgcgaa
     9181 catccgatta tgttgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgag
     9241 cctattctga tcgaaggtaa agcgattcag ttgcacccat tggtgtgtgc cgcatttaat
     9301 gccgactttg acggtgacca aatggcagta cacgttccat tgagcttgga agcgcaaatg
     9361 gaagcacgca cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg
     9421 attatcgtac cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc
     9481 aatgccaaag gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat
     9541 accaaacagg tcgagctggg tacaaaaatc accgtacgtc tgcgcgaatg ggtgaaaaac
     9601 gaagcaggtg agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg
     9661 ttgagcgaaa tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa
     9721 aaagaaattt ccaagctgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt
     9781 atcttcgccg accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc
     9841 attgccgttg acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat
     9901 gccgaagtta aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc
     9961 tacaacaaag tggtcgatat ttggggtcgt gccggcgata agattgctaa agcgatgatg
    10021 gacaacctgt ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca
    10081 ttcaactcca tttatatgat ggcggactcc ggtgcccgtg gttctgcggc tcagattaaa
    10141 cagttgtccg gtatgcgcgg tttgatggcg aaacctgacg gatcgattat tgaaacgccg
    10201 attacctcaa acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt
    10261 gcgcgtaagg gtttggcgga taccgcgttg aaaaccgcga actccggtta cctgacccgt
    10321 cgtctggtgg atgtaactca agacttggtc gttgttgaag acgattgcgg tacttcagac
    10381 ggctttgtca tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt
    10441 attttgggtc gtgttaccgc gtctgacgtt gtcgatccgt caagcggcga gactttggtt
    10501 gaagccggta cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat
    10561 gaagtcaaag tccgtactcc gattacttgt aaaacccgcc acggtctgtg cgcgcactgt
    10621 tacggccgtg acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt
    10681 gcggctcagt ctattggtga accgggtacc cagttgacca tgcgtacgtt ccacatcggt
    10741 ggtgcggcat cccgtgcggc agcagccagc caagtcgaag ccaaatccaa cggtacggca
    10801 cgtttcagca gccaaatgcg ttatgttgcc aacaacaaag gcgagttggt tgtcatcggc
    10861 cgttcttgcg aagtcgtgat tcacgatgac atcggccgtg aacgcgaacg ccacaaagta
    10921 ccttacggtg ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg
    10981 gcaacttggg atccgcatac ccgtccgatg attaccgaac atgcaggtat ggtgaaattc
    11041 gagaacatgg aagagggcgt taccgttgca aaacaaaccg atgatgtaac cggtttgtcc
    11101 gctttggtag tgattgacgg taaacgccgt tcctctagtg cttccaaact gctgcgtccg
    11161 actgtgaaac tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg
    11221 gtttcgatgg cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt
    11281 aaaggcgacg tattggcgcg tattccgcaa gcctcttcca aaacccgcga cattaccggc
    11341 ggtctgccgc gcgttgccga gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg
    11401 gcggaaatta ccggtaccgt ttccttcggt aaggagacca aaggcaaaca acgtctgatt
    11461 atcactgacg tggacggtgt agcatacgag actttgattt ccaaagagaa acaaattctg
    11521 gtacatgacg gtcaagtggt aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg
    11581 cacgatattc tgcgtctgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg
    11641 caagaggttt accgcctgca aggtgtgaag atttccgata aacacatcga agtcatcatc
    11701 cgtcaaatgt tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt tattaccgga
    11761 gagcaggtcg aacgcggcga tgtgatggcg gccaatgaaa aagctttgga agaaggcaaa
    11821 gagccggcac gttacgaaaa catattgctg ggtattacca aagcctccct gtccaccgac
    11881 agcttcattt ctgccgcatc gttccaagaa accacccgtg tgttgaccga agctgccatc
    11941 atgggcaaac aagacgaact gcgcggtctg aaagagaatg tcatcgtcgg tcgcctgatt
    12001 cctgccggta ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag
    12061 caggagactg ctgaaaccca agtaacggat gaataatctt cggcgcgttc attcaataaa
    12121 aaaccgcaag ccttgagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt
    12181 tcgtcatttt gaggcgtgtg gatataagcc cgataagtgt cccatatctt cgtgccgtca
    12241 aattatgcat cgaatagaac gagttaaatc cgaaggttat tgtgttaaat attgcaaaaa
    12301 tactttaaaa atttcgcacg gtgggggggt agaattccca gacttaaagc ttaagttttt
    12361 ttaacaaagt ttgggggagt ctaaatgaaa gtatctcaat cagcaatgaa taaacttgtt
    12421 attgctgtta ttgctgttat tgcttctatg gggctaacag cgtgcgcggg tggcggttcc
    12481 gattcatcta tgtcggttca accaagcgtt tcggaacaac tgaaagacaa tgcaaatgtt
    12541 gatgcgaaag atgaaaaagt tattgaatat ctgaagaaat catcattaaa agatgtacca
    12601 aaagaattgc aagccaaagt attaaaggtt aaaggtgatg aatatacagg agtgcgaaaa
    12661 caatatgcag gtaagctcgg taagggtgaa tctgtcaagg caatgctatt tctagacggc
    12721 gaagagccgt ttagtaagga acaacttcag aagatggatg tatatgttaa tggtaaaaaa
    12781 tacgaaggta gtaagggggg ggaactagat gttctcccca aaggtttgag tgagcaaaag
    12841 attgagtttt acggtgcgga taaagaacag aattatgcgc tattaaaaac ttgggtttat
    12901 gagcaaccgt attctgtggt tagaggctat tttggctaca gtcgaaaaga cggtaatcct
    12961 atagaagagg atggacaaaa tcctgaagag attccttttg acttgtatct gggcgacatt
    13021 agaggcgtgg caacagatga agacaaatta cccaaggctg ggagctttca atatgaaggt
    13081 cgggcatttg gcggtaatgg ggttttatcc aaagaatcgt tagataacca taatggcgta
    13141 tttagataca caattgattt tgatcgtaga aaaggctctg gctcaattga aggaatggaa
    13201 caatatggca agatcaaatt agaagaagct gctattgaga gaatccctta tcgagagtcc
    13261 ggctccagtt tggggttgaa agaccgggtt tcttatttcg gcgtgaacga aggggtggct
    13321 atgttagaga aggataatga gattaaaaag tatcatttgg gtattttcgg cgaagcggct
    13381 aatgaagtag caggtgctgt aagccaagag cataaacatc aagctgttat cggtttcggc
    13441 ggcgagaaga aatagagaaa gcactatttc tatttagtgc cgtttgtccg ggcaagatgc
    13501 cgtctgaagt ttcagacggc attttcttca aaaaacagtc cgccaaatcg aatactgcca
    13561 agcggcaatg caatacctca aaacagcaat aatttctttt aaaacagttg attcttactg
    13621 aacataaggc cgggcaatac gaaaagaaaa atggcgttgc tgggcatttt atttgaattt
    13681 gttatgaaat tgatgaaatc taaaaatatc ttttttatgg gatacctaat cgcttcggcg
    13741 acgtttgccg aggatatcgg cgtgcctgtc gaaccgatta acgtcggtag tcgggctgcg
    13801 atgccgtctg aaggggaaag cctcgccctc ctgccgtttg cagaggatgt gccgccggtt
    13861 cgcgatgcaa tgccgtctga agttcctaaa agcgcggcag gcggcgatgt tcgaggcggc
    13921 cgatctatac cgtgagttgt tgtcggaaag acccgatctt gtttatcccc gttttgattt
    13981 gggcgtgatg ctgtttgaag acaaacaata tcgtgaggca ttggttcagt tgcaccgggc
    14041 cgaggaggtg ttgccgcccg atatgcggca gcttgcacgg gaatatatcc gtcaggcgga
    14101 agcggtgcag gcgtggcatc cttcgttcaa tatgaactat gaacaaacag ataatgtaaa
    14161 caatgcgtcc ctttcacggg acatcgtcat caacggccgg aaatggataa aatcggaaga
    14221 cagcctgccc aaacgcgcca acgggataag gtacgaactg ggcgttgatc ggatgtttaa
    14281 catggcaggc aatcactttg cccgcttagg catcagcggt tccggtgtgc attattggaa
    14341 tgcccgggat tttagcgaac aggcgtttca tgccgaagta ggctaccgtt atcggaatag
    14401 ccgtctagag tggggattcc ggccctttgt caaacaaaac cggctgggca ataaccgtta
    14461 tacggcaaat acgggtattg tgttggatta cagccgccgt ctgaatgaaa aatggcgcag
    14521 cacccaatct ttccagtatg gccgcaaaca ataccatgac gaatatttgg caaaacgtta
    14581 caacagtaaa acgatttcgg tgagcggtac gttcagctat tatgctatgt cggcttggca
    14641 gttgtacggg ggtattagcg gtatgtttga taatactgtg gagaaagagc aggcttcacg
    14701 gcgttatggt gtaagtttgg gtacggtgaa aatattagac ggcggtcttg gactgaagtt
    14761 gggtgcgggt tacacgaaac ggatatttaa agcccctgca acattaattt acaattttac
    14821 tcggcgtgat gattaacatc ggatgagcgc ggctttatgg cataaaaaac tgtcgtggaa
    14881 aggatttaca ccccaaataa atttccgtta caacaagaac aacagcaata tgcccgcctt
    14941 ttattcgcac agcggcaagg gatggtttgt cagtatggaa aaaacgtatt gacagtattt
    15001 tctccagccg tccgactgat tgtgagggat gtcggtaaat atttatcggc aaacaagaaa
    15061 atcatctttt ttcttgtcgt tatgcttgac tgtctgcttg caataaaaat ataattccgc
    15121 tcttgccgac atggtgtcgg caaatattta actcaacagg acgagaaaat atgccaacta
    15181 tcaaccaatt ggtacgcaaa ggccgtcaaa agcccgtgta cgtaaacaaa gtgcccgcac
    15241 tggaagcctg cccgcaaaaa cgcggcgtgt gcacccgtgt atacacgact acccctaaaa
    15301 aacctaactc tgcattgcgt aaagtatgta aagtccgcct gaccaacggt tttgaagtca
    15361 tttcatatat cggcggtgaa ggccacaacc tgcaagagca cagcgtcgta ctgattcgcg
    15421 gcggccgtgt aaaagacttg ccgggtgtac gttaccacac tgtacgcggt tctttggata
    15481 ctgcaggtgt taaagaccgc aaacaagccc gttctaaata cggtgctaag cgtcctaaat
    15541 aattaccggg actcaaatag gcacgtcggc cgcctaagct gaacaacggc cgagtaagtg
    15601 aatactcaat tgggtattca tgggaataga cccgactgaa tagattaaag gaaattaaaa
    15661 tgccaagacg tagagaagtc cccaagcgcg acgtactgcc agatcctaaa ttcggtagcg
    15721 tcgagttgac caaattcatg aacgtattga tgattgacgg taaaaaatcc gttgccgagc
    15781 gtatcgttta cggtgcgttg gaacagattg agaaaaaaac cggcaaagca gcaatcgaag
    15841 tatttaacga agccattgca aactccaaac ctatcgtgga agtgaaaagc cgccgtgtag
    15901 gtggtgcaaa ctaccaagtt cctgttgaag ttcgtccttc acgccgtctg gctttggcaa
    15961 tgcgttgggt tcgcgacgcg gcccgcaaac gtggtgagaa atccatggat ctgcgtttgg
    16021 caggcgagtt gattgatgcg tccgaaggcc gtggcggtgc gttgaaaaaa cgtgaagaag
    16081 tacaccgtat ggctgaagcc aacaaagcat tctctcactt ccgtttctaa ttttgaaagg
    16141 ctaataaaat ggctcgtaag accccgatca gcctgtaccg caacatcggt atttccgccc
    16201 atatcgatgc gggtaaaacc acgacgacag aacgtatttt gttctatacc ggtttgaccc
    16261 acaagctggg cgaagtgcat gacggtgcgg ctactaccga ctacatggaa caagagcaag
    16321 agcgcggtat taccattacc tccgctgccg ttacttccta ctggtccggt atggcgaaac
    16381 aattccccga gcaccgcttc aacatcatcg acaccccggg gcacgttgac tttaccgtag
    16441 aggtagagcg ttctatgcgt gtattggacg gcgcggtaat ggtttactgt gcggtgggcg
    16501 gtgttcaacc gcaatctgaa accgtatggc ggcaagccaa caaataccaa gttccgcgct
    16561 tggcgtttgt caataaaatg gaccgccaag gtgccaactt cttccgcgtt gtcgagcaaa
    16621 tgaaaacccg tttgcgcgca aaccccgtac ctatcgtcat tccggtaggc gcggaagaca
    16681 gttttaccgg tgttgtcgat ttgctgaaaa tgaaatctat catctggaat gaagccgata
    16741 aaggtacaac ctttacctat ggcgatattc ctgccgaatt ggtcgaaact gccgaagaat
    16801 ggcgtcaaaa tatgattgaa gccgcagccg aagccagcga agaactgatg gacaaatact
    16861 tgggcggtga agatctggcc gaagaagaaa tcgtaggcgc gttgcgtcaa cgtactttgg
    16921 caggcgaaat tcagcctatg ctgtgcggtt ctgcatttaa aaacaaaggt gttcaacgta
    16981 tgttggacgc agttgtagaa ttgctgccag ctcctaccga tattcctccg gttcaaggtg
    17041 ttaatcctaa cactgaagaa gccgacagcc gtcaagccag cgatgaagag aaattctctg
    17101 cattggcatt caaaatgttg aacgacaaat acgtcggtca gctgaccttt atccgcgttt
    17161 actcaggcgt agtaaaatcc ggcgataccg tactgaattc tgtaaaaggc actcgcgaac
    17221 gtatcggtcg tttggtgcaa atgactgccg cagaccgtac tgaaatcgaa gaagtacgcg
    17281 ctggcgacat cgcagccgct atcggtctga aagacgttac taccggtgaa accttgtgtg
    17341 cggaaagcgc gccgattatc ttggaacgta tggaattccc cgagccggta atccatattg
    17401 ccgttgagcc gaaaaccaaa gccgaccaag agaaaatggg tatcgccctg aaccgcttgg
    17461 ctaaagaaga cccttctttc cgcgttcgta cagacgaaga atccggtcaa accattattt
    17521 ccggtatggg tgagctgcac ttggaaatta ttgttgaccg tatgaaacgc gaattcggtg
    17581 tggaagcaaa tatcggtgca cctcaagtgg cttaccgtga aactatccgc aaagccgtta
    17641 aagctgaata caaacatgca aaacaatccg gtggtaaagg tcaatacggt cacgttgtga
    17701 ttgaaatgga acctatggaa ccgggtggtg aaggttacga gtttatcgat gaaattaaag
    17761 gtggtgtgat tcctcgcgaa tttattccgt ctgtcgataa aggtatccgc gatacgttgc
    17821 ctaacggtat cgttgccggc tatcctgtag ttgacgtacg tatccgtctg gtattcggtt
    17881 cttaccatga tgtcgactct tcccaattgg catttgaatt ggctgcttct caagcgttta
    17941 aagaaggtat gcgtcaagca tctcctgccc tgcttgagcc gattatggca gttgaagtgg
    18001 aaactccgga agaatacatg ggcgacgtaa tgggcgactt gaaccgccgt cgcggtgttg
    18061 tattgggtat ggatgatgac ggtatcggcg gtaaaaaagt ccgtgccgaa gtacctctgg
    18121 cagaaatgtt cggttactcg accgacctgc gttctgcaac ccaaggccgc gctacttact
    18181 ctatggagtt caagaaatat tctgaagctc ctgcccacat agctgctgct gtaactgaag
    18241 cccgtaaagg ctaatcaggc aaataggccg tctgaaaggc tgaaatgatt tttcagatgg
    18301 cattgttctt taatcgatct ttaatgtaaa ggaattagc
//
LOCUS       DAXDZG010000045        18197 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXDZG010000045 DAXDZG010000000
VERSION     DAXDZG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18197
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.045"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            275..973
                     /locus_tag="ACFB78_001311"
     CDS             275..973
                     /locus_tag="ACFB78_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC1269290.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB78_001312"
     CDS             complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB78_001312"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC1269291.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="ACFB78_001313"
     CDS             2104..4509
                     /locus_tag="ACFB78_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC1269292.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSHDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="ACFB78_001314"
     CDS             4611..5612
                     /locus_tag="ACFB78_001314"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1269293.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6090..7970)
                     /locus_tag="ACFB78_001315"
     CDS             complement(6090..7970)
                     /locus_tag="ACFB78_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC1269294.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGLFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8126..8758
                     /locus_tag="ACFB78_001316"
     CDS             8126..8758
                     /locus_tag="ACFB78_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC1269295.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQNHT
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8830..10200
                     /gene="purB"
                     /locus_tag="ACFB78_001317"
     CDS             8830..10200
                     /gene="purB"
                     /locus_tag="ACFB78_001317"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC1269296.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTDMAHEL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFSRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10280..12149
                     /locus_tag="ACFB78_001318"
                     /pseudo
     CDS             10280..12149
                     /locus_tag="ACFB78_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(12285..12899)
                     /locus_tag="ACFB78_001319"
     CDS             complement(12285..12899)
                     /locus_tag="ACFB78_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC1269297.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALELDDNLVESLERQLGI"
     gene            complement(12935..15085)
                     /gene="dinG"
                     /locus_tag="ACFB78_001320"
     CDS             complement(12935..15085)
                     /gene="dinG"
                     /locus_tag="ACFB78_001320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC1269298.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPDFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15266..16564)
                     /locus_tag="ACFB78_001321"
     CDS             complement(15266..16564)
                     /locus_tag="ACFB78_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC1269299.1"
                     /translation="MPSETALPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGICIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16554..17465)
                     /locus_tag="ACFB78_001322"
     CDS             complement(16554..17465)
                     /locus_tag="ACFB78_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC1269300.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17573..17971
                     /locus_tag="ACFB78_001323"
     CDS             17573..17971
                     /locus_tag="ACFB78_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC1269301.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18020..>18197
                     /locus_tag="ACFB78_001324"
     CDS             18020..>18197
                     /locus_tag="ACFB78_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1269302.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEE"
BASE COUNT         3890 a         4981 c         5250 g         4076 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgcgccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgcg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gcttccgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat ggaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ttgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttaccc gcttcagcca cgacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact cgggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tctattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcgaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaacgaa accgaacgaa ccggattccc gcttttacgg gaatgacggc
     5881 atatcggttt ccgtgaggac aaacctagat tcccgcctgc gcgggaatga cgggatttta
     5941 gattgcgggt atttatcggg aatggcgatt taaaaattac ctaaaactaa acttaaaagc
     6001 aactgaaact taaaaaacta aaatttcgcc tgcgtgggaa ggatgggatt tcagatttct
     6061 gtttttgtgg gaatttgccg tatcgggctt cagacggcat tgcctgccgt tttgcccccg
     6121 ggtgcggctg ccttgatgta gttgagcgag acaaactgct tctcggcatc caattcggtg
     6181 attttaaaca atgcctgcga tttgggcagc gcgtcaaacg gaataccggt cgcgcgcgtt
     6241 gtcagtggca gaccttcgat gcggacgagg tcttctttca gaatcgtcgc ggtcagttcg
     6301 ctgatgcctt gttgttgcag gtacacgagg ctccagtagg cttccatctg ccgttggaag
     6361 tcggcgtagg cggcgtaggc ggtgtcgaaa tcgcgcaggg cggcgaagag cgcggcatcg
     6421 cttttttgat acagcggctc ggcagtgtcg tcgatcaggc tgatcagctg cttttggttg
     6481 atgtagtcgg cggcgcggcg cagcggcgag gtaaaccagc cgtaatgctg cacgcccatg
     6541 ccgatgtgcg gctcggattg ggtgctcatg cgcactttgc ctgccggttg gacgcggaaa
     6601 aggccgggca gcccgttttc gtctagcatt tgcgcccaag tgctgttggc aagaatcatc
     6661 atctcgctga ccagcgtatc gatgggcgaa ccgcgttcgc ggcggacgac ggagacgttg
     6721 ccttcttcat ccaattcgat gctgtaatcg tattgcggcg cgcggtcggg ttcgtatttg
     6781 ccgcgcgctt tttgcagggc ggtggcgaat tgatagaacc aaatcaggtc ttgatggtgg
     6841 gcgaacatca tttcgcctgc ttggtccaag ccggtttctg cgttgaaatg cggctcgatg
     6901 gcttggatac gcaggtttgc ggcgatgttg acggcttcga ttttgcaggt cggctcgccg
     6961 acgttgaact cgccgtccac atcgaaataa atgctgacgg cagggcggta tgcgcctgca
     7021 tcgaggctga acgcggtaat ccagttttcg ggcagcatcg tgattttgcc gccggggaaa
     7081 taaaccgtac tcaaccgctg cataatgatt tgttccattc cgccgccttg tcggacggca
     7141 agcgacggcg cggcgatgtg gatgccgacg cgcttcgtgc cgttgcccaa atcggtcagg
     7201 ctcaaggcat cgtccacttc ggtggtcgat tcgtcgtcaa tggaaaaggc ggtaacgtcg
     7261 gccttgggca ggtcgggcat ttcgggaagg gagaggtcgg ggaaacctgt tcctttgggg
     7321 aagtatttga tttcaaagcc gtcttgcagg tattgcggaa tggacgtaat gccgcccgtt
     7381 tttttcgcca gttcgtaggc ggaggttttc agtgcgtcgg cggctttggt aaaggctttg
     7441 taggtcagcg actgtttgtc gggcgcgtgt aagatggttc tcaaatccgc cgcgatttca
     7501 gacggcatct cgccgcgttt caaggcttcc gcccaagcgt cgatttgcgc gtcttgctgt
     7561 tttttgcgtt cgatggcggc aagcgcttgt ttcaaagttt cttcgggcgc ggctttgaac
     7621 acgcctttgg cttttttgta gaaatacacc ggcgcggcgt acagcgcaat caaagtcgcc
     7681 gccagctcgg ttttggtcgg cgcatggccg taatattctt cggcgatggc ttcggcggta
     7741 aactcctctt cgccgcatac ttcccacaat aaatcggtgt cgatgtccgc cgcctgcgcc
     7801 tgagcgtttt ccaaaaacgc cgccatatcg ccgtcaaact cggcaaagac attgtttgcc
     7861 ttcactttgg tgcgtttgcc gtgttgggta tcgacttggt aggtagcgtc gtttttttgg
     7921 acgacgacgg caactttgaa ctggccggac tcttcgtaaa aaatattcat ttttcggatt
     7981 tttctgtgga aactcaagcg ggcgatttta gcagattacc gaaaatgccg tctgaaaaaa
     8041 ggttggggga aggcgggtgc ggctttgcgg tgcttgcgtt gcgtttttga cggggtacaa
     8101 tcgccgtttt tgaacggagg acggaatgga gaatgtaaac cgcgtgccgg agcaggcacg
     8161 ttatgatgcc gaacgcaggc aggcagacga agcattggcg gacgtgtttc cggcagtcag
     8221 tattttcggc agcgcgcgca cgccgcagaa tcatacggat tatgcgtttg cctgccgtct
     8281 ggcgcggcgg ctgtcggatt cgggtattgc cgtcatttcg ggcggcgggc cggggattat
     8341 ggaggcggca aacaagggcg cgtttgccgg gaagtcggtt tcggtagggc tgaacattgc
     8401 cctgccgcac gagcagaaac cgaatccgta tcaggacatc gccttgcggt tttcccgttt
     8461 tgccgaacgc aaggcagtgt ttttccgcta ttcccaagca tatgtcgtga tgccgggcgg
     8521 cttcgggacg ctggacgaat tgtttgaaat cctgaccttg gtgcagacgg gcaaagtgcc
     8581 gccgcgtccg gtcgttttgg ttggaaaggc gttttggtcg ggcttggcgg agtggataaa
     8641 cgcgcagctt ttggcgcgcg gtatgatttc cgaaggggcc gcttctttgt tctccatatc
     8701 ggacgacgaa gacgaaatca ttgcgtatct gtcggaacac gggcttcaga cggcatagcg
     8761 tcctgagagt gatgtataat tgcaaacaat ttgtcaattt ttgatgtctt ttccgaacag
     8821 gatgccgata tgatcaaccc catcgcctcg ctttcccccc tagacggccg ttatgcccaa
     8881 tccgttgaag cattgcgccc gattttttca gagtacggac tgatgaaggc gcgcgtcaaa
     8941 gtcgaattaa actggctcaa agccctggcc gccgcgccgg agattgccga agtgccgccc
     9001 ttcagtgccg aaacgcttgc cgaaatcgac aaagtcatcg aaaacttttc attagaagac
     9061 gcggccgccg tcaaagccat cgaagccacg accaatcatg atgtcaaagc catcgaatat
     9121 tggctgaaaa aacgttttgc cgaagtgccg gaagtcgccg ccgtgagcga gttcatccac
     9181 ttcgcctgca ccagcgaaga catcaacaac ctgtcccacg ccctgatgct gcaagaggcg
     9241 cgcgaaaccg tcatcctgcc caaactggca gaaatcatcg gaaagctcac cgatatggcg
     9301 cacgagcttg ccgccgtccc gatgatgagc cgcacccacg gccagcccgc cacgccgacc
     9361 actttgggca aagaaaccgc caatgtcgtg taccgcctgc aacgccagtt caaaatcctt
     9421 caggcgcaag aatttctcgg caaaatcaac ggcgcggtcg gcaactacaa cgcccacatg
     9481 gccgcctatc ccgatgtgga ttgggaaaca cactgccgca acttcgtcga aatcagcctc
     9541 ggcctgacct tcaaccccta caccatccaa atcgagccgc acgactatat ggcggagttt
     9601 ttccaaaccc tcagccgcat caacacgatt ctcatcgact tcagccgcga cgtttggggt
     9661 tatatttcat tgggttattt caaacaaaaa gtcaaagcgg gcgaagtcgg ctcttccacc
     9721 atgccgcaca aagtcaaccc catcgacttt gaaaactccg agggcaacct cggcatggca
     9781 aacgccgtat tgggcttttt ggcggaaaaa ctgcccgtct cacgctggca gcgcgatttg
     9841 accgacagca ccgtattgcg caatatgggc gtgggcgtgg gctatgccgt gttgggtttc
     9901 gccgcccacc tgcgcggtct gaacaagctc gaacccaacc ctgccgcgct tgccgccgat
     9961 ttggatgtca cttgggagct gctcgccgag ccgattcaaa ccgtaatgcg ccgttacggt
    10021 gtcgccaatc cttacgagaa gctgaaagac ctgacgcacg gtaaagacgg cattacgccc
    10081 gaagtgttga aactctttat cgaatcgttg gaaattccgg cagaagccaa agccaaattg
    10141 cttgagctga cccccgcgct gtatgtgggc aaggctgaag cgttggcgaa acggatttga
    10201 gtgcttattg aaaccgatgc cgtccgaacg cgcgttcaga cggcattttt aagataacgg
    10261 gacatacggg gcgatattta tgcaagctgt ccgatacagg cctgaaattg acggattgcg
    10321 ggccgtcgcc gtgctatccg tcattatttt ccacctgaat aaccgctggc tgcccggagg
    10381 attcctgggg gtggacattt tctttgtcat ctcgggattc ctcattacca acatcattct
    10441 ttctgaaata caggacggtt ctttttcttt ccgggatttt tatacccgca ggattaagcg
    10501 gatttatcct gcctttattg cggccgtgtc cctggcttcg gtgattgcct ctcaaatctt
    10561 cctttacgaa gatttcaacc aaatgaggaa aaccatagag ctttctacgg tttttttgtc
    10621 caatatttat ttggggttcc gattggggta tttcgatttg agtgccgacg agaaccccgt
    10681 actgcatatc tggtctttgg cggtagagga acagtattac ctcctgtatc ctcttttgct
    10741 gatattctgc tgcaaaaaga caaaatcgct acgggcgttg cgccacatca gcatcatcct
    10801 gttcctgatt ttgactgcca catcgttttt gccggccgcg ttttataccg acatcctcaa
    10861 ccaacccaat acttattacc tttcgacact gaggtttccc gagctgttgg tgggttcgct
    10921 gttggcggtt tacgggcaaa cgcaaaacgg cagacggcaa acagaaaatg gaaaacggca
    10981 gttgctttca ttactctgtt tcggcgcatt gcttgtctgc ctgttcgtga tcgacaaaca
    11041 cgatccgttt atcccgggaa taaccctgct ccttccctgc ctgctgacgg cgctgcttat
    11101 ccggagtatg caatacggga cacttccgac ccgcatcctg tcggcaagcc ccatcgtatt
    11161 tgtcggcaaa atctcttatt ccctatacct gtaccattgg atttttattt ccttcgccca
    11221 ttacattaca ggcgacaaac agctcggatt gcctgccgta tcggcggttg ccgcgttgac
    11281 ggccggattt tccctgttga gctattattt gattgaacag ccgcttagaa aacggaagat
    11341 gaccttcaaa aaggcatttt tctgccttta tctcgccccg tccctgatgc ttgtcggtta
    11401 caacctgtat tcaagaggga tattgaaaca ggaacacctc cgcccgctgc ccggcacgcc
    11461 cgttgctgcg gaaaataatt ttccggaaac cgtcttgacc ctcggcgact cgcacgccgg
    11521 acacctgcgg ggttttctgg attatgtcgg cggcagggaa gggtggaaag ctaaaatcct
    11581 gtccctcgat ccggagtgtt tggtttgggt ggatgagaag ctggcagaca acccgttgtg
    11641 ccgaaaatac cgggatgaag ttgaaaaagc cgaagccgtt ttcattgccc aattcttatg
    11701 atttgaggat gggcggccag cccgtgccga gatttgaagc gcaatccttc ctgatacccg
    11761 gattccaagc ccgattcagg gaaaccgtca aaaggatagc cgccgtcaaa cccgtctatg
    11821 tttttgcaaa caacacatca atcagccgtt cgcccctgag ggaggaaaaa ttgaaaagat
    11881 ttgctataaa ccaatatctg cgccccattc aggctatggg cgacatcggc aagagcaatc
    11941 aggcggtctt tgatttggtt aaagatattc ccaatgtgca ttgggtggac gcacaaaaat
    12001 acctgcctga aaacacggtc gaaatacacg gacgctatct ttacggcgac caagaccacc
    12061 tgacctattt cggttcttat tatatggggc gggaattcca caaacacgaa agcctgctca
    12121 agcattcccg aggcggcgca ttgcagtagc ctgccttctt gccggatatt gcctttggca
    12181 gcctatgccg ctgtttgccc tttgaggcgg cttttatttt gccgttttga ggccggggtc
    12241 gggaataacc gttttttgat gattttccct ccccggctgt gtcatcaaat ccctagttgc
    12301 ctttccaaac tctccaccag attgtcatcc agttccaaag cctgcgacag gcgggcgagg
    12361 aagacggttt ctttccgcga caaatcggca cagaccaacc ttgccgccag ataggtctcc
    12421 gccgccaacg cctcatcgtt gccgacggcg gcggcgatgt ccccgatgct tgcgggcagg
    12481 cggtattcgg cggcgagcca tgcggcagtt tcggggtctg tgccgctttc ctgttcgata
    12541 gtccggcgtt cggcttcgtc tatcatgccg tctgaagcgg cggcggctat catggtacgc
    12601 aatacggtgc ggctgtatgt ttcttcggtt tttccggcag gttggaaatc gctttgtgtt
    12661 acggttgccc gccctttgtt ttgctgccac atctgatagc cccggtaggc gaggtagccc
    12721 aaagcggcgg tcgaaccgat tttggtgatg gttttgcggt ttttaccgtt cagcagcatg
    12781 gaggcgacac cggcaaccag cgcgcctccg ccgaatgaat tgagcgggct gccggagaat
    12841 gtgctgcctt ttttttgaac cgtgcttaag acttggttga gcaaccgggt gaagttcatg
    12901 atttttcctt tctgttgcgg gaagggcggt atgtttaccc tatcctttta aacggtggca
    12961 ggccggccaa taattgttgg ccgtaccgct gcgttttgat gcggttgtcg aggatggtta
    13021 cgcggccgta gtcttgttcg gtgcggatga ggcggccgac ggcctggatg agtttgatgc
    13081 cggcttcggg gacggtgatt tcgatgaagg ggttgccgcc gcgttgttct atccaacggt
    13141 tttgggtttt ttcgatgggg ttgtcgggca tggcgaaggg gagtttggcg atgatgactt
    13201 gcacgcaggc ggtgccgggc aggtcgagcc cttcggcgaa gctgtcgagt ccgaagatga
    13261 tgctggcttt gccttcttct atggcttggt ggtgtttttg caggaggacg gctttgggta
    13321 attcgccttg tacgagcaag agcggcaggt agtctccggg caggtgcagg gcgacatcct
    13381 gcatttgttt gcgcgaggaa aacaggacga gtgtgccgat ggcttcggtg ggcgaaatca
    13441 gcttgggcag ccattcgatg acggcggcgg tgtgggcttc ggggtctttg gggctggcgt
    13501 atatggaggg gatgtagagt tcgccctgtt tttcaaagtc aaaggggctt ttgagggcga
    13561 gggtggtggt ttcgggcagc cacagcaggc cggtttggcg caaaatcaag ttgaagctgc
    13621 cgagcgattg cagggtggcc gaagtcagca ccgcgcctgc cgcgcgccgc cacaggctgt
    13681 tggcaaggtg ggacgcgctg ctgatggggc tggcattgaa aatatagtcg tttttgtcgt
    13741 cggcacggcg ggctatccat ttcgccaacg gctcctcgcc ctcaatggaa acagtggaga
    13801 gcaaatccca aaccgcgctg atttgttcga tacgggcgat aaaaaggccg aactcgccgg
    13861 tcaggcggtc gatgagcgca ccgtcctgtt ctttttcgcg gcgtgcggcg gaaagcgcat
    13921 cgttcagccc gacaacgtgt ttgagcaggc tgcgtgcagc aatggccgta ttggaaacgg
    13981 tggtttcgag gccttcgggg attttgccgt cttcccacag ccaagtcggt tcgctgttgg
    14041 ttcgtctgtc gttttcagac agccccatac ttaaagacgg ctcttccgcc aaatggaatt
    14101 gccattcgtg caggctgtcg agcaaggatg cggcggcttc gtcggcaagg ttggcaagtt
    14161 cggctttatc ggtaagcgcg gcaattttgc cggtaagctg cggcagtttt tccagcgtcc
    14221 aaacggcaat attccatgaa tgttcggcgg caaaacggct gagggctttt ttgggcaggt
    14281 ggtgcgcttc gtctatgcaa tagaaactgt tttcgggcgc aggcagaatc acgccgccgc
    14341 ccatgctgat gtcggcaagc agaagatcgt ggttggcaac aacgacatcg acggtttcca
    14401 acatatcgcg tgccaggtaa aaagggcatt ccggacggtt gggacaggcg gctttcaggc
    14461 agccgtggcg gtcgttggtc actttgagcc aaatcgcgtc atcgattttt tccggccaag
    14521 cgtcgcggtc gccgttgaac cgtcgggcgg aaaattcgtc ggcgatgtcg cgcagcagct
    14581 tcaattcttc cggcttgggt ttgctgtccc acaagacggc gggggcttca aagccgagca
    14641 ggttttgctg ggcgttgctt tgcgtcagtt gatagagttt gtaggggcag agatagcggc
    14701 cgcgcccttt ggcaagtgcg aaggtcagtt ccaaaccgct tttttcgacc agaaacggca
    14761 ggtcgcggtc taccaactgc tcctgcaagg caaccgtcgc gctgctcaca atcagccgct
    14821 tgccgcgtgt ttgcgccatg atgccgcccg caagcaggta ggcaagcgat ttgcccacgc
    14881 cggtcggccc ttcgattacg gcaatgcttt cgccctcgcg cttgggcggc tcgccgcctt
    14941 cttcgcgcgt caacgtccgc gaaaaagcgt tggcaaccgc cgcaatcatt tcccgctgcg
    15001 aagcacgcgg acggaaatcg ggcaggtttt tgccgatatt ttggtaatgg tcgcggatgg
    15061 cgtttttttc taaatcggtg agcatagtat cggtgggcgg atgatttggg gtcattttaa
    15121 cattgcgcgg atgcggtacg gtatcgttgt cggacggtgg ttcgggcata tctgattatt
    15181 gaaagtaaaa aaacggaaga tattgggtca tccgtacaaa atggcgtctg aagcgggaaa
    15241 cgggcttcag acggcattgc cgcgcttata cgccggcgac ggggcgggcg ggatagccgt
    15301 gggtatcgac cgaacaggca aaccgccaag gcgtgtggac ggtgtcggcg gacaggtggg
    15361 cgagttcggg aatgtgccgt ttgataaaag taccgtccgg gtcggtacgg tatgaggcgg
    15421 cggcaatgtc ggggcaggtg tgccgtgagg cggcaagccg ccagttgcct tggttgattg
    15481 ccgcatcgaa atcggtcagc tgccgggcaa accatatctc gccttcgcgg cgggggaggt
    15541 ttaaaacgtg gcagaaaaaa tccgcgctca agcgtctcag ggcggggtgg aggctgccgg
    15601 ttttgtgcaa acagcgcatc gcggcatcga taatcggaat gccggtccgg ccctgctgcc
    15661 aaagcgtcag gcgcagggtg tgttccggat tgccgtctga agggtcgtca tccgcgtgct
    15721 gcaaggcaag ttgaaggaaa aaatcgcggc ggatgatgtt gtccgcccac gcgttcagac
    15781 ggcgttcgag gctttcccgc gcgagcaggc gcggcgagat gcagccgaca ctcaaatacg
    15841 cgcccatcag cgaagtgttt ttgcgcgaag ggaaatcctt taaaacggag taggaagccg
    15901 cctgttcgag aaaccgccgc cactgccgcc aagccgccgt ttcgccgccg ttttgcggca
    15961 ggaagatgcc gtctgaaagc gcggcaggcg gcggggcggg caggttttcg gggaagggtt
    16021 ggcggtatgc cgcgaatagg tccgaaccgg cggggggctg cttggaaaag cggtcgagcc
    16081 atacttcgcg gtagcggttg aaatcggtgt gtgccgtacc gttgtctggt gtcaggtcgg
    16141 ttttgccgaa aacggaacgg tcgttgacga aggttaacgc gatgccgtgt ttgtccaatt
    16201 cgtgccaaag ggcgttgtcg gcgagtttgt cggcaaaagt atgggattcg tcggcgatga
    16261 cggcgcggat attgaggcgg acggcgagcc ggacaagccc ggcaggcgat gccgccgtgt
    16321 agagcgggat gccgcgcctt gccagccctt gggcgagttc ggcggcggat tggcggtaga
    16381 acgcggcgcg gcgcgggttg tctgtttcgg catcgtcaat gcaaatgccg ataacgggcg
    16441 aaccttcggc aacggcggcg cataaggcgg cgttgtcgcg gatgcggagg ttttggcgga
    16501 accagacgag cgtgtgcgcg gcgcgcgtgt ccgcataaag ggggagggcg gtttcagacg
    16561 gcatttcggc agcctttcct gctggcgatt ttttcgttca gaaaatcgat gaaactgcgg
    16621 actttcgcac tcaaaaatgc cctgtccgca taaacggcgt tcagccggtc ggtcgggacg
    16681 gcgtatccgg gcagcagcct caccagcggg ccgcagcgca aatcgtgttc cgccgcccaa
    16741 agcggctgat aaccgatgca cgcgcccgcc ttaatcattt cgcgcatcat cagcgtgttg
    16801 tcggtacgga tgacgggggt cagttcaagc cggtatttct tgccgtccga tttgcgggtg
    16861 aggtcgagtt tctgctggtt ggtgtaggtc ggcaggacgg caggcagccc cgccacttct
    16921 tccggtgttt ccggcacgcc gttgcgcctc aggaagtcgg gcgaggcaag cagggcaaat
    16981 tcgatttccg ccagcgggcg cgcaatcagc gaaggggaaa gggtttggga aacgcgcaac
    17041 gccaaatcca cgccttcggc aatcaaatcg acgtggcggt tgtccaaaat cagttccaac
    17101 gccacttcgg gataacgttc gcggtattcc gccagccagt tgcatatctg gctgccggca
    17161 aaccacagcg gcatcgttac gcgcagcagc ccctgcggtt tttccgtccc cccggcggct
    17221 ttttgcgcgg catcgtcgag cgtgtcgagc gcgtaactgc attgccggta gtattcttcc
    17281 ccggcttcgg tcaggctgag gttgcggctg ttgtggtgca ggagtttggc ttggacggtg
    17341 ttttccaagt gactgacgtg tttgcttgcc attgcggtgg agatgccgag cgcgtcggcg
    17401 gcgcgggtga agccgccgct ttggacgact tggcggaaaa ctttgaggct gaacagggtg
    17461 tccatatttt cttgtgtgga aaagttgtat caataaaagc agtatatatt tgaaaaaggg
    17521 aaacatctat actctgccgc ctgaaatgaa gacaaatatc aaaggagctt ttatgtccga
    17581 ttgctgcaac cgtatccaac cggttttgct ttctgttttg cgtatcgtaa ccgcctacct
    17641 gtttttgttg cacggtacgt cgaaaatctt cgccttcccc attgaaatgg gcagcggttc
    17701 gcccggcggg ctgttgctgc ttgccggtat tttagaaatt gtcggcggca ttttgctggt
    17761 gttgggcctg tttgcgcgcc ctgccgcgtt tgttttgtcc ggccagatgg cggttgccta
    17821 ttttatggcg cacgcttccg gaaatgcttt gttcccgatt gccaacggcg gcgagtccgc
    17881 agtgctgttc tgcttcgtat ttctctatat cgcggcagcg ggcggcggag catggtcgct
    17941 ggacaggctg tttttcaagc gtaaagcctg aaccggactg cctaaagtgt attttgttga
    18001 atgtttttga ggaaaagaaa tgagccgtca atccctgcaa caggctgccg aaagccgccg
    18061 ttccgtttat tcgttaaata aaaacctgcc cgttggcaaa gatgaaatcg tccaaatcgt
    18121 cgaacacgcc gttttgcaca cacctttttc gttcaattcc caatctgccc gcgtggtcgt
    18181 gctgtttggc gaagagc
//
LOCUS       DAXDZG010000046        18060 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXDZG010000046 DAXDZG010000000
VERSION     DAXDZG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18060
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.046"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..1257
                     /locus_tag="ACFB78_001325"
     CDS             <1..1257
                     /locus_tag="ACFB78_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC1269303.1"
                     /translation="PKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASA
                     EGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVAVGSEAKVNAERGIAIGKEAKA
                     GGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGD
                     FSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHS
                     VSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVND
                     FDAVNVRQSKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFG
                     TYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKV
                     LMEQLMQDNRQLKPRLEKLEARLNAM"
     gene            complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="ACFB78_001326"
     CDS             complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="ACFB78_001326"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC1269304.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2041..2724)
                     /locus_tag="ACFB78_001327"
     CDS             complement(2041..2724)
                     /locus_tag="ACFB78_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC1269305.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAPLAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2881..3147)
                     /locus_tag="ACFB78_001328"
     CDS             complement(2881..3147)
                     /locus_tag="ACFB78_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC1269306.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="ACFB78_001329"
     CDS             complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="ACFB78_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC1269307.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYAFKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="ACFB78_001330"
     CDS             complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="ACFB78_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC1269308.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="ACFB78_001331"
     CDS             complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="ACFB78_001331"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC1269309.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIRKHGLYEK"
     gene            complement(4791..5390)
                     /locus_tag="ACFB78_001332"
     CDS             complement(4791..5390)
                     /locus_tag="ACFB78_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC1269310.1"
                     /translation="MSELSEILSYNQKFVESGGYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5419..6960)
                     /locus_tag="ACFB78_001333"
     CDS             complement(5419..6960)
                     /locus_tag="ACFB78_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1269311.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7351..8736
                     /locus_tag="ACFB78_001334"
     CDS             7351..8736
                     /locus_tag="ACFB78_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC1269312.1"
                     /translation="MDGRTQTLSAQTLLGISAAAIILILILIVKFRIRALLTLVIASL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFAPGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASVGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRAIHVPVP
                     ELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPVCPVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTANQTLIALIG
                     FALSALLFAIV"
     gene            8756..9274
                     /locus_tag="ACFB78_001335"
     CDS             8756..9274
                     /locus_tag="ACFB78_001335"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC1269313.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9460..10377)
                     /gene="thrB"
                     /locus_tag="ACFB78_001336"
     CDS             complement(9460..10377)
                     /gene="thrB"
                     /locus_tag="ACFB78_001336"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC1269314.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10414..11142)
                     /gene="ubiG"
                     /locus_tag="ACFB78_001337"
     CDS             complement(10414..11142)
                     /gene="ubiG"
                     /locus_tag="ACFB78_001337"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC1269315.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11289..12530
                     /locus_tag="ACFB78_001338"
     CDS             11289..12530
                     /locus_tag="ACFB78_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC1269316.1"
                     /translation="MSAKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLIVLL
                     YTWFSMLSGGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12682..13737
                     /locus_tag="ACFB78_001339"
     CDS             12682..13737
                     /locus_tag="ACFB78_001339"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1269317.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTEL
                     PVKLAVAGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDMLASV"
     gene            complement(13804..15456)
                     /locus_tag="ACFB78_001340"
     CDS             complement(13804..15456)
                     /locus_tag="ACFB78_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC1269318.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIDYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15599..16162
                     /gene="gmhB"
                     /locus_tag="ACFB78_001341"
     CDS             15599..16162
                     /gene="gmhB"
                     /locus_tag="ACFB78_001341"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC1269319.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16192..16935
                     /locus_tag="ACFB78_001342"
     CDS             16192..16935
                     /locus_tag="ACFB78_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC1269320.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16932..17621
                     /locus_tag="ACFB78_001343"
     CDS             16932..17621
                     /locus_tag="ACFB78_001343"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC1269321.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILAPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARATQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQRI
                     KIHGRELFALG"
     gene            complement(17685..17876)
                     /locus_tag="ACFB78_001344"
     CDS             complement(17685..17876)
                     /locus_tag="ACFB78_001344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269322.1"
                     /translation="MPSENLSDGIFAGKSVEGFAPAQAFHTARRIGRVSEGRCEMKRC
                     GRRGCRHPAPNRRQSVIAV"
BASE COUNT         4295 a         5461 c         4594 g         3710 t
ORIGIN      
        1 cccaagaaat atgcagtagc attaggatct agttctactg ccagtggaga gtattcctat
       61 gcgtcgggct ataattctgt tgccagtgga aataaatcct atgctgcagg ctatgcttct
      121 gttgccagtg cagaaggcag tgttgtaatt ggcgattcaa ggcaagtaaa accggaagca
      181 gatcaagggg ttgcagtagg ttctaaagca acggtaaaga acaaagctaa acaaagggtt
      241 gcggtaggtt ctgaggcaaa ggtaaatgca gaacgtggta ttgcgattgg taaagaagcc
      301 aaagcagggg gcaaaacaac gaacacactg ttagatggac cggcatatta tgcggacgct
      361 attgccgtcg ggtatcaagc agaggctggc aaaggtggcg ccatcgcttt gggaaaacaa
      421 gcaaaagcaa cgaagcaaaa tggaatggca cttggcgtag aatcagaagc tgccggtgat
      481 tttagtaccg ccgtgggtaa tgaaagcaag gcaaaggggc aaggcggcgt aggtctcggt
      541 aaccaatcta aggcagaggc agattttgcg gtggctgtgg ggaataaggc tgaggcgact
      601 aaggaaaact cacttgtcat tggccgatat gcaagggcaa acggaaatca ttctgtgagt
      661 ttaggatctc gtagtgaaat aaaagatggt gtgagcaatt ctgtggcacc gggttatggt
      721 tcagtagcta gtgagaataa tgtcgtctct gttgcctata aagaaacacc acaatctaca
      781 gaactttcct atagaaaaat tgtaggtgtt gatgatggtg tcaatgattt tgatgccgtc
      841 aatgtacgcc agtcgaaagc gatgcaagga caaaatatgg ctgagctatt ttcagtacgc
      901 agcgaagtgc gtggtgtggc agcgagtagt gcagccctat cggctctgac tccattatcc
      961 tatgatgcaa acaatcctac gcagtttatg gtcggtttcg gcacatacaa aggacgtcaa
     1021 gctatggcat taggattgtc ccatttcgtc aatgaccgct ttatggtaaa agtaggcgga
     1081 accttaggcg gcaataagac gggacatatg atgaatgtag gcttgacttg gaaatttgga
     1141 acgagccaag gtgttccggc tacaagtgat aaggtgctta tggaacagtt gatgcaagat
     1201 aatcgtcaat taaagccacg attggagaaa ttagaagcac gtctgaatgc tatgtagcct
     1261 tgtggcgata acggtttatt gagtatatcg ccaatgataa agtgcataat tttcaaaccg
     1321 ttgataatat acaataaatt gtaaccaata cctcaaacgg gtttgaaccc gaatatgcct
     1381 gacaggaact ttctgtcagg cattgttgtt gtgatgttct gcaatcattt tttggtacac
     1441 ggccggcggc acatatcttt tgacggtttc catccagccc tcgggcccga ccagtccttt
     1501 gatcatagtg gacgacactt cggcgatttc gcgcggcggc atgaggaata cggtagatat
     1561 ttcgggtgcg aggtcgctgt tgatatggcg catggagcgt tcgtattcat aatcggaggt
     1621 ggaacggatg ccgcgcacga tgaatcccgc atctacctca cgggcgtaat gcaccaaaaa
     1681 ccggttttga aacacttcaa ttctgacgtt tgggaagttg tcggtaatgg cgcacaacat
     1741 atcctgcctt tcagcggcgg tataggtgtt gcgtttgtcg ggattgatgc cgatggcgac
     1801 gatgagttcg tcaaacatag attgcgcctg ccgtatcatc cacagatgcc ccaatgtggg
     1861 cggatcgaaa ctgccggcat aaacggcgcg gcgcggtgta ttcggtaaca tttgattcct
     1921 cccggcttca tagccggctg tgtttttatg tgtttgcatc cgtattgtat gcccaaaaca
     1981 aaatgccgtc tgaaaataag tgctttcaga cggcatggtt tgcatggtgt ttcgcctaca
     2041 tcagtcgtcg ccgtttaagg acagcaggat gttgagcagg ctgctgaaga tgttgtaaag
     2101 cgagataaac agtgtcagtg ccgcgctgat gtaactgtct tcgccgccgt cgatgacggt
     2161 gcgtacctgc cacataatca ttaaggaact gaacaagaca aaaccggcgg aaatggtcag
     2221 ggcgagtgcg ggaataccca aaaacagatt ggcaaccacg gcgaccatca gaatgaccgc
     2281 acctacggtc aggaagcgtc cgagcgcgtt catatcgagc cgggttcggc gtgccaatgc
     2341 ggacatcgtt aaaaagacgg cggcggtcat cgcggcggca atgccgacga ttttcgcacc
     2401 gtcggcaata tggagtgcgt attgcagcac ggggccgatc aatacgccca taccgaatgt
     2461 gaataccatc agcagggtaa cgccggtatt gctgtaacgg tttttttcga tgaagtggat
     2521 cataccgtag aaaaacgcca atacgacggc aaatcctatc cagcgcgaac cgaaggcggc
     2581 gtaaaaattg aaaccggcat tggcggcaag tggcgcgcct gcggctgccg gaatgaatga
     2641 aaatccgagc aggcggtagg ttttctgcag gacggtgttt ttagaaaccg tatgcgcggt
     2701 gtagtcgtaa acgtcgtgtt gcatatcatc tgctcctgaa agcgcggttg gaaataacgg
     2761 gggattttaa cattgcccaa tgtcaaaatt tgtccggttg cgtgaagata aagttgtccg
     2821 gcgtatttta aaggccgtct gaagcagttt cggacagcct gtgttcaaaa cggaaaaccg
     2881 ttattgcgga acgtatccct gcacggcatc cgcgccgtcg ccgaagaaat actgctccat
     2941 ctgctgagcc aggtattcgc gcgcgcgcgg atcggcaagg cttaagcggt tttcgttaat
     3001 cagcatggtt tggtggcgcg tccacgccgc ccatgcttct tgcgatacgt tttcaaaaat
     3061 gcgtttgccc aattcgttgg gcagcggcgg aaatttcatg ccttcggctt ctttgttgag
     3121 cttgacgcag aataccattc gtgccatagc ggattccttt gctgtgttca gaaataacgg
     3181 ggcgatttta accgattagg gggtacagac aaaagccttt ttattcccga tgataaggat
     3241 ggttgtgcag gatggaaacg gcccggtaga gctgctcggt cagaaggacg cgcaccatgc
     3301 cgtgcggcag ggtgaggctg gacaggcgca tcatcatgcg ggcttgttgt ttgaggcggt
     3361 cggtcatgcc gtccgcgccg ccgatgacga agcagacgtg ttcgccgttt tgccgccagc
     3421 ttttgaggtg ttccgccagc tcgacggagg tcggtgcttt gccgcgttcg tcaagaacga
     3481 cgaggaacgc gccttgcgga atggcttcaa ggatgcgttt ttcttccgcc gccatacctt
     3541 ggacggcatt cacgcccgcg ccgcgttttt cgggtttgat ttctttgaat gcgtaggcgg
     3601 cgtcgcgtcc gaagcgtttg gcgtattcgg cgacggcctc atcaacccaa cgcggcattt
     3661 tggtgccgac tgccaagacg gtaatgttca atgctttccc ccttacagga aatgccgtat
     3721 gaatattcag acggtatcgg gaatcagtct gcagcgtgcc acggcttctg cattccggcg
     3781 tggaaactcg gtttctcgcc gccccagatg gtgtcaatgt cgtagaagtc gcgcacggca
     3841 gggagcatga cgtggacgac gaggtctcct gcatcaacca acgtccattc gccgctgtct
     3901 ccttcggtac tgaggatttc aaaaccggct tctttcaaat cgacggcaac gttgttggcc
     3961 agtgctttga cttggcgcgt actgtcgccg ctggcgataa tcattctggc aaacagcgaa
     4021 gttttgtctt gcgtttcgag aacggaaatg tctttggctt tgatgtcttc gagggcattg
     4081 acggcgaccc cgaccatttt ttgcaggtct tgcagttctt gttcgttcat tgttttccta
     4141 acgggatgtt ttcagacggc attatagcct ctccttactg atttgacttt atttttcata
     4201 caaaccgtgt tttcggatat aacgggcggc ggcaggcggg atgccgtctg aaacaccggc
     4261 ggcactcagg ttgcggcgga tttccgttga cgacacatta tgcatcgggg ctgacaagat
     4321 gcggacgctg ccgtcctgaa gggcattgcc cagccacgcg tgcagttcgc gcggggtttg
     4381 gtgcaggctg tcgccctgcc gcatggcgac ggcgatattg gtttcgcgca cgagcaactg
     4441 ccattttttc caagtatgca gcttcagcag gctgtcgctg cccatcagcc accaaagttg
     4501 cgcggacggg aactgccggc ggaagatttg gacggtatca aaagtatagg ttgcactttc
     4561 tcggacgatg tcgcagtcgc tgacggcaaa acgcgcgtct tctgccgtcg ccagttcgac
     4621 catggcaagg cggtcggcgg cggaagcgga ggcggcgtct ttgtgatacg ggccgcctgc
     4681 cggcaggaaa acaaccgcgt ccaacccgat ttcgtcggca aaggcacggg cgatatgaaa
     4741 atgcccgttg tgtatcgggt cgaaagtgcc gccgaacagt ccgattttct tcatgatgtt
     4801 tccattcctt ccgataagtc catgccgtct gaaacactgc cgtccacaac cagcgtcagt
     4861 ttgccggtaa cgggatggat gaccagtccg tgaacggcga tatggcgcgg catcagcgga
     4921 tggttgcgga taaggtccac cgtgtggcgc acgctgtctt cgacgttgtc gaaaccggtc
     4981 agccagccgt cgaggtcgac acctgcataa cgcagggttt cgatacggtc ttcgggaatc
     5041 cggctttccc ggacgcgccc gaggaattct tcggcattca gcccctgcat accgcaatcg
     5101 tgatgggcga tgaccataat ctctctgacc ttcaattcaa acacggcaac caaaaggctc
     5161 cgcatcaccg aaccccacgg gtgcgtaacc agcgcgccgg catttttaat cagcttggca
     5221 tcgccgtttt tcaaacccaa cgcgtcgggc agcagcccga taatccgcgc atccatacag
     5281 gacaaaaccg ccagcccgcg ttcggggtat ttgtcggtaa agtatttttc atatcctccc
     5341 gactcgacaa acttctgatt ataggaaagg atttcgctca actcgctcat tttgccgtcc
     5401 tctgaaaaag ggttcgcatt ataacgtttc cgtccgtttt ccgccttcgc cgttgtccaa
     5461 cagcaggaaa atgcacaccg caaatgccga caacagcaat gtcaacacca ttgcccgcgc
     5521 ataattgtcc tcacccgcac gccccaaata ggcataaatc aaagtcgtca acgtctgcca
     5581 ttccggacgc gacaggaaca aggttgccgc aaattcgccc acacacgtcg ccgccgccaa
     5641 agtcagaccg cgccgcaacg ccggtttcaa gagggggaac gtgatacggc atgccgtctg
     5701 aaagccgttt gcgcccaaac ctgccgccgc cctgccgtaa tccggcggca gtgcatccca
     5761 ggccgataaa acatcttttg ccacaaacgg atacgccagc agcgcataca tcgccagcag
     5821 cagcggtaac gaagccgtcc accccggata aagcagcagc acgcccgccg aaacacaaac
     5881 cggcgacacc ataaacggta aaaacaccag tccgcgcatc cacgccagcc gccgcgccgc
     5941 cgccgcatac accacaccca aaaccgccgc cgcaaacacc gccgccgccg aaaagcgcaa
     6001 agtattccac actgcctgcc acgtttcact ttccattaac acacgccgcg attcgccggc
     6061 cgaccacgct ttcacaacaa ttgccgacaa aggaaacagg cagcacacgg acaacaccgc
     6121 caccgaaaat gccagcaata catattcccc caccgattgc ggcggcgacg gcatcacggg
     6181 ggaaaccgcc ttatccgaaa ccgcgcgcct gccgaaccac gcatacagca accctgccgc
     6241 cgccgttacc cccaacacca gccacaccag cgccgaagcc cccgccatat cgagttcgaa
     6301 cataaccaac tggtaaattt ccacttcgac cgtggcataa cggctgccgc ccaacagcaa
     6361 tgccagcccg aaccccgaaa aacaatacag gaagacaagg cacacgccgc cggcaagcca
     6421 cgggcgcaaa acgggcattt caatgtccca aaaccgccgc cacgcccccg cgcccaacgt
     6481 ccgtgccgtc tgaagccgtg ccgcaggcac ttgagcaaac ccctgatacg ccgccctgac
     6541 caacacgggc aggttgaaaa acacattgcc gtacaacaac agatacggcg tatcctgccg
     6601 gccgcgccac aacagcccgt ccgccccgaa cagagccagc acgcccacgc ccgccaccag
     6661 cgtcggcatc acaaacggca gcatcagcag gcgcagcacc aaagcccgcc ccgggaacgc
     6721 cagccgcgcc agcacccacg cgacaggcac gcccaaaggc agcaccagca cacaggttgc
     6781 cgccgcctga aacaccgtcc acgccaaacg tttgagcata taggcatccg acagcaccgc
     6841 gcgccacgcc aaaccgtcat acgccgccac cgcccacaaa ggcgcaacga ccattaccgc
     6901 caaaaaagcc gaaggcagca gggaaaaagc accccgtacc gcccaacgcc gtccatccat
     6961 cgccttcccc acttgaaaca ctgatgttgc gattgtaccc aaaagcccca cataccgtat
     7021 atttcaatcc gactacgtac cgtatccgcc ttcctcccgc cgtctgaaat atagcggatt
     7081 aacaaaaatc aggacaaggc ggcggaccgc aggcagtaca aatagtacgg aaccggttcg
     7141 cccggtgctt cagcacctta gggaaccgtt ccctttgagc taaggcgggg caacgccgta
     7201 ccggtttttg ttaatccact ataaatcgtt caaataaaca ggaatataac ttcagacaaa
     7261 caacttaccg cccgatttgt gctatcgttt tcgcacaact taaaaaaacc tgacaatttt
     7321 gtacttttat tacagagaaa ggctttacaa atggacggcc ggacacagac gctgtccgcg
     7381 caaaccctgt tgggcatttc ggcggcggca atcatcctca ttctgatttt aatcgtcaaa
     7441 ttccgcatcc gcgcgctgct gacactggtc atcgccagcc tgctgacggc tttggcaacc
     7501 ggtttgccca caggcagcat cgtcaacgac gtactggtca aaaacttcgg cggcacgctc
     7561 ggcggcgtgg cgcttctggt cggtctgggc gcaatgctcg gacgtttggt agaaacatcc
     7621 ggcggcgcac agtcgctggc ggacgcgctg atccggatgt tcggcgaaaa acgcgcaccg
     7681 ttcgcgccgg gcgttgcctc gctgattttc ggcttcccga ttttcttcga tgccggactg
     7741 atcgtcatgc tgcccatcgt attcgccacc gcacggcgca tgaaacagga cgtactgccc
     7801 ttcgcgcttg cctccgtcgg cgcattttcc gtcatgcacg tcttcctgcc gccccatccg
     7861 ggcccgattg ccgcttccga attttacggc gcgaacatcg gccaggtttt gattttgggt
     7921 ctgccgaccg ccttcatcac atggtatttc agcggctata tgctcggcaa agtgttgggg
     7981 cgcgccatcc atgttcccgt tcccgaactg ctcagcggcg gcacgcgaga cagcgacccg
     8041 ccgaaagaac ctgccaaagc aggaacggtc gtcgccgtca tgctgattcc catgctgctg
     8101 attttcctga ataccggcgt atcggccctc atcagcgaaa aactcgtgag cgcggacgaa
     8161 acctgggttc agacggcaaa aatgatcggt tcgacacctg tcgcccttct gatttccgta
     8221 ttggccgcac tgttggtctt gggacgcaaa cgcggcgaaa gcggcagcac gttggaaaaa
     8281 accgtggacg gcgcactcgc ccccgtctgt cccgtgattc tgattaccgg cgcgggcggt
     8341 atgttcggcg gcgttttgcg cgcttccggc atcggcaagg cactcgccga cagcatggcg
     8401 gatttgggca ttcccgtcct tttgggctgc ttccttgccg ccttggcact gcgtatcgcg
     8461 caaggttcgg caaccgtcgc cctgaccaca gccgccgcgc tgatggctcc tgccgttgcc
     8521 gccgccggct ttaccgactg gcagctcgcc tgtatcgtat tggcaacggc ggcgggttcg
     8581 gtcggttgca gccacttcaa cgactccggc ttctggctgg tcggccgcct cttggatatg
     8641 gacgtaccga ccacgctgaa aacctggacg gccaaccaaa ccctcatcgc actcatcggc
     8701 tttgccttgt ccgcactgct gtttgccatc gtctgacaga cggaaaggat agtaaatgac
     8761 tacgcatttt gtcgttacgg gcgtatgcgg ctgcggcaag accaccgccg cgccgtccct
     8821 gcagaaacac ctcggtcaat gtccctatgc cgaaggcgac gagttccaca cccaagccaa
     8881 ccgcgacaag atgggcgcgg gtattccgct gaccgatgaa gaccgctatc cgtggttggg
     8941 caatctgcgc gactggatga cgcaacaggc gcaaaacggc gcggaccaca ccatcgtaac
     9001 ctgttccgcc ctcaaacgcg gctaccgcga cattctgcgc ggagccgaag gcaaagccgc
     9061 cttcatccac ctcagtccgc cgcaagacat caacctcgaa cgcatgatgt cgcgcaaagg
     9121 gcactacatg aaagcaggga tgctcgattc gcaactggaa atcctcgagg aactgggcga
     9181 aggcgaatac ggggtcaaaa tcgccaaccc cggcacgccc gaagcggtcg aagccgatat
     9241 tctgaactgg gttgcctcgg aaaacctgct ttgaagcaat atgccgtctg aagcccggca
     9301 caggatgggt ttcagacggc ataaacatcg ggaacagaat gaattacatt gatttaaaat
     9361 ggaaaatacc cggctatcgt ctcattttcg ttttaatcag ccataaaaat gccgtccgaa
     9421 accctttcag acggcatttc tgtcaaacgc cggacgcact caatccaaac tcaacagcag
     9481 gttgcggaac gcgttcgggt ctttgataaa cgtcatctcg cccgcctgcg gaaaatgaaa
     9541 atccaacagg cgcgacaccc aaaaacggat gcagccggcg cgttgggcgg tcgggaaata
     9601 cgccttttcc ccggcactca aggggcgcac gccctcataa ccgccgataa acgccttttt
     9661 caacgcctca tccaacttat tgtccgccgt ccttgcccaa tcgttgaccg caatcgccaa
     9721 gtcatacata aaattgcccc ggcaggcata atagaaatcg atgaagcccg atacctgacc
     9781 gccgtcaagc aacacattgt ctttaaacag gtcggcgtgg atgatgcccg aaggcagatg
     9841 gttgccgaga ttgtccttca gcgcatcgat ttcggaacac agcagcgcgg catcgtcttg
     9901 cgacaggacg ggcagcagcc gggcgcacgc ttccgtccac cacgcatcgt aacgcgggtt
     9961 ttccatttcc aaagggaaat cggcggcggc aaggtgcatt ttcgccaaca tcgcaccggt
    10021 atggaaacac tgctcagccg tcggcagcgc ggtatccgaa cctttcaggc aggcaaccag
    10081 gcaggccggc ttgcccgcca aaacggaatc gagccggccg tctttgcgcg caaccggcgc
    10141 ggcaaccgcc acgcccttca tactcaaatg ccggttaagc tccagaaaaa acgacagctc
    10201 ttcctgtttc aacacttcaa acacggtcag cacataacgt cccgaagtcg tcgtcagaaa
    10261 ataattgctg ttggtaatcc cctgcgcaat gccctgcagg gaaacaaatt cccccaaatc
    10321 gtaaccgctc aggaagccgc gcatttcatc atcggaaaca ctggtataga cagacatatc
    10381 aacttacttg ctcaaaaacg ggcaaacccg ccgtcagaac gccggacggc aggcaaacat
    10441 ataattcaca tcggtcgaat cgcacagggc ataagtttgc gacaacacat ggtaagtcat
    10501 acccttcgta tccgccacat ccaagcccgc ctgacggcac atccgcgcca gctcggcagg
    10561 cgcgatgaat tttttccagt cgtgcgtgcc tttggggacg aacttcaaca gatattccgc
    10621 cgccacaatc agatgcaggt acgatttcgg gtttttattg atggtggaaa aaaacaccat
    10681 gccgtccggc ttgaccagct tggcacaagc acgcacgatg gcggcgggat cggggacgtg
    10741 ttccatcatt tccatgcacg ttaccacatc gaacgagtgc ggttccgcct cggcaaggtc
    10801 ttccacgcgg atacattcgt attcgatatc ggcgacattg ttcaaagccg cgtgcaggcg
    10861 ggcggtttcc agcgactgct ccgccatgtc gatgcccttg acaaacgccg cgccgcgccg
    10921 cgccatgctt tccgcaagga tgccgcctcc gcagcccacg tccaaaaccc gtttgccgca
    10981 caaatccgcg tgtccgtcga tataatcgag ccggagcgga ttgatgtcgt gcaaggtttt
    11041 gaactcgccc gacttgtccc accatttgtc ggcaatccgg ctgaatttgg cgatttcccc
    11101 ctcatcgaca ttatattttt tgtcggacat tttccctccc atctgacgaa ccgcccactc
    11161 caaaacccaa gattatacta tggaacacaa tgccgtctga aagccttttc gggcggcgcg
    11221 gcgcaaatac cttacaaacc cttacacttt acggcataat gcaggctgtt tttcccgagg
    11281 aggcttatat gtccgccaaa actccgtcgc tcttcggcgg cgcgatgatt atcgccggca
    11341 cggtcatcgg cgcaggtatg ctcgccaacc ccaccgccac ctccggcgta tggtttacag
    11401 gctcgctgat tgtgttgctt tacacttggt tttccatgct ctccggcggc ctgatgattt
    11461 tggaagtcaa cacccattat ccgcacggcg caagtttcga cacgatggtc aaagacctgc
    11521 tcggacgcgg ctggaacatc atcaacggca tcgccgtcgc cttcgtttta tacctgctca
    11581 cctacgccta catcttcgtc ggcggcgacc tgaccgccaa aggcatcggc agcgcagtag
    11641 gcggcaaaat ttcgctcacc gtcggacaac tcgtcttctt cggcatcctc gccttttgcg
    11701 tatgggcatc cgcacgcttg gtcgaccgct ttaccggcgt cctcatcggc ggcatggtat
    11761 taacctttat ttgggcaacc ggcggcctgg ttgccgatgc caaaccgtcc gtcctcttcg
    11821 acacccaagc ccccgccggc accggctact ggatttacgc cgccaccgcc ctgcccgtct
    11881 gcctcgcttc cttcggcttc cacggcaacg tttccagcct gctcaaatac tttaaaggcg
    11941 acgcgcccaa agtggcgaaa tccatctggg caggtacgtt ggttgccttg gtaatttacg
    12001 tcctctggca aaccgccatc caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg
    12061 ccgccgaagg gcaagtctcc gtcctgattg aaaccctgtc caaattcgcc caaaccggta
    12121 atatggataa aatattgtcc ctattttcct acatggcaat cgccacctcg tttttaggcg
    12181 taaccttagg cctgttcgac tacatcgccg acatcttcaa atggaacgac agcatgtccg
    12241 gccgcaccaa aaccgccgcg ctgaccttcc tgccgcccct gatttcctgc ctgctcttcc
    12301 ccaccggctt cgttaccgcc atcggctgcg tcggcctggc ggcaaccgtc tggacaggca
    12361 tcatccccgc catgctgctc taccgttcgc gcaaaaaatt cggcgcaggc aaaacctata
    12421 aagtttacgg cggcttgtgg ctgatggttt gggtcttcct tttcggcatc gccaacatcg
    12481 ccgcacaggt attgagccaa atggaactcg tccccgtatt taaaggataa aggcaaaatg
    12541 ccgtttgaaa cccgccggcg gcttcaggcg gcattgccgc aacaaacggc aaccgtattc
    12601 cggcacacag cgcattaccc taccccctca cgcacaaatc ccgccccgac aggcgcggga
    12661 cgcaaccata aaggaacaat gatgaagctc aaaatagaca ttgcaaccaa caacttcaaa
    12721 cacggcggcg gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac
    12781 atcacaccgg ccgtttatgc gacgaaattt gatcacggca ttcccgaata cgccatgatc
    12841 gaaccccatc ttgtcgatca acgccggacg ctgaaaaaac tacgctcgtt cctcttttca
    12901 agccggctcg ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc
    12961 gattacgccg acctcctcat ctgcggcggc acgcacttgg gctacctgca ccatatggcg
    13021 caaaaaccga acctgctcga ccgcctcgcc atacgccgca accgcagcaa ctacgccacc
    13081 gccaaactga ttgtggcgca ttcccatatg atgcggcgcg aactggtcgg actatacggc
    13141 gttccccccg aaagaatcca agtcgccccc ccccccgcag atacggaacg cttctttcca
    13201 caacctagag aaactgccgc cctgcgcgcc aaatacggtt ttgccgacca tgaaaccgtt
    13261 ttcctattcc catcgaccgg ccacacgcgc aaaggcctgg aactgctcgc cgactttttc
    13321 gaacaaaccg aactgcccgt caagctcgcc gttgccggtt caccgcttcc ccgcccgatg
    13381 aaaaacgtcg tcggactggg cttctgcacc gatatgcccg aactctaccg cgccgccgac
    13441 tttaccatta tggcatccct gtacgaaccc ttcgggctgg tcggtgtcga atccgcccta
    13501 tgcggcacac gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa
    13561 gccggattct ttttctcacg acaaaacccg gaaaccctcg cgcaagccgt tgcccaagcc
    13621 gtcagcctta aaaaacaggg cggacaccgc ctgtccgacc cgatgcgggc gctgaactac
    13681 aacccgtccc tgtcgcacca catcgaccga ctgaccgaca tgctggcatc cgtataacaa
    13741 aatgccgtct gaacgggttt tcagacggca ttttcgcaac cgttgcagcc gtgcggcaaa
    13801 aagctaacgg gcaattttca aaacgtcgta cagatagttg cggtaatttc cgaccaactc
    13861 ccggttcggg cagtcttcca accctccgcc gcacaaaccg cgcatcgcca tattcgcacg
    13921 catgaagccg tctttggctt ccaaaccagc cgcctccatc agtacgccgc ctgcaaaggc
    13981 aaggtcgagg aaatcctgcc gctgtacgaa tccgccggca atattgctcc tgacggcaaa
    14041 ctgcgttaca taatccggct gagcgtaatc ccatttcttc ctgacggaca cgccctcaaa
    14101 cggtacctga tgatcgccga aataaccgaa aacaaagggt ttgccgcgtt cgtgcagata
    14161 acggtcgaaa ctttccaccg ctttgtccaa atcggcaatg cgcccgatgt agtcgttaag
    14221 cgcggatacg gttttcgcat tcaaatcggg cgcatccaaa tcaaacacat tgtcggtatc
    14281 cgtccgatac ggcccgtgct ccttcatggt cagcacatat acgaacatcg gctgccgcac
    14341 gttttccaaa tcgggatggc gtttttcgag aatcatccgc gcatactgca tcatttcctc
    14401 actggaaatg tgccacaggt ttttgcccat cggcgcggga tagcccaaat cttgcggctg
    14461 gaacatcaga ttaaagccga aatggtcata tgctgccttg gcattgtagt tgcccttggt
    14521 aaacggcgag agcgccacgc aaaaataacc gtgttcgcgc aggttgcgga caaaaccggt
    14581 ctgcaaatgc ggtacgaccg aataaaacac gccgcttgcc aacgcgccga aatctgtgga
    14641 cggaacaccc gccaaaaacg caaattcgga cttccacgtt gcgccgccga aagtatgcac
    14701 gcgcaaaggt gaggaaaata cagtatcttc ctgccgtccg aacattttca aatcgggaat
    14761 tttggcggcg gcaaaatcga aacagtgcgg atcgagcgtc gattccatca gggtaacgac
    14821 aatatcgggc ttttcatccg acatgccgta cggctgggtt tcagacggca tctgcctggc
    14881 aaacgcttcc ccgtcgccct cgaacacggg tacttggaaa aacaccgccc gacaggacat
    14941 cggcaggttc aaaaacacgt cgcgcccgtc gtccggcagc gagtccagcc acgtctttac
    15001 cgcgccgggg tgcttggaaa aatgccgcac cgacacgaac gccgccgcaa acagaaccgc
    15061 gcccgcccaa cgccacggca tacccaaaga atccgcaccg ctccagccga aaaccgcata
    15121 tgccgccaaa gccagcagcc ccgccatacc gataaccgct tccttataat gaaacagcgt
    15181 ttcccaattc cgccagtcgg caatcagcaa aaagtcggaa atcaacagcg gctgtttgta
    15241 ataatcgatt ttcagcctgt ggaacagtat cagcaccaca aaccagaccg aagcgaaatt
    15301 caaggcgcgc tgccactgcg ccgtcaacat cagcataccg cccgccaaaa agacaaacag
    15361 cgccgacgcg aaaaaatacg tccagcggta gtgcgacctg acaatcagca gcaccaccgc
    15421 cgttacaaac aaaaatagga aagcataggc aaccatcccg ccccactcct caaaacggca
    15481 tacctgtgcc gttgcgaatt attatcgaaa gcatgcatta taatgcagca caggcgcatt
    15541 tccaccgata tttcagtata atgccatccc cgaacccgcc caacccaaag gaaccgtgat
    15601 gaaactcatc attctcgacc gcgacggcgt catcaaccgg gaccgcgacg acttcgtcaa
    15661 atccgttgac gagtggatac ctgtcgaagg cagcatggat gcggtggcat tcctgacgca
    15721 ggcaggctgc accgttgccg tcgccaccaa ccaatccggc atcggccgca aatattttac
    15781 cgttcaagac ctcaccgaaa tgcatgccaa aatgcaccgc ctcatccgtc aggcgggcgg
    15841 ggaaatcgac ggcatctggt tctgcccgca taccggcgcc gacggctgca actgccgcaa
    15901 gcccaaaccg ggcatgattg aagacatcct cgaacgcttc aacgcccaag cttcggaaac
    15961 ttggctggtc ggcgacagcc tgcgcgattt gcaggcaatc gatgccgtcg gcggaaaacc
    16021 cgcgctggtt ctgaccggaa aaggcaaaaa aacgctctcc caacacggac acgaattacc
    16081 cggacacaca caggttttcg ataccctgct cgatttctca caatacatca tgcaggaaaa
    16141 cgccgcaccg caagccgact gaacataccg catttccgac aaggcaaaac catgctcatc
    16201 atccgcaacc tgatttactg gctgatactc tgttccagcc tgattttcct ctttcccttt
    16261 atgctgctcg cctcgccttt ccgggacggg gcgcacaaga tggcgcgggt ctgggtcggc
    16321 atcctcaact ggtcgctcaa acacatcgtc gggctcaaat accgcatcat cggcgcggaa
    16381 cacattccgg accgcccctc cgtcatctgc gccaaacacc aaagcggctg ggaaacgctc
    16441 gcgctccaag agatttttcc gccgcaggtt tacgttgcca agcgcgagtt gttcaaaatc
    16501 ccctttttcg gctggggctt gaaactggtc aaaaccatag gcatagaccg caacaaccgc
    16561 cgcgaagcca acgaacagct cataaaacag ggtttggcgc gcaaaaacga aggttattgg
    16621 attaccattt tccccgaagg cacgcgcctt gcgcccggaa aacgcggcaa atacaaactc
    16681 ggcggcgcgc gcatggcgaa aatgtttgag atggacatcg tccccgtcgc cctcaacagc
    16741 ggcgaatttt ggccgaaaaa ttcctttctg aaatatccgg gggaaatcac cgtcatcatc
    16801 tgtccgacca tcccgcacgc aagcggcagc gaagccgaat tgatggaaaa atgcgaacat
    16861 ctcatcgaaa cgcagcagcc gctcatttcc ggcgcaggcc cgtttgccgc cgaaatgccg
    16921 tctgaaaccg catgacctcc ctgattcaca ccctttcaga cggcatagaa ctgaccgtcg
    16981 aaatcaaccg ccgtgccaag aaaaacctga ttatccgccc tatcggcaca cataccgtcc
    17041 gcatcagcgt cccaccctgc ttctccgtct ccgctctaaa ccgctggctg tatgaaaacg
    17101 aagccgtcct gcggcggaca ctggcgaaaa caccgccgca caatacggca aaccgactgc
    17161 ccgaacatat ctggttccac ggcaggcagc ttgcccttac cgcccatcaa gacacgcaaa
    17221 tcctgctgat gccgtctgaa atcctcgccc ccgaaggcgc gcccgaaaaa cagcttgcgc
    17281 tgctgcggga ctttttggaa cggcaggcgc acagttacct gattccccgt ctcgaacacc
    17341 acgcccgcgc cacgcaactg ttccccgcct cctcctcgct gacctccgcc aaaaccttct
    17401 ggggcgtgtg ccgcaaaacc acaggcatac gcctcaaccg gcggctggtc ggcgcacccg
    17461 aatacgttgc cgactatgtc tgcatacacg aactctgcca cctcgcccat cccgaccaca
    17521 gccccgcctt ttgggaactg acccgccgct tcgcccccta cacgcccgaa gcaaaacagc
    17581 ggatcaaaat ccacggcagg gagcttttcg ccttgggctg acgcagcccc gggccgacgc
    17641 tttcagacgg catccgtgcc ggaacaggca cgcgcccgcc cgattcaaac cgcgatgacg
    17701 ctttgccgcc ggttcggggc aggatggcgg catccgcgcc gtccgcaccg tttcatttca
    17761 caccgccctt ccgaaacccg cccgatccga cgtgcggtat gaaacgcttg agccggcgcg
    17821 aagccttcta ctgatttgcc cgcgaaaata ccgtccgaaa ggttttcgga cggcattttt
    17881 ttgcgttttc ctggatggcg gcggattcgt aaaaggcggg cagggtggat tgcaggatgg
    17941 gttgaggcct gccaaatccg ccaactctac cgaatacgcc gccgtcattc ccgcgcaggc
    18001 gggaatttgg ttttgtcggt gcggaaactt atcggataaa acggtttctt agattccacg
//
LOCUS       DAXDZG010000047        17949 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXDZG010000047 DAXDZG010000000
VERSION     DAXDZG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17949
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.047"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(51..1472)
                     /locus_tag="ACFB78_001345"
     CDS             complement(51..1472)
                     /locus_tag="ACFB78_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC1269323.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHYHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1831..2190)
                     /locus_tag="ACFB78_001346"
     CDS             complement(1831..2190)
                     /locus_tag="ACFB78_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC1269324.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2187..2792)
                     /locus_tag="ACFB78_001347"
     CDS             complement(2187..2792)
                     /locus_tag="ACFB78_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC1269325.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2839..3321)
                     /locus_tag="ACFB78_001348"
     CDS             complement(2839..3321)
                     /locus_tag="ACFB78_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC1269326.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3525..3622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3833..4933
                     /gene="gcvT"
                     /locus_tag="ACFB78_001349"
     CDS             3833..4933
                     /gene="gcvT"
                     /locus_tag="ACFB78_001349"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC1269327.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKVLQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5080..5619
                     /locus_tag="ACFB78_001350"
     CDS             5080..5619
                     /locus_tag="ACFB78_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269328.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5671..6057
                     /gene="gcvH"
                     /locus_tag="ACFB78_001351"
     CDS             5671..6057
                     /gene="gcvH"
                     /locus_tag="ACFB78_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC1269329.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6210..7457
                     /gene="hemA"
                     /locus_tag="ACFB78_001352"
     CDS             6210..7457
                     /gene="hemA"
                     /locus_tag="ACFB78_001352"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC1269330.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7767..10009
                     /locus_tag="ACFB78_001353"
                     /pseudo
     CDS             7767..10009
                     /locus_tag="ACFB78_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449380.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB78_001354"
                     /pseudo
     CDS             10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB78_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12390..12545
                     /locus_tag="ACFB78_001355"
                     /pseudo
     CDS             12390..12545
                     /locus_tag="ACFB78_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12542..13633
                     /locus_tag="ACFB78_001356"
                     /pseudo
     CDS             12542..13633
                     /locus_tag="ACFB78_001356"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13691..14587
                     /locus_tag="ACFB78_001357"
     CDS             13691..14587
                     /locus_tag="ACFB78_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1269331.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14584..15414
                     /locus_tag="ACFB78_001358"
     CDS             14584..15414
                     /locus_tag="ACFB78_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1269332.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15411..15905
                     /locus_tag="ACFB78_001359"
     CDS             15411..15905
                     /locus_tag="ACFB78_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC1269333.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16039..17700)
                     /locus_tag="ACFB78_001360"
     CDS             complement(16039..17700)
                     /locus_tag="ACFB78_001360"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC1269334.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4346 a         4636 c         4865 g         4102 t
ORIGIN      
        1 gattatgccg gaatttaaaa tgccgtctga atgttcagac ggcatagcgt ttacagtagt
       61 ttgaaaacga aaaagacgag ggtatgtacg atgaagacgg gtgtcaggaa ggcgaccgac
      121 cacatcatat agccgaagaa agtcggcatc ggtacgccgc gctgttcggc aatggccttg
      181 accatgaagt tcggtgcgtt gccgatgtag gtcagtgcgc ccatgaatac cgaacccata
      241 gaaaccgcca gcagcgaatg aaacagggga cccgtcatta aggcttgggc atcgccgccc
      301 gccatattga aaaacacgag ataagtgggc gcgttatcca agaatgccga caatatgccg
      361 ctcatccaga aatacatcgt attaatcgga tgacctgccg tatcgtgaac cagcgatacc
      421 accccgccca gcgcgcctgc ctcgcctgct ttcagaatgc tcaggacggg gaagatggtg
      481 atgaagatgc cgaggaagag tttgcccact tcggcgatgg gttcaaagtt gaattcgttg
      541 cctgcgcgga cttgtttggg cgtgattgcc atagatacgg cggtcaatgt aatcaggatg
      601 acatcgcgga caaggttttg cagggcgtaa cggctgccga ggatttcaaa tcccgggtgt
      661 tcgggtttcc aaaggccgga cattagaacc gcgccgacca cgcccgaaag caggaggaag
      721 ttccatttgc cgaagatggc gattttttcg ggtttttcct gttgtgccgg cgtatcttgt
      781 gcaatgcttt cctgtttgaa gaaacggttg tcgatgaaat agaaggcggt caacaggaca
      841 gcggtgctaa tcaggacggg ggcgaacata tgtttgaccg tccacatgaa atctacgcct
      901 ttgaggaagc cgaggaagag tggggggtcg cccaaagggg tcaggccgcc gccgatgttt
      961 gcaaccagga aaatgaagaa gatgacgatg tgcacgcggc gggtacggtc ttggttggct
     1021 ttcagcagcg gacgaatcat cagcattgcc gcgccggtcg ttcccatgat agaggcaagt
     1081 gccgtaccga cggcaagcag ggcggtgttg agcttgggtg tgccgttcaa gtcgccccaa
     1141 accaaaatgc cgcctgaaat ggtgtacagg gcaagcagca gcaggatgaa ggggatatat
     1201 tcttcaacga gtgcgtgtgc gacggtatgg ataccggcgg acgcgccaaa aaccaaactg
     1261 aacgggatga ggaagagcaa tgtccaaaag gcggtgattt tgccgtaatg gtaatgccag
     1321 gtatgcgaaa aaaacaaggg acccaatgcg atagacagca aaatcagggc aaagggcaag
     1381 ccccacagca ggtttaggtt tgcgccgtcc gaatctgcgg cgtaaaccga tgccgggaaa
     1441 agcattagtg aaaacagggg taggtggcgc atcgtgtttc ctcgattcaa gcactgcctt
     1501 gcgcggcgcg cgggagtgat acaggcttcg tgccgcccgg acataggcgg actgtgctgc
     1561 ctgtttgttt ttgaaaaaag ttttccgatt gattgtaaag taaataatca ggataaacca
     1621 gtttcccaaa ccggaaggcg gcgggaaggc ggattgccgt gcttgggaat atatgttctt
     1681 tttgataaat aattttattt aaaacaaata aatatatgtc tttaataaat aatctgtcga
     1741 aaacgaaaaa taaatttatt ttaacatata tttgcaatga aacaggtttg ccccacccgt
     1801 ttgtttgccc ttatcccttt cagacggcat tcaagattcg ggtctgcgcc acatccatat
     1861 ggcgacaagg gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt ggggaaactg
     1921 ccaaaacatc atcaggcagg atgcggtcat catgctgatg gcgaaaatct tggctttgcg
     1981 cggcactgcg ccgttttgtt cccagttatg aaccatcggg ccgaaatagc ggtgccggtg
     2041 cagccagcgg tgaaagcgcg gggatgcctt tgcccagcag gcggcggaga gtagtacgaa
     2101 cggcgtggtc ggcaacagcg gcaaaaaaat gccgatgatc cccagcagca gggagatgcc
     2161 gccgcaggca attaaaagat aacggatcat tttgaaatat ttttcttatt gtgcggataa
     2221 gggcaggatg tgataccgag ttttgcccag ccttcatgtc ccattttttc cagcagggcg
     2281 atattgcgtt cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt ggcaatgctg
     2341 tcttcgcgga tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt gccgatgccg
     2401 tctgaatccg tgccttcaaa ttggaaatag ggatgaaacg gggcgatttg gacgatgcct
     2461 tccaagccgt tttcgacaac ggcggcatcg gcaatgtcga gcatatcgtt gaatacgtcg
     2521 aaatcgggga atagggtcgg gtgaaccagc agggtggttt ccagttcggt ggcgggtgta
     2581 ttgcccagtc gctgcagttc ttcgtccaag tcttccaaaa aaccgtcaag gtgtttggct
     2641 tcgctgatcg cgatgcggac aaggttttta acgtgggggg ctttggcaaa gggacacagg
     2701 ttcagaccga tgacggcttt ttccaaccat tgtccggtgt gttcggcaac agcatcttta
     2761 ttttcggaag tatcgatatt cattattgtc atgtaaatgt gtttgcagag tgcacgtgcg
     2821 ggaaaatcgg gaagggcact attccttcag caggtggttg agcggcaggg aggtggtgtg
     2881 tttgatttct tttaaaacaa agctcgattg cgcatcctgt acgccgtggt gggataaaag
     2941 cgtatccaaa acaaaatggg aaaacgcgtt catatcggta aaaaatgcct gaagcaggta
     3001 gtcggtctcg ccggttaggg cgaagcagct caagacttcc ggccattttc gaaccgatgc
     3061 ggcaaaatct tcccgcgcgt cttttgcttt gcggatggaa acgcggataa atgcctgaag
     3121 acctaagttg acggattccg gagacagcag cgcggcatat tggcggacga tgccggcatc
     3181 ttccaactgc ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac gttcggacag
     3241 ttcgacattg gtcagcctgc cgttttcctg gaggacctgt aagattttaa tatcggtttt
     3301 gtctaaagtg agttggggca tatttgcgtt ccgttttaag gaattcggat tgtctgtccg
     3361 tatgtttgcg gcaatccgca tagatggaga ccatattaac atataaaaag ttataccgtc
     3421 atccgggaca aattttgttt tcggaaaatc atgtgaaaac agaggcggtc ggtttgcatc
     3481 tcttttaaga cggcttgccc aaaccgccga ttcaagacat aatcgggaaa tgtgcaggag
     3541 agtgttacac ccaactacaa tgtaaccacc gaaggcgcag acacccttaa atcgctcagg
     3601 tatcagggac tgcacattga aacaaacaat ctggagagcg gcgttagaat aacgtccacc
     3661 gaaggggaga aggccgtctg aaccaccatt cagacaaccg cgcaaagcag tgagcagact
     3721 ggtttgccat catgcggata cggccgaaaa tctcaggttc aaggacagat agggtcatcc
     3781 gcgcacaggt gcgcgggcgg catctgaaca aaaaatccgg agaaacttga gaatgactgc
     3841 tctgaaaacc accccgtttc atcaagccca tcaagatgca ggcgcgaagc cggtagattt
     3901 tgccggctgg gaactgccca tccattatgg ttcgcaaatc gccgaacacg aagccgtgcg
     3961 caccgacgcc ggtatgtttg acgtatccca tatgctcgtt accgacgtcg tcggagcgaa
     4021 cgccaaagcc tttttccgca aattgattgc caacgacgtt gccaaactcg cttttgtcgg
     4081 caaagccctt tattccgctt tgctcaacga caacggcggc gtgattgacg acttgatcgt
     4141 ttaccgcacc aacgaggccg aaacccaata ccgcatcgtg tccaacggtg cgacccgtga
     4201 aaaagacacg gcacaattcc acaaagtcgg acaagagttc ggcgttgcct tcaatccgcg
     4261 ctacgacctc ggtatgcttg ccgtacaagg tcctaaagcc attgaaaaac tcctgaccgt
     4321 caaacccgaa tgggcggatg tgattcacgg cctcaaaccg ttccaaggcg cggatttggg
     4381 caacgactgg tttgtcgccc gtacaggcta caccggcgaa gacggcgtcg aagtgattct
     4441 gccggatacc gaagccgtcg cattcttcaa agtcctgcaa acagcaggcg tacagccttg
     4501 cggcctcggc gcgcgcgaca ccctgcgcat ggaagccggt atgaacctct acggcaacga
     4561 tatggacgac gacaccagcc cgctcgaagc aggcatgggc cggacggttg atttgaaaga
     4621 cgaaagccgc gattttgtcg gtaaagccgc cttgttggca ttgaaagaaa aaggcgttgc
     4681 cgtcaaacaa gtcggtttgc tgctcgaaaa aggcggcatc ctgcgcgcgc atatggaagt
     4741 gttgaccgac aaaggaaaag gcgaaaccac cagcggcgta ttctcgccca gcctgaaaca
     4801 atctatcgcc atcgcccgcg taccgaaaga ttttgacggc gataccgcca aagtcctgat
     4861 tcgcggcaaa gaagcggacg tgcgcgtact gaagctgccg ttcgtccgca gcggtcaaaa
     4921 acagtttgat taatatcggt tcagacggca tttgtttgaa ttaagccgtc tgaaagcagg
     4981 ttttaaggaa ttgttatttc ctatgaaagc aaattttggc agtttattgt tgttattgat
     5041 gttatcggct tgcgcttcgt cgccggcgag cgcggattga tgtttttcga tacagaagga
     5101 aaatcccata ctgcaatatt tatgaaaatc gttggcggca atggaaagat tgccggcgag
     5161 gtggtgatag acggggaata tgctcttgat attgtccgca tgaatgatga tttgagtatt
     5221 gccgtgcaag ataataaggg tagaagccgt gcttccgacc tggaagcatt gtcgcaagcg
     5281 aaatcattta ccctaattgc gttcagccgt tatgcgggca cagttgaagt ggataaagta
     5341 tcggctgtta caggcatttg tgcagattac cgcagcaaag gcctgaaggt tgccgttgca
     5401 gacaatatga ggcccaaaca agaaatcgtt gtattccgtg caagcggcgt aatggcaagt
     5461 aataagcgca agataaacga agcatatttg gattattctg catcctctaa actgccttcc
     5521 gcccaaaagc aagaattgga acaagataaa aacaatatga ttgccgtcca ttacggacgc
     5581 ttggaagaat taatcgcacg aggtatctgc gtacgctaag ttgtgcagat gccgtctgaa
     5641 aataaccccc taccaatgga gaatcaaacc atgagcaaca acatcccaac agaactgaaa
     5701 tacgttgcca gccatgaatg gctgcgcctt gaagaagacg gtatcattac cgtcggtatt
     5761 acccatcacg cgcaagagct gttgggcgac atcgtgtttg tcgagctgcc cgaagtcggt
     5821 gcgaacctcg ccgccgaaga acagtccggt gtggttgagt ctgtaaaagc cgcatccgac
     5881 gtgtatgcgc cgattgcggg cgaagtcgtt gccgtcaacg atgacttgcc aggcgcgccg
     5941 gaaaccgcca acagcgaccc ttacggcgca ggctggttct tcaaaatcaa acctgccaat
     6001 cctgccgatt acgacggtct gctgactgcc gaacaatacg cgggcgaagt ggattaaacc
     6061 gcccgtttgt ccgacggcaa ccgtcggaca aacggaaact gcaccttcag acggcatttc
     6121 tgcggtcgga ggtgcagttt ttttgtccgt gttttaagga tgcagttagg ctataataac
     6181 ggtctatatc catcttcacc gattttttca tgcaacttac cgctgtcgga ctcaatcatc
     6241 aaaccgcacc tttaagcata cgggaaaagc tggcgtttgc cgccgccgcc ctgccagaag
     6301 ccgtccgcaa tcttgcccga agcaatgcgg caacggaggc ggtaatcctt tctacctgca
     6361 accgcaccga gctttactgc gtcggcgatt cggaagaaat catccgatgg cttgccgatt
     6421 accacagttt gccgattgaa gaaatccgtc cgtatctgta cacgctggat atgcaggaaa
     6481 ccgtgcgcca cgccttccgc gttgcctgcg gcttggattc gatggttttg ggcgagccgc
     6541 agattttggg gcagattaaa gatgcggtgc gtgcggctca agaacaggaa agtatggggg
     6601 caaaactcaa tgccctgttc caaaaaacct tttccgttgc taaagaagtc cgtaccgata
     6661 ccgctgtcgg cgaaaattcg gtttcgatgg cttccgcgtc cgtcaagttg gcggaacaga
     6721 tttttcccga catcggcgat ttgaacgtat tgtttatcgg cgcaggcgaa atgattgagc
     6781 tggttgccac ttattttgcc gccaaaaatc cccggctgat gacggttgcc aaccggacgc
     6841 tggcgcgtgc acaggagttg tgcgacaagc tcggtgttaa cgcggaaccg tgcctgctgt
     6901 ccgatctgcc tgccattctg cacgattacg acgtggtggt ttcttcaacg gcgagccagc
     6961 ttccgatagt cggcaaaggc atggtcgaac gcgcattgaa acagcgtcag agtatgccgt
     7021 tgttcatgct tgacttggcc gtgccgcgcg atattgaagc ggaagtcggc gatttgaacg
     7081 atgcgtatct ttatacggtg gacgatatgg tcaacatcgt ccaaagcggc aaggaggcaa
     7141 ggcagaaggc cgccgccgcc gccgaaacgc tggtgtccga aaaggttgcc gaatttgtca
     7201 ggcagcagca gggcaggcag agcgttccgc tgattaaggc cttgcgggac gagggcgaga
     7261 aagcgcgcaa gcaggtgttg gaaaatgcga tgaaacagct tgccaaaggc gcaacggcgg
     7321 aagaggtttt ggaacggctg tccgtccaac tgaccaacaa gctgctgcat tcgccaactc
     7381 aaaccttgaa taaggcgggg gaagaagata aagatttggt tcatgccgtc gcgcagattt
     7441 atcatttgga caaataacgg tgcgccggaa aaggatgccg tctgaagggg tttcagacgg
     7501 catatttttg tgccgcctga caacatcgtg aaatcccaca ttatatcgat gtaattacaa
     7561 agtattatcc tatatgcgga ttggttttaa gatttgtaaa tttgatttgc atcaaaaatc
     7621 gccgatagat gattcatata atatcaatat taaagagtat cggtatatcg gggatagcta
     7681 tgtcctgttt ttcaatccgg cgtatgtctg cgtttcgggc gcggataacg gcgtttttta
     7741 ccgcctttgt ctttttgacg gcggcactgc ccgcttatgc ggagcgtctg cctgattttc
     7801 tggcgaaaat acagccttcg gaaatttttc cgggtgcgga tcgttacggc aagccggaag
     7861 gcaagcctat ggttgcccgc gtttacaaag gcgatgagca gctcggtttg gtttatatca
     7921 cgaccgatgc ggtcaatacg cgcggttatt cgagcaaacc gatcgatacg ctgatggctt
     7981 tggcaaacga cggcacgata gccggggcga aactggtcga tcatcacgaa ccgattatgc
     8041 tgatcggtat cccgcaatcg cgtgtcgata agttcatcga caaatatatc ggtctgaatt
     8101 ttattaaaaa tccgccgacc ccgggcgtgg cgccgggcga catcatcagc ggtgcgactg
     8161 ttacactgat ggtggttaac gacagcatcc agcgttcgta caaggtcatt gccaaccaat
     8221 accgtctggg ttcggacaag gcccttcaga cggcatccgc ttccgatgtt cgggaagccg
     8281 cgcctgcgtc agaaacccgt ccgcgccgta tggcaaatcc cgacaagcag gatattttgt
     8341 cttgggacga acttttgaaa caaaaggccg tcggccatct gcatatcacg ctcgatcaaa
     8401 tcaacaaact gtttgagaaa ggcggcaagg ccggcgtggc cgatcacgcc gaacagggcg
     8461 atcctgacga tacctttatt gatttgtatg ttgccttggt cagccagcct tccatcggta
     8521 aaagcctgct gggtgaggac ggctgggcgc atctgcaaaa acggctgaaa cccgggcagc
     8581 aggcggtttt ggttgccgga gagggccgtt attcttggaa aggttcgggc tatgtgcgcg
     8641 gcggtatttt cgaccgtatc gagatgattc agggggagaa cagcttccgt tttaccgatg
     8701 cccaacacga acgcgtcgtc gagctgtctg ccgccgatgc gccgcgtttt aaagaagttt
     8761 cttggtttac catccctgaa ggcgtagcgt ttgacggtgc ggagccgtgg cggctgtaat
     8821 tgatggttca gcgcgtcttg agtgtgaacg ataaagcctt cgttaccgcc gatttggatt
     8881 acgaactgcc ccaagcttat tacgtcgatg atcctaaagc gccgcccgta gaaatcagcg
     8941 cgcctgtcga agcagtacct gccgcagcat ccgataccgc ttcagacggc attgccgaag
     9001 atgcgtcagc tgaaaacggt gtatccaacc aattatggaa acagatttgg aaagcgaaac
     9061 aggggcagat tgtcgtcgtc ggcattgcgc tgaccatcct gcttttggta ttcctgttcc
     9121 aagattggat tgtgcgttac gaaaaatggt acgaccgctt ccgctttgct ttcctaactt
     9181 ttacgctctt ctatatcggc tggtatgcac aggcgcagct ttcggtcgtc aatacgctga
     9241 cgctgttttc cgccatcctt accgaatttc attgggaatt cttcctgatg gatccgattg
     9301 tgttcatcct gtggctgttc acggctgcaa ccatgctgct gtggaacagg gggacgtttt
     9361 gcggttggtt gtgtcggttc ggctcattac aggaattgac taaccgcatt gccaaaaaac
     9421 tgggtgtgaa acagattacc gtaccgcata tgctgcatac ccgtttgaac gtcataaaat
     9481 acctgatttt attcggtttt ttggcaattt cgctgtatga tttgggtacg gcggaaaaat
     9541 ttgccgaagt cgaaccgttc aaaactgcga ttatcttgaa attcatgtgc gattggtggt
     9601 ttgtcgcctt tgccgtggca cttttgattg cggggctgtt tatcgaacgc ttcttctgcc
     9661 gttatctgtg tcctttgggt gcgggcatcg cgctgccggg cagattccgc gtgttcgact
     9721 ggctgcgccg ctacaaaatg tgcggcaacc cctgccaaat ctgtacgcac gaatgccccg
     9781 ttcaggcaat cgcgcccgag ggcgacatcc atccgaacga atgtatccag tgcctgcact
     9841 gccaagtgat gtaccaccac gatacgcgct gtccgcaagt tgtggcggaa aataagaaaa
     9901 acaaaaacag gctgctgcca aatcagggga attggaaaat gtctctaagc agccgcagga
     9961 acaggtcgtc cgttttgtca aacccgaaac ggcgcaatct gaaaaataag catccggggg
    10021 aagcctgaag ccaaagtgtc aggcaggcag ggtttccccg gaaggataat aaaatttttt
    10081 tcccatcggc ttgccgtccg aaaaccggtt tgccgtattt tatccaagga gtgttgtatg
    10141 tcagacgaaa aattagaaca aaacggcttg agccgtcgtt cgttcttagg tacggccgcc
    10201 gcttccggtg caggtattgc cggtgcgggc ctgttgggtt tggcgggttg ctctaaagac
    10261 ggcgaacagg ctgccgctaa cgcttccggc gcggctcccg tcgccaaggc gcaaggggaa
    10321 tccaaacccg gccaactgtc ttccgaagtc ggtccgggcg aactcgatca atattacggt
    10381 ttcctttccg gcggccagtc cggcgagatg cgcctgatcg gtctgccttc tatgcgcgaa
    10441 ctgatgcgta tccccgtgtt caatatggac agcgcgaccg gttggggacg caccaatgag
    10501 agcttgaaag tcctcaacgg caatattacc gaagaaaccc gtaaattctt aaaagacggc
    10561 ggcctgcgct gctaccccaa cggcgacttg caccacccgc acctgtcgtt taccgaccaa
    10621 acttatgacg gccgctatgc ctatgccaac gacaaggcaa acaaccgcgt ctgccgcgtg
    10681 cgtttggatg tgatgaaggc cgacaaaatc atcgacatcc ctaatgattc aggtattcac
    10741 ggtctgcgtc cgcaacgtta tccgaaaacc ggttatgttt ttgccaacgg cgaacacatt
    10801 acccctgtca gcggtgtagg caaactggat gatgcgaaaa cctggaatgc agtgtacacc
    10861 gccatcgacg gcgaaaccat ggagatcgca tggcaagtat tggttgacgg taacctggac
    10921 aacggcgatg ccgactatca aggcaaatat tcttttgcca cctgctacaa ctccgagcgc
    10981 gcgctgaccg tacaaggtgc gtcttccaac gagcaggact ggtgcgtcgt tttcgacctg
    11041 aaagccatcg aagaaggcat caaagcgggc gacttcaaag aagttaacgg tgtgaaaatg
    11101 ctggacggcc gcgctgaggc caaatccaaa tacacgcgtt atatccctgt gccgaactct
    11161 cctcacggct gtaacgcaag ccctgacggt aaatacatca tgcccaacgg taaactgtct
    11221 ccaaccgtta ccgtattgga tgtcagcaaa ctggacgatt tgttcgccgg caaaatcaaa
    11281 gagcgcgatg tggtcgtagc cgaaccgcaa ctgggtctcg gcccgttaca cactgcattc
    11341 gacggtcgcg gcaatgctta tacgacattg tttatcgaca gccaaatggt gaaatggaat
    11401 attgacgatg cgatcaaagc ctacaaaggc gataaaatcg atccgatcaa acaaaaactc
    11461 gacgttcact atcaaccggg tcacaaccat acgaccatgg gcgaaaccaa agaagccgac
    11521 ggtcaatggc tggtgtcctt gaacaaattc tccaaagacc gcttcttgaa tgccggtcct
    11581 ttgaaacccg agtgcgacca attgatcggc atctccggcg acgaaatgcg tctggtacac
    11641 gacaacccga cttttgccga accgcacgac ttgtgtttgg ttgccgcgtc caaactgaac
    11701 ccgggcaaaa cttgggaccg caaagacccg tggttctagc aggatgcttt ggaacaggcg
    11761 aaaaaagacg gtgtcgagct ggaaaaagcc gccaaagccg tacgcgaagg caacaaagta
    11821 cgcgtgtata tgacggccgt cgcgcctgct tacagtatcc cgcagtttga agtgaaccaa
    11881 ggcgacgaag taaccgttta cgttaccaac gtcgaaacca tcgaggacct gacccacggc
    11941 ttcactttgg aaggctgcgg catcgctatg gaaatcggcc cgcaagccac ttcttccgta
    12001 accttcaaag ccgtccgccc gggtgtgcac tggtactact gccaatggtt ctgccacgca
    12061 ttgcatatgg aaatgtccgg tcagatgatt gttcatccca aataatgcgg cccgacggct
    12121 gaatatggag aatggcgtct gaaggcaaca ggtcttcaga cggcatttat agccaaaccg
    12181 cttgccgtac cgtttcaggc ggtttcgcta taaaaacagg cacatttgaa tgatgcttta
    12241 ttttgttcgg ccataccgaa cgaaacagag ggacactatg cacacatccg cgctccgtat
    12301 atggctgaag gccgtgctga tacttgccgc cggcagcatt tttcagacgg catcggcagc
    12361 cgtcgtccac gtttccccgc aagacaacct tgccgaaatt tttgcccgcg cccgggcggg
    12421 cgatacgatc aaactggctt ccggcgtata tcaaaccaaa ctttatatcg acaaaccgat
    12481 tacgattgaa gggcctgccg accgttccgc aaccatcgaa ggggacaaga gcgggcgtac
    12541 catagccgta cacgcgccgg acgtaacgct ccgcaacctg accgttaccc gttccggtat
    12601 gagcctgcca gcaatggatg ccggtattta tctcgaaaaa gccgccccgc gcgccctgat
    12661 tgaacacaac aatatttttg ataattcgtt cggcgtatac cttcatggtt ctgccgatgc
    12721 gatggtgcgc gagaataaaa tcgtcggcga tgcgaccttg cgcgtgaatg agcgcggcaa
    12781 tggcgttacc gtttggaacg cgcccggcgc gcaggtcgtc ggcaacgaca tttccaaagg
    12841 gcgggacggc attttttcca ataccagcac gcacaacacc tataaaaaca accgcttcag
    12901 cgacctgcgt ttcgccgtcc actatatgta caccaacgac agcgaagtca gcggcaatat
    12961 ttgcgtgggc aacaatatgg gctatgtgct gatgttttcc gaacggctca aagtgttcga
    13021 caatatcgcc gtcggcagcc gcgattaggg catcatgctc aactatgtca actattccga
    13081 tattcacgac aatattatca acaaagcggg caagtgcgtt tttgcctaca atgccaacta
    13141 cgataaactg tccgccaatc attttgaaaa ctgccaaatc ggcatgcact ttaccgccgc
    13201 catcgaaggc gcgtccctgc acgacaattc ctttatcaac aacggaagcc aggtcaaata
    13261 tgtcagtacg cgctttctcg actggagcga gggcggacac ggcaactact ggagcgacaa
    13321 cagcccgttc gatttgaacg gcgacggctt cggagacagc gcgtaccgtc ccgacggcat
    13381 catcgaccaa atcatctggc gcgcgcccgt atcgcgcctc ttgatgaaca gtcccgcaat
    13441 cagcatcgtc aaatgggcgc aggcgcagtt tcccgccgtt ctgcccggcg gcgtggtgga
    13501 cagcaaaccg ctgatgaagc cttatgcccc caaaattcaa acccgttatc aggcgatgaa
    13561 ggacgagttg ctcaaagaag ccgaaacgcg gcagtcggaa cggggcaggg cggaaaacgg
    13621 ttctttgaac tagtctgctt cagacggcac ccggattcaa atgccgtctg aaaacacaaa
    13681 aggaacaacc atgaccacac atcatgtcga attgaggaag gtaaccaaac ggttcggggc
    13741 gcaaaaagcc gtcagccaag tcgatttggt tttgaaggca ggagaaagcg tcgggcttgc
    13801 cggacacaac ggcgcgggca agtccaccat catgaagctg atactcgggc tgattacccc
    13861 gaccgaaggc gaagtgatgc ttttgggcga acgtaccggc agcaaagcgg gggcgcggct
    13921 tcgcagccaa atcggctacc tgcccgaaac cgttgcgctg cacccttcgc tgaccggcat
    13981 cgaaacgctg gatttttatg ccaaacttaa aaaacagccg ctcacgcaga accgggggct
    14041 gcttgagcgc gtcggcattt cccagaccgc acaccgccgc gtcggcactt attctaaagg
    14101 gatgcgccaa cgccttgcct tggcacaagc cctgctgggc gagcccaaag tcctgctgtt
    14161 tgacgaaccg acaaccggcc ccgaccctgc atcacgacaa atgttttacg aagtcgtgcg
    14221 cgaactcaac gggcgcggcg cgaccgtatt gctcagtacc cacgcccttg ccgagttgga
    14281 cggacacgcc gaccgcattg tcgtgatgaa aaacggcgtt aaggttgccg acggcagtat
    14341 ggacgaattg cacgtccaaa gcgggctgcc gctgaccgtc aatatccgcc tcaatgcgcc
    14401 gcgcaccctc agcagccgct ggcagccgct ttcagacggc atttcctacc gggcgcaatg
    14461 tcaggccgaa gaacgtatgg aactgttggg cgaattgggc agcctgtccg accttgccta
    14521 tttcgacatc cacacgccga cgcttgacga aatgtacgca cggttcttga aaagggagga
    14581 cgtatgaacc ccgtttggat tattaccggc aaagaagccc gcgacagcct gcgtaaccgc
    14641 tgggtgcttg ccgccgtcct cctgcttgcc gcactcgccc tttctttggg tttcctcggc
    14701 agctcgccca ccggttcggt caaggtcgat ccgctgaccg ttaccgtcgt cagcctgtcg
    14761 agcctgtcta ttttcctgat tccgctgatt gcgatgctgc tttcttatga cgcactgatc
    14821 ggcgaaatcg aacgcggtac gatggcgttg ctgttgagtt atcccatttg gcgcaaccaa
    14881 atccttgccg gcaagtttgt cggacacctc atcatcctcg cccttgccac cacggcaggt
    14941 tacggattgg caggcattac gctgcaactt gccaacggcg gtttcgacat cgccgtttgg
    15001 aaaccctttg cgctgttgat tgccgccagc gtgattttag gtgcggcatt tctgtctatg
    15061 ggctacttga ttagtgcaaa ggtcaaagag cgggggacgg cggccggtat ttccatcggc
    15121 gtatggttgt ttttcgtcgt catcttcgat atggcgcttt tgggtattct ggttgccgac
    15181 tcgaaacagg tcatcaccgc gcccgtcgtt gaaacggtgc tgctgttcaa tcccaccgac
    15241 atttaccgcc tgctcaacct gaccggttac gaaaatacgg ctatgtatgc gggtatggcg
    15301 ggtttgagcg gacaaatcgg cctgaccgtc cccgttttgc tgaccgcgca ggttttatgg
    15361 gttatcattc cgcttgtttt ggcagccgga atttttagaa agcgacgaat atgaagaaaa
    15421 ccctgttggc aattgttgcc gttttcgcct taagtgcctg ccggcaggcg gaagaggcac
    15481 cgccgccttt accccggcag attagcgacc gttcggtcgg acactattgc agtatgaacc
    15541 tgaccgaaca caacggcccc aaagcccaga tttttttgaa cggcaaaccc gatcagcccg
    15601 tttggttctc caccgtcaag cagatgttcg gctataccaa gctgcccgaa gagcccaaag
    15661 gcatccgcgt gatttacgtt accgatatgg gcaatgttac cgattggacg aatcctaatg
    15721 ccgacacgga gtggatagat gcgaaaaaag ccttttacgt catcgacagc ggctttatcg
    15781 gcggtatggg cgcggaagac gcgctgccgt tcggcaacaa ggagcaggct gaaaaatttg
    15841 caaaggataa aggcggcaag gtcgtcggtt ttgacgatat gcccgatgct tacattttca
    15901 agtaaatcca aacagaaatg ccgtctgaac gttgaagcca aggttcagac ggcatttttt
    15961 gtcataggtt caaacggggc aggttcgaac aactgcccca ttccttattc ctgaccgggc
    16021 gtaacggcgg aatgaggttt acatcccgcc gtaattcggt ccgcttgcgc cttcggggca
    16081 aatccaagtg atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt
    16141 tgccgcgttg atttgcaggc gcggactgcc gttttcttcg atgatttcat atacgccggc
    16201 cggacaatag cgcgtttcgg gagaggcgta ttctttatag ttcacgtcta tcatcgtttg
    16261 cggattgttc agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa
    16321 gacgctgctc aagcggtcaa aggtcaacac accgtcgggt ttcggataat caatcggctt
    16381 gcacacggcg gcttttttga gctgctcgtt gtctttgccg tgatgtttca aggtccacgg
    16441 ggctttgcct ctgaaaatca tctggtcgat gccggtataa agcgagccga ggtaaacgcc
    16501 ccatttgaat gacggacgga cattgcgcgc ggcataaagc tcttgataca gccagctttg
    16561 ttcaaacagt ttttgatagt ttcccgcttc tttgccgctg tcgaagcttt ccacttcttc
    16621 aaagttttcc aacaagggga acacggcttc ggcggcgagc atggcggatt tcatcgcggt
    16681 atgaatgcct ttgatgcgcg gcatattgag gaaccccgcc gcatcgccga ttaaaacgcc
    16741 ccccttgaac gagagcttcg gcaggctttg caaaccgcct tcaatcagcg aacgcgcgcc
    16801 gtaagcaatg cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg
    16861 ttggaactct tcaaacggcg acagatacgg gttttgatag tccaaaccga ccacgaagcc
    16921 gacggcgact ttgttgtcgt cgaaatggta aataaacgca ccgccgtagg ttttgctgtc
    16981 cagcggccag cctgcgctat gcaccaccaa accgggctga tgctgttcag acggcacttc
    17041 ccaaacttct ttgatgccca agccgtaagt ttgcggctgg ctgttttggt cgagttggaa
    17101 acgttcgatg atttgcttgg aaagcgaacc tcggcaacct tcggcaaaca gggtttgccg
    17161 cgcccaaagc tccatgccgg gctggaaact gtcggtcggc ccgccgtctt tgcctacacc
    17221 catattgccg gtcgcaatac ctttgaccga gccgtcttcg tgatacagca cttcggcggc
    17281 ggcaaagccc ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac
    17341 gacttcgcct aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taataggcag
    17401 attgaacgct tttttctccg tcaggaacaa caccttgtcc tgagttaccg tgcgcgtcag
    17461 cggcgcgcct ttttctttcc agtcgggaat cagctcattc agcgcaatcg gatcgataac
    17521 cgcgcccgcc agcgaatgcg cccccgcctc cgaacccttc tccgccacgc aaacgctgat
    17581 ttcgcgcccg ttctgttcgg caagctgctt gagtttgatg gcggcggaca accccgacgg
    17641 gcctgcgccg acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat
    17701 ggcggttcct gtgtatttat tattgaattg caaatccgta attatacaac gggaacatat
    17761 agttaccaaa tacaacaaag gtcgtctgaa aaccatattt tcggttttca gacgaccttt
    17821 gtcgaaattt caataagcac gccaccattt tacctgtccg accgcaaact ccgtctgacg
    17881 tttcggactg cgtgtgaaaa acgccttatc cccaccggca tccctccctt tcggcacgac
    17941 cgccaaaat
//
LOCUS       DAXDZG010000048        17800 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXDZG010000048 DAXDZG010000000
VERSION     DAXDZG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.048"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB78_001361"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB78_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC1269335.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(670..783)
                     /locus_tag="ACFB78_001362"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="ACFB78_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="ACFB78_001363"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="ACFB78_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC1269336.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGVAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="ACFB78_001364"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="ACFB78_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC1269337.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="ACFB78_001365"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="ACFB78_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC1269338.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="ACFB78_001366"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="ACFB78_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC1269339.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFB78_001367"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFB78_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC1269340.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="ACFB78_001368"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="ACFB78_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC1269341.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFB78_001369"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFB78_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC1269342.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="ACFB78_001370"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="ACFB78_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC1269343.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFB78_001371"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFB78_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC1269344.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFB78_001372"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFB78_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC1269345.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="ACFB78_001373"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="ACFB78_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC1269346.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="ACFB78_001374"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="ACFB78_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC1269347.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="ACFB78_001375"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="ACFB78_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC1269348.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFB78_001376"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFB78_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC1269349.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFB78_001377"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFB78_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC1269350.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="ACFB78_001378"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="ACFB78_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC1269351.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="ACFB78_001379"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="ACFB78_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC1269352.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFB78_001380"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFB78_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC1269353.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFB78_001381"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFB78_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC1269354.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="ACFB78_001382"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="ACFB78_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC1269355.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="ACFB78_001383"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="ACFB78_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC1269356.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQSTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGAFYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="ACFB78_001384"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="ACFB78_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC1269357.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFB78_001385"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFB78_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC1269358.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFB78_001386"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFB78_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC1269359.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFB78_001387"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFB78_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC1269360.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGTGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFB78_001388"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFB78_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC1269361.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFB78_001389"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFB78_001389"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC1269362.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFB78_001390"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFB78_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC1269363.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="ACFB78_001391"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="ACFB78_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC1269364.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="ACFB78_001392"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="ACFB78_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC1269365.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="ACFB78_001393"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="ACFB78_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC1269366.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFB78_001394"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFB78_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC1269367.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4908 a         3369 c         4392 g         5131 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtcaagc ttggtacatt tttgccaagt ttaactttgt ctgaaagaca ggccaatcgc
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa
     1141 gttggtatga cccgcgtgtt cgacgagcag ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagata ctgacggcta taccgccgtt
     1261 caagttacct ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt cggtcaactg
     1441 gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg gcacgatcaa acgtcataac
     1501 ttcggtgccc aacgtacttc ccacggtaac tcccgttctc accgcgttcc aggctctatc
     1561 ggtgtggcgc aagacccggg tcgcgtgttc cccggtaagc gcatggccgg ccaatacggc
     1621 aacaccaaag caactgttca aaaattggaa gttgtccgtg ttgatgcaga acgccaactg
     1681 ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcccagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcaccagc
     1861 tggtaaatgc ctacttggca aacgcccgct ctggtaaccg tgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgc gcccgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc cggtatggcg actatcctgt
     2101 cccaattggc gcgtgacgag cgtttgtttg tgattgaggc gttgactgcc gaaactccca
     2161 aaaccaaagt ttttgccgaa caagtaaaaa atttggctct ggagcaagtg ctgtttgtaa
     2221 ccaaacggct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtattggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta taaaaaagta atcatcacta
     2341 aagatgcggt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 aatcttggcg cctgtcgttt ctgaaaaaag caacgtattg gctgaaaaat gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg tcgagctgct
     2521 gttcggtgtt caagttgctt ctgtaactac cgttaccact aaaggcaaaa ctaagcgttt
     2581 tggtcgtatt ttgggtcgcc gcagcgatgt taaaaaagct tatgtaagct tggctgacgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcacc ttatgcaccc ttgcttgaaa agaaaaattc tactgccggc
     2821 cgtaacaata atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggcatttctg ctaaagtaga gcgtatcgaa
     2941 tacgatccta accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgtcgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gacggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagt tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtcttccag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgtgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg cgggtagtca aagtacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctttttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 actggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggt cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctacc ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggcatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atattttgac attgaataaa aaagactaaa aacagaaaac
//
LOCUS       DAXDZG010000049        16774 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXDZG010000049 DAXDZG010000000
VERSION     DAXDZG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.049"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            153..1217
                     /locus_tag="ACFB78_001395"
     CDS             153..1217
                     /locus_tag="ACFB78_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC1269368.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIRVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="ACFB78_001396"
     CDS             1358..1681
                     /locus_tag="ACFB78_001396"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC1269369.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="ACFB78_001397"
     CDS             1755..2078
                     /locus_tag="ACFB78_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC1269370.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB78_001398"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB78_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC1269371.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB78_001399"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB78_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC1269372.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB78_001400"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB78_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC1269373.1"
                     /translation="MSETQNEQTVQLLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /locus_tag="ACFB78_001401"
     CDS             3845..4453
                     /locus_tag="ACFB78_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC1269374.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="ACFB78_001402"
     CDS             4462..4815
                     /locus_tag="ACFB78_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC1269375.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFNAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6733
                     /locus_tag="ACFB78_001403"
     CDS             4805..6733
                     /locus_tag="ACFB78_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC1269376.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6809..7486
                     /gene="misR"
                     /locus_tag="ACFB78_001404"
     CDS             6809..7486
                     /gene="misR"
                     /locus_tag="ACFB78_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC1269377.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /gene="misS"
                     /locus_tag="ACFB78_001405"
     CDS             7499..8905
                     /gene="misS"
                     /locus_tag="ACFB78_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC1269378.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQTIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11446
                     /locus_tag="ACFB78_001406"
                     /pseudo
     CDS             9023..11446
                     /locus_tag="ACFB78_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB78_001407"
     CDS             11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB78_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC1269379.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB78_001408"
     CDS             11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB78_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC1269380.1"
                     /translation="MTDAQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12261..13010
                     /gene="trmD"
                     /locus_tag="ACFB78_001409"
     CDS             12261..13010
                     /gene="trmD"
                     /locus_tag="ACFB78_001409"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC1269381.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAKL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13025..13390
                     /gene="rplS"
                     /locus_tag="ACFB78_001410"
     CDS             13025..13390
                     /gene="rplS"
                     /locus_tag="ACFB78_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC1269382.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13711..14436
                     /locus_tag="ACFB78_001411"
     CDS             13711..14436
                     /locus_tag="ACFB78_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC1269383.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14471..15346
                     /locus_tag="ACFB78_001412"
     CDS             14471..15346
                     /locus_tag="ACFB78_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC1269384.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGILMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15423..16337
                     /locus_tag="ACFB78_001413"
     CDS             15423..16337
                     /locus_tag="ACFB78_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC1269385.1"
                     /translation="MKHLKLTLIAALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16557..>16774
                     /locus_tag="ACFB78_001414"
     CDS             16557..>16774
                     /locus_tag="ACFB78_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC1269386.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEI"
BASE COUNT         4365 a         5341 c         3688 g         3380 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcagag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccgcc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcattaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagggcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaactgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg cactgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caactgcttg tcgaacacct catcgagctg cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcaa cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcaggattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gccgctcccg gccatccgga
     6601 aacaaaaccc tgcaaacgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggctgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcgg aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcagacaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gccccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggtg aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattgcc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctgcac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcagc ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcagcag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgacc
    10561 agttcctgca cacactgacc gtcattcccg aataccgcca acacattttc ggcatcaccc
    10621 tcaacctcaa cggcggacaa tacagcagcg atttcatctt aaagacaccc ggaacacacg
    10681 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    10741 aaatgcagag tgccgccgca tacctggaac acaaaaaccc gacagccgcc gaatttctcc
    10801 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    10861 tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca
    10921 acacgaccgt taccattatc ccccttttac ccgaagacgc agaagccaaa cagcagttcg
    10981 tccgcagcct cggccccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    11041 ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaaccg catggacggc
    11101 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    11161 atgcgaattt ggcatcacac tggcggaaca tatgcgcggc agcgggctgg cacagaaaat
    11221 gatggaactc atcatccaaa ccgccgcaca gcaaggctac ctgagcatga gtgccgacat
    11281 tctcaaaacc aataccccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    11341 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    11401 aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc
    11461 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11521 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11581 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11641 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11701 gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg cgtttgccgc catgacagac
    11761 gctcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggctg
    11821 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11881 gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa
    11941 ctccaagtca aattcgaagg catagacgac cgcgattcag cattctcatt gcgcggttac
    12001 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    12061 gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac
    12121 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    12181 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    12241 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    12301 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    12361 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctatatcgac gaccgcccgt
    12421 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggcg atagaacacg
    12481 ccaaagcaca atcttcccaa accgcaaaag tcatctacct cagcccccaa ggaaaaccgc
    12541 tgacacacca aaaagcggca aaactggcag aacttacgca tctgattctg ctgtgcggac
    12601 gctatgaggg aatagacgaa agactgctgc aaagcagcgt cgatgaagaa atcagcatcg
    12661 gagacttcgt cgtttccggc ggagagcttc ccgccatgat gctgatggat gcggtattga
    12721 ggctcgtacc cggcatattg ggcgacattc agtctgccga acaggattcg ttctcaagcg
    12781 gtattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg gctgttccgg
    12841 aagtattgcg ctccggaaat catggcttga tagcggaatg gcggttggaa caatcgctgc
    12901 gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat
    12961 cccgcctctt gaataaaatc ctacaagagc aacgggaaat ccaatcataa tttaggaaaa
    13021 aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgcctga acaaagaaat
    13081 ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg aaggtacccg
    13141 cagccgtctg caagcctacg aaggcgtggt tatcgcccgt cgcaaccgtg gtttgaacag
    13201 caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt gaacgtactt tccaactgta
    13261 ttcccctact gttgagaaaa tcgaagtcaa acgccgtggc gacgtacgcc gtgccaaact
    13321 gtactacctg cgcggtctga ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg
    13381 caaaggttga ttcaaaccgt tttcccccaa tgccgtctga acgttcagac ggcatttttt
    13441 attgccgcga tgccggcaag gcaacgatat gcctgaccct catccacctt aattaacaat
    13501 cactcccgcc tattcaagca gaatttacag actaaacgtt atacagtatc atatccgcca
    13561 tataacgata accgctttgc caacttccct ttcaaaactg caaatcatta tgacaccttc
    13621 cctcttactg tcaggattga ccttccgact cttcctcgcc ctgattgccg tattcctttt
    13681 atggggtgtt tacctttggg cggtatcggt atgagcatca tcgttgaaaa cctgacggtc
    13741 agctaccgcc gccgccccgc cgtacaccac gtcgacatta cttttgaaga acatagtatg
    13801 tgggcggttt tcggtcccaa cggcgcagga aaatctacct ttctcaaatc cttaatggga
    13861 ttgcagccta tcgatacggg cagcatccgg ctggacggat tgacccgtca aaacattgcc
    13921 tatctccccc aacaatccga catcgaccgt tcccagccta tgaccgtttt cgacttggcg
    13981 gcaatggggc tgtggtatga aatcggtttt ttcaaaggga taaataccgc tcaaaaacaa
    14041 cgcgttcacg aagccttgga gcgtgtcggt atgcggcagt ttgcccgccg ccaaatcgcc
    14101 cacctctcaa acggacaatt ccaacgcgtc cttttcgccc ggatgctggt tcaaaatgca
    14161 aaattcctgc ttttggacga acctttcaat gctgttgatg cacggacaac ctacgagctt
    14221 ctcgacgtgt tgcaaaaatg ccattgcggc ggacacgcta tcattgccgt tttgcacgat
    14281 tacgaacaag tccgtgccta ctttcccaat accctgctgc tcgcccgcga aaaaattgcg
    14341 gcaggcgcaa ccgagaccat tctgacagaa tcctacctcg cccaagccag cgccaaaatg
    14401 cagcaacagg aaagccccga ctggtgcgcc tcataaatac tgcccgaaac cacgccccac
    14461 accagacacc atgaacctct acgacctgct ccttgccccc tttgcagaat tcgacttcat
    14521 gcactacgcc ctcgcatccg tcttctgcct gtccctcagc gccgcacccg tcggcgtatt
    14581 cctcgtcatg cgccgtatga gcctgatagg cgacgcattg agccacgccg tcctgcccgg
    14641 tgccgccgtc ggctacatgt ttgccggctt gagcctgccc gctatgggtg tgggcgggtt
    14701 tgccgccggt atgctgatgg cgctgcttgc cggactcgtc agccgcttta ccaccctgaa
    14761 agaagatgcc aactttgccg ccttttacct gagcagcctc gccatcggcg taatcctcat
    14821 cagcaaaaac ggcagcagcg tcgatttact ccacctcctt ttcggatctg tgcttgccgt
    14881 cgatattccc gcactgcaac tcatcgccgc cgtctccggc ctcacgctca ttacccttgc
    14941 cgtcatctac cgccccctgg tgctagaaag catagacccc ctgttcctca agtccgtcaa
    15001 cggcaaaggc gggctttggc acgtcgtttt cctcatcctc gtcgttatga acctcgtatc
    15061 cggcttccaa gctctcggca tcctgatgtc ggtcggactt atgatgctgc ccgccattac
    15121 cgcccgctta tgggcaagaa atatggggac gctcattctg ttgtccgtcc tcatcgccct
    15181 tttttgcggt ttgatcgggc tgctcatttc ctaccacatc gaaatccctt ccggccccgc
    15241 catcatcctc tgttgcagcg tcctttatct cttttccgtc atactcggca aagaaggcgg
    15301 catcttgccc aaatggttca aaaaccaccg ccaccacacc acctgacccc gcaagccgct
    15361 tgaacaaaca ggcggcttgt tttaccataa tgaatgttat gttatatcac aggaaaacag
    15421 ttatgaaaca cctcaaactt acccttattg ccgcattgct ggccaccgcc gcaactgccg
    15481 caccccttcc ggttgtaacc agtttcagca ttttaggcga cgtagccaaa caaatcggcg
    15541 gtgagcgcgt agccgtacaa agcctcgtcg gagccaacca agatactcat gcctatcaca
    15601 tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact cgtcctgctc aacggcttgg
    15661 gacttgaagc cgccgacatc caacgcgccg tcaaacagag caaagtatcc tatgccgaag
    15721 cgaccaaagg catccaaccc ctcaaagccg aagaagaagg cggacaccat cacgaccacg
    15781 atcatgacca cgaaggacac caccacgacc acggcgaata tgacccccac gtctggaacg
    15841 accctgttct tatgtccgac tatgcccaaa acgtcgctga agccctgata aaggccgatc
    15901 ccgaaggcaa agtttattat caacaacgct tgggcaacta ccaaatgcag cttaaaaaac
    15961 tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc cgccaaacgc aaagtcctga
    16021 ccgggcacga cgcattttcc tacatgggca accgctacaa catcagcttc atcgccccgc
    16081 aaggcgtgag cagcgaagcc gagccgtccg ccaaacaagt cgccgccatc atccggcaaa
    16141 tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat caaagacacc cgcatggttg
    16201 accgcatcgc caaagaaacc ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg
    16261 gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca caacgtcaaa gccttgacca
    16321 acgcgatgaa gcaataaaac cgtttgattg gtttgaattt gaaaaggcaa aaggccgtct
    16381 gaaaattaaa tacgaggttt tcagacggcc ttctcttgtg caataaagca gtaaacaaat
    16441 attgataaga aaaaaacaat cagtattgaa aatatcgtta aaaatattat ttagacagat
    16501 tacatggaat ttcattaaca tcagtagact ttgatttcaa tcaagaaaac aatgtcatga
    16561 aaaatattct cttagtattt gttagctttg tgccattatg tgtccgcact gatctgccgc
    16621 tgaatatcga agacataatg accgacaagg gaaaatggaa actggaaact tcccttacct
    16681 acctgaacag cgaaaacagc cgcgccgcac ttgcctcacc ggtttacatt cagaccggct
    16741 ccgcttcctt tatccccgtc ccgaccgaaa ttca
//
LOCUS       DAXDZG010000050        16678 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXDZG010000050 DAXDZG010000000
VERSION     DAXDZG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.050"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            491..2026
                     /locus_tag="ACFB78_001415"
     CDS             491..2026
                     /locus_tag="ACFB78_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1269387.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            2023..2115
                     /locus_tag="ACFB78_001416"
     CDS             2023..2115
                     /locus_tag="ACFB78_001416"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC1269388.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(2167..2589)
                     /locus_tag="ACFB78_001417"
     CDS             complement(2167..2589)
                     /locus_tag="ACFB78_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC1269389.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRDGHIVRAQTLDASGKVEREWQP"
     gene            complement(2783..4003)
                     /gene="lysA"
                     /locus_tag="ACFB78_001418"
     CDS             complement(2783..4003)
                     /gene="lysA"
                     /locus_tag="ACFB78_001418"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC1269390.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASFGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(4014..4184)
                     /locus_tag="ACFB78_001419"
     CDS             complement(4014..4184)
                     /locus_tag="ACFB78_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC1269391.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            4255..4578
                     /gene="cyaY"
                     /locus_tag="ACFB78_001420"
     CDS             4255..4578
                     /gene="cyaY"
                     /locus_tag="ACFB78_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC1269392.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            4611..5030
                     /locus_tag="ACFB78_001421"
     CDS             4611..5030
                     /locus_tag="ACFB78_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC1269393.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            5052..5558
                     /gene="luxS"
                     /locus_tag="ACFB78_001422"
     CDS             5052..5558
                     /gene="luxS"
                     /locus_tag="ACFB78_001422"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC1269394.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            5702..8494
                     /gene="polA"
                     /locus_tag="ACFB78_001423"
     CDS             5702..8494
                     /gene="polA"
                     /locus_tag="ACFB78_001423"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC1269395.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            9056..9751
                     /locus_tag="ACFB78_001424"
     CDS             9056..9751
                     /locus_tag="ACFB78_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC1269396.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INDKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(9743..9853)
                     /locus_tag="ACFB78_001425"
                     /pseudo
     CDS             complement(9743..9853)
                     /locus_tag="ACFB78_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9991..14397)
                     /gene="app"
                     /locus_tag="ACFB78_001426"
     CDS             complement(9991..14397)
                     /gene="app"
                     /locus_tag="ACFB78_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC1269397.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTTFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            14835..16181
                     /gene="mnmE"
                     /locus_tag="ACFB78_001427"
     CDS             14835..16181
                     /gene="mnmE"
                     /locus_tag="ACFB78_001427"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC1269398.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            complement(16477..>16678)
                     /locus_tag="ACFB78_001428"
     CDS             complement(16477..>16678)
                     /locus_tag="ACFB78_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269399.1"
                     /translation="GAVSVFSDKFLKLKISSFPQGQKTKNRNLKFVIPAKAGIQFVEF
                     QLFRINFETLIPSFPRKRESGT"
BASE COUNT         3686 a         4239 c         4585 g         4168 t
ORIGIN      
        1 ggccgccttg tcctgatttt tgttaatccg ctatatttcc cgccatccca aaaacgaaga
       61 gcggcaggaa tttatcggaa aaacagcaac ctttccgccg tcattcccgc gaaagcggga
      121 atctaggttc gtccggtttc ggttatttcc gatagattcc tgccgcgttg ggggtctgga
      181 ttcccgcctg cgcgggaatg acgggacttt aggtttctgt ttttgttttt ctgtttttgc
      241 gggaatgacg gggaagttgg cggttgtttc ttatggagtg gtttttgtat ttggataaaa
      301 aatttttcgt tttcaagcct tcaccgcttg ccatcggcgt taaaattttt tacgataagc
      361 acatagattg taaacaatcg gccacaagcc ggtttgtttt ttcagaagac attatccctg
      421 tcagacgctg tttctatata tgtttgccta taacggcttg tttttaataa ataattcaag
      481 aggtatcaac gtgtctgatt ccaaaacgaa agaacgcgcc acattcggca cgcgccgcgc
      541 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      601 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      661 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc ggggttctgc
      721 gcccttggct ttccgccgcc tcggacgctg gttcgagccg gtcggctggt ggaacgtgat
      781 gaccaatatc gtcatctgca tctattacgc agtgattatc ggttgggcgg caagctatac
      841 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      901 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggccc
      961 tttggcgggc gtgtgggttt ttaccgccgc cattatggct ttgggcgtgc aaaagggcgt
     1021 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
     1081 catttcgctg acgctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
     1141 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
     1201 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
     1261 gggcggaacg gggctggtgg tcggttttgc caacagcagc ttcgaactgc tcgcgggcat
     1321 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggcaaggcgg tcaacgaggt
     1381 tgcctccggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcgcc
     1441 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1501 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1561 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1621 tacggcgacg gggctgcctg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1681 tgttgccgcc ggctttgttt atgttgccgc catcatcatc ggcggcaggt tgccggaatt
     1741 acgcaggcac ctgaacgcct tgtcctccat ccgcgtcggc ggcttgtgga cggcctgcgt
     1801 cgtgtttacc gtcgtgatgc tcggctatat gctgtatcag gacaccgccg gattgttgga
     1861 aaaaaactac ggcgattatc cggatggttt cctcaatatt ttcggctggg ggatgtcggc
     1921 ggcgttaatc atgttcgggc tgctgctgtc gctgctgcct tggaaacacg gtcaggattt
     1981 taatgtcaaa gacgaacacg aacatgaaca aggaggagaa aaatgagtac ttccgccatt
     2041 gtgatgatgg ttgccgccat cgcggtgatt tggggcgggc tgctgctttc cctgttaagg
     2101 ctgccggaag agtaagcctt tagaacgtta aaaatgccgt ctgaaccgct tcagacggca
     2161 tttgttttac ggctgccatt cgcgttccac cttgcccgac gcatccaaag tttgcgctcg
     2221 gacgatatgc ccgtcccggt acaggatgcg cgtccgcaat atgccctgtt cggtatagcc
     2281 ttgcgccacg ccgttttcaa ccggcgtgtg cgaatgcagt ttgccgtttg gatggtaaat
     2341 atcgacaaag cggtcgaaca cgccgttttt gacgatgctt ttcaagacgc gcccgtttga
     2401 ggcgcgggtg ctgaatgcgc cgtccggcgt gcctgaaggc ggccggctgt atgggttggc
     2461 gggttggatg ggcgcgcttt tcggcagggt gcgaagcggc ggccggtcag agggaagggc
     2521 ggtgcagccc gccgaaagta gggcggcgga cgaagcggta agcaggagga taatcggagc
     2581 gtgcttcatt tcaattcctt agatattgca ccccataaaa ggggcgcgta atttttttca
     2641 acaattccct tcgtcagtcg ggcgagacgg ggatttgttg cgtcgtcatt tgaaaacagg
     2701 ctgtgtttga atattccgcc aagatgtcac tttaaacgga caatgccgtc tgaaggcttc
     2761 agacggcatt ttgatgttcg gcttataggc aggcgagttc gtttgccatt tgctgttcca
     2821 aggtttcgcg ccggcggatg agtttgtatc cgccgccgtc aaccaacacc tccgccgcac
     2881 ggttgcgcgt gttgtaattg ctagccatac tggccccgta tgcgcccgcg ctgcggataa
     2941 gcagcaaatc gccttcttcg caggcgatgg tgcggtcttt gccgaggaag tcgccggttt
     3001 cacaaatcgg gccgacgatg ttggcggtca gaggctcaat gtttttggtt tcaaccgctt
     3061 cgatgtggtg gtaggcatcg tatagggctg ggcgcatcaa atcgttcatc gccgcatcga
     3121 ccatcacaaa gtttttctct tcaccgtgtt tgacaaattc gacgcgcgtc agcaatgcac
     3181 ctgcgttgcc gaccaagctg cggcctggct caagaatgag tttcagacgg cgtgtcccca
     3241 tcagtttttg aaccgctcgg gcatacgcac ccaaatcggg gacgccttcg tctttgtaaa
     3301 caatgccgac gccgccgcct aagtccaaat gttccaaaac aatgccttcg gcggcaagag
     3361 cgtcaaccaa aatcaaaatg cgttcgcagg cttcgaccag tgggcttaag tcggtcagtt
     3421 gcgaaccgat gtggcagtcg atgccgatga ttttcaaatt gggctgttgt gcggcatggc
     3481 ggtaggcttc gagcgcgtcg gcgtaggcga tgccgaattt gttggctttc agacctgtgg
     3541 agatgtaggg atgggttttt gcatcgacat cgggattgac gcgcagggag acgggcgcgg
     3601 ttttgcccaa acgcgcggca attttctgaa tgcggtcgat ttcggggatg ctttccatat
     3661 tgaagcattt tacgccggca ttcagcgcga actcgatttc cgcctcgctt ttgcctacgc
     3721 cggaaaaaat cgttttcgcc gcatcgccgc ctgccgccaa aacgcgtgcc aattcgccgc
     3781 ccgacacaat gtcaaaaccg ctgccaaaag aagcaaagtg tttgataatg ctcaggttgc
     3841 cgtttgcctt gacggcgtag cagacaagcg ggttcaaagc ggcaaaggcg gtttgatagt
     3901 tttcaaatgc tccggtcagc gcggattggc tgtacacata aagcggcgtg ccgaattctt
     3961 cggcaaggcg ggggtagggg acttgttcgc aaaacagggt catattttcg ttttcatttt
     4021 tgggttggtg gggcggattg cggtttgcct tgaagctgca aaccggtttg gatcacgccg
     4081 aaacgcgccg tatcgccttc tttgggcagg tagaggccgc ctttgtaacc gcaggcggaa
     4141 agcaggaggg cggttgccgc cgcaaaaaat acgccgtatt tcatcggtaa acttccttca
     4201 taagcgcgaa tgtggcaaga ttcggcatct taaacaaaaa acatgcaaaa agctatgatg
     4261 accgaaagcg agtttatccg cgcgagcgaa gcattatttg aacacatcga agaccaaatc
     4321 gacgaaaacg gctgggattt cgactgccgg tttgccggaa acgtcctgac catcgaagcc
     4381 ggagacggca cgcaaatcat cgtcaaccgc cacacgccca accaagaatt gtggattgcc
     4441 gcaaaaagcg gcggctacca tttcgccgaa caaaacggca aatggctggc aacgcgcgac
     4501 agccgcgatt tttacgacgt tttaaacgaa gccctgagcg cggcttcggg cgaagcggtt
     4561 gagattgccg aattgtgatt caattttcga tataaaaaga ttgttaccca atggcacaat
     4621 tacctctata tcggactgcc gaaatcggaa attttactgt cggcacgcct aaagttttag
     4681 aatcattttc caaacatatc ccttatggtg ttgtctttga agatgatggc aacacgggct
     4741 acttctatgc cgcttcgcaa gaggggattt tggatgcctt gcacatctac aatgtcgaag
     4801 atgtatccga caaacatatc cccaatcatg tcttgatttt atgggatggc gcctgcacca
     4861 tagccgcatt gtgtatcaac gactacatcc atgccgtcta tgattttgtc gaacaggcag
     4921 gatattgccg caacggcttc cctgaagcag gcggcgaatg ggtaaaagtc gaaaaccgcg
     4981 tcttggacga tgaattgctg gacaaaatcc tatcccgaaa acctacataa cccgcacaaa
     5041 aggataccca aatgcccctg ttagacagtt tcaaagtcga tcacacccgt atgcacgccc
     5101 ctgccgtacg cgtggcgaaa accatgacca cgcccaaagg cgacaccatt actgtattcg
     5161 acctgcgctt ttgcatcccc aataaagaaa tcctgcccga aaaaggcatc cacacgctgg
     5221 agcatttgtt tgccggcttt atgcgcgacc acttgaacgg cgccggcgtg gaaatcatcg
     5281 acatttcccc gatgggctgc cgcaccggtt tttatatgag ccttatcggc acgccttccg
     5341 aacagcaggt tgccgatgcg tggttggctt cgatgcagga tgttttgaat gtcaaagacc
     5401 aaagtaaaat ccccgagttg aacgaatacc aatgcggcac ttatctgatg cactcgctcg
     5461 ccgaagcgca acaaatcgcg caaaacgtgt tggcgcgcaa agtggcggtt aaccgaaacg
     5521 gcgatttggc tttggatgaa agcctgctga acgcttaatc cgccgcaaat gctgtctgaa
     5581 caagggtttc agacggcatt tgccttttcc gttataatcc ggggttgtac gggggcgggt
     5641 tttaagccgg catcgtcctt ccctattttt ctgtccctta tcggtttcaa gcgggttttt
     5701 tatgtccaac agacctacac tcctcctcgt tgacggctct tcctatctct accgcgcgta
     5761 tcacgcgatg gggcaaaacc tgactgcgcc tgacggtgcg ccgacgggtg cgctgtatgg
     5821 cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt gcgcggtggt
     5881 ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca aggcgacgcg
     5941 cccgccgatg ccggacgatt tgcgcccgca ggcggaagcc ctgccggatt tggtgcgcct
     6001 gacgggctgg ccggtattgg tgattgggca agtggaggcg gacgatgtga tcggcacgct
     6061 ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgacgggcg ataaggatat
     6121 ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcggag aaacgctgga
     6181 tattgaaggc gtgaaggcaa aattcggcgt tcgccccgac caaatccgcg attatctcgc
     6241 gctgatcggc gacaaggtgg acaacgtacc gggcgtggaa aaatgcggcc cgaaaacggc
     6301 ggtgaaatgg ctggaagcct acggttcgtt gcaaggcgtg atggaacacg ctccggaaat
     6361 caaaggcaag gtcggcgaaa acctgcaagc cgcgctgccc caactgccgc tgtcgtatga
     6421 tttggtcacg attaaaaccg atgtggactt gcacaccgag ctttcagacg gcatcgaaag
     6481 cctgcgccgt accgcgccga aatgggcgca gcttgcggtt gatttcaaac gctggggctt
     6541 ccgtacttgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg atgatttgtt
     6601 cggtagcgac agcatcggcg agcaggcggc tttgaatgcg gaaataccgt ctgaaaaacg
     6661 agctgaaaaa gccaccgccc ctgaaaaact ggattatcaa gccgttacca ccgaagctca
     6721 gtttgccgcc ttgttggaca aactagcgaa ggcggacaca atcggcattg atacggaaac
     6781 cacgtcatta gatgcgatga atgccgagct ggtcggcatc agcattgcgt tccaagcagg
     6841 cgaagcggtt tacatccccg taggccacag cctgactgcc gcgcccgaac agcttgattt
     6901 gcaagacgta ttaggccgtc tgaagccgca tttggaaaac cccgccctga aaaaaatcgg
     6961 tcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgcgt taaacaacat
     7021 tgccggcgac gcgatgctcg cttcctacat catcgagagc catctcggac acggcttgga
     7081 cgaattgtcc ggacgctggc tcggtttaga aaccattacc tacgaatcgc tgtgcggcaa
     7141 aggcgcgaag caaatcagtt ttgccgatgt cgccatcggg caggcgaccg aatacgccgc
     7201 ccaagatgcc gatttcgccc tgcgcctcga agcgcgcctg cgcgcgcaaa tggacgacaa
     7261 acaacttgaa atgtatgaaa aaatggagct tcccgtcgcg caggtattgt ttgaaatgga
     7321 atgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg aactcggcgc
     7381 ggagctgatg aagctcgaac aggaagccta cgccgctgca ggtcaaccgt ttaacctcaa
     7441 ctcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga caaaagggct
     7501 gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac agctcgcgcc
     7561 cgactacccc ctgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac tcaaatccac
     7621 ctacaccgac aaactgcccg aaatgatttc gccccgggat aaccgcgtgc ataccaccta
     7681 tgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc tgcaaaacat
     7741 ccccattcgc accgccgaag gccgccgcgt gcgccgcgcc ttcactgcac cgccgggcag
     7801 cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc acctctccgg
     7861 tgacaaaacc ctcattgccg catttcaaag cggcgaagac gtacaccgcc gcaccgccgc
     7921 cgaagtgttc ggcactgcgc ccgaaaacgt ctcgcccgag caacgccgct acgccaaaac
     7981 catcaacttc ggcttaatct acggtatggg gcaatacggt ttggcaaaat cattgggcat
     8041 cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgttatc ccggcgtcgc
     8101 cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg aaaccctgtt
     8161 cggcagaagg ctctatttgc ccgacatccg caacaaaaac gccaacgccc gcgcaggagc
     8221 cgagcgtgcc gccatcaacg cccccatgca aggcaccgct tccgacctca tcaaacgcgc
     8281 catgataaac gtgcgaaact ggctttcaga cggcatcggc agcaaactgg tcatgcaggt
     8341 gcatgacgaa ctggtgctgg aagtcgttga aaccgaactg gattttgtca aagaaaaact
     8401 gccgcagatt atggcgaaag tggacggcgg attattggat gtaccgctgg tggctgaggt
     8461 tggcgtaggg gagaattggg aagaggcaca ttgagaatgc acaagtattg aactgttacg
     8521 ttttgattta tcaaaattta aggaagtaaa atacccgatt cgaatgattg gaaatcaata
     8581 gcaagccgta ggtcggatac ttgtatccga caaaagcctg ccatctcaaa tagcagtcgg
     8641 attcgagaat ccgacctgcc aaaccgggcg cggacgctcc ggtcggcagt tagcgcacaa
     8701 atcgaacaga acatcacaaa aaagcctgat tcggattttc caatcgggct tttttgcgcc
     8761 cgttttgtca tcccgtgaaa tatccgcata acaaaaataa tgcaaaatca tgacaaaacc
     8821 ggcgcgaggt tacacaaacg gatgaaatca accgatattc aaacacagcc atttttagcg
     8881 cattttcagc gtatctttat gcggaaaatt tcgtgaacag gttttttgca caggcttccg
     8941 ccgcgtttcg cgggatggga aaccgtatta gaaaacggac gcacgcaaca taaaccccga
     9001 aagtgatgat aagatgatga tttaacgtac tgctttaatt atttaaggaa ttatcgtgtt
     9061 tcccgacaaa tacaagttga gtttggaaga aaatattttt ctggcaaaga aagtattggt
     9121 tgctcaaatt cacaacctca gccgttttga gaattgtcag acgaccttgt tgcagaccga
     9181 gcaaattatc aatgacaaaa atgtagcctc cgcgtcactg gaagacatcc aaaccatctt
     9241 gaacctgaaa cgtgcctatc aatatgtgat ttcgcatatt tcaaacggcg aaccggtcaa
     9301 tatttcactc cttaaaagaa tcaacaacat tgttgccaag gacgattctt tggtacccgt
     9361 ggatttccgt accggttcgg tcggcgtaac gctattggac ggttcccgtc atgccccgaa
     9421 tccggtgaag gaaattgaag tggcccgcgt gttgcaaaat atcggactgc aaagcggttc
     9481 gacgacggaa gcagccgttc gtttcatgct ctattgtatg cggcagcagg ttttttggga
     9541 cggcaacaaa cggacggcaa ccctatttgc caacggcctg atgatggcgg ggggctgcgg
     9601 tatcctggaa atctccgaaa tgcagatgcc gcgattcaat gaaaaactat ccgcattcta
     9661 tcgcaccggc gacgataccg atatttccaa gtttgtgtat caaaattgta tatcgggcat
     9721 agactatttc ggcgcggacg aagatatata gtggattaaa tttaaaccgg tacggcgttg
     9781 cctcgccttg ccgtactatt tttactgtct gcggcttcgc cgccttgtcc tgatttaaat
     9841 ttaatccact atagatttcc ccgactcgcc gaacatgggc taaaaatcaa tttgacggtt
     9901 atcagacgat ggagtaggca caaggtggca gaaaaagggg tttgacagtg cacggcggca
     9961 tcgtcaaacc cctttcggca tatccggcgg ttaccagcgg tagcctaatt tgatgcccgc
    10021 gctgtgctgc gcttccaatt gcggcccctt ggcggcggca gcgtggaggg acagcgtgaa
    10081 acctttgatt tcggcgttta cgccccattc cgcactgcgg gttttgccga aatcctgcgc
    10141 caatacggcg gtattgacgc gcgttcggac tttgccggaa gcggcatcgg tataggacag
    10201 gctcaaataa ggcgtgatgg aaatgtgttg cgccggtttg aatgaataat ctgccttaat
    10261 gcccgcgcgg tagcggttga atgcaaggcc cggggtggcg atattgacgt tttcgtatcg
    10321 gtaatccgct ttttggacga aatagcgcgt tgcgccgatg tgcggttcga tgccgaatcc
    10381 gccgaaacct gcgcggtatc ttgcctgaat gccgtaatgc agcacgcggc ggcggatttt
    10441 gcctctgatg ccgtctgaaa ggctgccgct actaaaaccc gcgcccgcgc tgatgccgat
    10501 gtcgaacctg ccgatgccgt attgcccgaa aacggcaccg tgggcaagcc gtgccgagtt
    10561 gccgatgccg tcgtcgaagg tgttttcggt ccggttgtgc gaaaacagga tgccgacgcg
    10621 cccgctgccg aggtttttct gcataccgat ttggcgcagg tcggtttgtt ggcggtaggc
    10681 gcggaaatct tgcgaacggt agtgtttggt gtcccggatg ccgcttgtcc aaacggcgtt
    10741 gcggcggtct tcggcaaaca cgcggtccaa ttcgtcctgt acggcgaaaa cgctgttgag
    10801 cgtggcggaa aattcactca aaccgctatt ggcataacgg ctgatcaggt cgcgctgcgg
    10861 ttggggttgc ggttggggct gcggttgcgg caaatcccgg cgggcgcggc gggcgcgggg
    10921 gaaggtggtg gtagccggcc gggtttccgc ttcgcgctgt ttcgccaagg cggtgtcttt
    10981 atccgcctgc acccgttttt tctcttcctc cgcctgcata atgccggcat tttccccgcc
    11041 tgcctgccgg gccggttcgg caacgctttc tgccttttcg gtggcatcgc gcccggccgc
    11101 aatcagcgcg tcaaggcttt gcgcgttgtc tttttccgcc tgttgtttgg cggcaagttg
    11161 tgcctgtttt gccgtcaagg cggcctctgt ttctcccgcc ttgccgagtt tgtcggaaag
    11221 ctcttgttct ttgaccggat tatgcaggcg gaactcgccg tctttgcgga taagctgata
    11281 acgccatgcg ccggcatcga cgtgttcgtt ttgcagggtg aaattaagat tttcggacag
    11341 cggtgtgttg tcttttcctt ccactaccgt caattgctcg agacttacgg gttcgttgcc
    11401 ggtattgttg acagccaagg tgtaagtgcc ttcggaactt tccgccagct tcaatttgcc
    11461 gctgcggtag ccgaagagtt ccgacataaa gcggaatgtt ccctgaccgt tcaatttgcc
    11521 gtttaccgtc agcgtgttga aacgggattc tgccgaagtt ggcggcgtaa cggataatag
    11581 ggaacggcgc gaacggcggc gcggcgcatc tgccgcactg ccggtttgcg cgcctgccgc
    11641 atcgtgtcga taggcggaat tgagtgtaat ggtggcgttg tcaaggttta aattgcctaa
    11701 ttccgtgccc gacggcagcg tccattcgct gtcttttaag tgtaatgccg tatccttgcc
    11761 gccgctgatt tttccggtaa agcggctgtt ttcaaaatgg aatactgcct tatcggctag
    11821 ggagacattg ccgttgagtg cggaatggct tacgtttgcc ttagcgttgt cggaaagcgt
    11881 cagactgccg ttttgtacgg cgttgttgct tagattaaat gaagcattgt ccgaagccga
    11941 tgtgttgccg tttaatgtgg cttgattaaa tgttgcttgg gcattgccca cgaggctaag
    12001 gttgccgttt tgggtggcgt tgcgcgtaac cgtatagtgc gtgtctccgc ctgcactaag
    12061 attgccgttg agtgtggcaa gtcctgtgag atttaaatga gcgtgatcgg caaggctgac
    12121 attgcctctg atgtcggtct tgctcaatga agcaatcact ttatcgtcgg taatggtttt
    12181 ttcggtacaa cttgtcagac ccgtccagtc cgaacgtgta cagattgtgt ggctttgatg
    12241 cggtgcgaca ccgaaaactg cttgggcgtg attgcttaaa tgccaatcgc cttccacttt
    12301 ggcaacattg cgggaaacca ccgcttgtcc gccctgaata tggaagtttt ccgctttaaa
    12361 tgtgcggtcg atccaatcgt tgtcccacac gatttctcct tgtgggatac cttccatttt
    12421 tgaccacccg cttcctaaat gattgtaggc gtgcggtgtc ggtctgccgc tgaaaaacag
    12481 tttgccgttt gtttgcgtga tattgccgtt taaatttgtt ccgccggaaa gcagtaaagt
    12541 gcgatccgct tcttccggtt ggtaattcag attgagccgc ccgttcgttt tggttgcatc
    12601 tttctcgcca aaccaaccgt tgtaggcaat ttcttttttg ctatccaagt tgttgttatt
    12661 gccggttgta gtaatatctt tattgcctgt aatggtaacg gtggattctt tgtcttgatt
    12721 gtggttgaca atcatcgccc cttcatcggt attttgaatg cggtggaacg aaagcgaatg
    12781 cccgttcaaa tccaaacgtc cgccgcgaaa gccgaaatag agtttgtcgg ggttgaactg
    12841 attatcggca ttcagttgca ccgtccccct gccgctgacc aagccgattt cactaaaggc
    12901 ttgttttttg ccttgatcgt ccgcctgctg atctaagatg actttaccgt cgcccacgct
    12961 gaccgagcct tggttttccc ctttggcttg aaccagcagc gtgcctttgc cgattttgga
    13021 caggcggtcg tttgccacgc cgtttacttt ccaagtaacg gtactgccat cactgatatg
    13081 aacgcccgcg ccttgccacg tttcgttgtt tttaggcgag accgtaaaat taccctcaaa
    13141 atacaaaccg cccgcgcctt ggttgatgtt gctggtaagt atcaattcac cttttccttt
    13201 gtcaataaag gaaatatttt ctccattatt cagtctgggt cgataactgt tgaccccacc
    13261 tgcagcatga taaacaggtt ctcttgctgt ctcggataaa gaaacattaa acaattgaac
    13321 ggttcgtgtt tttaatctat aaggtagaga atagtgttta tgtttggcat cgatttttcc
    13381 tgcgccatta ttattgtcgt taaaaaagta tttcccattt tgatgtggtt cgtagaatac
    13441 tgaatgggta tctccagcaa agatttcatc atagaaccaa tctttacgaa ctagctggaa
    13501 gccattgctt tttcctatat aggggttgcc tgtttgcaat accccattaa ttaaccactt
    13561 ttgcttttgg gcatcataga taaacattgg tgagccactg tcgccaaatg agcctcctgt
    13621 tggtaaaaaa ccatatgggc tatgtttaat tttttcgcta cctaagttga ctgtgccacc
    13681 acctgatcca ttttgtgcaa aggtattgcc accgacgagc caagaatatg cgcttgcaat
    13741 atgatatgaa ctttcgcggt tattgggttc gtcttcatca gaccgccaat attgtctgcc
    13801 tgctccgatt cgaacacgat cagggtattt atttaaatca gcgtatttcc acccatccat
    13861 ataactggtc atctcaacag gttctgcatc tgtgacaaat ttgtgcaaac gcggcatatg
    13921 ataatcgccg ccataaggat ggccgttagt ccctgcttta taattatttc ttttcacaat
    13981 ttggtaagaa aagcggtgct gatcgggatt gcttccctcc gcaccaaaat caacattgtt
    14041 atagccgccg ttatgtgcca cgctcacaat atattgatcg cccgccaatg ccgccacgcc
    14101 gttacgcgat accacagaaa aatcaatcat cggggctttc gtcatcgatt tgccgaccaa
    14161 ctcccctttt ttgttgtaaa cctcaatatc tttcgccccg actgcaaact tgcctttatt
    14221 ttcggcaaag tcgcgatagt attggtagtt gatgccgaaa taagtgtgtc ccgcccgggc
    14281 ttggggcaga atgccgaacg acaggcatat ggctaagtaa gcgggcgaga agcggatgcg
    14341 gccggtttta ggggctttgc ggtgtgtttc ggttgtccgt ttgtcggttg ttttcattat
    14401 ttttccttat ctgacgggat tcggtttgtt tgggagggcg cggcttccgc ttccgggcgg
    14461 cgcgcgggat gtggctatat gtgcggttcg gcgtattttg ccgcttctgt tcggagatgg
    14521 tgcagggaag ggcgggaagg aaaaaaaggg gatttttatc aactcgccgg agcaatcgat
    14581 gggttgcaca taaaggcatc attttgaata cgaaatcata tcacacttta ctttatcccc
    14641 gttccttttc ggtcggaacc ccgccgtttt cggcggtaaa aaccgaccgg aggcggaatg
    14701 cggggcaggg ttttgtgccg tgcggtgtcg gaatatcgtt ttacacaagc cggcaatgcc
    14761 atctgaaagc cgaaccgtcg gacttcagac ggcatttgct ataatcgcgg ctgttttgaa
    14821 ttttcggggg ttttatgtcg gataacgttc caacgattgc ggcagtcgcg accgcgcccg
    14881 gacgcggcgg cgtgggcgtg atacgcatat cggggaaaaa cctgttgccg atggcgcagg
    14941 ctttgtgtgg gaaaacgccc gagccgcgcg tggcaaccta tgctgatttt acggacgcgg
    15001 acggacaggc aatcgatagc gggctgctac tgttttttgc cgcgccggca agttttacgg
    15061 gcgaagatgt catcgagctt cagggacacg gcgggccggt agtgatggag atgctgctga
    15121 accgctgttt ggaactgggc gcgcgccttg ccgagccggg cgagtttacc aaacgtgcgt
    15181 ttttaaacga caaactggac ttggcacagg cggaaggcgt ggcggatttg attgacgcat
    15241 ccggccgttc ggcggcgcgt ctggcgttgc gctcgctcaa gggcgatttt tcgcggcgga
    15301 tacacggttt ggtcgaaggc ttgattacct tgcggatgct ggtcgaagcg gcgctggatt
    15361 ttcccgagga agacatcgat tttctcgaag cggcggacgc acgcggcaaa ctggacggct
    15421 tgcgccgcgc cgtggatgat gtgcttgcca acgcgcagca gggcgcgatt ttacgcgaag
    15481 gtctgaatgt cgtattggtc ggtgcgccga atgtcggcaa gtccagcctg ttgaacgcgc
    15541 tggcgggcga cgaagtggcg attgttaccg atattgccgg aacgacgcgc gacgcggtca
    15601 gggaacgtat cctgattgac ggcgtgccgg tgcatattgt cgatacggca ggtttgcgcg
    15661 agacggacga cgtggtcgaa cgcatcggca tcgaacgcag ccgcaaagcc gtatccgaag
    15721 ccgatgtcgc gctggtgttg gtcgatccgc gcgagggttt gaatgaaaag acgcggatga
    15781 ttttggatac gttgccgtcg gatttgaaac gcattgaaat ccacagcaaa tccgatttgc
    15841 acgcacacgc ggcaggcggg ttcggtacgg gcgcggaaac cgtcatcgcg ctgtcggcga
    15901 aaaccggcga cggcttggac gcgctgaaac ggacgctgtt gtgcgaggcc ggttggcagg
    15961 gcgaaagcga agggctgttt ttggcgcgga cgcggcacgt caacgcactc aaggcggcgc
    16021 aggaagaatt gtcgctggcc gctttgtgcg gcaaccatca aatcgagctg tttgccgaac
    16081 acttgcggct ggctcaggtc gcgtgcggcg aaattacggg ggaatttacg gcggacgacc
    16141 tgctcggcgt gattttttcg aggttttgca tcggaaaata agcgggccgg aggcattgtg
    16201 gcggtgtccg gctgaacatt ccgttatccc ataaaaacgg gaatccgatc cgttcggttt
    16261 tggttttttg aatttcgggc aacgcttaaa tcttcattcc gcgcaggcgg aaattatcgg
    16321 cgcggtacgg caactttttt caatactgaa agccccgtca ttcctgtaaa aacaaaaaaa
    16381 tcaaaaacag aaaactgaaa tatcgtcatt cccgcgcagg cgggaatcca gccccgtcgg
    16441 cacggaaact tatcgggaaa acggtttctt cagattttac gttccggatt cccgctttcg
    16501 cgggaatgac gggattaaag tttcaaaatt tattctaaat aactgaaact caacgaactg
    16561 gattcccgct tttgcgggaa tgacgaattt taggtttctg tttttggttt tctgtccttg
    16621 tgggaatgat gaaattttaa gttttaggaa tttatcggaa aaaacagaaa ccgctccg
//
LOCUS       DAXDZG010000051        16642 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXDZG010000051 DAXDZG010000000
VERSION     DAXDZG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.051"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB78_001429"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB78_001429"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC1269400.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="ACFB78_001430"
     CDS             complement(734..1447)
                     /locus_tag="ACFB78_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC1269401.1"
                     /translation="MKTKLPLFIIWLSVSASCASVSPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGKCQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="ACFB78_001431"
     CDS             complement(1447..2115)
                     /locus_tag="ACFB78_001431"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1269402.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="ACFB78_001432"
     CDS             complement(2126..2311)
                     /locus_tag="ACFB78_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC1269403.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB78_001433"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB78_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC1269404.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB78_001434"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB78_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC1269405.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFACKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="ACFB78_001434"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="ACFB78_001435"
     CDS             6730..7065
                     /locus_tag="ACFB78_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC1269406.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB78_001436"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB78_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC1269407.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB78_001437"
     CDS             8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB78_001437"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC1269408.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="ACFB78_001438"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="ACFB78_001438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC1269409.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVREQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB78_001439"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB78_001439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC1269410.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
     gene            12990..13199
                     /locus_tag="ACFB78_001440"
     CDS             12990..13199
                     /locus_tag="ACFB78_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269411.1"
                     /translation="MKLDKNNGFYQAERNSNGNYDIVRKNYLKNITKIQSNILIYLDN
                     TSTGFKIQQRRKQIRAQISARQWRR"
     gene            13791..14570
                     /locus_tag="ACFB78_001441"
     CDS             13791..14570
                     /locus_tag="ACFB78_001441"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC1269412.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            14779..15849
                     /locus_tag="ACFB78_001442"
     CDS             14779..15849
                     /locus_tag="ACFB78_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1269413.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKPQPHIRPDELPDF
                     YRALQTAHDLHPYARPLLLLAALTVPRPSALLSARWQDIDLTTRLWHIPAADMKTKHP
                     FTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYD
                     GSTPANPNTPCTDSATSSPTSA"
     gene            15810..16058
                     /locus_tag="ACFB78_001443"
     CDS             15810..16058
                     /locus_tag="ACFB78_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269414.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            16078..16341
                     /locus_tag="ACFB78_001444"
     CDS             16078..16341
                     /locus_tag="ACFB78_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269415.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            16378..>16642
                     /locus_tag="ACFB78_001445"
     CDS             16378..>16642
                     /locus_tag="ACFB78_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269416.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
BASE COUNT         4247 a         4931 c         4106 g         3358 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat tgaaatttaa tccactatat atttcagaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccacctgaac cccgaaaaca gggtttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca tttgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtgtttttgc gtgttccaat gctttgactt tgccgtttaa ggtttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtgaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 gatgcgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caattacttg gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatggc ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa atcaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcctgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atgcccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgcg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgagca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca taaaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccagcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cagacaatgg agaagataaa
    13201 agatgaataa aaatataatt tatatatttt ctttattaat cactattgtt attttcttta
    13261 tatttgaaaa gaatgtaata agcaaaataa gttttgatta taataaaaaa gaattcttaa
    13321 ttagtgatat aacgaacttt aactgggatc atgtaaaact gtatataatc aattcagatt
    13381 tccaaaaaat tgttttttat gataaaggta agatagtttt tgaggaatta atagaactag
    13441 ataggaaagg gaaagtttta cctcaatatc tatttgattc atatttgaaa aatgtttaat
    13501 attacgaatg tgattacaag aatggcaaaa tgcaactttt aaaaaagaaa aattacattc
    13561 ttttgataga tatttttatt attataaacc tataaactgt aggccgaaac ttttgtaaaa
    13621 ctgtccatac tcattaccgc ctagtcggta atgcattttt tagtagtatt tgaaaaagta
    13681 tgatgataaa aacggttaag cctaactttt tcataaaatc cagcagagta aggcagaaaa
    13741 aaacacccaa attggctata atcccgacaa acacactcaa ggacaacaac atggcagcct
    13801 cgcccgaagc aaaattcacc gaagaaaaga ttttgtgggt caaacaccac acgccgaaac
    13861 tcatcacttt cgccatcagc cgtcccgaat cctaccgttt taaagccgga cagttctccc
    13921 gactcggttt ctacgaaggt aaaggcttta tttggcgtgc ctattccgtc gtttccgcag
    13981 aatatgccga cacgctcgaa tattttgccg tactcatcca agacggcccc atgtcggccc
    14041 ttttcgccaa aatgcaacag ggcgacacca tcctgctcga taaaaatgcc actggctttc
    14101 tcctgcccga acgcttcccc gacggcaagg atttggtgat gctctgtacc ggctcgggca
    14161 tcgccccctt cctttccatt ctcgaacaac ccgaaatccg gcagcgtttc gataccgtca
    14221 acctgataca ttccgtatct tttcccgaag aattgatttt caacgaccgg ctggccgcat
    14281 tgtccgaaca tcccctggta ggcgaatacg gacactcgtt ccgtttcgtc cctgttacca
    14341 cccgtgccgc caacccctcg ggcttaagcg gaaaacgcat tccggaactc ttaaaaaaca
    14401 acagcatcga acaggcgctg cataccaagc tcaccccgga atccacacgg tttatgattt
    14461 gcggtaaccc ggaaatggtc aaagacactt tccaaacgct gctcgatatg ggttacgcca
    14521 tgcaccgcaa ccgcattccc ggtcaaatca tgatggaaaa cggcttctaa aaacgaacgc
    14581 tcccaacccc tacccacgcc cacacacaaa acaaaataaa aaacagcgat atacaaaaac
    14641 caatttgcaa aaacagacct cacgcctata ctgccactca cagcaagcaa cacaaaaaag
    14701 cgggtacaaa ttagggtaca aaacacaagc ccgaacacag aagcacccca accaaaccca
    14761 aaggcaaagg caaacaaaat ggcggcacac aacaaactga cccaaaaaca aatagaagca
    14821 gccaaggcag acggcaaaca aagcaagctg gccgacggcg gcggcttata tctgctgctg
    14881 caccccaacg gcagcaaata ctggcggatg cgctaccggc acggcggatg cgaaaaaacc
    14941 ctagccttgg gcgtatatcc cgccgtcagc ctcaagcagg cccgcgaact tgcccgcgcc
    15001 gcccgggcgc agaccgccgc cggcatcgac cccgtcgccg aacaccaccg cacccgtccc
    15061 ggcagcgggc gcagccttcc cgaaatcgcc cgcgcgtggt acggcagccg tcaagggcaa
    15121 cgcgccgaca acacactgga aggcgaccgc cgttcactcg cctacctgac cgactactac
    15181 aaagacaaca ccgacatcaa cgacatcacc accgccggcg tatcaaaatt tatcgaacac
    15241 ctcaaccgca tcaacatccc cgcctgcgcc cggcgcaccg tccaaatcct cgcccaaata
    15301 tgggactatg ccgtccagcg cggcatcatt accgacgaac ggcgcaaccc cgcaaccacc
    15361 gcccgcccac tgctaaacac aagcaaaccc aaaccccagc cccacatccg ccccgacgag
    15421 ttgcccgact tttaccgcgc cctccaaacc gcccacgacc tgcacccgta cgcccgcccc
    15481 ctgctgctgc ttgccgccct gaccgtcccc cgtccgtccg cactgctttc cgcccgctgg
    15541 caagacatcg acctcaccac ccggttatgg cacatccccg ccgccgacat gaagaccaaa
    15601 caccccttta ccgtcccact gtcggactgg gcagtagaaa tactccgaga gctgcacaca
    15661 cagaccggcg acaacatcca ccttttccca ggcatccgcc cacggcgcaa acccgccccc
    15721 catcccgacc acatcagcat caaattcgcc cacaacgcca tccgacgact gggctatgac
    15781 ggcagcaccc cggcaaatcc aaacacacca tgcacggatt ccgcgacctc ttcaccaaca
    15841 tcagcctgac cgccggaaaa gacaccctca caacagacct cgcccttggc cacatcagcc
    15901 gcgccgccct ccaacgcgcc ggcttctcaa gcctgcacca ctacctgacc gccgacagct
    15961 accgcctcca agagcggcgc gaactcgccg aatggtacgg ccgccgccac cgccaagcat
    16021 acgaagccgc cgcccaaaac acttgcaata aaacctaaaa ggtattaaca tacatacatg
    16081 gcagacatcc tgccatccga caagcggcgg caaccgccaa aaacgaaagg aaatccaata
    16141 aacataatcg aacaagaaac aaaacgcacc gagttaagaa aaatagatgc cgaaatcgcc
    16201 aaaatcattg ccgacgcgca caaaatcaac gccgaatccg taaaaatagc ccaagagtcc
    16261 cgctggtatc ccatgatggc ggccaccggt ctggttaccg ccatcgccgc cgtgttggca
    16321 ctgatattca aatttgcctg accacaagcc ccgcgagggg ctttatccct caacaaaatg
    16381 aacagagccg cactattcaa ccgctacccc gaatggatta tcgggcaaga cggcgcaagc
    16441 cgtttcatta cccattgccg ctatccccgc ctgattgcca aaatccacag gcagacagac
    16501 ggcgaatgcc cgggcgggca ttaccgccat tccgaaaacg gcatcaccct ttacgatttt
    16561 atttttttcg gcggcaaacc ggcagacgaa gcccgatttg ccgccgtcct taccgaaacc
    16621 tgccgccggg cagtcaaaaa aa
//
LOCUS       DAXDZG010000052        16545 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXDZG010000052 DAXDZG010000000
VERSION     DAXDZG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.052"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            397..1239
                     /locus_tag="ACFB78_001446"
     CDS             397..1239
                     /locus_tag="ACFB78_001446"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC1269417.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1268..1732
                     /locus_tag="ACFB78_001447"
     CDS             1268..1732
                     /locus_tag="ACFB78_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC1269418.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB78_001448"
     CDS             1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB78_001448"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC1269419.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            3068..4489
                     /locus_tag="ACFB78_001449"
     CDS             3068..4489
                     /locus_tag="ACFB78_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC1269420.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTTVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGTRGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKNLHKLFHYLKKQGFPL"
     gene            4657..5898
                     /locus_tag="ACFB78_001450"
     CDS             4657..5898
                     /locus_tag="ACFB78_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC1269421.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            6029..7204
                     /locus_tag="ACFB78_001451"
     CDS             6029..7204
                     /locus_tag="ACFB78_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC1269422.1"
                     /translation="MLNTALSPWPSFTREEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7252..9162
                     /locus_tag="ACFB78_001452"
     CDS             7252..9162
                     /locus_tag="ACFB78_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC1269423.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKYLMDGKISIGTLKKISVSDLLGRDSVASDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            9218..10510
                     /locus_tag="ACFB78_001453"
     CDS             9218..10510
                     /locus_tag="ACFB78_001453"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC1269424.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAIYCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(10562..11398)
                     /locus_tag="ACFB78_001454"
     CDS             complement(10562..11398)
                     /locus_tag="ACFB78_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC1269425.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(11693..11896)
                     /locus_tag="ACFB78_001455"
     CDS             complement(11693..11896)
                     /locus_tag="ACFB78_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC1269426.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(11874..12107)
                     /locus_tag="ACFB78_001456"
     CDS             complement(11874..12107)
                     /locus_tag="ACFB78_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC1269427.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(12119..12355)
                     /locus_tag="ACFB78_001457"
     CDS             complement(12119..12355)
                     /locus_tag="ACFB78_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269428.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB78_001458"
     CDS             12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB78_001458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC1269429.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            16116..16370
                     /locus_tag="ACFB78_001459"
                     /pseudo
     CDS             16116..16370
                     /locus_tag="ACFB78_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3749 a         5157 c         4284 g         3355 t
ORIGIN      
        1 acagcaaccc tgtcgccgtc attaccgcga aagcgggaat ccagtccgtt cagtttcggt
       61 catttccgat aaattcctgt tgcttttcat ttctagattc ccactttcgt gggaatgacg
      121 gcggaagggt tttggttttt tccgataaat tcttgaggca ttgaaattcc agattcccgc
      181 ctgcgcggga atgacggctc aaaagttacg gaacgaaaaa caaccgaaac cggacaagtc
      241 ggattcccgc ctgcgcggga atgacggaat cttaagtttc cgtctttgtt ttctgttttc
      301 gcgggaataa tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc
      361 gaatacttgc gaccatctga atcatttgga caaactatgt gtcaactgct gggcatgaac
      421 tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt
      481 accgaccatc acgccgacgg tttcggcatc ggctttttcg aaggcaaagg cgtgcgcctg
      541 ttccatgacg acaagccgag tgcaaattcc cccgtcgccg acctcgtgcg cgcctaccag
      601 attaaatcgg aaaacgtcgt cgcccacatc cgcaaagcct cgcaaggtca aacctcgctg
      661 gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac
      721 gggcatttgg tcgatttttt ccccgaacag ggagaatttt tccaccccgt cggcacaacc
      781 gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt
      841 cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc
      901 gggctgttta acttcatgct ttcagacggc atttccctgt ttgcccacgc cagcacgctg
      961 ctgcactaca tcgtccgcca agccccgttc ggcaaagcgc gcctgctcga cgacgatgta
     1021 atggtcgatt ttgccgaagt aaccacgccg gacgaccgcg tcgccgttat ctccaccctg
     1081 ccgctgactc gcgatgaatc gtggtcccaa cttgccgtga acgaactggt catgttccgc
     1141 gaaggcaaca tcgtccggca cgaccgtccc gaaaatcccg tctatatgag tgccgaagaa
     1201 ggcctggaaa tcgcccgcgc cgccggcgta tcggtttgac gttcagacgg catcaggaag
     1261 aaacaccatg aaatgcccgt tttgcagcca cccagacacc cgagttgccg attcccgcct
     1321 gatggaagag cgcaataccg tccgccgccg ccgccactgc cccggctgcg gcaaacgctt
     1381 cggcacgctc gaaaccgtcg aactcaaaat gcccgccgtc atcggtccgg acaagaaacg
     1441 ttcgcccttc aatgcacaac gcctccgcaa cgacctgacc gccgccgccc gaaaatccgc
     1501 cctgacaccc gaacagattg acgaaaccgt ccgcctgacg gaacacaggc tctacacttc
     1561 gggtcagcgc gacatatcct catccgaact tgtcgaaatc gtgctggagg agctgttcgg
     1621 gcaaaacacg gaagccgccg tccgtttcgc cgccctgcac aaacgcttcg acagcccgga
     1681 acatttcgcc tcgtggctgg cgcaagccgt caaaacaggc ggcaaaacct gattccccca
     1741 acccatactg atacggtatc cctatgtttt cggacacaaa tatatccatg atggaaaacg
     1801 ccctccgact tgccgctttg gggcgttttt ccacttcacc caacccgcgc gtcggctgcg
     1861 ttatcgcaca cggcagacaa attgtcggac agggcttcca cgtcaaagcg ggcgaacccc
     1921 atgccgaagt ccacgccctg cgtcaggcgg gggaaatggc aaaaggcgcg accgcctttg
     1981 ttaccctcga accgtgcagc cattacggac gcacaccgcc ctgcgccgaa gccctgctcc
     2041 gttccggcgt aacacgcgtc gtcgccgcca tgcgcgaccc caacccgccg gttgcaggca
     2101 aagggcttgt cctgctcaaa gcagcgggca tcaagacgga atgcggacta cttgaaaaca
     2161 aagcacgcga actcaaccgg ggatttttat cgcgcatcga acgccgccgc ccctttgtcc
     2221 gcctcaaatg cgccgtttcg ctggacggca aaaccgccct ttcagacggc agcagctttt
     2281 ggattaccgg cgaggaagcg cgtgccgacg tacaggtttt gcgtgccgaa agctgcgcgg
     2341 tgctgaccgg catcggcacg gtgttggcag acaatccccg gctcaacgtc cgcgcttttc
     2401 caactttgcg ccaacccgca cgcatcgttt tagacagccg cctgcgcctg cccccgaaca
     2461 gccatttggt taccgacgga caatctccga cctacatcgc caccttggaa cgcgatgaag
     2521 acaaactccg cccctatcgg gaacacgcac acatccgcat cctgatgccg tctgaaacgg
     2581 cagacggcaa aatcgacctg caccatctga tgcgcctcct tgccgacgaa ggtttcggcg
     2641 aaatcatggt cgaagcaggc tccgaactca catccgcatt tttggcggaa aatttggcgg
     2701 acgaaatcgt gctgtaccgt tcgcccaaaa tcctcggcgg cggcaaagac ctgttttccc
     2761 tgcccgaaaa ccgtgccgcc ctttccgcac cgcccttgtg gacacccgtt tcaagcgaaa
     2821 tcctcggaca cgacatcaaa accgtgttcc ggaaaaacgg caacgccttt taaagggttt
     2881 gccgccgttt tgctatataa taacgccgat aaaaaacggc ccgttcagac cgccgcccgc
     2941 ccggaaaagc gcaacccgga ctgccgcacc ccgccggcag ccgcgacggc ctgaaagccg
     3001 tcaaattccg atcaagaaag gcttcagacg gcacaggcag catcacgccg ccgcccggac
     3061 atcaaaaatg gacacaaaag aaatcctcgg ctacgcggca ggctcgatcg gcagcgcggt
     3121 tttagccgtc atcatcctgc cgctgctgtc gtggtatttc cccgccgacg acatcgggcg
     3181 catcgtgctg atgcagacgg cggcgggact gacggtgtcg gtattgtgcc tcgggctgga
     3241 tcaggcatac gtccgcgaat actatgccgc cgccgacaaa gacactttgt tcaaaaccct
     3301 gttcctgccg ccgctgctgt tttccgccgc gatagccgcc ctgctgcttt cccgcccgtc
     3361 cctgccgtct gaaatcctgt tttcgctcga cgatgccgcc gccggcatcg ggctggtgct
     3421 gtttgaactg agcttcctgc ccatccgctt tctcttactg gttttgcgta tggaagggcg
     3481 cgcccttgcc ttttcgtccg cgcaactcgt gcccaaactc gccattctgc tgctgttgcc
     3541 gctgacggtc gggctgctgc actttccggc gaacaccgcc gtcctgacca ccgtttacgc
     3601 gctggcaaac cttgccgccg ccgccttttt gctgtttcaa aaccgatgcc gtctgaaggc
     3661 cgtccggcgc gcgccgtttt cgcccgccgt cctgcaccgg gggctgcgct acggcatacc
     3721 gctcgcactg agcagccttg cctattgggg gctggcatcc gccgaccgtt tgttcctgaa
     3781 aaaatatgcg ggcctggaac agctcggcgt ttattcgatg ggtatttcgt tcggcggggc
     3841 ggcattattg ctccaaagca tcttttcaac ggtctggaca ccgtatattt tccgtgcaat
     3901 cgaagaaaac gccacgcccg cccgcctctc ggcaacggca gaatccgccg ccgccctgct
     3961 tgcctccgcc ctctgcctga ccggaatttt ctcgcccctc gcctccctcc tactgccgga
     4021 aaactacgcc gccgtccggt ttaccgtcgt atcgtgcatg ttgccgccgc tgttttacac
     4081 gctgaccgaa atcagcggca tcggtttgaa cgtcgtccgc aaaacgcgtc cgatcgcgct
     4141 tgccaccttg ggcgcgctgg cggcaaacct gctgctgctg gggcttgccg taccgtccgg
     4201 cggcacgcgc ggcgcggcgg ttgcctgtgc cgcctcattc tggttgtttt ttgttttcaa
     4261 gacagaaagc tcctgccgcc tgtggcagcc gctcaaacgc ctgccgcttt atatgcacac
     4321 attgttctgc ctggcctcct cggcggccta cacctgcttc ggcacaccgg caaactaccc
     4381 cctgtttgcc ggcgtatggg cggcatatct ggcaggctgc atcctgcgcc accggaaaaa
     4441 tttgcacaaa ctgtttcatt atttgaaaaa acaaggtttc ccattatgaa aatcatcctg
     4501 actacttcta tgtcggttct gggaggcacg ggcacgacat catcgattgc caaatgtcgt
     4561 gcaaaaccga ccccgttcag tccgacgaaa tcgtccgccg cttcaggcgc gacatttcct
     4621 atcggaaaat cgtcaacctg attgaaagat tggcaaatga gtaaagccgt caaacgcctg
     4681 ttcgacatca tcgcatccgc atcggggctg attgtcctgt cgcccgtgtt tctggttttg
     4741 atatacctca tccgcaagaa tctgggttcg cccgtcttct tcattcggga acgccccgga
     4801 aaggacggaa aaccttttaa aatggtcaaa ttccgttcca tgcgcgacgc gcttgattca
     4861 gacggcattc cgctgcccga cagcgagcgg ctcacgccct tcggcaaaaa actgcgcacc
     4921 gccagtttgg acgaactgcc tgaactgtgg aacgtcctca aaggcgagat gagcctggtc
     4981 ggcccccgcc cgcttttgat gcagtatctg ccgctttaca acaaatttca aaaccgccgc
     5041 cacgaaatga aaccgggcat taccggctgg gcgcaggtca acgggcgcaa cgcgctttcg
     5101 tgggacgaaa agttctcctg cgatgtttgg tacaccgaca atttcagctt ttggctggat
     5161 atgaaaatcc tgtttctgac agtcaaaaaa gtcttgatta aagaaggcat ttcggcgcaa
     5221 ggggaagcca ccatgccccc tttcgcgggg aatcgcaaac tcgccgttat cggcgcgggc
     5281 ggacacggca aagtcgttgc cgagcttgcc gccgcactcg gcacatacgg cgaaatcgtt
     5341 tttctggacg accgcaccca aggcagcgtc aacggcttcc ccgtcatcgg cacgacgctg
     5401 ctgcttgaaa acagtttatc gcccgaacaa ttcgacatca ccgtcgccgt cggcaacaac
     5461 cgcatccgcc gccaaatcac cgaaaaagcc gccgcgctcg gcttcaaact gcccgttctg
     5521 attcatcccg acgcgaccgt ctcgccttct gcaataatcg gacaaggcag cgtcgtaatg
     5581 gcgaaagccg tcgtacaggc cggcagcgta ttgaaagacg gcgtgattgt gaacactgcc
     5641 gccaccgtcg atcacgactg cctgcttgac gctttcgtcc acatcagccc gggcgcgcac
     5701 ctgtcaggca acacgcgtat cggcgaagaa agccggatag gcacgggcgc gtgcagccgc
     5761 cagcagacaa ccgtcggcag cggggttacc gccggtgcag gggcggttat cgtatgcgac
     5821 atcccggacg gcatgaccgt cgcgggcaac ccggcaaagc cccttacggg caaaaacccc
     5881 aagaccggga cggcataagc gattaaaata aacttccgta caaaccgatt ttgacaacac
     5941 ctgcggcgcg cgcccgattc ttcggaacac gcccccttca gacggcatag ggtcggaaat
     6001 gccgtccgaa cccgacggac caaacaccat gctgaacacc gccctttccc catggccgag
     6061 cttcacccga gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta
     6121 ctggacgggc agcgaatgcc gcgaatttga aaaagaattt gccgcctttg ccggcacgcg
     6181 gtacgccgtc gccctttcca acggcacgct tgcgctcgat gccgcactca aagcaatcgg
     6241 cataggcgcg ggcgacgatg tgattgttac ctcgcgcacc ttcctcgcct ccgcctcctg
     6301 cattgtgaac gcgggcgcaa accccgtgtt tgccgacgtg gatttgaaca gccaaaacat
     6361 cagcgcggaa accgtcaaag ccgtgctgac cccgaatacc aaagccgtca ttgtcgtcca
     6421 ccttgccggt atgcctgccg aaatggacgg catcatggct ttggcaaaag aacatgattt
     6481 gtgggtgatt gaagactgcg cccaagcgca cggcgcgacc tacaaaggca aatccgtcgg
     6541 ctctatcggg cacgtcggcg cgtggtcgtt ctgtcaagac aaaatcatca ccaccggcgg
     6601 cgagggcggt atggttacga ccaacgacaa aaccctgtgg gaaaaaatgt gggcgtacaa
     6661 agaccacggc aaaagctacg atgccgtgta ccaccgcgaa cacgcgcccg gtttccgctg
     6721 gctgcacgaa agtttcggca ccaactggcg catgatggaa atgcaggctg tcatcggccg
     6781 catccagctc aaacacctgc ccgaatggac ggcgcgccgc caagaaaacg ccgccaagct
     6841 ggcagaaagt ttgcgcaaat tcaaaagcat ccgcctgatt gaagtcgccg gctacatcgg
     6901 acacgcgcaa tacaagttct acgccttcgt caaacccgaa cacctgaaag acgactggac
     6961 gcgcgaccgc atcgtcagcg aactgaacgc gcgcaacgtc ccctgctatc aaggcggctg
     7021 ctccgaagtg tatttggaaa aagccttcga caacacgccg tggcggccca aagagcggct
     7081 gaaaaacgcc gtcgaactgg gcggcaccgc cctgacgttc ttggtgcacc cgacgctgac
     7141 cgacgacgaa atcgcgttct gcaaaaaaca catcgaagcc gtcctgaccg aagccgcacg
     7201 ataacccttc agacggcata tgccgcctga aagcacatac cgccccacga tatgaatctg
     7261 gaaaccctga tcgccctgcc gcgcaacatc aagaaaatct gtttcctcat acacgatttt
     7321 ctgatgattt tcattgcctt ttggttcacc caaagcctga aggccgacta ctcggacgaa
     7381 tggttcgatt ttgccaactg gcagtctttt ttgctgactg ccttgctgac catcacatta
     7441 tttatccgaa tggggcttta ccgcgccgtt acacgctttg tcagtttcca cgtcctgacc
     7501 accgcctttg ccggcagcct cgcctccgcc gtattgttct tcctcaacac attgattttt
     7561 gaagaaaggc tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg
     7621 accggctcgc gtatggtttt gcgcggactc ctgtccgacc accagaaaaa aacacatgac
     7681 cccgtcatca tttacggcgc ggggcgttcg ggtcggcagc tccttgaagc cgtcaaacag
     7741 atgcgcgaat attccgccgc cgcctttgtg gacgacaacc ccaagctttg gcacaccgtc
     7801 atctacgacc ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc
     7861 gtggaaaaaa tcctgctcgc cattcccagc gcgactcagg aacaacgccg ccgaatcatc
     7921 agcaaactga aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa atacctgatg
     7981 gacgggaaaa tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgc
     8041 gattccgtcg catccgacga ccgcctcatg aatgccgaca ccgaaggcaa aaccgtcatg
     8101 gtaaccggcg cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc
     8161 cccgaaaggc tgctgctgtt cgagctgtcc gaattcgccc tgtacaccgt tgaaaaagaa
     8221 ttgtgcgaat actgcgcccg aaacggcatc gccgccgaaa tccttccctt tttgggttcg
     8281 gtgcaaaacc gcacgctgct cacgcacatt atgaccgcct tttccgttgc gaccgtctat
     8341 cacgccgccg cctacaaaca cgtccctatg gtcgagttca acaccgtcga gggcatacgc
     8401 aacaacatct tcggcacact cgaatgcgcg cttgccgcca cggcttcggg cgtggaaacc
     8461 ttcgtcctca tctccaccga caaagccgtc cgccccgcca acacgatggg tgcgggcaaa
     8521 cgcatggcgg aactctgcct tcaggcactc gccgccgaac ccggacaaaa aacccgcttc
     8581 agcatggtac gtttcggcaa tgttttaggc tcgtccggct ccgtcgtccc gctgtttgaa
     8641 aaacagattg ccgaaggcgg ccccatcacc ctgacccacc ccgacatcac gcgctatttc
     8701 atgaccatac ccgaagcggc gcaactggtt atccaagccg gcgcgatggg cagaggcggc
     8761 gacgtgttcg tcctcgatat gggcgaatcc gtcaaaatca ccgaccttgc ccgccaaatg
     8821 attaccttaa gcggactcaa acccaaaacg cccgaacagc ccgacggcga catcgaaatc
     8881 ctcattaccg ggctgcgccc cggcgaaaaa ctctacgaag agttgctcat cggcgacaac
     8941 gtccgcaaaa ccggccatcc gcgcatcatg accgccgacg agaccatgct gccgtggcac
     9001 gaactctccg ccctgctcga ccgcatccgc accgcctgcg accgttacga ccagcaggca
     9061 atccgcgacc tgctcgtcaa cgccccgacc ggctttaccc cgaccggcgg catctgcgac
     9121 ctgctttggg tatgcgaaac acacagaaaa aatgccgtct gaaccttcag acggcatacc
     9181 gtacaaacca atctgcctta cacacactga gtttgacatg cagttctcgg cattcggcga
     9241 aaaattcacg caacacagcg gcatcctgca actgatggac gacctcggcg acgcgctcaa
     9301 aagcgacaag cccgtcaata tgctcggcgg cggcaacccg gcgcgcattc ccgaaatcga
     9361 tcgggcgttc gccgacatat tttccaagct ggcggcggaa cacgccgtcg agaacatcgg
     9421 caactactcc aatccccaag gcgatgccgc gctgattgac gcgctgaccg ccttccccaa
     9481 ccgcgaatac ggctggaacc tgaccgtcgg caatatcgcg ctgaccaacg gttcgcaaaa
     9541 cgcgtttttc tatttattca acctcttcgg cggcaaattc aagctttcag acggcacatc
     9601 cgcagaaaaa gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca
     9661 tgtcgaaggg cggcacttcg tttccgtcaa acccaaaatc gaaaacgtcg aacacgaagg
     9721 cgaagcaggc ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact
     9781 caaagcgggc aaaatcggcg cgatttactg ttcgcgcccg accaacccga ccggcaacgt
     9841 gttgaccgac ggcgaaatgg cgcgtttgga cgctttggcg cgtgaacacg gcattccgcc
     9901 gattatcgac aacgcctacg gaatgccgtt ccccaacatt attgacagcg gcgtaacgct
     9961 gaattggcac gaaaacatca tcctctgctt cagcctttcc aaagtcggcc tgccgggcgt
    10021 tcgcaccggc atcatcgtcg ccgcgcccga agccgtcaaa gccgtcagca gcctgaacgc
    10081 gattgtgaac cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tgctggaaag
    10141 cggcgagatg aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca
    10201 aaccgccgtt tcgctgctca agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc
    10261 cgaaggcgcg attttcctgt ggctctggtt tgaaaacctg cccgtttcgt cgcaaaccct
    10321 gtacgaaatg ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg
    10381 catcgacacg caagactacc cgcacgcggg cgagtgcatc cgtatgagta tcgcacagga
    10441 cgctcaaacg ctggaaaaag gcatcgccgc catcggcaaa accgtccgaa aaccgtacga
    10501 caacgtttaa aacgcaaaaa atgccgtctg aagacctttc agacggcatt ttcatccgca
    10561 ttcaatgtcg ggaaaaatgc gcccgcaccg gtttgcagtt ttccggaaga tcgggacacg
    10621 cgccgaggat gccgccgctg aagtcgtcta aacggtggaa gtcgctgccc gcgcttgcca
    10681 acaatccgaa gcggtctgcc aaaagcgcgt agttgaggcg gtcgtttttg cagcagttgc
    10741 cgctgtggac ttcgatgccc gcgccgccga ggtttttaaa ttcttcaaac aggttgcgct
    10801 tggcggttgc cgacaaatca taacgcatcg gatgggcaat caccgccatg ccgcccgcgc
    10861 cgttgacggc ggagacgcag tctgccagcg tcgcccattc gtggcgtacc gcgcaggatt
    10921 tgccgtcgcc caagtatttg gtgaacgcct gctgcttgtt tttgacgtgt ccgtttttga
    10981 tgaggaactc ggcgacgtgg gtgcggctga ccatttcttt gtttgccgcc agcgccagcg
    11041 cgccgtcgta tgcgccgccg atgcctttct tttcgagctt ggcggcgatg gcttcaagac
    11101 gtttcagacg gcctttgcgg acatctgcca acaggttttg caggttttcg tcctgctcgt
    11161 cgaaatccaa accgacaacg tgtatggtac gcccgcgcca cgttaccgaa atttccacgc
    11221 cgttaatcag gcgcaaaccg agcctgtcgg cttcggcacg cgcttcggag atgccgcccg
    11281 tgtggtcgtg gtcggtcaac gccagcagcg tgcagccgtt ttgatgtgcg aggcggacga
    11341 cttcggcggg ggggagcata ccgtcggaaa cggtggaatg gcagtgcagg tctatcatgg
    11401 ggtgttatgt ggtggtgaat gaaggacggg atgtttcaat gttgtcggac agtgtggcgg
    11461 cggcggtcat tttaaaaaac aggtgtatgg caaatgcaaa ggaaaagccg ctatgccgtc
    11521 attcccgcgc aggcgagaat ccagactttg atttattgga aatgtttaag gttaaccgct
    11581 atttcaaact tccggattcc tgctttcgag ggaatggcga tatgaacgtt ttcaatttta
    11641 atctactata aaagaccgtc tgaaaacgtg gttttatcaa gcccaaccgt tttcagacgg
    11701 catcgtccgg ccaacccgct tccttcagtt ttttccgttg cgcatcgtag cgtgccttgt
    11761 ccgcccagta aatcgtctga atgcacgcct cgccgcagtc gctgccgcag cattcccacg
    11821 actcggggcg gacgggttcg tccaaaagcg gctcgcccaa aagggcttcg gctttatgct
    11881 tcagggtcgt atccatcggc gatttccaag cgcgcgccgt caaactcgat gacttctccg
    11941 ccgcgtattt tggccgtttt gcggatttcg gttccgccgt tgcgcaacac cagcccttcg
    12001 gcgataaatg cttttgcctg tccgccgctt tcggcaagtc cggccaattt caagaggtcg
    12061 cacaaggcga tgtattcgtt gtcttcgaga tagacggtgg cttccataat gtcccctttt
    12121 agaaagaggg cgttattgta gcacctgccg ccgccgcacc caaaattgcc gcaaaaccgg
    12181 cgatgtgtcc gcactgcctg ttccgtaaaa atgccgtctg aaaccccgta tgccgccatc
    12241 cgtccaaaga aatcctgccc aacggcaggc tgcaaatcct gttccccgac gaatccgcat
    12301 tgacgctgat gcacatcctc aaacgcggac tgcccgatac accggcaatc ggcatcaaaa
    12361 cgaaatcaaa aacctgtttg aacgtaaaat ctatttaact gcttgccgtc cgtccgaata
    12421 aggtatatag ttccttataa ctattttgat agtcctttat atctatcaat actccttggg
    12481 aagcctccgc cacacggcag gaggcatttt tttgccgtcg taaaagctca aaaaacattt
    12541 gcaggtcatg ccgtctgaat ccgaaacggc attacctgca ccgccatcta aagacaaccc
    12601 tgctacaata cgccttttat tgtccacgcc gattttgcca tgaccgagcc gacctacatc
    12661 cccctgcgcc tgcataccga attctccatt accgacggta tggtgcggat taaaaaactg
    12721 attgccaaag cgcaagaata cggtttgcct gctttgggca tcagcgattt gatgaacgaa
    12781 ttcggtttgg tgaaattcta taaagcctgc cgcggcgcag ggattaagcc cgtcggcgcg
    12841 gcggacgtgt ggataggcaa tccgaatgcg cccgacaagc cgttccgcgc catgctggtt
    12901 atccgcaacg atgcgggtta tctgcgcttg agcgagcttt tgacggaggc ttatgtcggc
    12961 caagaccgca atatccatca tgcggaactc aatcccgaat ggctggaaaa cggcgacaac
    13021 agcggcttga tttgtttgag cggcgcgcat tacggcgaag tgggcgtgaa tctgttgaac
    13081 ggcaatgaag acgcggcgcg tgcggcggcg ttgaaatatg cggcatggtt tcccgatacg
    13141 ttttatttag agctgcaacg cctgcccgaa cgccccgaat gggaggcttg cgtttccggc
    13201 agcgtgaagc tggcggagga actgggtttg ccggtggtag caacgcatcc gacacagttt
    13261 atgaaccgcg acgatttcaa cgcgcacgag gcgcgggtgt gcatcgcggg cggctgggtg
    13321 ctgacggaca agaaacgtcc gcgcgatttc acgccgagcc agtttttcat tccgccggaa
    13381 acgatgctgg agcgtttcgc cgacttgccc gaagccttgg aaaacacggt ggaaatcgcc
    13441 aaacgctgca acttgcacat cacattgggc aaaaacttcc tacccctttt ccccacgccc
    13501 gacggtttat cactcgatga ctgtctcatc aaactctcca acgagggttt gcaggaacgc
    13561 atggttcagc tttatcccga cgaggcggaa cgggcggcaa aaatgccgga atatcaggag
    13621 cggctggact ttgagctgaa catcatcatc caaatgaaat tccccggcta tttccttatc
    13681 gtacaagact ttatcaactg ggcgaaaacg cacggctgtc cggtcgggcc gggacgcggt
    13741 tcgggcgcgg gttcgctggt ggcgtattcg ctgaagatta ccgacctcga cccgctgaaa
    13801 tacgcgctgc tgttcgagcg tttcctaaac cccgaacgcg tttccatgcc cgactttgac
    13861 gtggacttct gtcaggcaaa ccgcggccgc gtgattgaat acgtgcgcga aaaatacggc
    13921 gctcaggcgg tcagccagat tgtgactttc ggcacgatgt cgtccaaagc ggtcatccgc
    13981 gacgtggggc gtgtgctgga actgccgttt atgctgtgcg acaaactgtc caagctgatt
    14041 ccgttggaag ccaacaaacc cctgagtttg gacgacgcga tgaaggcgca gccgcagatt
    14101 caggaattgc ttgaagcgga agaagcggac gaactgatta cgctggcgaa aaaactggaa
    14161 gacctgacgc gcggtttggg tatgcacgca ggcggcgtgt tgattgcgcc gggcaagatt
    14221 tccgattaca gccccgtgta tcaggcggac gaatccgcct cgcccgtatc catgtacgac
    14281 aagggcgacg tggaagacgt gggtttggtg aagttcgact ttttgggcct gcgcaacctg
    14341 accattatcg aaatggcgca gaacaacatc aaaaacaccg ccggcgatat cgtcgatgtc
    14401 ggcaagattc cgcttgacga ccagactgcc taccaaatct tccgcgatgc gaacaccacc
    14461 gccgtcttcc agttcgagtc gaccggcatg aaaaaaatgc tgaaaacggc gcatacgacc
    14521 aagtttgaag aactcatcgc cttcgtatcg ctctaccgac ccggcccgat ggacaacatt
    14581 cccgacttcg tcgcacgcat gaagggacag gaattccaat acatccaccc gctgctggaa
    14641 ggcatcctcg cgccgaccta cggcattatg gtgtatcagg aacaagtgat gcaggcggcg
    14701 cagattatcg gcggctactc gctcggcggc gcggacctgc tgcgccgcgc catgggtaag
    14761 aaaaagcccg aagagatggt gaaacaccgc gaaatcttcg ccgaaggtgc ggcaaaacaa
    14821 ggcatttcgc gcgaaaaatc cgacgaaatc ttcaactaca tggaaaaatt cgccggctac
    14881 ggtttcaaca aatcccacgc cgccgcctac gccctgattt cctaccagac cgcatggctc
    14941 aaagcccact atcccgccga atttatggcg gcgacgatgt cgtccgaatt ggacaacacc
    15001 gaccagctca aacatttcta cgacgactgc cgcgccaacg gcatcgagtt cctgccgccc
    15061 gacattaacg aatccgacta ccgcttcacg ccgtatccga acatgaaaat ccgctacgca
    15121 ctcggcgcga ttaaaggcac gggcgaagcc gccgtcgaat ccatcatcgc cgcgcggcaa
    15181 agcggcggca aatttaccgg cctgttggac ttctgcgagc gcgtcggcaa agaacacatg
    15241 aaccgccgca ccctcgaagc cctgatacgc ggcggcgcgt tcgacagcat cgaacccaac
    15301 cgcgccatgc tcttggcgaa catcgacctt gccatggaca acgccgacca aaaagccgcc
    15361 aacgccaatc agggcgggct gtttgacatg atggaagacg ccatcgaacc ggtgcggctc
    15421 atcgacgcac ccatgtggag cgaatcggaa aaactcgccg aagaaaaaac cgtcatcggc
    15481 ttttacctgt ccggccaccc gttcggcccg tatgcccaag aagtccgcca aatcgccccg
    15541 accaaattag gccgtctgaa accgcaagac agcgtgcgcc tcgccggatt cgttaccgcc
    15601 gtgcgcacca tgatgggcaa acgcggcaaa atcgccttcg tcagcctcga agattcgagc
    15661 gggcaggctg aaatcatggt cggcggacaa acgctggaaa actgcgccga ctgcctcaaa
    15721 gccgaccaag tgctgattat cgaatccaaa gtcagccgcg acgattacgg cggcggcgac
    15781 gggctgcgca tcatggcaaa ccaagtcatg accctgcaaa cggcgcgcga acgctacgcc
    15841 cgcagcctca gcctcgccct tgccccgcat cacgacatcg gcggactggt acggctgctc
    15901 gccgcccacc aactgcccga cacgccgcgc atcccgcttc aattgtcgta tgccaacgaa
    15961 aaagcgtcgg gcaggcttca agtgcctccg aaatggacgg ttacaccgag ttccgcattg
    16021 ttcggcgaac tggaaacatt gctcggcagc cggtcggtgc gcgtgaattg gtaaccaaaa
    16081 atacaaatgc cgtctgaagc ccaaaaccgg tttcattcgt actttattcg aatgattgaa
    16141 taaaagtaac tgccaagaaa aacgtatttt ttggttattt cgccagccta aataaatcaa
    16201 ccggggcgat tgatatccgt gtacatgaca cagacagcgc caaagggaaa aacggcattt
    16261 tcaaaagtat cggtgtcaaa accgcccttt cattccaaaa ataccaaatc gacgaaccgg
    16321 gcaaagaaat cagaccgtgc cgcctgaaaa aacgcccgcc cgtccgttaa attaatctat
    16381 ccctgtttca gacggcctga agcagggatt tttatatcaa gataaaatga gaacgggagc
    16441 gacaaccacg ggcaaagtcg gcgcgaacta tcaaaaattg tagcactgcg aaatgagaac
    16501 gggagcaata acccttaggt agctcttgtt atagcggatt aacga
//
LOCUS       DAXDZG010000053        16347 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXDZG010000053 DAXDZG010000000
VERSION     DAXDZG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16347
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.053"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            294..1622
                     /gene="miaB"
                     /locus_tag="ACFB78_001460"
     CDS             294..1622
                     /gene="miaB"
                     /locus_tag="ACFB78_001460"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC1269430.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            1640..1804
                     /locus_tag="ACFB78_001461"
     CDS             1640..1804
                     /locus_tag="ACFB78_001461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269431.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(1869..3782)
                     /gene="dxs"
                     /locus_tag="ACFB78_001462"
     CDS             complement(1869..3782)
                     /gene="dxs"
                     /locus_tag="ACFB78_001462"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC1269432.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(3867..4784)
                     /gene="xerC"
                     /locus_tag="ACFB78_001463"
     CDS             complement(3867..4784)
                     /gene="xerC"
                     /locus_tag="ACFB78_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC1269433.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4978..6042
                     /gene="fba"
                     /locus_tag="ACFB78_001464"
     CDS             4978..6042
                     /gene="fba"
                     /locus_tag="ACFB78_001464"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC1269434.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            6181..7038
                     /locus_tag="ACFB78_001465"
     CDS             6181..7038
                     /locus_tag="ACFB78_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC1269435.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGGRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            7177..7854
                     /gene="tsaB"
                     /locus_tag="ACFB78_001466"
     CDS             7177..7854
                     /gene="tsaB"
                     /locus_tag="ACFB78_001466"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC1269436.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            7851..8291
                     /gene="rimI"
                     /locus_tag="ACFB78_001467"
     CDS             7851..8291
                     /gene="rimI"
                     /locus_tag="ACFB78_001467"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC1269437.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     TEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            8285..9022
                     /locus_tag="ACFB78_001468"
     CDS             8285..9022
                     /locus_tag="ACFB78_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC1269438.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            9084..9725
                     /gene="pyrE"
                     /locus_tag="ACFB78_001469"
     CDS             9084..9725
                     /gene="pyrE"
                     /locus_tag="ACFB78_001469"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC1269439.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GKLSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            9793..10221
                     /locus_tag="ACFB78_001470"
     CDS             9793..10221
                     /locus_tag="ACFB78_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC1269440.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            10218..11528
                     /gene="argA"
                     /locus_tag="ACFB78_001471"
     CDS             10218..11528
                     /gene="argA"
                     /locus_tag="ACFB78_001471"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC1269441.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            11624..13639
                     /locus_tag="ACFB78_001472"
     CDS             11624..13639
                     /locus_tag="ACFB78_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC1269442.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(13752..14708)
                     /locus_tag="ACFB78_001473"
     CDS             complement(13752..14708)
                     /locus_tag="ACFB78_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1269443.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIKSGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14944..15915
                     /locus_tag="ACFB78_001474"
     CDS             14944..15915
                     /locus_tag="ACFB78_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1269444.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHSD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            15948..16271
                     /locus_tag="ACFB78_001475"
     CDS             15948..16271
                     /locus_tag="ACFB78_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC1269445.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         3665 a         5036 c         4453 g         3193 t
ORIGIN      
        1 ggtttaaatt taatccacta taacgcagga actgatgttc cctgtcgccg aaattgctgg
       61 tacacgcaca cagcagcaat gccgcccata cacacacctc ccgttaaagc caaacattat
      121 acagccgttc cgaccgatta aattcatatt ttaaaacaat attttgcctc ccaaacccac
      181 atcgtgctat aatccgcacc gattttcaga cggcatcgtc gtgccgtctg aaattttttc
      241 attccaacaa ccatcagccc cgcgattacg gctgcctgag aaagacacaa accatgaaaa
      301 aagtatttat ccgcaccttc ggctgccaga tgaacgagta cgacagcgaa aaaatgctgt
      361 ccgtccttgc cgaagaacac ggcggcatcg aacaggttac ccaagccgac gaagccgaca
      421 tcatcttgtt caacacctgt tccgtgcgcg aaaaagcaca ggaaaaagtg ttctccgact
      481 tggggcgcgt ccgtccgctc aaagaaaaaa accccggcct catcatcggc gttgccggct
      541 gcgtcgcctc gcaagaaggc gaaaacatca tcaaacgcgc gccttatgtt gacgtggttt
      601 tcggtccgca aacgctgcac cgcctgccca aaatgattgt ggacaaagaa accagcgggc
      661 tgtcgcaagt cgatatttcc ttccccgaaa tcgaaaaatt cgaccacctg ccgcccgccc
      721 gcgtcgaagg cggcgcggca tttgtgtcga ttatggaagg ctgttccaaa tactgctcct
      781 tctgcgtcgt cccctacacg cgcggcgaag aattctcccg cccgctcaac gacgtattga
      841 ccgaaatcgc caaccttgcc cagcaaggcg tgaaagaaat caacctcttg ggacaaaacg
      901 tcaatgccta tcgcggcgaa atggacgacg gcgaaatctg cgacttcgcc accctgctgc
      961 gtatcgtcca cgaaatcccc ggcatcgaac gtatgcgctt caccaccagc cacccgcgcg
     1021 agtttaccga ctcgattatc gagtgctacc gcgacctgcc caaactggtt tcccacctgc
     1081 acctgccgat tcaaagcggt tccgaccgcg tattgagcgc gatgaaacgc ggctacaccg
     1141 ctttggaata caaatccatc atccgcaaac tgcgcgccat ccgtcctgat ttgtgcctga
     1201 gcagcgactt catcgtcggt ttccccggcg agaccgaacg cgagttcgag caaaccttga
     1261 aactggtgaa agacattgcc ttcgacttga gcttcgtgtt tatttacagc ccgcgccccg
     1321 gcacgcctgc cgccaacctg ccggacgaca cgccgcacga agaaaaagtg cgccgcctcg
     1381 aagccttgaa cgaagtcatc gaagccgaaa ccgcccgcat caaccaaacc atgatcggca
     1441 cggttcaacg ctgcctggtc gaaggcatct ccaaaaaaga ccccgaccag ctccaagccc
     1501 gtaccgccaa caaccgcgtc gtcaacttta ccggcacgcc cgacatgatc aaccaaatga
     1561 tcgatttgga aatcaccgaa gcctacacct tttccctgcg cggcaaaatt gtcgaagcct
     1621 aaaaccctca cgccgaaaaa tgccgcctga agcgtttcag acggcatttt gccttgtatc
     1681 ggcagacggc accgccgccg ttatacggac aagcggaata aatttccaaa cgcgcaacgg
     1741 cacaccaagc accgcccgaa agccgccgcc cgaacccttc ctgccgcctg cggggtttca
     1801 ataaaaaaac cgcagaaaca aacgccgttt aaaaacgccg cccgataacg acggcgcggt
     1861 caagcggttt aatttgccgc atcacggtcc ggcagccact cgcgcacccg gcgttccacc
     1921 gcttcggcac tcaaacccaa atcgtccaaa agttttttcg gatcgccgtg ttcggttacg
     1981 gtatcggcaa cgcccaaaag caaaacgggt ttgcagatgc cgtgtttcgc caacacttcc
     2041 aagaccgcgc cgcctgcgcc gccctgttcg gcgttttctt caagggtaac gatgcggtcg
     2101 tggcttcggg caaggcggac aatcaactct tcgtctatcg gtttgacgaa gcgcatatcg
     2161 gcgacggtgg cgttcagttt ttcggcaacc gccaatgcgg gggcgaccat actgccgaag
     2221 gcaatgaagg cggttttctc accttcgcgg cggataatgc ccttgccgat ttccacggtt
     2281 tccatgccgt ctgaaaccgg cgcgcccgta cccgtgccgc gcggatagcg gacggcggcg
     2341 ggcgcatccg cctgatagca ggtcgaaagc agcaggcggc attcgttttc atcgctcggc
     2401 gcggcaacaa tcatgttcgg cacacagcgc aagaagctca aatcgtacaa gccggcatgg
     2461 gtcgggccgt ccgcgccgac gatgcccgca cggtcgacgg caaacaaaac gggcaggttt
     2521 tgcagggcga tgtcgtgcac cagttggtcg taggcgcgtt gtaaaaaggt ggaataaatc
     2581 gccacgacgg gcttcatgcc ttcgcacgcc aaaccgccgg caaaggtaac ggcgtgctgc
     2641 tcggcgatgc cgacatcgaa atagcggtcg gggaatcgtt gttcaaactc caccagtccg
     2701 ctgccctcgc gcatggcggg ggtaatcgca accagtcggg aatctgccgc cgcccggtcg
     2761 cacagccatt tgccgaatac ttgggtatag gtcggtttgg cggcgggctt gggttctttt
     2821 tcagacggca tttgcgcccc gccttcttta ggcaggttgg cgacggcgtg gtatttgacg
     2881 gggtcgtttt cggcgagttt gtagccgttg ccctttttgg tgatgacgtg cagcaactga
     2941 gggcctttgc ggctgcgcaa gtctttcaat acgtccacca gattctcgac gttgtgtccg
     3001 tccacggggc cggtgtagcg gaagccgaaa ttttcaaaca gcgacagcga ctgtttggcg
     3061 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     3121 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacagtccg
     3181 tgcatatcgc gcacgacgtt gctggcaaga tatttgggca acgcgccgac gttgggggaa
     3241 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcccgcgcaa
     3301 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3361 cggcggtcgc cgcccaagag tttgtctgcc gccgccatgc ccaaagccgc gccgatggag
     3421 gtggaggaat gccccacgcc gaacgcgtcg tactcggact cgcaacgttt cggaaaaccc
     3481 gccaaaccgc cgtattggcg catggtgtgc atctggtttt tcctgcctgt caggattttg
     3541 tgcgggtagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt gtcgtacaca
     3601 tagtgcaggg cgatggtcag ttcgaccgca cccagattgc tggcgaaatg cccgccggtc
     3661 tgcccgacag attccagcag aaaggcgcgc aactcgccgg caaggcgcgg cagctgtttt
     3721 ttgtccaggc ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3781 atggtgtgtc ttttttatgt gtcgtccggg tgcaacggtc aattatatat caagagcgtg
     3841 cggctgacgg ctgattttgc cgtacgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3901 tcgtcataga ggcgggcgat gtggtcgaaa tcgagcttgg tataaatctg cgtggtcgaa
     3961 aggctgctgt gtccgagcag ctcctgcacc gccctgatgt cgcgcgaagc ctgcaacagg
     4021 tggccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgtattgc
     4081 gccgcccacg attcgaggcg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     4141 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcaag
     4201 gcttccacgc ttttgccggt cagcggaacc tgacgctgct tgcgcccttt gccggtaacg
     4261 tgtacccacg cttcgtccaa atatacatca tctgcattca agccgtgtat ctcgctcacg
     4321 cgcaaaccgc tgccgtacat cagctcgaac agcgcgtggt cgcgcaccgc cagcgggtcg
     4381 ccgccgtcca cgggcaaatc caacatccgg ttcagccatt cctgcggcag ggctttgggt
     4441 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggcgcg catcagcccg
     4501 cgtttgacca gccaggcgca atactgccgc cacgccgaca gcttgcgcgc cagcgtccga
     4561 ccgtccaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ccgcgacaag
     4621 tcctgcggca cgccgcctgc atcttcagac ggcatttgtg ccaacagtgc aaacagttct
     4681 tccaaatcgc gccggtatgc cgcaaccgtg tgctccgact tgccctcgcg cacgatgttt
     4741 tccaaataag cgtcaaaata cgccgcaaac ccgtccaaaa ccataaccgt cccacacaaa
     4801 tatcaaaaaa ccagtgaaaa cattatcata aacaacacac caaaatcccg aaccgcccaa
     4861 aatccgcccc atctgcccgc cgttatcgtt aatataaaat caatgagccg tttatggttt
     4921 tttgccgtaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4981 gcactcgtat ccatgcgcca actgcttgac cacgccgccg aaaacagcta cggcctgccc
     5041 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     5101 aacgcgcccg tcatcgtaca ggcgagcgca ggtgcgcgca aatacgcggg cgcgccgttt
     5161 ttgcgccacc tgattctggc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5221 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttctcctcc
     5281 gtgatgatgg acggctcttt gctcgaagac ggcaaaaccc cttcttctta cgaatacaac
     5341 gtcaacgcca cccgtaccgt cgtcaacttc tcccacgcct gcggcgtgtc cgtcgaaggc
     5401 gaaatcggcg tattgggcaa cctcgaaacc ggcgaagcag gcgaagaaga cggagtgggc
     5461 gcggcaggca aactctcaca cgaccaaatg ctcaccagcg ttgaagatgc cgtgcgtttc
     5521 gttaaagata ccggcgttga cgcattggcg attgccgtcg gcaccagcca cggcgcatac
     5581 aaattcaccc gtccgcccac aggcgacgta ttgcgtatcg accgcatcaa ggaaatccac
     5641 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5701 ctgaaagtca tcaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5761 gaaatcgtcg aaggcatcaa acacggcgtg cgcaaagtca acatcgatac cgacctgcgc
     5821 ctcgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga ctttgatccg
     5881 cgcaaatact tgggcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgttatctt
     5941 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaacctg tttcgttgga aaaaatggca
     6001 agccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ctgtaaacaa
     6061 aatgccgtct gaacgccgtt cggacggcat ttgatttttg cttctctgac ctgcctcatt
     6121 gatgcagtat gcaaaaaaag ataccataac caaaacgttt atatattatc tattctgtgt
     6181 atgactagga gtaaacctgt gaaccgaact accttctgct gcctttcttt gaccgccggc
     6241 cctgattctg accgcctgca gcagcggagg ggcggaggcg gtggtgtcgc cgccgacatc
     6301 ggcacggggc ttgccgatgc attaaccgcg ccgctcgacc ataaagacaa aggtttgaaa
     6361 tccctaacat tggaagcctc cattccccaa aacggaacac tgaccctgtc ggcacaaggt
     6421 gcggaaaaaa ctttcaaagc cggcggcaaa gacaacagcc tcaacacggg caaactgaag
     6481 aacgacaaaa tcagccgctt cgacttcgtg caaaaaatcg aagtggacgg acaaaccatc
     6541 acactggcaa gcggcgaatt tcaaatatac aaacaggatc actccgccgt cgttgcccta
     6601 cggattgaaa aaatcaacaa ccccgacaaa atcgacagcc tgataaacca acgctccttc
     6661 cttgtcagcg atttgggcgg agaacatacc gccttcaacc aactgcctga cggcaaagcc
     6721 gagtatcacg gcaaagcatt cagctccgac gatgccggcg gaaaactgac ctataccata
     6781 gatttcgccg ccaaacaggg acacggcaaa atcgaacacc tgaaaacacc cgagcagaat
     6841 gttgagcttg cctccgccga actcaaagca gatgaaaaat cacacgccgt cattttgggc
     6901 gacacgcgct acggcggcga agagaaaggc acttaccgcc tcgccctttt cggcggccgc
     6961 gcccaagaaa tcgctggctc ggcaaccgtg aagatagggg aaaaggttca cgaaatcggc
     7021 atcgccgaca aacagtagta tttgaaaaat gccgtccgaa cacgataatt taccgttcgg
     7081 acggcatttt tgcgccatac cgccctagga cacgacgaca cggcaggcaa gccgatttca
     7141 ctatataatg ccgtctgaac caacgagaac cacaccatgc aagccgattt caaccgcccc
     7201 gtcctcgccg tcgataccgg aacttcctat ttgtcgctcg cgctgcgcgc cgacggcgaa
     7261 atccgtctgt tccatcagga agtcggcatc cgccagtccg aactgatcct gccggaaatc
     7321 cgcaccctat tccgaaatgc gggcattacc gccgccgatt tgggggcgat tgtttatgcg
     7381 aaaggccccg gcgcgtttac cggactgcgt atcggcatcg gcgtggcgca aggtttggca
     7441 acgccgttcg acacgccttt aatcggcatc cccacgctcg atgccgccgc ctcgctgccg
     7501 ccgccgcaaa gctgcatact cgccgctgcg gacgcgcgta tgggcgaagt gttttatgcg
     7561 tggttcgaca cgctgaaccg ccgccgtttg agcgattatc aagtcggtag ggcggcagac
     7621 attgccctcc ccgaggggta cgtcttttca gacggcatcg gcagtgcgtt cgcactggaa
     7681 aaccgtccgc ccttctcagg caaacccgat atgccgaccg ccgccgattt tctcgcgctc
     7741 gccctcggcg gcggctatcc cgcaactggc gcggcacacg ccgaactgct ctacgtccgc
     7801 aacaaaatcg ccctgactgc caaagaacag gcggaacgga aggcacgaac atgaacatcc
     7861 gcccagcctt accgtccgac tgcgccgcac tggccgcgct cgataccgtc tgcaacccgt
     7921 ccgcgtggac gcaacgccaa tttgagtccg cgcttgtttc gccgtccgaa caggttttcc
     7981 ttacggaaaa agacggcagg attgccgcct ttatcgtttg gcagaacctg cccgacgaat
     8041 ccgaactgca cctgattgcc accgcgcccg aatgccgccg acggggcgtt gcgtccgccc
     8101 tgctcgaata ttggtacgca catctgcccg aaggcacgca acgcctgctg ctcgaagtcc
     8161 gcgcaggcaa cgccgccgca caggcactgt ataccaaaca cggcttcagc attgcgggca
     8221 ggcggaaaaa ctattactgc gccgccgacg ggcaaaccga agatgccgtc ttaatggaga
     8281 aaatatgtta agcgcgcgct acctccacct gcacgaggct ttgggcttgg gtccgatgtg
     8341 gctgaaacag gccgccgccg tcctgccgcc caaaaacaca cccgcaaccc cggcacaggc
     8401 acgtccccaa accgtccgcg ccgccacgat ccgcccttcc caaccccata acgttcaggc
     8461 gcggctcgaa acgatgaaag cgttggaaac cgccgccgta catacgcgca aacccgcgcc
     8521 tgaaaccgaa acgcccccgc ccggcctttc agacggcatc gcccccgttc ccgccgcttc
     8581 gggcattacc aagcttgccg tcgtcagcct gtgtccgccg atcgaggatg cggtttacgg
     8641 gcaactgttc cacggcaaag cgggcatcct gctcgacaac atcctcaaag ccgcaggact
     8701 ggatgccgcc tatgtccaca aaacctgttg ggtgaaaacc gccgccgtcg gtaacccgat
     8761 gccgtctgaa caggccgtcg cggatgcgct gggtcaaatc gccggggaac tcgacggctg
     8821 ccgcgccccc gccgtcctat tcctcggaca ggcgtttgtc aacctggaac ggcaggcgat
     8881 gattgaaact ttgtgcggca gccgtccctt cttcatcatc gaccatcccg cccggctgtt
     8941 gcgccaaccc gaactcaaag cccgaaactg gcaggtgttg aaacagttga aacgcgcctt
     9001 gcggcaaggc ggcggcagtt gaagcgcgcc gcacggggcg gtagaatcgc aactgcgtcc
     9061 caatatctga cagaaagcac aaaatgactg atttccgcca agatttcctc aaattctccc
     9121 tcgcccaaaa tgttttgaaa ttcggcgaat ttaccaccaa agccggacgg cggtcgccct
     9181 atttcttcaa tgccggcctc ttcaacgacg gcgcgtccac gctgcaactg gcaaaattct
     9241 atgcacaatc catcattgaa agcggcatcc gattcgatat gctgttcggc cccgcctaca
     9301 aaggcattat tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc
     9361 cgtttgccta caaccgcaaa gaagccaaag accgcggcga aggcggcgtg ttggtcggcg
     9421 cgccgcttaa agggcgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac
     9481 gcgaatcaat caaactgatt gaagcggagg gtgcaacccc cgccggtgtc gccatcgcgc
     9541 tcgaccgcat ggaaaaaggc acgggtaaat tgtccgccgt tcaggaagtg gaaaaacaat
     9601 acggcctgcc cgtcgccccc atcgccagcc tgaacgattt gtttatcctg ttgcaaaaca
     9661 accccgaatt cggacagttc ctcgaacccg tccgcaccta ccgccggcag tacggcgtag
     9721 aataaagcat atgccgtccg aaccacctgc cgcttcagac ggcatcaaac cgacacacac
     9781 cgagaaaaca tcatgcccgc ctgtttctgt ccgcactgca aaacccgcct ctgggtcaaa
     9841 gaaacccagc tcaacgtcgc ccaaggcttc gtcgtctgcc aaaaatgcga agggctgttt
     9901 aaagccaaag accatctggc aagcacgaaa gaacctatat tcaacgattt gcccgaagct
     9961 gtttcggatg tcaaactcgt ccaccgcatc ggcacgcacg ccattagcaa gaaacagatt
    10021 tcccgcgacg aaatcgccga tatcctcaac ggcggtacaa ccctgcacga tacgccgccc
    10081 gcaaccgccg ctgccgcacc tgccgccgca ccgcaggttt ccgtaccgcc cgcccgtcag
    10141 gaagggctca actggactat tgcaaccctg ttcgcactta tcgtcctcat tatgcagctt
    10201 tcctacctct tcatcctatg aacgcgcccg acagctttgt cgcccacttc cgcgaagccg
    10261 ccccctacat ccgccaaatg cgcggcacga cactggtcgc cggcatagac ggccgcctgc
    10321 tcgaaggcgg caccttaaat aagctcgccg ccgacatcgg gctgttgtcg caactgggca
    10381 tccgactcgt cctcatccac ggcgcgtacc acttcctcga ccgcctcgcc gccgcgcaag
    10441 gccgcacgcc gcattattgc cggggtttgc gcgttaccga cgaaacctcg ctcggacagg
    10501 cgcagcagtt tgccggcacc gtccgcagcc gttttgaagc cgcattgtgc ggcagcgttt
    10561 caggattcgc gcgcgcgcct tccgtcccgc tcgtatcggg caacttcctg accgcccgtc
    10621 cgataggcgt gattgacgga accgatatgg aatacgcggg cgttatccgc aaaaccgaca
    10681 ccgccgccct ccgtttccaa ctcgacgcgg gcaatatcgt ctggatgccg ccgctcgggc
    10741 attcctacgg cggcaaaacc ttcaatctcg atatggtgca ggccgccgct tccgtcgccg
    10801 tctcgcttca agccgaaaaa ctcgtttacc tgaccctttc agacggcatt tcccgccccg
    10861 acggcacgct cgccgaaacc ctctcggcac aggaagcgca atcgctggcg gaacacgccg
    10921 ccagcgaaac ccgacgactg atttcgtccg ccgttgccgc gctcgaaggc ggcgtgcatc
    10981 gcgtccaaat cctcaacggg gccgccgacg gcagcctgct gcaagaactc ttcacccgca
    11041 acggcatcgg cacgtccatt gccaaagaag ccttcgtctc catccggcag gcgcacagcg
    11101 gcgacatccc gcacatcgcc gccctcatcc gcccgctgga agaacagggc atcctattgc
    11161 accgcagccg cgaatacctc gaaaaccaca tttccgaatt ttccatcctc gaacacgacg
    11221 gcaacctgta cggctgtgcc gcactcaaaa cctttgccga agccgattgc ggcgaaatcg
    11281 cctgccttgc cgtctcgccg caggcacagg acggcggcta cggcgaacgc ctgcttgccc
    11341 acattatcga taaggcgcgc ggcataggca taagcaggct gttcgcactg tccacaaata
    11401 ccggcgaatg gtttgccgaa cgcggctttc agacggcatc ggaagacgag ctgcccgaaa
    11461 cgcggcgcaa agactaccgc agcaacggac gaaacccgca tattctggtg cgtcgcctgc
    11521 accgctgacc gcaacggaaa gccgccgcag aaatgccgcc tgaaccccgt ttcagacggc
    11581 atttccccga tgacaaagac ctgcccgacc ctcaaggacc ccgatgaaat cctaccccga
    11641 cccctaccgc cattttgaaa acctcgattc cgccgaaacg caaaacttcg ctgctgaagc
    11701 gaatgccgaa acgcgcgcgc gttttttaga aaacgacaag gcgcgcgcac tttcagacgg
    11761 cattttgaat caaatgcagg acacgcggca gattccgttt tgtcaggaac accgcgcgcg
    11821 gatgtaccat ttccatcaga atgcggaata tccgaagggc gtgtaccgca tgtgtacggc
    11881 ggcgacctac cgttccggct atcccgagtg gaaaatcctg ttttcggtgg cggatttcga
    11941 tgagttgctc ggcgacgatg tgtatttggg cggcgtgtcg cacttggtgg agcagcccaa
    12001 ccgcgcgctg ctgactttga acaaatcggg cggcgatacg gcgtatacgc tggaagtgga
    12061 tttggaagca ggggaattgg tagagggcgg ttttcacttt ccggcaggca aaaaccatgt
    12121 gtcgtggcgc gatgaaaaca gcgtgtgggt gtgtccggct tgggacgaac gccagttgac
    12181 cgaatcgggc tatccgcgcg aagtgtggct ggtggaacgc ggcaagagtt tcgaggaaag
    12241 cctgccggcg taccaaatcg ataaaggcgc gatgatggta aacgcgtggc gttacctcga
    12301 tccgcagggt tcgccgattg atttgattga agcgtcggac ggtttttaca ccaagacgta
    12361 tttgcaggtg tcgtccgaag gcggggcgaa accgttgaac ctgcctaatg attgcgatgt
    12421 ggtcggctat ctggcgggac atcttttgct gacgctgcgc aaggactggc accgcgcgaa
    12481 ccaaagctat ccgagtggcg cgttggtggc ggtgaaactg aatcggggcg aactcggggc
    12541 ggcgcagctt ttgtttgcgc ccgatgaaac gcaggcattg gaaagcgtgg aaacgaccaa
    12601 gcgttttgtg gtggcaagcc tgctggagaa tgtacaaggc cgtctgaaag cgtggcggtt
    12661 tgccgacagc aaatggcagg aagccgagtt gccgcacctg ccctcgggcg cgttggaaat
    12721 gaccgaccaa ccgtggggcg gcgacgtggt ttatcttgcc gccagcgatt tcaccacgcc
    12781 gctgacgctg tttgcgctgg atttgaacgt gatggaactg accgtcatgc gcctccagcc
    12841 gcagcagttt gtttcagacg gcatcgaagt gcggcagttt tgggcggtgt cgtccgacgg
    12901 cgaacgcatt ccttatttcc acgtcggcaa aaacgccgcg cccgacacgc cgaccttagt
    12961 ctatgcttac ggaggtttcg gcattcctga attgccgcat tatctgggca gcgtcggcaa
    13021 atattggctg gaagagggca atgcctttgt attggcaaac atccgcggcg gcggagaatt
    13081 cggcccgcgc tggcatcagg cggcgcaggg aatcagcaaa cacaaaagcg ttgatgattt
    13141 gttggcagtc gtgcgtgatt tgtccgaacg cggcatgagt tcgcccaaac acatcggctt
    13201 gcagggcggc agcaacggcg gcctgattac cgccgccgcc ttcgtgcgcg aaccgcaaag
    13261 catcggtgcg ctggtgtgcg aagtaccgct gaccgatatg atccgttatc cgctgctgtc
    13321 cgccggttca agttggacgg acgaatacgg caatccgcag aaatacgaag cctgcaaacg
    13381 ccggctgggc gaattgtcgc cgtatcacaa tctttcagac ggcatcgatt atccgcccgc
    13441 actcattacc accagcctca gcgacgaccg cgtccatccc gcccacgcgc tcaaattcta
    13501 cgccaaactg cgcgaaacct cgccgcaatc ttggctctac tcgcctgacg gcggcggcca
    13561 taccggcaac ggcacccaac gcgaatccgc cgacaaactc gcctgcgtgt tgctgttttt
    13621 gaaagaattt ttgggataag ggcagggggc gacactgccg ccgcgaatga aaaaagatcg
    13681 tctgaaattg ctttttcaga cgatcttttt taatggttgt ttcaaatcaa aatatctatg
    13741 ccgtcggccc atcagcactt tttcacatcc gaaggcaaaa atccgtaatg ccgcctgaac
    13801 gcttcgttga accgtcccgc gtggcggtag ccgcaaaagt gcatcgcggc ttggatggtg
    13861 ctgcccgatt tgatgagggc gagcgcgtgt tccagccgca ggcggcgcag gcgtccggcg
    13921 acggtttcgc cggtttgcgc tttgaaatag cgtttcaggt agcattcgtt cagcccgacg
    13981 cggcgggcga tttcggcgat ggtcagcggg cgggcgaatt cgctgttcaa aatatcggcg
    14041 gcttcgtcta tgcgccggcg gcggtagccg ttgtcgtggc ggcggaaggt gaagcgtaat
    14101 aatcgggcgg agagttccag cgcgtcggct tcgtcggcga gcaggccgaa gccgtccgat
    14161 tcgaacgggc gttgcagcag ggggcaggcc gccgccgtca gtgtccccgc gttttgcgcc
    14221 agccgttgca gggcgaatcg gcctatcgtt tgcggcgaaa acaggcgttc gtccagtaaa
    14281 ccttcgtcgt gccagcggcg cagtttttcc agcgaaaaat ccaaatgcag cgcgcacatg
    14341 ccgccgctgt ctggcagcag ggtttcggat acgtccgcca gttcgccgcg taccagccag
    14401 atttcgccgg cagacgggcg gtattccctg ccgcccattt gtaaccggtt ctgccccgat
    14461 accatgacga acaaggcgca gttgtggctg aaattgtgga tttcggtggg aaacgcaccc
    14521 gttccgccgc cgcgcatccg tgataaggtg atgcccgagt cgaagcggtt gatgcacatt
    14581 ttcagatgca aaccgggctg ttttgcctgc gcaatgagtg cgctgtcgga acagccgtcc
    14641 aacgcccagc cggatttatc ggagcggaca taggtttggt actggcggta gatggcggcg
    14701 gtgttcatga ttggatagga acgagttgtc taacaaatga attaaatagg aattattacc
    14761 aataatcaag cacagggatt ggttggaacg gaaaaggtcg tctgaaaggg tgtttcagac
    14821 aaccttttcc gtttcgggaa tttgttttgc cgtatcggga attttgcgtt ttgcggcgtg
    14881 gtttctgcag gttgtttgct taataataaa cattcttatt cgtatgcaaa ggaaccgcac
    14941 accgtgaaac cgcgttttta ttgggcagcc tgcgccgtcc tgccggccgc ctgttcgccc
    15001 gaacctgccg ccgaaaaaac tgtatccgcc gcatcccaag ccgcatccac acctgtcgcc
    15061 acgctgaccg tgccgaccgc gcggggcgat gccgttgtgc cgaagaatcc cgaacgcgtc
    15121 gccgtgtacg actgggcggc gttggatacg ctgaccgagc cgggcgtgaa tgtgggcgca
    15181 accaccgcgc cggtgcgcgt ggactatttg cagcctgcat ttgacaaggc ggcaacggtg
    15241 gggacgctgt ttgagcccga ttgcgaatcc ctgcaccgcc acaatccgca gtttgtcatt
    15301 accggcgggc cgggtgcgga agcgtatgaa cagttggcga aaaacgcgac caccatagat
    15361 ttgacggtgg acaacggcaa tatccgcacc agcggcgaga agcagatgga gaccctgtcg
    15421 cggattttcg gtaaggaagc gcgcgtggcg gaattgaatg cgcagattga cgcgctgttc
    15481 gcccaaaagc gcgaagccgc caaaggcaaa ggacgcgggc tggtgctgtc ggttacaggc
    15541 aacaaggtgt ccgccttcgg cacgcaatcg cggttggcaa gttggataca cagcgacatc
    15601 ggcctgccgc ccgtggacga atctttacgc aacgaagggc acgggcagcc cgtttccttc
    15661 gaatacatca aagagaaaaa ccccggctgg attttcatca tcgaccgcac cgccgccatc
    15721 gggcaggaag ggccggctgc cgtggaagtg ttggataacg cgctggtatg cggcacgaac
    15781 gcttggaagc gcaagcaaat catcgtcatg cctgccgcga actacattgt cgcgggcggc
    15841 gcgcggcagt tgatacaggc ggcggaacag ttgaaggcgg cgtttgaaaa ggcagaaccc
    15901 gttgcggcgc agtagtttta ttcaaaacaa accacaagga gaaccccatg caaaacgaaa
    15961 ccatcaacct gaaacagcac cttgccgcca tcaaagaata ctggcagccc gaaatcatca
    16021 accgccacgg gttccaattc cgcctggtca aacttttggg cgattacggc tggcacacgc
    16081 acggatacag cgacaaagtg ctgtttgccg tggagggcga catggcggtg gacttcgccg
    16141 acggcggcaa catgacgata cgcgagggcg agatggcggt cgtgccgaag tcggtgtcgc
    16201 accgcccgcg ttcggaaaac ggctgctcgg tggtgctgat tgatctgccc gacccgtccg
    16261 aggccgcctg aaaacgaagt ttccgaagga agctgagttt ctgcgaagct aaaagcagcc
    16321 tacaccttca atatgccaaa agcatag
//
LOCUS       DAXDZG010000054        16200 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXDZG010000054 DAXDZG010000000
VERSION     DAXDZG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.054"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..101
                     /locus_tag="ACFB78_001476"
     CDS             <1..101
                     /locus_tag="ACFB78_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFC1269446.1"
                     /translation="VFACALGALVASEHGATPDISAERIGRFINPV"
     gene            complement(161..718)
                     /locus_tag="ACFB78_001477"
     CDS             complement(161..718)
                     /locus_tag="ACFB78_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1269447.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(793..2427)
                     /locus_tag="ACFB78_001478"
     CDS             complement(793..2427)
                     /locus_tag="ACFB78_001478"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC1269448.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2490..2828)
                     /locus_tag="ACFB78_001479"
     CDS             complement(2490..2828)
                     /locus_tag="ACFB78_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269449.1"
                     /translation="MIFKCRPNAFQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVML
                     FVPDCPVGGAAGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLVF
                     LPCFAPLPYN"
     gene            complement(2825..3931)
                     /gene="serC"
                     /locus_tag="ACFB78_001480"
     CDS             complement(2825..3931)
                     /gene="serC"
                     /locus_tag="ACFB78_001480"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC1269450.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKIPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINDIHPDARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4297..4728
                     /gene="rimP"
                     /locus_tag="ACFB78_001481"
     CDS             4297..4728
                     /gene="rimP"
                     /locus_tag="ACFB78_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC1269451.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4756..6258
                     /gene="nusA"
                     /locus_tag="ACFB78_001482"
     CDS             4756..6258
                     /gene="nusA"
                     /locus_tag="ACFB78_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC1269452.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6270..9101
                     /gene="infB"
                     /locus_tag="ACFB78_001483"
     CDS             6270..9101
                     /gene="infB"
                     /locus_tag="ACFB78_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC1269453.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVGGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9261..10601)
                     /locus_tag="ACFB78_001484"
     CDS             complement(9261..10601)
                     /locus_tag="ACFB78_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC1269454.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10675..12015)
                     /locus_tag="ACFB78_001485"
     CDS             complement(10675..12015)
                     /locus_tag="ACFB78_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC1269455.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12158..12784
                     /locus_tag="ACFB78_001486"
     CDS             12158..12784
                     /locus_tag="ACFB78_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC1269456.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12849..14267)
                     /locus_tag="ACFB78_001487"
     CDS             complement(12849..14267)
                     /locus_tag="ACFB78_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1269457.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     MRKKAGQDLSYQYEFHEFHDKQ"
     gene            14594..15322
                     /locus_tag="ACFB78_001488"
     CDS             14594..15322
                     /locus_tag="ACFB78_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC1269458.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15381..15869)
                     /locus_tag="ACFB78_001489"
     CDS             complement(15381..15869)
                     /locus_tag="ACFB78_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC1269459.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGAAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4245 a         4619 c         4136 g         3200 t
ORIGIN      
        1 aggtctttgc ctgcgcactt ggtgcgctgg tcgcctccga acatggcgca acacccgaca
       61 tttccgccga acgcatcggg cgtttcataa acccggttta aaatgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattttgttt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tcctcgcaac cgaaagcctc ccgcaacaat gggctgttga
      301 cccacatccc cgtaatccag acattgtcga atccctgagc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cacaaacgtt accccgatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca gttcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatgttgc
      601 cgtgatcgga aacttgggtt gccgcctgca atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttt cggacacata tgccgtgaag gcttcagacg
      781 gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt ccttccgata
      841 ggtttggctg ttggaaatgt ccatcagccc caataccgtg ctgaaatagt ggtcgtgcga
      901 atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgcccc cgtgttggcg
      961 gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgtccggaag gcgcgatggc
     1021 gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt ggtcggaaac
     1081 ataatacacc gcgctttcca aatcgtcgcg gttttcaagt ttgcggataa ccttgtcgat
     1141 aaactgatcc acatacaaaa ccgtattgtc gtaagtgttg accaacgtgg cgcgggcgca
     1201 tttgtcgatt tcgttggtat cgcatgtcgg cgtgaatttg cgctcggctt cggtatagcg
     1261 ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg cgtctttatc
     1321 gtttttgttg agggcttcgt cgaacttggt caacaggata ttgtcgaggc actcgccgtt
     1381 gcggcagtat tcgggcaggt tgagcgaggt aacgtcggta ttcggcactt tgccgcacac
     1441 gcccttgcag ccggaatcgt tttccaacca agtaacttcc acgccggcgc gctgtacgat
     1501 gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc ggtcgaaggt
     1561 tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga cctgcgggaa
     1621 attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc tgtaaccgtt
     1681 caaaccccag ttggcggcac gcgtagtctc gcccacgacc agcaccacga aacggcgcag
     1741 gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag gaatattgga
     1801 acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagacg gcacaatcag
     1861 atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt gcaacatcgc
     1921 gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca aaagctcctt
     1981 ataccaaacg cggtatttga ccttgacggc gatatatgcc agcgcgggca atacgcccaa
     2041 acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttccg ccgccgtggt
     2101 ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata tttcttggta
     2161 agacaccgcc gcactgataa ccaatatcaa tggaatcaat actttatgca caaaaggcag
     2221 ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca tcgtatagag
     2281 gaagatgtcc gccccggtgt cgttaaaagg gcgaagttcg acaactttgg caaaaaaggc
     2341 gtaattcaat accagcgaag aatacaggga aagaaaggca atcagcgcgg aagagccgag
     2401 cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt ggaaaaggca
     2461 tccggaatcc ggattattga aaaagattct taattataag gcaacggagc aaagcagggc
     2521 aagaaaacga gtaggcggcg cgggggtttc cgcccgccat tcaaacgtcc ggcagacata
     2581 aaaacatcgt aagcaaaatt cgaaccggtc tgcaaccgcc cctgccggaa aagcgggcaa
     2641 agcatttcat attggaaaaa cccggccgcg ccgccgacgg gacagtccgg cacaaacagc
     2701 atcacgcccg gattgaaaag gacggatagc cgccggcagc acaatcttac cacctccaaa
     2761 acgccgccgg agaacgccga aacagccgat gccgtctgaa acgcattcgg gcggcatttg
     2821 aaaatcaacc gtaacgccgt tggaaatcgc gcataaaatc tgccaaagcc cgcacgcctt
     2881 caagcggcat cgcattataa atgctggcgc gcatcccacc gacagactta tagcccttca
     2941 acaggcacaa gccctgcaat tcggcttcca gcacaaaacg gcggtccaag tcttcgcttg
     3001 cggttttgaa gaccacgttc attttggagc gcgcatcagg atgaatatcg ttgatgtaaa
     3061 aaccgccgct gccatctatc gtctcataca aggtttgcgc cttcagccga ttgaccgctt
     3121 caattttttt cacaccgccc tgcgcctgca gccagcggaa caccagcccc gacatataaa
     3181 tcgcgtaagt tgacggcgtg ttgtacatac cgtcgcggtt caaatgagaa cggtagttga
     3241 acacatcggg aatatcgttc ggacaacgct cgagcaaatc ctcacgcaca atcaccaccg
     3301 taactcctgc cggcccgatg tttttctgtg cgcctgcgta aatcagtccg tagtcggcaa
     3361 catcaaactc acgcgacaga atctcgctgg acatatcgca caccaacggc ggcatgccgt
     3421 ctgaaaggcg cggcacttca cggtattgca gcccgttgac agtttcattg acggcaaaat
     3481 ggacaaacgc cgaatcgggt gcaacatccc acgtttccac aggcggcagg tcgagatagt
     3541 cgaactgctc gcccccgtgc gccgccaaac ggatttccgt atcggtcaaa cggctcatct
     3601 gttcataggc gatacggctc cagttacccg ttaccaccgc gtcggcagtg cggaaaccgt
     3661 gtgccagatt catggctgcc atattaaatt gggttgttgc tccgccctga agaaacaata
     3721 tcttatagtt gtcagggatt ttcaaaagct gcctcaaatc ctgttccgca tgatgcagga
     3781 tgcttaaaaa catttccgaa cggtggctca tttccatcac ggaaaaaccc gtaccgttgt
     3841 agtccgacat ttcctgctgc gccgtcctca acacagcttc aggcaacacg gcagggccgg
     3901 cggaaaaatt gtaaatcgga taaagagaca tgatgcagcc ctggttctga acaataaccc
     3961 gcccgatttt aggcttgcag caggctttgt gcaaggatgg gaaatacctg tcctccgccc
     4021 gattccatgc cgcccgaaca cggaaaataa tatcaatata ttgatttaca aacataaaaa
     4081 tcatgctagc gacaaataga tacatttgtt ttgtcaacaa tattcacgat ttcccattac
     4141 aaacctcccg tacacccgct tttttccgtc ccaaaaacac aaaataaatc aacactttca
     4201 tttctccgca aaagcggtta taatcacgcc gatttttcaa cttttgacga aaaatggcca
     4261 cggggccatt tttttattat gtacataggg agcagcatgg atattcaaac catcctcgaa
     4321 aaaaccctgc ccggcctggg atacgaactg gtcgatttcg aactggccgc gcaaggaaca
     4381 ttgcgcgtgt tcatcgacaa agaaggcggc attaccgtcg aagactgcgc caccgtcagc
     4441 aaccacttga gccgcgtctt catggttgaa gacatcggct acaaaaacct ggaaatttcc
     4501 agccccggac tcgaccgccc cttgaaaaaa gccgccgact tcgtgcgctt tgccggtcag
     4561 aatgccaaaa tcaaaacccg cctgccgata ggcggtcaga aaaactttat cggtaaaatc
     4621 gaaggctgcg aaaacgacac tgttaccgtt tccttcgacg gcaaaaccgt gcaaatcgaa
     4681 ttgggcaaca tcgacaaagc ccgcctgcgc cccgaattca aattctaaaa cacagcaata
     4741 ttggagatgt tcaaaatgag tcgtgaaatg ttacagctgg cagaagcact ggcaagcgaa
     4801 aaaaacgttg atgcggaagt cgtcttccaa gcactggaat tcgcactctc taccgccgcc
     4861 aagaaaaagg cagaccgcga acacatggac gtgcgcgtcc aaatcaaccg cgataccggc
     4921 gaataccaaa ccttccgccg ctggctgatt gtggccgatg aagactatac ctatcccgat
     4981 gtcgaaaaaa ccatcgagga aatccaagag gaaatccccg atacgaccat ccaaatcggc
     5041 gaatactacg aagagcagct gcccaacgaa ggcttcggcc gccaagcggc gcaaactgcc
     5101 aaacaaatca tcctgcaacg catccgcgat gccgagcgcg agcagaatct gaacgagttc
     5161 ctcgccgtca aagaagacat cgtgtccggc acggtcaaac gcgtcgaacg ccacggcatc
     5221 atcgtcgaag tcgttgccgg caaactggac gcgctgattc cgcgcgacca aatgattccg
     5281 cgcgaaaact tccgcagcgg cgaccgcatc cgcgccctct tcctgcgcgt cgaagaaatc
     5341 ggcaacaccg gccgcaaaca agtcatcctg agccgtactt ccggcgattt cctcgtcaaa
     5401 ctgtacgcca atgaagtacc tgaaattgca ggcggcatgc ttgaaatccg cgctgtcgcc
     5461 cgcgacccgg gccagcgtgc caaagtcgcc gtcaaagcca acgaccagcg catcgatccg
     5521 caaggcacct gtatcggcgt tcgcggttcg cgtgtcaatg ccgtcagcaa tgaattgtcc
     5581 ggcgagcgca tcgacgttgt cctttggtcg cccgagcctg cccaattcgt catgagcgcg
     5641 ctctcacccg ccgaagtcag ccgcatcgtc atcgacgaag acaaacacgc cgtcgatgtc
     5701 atcgttgccg aggaccggct cgcgctcgcc atcggacgcg gcggtcaaaa cgtgcgcctt
     5761 gcttccgacc tgaccggctg gcagctcaac atcatgactt ccgccgaagc agacgaacgc
     5821 aatgcggcag aagatgccgc catccgccgc ctgtttatga atcacttgaa tgtagatgaa
     5881 gaaaccgccg acgtactggt tcaagaaggt tttgcaacct tggaagaagt cgcctatgtt
     5941 cctgccgccg aactgcttgc cattgaagga tttgacgaag aaatcgtcga tatgctccgc
     6001 aaccgtgccc gcgatgccat cctgaccatg gcgattgctg ccgaagaaaa actgggcgaa
     6061 gtgtccgacg atatgcgtaa cctcgaaggc gtagatgccg atatgctcct cagccttgcc
     6121 gaagcaggca ttaccacccg cgacgacttg gcagagcttg ccgtggacga actgattgaa
     6181 atcaccggtg taaacgaaga aactgcaaaa gccgtcatcc tgaccgcacg cgaacactgg
     6241 tttaccgaag acaaataaag ggggtacaga tgagtaacac aaccgtagaa caatttgccg
     6301 ccgagctgaa acgccccgtc gaagacctgt tgaaacagtt gaaagaagcc ggcgtcagca
     6361 aaaacagcgg cagcgattcc ctgacgctgg acgacaaaca gcttctgaac gcctacctga
     6421 ccaagaaaaa cggcagcaac ggcggcacca tcagcatccg ccgcaccaaa accgaagtca
     6481 gcaccgttgg cggcgtaaaa gtcgaaacac gcaaacgcgg acgcactgtc aacattcctt
     6541 ctgccgaaga attggcagca caagtaaaag ccgcccaaac ccaagccgca cctgttcagc
     6601 cggagcagac ggcagaagac gcggtaaaag cccgagccga agctgccgca cgcgcagaag
     6661 cccgtgccaa ggcagaagcg gaagcggcaa aactgaaagc ggcaaaagca ggcaacaaag
     6721 ccaaacctgc cgcgcagaaa cctaccgaag caaaagccga aaccgcaccc gttgcggcgg
     6781 aaaccaaacc cgccgagccc aaagaaaaag ccgtcaagcc gaaacacgag cgaaacggca
     6841 aaggcaaaga tgccaaaaaa ccggcgaaac ctgccgcacc tgccgtgccg caacccgtgg
     6901 tcagcgcgga agaacaggcg caacgcgacg aagaggcgcg ccgcgccgcc gcattgcgcg
     6961 cccaccagga agccctgttg aaagagaaac aggaacgcca ggcacgtcgc gaagccatga
     7021 aacaacaggc agaacaacag gcaaaagccg cacaggaagc caaaaccggc agacagcgtc
     7081 ccgccaaacc tgccgagaaa ccgcaggcag ccgcaccggc cgtcgaaaac aaacctgtca
     7141 atccggcaaa agcgaaaaaa gaagaccgcc gcaaccgcga tgacgaaggt caaggccgca
     7201 acgccaaagg caaaggtgca aaaggcgggc gtgaccgcaa caatgcccgc aacggcggcg
     7261 acgagcgcgt acgcggcggc aagaaaggca aaaaactcaa actcgagccg aaccaacacg
     7321 ccttccaagc accgaccgaa cccgtcgtac acgaggtttt ggttcccgaa accattaccg
     7381 ttgccgattt ggcgcacaaa atggcggtca aaggcgtgga agtggtcaaa gccctgatga
     7441 agatgggcat gatggttacc atcaaccaat ccatcgacca agacaccgcc ctgattgtcg
     7501 tcgaagaact cggacacatc ggcaaacccg ccgccgccga cgatcccgaa gccttcttgg
     7561 gcgagggcgc ggaagcggtg gaagccgaag cattgccgcg tccgcccgtc gttaccgtca
     7621 tgggccacgt cgaccacggc aaaacctcgc tgctggacta catccgccgc gccaaagtgg
     7681 tacagggcga agcgggcggc attacgcagc acatcggcgc gtaccacgtc aaaaccccgc
     7741 gcggcgtgat taccttcttg gacaccccgg gccacgaagc ctttaccgct atgcgcgcgc
     7801 gcggcgcgaa agcaaccgat atcgtgattc ttgtggtcgc cgccgacgac ggcgtgatgc
     7861 cgcaaaccat cgaggcaatt gcccacgcta aagcggcagg cgttccgatt gtggttgccg
     7921 tcaataaaat cgataaagac actgccaacc ccgaacgcat ccgtcaggaa ctgacccaac
     7981 acgaagtcat ccccgacgat tggggcggca cggttcaatt catcgacgtt tccgcgaaaa
     8041 aaggaacgaa catcgacgcg ctgctcgaag ccgtattgct cgaagccgaa gtattggaac
     8101 tgaccgcacc tgtcgatgcg cccgccaaag gcatcatcgt cgaggcgcgt ttggacaaag
     8161 gacgcggcgc ggttgccaca ttgctggttc aaaacggtac gctgaaaaaa ggcgacatgc
     8221 tgctggccgg tacggcattc ggcaaaatcc gcgcgatggt cgatgaaaac ggcaaatcca
     8281 ttaccgaagc cggcccgtcc atccccgtcg aaatcctcgg cttgtccgac gtaccgaatg
     8341 cgggtgaaga cgcgatggta ttggcggacg agaaaaaagc gcgcgaaatc gccctcttcc
     8401 gccaaggcaa ataccgcgac gtgcgccttg ccaaacagca ggcggcgaag ctggaaaata
     8461 tgttcaacaa tatgggcgaa acccaggccc aatctttgtc ggtcatcatc aaggcagacg
     8521 ttcagggctc ttacgaggct ttggcgggca gcctgaaaaa actgtccgcc gacgaggtga
     8581 aagtgaacgt gttgcacagc ggcgtgggcg gcattaccga atcggatgtc aaccttgcca
     8641 tcgcttcggg cgcgttcatt atcggcttta acgtgcgtgc agatgcctct tcgcgcaaac
     8701 ttgccgaaaa tgaaaacgtg gaaatccgct actacaacat catctacgat gccatcgacg
     8761 acgtgaaggc ggcgatgagc ggcatgcttt ctcccgaaaa gaaagaacag gttaccggta
     8821 cggtcgaaat ccgtcaggtc atctccgttt ccaaggtcgg caacattgca ggctgtatgg
     8881 tcaccgacgg cgtggtcaaa cgcgattccc atatccgcct catccgcaac aacgtggtca
     8941 tccacacggg cgaactggct tcgttgaaac gctataaaga cgacgtaaaa gaagtccgca
     9001 tgggcttcga gtgcggtctg atgctcaaag gctacaacga aatcatggaa ggcgaccaac
     9061 tggaatgctt cgacatcgtc gaagttgccc gcaccctgta attcctttgc aaataaaatg
     9121 ccgtctgaag cgttcagacg gcaacgaaac gggttctgta tcatacagaa cccgtttttt
     9181 gtcgcaaatc ggcttcagcg accctcctgc cttatcccga tttgaatctg acttgccata
     9241 caaacaggct tcagacggca ttatttgccc gccaaacgta tcccgatctt ctccgcatat
     9301 tccctgcgtt cggcgcggct ggtttccggg cggtgcgtat tgagcgacga ccatttccaa
     9361 tgactgcggg ctttgttgag ttcgggcggg agtttggcgg catcccacgg ggctttgcgg
     9421 ctgtgcagct cgatatccga ctgtgccgcg tgtccgcgcg tttgcaggac gtggagcaga
     9481 tcgagggcgc gggcggcgag cagggtcagg gtttcagggt cggtgtgcag ggtttgacgg
     9541 ccggcgagtt tgtcggaaat ggtgcgggta ttgggcagga tgccgcccaa aaagccgccg
     9601 attgccgtac ccaatccgag cgagccgccg agtgtggcga tgtccagccc caagccgatg
     9661 agcgcgccgg ttgccgcgcc cgtgccggtg cggatgccgt attgtttgag caattcgctg
     9721 tcgaacgggt cttggcggaa ggcttgcggc atccagtcgc cgccgtcgat ttcgctgtgg
     9781 tagaaacggt aaagggcaaa cagccgctgc tgcatctgcc gttcgagttg gcgtatttcc
     9841 gcctgcatgg tttgcagcac ggtggcggta tcctcgtttt cgtccacttc ctgcctgaag
     9901 gcggcggcat cgagcaggaa atcggcgatt tcgcggcgcg cttcgccgtc cagccgctgc
     9961 cattcgcgcc ggcgcatggc tgtcaggcgg tcaagtgtgc tgcgttcggg caacatggtg
    10021 gcgaggtttt cccacaggcg caattcgcct tcaaaatcaa aggcgacggt gtcgaaccct
    10081 gcgaaaacgt gcaggtttct cctcgccagc atggttgtcc acgattcggg aagctgtccg
    10141 ccggtaaagt tgaacacggg cataaccggt ttggcacacc atgaaaggat ggtcaattca
    10201 tcccgatatt tgtcgaggac gggttcgcgc gcgtcgatga cgtacattgc catatcgctt
    10261 tgcaaaacct gccgcagcac tttggcttct tggttgaaat cgtgatgcgc gccgtggctg
    10321 ccgagaaact gttgcagccg ctcgatgccg tctgaacgat tgtccgtatg gttttccagc
    10381 cattccaaaa caccgcccgc gtcttcgagt ccgggcgtgt catacaggaa aaccaacatg
    10441 tccgcgccgt cgctgatggc ggcttcttcg acatggcgtg tggtggacgg tgcgtttctg
    10501 acttcgccga agccgctgtc gcgcaacagg gtgcgcagga gcgaggtttt gccggtgttg
    10561 gtgtgtccca cgacggcgag ggaaaggggt tgtttgttca tgatgttttt gaagaatgga
    10621 ttttcagacg gtcttttttc agaatggcgg cttaacagaa catttcaagt gagtttattg
    10681 gtctttcaaa cggccttcct gcgccaccct gtcaggctca agccacgccg cgccgcattc
    10741 ggtcagcgcg ttacgccaat gttccagctt ttccgaaagg tcgtctgaaa gcccctgttc
    10801 cgccaaaagc tgcaccaccg cgccgccctg cgccgcttcc gaaagccgca caatctgccg
    10861 cagcacgccc cggtccggca cagtttgggc gcgtacgccg ataagcagtt gcgccggttt
    10921 ctgcttcagc tctgtctcca gcgcggcaac ctgttcccga ttggcggcaa cgcccttatc
    10981 cagccattcc tgcgccagcc tgccctcgaa ccattggccg tcctgccact cggtctccag
    11041 catgagcgcc catttcggcg catcgttcaa gacgattttc ggcgaaacgg cggacacggt
    11101 ttcccgacgc gtatccgcat cggtgatttt gttctgccag cggcggatga ccgcctgata
    11161 ataggttttt tccaaatcca atccgttttc gcttgttttc aaaaggattt tacacactac
    11221 ccaagccaag aggcgcggca ggatgccgta gcagacgata ctgccgacca gcagccccga
    11281 ccaagcccgc gcatcggcaa tattgccgtt cagacgacct tcgatgaccg cccgcgcatc
    11341 ggggacaggg aaaccgagtt tcgacggcag ccatgccaac atttccaccg cgcgtaccga
    11401 agcggcattg ctcaacagcg tgctttccca gttgaacgta tattgccgca ccaaaagcag
    11461 cagcaatacc gacaccagca ttccgagcag cgtgcagagc cacaagctgt gcgccgttgc
    11521 gcctattttc catcgtaccg aaggttgccg ccactggtcc gcatacagcc gcaacaccgc
    11581 ctgatttaca gggcctttgc cccgaaacca cgtcgccgga ctgctgaaaa accgccccac
    11641 tttcacgcgc aggaacaacg ttgccaacca tactgccagc atcagcgtat tcatgcccaa
    11701 cactcccgcc aaaactaaaa agaaattcag cccctgattg tccatcagaa gataagtgcc
    11761 tgaaaatccg gcggtaaaca tcatcgatgc cgccaccacc cataaccaga acgaccccgc
    11821 acgcacacgt tccaacgtgt cccgcagcat acggtccctg tcgatcatct ccgcccgacg
    11881 gatgattttt tcctccgtac tgccgtccac gcggcgcaaa gcctccgtcg cctgcacggg
    11941 atcgccgctg aaaataaaac cgccttcatc caaaatacgg accagctcaa ccagttttcg
    12001 ggatggattc aacataaaat gccgtctgaa aataaaaaac aaattttaac acacgcattt
    12061 tcaagaatat tcacagtgta ggcaaagagt aaatcccaca cagaggcaaa agtatcggcg
    12121 taaactgact gcctctactt tcccgaaaga ttgtgcgatg tatgcaggcg aacgcttcaa
    12181 tacttacagc catttgagcg gtttgattct ggcggcggca ggtttgatgc tgatgctgct
    12241 gaaaaccata ggacacgggg acggctaccg tatcttcagc gtatcggttt acggcatcag
    12301 ccttcttctg ctctatttga gttcctcgct gtaccacgga attgcagccg gaaaactgaa
    12361 aagcattttg aaaaaaaccg accactgcat gatttatgtg ctgattgccg gaagctacac
    12421 accgtttgca ctggtttctt tgagaaacgg gccgggctgg acggtatttt cactgtcctg
    12481 gctgctggcg gctgcaggaa tcgcacaaga actcaccatc ggacggaaaa gcgaaaaacg
    12541 tctgctgtct attgcgattt atatcgtaat gggctggatg gtcttggcgg taatgaaatc
    12601 cctgacagcc tcactcccgc cggcaggact ggcttggctg gcggcaggcg gtatgctgta
    12661 cagcgtcggc atttactggt ttgtaaacga tgaaaaaatc cgacacgggc acggaatctg
    12721 gcatctgttc gtattgggcg gcagcataac ccaatttgtc agcgtgtacg gttatgtaat
    12781 ctgaatgccg tctgaaacac aaaaacctcc caaacctgaa ggcagggagg ttttctcttt
    12841 gccgggcatt attgtttatc gtggaactcg tggaattcat actgatagga taaatcctgt
    12901 cccgcctttt tccgcatcag gtagtcatca taaatggcgc ggatttcctt acgcagcaag
    12961 aataaggcaa tcaggttggg gataaccata aagccgttga acatatcgga cagattccaa
    13021 accaaatcga ctttgccgag cgtacccaaa acgatagcaa gcagaaccaa tgcgcgatag
    13081 atgcccaagt gtcttcccct gaaaagaaaa cggatgttgg actcgccgaa ataataccag
    13141 ccgataatgg tcgtaaaggc gaagaaggtc agacacacgg caagcaattg cgaaccgaag
    13201 cccggaaatg ccttgttaaa ggcaaattga gtaaccgccg cgccctgttc gcccgaaagg
    13261 ttggcatcgg tcagcaggat aatcaatgcc gtagccgtac ataccaaaat cgtatcgata
    13321 aatacaccga caaatgccgt cataccttgc tgaacaggat gcttcacatc cgcagtcgcg
    13381 tgggcgtgcg gagtcgaacc catacctgct tcgttggaaa acagaccgcg cgccacgccg
    13441 aaacgtatcg cttcacgcat accgataccc gcagcaccgc ccaaaacggc ctcgggattg
    13501 aaggcggcgg taaagatgtg gttgaacatc ggcacaatat ggtcggaaaa ttcaaacagg
    13561 ataacgacgg cgcacaaaat ataaacaacc gccataaacg gcacgacaaa ccgggcaata
    13621 ttggcaatac ggttcacgcc gccaatcaca accatgcccg caaggacggc aaacacaata
    13681 ccgactgcca aagaaggcac atcaaatgca atggtaacgg cagaagcgat ggaatttgcc
    13741 tgagtcgcat tgccgataaa gcccaacgcg acaatcagcg cgatggagaa aaagcccgac
    13801 aggaaacgcg ccgcgcccct gccgattttc ggagtcagac cgtgcgtaat ataaaacgcc
    13861 ggcccgccga tgtatttgcc gtggctgacg acgcggtatt tctgcgccag cagtgcttcc
    13921 gcaaaaatcg tggacatccc caaaacggca gaaagccaca tccaaaaaat cgcacccggc
    13981 ccgcctgcgg taatggcggt cgccacaccg gcgacgttgc ccgtaccgat ttgcgcggat
    14041 atagcaaccg ccaacgcctg aaactgcgat aaggacttat cgtctttatc acctttggca
    14101 aacaagccgc cgaatacgga tttgaatccc gcgcccagct tggtaatctg cggcgcacca
    14161 agatacagcg taaaaaacag gccgataccc aaaagcgcat aaatcagcag gtagccccaa
    14221 agaaaccgat tgactgtacc caccagaaca gacaatatat tttccataaa ataaacctta
    14281 tcttacgatt aaaatgactg ttttccaaaa gacattccaa taaggaaaca cggcgagcag
    14341 accgtatttg ccgcaacaga tgccttaaat tgtcaataat ccgagagaag ctgtgccagc
    14401 ataccgtaaa atatcgttaa ttaaaatatt tctttatttt taagcggaaa gcggaggaaa
    14461 tcgttttcag acggtatcga caacggcacg gcataaaaca ggatattttg ggtacttgca
    14521 acttatgtta aaatgccgac cgtaaaaaat ctgacaaaaa cagattaatt atttgaaata
    14581 agaaaggaaa tttatggcag gccatagcaa gtgggcgaat atccagcata aaaaagcccg
    14641 tcaggatgcc aaacgcggca aaatcttcac ccgtttaatc aaagaaatca ccgttgcggc
    14701 gcgtatgggc ggcggcgatc ccggcgcaaa tccgcgcctg cgtctggctt tggaaaaagc
    14761 agccgaaaac aatatgccca aagacaatgt gcaacgcgcc atcgacaaag gtacgggtaa
    14821 cttggaaggc gtggaataca tcgagttgcg ctacgaaggc tacggcatcg gcggcgcagc
    14881 tttgatggtg gactgcctga ccgacaacaa aacccgcacc gttgcggacg tacgccacgc
    14941 atttaccaaa aacggcggca acttgggtac cgacggctgc gtggcgttca acttcgtgca
    15001 tcagggctat ttggtattcg aacccggcgt tgacgaagac gagctgatgg aagcggcttt
    15061 ggaagccggt gcggaagacg tggttaccaa cgacgacggt tccatcgaag tcattaccgc
    15121 gccaaatgat tgggcgggcg taaaatccgc tttggaggcg gcaggttaca aatccgttga
    15181 cggcgacgtt acgatgcgcg cccaaaacga aaccgaactc tccggcgacg atgccgtcaa
    15241 aatgcaaaaa ctgattgacg cgctggaaga cttggacgac gtgcaagacg tttacacttc
    15301 cgccgtattg aatctggact gatacgcagc acagcagaca tacaacaaaa tgccgtctga
    15361 acctttcaga cggcattgat ttatttagcc cttgcccacg cccaccaaac cgtcaggaca
    15421 accgccaaaa aatacgccgc gctccaaaca ggcaaagcaa cccctaacag ataatccggt
    15481 tcggcacaat ttccgaaccc gcgcacaaca ggctcgaacc aatcaaacaa aggccagccc
    15541 ttcaatcgaa acgtccacgg cgcgccgcac gaaggagccg cacccggcgg cagcgactgc
    15601 aaccacaact gatatgccgc aacagaaata cccgtaacgg cgggaatact gataaacact
    15661 gcgcctgaca gtccgcccac cctgcccttg ggcttacacg ccagcacaac tgccgcacac
    15721 aatgcggttg ccaaaacaca taaccgctga ctgatgcaca aaacgcaagg ctccataccc
    15781 aaaacatact gtgccgccaa agaaccggca aatgcacaga ccgaaacggc aaacagcagc
    15841 caaacggctt ttctaaataa cggggtcatt ttctcaacac accaatcaaa ataccgatat
    15901 gccgattttg ctggatatat cccgagatgg caagggacga atcgacaccg gcacaggtgc
    15961 ggacgatttc ggttttcccg atatgcacaa aacctgacaa aggacaaaac ggcgatttgt
    16021 ccgcacaatg cccgaaaaaa tacatccgga ggatttgaat ttgaatttcg gcaaatttag
    16081 cgaatcaaaa gcaacagccg ataaaaaaat caaaagttta tttcaatgtt tcaacacaca
    16141 ggacgacaca taaagcaccg ccctatgtgc cgtcctgatt tggaaggggt tacacccctc
//
LOCUS       DAXDZG010000055        15958 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXDZG010000055 DAXDZG010000000
VERSION     DAXDZG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15958
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.055"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..258
                     /locus_tag="ACFB78_001490"
     CDS             <1..258
                     /locus_tag="ACFB78_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269460.1"
                     /translation="TNHILKQIISKSDFFKIKFQKKETGKIRHKTLTYSKPSVLQRKY
                     PKKTICLPSVSNRYTAGTRYFSDGAAVVRTDKNVTAAERIA"
     gene            312..2696
                     /gene="ppsA"
                     /locus_tag="ACFB78_001491"
     CDS             312..2696
                     /gene="ppsA"
                     /locus_tag="ACFB78_001491"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC1269461.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWDKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKASKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDTSEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2917..4176
                     /gene="rho"
                     /locus_tag="ACFB78_001492"
     CDS             2917..4176
                     /gene="rho"
                     /locus_tag="ACFB78_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC1269462.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4796..6100
                     /locus_tag="ACFB78_001493"
     CDS             4796..6100
                     /locus_tag="ACFB78_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC1269463.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6194..7489)
                     /locus_tag="ACFB78_001494"
     CDS             complement(6194..7489)
                     /locus_tag="ACFB78_001494"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC1269464.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLCQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7616..8503)
                     /locus_tag="ACFB78_001495"
     CDS             complement(7616..8503)
                     /locus_tag="ACFB78_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC1269465.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8503..9468)
                     /locus_tag="ACFB78_001496"
     CDS             complement(8503..9468)
                     /locus_tag="ACFB78_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC1269466.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9484..10608)
                     /gene="potA"
                     /locus_tag="ACFB78_001497"
     CDS             complement(9484..10608)
                     /gene="potA"
                     /locus_tag="ACFB78_001497"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC1269467.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10886..11155)
                     /gene="rpsO"
                     /locus_tag="ACFB78_001498"
     CDS             complement(10886..11155)
                     /gene="rpsO"
                     /locus_tag="ACFB78_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC1269468.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11236..11370)
                     /locus_tag="ACFB78_001499"
     CDS             complement(11236..11370)
                     /locus_tag="ACFB78_001499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269469.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(11360..12295)
                     /gene="secF"
                     /locus_tag="ACFB78_001500"
     CDS             complement(11360..12295)
                     /gene="secF"
                     /locus_tag="ACFB78_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC1269470.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12299..14152)
                     /gene="secD"
                     /locus_tag="ACFB78_001501"
     CDS             complement(12299..14152)
                     /gene="secD"
                     /locus_tag="ACFB78_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC1269471.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14364..14630)
                     /gene="yajC"
                     /locus_tag="ACFB78_001502"
     CDS             complement(14364..14630)
                     /gene="yajC"
                     /locus_tag="ACFB78_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC1269472.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14761..15879
                     /locus_tag="ACFB78_001503"
     CDS             14761..15879
                     /locus_tag="ACFB78_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC1269473.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPEHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4014 a         4357 c         3993 g         3594 t
ORIGIN      
        1 acaaatcata ttttaaaaca aattatctca aaatcagatt tctttaaaat aaaattccaa
       61 aaaaaagaaa caggaaaaat acggcataaa acattaacct attcaaaacc atctgtttta
      121 caaaggaaat acccgaaaaa aacaatatgc ctgccatcgg tttcaaaccg ctacacagcc
      181 ggaacccgct atttttcaga cggcgcagcg gttgtccgga cagataaaaa cgtgacagcg
      241 gcggaacgaa tcgcttaaaa taagcaagcg gatttgtttc ttttttaaca tattttggat
      301 tggacacaca aatggccgac aactacgtaa tctggtttga aaacctgcgt atgacagatg
      361 ttgaacgcgt gggcggtaaa aacgcctcgc tgggcgaaat gatcagtcaa ctgaccgaaa
      421 aaggcgttcg cgtccccggt ggctttgcca ccacagccga tgcctaccgc gcatttctcg
      481 cacacaacgg tctgaacgaa cgcatttccg ccgctctggc aaaattggat gtcgaagacg
      541 tttccgaact ggcacgcgtc ggcaaagaaa tccgccaatg gattttggac acgcccttcc
      601 ccgaacagct cgatgccgaa atcgaagcgg catgggacaa aatggttacc gatgccgacg
      661 gcgcggacat ttccgttgcc gtacgctctt ccgcaaccgc cgaagacctg cccgacgcat
      721 catttgccgg ccaacaggaa accttcctga atatcaatgg cttggataac gttaaaaaag
      781 cgatgcgcca cgttttcgcc tccctgtaca acgaccgcgc catttcctac cgcgtccaca
      841 aaggcttcga acacgacatc gtcgcccttt ccgccggcgt tcaacgcatg gtgcgttccg
      901 acagcggcgc atcaggtgtg atgttcaccc tcgacaccga atccggctac aaccaagtcg
      961 tctttgttac ctcctcttac ggtctgggcg aaaacgtcgt acaaggcgcg gtcaacccgg
     1021 acgaatttta tgtgttcaaa cccacgctca aagcaagcaa acccgccatc ctgcgcaaga
     1081 ccatgggttc gaaacacatc aaaatgattt tcaccgacaa agccgaagcc ggcaaatcgg
     1141 taaccaacgt cgaagtcccc gaagaagacc gcaaccgctt ctccattacc gacgaagaaa
     1201 tcaccgagtt ggcacactac gcgctgacca tcgaaaaaca ctacggccgc ccgatggata
     1261 tcgaatgggg acgcgacggc ctggacggca aactctacat cctgcaagcc cgccccgaaa
     1321 ccgtcaaatc ccaagaagaa ggcagccgca acctgcgccg ctacgccatc aacggcgaaa
     1381 aaaccgtctt gtgcgaaggt cgtgccatcg gtcaaaaagt cggtcagggc aaggtgcgcc
     1441 tgattaaaga tacttccgag atggattccg tcgaagccgg cgacgtactg gttaccgaca
     1501 tgaccgatcc ggattgggaa cccgtgatga aacgtgcttc cgccatcgtg accaaccgcg
     1561 gcggacgcac ctgccacgcc gccatcatcg cgcgcgaact gggtattcct gccgtcgtcg
     1621 gctgcggcaa tgcgaccgaa ctcttggaaa acggtcagga agttaccgta tcctgtgccg
     1681 aaggcgatac cggctttatc tatgccggcc tgctggacgt acaaattacc gatgtcgcct
     1741 tggacaatat gccaaaagca cccgtcaaag tcatgatgaa cgtcggcaat cccgaactcg
     1801 cattcagctt cgccaatctg cccagcgaag gcatcggctt ggcgcgtatg gaatttatca
     1861 tcaaccgcca aatcggcatc cacccgaaag ccttgttgga atttgacaaa caagacgacg
     1921 aactgaaagc ggaaattatc cgccgcatcg ccggttacgc atcccctgtc gacttctacg
     1981 tcgataaaat cgccgaaggc gtggcaacgc ttgccgcatc ggtttatccg cgtaaaacca
     2041 tcgtccgtat gtccgacttc aaatccaacg aatacgccaa cctggtcggc ggcagcgtat
     2101 acgaaccgca tgaagaaaac ccgatgttgg gcttccgtgg tgcggcgcgt tatgttgccg
     2161 agagcttcaa agactgtttc gccttggaat gcaaagcctt gaaacgcgtt cgtgacgaaa
     2221 tgggtctgac caacgtcgaa atcatgattc cgttcgtccg cactttgggc gaagccgaag
     2281 cagtggttaa ggcattgaaa gaaaacggct tggaacgcgg caaaaacggc ctgcgcctga
     2341 ttatgatgtg cgagctgccg agcaacgcgg tattggcgga acaattcctg caatacttcg
     2401 acggcttctc catcggctcg aacgacatga cccagctgac cctcggcctc gaccgagaca
     2461 gcggcttggt gtccgaatcg tttgacgaac gcaaccctgc cgtcaaagtg atgctgcatc
     2521 ttgccatctc cgcctgccgc aagcagaaca aatatgtcgg tatctgcggt caaggtccgt
     2581 ccgaccatcc ggactttgcc aaatggctgg ttggagaagg catcgaaagc gtgtctctga
     2641 acccggatac cgtcatcgaa acttggctgt atttggcgaa tgaattgaac aaataatcaa
     2701 ttcccatacc cccaagcctg aaaagcgcgg gggtattttt tccaagcagc tccgttcaga
     2761 cggcatttcc tgtcgatgcc ccgtccgcga taatatttga cacccacgcg ccgactgcct
     2821 acaattcccc cctctccgag caaccggcaa cagtcagctt cttctttcag acggcatcca
     2881 cccgtctttt cctttctatt ctatatatca ttgattatgc acgtctccga attacaaacc
     2941 ctgcacattt ccaaactctt agaattggcg gaagaacacg gcatcgaaaa cgccaaccga
     3001 ttccgcaaac aagacctcgt atttgccatc gtccgccaga tgatgaaaaa aggcgagggt
     3061 tttacctgct ccggcacgct cgaaatcctg cccgacggct tcggcttcct ccgcagcgcg
     3121 gacacgtcct atcttgccgg ccccgacgac atctatgtct cgcccactca aatccgccgc
     3181 ttcaacctgc atacgggcga caccatcgaa ggcagcgtgc gcgtccccaa agacaacgaa
     3241 cgctattttg ccttggtcag actggattcc atcaatggcg accacccgga agtatgccgc
     3301 cataaaatcc tgtttgaaaa cctgaccccg ctgtttccga ccgaacagtt gaagctggaa
     3361 cgcgacttaa agtccgaaga aaacctgacc ggacgcgcca tcgacctgat ttcgcccatc
     3421 ggcaaaggtc agcgcgccct cttggttgcc ccgcccaaaa tcggtaaaac cgtgatgctg
     3481 caaaacattg cccacgccgt taccgcaaac tatcctgaag tcgaactcat cgtcctcttg
     3541 attgacgaac gccccgaaga agtaaccgaa atgagccgtt ccgtccgagg tgaagtagtc
     3601 tcctccacct ttgacgaacc ggcgcaacgc cacgtccaag ttgccgaaat ggtgcttgaa
     3661 aaagccaagc gtatggtgga acacaaaaaa gacgtggtca tcctgctgga ttcgattacc
     3721 cgccttgccc gcgcctacaa taccgtcgtc cccgcctcgg gcaaaatcct gaccggcggt
     3781 gtcgatgcca acgcgctgca ccgccccaaa cgtttcttcg gtgcggcgcg caacgtggaa
     3841 gaaggcggtt cgctgaccat tatcgccacc gcattggttg aaaccggcag ccgtatggac
     3901 gatgtgattt acgaagaatt caaaggtacc ggcaatatgg aattgcacct cgaccgccgt
     3961 atggcggaaa aacgcctctt ccccgccatc aacatcaaca aatccggcac gcgccgcgaa
     4021 gagctgcttg tgccgaacga ccagttgcaa cgtatgtggt tactgcgcaa gttcctgcac
     4081 ccgatggacg aaatcgaagc taccgagttc ttaaacggaa aaatcaaagc atctaaaaat
     4141 aatgatgatt tctttgaact gatgcgcgga aaataaacgc gccgcccgca ttaatgcgaa
     4201 atgccgtctg aagcctgaaa atcgggtttc agacgacatt ttcattcgca cggccggcgc
     4261 agctcccccc ccccccccgc taacggcgca accgccggca gtgtccgtgt ccgcctgccg
     4321 aaggcgcggc ctttgcaaaa ccggctcgaa cgcatcccta ccggcaatga aaaccgatgc
     4381 gggaacttga ttcaaggttg cgccgaaccg cgcccatttt tgtataaatt ttattttcta
     4441 tttccaatta gaaattttta atatattaat caataaatta attttataaa ataaaaatat
     4501 tgtcaacaat cttttgactt atcgcccaaa cctctgtata ttttcctaca gtaaattgtt
     4561 gacaatccat acgcccgcat atgcgccgcc taaggataaa tcctcccgcc ggacaacggg
     4621 ggcaagggat gggatgcgat atttccatat tcaaacaagg gatttgtttc acgcacaggg
     4681 cggcacatcg gcaaaatccc cgcgccggtc cggcagggct tgcgcccatc ccggacaagc
     4741 cccgaccccg cctttccgaa agacgggccc aaccattaag gaaactttaa tcaaaatgaa
     4801 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
     4861 gtggaaacct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
     4921 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
     4981 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
     5041 ttgggtcgcc gtcggctatt ctttggcgtt cacgccggga aatgccttta tcggcggttt
     5101 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacggatgc tgaccgtgtc
     5161 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
     5221 tatttcgact gccattatta ccggcgcgtt tgccgaacga atgaaatatt cggcaatgat
     5281 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
     5341 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
     5401 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     5461 gcgcgaggcg atgcctccgc acaatatggc gatgacgctg attggcgcgg caatgttgtg
     5521 gttcggctgg ttcggcttta acgctggttc ggcgcttgcg gcagacgcgg cggcggggat
     5581 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     5641 gaaaatagcg ggacacaaac cttctgcttt ggggctggct tccggcgcag tttccgggct
     5701 ggtcggcatc acccctgccg ccggctttac cggtccgtcg ggcgcggccg ccatcggtat
     5761 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     5821 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtaggcg gaatattgac
     5881 cggcatcttt ttcgacaacc gcattttcgg tggggatgcg gcagtttggc agcagttgtg
     5941 gatacaggta aaagacgggt tcattatggc ggcatacagc gggctgatga gttgggcgat
     6001 tttgaaggct gtggggaaaa tctgcggcgg tctgcgcgtc ggcaaggatg tcgaacgcga
     6061 aggtttggat ctgaatatcc acggcgaaca cgtggaataa ggacggctat gccgtctgaa
     6121 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatggg gataaacata
     6181 ctgcctgcga cgcttaacgg ctgtctttcc actgataata tttcgagccg aggaaagaac
     6241 cgagtttgcg caaaaacggt tgcaggataa tattcggctg gcacaactgt tcaaacggtt
     6301 tcagacggca ttcgtcccct aaaattgctt cggcaaccgc caggcctgca atgcccgtta
     6361 tcgccatccc gtgcccggaa taaccttgcg tataaaaaac attcgaggtc aaacgtccga
     6421 aatgcgggac aaggttggcg gtaatatcgc actccccgcc ccacgaatat tcgattctaa
     6481 catcggcaag ctgcggaaaa actttaagca tatcttggcg gacaagctcg gtcatacgcg
     6541 caggattgtc gataaattcg ttatccttac cgccgaaaag cagcctgccg tccgcgctga
     6601 ggcggtaata atccaaaata tggcggttgt cgcatactgc catattgttg cggataagcc
     6661 cttttgtgcg cgcgcccaag ggttcggtgg caataataaa ggtgctgacg gcaatcgcct
     6721 tgcgttccaa aggccggaat atcgggttca aaccgacata agtattgacg gcatagacca
     6781 catttttaca ctcgacgctg ccttcgggcg tgtaaaccag ccaaccgttt tgatacggtt
     6841 cgatgcgcgt catcggggat tgctcgaaaa tctgcgcgcc ggcttcggca gcggcgctgg
     6901 caacacccaa cgtgtaattg agcggatgaa gatgcccgga caagggatcg aactgtgcgc
     6961 cttggtacat atcgctgtca agctgctgtt tcaactcggc tttatcccaa agttgataat
     7021 gactcgcacc gtaatgccgt tgggcgtgtt catgccactg ctgcaactct tcccaatgct
     7081 gcggacggac ggcaaccgtg gcataaccgc gctgccaatc gcaatcgatg gcatgtttgc
     7141 ggacgcgttc gtccaccagt tcgaccgcct gcaaagactg ttgccaaaac cattgcgcct
     7201 gctccaagcc gacctgtttt tcaatttccc ccataccgca ggcgtagtcg ctgataacct
     7261 gcccgccact cctgccggac gcgccgaagc cgatacgtgc ggcttccaaa acgacggctt
     7321 catgtccgtg ttccgccagc ggcaatgcgg tacacaaacc gctcaaaccg ccgccgataa
     7381 tgcaggtttc ggctttcaga cggcattgga gtttcggata aacagtatgc ggattaaccg
     7441 aactaaaata ataagaaggc agatattctt gaaaatcagg gcgaatcatt gtgtttgctt
     7501 tatcgggtat attttcggac ggaatgatac aggctgtcgg gccatatcgt ccaaacagaa
     7561 aatcggttga aaaaaaacag gctgacccag tcatgcggtc agcctgcctt attaattaat
     7621 ttgctttctc ggcagccaat ttttcttggc ggtaggcttc tgccgcttct cggttgcgct
     7681 tggttgcctg cctcatcatc caataattga cgatgatgac caatgttccg atgatgccga
     7741 tcaggatggt tgccaagaca ttcatctgag gatcgagacc caacttgatc ttggagaaaa
     7801 taacctgcgg caatgtggat gaaccggggc cggagaggaa tgaggtaatt accaaatcat
     7861 ccaaagacaa ggtaatgccg agtaggaagc ctgaagcaat ggcaggggca atcaaaggca
     7921 aagtgatgac aaaaaagatt ttcagcggac gcgcgcccaa gtccattgcg gcttcttcga
     7981 gcgactggtc aagctcgacc agccgcgaac ggatgacaac ggtaatgtat gccatgcaca
     8041 gcgtcgtatg tccgaggaag atggtgaaaa agccgcgatc gaagtagaga tgttgcaacc
     8101 attcgctgcc ctgcaaaaat atctgcacct gaataatcag cagcagcata gacagaccgg
     8161 taatcacgtc gggcatgacc atgggcgcgg aaatcatacc ggcaaacagc gtgctgccgc
     8221 gaaagcgttt gatacgcgcc atcgcatagc ccgccagcgt gcccaaaacg acggcggcaa
     8281 gcgaagacac aacggcaatc cgcagcgaca gccaagcggc ttccaaaatg gtgtcgtttt
     8341 ccagcaatgc gccgtaccac ttggtcgaaa agccgcccca aacggtaaca agtttggatt
     8401 cgttgaacga atagatgacc aaaacaacca gcgggatata cagaaacgcc agcgatagtg
     8461 ccaacatcag tttcaagaac caagataatt tggatttctg cattatttgg ctccttcttc
     8521 caattcgcgg ttttcatagt gctggaacag ggcaatcggt acaaccagta gcgcgaccat
     8581 cacgacggcg acggcagaag ccaacggcca gttgttttga tcaaagaatg cctgccacaa
     8641 tactttaccg atcatcaggt tttccgaacc gccgaccagc tcgggaatga cgaactcacc
     8701 gacagcgggg acgaaaacca gcatcgaacc tgcaataatg ccggttttcg acaaaggcag
     8761 ggtaatcgtc aagaacgatt tgaccggtcc cgcgcccaag tcggaagccg cttcgagcag
     8821 gcggctgtcc agtttcacca gctgcgtgta aagcggcaga atcataaacg gcagataggc
     8881 ataaaccatt accaaattta acgaaaacgc attatagaac aaatctaatg gttctctgat
     8941 aatacccatc ttaatcaata agttgtttat gatgccgtta tgcccgagca aacccatcca
     9001 cgcatagaca cgcagcagga acgatgtcca aaaaggtaac atgatggcaa gcagcaaacc
     9061 attgcggacg gaaggattgg cacgggaaat cgcataggcg gtcgggtaac cgaccaacag
     9121 gcaaatcacc gtcgtcgtca gcgcagtctt aattgaagac caataagtca tcagatagat
     9181 attactgttt tcactgtcgc cgaaggggtt aagggtatgc caaaaatttt ggaaaatatc
     9241 ggcatagttt tgatagctga cggcgatgtt caggcgaccc aagtcctcat ctatcgtcgt
     9301 taaaggagta aacggtggaa tggcgatttc ttgttcggca gagctgattt tcagcacgat
     9361 ggcgaacgga atcagaaaca gcaccaaaag ccaaatatac ggtacggcaa tcaccgcacg
     9421 ctgccccgga cggcggaaca gtttgttttt cagtttatta aggttcatct cactcccctt
     9481 aaatcaacgg aacaacggag tcggttggtt ttccggccag ctgatataga cggtttcatc
     9541 ccaagtcggc ggcgtgatgt tacgcacata ccagtaaggt gcaggaactt ggcttttgac
     9601 gacgcgcccg ttggcgagtt tgatatggta tatggcgaag ctgcccaaat aggcaatttc
     9661 ttttaccgtg cctttcgccc agttgtagtc gcccaaatat tcgggttttt ctttatataa
     9721 atcaatatcc tctggtcgaa tactaaccca aagatcctgc tcgctcgggc cacccaaacc
     9781 atggtcgata cggacgtggt tttccaaacc ttcgcattcg ataacggcat agtcggcatg
     9841 attttcaatc accacaccgt caaagatgtt ggtttcgccg ataaactcgg cagtgaagcg
     9901 gctgttggga tagtcgtaca cgtcgctggg cgtgccgacc tgctgcaact gaccgtcgga
     9961 cataatggcg atgcgggtcg ccatcgtcat cgcctcttct tggtcgtgcg taaccataat
    10021 gcaggttacg ccgacttgtt ccagcgtatt gaccaactcg agctgggttt gctggcgcaa
    10081 tttcttatcc aacgccccca aaggttcgtc aagcaacagg attttcggac gttttgccaa
    10141 actgcgcgcc aaggcgatac gttgctgctg accaccggac aattggtgcg gtttgcgttt
    10201 ggcaaatttg gtcatctgaa ccagacgcag catttcttcg acgcgcgcat cgatttcgcc
    10261 tttaggcatt ttgtcctgtt tcagaccgaa ggcaatgttt tgctccaccg tcatatgcgg
    10321 aaaaagcgcg taactttgga acatcatatt gatggggcgg tcatagggtg caagtttggt
    10381 aatatcttga ccatcaagga tgatttttcc ctgattggga ctttccatac ccgccagcat
    10441 acgcagcagc gtagattttc cgctgccgga actgcccaaa agggcgaaaa tttcgttttg
    10501 ataaatgtcc aagtcgatgt tatcgacagc gtaattgtca ccaaactttt tcaccaaacc
    10561 ttggatttgc agataaggtt tggctgaaga cgctgtggtt gcggtcataa tggcaatact
    10621 ccaataaaaa gacgagtacc ggcaaaacgg attttcgaat gggtgataaa aagctgtttg
    10681 attgctggcg ggagtcgcaa cgtctgatgc cgtctgaaac tcttgtgaag cgcacgggca
    10741 gcatgaaata aaaaggggtt ctaaagaacc ccgcattata ttagtttata aggtttttag
    10801 ggtaataaat atgggtttga gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt
    10861 ttctgggcgg tgtcggaaag cgcaattatt tacgcagacc caagcgggta atcaacgcgc
    10921 gatacgtatc gggctgggta cggcgcaaat aggccaacag gcggcggcgt tggctgacca
    10981 ttttcaacag gccgcgacgg ctgtggtggt ctttggggtt ggctttgaag tggggggtca
    11041 gatcgttgat gcggaaagtc aacagggcaa cttgtacttc agaagagccg gtgtcgcctt
    11101 ctttacgttg gaaatcttta acgatttgtg ctttttgttc tacggtcagt gccataatgg
    11161 aaaaactccg aaaaataaaa agaaccccaa atgggattcg gacaagtttg ccaagcctcg
    11221 acaacggcaa actccctatt cctgaaacag gaacagcgca ttatcacaca atttttgctg
    11281 ttctgcacga tattttgcga tgtcggcgaa atgccgtctg aaaacggaga catcttccgg
    11341 tttcagacgg catttgacat cagaccacaa tttcttcctt ctgtttcgct tccttggcga
    11401 tattgtcgcg gctcaaaccg aacatcagca agagcgggct ggcaaccaat acggaagagt
    11461 agatgccgaa cacgataccg atggtcagtg ccatagaaaa gccgtgcaag gctgcaccgc
    11521 cgaacaccag catagaaaca accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
    11581 tcatcgttgc ggtaatcgcg ttgtcaatga cttccggcac ggtatgtccg cgcatcgccg
    11641 gcttgcggaa attttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
    11701 ccaatacggc aaggatacct gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
    11761 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
    11821 gccattcgaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
    11881 tcaatccatt atttaccaat tcctcaccga cttgcgggcc gataaattcg acttggcgca
    11941 aagtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
    12001 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
    12061 gaacctgtac atcacccatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
    12121 caccctgctg atattggact tccattaccg taccgccggt aaattcgaca gagaaattca
    12181 ggcctctggc aaccaaaaag aatacggcgg caataaaggt aaccaacgaa atgaaggttg
    12241 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
    12301 actccttacc tgccgcagtt tcggcttcag gtttccatac cgcaccgatg gaaatattct
    12361 gcaatttgcg gcggcggccg taccacagat tgaccaacgc acggaatacg acgacggacg
    12421 aatacatcga agtcagaata cccaagcagt gtacgacggc aaaacctctt accggtccgg
    12481 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttcgaa tcaacaatgg
    12541 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccggcacgca
    12601 attcctctcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
    12661 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catggcagac aaaataccga
    12721 ttaggaacag tatgttggca ctcaatgcaa tagtagaaaa gaaacccatc aggcggtaat
    12781 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
    12841 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggtg
    12901 cggcaagaga accggcacgc aacagcaaag acgtatcgtt ggcttcggct gtcgtcatgc
    12961 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggt gcggttacaa
    13021 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
    13081 tcagttcgcc gaaaatgctg ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc
    13141 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gccggtcagc tcgacctgtt
    13201 tgctgatcag cagagtttcg ggatgctccc cgccgcttga aagcagctca taaccgctcg
    13261 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atgcggactt
    13321 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcttgc acgccgggaa
    13381 gctgtacgac aatgcggtcc aaacctgact gttggataat cggctctgcc acacccaatt
    13441 cgtttacacg gttatgcagt gtagtgatgt tttgcttcac cgcatctgat cgtactttat
    13501 tcaccgcctc ttccgaaagc gccaatacaa tattgctgcc ttctgaattt aacgttgcct
    13561 caggaaacag cttgagtaac tgaggcagtg ccttttgcac atcgccggca tcctgcaaag
    13621 ggacggtcag gctgttttca gcctgacgca ccgtgccgct gcggattttt tcacggcgca
    13681 gttcgcggcg gatgtcgccc gaataacgct caaacgtttt ctgcatcgcg gctttcatat
    13741 cgacctgcat ggtgaaatgc acgccgccgc gcaggtccaa acccaaaaac atcggattgg
    13801 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
    13861 cttcgcccaa agtgttttcg ataacgtcgc gcgctttaag ctgcgtttct gtgtctttga
    13921 aacgcacttt cagtgaatta tccacaacaa acatcccgtc ggtctgaatg cccgcgtttt
    13981 tcagcgcagc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
    14041 tcgatacctg cacggcgggc gtttcgccga ataggttggg cagcgaatac actgcggcaa
    14101 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
    14161 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
    14221 aaaaacccct ccctgccagg caagaccgaa aggcagcatc ctgaatatgc cgccctgcgt
    14281 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
    14341 ccgatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttccact
    14401 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
    14461 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccggcttt caaggcggca
    14521 agcattgcct gatgcgcttt gaatttcttt tgttgggggc gcatgatcag gaagtagaac
    14581 accaccataa tcaacaccaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
    14641 ttctttctaa atatgattga aaatcgagcg gggtatataa tagcataaga ccgtaaacaa
    14701 tatatcgggt ttgccgccca tacccaaccg tatgccgtct gcaccccctt tcccgaaccc
    14761 atgccgctga cccgcctgtt cctgaaactc tacgccctgt tgcgcctttt tttgggcaaa
    14821 aacgcccaga ccgcctggat tttccacccc gcctgcaccg ggcacgaccc cggggcaaac
    14881 catcccgatt cgcccgaccg catcctctgc atcgagcagg cattgcgccg cgccggtatt
    14941 tggcagcacc tccaaaccgt agaggcggaa gaaatcagcg atacgcgcct tgcacttgtc
    15001 cgctcgagca aatatctgaa ccgtttggaa tcttgcctac ccgaagacgg caagatttgc
    15061 cgtttggacg acgacaccgt tatcagcaaa aactccctgt ccgccgcccg tttttccgcc
    15121 ggttcggcag ttcaggcagt cgatatggtc atgaaacgca aagcgtggca cgccttttgc
    15181 gccgcccgcc cgccggagca tcacgcgaaa agcggcaaag ccggcggatt ctgcctgctg
    15241 aacaacgttg ccgccggcgt catgcatgcc attgccgaat accgcctgaa acgcattgcc
    15301 gtcatcgatt tcgatgtcca ctacggcgac ggtacggcag aaatattcaa agacgatccg
    15361 cgtatcctgt ttttcaacct gtttgaaacc gaccttttcc ccttccccga aaacaacggt
    15421 atgcccgacg gcggcaatat ggtgcacctg cccttgccgt cgggaacggg cagccgcaca
    15481 ttccgcgaag ccgtccgcag acagtggctg ccccggcttg ccgcattcaa acccgaactg
    15541 gtgctgctgt cggcaggatt cgacgcacac cgtctggacg aatcgggcag gctcaacctg
    15601 cacgaggcgg attttgcctg gctgacacac aaaatcattc agacggcatc cagctgcccc
    15661 ggcaaaatcg tatccgtact cgaaggcggc tacacccttg aacctctggc acaatctgct
    15721 gccgagcata tccgcgtact ggcggggctg ggcaaatccg atgccgcaac cgcctatcag
    15781 aaaacactga acccaacaaa aaaacggttt gccaaaccga aaaccggaca ggtgcgccaa
    15841 cctacccaat ccgaccgtta cgatataaat gccgtctgaa gccgattcgg ttttcagacg
    15901 gcatcattat ttgacctgac gcaaacagaa agccttccgc gtgcttaaac acatctct
//
LOCUS       DAXDZG010000056        15299 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXDZG010000056 DAXDZG010000000
VERSION     DAXDZG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.056"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(219..344)
                     /locus_tag="ACFB78_001504"
     CDS             complement(219..344)
                     /locus_tag="ACFB78_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269474.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            384..620
                     /locus_tag="ACFB78_001505"
     CDS             384..620
                     /locus_tag="ACFB78_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC1269475.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            620..967
                     /locus_tag="ACFB78_001506"
     CDS             620..967
                     /locus_tag="ACFB78_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC1269476.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            975..1208
                     /locus_tag="ACFB78_001507"
     CDS             975..1208
                     /locus_tag="ACFB78_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269477.1"
                     /translation="MRSLSAGGSIRFHTRPYFKAFKISAGFQINAVKGAAHKTARKNR
                     RTDRYFSNKSNILRNSLKILVYSAYSYSFLNKD"
     gene            complement(1336..1668)
                     /locus_tag="ACFB78_001508"
     CDS             complement(1336..1668)
                     /locus_tag="ACFB78_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269478.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1669..2139)
                     /locus_tag="ACFB78_001509"
     CDS             complement(1669..2139)
                     /locus_tag="ACFB78_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269479.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2210..2515)
                     /locus_tag="ACFB78_001510"
     CDS             complement(2210..2515)
                     /locus_tag="ACFB78_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269480.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2627..6772)
                     /locus_tag="ACFB78_001511"
     CDS             complement(2627..6772)
                     /locus_tag="ACFB78_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC1269481.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLKQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRAMGGGDYTKQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            7012..7290
                     /locus_tag="ACFB78_001512"
     CDS             7012..7290
                     /locus_tag="ACFB78_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1269482.1"
                     /translation="MESQTFASVFDALCDTPAEAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPS"
     gene            complement(7318..7749)
                     /locus_tag="ACFB78_001513"
     CDS             complement(7318..7749)
                     /locus_tag="ACFB78_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFC1269483.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7751..8605)
                     /locus_tag="ACFB78_001514"
     CDS             complement(7751..8605)
                     /locus_tag="ACFB78_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFC1269484.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8602..9201)
                     /locus_tag="ACFB78_001515"
     CDS             complement(8602..9201)
                     /locus_tag="ACFB78_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFC1269485.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9201..9467)
                     /locus_tag="ACFB78_001516"
     CDS             complement(9201..9467)
                     /locus_tag="ACFB78_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269486.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9479..9808)
                     /locus_tag="ACFB78_001517"
     CDS             complement(9479..9808)
                     /locus_tag="ACFB78_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269487.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRREQEAAQA
                     GEARAAD"
     gene            complement(9869..10645)
                     /locus_tag="ACFB78_001518"
     CDS             complement(9869..10645)
                     /locus_tag="ACFB78_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269488.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAF
                     NSSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESV
                     TSGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10671..11099)
                     /locus_tag="ACFB78_001519"
     CDS             complement(10671..11099)
                     /locus_tag="ACFB78_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269489.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11096..11578)
                     /locus_tag="ACFB78_001520"
     CDS             complement(11096..11578)
                     /locus_tag="ACFB78_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFC1269490.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQREVKQ
                     NRLSGRVLGVRTGNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11578..12108)
                     /locus_tag="ACFB78_001521"
     CDS             complement(11578..12108)
                     /locus_tag="ACFB78_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFC1269491.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12111..12467)
                     /locus_tag="ACFB78_001522"
     CDS             complement(12111..12467)
                     /locus_tag="ACFB78_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269492.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAA
                     EPAEAEAEPAEAEKAE"
     gene            complement(12474..13973)
                     /locus_tag="ACFB78_001523"
     CDS             complement(12474..13973)
                     /locus_tag="ACFB78_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269493.1"
                     /translation="MNVNQLTQETIELMKSAQANGEPLNKGYTQPTSPTTGLQIYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRAIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKLGVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(14014..15171)
                     /locus_tag="ACFB78_001524"
     CDS             complement(14014..15171)
                     /locus_tag="ACFB78_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC1269494.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVNLSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
BASE COUNT         3205 a         4842 c         3917 g         3335 t
ORIGIN      
        1 tccgacaaac aggccgcacc acggggtatc tgtttctttc caccacgctt tctgctcctg
       61 attaaaattt cccatttctt gcagcatcgc taaaagcaca gggtttgact tgccattcac
      121 tttttcgtga gcgccaaggt atttacgggc ttcttcaatc catttcaatt ctttcatttc
      181 ttttccttcc gataaaagac gagatgtaaa ctttcggttt acaccccggc caagcctttg
      241 attgcaggtt tccgcaacac cgatacatcc ggtttcacac gttccgtttc catctccact
      301 cctgttacac gccgccgctt ggcaaaatcc gtttcaggcg gcataatgta tcgacaacgt
      361 atctacaaca cgaaagcaaa accatgttgc gactgcaaaa atggggcaac agcgcggcag
      421 tccgtctgcc cgcccgcctc ctgaccgccc tcaacgccaa aatcggcgac gcgctggaaa
      481 cagaaatccg caacggcgag ctgctcatcc gccccgtcaa acgctaccgc ctgtccgacc
      541 ttttggcgga aacggaagcc gccccgccgc gcgcggaagg ctgggaagaa atgcccgatg
      601 tcggacagga gggcgtttga tgtatattcc cgagcgaggc gacattttcc atcttgcctt
      661 cgaccccgcc gccggtacgg aaatgaaagg cggccattac gccatagccc tgtcgcccag
      721 agcttacaac cgcgcaaccg gcttggttta cgcctgcccg atttcacaag gcagggcggc
      781 ggcggcaaga agcggcggta tgatttccac actgctcggc gcgggaacgg caacacaagg
      841 caatgtccac tgccaccgga tgaaggcatt ggattggaaa atccgccgcg ccgccttccg
      901 cgaaaccgtc cccgattacg tgattgaaga cgtattggca cgcatcggcg cagtactgtt
      961 cgactgactt cagagtgcga agcctgtccg cagggggaag catccgtttc catacccgcc
     1021 cctatttcaa agcattcaaa atatccgccg gcttccaaat caacgccgtc aaaggcgcgg
     1081 cgcacaaaac cgcccgcaaa aacaggcgca ccgaccgata tttctccaac aagagcaaca
     1141 tacttcgcaa ctccctcaaa attttggtat actccgccta tagctattcc ttccttaaca
     1201 aggattaggt taagaaacgc cgcaaggatt ccgcccctcg cggcgtttcg ccgttcacac
     1261 cccgcgcgta atgtcgtgcg tcagccggac gcgcccggaa cacagcgtct tcacgcgccc
     1321 cgataccgct cgaaatcaat cttttccatc cgaagaccct ccgtctttca cacccgtaag
     1381 cttttccgcc aagccgccca agtaaccgcg caacaccttg ggcgcgagcg cgcgcaccgt
     1441 atcgagcgcg tgccccgtca tcatccccgc gaacaccccc gccgccgcgc acgtccacgc
     1501 ctgatccagc ggcacgaacc gctccgccgc ggccgccgcc gcgattgcag aaataaaagc
     1561 ctccagcaca gcctgcaccg ggcggccgcg ttcctgaagg cttgcccaca ccgcgcccag
     1621 cacgccgccg gccgcggcaa acagcagtcc gaactgcaag aaccccattc acgacccctc
     1681 atccgccaaa ccccgcgact tttgcgccga aaacaaaaac ctcaacgcat tattccccgc
     1741 cagcgcgcag aaaaacgcca gcagcggcgg aacgaccata cccgtgttca aaggcgggta
     1801 cgccccccaa aacgccaccg aaaccaaaaa ccacacaaag cccgaaaaca gcaaaaggta
     1861 ggccgaagcc acacaagccc tcacgccccg cgcaaacagc aatgccgtct gaagcagccc
     1921 cgccgccgcc agcaccccca ccgtccacgc ctccggaata tcctggaatt tgtaataaat
     1981 ccgccacgcg taaatctcgt cgggcgcaag cgcgaacacc gccgcgtaac ccgacaagcc
     2041 caaaccgctg accgcctcca gcacccgcgt cgccgtgccg aacaaccacg cctgaaaccc
     2101 ggcgggcaaa aaccgccgat tcaacaaaac cctcaacata cccgtcacac accccccggc
     2161 aaaacaccgc gaagccgaag ccccgcagtg tccgacgccg tctgaaaact tattccgcgt
     2221 aaaccggttg cgcgtctttg gggatgcccg ccgcaccttc caacgccttg cggtacaccc
     2281 aaccggggtc gggcgcggtt atgtccgttt cctgaaaatc caatgtgcgc gccaacaggt
     2341 agcgtttgcc cgatttaaac gcattttccg acacatagcc gttcaacgtc gccgtaacat
     2401 aaccgttgcg gtaatccgcg ccgatatgtt cgacgacgtg gtaggccgca ctcgcgccgg
     2461 tcataccgtc ctcgactgca aaattcaagg caacaatctt tttatcttca atcatttcaa
     2521 atctccaaaa gcccgctagg ggcgggaacg aaaatgccgt ccgaggtctt cagagggcat
     2581 tgcccggaca aaacaggaaa aaccgcagag ccggcatacg ggggcgtcag ccaacctgcc
     2641 aaacctcgtt ttcgcgcgat tccggaatca gcaccctgat attgcgcagc tcgatatact
     2701 gccaccagtg ttccacaaaa aacagcaggt tggatttcgg aacggcagta ttaaactcct
     2761 cctgaaacag agaatactgt tggggcgcat gaccccggta tttcttaacc actacaaact
     2821 tatccgacct gtcgaacgca agcatcgtac tccagtcggc attggtaaaa ggccggtact
     2881 cgaacgatat gccgtagata tcgtccggca actgaagcag cccgccgtaa gcattccaat
     2941 gtttatacat ctgattttgg taatcgtgta cacgccggtc gccgcccaaa taaacctgct
     3001 tcttactgtc gtaccggaaa ctttccgcaa aggtgcgcca aaccatcctc ccgagcagct
     3061 gcttgtattc gtagtcggat tgcgccaaat acgaaaccga cacgaacggc gaaaacaccg
     3121 attcgtggga agcgagcgac aacttcaggc tgacgggttt gcccgcgggg atgacttgga
     3181 tctggtactc gaggcgggtt ctgtaccttg agccggattg tttggtataa tccccgcccc
     3241 ccattgcccg ggggggcagg tcttcgtacc tcccgaaagt tcggccgcgc caaatataat
     3301 tggtatagtt ctcggtttcc ctgacttccc tcggcgcaag cacacccccg ttcaacagca
     3361 gctttgcctg caccctgttg ttgccgaacg tttccgaaac cacataaaaa ttcggaatca
     3421 aaaccctcgg cagcgggtct ttatccaaat ccaacaccca aacgttaccc tccgtccaac
     3481 gcagcctgtg cgccctcaac acgtcgccct cgacggacga agccctgacc gtgccgttga
     3541 aataacccga atcggcctca atccgtccgc gtattaccgc attgtcggcc gtcaggatgc
     3601 cgtctgaaga caccgtaaaa ttacccccgc cgatattcag gctgccgccc gtgatgcttc
     3661 cgagattgga agataccgcg ctcaaatcgg caacctgaat cttatcggca gttaccgcgt
     3721 ttgccccgat tttatcggcg gtaacggcgg cagccccgat ttcccgcgcc gtaacgcttc
     3781 cggctttcag gcggtttgcg tccagcgtgt ccgccgcgat tttgccgccg tggatatccc
     3841 cggcgttcaa cttatcgaca accgccttgc cgttgaccac caattcgccg ttgatgccca
     3901 cgcggttttt ctgcgtgtcc accacgaacg ggaacacatc cgccttcccg gccgcaccga
     3961 tgccgaagcg gtcggcattc acgataaact tgctttcggg cgtgccgttt ttcggcgtag
     4021 tcgccaagcc gaagcccgcc actttgccgt ttgcatcaac cttgaccgtg tattgcgcct
     4081 ccaagccgtt gatgcttcgg gcgtgtgtct gaaccgccgc cgcattgccg tccgccgtcg
     4141 atcggactgc ctcgacgcgc tcgttgacgc tgcccgcgcc gttgccgtca atcaggttga
     4201 ttttgtcgcg caaagccttg ttcagactgc tttccgacag gtcggttgcc gatacgtcgt
     4261 aaacggtaaa cgatatgctg ccgctgattt tcaggttatc tttgccgaaa ctgtcgtaac
     4321 ccgccgccct caagtaatag gtctttcctt tctccagcgg tttgccgccg catttggcga
     4381 tggttataaa cgtttccgcg ccgtcatagg ctttgtttgc gtccgcggcc gggcaggctg
     4441 cgttttcgca aacccagacg ataattcctg caaagtcttc ttcgtcaggt ttttggcagg
     4501 taaaaaacgc ctgtttcaaa ccgctgtcga tggaaatacc ttgtaatgcc tgcagttgcg
     4561 gattttgcgc cgcgatttgc gcccaattgc ccgttttacc ggtaacggca cgcccgcgaa
     4621 ccttgaagac aataccgcgt acctggccgc cgtccgcctt catatccgcc tgagtgtagg
     4681 tgtagctgtt gtcaacgatg ccgtcaaccg cacgcagacg gcgtcggctg ccgcctgcgt
     4741 agatttccac gtcgtaggta tccgcgccgt ccaatttatc ccaagcgatg acggcttctt
     4801 tgccgtatgc ccacgatgat gtcaggcgca ggttttggat ttgaccgagc ggcgcgcctt
     4861 cgatggtgta ggaatacgcc ggcacttccg ccaaatcctg aatgccgccg ccgaaaacgt
     4921 tgtacgaaac cagtttgacc caaaccgtgc ggccaatcca gttgcgcgga acggcgtatt
     4981 tgaacaatgc ttcgtcgatg cgcgcaaacc ggctgcccgc cgcgtgtgcg ttgacggcgg
     5041 agccgtacgc gccgcgcgtc aggttgccca atgtgtagcg tcccacgccc ttcagttcgg
     5101 cgtcggcgta tgccagaaat tcgccgtcga cgtagcacaa tgtcagcaaa tcgcggctgt
     5161 cctgctccgt gccgcccgtc agctgccccg ccgaaatttc cacgcccaac gtatttgtgc
     5221 ggtcgaaaac cgcgccgtcg ggcaaatcgg cggtcagcgc gccgaaacgc gccttgcggt
     5281 tgaccgcgcc gacgcgggtg tagctgtcgc cgtccgccga tacccacact tcggcgccgc
     5341 cccacatacc gcccccggcg gttgccagcc agatttgcgg ttcgccgccc gtcagttgca
     5401 acggcgcttc gaaaattacc ggcacatggg cgttgcccgg cgaaacgttg tagtcggccg
     5461 aataacccga agacggctgc gtcggatatt ccgacacggt ataaacaccg gccgggcagt
     5521 cttcggcctt gacggataaa accccttctc cgtcttcttc gatttccgta atgcggacgg
     5581 gcgttttatt caggccgagc cccgcgtcgg tcagggttac gatgtccatc ggctcaagca
     5641 ggcagtattt ccaacccagc ttaaactcat attcgttgcg cacgtacagg gcgcgttgca
     5701 gcagttgttg cgccactttt tgcgccacct tcgcatcgca aatgccgtgc atcctgaccg
     5761 cgtctttcgg gcgcaatccg tactgctcga tattcgcctg gtctttcgct tcggcgatgg
     5821 cgacgttgta gtcgttgtcc ctgtcaaggt actcgacctg aatctgatta aacgcatcgg
     5881 cattggtttt gcgcccggcc tttacagggt cttgcgcgcc cgaaacgatg aaatcgtcat
     5941 cggtcaggtc gtaggcggcc ttattgtcgg caacgtacac cgcgccgttc cccgaatggc
     6001 tgccgtcccc gtaggggatg attttcaggc ggccttgcga aaataccgcc gcgctgttgg
     6061 tctgctccag cagttcggaa atattccgtt gcgcctcccc ctgttccgtg taggcagggc
     6121 ttaggaaaat cccgacggcg cggcaataat tgctgtaccg gtcggtatcg ccgatgtttt
     6181 gggacgggaa accgcagccg tagcgttggt tcgtcagcaa atccaatacg atttcgcgcg
     6241 ggtttgcatc ggggatgttg ccggaatagc ccgatttccc gatgacctcg aaattgtgct
     6301 gatatatctg cgccgatttc gtcagttcgt agttcgggct gcacaaataa gccgtgcccg
     6361 aataattcaa ggcttggcct tggtgcttcg cctgttgcag gtgcgtccac aacggctgct
     6421 cgtcgccgcc gcgcataagc gtcaggcgca actgtgccgg cgaatcgaat ttctccttgt
     6481 cgcgccaaac ccgccccacg ccctgaatct cgccctcgca caaagcaagc atgacggcgg
     6541 cttcgtaggt ataggaaata ccgacctgtt tcacaccgcc gccgcccttg ccgccccggc
     6601 gcgtcgtagt tttatgctcg atggtgacga aatcgccgta ccaaatcaaa ttgcccgcca
     6661 cgcgcgtcct gccgtagatg acgggcaggg tcagcccctg tgatgaccgc tgaacctgta
     6721 acgataaaat ccgctcttcg gcagatgtaa cggttgacga tttaccaccc ataaaaccac
     6781 ctcgaaactt gcaaaaatta tcaaaatcga tataatttac ttatatcaaa acagatataa
     6841 aatatgaaac cgttaaattt ttcaggcggt tcattggatt gcctgcggca attccccgaa
     6901 aacgccaaac aggcggcagg ttatcaactt caagaaaagc cggaaaaccg cgggcgccga
     6961 ttcggaatcg gcggaaaaac gttacaacaa attgattcgg gaaaagaaat aatggaaagc
     7021 caaactttcg cctccgtatt tgacgcactg tgcgacacgc ctgccgaagc cgccaatatg
     7081 cggttgcgcg ccggcctgat gatgcacatc gccgataccg tccgcgaaaa cggttggacg
     7141 caaaaacagg ccgcagaaca ttgcggcctg acccggccgc gcatcaacga cctgctgaac
     7201 gggaaaatcg acaaattttc attggatgcg ctcgtaaaca tcaatgccgg actcgggcag
     7261 tgcatttcct tatccttcgc cccctcgtaa agcccgtcct ttcagacggc cgcaaaatca
     7321 aaatgaaaat gtaaaaaacc gaaccccgcg cccgataagt tcggcttggc cgatgtcgtc
     7381 cgacaccacg ccgcgcccga tgtagctgtg aataaccttg ccgccgcccg ccaatatgcc
     7441 gccgtgcgaa aacgaccgcc cgaaacgcca tactgcaatg tcgcccgcct gcggcgattc
     7501 cgtttcacgg caaaattgcg tgacgaaccc caaataacgc tcgcaatcgc ggtgcagatg
     7561 ccaatcttga gggtaagggc gcgggtcgaa cccttcggga agcagtccga ccgccccgta
     7621 gacggcgacc aggagcatcg cgcaatcaac acccgcgccc ttgaccattg catgatgatg
     7681 gtacggcgtg ccaagccacg accgcgcctc ttcgacgatc cgcgccctca aatccgtttc
     7741 ggacggcata tcacaccacc gtatctgccg aagggatgaa cgggaagccg cgaaaatgca
     7801 cgatattgtc aaacttatcc ttgcaggtat cgcgccgctt gtcgcagccc ggataaacct
     7861 tgaacgcatc gcccgcctgc ggcgggtagg gcaggcgaag ggcaagttcg aacgtgttgc
     7921 cgccgtgtgc cttgaccgtc ctgctcaaac ccgcgttgcg cccgcccgca aacttaatca
     7981 cgccctgcga aaaccagccg tcaggctgcg tcagattgtg cttcagcacg gttcccgtcc
     8041 ggctgttttc ggttacgcgc ccgtttaccg tgaatttctc acggttgacc ttgcagccgt
     8101 cgtcatagag cgtcctcatg cagcccgcct gataaatgtt gcgcgggctg gaaacgttca
     8161 aaagctcgat gtcggatttc acgtcaacct tcacggacga cctgccgccc gatacgtccg
     8221 acacgcgccc tgaaaaaata ttcaccgcac cgacggggcg cagtccggca tcaaaaaaca
     8281 cacggtctat cctgacccgc gcgccgtcca atacgccgcc cagggcggcc tccgcccatt
     8341 gcaggccctc aagcctgtaa tcgggcgcgg ctgaaatctg cagggtgttg gaatccactt
     8401 ccaatccgac ggcggtacgg gttgcgccgc gcttgataac cagctcgtgc gccccgtagg
     8461 cctgaccgtc ccaaacgacg ggcatatccg cgccggtatg ccgcagcacc cggccgcccg
     8521 aaagcgtaat ggtgtacaaa tccgccatct gaaactcgtc gccgccgtgc agcaaatcaa
     8581 tcagttcttt tgtcgccgcc ttcatagttt cacactcgta aactcaatct ttttggccgc
     8641 ccacaagctg cccaaaacgt tttcaaaatc caccgtgtca gacgtaaacc tcacgcggaa
     8701 ataaaagccg cccgtccatg tgatcgggcg gcccggcggt tgcggcgtgt tgaaaaccaa
     8761 aacgcccttg tcggtaacgg tgtaatcgcg cccgtacgcc aacgccgtgc cgccgacctt
     8821 gacggcgggc cgttccttga ccgccgacac aggctcgata aatccgccca tcgaacggac
     8881 aagctgataa cgcgcaacgc cctgcaccgt gtttccgaca ggctggtcgg ttacggcgtt
     8941 gtcggccggg tcttcgtaaa ggaaactttc aaagctgcct ttgcgggcgt tgaagaatcc
     9001 cgccagtttt tccaactcgt ttacggacgc ctttgtccgc aatacctcga acgacagcga
     9061 aaaccgccat tgcgggtagg tgtagtaggc ggttcggaac tcacggccgc ttgccgattt
     9121 ctgcgtcccc gtactccata ccgccgtttt cttccgcccc cacttcaagc cggggaactc
     9181 ggggaaaacc gcattgccca tcagatgatt cctttcgctt tgagcagtgc gtcaaattcg
     9241 tcttcggaca gttcgccgcc gccaagcata ccgacggctt cggcttcgtc cgcttcgctt
     9301 tgcacactgc ccgacgacgg cttgacgccc atatacgacg ctgccaagat atgcacgggc
     9361 ggatgttcgc gccaatactc gttcaggtgt ttgatacgcg gcaaatccaa gttgtcggcg
     9421 acgtagtccc acgtccagcc ggtagaggcg caaacgtggg caatcaccgc gccgaaactt
     9481 aatccgccgc ccgggcctcc cccgcttgtg cggcttcctg ctccctgcgt ttcaagcccg
     9541 aaacgtccat cacggcggca aacacttcgt tcatgttgcc gatatcgatc aaatcggccg
     9601 cttcttcgcg cgttaaatcc gggtaattgc gcttcagggc ggcgtgggcg caatcgataa
     9661 cggtagagat ttgtccggca tccgccgcat tgccgtcaaa tgagccgatg cgctcctgca
     9721 actgctccaa cgcgcccagc gcaataggcg gaatcacata atccgcgccg ttcagttcta
     9781 cggttacgcc tttaatccgt acggtcattt ttatttcctt gactttggtt gaataaaaaa
     9841 agccgccttt tcagacggca tataaacatt actcctgaat ccacagcgta ccgattttaa
     9901 agcccgcctc gtcggttgag gcggtaaagt cgatttcggg gacggagaag tcgtcgtttt
     9961 tagtcgagaa caagcccaat ttgccgctgg ttacgctttc cagttccaac agggcttttt
    10021 tgcctttgaa ctgcgtcagg tacctcatct taaacgtcgg cgtgttgccc gtcgccatat
    10081 tggtcagctc gattttcttc gccgggggca tcgtttgcgt ataggtaaag ctcggataaa
    10141 cggttttgcc tttatccgag ctgttaaagg cgtacacgcc cgattccgaa accgtatatt
    10201 ggccttgttt cggggaggat gctactttga tataagccgt gccgtcctgg cccatcacgc
    10261 ccgcgtcctc agcgaacttg ccgccgccgc cgggcgcggc tgcctgaagg taagaataat
    10321 tgtcgccatc gaggactttg cccgtagttt ccgcccaaag cgccttcatc gtgccggttg
    10381 catattccgt accgaaaaac agggtattga gggtcaggcc gttaatcaac gcgcctttca
    10441 ttttgcccga caccttgacc ttgccttgcg ccacagccaa aggataacgg ttttgaccgt
    10501 agaactcttt caattccgcc gacaaatcga cggacatttc ctgcaagccc ataatccgca
    10561 cgggcgttgc gttctgtaca cggttgccgt aagcatccgt aatcatttgg gcgaacactt
    10621 cgccgctgcc aaacgtcaac tgcatgacat ccctttcaaa aaaagccgca ttacgcggcg
    10681 caaatcacaa tcggaataat acaaaccgcc tgttcgccaa gcgtcccctc gtcggtttcc
    10741 accgtaccct cgacgcggca atactcgata tccgcgccgt ccgccgttaa atccgtcttg
    10801 cccgtaacgg ggtggacggc gttcacggca ttgcacaccg cgtcaatcag cggattcatg
    10861 acgggcgcgg gcggttggcc cgccgtctgg acgtacaggt aaacatcggc gcgcaaaatc
    10921 cacttggtct cccgccccgt caatgtcaac gcctgcatat cgccctgcgc cataaataac
    10981 gcgggctgat cgtagcgttt cacatcgttc cagtgcacca gcttccggct cttggttacg
    11041 aagccgtcca atgcgtccag cttcgcccac agcgcggaat aaaccgcctc acgattcatt
    11101 tcaacacctt tccaatcgat ttttgcaggt cgtccgcgaa cttcggcgtc aaatcgcgca
    11161 atgccgaacg gaggaacgac cgctccggca acttcacatc acgggtatgg gcgcggacgc
    11221 ggacatatcg cggcggtttc agcggcttgc cgaacgcctg cctgacctgg cgcagcgaag
    11281 ccttaacgtt gacactgccc gcgaaaccat actcatgcgc gatgccgtaa cggacgttcg
    11341 tatcgacttc gcccgaaacg acattgccgg aaacgttcac gcgctggtgt acggagcgtc
    11401 gcaggttccc cgtccttacc cccaacaccc ggcccgataa ccggttctgc ttgacttcgc
    11461 gctgcaggcg caatgccgac ctgcccaccg atttgacgac cgcatcttga acatcggcgg
    11521 catatgcttt aaaaaccgcc gccagaatac cgccgccgat aaattccatc ttcagcatca
    11581 cacgcccttt cgtttgtact cattgagtat cgcaaacgcc gacggcggca taccgccgga
    11641 attaccgaac gtagaaaaag cgacggtttc gcctgcaagc gtcttgctcc gtacgccctt
    11701 gccctcgatt tcgttcacgc gctgcaccgc gataatcatc accgcttcgc gtatgtcggc
    11761 gggcatgcgc tcataaccgg cgcggtacga tacctcgaca tttttcagcc cccgcgcgaa
    11821 accttcgggg cgcatcagca gccagttgtc aaactcccag ccgccggcat ccgcgccgtt
    11881 gattttgacg gacgacaccg acaggacggg ataattgtcc aacacgatgc ggtctttgcc
    11941 gttgccgtcg taccgcccga cataatcggc ggccaggagg ctgcgcccga tataggcttc
    12001 cgccgccgcc gacacgccgt caatgacgct gaggaagaaa tcgtcccgcc ggtcatgttc
    12061 aacgccgata cgctgcttga actcctcaag cgataccggg gcggccatcg ttattcagcc
    12121 ttttcggctt cggcaggttc ggcttctgct tcggcaggtt cggcagcttc ggcttcggca
    12181 ggttcggcag cttcggcagg ttcggattgt cctgccggct gcccggtttt aggtttacgc
    12241 ccgcgcttgg ctttttcagg cccttcggca ggctcggagg caacgttgcc aaaaccgaac
    12301 tgatacagga attccgccgc ctccgacggc acttccacga taccgttttc atctgccgcg
    12361 tagctttggc tgccgaagga aacgtcggta aacccttcgg gcgcttttaa tttgactgtc
    12421 atctcaaaat ctccaaaaga aaaagccgtc tgattttcag acggcatatc ggcttaaccc
    12481 acgttggtaa tcataccgaa ggcaggcatg aacatacctt gcagcacctc gtccgcatag
    12541 acgccgtatt cgtacatacg ggtgcgcagc ggccattcga tttggtaata ctcttggcgc
    12601 gtacgcactt gcagcagatt gccgacgccc tgaacgtagg cgggcaggcg gcttgagtag
    12661 aacaggtaag taccggcagg caggttcggg tgtaccacga tgttcaggtc gtcgcccgtg
    12721 atcttgttca tatacgaacc gacaacgaca ccggcgcgga tgttcgccgt attgttcacg
    12781 tcaacgttca acttaatcaa cggcgcgccg ctgtttccga taatcagctt ggtcagcgcc
    12841 gccaaatcgc gggcgttgac gtagacggta tcgggggaca ggcggtatcg ggtaaagaaa
    12901 tgcgcgaacg cctcttcaaa ttcatacacg ccgcccgcgt tgtcggaagt caggccgccg
    12961 cctttgttgt ccgaccagaa cgcgcccgaa tcaggcaggg cgatttgggt caacaggccg
    13021 tcaaattcca aaatagaagt ggaattgtct tcggacggca gggaagcggc ggtctgggta
    13081 ccttcggcat cagccgaaat ttccactttg gcggcagtgg taaccgcgcc cagtttttca
    13141 gaaccggccg caccccagta ccaagcatag gcaaccgcgc cgcgaacagc cggaatcacg
    13201 gcggttactt ttttgcctgc ctcaataccg gaaacggaag ccgccgcaga ttttcgggcg
    13261 gaaccgccgc cgaacgtatc ggttgtacca tccgcgtttc ggcgtgtgat tttggcaggg
    13321 acttgggcag ttttaatgtt cgggctttgt ccggttgcac cgttgttcgc gcctgccaca
    13381 tcccagtatg cctgcaagcc caaagccaca cagattacag acaaggtgct gacgccgagt
    13441 ttgcccgccg cgtcggcgga aacgacggcg gtcggtgtag gtgtaacgcc tgccttcagg
    13501 ctggtgttgc cgcccagcaa aatcatttct tcggcaacca tagtagcctg aagggtttgg
    13561 gcgaccgcca acgctttcac gtcctcgaaa ccgcgcgcgg cgtaatccgc ttcaaaggtt
    13621 acttggtttt ccaagccgat ggcgcggaat tgcgcgttgc gttccaccgt ttcgtgattg
    13681 atgacgccgc cgcgcctgcc ttcgccgata ccggcgcgtt ggttgccgac gttgatattc
    13741 gtgacggctt tccagttcga gccgatggcg cggccgccgc ccacgcgcgg gatacggttg
    13801 cgcaacgggg tcaataccgg atagagcttt tgagacggcg cggacaggtc gtagatttgc
    13861 aggccagtag taggactggt aggttgggtg taacctttgt tcaacggctc gccgtttgct
    13921 tgtgccgact tcatcagttc aatcgtttct tgtgtgagtt ggttcacgtt catttatcgc
    13981 tcctgataat aaaaaaaccg cctgtaagcg gtgttacaga cggcctgttt gttttgcctt
    14041 aatgagtgtc gccacgtcat ccagcgtgcc gtcattcttt acaatcggct gaaaaccttt
    14101 taacgggtct tcgccgttat cttctgcctt actgatagct ttcgtactgc ctttcggcgg
    14161 tactgcctgt ttcttcaggc tttcgatttc cgcctgcgct ttggcaaggg cgtcattcga
    14221 ttttttcagc gcgtcttgtg cctttgccag ttcgtcagcc gattccgctt tggcaagatc
    14281 gcctgatttg ccggctttgg ccgccaaacc gtcggccggc ttatcggctt cgcttgccgc
    14341 caacgctttc agcgattcgg caaggccggc cgccgattct ttgatttgcg cggtaacggc
    14401 ttcatcgatg ttgtcgtagg atgcgtcctc aaccagccat ttcagcgaca tcaatacatc
    14461 agccggtgat ttgacttggt acattgattt ggcggccgtc ccgcctttcg gcttgtcggc
    14521 tttagccaat accgctttca aggcggcgat ttcagattcg gacaaattca cgcttgccga
    14581 ttttccggct tcgtcctttt tgccggcgtc cttatcgcct ttatcttcgg ttttaggccc
    14641 tttatcgccg tctttaggat tttcatcttc ctcttcggtt ggtttgtcag acggcttgcc
    14701 gtctttatcc gccgcccctg cctcatcttt cggtttgtcc gccttaaagc aggtaaacac
    14761 cgcgtcggga ttggcagggc ggtcgacaag gctgatttcc gtcagcttca agcccgtgat
    14821 ttgcgacttg ttcaaatcat cgcgggcggt aaccctgccg ccgatggaaa agcctttgta
    14881 aacgcctgtc ttgactttcg tcacggcaac agggtcaacg atatgcgccc cgaaaaatgt
    14941 gcgcccgtcg tcttccacgt tgatttcaat cgccgttccc gccgcgtttg agccgtgcat
    15001 ctcgcgcacc gcgccgaact tcatataatc gggaatcgcc gccttcattg cttccgccgc
    15061 gacgacttcg ccgtccgaat cgaccgcttc gctcgaggca tacccccaaa ctttgaccgt
    15121 gccgtcgtcc tgcgcctccg tcttggcgat ttgcgcgtat aacttcgtca ttggtttgct
    15181 ccaaaaaaaa gccgccccgc gaaggggcgg caaaacacac ccgcctgacc cgaaggaatc
    15241 aagattcagg catatcccct gccaaaacag gcacaaccgc gcaccggcag ttcggatga
//
LOCUS       DAXDZG010000057        14596 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXDZG010000057 DAXDZG010000000
VERSION     DAXDZG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.057"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            760..1869
                     /gene="fic"
                     /locus_tag="ACFB78_001525"
     CDS             760..1869
                     /gene="fic"
                     /locus_tag="ACFB78_001525"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC1269495.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2584..3396)
                     /gene="murI"
                     /locus_tag="ACFB78_001526"
     CDS             complement(2584..3396)
                     /gene="murI"
                     /locus_tag="ACFB78_001526"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC1269496.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3585..4046
                     /gene="tsaE"
                     /locus_tag="ACFB78_001527"
     CDS             3585..4046
                     /gene="tsaE"
                     /locus_tag="ACFB78_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC1269497.1"
                     /translation="MSDFSPVPRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4043..5293
                     /locus_tag="ACFB78_001528"
     CDS             4043..5293
                     /locus_tag="ACFB78_001528"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC1269498.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5375..6193
                     /locus_tag="ACFB78_001529"
     CDS             5375..6193
                     /locus_tag="ACFB78_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC1269499.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPYV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6239..7380)
                     /locus_tag="ACFB78_001530"
                     /pseudo
     CDS             complement(6239..7380)
                     /locus_tag="ACFB78_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
     gene            complement(7429..7851)
                     /locus_tag="ACFB78_001531"
     CDS             complement(7429..7851)
                     /locus_tag="ACFB78_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269500.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GNWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7844..8653)
                     /locus_tag="ACFB78_001532"
     CDS             complement(7844..8653)
                     /locus_tag="ACFB78_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC1269501.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8735..9112)
                     /gene="acpS"
                     /locus_tag="ACFB78_001533"
     CDS             complement(8735..9112)
                     /gene="acpS"
                     /locus_tag="ACFB78_001533"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC1269502.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFCNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9162..9890)
                     /gene="pdxJ"
                     /locus_tag="ACFB78_001534"
     CDS             complement(9162..9890)
                     /gene="pdxJ"
                     /locus_tag="ACFB78_001534"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC1269503.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAH
                     SHAEQIRQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(9943..10686)
                     /gene="recO"
                     /locus_tag="ACFB78_001535"
     CDS             complement(9943..10686)
                     /gene="recO"
                     /locus_tag="ACFB78_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC1269504.1"
                     /translation="MSEHRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10720..11847)
                     /gene="pheA"
                     /locus_tag="ACFB78_001536"
     CDS             complement(10720..11847)
                     /gene="pheA"
                     /locus_tag="ACFB78_001536"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC1269505.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIRHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(11886..13115)
                     /locus_tag="ACFB78_001537"
                     /pseudo
     CDS             complement(11886..13115)
                     /locus_tag="ACFB78_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(13231..14106)
                     /locus_tag="ACFB78_001538"
     CDS             complement(13231..14106)
                     /locus_tag="ACFB78_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC1269506.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
     gene            14289..14596
                     /locus_tag="ACFB78_001539"
                     /pseudo
     CDS             14289..14596
                     /locus_tag="ACFB78_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         3389 a         4187 c         3915 g         3105 t
ORIGIN      
        1 tgccctctct ctaactctct cccacgggga gagaggacta tgatgcccac cggcggcttg
       61 ctattaggaa tatctgaatc gtcattccca cgaaagtggg aatctagaac gtcaaatctc
      121 aagaaaccgt tttatccgat aagtttctgt tccgacaggt tcggattccc gcctgcgcgg
      181 gaatgacgaa tccatccata cggaaacctg catcccgtca ttcccacgaa agtgggaatc
      241 tagttttttg agtttcagtc atttccgata aattgcctta gcattgaagg tccggattcc
      301 cgcctgcgcg ggaatgacgg gatttgaggt ttctgttttt aattttctgt tttcacggga
      361 atgacggttt aaaagttacc cgaaacctca aaaaaaaaag ctgtgtttta atatagtgtt
      421 gatagacgta cttggcctcc atgtattcaa tcgtggaaat ctatatcttc gtcctcgccg
      481 aaatagtcta tgcccgatat acaattttga tacacaaact tggaaatatg ggtatcgtcg
      541 ccggagcgat agaatgcgga cagttatata tatacggttt ttttagggga gcgggcagat
      601 gaagggcgga ttttgaatta accccatgca aattgacttt tgcgggcggt tttaccgccc
      661 atatatttac aaaagccaaa tttctaaaca tatatccttg atatatacac ggcgtaaaca
      721 tatactggaa acatctttaa attttccgaa attttaaaca tgagcaactg gaaacccgat
      781 attccctata acgatttacc acccctgccg ccaaaacagg atattgaaag caaaaccatc
      841 ctgaaacgtt gtatagccgc ccgtgcatcc cttgcccgtt taaagcaggc ggcagaattg
      901 ataccgaatc aagccatgct gattaacacc cttcctgtta tggaagcccg tgcaagttcg
      961 gaaattgaaa acatcgtaac caccacggac aagctgtttc aatccctgca aatggatacg
     1021 gaacggcaag accctgccac gaaagaagcc ctgcaatacc gcaccgccct gtttgcaggc
     1081 tatgaatcac tggcgagccg ccctttatgc acacaaaccg ccatcatggt ctgcaacgcc
     1141 atcaagcacc cctacgaaac ggccatccgc aaaacaggcg gcacagccct aaaaggaggt
     1201 aacagcggaa atgttgtcta taccccgccc gaaggagaag aaaccatacg cggcaagctg
     1261 gcaaattggg agcggtttat tcacgaaagc ggcgatttag acccgcttgt catcatggcg
     1321 gcggcacatt accaatttga agccatccac ccgtttacgg acggcaacgg gcggacggga
     1381 cgcatattga acagcctgct attgattgaa aaagggcttt tggatttgcc tattttgtat
     1441 ttgagccgct acatcatcga aaacagggcg gactattacc gcctgctttt aggcgtaacc
     1501 gaacggcagg attgggaaag ctggataatc tacatcttag acggcgtagc cgacaccgcc
     1561 gattggacgg tatcgaaaat agatgcgata cgctgcctgt tcgagcagac acgtcaacac
     1621 atacggacac acgcacaagg aatctacacg cacgaactgg taaatcttct gtttgagcag
     1681 ccatatacac gcattgccaa cctagaagcg gcagggatag ccaaacggca gacggcttct
     1741 aagtacctga aagagctttc aggcataggt gtgctgcaag aaatcgccat cggcagggac
     1801 aaactattca ttcatccgcg cctaatggaa ctattgcggg gagagggcaa cagctttact
     1861 tcattctaac cccctcttca tccctacatg actaacacga aacagggatt ttgacacccg
     1921 aaccggtacc ccttgtattt tccccgcgaa aagccggcat ccgcccgcgc atcatgggag
     1981 caacaaaacc cctgcctaaa attttgactt gtgcaaatta ggggtatatt gaaaaatggc
     2041 taaaataaaa tgtttaataa acaaaatgtt gaaacttaat ttcgatagag cctctgcata
     2101 tcgtattgag gcgttaatgg aatttgagaa agctattttt aaataagaaa aggtaactat
     2161 taaatagcta cctttctaaa attaaatatc aacacatcgt taaaacacag ccaaaaaaaa
     2221 ccgaaaccga acggtccgga ttcccgcctg cgcgggaatg acggatttca agattacggt
     2281 attgtcgggg atgatgggaa acggtgggaa ttgtgtaaaa aatgccgtct gaagcctttc
     2341 aggcggcatt tgcggcattc ggacatttaa aaatcccgtc attcccgcgc aggagggaat
     2401 ccgaatacat ccgcacagaa acctgcatcc cgtcattccc gcgaaagcgg gaatctagaa
     2461 cgtaaaatct aaagaaaccg ttttatccga caagtttccg caccgacagg tctagattcc
     2521 cactttcgcg ggaatgacgg gattttaggt ctctgtaatc tcgggcagct ttccgttatc
     2581 gttttaaccc aaagacacca tttcaatctg ctccatcgtc ctgcccagaa aacgctcgcc
     2641 gatggttctg aatttcaaag gaatatcgct gacgtaaaaa cggtagtcgg gattgttgtt
     2701 gccggtatcg agcaatcctt cctgtgcaag gacgcgtgcg gtttcttcgg ccgtcgtaat
     2761 cgcggaatcg accagcgcga cattgtgcgc ctccctgccg attaagggct tgagcaaggg
     2821 aaagtgcgtg cagcccaaca ccagcgtatc gatgccgtct gcaagcagtg gtttgaggta
     2881 ttcgcatacg gtcaggcggg taacttcgtg ttccaaccaa ccttcctcca ccaaagggac
     2941 gagcagcggc gcggcctgcg tgcggacgag cgtgtcgggg ttgtccctgt ggatggcgcg
     3001 cgcataagca ttgctgttga ctgtcgtatt ggtggcgatg atgccgattt tattgttgcg
     3061 cgtcgttgcc agcgcggctt tggcgccggc ggaaatcacg tccaaaacgg gcatattgcc
     3121 cgtcttttga cggattttcc gccccgcaac cgccgcaatc gtgttgcacg cgataaccat
     3181 cgccttgaca tcgtgtccca ataaaaaatc gacaatctgc atcgagaaat tttcgatggt
     3241 cgccttagat ttcgtcccgt aaggcacgcg cgccgtgtcg ccgaaataaa tgatgttctc
     3301 catcggcagc cgttccatca gcgcgcgcac attggtcaaa ccgccgattc ccgagtcaaa
     3361 aacgccgatg ggccgctgcc tgccgatatt ttccatcctt tttccaatcc gtcctaaaca
     3421 tacaatccgc atattgtaca cgcgccgctt tttcttgaca gccatgccgt ctgaaagcag
     3481 gatgcgtatt tggctgttat aatgtacggc aatgccgccg ccgggcgcac ggtttgccga
     3541 ccgcctgccc ctccgatccg ccacataaaa gttgtaaagc ccctatgtct gatttttccc
     3601 ccgttccccg attccttgcc gacgaagccg ccacactcga tttgggcgcg gcgtggtctt
     3661 cccgtttaaa cgcaccgctg gtcatttacc tcgaagggga tttgggtgcg ggcaaaacca
     3721 cgctgacgcg cggcatcctg cgcggattgg gtcaccaggg cgcagtcaaa agcccgacct
     3781 acgccatcgt cgaatcttat ccgctggaac gcttcgccct gcaccatttc gacctctacc
     3841 gcttctcatt ccccgaagaa tgggaagacg cggggcttga cgagctgttt gccgcaaaca
     3901 gcgtctgcct gatcgaatgg ccgcaacagg gaggcgaatt taccccgcct gccgacatca
     3961 ccgcaacatt gacacacggc ggcggcggca gaaaatgcct gctgaccgcc cataccgaac
     4021 gaggacgcga aagcctgccg ctatgaccaa actgacacga agacaaatca tccgccgcac
     4081 cgccggcaca ctgttcgccc taagccccat cgcatccgcc gttgccaaaa cggtacgcgc
     4141 cccgcagttt accgccgcac ggatatggcc gtcgcacacc tacacccgcc tgacgctgga
     4201 aagcaccgcc gcgctcaaat accagcactt cgcgctcgac aacccgggca ggttggtcgt
     4261 cgatatacaa aacgcaaaca tcaataccgt attgcacggg ctttcccaaa aagttatggc
     4321 ggacgacccc tttatccgca gcatacgcgc gggtcagaac acgccgacca ccgtccgcct
     4381 cgtcatcgac ctgaaacagc ccacccacgc acaagtcttc gcgcttccgc ccgtcggcgg
     4441 ctttaaggac cgcctcgtcg tcgacctcta tccgcacggg atggatgccg acgatccgat
     4501 gatggcgctg ctcaacggca gcctgaacaa aaccctgcgc ggctctccgg aagccgaccc
     4561 cgcccaaaac accacgcccc gacccgggcg gggcaaaaac gggcgcagac ccgtcatcat
     4621 gctcgatccg ggacacggcg gtgaagaccc cggcgccgtc agcccgggcg gtttgcagga
     4681 aaaacacgtc gtcctctcca tcgcccgcga aaccaaaaaa caattggaag ccttgggtta
     4741 taacgtcttt atgacgcgca acgaagacgt gttcatccca ttgggcgtgc gtgtcgccaa
     4801 agggcgcgca cggcgggcgg acgtattcgt ctccatccac gccgacgctt tcaccagccc
     4861 ctccgcgcgc ggaacggggg tttatatgct gaacaccaaa ggcgcgacca gctctgccgc
     4921 caaattcttg gaacaaaccc aaaataatgc cgacgcggtc ggcggcgtac cgaccagcgg
     4981 caaccgcaac gtcgataccg ccctgctcga catgacgcaa accgccacac tgcgcgacag
     5041 ccgcaaactc ggcaaactgg tgcttgaaga attgggcagg ctcaaccatc tgcacaaagg
     5101 cagggtggac gaagccaatt tcgccgtttt gcgcgcaccc gatatgccgt ctatcctggt
     5161 cgaaaccgcc ttcctgtcca atcctgccga agagaagctg ctgggcagcg aatccttccg
     5221 tcggcagtgc gcccaatcca ttgcctcggg tgtccaacgc tacatcaata catccgtatt
     5281 gaagcggggt tgattgaaaa ggcggtgttt caaaatatag ttgtcggatg aaggcaggca
     5341 taaacctact tccgccccaa accgcgtttt tatgatgaaa ggtatggata aattgagata
     5401 ccaacgggat tttttaaata tccgccccat ttttacagcc ggcgaacaag aatacctgac
     5461 cgaattgtcc gatcggcttc ctttatctgt tttaacggac tccgtgcgta atattgaaga
     5521 aatcggtatc gattttgtgt acagttctgc caaattggaa ggaaacactt ataatcaata
     5581 cgatacgcag gcattgctta aactcgggca aaccgcaggc gggaagttat attcggatgc
     5641 cgtaatgtta atcaacctga gggaaagtta ccggcacctg ctgtccggtt tggacagccc
     5701 caagccgttt gactggttgg atttcttaaa aaccacccac agcctgattt cggaaaactt
     5761 actggaaaaa ggctccggcg gcgtagtccg ccgcgacagc gttactataa gcgggacaga
     5821 ctacacccct ttatccaacc cgcaaagttt ggatacggaa ttgaaatggt tgcttcaaga
     5881 agcccccaaa atagaaaatc ctttcgaccg cgccgtttat ctccataata atttggcgta
     5941 tctccgatat tttaaggact gcaataaacg tactgccaga aactgtatga ccttgtcgct
     6001 gatgcgctcc ggtttttttc cttacgtatt ctcccccgac agttatcccg cctatgccga
     6061 ggctgtcgta gcctattacg aaaccggcga ttacggttta ttcaagaaat attttatttc
     6121 ggcatacgaa aataccgtaa ataagtacgg tccccagcct gatgtggata ttttccgaaa
     6181 tttctccatt taaaaagccg tctgaacatt tttcagacgg cttttttgta cactttcctc
     6241 aacggttcga tccctgcacc atatcgatca ggaacaggcg gcagtcgagg ttgccgttat
     6301 aaagcgggat tttccgcttg ggcagcaggc gcatgaattt gggcatttcc ctgtcgccgg
     6361 taaacatgcc gacccgccag ccggcatagt gttttttcaa ccacgtcccc aactgcggat
     6421 aaagtgcctg caaagcgcgg acttcctcaa ggcgcacgcc gtagggcgga ttggacacca
     6481 tgatgccgtt ttcgccgttc ggacggacgg actgcgcgcc ggcaacgctg aaggaaacga
     6541 tgtcgtccgc tccggcattg tgtgcgttgt ccaatgccgt ctgaacgatg cggcggccgt
     6601 tgtcgctgcc tgcaatcggg gcgcggacgg ggcgggtttg cgcttcggcg cggcggcgca
     6661 aatccgccca cagcgtttta tcgaaatttt gcagtttttc aaaaccgaag cggcgcatct
     6721 acccggcgcg cggccggcgg caatccaagc ggcttcgata gcaatcgtgc cgctgccgca
     6781 aaacgggtct tgaaacggct gcgtgccgtc gtagcctgcc gagagcagca gcccggcggc
     6841 aaggttttcg cgcagcgggg cttcgccggt atccaggcgg tagccgcgtt tgaacagggc
     6901 ttcgcccgaa gtgtcgatga agatttcgac attgcgttcg tccaaaaagg cgtggatgcg
     6961 gacatcgggc gcggctttgt ccacgctcgg acgtgcgtcg taaatgtcgc ggaaagcgtc
     7021 gcagacggca tctttgacgg tcaggccggc aaattggagg cttttgacgt tggcgcgttt
     7081 tgcctcgatt ttgactttga acgtctgctg caaggtaaac cagttgaacc agttgatgtt
     7141 tttggcaagt ttgtagatgt cgcgctcggt tcggtatgtg cctttggtca ggcgcagcag
     7201 gatgcggccg gcggtgcgcg agtgcaggtt ggcggcgtag gcctgttcca atccgccacg
     7261 gcaggaaacg ccgccgtcaa acgcccgaac ctcggtgcag ccgaggcttt cgagttcttg
     7321 cgataaaacg gtttccaagc cgcgcgggca ggtggcgaaa agtgtatata aagtattcat
     7381 agcgtgtcct ttccggacgg atgttccggc aatggcggat tgaaaggttt attcggcggt
     7441 aaatgccgtc tgaaatgtcc ggctgccgat tgtaatgtcc gccgtaaaat cgcgcctgcc
     7501 ttcgacacag acgggcaggc ggatgcgtgc tgcctgccaa ttccccgacg gttgccgctc
     7561 gaacatatag cggttgaaac ccatatccat atttttcata ctgaagctga tgctgacctg
     7621 ttccgtgccg gcgggcgcgt gttcgatata aatatcaaac ggttttttgg ttgaaacggc
     7681 ggcggcgcgg acacggcttc cgtccggcag cgtgcaaccc tcggtcaaat cgcattgcgc
     7741 cgccacggct tgcggctgct gcgcctgcca ccattgcaac aaaacgagct tgaaggcggc
     7801 aaaggcagtc agcagcagtg cggcaagcag taatttgcgg tttttattca tgattcttcc
     7861 ctattgtttc atcccttacc accgcgtgcg cccccaatcc gagccatagt tttccataac
     7921 gtgcaaccgt ctgtccgttt gctgcaagta cgagcggcac ggatacgccc tgccgcaggg
     7981 cttctgccgc ctcttgtgcc tgaacatcat cgcacacgcg ccacacgacc acatccgcgc
     8041 ccttttcctg cgcctgccgc cattgttcgc gggtctgcac catcatccac acgctgccgg
     8101 cgtgttcgga cctgtcctgc cattgtgccg cctccatcaa aacgttcgca ccgcttcccc
     8161 ctgccgaacc caaaggcagg acgcggtacg cgcccatact gttttcgccg tacaaacctg
     8221 ttttcaggct gccgtacaaa cggcgcggga cggacagcga acgcaaaagc gcaccgttgg
     8281 caggcagcat gggggcaacg gtaaaatcac ccgccttctg ccaagaccat tcctgccctt
     8341 cgcgcgattg cggtccgccc tcccattgat cggggtttac ccataagaat ttcaggcaga
     8401 cgcgggcgtg ttcgtaggaa tggattttgg tcagccaagg cgtggcggcg aggatgcgga
     8461 tgccgagttc ttctccaaac tcgcgttgca gggcttggaa gtcggtttcg cccgcctcga
     8521 ccttgccgcc ggcaaattcc caatatccgg catagggctt gccttcgggg cgcgagccga
     8581 gcaggtaatt gccgtctgaa tcgagcagga tgccggcaac gacgcggata agggggcggg
     8641 tatcttggat catggcgggt atgcggttgg tcggtcggaa tgcaaatgag ggaagggcaa
     8701 tgccgtcaac gggtttgccg aatttcagac ggcattattt ttcggcaacg gcaaacgcca
     8761 acacggtatc gccttcgtcc gccatactca ggctgacgcg gctgatgcct tgttcttcca
     8821 gccattcgga cagggcgggg ccgtagaaaa attcgggctt gcccaatgcg tcgtgcccga
     8881 tgccgatgtt gcagaaggaa accgcgcctc gtatgcccgt gccgacggct ttggcgaagg
     8941 cttctttggc ggcaaagcgt ttggcaaggt agttgacggg tttgcccgcc tgcggaaatt
     9001 caagcagctc ttcgggagtg agtatgcgcc ctgcaaacgc ctgtccgaat tttttgttta
     9061 agcggatgat gcgcttgagg gaaacgatgt ctgtaccgat gccgtaaatc atatttgcgc
     9121 tcctttttcg tgaaatgccg tctgaaacgg tttgcctgcc cttacggcag cagccttgcc
     9181 ctgaacatcg cctccttcat ctggcgcacg gcttcgggaa gtccgaggaa gagggcttgg
     9241 gcaatcagcg aatgcccgat gttcagttcg cggatggcga ggatttgggc gacgggggta
     9301 acgttgtgta tggtcagtcc gtgtccggcg ttgacgacca agcccaaatc gccggcgaaa
     9361 tgcgcgccgt tttgcagacg ctcgaactgc ctgatttgtt cggcgtggct gtgtgcgtcg
     9421 gcatatgcgc cggtgtgcag ctcgacaacg ggcgcgccga catcataggc ggcttggatt
     9481 tgcctgtcgt cggcatcgat aaacaaggac acgcgtatgc ccgcgtcggt caggattttg
     9541 gcgaattcgg cgattttttc ctgttgcgcc aatacgtcca aaccgccttc ggtcgtgatt
     9601 tcctgccgtt tttcaggcac gatgcacacg tcttccggca tcactttaag cgcgttttcg
     9661 agcatttctt ccgtcaacgc catttcaagg ttcaggcgcg tgcggatggc gtttttgacg
     9721 gcaaatacat ccgcgtcttt gatgtggcgg cggtcttcgc gcaggtgcat ggtaatcagg
     9781 tctgcaccgt gcgtttcggc aaccagtgcc gcctccacgg ggctgggata agtcgtgccg
     9841 cgcgcgttgc ggacggtggc gatgtggtcg atgttgacac ccagaagcat aatgtttcct
     9901 tttctcttcg ttttcctgtt tgacggcatg gtgcgggcgc agctacgcgg tattgcggtc
     9961 gaactgcctt atctgttcca acacctgccg cgatttcaag ccttcgggaa gcagcgtgcc
    10021 gattagcagc cgtgtgattt tcaatgcctg ttgcaggctt tcggcggtgc ggaaactgcc
    10081 ttcgcgcaaa tcgatcaggc tctgcccttc gacaattgcg ccggcttcgt gcgaaagcgc
    10141 gcctgtgtcc cggcatacgg gcatgaccgc ctcttcgggc atcagccggt aggttttgtc
    10201 cgccaaaata tcgccgcccg tgccgtcggc gtgcaaatcg ggggcaacgc ccaaggcatt
    10261 gagcagcttc cattcgaagc ggcgcaaatc ggcaatatgg ttggcttcac ggcacactgc
    10321 ctccatcact tttgccaacg cgtcgtacag ttcggacatc gggtcttcgc gcgcggtcag
    10381 tttcaacacc agctcgttca catacagacc gctgaacaac gccctgccct gcggctgccg
    10441 ccatccgccc atccattcgg cgcggtgcag ggttttgagt tcctgactgc cgtaccacga
    10501 cacgctggca gggacgaacg gaaccaatac gccgcgcagc tcgctctgcc gtttgcgcgc
    10561 gctgcgcgcc agcaaagcca cacgcccgta acggcggctg aatgcttcaa cccgcaggct
    10621 gctttcgcgc caaggcgaag atgccagcat aaaaacgggt tcgtggttga ctcggtgttc
    10681 ggacataatg cctgaaatgc cgtctgaacg ctgccgcgac tacaaaacgg cagtcggata
    10741 cgaaccgatg gctttgacaa acgaagcgcg ttcgcccaag cgttccaatg ccgtctgaat
    10801 ctgcgcgtcc cggcggtgtc cttcgatgtc gatgaagaac aggtattccc acaaaacgga
    10861 tttgctcgga cggctctcaa acttggtcat ggaaataccc gattcggtca gcggttgcag
    10921 cagcgaggca accgcgccgg cgcggttggg cgcggaaacg gccagcgagg ttttgtcgct
    10981 gccgcttgca ccggtttcgt gatgtcccat gaccagaaag cgcgtggtgt tgttcggttc
    11041 gtcttcgatg cactcggcaa ccatatcgag tccgtaaatt tccgccgccg tgcgtccggc
    11101 gatggcggca accgtaccgt cgtccgattc ggcaaccagc cttgcggctt cggcattgct
    11161 ggacacggca atccgttcgg cgttgggcag acgcctgccc aaccagtcgt tgcactgcgc
    11221 caacgcctgc gcgtgcgaaa atactttggc gataccctcg gtgctgccgt tgtttttgcg
    11281 caacaggttg tggcggatgc gcaaaacgac ttcgccgcac gcctgcaacg cggtaacggc
    11341 aagcaaatcc aatgtgcgcc cgaccgagcc ttcggtcgag ttttccacgg gcgcgaccag
    11401 ataatccgcc tgacgggttt caacctgttt gaagcagtcg tctatggtcg gacacgccat
    11461 tgtatgcgcg gcatgaccga aatgcttgat tgccgcctgc tgggtaaacg tgccctgcgg
    11521 ccccagatag gcgatggtca gcggacgttc gacggcgagg cactcgctca tcacttcccg
    11581 aaacaggcgt gctaccgatt cgtcgggcag cgggcctttg ttcaaatcct gaatgcggcg
    11641 caacacggca acctcgcgtt cggggcggta caccgcgccc gtgcctttca gctcgccgat
    11701 ggcgtgggca tgttgcgcgc gttcgttgag caggcgcagg atttcggcat cgatggtgtc
    11761 gatggcgttg cggtgcggga tgaggagttc gtcgatagtt tgggacataa tttcgcctga
    11821 acggtaattt gccgtgcatt ctaacatcaa atgccgtctg aagcggtttc agacggcatt
    11881 ttccgttaaa ggtattcgct ttgcccgttt tctttccacg cacgatgcga gcagagccac
    11941 aaaagcgcaa tgccgcaaga ggcggacgcg gtcatggttg ccgccatcac ggttgccgaa
    12001 ccgtcgtgca ggacggttgc cgccatgccc acgcccgcgc cgattaagga ccggaataca
    12061 cccgacacgg cgttcgcgct gccgccctct tctttgaaat aagacataaa gcatgcctgc
    12121 gtgtccgcac cgaccaggcc ctgcgtaccg acggaaaaca tcacgcacgc aaccggcagc
    12181 caaaacgggg gcaacccgaa aaacagcacg gcggcgagtt gggacgggtt ggcggcaaat
    12241 tggacgacaa tcccccgcag caggatgctt tgcggatgcg cgccggtttt aagccgccac
    12301 gcggtaacgc ggctgaaaaa catcatcgtg atgatgttga gtgcaaacac ccatgcgtac
    12361 cggtgcggcg taacgtggta gagctgccgg tacacgaagg aagattcggt cagaaaggcg
    12421 aacatcgaac cgaagctgaa tgcctgaaaa aacagataac ccatcgcggc acgggttttc
    12481 aatacgcgct tgaaccgccc cgccaccagc ccgaacacat ccctgccgat tttgccgccg
    12541 acggcgggat tgggcaggaa atactgtacc aaaccgggca gcaccggcga atacgccgcc
    12601 aagaaaacga aaatcgcccg ccatccgccc aatccctgca acaatgcgcc gaccatgggt
    12661 ccggccagcg gcacaaccat cagaatgatg ccgataaggg caaacatctg cgcggctttg
    12721 cgtccggaat aataatcgcg caccatcgca ccgacgatga ctacagccat gcctgcgccg
    12781 aacgcctgta ccgcacgcag gttaaggagc tgttcggtac tcgaagcaaa tacgatggcg
    12841 gcaacggcaa ggcaatatac aatcaaaccg gtcagggcga cgggtttgcg ccctttgatg
    12901 tcggacaccg cgccgccggc cacttgcccg aacgccgtgc cgaacataaa caaactcaga
    12961 ctctattcga tacggtggat atccgcgttc agcggctgcg ccatttcggg aatcgcgggc
    13021 aggtaggcat cggtggaaaa cggcatcagc gcgaccagta ccgccatcaa aaccgccatc
    13081 agttttccgc tcatcggagg aaggggcgga gacatggttt ttccgttcaa atcaaataaa
    13141 gaaaccgtcg caaacgacgg tcggtacggg cgcaagtcta acatatcgga cggcggggca
    13201 aaaccgaagg atatgccgcc cgaaaccgct tcagacggca ttgccgtttt gcgcgtccct
    13261 gcgcgacagc cggccggcgg cgaaagtggc ggcgatgacg acaaacacgc ccaaggcgga
    13321 cacgggcgat aaatgttcgc ccaaaatcaa aaccgccaac agcacgccga cgacgggttc
    13381 gagcgaaatc aacagtcccg acgcgttggc aggaacacgg ctcatcccct tgttccacag
    13441 ccaataggcg taccagccgc accccaaacc caaatacaac agcgacaata ccatcccgac
    13501 gctccagtcc acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga
    13561 tgcggcggca atggaaacag atgtgaatgc cggtgcgccg atgcgggcaa tcagcctttg
    13621 cgtcggacgc atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa
    13681 ccagccgact tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa
    13741 tgccgccgcg ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc
    13801 gacaaacacc atcagcagcg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt
    13861 gtatttcaac ccgacaaact gaagcagcag ggtcagcaca tagttgacga acgacacaat
    13921 cagcaacggc ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag
    13981 tgcaggcagc gcggcaatca gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc
    14041 atagacatat ttggcggcaa taaacgagct gccccagata atcagggcaa ggatttggta
    14101 aaacatagtg agtggtaaag tcagatggtc atacaaaagt atagcggatt aacaaaaatc
    14161 aggacaagaa ggaatgcccg aaccgtcatt cccgccactt ttcgtcattc ccgcgaaagc
    14221 gggaatctgg aatctcggac tttcagataa tctttgaata ttgccgttgt tccaaggtcc
    14281 ggactcccgc ccgcgcgcaa gtttccgaag ccatcctttt ggccgaaggt caaaaatcag
    14341 ccgttaccga gtattacctg aatcacggca tatggccgga aaacaacgct tctgccggcg
    14401 tggcatcccc cccctccgac aaaatcaaag gcaaatatgt tgaaagcgtt acggtcgcaa
    14461 aaggcgtcgt taccgccaaa atggcttcaa gcaacgtaaa caaagaaatc caaggcaaaa
    14521 gactctccct gtgggccaag cgtgaaaacg gttcggtaaa atggttctgc ggacagccgg
    14581 ttaagcgcgc cgacaa
//
LOCUS       DAXDZG010000058        14529 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXDZG010000058 DAXDZG010000000
VERSION     DAXDZG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.058"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(323..4492)
                     /locus_tag="ACFB78_001540"
     CDS             complement(323..4492)
                     /locus_tag="ACFB78_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC1269507.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4547..6394)
                     /locus_tag="ACFB78_001541"
     CDS             complement(4547..6394)
                     /locus_tag="ACFB78_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC1269508.1"
                     /translation="MHDTRTMMIKPTSLLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNIEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6765..7994
                     /gene="sstT"
                     /locus_tag="ACFB78_001542"
     CDS             6765..7994
                     /gene="sstT"
                     /locus_tag="ACFB78_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC1269509.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8145..9425
                     /locus_tag="ACFB78_001543"
     CDS             8145..9425
                     /locus_tag="ACFB78_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC1269510.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFADKKDRD"
     gene            9425..9628
                     /locus_tag="ACFB78_001544"
     CDS             9425..9628
                     /locus_tag="ACFB78_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC1269511.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9688..9936
                     /locus_tag="ACFB78_001545"
     CDS             9688..9936
                     /locus_tag="ACFB78_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269512.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9929..10144)
                     /locus_tag="ACFB78_001546"
     CDS             complement(9929..10144)
                     /locus_tag="ACFB78_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269513.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10137..10352)
                     /locus_tag="ACFB78_001547"
     CDS             complement(10137..10352)
                     /locus_tag="ACFB78_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC1269514.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10555..11898)
                     /gene="argG"
                     /locus_tag="ACFB78_001548"
     CDS             complement(10555..11898)
                     /gene="argG"
                     /locus_tag="ACFB78_001548"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC1269515.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12023..12823
                     /locus_tag="ACFB78_001549"
     CDS             12023..12823
                     /locus_tag="ACFB78_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC1269516.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            12863..13849
                     /locus_tag="ACFB78_001550"
     CDS             12863..13849
                     /locus_tag="ACFB78_001550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC1269517.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
BASE COUNT         3033 a         3729 c         4393 g         3374 t
ORIGIN      
        1 tttcttaacc atcccttcca atagccgtgc gaaggttttt ttatatcagt gggaaatgca
       61 atattctatt gtttttattg tagaatttta aaaacagatt gttgtgttcc gcgtttttgc
      121 cggtttggaa gcggtggggc gcatcagcct tttataaagg catatcggaa gcctgtataa
      181 ggtttttgaa catatcgatc ctgttccctt gcaggcggac ggcagcggcg gatggcgaaa
      241 ggcgggtttt caaatgtccg gtttggcggc gcgtctttca gacggcatgg cggcggagcg
      301 tgccgcgccg tctgaaaacc gtttatttcc ctttgccgtt tccggcggag tcttttttgt
      361 ccgaaccgaa gaggcggtcg aaacgtatgg tgtatgtcag ctcgccgccc gacgaacggc
      421 tgccgatacg ggcaaccgcc tgtatggcgc gggtcagccg gtaaatcagt ttgacggact
      481 gttccgcgct ggagatgccg tattcgtagc cgatgtagag tttgccggtc agttgtttgc
      541 cgacggtcag cacctgttcg gcggggttga gttcgccggt ttgcgcgttg cggctgcgct
      601 tgctggtaaa gcccaaatca tccaccagcc cgatgcggtc gttgatttgc ccggcaagca
      661 gcgcgcctgc ggctgcggac agggcggcat tgtcgccgct gctgccgctg ccggcgcggt
      721 tgaggatgag ccaggagagc ttgtcttttt cactcatcgg ttcgtttgcc gtcagcgtaa
      781 tgcgcgggct gttgaggctg cccaatattt ccacgcccgc accgacgggg gaaaggcggc
      841 gttcggcgcg gatgttgagg ttggggtcgt tgagcgggcc gacaaaggag actgtgcctt
      901 tggtaatgtc taaatcctgc ccgtatgctt tgtaacgccc tttgatgacg cggaccgtgc
      961 ccaccccacg cacatttccg cccggttgcg cggtcagggt cagtttgccg cctatggtaa
     1021 cgtccgcgcc gtagccggag aagcggatgc cgtcattgag gtctaaagtc aggttcatat
     1081 tgacggggag cgatgccgcc gcctctttct tgacttcgcc caatacgacg acatcgtcgc
     1141 cgacggacgg catcgaggat ttttgcgaac cgaacagccc ctgatcagtt ttaatcatac
     1201 cggtaacgga tatgcctttt tgcggcgaat agcgcaggcg ggtgttgccg gaaaccgtca
     1261 ggcggcggtt ggggcgggac aggatgcggt atttgtcgaa caccgcgccg atatcgacat
     1321 cgggcacgct gttttccatg ctgaccgtgc cggagagttc cgccgtccct tcgtgccgga
     1381 atttcaggct gtcgattacc catttcctgc ctgcaatatg cgaacgcagc gagccgttgt
     1441 ccaagatgat gccttgggtt tggttgcggt aatagagctt gtcgccgttg atactgccgc
     1501 ccaagtgcgg gtcggcgatg ctgccgccga gggttacggc ggcattcagg ctgcctttga
     1561 cggtttgtcc gaccggtagg aagttgcgga atacttcggc atcggcaacg gtcaggttga
     1621 gcctgccgcc caaaggtgcg gtatcgaagg agcggctttg cccgacggtg atattgccgt
     1681 tgattttccc gtagttgctg ctaccgttga cggcggcatt gacggacgga gagcttaccc
     1741 gtccgccgat ttgcgcggag gcattcaggc tgccggtaat gttttgcgcg gcggcgggca
     1801 gaaagggctt caatgcgccc aagtcgggaa gggaggctgt aatcctgccg ccgagcggtg
     1861 tatttgccat attgccgccg aaggcgttgc cgatgcccaa atcggcgtta atccgtccga
     1921 aacgcgcgcc gccgtcaagc aggattccga tgcggtcgtt ttgaaagcgc gttttcaggg
     1981 aaaatgcgtt caaacccaaa gcctgcccgc cgggcaatac ggcatcgccg ctttgccggc
     2041 tgatattgag gtagccgcgc gcgttgtgcc cgtaggcgac atcccagtcg ccgtttaaaa
     2101 ccagattgtg ttcgaagggc ggtttgaaga aattgtgcaa ctcggcgatg tgcaggccgc
     2161 gtgcgccgcc ttttgccgat atgccggttt tcctgtccca agaaaagtgt tgcaggttga
     2221 ggctgccgcc cattgcctgc caatttgccg cacttgccgc cacgtgttcc gcaccggctt
     2281 cgagcgtcat acggttttgc agcttgaggt tgaatgcgcc gccgatgtcg aggatgccga
     2341 tgctgccttt ccatcgggta agttccctgt tgatgccgcc tgaagcgtcc aaatcgagtt
     2401 tgaacggttt gccgtccagc gtcatggcgg cgtgtgtgcg gatgcggtgc tgcgcgcccg
     2461 taccttccag cgtcaggccg gcggtatcga caaccgccgc gccgcccgac agggaaaggc
     2521 ggccgccctt gatatcggcg cgcatcgggc ggcttgtgtc gggcgagcct ttgagggtaa
     2581 aatcgagcga acggatgtct gccgctttgc cgatgtgtaa gttgcgcgcc gtgccggaaa
     2641 ggtcggtttc aaaggttcgg atgccgccgt ccaaatcgcc ggaaaggtgt ccgcgtacat
     2701 ttaaagaccc cgcgagtccg aaaccgaaac gggataaatc gggtgcggtg atattgaggt
     2761 taagccggtc gccttttttg ccgaagccgc cgtctgtttt gacgatgttc cgccccaacc
     2821 gcaaatcgac ggcggcacgc ggaaggtggc gggactcgta aacaatgtcg gcactgccgg
     2881 caatcggcac gccgttgaac gtaccgggca aaaaacgcat tttgcccgta aatttctctt
     2941 ttgccagttc accggcaaga tgaatcgaac cgttgatatt gcctgccgga aattgcggat
     3001 cgatgcgtga agggtcgaat gcgcgggaac ggatgtccag cttgagcagg cggtctttaa
     3061 acagctcgag atagccttgc gcggtcaggc tgccttcccc ggcggagatg ttgacggtgt
     3121 cgaacaccag tttccgctgt tcgtttgcgg ggtcgcttgc gatggcgagg ctgccgtccg
     3181 tgcgtgccgt gccggtgccg agttgccaag agattttggg cgaggcggtc gtgccgccga
     3241 tgccgatgct gccgtccaac ctgcctttga acgcggtttg cagcacgtct tccgcgccga
     3301 cggagttgat gcctatattt aaatcaagga tgtctttttc ggtgtcgatt ttgcccgaca
     3361 gcctgatgcc gccccgtccg agcagggcgg cggacgtatt gccgatatgc accgtgccgt
     3421 cctgccggat gacaaagccg cccaaaacct gacggacggg gatgccgttg cggtcggcaa
     3481 agccggcttt ggtgttttcc aaatcgagcg agccttccag cgcgatgccg tctgaaaacg
     3541 acgggatggc ggtcaggtcg aaattcagcc cggcatcggg cagggaaggc acgaaggcgg
     3601 acggattgat gttgaatcct ttgaccagta cttcttccaa tgttttatcc aatgattcgg
     3661 caaacgggtg gatgacggat tttcccgaga ggcggatatt gccgccgtcg atcgtcagtt
     3721 cggcgcgcac atccttcagg ctgccgctca gccgcgccgt actgtgtatg gtttcgcctt
     3781 cgaatccgcc tttggtgtaa atggcggtat cgagggcaaa cggttttttc aagccgaccg
     3841 aggctgaccc cgacgaactg ctccacggcg tgtcggcggc cttcaggtcg aggcggtgcc
     3901 ctttacggtc gtaacggtat gccgcgttga ggcgttcgag atagacagtt tgtttgtcaa
     3961 aggttttgcc catgctgatt ttgcccgtct cgaagcggtc gagatagacg gtggcgggca
     4021 ggtctatgct gtcgggcagg ccttgaggcg ggcgttcttc tttaggcgga gtcggtttgg
     4081 ttacgatggc gatgtcgccg gcggagatgt cggtgatgtg caggctgcgg cgcatcagtt
     4141 cggacggttt ccacgcgaag cggaagcggc tgattttaag gtctgccccc tcggtttcta
     4201 tcgaccagtt gtcgccgtcg aagccgtcga gcagtgtgcc tttgaggttt tgggaggaaa
     4261 tgtttacgcc gaaccaggac gggatttggt acagcccgaa gcgcaaacct gcttccgtac
     4321 cggcgatcca gccgaggaaa catactgcca aaatcaggac agacagcagt gccgccgaca
     4381 gcttcagcag cgggcggcgt ttttttgccg gcggggcggg gcggtgttca gacggcattt
     4441 tgcgcgtgcc gttttcggtc ggatcggtat ctgtcggtgc ggtatcggtc ataatgtctg
     4501 tttcaaaatg gtttgctgca atgccgtctg aagcggccgt atcggtttag aaacgcgttc
     4561 ccaagctgat gtgccagcgg atttttttgt cgctgtgtcc gtaggcgatg tcgaaggaaa
     4621 acggcgcgag cgggctgaac cagcgcacgc ccagtcccga accgtgtttc agcttcatac
     4681 gtttgaaatt ggcggcggca tcgcccatat cgtggaatac cgcgccggac agggtgcgcg
     4741 taaacggcag ttggtattcc aagctgccca ccaagagggc gcgttcgggc aggaccgatc
     4801 cgttcggacc ggcaagcccg atgctgtcaa gttcgtaacc gcgcacggaa gacgcgccgc
     4861 cgctgcggaa catcagcccc gaggggacat cggcattgtc gcgtgcaacg gtgtaacccg
     4921 cttgtccgcg tatgatgaac gtgccgagtt ttttgttttc gggcgtgaag aaataacctg
     4981 cgcgggcaga ggtgcggatt agcgcggtgg aggacaggaa tgtgcccaaa gtcgtcccga
     5041 ttttgccgtc gaggtaatgg ccgttttcgg ggtgcagcac gttgttgagc agctggcgtt
     5101 tccaagaggc ggtcagcatc gtggcgtggc tgttgcccaa atcgacatcc gagccgggga
     5161 ttttccggcc ttctgcgaga aattccgccc ccagcctggc atcgatgccc gcgcggtcgc
     5221 gcacatacca gatgccgccg gagaaggcgc gtttttcaat gttttgggtg gtcgaacggt
     5281 tgtaggaaac gttgcttgtc cagtagttgc cctgatagtt gcgcggctgg ctgatgccgg
     5341 cggcaagcgt ggtttcgtat ttgtccatat cccagacgac cgagccgata tagcctttgt
     5401 tgaagaggtt gtaatagtcg taggcgattt tgccgcccaa accgtattcc gaatcgaggc
     5461 ggatgccggt ttcgagtttg tggcgtttga cctcggttac gctgactttg acggggacgc
     5521 ggtcgccttg gaggcggtcg aagtcggctt gtacggacgc gccggaataa tgtccgtttt
     5581 gttcgagcgc ctgttggaag tcgagcagca ggtcgaggtc gtagggcgtg cccggttgga
     5641 agcgcgccag gccggagacg gtttgttcgg ggtaacgctg tgtgccggtg atttcaaagt
     5701 cgccgaaggc gatggggcgg ccgctgtcca cgacgacgtt caaatcggcg gtggcggtat
     5761 cggggttgac ggccgcccgg gtgttgccga gcttggcaag cgggtagcct ttgcgcgtta
     5821 ccgcgccgag gacggaagtt ttgctgtttt cccaactgtc ctgatcgaaa tcgctgccta
     5881 ccggctgctg ccagttttcc agcgcgttgc ggtagtattc ggcgaggttg ccgtctgaaa
     5941 ggatgtcgcc gaggatggcg acgccgacgt tggcgatttt ggtgcgcggg cccggtgtga
     6001 tgtgcaccgt ataagctccg tctttttccg tcaggctgac cttgctgctg aaatagcctt
     6061 tgctgcggag cattgtttta acgttgtccg gtgcttcttc ggcaaggaat cccgtctgtt
     6121 ccttatccaa aacctcttcc tgctgctgcg tgatgagcgg caggtgttct tcgaccatat
     6181 ctttgatttc actgtcctgc gtgtcgatgc ggacggggaa tttgggtttt aatttgactg
     6241 attcggtgtc ggggcttttg cttttgaaca atgcgaaacc cgccgccttg ttttcggaaa
     6301 ggtcggcggc aggcgcgtat gcgtgcggaa agaaaaataa agccggcagg agcagggagg
     6361 tcggtttgat catcatggta cgggtgtcgt gcatcggtgt ggcggatttc gattctaaca
     6421 ctgttcggac ggggcgggac gcttcagacg gcataccgcc gccggtattt cggaggttgc
     6481 ggcccgccgg aaatgtatct gcttgtttta aggcattttg tttccaatcc gcatatggaa
     6541 aacaggctgc gtttcggaat agtgtcgaaa aataggcagg attaaataaa tttattctcg
     6601 ttcaataaat taaactgttg cgccgaatga taaaacaaag cctgtccctg catagggcaa
     6661 atcaacgcac ctcaaaacac agcttgaaaa ataaaaaaac acagagaatg agttttcttt
     6721 taaaatacaa cattttttaa catatcacaa cataaggaag aattatggcg ttcggcaaat
     6781 ccttatttca tgcgatcggc cgcgtcagcc tggtcaggca gattgccgcc ggtttggcgt
     6841 tgggcatcgt aatcggttcg gtttcaccgc aactgggctt ggcggcaggc ttgttcggca
     6901 gcctgtttgt cggcgcgctc aaagcggtcg cgccggtttt ggtatttatt ttggtggcgg
     6961 ccacaatcgc gcagcaccaa aaaggcaaca aggcgcatat caggccgatt atcgtccttt
     7021 acctcatcgg cacgttttcc gcagccctga ccgccgtcat cgccggtatg gttttcccga
     7081 cgcacattgt tttggcgggc gcgggcgatg tgtccgccgc gccgccttcc ggcattgtgg
     7141 aagtgttgaa atcgctgctg atgaacctgg tcgccaaccc gattaacgcg attgccaatg
     7201 ccaactatat cggcattttg gcttgggctt tggttttggg cgcggcgttg cggaatcacg
     7261 gttcggacgt tacgcggcag gtcgttgccg atttggcgga agcggtttcc accgtcgtga
     7321 aatggattat ccgttttgcc cctttgggta ttttcgggct ggtgtcgtcc acaatcgcgg
     7381 agacgggttt cggcgcgctg gcgggttatg cgaagctgct tgcggtgctg ttgggctgta
     7441 tggcgtttat cgcgctggcg gtcaatcccg ccatcgtgtg gtggaaaatc cgccgcaacc
     7501 cttatccgct ggtgtttacc tgcctgcgcg aaagcggcgt gtatgccttc tttacccgtt
     7561 cttccgccgc caatatcccc gtgaatatgg ctttggcgaa aaagctgggg ctgcacgaag
     7621 acacttattc gatttccatc ccgttggggg cgaccgtcaa tatgggcggc gcggcgatta
     7681 cgattaccgt tttggctatg gcggcggcgc acacccaagg catacaggtt gattttgcca
     7741 ccgcgctgct gttgagcctg gtggcgacgg tcagcgcgtg cggcgcgtcc ggcgtggccg
     7801 gcggttcgct gctgctgatt ccgctggcgt gcagcctgtt cggcatcagc aacgatgtcg
     7861 ccatgcaggt ggtcgccgtc ggcttcatca tcggcgtgat tcaggattcg gcggaaacgg
     7921 cgttgaattc ttcaaccgac gttttgttta ccgctgccgc cgatttgggc cgacagagaa
     7981 accgtgcgga ataaaccaag ctatgccgtc tgaagcccgt ttggcgttca gacggcatat
     8041 ttttacaatt ttatgggcat atggcggaaa caataccggc tgccttgaat atgtttcaac
     8101 acacaggtcg atacataaag cgtcgcccta tgaaggagat gatgatgttt aaacgcagtg
     8161 tgattgcaat ggcttgtatt tttccccttt cagcctgtgg gggcggcggt ggcggatcgc
     8221 ccgatgtcaa gtcggcggac acgccgtcaa aaccggccgc ccccgttgtt gctgaaaatg
     8281 ccggggaagg ggtgctgccg aaagaaaaga aagatgagga ggcagcgggc ggtgcgccgc
     8341 aagccgatac gcaggacgca accgccggag aaggcagcca agatatggcg gcagtttcgg
     8401 cagaaaatac aggcaatggc ggtgcggcaa caacggacaa ccccaaaaat gaagacgcgg
     8461 gggcgcaaaa tgatatgccg caaaatgccg ccgaatccgc aaatcaaaca gggaacaacc
     8521 aacccgccgg ttcttcagat tccgcccccg cgtcaaaccc tgcccctgcg aatggcggta
     8581 gcgattttgg aaggacgaac gtgggcaatt ctgttgtgat tgacggaccg tcgcaaaata
     8641 taacgttgac ccactgtaaa ggcgatcctt gtaatggtga taatttattg gatgaagaag
     8701 caccgccaaa atcagaattt gaatcgttaa gtgatgaaga gaaaattaag aaatataaaa
     8761 aagatggaga aaagtttact ggtttggttg ctataaaagt tgaaaataat gggttgaata
     8821 aatataccat tatttatcaa gctcagccta ctcgttctgc acggtcgagg aggtcgcttc
     8881 cggccgagat tccgctgatt cccgtcaatc aggccgatac gctgattgtg gatggggaag
     8941 cggtcagcct gacggggcat tccggcaata tcttcgcgcc cgaagggaat taccggtatc
     9001 tgacttacgg ggcggaaaaa ttgcccggcg gatcgtatgc cctccgtgtg caaggcgaac
     9061 cggcaaaagg cgaaatgctt gttggcacgg ccgtgtacaa cggcgaagtg ctgcatttcc
     9121 atatggaaaa cggccgtccg tacccgtccg gaggcaggtt tgccgcaaaa gtcgatttcg
     9181 gcagcaaatc tgtggacggc attatcgaca gcggcgatga tttgcatatg ggtacgcaaa
     9241 aattcaaagc cgccatcgat ggaaacggct ttaaggggac ttggacggaa aatggcggcg
     9301 gggatgtttc cggaaggttt tacggcccgg ccggcgagga agtggcggga aaatacagct
     9361 atcgcccgac agatgctgaa aagggcggat tcggcgtgtt tgccgacaaa aaagatcggg
     9421 attgatgttg atgccgcccg aagcgcaggt attcattacg gcgggacgac gcgttcaaat
     9481 catcaaacaa gcccccgaaa aaggcggcat cccaaaatcc ggcactgaaa ttgccgcgcg
     9541 tatcggcggc attccgcagc ctgccgggga aaccgtcggc agcagccgga atgtcgggta
     9601 tttccgcccg cctttttcgg gctgttgaaa tatgaaaata aagcataggc cgtctgaatg
     9661 ttcagacggc ctgttttttt aacggctgtg tttcaacact ccgccggttc caaccggcag
     9721 cagcagggag ataatgcctt gaaccccgct cttcggggcg gcagggttcg ggccggtttg
     9781 gggaaaggca tcgcctttcc gaatcggggc ggtgcaacgg ggtgcgcctt atgtgccgaa
     9841 agggtttgcg gcaacggtat gccgtctgaa gcgggtgcgt tcagacggca ttgtgatgtt
     9901 tggggcgcgg gtccggttca aatccgcatc agttgataaa cccgttgtct ttcattttct
     9961 tttcggaaaa atactggccg cataccagca gggcggcgag gatgatgaca aacagccctg
    10021 ccatagtcag tgtgattttg aagaacaatt cggcttcgag aaaagaaaac caaagttgca
    10081 caatcagcag cagcacccag ccggcaaaca gtccgagaca ggcataaacg ccgagcttaa
    10141 acacggtccc gctccaaaat gacgacgtag gcttcgcgtt tgaaaaacag cagcatacgg
    10201 cgcaaatctt tttccatcgt tacgacccgc cagccttcgg gggtttgttt gttgaggaac
    10261 gcgctgaaat tgacggggtt gacctttgcc gcgccgaaaa acaggctgga caacaggctt
    10321 tcctgataaa tgacgacttt gtattctttc atcgtatttc cttttcggtt gaaaccccgc
    10381 cctttggggc ggtaggatca gactttattt gggagggaac cccttccaaa tcaggacgat
    10441 acataggtcg gtgctttatg tatcgtcctg tgtgttgaaa cataggattc ctttaatcaa
    10501 gggattgcct tttcaggcaa tcccttaaat tgcagcgatg caatgcggcg aaatttattg
    10561 cttattgccc aactgcggca ataccgagcc ttcgcccagc gagagcaaac cgctttgcga
    10621 ataaataccc agtttggcgc gggtgtcggt gatgtcgagg ttgcgcatcg tgagctgtcc
    10681 gatgcggtcg agcggtgtga acgccgcacc ttcgactttt tccatactca ggcgttcggg
    10741 ttggtaggtc aggttgggcg attcggtgtt cagaatcgag tagtcgttgc cgcgccgcag
    10801 ttcgagggta acttcgccgg taacggcttt ggcgacccag cgttgggcgg tttcgcgcaa
    10861 catcaaggct tggctgtcga accagcggcc ttggtagagc aaacgtccga ggcgcaggcc
    10921 gttgatgcgg tattgttcga tggtgtcttc gttgtggatg ccggtaacca agcgttcgta
    10981 ggcgatgtgg aacaatgcca tgcccggggc ttcgtagatg ccgcgcgatt tggcttcgat
    11041 gatgcggttt tcgatttggt cgctcatacc caagccgtgg cggccgccga tgcggttggc
    11101 ttcgaggaag agttcgacgg ggtcggcgta ttctttgccg ttcaatgcaa ccggcacgcc
    11161 ttcttcaaag cgcacgctga cttcttcggg ttcaatttcg acgttttcgt cccagaaggc
    11221 aacgcccatg atgggcttaa cgattttgat gcccgagttc aaaaattcca agtctttggc
    11281 ttcgtgggtc gcgccgagca tattggaatc ggtggagtag gctttttcaa ccgacatttt
    11341 gtagttgaag ccgttggcaa tcagaaattc gctcatttcg tgacggccgc cgagttcgtc
    11401 gataaattgc tggtcgagcc agggtttgta gattttcagc gcgggattgg tgagcaagcc
    11461 gtagcggtag aaacgctcga tgtcgttgcc tttgtaggtg ctgccgtcgc cccagatatt
    11521 cacatcgtct tctttcatgg cggaaacgag catggtgccg gtcacggcgc ggcccagagg
    11581 cgtggtgttg aaataggcga tgccgccggt ggaaacgtgg aacgcgccgc attggatggc
    11641 ggcgatgcct tcgtgtgcca gttgtgcgcg gcagtcgatc aggcgggcgt tttccgcacc
    11701 gtattccatt gcttttttgg gaatggcgtt gtaatcgtct tcgtcgggct ggccaaggtt
    11761 ggcagtgtag gcataaggca gcgcgccttt gagtttcatc cacaacagcg cggcagaggt
    11821 atcaagaccg ccggagaagg cgatgccgac tttctgaccg acggggaggt tttgtaaaat
    11881 agtatggttt tggttcatga aaaaatcctc gatagcttga aaaatcaaag ccattatctt
    11941 ataaatagaa aatattgtca gccatcatgc cgtctgaacg ggtattccgc cgtttcaggc
    12001 ggtatttaaa accgaaaaca ttatgaacgc gttcaacctc atcatcatcg gagacgaaat
    12061 cttacacggc agccgccaag acaaacattt cgcctttttt aaatccctgc tcgaatccaa
    12121 agggctgaag ctcaatcagg tgcaatatct gcccgacgaa cccgatttgc tggtcaggca
    12181 actgcgccgc agcttttcag acggcatacc gacgttcgtt accggcggca tcggcgccac
    12241 gcccgacgac cgcacgcgcc aaaccgcagc ggctgctttg gatttgcccg tcgtccgcca
    12301 tcctgaagcc gccaagttta tcgaaggcat aacccggaaa cgcggcaagc cgctcgattc
    12361 gcccgaacac gcccaacgcc tgaaaatggc ggatttcccc gagggcgcag agcttgtgcc
    12421 caacccgttc aacaacatcg ccggattttc catccgcgag cattatttct tccccggctt
    12481 tcctgtgatg gcgcacccga tggcggaatg ggttttggaa acttattacg ccggccgctt
    12541 caaccaaacc gaacgcggca gccgcagcgt gtatgtgttt gagcagcccg aatcgcgcat
    12601 tacgccgatt atagagcata tcgagcaaac ttatcccggc gtgcgttcgt acagcctgcc
    12661 cagcgtcggt tggacgcatt cagacggcac gcaggtcaaa ccgcacattg agttcggcat
    12721 caaagccgaa ggagaagccg tcaacctttt ggacgcggcg tgggcggaag tgttgcacag
    12781 cttggacgga ttgggagcaa agctgaaaaa tcgggtaaac tgaaaactgt ttgcccaaat
    12841 cggacgatat ttaaggaaaa ccatgcaata ccgaatcaga agagaaaacg aagcacccga
    12901 agcaaaaaat gcgggggaaa ccttatggga gcgcgacatt atgcgcgaag tgctgctgtc
    12961 cgcctatcgg gacaggcgca gggagcggat gtggaaaaac atctggcgcg ccgtcagcac
    13021 cctgattctg gttgccctga ttgcaggcat tttccgaaaa gacgaagccg cattgcagtt
    13081 ggcgggcaac acgccgcata ccgcagtcgt caacctgtac ggcaaaatcg gcaacggcgt
    13141 agaagaccag gtcaaaaaac tcaaagacgg gatggaggcc gcctacaaaa acccgcaggc
    13201 aaaagccatc gtcatccgcg ccaacagccc cggcggttcg cccgtcgtct ccaacaccgc
    13261 ttttgaagaa atacgccgtc tgaaggcgca gcaccccggc atccccgttt atctcgtggc
    13321 agaggatatg tgcgcgtccg gctgctacta catcgcggcg gcggcggaca aaatctatgc
    13381 cgacccgtcc agcatcgtcg gcagcatcgg cgttatcggc agcagtttcg acgcgaccgg
    13441 cctgatggaa aaaatcggcg taaaacgcag ggttaaaatt gcgggcagca acaaaggcat
    13501 gggcgatccg ttttcgcccg aaacgcccga acagtcgaaa atctgggaag aaatgctgac
    13561 cggcatacac ggcgaattta tcaaagccgt caaaaccgga cgcggcggcc gcctcaaatt
    13621 ccgacaatat cccgacgtgt tcagcggtcg cgtgtacaca ggcgcggacg cgctgaaagt
    13681 cgggctggtg gacggactcg gcaatattta cagcgtggcg cgagacgtgg tcaaagctcc
    13741 cgatgtggtg gattacactc cgaaggacga tttcggcaga atcttgggca gacgcttcgg
    13801 agcagaactc aaagcaagcg tttcggaagc tctgcaggcc gtccggtagc gttgtttaca
    13861 gtatttctaa gggaatacca gaataaaaat atacacaaaa acactcacct ccccagtatg
    13921 ctgccactaa gtcagccgcg ttactattta ccgtgcactt aaagccgcaa aaggcaggtt
    13981 gtccccggga ggttttgtgc atcctgatac cgatcggttg ctgttgctgt tgctgttgct
    14041 caaaggacgg aaggccgccg ataaacgaga ttacctgttt gtcgctattg acgattttta
    14101 tactctgcca ttttgccgga caaaaccgca gacagtgctg ccaagtttct gaccgaacat
    14161 ctggctgacc cctgcttgta cctggttgag tacgtttact ctgacaatga taggtaatat
    14221 aaagagccgt ccaacatgct ttcggtgcag cttgttatga taatgggatt ggttggaggc
    14281 ttgcccgatt tgcttgtccg cagaccaacg gtaaggcgga gcgggttatc cgtaccttga
    14341 tggagatgtg gcatgaggaa cagtcgtttg acagtccgga acatcagcaa aaggagttgt
    14401 gctgctttat taatttaggc gctgtcctag ataactaggg aaattcaaat taagttagaa
    14461 ttatccctat gagaaaagcc gtctaagccg gtataaacaa aataaactca ttgggctatt
    14521 tgtcgcagg
//
LOCUS       DAXDZG010000059        14271 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXDZG010000059 DAXDZG010000000
VERSION     DAXDZG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.059"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(57..1157)
                     /locus_tag="ACFB78_001551"
     CDS             complement(57..1157)
                     /locus_tag="ACFB78_001551"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC1269518.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLRSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1482..3056
                     /locus_tag="ACFB78_001552"
     CDS             1482..3056
                     /locus_tag="ACFB78_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC1269519.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            3070..3258
                     /locus_tag="ACFB78_001553"
     CDS             3070..3258
                     /locus_tag="ACFB78_001553"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269520.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB78_001554"
     CDS             complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB78_001554"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC1269521.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB78_001555"
     CDS             4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB78_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC1269522.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(5699..6649)
                     /locus_tag="ACFB78_001556"
     CDS             complement(5699..6649)
                     /locus_tag="ACFB78_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC1269523.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB78_001557"
     CDS             7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB78_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC1269524.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB78_001558"
     CDS             8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB78_001558"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC1269525.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            9447..9932
                     /locus_tag="ACFB78_001559"
     CDS             9447..9932
                     /locus_tag="ACFB78_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC1269526.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB78_001560"
     CDS             complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB78_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC1269527.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            11904..13258
                     /locus_tag="ACFB78_001561"
                     /pseudo
     CDS             11904..13258
                     /locus_tag="ACFB78_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            13262..14223
                     /locus_tag="ACFB78_001562"
                     /pseudo
     CDS             13262..14223
                     /locus_tag="ACFB78_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
BASE COUNT         2988 a         3596 c         4051 g         3636 t
ORIGIN      
        1 aaaggggtgg aaatgccgtc tgaacggcgg aaatggcttt cagacggtat ttgcgctcaa
       61 taataatatc ccgcgcccag aatacacggt ttggatgcgc cggtcgcttt gtgcggacta
      121 ccgggaatgc ggttaatcca acacgccgcc aaccacgcaa atgcggcggc ttccacccat
      181 tgcggatcga ggttcagttc ggcggtgctg cgtaaggaaa cgcgtgtgcc gaaacattct
      241 gccaaatccg ccattaaaac aggattgcgg atgccgccgc cgcaaatgta catttgacgg
      301 gcatctgccg ctgcgtgtga gacggcgtcg caaacggttt gcgcggtgaa tcgggaaagc
      361 gtccgcaata cgtcgtatcg gttttcgccg ccgtcaaggt aggtttcgag ccaatttagg
      421 gcaaacagtt cgcgccccgt gctttttggg tggggttgtg agaaatacgg gtgggcgagc
      481 agcctgccga gcagttgcgg caatatgttg ccttgtgccg cctttgcacc gtttttgtcg
      541 taaggcagct gccagtgtgc ctgcgtccac gcgtccatca gcatattgcc cggccctgtg
      601 tcgaagccga aggcgggtgc gccggggggg agtacgctga tgttggcaat cccgccgatg
      661 ttcagtacca cgcgtgtttc cctgtcatcg cggaacaggg cttcgtgaaa ggcggggacg
      721 agcggcgcac cttgtccgcc ggcggcaagg tcgcggctgc ggaagtcgcc gacggtaaaa
      781 atccgcgtca gttccgccag cagcggcaaa tcggcaagct gtatgctgta accgtgttcc
      841 ggcgcgtgtc ggacggtttg cccgtggcag ccgagggcgg taatgtcgca cggagcgagg
      901 ttttgactgc acagcagttc ggcggcggtt tgcgcgtaca ggcggctgag ttcttgcgac
      961 aacatcctgc tgcggtgcag ttcgtctgtg cctgtgtcct gcaaatccag caatttgcgg
     1021 cgcaaccggt cagggtaggg ggtaaaggcg tgcccttccg cgcccagcca tttgccgccg
     1081 tccatccgta ccagcacggc atccgccccg tccatactgg ttcccgacat aatgccgatg
     1141 taaagctgtg tttccatcat cactcccaaa ctggtgcaaa acgccatttt aacgtgtatt
     1201 gacgctcgta taccgatttg ccgccgcagt gtaaataaag tgtaaataaa tgttttaaga
     1261 cgggtggaaa aatattataa tgcgcccgca acatccagta gtagaagtgt catacaaacc
     1321 gtttccggca gcagttttgc attcggtcag gtttgggggt attcggatgc ggttcagaag
     1381 gaaggatgcg tctgccatat cccgaaacgg cagttcgacc ggaggcagca atacagtgtc
     1441 ggcaacactc atgatttcca ccacattaaa ggaagattgc catggctcaa atccaaatga
     1501 gcgcaaatgt taaaaccatc aacgccgtct ttgccgccat gctggtaggt acagtcggct
     1561 attttattta ttggggcttg ggttataccc attacaatta cgccgcctta ttcattattg
     1621 ccacgatgtt cggtgtgttt atggcgttca acatcggcgg caacgatgtt gccaattctt
     1681 tcggcaccag cgtcggtgca ggcacgctga ccatcccgca ggctttgctg attgcggcgg
     1741 tatttgaggt cagcggcgcg gtcatcgcgg gcggcgaggt aaccgatacc atacgcaaag
     1801 gcatcgtcga tttgaagggt gttgatttcg aacccataca gtttgtgttt attatgatgt
     1861 ccgcgctttt ggcggcggcg ttgtggctgc tgttcgcgtc ccgcaaaggc ctgccggttt
     1921 cgacgacgca cgcgattatc ggcggcatcg tcggcagtgc gttgtgtatg gcgtttacgc
     1981 ataatgcgga cggggtggcg ttgatacggt ggggcaagct gggcgaaatc gggatgtctt
     2041 gggtattgtc gcccgtgttg ggcggcgcgg tgtcctattt cctgttttcg cgcgtcaaga
     2101 aaaacgtctt agattacaac gcttgggcgg aaggaacgct caagggcatc aagcaggaaa
     2161 aaaaggccta taaagaacgg caccgcctgt ttttcgaggg tttgtccgaa gccgaaaaag
     2221 tcgagtacgc caccaaaatg gcgcacgacg cgcaaattta cgacgaaccc gaattcgatc
     2281 cgcaagagct gcaatcggag tattaccgcg gtctttatgc gttcgacaac cgtaaaaaca
     2341 atgtcgattc ctacaaggcg ctgcattctt ggattccctt tatcgcttcg ttcggcacga
     2401 tgatgatttc cgctatgctg attttcaagg gcttgaaaaa cctgcatttg gggatgagca
     2461 acgtcaacag cttcctgacc atctttatga taggcgcggc ggtgtggatg gggacgtttg
     2521 tgtttgccaa aagcctcaag cgtaaagact tgggcaaatc gacctttcag atgttttcat
     2581 ggatgcaggt ctttaccgcc tgcggcttcg cattcagcca cggtgcgaac gatatcgcca
     2641 acgccatcgg cccgtttgcc gcgattatgg atgttttgcg gacgaacagc gttgccgcgc
     2701 aaagcgccgt ccctccgatt gcgatgctga ctttcggcat cgcgctgatt gtcggtttgt
     2761 ggtttgtcgg taaagaggtg attaaaaccg tcggtacgag tttggcggaa atgcatcctg
     2821 cttcgggttt taccgccgaa ctgtccgccg catccgtcgt gatgggcgcg tcgctgatgg
     2881 ggctgcccgt gtccagtacg catatcttgg tcggcgcggt actcggtatc ggtctggtca
     2941 accgcaatgc caactggaaa ctgatgaaac ccatcggttt ggcgtgggtc attaccctgc
     3001 ctgccgccgc tgtattgtcg atggccagct atttgatttt gcaggcagtg ttctgattgt
     3061 aaaatactga tgccgtctga acccgtgttc agacggcatt ttgttgatgg aatgtgcggg
     3121 cttgtgcctt atgcacaatc tgttctgtcg ggatatgctg tttggtatct tggtttcgga
     3181 acggtcggac acaaaggtta tgatatgccg ccgcctgttc ttgaaaacac ttatcctcgg
     3241 cagcaaaatg ccgtctgaaa aagcctttca gacggctttt gtacgttagc cgcaatcaca
     3301 ccgtttgcga atatttcgct ttggtttctt tgtggcgcag gtggtagtcg aagaccatgg
     3361 cgatgttgcg gatgaggaag cgtcctttcg gtgtaacggt cagcccgtgg ctgttcaggc
     3421 gcaccaatcc caaaccggcg agtttttcca aatccgccag ttcgtctttg aagtagcggt
     3481 cgaacgggat gccgaacata ctttcgtaaa tcctgtagtc gagcgcgaag cggcacatta
     3541 aatcctgaat gatgttgcgg cgcaggatgt cgtcctgatt gagctggtag ccgcgcatga
     3601 tgggcagtct gccttcgtcg atggcggcat agtaggcatc gatgtcgcgt tcgttttggg
     3661 aataggtgct gccgattttg ccgatggacg acacgccgat ggcgaccaaa tcgcaatccg
     3721 cgtaggtcga atagccttgg aagttgcgtt ggaggaagcc ttctttgagg gcgatggaga
     3781 gttcgtcgtc aggtttggcg aaatggtcca tgccgatgaa gacgtagccg cgctctgtca
     3841 gggtttggac gcagtattgc agcatatcga gcttctcttc gctgtcgggg acggcggcgg
     3901 tatcgatgcg gcgttgcggt ttgaacacgt gcggcaggtg ggcgtagtgg taaagggcga
     3961 ggcggtcggg atcgagcgac aaaacggtat ctatggtggt tttgatgctt tcggaagtct
     4021 gatgcggcaa gccgtaaatc aaatcgacgc tgacggattt gaaccctgcc tcacgggcgg
     4081 catcgatgac ttctttggtt tcttcgtagc tttggatgcg gttgactgcc gcctgcactt
     4141 tggggtcgaa atcctgaatg ccgacgctca tgcggttgaa gccgagtctg ccgagcatga
     4201 ggacggtgtc gcggctgact ttgcgcgggt cgatttcgat ggaatattcg ccggacggta
     4261 tcagttcgaa atgtttgcgg atcatgcgga agacacgttc gatctgttcg tcgctcaaaa
     4321 aggtcggcgt gccgccgccg aagtgcagtt gggcaagctg gtgccgtccg ttcagatgtg
     4381 gagcgagcag ttccatttct ttttcaagat attcgatgta ggtatcggcg cggcttttgt
     4441 ctttggtgat gattttgttg cagccgcagt agtagcagat ggtgttgcaa aacggaatgt
     4501 gaatgtaaag ggaaagcggt ttgtttaacg cgcccatacc gcgcaaatgt aaaactttga
     4561 tatattcgcc ttcgcggaaa ccgtcatgga aacggtcggc ggtagggtag gaagtatagc
     4621 gcgggccgct ggcgggcagg ctggcaatca gcgcgcggtc aaactcgggg cggtcatcgt
     4681 ttacattgtg attgttctgt atctgaatga ttttcatggt gtgtgtgcgg ttttatgatg
     4741 ttagtcaaat tttggatagt ttggtagaat gccacagtat gataaacctg tcttgatatg
     4801 tgtcaacaag cacataatcg ggatggggca aaacacacga aaaggtacgg tatggcttcg
     4861 cataatacta cacatcagat gaaaacgctg tgttcttcct gttctttgcg ggaactctgc
     4921 ctgcctgtcg ggctgctgcc caacgagttc agccaactcg atgccgtcat ccgccaaagc
     4981 cgccgcctga aaaaaggcga atacctgttc cgtgccggcg gagcctttac ctcgctcttt
     5041 gccatccgtt cgggcttctt caaaacaacc gtcgccagtc aggacggccg cgatcaggta
     5101 acgggtttct ttatgtcggg cgaactcatc ggcatggacg gcatctgttc ctatgtgcac
     5161 agttgcgacg cggtcgcctt ggaaaacagc gaggtatgcg agttgccgtt tacccatatc
     5221 gaagagctcg gtcagaatat ccccagcctg cgtacacact ttttccgcat gataagccgc
     5281 gaaattgtgc gcgatcaagg tgttatgctg ttgttgggca atatgcgcgc cgaagaacgg
     5341 attgccgcct tcctgttgaa cctttcccaa cgcctttatt cccgaggttt cgccgccaac
     5401 gacttcatct taagaatgtc ccgcgaagaa atcggcagtt atctcgggct gaaacttgaa
     5461 accgtcagcc gcacattatc caagttccat caggaaggat tgatttccgt cgaacacaaa
     5521 cacatcaaga ttctcaatct gcaggtgctg aaaaaaatgg tatccggttg ttcgcacgcc
     5581 atttgattaa cctgtacgaa catttcagac ggcattctca ataaacacag ggcagacgaa
     5641 aacatctgcc ctgtttgttg tatctgccgc aaagtgccgc ctgaaaaccg gcagccgcct
     5701 aaatcgaaaa atcctcgctg atgggcgtgt acagaatcct atccaccttc tcgcgtgtca
     5761 ggtgcggcgc aaacgcttgg ataaagtcgt aggcatatcc gcgcaaataa gtatcgctgc
     5821 gcaaagctat ccacgtcggc gacggctcga acaggtgtgc cgcatccaca agctgcaaat
     5881 cgccgtccgt atccgggttg tatgccattt tcgccatcag tcccacgccc aaacccaagc
     5941 gcacataagt cttcaataca tccgtatccg ccgccgccaa tgcgacatcc ggccgttcca
     6001 aacgggcttt ggcgaatgcc cgcgcgatgc tgctgcccgc attgaatgca aattcataag
     6061 taatcagcgg aaacctcgcc aaatcttcaa tacggagggg attcctgcat tcgagcagcg
     6121 gatggtcgtt cggcacaatc accgcgtgtg tccagtcgta gcaggaaagc cttcccaact
     6181 cggggtggtc gtctatccgt tccgtaacaa tcgccaaatc agattctccc gaagtaacca
     6241 tttgggcgat ggcggcaggg cttccctgtt tgatggtcag attgactttc ggatagcgtt
     6301 tcacaaaatc ggcaacaatc aacggtaggg catagcgcgc ctgcgtatgc gtcgtggcaa
     6361 ccgtcagcga accgctgtcc tgtccggtaa actcgctgcc gatattttta atgttctgta
     6421 catcgcgcaa aatacgttcc gcaatatcca aaaccacctt gcccggctgc gagaccgaaa
     6481 ccacgcgctt gccgctgcgg ataaaaatct gaatgccgat ttcttcttcc agcaatttga
     6541 tttgtttgga gatgccgggt tgtgatgtga ataaggcttc ggccgcttcg gaaacgttca
     6601 ggttgtgccg gtaaacttct aaggcgtatt tcaattgttg taatttcatg gcgggtcggt
     6661 gtgggtctgt gtcgggtggc tgaacattgt ttataattta tcatattttc ttgccggtac
     6721 ggtatggggc tttgccgttg tgtttgttgt ttttgtgcaa cggcaatcgt gcgatatgga
     6781 aaaaatcccc ctaaagtaat gacacggaat tgatttttcg gcatgataga ctatcaggaa
     6841 acaggctgtt ttacggttgt tttcaggcgt tgagtattga cagtccgccc cctgtttctt
     6901 tatagtggag actgaaatat ccgatttgcc gccatgtttc tacagcggcc tgtatgttgg
     6961 caattcagca gttgcttctg tatctgctgt acaaatctaa tgagggaata aaatgaccaa
     7021 acagctgaaa ttaagcgcat tattcgttgc attgctcgct tccggcactg ctgttgcggg
     7081 cgaggcgtcc gttcagggtt acaccgtaag cggccagtcg aacgaaatcg tacgcaacaa
     7141 ctatggagaa tgctggaaaa acgcctactt tgataaagca agccaaggtc gcgtagaatg
     7201 cggcgatgcg gttgccgtcc ccgagcccga acccgcgcct gtcgccgttg tggagcaggc
     7261 tcctcaatat gttgatgaaa ccatttccct gtctgccaaa accctgttcg gtttcgataa
     7321 ggattcattg cgcgccgaag ctcaagacaa cctgaaagta ttggcgcaac gcctgagtcg
     7381 aaccaatgtc caatctgtcc gcgtcgaagg ccataccgac tttatgggtt ctgaaaaata
     7441 caatcaggct ctgtccgaac gccgcgcata cgtagtggca aacaacctgg tcagcaacgg
     7501 cgtacctgct tctagaattt ctgctgtcgg cttgggcgaa tctcaagcgc aaatgactca
     7561 agtttgtcaa gccgaagttg ccaaactggg tgcgaaagcc tctaaagcca aaaaacgtga
     7621 ggctctgatt gcatgtatcg aacctgaccg ccgcgtagat gtgaaaatcc gcagcatcgt
     7681 aacccgtcag gttgtgccgg cacgcaatca tcaccaacac taaggctagg taatatcttg
     7741 ccgatgcatg aggttagcgg attttgtacc gggtactgtt gcaatattcg tgaaacgtcg
     7801 gccggtatcg atgatgtgaa acaaaccccg cttttgcggg gtttgttttt ttgggtggtt
     7861 ttctgaaacg gctatcgtca gaatcggggt gcaggttcgg attcggattc agattcatgt
     7921 ttgtgtccca ttgccgcgct ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     7981 cgcagacagt acaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc
     8041 actatatcgg ttgaaactct gattttaagg cggtaggatg tgggtttgcc catagcaagg
     8101 gaatcctttc tgtatcaagc cccgaaaggg ataattcata caaattcacg cctttccccc
     8161 tcattgggaa atggatggaa tcgtgcccga tgtgtgcggc actgtatgcc ggatatggtt
     8221 ttatcatcat cccttttcgg ttgaaacccc gccagttgca gcgattgagc ctaatcggtg
     8281 gcggaagttg ccgctttgca ttcggggcgg cgtgcagtgc ggtgctttga tatgccgttt
     8341 gtgtgttgaa acagggtggt cggtgcatac gggtacggta tggctaaagc taaaagtgaa
     8401 atacgctgaa acactgaatg agccgcttta ttgtttgtac gacctttgct gctttgctat
     8461 gatttaaatt ggattcgcac gccggatatt ttgggatatg aaagaattcg acttcatcaa
     8521 acggtatttg caaacaggta cggataagga tgtcgtattg ggcataggcg acgatgcggc
     8581 gattgtccgc ccgcgcgaag gcttcgattt gtgtttcagt gcggatatgc ttttgaagga
     8641 caggcatttt tttgcagatg tcaaacctga aggcttggct tggaaggttt tggccgtcaa
     8701 tatttcagat atggcggcga tgggtgcgat accgcgttgg gtgctgctga gcgcggcgtt
     8761 gcccgaattg gatgaagtat ggctggaacg gttttgcggc agctttttcg gtttggcaaa
     8821 aaagtttggc gtaacgttaa tcggcggcga tacgaccaag ggcgatatgg cgttcaatgt
     8881 aaccattatc ggcgaattgc cgaagggtag ggcgttgcgg cgtgatgcgg cggttgcggg
     8941 cgacgatatt tgggtgtcgg ggcgtgtcgg tatggcggcg gcggctttga actgccgtct
     9001 gaaacggtgt gtgttgccgg atgatgtgtt cgccgaatgc gaacaaaagc tgctccgtcc
     9061 cgagccaagg gttgggctgg ggcttgcgct gttgccgttt gcccgggcgg cacaggatgt
     9121 ttcagacggc cttgcgcaag atttggggca tatcctgacc gcttccggcg tgggggcgga
     9181 aatttgggcc gattcgctgc catctttatc tgtattgaaa gatattttgc cccgagcgca
     9241 atggctgtct tatactttgg cgggcggcga cgattacgag ctggttttta ccgctccgga
     9301 aagttgccgc agccgcgtgc ttgatgcggc ggaacggtgc ggcgtgccgg taacgtgcat
     9361 cggcaaaatc aacggaggat gccgtctgaa ggttttagat gccggcggca gggaattgga
     9421 actacattct ttaggattcg atcattttgg ctgattttaa acctgacttt gcgtggctgt
     9481 tgaaacggcc gttgtgtttt ttggctttcg gtttcggcag cgggctggct ccgtttgcgc
     9541 cgggcgcatt cggcactttg gcggcactgc ctttggcgtt tgtgctgatt ttgctcggca
     9601 tagacgggct gctgctggct tttgtgtgta tcgtgctgtt tatgtggggc atacgtattt
     9661 gtgcgtgtgc ggaacgtgaa acgggtgtca gcgaccacgg tgggattgtt tgggacgaga
     9721 ttgtcgccat gctgtttgtg ctggcgtttg tgccgttcag gtggacgtgg tggctggcgg
     9781 catttgttct gttccgtctg tttgatgcgc tcaaaccgcc ccccgtcggt tggtttgaca
     9841 agaatctgca cggcggtttg ggcattatgg cggacgatat ggcggctgcg gtgatgacct
     9901 tgattgtctt gaggattgca atgctgtttt aaacggggct gccttgtaaa aatgccgtct
     9961 gaaagccttt cagacggcat tgtttcggag gttaacgcgt taccggtttg tatttgatgc
    10021 gtttcggttt cgcgccttct tcgccgagtc ggcgtttctt gtcggcttcg tattcttgat
    10081 agttgccgtc gaagaacacc catttggagt cgccttcaca cgccaagata tgcgtggcta
    10141 tgcggtcgag aaaccagcgg tcgtgcgaaa tcaccatcac gctgccggca aattccaaca
    10201 atgcgtcttc caacgcgcgc agggtttcca cgtcgagatc gttggacggt tcgtccagca
    10261 gcaacacatt gccgccgccc aacaaggttt ttgccaagtg cagacggccg cgttcgccgc
    10321 cggaaagctg ccttgcgatt ttgctttggt cgctgccttt aaagttgaag cgtcccaaat
    10381 attggcgggc ggggatttca aactgtccga cctgcaaaat atcgcgacct tcggcaatgt
    10441 tgtcgaacac ggttttgtcg ttttgcaaac cttcgcggct ttggtcaatc aagctcattt
    10501 tcacggtttg cccgattttc acttcgcccg aatcgggctg ctctttgccc gcaatcattt
    10561 tgaacagcgt cgatttaccc gcgccgttcg ggccgatgat gccgacaatc gcgcccgccg
    10621 gcactttgaa gctcaaatcg tcaatcagca ctttatcgcc gaacgatttg gaaacattca
    10681 caaattcaat cacttcgtta cccaaacgct cggcaacagg gataaagatt tcctgagttt
    10741 cgttgcgttt ttggtattcg tagttgctca tttcttcaaa acgcgccaaa cgcgctttgg
    10801 gcttggcttg gcggcctttg gcattttggc gcacccattc caattcctgc ttcatcgcct
    10861 tcacgcgcgc ggcttcggat ttcgcctcgt tttccaagcg tttttctttc tgctccagcc
    10921 aagacgagta attgcctttc cacggaatgc cgtgtccgcg gtcgagttcc aaaatccatt
    10981 cggcggcgtt gtcgaggaag tagcggtcgt gcgttaccgc gaccactgtg ccggggaagc
    11041 gcacgaggaa ttgctccagc cattccaccg attccgcatc caagtggttg gtcggctcgt
    11101 ccaagagcag catatcgggc ttgctcaaca aaagtttgca taaagcgacg cggcgttttt
    11161 caccgcccga aagattgccg attttggcat cccaatcagg caggcgcagc gcgtcggcag
    11221 cgatttccaa ttcgtgttcc gcaccgccgc cagttgacga acccgccgcg ataatcgctt
    11281 ccaagcggcc ttgttcttcc gccagcgcgt caaaatccgc atcgggattg gcgtactcgg
    11341 catacacttc ttccaaacgt ttctgtgcgg cagccacttc gcccaaaccg ctttccactt
    11401 cctcgcgcac ggttttttcc ggatcaagct ccggctcttg cggcagatag ccgattttga
    11461 tgccgcccat cggcacggct tcgccctcaa attctttatc cacgcccgcc ataatccgca
    11521 gcacggtgga cttgcccgtg ccgttcaagc ccaagaggcc gattttcgcg ccggggaaga
    11581 aagaaaggga aatatcttta atgatggttt tctgcggcgg cacaaccttg ctcacgcgca
    11641 gcatagaata gacgtattgt tgggacatag ttttctcgat tcggtcaaac aaatttcaga
    11701 cggccatttt aaccgataat ttgatttaag ccagtttatc cgcgaaccgg tattgccaaa
    11761 atcgggcagg attcataaaa tccgcctatc cctttgaaat tatacagaca aaaaaataat
    11821 aatgataggg gatcgccgcg ccggcaacca tttcggattt tccaaagcaa atacttttca
    11881 aatcaaaaaa aaggatttac tttatgtcgg aatatacgcc tcaaacagca aaacaaggtt
    11941 tgcccgcgcc ggcaaaaagc acgatttgga tgttgagctt cggctatctc ggcgttcaga
    12001 cggcctttac cctgcaaagc tcgcagatga gccgcatttt tcaaacgcta ggcgcagacc
    12061 cgcacaattt gggctggttt ttcatcctgc cgccgctggc ggggatgctg gttcagccga
    12121 tagtggctac tactcagacc gcacttggaa gccgcgcttg ggcggccgcc gcctgccgta
    12181 tctgctttac ggcacgctga ttgcggtcat cgtgatgatt ttgatgccga actcgggcag
    12241 cttcggtttc ggctatgcgt cgctggcggc cttgtcgttc ggcgcgctga tgattgcgct
    12301 gttggacgtg tcgtcgaata tggcgatgca gccgtttaag atgatggtcg gcgatatggt
    12361 caacgaggag cagaaaagct acgcctacgg gattcaaagt ttcttagcga atacggacgc
    12421 ggttgtggca gcgattctgc cgtttgtgtt cgcgtatatc ggtttggcga acactgccga
    12481 gaaaggcgtt gtgccacaaa ccgtggtcgt agcattctat gtgggtgcgg cgttactgat
    12541 tattaccagt gcgttcacaa tctccaaagt caaagaatac gacccggaaa cctacgcccg
    12601 ttaccacggc atcgatgtcg ccgcgaatca ggaaaaagcc aactggttcg aactcttaaa
    12661 aaccgcgcct aaagtgtttt ggacggttac tccggtacag tttttctgct ggttcgcctt
    12721 ccggtatatg tggacttact cggcaggcgc gattgcagaa aacgtctggc acactaccga
    12781 tgcgtcttcc gtaggccatc aggaggcggg caaccggtac ggcgttttgg cggcggtgta
    12841 gtcggttgcg gcggtgattt gttcgtttat tctggcaaaa gtaccgaata aataccataa
    12901 ggcgggttat ttcggctgtt tggctttggg cgcgctcggt ttcttctcta tcttcttcat
    12961 ctacaatcaa tacgcactca tcctgtctta tatcttaatc ggcatcgctt gggcgggcat
    13021 tatcacttat ccgctgacga ttgtggccaa cgctttgtcg ggcaaacaca tggatactta
    13081 tttgggcctg tttaacggct ctgtctgtat gccgcaaatc gtcgcttcgc tgttgagttt
    13141 cgtgcttttc ccgatgctgg gcggccatca ggcaaccatg ttcttggttg caggcgcagt
    13201 cttgctgctg ggagccttct cagtctgtct gattaaagag atccacggcg gggtttgagc
    13261 gatgagcgat acccccgcta cccgcgattt cggcctgatc gacgggcggg ccgtaaccgg
    13321 ctatgtgctg tccaaccggc gtggtacgtg cgtcttcgtg ctggacttgg gcgggattgt
    13381 gcaggaattt tccgttttgg cagacggcgt gcgcgaaaac cccgtggtgt cgttcgacga
    13441 tgcggcttcc tatgcggaca atccgtttca gattaacaag cagatagggc gcgtggccgg
    13501 acgcatccgc ggtgcggcgt tcgacatcaa cggtaggact taccgcgtgg aggccaacga
    13561 aggcaggaac gcgctgcacg gcggttcgca cgggctggcc gttacccgtt tcaacgcggt
    13621 ggcggcagac ggccgacggt tatcccaacg atttggatat ttcctaccgc ttggacgagg
    13681 acggccggct taccgttacc tatcgcgcca ccgcgctcgg cgacacggtg ttcgacccga
    13741 cgctgcacat ttactggcgg ctggacgcgg gcctgcacga tgcggttctg catattccgc
    13801 agggcggaca tattccggcc gatgccgaaa aactgcccgt cttaacggtt tcagacggcc
    13861 tcgaagtatt tgatttcagc cggcccaagc cgccggatgc cgccgttgcc gccctgcgcc
    13921 gcgaaacggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg ccgtcccgcc
    13981 gcagtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg caacggcttg
    14041 gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt ttacgacgcg
    14101 ctggcgaccg aggcgcagac gctgcccgac agcctgcatt ggcccgaatt cggcaatatc
    14161 cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga atccctttct
    14221 taggagcttc ctaacaccgg tttcagacga cctttttata gtggattaac a
//
LOCUS       DAXDZG010000060        13807 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXDZG010000060 DAXDZG010000000
VERSION     DAXDZG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.060"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..2248)
                     /locus_tag="ACFB78_001563"
     CDS             complement(<1..2248)
                     /locus_tag="ACFB78_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC1269528.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARTILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVA"
     gene            complement(2245..3423)
                     /gene="hflX"
                     /locus_tag="ACFB78_001564"
     CDS             complement(2245..3423)
                     /gene="hflX"
                     /locus_tag="ACFB78_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC1269529.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(3429..4709)
                     /locus_tag="ACFB78_001565"
     CDS             complement(3429..4709)
                     /locus_tag="ACFB78_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC1269530.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRHAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(4861..5586)
                     /locus_tag="ACFB78_001566"
     CDS             complement(4861..5586)
                     /locus_tag="ACFB78_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC1269531.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(5659..6336)
                     /gene="radC"
                     /locus_tag="ACFB78_001567"
     CDS             complement(5659..6336)
                     /gene="radC"
                     /locus_tag="ACFB78_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC1269532.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            6363..6485
                     /locus_tag="ACFB78_001568"
     CDS             6363..6485
                     /locus_tag="ACFB78_001568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269533.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(6459..7808)
                     /gene="gshA"
                     /locus_tag="ACFB78_001569"
     CDS             complement(6459..7808)
                     /gene="gshA"
                     /locus_tag="ACFB78_001569"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC1269534.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            8060..9469
                     /gene="leuC"
                     /locus_tag="ACFB78_001570"
     CDS             8060..9469
                     /gene="leuC"
                     /locus_tag="ACFB78_001570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC1269535.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            9564..9818
                     /locus_tag="ACFB78_001571"
     CDS             9564..9818
                     /locus_tag="ACFB78_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269536.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            9880..10521
                     /gene="leuD"
                     /locus_tag="ACFB78_001572"
     CDS             9880..10521
                     /gene="leuD"
                     /locus_tag="ACFB78_001572"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC1269537.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            10688..11959
                     /locus_tag="ACFB78_001573"
     CDS             10688..11959
                     /locus_tag="ACFB78_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1269538.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            11975..12706
                     /locus_tag="ACFB78_001574"
     CDS             11975..12706
                     /locus_tag="ACFB78_001574"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC1269539.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            12740..>13807
                     /gene="leuB"
                     /locus_tag="ACFB78_001575"
     CDS             12740..>13807
                     /gene="leuB"
                     /locus_tag="ACFB78_001575"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC1269540.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENSVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
BASE COUNT         3271 a         3697 c         3628 g         3211 t
ORIGIN      
        1 gggcgacggt acgcaaatct tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat
       61 ccgtcccgcc cgctgcggcg gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga
      121 tgccgtcgcc tatcatcagc acttttttcc cttctttttg caaggcttcg acgtattcca
      181 gcttgtcttc cggcattgct tgggcgcggt agtgcgcgat acccaaggca cgggcggttt
      241 cagcaactgc ttcttcacgg tcgccactga ggatgtgcag cgtcaggttt ttgcctgcca
      301 actgccgcac tgcctccgcc gcgctgtctt tgagcgggtc ttgcaggtag aacacggctt
      361 ggaaaccgct ttgactgccg aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt
      421 tacctgaaat ttcggcaaca taggatgccc tccccaatgc ccaaacctgt gtttcgccgt
      481 tgacggtcag ttgcgcaccc acgccttccc cgatgcggtt gaggcgttgt ccgacttgga
      541 tttcggggac gctgccgcct gaaatgcggc agttgagaat ggtgcgcgcg agcgggtgtt
      601 cggactgctg ttccaaactt tgcgccactg ccagcacttg ggcttcggtc atgctgccga
      661 gcagttcaat ccggcggaca gcgggattgc cttgggtcag cgtgccggtt ttgtcgaaaa
      721 tgatgtcggt ggtttgggag agggtttcga ttgcctgctt tccgccgatt aaaatacctt
      781 cgcgcgccag cgtaccggta gaagctgcca gcgcggtcgg cgtggcaagc gacagggcgc
      841 aggggcaggt aatgaccagc agggcgacgg taatccacaa tgcggtgtgc gcgtcggcgt
      901 acagcgtcca accgatgaag accgggacgg caagcaggag ctcgccgaat atgaaagatg
      961 aggcgtattg ttccgccaac tcggcagtgc gcggtttttg cgccaaggcg cggtcgagca
     1021 ggcggacgat gtgcgacagt cgcgtgccgc cgccggtgcg gtcggtgcgt ataatcagag
     1081 ggctttgcgt gttgagtgta cctgcggtta ctttttcaga cggcattttg gcgacgggca
     1141 ggctctcgcc ggtcagcata gattcgttga cggcactgtt tccttccagc accgtgccgt
     1201 caacggggat ggtttcgccg ggtttgacca tcacaatatc gcctgcttgg agtttgacga
     1261 cggcggattc gcggacatcc tgtacggcag gatagccggg catccggtgg cagaaggcgg
     1321 gtatcagttt caccagcctt tcggcggcat cgcctgcctt gcgtcgggca atgtgttcca
     1381 taaagcgtcc gcccagcagg aaaaacagca gcatcgcgat ggattcgaaa tacatcccct
     1441 gccctgcgtt ggtggcgagg ctgtaaatgc cggcgatgaa ggtcatgatg atggcggcgg
     1501 caatcggcgt gtccataccg gcgcggcggt ttttcaggtc gcgcaacgcg ccttgataaa
     1561 acgggacggc gcaataaaat acgacgggca gcaccatcag gaagccgccc caatggagga
     1621 tctgcaggaa atcgggttcg atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca
     1681 tcgtctgcat catacccagt ccggcaacgg caaggcggac gatgtattgt ttgcgttctt
     1741 tttggttggc ggcttcgatt ttttgcgcgt catagggtgc ggcggtgtag cctgtctgcc
     1801 ggattttcaa cagaatgtcg gagaggcgga ttttgccgtc gtcccagacg acgcggcagc
     1861 ggtgcgtgct gtaattgagg tcgatgcgga cgatgccgtc tgtgcgcaaa agctgctgtt
     1921 cgatcagcca gacgcaggcg gcgcaggtaa tgccgctgag catcagcact gcttcgtgcg
     1981 tgccattatg ggtttccaca aagtcggatt ggacttcggg caggtcgtac aggcggattt
     2041 ggtcgaggat ttcttggggc ggcagttcgg tttttttcgc gtcggcggtg cgtcgtttgt
     2101 aataactgcc caagccggaa tcgatgatgc tttgtgcgac tgcctgacag ccgacgcagc
     2161 aggtttcgcg gtcttcgcct tcgtagcgga cggtcagatg caggttttcg ggaacgtcca
     2221 gcccgcagtg gaaacaggtt tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc
     2281 ggcacaatac tcggcaatgg cttcgcgcag tgcgtctata ccggtatttt cagcaacgga
     2341 aatgcggacg gcggcaattt ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc
     2401 agacggcagc aggtcggttt tgttgtacac cttgatgcac ggaatatcgt gggcatggat
     2461 ttcttgcagt acgttttcca cgtcttcaat ctgctgtccg ctgttcgggg cagcggcatc
     2521 gacgacgtgc agcagcacat cggcttgcgc ggtttcttcc agcgtggcgg aaaatgccga
     2581 aatcagtttg tgcggcagat cgctgacgaa tccgacggta tcggtcagga taatgctgca
     2641 tgcgggactg atgtacagcc gccgcgccgt cgtgtcgaga gtggcgaaaa gctggtcttt
     2701 cgcatatatg cccgacttgg tcagccggtt gaacaggctg gatttgccga cattggtata
     2761 gccgaccagc gcaaacgttt tgattctgcc cgactcgcgg gacttgcggc gcagggcgcg
     2821 ctgttttttg aggttggcaa gctgtttttt caaggcgttg atccgatggg cggttaatcg
     2881 gcggtcggtt tccagtttgg tttcgcccgg ccctttcatg ccgataccgc cgcgctggct
     2941 ttgcaaatgt ccgtaaccgc gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc
     3001 gacttgcagc ctgccttcct gcgtgcgggc gcggcgggcg aaaatcgcca gaatcagccc
     3061 cactctgtcc aatacgcggc attggaggat tttttccaaa ttgcgttcct gcgtgggagt
     3121 aagttcgtgg ttgaatacga ccaaatcaat gccgtctgcg gcaactgctt ccgacagctc
     3181 cgccgccttg cccgtgccga caaacagcgc agtgtgcggg cggtcgcgtt tggcagtctc
     3241 cacgcgtacg gaatcgccgc ccgccgcctt gaccagctcg acggcttccg ccaatgccgt
     3301 ctgaaaaccg ttcagacggg cggcattgct gcccgtatca tctttatcca acattacgcc
     3361 caccagcatg acgcgttccg gttgcgcctg agtggcactg ttccgtcctg ttctacctga
     3421 cataactgtc aatcctgtaa ataaagggtt acaccgcgtt ccgggctgtc ttccggagta
     3481 agcctgatgc ggcttcccgc cattccgctg acgctgaaga caatgccgcc cgcttcgtgc
     3541 cggagtatgg acaggctgtt gccgaactgc cgccggtagg tataacgggt acacgcttcc
     3601 agacaccagc gtccgcgcca gtggatgaaa tatttttcgc cttttctaac cgcaagtgtc
     3661 tcgccgccgt ttacctcaaa ataagggtaa tcgggttttc gggttctaaa ctgccatttc
     3721 gcctgcgcgt gccgtccgtt gcggacatag tcgaataccg ccgtataaag cgtgccgtat
     3781 tccaaaggtt tgagcgggaa aagggcgaat tggtgcgcgg tcagcctgcc gttagggtcg
     3841 ttgccggcgg ttaaaaccct gacggggcgg atttcgtttt taccctgata cagcttgaaa
     3901 cttttcatcg caattttgcc tgccgcctcg gaaaaatcaa tgctggcagg attgcctgtg
     3961 atttcatatt ccggcacggg gtcgggacgt tccccgtaaa aataaggcag cgcgccgccg
     4021 ccaaccggat aggcggtata aagcaattcc gttacgggca tggcttcgtc agcataaacg
     4081 gccgcaccgt tgtggcaagc gttgcggtaa tatttccgtc ctgcttccgg ctgccgcctt
     4141 ccttttgcac aggcgcgctc gaagctgccg ttgccctgat tgaatacgag gacggttttg
     4201 ccgttttcgc gcacaaatgc cgcacctgct tcgtcggtat ggcggtcaag cagcgaaagg
     4261 cggtgataga cagcactcat caatccgtct acttggcgtt gctgcgtgcg gatgtcgctg
     4321 tcggacgatt cggcggcttc ctcttccgtg ctgatgtttt catgcacgcc gttgtagaga
     4381 tacccggcaa ggcgtgtgcg ttcggtcagc ttttgtgcgg tgtagtgcgg attgtcggga
     4441 tggtgttccc cgtgtccgtc ttcgggattg agcgtgagat agcgtgcgtg cctgcgggcg
     4501 gaattttcca aaaccggcgc gtgtgccagc gcgtgcaaac cgatttgtgt gcggatgcgg
     4561 ttgaggtagt gcagcgcatc gccgccgtcc ctgatttgcg gtgcggacgg atagacaagt
     4621 tcgcccgcgg gcagggattg gttttgggta tggtagaaaa cgcctgccgc cgagcccaat
     4681 agaagcagcc aaataaaaag ggatttcata atgccgccct cccgacgcgg caacggggtg
     4741 ttttatcaaa acggcaaact tgccaatgcg gtttccgctt gccgattgcc aaaccggcaa
     4801 acctttcccg tgccggtgcc gaacccgatg ccgtctgaac cttcagacgg catcgggtgt
     4861 ttatttgtcc actcggacgg gtgcaggttc gtattgtgtc gattcgtcgc cgtaatacag
     4921 cacgccgagt ttgacgggga tgcgtccctg cgatttgcgg tgggcgttgg aatcgcgcaa
     4981 ggaataggcg cagccgcagt attcctgctg gtagaagttt tcgcgtttgc tgatttcaat
     5041 catacgcgcg ccgccgccgc ctttgcgcca gttgaagtcc caataggcca catcatcgta
     5101 aggcgcggcg gcacggtgtc cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat
     5161 gcccagcgaa ctggtaaaga cgggaaaacc gtgttcgtgc gcgtattgcg ccgctttttc
     5221 aaaacgcata tcgaaacaca tggtgcagcg gatgccgcgt tccggctcaa actccatgcc
     5281 tttggctttg gcaaaccatt ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa
     5341 agggatgccg aacttttccg caaagcgcat gttttcctct tttcggagca tatactcttt
     5401 gtgcggatgg atattgggat tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat
     5461 ggcttccatc acttcgccgc tgcacggggc gcagcaggaa tgcagcaaga cttttttatg
     5521 tccgccgggc gggacgagta tggggcggtc aatgtcggta acggtaggtt tgttttgtgt
     5581 ttccattaac ttaacaatat ctgtcgaata cggtcaaagc ggctatttta ccagagccgc
     5641 cgaatgtaaa acagggtgtc agggcatcag cccgagctgc ctgaacgaac ggacggtttg
     5701 cgaggtaacg ataaaatggt cgagcagcga aacatcaacc agcgacatgg cctgtgccag
     5761 ccgccttgtg aacatgatgt cttcctgcga aggttcgggc gagccgcccg gatggttgtg
     5821 cgcgataatc aggctgtcgg catattcgtc caatgccagt ttgacgattt cgcggatgta
     5881 aatcgtgttt tccgcaaccg taccgcgcga cagctctctg accgcaatca gttggttttg
     5941 gcggttcagc agcagcgcga cgctgacttc gactttttcc tgccccaaat gaaagcgtaa
     6001 ataatcggca acggtatcgg gatcggataa ggtaatttct tcctgcaatt cttcctccaa
     6061 tatccgccgc ccgatttccc tgaccacggc gaactgtgtg aaacttgccg tccccatccc
     6121 tttgtatgcc gacagtttgc cgacctccgc gctcatcagc ctccccaagc tgccgaactc
     6181 ctgcaatagg tagcgcgcca aatcgaccgc gctcatcccg cgcgtcccga cgcgtaaaag
     6241 gattgccaaa agttcggcat cgctcaaagc cgccgccccg cgttccaaca gcttttccct
     6301 gggcctttcg ccttccggcc attgcttgat gctcatgttt tccctttttt aaatagtctg
     6361 agatgccgtc tgaaacagat gttcccacac gccaaatatc cgaaatgccg tctgaaccgg
     6421 atttccgctt cagacggcat ctgtatttca aaacagtctt acgccgtttc ttccaactcg
     6481 attgaagccg ccaaaagcga cagacgtgcc attacgccgt aaacgtagag gcggttatgt
     6541 tcgttgtcca cgtcgcagtc tttttcagaa cgcggcatac cgagcgaatc ccattgttcg
     6601 aatacgcgtt tgcaggcttc tggagcttct tgcgaattat cgccgttacc ggtaggaatg
     6661 ctgttggaca gcggcacaaa caccataccg ccggcgttga ggttttcgtc cgcaccgcgc
     6721 ccttcgtgta cgcggaaaaa gccgccgatg acgaaacggt ccatcatata gacgacgggt
     6781 tcgcacaccg cgccgttcaa ggtttcatag gtataaaccc cttcttggac aatcacttcg
     6841 ctgacttcca agccctcttt caccttcgcc attttgttgc ggtttttgcg gttcaagccg
     6901 cgcacttcgt cggaggattt gacgctcatc acacccatgc cgtaagtgcc ggcatcggct
     6961 ttgacgatga cgaaaggttt gtcggcaatg cccgattcgt cgtatttggc ttgaattttc
     7021 gccagcacgc gttctaccgc ttccgccagc gcatcttcac cttcgcgccc ttggaagtcc
     7081 aaaccgccga ttttttcaaa atacgggttg atctgccatt catcgatgtc aatcagcttg
     7141 gcaaattcgg cggcaacttg gttgtacgcg ccgaaatgat ttgttttgcg gcgcgtcgtc
     7201 caaccgccgt gcagcggcgg caaaacggtt tggccgatgc ctttgagaat gtcgggaatg
     7261 ccggcggaca aatcgttgtt caacaaaacc acgcaaggcg aaaagccgtc ggcaaggtgg
     7321 acgcggtcgc gggttcgcag caaaggctca agcaggattt tgtcgcccaa tgcagtttca
     7381 aattcggccg gttcggttac ttccggattc aggctgccca agcgcacttc acaccctgcc
     7441 gaacggaaaa tctcgccgag ggcgtaaacg ttttgcaggt aaaacgtatt gcgcgtgtgg
     7501 ttttcgggaa taatcaatac ggatttctcc gtttcgcagg cgcgttgcac cgcatcttgc
     7561 gcggcaaccg ccgccagcgg gatgaaattc ggattcagat tattgaagcc gcccggaaac
     7621 aaattcatat cgatagacga aattttgtaa ccggcgttgc gtatatcgac cgaaccgtaa
     7681 aacggcgggc ggtgcgcgtt ccactgcgtg cggaaccacg cctcgatttt ggcgtgattg
     7741 gacaggattt ttgcctcaaa cgcctgaagt tgcgccgaat gttcgggcga cataaccggt
     7801 aatttcatct ttcgtctgcc tctgtgtgtt ttttgacaag gtacagataa taatgctttt
     7861 gaaagaatga cgacaagtat tctttcgtat cgaatcattc tttagatata acgaaatgaa
     7921 caatatgtct aattttttcg ttaaaattgt ttacaaaata acaccgactc aaaaattaga
     7981 caaaatctgt tgcgcagtat aaagaatacg tctaaaatcc gcccaatccg catcaccatt
     8041 tatccaaaag aacaacatca tgacggcaca aaccctctac gacaaactct ggaacagcca
     8101 cgtcgtccgc gaagaagaag acggcaccgt cctgctttac atcgaccgcc atctggttca
     8161 cgaagtaacc agcccgcagg cgtttgaagg tctgaaaatg gcggggcgca agctgtggcg
     8221 catcgacagc gtggtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg
     8281 catccaagac ccgatttcca agctgcaagt cgatacgttg gatcaaaaca tcaaagagtt
     8341 tggcgcactc gcctacttcc cgtttatgga caaaggccag ggcatcgtac acgttatggg
     8401 gcccgaacaa ggcgcgaccc tgcccgggat gaccgtcgtc tgcggcgatt cgcacacttc
     8461 cacccacggc gcattcggcg cactggcaca cggcatcggc acttccgaag tcgaacacac
     8521 gatggcgacc cagtgcatta ccgcgaaaaa atccaaatct atgctgattg ccgttgacgg
     8581 caaattaaaa gcgggcgtta ccgccaaaga cgtggcgctc tacatcatcg ggcaaatcgg
     8641 cacggcgggc ggcacgggtt acgccgtcga gttcggcggc gaagccatcc gcagcctttc
     8701 gatggaaggc aggatgaccc tgtgcaatat ggcgattgag gcaggcgcgc gttccggtat
     8761 ggttgccgtc gatcaaacca ccatcgacta tgtcaaaggc aagcccttcg cacccgaagg
     8821 cgaagcttgg gacaaagccg tcgagtactg gcgcacgctg gtgtctgacg aaggcgcggt
     8881 ttttgataaa gaataccgtt tcaacgccga agacatcgaa ccgcaagtta cttggggcac
     8941 gtcgcccgaa atggttttaa acatcggcgg caaagtgccg aatcccgccg aagaaaccga
     9001 tccggtcaaa cgcagcggca tagagcgcgc actcgaatat atgggtttga aggccggtac
     9061 gccgctgaac gaaatccctg tcgatatcgt ctttatcggc tcttgcacca acagccgcat
     9121 cgaagacttg cgcgaagccg ctgctatcgc caaaggccat aaaaaagccg gcaacgtaca
     9181 gcgcgtgtta atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt
     9241 ggacaaaatt ttcatcgaag ccggttttga atggcgcgaa ccgggctgtt cgatgtgcct
     9301 tgcgatgaat gccgaccgcc tcgcgccgag gcaacgctgc gcctccacgt ccaaccgcaa
     9361 ttttgaaggc cgccaaggca acggcgggcg cacccacctc gtcagccccg ctatggccgc
     9421 cgccgccgcc gttaccggac actttaccga catccgcacg atggcgtaag tattttcagg
     9481 cagtctttaa gaatttgacg aggccgtctg aaatatgtcc cgtaaggcag aaacacctaa
     9541 tattcaaacc aaggagttta accatgaaca aacttttcgt taccgccctg tccgccctcg
     9601 ccttgtccgc ctgcgccggc acttggcagg gcgcgaaaca agacaccgcc cgcaaccttg
     9661 acaaaacaca ggccgccgcc gaacgcgccg ccgaacaaac aggcaacgcc gtcgaaaaag
     9721 gttgggacaa aaccaaagaa gccgtcaaaa aaggcggcaa tgccgtcgga cgcggcattt
     9781 cccatctcgg caaaaaaatc gaaaacgcca ccgaataacc gatttgcgat gccgtctgaa
     9841 accgtttcag acggcatcgt cagcacaaga cgcaaaccca tgaaagcctt taccaaaatc
     9901 accgccatcg tcgccccgct cgaccgcagc aacgtcgata ccgatgccat catccccaaa
     9961 caatttctga aatccatcaa acgcagcggc ttcggcccca acgcctttga cgaatggcgt
    10021 tacctcgacc acggcgaacc gggcatggac aacggcaaac gcccgttgaa ccccgatttt
    10081 tccctgaacc agccgcgtta ccaaggcgcg caaatcctgt tgacgcgtaa aaacttcggt
    10141 tgcggctctt cacgcgaaca tgccccttgg gcattggacg actacggctt ccgcgccatt
    10201 atcgccccca gcttcgccga catcttcttt aacaactgct acaaaaacgg ccttttgccc
    10261 atcgtgttga ccgaagaaca agtcgaccgg cttttcaaag aagtcgaagc caacgaaggc
    10321 tatcggctct ccatcgacct tgccgagcaa accctgacca ccccgggcgg cgaaacattc
    10381 acattcgaca ttaccgaaca ccgcaaacac tgcctcttaa acggcttgga cgaaatcgga
    10441 ctgaccctgc aacacgccga caaaattaaa gcctttgaag aaaaacgccg ccaaagccag
    10501 ccttggctgt ttaacggtta aaacctatgc cgtctgaagg ctgttcagac ggcattcctg
    10561 ataacatata attgctgcgg ctaatgtaaa cagaggggcg ataaattgtt ttcatctttt
    10621 aagcgggctt agcaacactc taaattaatg caactctcca tttcaaataa atttttagat
    10681 ttttaatatg caacaaatta aatttattga cttattttcc ggaatgagtg gcatcaggaa
    10741 aggattcgaa caagcctgtc ggaaacagtc ggttgcttgc gaatgtgttt ttacttcaga
    10801 aatcaaaccg gcagctttgg aagtattgaa gcaaaactac cctgatgaag tgccgtatgg
    10861 agatataacg aaaattgaaa caggggatat tcccgatttt gacatcctgt tggcaggctt
    10921 cccttgtcag gctttttctt tcgccggaaa aagattaggc tttgaagata cacggggaac
    10981 gcttttcttt gatgtggcaa ggattttaaa ggcaaaaaaa ccaaaaggtt ttattttgga
    11041 aaatgtggaa ggattggtga cacacgatag aaaagattcg acacaaaaaa tagggcgcac
    11101 cctgaccgtt attttggaaa ccttggaagc attgggctac tatgtttctt ggaaagtttt
    11161 aaatgcaaaa gatttcggca ttccccaaaa caggaagcgc atttatctga caggcagttt
    11221 gaaatccaaa cccgacttat ctttcgaaac aagcccaagt ccgaaattaa aaaatatttt
    11281 ggaatcggga ctgcctactg aaagcagccc tttcatcaaa aaattgctaa aaaaatttcc
    11341 cccgtccgaa ctgtacggaa aatcagtaaa agacaaacgg ggagggaaaa acaatattca
    11401 cagttgggat attgaattaa aaggcgcagt aaccgaagaa gagaagcaat tgttaaatat
    11461 ccttctaaaa gaacggagga aaaaaaaatg ggcttcagaa atcggcatag attggatgga
    11521 tgggatgcct ttgacaaaag cgcaaatttc aactttctat aaacaccccg atctccaaaa
    11581 tattttggac agcctgacag acaaaggcta tttggtttta gagcatccga aacaaaaaat
    11641 tggcgggcaa agaatcaaag atgaatccct gcccaaaggc tacaatattg tttcaggtaa
    11701 aaaatctttt gaaatcaata aaatattaga tccaaacgat gttgcgccaa ccttggttgc
    11761 aatggatatg gagcaccttt tcgtcgttga caacggcggt ttgcgtacac ttaccggaaa
    11821 agaagggtta cgcttattcg gctatccgga cgattattcg tttgatattc ccaaaaaaga
    11881 cagatgcgat ttattgggta ataccgttgc cgtccctgtg attaaggcgg tatctgaaag
    11941 acttctgcat actttataaa ccgacaggga taaaatgata aaacttactg cacaacaaat
    12001 atttgataag ttgctggatg aagaaaaaat cttatcggcc aatggtcaaa ttagattttt
    12061 cttaggagat gtggatatta tcgtcaaaca aaaagatgtt gtcggcaaca tcattcagga
    12121 atggctcggc ggatggttga gaaaaaggga aattgaattt gatgtttcaa ccaataccca
    12181 aatgcctccc gatttctttt taaataaaaa agaccgcagc agagaattgt tggaagtaaa
    12241 ggcgttcaac cgaaatgcct gcccgggttt tgatattgca gattttaaaa tgtattctga
    12301 tgaaatcatt cataagccct atatgctgga tgtagattat ttaatattcg gttacgatat
    12361 ggacgacaac ggcaatgtaa ccatcaagga tttatggctt aaaaaagtat ggcaaattac
    12421 cagaagcatg gatgggtggg caattaatct tcaagtcaaa aaaggcgtgg tgcataaaat
    12481 ccgcccgggt gtttggtaca gcataaataa aaagaatatg cctatgtttg aatgcttgga
    12541 agattttgtt tccgcaattg aagaaaccgt ttatcaaaac ccggcaacac ggcataacgc
    12601 atccttatgg aaaaagaaat ttgaggaagc ttataaaaaa cactacaacc gatcgatttc
    12661 cataccccgt tggcacgaaa ttgcacacaa atacaaaaag aaataaccct tcaaccatca
    12721 acccataagg aaacttgaaa tgaccaaaca tatcgccatc ctccgaggcg acggcatcgg
    12781 tcccgaaatc gtcgccgaaa ccgtccgcgt actcgacaaa tttatcgccc aaggcttgga
    12841 tgccgactac gaatacgcgc ctttgggcgg cgaagcctac gacgaatacg gccatcctta
    12901 tccagaattt acgcaaaacc tctgccgcaa agccgatgcc gtcctgcttg gtgcagtcgg
    12961 ttcgccccaa tacgacaatc tcgaccgtcc gttgcgcccc gagcgcggct tgttagcaat
    13021 tcgtaaggat ttgaacctgt ttgccaattt gcgtcctgcc gttttatatc ccgaattagc
    13081 caacgcctcc acgctcaagc ctgaaatcgt tgccggtttg gatattttga ttgtgcgcga
    13141 acttaccggc gatatttatt tcggcgaacc gcgcggcatc cgtgttttgg aaaacggcga
    13201 acacgaaggc tacaacacca tgaaatacag cgaaagcgaa atccgccgca tcgcccacgt
    13261 ggccttccaa tccgcccaaa aacgcagcaa aaaagtctgc tccgtaggca aagccaacgt
    13321 tttggaaacc accgaactgt ggcgcgaaat ctttgaagaa atcggcaaac aatatcccga
    13381 tgtcgagctt tcccatatgt atgtggacaa tgccgccatg caactcgtgc gcgcgcccaa
    13441 acagtttgac gtgattgcca ccggcaacat cttcggcgac atcctctccg acgaagcctc
    13501 catgctgacc ggctccatcg gtatgctgcc ttccgcttct ttggacgaaa acggcaaagg
    13561 cctgtacgaa ccgtctcacg gctccgcccc cgacatcgcc ggacaaaaca aagccaaccc
    13621 gctggccacc atcctctcgc ttgccatgct gctgcgttac agcctgaacg acgaagcgcg
    13681 cgcgcaacaa gtcgaaaact ccgtccaaaa agtgctgcaa caaggcttgc gtaccggcga
    13741 tatttacgaa gaaggcacaa aactcgtttc ctgctccgaa atgggcgacg cggtactcgc
    13801 cgccttg
//
LOCUS       DAXDZG010000061        13550 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXDZG010000061 DAXDZG010000000
VERSION     DAXDZG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.061"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            129..619
                     /locus_tag="ACFB78_001576"
                     /pseudo
     CDS             129..619
                     /locus_tag="ACFB78_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            656..922
                     /locus_tag="ACFB78_001577"
                     /pseudo
     CDS             656..922
                     /locus_tag="ACFB78_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..2047
                     /locus_tag="ACFB78_001578"
     CDS             1424..2047
                     /locus_tag="ACFB78_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC1269541.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2159..3541
                     /locus_tag="ACFB78_001579"
     CDS             2159..3541
                     /locus_tag="ACFB78_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1269542.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3545..4069
                     /gene="ssb"
                     /locus_tag="ACFB78_001580"
     CDS             3545..4069
                     /gene="ssb"
                     /locus_tag="ACFB78_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC1269543.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4442..5365
                     /locus_tag="ACFB78_001581"
     CDS             4442..5365
                     /locus_tag="ACFB78_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC1269544.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFB78_001582"
     CDS             complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFB78_001582"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC1269545.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFB78_001583"
     CDS             complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFB78_001583"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC1269546.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            9475..10971
                     /gene="ccoG"
                     /locus_tag="ACFB78_001584"
     CDS             9475..10971
                     /gene="ccoG"
                     /locus_tag="ACFB78_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC1269547.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
     gene            10974..11522
                     /locus_tag="ACFB78_001585"
     CDS             10974..11522
                     /locus_tag="ACFB78_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC1269548.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(11864..13039)
                     /locus_tag="ACFB78_001586"
     CDS             complement(11864..13039)
                     /locus_tag="ACFB78_001586"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC1269549.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEITEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(13197..13313)
                     /locus_tag="ACFB78_001587"
                     /pseudo
     CDS             complement(13197..13313)
                     /locus_tag="ACFB78_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13476..13550)
                     /locus_tag="ACFB78_001588"
     tRNA            complement(13476..13550)
                     /locus_tag="ACFB78_001588"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13515..13517),aa:Glu,seq:ttc)
BASE COUNT         2940 a         3373 c         3899 g         3338 t
ORIGIN      
        1 gcaagcgtag gtcggatact tgtatccgac aaaaccttta acattcccat cattgcaatc
       61 cattgcagca atgtccaaaa tgtcgaattc aagaatccgg cctacaaaat cattccgagc
      121 ataatactat gaaataccgt cgtttttacc gcaatggcgg cacttacttt tttacggttg
      181 taaccaataa acggcagaag attttgaccg atgatgcggt gcgtttggct ttacggcagg
      241 cggtaatggc ggtgcgcgaa cggaatccgt ttgaaatttt ggcatgggtg ttgatgcccg
      301 atcatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg gaacgctggc
      361 ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt tggaaaaacg
      421 cttttgggaa catactatcc gcggtgaggc cgattttgcc tgccattttg attatctgca
      481 tttcaatccg gtcaaacatg gctatgtagg acaaatttcc gattggcggt tttctacgtt
      541 tcaccgttat gtcaaacagg gtatttatcc gcataattgg ggtggcggca atgcggactt
      601 ttctattgaa tacgattgaa gtaatgtcgg attcgagaat ccgacctacg gaaaactgaa
      661 agagcatcgg ctgctggacg gcattatgcg caaagcccgc cgcaaccgcc cgctgccgga
      721 ggcgcaaacc aaacgcaacc gatatttgtc gaagacccgt tatgtggtcg aacaaagctt
      781 cggtacgctg caccgtaaat tccgctatgc ccgggcggcc tatttcggac tgattaaagt
      841 gagtgcgcaa agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct
      901 aagtgcgccc gctgccgcct aaaaggcggc ccggatgcct gattatcggg tattcaaggg
      961 ggattaaggg ggtatttggg tagagttagg agtgattggt ggcggaaaca gccgaaaatc
     1021 tgtgtcaggg tttcagctgt cgggagggaa gggaattttg caaaggtctc aagatatagt
     1081 ggattaaatt taaaccggta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     1141 cactatattt ccgcctgctg ccgcccgaaa agcgtaatgc gctgatggtg ctgctgcaca
     1201 atccggacga cggataaccg gtgaaaacgg gaaatgcccg ggcggtgtcg tctgtgcctg
     1261 tttggacaag cattcttgag ggcggtaaaa tcaaggtttg cccgaaaagg gcagggcgat
     1321 atgccgcgct tggattttgc gcgcggtttt tcgcgttgga agcaatcatg tctgtttgaa
     1381 tatttaatgc cgtctgaaac gtccggacgc acccccgttc cttatgaaaa aaccgaccga
     1441 taccctaccc gtcaatctgc aacgccgccg cctgctgtgt gccgccggcg cgctgttgat
     1501 cagcccgctg gcgcacgccg gcgcgcaacg tgaagaaacg cttgccgacg atgtggcttc
     1561 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgttcg acaatccgaa
     1621 agagggcgaa cgttggttgt ccgcgatgtc ggcacgtttg gcaagattcg tccccgacga
     1681 gggggagcgg cgcaggctgc tggtcaatat ccaatacgaa agcagccggg ccggtttgga
     1741 tacgcagatt gtgttggggc tgattgaagt ggaaagcgcg ttccggcagt atgcgataag
     1801 cggggtcggc gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
     1861 accggcgcac aacctgttcg atatccgtac caacctgcgt tacggctgca ccatcctgcg
     1921 ccattaccga aaccttgaaa agggcgacat cgtccgcgca cttgcccgct tcaacggcag
     1981 cctcggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     2041 gcgttgattt tgaacccgtg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     2101 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aattatttat
     2161 ggcaagagac aaccgcatcc aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc
     2221 cggcgtgtac gcgctgcgta tgctgggtat gttcctcgtg ctgcccgttt tggcaatata
     2281 tgccgcctcg ctgcccggtg cggaagacaa taaaacgctg gtcgggctgg caatgggcat
     2341 ttacgggctg acgcaggcat tgctgcaact gcctttgggt atcgcttccg acaagttcgg
     2401 gcgcaagaaa accatttatg tcgggctggt cgtgtttgcc gccggcagct tccttgccgc
     2461 cgccgccgat acgctgccca tgctggttgc cgcgcgcgcc atacagggcg cgggggcggt
     2521 cagtgctgcc gtaaccgcgc tgcttgccga cttgacgcgc gacggcgtgc gtacccgcgc
     2581 gatggcgatg atcggtttga gtatcggtct gacgttttcg gtcagcctgg tggttgcgcc
     2641 gatgattgcc gatgtggccg gcgttcgcgg actgtttatg ctgaccggca ttctgaccgt
     2701 catcagcatc ggcgtagtgg cgtggatgac tcctgatccc gaagtttcca agctgcatga
     2761 ggacacgcag gcgcagcctt cgcgcatagg cgaagttttg aaaaaccgta ggctgctgac
     2821 gctcgatttc ggcattttcg ccctgcacgc cgcgcaaatg gcattgttta ccgcgctgcc
     2881 tttcgcgatg acccggctcg gtttggaaaa aatccagcat tggaaagtat atctgccctc
     2941 gaccattacg ggcttggtgg tgatggttcc gctgattatc gtcggcgaaa cgcgcaacaa
     3001 gctcaaacag gtttttgttt tgggcatcgt ctgcatcgcg gcggcgcagc ttggtttgct
     3061 gtccggaatg cactcgattt ggctgattac cgcttatctg gttgtttact ttatcggttt
     3121 taatgtgttg gaagcgagcc tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa
     3181 gggtacggcg atgggcgtgt acaacacgat gcagtcgctc ggactgtttg ccggcggcgc
     3241 ggcaggaggc ttgctgtttc aaaaatacgg ctttgccggc gtgtttgcct tttgcagcat
     3301 attgatgctg ctgtggctgg tgattgccgt tttatcgccc gcgcccaagc ccgtcaaaaa
     3361 cctcagttac cctgtcggcg gcgtgtggca gggcaatcgg gacggtttgc aacgcgccct
     3421 gttgcagttg gaaggcgtgg aagacatcgg tttcagtttc gacgggcaga ccgtctatct
     3481 taaagtgttg cagaagggtt tcgatcaggc tgccgctgaa aaaatcatta caggagtttg
     3541 aaaaatgtca ttgaacaaag tcatccttat cggccgcctc ggacgcgatc ccgaagtgcg
     3601 ctatatgccc aacggcgagg cagtgtgtaa tttcagcgtc gccaccagcg aaacttggaa
     3661 cgaccgcaac ggccagcgcg tagagcgcac cgaatggcac aacatcacga tgtaccgcaa
     3721 actggcggaa attgccgggc aatacctcaa aaaaggcggg ctggtttatc tggaaggcag
     3781 aatccaaagc cgcaaatacc aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt
     3841 cgccaatgaa atgaaaatgt tgggcgggcg caatgaaaac agcggcggcg cgccttacga
     3901 tgaaggttac ggtcagagtc aggaggctta ccaacgcccc gctcagcaaa gccggcagcc
     3961 cgctcccgac gcgccgtccc atccccaaga agcacccgcc gcgccgcgcc gccaacccgt
     4021 gccggccgcc gccccggtcg aggacattga cgacgacatc ccgttctgaa ttttacggcc
     4081 ggacatcctc gcggagggaa gtcataaagg acggaaaaac cttaaacctt acggggcaag
     4141 gtttctcttt tttgcatttg ggccggcttg ttgccgcccc ctgcgttggg cgtaacgttg
     4201 cgattgcgcc gttcgggcgt tttgaggcaa gcgatgcctg ccgcactccc ccgctgtttt
     4261 cagacggcat tttaaaattg aggcggtgtt ttcaaatagt gcgaagcaat agtcgggctt
     4321 cagccagttt gtttgaaatc aaagggttgg aatcaaacgc caaacgcttg aaccatcatt
     4381 gcccgatgcg gcaaaccggc atcgtccggc aatacggttg tttaaaaata gggaaatcag
     4441 gatggaaaac caaaggccgc tcctaggctt cgcgttggca cttttggcgg cgatgacgtg
     4501 ggggatgctg ccgattgccg tgcggcaggt attgaagttt gtcgatgcgc cgacgctggt
     4561 gtgggtgcgt tttacagtgg cggcggcggt attgtttgtt ttgctggcat tgggcgggcg
     4621 gctgccgaag cggcgggatt tttcttggca ttcattcagg ctgctgctgc tcggcgtgac
     4681 gggcatttcg gcaaactttg tgctgattgc ccaagggctg cattatattt cgccgaccac
     4741 gacgcaggtt ttgtggcaga tttcgccgtt tacgatgatt gttgtcggcg tgttggtgtt
     4801 taaagaccgg atgactgccg cgcagaaaat cggtttggtt ttgctgcttg tcggtttgct
     4861 tatgtttttt aacgacaaat tcggcgagtt gtcgggtttg ggcgcgtatg cgaagggcgt
     4921 gttgctgtgt gcggcaggca gtatggcctg ggtgtgttat gccgtggcgc aaaagctgct
     4981 gtcggcgcaa ttcgggccgc aacagattct gctgttgatt tatgcggcaa gtgccgccgt
     5041 gttcctgccg tttgccgaac cggcacacat cggaagtttg gacggtacgt tggcgtgggt
     5101 ttgttttgtg tattgctgct tgaatacgtt aatcggttac ggctcgttcg gcgaggcgtt
     5161 gaaacattgg gaggcttcca aagtcagcgc ggtaacaacc ttgctccccg tgtttaccgt
     5221 aatattttct ttgctcgggc attatgtgat gcctgatact tttgccgcgc cggatatgaa
     5281 cggtttgggt tatgtcggcg cactcgtcgt ggtcgggggt gcggttacgg cggcggtggg
     5341 ggacaggctg ttcaaacgcc gctagttcgc aggcaacgga aaatgccgtc tgaacgaggc
     5401 ttcagacggc attttatttg agggaaggat tagcgcggat gaaccatatc ggcagggacg
     5461 accagttcgt caaactcttc gcccgtcagc aagcccaacc caacggcagt ttcgcgcagc
     5521 gatttatcgt ttttgtaggc ggttttggcg actttggcgg cgttttcgta gccgattttg
     5581 cggtttaacg cagtaaccag catcagggaa tggtgcagga aatagtcgat tttttccggt
     5641 acgggttcga tgccggcggc gcagttttcg ttgaagctgt tgcacgcgtc gcccaagagg
     5701 cggatggatt gcaagaggtt gtaggcgatg acgggcatat agacgttcag ctcgaaattg
     5761 cccgacgcgc ccgccatacc gatggtaacg tcgttgccga acacttggca gcacaccatc
     5821 gtcatcgctt cgcattgggt cgggttgact ttgcccggca taatggacga acccggttcg
     5881 ttttcgggga ttttgatttc gcccaagccg cagcgcgggc cgcttgccag ccagcggatg
     5941 tcgttggcga ttttgttcag gcttgccgcc agcgttttca atgcgcccga agcggcaacg
     6001 gcggcatcgc gtccgcccaa ggcttcaaat ttgttcggcg cgctgacaaa cggcaggccg
     6061 gacagttcgg cgagtttggc ggcggctatt tcagcgtatt cgggatggct gttcaaaccc
     6121 gtaccgaccg ccgttccacc caaggcgagt tcgtacaaac ctttgagcgc gtcgttcaga
     6181 cggcctaagc tgtggtcgag ttgggaaacg tagccggaaa attcttgtcc caaagtcagc
     6241 ggcgtcgcat cctgcaagtg ggtacggccg attttgacga tgggggcgaa ggcttgggct
     6301 tttttgtcca atgtatcgcg caaggctttt accgccggaa taaggtggcg gttgatttca
     6361 atcgcggcgg cgacgtggat ggcggtcggg aacgcgtcgt tggtcgattg cgcgtggttc
     6421 acatggtcgt tggggtggac gggccggtac gccgccaaac ccgtacctgc aatttcgttg
     6481 gcgcggtttg ccagcacttc gttcatgttc atattggact gcgtaccgga gccggtctgc
     6541 caaaccacta aggggaactg cccgtcgagc ctgccgttca gtacatcatc cgccgcctgc
     6601 gtaatcaaat ccgcctgttc gggcttaatc ctgccgaggg aaacattggt cgcggcggcg
     6661 gcttttttca ccaacgccaa agcgtaaatc agcggctgcg gcagggtttc gccgccgatt
     6721 ttgaaattgt tgcggctgcg ctgggtctgc gcgccccaat aggcttcgga cgggacttcg
     6781 acattgccca tcgtgtcgtg ttcggtgcgg gtgttcatac gtttctcctt ttgaaatgtg
     6841 aataagagtg attcgcaaat attataatgg agattggcgg aatgaggaag catcaaggcg
     6901 ggagggcaaa aaaatgccgt ctgaaaagca ttcagacggc atttgatgcg gcggctgagg
     6961 tttacagcac ggatttcacc gtatcaacca cattgtccac ggtaaagccg aatgctttga
     7021 acagcagatc ggcaggggcg gattcgccga agcggttgat gccgacgact gcgccgttca
     7081 gtccgacata tttgtaccag ccgttggtgt gtccggcttc tacggcgatg cgcggcaggc
     7141 cttccggtag gacggcggct tgataggcgg cgtcttggcg gtcgaagacg ctggtggacg
     7201 gcatggaaac gacgcgcacg gcaatacctt gtcctgccaa tactttttgc gcttctacag
     7261 ccaatcctac ctcggaaccg gtggcaatga tgacggcttg ggcgttgcct tgggcttcac
     7321 tgattacgta agcaccgcgt ttgatgtcgt tcagttgttg ctcgctgcgt gcttggaatt
     7381 tcaggttttg acggctgaaa atcaggcagg acgggtggtc ttcggctttt gccgcttctg
     7441 cccaagcgac cagagattct gcggtgtcgc acggccgcca tacgtccata ttcggaatca
     7501 ggcgcagggt ggcggtttgc tcgataggct ggtgggttgg gccgtcctcg cccaaaccga
     7561 tggaatcgtg ggtgaatacg aagacggggt tgattttcat cagtgccgcc atgcgtaagg
     7621 cgttgcgttc gtattcgctg aacatcagga aagtcgcgcc gaagggtttt acaccgccgt
     7681 gcaataccaa accgttcata atcgcaccca taccgaactc gcgcacgccg tagtggatgt
     7741 agttgccgcc tttgtcgcgg gtaacggaga cgctgtttga ccagtcggtc aggttggacg
     7801 gggtcaggtc ggcagaaccg cccaccagtt cgggcagctc ttttgccaag atttcgatgc
     7861 tgttttggct ggctttgcgg gtggcgacgg tttccgcttt ggcgcacact tctttcaatg
     7921 ccgtctgaac gtactcgtca aagttttccg gcagcttttt atccatgcgg cgcacgaatt
     7981 ctgcggcttc ggcaggatat ttggcttgat attgcgcgaa cagttcgttc catccggctt
     8041 ccagtttcgc gcctttttct ttggcattcc acgcatcgta aatttcttgc gggatttcaa
     8101 acgcaggata agcccaaccc aaatgtttgc gcgtggcttc gatttcgtcc gcacccaaag
     8161 gcgcgccgtg ggttttgtgg ctgccttctt tgttggcgct gcctttgccg attaaggttt
     8221 tgcagcagat gatggacggc ttgccggttt cggcgcgggc ggcttcgatg gccgtctgaa
     8281 tggcggcggt gtcgtggccg tttacgttgg gaacgacgtg ccagccgtag ctttcaaagc
     8341 gttgcgggat gttttcggta aaccagccgt ccactttgcc gtcgatggag atattgttat
     8401 cgtcatacaa aacaatcagt ttgcccaagc ccaaggtgcc ggcgagtgag caggcttcgt
     8461 gcgatacgcc ttccatcagg cagccgtcgc ccataaagac gtaagtgtag tgatcgacga
     8521 tgttcaaacc gtctttatta aattcggcgg caaggatttt ttctgccaat gccatgccca
     8581 ctgcgttggc aataccttgc cccaacgggc cggtcgtggt ttccacgccg tcggtgtagc
     8641 cgtattcggg atggccgggg gttttgctgt gcagctggcg gaagtttttc agatcttcaa
     8701 tgctcaggtt gtagccggtc aggtgcagca ggctgtacaa cagcatagac gcatgaccgt
     8761 tggagaggac gaagcggtcg cggttgtaga atttgggatt ggcgggattg tgattgagga
     8821 atttcgtcca caatgtttcc gccatttccg ccatacccat aggcgcgccg gggtggccgg
     8881 aattggcttt ttgaacggca tcggccgaga ggaagcggat tacgtttgcc agttgagaca
     8941 ttttgtattt tccttgctgg tgtttcggat aagtggataa tcggaaggcg ttgattatcg
     9001 cccgattcgc ttatgctttc aagaaaaggg cggacgcggg ggaaggcggc ggcagggggt
     9061 gggcggtggg gaaggatttt cgatttgcgg gcgaagcctg ccattattcc ttttgaaata
     9121 aaacgttata gattgtgtgc cggattgttg atagcgtttg tttatgagct tgcgccggcg
     9181 gttctgccga taagggggtg tctgtttttt tagtctttgt tttttaagag tattttaatt
     9241 ttgttcgggc ggtgcggtaa aatcagggcg ttttgatgcg gatgggaaat gtattcgccc
     9301 aatacatccg gacggcgcat aaagttgtac aatagcggga atatattttg ggaaaagcgg
     9361 attttctcaa aacttgaaac acaatgcctt aaaaaaacaa gaaaaatgac gtctgaaaag
     9421 caaaacggcg gcggtaacgg gcaaaccatc aatcaaaaat ctaaggaatg cagaatgacc
     9481 acggaaaacc aagccggcag tccggcatcc ggaatcggca cgtccgaaca aacaaaagcc
     9541 gcgccgaaag tgaaaaaaac gttcgatccg cgtgccagcg tgattcaaat ccatcccgaa
     9601 ggcgaacgca tccatcccaa aaaggcggaa ggacggttcg ccaaactgcg tatcgccgcc
     9661 gtattggcga cccagtttgt gttttacgtc attccgtggt tcaattggag cggaaggcag
     9721 gccgtcgttt tcaatatccc cgaacggcat ttcttcattt tcggattgtc gttgggggtg
     9781 ggcgatttga tttaccttgc cctgctgctg atgatttgcg ccttcgggct gttttggtgg
     9841 acgacgattg ccggccgttt gtggtgcggc tattcctgcc cgcaaacggt ttacaccgaa
     9901 attatgctgt ggatcgacaa cctggtcgaa ggcgatagaa acaaacgcct gaagctggaa
     9961 aaatcgccgt ggaatttcac caaaatccgc atcaaagcca ccaaatacct gttgattttc
    10021 cttgtctgcg cgtggacggg catcacgttt gccggctggt ttgtccccat ccgccagttt
    10081 gttcccgatt tattcaccgg agcagcaggc ggcggcgcga tgtttgccgc agcgttttac
    10141 ggctttatga ccttcttctt cgcccatatt atgcgcgaaa aagtgtgcct gcatatgtgt
    10201 ccgtacgcac gtttccaaag cgcgatgttc gataaggaca cgctgattgt ttcttatgac
    10261 gcggagcgcg gcgaaccgcg cggcgcccgc aagaaaacgg tcaacaagga agaggcgggt
    10321 ttgggcgact gcatcaactg tgcgatgtgc gtccaagtct gccccgtcgg catcgacatc
    10381 cgcaacggcc tgcaatacca atgtatcggc tgcgccgcct gtatcgacgc gtgcgatgag
    10441 attatggaca aaatgggtta tccgagcggt ttgatacgct atacgaccga aagcgcgctg
    10501 gaacacgaat atgccgaaaa agacattaaa aaacggctgc tcagaccgcg cgtggcaggt
    10561 tacggcgcgg tgttggcagt ggttgtcgcc gccttcctgg tcggtttgtc cacgcgcaaa
    10621 atggtcgaag tcgatattct gaaagaccgt ggcgtaatgg tgcgcgaaaa cgccaaaggt
    10681 tggctggaaa acgcatatag cctgcgtatc atcaacaaca gcgaaaaaga acagctgatt
    10741 accgcaagtg tcaaaggatt tgacgaaatc gccctgaccg ggctgcctga aggcggtata
    10801 aaagtggctc cgcgcgaaac aataaccctt cccgtccaag tgtccaccat tccggaatac
    10861 gcggacaaag gcagtcatcc tatcgagttt atcttccaat accgggaaag cggtgcatca
    10921 gacggcaagc cggttgtttt ggaagaagac gcaaccttta tcggagaata accgtgtctc
    10981 aaaacaatcc aatcaagcct tggtacaaac acgtctggcc gtgggtcttg atggcggggc
    11041 cgatttttgt cgtcatcgcc agcgtcgcta tgttttttgt cgcgcagcag cacgcgacag
    11101 atttggttac ggacgattat tataaggatg gcaagcatat cgacatccag cttcatcggg
    11161 atgaagaagc cgtcagacgg catatcgggg tgcaggtcct catttctccc gatatgaatg
    11221 cggcaaaagt gtttgtcggc ggcgagtttg acggcaaaca gcctttgaac ctgctgctga
    11281 tgcacccgac ccgcaaggcg gacgatcaaa ccgtcgccct caagcccgtc ggcagcgcgc
    11341 agaacggcag ggcggaatat gaggcggtgt tcaaaaccct tccgccggcc aaccactggt
    11401 atgtgcgcgt ggaggacgcg gcaggcgtgt ggcgcgtcga gaacaaatgg attaccagcc
    11461 agggcaatgc ggtcgatttg accccgatgg acaaactttt caataatgca ggaagcaaat
    11521 aaaaaagccg tgttttcatt gcattggttt gttatatgca atgtcgggca ggcattgttt
    11581 gattattcgg cgtgatggtc tgatttgtcg gacgaaaata aatgtatctc atcccgttta
    11641 ttttttaaca tcattccgaa aggcagcctg aaaaatgccg tctgaaaggc ttcagacggc
    11701 atttgtgttg tatgtccgac ataatgcgga aggcattttg ttcatataaa aacgttttta
    11761 tttctctccg ttttttcgac actgttccgg aacacaacct gaaaaacgcc gtctgaaagc
    11821 ccttcggacg gcattttgtt tgcaaatcaa atcctacctg atgtcagcgt ccgagcgttg
    11881 caacaggttc gctccccgtc agcagatatt gtatcgtttc gcggacggtg cggatggcat
    11941 taccgaaggg aagctggtct tttcccctga aaaacaaccc cttatcaact tctccacgga
    12001 atgcggcagc aagctggata tcaatacaga attgccctgc tttggaaagc ccgtcgcgca
    12061 gaccgcagct ggtcaggcag tttaaacctt gggtacagcg gcgcgggtcg gctttggcgt
    12121 ttgcctgaag tttgccttcg cgcctgatat agctgtctag gaatttggtg cggataccgc
    12181 gcgccggcaa accggcaaca gacataaatt cgactacttt ttcagtttcc gcactggcga
    12241 gcgttttttt aaagttgagg tgtgcatctc cttcttcggt aacggcaaaa gccgtaccga
    12301 tttgaacggc ggaggctccc cagttcttta gggcggtttt gactttttca aaatttgcca
    12361 tgcctcccgc aagaataagc gggatttttt ctccctccag ccccaaattt ttaaacactt
    12421 caaacgtttc ctcaatcacg cgtttgaagt cgaatttggc atcgtttacg ccttcaacgg
    12481 ttgatgcacc caaatgtccg gccgcatggg caggatgttc gactacaatc gcatcgggca
    12541 atatgccttt tttcatccaa cgtttcaaga cgatattaat accgcgcgat tcggacagaa
    12601 tcggcagcag cgcgacatcc ttatgatagc cctcggttat ttccggcagg tctaaaggca
    12661 ggccggcacc catcacgacc gcatccgccc ctgattcgca agcctggcgg acatacgcgg
    12721 cgtggtcttt gaccgccttc atcacgttga ccgcaatcag tccttttccc tctgaagcac
    12781 ttttggcttt ttggatttcc ctgtctaatg cggtacagtt caaagacgta tatttctctt
    12841 cactcggatt gatttgggat tcggcgagca ggtcttcgtg aaggtggcgc aaatctacac
    12901 tggcaatcgt tccgatgccg ttttcacgcg ccaccgcgct ggataaaccc gatgcggaaa
    12961 caccgacccc cataccgcct tgcacgatgg gaataaggga ttttccacga ataatcaaag
    13021 ggtcaaaaat attctgcatc agtttctccg aatatacgaa tcagtcaggc tcatgaaaaa
    13081 tggttttaat tgttttagaa ccattactca aatttggtat tatactctaa atattgcagt
    13141 ggtaaacaga aaagcagaag gagctttgga atatgctgtt atatctgtac caatcggaca
    13201 aaatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg tactatttgt
    13261 actgtctggg gcttcgtcgc attgtcctga tttaaattta atccactata ttgaacggac
    13321 accgcatccg gttacgggat atcggtttgc atggcaaaag tttgaatatg attcgattac
    13381 gtctgagatt ctgatgaagt gggtaggtaa agatatggta acgaattgcc gaggcaataa
    13441 aaatccccga gggattattt cggggatttg gaatctggca cgcccacggg gaatcgaacc
    13501 ccggttaccg ccgtgaaagg gcgatgtcct aaccgctaga cgatgggcgc
//
LOCUS       DAXDZG010000062        13535 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXDZG010000062 DAXDZG010000000
VERSION     DAXDZG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.062"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..668
                     /locus_tag="ACFB78_001589"
                     /pseudo
     CDS             1..668
                     /locus_tag="ACFB78_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248723.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            843..1709
                     /locus_tag="ACFB78_001590"
     CDS             843..1709
                     /locus_tag="ACFB78_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003788484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1269550.1"
                     /translation="MVRTAASSSVKKLADEKKEICQQQFFSVISKRDKLLYIVKSDYS
                     KDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLEAEGNVELKNGKKPESLIETIIKLA
                     TNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERMKKVIDGEQGGISK
                     AVNWQGGGEFVYAELSPFNETAKQQILACENSDGIKTLFEGLCERHFLKYNVSVNEFS
                     QIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLSEMDDEQFAGCLNDDDKALSRAFYQ
                     SVKHQAEKKDGE"
     gene            1699..4473
                     /locus_tag="ACFB78_001591"
     CDS             1699..4473
                     /locus_tag="ACFB78_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC1269551.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENAEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(4622..5794)
                     /locus_tag="ACFB78_001592"
     CDS             complement(4622..5794)
                     /locus_tag="ACFB78_001592"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC1269552.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(5915..6157)
                     /locus_tag="ACFB78_001593"
     CDS             complement(5915..6157)
                     /locus_tag="ACFB78_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269553.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            6144..6590
                     /gene="iscR"
                     /locus_tag="ACFB78_001594"
     CDS             6144..6590
                     /gene="iscR"
                     /locus_tag="ACFB78_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC1269554.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6619..7833
                     /locus_tag="ACFB78_001595"
     CDS             6619..7833
                     /locus_tag="ACFB78_001595"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC1269555.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7818..8129
                     /locus_tag="ACFB78_001596"
     CDS             7818..8129
                     /locus_tag="ACFB78_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269556.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            8435..8929
                     /locus_tag="ACFB78_001597"
     CDS             8435..8929
                     /locus_tag="ACFB78_001597"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC1269557.1"
                     /translation="MQPITGNFDLAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFA
                     NFAFIENESRKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLF
                     MTQLAIKNGFQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQ
                     TTIF"
     gene            8942..9328
                     /gene="iscU"
                     /locus_tag="ACFB78_001598"
     CDS             8942..9328
                     /gene="iscU"
                     /locus_tag="ACFB78_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC1269558.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9415..9735
                     /gene="iscA"
                     /locus_tag="ACFB78_001599"
     CDS             9415..9735
                     /gene="iscA"
                     /locus_tag="ACFB78_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC1269559.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9695..9916)
                     /locus_tag="ACFB78_001600"
     CDS             complement(9695..9916)
                     /locus_tag="ACFB78_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC1269560.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            9998..10498
                     /gene="hscB"
                     /locus_tag="ACFB78_001601"
     CDS             9998..10498
                     /gene="hscB"
                     /locus_tag="ACFB78_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC1269561.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            10596..13346
                     /gene="gyrA"
                     /locus_tag="ACFB78_001602"
     CDS             10596..13346
                     /gene="gyrA"
                     /locus_tag="ACFB78_001602"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC1269562.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYGTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         4203 a         3305 c         2914 g         3113 t
ORIGIN      
        1 ttatacacca aattaaccag aaaaagacaa taaatctttt ttaatcaaac gcttgctttt
       61 gatgaaattg accggctttt tgacgcaaaa gcattctcaa aattctcccg ccacaccgca
      121 gacggcaagc aacccgttgg cgaaatcaaa cgccgttcag acggtacgcc agccgaaaat
      181 ctcattatca aaggcaataa tctgattgcc ctgcattcgc ttgccaagca gtttaaaggc
      241 aaagtaaagt tgatttatat tgatccaccg tataatacag aaaccgatag ctttgcatat
      301 aacgataaat tttcacattc cacttggcta acatttatga aaaatcgctt ggaaattgcg
      361 aaagagttat taaaagatga tgggctaatc tttgttcaat gcgatgataa ggaacaggct
      421 tatttaaaag ttttattaga tgaaactttt acaagagaaa attttataaa ctgcattgct
      481 gtaaaaatgt cagagccatc aggtaacaaa atggcacata catcgcatag actaccaaag
      541 ataaaggaat atattcttat ctataagaat aaaaatataa aattaaaccc tattagagag
      601 caaaaatcag aatgggacaa tgagtacaat attttttaga aaattttaca caagaagata
      661 aaaaatttat tgatttaata gtaaatagcc aaacagaaaa taaagaaata aatggtaata
      721 ctttaaaaga aattgatatt cttttaaaga aaatatctcc tatttcagtt aatcaaaaat
      781 tagcccaatt aaatattaaa gataatgaag ttataaaatg gaaactagat aatgcgtata
      841 gaattgtaag aacagcagct agtagtagcg taaaaaaatt agcagatgaa aaaaaagaaa
      901 tttgccaaca acaatttttt tcagttattt ctaaacgaga taaattattg tatatcgtca
      961 agtcagatta ctcaaaagat gcaaaagcac cacgagtgca ggttttgttt gcagaagatt
     1021 atttgagtat tagtttatgc gatttatgga caaatataaa taccacagga ctagaagcag
     1081 aaggaaatgt agagttaaaa aatggtaaaa agccagaaag tttaattgaa accatcatta
     1141 aactagcaac caacgaaaac gacatcgttc tagactacca cttgggcagt ggcacaaccg
     1201 ccgccgtcgc ccataaaatg aaccgccaat acatcggcat tgaacaaatg gattatattg
     1261 aaacgcttgc cgttgaacgc atgaaaaaag taattgacgg cgagcaaggc ggtatctcaa
     1321 aagctgtgaa ttggcaaggt ggcggcgagt ttgtttatgc cgaactttcc ccatttaacg
     1381 aaaccgcaaa acaacaaatt ttggcgtgcg aaaattcaga cggcatcaaa acgctgtttg
     1441 aaggtttatg cgaacgccat ttcttgaaat acaacgtcag cgtaaatgaa tttagccaaa
     1501 ttattgaaga gcctgaattt caatccttgg cattagacga acaaaaacaa atgatgcttg
     1561 aaatgttgga tttaaatcaa atgtatattt cattatccga aatggacgat gaacaatttg
     1621 caggctgcct gaatgatgat gataaagcgt taagccgtgc gttctatcaa tcagtaaaac
     1681 atcaagcgga gaaaaaagat ggcgaataat aaaacgttgt ttgattgggt ggaagaccgc
     1741 aaatcaatgc tggaagaaat ggaacagacg gattttttcg cgctgcctga atttgtttcc
     1801 aataatttaa aatatccgtt ttttgaatgg caaaaatcgg ctttagaaaa ttttgtgatt
     1861 tttgaccgca cttcaaaatt aaaggatttc cccgacatca aaaaccgccc tacccatttg
     1921 ctgttcaata tggcaacagg tgcaggcaaa acgatgatga tggcggcatt gattctgtat
     1981 tattttgaga aagggtatcg gcatttttta tttttcgtca ataaaaacaa tatagtatat
     2041 aaaacggaaa ataattttat cgatccgacg catccaaaat ttttatttac cgagaaaatt
     2101 ttgcaaggtg atacggtaat tcccattcgc aaagtggaga catttagcca atattcagac
     2161 ggcattgaaa ttaaatttac cagcattcaa aaactatata acgatattca caccgagcgg
     2221 gaaaatcaaa ccacattggc ggatttgcac gaattaaatc ttgtgatgct gggcgatgaa
     2281 gcacaccatt taaacgcgca aaccaaaggc aaaaaacaag gcgaattaga tttagaaaag
     2341 gaaatgaacg agcgtaccag cgatgccgaa attgaacgca aaggctggga gcatatggtt
     2401 ttggagttgt tactcaataa aaatggcaat cccggccaaa atgtgctgtt ggaatttacc
     2461 gccacactgc ctgaaaatgc tgaagtgcaa caaaaatatg cggataaaat catcacaaaa
     2521 tttggtttaa aagaattttt gcaaaaaggt tataccaaag aaatcaattt ggtatccggt
     2581 acgctgaata agaaagagcg ggtgttgcac gctttattgt ttgcctggta ccgccatcaa
     2641 atcgcgttga aatacggcat tgccaatttc aagcctgtga tgttgtttag aagtaagacg
     2701 attgatgaat caaaagcgga ttatttggca tttttaaatt gggtagaaaa tgtgcaagcg
     2761 gatgattttt cgtttttaac tacattttca acaagcttaa acgatagcaa taacgccaac
     2821 gaacaaggca aaacccgtac cgaacaagcc ctgaaattta tgcaggaaaa taaatttgaa
     2881 tttgtgcatt tggcaaattg gattaaacaa aattatcaaa aacacaatgt cattattacc
     2941 aactccgaaa ccaacaaaaa caaaactgaa aaaaccgaca gcgaaaccga aaaattgctg
     3001 aacaatctgg aagcggctga taatccgatc cgtgccattt ttacggtgga tagattaacc
     3061 gaaggttggg acgttttaaa tttgtttgat attgtgcgtt tgtatgaagg gcaaaacggt
     3121 ggcggttcga ataaaaaatc aggcaaaacg gctgccgcca ccgtatcgga aaagcagttg
     3181 attggtcgcg gcgtgcgtta ttttccattt gcgtttgaag gtaaacagcc gaataaacgc
     3241 aaatttgaca acgatatgca acatgaattg cgtatcttgg aagaattgtt ttattacacg
     3301 cacgatgagc aatcccgcta tattacagaa ctgaaaaacg agttacgaaa agacggttat
     3361 ttgcctgaaa aagacgatga taaggtattg gcaacattta agctcaaatc tgaatttgca
     3421 gataataagg attttagaga gttgttaatt tgggcgaata aaaaaatccc caacccaaac
     3481 gccaaaagca ataatgcaga cagcctgcaa gccaatccgc aaacgctttc attccaagtt
     3541 cacggtaatc aactgttgca ggaaacgcaa tttacagccg atgaaaatga tgaaacagcc
     3601 cgacaaatcg gcacacaaaa taattttact caaaccataa aaatgagtga aatggagcgg
     3661 catattttca ataaagcatt acatatcaaa gggaaaaata gtcaatcttt attccatttt
     3721 gaccgcctgc aaagcaaact cgacattcaa aatcgcaatg aattgcaaaa taaattgttg
     3781 aaagactggc agattgaatt tttgggatta gagcaagata aacaggttcg cccggatgat
     3841 aaacttgcag gctgcctgaa aatcttggaa atggttgaaa aacatctgaa tgaaagtgat
     3901 ataccgttta tcggtacaaa agaatttaca cctaaaaaat tgtgggaaat tttcggcacg
     3961 ccaaaacaaa aatgggtcaa aaaagatgat ataaaaactg ctattgccac gcaaaatgat
     4021 tggtatgtga tggataattt tgccggaacg ggtttggaag aagcgttaat tcaatttatt
     4081 tcagcgcgct tgggcgattt gaaatctcaa tatgacgttc atttaatccg caatgaagaa
     4141 gtgtttaaat tgaataactt tgccgatggc gaaggattta tgccggactt tgttttattg
     4201 ctgaaagata aacaaaaatc ttcttccgac agcgtggacg actttttgca ttaccaaatt
     4261 ttcattgaac cgaaaggcgg gcatttagtg gaaaatgatt cgtggaaaga agctttttta
     4321 aaatcaatca cagtggaata tgggagggat aagatcctgc aaaaaaatac accgcattat
     4381 cgtttgatcg gtttgccgtt ttttactgat catcagaaaa atggacaatt tacagagtta
     4441 ttccctttag gtgcggcatc gcttgaaaaa tagagtagtg cattgcaggc agccccgttc
     4501 ggcaaaactt cctttacaaa agggcgtttt gtcagatatt taatcaacac attattaaaa
     4561 tacaaccaaa ttttaatgcc gtccgaacca tgtattcaga cggcatcgta tttttcagta
     4621 tctaaaccgt ttccctgccc caatccttac tcctcaaaat cgaggcatcg acatcttgaa
     4681 tatcgcggtg tcccgtaaac gccatcgaca catccatttc cttgtacaaa atctccagcg
     4741 cgcgcgttac gccttcttcg ccatacgcac ccaaaccata caggaacgcc cgacctatca
     4801 tcgtgccttt cgcacccaaa gcccacgcct tcaaaatatc ctggccgctg cggataccgc
     4861 tgtccatcca aacttcgatg tcgctgccga ctgcggaaac aatatcgggc aaggccttga
     4921 tggcggacac agtatcgtcg agctggcggc cgccgtggtt ggaaacgacc aatgcatccg
     4981 caccgctttt cgccgccttt tccgcgtctt cgggttccat aatgcctttg ataatcagct
     5041 tgccgcccca caaatcttta atccgcgcca cgtcgtccca gcttaagcgc gggtcgaact
     5101 gttccgccgt ccacgaagaa agtgaagaca aatcgccgac gtttttggcg tgtccgacga
     5161 tattgcggaa cgtgcggcgt tccgtgttca gcattttcat gcaccattcg ggcttggtcg
     5221 ccagattgat taaattggcg atggtcggtt tcggcggcgc ggacaggccg tttttgatgt
     5281 ctttgtggcg ttgacccaaa acctgcaaat cggcagtcaa taccaatgcc gaacatttgg
     5341 catctttcgc acgcttaatc aggttttcca taaattcgcg gtcgcgcatc acataaagct
     5401 gaaaccaaaa cggcgcgctg gtgttctcgg caacgtcttc aatcgagcag atggacatgg
     5461 tggacaacgt aaacggaata ccgaacttct ccgccgcacg cgccgccaaa atttcgccgt
     5521 ctgcgtgtgc catgccggta aaacccgtcg gcgcaatcgc caccggcatt ttcacatccc
     5581 ccccgatcat tttggtttcg aggctgcgtc cctccatatt gaccaatacc ttttgtcgga
     5641 agcggatatc tttgaaatcc gaagtattct ccctataagt cgtttccgtc cacgaacccg
     5701 aatcgatgta atcgtaaaac atacgcggca ttttgcgctt ggcaacgcgg cgcaagtctt
     5761 cgatgcaggt cattttgctc aaatcacgtt tcatcttgtt tttccctgtt taacagcctg
     5821 atggaatatg ccgtctgaaa ctgcttgagc cttcaaacgg catttcgggc aatacccaaa
     5881 taaatcacta agatttagat aatctatatc aatattaaaa ataaatcaaa tcgttttgaa
     5941 accgcttgcc ggcaaaacgc cccaaccgcc gctcctcaaa aaacataact aactgattca
     6001 aaaaaccaaa caaataaagc aaaatcagac tataccgcca tttaaacaaa caatcggcaa
     6061 cagctccccc atacttgact gaaacactca gatattggac aattccgccc actataaaaa
     6121 agccgacacg ggcaaccacc accatgagac tgaccaccaa agggcgtttc gccgttaccg
     6181 ctatgctgga tttggcgatg aacgcgcaaa ccggcgccgt caaactcagt gccatcagcg
     6241 aacgccaaaa tatatccctc tcctatctcg agcaattgtt cggcaaactc cgccgcgccg
     6301 gactggttga aagcctgcgc gggcccggcg gcggctacat cctcgccgcc cctcccgcac
     6361 aaatcaacat cgcccaaatc atttccgccg ccgaagaccg tctggacgcg acccaatgcg
     6421 gcagcaaagc caactgccac cacggcgcgc cctgcctgac gcacgacctt tgggagaatt
     6481 taaacaaaac catcaacgac tatctcggca gcgttaccct gcaaagcatc atcgaacaga
     6541 aaaacaacgg cgacggcagc cgcgtcgtcc aatttacaca catccattaa ataacacccg
     6601 aaaaagaaag agcaaaccat gaccgtcaaa acccccgttt acctcgacta cgccgccacc
     6661 acacccgttg acaaacgcgt tgccgaaaaa atgattccct atctgactga aaccttcggc
     6721 aaccccgcct ccaacagcca cgcattcggc tggacggcag aagaagccgt cgaaaaagcc
     6781 cgcgccgaca tcgccgccct gattaacgcc gaccccaaag aaatcgtctt caccagcggc
     6841 gcgaccgagt ccgacaacct cgccatcaaa ggtgcggcaa acttctacaa aaccaaaggc
     6901 aaacacctca tcaccgtcaa aaccgaacac aaagccgtgc tcgacacgat gcgcgaactc
     6961 gaacgccaag gctttgaagt tacctacctc ggcgtgcagg aaaacggttt gattgatttg
     7021 gaagaactca aagccgccat ccgcgacgac accatcctga tttccataat gtgggtgaac
     7081 aacgaaatcg gcgtggtgca aaacattccc gccatcggcg aaatctgccg cgaacgcaaa
     7141 atcgccttcc acgtcgatgc cgcccaagcc tgcggcaaag tgcctgtcga tgtcgaagcc
     7201 gccaaaatcg acttgctctc gatgtccgcg cacaaagtgt acggccccaa aggcatcggc
     7261 gcgctgtacg tccgccgcaa accgcgcgtc cgcctcgaag cccaaatgca cggtggcggt
     7321 cacgagcgcg gtttccgttc cggcacattg ccgacccatc aaatcgtcgg catgggcgag
     7381 gccttccgca tcgccaaaga agaattggca caagacacag cgcactacct gaaactgcgc
     7441 gacatcttcc tcaaaggcat cgaaggcatc gaagaagtct atatcaacgg cgacctcgaa
     7501 caccgcgccc cgaacaacct gaacgtcagc ttcaacttcg tcgaaggcga aagcctgatt
     7561 atggcggtga aagaactcgc cgtatccagc ggatccgcct gcacctccgc ctcgctcgaa
     7621 cccagctacg tcctgcgcgc gcttggccgc aacgacgaac tggcgcactc atccctgcgc
     7681 atcaccttcg gccgcatgac caccgaagaa gaagtgcaat tcgcggcaga actgatcaaa
     7741 tccaaaatcg gcaaactgcg cgaactgtcg ccgctgtggg aaatgttcaa agacggtatt
     7801 gatctgaact cgattgaatg ggcggcgcat taagattgat gtcaaacaaa ggctgtctga
     7861 gaaagaatac catgaaagtt aaaattatta atttgccaaa tggttataaa cgtattattt
     7921 atggaaaata ttttgaacaa tttgacttag attatgaaca agatttagac gtattaaaaa
     7981 aagatattga atttgcttta tctgttattg aatacaatcg atctatattc aaaaaattct
     8041 cttccttatt tgagaataaa atcatttttg tttatcaagg aggacatcat cttgatatta
     8101 ttgatcgtga taaaggaagt ttgaaatagt taactattga agatcattgg acaaaagatg
     8161 ttgatgatat cactggttca tggaaaatgg agaatttaga aattagccga caaactcaat
     8221 atgcaatgca acttctaaag attggtaaaa caaatattga gaaagaaata agaaaaatta
     8281 aacaaaggtt ctaaaaaaat gagtaaatac ggtggtgaag acgtaatcta tatgaatgat
     8341 ggtgtattta gaaataaatt agccattcaa gatccactta aatttaaaaa aggtcgaaag
     8401 agacatttct gctatttccg cccaaaatct gcttttgcaa cccattacag gcaatttcga
     8461 tttggcgcat ttacagacca ttcatcgcga actgtttggc aatgtttatg attgggcagg
     8521 aaaaatccgg agggttgata tttctaaagg taatacccga tttgccaatt ttgcttttat
     8581 cgaaaacgaa agccgtaaac ttttggagaa gctgaaaaac gaaaactatt tgcgggtgtt
     8641 ggataaagat aaattcgccg agcgtgcagc ctattatttg gacgagttga acgtcctaca
     8701 tccttttcgt gaaggaaacg gcagaacatt gcgcctgttt atgacacaat tggcaataaa
     8761 aaacggtttt cagatacatt ggcaaaatat ctctgccgaa caaatgattc aagcctgtat
     8821 tcaggcatac catgccgata gcagcttatt ggcacgccta attattagac aatttagaac
     8881 aatcgtcttt ttcagacgac taaaacatca acaaaccacg atattttaag gaaaccacat
     8941 catggcatac agcgataaag taatcgacca ctacgaaaat ccccgcaacg tcggcacttt
     9001 cgacaaaaac gacgagtccg tcggcaccgg catggtcggc gcgcccgcct gcggcgacgt
     9061 gatgcgcctg caaatcaaag tgaacgatga aggcatcatc gaagatgcga aattcaaaac
     9121 ttacggctgc ggttccgcca tcgcttcgtc cagcctgatt accgagtggg tcaaaggcaa
     9181 aagtctggat gacgcgctgg caatcaaaaa cagcgaaatc gccgaagaac tggaattgcc
     9241 gccggtaaaa atccactgct ccatcttggc tgaagatgcg gtaaaagcgg ccgttgccga
     9301 ctaccgcaaa cgtcaggaaa acagataaag cccttcagac ggcatcgtcc cacaatgccg
     9361 tccgaaccac ccgccgcttc ggatccgtcc ggggcggtac aacaaggaag aaatatgatt
     9421 acccttaccg agaatgccgc aaaacacatc aatgactatc tcgccaaacg cggcaaaggc
     9481 ttgggcgtac gcttgggtgt aaaaaccagc ggctgctcgg ggatggcgta caaccttgaa
     9541 tttgtcgatg aagccaacgg cgacgacctg attttcgaag gacacggcgc gcgcatttat
     9601 atcgacccga aaagcttggt ttatctggac ggcacacaag tcgattacac caaagaagat
     9661 ttgcaggaag gtttcaaatt tgaaaacccc aatgtcaaag actcctgcgg ctgcggcgag
     9721 agcttccacg tttaaggcat aaaaacggcg ggacagtatc aaaaccgtcc cgccgttttt
     9781 tcgcttcctg cctgttatag ctgcctttgc ctttcctttt ccgttccacc ttgtgccgga
     9841 acaaatcgga ttttactaag gcttttaaag cattgtcgcg tattttgcct ttattgtgct
     9901 gcgctttgcc gcccatattc agtcctttcg tttaagaagc cgcagattat aaggcaaaaa
     9961 cagttttctg ccaaaatctt acatttatca tcctactatg tcccaatatt tcaccctctt
    10021 ccggattgaa cccgctttcg atatcggcac cgaaaacttg gaacaaacct accgcgcctt
    10081 ggccgcccgt ttccatcccg acaaattcgc ttccgcttcc gctttcgagc agaaacaggc
    10141 ggttatgatg tcttccacca tcaacgatgc ctaccgcacc ttgaaaaatc ccatcgaccg
    10201 cgccgcgtac ctgctgaaaa catcgggtat cgatgccgat gcgccggaac atacctcttt
    10261 cgcccccgac ttcctcatgc agcaaatgga atggcgcgaa acgctgatgg aggcacgggc
    10321 aggcaacaac cttgaatcct tgaaaaatct cgataacgaa atccgcgccg aacaagaaaa
    10381 actgttctgc ggtctgaaac agtcattcgc gcgccaagac tgcgacaccg ccgcacaaca
    10441 agtccgccaa ggcaggtttc tcgacaaact ccgccacgaa atttcctccg cattataatc
    10501 cgcaccgtgt ttcagacggc ataaccgccg caccgtttca tgtcgaaata tgctaaaata
    10561 agcaacaatt ttttgccata cgaaacattg aaaccatgac cgacgcaacc atccgccacg
    10621 accacaaatt cgccctcgaa accctgcccg tcagccttga agacgaaatg cgcaaaagct
    10681 atctcgacta cgccatgagc gtcattgtcg ggcgcgcgct gccggacgtt cgcgacggcc
    10741 taaagccggt gcaccggcgc gtactgtacg cgatgcacga gctgaaaaat aactggaatg
    10801 ccgcctacaa aaaatcggcg cgcatcgtcg gcgacgtcat cggtaaatac cacccccacg
    10861 gcgatttcgc agtttacggc accatcgtcc gtatggcgca aaatttcgct atgcgttatg
    10921 tgctgataga cggacagggc aacttcggat cggtggacgg gcttgccgcc gcagccatgc
    10981 gctataccga aatccgcatg gcgaaaatct cacatgaaat gctggcagac attgaggaag
    11041 aaaccgttaa tttcggcccg aactacgacg gtagcgaaca cgagccgctt gtactgccga
    11101 cccgtttccc cacactgctc gtcaacggct cgtccggtat cgccgtcggt atggcgacca
    11161 acatcccgcc gcacaacctc accgacacca tcaacgcctg tctgcgtctt ttggacgaac
    11221 ccaaaaccga aatcgacgaa ctgatcgaca ttatccaagc ccccgacttc ccgaccgggg
    11281 caaccatcta cggcttgggc ggcgtgcgcg aaggctataa aacaggccgc ggccgcgtcg
    11341 ttatgcgcgg taagacccat atcgaaccca taggcaaaaa cggcgaacgc gaagccatcg
    11401 ttatcgacga aatcccctat caggtcaaca aagccaagtt ggtcgagaaa atcggcgatt
    11461 tggttcggga aaaaacactg gaaggcattt ccgagctccg cgacgaatcc gacaaatccg
    11521 gtatgcgcgt cgttatcgag ctgaaacgca acgaaaatgc cgaagtcgtc ttaaaccaac
    11581 tctacaaact gactccgctg caagacagtt tcggcatcaa tatggtggtt ttggtcgacg
    11641 gacaaccgcg cctgttaaac ctgaaacaga ttctctccga attcctgcgc caccgccgcg
    11701 aagtcgttac ccgacgtacg cttttccggc tgaagaaggc acgccatgaa gggcatatcg
    11761 ccgaaggcaa agccgtcgca ctgtccaata tcgatgaaat catcaagctc atcaaagaat
    11821 cgcccaacgc ggccgaggcc aaagaaaaac tgcttgcgcg cccttggcgc agcagcctcg
    11881 ttgaagaaat gctgacgcgt tccggtctgg atttggaaat gatgcgtccg gaaggattgg
    11941 ctgcaaacat tggtctgaaa aaacaaggtt attacctgag cgagattcag gcagatgcta
    12001 ttttacgcat gagcctgcga aacctgaccg gcctcgatca gaaagaaatt atcgaaagct
    12061 acaaaaacct gatgggtaaa atcatcgact ttgtggatat cctctccaaa cccgaacgca
    12121 ttacccaaat catccgtgac gaactggaag aaatcaaaac caactatggc gacgaacgcc
    12181 gcagcgaaat caacccgttc ggcggcgaca ttgccgatga agacctgatt ccgcaacgcg
    12241 aaatggtcgt gaccctgacc cacggcggct atataaaaac ccagccgacc accgactatc
    12301 aggctcagcg tcgcggcggg cgcggcaaac aggcggctgc caccaaagac gaagacttta
    12361 tcgaaaccct gtttgttgcc aacacgcatg actatttgat gtgttttacc aacctcggca
    12421 agtgccactg gattaaggtt tacaaactgc ccgaaggcgg acgcaacagc cgcggccgtc
    12481 cgattaacaa cgtcatccag ctggaagaag gcgaaaaagt cagcgcgatt ctggcagtac
    12541 gcgagtttcc cgaagaccaa tacgtcttct tcgccaccgc gcagggaatg gtgaaaaaag
    12601 tccaactttc cgcctttaaa aacgtccgcg cccaaggcat taaagccatc gcactcaaag
    12661 aaggcgacta cctcgtcggc gctgcgcaaa caggcggtgc ggacgacatt atgttgttct
    12721 ccaacttggg caaagccatc cgcttcaacg aatactggga aaaatccggc aacgacgaag
    12781 cggaagatgc cgacatcgaa accgagattt cagacgacct cgaagacgaa accgccgaca
    12841 acgaaaacac cctgccaagc ggcaaaaacg gcgtgcgtcc gtccggtcgc ggcagcggcg
    12901 gtttgcgcgg tatgcgcctg cctgccgacg gcaaaatcgt cagcctgatt accttcgccc
    12961 ctgaaaccga agaaagcggt ttgcaagttt taaccgccac cgccaacgga tacggaaaac
    13021 gcaccccgat tgccgattac agccgcaaaa acaaaggcgg gcaaggcagt attgccatta
    13081 acaccggcga gcgcaacggc gatttggtcg ccgcaacctt ggtcggcgaa accgacgatt
    13141 tgatgctgat taccagcggc ggcgtgctta tccgtaccaa agtcgaacaa atccgcgaaa
    13201 ccggccgcgc cgcagcaggc gtgaaactga ttaacttgga cgaaggcgaa accttggtat
    13261 cgctggaacg tgttgccgaa gacgaatccg aactctccgg cgcttctgta atttccaatg
    13321 taaccgaacc ggaagccgag aactgaaaat catctcccga tgccgtctga agattcagac
    13381 ggcatttatt tttccctcat ccgtcatcca gcttctcaca atatagcgga ttaacaaaaa
    13441 tcaggataag gcgacgaagc cgcagacagt acaaatatag tggattaaat ttaaactagt
    13501 acagcgttgc ctcgccttgc cgtactatag tcaat
//
LOCUS       DAXDZG010000063        13482 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXDZG010000063 DAXDZG010000000
VERSION     DAXDZG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.063"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFB78_001603"
     CDS             complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFB78_001603"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC1269563.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFB78_001604"
     CDS             1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFB78_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC1269564.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1871..2695
                     /locus_tag="ACFB78_001605"
     CDS             1871..2695
                     /locus_tag="ACFB78_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC1269565.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFB78_001606"
     CDS             complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFB78_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC1269566.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4507..5730)
                     /locus_tag="ACFB78_001607"
     CDS             complement(4507..5730)
                     /locus_tag="ACFB78_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC1269567.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6167..6580
                     /locus_tag="ACFB78_001608"
     CDS             6167..6580
                     /locus_tag="ACFB78_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC1269568.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6627..7256
                     /gene="nth"
                     /locus_tag="ACFB78_001609"
     CDS             6627..7256
                     /gene="nth"
                     /locus_tag="ACFB78_001609"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC1269569.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7394..8893
                     /locus_tag="ACFB78_001610"
     CDS             7394..8893
                     /locus_tag="ACFB78_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC1269570.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9096..10616
                     /locus_tag="ACFB78_001611"
     CDS             9096..10616
                     /locus_tag="ACFB78_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC1269571.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            10673..11758
                     /gene="nagZ"
                     /locus_tag="ACFB78_001612"
     CDS             10673..11758
                     /gene="nagZ"
                     /locus_tag="ACFB78_001612"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC1269572.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11983..12059
                     /locus_tag="ACFB78_001613"
     tRNA            11983..12059
                     /locus_tag="ACFB78_001613"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12017..12019,aa:Pro,seq:cgg)
     gene            complement(12395..13033)
                     /locus_tag="ACFB78_001614"
     CDS             complement(12395..13033)
                     /locus_tag="ACFB78_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC1269573.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(13030..13398)
                     /locus_tag="ACFB78_001615"
     CDS             complement(13030..13398)
                     /locus_tag="ACFB78_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC1269574.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
BASE COUNT         3381 a         3841 c         3396 g         2864 t
ORIGIN      
        1 ggtgtggaat ttagggataa tctagtacag ccacaataaa aaatacgaat atcatcattc
       61 tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct ttccgccgtc
      121 attcccgcgc aggcgggaat ctggaaaccc aatgctgcaa gaattgatta aaaacaactg
      181 aaaccgagca aactggattc ccgcctgcgc gggaatgacg aaataaggtg agtaaaactt
      241 attaaataaa ttaattaaaa acaataactt atattatttt ctttaaattt tcgataaaat
      301 caattttccg tattcaatac tgctccaatc aattcctgcg caccgtcgtc cgccagtttt
      361 ttggcaaccg cacgtccaag cgcatcggca tatccggcag gggcttgcgc gtccgcctgc
      421 aacacaatcg atccgtcggg atgcccgacc aatccgcgca agatcagcag cccgttttct
      481 tcagtgcaat acgcggccag cggcacttgg cagcttccgc ccaaagcgcg cgccaaagcg
      541 cgttcggcgg taacgcaggc gtgtgtggta tcgtggttta acggcttcaa gacttcgtac
      601 aaatcttcgc gatgcgtggc aatttcaata cccaatgcgc cttgtccggc ggcaggcagg
      661 ctgtcggatt ccgacaaaat catgcggatg cgttcatcca attccagacg ctgcaacccg
      721 gcggcagcca agataattgc atcgtattcg ccgttgtcga gtttggacaa acgggtttgt
      781 acattgccgc gcaggggttt aatcaccaaa tgcggatagc gcgcgcgcaa ctgggcttcg
      841 cggcgcaggc tggatgtgcc gacaaccgcg ccttcgggca tttcttccaa acgcgcgtat
      901 cggttggaca caaacgcgtc aaacggattg gcgcgttcgc tgatggcggc aagggcgaag
      961 ccttcgggca aatccatcgg cacgtcctta atcgaatgca ccgccaaatc ggcgcgcccg
     1021 tcttgaaggg attgttccaa ctctttgaca aacaagcctt tcccgccgac ttttgacaaa
     1081 gttctgtcca aaatccggtc gccgcgcgtg gtcatgccca aaatttcgac ttcgcaatcg
     1141 ggatacagcg ccttcagacg gccttggata tgtttcgcct gccacatggc aagcaggctt
     1201 tcgcggctgg cgataacaag ttttttcggg ttcatgttca atgcgttcaa agaatggaaa
     1261 cggcgtggag tctatcatat tttgaacgtt tcttcagacg gcaccgtcag gtctatgtta
     1321 tatttacgcc cacaaaacac gaaacccgcc acaatgaaac gcgccaaaaa ataccctttc
     1381 ttaacgttgc agcggcaacg tttccatttg aactttgaaa acgcctcttc cgccgccggc
     1441 atccccgccg aacgcgattt ttaccgctgg gcgtggtctg ccttgaaaaa cgaataccgc
     1501 cgcgccgaca tcggtttgat tcttttggac gaagaagaag cccgcgccta caaccgcgac
     1561 taccggggca aagattatgc caccaatgta ttgagtttcg cgctcaacga aggcgaaatc
     1621 ctgccctgcc aagtttcgga aagactgtac ggcgatttga ttatctgccc gcaagtcgtt
     1681 ttaaaagaag ccgccgaaca gggcaaaacg cctgaacggc attttgccca cttaaccata
     1741 cacggcacgc tgcacctgat gggatacgac cacatcaaag acgatgaggc cgaaataatg
     1801 gaagccgaag aaatccgcct gatgcgggcg gcaggctatc ccaaccccta ccgagaggac
     1861 ggacattaaa atggacggcg cacaaccgaa aacaaatttt tttgaacgcc tgattgcccg
     1921 actcgcccgc gaacccgatt ccgccgaaga cgtattaaac ctgcttcggc aggcgcacga
     1981 acaggaagtt tttgatgccg acacactgac ccggctggaa aaagtattgg actttgccga
     2041 gctggaagtg cgcgatgcga tgattacgcg cagccgcatg aacgtattga aagaaaacga
     2101 cagcatcgaa cgcatcaccg cctacgtcat cgataccgcc cattcgcgct tccccgtcat
     2161 cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc tcaaatatat
     2221 gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt tcgtgcccga
     2281 aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc atatggcaat
     2341 cgtcatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca tcatcgagca
     2401 aatcgtcggt gacatcgaag acgagtttga cgaagacgaa agcgccgaca acatccactc
     2461 cgtttccgcc gaacgctggc gcatccacgc ggctaccgaa atcgaagaca tcaacgcctt
     2521 tttcggtacg gaatacggca gcgaagaagc cgacaccatc ggcggcttgg tcattcagga
     2581 attgggacac ctgcccgtgc gcggcgaaaa agtccttatc ggcggtttgc agttcaccgt
     2641 cgcccgcgcc gacaaccgcc gcctgcacac gctgatggcg acccgcgtga agtaagcaga
     2701 gcctgcccgc accgccgttt ctgcacagtt taggatgacg gtacggtcgt tttctgtttc
     2761 aatccgcccc atccgccaaa cataaaaatg ccgtctgaaa ccggaaaaca ggttcagacg
     2821 gcatttcgct tatccgctta ttttttgctc aaagtcagcc ccgtccagcc gaataacagg
     2881 gttaaagcca aactcaaata gcagaaaaag gcataaggca gatattccca aacgggtacg
     2941 ccaagggcgt ggctgataaa tacgccgcac acgctccacg gcacgagcgg gttaatcacc
     3001 gtccccgcat cttccagagt ccgcgacagg ttgcgcgaat gcaggccgag tttgtcgtaa
     3061 acgggtttga acgtttctcc cgaaagcagg atgctcaaat attgctctcc aatcaggaaa
     3121 ttgaccccga ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag
     3181 gtacggacgg cctccagcag ggaaggaatc acaccgagcg caaacagcag cccgcccaaa
     3241 ctcataccga ggataacgat ggtctgcgta aagaacatac tctccaagcc gccgcgcgaa
     3301 atcagtttgg caatgtcttt aaacgcttcg ccttcgagtt tgtagccgcc ataaaaccac
     3361 gcgccgagct gacgcagatc gggcgtgctg tgcagatacg ttacggcaac ggcggcaatg
     3421 acggtaaaga gcatggcgac cacggcattg acgcgcatca atgccaaaac gaccaacagt
     3481 gcaaacggaa tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag
     3541 gattcgacgc tgttcaaatc ctgcgcggcg acgctgggaa gaagccaaag catcagtgcc
     3601 gcgctgataa gccacgcggg gatggtggtg tacatcatgt ttttgatgtg ttcaaacagg
     3661 tcgataccga cgatggacgc ggaaatgccc gtggtgtcgg aaagcgggga cattttatcg
     3721 ccgaaaaaca caccggaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc
     3781 gccgccatcc ccataaaggc aacgccgaca gtggcgcagg cggtcaggct gctgccgatg
     3841 gacacgccga tgacggaaca cagcgcgaag gcggaaaaat aaaaataagt cggggaaatc
     3901 agcccgaaac cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc
     3961 atcagcccga tgaagaaaaa caggtaaacc gcgcccatac cctgattcaa cgcgcctatc
     4021 atccctgcct gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg
     4081 acgatggcgg caataatgga catatgcggc agccactcca atgaaatgat ggtatagccc
     4141 attgcggcaa tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag
     4201 gatttgaaag caaacatgat tctttgcccg atttgatata aagttaacgg attctaacac
     4261 accatgccga atctgcccga cgttttcaga cggcatttta gcgttttaca aaagccgggc
     4321 agaaacaaaa cggcaatttt ctcgatttaa attaattttt ttgaaatata atttttattt
     4381 ttttatttgt tggcatataa gacttttatg gattgaagta ttaaaacctc ggcacacacg
     4441 ccgcaaacac cggtacgcaa aaatgccgtc tgaaagatgt tcagacggca tttctcaaga
     4501 aaaagattat ttgtccgccc tatatccgta caccgcaaaa aacacgatat acaggtaaca
     4561 gataacggaa acgacaaacg aagacatcag ggtgtaagta tccgccgccc aaccttgaat
     4621 taccggcacg accgcaccgc cgacaatcgc ggtacacagc acaccggaag ccgcgttggt
     4681 gaatttgccc aaccctttgg ttgccaaaga gaaaatcgtc gggaacataa tcgagttgaa
     4741 aaaaccgatg gcaagcagcg accacatcgc cacatccgca ttgccgctgc ccgtcgccat
     4801 cgcgacggca agcagcacga ccgcagccga tgcgttaaac gccaaataac ggttgggcgc
     4861 gaatttcgcc attaccgccg aaccgaggaa acgtccgacc atcgctccgc cccaataaaa
     4921 cgacaggtaa tgcgcggcag aagcatgatc cagccctttc agataaccca acacattgac
     4981 catcagcgaa ccgatagaca cctccgcacc gacgtagcag aaaatgcctg ccgtaccgaa
     5041 cacgagatgt ttgtattgcc atacgccggt tttgccgtcg tggttgtgcg cgctttcctc
     5101 ggcggcaatt ttgcgcgcgt cgggcagccg gatcattttc acgaaaacgg caaggataat
     5161 cagcagcccc gccagtccca aatagggaat ctgtacggaa gaaatctgtt ccgccttgct
     5221 gacggtttgg gttgcgtccg ccaaaatcag gaacgcgccg atttgaggcg caatggtcgt
     5281 acccaaagcg ttaaacgcct gaaccagcgt cagcgttgcc gattccttgc cgggtttcgc
     5341 cagcagggta acataaggat taccggcgac ctgaagcagc gttacgccgg aagccaaaat
     5401 aaacaacgcg cccaaaaata ccgcgtaaga atggctgccc gcagcaggat aaaacagcag
     5461 gcacccgacc gccgtcagca gaaagccgcc gataacgccg tttttgtagc cgactttgcc
     5521 gacaaaagcc cccatcggga tggacattac cgcataggcg gtaaaaaagc aaaattgaat
     5581 cagcatcgcc tgaacgtaag agaggtcgaa aatttctttc aaatgaggga taaggatgtc
     5641 gttcatgcag gtaataaaac ccatcatgaa gaacagcgtg gtcaagacga ccaatgggga
     5701 cgtatggttg ttttgtgatt gcgcagacat gatttgcctt tcgtggtgtc ggaatattgc
     5761 ccgtttcaag aaaatagaaa ttgacatttc ttaacggcaa tgcaaatcat actgatattt
     5821 gaacattttg caaactttat cggatgcagg caatgcgccg atctgaaatt caagcagcag
     5881 aaattaatat tttacagttg tttgcatagg ttttaataga ttttaaaaaa ttccggcggg
     5941 cttgcaaaaa ccgttcccaa taaaatgaaa atcctccgcc gtatccggaa aattcggaaa
     6001 tgccgcatcg gggcatcaaa accggagcgg aaagaatgtc gtatgcccga caaataaagg
     6061 ggcatacggc tgtgccgtat ttttgcaccg tcctccgccc ttcggcgggg gcgtgaaaat
     6121 accgtataat ccgtcgccga tttttacctg tttgggactg tttgcaatga taccctccta
     6181 cacctacgcc gccctcgctt tttccgcctt cacttccgcc accctcctgc ccggcacgtc
     6241 cgaagccgcc ttcgccctgt tcgtccgcaa cttccccaaa cacgcatacg gcgcgttgct
     6301 gtgcgccggc cttgccaacg gattgggcag tatggtttcc tattggatgg gacgtttgct
     6361 gccttcccga aaaatgccgt ctgaaaaaac actgaatctg atacggcgtt tcggtatttg
     6421 gctgcttgcg tttgcgtggc tgcccgtcgt cggcgacgca ctgccgctta ccgccggctg
     6481 gctgcggttg aatccgtgga caagcgggct gatgctggtt atcggcaaaa cggcgcgtta
     6541 cgccttcatt ctgtggggaa tgcaatatta cgccgcctga accgtgcgtt ataatgccga
     6601 ccgcacaaac cgactgcaca aaaaaaatga acagacaaat ccgccaagaa atcttcgagc
     6661 gtttccgcgc cgccaacccc catccgacca ccgagctgaa tttcaactcc cctttcgagc
     6721 ttttaatcgc cgttctgctt tcggcgcagg cgaccgatgt cggcgtaaac aaggcgacgg
     6781 cgaagctgtt tccggttgcc gatacgccgc aggcgatgct ggatttgggc ttggacggcg
     6841 tgatggaata cacgaaaacc atcgggctgt ataaaaccaa gtccaaacac attatgcaaa
     6901 cctgccgcat cctgctggaa aaatacaacg gcgaagtgcc ggaagaccgc gaggctttgg
     6961 aatcgttgcc tggcgtgggg cgcaaaacgg caaacgtggt attgaacaca gcgttcggac
     7021 accccgttat ggcggtcgat acgcatattt tccgcgtgtc caaccgaacc aaaatcgcac
     7081 ccggaaaaga tgtacgcgaa gtcgaagaca aactgatgcg cttcattcct aaagaatttc
     7141 tgatggacgc gcaccactgg ctgattttgc acggacgcta cacctgcaag gctttaaagc
     7201 cgcaatgcca aacctgcatc atcaacgatt tgtgcgaata tcccgccaaa tcctgaccgc
     7261 cgccgccctt caaaatgaaa cgcttgaaaa ttcggacgga cgttgtagaa tttcaaccta
     7321 tgccgtctga agccgacaaa atgcgcttca gacggcatta aagccggtac cggcatccga
     7381 caaaggacaa aacgtgttca aaaaatacca atacttcgct ttggcggcac tgtgtgccgc
     7441 cttgctggca ggctgcgaaa aggcaggcag ctttttcggt gcggacaaaa aagaagcatc
     7501 cttcgtagaa cgcatcgaac acaccaaaga cgacggcagt gtcagtatgc tgctgcccga
     7561 ctttgcccaa ctggttcaaa gcgaaggccc ggcagtcgtc aatattcagg cagcccccgc
     7621 cccgcgcacc caaaacggca gcggcaatgc cgaaaccgat tccgacccgc ttgccgacag
     7681 cgacccgttc tacgaatttt tcaaacgcct cgtcccgaac atgcccgaaa tcccccaaga
     7741 agaagcagat gacggcggat tgaacttcgg ttcgggcttc atcatcagca aaaacggcta
     7801 catcctgacc aatacccacg tcgttgccgg tatgggcagt atcaaagtcc tgctcaacga
     7861 caagcgcgaa tataccgcca aactcatcgg ttcggatgtc caatccgatg tcgcccttct
     7921 gaaaatcgac gcaacggaag agctacccgt cgtcaaaatc ggcaatccca aaaatttgaa
     7981 accgggcgaa tgggtcgctg ccatcggcgc gcccttcggc tttgacaaca gcgtgaccgc
     8041 cggcatcgtg tccgccaaag gcagaagcct gcccaacgaa agctacacac ccttcatcca
     8101 aaccgacgtt gccatcaatc cgggcaattc cggcggcccg ctgttcaact taaaaggaca
     8161 ggtcgtcggc atcaattcgc aaatatacag ccgcagcggc ggattcatgg gcatctcctt
     8221 tgccatcccg attgacgttg ccatgaatgt cgccgaacag ctgaaaaaca ccggcaaagt
     8281 ccaacgcgga caactgggcg tgattattca ggaagtatcc tacggtttgg cacagtcgtt
     8341 cggtctggat aaagccagcg gcgcattgat tgccaaaatc cttcccggca gccccgcaga
     8401 acgtgccggc ctgcaggcgg gcgacatcgt cctcagcctc gacggcggag aaatacgttc
     8461 ttccggcgac cttcccgtca tggtcggcgc cattacgccg ggaaaagaag tcagcctcgg
     8521 cgtatggcgc aaaggcgaag aaatcacaat caaagccaag ctgggcaacg ccgccgagca
     8581 taccggcgca tcatccaaaa cagatgaagc cccctacacc gaacagcaat ccggtacgtt
     8641 ctcggtcgaa tccgcaggca ttacccttca gacacatacc gacagcagcg gcaaacacct
     8701 cgtcgtcgta cgggtttccg acgcggcaga acgcgcaggc ttaaggcgcg gcgacgaaat
     8761 cctcgccgtc gggcaagtcc ccgtcaatga cgaagccggt ttccgcaaag ctatggacaa
     8821 ggcaggcaaa aacgtccccc tgctggtcat gcgccgtggc aacacgctgt tcatcgcatt
     8881 aaacctgcaa taacgcacat aatttaacca aaatattttt aaatgataaa atgcccgtca
     8941 ataccgatta acgggcattt tacaaaaatc agttggttga ttttaaacaa atatttttta
     9001 agacaaaaaa tatttccacc cattttgcca acaaaaccca aaccggaggc ggacagatac
     9061 ccacccatcc ggacgcttaa caccccccat aaaccatgca gctgattgac tattcacatt
     9121 catttttctc ggttgtgcca ccctttttgg cactggcact tgccgtcatt acccgccgcg
     9181 tactgctgtc tttaggcatc ggtattttgg tcggcgttgc ctttttggtc ggcggcaacc
     9241 ccgtcgacgg tctgacacac ctgaaagaca tggtcgtcgg cttggcttgg gcagacggcg
     9301 attggtcgct gggcaaacca aaaatcttgg ttttcctgat acttttgggc attttcactt
     9361 cactgctgac ctactccggc agcaatcagg cgtttgccga ctgggcaaaa cggcacatta
     9421 aaaaccggcg cggcgcgaaa atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg
     9481 actatttcca cagcctcgcc gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag
     9541 tttcccgcgc caaactcgcc tacatcctcg actccactgc ctcgcccatg tgcgtgctga
     9601 tgcccgtttc aagctggggc gcgtcgatta tcgccacgct tgccggattg ctcgttacct
     9661 acaaaattac cgaatacacg ccgatgggga cgtttgtcgc catgagcctg atgaactatt
     9721 acgcgctgtt tgccctgatt atggtattcg tcgtcgcatg gttctccttc gacatcggct
     9781 cgatggcgcg tttcgaacag gctgcgttga acgaagccca cgacgaaacc gccgcttcag
     9841 acgctaccaa aggtcgtgtt tacgcattga ttattcccgt tttggcctta atcgcctcaa
     9901 cggtttccgc catgatctac accggcgcgc aggcaagcga aaccttcagc attttggggg
     9961 catttgaaaa cacggacgta aacacttcgc tggtattcgg cggcacttgc ggcgtgcttg
    10021 ccgtcgtcct ctgcacgttc ggcacgatta aaaccgccga ttatcccaaa gccgtgtggc
    10081 agggtgcgaa atccatgttc ggcgcaatcg ccattttaat ccttgcctgg ctcatcagta
    10141 cggttgtcgg cgaaatgcac acgggcgact acctctccac gctggttgcg ggcaacatcc
    10201 atcccggctt cctgcccgtc atcctcttcc tgctcgccag cgtgatggcg tttgccacag
    10261 gcacaagctg ggggacgttc ggcattatgc tgccgattgc cgccgccatg gcggtcaaag
    10321 tcgaacccgc gctgattatc ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg
    10381 accactgttc gcccatctcc gacacgacca tcctgtcgtc caccggcgcg cgctgcaacc
    10441 acatcgacca cgttacctcg caactgcctt atgccctgac ggttgccgcc gccgccgcat
    10501 cgggctacct cgcattgggt ctgacaaaat ccgcgctgtt gggctttggc acgaccggta
    10561 ttgtattggc ggtgctgatt tttctgttga aagataaaaa acgcgccgac gtttgacctt
    10621 cccccctgtt cagacggcat atgccgtctg aagttttttg gagcaaaccg caatgaccgt
    10681 cccccatatt ccgcgcggcc ccgtcatggc agacattgcc gccttccgcc tgaccgaaga
    10741 ggaaaagcaa cgcctgctcg atcccgcaat cggcggcatc atcctcttcc gccgcaactt
    10801 ccaaaacatc gaacaactca aaacactcac cgccgaaatc aaagccctgc gcacacccga
    10861 actcattatc gccgtcgatc acgaaggcgg cagggtgcaa cggtttatcg aaggttttac
    10921 ccgcctgccc gcaatgaacg ttttagggca aatttgggac aaagacggcg cgtccgccgc
    10981 cgaaaccgct gccggacaag tcggccgggt tttggcaacc gagctttccg cctgcggcat
    11041 cgacttgtcc ttcacgcccg tcttggattt ggactgggga aactgcgccg tcatcggcaa
    11101 ccgcagcttc caccgcaatc ccgaagcggt cgcccgcctc gccctcgccc ttcaaaaagg
    11161 tttggcaaaa ggcggtatga aatcatgcgg caaacatttc cccggacacg ggtttgtcga
    11221 aggggacagc catttggtgc tacccgaaga cgggcgcagc ctcgacgaac tcgaagccgc
    11281 cgacctcgcc cccttccgca ttatgagccg cgaaggtatg gcggcggtca tgcccgccca
    11341 cgtcgtttat ccgcaagtgg acacaaagcc cgcagggttt tccgaaatct ggctgaaaca
    11401 gattttgcgc cgcgatatcg gattcaaagg cgtgattttt tctgacgatt tgaccatgga
    11461 gggcgcgtgc ggagtcggcg gcatcaaaga acgcgcccgc atttcctttg aggcaggttg
    11521 cgacatcata ttggtctgca accgtcccga cttggtcgat gaattgcgcg acggtttcac
    11581 cattcccgac aaccaggatt tggccggacg ttggcaatac atggaaaact cgctcggtca
    11641 tgaggcggta caagccgtta tacagacaac gggtttccaa gcggcgcaag cctttgttgc
    11701 cggccttgcc tcgccgcagg acacggcggg cggcgtaaaa gtcggagagg ccttttaaac
    11761 cggctgcgtt tcaacaatct gccgatttca accgttcgtc agggaaaggc aatgattttt
    11821 tcggtcaaat agaaagttat cttattgaat tactttgaga taaggatgct tatcaatact
    11881 gtattcaaac acagcccttt aaatccgccc gacacgcggc aaaaaggcaa aacggtatag
    11941 gcacgccgta ttttttgcgc tataatcaca ccttaattaa ttcggaatgt agcgcagccc
    12001 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctca ttccgaccat
    12061 attcagaccg cctcgacatc gattgccggg gcggtttttc ccttttggcg gcaacaaaaa
    12121 aatgccgtct gaaacagaat gctgtttcag acggcatttc gttttcccca atcctgccat
    12181 ttgcgcgcac tgacaaaggg tatcggcaag gttcaaaaga ccatcggaat ctaggaagcc
    12241 tggcgtgtac ccaaccgcgg catcttattc attaaggctt cgtcctgatt gggcggcgga
    12301 aatgtcggca gtttcacgcg gaagcggcac actttccccg tatgtcaaac atcggcaaga
    12361 aggtacgcct gccctttggg ttacactgct taaactactc tatccccgcc catttgtgcg
    12421 tctgcacgct caactgccaa tgcacgggcg cgtcggggcg gctgtttaaa ataccgattt
    12481 ggcggatggt gtcgtagatg ttcatcacgc cgttttgttc gcagggagaa agatagtaat
    12541 ggcgcgcacg gattttgcgt tccatatttt cgcaaaacgc aaggacatcg ccgtcggcaa
    12601 caatccgtac ttcgtcggct tcggcgatgc agttggtttc atacttggcg gcgtagcagg
    12661 ctttggggct ggtggcgaca tagtcgattt gcggcggcgc gggcttgagc ccgttggttt
    12721 cgagacagag gaaatagcct tccgccttga gcgtgtccag cagcatatcg agatgcggct
    12781 ggatggtcgg ctctccgccg gtgatgatga tgttgcgggc ggcataagtt ttcagacagc
    12841 ctaagatgtc ggacaggctc atcataccaa atgtcaaata atcggtatca caccagccgc
    12901 acgccagatt gcatttgccc aagcggacga aaacggcagg catacccgtg tttcggccct
    12961 caccttgcag gctttcaaag atttcgacga tgcggtactg gggattttcg ggggcgacgc
    13021 tgattttttt cataccgcca acaccgccgc cgccaaaaat gcgggcaaac cttgtttgac
    13081 gagtatgcct ttgttgccgg acgaaaacgc accccacgcg gcggcaatca ggacgaagcc
    13141 gagaaacagt accgtcgcgc cgtacacggc attatccgga gcggcaaact gcgcccacaa
    13201 aagcccgacg gcaagaaagc cgttgtacag cccttggttg ccaaacaggg tctgcacgcg
    13261 tttctgttcc ataaattcgt aaggcagctt gaacatttcc gccgcttttt cgccgggaat
    13321 ctgcgtcatt tcaagccagg cgatgtagaa atgttcggcg gcgacgagga ggacgagaat
    13381 ggtggagagg agtttcatgg gtgttccttt caatgggtgt gccggtatga aatgccgtct
    13441 gaaaaaacgg ggaatccgtg caggagaaaa aaccgcttta cg
//
LOCUS       DAXDZG010000064        13406 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXDZG010000064 DAXDZG010000000
VERSION     DAXDZG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.064"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            2..1615
                     /locus_tag="ACFB78_001616"
     CDS             2..1615
                     /locus_tag="ACFB78_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1269575.1"
                     /translation="MRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCVW
                     GAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRPQ
                     LLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARYR
                     ALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTEG
                     LTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTAL
                     ADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSGS
                     DGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRAE
                     RNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGWA
                     QEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPTL
                     NYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1676..3550)
                     /locus_tag="ACFB78_001617"
     CDS             complement(1676..3550)
                     /locus_tag="ACFB78_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC1269576.1"
                     /translation="MPYFALFDDAVSGRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB78_001618"
     CDS             3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB78_001618"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFC1269577.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMLRAAYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGAIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB78_001619"
                     /pseudo
     CDS             complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB78_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(6271..7186)
                     /locus_tag="ACFB78_001620"
                     /pseudo
     CDS             complement(6271..7186)
                     /locus_tag="ACFB78_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7359..8159
                     /locus_tag="ACFB78_001621"
     CDS             7359..8159
                     /locus_tag="ACFB78_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1269578.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB78_001622"
     CDS             8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB78_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC1269579.1"
                     /translation="MNFIRSVGAKTLGLIQPFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB78_001623"
     CDS             9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB78_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC1269580.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9559..10149
                     /locus_tag="ACFB78_001624"
     CDS             9559..10149
                     /locus_tag="ACFB78_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC1269581.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGTSLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10200..10478
                     /locus_tag="ACFB78_001625"
     CDS             10200..10478
                     /locus_tag="ACFB78_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC1269582.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10475..11302
                     /locus_tag="ACFB78_001626"
     CDS             10475..11302
                     /locus_tag="ACFB78_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC1269583.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11427..11789
                     /locus_tag="ACFB78_001627"
     CDS             11427..11789
                     /locus_tag="ACFB78_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC1269584.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11789..12172
                     /locus_tag="ACFB78_001628"
     CDS             11789..12172
                     /locus_tag="ACFB78_001628"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC1269585.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            12169..12657
                     /locus_tag="ACFB78_001629"
     CDS             12169..12657
                     /locus_tag="ACFB78_001629"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC1269586.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12891..13019)
                     /locus_tag="ACFB78_001630"
     CDS             complement(12891..13019)
                     /locus_tag="ACFB78_001630"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC1269587.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(13025..13387)
                     /locus_tag="ACFB78_001631"
     CDS             complement(13025..13387)
                     /locus_tag="ACFB78_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC1269588.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
BASE COUNT         2842 a         3771 c         3846 g         2947 t
ORIGIN      
        1 gatgcgccgc gtccgccgcg cgtggggctt gcggcgtttc tgccgccgcc gtccggcggt
       61 gttttttaag gaaggcaaat tgaaggatag tgtgtatcgg gtatgccggc tgtgtgccgc
      121 gctgtgtgtt tggggtgcgg tcggcgcgta tgccgccggc ttgcccgacg ttcgggacga
      181 tgcggcggcg ttgcgcgccc agcgtgcggc tgcggagggt tgggcgggaa tgccgccgga
      241 aggggattct gccgcaaacg gcggttcgcg cgtaatcgac ggggatttcc tgctgtcgcg
      301 cccgcagttg ttggaacacg ttttgcgcga cgcgctcaac ggcaatcagg cggatttgat
      361 cgcttcgttg gcggatttgt atgccaagct gccggattat gatgcggtgt tgtacggcag
      421 ggcgcgggct ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcgctatcg
      481 ggcgttgcac agggataatg cggcagacga gcgggttttg ctggatttgg cggcggcgga
      541 gtttgacgat ttccggctga agtcggcagg gctgcatttt gcggaggcgg caaaattgga
      601 tttgccggcg ccggttttgg aaaatgtggg gcgtttccgg aaaaaaacgg aggggctgac
      661 gggctggcat ttttcgggcg gcatcagccc ttctgtgaac aaaaacgcca acaatgccgc
      721 gccgcaatac tgcctgcagg gcggcggcag ccggatatgc agcgttaccg gacctgtccg
      781 tgcggcaggg ttggattatg aggccggggc ggaaaagctg actgcgctgg cggacaacca
      841 ttatctgctg ttccgcgcca atgtcggggg cacaagctat tatttcagcc gcaaatcggc
      901 ttatgacgat gctttcggca gggcttattt gggctggcag tataaaaatg cgcggcagac
      961 ggtgggggtt ttgccgtttt atcaggcgca attgtcgggt agcgacggct ttgatgcgaa
     1021 aacgaaaccg cctgccgacc gccggcttgc gccgtatatg ctggcgcacg gggcgggtgt
     1081 gcagttggcg cattcttacc gcctgagccg ccggtcgcag ctttcggtgt cgctggaacg
     1141 ttaccgccaa cgctaccgcg aacaaggccg ggcggaacgc aacgacggtt ggcaggacgg
     1201 gctttatgtg tcgctggcaa ggcggttggg cggctcggca accgtgttcg gcggctggca
     1261 gtttgcgcgg tttgtgccga aacgcgaaac ggtgggcggc gcggtcaaca atgccgccta
     1321 ccgccgcaac gccgtccata tcggttgggc gcaggagtgg ggcgggacgg gcggtttgaa
     1381 cagccggatt gccgcgtctt acgcccaccg caactacaaa ggcattgcgg cattttcgac
     1441 ggaggcgcag cgtaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc
     1501 gtacaaaggc atcgtgccga cgttgaacta ccgcttcggt aagacggaaa gcaatgtgcc
     1561 gtatgcaaaa cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt
     1621 gggataatgc cgtccgaact ttgcggcagc aggttcggac ggcatttttg cgcgttcagg
     1681 caaggacggc ggcaaatacg ccgcgcaagg cgttggagag gcggatttct tcggctcctt
     1741 gcagtgtttt ttgtgtgatg tgtgtttcaa tcacttgatt tgtgtgcaaa tatttttgcg
     1801 gttcgtccga cacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg
     1861 tgagccattg tccgcgatgt ttgacgaaca cgttgctcct gccgccttcg agtaggatgc
     1921 cgtccgaatt gaaaaacagg ctgtcgaacg cgccttgcgt ttcggcggtt tgccacgctt
     1981 ggtcgaagat ggcgcggtgt gtggttttga agcggcgcag gtagttttgt gcgggcagga
     2041 tggtcggcga aatgatgacg cgctgtttgt cggtcagacg gtttaaaacg gcgtgggaca
     2101 ggctgatgcc gtctgaagcg agcagggctt tgatgcggaa cgcgccgtcg ggcaagtcgg
     2161 caatgtattg tttgatttga ttttcgcagc cgtcgggcag gggcaggttg agggcttggg
     2221 cggaggtttt cagacggcat aggtggcggt cgagcagggc gcattgcctg ttttccgcgc
     2281 gcagggtttc aaaaatgccg aaatcggggc gcagttcgtt gaggaatcgg gctttccagc
     2341 cgcattcgcg atattcggcg gcggggtcgc tgtcgatgac gatgccggaa ccgacaccgt
     2401 acacgccccg atacggcgga ttggcttcaa acggatgcgg cgtttccccg ccttgtcctg
     2461 ccgttcgggc ttgcgcgttt gaatccgtgc cgccgatgcc tgaaacaatg ccgtctgaaa
     2521 cgggtttgag cgacagggtg cggatgacga cgttgaacgc gccttcaaac cccaagccgc
     2581 cggaacacgg gttcaaatag ccgatgctgc ccgtataaag cccgcgcgct tcggtttcga
     2641 gcgattcgat aatctgcata ctcatttttt tgggcgcgcc ggtgatgctg ccgcagggga
     2701 aggcggcgcg gagggtgtcg gcgaacgagg tgtccggcag ggcttgcgcc cggatggtgc
     2761 tggtcatctg ccagacgctg ccgaaacgcg atactttaaa cggctcgggt acgcatactt
     2821 taccggtttg ggcgattttg ccgaggtcgt tgcgcagcaa atcgacaatc atcacgtttt
     2881 cggcgcggtt tttcgggtct gcttgcaact cggcggcgcg gcgttcgtct tgtccgtcgc
     2941 ccaaaatcgg cgcggtgcct ttcatcggtt cggtgctgat ggtgccgtct gaagcgatgt
     3001 tgaggaagag ttcgggcgag aaacacagcg tccacgcgga ttgcccggct tcatcgggca
     3061 ggtgggacaa tacggcatag gggacgggct ggcgcaggcg gcggtagagg ctgacggggt
     3121 tgccgtaggc ttgcaggtgc aggcgggtgg tgtagttgat ttgataggtg tcgccgcgcc
     3181 ggatggcttc gtggatttgg cggatgcggt cgaggtaatc ggtttcggat acggaggatt
     3241 gcggcgtgga aataccggcg gggatgccgt ctgaatgtcg ggcaagccag ctttcggcat
     3301 cggtgtcggc gcagtcggca aaccagtgca gggcaatgtt gccgccgcgt tcggattcca
     3361 tccccatcag cggcaaaccg aattcgtagt ctgcaaacaa caccgaatgc agcccttttt
     3421 gccatcccga ttgcagcgcg ccgtctaaag catcgagttc ttcgggacgg aaaaaacggc
     3481 tttccacatg attttgatag agttttgcgc ggccgcttac ggcatcgtca aacagggcga
     3541 aataaggcat ggcaattcgg ggcaaatgtt ttgattatac gcccctttta cacggatttt
     3601 cagacggcgg ggcaaattcg ggcaacaaaa tgtaatttta tgtagagaaa gcggtggcga
     3661 aagtgtaaaa ttgtgaaaac ggactttcat ttccaacctg aaatacaata ggagaccttt
     3721 atgacagacc gccagttgca accgtttgaa aacgtagaat tgggcgaaaa gcaagaccag
     3781 ctccaagtat ttgaaaaagc tgttttggaa catgaaggca aaggttctgc cgaagattcc
     3841 ggcacagccc cgctgcccga aaactacccc tgccgcaaac gtatgctccg cgccgcctac
     3901 gaagccgaaa aagccaaact gcaaatcgag ctgctgaaag tgcaaagccg ggtcaaagac
     3961 tccggccagc gcatcgtcag cctgtttgaa ggccgggatg cggcaggtaa gggcggcgcc
     4021 atcaaacgct ttatggaaca tttaaacccg cgtggcgcgc gcgtggttgc tttggagaaa
     4081 ccaaccacta ccgaacgcgg acaatggtat ttccaacgct acatccaaaa cctgccgact
     4141 gcgggcgaaa tggtattctt cgaccgctcg tggtacaacc gcgccggcgt ggaacgcgtg
     4201 acgggcttct gcgaacccaa cgaatatatg ctctttatgt gccaagcccc tgagttggaa
     4261 cgtatgcttg tcgccagcgg catccacctg ttcaaattct ggttctccgt atcccgcgaa
     4321 gaacaactgc gccgcttcat ctcccgccgc gacgacgccc tgaaacactg gaaactttcc
     4381 cccgtggaca tccagtcgct cgaccgctgg gacgactaca ccgaagccaa aaacgcgatg
     4441 ttcttccaca cccacaccgg cgacgcgcct tgggtcatca tccgttcgga cgacaaaaaa
     4501 cgcgcccgtt tgaactgtat ccgctacttc ctgcaccagt tggactaccc gggtaaagac
     4561 gtgaaagcca tcggcaaagt ggacgataaa atcgttcttg ttcccgatac gcgttacaaa
     4621 gagaaaacca tcgatgtcgg tcatgactga ttgccggtga agtaataaaa atgccgtctg
     4681 aacccgattt caggttcaga cggcattttc tatccgtgtg tcaaaccggc atcagatgtc
     4741 ggtttccaga taaatgactt gggtttgcag gtattcttcc aaaccgtgtt tgccgtccgc
     4801 gccgccgatg ccggattttt cccagcccgc gtggaagccc tgcatggctt cgaagttttc
     4861 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgttcag
     4921 gttggtggtg tacacggagc tggtcagacc gaattcgcag tcgtttgcca gggcgatgac
     4981 ctggtcgagc gtgtcgaaag cggaaacggg cagcacgagg ccgaaagttt cttctttcat
     5041 aatgtccata ctgttgtcgg tgtcggtcag cagggtaggc tcgaagaaat agccgcgtcc
     5101 ttcggtgcgt ttgccgccgc aaaccaattt cgcaccttgt ttgacggccc gttccacttt
     5161 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgtg cccatttccg gcgcgcctgc
     5221 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatc gcggcggtca ttttttcaat
     5281 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
     5341 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgct aaatccaaat ccgcgtcttt
     5401 caacacgatg gcgggggctt tgccgccgag ttccagcgag actttggtga tgttggcgga
     5461 agcggcttcc atcacttggc gacccgcttc aaccgagccg gtcaggctga ccatatcgac
     5521 ttgcggatgg gcggacaagg cgttgccgat ttccgcaccc ggaccgtcca ccacgttgaa
     5581 cacgcctgcg ggcagtccga ccgcatcgat gatttcggcg aagatgtggc agttgatcgg
     5641 ggttacgctg ctgggtttga cgacgatggt gttgcccgta accagatccg ggcccatttt
     5701 gcgggcgatc aggaagaagg ggaagttccg cggcaaaatg ccggcaatca cgcccagcgg
     5761 acgtttgaac gataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     5821 acggcgcgcc cattcggctt ggtaatcgag atagtcggca gtgaacatca cttccacacg
     5881 cgccaagtct ttggttttgc cgccttcggc aacgatggtg tcggtcagct cgtcggcgcg
     5941 tccgcgtatg ccttgggcga ttttacgcaa atacgcgccg cgttcgaccg caggcagacg
     6001 ctcccaagcc ggttgcgccg cgcgcgccgc ggtcaacgtc cgccctgccg cctttgggtt
     6061 cgcgggcgat ggtttcttcg gtggacgggt tcaatacgtc gcgccattcg ccgttgaaat
     6121 cgttttcaaa gcgtccgttg atgtacatgg ccaattgttt catttcgggc cctccagttt
     6181 tgtagtcgga tgtagttttg gtttattccc aaataaattg ccgtacaagt ttctttacac
     6241 gcggattttg ggtttcaagt caaatacggc ttaccggttt tcttttttag tttccgcagg
     6301 cgtgctgccg aaatattgcc tgaatgcctg aataaagctg gaaacgtggc gctagccgca
     6361 caaatacgcg gtttcgccca cgtttttgcc cccgttttgc aacagataga gcgcgtgctg
     6421 catttgtttg tggtgcagcc actcgcttgc cgtaatgccg aaatggtcgc gcatacggcg
     6481 ttgcagcgtc ctttcgctga tgttcagcgc ggctgtcagc cggttgactt ggtgtgcgcc
     6541 gccgtcgaac gcggcattca gggtgcggct gaagtcttca gacggcatag cgtctgcttc
     6601 cgccgtttgc cccgccgccg gctcgatgcc gtctgaaacc gtgtcccaca aatccgacag
     6661 cagccgcaac acgtccgcct cgcggcgcaa tgtttcgccc aaatgcccct ttgggacggt
     6721 ttgcaggcag gatgccgcca agccgcgcag gtttgggggc aaatcccata tcctgaccgg
     6781 ttcgcggtaa agcaggggtg cgaaacgcgc gtattccgga cgcggcagcc attgttccat
     6841 acctttaatg gtcattttga ccgttttgcc gccgcgataa agatagcggc tgaacaggac
     6901 ttcttccccg acggcaatta ggacaatctt gccgccgtcc gcatcgatgc ggaagcggcg
     6961 gcggttgatg ccgaagtcca aacgcccttc cagcaacagc acgaacgaca cataaggttc
     7021 ggcgaggcgg ctgctgcaaa aatcgcaccg tgccgttacc gtaccgccgt ggatggaaat
     7081 gccgttggac aaagtatcga aacggtagct gccctccaca taagccgccg ccgcttttga
     7141 ccagattgac gaaccggctg ctgttcaaat gttctgcttt ttccatagtt ttctgataaa
     7201 ggttatcatt tgaaagataa catttttcgc cacagcaaac aatccgccgc cgacaaaggc
     7261 attttcacaa cacttgccgt ccgtatgccg tctgaaaatt gcccgtcttc ccgataagcc
     7321 ttaaaataca cgccgtcaaa cctaccgtcc gcccgtacat gagcccatcc ccctttatcg
     7381 aaatgaaaga cgtcgccttc gcgtatggcg accgcccgat tctgaacgac atcaatttca
     7441 gcattccgca aggcaatttt gccgccgtga tgggcggttc gggcagcggc aaaaccacgc
     7501 tgatgaggct gattacagga cagattcgtc cgcagtccgg gcaggttttg attgaaggac
     7561 gggatttggc gggtttttcg gctgacgaac tctacgaaca ccgccgccgt atgggcgtat
     7621 tgttccaaca cggtgcgctg tttaccgatt tgtcggtatt cgacaatatc gcttttccga
     7681 tgcgcgaact gacgcaactt ccggaagcgg ttattcgcga tttggttttg ttgaaattga
     7741 acgcggtcgg tctgcgcggc gtggaaaacc tgatgccgtc cgagttgtcc ggcgggatgt
     7801 cgcgccgcgt cgcgctggca cgcacaatcg cgctcgaccc cgaaattatg ttgtacgacg
     7861 aaccgtttac cggcctcgac ccgatttcct tgggcgtgat tgcccacttg atcagccgcg
     7921 tcaacaaggc cttgcgttcg accagcatta tggtaacgca cgacattgaa aaatctttgg
     7981 aaatcgtcga tcaggtaatt ttcttggcgc acggcgaaat tatgttctcc ggctcgccgc
     8041 aggaaatgcg cgaactggat tcgccttggg tgcgccagtt tgtcggcggg ctggcagacg
     8101 gccccgtggc ataccgctat ccggcgcaaa cgtcgttgca gcaggatttg ctcgggtaaa
     8161 tgagcaggtg ccgtctgaaa ccgttgaacc ttacatatag aaatttatga attttatccg
     8221 ttccgtcggg gcgaaaaccc tcggccttat tcaacccttc ggcagtatca cgctgtttct
     8281 gctgaacatt ttggcgaaat ccggcacggc tttcgcccgt ccgcgcctga gcgtgcgcca
     8341 agtgtatttt gccggcgtgc tgtcggtgct gattgttgcc gtttcggggc tgttcgtcgg
     8401 tatggttttg ggtttgcagg gctatacgca gttgtcgaaa ttcaaatccg ccgatatttt
     8461 gggctatatg gtcgcggctt ctctgttgcg cgaactgggt cccgtgttgg cggcgattct
     8521 gtttgccagc agcgcgggcg gtgcgatgac cagcgaaatc ggtttgatga aaacgaccgg
     8581 acagctcgaa gcgatgaacg tgatggcggt caaccccgtc gcccgcgtgg ttgccccgcg
     8641 tttttgggcg ggcgtgtttt ctatgccgct tttggcttcg attttcaacg tcgcgggcat
     8701 tttcggcgcg tatttggtcg gcgtgagctg gctgggtttg gacagcggta ttttctggcc
     8761 gcagatgcag aacaacatta cgatacatta cgatgtaatc aacggtttga tcaaatccgc
     8821 cgcgttcggc gtggcggtaa cgctgattgc cgtgcatcag ggcttccact gcatcccgac
     8881 ttcggaaggc attttgcgcg ccagcacgcg cacggtggtt tcgtccgccc tgacgatttt
     8941 ggcggtcgat tttatattga ccgcgtggat gtttacagat tgacagactg tctgaagacg
     9001 aaacgcggaa catcggatat tcaaggaact ttaatgaaaa agaacatatt ggaattttgg
     9061 gtcggactgt tcgtcctgat cggcgcggcg gcggttgcct ttctcgcttt ccgcgtggcg
     9121 ggcggcgcgg cgttcggcgg ttcggacaaa acttacgccg tttatgccga tttcggcgac
     9181 atcggcggtt tgaaggtcaa tgcccccgtc aaatccgcag gcgtattggt cgggcgcgtc
     9241 ggcgctatcg ggcttgaccc gaaatcctat caggcgaggg tgcgccttga tttggacggc
     9301 aagtatcagt tcagcagcga cgtttccgcg caaatcctga cttcgggact tttgggcgaa
     9361 cagtacatcg ggctgcagca gggcggcgat acggaaaacc ttgctgccgg cgacaccatc
     9421 tccgtaacca gttctgcaat ggttctggaa aacctgatcg gtaaattcat gaccagcttc
     9481 gccgagaaaa acgctgaggg cggcaatgcg gaaaaagccg cagaataaac atacacatcc
     9541 attttcagga aagacatcat gaaaaaatcc tccttcatca gcgcattggg catcggtatt
     9601 ttgagcatcg gcatggcatt tgcctccccg gccgacgcag tgggacaaat ccgccaaaac
     9661 gccacacagg ttttgaccat cctcaaaagc ggcgacgcgg cttctgcacg cccaaaagcc
     9721 gaagcctatg cggttcccta tttcgatttc caacgtatga ccgcattggc ggtcggcaac
     9781 ccttggcgta ccgcgtccga cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg
     9841 ctgatccgca cctattccgg cacgatgctg aaattcaaaa acgcgaccgt caacgtcaaa
     9901 gacaatccca tcgtcaataa gggcggcaag gaaatcgtcg tccgtgccga agtcggcatc
     9961 cccggtcaga agcccgtcaa tatggacttt accacctacc aaagcggcgg caaataccgt
    10021 acctacaacg tcgccatcga aggcacgagc ctggttaccg tgtaccgcaa ccaattcggc
    10081 gaaatcatca aagccaaagg catcgacggg ctgattgccg agttgaaagc caaaaacggc
    10141 ggcaaataac cgacaaacca taccgtccga gggcggtatg ggtttttcga acaaacgcta
    10201 tgcatacaga actcaaaaac ggaacactgc acatcggcgg cgacatcacc gtcaaaaccc
    10261 tgaccgggga cgcatttggg cgtttccggc aacaatgccg tctgaaagaa accattgccg
    10321 tcgatttcgg aggtgtcaaa cgtgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc
    10381 tgcgcggctg caaaggcagc gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt
    10441 ccgaactgta cgaaatcaaa gactggctga aatcatgaaa aaaaccgcct acgccatcct
    10501 cctgctgatc gggttcgctt ccgcccctgc atttgcagaa acccgccccg ccgaccctta
    10561 tgaaggctac aaccgcgccg tttccaaatt caacgaccaa gccgaccgct acattttcgc
    10621 ccctgccgcg cgcggctacc gcaaagttac gccgaaaccc gtccgcgccg gcgtgtccaa
    10681 tttttttaac aacctgcgcg acgtggtcag tttcggcagc aatatcttgc gtttggacat
    10741 caaacgcgca agcgaagacc tcgtccgcgt cggcatcaat accaccttcg gtttgggcgg
    10801 gctcattgat attgccggcg cgggcggcgt tcccgacaat aaaaacactt tgggcgacac
    10861 gtttgcctcg tggggctgga aaaacagcaa ttatttcgtg ttgcccgtct taggcccgtc
    10921 caccgtccgc gacgcgctcg gcacgggcat tacctctgtt tatccgccca agaatatcgt
    10981 tttccatacc cctgccggac gctggggcac gactgccgct gccgccgtca gtacgcgcga
    11041 aggcctgctc gatttgaccg acagtctgga cgaagccgcc atcgacaaat acagctacac
    11101 gcgcgacctc tatatgaaag tccgcgcacg gcagaccggt gcaacacctg ccgaaggtac
    11161 ggaagataac atcgacatcg acgaattggt cgaaagtgcc gaaaccggcg cggcagagcc
    11221 cgccgttcac gaagattccg tatccgaaac acaggcagaa gcagcagggg aagccgaaac
    11281 gcaacctgga acacaaccct aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc
    11341 ccttccgtcg gaacggacag tccgacacta cagattgggc tataatccct tttttgcgca
    11401 taccgcgcga cagcaaagga aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca
    11461 tccctttaga acctttttgg aactggctgc aaaccctgcc gggcaaccac ctcgacggac
    11521 tgaccctcga agacatccaa gccgatgcca attcctacct tgtccgcccg tgcgaaaccg
    11581 ccgacgaagt atgggacgaa atcgaagccc gtttcgaaga cattttcgcc gccgaacttg
    11641 ccgactggtg cgaagacgaa cgcgagtggc ccgcactcga tgccgacatt ttcaacgaat
    11701 ggttcgacat ccagctttct accgtcatca ccgacctcga acacgaaccg cttgcccgcg
    11761 aagccttcca acccatcaac ctgaactgat gaagctgacc gtccgcaatt accatctcga
    11821 cggctacggt catgtcaaca atgcacgcta cctcgaattt ctcgaggaag cgcgctgggc
    11881 gttttttgaa aaacgcggac tgatgcacga gttggcaggc ctcatactga ttgtcgcccg
    11941 catcgacatc cgatacagcc gtcccgccgt cgaaggcgac gtattgcagt tttcatgccg
    12001 cctgaaaacc cccggcatgc gccgcattgt gctgacccag acgattacac tgccaaacgg
    12061 caaaaccgcc gcagaagccg acatcaccct gatgcccgtc cacgccgcca cacaacgcac
    12121 cgtcagcctg cccgccaccc ttgcccgcgc actggaagcc ttgtccgaat gaaaaaaata
    12181 ctcaccgccg ccgccgtcgc actgatcggc atcctccttg ccaccgtcct catccccgac
    12241 agtaaaaccg cgcccgcctt ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc
    12301 gacctgcaag gcaaagtcac cctgattaat ttttggtttc cctcctgtcc gggttgtgtg
    12361 agcgaaatgc ccaaagtcat caaaacggca aacgactaca aaaataaaga tttccaagtc
    12421 ctcgccgttg cccagcccat cgatccgata gaaagcgtcc gccaatacgt caaagactac
    12481 ggactgccgt ttaccgtcat ttatgatgcg gacaaagccg tcggacaggc attcggcaca
    12541 caggtttatc cgacttccgt ccttatcggc aaaaaaggcg aaattctcaa aacctacgtc
    12601 ggagaacccg atttcggcaa actctaccaa gaaatcgata ccgcgctggc acaatagcga
    12661 tgccgtctga agcgcaaaaa aagcaggaac gatgttcctg ttttttataa tggattaaca
    12721 aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa cgattcccta aggcgcccaa
    12781 gcaccaagcg aaccggttcc gtaccatttg tactgcctgc ggcccgccgc cttgtcctga
    12841 tttttgttaa tccactatag attatccgcc ccgacacgtt caggcggcat tcaaccttcc
    12901 ggcagattct ttttcaccac cgcaggattg cctgccgcca aagagtaggg cggaatgtct
    12961 tttgtaatca ccgcgcctgc gcccacgacc gagccgcgtc cgacggttac gcccgccatc
    13021 acaatcaccc tgcgccccgg ccagacatcg tcctccaacg taatcggacg gatttccgtg
    13081 tagccctcaa agcgtttttt tcacggtcaa acttgtggtt ggtcgaatag agcaggcatt
    13141 ccggccccat catcacattt ttgccgacca ccagcccacg gcagatttca cagtttgccc
    13201 cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac atacgccccg cgtccgatat
    13261 gcggcgaaat ccgccgcgcc agaaaaccga gcacccgtct gccgatgatg ccgatgccgc
    13321 gcatatgcga cggcggcaac accgaaccgg caaaacgcag cagcaggaaa aagaattttg
    13381 atcccatcaa acctccctca aaagaa
//
LOCUS       DAXDZG010000065        12771 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXDZG010000065 DAXDZG010000000
VERSION     DAXDZG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.065"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..365
                     /locus_tag="ACFB78_001632"
     CDS             <1..365
                     /locus_tag="ACFB78_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC1269589.1"
                     /translation="GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMG
                     APVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSG
                     KYAFARRVKKNDPSRFLN"
     gene            384..602
                     /locus_tag="ACFB78_001633"
     CDS             384..602
                     /locus_tag="ACFB78_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269590.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMAENMSELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            747..1091
                     /locus_tag="ACFB78_001634"
     CDS             747..1091
                     /locus_tag="ACFB78_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC1269591.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            1091..1783
                     /locus_tag="ACFB78_001635"
     CDS             1091..1783
                     /locus_tag="ACFB78_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC1269592.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGMGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            1850..3070
                     /gene="argJ"
                     /locus_tag="ACFB78_001636"
     CDS             1850..3070
                     /gene="argJ"
                     /locus_tag="ACFB78_001636"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC1269593.1"
                     /translation="MAVNLTEKTTEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            3130..4539
                     /locus_tag="ACFB78_001637"
     CDS             3130..4539
                     /locus_tag="ACFB78_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC1269594.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            4727..6118
                     /gene="hrpA"
                     /locus_tag="ACFB78_001638"
     CDS             4727..6118
                     /gene="hrpA"
                     /locus_tag="ACFB78_001638"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC1269595.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            6186..7687
                     /locus_tag="ACFB78_001639"
                     /pseudo
     CDS             6186..7687
                     /locus_tag="ACFB78_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            7914..9491
                     /gene="lpt3"
                     /locus_tag="ACFB78_001640"
     CDS             7914..9491
                     /gene="lpt3"
                     /locus_tag="ACFB78_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC1269596.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            9557..>12771
                     /locus_tag="ACFB78_001641"
     CDS             9557..>12771
                     /locus_tag="ACFB78_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC1269597.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     SFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         3299 a         3732 c         3041 g         2699 t
ORIGIN      
        1 tgggtattgc ctaccgctac ggcggcacat cggtttctac cggttttgac tgcagcggat
       61 tcatgcagca catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg tcggcggaac
      121 aggcgcggat gggcgcaccc gttgcccgaa gcgaattgca gcccggggat atggtgtttt
      181 tccgcacgct cggcggcagc cgcatttccc atgtcggact ttatatcggc aacaaccgct
      241 tcatccacgc gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc cacaaatatt
      301 ggagcggcaa atatgcgttc gcccgccggg tcaagaaaaa cgacccgtca cgctttctga
      361 actgatttcc caaggaatac gcaatgagta tgcccgaaat gcccaaatgg tacggcgatg
      421 acggacagat cgtatcctgt accgaaaagg tcaaagtaat ggccgaaaac atgtctgaac
      481 tgtaccaaac agcacaggat gcatttgaag acgcgctgct gatgggttgc ggcgaacggc
      541 agttgcgcgc ttacctgctc gcgctgattg aaggtttgga aaatccctac cgcaaagtct
      601 gaacacgccc cggttgctgc ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc
      661 ttcggctttt cagacggcat atttgacgtt atgattaaac agttaacaag atttatcaca
      721 acgccgtcaa aagacagaca cacaacatga acatcatccg cgcgctcctc atcatcctcg
      781 gctgcctcgc cgccggcgaa accgccgttt tcctagcagg catcaaactg cccggcagca
      841 tcgtcggcat gggcgtgctg tttgcgcttt tgcaggcggg ttgggtcaaa acgtcttggc
      901 tgcaacagct taccgacgcg ctgatggcaa acctgacgct gttcctcgtg ccgccctgcg
      961 tggcggtcat cagctatttg gatttgattg ccgacgattg gttttcgata ctggtttccg
     1021 cctccgccag cactttgtgc gtactgctgg ttacgggcaa ggttcaccgc tggatacgga
     1081 gcattatctg atgagcgaaa tcctcaggca gcccggcatc ctgcttttcc tcacgcttgc
     1141 cgtgtacgcg cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt
     1201 actcgtcagc actatcgtgc tgattgccta cctgaaaatc ctcggtatcg attatgcggt
     1261 gtaccacaac gccgcgcaat tcattgattt tcggctgaaa cccgccgtcg tcgtgcttgc
     1321 cgtgccgctc taccaaaacc gccgtaaaat cttcaaccag tggctgcccg tcatcgtttc
     1381 gcagcttgcg ggcagcgtta cgggcattgt tacggggatg tattttgcca agtggctggg
     1441 cgcggaacgc gaagtcgtcc tcccgctcgc gtccaaatct gttaccaacc ccatcgccat
     1501 cgaaatcacc cgctccatcg gcggcattcc cgccattacc gccgccaccg tcatcattgc
     1561 cggtctggtc ggacagattg ccggttacaa aatgttgaag aacacggtcg tcatgccctc
     1621 gtccgtgggt atgtcgctcg gcacggcttc gcacgcgatg ggcattgccg cctcgctcga
     1681 acgcagccgc cgcatggcgg catacgcggg gatggggctg acgttcaacg gcgtgctgac
     1741 cgcgctgatt gcgccgctgc ttatccccgt tttggggttc tgaacccgtt tcagacggca
     1801 tttcaaccca tgccgtctga acgccgacac actcgcaagg agaaccgtta tggctgtcaa
     1861 cctgaccgaa aaaaccaccg aacaactgcc cgacatcgac ggcattgccc tctacaccgc
     1921 ccaagcaggc gtgaagaagc ccgggcatac cgacctgaca ctgattgccg tagccgccgg
     1981 cagcaccgtc ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc
     2041 caaatcgcac cttttcgacg aagacggcgt gcgcgccctc gtcatcaaca cgggcaacgc
     2101 caacgcgggt acgggcgcac agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc
     2161 ccggcaaatc ggctgcaaac cgaaccaggt gctgcccttc tccaccggcg tgattctcga
     2221 accgctgccc gcagacaaaa tcatcgccgc cctgcccaaa atgcagcctg ccttctggaa
     2281 cgaagcggca cgcgccatca tgaccaccga caccgttccc aaagccgcct cgcgcgaagg
     2341 caaggtcggc gaccaacaca ccgtccgcgc cacaggcatt gccaaaggct cgggcatgat
     2401 tcatcccaat atggcgacca tgctcggttt catcgccacc gatgccaaag tttcccaacc
     2461 cgtcctccaa ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga
     2521 cggcgacacc agcaccaacg acagcttcgt catcatcgcc accggcaaaa acagccaaag
     2581 cgaaatcgac aacatcgccg acccgcgtta cgcccaactc aaagaattgt tgtgcagcct
     2641 tgcgctcgaa ctcgcccaag ccatcgtccg cgacggcgag ggcgcgacca agttcatcac
     2701 cgtccgcgtc gaaaacgcca aaacctgcga cgaagcccgc caagccgcct acgccgcggc
     2761 acgttcgccg ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg gcaggctgct
     2821 cgccgccatc ggttatgccg acgttgccga cctcgatacc gacctcgtgg aaatgtatct
     2881 cgacgatatt ttggttgccg aacacggcgg acgcgccgca agctacaccg aagcacaagg
     2941 gcaggcggtg atgtcgaagg acgaaatcac cgtccgcatc aagctgcatc gcggacaagc
     3001 cgccgccacc gtctatacct gcgacctgtc gcacggatac gtttccatca acgccgacta
     3061 ccgttcctga cccgacacgg cttcagacgg catacataaa atgccgtctg aaccgtcgga
     3121 caacatacca tgacctccac attcccccgc cgcctcgccc gcaaaatccg ccaaacccgc
     3181 cgcctgtcgc gcaaaagcat cgcctttctg ttccttctgg caggttcggc actcgtcgcc
     3241 ctgaccgcac tgttttttgc ccatcttgcc gattttgcgc tggaactgaa cgccaaactg
     3301 gttcaacaat acccgtggtt cgcatggatt gccctgccgc cgggactgcc cgtcatcgtc
     3361 tggctcacac gcaaattcgc ccccttcacc gccggcagcg gcatccctca agtcatcgcc
     3421 tcactgtcgc tgccctacgg cgcacaaaaa acgcggctga tacgcctcgg gcaaacgctg
     3481 ctgaagattc cgctgacctt tttgggtatg ctgttcggcg cgtccatcgg acgcgaaggc
     3541 ccgtccgtgc aggtcggcgc ggcagtaatg ggcgcgtggg gcgcgtggtg caaaaaacac
     3601 ggtttggcgt ttaagggtat gcaggaaaac gatttgatgg cggcgggcgc ggcgggcggt
     3661 ttggcagcag cgttcaacgc gccgctggcg ggcgtgattt tcgccattga ggaactgggg
     3721 cgcggcatca tgctgcgctg ggagcggcag attctgctcg gcgtactcgc cgccggtttc
     3781 atacaggtcg ccattcaggg caacaacccg tatttttcgg gtttcaacgg cggcgttttg
     3841 gaaaatattt tgatgtggat cgtggcggcg gggctggttt gcggcgcggc tggcggcata
     3901 tttgcccgta tgctccatcg cggtgcggcg gcgtttgcac cgcgcaagat acgcggcttc
     3961 atccgcaacc gtccgctgct gctggcggca ctgatggggc tgctgctcgc cctgctcggc
     4021 acgttctacc aaggcaaaac ctacggcacc ggctaccacg aagccgccca agccctgcac
     4081 ggcatctacg aagcccccgt cggactcgcc gccgccaaat ggctcgccac cgtattcagc
     4141 tattgggcag gcatcccggg cggcattttc actccctcgc tgaccatagg tgcggttttg
     4201 ggcgagcata tcgccgccat cgccgacata tcgcagggtg caaacatcat cgtcctcatc
     4261 tgcatggcgg catttttagc gggcgcgaca caatcgccga ttacttccgc cgtcgtcgtc
     4321 atggaaatga ccggcgggca aagcctgctg ttttggatgc tgattgcctg cattttcgcc
     4381 tcgcaggttt cgcgccagtt ttcgccgcgt ccgttctacc acgcatcggg aatgcgcttc
     4441 cgccggcgcg tgcttcagga aaccgccgcc caaaccggca atgcgcccgc aagaccgcaa
     4501 gccgcaaaca gcaaaacggg aatgccgtct gaaaattaaa acgcccccga tcaaacgccg
     4561 gcagccgcct tgattcaaat accgttccgc tgcgcttgaa atttcagcaa caatgccgtc
     4621 tgaacgacag aatgcggttt tcagacggca tttccccatc ccgatattgt tcaaacaaaa
     4681 ccgaagcgtt tgctataatt gcacttttta ccgcacacgc acgcccatgt ttcccgattt
     4741 ctcccaaacc ctctccaaag accgccactt cctgcaatcc gccttcaaaa atcccaacaa
     4801 atacggcggc ttgtccaaaa tcgaagaaaa ataccgaaaa tcgcacgaaa tcttcttgaa
     4861 acgtttggcc gcattgccaa aacccgaatt cgacaacacc ctgcccgttc acgaaaaact
     4921 cgaagaaatc aaaaaaaccg ttgccgaaaa tcaggtaacg attatctgcg gcgaaaccgg
     4981 ttcgggcaaa accacgcagt tgcccaagat ttgtttggaa ctcgggcgtg gggcggcagg
     5041 attgatcggg catacccagc cgcgccgttt ggccgcgcgt tcggtggcgg agcggattgc
     5101 cgaagagctg aaatccgaaa tcggcagcgc ggtcggctat aaagtgcgct ttaccgacca
     5161 cacctcgcgc gatgcctgcg tcaagctgat gaccgacggc atcctgctgg cggaaaccca
     5221 gaccgaccgt tatctcgccg cctacgacac gattatcatt gacgaagcgc atgaacgcag
     5281 cctgaacatc gacttcctct tgggctacct aaaacaactc ctgccgcgcc gccccgattt
     5341 gaaagtcatc atcacatcag ccaccataga cgcagaacgc ttctcccgac acttcaacgg
     5401 cgcgcccgta ctggaagtga gcgggcgcac ctatcccgtc gaaatcctct accgaccgct
     5461 gaccggcaaa gacgaagacg acgcagaagt cgagctgacc gacgcgattg tcgatgccgc
     5521 cgacgaattg gcacgctacg gcgaaggcga tattttagta ttcctgccgg gcgaacgcga
     5581 aatccgcgaa gcggcagaag ccctgcgcaa atccacactg cgccgcaatg acgaaatcct
     5641 gcccctgttt gcacgcctgt cgcacgccga acagcacaaa atcttccacc cttcaggcgc
     5701 aaaatgccgc atcgtgctgg cgaccaacgt cgccgaaacc tcgctcaccg tgccgggcat
     5761 caaatacgtc atcgacaccg gcctcgcgcg cgtcaaacgc tattccgcgc gggcaaaagt
     5821 ggaacaactt cacgtagaaa aaatctccca agccgccgcc cgccaacgct ccggccgctg
     5881 cggacgcgtc tcagcaggcg tgtgtatccg actgttttca gaagaagatt ttaacagccg
     5941 tcccgaattt accgatcccg aaatcgtccg cagcaacctc gccgccgtca tcctgcgcat
     6001 ggcatcgctg aacttgggcg acgtagcagc attcccgttt ttagaaatgc ccgactcgcg
     6061 gtatatcaat gacgggtttc aggtgttgtt ggaattgggg gcagtggagg ttgtctgaaa
     6121 acaggcagat cgggcgttta tgcccaacga gaaccttatt gtcactatta ttagggatta
     6181 atacaatgaa tataaaagaa tttatgtcta actataccaa ccatcccgtt ctctttattg
     6241 gaacaggtat gagtttgaga tacttagata attcatatac ttgggatggt ttattatcta
     6301 aaattgcaat agatttattt ggagatgata gggaatattt gaacatcaaa tcacggtact
     6361 gtgaagatgg tagattccaa tatgaagaga ttgcagagga attacaaagt aaatttgata
     6421 aagttttaga aaatgaccct gatggtagat ttaaagaaat aaatgataag ttctttgaaa
     6481 atatgagggc gggaaacacc ttgagcaggt ttaagattta tatatccacc ctgctctcac
     6541 aattgaatta taaagataat tctaatacag aattatctga attaaaaaaa gcgagaaaaa
     6601 atgtagggtc aatcattaca acaaattatg ataaattagc ccaagatatt tttgaattta
     6661 acccactaat tggtaatgat attcttttaa gcaaccctta tggctcagta tacaaaatac
     6721 atggttgtgt ggacgatcca tcaaaaatta ttattaccaa aaaggattat gagaaattta
     6781 aagaaaaata tgaacttatt agagcccaac tattatcgtt atttattcac aatccaatca
     6841 tatttcttgg atataatgtt ggtgacgaaa atatcaaaga aattttaaaa acaatcttta
     6901 cttatgtaga accaaactct ccttcagcta ataaaattcg taggaatttt ttactcgtag
     6961 aatatgaacc tgagtctaac aatgaagata ttgttgaaca tgatatagat ataactggat
     7021 tctctactat ccgtattaat aaaatcaaaa cagataactt ctcacaaatt tataaagctc
     7081 ttgcagaact aacactacca atctcagcta tggatgtacg taaatttcaa tctatagcaa
     7141 aggagattta tactggcggt aacattaaag ttagctttac ggaagatatg gacaatttaa
     7201 ataatagcga taaagtggtt gctattggtt caactaaaac tatcagctac aactttcaaa
     7261 caacatcaga gatgatgtca aattatttca aaataattga agaggaaaat tcacaacttc
     7321 taaaattaat agataaacat agtatagcat ctacgcaata ttttcctatt tatggattta
     7381 gtaggatatg ttctgatata cataaagaag ctgtactaaa acgccaacaa aaagaaaaat
     7441 tagatcattt tattgaagaa ataaataggc gttgtaaaaa taatcattca tccatccaat
     7501 caattttaga tgatgaaaat atttcagaca catataaaaa cgatgcgatt gcttggggaa
     7561 tatggaataa ccaactttca gaagatgagg ttgaaaatta tttaaaaaat tttgtgaata
     7621 aaaaaatacg cactataaaa gactactatg tatgttcgat tataaaaaat atgcggatac
     7681 tgtctaaact ctaaaaaata aggtcgggca tttatgtccg acctacaaaa ctatgcaaaa
     7741 cactaaaaat caagcacgca gctcaggcat tcatgcccaa cacaatccga caaatgacaa
     7801 accactttca gacaacctcg ttttttgaaa aacaatctgt aaacagatag ctgctgaaga
     7861 ataccgttgc cgagccccaa aacccgtcct gcaactttta ttgtgaactg cccattatga
     7921 aaaaatccct tttcgttctc tttctgtatt catccctact taccgccagc gaaatcgcct
     7981 atcgctttgt attcggaatt gaaaccctac cggctgcaaa aatggcggaa acgtttgcac
     8041 tgacatttgt gattgctgcg ctgtatctgt ttgcgcgtta taaggcaacg cgtttgttga
     8101 ttgcggtgtt tttcgcgttc agcattattg ccaacaatgt gcattacgcg gtttatcaaa
     8161 gctggatgac gggtattaac tattggctga tgctgaaaga ggttaccgaa gtcggcagcg
     8221 cgggcgcgtc gatgttggat aagttgtggc tgcctgcttt gtggggcgtg gcggaagtcg
     8281 tgctgttttg cagccttgcc aagttccgcc gtaagacgca tttttctgcc gatatcctgt
     8341 ttgccttcct aatgctgatg attttcgtgc gttcgttcga cacgaaacaa gagcacggta
     8401 tttcgcccaa accgacatac agccgcatca aagccaatta tttcagcttc ggttattttg
     8461 tcggacgcgt gttgccgtat cagttgtttg atttaagcaa gatccctgtg ttcaaacagc
     8521 ctgctccaag caaaatcggg caaggcagta ttcaaaatat cgtcctgatt atgggcgaaa
     8581 gcgaaagcgc ggcgcatttg aaattgtttg gttacgggcg cgaaacttcg ccgtttttaa
     8641 cccggctgtc gcaagccgat tttaagccga ttgtgaaaca aagttattcc gcaggcttta
     8701 tgacggcagt atccctgccc agtttcttta acgtcatacc gcacgccaac ggcttggaac
     8761 aaatcagcgg cggcgatacc aatatgttcc gcctcgccaa agagcagggc tatgaaacgt
     8821 atttttacag tgcccaggct gaaaaccaaa tggcaatttt gaacttaatc ggtaagaaat
     8881 ggatagacca tctgattcag ccgacgcaac ttggctacgg caacggcgac aatatgcccg
     8941 atgagaagct gctgccgttg ttcgacaaaa tcaatttgca gcagggcagg cattttatcg
     9001 tgttgcacca acgcggttcg cacgccccat acggcgcatt gttgcagcct caagataaag
     9061 tattcggcga agccgatatt gcggataagt acgacaacac catccacaaa accgaccaaa
     9121 tgattcaaac cgtattcgag cagctgcaaa agcagcctga cggcaactgg ctgtttgcct
     9181 atacctccga tcatggccag tatgtgcgcc aagatatcta caatcaaggc acggtgcagc
     9241 ccgacagcta tattgtgcct ctggttttgt acagcccgga taaggccgtg caacaggctg
     9301 ccaaccaggc ttttgcgcct tgcgagattg ccttccatca gcagctttca acgttcctga
     9361 ttcacacgtt gggctacgat atgccggttt caggttgtcg cgaaggctcg gtaacaggca
     9421 acctgattac gggcgatgca ggcagcttga acattcgcaa cggcaaggcg gaatatgttt
     9481 atccgcaata agcagcgtga aaaccaataa agacaaattt agatgatgtc ggggaagatg
     9541 cccgaccgac aagactatgc aaaatatgaa aaaccaagta cgcggatcag gcatggatgc
     9601 ccgatccaac ccggccaatg tttcagacgg cctgcaaaac agttcgggcc atatcggtac
     9661 caacacgcgc taccgcctga ccaagctcgg cgaacaaatg gcgcgcctgc ccatcgatcc
     9721 gaaaattgcg cgcattttgc tggcggcgaa gaaacacgac tgcatggcgg aaatattggt
     9781 gattgcgtct gcgctgtcga ttcaagaccc gcgcgaacgg ccgctggaag cgcgcgatgc
     9841 cgcggccaag gcgcatgaac gctttaccga caagcaatcc gatttcctta cctacctgaa
     9901 tatttgggac agtttccagc gcgagcgcga caaaggtttg tccaacaagc agttggtgca
     9961 atggtgccgt caatattttc tgtcgcacct gaggatgcgc gagtggcgcg agctgcacca
    10021 ccagcttgcc caaaccgcga ttgaaatggg cttgaccacc aaagaagctg cgtttagacg
    10081 acctcccgaa gtcaagcaac tgacgtcgtc tgagaatgcg ggcgaccaag acctatctgc
    10141 caaactcaaa caaaaacaac tggataagaa acaacaccgc acccaaatcc gcgctaccaa
    10201 agaagcgggc tacgaacaaa tccaccgcgc cctgctcacc ggcctcatcg ccaacgtcgg
    10261 catgaaatcg cccgacagca acgactacac cggcgcgcgc ggcagccgct tccacctttt
    10321 ccccgcctcc gccctgttca aagccaagcc caaatgggtg atggcggcag aattggttga
    10381 aaccacgcgc ctttacgcgc gcgacgtcgc cgttatccag cccgaatgga tcgagcagga
    10441 agccccgcac cttgtccgct accattattt cgagccgcac tgggaacaaa aacgcggcga
    10501 agtcgtcgcc agcgaacgcg taacgcttta cggcctggcc gtattgccgc gccgccccgt
    10561 gtcttacggt aaagttgcgc ccgaagaagc acgtgaaatc tttatccgca gcgcgttggt
    10621 ggcgcaggaa tacgatttga aagcggattt ttttgtccac aacaaaaagc tgattaaaga
    10681 aattaccgaa ctcgaacaca aatcgcgcaa gcaagacgta ttggtcgatg acgaagccct
    10741 gtttgcgttt tatcacgaac ggctgcccga tttttatatg gcggattcgg tttcagaggg
    10801 cctgtgtcct gcaaatccgc agcaaaccac cccctccccc gtgggggaag gctggggaga
    10861 gggcaaaaca gttgccgcac aaaccaactt ttccgcaacc tcagcaagcc ctctccctaa
    10921 ccctctccca caggagaggg aacagagtgc ctcagtttca acggtttcag gcagtctgaa
    10981 aacaatgtct tgcgaagcaa ggctgaattt ctgcgaagct aaaactaaaa ccgaaagcag
    11041 cctgcactcc caaaggctat ctgaaaacta caccccgcca ttttcagacg gcctgcgtcc
    11101 tgcaaacccc cagcaaacca ccccctcccc cgtgggggag ggctggggag agggcaaaac
    11161 agttgccgca caaaccaact tttccgcaac cgcagcaaac cctctcccac aggagaggga
    11221 acagagtgcc tcggctttaa cagtttcaga tgaccccaaa cccaaaaagc agcctgcatc
    11281 tcaaaaaggc cgtctgaaac ctttgcccct tgccgatatc cgcacctttg aagcctggct
    11341 caaaaccgcc gagcgcgaca acccgcgcct gttgttcctc agccgcgacg acctcatgca
    11401 gcacgccgcc gcgcacatca ccgaagaaca attccccaaa cactggcaga ccgcagacgg
    11461 caaattcaaa ctcagctacc gcttcgagcc gcaccacccg ctcgacggcg ttaccctcac
    11521 cctgccgctc accgtgctca accgcatcag ccccgccgcc ctcgaatggc tcgtgcccgg
    11581 catgatacgc gaaaaaatcc agctacaaat caaagcactg cccaaacaaa tccgccgcat
    11641 ctgcgtaccc gtacccgaat tcatcaccca atttttaagc caaaaccccg accgcaacgc
    11701 ccccatcctg ccccagctcg cccaagccat cgccaaaacc gcaggcgaca tccgcatact
    11761 cgagcaaatc aaccaagacg aatgggccgc gttcaggctg cccgaacact gctatttcaa
    11821 cctccgcatc atcgacgacg gcggacaaga gttagccatg ggtcgcgatt taatccaaat
    11881 ccaacaacaa cttggcaaag ccgccaccac caccttccgc gacaacaccc aagaattcga
    11941 gcgtgacaac gttaccgcat gggacatcgg catcctgccc gaatccatca aattcgcccg
    12001 cggcaaacaa cagctcaccg gctacctcgg cctgcaaaaa gaaaaagacg gccgcatcgc
    12061 cctgcgcctg ttcgacacgt ctgccgccgc cggacacgcc cacagattag gcgtgattga
    12121 actcatgaaa ctacaactaa aagaacaagt taaagatctg aacaaaggca tccaaggctt
    12181 cacccaagcc gccatgctgc tcaaacacat caacgccgac accctgcgcg acgacctcac
    12241 ccaagccgtc tgcgaccgcg cctttatcgg cgaagacaag ctgccgcgca acgaaaaatc
    12301 cttcaaagaa caaatcaaac gcgcccgcag ccgcctgccc gccgtcaaag aagccctcag
    12361 ccgctacctg caggaaaccg cagccgccta cgccgaactg aacggcaaac tcggcaaaca
    12421 cccattgacc cacctcctaa gactacgcct gcaaaccctg cttgcacccg gcttcgccac
    12481 ccgcaccccg tgggcacaat ggccgcgcct ccccatctac ctcaaagcca tgaccctgcg
    12541 cctcgaaaaa tacagcagca accccgcccg cgacgcagcc cgcgaagccg atatccaaga
    12601 gctggaacaa atgtggcagg aaaaaactga cggcttggcg aaacaaggac agcccgtttc
    12661 agacggcctc gccgcgttta aatggatgat tgaagaattg agagtgtcgc tgttcgcgca
    12721 ggagttgaag acgccgtatc cggtgtcggt aaagcggctg ttgaagatgt g
//
LOCUS       DAXDZG010000066        12401 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXDZG010000066 DAXDZG010000000
VERSION     DAXDZG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.066"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            100..1866
                     /locus_tag="ACFB78_001642"
                     /pseudo
     CDS             100..1866
                     /locus_tag="ACFB78_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            1970..2638
                     /gene="hda"
                     /locus_tag="ACFB78_001643"
     CDS             1970..2638
                     /gene="hda"
                     /locus_tag="ACFB78_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC1269598.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            2635..3303
                     /locus_tag="ACFB78_001644"
     CDS             2635..3303
                     /locus_tag="ACFB78_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC1269599.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            3308..3595
                     /locus_tag="ACFB78_001645"
     CDS             3308..3595
                     /locus_tag="ACFB78_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating enzyme"
                     /protein_id="HFC1269600.1"
                     /translation="MPSEARFGVQTALRLKSQKPKATGIHPEKQKTQEFIGKNNNLST
                     VIPTKVGILIRPHGNLYFVIPPIPHHPYRFKSAQKSTSRHSHESGNLGRKI"
     gene            complement(3893..4789)
                     /gene="argB"
                     /locus_tag="ACFB78_001646"
     CDS             complement(3893..4789)
                     /gene="argB"
                     /locus_tag="ACFB78_001646"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC1269601.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            4959..6089
                     /locus_tag="ACFB78_001647"
     CDS             4959..6089
                     /locus_tag="ACFB78_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC1269602.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            6163..7014
                     /gene="lgt"
                     /locus_tag="ACFB78_001648"
     CDS             6163..7014
                     /gene="lgt"
                     /locus_tag="ACFB78_001648"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC1269603.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(7099..7761)
                     /locus_tag="ACFB78_001649"
     CDS             complement(7099..7761)
                     /locus_tag="ACFB78_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC1269604.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            7795..7901
                     /locus_tag="ACFB78_001650"
                     /pseudo
     CDS             7795..7901
                     /locus_tag="ACFB78_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7960..9753)
                     /locus_tag="ACFB78_001651"
     CDS             complement(7960..9753)
                     /locus_tag="ACFB78_001651"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC1269605.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            9999..11108
                     /gene="proB"
                     /locus_tag="ACFB78_001652"
     CDS             9999..11108
                     /gene="proB"
                     /locus_tag="ACFB78_001652"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC1269606.1"
                     /translation="MKYKRIVFKVGTSSITRSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            11121..12383
                     /locus_tag="ACFB78_001653"
     CDS             11121..12383
                     /locus_tag="ACFB78_001653"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC1269607.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADAEEKNRALLAMADSLEAAAEDI
                     LAANRLDLKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLER
                     LLVRGRIEAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEARGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPIGLKELTSYKYIVQGTGQVRE"
BASE COUNT         2892 a         3590 c         3151 g         2768 t
ORIGIN      
        1 aagcaatatg ccctgaccgg caaaacttcc ccaaacttca tcaaaaaatc aactgtaccc
       61 gtctttttca gacggcattg ataccgtaag agatttccca tgcaaaaccg gcaaaccgag
      121 ctttattcca cgccgtcttg gctgttgcag accttattga tgattaccgc cgcctcggcg
      181 gtgattctgt ttttcgcgcg caacacgcgc ttggggcggg agtttgccta tatcctgcgc
      241 ctttgcctga caccgaaaag cacggtcaaa gtattgctgc tgattacggc aatggttacg
      301 ctgctgttaa ccgaagtgcg gttgaatgtg ttgagtacct tcatgtccaa aggactttac
      361 gattcgatgc aggatttgaa tgcttcggca ttttggatgt ttgcagcgat gaacgccggc
      421 gtggtactga tacgggcgtt taacaacgtc gttaacgact ttctcgatta aggcttggcg
      481 attaaatggt cggaacggct caatgaagtt ttgacttcgc gctggcttgc cgacaaaaac
      541 tactaccgcc tgcaaatgcg ccgccacgcg ccggacaaca tcgaccagcg tatccaacag
      601 gatgcgcagg aattcatcgc ttcgaccatc gaatttgtgc gcggcatggt caattcggtc
      661 gttacttctc tggaatttgc cgttgttttg tagggcttgg caggcatcct gaccgtgttc
      721 ggcttcgaca ttccgcacgg cattgtttgg tttgtcttta ttttcatgat tttggcgatc
      781 tttatcgcca tgtggatagg caaccctttg attcgttaca attatgaaaa caaaaaactc
      841 aacggcgact accgttattc cctcatcctc gtacgcgacc acgccgaaag cgtggcgttt
      901 tacagcggcg aacaacacga acacggccgg ctttccgacc gctttaaagc cattatccgc
      961 aaccgttggc gcatcgcgcg gcaaagcgtc tgcttaagcg gctttaacga tatgttcacc
     1021 aacggcatca aactcttcct gattattttg caagctccgc gcctgtttgc cggacaaatc
     1081 aaaatcggcg acatccagca gaccgtccaa gccttcgcgc gactgcaaaa cgcgctgtct
     1141 ttcttccgaa tgttctacaa caaattcacc gcctgccacg cccgactgga gcgtctgtac
     1201 gtctttttgc tgagtaccga agaacaacac ggcgcgcagc aacccgagat tagcgaagtt
     1261 tcagacggca ttgcactgga aaacgtcgcc ctgttccgcc acaacggcga agttttattg
     1321 gacggcatca acatcaaact caaaagcggc gattccctgc tgatacgcgg cccgagcggt
     1381 tgaggcaaaa cctcgctgct gcgcgcgctg gcggggcttt ggccgttcgg cagcagcggc
     1441 aaagtcagcc gtccgccgca tcaaggcatc ctcttcctgc cgcaacgccc gtacacggca
     1501 caaggcagcc tgcgcgacgc ggtttgttac cccgatatcg acaaacagca tcccgagtta
     1561 gccgaagcca tgaacacctg ccgcttgggt tatctgattg acaaattgga taaaacagac
     1621 ggctggcagc acaaactctc ccccggcgaa ctgcaacgcg tcgccttcgt ccgcgccctg
     1681 ctctccaagc ccaaaatcgt cctgctcgac gaagccgccg ccgccttgga cgaaccggcc
     1741 gaagcctagc tctaccgcgc cttaaagcaa aaactgcccg acagcatcat cagtatcgga
     1801 caccgaagta cgctcaacgc gttccgcaat atgcacctcg atgtcggcaa cgctgcctgc
     1861 ggctgaacga tgccgtctga agccgttttc agacggcatc gttcaaattg gaaagacacg
     1921 cgaaaacaac gtacaataaa cccgattcca acccatccgg tatgcctttg tgaaccagct
     1981 tattttcgac tttgccgcac acgactatcc gagtttcgac aaattcctcg gcacggaaaa
     2041 tgccgaactg gtctatgtgt tacagcataa acacgacccg tttatctacg tctggggcga
     2101 agaaggcgcg ggcaaaagcc atctgttgca ggcgtgggtc gcacaggcgc tcgaagccgg
     2161 caaaaacgcc gcctatatcg atgccgcctc catgccgctg accgatgccg cgtttgaggc
     2221 agaatacctc gccgtcgatc aagtcgaaaa actgggcaac gaagaacaag ccctgctttt
     2281 ttccatcttc aaccgcttcc gcaacagcgg caaaggcttt ttgctgctcg gttcggaata
     2341 cacgccccag cagctcgtca tccgcgaaga tttgcggacg cgtatggcat actgcctcgt
     2401 ttacgaagtc aagccactaa ccgaccaaga aaaaatcgac gcgctcgcca atatggcggc
     2461 ggcacgccaa gtaaccgtcg attccgaaat tttcgaatac ctgctcaaac actggcggcg
     2521 cgatatggac agcctgatga tgatgctcga cacgctggac aactacgccg taaccatggg
     2581 caaacgcatc accctgccgc tgcttcgcca gcttttgaaa caacaggaaa cccaatgaaa
     2641 aacctcgcca tcttcgacct cgacaacacc ctcatcaaca ccgattccga ccacgcctgg
     2701 ccgcaatacc tcatcaaaaa agggttggtt gacgctgccg aaaccgaagc gcaaaacgaa
     2761 aaattctacc gcgactacca aaacggctgc ctcgacatcg acgcattcct caaactccac
     2821 ctcgccccgc tcgcccgtta cagcaaagaa gagctggcgg agtttcaccg cgaatttatg
     2881 gcggaataca tcatccccca catctcgcct atgcagcgta tgctggtgca gagccaccaa
     2941 atggcaggcg acgaaaccct cgtgatttcc tcaaccaacg agttcatcat cacccccgtc
     3001 tgccgccttt tcggcatcgc caacatgatc ggcacacaac tcgaaaccgg tcccgacggc
     3061 cgctacaccg gcaattacat cggcacgccc agcctcaaag aaggcaaaat cacccgtctg
     3121 aaccaatggc tcgccgaacg cggcgaaacg ctcgaaagct acggcaaaac ctatttttac
     3181 agcgactcca aaaacgacct gccgctgctg cgcctcgtca gcgaacccgt cgccgtcaac
     3241 cccgatgccg aactggaaaa agaagccaaa gaaaaaggct ggccggtttt gaatttcaaa
     3301 tgacgcaatg ccgtctgaag cccgattcgg cgttcagacg gcattgaggc tgaaatccca
     3361 aaaacccaaa gccacaggaa ttcatccgga aaaacaaaaa acacaggaat tcatcgggaa
     3421 aaacaacaat ctttccaccg tcattcccac gaaagtggga atcttgatcc gtccgcacgg
     3481 aaacttatat ttcgtcattc caccaatccc acaccaccct taccggttca aatccgcaca
     3541 aaaatccaca tcccgtcatt cccacgaaag tgggaatcta ggacgtaaaa tctaaagaaa
     3601 ccttttttcc cgataagttt ccgtgccgac aggtctggat tcccgcctgc gcgggaatga
     3661 cgaaatttca aagttatggc gttatcggaa aaacagaaaa tcaaagccgg agaatttatc
     3721 caaaacaacc ggatttcaaa aaaaccaaat tcccggcggg aatgacggat tttaggtttc
     3781 tgttgtggtt tctgtttgct cgggaataac aacttagaaa ttgcccgaaa ccccaaaaac
     3841 agaaaccgga caaacaggtt ttccgccaaa gccggcattt tccgactttg cttcaggcat
     3901 cttcccctct gcctaaaatc atcgacccga taccggcatc ggtaaagatt tccagcaaaa
     3961 gcgcgttggg caacctgccg tcgatgatgt gcgtggcttt cacaccgttg gcggcggctt
     4021 cgaccgcaga agcgattttc ggcagcatac cgccatacag cgtgccgtcg gcaatcagcc
     4081 catcaatccg tttcggcgtg agtttggtca gcagattgcc cgttttgtcc atcacaccgg
     4141 cgatattcgt catcatcaag agtttttcgg cgttcaattc ttccgccaat ttgcctgcca
     4201 ccaaatcggc gttgatgttg aacgcttcgc ctttttcacc tacgccgacg ggggcgacga
     4261 cgggaatgca gccgcgttct atcagccctt taaccaaacc ggtatcgatg ctttccaccg
     4321 taccgacctg tccgatgtcc acgctattct gttcgggcgt atcgaccaaa agtttcttcg
     4381 ccttaatgaa atggtcgtcg cgcccgctca cgccgaccgc gtgccctcca tatgtgttaa
     4441 tcatcgacac gatttccttg ttgacgtgcc cgcccaatac catttcgaca atatccatcg
     4501 tctctttgtc ggtaacgcgc attccttgga caaattcgcc ctttttgccg actttttcaa
     4561 gcatcgcatt gatctgcggc ccgccgccgt gaacgatgac gggatgaatg ccgaccagct
     4621 tcagcagcac gacatcgcgg gcaaaccctt ctttcaaggc aggttcggtc atcgcgttgc
     4681 cgccatactt gatgacggcg accgaaccgg aaaaccggcg gatgtaaggc agtgcttcgg
     4741 caaggatacg cgccttgtcg gcggcggaaa taatgttttc aaactccata acggctccat
     4801 attgttgaca atttcggctt atgttaacgc caaacgccgt ctgaaacaat cttcagacgg
     4861 cgtttggcac accttcgggc aggttgaagc cttgcgatac gggcggtaaa atcgcggttt
     4921 atccggttcg agaaaccggc gacagcaagg actttcccat gattaaaatc agcacccgct
     4981 tcgatgccgg ctcggtcgtc gtcaaagacc tgaccgaccc ttccaacatc cgcctcgccc
     5041 tgcgtcccga caacgcctcc gatttcgcac aatggttcta cttccggctg caaggcgcgg
     5101 cctatcaaaa ctgcatcatg cattttgaaa acgcggcaga agccgcctac ccgaaaggct
     5161 gggaaggcta tcaggcgtgt gcctcatacg accgccgcaa ctggttccgc gtaccgactt
     5221 cctacgaaaa cggcgtactg accgtcaatc atacgccgct gtccaacagc gtgtattacg
     5281 cctatttcga accctattcc gaagagcagc acctcaacct cctcggcgac gcacaaggca
     5341 gcggcctgtg ccgcatcgac gacttgggca gcaccgtgca aggtcgcgac atcaatctgc
     5401 tgaccatagg caaccaagtc gaaagcgaca tgaaaatctg gattaccgcc cgccagcacc
     5461 cgggagaaac catggcggaa tggtttgtcg aagggctgct cggcaggctg ctcgattcgc
     5521 aagacccgac agcacgcacc ctgctcgacc gcgccacgtt ctacatcgtc cccaatatga
     5581 atcccgacgg ttcggcactg ggcaaccttc gtaccaacgc cgccggtgcg aacctcaacc
     5641 gcgaatggga aaacccgact ttggaaaaaa gccccgaagt gttcttcgtg cgcggaaaaa
     5701 tgctggaaac cggcgtggat ttgtttttgg acatccacgg cgatgaaggt ctgccctttg
     5761 tctttgttgc aggaacggaa ggtgtgccga actacaatcc gcgcatttcc gcgttggaag
     5821 cgcagttcaa aaccgccctt ttgaacgcca gccccgattt ccaagacgaa tacggttacg
     5881 aaaaagacgc gccgggcaaa gcgaatatga ccttggcgac caactgggtc ggcaaccgtt
     5941 tcaactgcct tgcctacacg ctggaaatgc cgttcaaaga caacgccaac ctgcccgacg
     6001 acgacttcgg ctggaacggc cagcgttctt tgcggcttgg ggaagcggtg ctttccgcca
     6061 ttttgaacgt cgccggcgat ttgcgctgac actccccact gaaaatgccg tctgcaagcc
     6121 cttttgcgtc tttcagacgg catttgaaaa cgaaagcccg ttatgattat ccatcaccaa
     6181 ttcgatcccg tcctcatcag tatcggcccg cttgccgtcc gctggtatgc cttaagctac
     6241 atcctcggat ttattctttt tacctttctc ggcagaaggc gcatcgcgca aggcttgtcc
     6301 gtttttacca aagaatcgct cgacgacttc ctgacatggg gcattttggg cgtgattttg
     6361 ggcggacgct tgggctatgt cctgttttac aaattctccg actacctcgc ccatccgctt
     6421 gatattttca aggtatggga aggcggaatg tcgttccacg gcggcttttt gggtgtagtt
     6481 attgccatat ggttgttcag ccgcaagcac ggcatcggct tcctcaaact gatggacacg
     6541 gtcgcgccgc tcgttccgct gggtctcgct tcgggacgta tcggcaactt tatcaacggc
     6601 gaactttggg gacgcattac cgacattaac gcattttggg caatgggctt cccgcaagcg
     6661 cattacgaag atgccgaagc cgccgcgcac aatccgcttt gggcagaatg gctgcaacaa
     6721 tacggtatgc tgccgcgtca tccctcgcag ctttatcagt ttgcccttga aggcatctgc
     6781 ctgttcgccg tcgtttggct gttttccaaa aaaccgcgcc cgaccgggca gactgccgcg
     6841 ctttttctcg gcggctacgg cgtgttccgc tttattgccg aatttgcgcg ccaacccgac
     6901 gactatctcg ggctgctgac cttggggctg tcgatggggc aatggttgag cgtcccgatg
     6961 attgttttgg gtatcgtcgg ctttgtccgg ttcggcatga aaaaacagca ctgaccgcct
     7021 gtgaaaaata ccgtctgaaa catggaaatc cggtttcaga cggcattttt acgtttggtt
     7081 cagatgaaaa caaacgcttc aattcttacc gcaacacgcc ttgtatttcc tgccgctgcc
     7141 gcacggacag ggatcgttcc tgccggtttt ttccccttcc ctgcggacgg tttgcggttt
     7201 gttgatgacc gcctgccagt agcggtagat gtctgccagc gcgtaaggca gttcggacgc
     7261 aagttccgcc agctcgcctt cggtgaattg caggcggata gcgccgtttt cctcttcgtc
     7321 gtaaatgccg cccaatgcca tcacggggta aaacagctct tcaaacgctt catcatcggc
     7381 ggcttcaaac caatcggtcg gcacaatgtc caaaccgtaa agataggcgt tgcaccaagt
     7441 gtaaaaatcg ctgccgccct cgccgtcgtc gtagagccac aaatcgggca gctttttatc
     7501 cgacatcgcg gcggttgttt ccatcgccat tgccaaaacc agccgttcga tttcggaacg
     7561 ttcggcggcg gtaaattgcg attcgtcgcc caacacttcg ggcagccagt cgagcggtgc
     7621 caatttgtcc ggcccgctca acagcgccgt cataaaacct tgaacctcgt cgcaacgcat
     7681 cgtgttgcct tgttcgcttt tggcatccaa caattcgccc aaccgccgtt tggatgcttc
     7741 ggtaaatttt cgggaatcca tcattttcct tttcaaatgg gttttgcgcc ctattatagt
     7801 ggattaaatt taaatcagga caaggcggcg agccgcaggc agtacaaata gtacggcaag
     7861 gcgaggcaac gccgtaccgg tttaaattta atccactata tcgccgcaat gccgtctgaa
     7921 gcctttttat gcttcagacg gcatcgtctg ttttcacgct caaatcgtac cgctgccctg
     7981 cgccttcggt ttggcggcac taaattccag cttgctcaaa gcggaaaggt aagctttggc
     8041 ggtcgccgcc aaaatgtcgg tatccgcgcc ctgaccgttg gcgacgcggt tgccgcgcgc
     8101 caaacggacg ctggtttcgc cttggctttc cgtaccttgc gtgacggcgt tgacggaata
     8161 aatctgcaaa gtcgcgccgc tttgcgccac gctctcaatc gctttgaaaa ttgcatcaac
     8221 agggccggag ccggttgcgg aagcgcgttt ttcttcgcct ttgatgccga acacgatgtc
     8281 ggcgcgcggc tcttcgccgg tttcggtgct gattttttga gaaatgaatt tatagctttc
     8341 ggcattcata ttgcccattt cgtccgacac cagcgcgtgc aggtcttcat cgaagatttc
     8401 gcgttttttg tcggcgagtt ctttgaagcg tgcaaatgcc gcgttcagtg cctcttcgct
     8461 ttccaactcg atgcccaaat ccgccagctt ggttttgaag gcgttgcggc cggacaattt
     8521 gcccaagctc aatcggtttg ccgaccaacc gaccgattcg gcagacataa tctcgtaagt
     8581 ttcgcggtgt ttcagcaccc cgtcctgatg gatgcccgat tcgtgcgaaa aggcattcgc
     8641 gccgacaatg gctttattgg gctgcacggg atagcccgta atggtggaca ccagtttgga
     8701 cgaaggcacg atttgcgtgg tatcgatgcc ggtttccaag ccgaacaagt cgtggcgcac
     8761 cttcaacgcc atcacgattt cttcaaccga agcattcccc gcgcgttcgc ccaaaccgtt
     8821 aacggtacat tccacctgac gcgcgccgcc ttttaatgcg gcaagcgaat tggcaaccgc
     8881 caagcccaaa tcgttgtggc agtgtgccga ccagacgact ttgccgccgt tgggcgtttt
     8941 ggcaatcagt tcgcggaaaa attcttcggt tttgtacggg atggaatagc cgacggtatc
     9001 gggaatattg atggtggtcg cgcccgcttc aatcaccgcg ccgcagattt cggcaaggaa
     9061 atcgatttgc gaacgcaacg cgtcttcgca ggaaaattcc acatcgtcgg tgtattcacg
     9121 agcgattttt accgctttga ccgccgcctc aatcacctgc ttcggcttca tcttcagctt
     9181 gtactccata tggatggggc tggtggcgat gaaggtgtgg atgcgttttt tcggcgcggg
     9241 cgcaacggcc ttacccgcct gacggatgtc ccgctcgatg gcgcgggaca atgaacagac
     9301 cgttgatttg gtaatggttt tcgcaatcgc attgaccgcc tcgaaatcgc ccgggctggc
     9361 ggcggcaaaa cccgcttcga tgatgtccgc gcccaatttt tccaactggc gggcaacgcg
     9421 gattttttcc tctttggtca tagcggcgcc gggcgattgt tcgccgtcgc gcagggtggt
     9481 gtcgaaaata ataacgcggt tggcctgtgt catttctgtt ctctccagag attcgttttt
     9541 ttgtaaaaaa gcattcaaat ccagcggtct tccttgcgat tcggcaagcg cggtcaattt
     9601 ttgcagttgc gccaaaggca gcgagccgcg tgtgcgccat ttgtagatgg cggcggtcgt
     9661 cgcggcattt tcaggatcgt gccgtttcaa cgcttcggca agcgcgttca cgccgccgaa
     9721 ataggcgacc agacggtcta tatctaattg catggtagtc cttgtctaaa ataaatcagc
     9781 ccgcagcaga aatatggaca ggattatacg cattttggac aaaacggcaa tattaatttg
     9841 aaaaaatctc aataagttgc tataaatttt attaatttta tacattttgt ctattttatt
     9901 gcagatgcgg cgtgattgtt gacaattgca acagaacagg gcaaattcgg cggtacaagg
     9961 cggcaacgag acaggacaaa ccaacaagga cggaaacaat gaaatacaaa agaatcgtat
    10021 ttaaagtcgg cacatcttcg attacccgtt cggacggcag cctctcgcgc ggcaaaatcc
    10081 aaaccatcac ccgccagctt gccgcattgc atcatgcggg acacgagctg gtcttggtgt
    10141 cttccggcgc ggttgctgca gggttcggcg cgctgggttt caaaaaacgt ccggtcaaaa
    10201 tcgccgacaa acaggcttcc gccgccgtcg ggcaggggct gctgatggaa gaatatacgg
    10261 caaacctgtc ttcagacggc atcgtgtccg cacaaatcct gctcagccgt gccgactttg
    10321 ccgacaaacg ccgctaccaa aatgccggcg gcgcactttc cgtgctgctg caacgccgcg
    10381 cgattcccat catcaatgaa aacgacacgg tttcggttga ggagttgaaa atcggcgaca
    10441 acgacacatt gagtgcgcaa gtggcggcga tgatacaggc agacctcttg gtgctgctga
    10501 ccgacataga cggtctttac accggcaacc cgaacagcaa tcccgatgcc gtacggctgg
    10561 acaaaatcga acacatcaac catgaaatca tcgaaatggc gggcggctcg ggttcggcaa
    10621 acggcacggg cggtatgctg accaaaatca aagcggcaac catcgccgcc gaatccggcg
    10681 taccggtgta tatctgttcc tcactcaaac ccgattcatt ggccgaagcc gccgaacatc
    10741 aggcggacgg ctcgtttttc gtcccccgtg ccaaaggttt gcggacacag aagcaatggc
    10801 tggcgttcta ttccgaaagc cggggcagcg tttatgtgga cgaaggtgcg gaacacgctt
    10861 tgtccgaaca agggaaaagc ctgctgatgt cgggcattgc cggaatcgaa gggcattttt
    10921 cccgtatgga caccgtaacc gtgtacagca aggcaaccaa acagcccctg ggcaaagggc
    10981 gcgtcctttt cggctccgcc gccgccgaag acctgctcaa atcgcgtaag gcgaaaggcg
    11041 tgttcatcca tcgggacgat tggatttcca tcacgcccga aatacgcctg cttctgaccg
    11101 aattttagaa aaggaaaccc atgtcaaaca cacaaaaaca gcttgccctt gccaaagcgg
    11161 caaaaaaatc cgtcaacacg gcggatgcgg aagaaaaaaa ccgcgcgctg cttgctatgg
    11221 cggacagtct ggaagcggcg gcggaagata ttttggcggc aaaccgtttg gatttgaaag
    11281 cggcggcagg caaaattccc gacagcatga ccgaccgcct tttgttggac ggcaaacgca
    11341 tttgcgcgat ggcggacggc atcagggcgg ttgccgcgct gcccgacccc gtgggcgaaa
    11401 tactggaaac ctcgactctg cccaacggct tggaaatcgt caaaaagcgc gtggcgatgg
    11461 gcgttatcgg cattatttac gaaagccgcc cgaacgttac ttccgatgcg gcggctttgg
    11521 cactgaaaag cggcagcgcg gtcgtactcc gcagcggcaa agatgcattt caatccgcac
    11581 gcgccatcgt tgccgccctg aaaacggggt tggcacaaac ccgcatcgac cccgaagccg
    11641 tacagctcat cgaagacaca gggcgtgagg gcagttacga aatgatgagg gcgaaagatt
    11701 atctagacct gctgattccg cgcggcgggg cagggctgat acgggcggtg gttgaaaatg
    11761 ccgtcgtgcc ggtcattgaa acggggacgg gcatcgtcca catttatatc gacaaggacg
    11821 cggattggga caaggcactc cgtatcgtgt acaacgccaa aaccggccgc ccgtccgtgt
    11881 gcaactcgat ggaagtgctg ctggtgcatg aaggcattgc tgccgacttc ctgcccaagc
    11941 tcgaacggct gttggttcgc ggccgtatag aagccggact gccgcccgtc cgcttccgtt
    12001 tggatccgca ggcggcgcgg tatatcggcg gcgaagcggc gggtgcagac gatttcgata
    12061 ccgagttttt agactacatc ctcgccgtga aaaccgtcgc ttcggttgaa gaggcagtcg
    12121 ggcacatcga agcccgcggc acgcaccatt cagacggcat cgttaccgaa aaccgccacg
    12181 ctgcggacta tttcacgacc catatcgatt ctgccgccgt gtatatcaac gcgtccacgc
    12241 gcttcaccga cggcggcgaa ttcggcttgg gttgcgaaat gggcatctcc acgcaaaaac
    12301 tccacgcccg gggcccgata ggtttgaaag agctgacgag ttacaaatac atcgtacaag
    12361 gcacgggaca ggttagggaa taatccgaat tcaaacgtct g
//
LOCUS       DAXDZG010000067        11936 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXDZG010000067 DAXDZG010000000
VERSION     DAXDZG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.067"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..486
                     /gene="def"
                     /locus_tag="ACFB78_001654"
     CDS             <1..486
                     /gene="def"
                     /locus_tag="ACFB78_001654"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC1269608.1"
                     /translation="LQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /gene="fmt"
                     /locus_tag="ACFB78_001655"
     CDS             574..1500
                     /gene="fmt"
                     /locus_tag="ACFB78_001655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC1269609.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB78_001656"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB78_001656"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC1269610.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="ACFB78_001657"
     CDS             2804..3403
                     /locus_tag="ACFB78_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC1269611.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="ACFB78_001658"
     CDS             3403..5523
                     /locus_tag="ACFB78_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFC1269612.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="ACFB78_001659"
     CDS             5516..6793
                     /locus_tag="ACFB78_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC1269613.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB78_001660"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB78_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC1269614.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="ACFB78_001661"
     CDS             8097..8558
                     /locus_tag="ACFB78_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC1269615.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8630..10936
                     /gene="topA"
                     /locus_tag="ACFB78_001662"
     CDS             8630..10936
                     /gene="topA"
                     /locus_tag="ACFB78_001662"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC1269616.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAAAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11157..11285
                     /locus_tag="ACFB78_001663"
                     /pseudo
     CDS             11157..11285
                     /locus_tag="ACFB78_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            11275..11819
                     /locus_tag="ACFB78_001664"
                     /pseudo
     CDS             11275..11819
                     /locus_tag="ACFB78_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2934 a         3289 c         3398 g         2315 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacttgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc agaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg cgatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggaccggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgctgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccgg ttcatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgcaatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtgtt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcccgccc
     8581 gaggccgtct gaaacgccgg catcaaaacc accatccaga gaacgacaaa tggcgaaaaa
     8641 cctattaatc gtcgaatccc cgtccaaagc caaaaccctg aaaaaatatt tgggcggcga
     8701 ttttgaaatc cttgcatcct acggacacgt ccgcgacctc gtccccaaaa gcggcgcggt
     8761 cgatcccgac aacggctttg cgatgaaata ccaactcatc agccgcaacg gcaaacatgt
     8821 cgatgcgatt gttgccgctg ccaaagaagc cgaaaacatc tacctcgcaa ccgacccgga
     8881 cagggaaggc gaagccattt cctggcatct tttggaaatc ctcaaatcca aacgcggcct
     8941 gaaaaacatc aagccgcagc gcgtcgtgtt ccacgaaatc accaaaaacg ccgtgctcga
     9001 tgcggttgcc catcctcgcg aaatcgaaat ggatttggtc gatgcgcaac aagcccgtcg
     9061 cgctctggac tatctggtcg gtttcaacct ctcgccgttg ctgtggaaaa aaatccgacg
     9121 cggtttgagc gcgggccgcg tgcaaagccc tgctttgcgc ctgatttgcg aacgcgaaaa
     9181 cgaaatccgc gcgtttgaag cgcaggaata ttggacggta catctagaca gccacaaagg
     9241 ccgcagcaag ttcaccgcca aactcgccca atacaacggc gcgaaactcg aacaattcga
     9301 cctgccggac gaagccgctc aagccgacgt gttgaaagaa ctcgaaggca aagaggctgt
     9361 cgttactgcc attgaaaaga aaaagcgcag ccgcaacccc gccgcgccgt tcaccacatc
     9421 caccatgcag caggatgccg tgcgcaaact cggcttcacc accgaccgca ccatgcgtac
     9481 cgcccagcag ctttacgaag gtatagacgt agggcagggc gccatcggtc tgattaccta
     9541 tatgcgtacc gacagcgtga atttggctga cgaagcgtta accgaaatcc gccattacat
     9601 tgaaaacaaa atcggcaaag aatatctgcc gagtgccgcc aaacaataca aaaccaaatc
     9661 caaaaacgcc caagaagccc atgaagcgat ccgcccgact tccgtgtacc gcacgcccga
     9721 aagcgtcaaa cccttcctga gtgcagacca gttcaaactc tatcaaatga tttggcagcg
     9781 taccgttgcc tgtcagatga cgcccgccaa attcgaccaa accaccgtcg atattaccgt
     9841 cggcaaaggc gtgttccgag taaccggaca agtgcaaacc ttcgcaggct tcctcagcgt
     9901 ttacgaagaa agcagcgacg atgaagagag cgaagacggc aaaaaactgc ccgaaatgag
     9961 cgaaggcgac aaattgccgg tcgacaaact ctacggcgaa caacacttca ccaccccgcc
    10021 gccgcgctac aacgaagcca cactggttaa agcccttgaa gaatacggca tcggccgccc
    10081 ctcgacctac gccagcatca tctccacgct caaagaccgc gaatacgtta cccttgagca
    10141 aaaacgcttc atgcccaccg acacaggcga catcgtcaat aaattcctga ccgaacactt
    10201 cgcccaatac gtcgattacc atttcaccgc caaactcgaa gaccagcttg acgaaatcgc
    10261 cgacggcaaa cgccgctgga ttcccgtgat ggacaaattc tggaaaccgt tcatcaaaca
    10321 agtggaagaa aaagagggca tcgaacgcgc caaatttacc acgcaggaac ttaacgaaac
    10381 ctgcccgaaa tgcggcgaac acaaactgca aatcaagttc ggcaaaatgg gccgtttcgt
    10441 tgcgtgtgcc ggttatcccg agtgcagtta cacccgcaac gtcaacgaaa ccgccgaaga
    10501 agccgccgag cgcatcgcca aagccgaggc tgaacaagcc gaactcgacg gacgcgaatg
    10561 ccccaaatgc ggcggtcggt tagcgtataa atacagccgt accggcagca aattcatcgg
    10621 ctgcgccaac taccccaaat gcaaacacgt cgagccgctg gaaaaaccga aagacaccgg
    10681 cgtccaatgc ccgcaatgca aaaaaggcaa cctcgtcgag cgcaaatccc gctacggcaa
    10741 actgttttac agttgcagca cctatcccga ctgcaactac gccacttgga acccgcccgt
    10801 cgctgaagaa tgcccgaact gccattggcc ggtcttgacc atcaaaacca ccaaacgccg
    10861 gggcgtggaa aaagtctgcc cgcaaaaaga atgcggctgg aaagaacaaa tcgaaccgcc
    10921 tgcaccgcaa gagtgaagcg gttgggatta agatgaaaga aaatgccgtc tgaaaggggt
    10981 ttcagacggc atttgtaaat tagaaggggc tgtcccagat ggctggggaa atttaaatta
    11041 agtcagaatt atccctatga gaaaagccgt ccaagccggt acaaacaaaa gaaacccatc
    11101 ggactgtttg ccgcaggcgt aactgcaaga acggcagcag ggttggcagc agttaatcaa
    11161 aacacggcag cctgttattt tctatggcga aatagtgttt ggcacatcat cgggaaaact
    11221 tcctttacac gcatttggac gaaatctgcg aaattatgga ggcatacgac atatcgttta
    11281 cgattactta tccgtcaaaa cagtccgcat ttggaaatgt atgacggcga agcaggggcg
    11341 ggtgaaagtt atttccgtaa aggcagacgc ggccgcagtg ccgccggcaa agccgccgta
    11401 ttcgggcttt tgaagcgcaa cggcaaggtt tgtacggtta ctgtcggaca ctcgaaccgc
    11461 tattttattg cctattatcc gcggacgggt gaaacccggc agcattgttt atacggattg
    11521 ttcgtagtta cgatgtatca gattgtaagc ggatttggcc gtttccgtat caatcgcggc
    11581 acacattttg ccgaacggca aaaccatatc aacgcaattg ggaacttttg gaatcgggca
    11641 aacgtcattt gcgcaagttt gacggcattc ccaaagagca tttcgggctg tatttgaagg
    11701 agtgcggacg gcgttttgac aacagtgggg taaaagttcg aatttccatt ttaaaacaat
    11761 tggtaaagca ggatttatcc cggttggccg ggattttaaa aaataaatac attctttaac
    11821 aaaacaaatc atatcccggc aagacaaatc tgataaaatg cttggcgttt attaacaatc
    11881 tttttaataa tttaatctta gatattcgga gttgatatgc atacggccga caaaaa
//
LOCUS       DAXDZG010000068        11470 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXDZG010000068 DAXDZG010000000
VERSION     DAXDZG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.068"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(206..1285)
                     /gene="apbC"
                     /locus_tag="ACFB78_001665"
     CDS             complement(206..1285)
                     /gene="apbC"
                     /locus_tag="ACFB78_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC1269617.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLSMDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1504..1579
                     /locus_tag="ACFB78_001666"
     tRNA            1504..1579
                     /locus_tag="ACFB78_001666"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1537..1539,aa:Lys,seq:ttt)
     gene            1652..2161
                     /locus_tag="ACFB78_001667"
     CDS             1652..2161
                     /locus_tag="ACFB78_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC1269618.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIVATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2502..2942
                     /gene="nadR"
                     /locus_tag="ACFB78_001668"
     CDS             2502..2942
                     /gene="nadR"
                     /locus_tag="ACFB78_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC1269619.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3057..3557
                     /gene="hpaC"
                     /locus_tag="ACFB78_001669"
     CDS             3057..3557
                     /gene="hpaC"
                     /locus_tag="ACFB78_001669"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC1269620.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3573..4268
                     /gene="murU"
                     /locus_tag="ACFB78_001670"
     CDS             3573..4268
                     /gene="murU"
                     /locus_tag="ACFB78_001670"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC1269621.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4302..4732)
                     /locus_tag="ACFB78_001671"
                     /pseudo
     CDS             complement(4302..4732)
                     /locus_tag="ACFB78_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            4907..6583
                     /locus_tag="ACFB78_001672"
     CDS             4907..6583
                     /locus_tag="ACFB78_001672"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC1269622.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6697..7788
                     /gene="ychF"
                     /locus_tag="ACFB78_001673"
     CDS             6697..7788
                     /gene="ychF"
                     /locus_tag="ACFB78_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC1269623.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7826..8065
                     /locus_tag="ACFB78_001674"
     CDS             7826..8065
                     /locus_tag="ACFB78_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269624.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            8062..9934
                     /locus_tag="ACFB78_001675"
                     /pseudo
     CDS             8062..9934
                     /locus_tag="ACFB78_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            9996..11291
                     /gene="tyrS"
                     /locus_tag="ACFB78_001676"
     CDS             9996..11291
                     /gene="tyrS"
                     /locus_tag="ACFB78_001676"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC1269625.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2532 a         3151 c         3200 g         2587 t
ORIGIN      
        1 ctgctccctc ccctgtgggg gagggccggg gagagggcat tctccaagtt gcggcaatct
       61 ttcccaacag cttaaccgcc caaatacaag ccttgcggct tgttgccctc tctccagccc
      121 tctcccacgg ggagagagga ctatgaagcc cgccggcagg cgcggggctt cagacggcat
      181 tgctgttgcg gtttcgggcg cggctttatt cgacgacaat tttagggaac cggctgctga
      241 agtctttgcc tttgtcggca atgcccaggg cgatttggaa tgcggcatcg gtgtagattc
      301 gggcgatggc ggggtgttcg tcgaacagtc gcgccggtgt gccgccgtcc atggcttcgc
      361 gtacgggcag gcttagggga agctgtccga gcagggggac gttgaggcgt gccgccaaat
      421 ctttgccgcc gtccgtgccg aacagtgctt cgctatgccc gcagttggag cagatatgga
      481 cggacatgtt ttccaatacg cccaaaatgg gaatgttgac cttgcggaac atatccacgg
      541 ctttgcgcgc gtctatcagc gcgatgtcct gcggcgtggt tacgatgacg gacccggtta
      601 cggggatgcg ttgggacagc gtgagttgga tgtcgcccgt gccggggggc aggtcgatga
      661 aaagatagtc cacttcgtcc cactcgcttt ggaacatcag ctgctgcaag gcttggctga
      721 ccatcggccc gcgccagacg acggcttggc cggtatcgac gagaaagccg atggacatga
      781 cctgtatgcc gtctgaagat tcgacgggaa tgagtttttg gtttttctga tcgggtttgc
      841 ggtcgtgtac gcccagcatc gtgggctggc tcgggccgta aaggtcggca tcgagcacgc
      901 cgacgcgcgc gcccatgcgc gccattgcgg cggcaaggtt ggcggtggtt gttgatttgc
      961 ccacgccgcc ttttcccgat gcgacggcaa tgatgttttt cacgcctttg atggtggcga
     1021 cgccgggccg gactttgtgt gtgccgattt cggtgtccat ggacagatgg atgtgcgcgc
     1081 cgcctgtttc gggcatcagg gtttcttgta cggcatcggc gagggcggcg gcgatgtgcg
     1141 cgacagggaa accgaaatgc agggcgatgt ggatgccgtc tgaacgctgt ccgaccgaac
     1201 ggacggcctt ttcgccgccg agcgtgcgtg ccgtattcgg aacggcgacg gtgtcgagga
     1261 gggtgcggat gttttgtata ttcatgatgt gtgcctctgt tcgcaagtcg tgtgagggga
     1321 agatagacgg aaagtgtaca tgattttggg cagtttgctt ttttcttgtg cgaactttta
     1381 cacggtatgc ggcggattgt gaaaatccgt tgccaaaata tcccgtgcaa acagcagtct
     1441 gcaattaagc ctgtaaaatt tgcttgacgg cggggaacgg ggctgtataa tgcacggctt
     1501 atcgggtcgt tagctcagcc ggtagagcag cggactttta atccgttggt cgagcgttcg
     1561 aatcgctcac gacccaccag ataaccggaa gccaagtttc gagacttggc ttttttgttt
     1621 gcctgccggg gcgtaccgtt tgcgggggtg tatgaaaaag aaactgcttt cgggcatcaa
     1681 atttgccgtt cggacggcat tggtgttttt gctggtgtcg ctgtttttgg attgggtacg
     1741 caagcccgac gaacctgccg gggcggcagg gcggcctttg accctgctgt cggggcagcg
     1801 gctgactttg ggacagttta gccgggatag gacggtgctg gtgtattttt ggggaagctg
     1861 gtgcggcgtg tgccgttatc agtcgccgat aatcgatgat ttggcggcgg acggcgtgcc
     1921 ggtcgtcggt gtggcggtac gttccggcag cgcgtccgaa gtggcggcat atatggcaaa
     1981 acggggcttg ggctttccga ctgtcagcga tgaggacggg ggtttggcaa ggtcttggcg
     2041 gattgtcgca acgcctgccg tcgttttggt caaaaatggg aaaatggtcc gctatacgac
     2101 gggaatcagc agttattggg gcttgcgcgc acgaattttt caggcggatt ttttcggtta
     2161 acttttgttt ttgtcaaact ttccgctatg tagagaatca aacggcatat gccttgcgcg
     2221 gcggatgcgg cggcttttgt tttttgggaa agagccggcg cgcctgccga tgtaatccct
     2281 tgttgtgatt gtgggaaaaa tagattaaaa tataactatt aaaatatttt cagataggat
     2341 tatcggaatt aaagtctttt atacccggtc gtccgatgag gtttatagcg tattgttgct
     2401 atatgttcgt tttgttatat aacggttgca tcaaaaatta cgcccacagg ctttcccgac
     2461 ggtttgaaag tttgattttc gataacttgg agacttaaac aatgcctacc caatcaaaac
     2521 atgcgtctat caatatcggt ctgatacagg caagggaagc cctgatgacc caattcaggc
     2581 ctattctgaa tcaggcgaat attaccgatc agcaatggcg gattatccgt cttttggcgg
     2641 aaaacggcac attggatttt caggatttgg cgaatcaggc gtgcattttg cgccccagcc
     2701 tgaccggtat cctgacccgc cttgaaaaag cggggttggt cgtccgcctg aaaccttcca
     2761 acgaccaacg gcgcgtttat ctgaagctga cttccgaggg cgagaagctg tatgaggaaa
     2821 tcggcgaaga agtggacgag cgttacgacg ctatcgagga agtgctgggc cgcgagaaaa
     2881 tgctgctgct taaagacctg ttggcagaac ttgccaaaat cgaggatgcg ttgaactcgt
     2941 aatacgccgt aatgcgcgga aacgtccgac cgacggcttt ttgaatcaga actgctgcac
     3001 atgggggatg ccttgtgtgc agcattctta tataggggac ggtttaaagg ggaaaaatgg
     3061 cggatttgca gaaaactttt caaacttcgt tccgtgatgc gatggcatcc tgtgcggcag
     3121 gcgttcatgt catcacgaca gacggtgcgg cagggcgtta cggcattaca atgacggcgg
     3181 ttgcgccggt taccgacgag ccgccgaccg tgatgctgtg cataaaccgg agtgcgcgaa
     3241 tcattccgat cctgtcggaa aacggcagcc tctgcatcaa tatgctggcg gacgaacatc
     3301 aggatgttgc cgaacatttt gccgggctga ccggcctgtc gcccgaagag cggtttgcct
     3361 accatatttg gcatcgcggc aaaacgggac aacttgaaat agaaggcgcg ttggcgcacc
     3421 tgcacgggca tattgtcggc aaacatgaaa tcggcacgca ttttgtgttt tacgtcaggc
     3481 tcgacgaaat caaaaactgc gggtgcaaac gccccgcgct gctgtatttc agacggcagt
     3541 ttaggccttt agactgatat tcggacagat atatgaaagc gatgatactg gcggcaggac
     3601 gcggcgagcg tatgcgccct ttgaccgaca ccactccgaa gccgctgctc gatgtggcgg
     3661 gtaagcctct aatcggttgg cacttgtgcc gtctgaagca ggcggggttt accgaaatcg
     3721 tcatcaacca cgcttggctg ggtcggcaga tagaaaatgc tttgggcgac ggctcggctt
     3781 atggcgtgaa catcgcctat tcgcccgaac ccgcaggcgg tttggaaacg gcaggcggca
     3841 tcgcgcaggc attgccgctg ttgggtgggc agccgttttt ggtggccaac ggcgacgtgc
     3901 tgaccgacat cgattttacc gccgcgtttc agacggcatc gtccctgccc gggcacattt
     3961 ccgcacattt gtggctggtg ggaaatcccc cgcacaaccc cgacggcgat ttttccctgc
     4021 tgcccgacgg cagcgtgcgg ccggaagtat ccggcggcaa cggactgaca ttcagcggcg
     4081 tgggtattta ccgtcctgaa atgtttgacg gaatcgaagc gggcagtgtg gcaaaactcg
     4141 cgcccgtatt gctgaacgaa atgcggcaaa accgcgtgag cggtcagaag catacgggcc
     4201 tgtggctgga tgtcggcacg gtatgccgtc tgaaggaagc gcaagccctt gcagcggctt
     4261 ggaagtgaaa actcggtttc aggcggtatg gcggattcgg tttaacgttt cagtgccaac
     4321 gtcaacacgc cgccgtaacc agccccaagt ctatccattc ctgcgtgttc gggcgttcgt
     4381 ctaagaaaat caccgccatc agcgcgacca agaccaggct gaatttgtcg acgggcgcga
     4441 cttgcggggc tttgcccagt tgcagggctt tgaagtaggc gagccaagat gcgccggtgg
     4501 cgagtccgga caaaaccgga aacgtccggt tgcgccccgt gaagccgttt acgccctgcc
     4561 atttgccggt gtaggtcaaa aacaatacca aggcggcgag gatgaccaag gtgcggataa
     4621 aggcggcgaa atccgaatct atgccctgca agcccatttt ggcgaaaacg gcggtcaatg
     4681 aggcgaagcc tgccgatgcc aatgcccaaa acagccatgc gttgctgccc atgttttctc
     4741 ctttgattgt gaacaatatg aacggtattt ttgttgctgc gtcaaaaatt tcactgcggg
     4801 tttggtgcgg ataacgttat aatatgcctg atattatttt caatccacct gtttgtcgcc
     4861 tgatgctttc agacggcatg tccctcctca tttctaaagg aaaatcatga gcttcaaaac
     4921 cgatgccgaa accgcccaat cctccaccat gcgcccgatt ggcgaaattg ccgccaagct
     4981 gggtttgaac gttgacaaca ttgagcctta cggtcattac aaagccaaaa tcaatcctgc
     5041 cgaagcgttc aagctgccgc aaaaacaagg caggctgatt ttggttaccg ccatcaaccc
     5101 gactccggcg ggcgaaggca aaaccaccgt aaccatcggt ttggcggacg cattgcgcca
     5161 tatcggcaaa gactctgtga ttgctttgcg cgagccttct ttgggtccgg tgttcggcgt
     5221 gaaaggcggc gcggcaggcg gcggctacgc gcaagttttg ccgatggaag acatcaacct
     5281 gcacttcacc ggcgacttcc acgccatcgg tgcggcgaat aacctcctcg ccgccatgct
     5341 cgacaaccat atctaccaag gtaacgagtt gaacatcgac cccaaacgcg tgctgtggcg
     5401 gcgcgtggtc gatatgaacg accgccagtt gcgcaacatc atcgacggta tgggcaagcc
     5461 tgttgacggc gtgatgcgtc ccgacggctt cgacatcacc gtcgcctccg aagtgatggc
     5521 ggtattctgc cttgccaaag acatcagcga tttgaaagag cgttttggca atattctcgt
     5581 cgcctacgcc aaagacggca gccccgttta cgccaaagat ttgaaggcac acggcgcgat
     5641 ggcggcattg ctaaaagatg cgattaagcc caatttggtg caaaccatcg aaggcactcc
     5701 ggcctttgta cacggcggcc cgttcgccaa catcgcccac ggctgcaact ccgttaccgc
     5761 aacccgtctg gcgaaacacc ttgccgatta cgccgtaacc gaagcaggct tcggcgcgga
     5821 cttgggtgcg gaaaaattct gcgacatcaa atgccgcctt gccggtttga aacctgatgc
     5881 ggcagtcgtc gtggcgactg tccgcgccct gaaatacaac ggcggcgtgg aacgcgccaa
     5941 ccttggtgaa gaaaacctcg aagccttggc aaaaggtttg cccaacctgt tgaaacacat
     6001 ttccaacctg aaaaacgtat tcggactgcc cgtcgtcgtt gcgctcaacc gcttcgtgtc
     6061 cgactccgat gccgagttgg cgatgattga aaaagcctgt gccgaacacg gcgttgaagt
     6121 ttccctgacc gaagtgtggg gcaaaggcgg cgcgggcggc gcggatttgg cgcgcaaagt
     6181 cgtcaatgcc atcgacaacc aacctaataa cttcggtttc gcctacgatg tcgagttggg
     6241 catcaaagac aaaatccgtg cgattgccca aaaagtgtac ggcgcggaag atgtcgattt
     6301 cagcgcggaa gcgtctgccg aaatcgcctc gctggaaaaa ctgggcttgg acaaaatgcc
     6361 gatctgcatg gcgaaaaccc aatattcatt gagcgacaac gccaaactct tgggctgccc
     6421 cgaaggcttc cgcatcaccg tacgcggtat cactgtttcc gccggcgcgg gcttcatcgt
     6481 tgcgttgtgc ggcaatatga tgaaaatgcc gggcctgccg aaagttccgg ctgccgagaa
     6541 aatcgatgtg gacgaacacg gcgtgattca cggcttgttc tgaacggttt ttgaaaccgg
     6601 atgccgtctg aagccgtttc agacggcatt ttttcggaac gcgggcggcg gtatgctata
     6661 atccgccgtt aaatttctct attttcagga aaaaccatga gtttgaaatg cggcatcgtc
     6721 ggtttgccca acgtcggcaa atccaccctt tttaacgcgc tgacccaatc gggcatcgaa
     6781 gcggcaaact atcccttctg caccatcgaa cccaacgtcg gcatcgtcga agtgcccgac
     6841 ccgcgtatgg cggagctggc gaaaatcgtc aatccgcaaa aaatgcagcc cgccatcgtc
     6901 gagtttgtcg atattgccgg cttggttgcg ggcgcgagca aaggcgaagg cttgggcaac
     6961 cggttccttg ccaacatccg tgaaaccgat gccatcgtca acgtcgtgcg ctgctttgac
     7021 gacgacaaca tcgtccacgt ttccggcaaa gtcgatccga ttgccgacat cgaaaccatc
     7081 ggcaccgaat tggcgcttgc cgacttggca agtgtggaaa aagccatcgt ccgcgaagaa
     7141 aaacgcgccc gatcaggcga caaagacgcg caaaaactag tcgatttgtg caaaaaactg
     7201 ctgccgcatc tggacgaagg caaacccgtg cgttccttcg gtttggacgc ggaagaacgc
     7261 gcgctgctga agccgctgtt cctgctgacc gccaaacctg cgatgtatgt gggaaacgtt
     7321 gccgaagacg gttttgaaaa caacccgcac ctcgaccgcc tgaaagaatt ggcggcaaaa
     7381 gaaaacgccc ccgtcgttgc cgtctgcgcc gcgatggaga gcgaaatcgc cgaattggaa
     7441 gacggtgaaa aagccgaatt tctcgccgaa atgggcttgg aagaaccggg cttgaaccgc
     7501 ctcatccgcg cgggttacga ccttttggga ctgcaaacct acttcaccgc cggcgtgaaa
     7561 gaagtccgcg cgtggacgat acacaaaggc gacaccgcgc cgcaagccgc cggcgtgatc
     7621 catacggatt ttgaacgcgg cttcatccgc gcccaagtga ttgcctacga tgattttgtc
     7681 tcgctcggcg gcgaagccaa agccaaagaa gccggcaaaa tgcgcgtgga aggcaaggaa
     7741 tacgtcgtgc aggacggcga cgtgatgcac tttttgttta acgtgtaacc caaatgcggc
     7801 aggttttcag acggctttgc cggaaatgcc gtctgaagcc ggttttggtg gttttcgacg
     7861 ttcccatacc gccggaatgc agccgcatca aaataaaatc ccgcccgcat ttccgatttg
     7921 ccctccccga ttcctgcaaa acaaaccgcc tgccctgccg ttacgggaag ccgtccggta
     7981 ttccgaatat cccgaacccc gatacaaaat gacctttcag acggcatttg cacagcccgc
     8041 cgcgtttcaa gtaaaaacat tatgagccaa gccttaccct accgcccgga catcgacaca
     8101 ttgcgcgccg ccgccgtctt gtccgtcatc gtgttccata tcgaaaagga ttggctgccg
     8161 ggcgggtttc tcggtgtcga tatattcttt gtgatttcag gctttttgat gacggcgatc
     8221 ctccttcgcg aaatgtccgg ggggcgtttc ttcctcaaga cattttatat ccgccgcatc
     8281 aaacggattt tgcccgcatt tttcgccgta ttggcggcaa cgctggcagg cggcttcttt
     8341 ttattcacca aagatgattt ctttcttttg tggaaatccg cgctgaccgc cttgggtttc
     8401 gcctccaacc tgtattttgc aagggggaag gattatttcg atcccgcgca ggaagaaaag
     8461 cccctgctgc acatctggtc tttgtcggtc gaagaacaat tttactttgt ctttccgata
     8521 ttgctgttgc ttgtcgcccg caaaagcctg cgcgtacagt tcggcttcct cgccgcattg
     8581 tgcgccttaa gccttgccgc ttcctttatg ccttccgcgc tcgataaata ttacctgccc
     8641 cacctgcgcg cctgcgaaat gctggtcgga tcgctgaccg ccgtgcggat gcggtaccgg
     8701 caacagcgga atcccgccgt cgggaaacgg tatgccgccg tcggcgcatt gttttccgcg
     8761 tgcatactgt ccgcctgcct gtttgcctat tcggaacaaa ccgcctattt cccgggcccc
     8821 gccgccctga ttccctgtct ggctgttgcc gcgctgattt atttcaacca ttacgaacac
     8881 ccgcttaaaa aatttttcca atggaaaatc actgttgccg ccggtttgat ttcctattcg
     8941 ctttatctgt ggcattggcc gatattggcc tttatgcgct atatcggccc ggacaacctg
     9001 ccgccttatt cgccggcggc agcgatcgtc ctgaccctgg cgttttccct gatttcttat
     9061 cactgcatcg aaaagccgtt taaaaaatgg aaaggctcgt tcgcacaatc cgttttatgg
     9121 atttatgcct tgcctatgct cgttttgggg gcgggctcgt ttttcgcgat gaggctgccg
     9181 tttatggcgc aatacgaccg cttggggctg acgcgttcca acacctcctg ccacaacaat
     9241 accggcaaac aatgcctgtg gggggatacg gaaaaacagc cggaactgct ggttttgggc
     9301 gactcccacg ccgaccatta caaaacattc ttcgatgccg tgggcaaaaa agaaaaatgg
     9361 tccgccacta tggtttccgc cgacgcctgc gcctatgtgg aaggctacgc gtcccgtgtg
     9421 ttccaaaact gggccgcctg ccgcgccgtt taccgctatg ccgaagaaca cctgccccgg
     9481 tatccgaaag tggttttggc gatgcgctgg ggcagccaga tgcccgaaaa gccgctccct
     9541 tgcctacgat gccggttttt tccaaaaatt cgaccgtatg ctgcacaaac tctcatccga
     9601 aaaacaagcc gtttacctga tggcggacaa cttggcttcg tcttacaacg tccagcgcgc
     9661 ctatatcttg tcttcacgca taccgggttg ccgccaaaca ctgcgcccgg acgacgaaag
     9721 caccctgaaa gccaatgccc gcatcaggga attggcagcc aaatacccca acgtctatat
     9781 tattgatgcc gccgcctata tccccgcaga tttccaaatc ggcggattgc cggtttactc
     9841 ggacaaagac cacatcaacc cttacggcgg cacagaattg gcgaagcgtt tttccgaaaa
     9901 acaaaggttt ctcgatacgc gccataacca ttgattcgct taaatttgtt acaatcggcg
     9961 gtttgcaaaa ccgctaattt ttttgaaaga gaccgatgag cgtcatccaa gacctgcaat
    10021 cgcgcggcct tatcgcgcaa accaccgaca tcgaagcctt agacgctttg ctgaacgaac
    10081 aaaaaatcgc cctttactgc ggtttcgacc cgacagccga cagcctgcac atcggacacc
    10141 tgctgcccgt attggcattg cgccgcttcc aacaggcggg gcatacgccg attgcactgg
    10201 tgggcggcgc gaccggtatg atcggcgacc ccagcttcaa agccgccgaa cgcagcttga
    10261 attccgccga aaccgttgcc ggctgggtag gaagcatacg cagccaatta acccctttct
    10321 tgagctttga aggcggaaac gccgccatta tggcgaacaa tgccgactgg ttcggcagca
    10381 tgaactgcct cgacttcctg cgcgacatcg gcaagcattt ctccgtcaac gccatgctga
    10441 acaaagaatc cgtcaaacag cgcatcgacc gcgacggcgc aggcatttcc tttaccgagt
    10501 tcgcctattc cctgctgcaa ggctacgact tcgccgagtt gaacaaacgc cacggcgcgg
    10561 ttttggaaat cggcggttcc gaccagtggg gcaacatcac cgccggtatc gacctgaccc
    10621 gccgcctgaa ccaaaaacaa gtgttcggtc tgaccctgcc tttggttacc aaatccgacg
    10681 gtaccaaatt cggcaaaacc gaaggcggcg cggtgtggct gaacgcgaaa aaaacctcgc
    10741 cgtaccagtt ctaccagttc tggctgaaag tcgccgatgc cgatgtgtat aaattcctga
    10801 aatactttac cttcctgtcc atcgaagaaa tcggtgtcgt cgaagccaaa gacaaggcaa
    10861 gcggcagcaa gcccgaagcg caacgcatcc tcgccgaaga aatgacccgc ctgattcacg
    10921 gcgaagaagc ccttgccgcc gcgcaacgca tttccgaaag cctgtttgcc gaagaccaaa
    10981 gccggcttac cgaaagcgac ttcgagcagc tcgccctcga cggcctgcct gcatttgaag
    11041 tttcagacgg catcaacgcc gtcgaagcct tggtcaaaac cggcttggca gcgtccaaca
    11101 aagaagcgcg cggctttgtc aatgccaaag cggttctgct caacggcaaa ccggctgaag
    11161 ccaacaaccc caaccacgcc gccgaacgcc ccgacgatgc ctatctgttg ataggcgaat
    11221 acaaacgttt cggcaaatac accatcctcc ggcgcggcaa acgcaaccac gcgcttttgg
    11281 tttggaaata atccgattgc cgcagaaatg ccgtctgaag ctttcagacg gcatttttat
    11341 caaatgcaaa acaccctgcg cctgccgata tgtcgtcatt tccatgcagg cgggaattca
    11401 aacttgtccg cacggaaact tatcgggcaa aacggtttct tcagttctac gttctagatg
    11461 cccgctttcg
//
LOCUS       DAXDZG010000069        11061 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXDZG010000069 DAXDZG010000000
VERSION     DAXDZG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.069"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            171..2544
                     /locus_tag="ACFB78_001677"
                     /pseudo
     CDS             171..2544
                     /locus_tag="ACFB78_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(2753..3226)
                     /locus_tag="ACFB78_001678"
     CDS             complement(2753..3226)
                     /locus_tag="ACFB78_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC1269626.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGIGKSDSEACEHAL
                     INAARKFQHTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(3357..4712)
                     /gene="yegQ"
                     /locus_tag="ACFB78_001679"
     CDS             complement(3357..4712)
                     /gene="yegQ"
                     /locus_tag="ACFB78_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC1269627.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            5037..5825
                     /locus_tag="ACFB78_001680"
     CDS             5037..5825
                     /locus_tag="ACFB78_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC1269628.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMLKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(5871..6470)
                     /locus_tag="ACFB78_001681"
     CDS             complement(5871..6470)
                     /locus_tag="ACFB78_001681"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC1269629.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            6598..7215
                     /gene="gmk"
                     /locus_tag="ACFB78_001682"
     CDS             6598..7215
                     /gene="gmk"
                     /locus_tag="ACFB78_001682"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC1269630.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            7273..7479
                     /gene="rpoZ"
                     /locus_tag="ACFB78_001683"
     CDS             7273..7479
                     /gene="rpoZ"
                     /locus_tag="ACFB78_001683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC1269631.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            7572..9728
                     /locus_tag="ACFB78_001684"
     CDS             7572..9728
                     /locus_tag="ACFB78_001684"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC1269632.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEQQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDTQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="ACFB78_001685"
     CDS             complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="ACFB78_001685"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC1269633.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         2676 a         2668 c         2997 g         2720 t
ORIGIN      
        1 aaaataatga aacaaacaag gcctcggcat agatgtttgc agagaccttt aattacacga
       61 cacagattta tttgtacggg ttgctgtctt attaaatatt aatgattatc agaaaattta
      121 ttatgaatta actgatggat gaacaatcca ttacatcttg agttgataat atgaaaccat
      181 tacacatgct tcctattgcc gcgctggtcg gcagtatttt cggcaatccg gtcttggcag
      241 cggatgaagc tgcaaccgaa accacacccg ttaaagcaga gataaaagaa gtgcgcgtta
      301 aagaccagct taatgcgcct gcaaccgtgg aacgtgtcaa cctcggccgc attcaacagg
      361 aaatgatacg cgacaacaaa gacttggtgc gttactccac cgacgtcggc ttgagcgata
      421 gcggccgcca tcaaaaaggc tttgctgtgc gcggcgtgga aggcaaccgt gtcggtgtca
      481 gcattgacgg cgtgagcctg cctgattcgg aagaaaactc actgtatgca cgttatggca
      541 acttcaacag ctcgcgcctg tctatcgacc ccgaactcgt gcgcaacatc gaaatcgcga
      601 agggcgctga ctctttcaat accggtagcg gcgcattggg tggcggcgtg aattaccaaa
      661 ccctgcaagg ccatgatttg ctgttggacg acaggcaatt cggcgtgatg atgaaaaacg
      721 gttacagcag ccgcaaccgc gaatggacaa atacactcgg tttcggtgtg agcaacgacc
      781 gcgtggatgc cgctttgctg tattcgcaac gtcgcggtca tgagaccgaa agcgcgggcg
      841 agcgtggcta tccggtagag ggtgctggca gcggagcgaa tatccgtggt tcggcacgcg
      901 gtatccctga tccgtccaaa cacaaatacc acaacttctt gggtaagatt gcttatcaaa
      961 tcaacgacaa gcaccgcatc ggcgcatcgt ttaacggcca gcaggggcat aattacacga
     1021 tttgaagagt cttataacct gaccgcttct tcctggcgcg aagccgatga cgtaaacaga
     1081 cggcgcaatg ccaacctctt ttacgaatgg acgcctgatt caaattggtt gtcgtctttg
     1141 aaggcggact tcgattatca gaaaaccaaa gtggcggcgg ttaacaacaa aggctcgttc
     1201 ccgatggatt attccacctg gacgcgcaac tataaatcag aaggatttgg acgaaatata
     1261 caaccgcagc atggacaccc gattcaaacg ttttactttg cgtatggaca gccaaccgtt
     1321 gcaactgggc ggccaacatc gcttgtcgtt taaaactttc gccagtcggc gtgagtttga
     1381 aaacttaaac cgcgacgatt attacttcag cggacagata tcccgtacta ccagctcgat
     1441 tcaacaccct gtgaaaacca ctaattatgg tttctcactg tctgatcaaa tccaatggaa
     1501 cgacgtgttc agcagccgtg cagatatccg ttacgatcat accaaaatga cgcctcagga
     1561 attgaatgcc gagtgtcatg cttgtgacaa aacaccgcct gcagccaata cttataaagg
     1621 ctggagcgga tttgtcggtt tggcggcgca actgaatcag gcttggcatg tcggttacga
     1681 cattacttcc ggctaccgtg tccccaatgc gtccgaagtg tatttcactt acaaccacgg
     1741 ttcgggtaat tggctgccca atcccaacct gaaagccgag cgcagcacca cccacaccct
     1801 gtctctgcaa ggccgcagcg aaaaaggtac tttggatgcc aacctgtatc aaaacaatta
     1861 ccgcaacttc ttgtctgaag agcagaagct gaccaccagc ggcgatgtcg gctgtactca
     1921 gatgaattac tactacggta tgtgtagcaa tccttattcc gaaaaaccgg aatggcagat
     1981 gcaaaatatc gataaggccc gaatccgtgg tcttgagctg acaggccgtc tgaatgtgga
     2041 caaaatagcg tcttttgttc ctgagggctg gaaattgttc ggctcgctgg gttatgcgaa
     2101 aagcaaactg tcgggcgaca acagcctgct gtccacacag ccgccgaaag tgattgccgg
     2161 tgtcgactac gaagcccgag cgaaaaatgg ggtgtgttct cccgcctgac ttatctgggt
     2221 gcgaaaaagg ccaaagacgc gcaatacacc gtttatgaaa acaagggccg gggtacgcct
     2281 ttgcagaaaa aggtaaaaga ttacccgtgg ctgaacaagt cggcttatgt gtttgatatg
     2341 tacggcttct acaaaccggc taaaaacctg actttgcgtg caggcgtata taatgtgttc
     2401 aaccgcaaat acaccacttg ggattccctg cgcggtttgt atagctacag caccaccaac
     2461 gcggtcgacc gagatggcaa aggcttagac cgctaccgcg cctcaggccg taattacgcc
     2521 gtatcgctgg aatggaagtt ttaatctagt attattggat taattgcctc gttggggatt
     2581 aaagctgtcc gaattgtttt caggaactca ttcggacggc tttttaccgt aaatctgtgt
     2641 gatggggttt tatattgata cagtatccga actcagggca gggagtaatg ttgcaggatt
     2701 aaaaatgccg tctgaaaaat gtttcagacg gcattttttg attgccgata tgttaacgga
     2761 cgacattggc gcgcatgacc gcgcggacgt ggaacgaacc tgcctgacag tcgtattgtc
     2821 cgccttgcag cgattgcttg ccaaagtagc tgtggaagcc ggtaacactg cgcccgccga
     2881 gtttgccggc ggtatgctgg aatttgcggg cggcattgat tagggcgtgt tcgcaggcct
     2941 cggagtcgga tttgccaatg ccgtttgccg cttgccgtgt aactagattc ctgccgtttg
     3001 caaccggtac gccgccgccg aagctgacgg gaatccctga ttccaatacg ccggcggcgg
     3061 tggctgcatt tattgccgcc tgaccgttgc tgcaacggta aatgatgttt ttgccgcctc
     3121 ctgcggtgtc tccgcggata actttgcatg attctccggc cggtgtcccg gcggatgccg
     3181 tgtgcgcgtt tttaagcgcg cggccggatt ggcaggctgc cagcatcagg gcggcggcgg
     3241 agagtatgag gattttttat gtcggatgtc cttgtttgct gtgggaaatc attgccattg
     3301 tacagtaaga ttcaacaaaa tgccgtctga aaacggggtt cggacggcat tgtggctcag
     3361 gggttcaaca cgcgtgcgat gagggctttt tctttgccct gcatattggg gattttgacc
     3421 tgaatgccgt tgcccggggc aacatcgaca ggttggcctt tgcgggtcat ttgttccaat
     3481 ttgatggttt ggttgccgct cgggtggatg atttcgagcg aatcgccgac ggcgaagcgg
     3541 tttttaactt cgatggtcgc ccagccattt tcgtcgattt cggtaacgtg tccgacgtat
     3601 tggctttgtt tggcggtgga atggccgctg aggtagtttt gataatcctg agtttggtgg
     3661 cgttcgagga agccgctggt gtagcctcgg ttggcaaggc cttcgagttc gctcaacagg
     3721 ctgtaatcaa acggacggcc tgcgacggca tcgtgaatcg ctttgcggta ggactgggcg
     3781 acgcgggcga cgtagtagag agacttggta cggccttcga ctttgaggct gtccacaccg
     3841 attttggcga gtttttcgac gacttcgata ccgcgaagat ctttggagtt catgatgtag
     3901 gtgccgtgtt cgtcttccat aatcggcatc atctcgcccg ggcggttgga ttcttcaatc
     3961 aggaaaactt tgtcggcgta gggatggcgt ttttgaccgt tgatgccttc aaagttttgg
     4021 ttggcttctt cttgggcttt ttcaaagttg aaaccttgca gaagctgggc atcgcctgcc
     4081 tcgctttccg tggcgttgtg gactttgtag tcccaacggc aggagttggt gcaggtgcct
     4141 tggttgggat cgcggtggtt gaaatagccc gacaacaggc agcggcctga gtaggcgatg
     4201 cacaatgcgc cgtggatgaa gacttcgagt tcgatgtcgg ggcattcttg gcggatttcg
     4261 gcaatttctt ccatgcccaa ttcgcgtgac aggatgatgc gttcgacacc aatgttctgc
     4321 caaaatttca cgccccagta gttggtggtg ttcgcctgta cggacagatg gatcggcatt
     4381 tccggccatt tttcgcgcac ggtcataatc aaacccggat ccgccataat cagcgcgtcg
     4441 ggtttcatgg caatcagcgg ctccatgtcg gcaacgaagg ttttgagttt ggaattgtgc
     4501 ggcagggtgt tgacggtcaa aaagaatttt ttgttgcgtt cgtgcgcttc tttaatgcct
     4561 tgctcgagga cgtcgagttt ggcaaattcg ttgttgcggg cgcgcaggga gtaacgcggg
     4621 ctgccggcgt aaacggcatc tgcgccgtaa tcgtaggcgg cgcgcattct ttccaatccg
     4681 ccggcgggca ataagagttc gggtgctttc atcgtgtttc cttttcggtt gaaaccccgc
     4741 cctttagggc ggcaggatca gactttattt gggaggggcg taaccccttc cgaatcagga
     4801 cgacatatag ggcggtgctt tatgtgtcgt cctgtgtgtt gaagcatgat ttgtatgctt
     4861 tgtaaaaatg tttcagacgg cattgctgca aatgccgtct gaagggtgtt tgaagatggg
     4921 cggattatcc gccttttcgt gttcgcggtc aattttgccg ggaaatttat aaggatttat
     4981 caagtatttg ccttcttggg atacaatgcg gcagtttgag tgcgagagag aatatgatga
     5041 atcccaaaat cgtgtttttc gacattgacg atacgctgta ccgcaaatat acggatacct
     5101 tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg caaaggtata ttgacggcgt
     5161 tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt cagggacatg atggcggaaa
     5221 cgggtatgga tgccgtggta acgataaacg gacagtttgc gctgctgcac ggtaaaactg
     5281 tgcgcgaagt accgatggat accggtttga tgggcagggt ttgtgcgcat ttggatggct
     5341 tgggcatgga ttatgcgttt gtcggcggag aggggatcgc cgtgtccgcg ctgtcggaat
     5401 gcgtgtgccg cgccttgcag catatcgcca gcgatttttt tgccgataag gattattttt
     5461 caagcaaacc ggtgtatcag atgctggtgt ttgcggagga aaacgaaatg ccgctttggt
     5521 cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg gcacgaggaa gcggtcgatc
     5581 tgctgcctgc gggaatgttg aaaacagacg gcatcagaag cgtggttgaa gcattgggtt
     5641 tggaaatggc agacgtgatg gcgttcggcg acggtttgaa cgatgtggaa atgctgtctg
     5701 aagtcgggtt cggcgtggca atgggtaacg gggaacaggc agcgaaagaa gcggcgaaat
     5761 atgtttgccc cggcgttgat gaagacggcg tgttgagggg cttgcaagat ttgggtgtga
     5821 tttgaacgta taataacaac cctgccggtt tcagacggca gggtcggttt tcagcctttc
     5881 atgcagcctt cgttttgaag cagggtaaat aagggcgcgc cacttgcgcg gatattcttg
     5941 ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt cgacaatttc cccgccgagt
     6001 ttgcggatca gttccagccc ggcaagcatt gtgccgcccg tggcaaccaa atcatcgacc
     6061 agcaggacgc gcgaaccggg tttgacggca tcggtgtgga tttccaccgc agcttccccg
     6121 tattcgagcg cgtagctttg cgatacggtt tcaaaaggca gcttgccttt tttgcggatg
     6181 gggacgaagc cgacgttgag ctggtaggcg agtgccgcgc cgataatgaa gccgcgcgcg
     6241 tccaagccgg caacgatgtc gattttctga tccatatagc ggtaaaccag caaatcgacc
     6301 aaaaggcgga agtattccgc actttgcagg acgggcgtga tgtcgtggaa taagatgcct
     6361 ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa gcgcgccgac actcatagct
     6421 tcgggatgaa ccagcattgc gtgttccaag tttgatgttt ttaacgccat attgtagccg
     6481 tcagcctgcc gcttgccaaa tcaatcaaaa caatagcggc aaaatatggg catcggcaaa
     6541 tatgaaatag ggtgtccgtt gtgatacaat cggttggaaa atatacaggt aaaaaatatg
     6601 tccgcttatc gaaaaggcaa catctttatc atttcggccg cttccggcac gggcaaaacc
     6661 acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc gcgtttccgt gtcgcatacg
     6721 acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt atcactttgt ttccaaagaa
     6781 gagtttgaat cgcttatcgc gcaggaggct tttttggaat acgcagacgt attcggcaac
     6841 tattacggca caagcacgga gggcgtgaat gcgttggcgg cggcaggcta tgacgtgatt
     6901 ttggaaatcg acgttcaggg cgcggcgcag gttcgcaacg cgctgccgga agctgtcggt
     6961 atctttatcc tgccgccttc tttcgacgtg cttgccgcgc gcctcaaagg gcgcgggacg
     7021 gacagtcggg aagttatcca aaggaggctg tcgaaggcaa ggcatgaaat cgaacagtcc
     7081 gtattgttcg actttgtcgt ggtcaatgac gacctggaga aggcggaggg ggatttgctt
     7141 catattgtga atgcctgccg tctgaaaagg tcgcggcaac tgggatttat tgcagatttg
     7201 ttggaaaatt cctaaaaacc gcgaaaatat ccggtttccc aatttaaata tttttgaaag
     7261 aaagcaaata atatggcacg tattaccacc gaagactgta ccggaaaaat ttccaaccat
     7321 tttgacctga cattggtagc ggcccgtcgg gcccgccagc ttgaaaatgg caatacgccg
     7381 cttgtggacg atgtccgcaa taacaaaccg accgttaccg ccttaaggga aatcgcagcc
     7441 gggcatatcg gtacggaact gttgacgcgc aataaataaa ttctgccgga aacgcacgcc
     7501 ggcacactct gccgccgtgc agtccgacgg tttgaaatga aaaaacacat acatcccgaa
     7561 agccgtctga aatgcccgcc ccccaacctt ccgcccctta cgaccccctg accgccgaag
     7621 cgcgtaccct gcttttccat accgcctcct acctcaagcc cgagcaacag gcggagcttg
     7681 aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca aacccgcaaa agcggggatc
     7741 cctacatcac gcatccgatt gccgttgcga cgcagctcgc cctttggcat atggacacac
     7801 agggtctttg tgcaggcgtg atgcacgacg tattggaaga tacgggcgtg acaaaagggg
     7861 aaatggcggc ggtgttcggc aatactgttg ccgagatggt ggacgggctg tccaaactcg
     7921 aaaaactcaa atttgaagat catgcggaac atcaggcgga gagtttccgc aaactgattt
     7981 tggcgatgac caaagatgtg cgcgtcatcg ttgtcaaact tgccgaccgc ctgcacaata
     8041 tgcggacgct cggttcgatg cgcccggaca aacgccgccg gattgcaagg gaaacccttg
     8101 aaatctacgc acagattgcc aaccgcatag gtctgaataa tgcgtatcgg gagcttcagg
     8161 atttatcgtt ccaaaacctg catcccaacc gctacgagac tttaaaaaaa gcgatggaca
     8221 agagccggaa gaacaggcag gacgttgtcg gcaaagtttt gcgcgcgttc ggccagcggc
     8281 tggtaggcgc gaatatagag gccaaaatca aaggcaggga aaaaaacctg tacggcatcc
     8341 atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt tatggatatt tacggtttcc
     8401 gcgtcattgt caacagcatt cccgcctgtt atgccgcact cggcacattg cacaccctct
     8461 atcagcccaa gcccggacgg ttcaaagact atatcgccat tccgaaaagc aacggttatc
     8521 aaagtctgca tacgactttg gtcggcccct acggcttgcc gattgaagtt cagatacgca
     8581 cgcgggaaat ggatgccgtg gcggaaggcg gtattgtcgg ccattggagc tataaatcag
     8641 attccaaaac ggtcgatcag gcagtgctgc acaccaatca atggttgaaa aacattttag
     8701 acttgcaggc aagcagcgcc aatgccattg agtttctcga acacgtcaaa gtcgatttgt
     8761 ttccgaacga aatctacatc cttactccga aaggaaaaat cctaactttg cccaaagggg
     8821 caacgcctgt cgattttgct tatgcggtgc ataccgacat tgggcataaa acagttgccg
     8881 cgcgtgtcaa taatgtgatg atgccgctgc gtacgaagct caaaaccggt gattctgttg
     8941 aaattatcac atccgaacac gccaaaccca atcccgcgtg gttgaatttc gccgtgtccg
     9001 gcagggcgcg cagtgccata cgccaatata ttaaaaatct taaccggcac gatgcggtcg
     9061 ttttgggaga gagcctctta caaaaagcct tgtccagttt gctgcccaaa gatgtcctgc
     9121 tttcagacgg catcaaagaa aaataccttg ccgatctcaa cgacaaacag acttcatttg
     9181 aggaagtgct gtacaacgtc ggtatggggc ataccctgcc tgtttatgtc gccatgcata
     9241 tcgccgaatt ggcaggggag catttcggca gcgaggtcag gctcagttcc attaaagttg
     9301 atggtcagga aagcgggcat attcattttg cagagtgctg ccaccctgtt cccggcgatt
     9361 ccatccgttt gctgttggtt aagggaaaag gcatgattat ccatagggat acctgcccga
     9421 cgttgttgaa gtccgatccc gaacagcagc tggatgcaga ctgggaaaat atgaacgggc
     9481 agaactaccg tgtcgggctt caagtccaat cggaagacag ccacggcctg ttggcattaa
     9541 tggcgcaagc gatttccgat tccggtgcgg acatcgagtc ggtcgaaaca ccgtctaaat
     9601 cccagtcggg aacggaaggt tttgtcgaat tcaaattctt attgaaagtc aagaatttgg
     9661 atcagttgaa tcagattatt caaaatctgc acagcatccc atatatccgc aaagtcatca
     9721 gaagttgaag caggtaaaac gggttgcctg ttaatcaaaa ggcaacccgt tttatggttt
     9781 atccgttcaa ttgcttattt gttcaaatat acggcaagcc tttcaacaat ccgcattgcc
     9841 gcttcatctt tacttgtttc ggggaaagac aactccgcat catcatctat gataacaatt
     9901 tggttggttg tcttgcccat cgcgattgaa acatcattgg caactatcac cggtattttt
     9961 ttcttaatac gtttttcccg cgcatatgcc attacattct ccgtttcagc ggcaaaaccg
    10021 atgcagaacg gcgggttcgg caatgaggca atagaagcca aagtatcggg gttctcatcc
    10081 aattcgatgg ataacggttt ggcattttta tcttttttga atttttgcgt actcctgttt
    10141 ttaaccttat agtctgagac ggcggcaaca gaaataaaag catcttgttt ttcaatcaaa
    10201 cgatgtactg cgcgatgcat atcttcggca ctgacggctt gaaccgtatc ggatatgccg
    10261 aaaggcagcg tggtttgaag ctgtccgtga atcaggctga tttccgcacc ggcggcacgg
    10321 cacgcccgcg ccaaagctac gcccattttt ccgctggagg tatttgtgat gcccctgaca
    10381 gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga ttttcttgtc ccttaaaatt
    10441 ttcggtgtcc acaagtctgg aagcaaatcc agcaattcgg caggttccgg catccttccc
    10501 ataccatttt ctctgcaagc ctgttcgccc aagcccggca tatagacagt aatgccgtct
    10561 gaaaccagtt gtgcgatatt ccgttggttg gcaggattca gccacatttc cacattcatc
    10621 gcgggagcga tggcaagagg gcatttccgt gcggctgcca gattggtcag taggttatct
    10681 gccacgccgt tacagatttt tgccacggta ttcatacttg ccggcgcaat cagaaaaaca
    10741 tccgcattcc gggtcaggtt gatatgttcc ataccgtttg aaccgttgcc gccgtgcgtg
    10801 tcggtcagaa cgggattgcc gctcaaagcc tgaaaagtca gcggagaaac aaattcagtt
    10861 gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt ttttcagcag tcgcaccaac
    10921 tcgcaagact tatacgccgc aatactgccc gttacaccta aaagaatatg tttgcccatt
    10981 ttgacaattt ctaattacga agaaaaaaca gcagccattc tataacaaag tacggataca
    11041 ttagttaccg tttggatacg a
//
LOCUS       DAXDZG010000070        11008 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXDZG010000070 DAXDZG010000000
VERSION     DAXDZG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.070"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(54..1082)
                     /gene="trpD"
                     /locus_tag="ACFB78_001686"
     CDS             complement(54..1082)
                     /gene="trpD"
                     /locus_tag="ACFB78_001686"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC1269634.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRNIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1146..1736)
                     /locus_tag="ACFB78_001687"
     CDS             complement(1146..1736)
                     /locus_tag="ACFB78_001687"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC1269635.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2147..4423
                     /gene="znuD"
                     /locus_tag="ACFB78_001688"
                     /gene_synonym="tdfJ"
     CDS             2147..4423
                     /gene="znuD"
                     /locus_tag="ACFB78_001688"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC1269636.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4963..5742)
                     /locus_tag="ACFB78_001689"
     CDS             complement(4963..5742)
                     /locus_tag="ACFB78_001689"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC1269637.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5896..8745
                     /gene="uvrA"
                     /locus_tag="ACFB78_001690"
     CDS             5896..8745
                     /gene="uvrA"
                     /locus_tag="ACFB78_001690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC1269638.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            8763..9404
                     /locus_tag="ACFB78_001691"
     CDS             8763..9404
                     /locus_tag="ACFB78_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFC1269639.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9408..10541
                     /locus_tag="ACFB78_001692"
     CDS             9408..10541
                     /locus_tag="ACFB78_001692"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1269640.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
BASE COUNT         2902 a         3093 c         2560 g         2453 t
ORIGIN      
        1 cgccaattcc gccgttcaga cggcatttgt tgctttatat caagtttgcc ggcttaggcg
       61 aattgccgtg taaaaccgac aaactcctct tttttcgcct tcgccctacc tgaatcgatg
      121 ccttcccgtg cggcagatat gccgtctgaa agcgaagcag cgatatttcc ggcatataag
      181 gcggcggcgg tattaagcaa tacgatatcg cgcgccgccc cttctttccc gtccagtacc
      241 tcgttcattt tcaacaaaga ttcctgagta ttggcaactt tgatttcatc caaattgcgg
      301 cgggtttcga taccgaaatc ttctgggcgg atgtcgtatt cgctgatttt tccgtctttg
      361 agttcggcaa cgcgcgtttt gcccgtcagt gtaatttcat ccaaaccgcc ctccccgcaa
      421 acgaccaaaa cgtgttttga gccgagttgt tgcaagaccc gcgacaaaat gccgcacaaa
      481 tcggtgtgga acacgcccaa aagctggttc ggcgcgcccg caggattggt taacggaccc
      541 aatatgttga aaatgtttcg gaaaccgagc gaacggcgca ccggggcgac atggcgcatg
      601 gcactgtggt gattgggtgc gaacataaac cctataccgg tctgcctgat actttgggca
      661 atctgttcgg gagtcaggtt gaggtttgcg cccatttgct ccatcacgtc agccgcaccg
      721 ctggaggaag agaccgaccg gcctccgtgt ttggcaacct ttgcgcctgc cgctgcagca
      781 acaaacatcg aagtcgtcga aatattgaag gttttcgcgc catccccgcc cgtaccgacg
      841 atatcgacca gcccgtccgc atcctccagc ggcacttttg acgcaaactc gcacatcacg
      901 gcggcggcgg cggtgatttc ggaaacggtt tcaaccttga tacgcaagcc ggtcaaaatc
      961 gccgcaattt gttcgggcgg cacttttccg ctcatcattt ggcgcatcaa gtcggtcatt
     1021 tcatcgtaaa acaactcgtt attgctgatt aatcgttcga tagcctgttg cggtgtaatc
     1081 attttttgtc ctccgttcaa tattcggacg aaaatgccgc ccgaagggct tcagacggca
     1141 tcacgtcaga ttttttgcgg tttgaagttt tgaaattcaa ctaaaaaatt gttcaacata
     1201 tcatgtccgc gttcggtcaa gagggcttcg gggtggaact gcacgccctc gacggcatat
     1261 tccttatggc gcacgcccat aatttcgccg tcttccgtcc acgccgtgat ttccaagcaa
     1321 tccggcagcg tgccgcgttc gataacgagg ctgtgataac gcgtacaggt aaccggattg
     1381 ggcaaaccct taaacatacc cttgcccgaa tgggacacgg gcgacacctt accgtgcatc
     1441 aaggtttttg cccgtaccac atctccaccg aacgcttcgc ctatcgtctg atgcccgagg
     1501 cacacgccca taatcggcag ccggccggca aaatggcgca tggcttctac tgaaataccc
     1561 gcctccttag gggaacacgg tccgggaccg ataacgagat attgcggatt caatgcctcg
     1621 atttcctcca acgtaatatc atcgttgcgg cgcacggcga cttcctgccc caattctgcg
     1681 aaatactgga cgatgttgta agtaaaactg tcgtaattgt cgataaacaa aagcattttg
     1741 tgattaacct attgatttat aatttgttta ttattaactg catttccgat acccccatcc
     1801 ataccccctt atatcttagc gtgcccgatg cgccctcgtg aatctggcgc agagcctcgc
     1861 gttctgacaa gtacgccatt ttgccacctt ttcgaccgtt cgggaacgta ccgtttttat
     1921 gccctcataa attttggtta tccgatagct aacatatagc ggcgttcgtg ttcacatcat
     1981 taacttccct aaaattaggg ttaatacaaa ttaacgccat taaatatcac aaccgtgtca
     2041 tttgaaaaac ttttgcctat gccctctgag tattctcaaa atcttatgat ataaaaaatg
     2101 caatgttata taataacaaa cttttgaaat gcgacgagaa gccaaaatgg cacaaatcac
     2161 actcaaaccc attgttttat caattctttt aatcaacaca cccctcctcg cccaagcgca
     2221 tgaaactgag caatcggtgg gcttggaaac ggtcagcgtc gtcggcaaaa gccgtccgcg
     2281 cgcgacttcg gggctgctgc acacttcgac cgcctccgac aaaatcatct ccggcgatac
     2341 tttgcgccaa aaagccgtca acttgggcga cgctttggac ggcgtaccgg gcattcatgc
     2401 ctcgcaatac ggcggcggcg catccgctcc cgttattcgc ggtcaaacgg gcagacggat
     2461 taaagtattg aaccatcacg gcgaaacggg cgatatggcg gacttttctc ccgatcacgc
     2521 cattatggta gataccgcct tgtcgcaaca ggttgaaatc ctgcgcgggc cggttacgct
     2581 cttgtacagc tcgggcaatg tggcggggct ggtcgatgtt gccgatggaa aaatccccga
     2641 aaaaatgcct gaaaacggcg tatcgggcga agccggattg cgtttgagca gcggcaattt
     2701 agaaaaactg acatccgcag gcatcaatat cggactgggc aaaaacttcg tgctgcatac
     2761 cgaaggcttg taccgcaaat cgggcgatta cgccgtaccg cgttaccgca atctgaaacg
     2821 cctgcccgac agccatgccg attcgcaaac gggcagcatc gggctgtctt gggtaggcga
     2881 aaaaggcttt atcggcgcag catacagcga ccgtcgcgac cgctacggcc tgcctgccca
     2941 cagccacgaa tacgatgatt gccacgccga catcatctgg caaaagagtt tgatcaacaa
     3001 acgctatttg cagctttatc cgcacttgtt gaccgaagaa gacatcgatt acgacaatcc
     3061 gggcttgagc tgcggcttcc acgacggcga cggtgcacac gcacacaccc acaacggcaa
     3121 accgtggata gacctgcgca acaaacgcta cgaactccgc gccgaatgga agcagccatt
     3181 ccccggtttt gaagccctgc gcgtacatct gaaccgcaat gactaccacc acgacgaaaa
     3241 agcaggcgat gcagtagaaa acttcttcaa caacaaaaca cacaacgccc gtatcgagtt
     3301 gcgccaccaa cccataggcc gtctgaaagg cagctggggc gtgcaatatt tgggacaaaa
     3361 atccagcgcg ctttccgcca ttcccgaaac cgtccaacaa ccgatgttga ttgacaacaa
     3421 tgtccgccat tacagctttt tcggtgtaga acaggcaaat tgggacaact tcacgcttga
     3481 aggcggcgta cgcgtggaaa aacaaaaagc ctccatccgg tacgacaaag cattgattga
     3541 tcgagaaaac tactacaacc agcccctgcc cgacctcggc gcgcaccgcc aaaccgcccg
     3601 ctcgttcgca ctttcgggca actggtattt cacgccacac cacaaactca gcctgaccgc
     3661 ctcccatcag gaacgcctgc cgtcaacgca agaactgtac gcacacggca agcacgtcgc
     3721 caccaacacc tttgaagtcg gcaacaaaca cctcaacaaa gagcgttcca acaatatcga
     3781 actcgcgctg ggctacgaag gcgaccgctg gcaatacaat ctggcagcct accgcaaccg
     3841 attcggcaac tacatttacg cccaaacctt aaacgacgga cgcggcccca aatccatcga
     3901 agacgacagc gaaatgaagc tcgtgcgcta caaccaatcc ggtgccgact tctacggcgc
     3961 ggaaggcgaa atctacttca aaccgacacc gcgctaccgc atcggcgttt ccggcgacta
     4021 tgtacgaggc cgtctgaaaa acctgccgtc cctacccggc agggaagatc cctacggcaa
     4081 acgtcccttc atcgcgcagg acgaccaaaa cgccccccgc attccggctg cgcgcctcgg
     4141 cttccacctg aaaacctcgc taaccgaccg tatcgatgcc aatttggact actaccgcgt
     4201 gttcgcccaa aacaaactcg cccgctacga aacgcgtacg cccggacacc atatgctcaa
     4261 cctcggtgca aactaccgcc gcaatacgcg ctatggcgag tggaattggt acgtcaaagc
     4321 cgacaacctg ctcaaccaat ccgtttacgc ccacagcagc ttcctctctg atacgccgca
     4381 aatgggccgc agctttaccg gcggcgtaaa cgtgaagttt taaaatcgga caggcaaaca
     4441 aaaaaggttt tcagacggca ttctgaaaac ctttttttct ttttaatcaa cttgttagga
     4501 aatatggcaa agacaaggaa caaaccactg tgccatcatt cccattttct ttgtttcatc
     4561 attcttgctt tcacgccgtc attctcgcaa aagcagaaaa atcaaaagca aaaacccaag
     4621 atcccatcat tcctgcgcag gcgggaatcc aattcattcg gtttcagccg tttccgataa
     4681 attaccgtaa cgttaagttc ccggattccc actttcgtgg gaatgacggc gtaaaggttt
     4741 ctgtttttta agttttaggt aactaatgag tcgtcattcc cgcgcaggcg ggaatccaat
     4801 tcgttcggtt tctgttattt ctgataaatc accgtaacgt taagttcccg gattcccatt
     4861 ttcgtgaaca taacggtgtg aacattagca aaaattcaaa aaaccaaact tcctcattcg
     4921 tgtgaatgat ggtactgata ttttctattt cagttcgcaa aattaatctt tcaacacatc
     4981 ttgcacagcc gcctgaatct tagccgccgc cgcttcgatt tgctgctgtt cggcagatgg
     5041 gtttgccgct gtttcaaccg aagcagcttg tgaaacagga tcggcttgag aatcagtcag
     5101 cggcaaacgc gccaatacgg tttttacgcc ggttacttta tcaccaatcg ccacttgcgc
     5161 ctgcgcatcg acaggcagat acatatccac gcgcgaaccg aagcggataa agccatagcg
     5221 ttcgccgcgg gacagtttcg cacctgcttg ggtgtaacac aaaatacggc gtgccaccag
     5281 accggccact tgaacaaaag taatttcacg accggaagcc gtagtcgcca aaaccgcgtt
     5341 gcgttcgttt tccgtgctgg ctttgtccaa atccgcattc acgaatttgc ctttgttata
     5401 gaccactttc gttaccgtac aatcggcagg cgatttttgc gaatgcacgt tgaacacgtt
     5461 cataaaaata ctgattttca aagcatcgac atcacgatac ggatcgcgtg cgcgctcgac
     5521 caccacgata cggccgtcaa ccggactcaa caccgcttca ggattttgcg gaatttcacg
     5581 cgcagggtcg cggaaaaact gcaatgcaaa tacggtaaac acccaaaacg gcaaagacca
     5641 ccagccgcag cacatcgaca ccagcaggct caaagccaaa ccgccgccga taatcggcca
     5701 gccctcacgg gcgataatcg ggtggggata aagacggttc atttgtcatc cttttattca
     5761 aatatattaa atagcgacat tataacggaa tccgccgcgt tccgccgaca aatgccgtct
     5821 gaacgcggct tcagacggca tccgagaaag tgctacaata tcggttttcc tttatttttt
     5881 caggcggcat ccattatgtg caaccatcat ccccgacatt cgcacgacaa cgacaccatc
     5941 cgcattcgcg gcgcgcgcac gcataatttg aaaaacatcg atttggacat tccccgccac
     6001 aagctcgtgg tggtaacagg attgtcaggc agcggcaaat catcgctggc gtttgatact
     6061 ttatatgccg aagggcagcg gcgttatgtc gagagcctgt ctgcctatgc gcggcagttt
     6121 ttgcagatga tggacaaacc cgatgtcgat ttgattgaag gcctgtcgcc tgcgatttcc
     6181 atcgagcaga aatccaccag ccacaatccg cgttccaccg tcggcacggt aacggaaatc
     6241 cacgactacc tgcgcctttt atacgcccgt gtcggcacgc cgtattgccc cgaacacaag
     6301 ctgccgctat ccagccaaac cgtatctcaa atggtcgatg ccgtattaaa actgccggaa
     6361 gatacgcgcg tgatgattct ggggccggcg gtgcgcgagc gtaaaggcga atttgtcgat
     6421 ttctttgccg acttgcaggc gcaaggcttt gcccgtgtgc gcgtggacgg cgaagtctat
     6481 cagcttgacg aagtaccgaa gctggaaaaa aacatcaaac acaatatcga cgtcgtcatc
     6541 gaccgcgtga aagtgaaagc cgacatcaaa caacgactgg cggaaagttt tgaaaccgcg
     6601 ctgcgccacg gcaacgagcg cgcgctggcg atggaaatgg acagcggcga agaacattgg
     6661 ttttccgcgc gttttgcctg ccccgtgtgt tcgtacagcc tgcccgaatt ggaaccgcgc
     6721 ctcttttcgt tcaacaaccc gatgggttcc tgcccgactt gcgacggctt gggcaacacc
     6781 aatttcttcg accccgaaaa agtagtcgcc catcccgaat tgtcgctggc aaccggcgcg
     6841 attgacggtt gggacaaacg caaccaattc tatttccaaa tgattcaatc gctggcgcat
     6901 cattataaat ttgatgtcaa cgtcgcttgg gaaactttgc ctgaaaaagt caaaaaagtc
     6961 gtgttgcacg gctcgggcaa agaagtcatt gatttcacct acctgtccga acgcggcacc
     7021 accttcaacc gcagccacgc cttcgagggc atcatcccca atctcgaacg ccgctaccgc
     7081 gaaaccgaca gcgaaaccgt gcgcgaaaaa ctgcgcgagt accaaaatca ccgtgcctgc
     7141 ccgagctgcg gcggcgcacg tttgcgcaaa gaagcacgct acgtttacgt cggcggcgaa
     7201 ccgttgcacg aagtctccgc ctggccgctg accaaaaccc accgattctt cgaaacgctg
     7261 gatttggacg gcaacaaaaa acagatcgcc gaaaaaatcc tcaaagaaat caccgagcgg
     7321 ctcggcttct tgattaacgt cgggctggat tacctgaatt taagccgttc cgccgaaacc
     7381 ctttccggcg gcgaagccca gcgcatccgc ctcgccagcc aaatcggcag cggcctgacc
     7441 ggcgtgatgt acgttttgga cgaaccctcc atcggcctgc accagcgcga caacgaccgc
     7501 ctgctcgcca ccctcaaacg cctgcgcgat ttgggcaaca gcgtaattgt ggtcgaacac
     7561 gacgaagacg ccatccgcga agccgatttc gtcgtcgata tgggccccgg cgcgggcgaa
     7621 cacggcggca acgtactgat tgccgacacg cccgaaaatg tcgccaaatg cgaaaaatcc
     7681 gttaccggac aatacctcgg cggcaaaaaa tccattgccg tgccgtctga acgcacgccc
     7741 gtcaatcccg accgaatgct cgtcctcaaa ggtgcgcgcg gcaacaacct caaaaacgtc
     7801 accctcgagt tgccgctcgg tttgattacc tgcatcaccg gcgtatccgg cagcggcaaa
     7861 tccaccctga ttaacgacac cctcgccaaa atcaccgccc gcgaactcaa ccgcgcccaa
     7921 gaagaacccg ccccatacga cgacatccgc ggcctcgaac acctcgacaa agtcatcaac
     7981 gtcgaccaat cccccatcgg ccgcaccccg cgctccaacc ccgccaccta caccggcctg
     8041 tttaccccca tccgcgaact cttcgccggc gtacccctct cgcgcgaacg cggctacaac
     8101 gtcggcagat tctccttcaa cgtcaaaggc ggccgctgcg aagcctgcca aggcgacggc
     8161 gtaatcaaag tcgaaatgca cttcctgccc gacgtgtacg tcccctgcga agtctgccac
     8221 ggcaaacgct acaaccgcga aaccctcgaa atccaataca aaggcaaaaa catcagccaa
     8281 gtcctcgaca tgacggtcga agaagcccgc gaatttttcg acgccgtccc caccgtatcg
     8341 cgcaaactgc aaaccctgat ggacgtaggt ctcggctaca tccgcctcgg ccaatccgcc
     8401 accaccctct ccggcggcga agcccagcgc gtcaaactcg ccttggaact ctccaaacgc
     8461 gacaccggca gaacgctcta catcctcgac gaacccacca ccggcctgca cttcgccgac
     8521 atcgccctgc tgctggaagt cataggccgt ctgaaaggca aaggcaactc gattgtgatt
     8581 atcgagcata atctggatgt aattaaaacc gcggattgga ttgtggactt agggccggaa
     8641 ggcggcgatg gtgggggtaa ggttatcgcg aaaggtagcc ctgaggaagt ggcgaaggtt
     8701 aaggggagtt ataccgggaa gtatttgaaa gtggttttga agtaaacgaa cggatataga
     8761 aaatgaacaa taattttgac cgcagcaagc atatctatca taacgaagct tttgtagaac
     8821 tggttaaaga tgccatacgc ttttttcatg gtacgcctgt tcatacactt cctccaccca
     8881 aacagttcaa aggtgcaggg gtttatgcga tttactatat cggtaacaat cctttgtaca
     8941 aacaatatgc ggactggaac agattatcct acaatgcgcc aatttatgtt ggcaaggccg
     9001 tccccaaagg ctggcggcaa gcaagaaatt ctgataatgc gctaaaccaa tcaaccgaac
     9061 tgttccaccg gttgaaagaa cacagcagga gtattgccgc tgtttctgat ttagatcctt
     9121 ccgatttcat gtgcagattc gttatctttg aaggtgccgg ttctgacatg ataggcacaa
     9181 tcgaagctgc cctgattaaa ttacacaaac ctctatggaa ctcttgtgtc gatggtttcg
     9241 gaaaccacga ccccggaaaa ggccgttacg aacaagccaa atcggattgg gatgtccttc
     9301 atagcgggcg ggtttgggca gataggctaa acggcatacc gaatagctat gagtccatac
     9361 ttgaaaatat caacacacat ttagagataa taaagagaaa ataacctatg aagtctttgg
     9421 aaattttttc cggcgcgggc gggttggcaa aaggccttga acttgcagga ttccaacatg
     9481 catcttttat tgaactcaat aaagatgcat gcaattcttt aagaagcaac tttaacccca
     9541 agcttgttta tcagggagat gtggcagatt ttgatctttc cagtcaagaa ggcatagaag
     9601 tcattgcagg cggtcctcct tgccagccct tttcattggg cgggaaacac cttgcccatg
     9661 aagacagaag ggatatgttt cctcatgcgg tcagatatgt ggagtattac cgccccaaag
     9721 cttttatctt tgagaatgta aaaggacttt taagaaaatc attcgctgat tattttgaat
     9781 atatcctact tcggcttact tatccgaatc ttggtatttt gcagaatgaa gattggaaag
     9841 gccatctgac acggctgaaa gaaattgaat tcaatttata taaaggaatc aaatataagg
     9901 tttcatatca attgttgaat gcagctgatt acggagtgcc gcaaaaacgc gaacgcgtgg
     9961 ttatcgtagg tatccgggcc gatttggata ttgattggaa attcccaaaa agaacacatt
    10021 ccgaagacag gttgaattgg gaaaagtatg taacggggga atactgggaa aaacacaacg
    10081 aacccaaaag attcaataaa gatattgctg aaaagttaca aaaaaaatac ggtatattcg
    10141 aaccagaaaa aaaaccttgg caaacggtaa gggatacctt gtccgacatc ccgcatcctt
    10201 tggggaatca taaaattaca ggacatgaat atagggatgg cgcaagaatt tatcccggac
    10261 acacaggaag cgggatagac gaaccgtcca aaaccattaa agcaggtggg catggcgttc
    10321 ccggcggaga aaatatgatt cgttatgatg atggaacagt cagatacttt accagctatg
    10381 aagcaaaact tcttcaaaca ttccctgaag aatttgtcat ttctggagct tggggagaag
    10441 caatgcgaca aattggcaat gcggttcctg tcaaattgtc ggaaatttta ggcaaacatc
    10501 tgatgggggt gttgtcggag aaaagcagcc tgcacaacta acccgcccac tgcgtgtgtg
    10561 cggtacgcac gcggttgctt gattgacggt ttgccgtttc aacttttaac ttcgttgaag
    10621 ctagtgcttt cagacggcct ccaatgtcgt ttggaaacat caggtccaac aaagttaaac
    10681 cttaaacaac aaaccattcc ttctccaaca cccaaaccac aaacaaaaat cctatggcgc
    10741 gttatcgcag aaacttcatt gccggcggca cattcttttt caccgtcaaa cccgccgccc
    10801 cgaaatcgcg tctgcttgtc ggacatatcg gcttcgcgtg cggcttatat ggatgtgcaa
    10861 aaacaatatc cctttgaaac cgtcgccgta tgcgtgctgc cgaaccatat tcacgccatt
    10921 tggacgctgc cgcccgacga tgcggattat tccctactcc ggcggctgat taaaaccaaa
    10981 ttctccgcat attcccctca tactaaaa
//
LOCUS       DAXDZG010000071        10500 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXDZG010000071 DAXDZG010000000
VERSION     DAXDZG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.071"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(67..1305)
                     /locus_tag="ACFB78_001693"
                     /pseudo
     CDS             complement(67..1305)
                     /locus_tag="ACFB78_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1485..2858)
                     /locus_tag="ACFB78_001694"
     CDS             complement(1485..2858)
                     /locus_tag="ACFB78_001694"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC1269641.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4076
                     /gene="dusB"
                     /locus_tag="ACFB78_001695"
     CDS             3072..4076
                     /gene="dusB"
                     /locus_tag="ACFB78_001695"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC1269642.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4089..4238
                     /locus_tag="ACFB78_001696"
     CDS             4089..4238
                     /locus_tag="ACFB78_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269643.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4265..4504
                     /locus_tag="ACFB78_001697"
     CDS             4265..4504
                     /locus_tag="ACFB78_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC1269644.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFB78_001698"
     CDS             4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFB78_001698"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC1269645.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5094..5963
                     /locus_tag="ACFB78_001699"
     CDS             5094..5963
                     /locus_tag="ACFB78_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC1269646.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            6616..6750
                     /locus_tag="ACFB78_001700"
     CDS             6616..6750
                     /locus_tag="ACFB78_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269647.1"
                     /translation="MPSERIFRSVLRLVGVSSDTVKRKFAGAGLSYAGKAAGESLYCL
                     "
     gene            6866..7660
                     /locus_tag="ACFB78_001701"
     CDS             6866..7660
                     /locus_tag="ACFB78_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC1269648.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDTGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            7885..10488
                     /gene="pepN"
                     /locus_tag="ACFB78_001702"
     CDS             7885..10488
                     /gene="pepN"
                     /locus_tag="ACFB78_001702"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC1269649.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRTAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFNLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         2401 a         2749 c         2983 g         2367 t
ORIGIN      
        1 tgaatggtga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgccttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgcttaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 cacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcagcaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg ggcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgtgc
     3901 gcatcgcacg caaacacata ggctggtaca tcggcgaaat gcccgacggc gaacaggcgc
     3961 ggcgtgaaat caaccgcttg gacaatgcgg cggcgcaata cgacacactt gccggttatc
     4021 ttgaaaggct tgccggaaaa accgaccgtt gggcgtgcgg ctatcgggaa gggtagggca
     4081 gtattgccat gccgccgttc gggttcggac ggcatctgtc tgcatggttc ggaggtcggg
     4141 cggaatcccg gccggcgaat gtcgaaaaca ataaatgccg tctgaaaacg agtggaacgg
     4201 gtttcgccaa aaggctttca gacggcattc cgtcttaaaa acatttcaat caaaaggata
     4261 tgcgatgaac cctgcgactg cggacattgc gcaatgtatc gagcagaact tgaaccaata
     4321 tttcaaagac ctgaacggca ccgaaccttg cggcgtgtac gatatggtac tgcatcaggt
     4381 ggaaaagccg ctgctggtgt gcgtgatgga gcaatgcggc ggcaaccagt ccaaagcctc
     4441 cgtgatgctg ggactgaacc gcaatacctt gcgtaagaaa ctgattcaac acggtttgct
     4501 gtgaatatgt cggcaaccgt ccgtatcttg ggcatcgacc cgggcagccg cgtaacgggt
     4561 ttcggcatca tcgatgtcag ggggcgcgat catttttacg tcgcctccgg ctgcatcaaa
     4621 acgcctgccg atgagcctct ggcagacagg attgccgtga ttgtgcggca tatcggcgaa
     4681 gtcgttgccg tttacaagcc gcaacaggcg gcggtggaac aggtgttcgt caacgtcaat
     4741 ccggcatcga cgctgatgct cggtcaggcg cgcggagcgg cattggcggc tttggtcagc
     4801 cataagctgc ccgtttcgga atacacggcc ttgcaggtca aacaggcggt ggtcggcaaa
     4861 ggcaaggcgg cgaaagaaca ggtgcagcat atggtggtgc aaatgctggg actttcggga
     4921 acgccgcagg cggatgcggc ggacggtctt gccgtcgcgc tgacccacgc cttacgcaac
     4981 cacgggcttg ccgccaaact caatccttcg gggatgcagg tcaagcgcgg aaggtttcaa
     5041 tagtttcaga cggcatttgt attttgccgc ctgaaaagaa aatgtgtacc gagatgaaat
     5101 ttatattttt tgtactgtat gttttgcagt ttctgccgtt tgcgctgctg cacaagattg
     5161 ccggcctgat cggttcgctt gcctaccttc tggtcaaacc gcgccgccgt atcggcgaaa
     5221 tcaatttggc aaaatgtttt cccgaatggg acgaagaaaa gcgtaaaacc gtgttgaaac
     5281 agcatttcaa acacatggca aaactgatgc tcgaatacgg cttatattgg tacgcgcctg
     5341 ccaaatgcct gaaatcgctg gtgcgctacc gcaataagca ttatttggac gacgcgctgg
     5401 cggcggggga aaaagtcatc atcctgtacc cgcactttac cgcgttcgag atggcggtgt
     5461 acgcgcttaa tcaggatgtc ccgctgatca gtatgtattc ccaccaaaaa aacaagatat
     5521 tggacgaaca gattttgaaa ggccgcaacc gctatcacaa cgtcttcctt atcgggcgca
     5581 ccgaagggct gcgcgccctc gtcaaacagt tccgcaaaag cagtgcgccg ttcctgtatc
     5641 tgcccgatca ggatttcgga cgcaacaatt cggtttttgt ggattttttc ggcattcaga
     5701 cggcaacgat taccggcttg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg
     5761 ccattcccgt ccgcgaggcg gacaatacgg ttacattgca attctatccc gcttggaaat
     5821 cctttccgag tgaagacgcg caagccgacg cgcaacgtat gaaccgcttt atcgaagaac
     5881 gcgtgcgcga acatccggaa caatatttct ggctgcacaa gcgtttcaaa acccgtccgg
     5941 aaggcagccc cgatttttac tgactacata aaattacaaa acaaatcagg cgtttcagat
     6001 caaaaacccc gattgttttt gggaatttga aacccgggtt gtacaaacag gatttgccgg
     6061 acggttttaa cggttcagtt gtttgtaaaa acaatgcttt tttaaaattg acaaaaaacg
     6121 aaatcggttt taaaggctta ttccgagaac aaaggggagt ggatgccgaa aacccggtta
     6181 atatattata gtggattaac aaaaaccaat acggcgttgc ttcgccttag ctcaaagaga
     6241 acgattccct aaggtgctga agcaccaggc gaatcggttc cgtactattt gtactgtctg
     6301 cggcttcgcc gccttgtcct gatttttgtt aatccactat aaaattaaat ttgtttaaaa
     6361 acataaagtt gtaaacaagt atctcatata agcctttttc attaaacaga tagtcagata
     6421 ttttgtgcta aaaatttata taatatttaa attaatatca agttataaaa atatatggaa
     6481 ttttattttg tttatttata attttaagca ataatctaaa tcaggcattt ttattcccct
     6541 gttttaaaaa atatttggca aggtgtgaaa aaaggcgtac attccgctac acagaattac
     6601 agacgcagcg gagcaatgcc gtctgaaagg attttccggt cagtcttgcg gttggtcggg
     6661 gtttcatcgg atacggtgaa acgaaagttt gccggcgcag ggttgagcta cgcgggtaaa
     6721 gccgcaggcg aaagcctgta ttgtttgtga agcgtaaatc tctgatttga ggtattgggg
     6781 caatcctgtg ggggttgcct ctttttttat ccgcctttta atgacacaat aggcgcaagt
     6841 ctgtttttga atgcaaggta ctgccatgaa tacgattttg gctttcgata ttgaaaccgt
     6901 acccgatgtg cagggtatcc gtacgttgta tgacttgccg tcttccctgc cggatgacga
     6961 agtggtgctg ttcgcgcagc agaaacgccg cgcacagacc ggcggcgatt ttatgcagca
     7021 ccacctccat caggttgtgg cggtttcctg ctgtatgcgc tgggggcagg acaaggttca
     7081 tgtcggcacc atcggcgaga tggacgatgg cgaagaagtc gtgattgcca agtttttcga
     7141 attggttgaa aaacatacgc cgcaactggt cagctggaac ggcggcggtt tcgacctgcc
     7201 cgtactgcat taccgctccc tgatatacgg catcaacgcc gcgcgctatt gggatacggg
     7261 cgacggcgat ttcggcgaca gccgcgattt caagtggaac aactacatca gccgttatca
     7321 ccaacgccac tgcgatttga tggatttgct cgcgctttac cagccgaggg cgaatgtgcc
     7381 gctggacgat atggcgaaac tgtgcggctt tccgggcaag ctgggtatgg acggcagcaa
     7441 ggtgtgggag gcgttccacg cgggcaggct gaaggaaatc cgcaattatt gcgaaaccga
     7501 tgccgtgaat acgtatttga tgtatctgcg tttctgtctg gtcagcggca gattcgacgc
     7561 ggacgaatac gaaatggaaa tcaagcggat cagaaactat ctctccgccc aaacagaaga
     7621 caaaccgcat tgggcagaat ttgtccaagc gtggaaatag aaacctgctg tccgaaggcg
     7681 gtacgcttgt gccgccgttt cggtttcaga cggcgtatgg tggattaaat tcaaaccggt
     7741 acagccttgc ctctccttgc cttactatct gtactgtctg cggcttcgtc gccttgtcct
     7801 gatttttgtt aatccgcttt aaaagctgta tccgtgccgt ctgaagcctt tcccagtcaa
     7861 accaaacaat attgaaagaa aaaaatgagc aaaaccgtcc gttatctgaa agattaccaa
     7921 acgcctgcct accgcattct tgaaaccgaa ctgcatttcg atattgccga accgcaaacc
     7981 gtcgtgaagt cgcgtttgac ggtcgagccg cagagggcgg gcgagccgct ggtgttggac
     8041 ggttcggcaa aacttttgtc cgtcaaaatc aacggcgcgg cggcggatta tgtgttggaa
     8101 ggcgagacgc tgacgattgc agacgtaccg tccgaacgct ttaccgtcga agtggaaacc
     8161 gaaatcctgc cggcggaaaa caaatcgctg atggggctgt atgcttccgg cggcaatctg
     8221 tttacccagt gcgagccgga gggcttccgc aaaatcacgt tctacatcga ccgtccggat
     8281 gtgatgtcca agttcacgac caccatcgtc gcggacaaaa aacgctatcc cgttttgctt
     8341 tccaacggca acaaaatcga cggcggcgag ttttcagacg gccgccattg ggtgaaatgg
     8401 gaagacccgt ttgccaaacc gagttatctg tttgctttgg tcgcgggcga tttggcggta
     8461 acggaagacc gtttcaccac catgagcggc agaaacgtca aaatcgagtt ttacaccacc
     8521 gaagcggaca agcccaaggt cggctttgcc gtggaatcgt tgaaaaacgc gatgaagtgg
     8581 gacgaaacgc gcttcgggtt ggaatatgac ttggatattt tcatggtcgt cgccgtaggc
     8641 gatttcaata tgggcgcgat ggaaaacaag ggtttgaaca tttttaacac caagttcgtc
     8701 ctcgccgaca gccgcaccgc caccgatacc gatttcgaag gcattgaatc cgtggtcgga
     8761 cacgaatatt tccacaactg gacgggcaac cgcgtaacct gccgcgactg gttccagctt
     8821 tcgctgaagg aagggctgac cgtgttccgc gaccaagagt tttccggcga ccgcgccggc
     8881 cgcgccgtgc gccgcatcga gaacatccgc ctgctgcgcc agaaccagtt ccccgaagac
     8941 gcaggcccga ccgcccatcc ggtgcgcccc gtcagctatg aggagatgaa caatttctac
     9001 accatgaccg tttatgaaaa aggcgcggaa gtggtgcgga tgtatcatac cctgctcggc
     9061 gaagagggct tccaaaaagg catgaagcta tatttccaac gccacgacgg acaggcagtg
     9121 acctgcgacg atttccgcac ggcgatggcg gatgcgaacg gcatcaatct cgaccagttc
     9181 gccttgtggt acagccaggc gggcacgccc gttttggaag ccgaaggccg tctgaaaaac
     9241 aatgttttcg agttaaccat taaacaaacc gtgccgccca cgcccgatat ggcggacaaa
     9301 cagccgatga tgattcccgt caaagtcggg cttctgaacc gcaacggcga agcggcggca
     9361 ttcgattatc agggcaaacg cgcaaccgaa gccgtattgc tgctgaccga agccgaacag
     9421 atcttcctgc tcgaaggcgt aaccgaagcc gtcgttccct cgctgctgcg cgggttcagc
     9481 gcgccggtgc atctgaacta tccgtacagc gacgacgacc tgctgcttct gctcgcccat
     9541 gacagcgacg ccttcacgcg ctgggaagcc gcccaaacgc tctaccgtcg cgccgtcgcc
     9601 gccaaccttg ccgcactttc agacggcatc gggttgccga aacacgaaaa actgcttgcc
     9661 gccgtcgaaa aagtcatttc agacgacctc ttggacaacg ccttcaaagc cctgcttttg
     9721 ggcgtgccgt ccgaagccga actgtgggac ggcacggaaa acatcgaccc gctgcgctac
     9781 catcaggcgc gcgaagcctt gttggatacg cttgccgtcc gcttcctgcc gaaatggcac
     9841 gaattggacc gtcaggcggc gaagcaggaa aaccaaagtt acgaatacag ccccgaaacc
     9901 gccgactggc gcacgctgcg caacgtctgc cgcgccttcg tcctgcgcgc cgaccccgcg
     9961 cacatcgaaa ctgttgccga aaaatacggc gaaatggcgc aaaacatgac ccacgaatgg
    10021 ggcatcctgt ccgccgtcaa cggcaacgaa agcgatacgc gcaactgcct gctggcgcag
    10081 tttgccgaca agttttcaga cgacgcgctg gtgatggaca aatatttcgc ccttatcggc
    10141 tcaagccgcc gcagcgacac cctgcaacag gttcaaaccg ccttgcagca tccgaaattc
    10201 aatctcgaaa accccaacaa agcccgttcg ctcatcggca gcttcagccg caacgtcccg
    10261 cattttcacg cacaagacgg cagcggctac cgcttcatcg ccgacaaagt catcgaaatc
    10321 gaccgcttca acccgcaggt cgccgcccgc ctggtgcagg cgttcaacct ctgcaacaag
    10381 ctcgagccgc accgcaaaaa cttggtgaaa caagaattgc agtgcattcg ggcgcaggaa
    10441 ggattgtcga aagacgtggg cgaaatcgtc ggcaagattt tgggttgagg cagtcaaaca
//
LOCUS       DAXDZG010000072        10203 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXDZG010000072 DAXDZG010000000
VERSION     DAXDZG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.072"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..129)
                     /locus_tag="ACFB78_001703"
     CDS             complement(<1..129)
                     /locus_tag="ACFB78_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFC1269650.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAE"
     gene            374..1282
                     /gene="hslO"
                     /locus_tag="ACFB78_001704"
     CDS             374..1282
                     /gene="hslO"
                     /locus_tag="ACFB78_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC1269651.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="ACFB78_001705"
     CDS             complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="ACFB78_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC1269652.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAKDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="ACFB78_001706"
     CDS             complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="ACFB78_001706"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC1269653.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            4040..4351
                     /locus_tag="ACFB78_001707"
     CDS             4040..4351
                     /locus_tag="ACFB78_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC1269654.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            4425..5636
                     /locus_tag="ACFB78_001708"
     CDS             4425..5636
                     /locus_tag="ACFB78_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC1269655.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5707..9663)
                     /gene="purL"
                     /locus_tag="ACFB78_001709"
     CDS             complement(5707..9663)
                     /gene="purL"
                     /locus_tag="ACFB78_001709"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC1269656.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            9814..10152
                     /locus_tag="ACFB78_001710"
     CDS             9814..10152
                     /locus_tag="ACFB78_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC1269657.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
BASE COUNT         2258 a         2645 c         2969 g         2331 t
ORIGIN      
        1 ttcggcaaac tgtctgagaa tctgctcgcg gctgctgagc aggttggtca actcgtcggc
       61 aagggcgggg cggacggcaa acatcagcca caaaaccgcc caaactgccg gtttgaaaaa
      121 agaatccata tcggtgtttc cgcgcaggag gcatcgtgcc gccgtgcgat gttatgctgt
      181 atcagtcgaa aatatcttgt tgattgtata taaaaaaacg gctgtttggc aaaaaacggc
      241 ttgttagatt gagttaatat gttgattttt atataattta ttgagaattt gggaagcgca
      301 tccgccgtcc gccttgtttt cgcgccgacc gcaaccatat agccgccatc cgaacaatca
      361 gtcagagaaa atcatgaatc aaaccgccat caaccgcgcc gatgcgcgta cccgctttat
      421 cttcgacgat atgcccgtgc gcgggctgca cgtccgtttg gaaaacgtgt ggcagcacat
      481 tgtaaaacag aaaaactatc ccgccgccat ccgctgcgcc ttgggagagc tactggctgc
      541 cggcgttctg ctgtcgggca accttaaaaa cgaaggcacg ctgattgtgc aggttcaggg
      601 gcagggaaaa ctgaaaatgc tggttgcgga agcgacttcc gaccgtaccg tccgtgcaac
      661 cgcgcggtgg gacgaaaccg cagaaatagc cgatgacgaa agcctcggcg accttttggg
      721 cggcaacggc gtgttcgtat tgacgctgca acccaaagac ggcgaaccct ggcagggcgt
      781 agtgcctttg gaaggcggca gcatcgcgca aatgttggtg aactatatga aacgttccga
      841 acagcttgat acgcacatcg ccctgtctgc aagcgatgaa gcggcgggcg ggctgctggt
      901 gcagcgtctg cctgaagagg tgttggatga agaggcatgg gaacacgtca gtacgctggc
      961 gcgcacgttg acggcggagg agctggcaga attggacgcg caacacgttt tataccgcct
     1021 gttccacgaa acgccgccgc gcgtgttcga gccggaaacg tttgaatcct catgtacctg
     1081 ttctcggggc aaggtcagcg atatgctgtt gatgctgggc ggggaagaag tcggcggcgt
     1141 ggtggcggaa caaggcagca tcgaagtcga ttgcgacttc tgccacagca aatacgtgtt
     1201 tgatgaaacc gatgtcaacg cgctgttcgg ggaggatgtg gtcggcgttg ccaaagggct
     1261 gccccgacat accgtccaat aatcttgagc aacaaggatg aatggtcgaa tgccgtctga
     1321 agcagcttct gcttcagacg gcatttttgc gcgggtcggg acggcattta aagcaggaac
     1381 agggtggcga gactcaagaa aatgaggaag ccgccggagt cggtaacggc ggtaatcagc
     1441 accgagctgc ccagcgcggg atcgcgtccg aatttttcca ttaccacggg aatcaatacg
     1501 ccgaccgttg ccgccagcag gaggttgagc gtcatcgcgg caaccatgac caaaccgatg
     1561 ccgaggctgc cgtaaagcag ccacgaaacc gctcccatca ccgtccccca aataatgccg
     1621 ttgaccaagg cgacgccgac ttcttttttc aacagccttc ccgcctgcat atccgtcagc
     1681 tgccccatcg ccatcgcgcg gacaatcatg gtaatcgtct ggttgcccga gttaccgccg
     1741 atgccggcga cgatgggcat cagcgcggcg agtgcgacga ttttttcgat actgccttca
     1801 aacgcgccga tgacgcggct ggcgaggaag gcggtgcaga ggttgacggc gagccacatc
     1861 cagcggtttt tgacggaatc caagacgggg gcgaacaggt cttcctcttc ctgcaaacct
     1921 gccatgttca acatatccgc ttcggattct tcgcggatca cgtccaccat ctcgtcgatg
     1981 gtaatcctgc cgatgagctt tttgttttca tcaacgacgg gcgcggtaac caagtcgtag
     2041 cgttcaaacg cctgcgccgc ttcttccacg tcgtccttgg cgcggaaacg cacgacatct
     2101 ttcgccatca cgttttccac caaatcttcg ggatcggcga ccaaaagttt gcggatgggc
     2161 agcacgccct gcagcacgtc gttttcatcg acgacaaaaa tcttgtcggt atggtcgggc
     2221 aggctgtcga agcggcgcag ataacgcagc accacttcgc aggcgacatc ggcgcggatg
     2281 ctgaccaact cgaagtccat aatcgcaccg acttggttgt cttcatacga cattgccgcc
     2341 ttgacttggg cgcgctcttc ctcatcgcgc gtctgtaagg cttcataaac cacttggtgc
     2401 ggcaaatcgt ctgccagctc cgccaattcg tccgcatcca aatcatcgac cgccgccaac
     2461 aattcgtcct tgtccattga ctcgatcagc gtttctcgca ccgcgtcgga tacttccagc
     2521 aatacttcgc cgtcgtcttc gggtttgacc agaagccaaa cgatattgcg ttcgcgcggc
     2581 ggcagggact ccaagacgtc ggccacgtcg gcagggtgca actcggccaa gaggacggtc
     2641 agctcggtca gcttgtcgcg cagcggcgca tcttcgagcg gtgtgccgtt ttcgatttgt
     2701 tcaaaagcag gttcgaggat ttcgcagagg gagtggacac ggtcgaaatc ggcggaaggg
     2761 cgttcggcat ggttttgacc ggtttcgccg tcgtgtgtcg gaggagtgtg ttcgatgctc
     2821 ataaatgctc cgcccgccgt gtgcggggag gcattccggc gggatggtta ttgtcgtttg
     2881 gaatcggaag gggtgttcag taaaaacgaa ctgggaaggt ccataataaa agcccgaaaa
     2941 atcaggcacg ggattggaaa cagcactatt ctactccctt ttgaaacggt ttactatttt
     3001 taacgcaaat gccgtctgaa acgcgggttt cagacggcat ttttcggagg gcaggtcagc
     3061 ggtaggcgtt tttcagttcg cgcagggcgg cgaatacttc caaaccgctg ttcagcgttt
     3121 tgccgaccag ggcttcggca cgttggcgga cggcggggat ttctgtccgt aagaacgggt
     3181 tgacgcggcg ttcgtgcgcg agggtaacgg gcagggtggg cgtgcgttcc gccgccttca
     3241 atgccgtctg aatgtcggcg ttgtccggct cgatatgggc ggcgaaacgc aggttggcgg
     3301 cggtgtattc gtgtgccgga tagaacaggg tgccttcggg taattggttg aaccgttgga
     3361 agctgtcgta aagctgttcg accgtgccgg taaacacgcg tccgcagccg gcggaaaaaa
     3421 gggtgtcgcc gcagaaaacg tgtatgccgt ctgaagtttc gagaaggtag ctggtgtggc
     3481 ggtctgtgtg gccgggtgtt gcccaaacgg taacctgacc gttgccgaag gtgaatcgtg
     3541 tgccggcggt tacggtgtgg gtggcggctt cgatgtcgga ttcgccgtaa acgggcgatt
     3601 ccatgtagcc gcgccagagt gccgccgcac cgccttcgtg gtcgggatgg gggtgcgtta
     3661 cccaggtttg ggcgagcatg agtcggttgc gtacgaggaa ttccaagacg ggcgagggtt
     3721 cggaaggatc gacgcagacg gcatggttgc cgtgctgtat catccagatg tagttgtcgg
     3781 tcagggcttt gacgggggta attttcatga gtgttccttg atggttcgga tgccgtctga
     3841 aaggtgtatc cgaaaggcgt taatatacag cggaacccta gcaaacgcca tcatgctgtt
     3901 tcaggcggca ttttttctta taaaaacctt tcagcaacaa tatattgcca aactgcaaac
     3961 caaactgcct ttgataactg ccgaccttcc ctctaaaata cgttcgaatt tttaaatata
     4021 taaactcaat agatcgaata tgccgcaaca aaccatgaat ttaatgcgcg agtgcatacc
     4081 catttttacc gtattgagcg atgaaaaccg ccatcagatc ctgcatgtat tgtggaaaca
     4141 cggtaggatg aatgtgaatg agctgaccga gcatctgcat ctgtcgcgcc ctgctgtgtc
     4201 gcaccattta aaaatcatgc tgcaagccgg agcggtggcg gtggagcagg tcggcaaaga
     4261 gcggttttac agtattgcca tggcggatgc ggtggcgaga ttgaaacagc ttgccgattt
     4321 gatggctcaa aattgcccgc tttcaaaata gggttatccg ttggcccttt atttttaccc
     4381 aaatacgttt acatttttaa acttatcaac ttaaaggaac gatcatgtta ttcacgccat
     4441 tcacttttca aaacggcaaa accgccaaaa accgtatctt caaatccgcc atggaagagc
     4501 aacttgccca aaacgaccag ccgtctgaaa aacttgtccg cctgtacggc gcttgggcag
     4561 aaggcggcgc aggcgttttg gtgacgggca atgtgatggt ggctgaaagc ggcaaaggtt
     4621 cgataaacga tgtgttgatt tcagacgacc gcgcccttga aatgctgaaa aaatgggcga
     4681 aagcccgcat gcaaaacgac acgctgctca tcatgcaaat caatcatgcg ggcaaacagt
     4741 cgcctgcggt ggtcaataaa acaccgcttg caccgagcgc cgtgccgctg gtgggcatga
     4801 atggctttat caatccgccg cgcgaattga gtgccgatga aatcaacggg ctgattcagc
     4861 aattcgtaca aactgccaaa attgccgaac aggcaggctt ttcaggcgta caaatttacg
     4921 ccgtacacgg ttatctcatc agccaattcc tctcgccgca ccacaaccgc cgccaagacc
     4981 aatggggcgg cagtttggaa aaccgtatgc gcttcctttt ggaaacctac accgctattc
     5041 gcgctgccgc gggcaaagat ttcttggtgg gcgtaaaact caattcggca gatttccaaa
     5101 aaggcggatt tgacgaaagc gaatcggtgc aggtggtgca aaaactgtcg gaaatgggca
     5161 ttgattttat tgaagtttcc ggtggcaact acgaaagccc gcaaatgctc gccgccaaag
     5221 acagcacccg caaacgcgaa gccttcttca tcgattacgc cgaaaaagcc cgtgcagcca
     5281 gccaagcccc gctgattatc accggcggat tccgttcgca aaccgccatg gaagatgcct
     5341 tatccagcgg tcatttggat ttggtcggca tagcccgccc gtttgcctta gtccctgatt
     5401 tggcgaacaa aatgcaaaac agaacttacc aaaccgtaca agcagaccgc atccaaactg
     5461 gcgtagcatt tgttgataaa aaagcgggtg caatgttaga aatgaactgg tatatgacgc
     5521 aaatggattt gatcggacaa ggaaaacagt ccaaccctaa attgtcggcg tggaaagtat
     5581 tgctgaaaac tttgcgggaa aacggtaaag cgggattgag taccggcagg gcttgagaat
     5641 ggtcaaaatg ccgtctgaaa cgggtttcaa aacctttttc agacggcatt tgtttgggca
     5701 gacggtttag cccaaggctt tacgtgcgcc ggcaaagagg cggtaccagc cggacagttc
     5761 cgtccagcct tccggtttcc ggctcatttg tgcggcacgg tacacgcgtt cggggtgggg
     5821 catcataatg gtaacgcggc cgtcggcgtt ggtaatgccg gcgatgccct gcggcgagcc
     5881 gttggggttg agcggatagg tttgggtaac ctggttttga ccgtccacat attgcagcgc
     5941 gatgcccaaa tcggcggaaa tattgccgcc gtgaagcgcg aagtcggcgc ggccttcgcc
     6001 gtggctgacc acgacaggca ggctggagtc ttgcatttcg ttcaaaatca gcgacgctga
     6061 tttggggacg tgaaccatat tcaggcgcgc ttcaaactgc tcgctcaggt tgcgtttgaa
     6121 cttcggccat gtttctgcgc ccgggataat ttcggcaagg ttgctgacca tttggcagcc
     6181 gttgcacacg cccaatgtca gcgtgttcgg atcggcaaag aaggcggcga attggtcgcg
     6241 caaggcaggg tggaacagga tggatttcgc ccagccttca cccgcgccga gtacgtcgcc
     6301 gtagctgaag ccgccgcacg ccgccagcat tttgaagtcg gcaaggcgga agcggccagc
     6361 catcaggtcg gacatatgca cgtcgtaggc atcgaatccg gcgcgggtga aggcggcggc
     6421 catttcgatt tgcccgttta cgccctgttc gcgcaggatg gcgattttag gtttcgcgcc
     6481 actgttgata aacggcgcgg cgatgtcttc gttcacgtcg aacttcacgt cggcaaacaa
     6541 tgcgctgcgt tcgttgtcgc caatcagggc gaactcgctg tcggcgcagg cagggttgtc
     6601 gcgcaggcgt tggatggcgt ggctggtttc ctgccacgcg cgttgcaggt cggcgcggtt
     6661 ttgttccaag aaataaccgt aaccaaagaa agaaataaac tcattgtcaa aatcaggaga
     6721 gccaatccgg ctgaccgcat cggttaagcc ggccgtttca aatgccgcat caacataatc
     6781 cctatctttt tggcggattt ggataaccgc gcctaattct tcattgaata agattttaat
     6841 ggcggcatga ttataaagtt cgtcagataa gtcttcggca gggtcgcctt gaaaatccgg
     6901 gagatgaatc ggcaggaatt tatccatcag gcaatctata tcggcactaa taccgcaccg
     6961 tgccgcaaac gccatttccg ccagcgtcgc aaacaaaccg ccgtcgctgc ggtcgtgata
     7021 cgccaaaagt ttgtcttcgg cgacaagctg ttgaattacg ctgtaaaacg ctttcagacg
     7081 gcctgcatcg tccaaatcgg gcgcgtcgcc gctcatgttg ttgtacacct gaccgaacgc
     7141 cgagccgccc atgcgcgctt tgccgaagcc caaatcgata aacagcaata cgctgccttc
     7201 gacgtttttc aattcgggcg taacggtttt tcgcacgtct ttaacaggcg cgaacgcgga
     7261 aataatcagg ctcaacggcg aaaccacgga ttttttctct tcgccgtcct gccacacggt
     7321 tttcatcgac aggctgtctt tacctacggg aatgctcaaa tccaatgcct ggcaggcttt
     7381 agaaacggct tcgacggtgc ggtagagttt ttcgtcttcg ccttcgttgc cgcacgccgc
     7441 catccagttg gcggaaagtt tgatgttgcc gatgtcgccg atgttgactg ccgcgatgtt
     7501 ggtgatggct tcgccgacgc acattctgcc cgaggcgggc gcgtcaaaca gggcgacggc
     7561 cggtttttcg cccatagaca tcgcttcgcc gcgataggtg ttgaagccca tcatggtaac
     7621 ggcgcagtcg gctacggggg tttggtattt gccgaccatt tggtcgcggt gggtcatgcc
     7681 gccgacgctg cggtcgccga tggtaatcag gaagtttttg gcggctacgg caggcaggcg
     7741 caaaacgcgg taggcggctt cggtgatgtc gatatcgccc gcgtgaaacg gttttttgga
     7801 cggcgtaacc gttttgtcgg tacgcgtggt tttgggcggt ttgccgagca agacgttcag
     7861 cggcaaatcg acggggttgt tggagaacaa atcgtcgcgc actttcaaat gaccgtcgtc
     7921 ggtcgccgtg ccgactacgg caaacgggca gcgttcgcgt tcgcagatgg cgcggaaggt
     7981 gtccaaatct ttttccaaaa tcgacaagac ataacgctct tgcgattcgt tacaccagat
     8041 ttgcaaaggc gttaaaccgt gttcttccaa cggcacttcg cgcagctcga ataccgcgcc
     8101 gcgtccggca tcgttgacca attcagggaa agcgttggac aagccgcccg cgcccacgtc
     8161 gtggatggag ataatcgggt tttggtcgcc gagctgccag cagcggtcga tgacttcctg
     8221 cgcgcggcgt tcgatttcag ggttgccgcg ttgtacggag ttgaagtcca aagacgcatc
     8281 atttgtaccg gtatccatcg aagaagccgc gccgccgccc aagccgatga gcatgcccgg
     8341 gccgcccagt tggatcagca gtgcgccttc ggggatttcg tctttgtgcg tctgctgcgc
     8401 ctgaatgctg cccaaaccgc cggcaatcat aatcggtttg tggtagccgc gaacctgacc
     8461 gtcaaatttt tcttcaaagg tgcggaagta gcccaagagg ttcgggcggc cgaattcgtt
     8521 gttgaacgcc gcaccgccaa tcgggccttc aatcatgatg tccaacggcg aggaaatgtg
     8581 ttccggcttg ccgtagtctt gttcccacgg ctgtttgagg tcggggatgt tgaggttgga
     8641 cacggtaaag ccggtcaagc ccgctttcgg acgcgaacct ttgcccgtcg caccttcgtc
     8701 gcggatttcg ccgcccgcgc ctgttgccgc gcccgcaaac ggcgcgatgg cggtcgggtg
     8761 gttgtgcgtt tccactttca tgatgatgtg ggtgtcttcc tcgtggaaac ggtagccttg
     8821 gttttccgct gcattcggat agaaacgctc gattttcgcg cctttgatca cggacgaatt
     8881 gtctttatag gcgacgaccg taccttcggg atgcgcgttg tgcgtgtcgc gtatcatgcc
     8941 gaacagggat ttcggttgtt tttcgccgtt gaggatgaaa tcggcgttga agattttgtg
     9001 gcggcagtgt tcgctgtttg cctgcgcgaa catcatcagc tccacatcgg acggattgcg
     9061 ctgcaaagcc tgatagtttt caaccagata atcgatttcg tcggcggaga gtgccaaacc
     9121 cgtttcggta ttggctttga ccaaagcctc tttaccgccg cccaaaacat cgacggtgga
     9181 gaaggtttcg gatttgagat ggtggaataa tttggatgcc gtctgaaaat cgggcagcac
     9241 ggattcggtc atgcggtcgt gcagcaacgc cgcccattgc tgtttctgtt catcggtaag
     9301 cgcaccttcc agccacaccg ccataccgcg ctcgatgcgt tcgatgcctg ccaaaccgca
     9361 gttttccgcg atattggtcg ccttggaagc ccacggcgaa atcgtaccca aacggggcgt
     9421 taccaaaaac aaatgcaaac cctcgcgcgc ttcgggcgtt tgttcaacgc tttgcgccgc
     9481 caacaaggct tgcagttttt cgacagtcgc ggcatcaagt gctttctcgc tgccggcgaa
     9541 ataccaaaat tcgctgctta atttgacttc gggcaggccg agtgcggcgg ctttttgcaa
     9601 gagtttttcg acacggaaat cggaaagggc ggtaacgccg cgcaagggca aaacaacaga
     9661 catggattcg gctctcaaat gcggttggaa aagctgtcat tatatgcgca acgggggctg
     9721 tattgcttgg gtattttggt ttaaaatgtt gacaatctga cgggggttcc cgtttttcgg
     9781 atgtgtttgt attgtttgtt caaaggaaat tttatgaaaa aaatcgaggc gattgtcaaa
     9841 ccgttcaagc tcgacgacgt gcgcgaggcg ttgacggaaa tcggcattac gggcatgacc
     9901 gtcagcgagg tcaaagggtt cggcaggcag aaggggcata cggaaatcta tcgcggtgcg
     9961 gaatacgccg tcgatttcct gcccaaggtc aaaatcgagt tggtgttggc ggatgatgcc
    10021 gtggaacgcg cgattgacgt gattgtcgag gtggcgcgtt cgggcaaaat cggcgacggc
    10081 aagatttttg tgctgccggt cgaggaggca atccgtatcc gcacgggcga acgttcggac
    10141 gcggcggttt gagcgtttgg aatggaaatg ccgtctgaaa ggctttcaga cggcatttgt
    10201 ttt
//
LOCUS       DAXDZG010000073        10074 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXDZG010000073 DAXDZG010000000
VERSION     DAXDZG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.073"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(341..3173)
                     /gene="lbpA"
                     /locus_tag="ACFB78_001711"
                     /gene_synonym="tbpA"
                     /pseudo
     CDS             complement(341..3173)
                     /gene="lbpA"
                     /locus_tag="ACFB78_001711"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
     gene            complement(3170..5352)
                     /locus_tag="ACFB78_001712"
                     /pseudo
     CDS             complement(3170..5352)
                     /locus_tag="ACFB78_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(5505..6428)
                     /gene="era"
                     /locus_tag="ACFB78_001713"
     CDS             complement(5505..6428)
                     /gene="era"
                     /locus_tag="ACFB78_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC1269658.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(6475..7194)
                     /gene="rnc"
                     /locus_tag="ACFB78_001714"
     CDS             complement(6475..7194)
                     /gene="rnc"
                     /locus_tag="ACFB78_001714"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC1269659.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSSGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            7361..7684
                     /locus_tag="ACFB78_001715"
     CDS             7361..7684
                     /locus_tag="ACFB78_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC1269660.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            7731..8207
                     /gene="ribH"
                     /locus_tag="ACFB78_001716"
     CDS             7731..8207
                     /gene="ribH"
                     /locus_tag="ACFB78_001716"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC1269661.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            8285..8710
                     /gene="nusB"
                     /locus_tag="ACFB78_001717"
     CDS             8285..8710
                     /gene="nusB"
                     /locus_tag="ACFB78_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC1269662.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(8789..9823)
                     /gene="pyrC"
                     /locus_tag="ACFB78_001718"
     CDS             complement(8789..9823)
                     /gene="pyrC"
                     /locus_tag="ACFB78_001718"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC1269663.1"
                     /translation="MQTLTIIRPDDMHLHLRDDDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(9884..>10074)
                     /locus_tag="ACFB78_001719"
     CDS             complement(9884..>10074)
                     /locus_tag="ACFB78_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269664.1"
                     /translation="LRAEQSVQAPFETRQISYAWRESGSPEETVLTAQTADSPDLPVQ
                     AVLNLTHRSHNGKPLILP"
BASE COUNT         2217 a         2879 c         2428 g         2550 t
ORIGIN      
        1 tctcaaaaat ttatgccatt aaatcaataa tttctgatga attttgaaaa cttaatcccg
       61 tcattcccgc gcaggcggga acccggttca ttgagcttca gctatttaga ataaattttg
      121 aaactctaat cgcgtcattc ccacgaaagt gggaatccgg ttttttgagt ttcagtcatt
      181 tccgataaat tgccttagca ttgaatgtcc ggattcccgc ctgcgcggga atgacggcgg
      241 aaatcttgtt tatattgaat caaaaaaacc tgcaccttaa tcagttggcg gtttagtccg
      301 acttttgggg tgcagatcaa gcttccagac ggcatttcct ttaaaacttc atttcgagcg
      361 cgaggctgaa gttcctgccc ggtgcggcat accttccata gttgctgtcg ccgccgtgcc
      421 ggtttgccgt gctttccgca gtctggcgca aggattccca agtaacgtag cggtagttgc
      481 cgatattgta gatagccgcc ctcaaggtca gccgtttttt cagattcaga taagcggaaa
      541 catctgccgt tttccaagaa gacgtaaccc ttcctgccga atatcgtttt tgatcgcctg
      601 ccagataagc aagctcgtca gggtttttcc ctttggaata ggtcagcata atgtttgcgc
      661 cccatttccc ctcgggctgg tcgtatccga accccaaaac ataacgcgac ggctgtaccg
      721 catccaaagc atagctgcgg agggacaagt ccggccggtt ggataccgat ttcggtttga
      781 tacggttgta cgccaatgtg gtgtacaggc cttcgggcag tttgccgtat acgccgttcc
      841 agtcgatttt ccccaagatg ttgatgcctt gaagcgacat attttgggca ttgtaataat
      901 cgcgtatatc aatctctgtc aatcgtcctg ctgaatccgg caatttggtt ttttgatcgg
      961 caacggcaat catatcggta taacggttgc ggaaactgct gatttccaaa aagccgaaat
     1021 cgcccttcca ctgcaaaccg atttcccggt tggctgcctt ttccgatttc agggcgggac
     1081 gctgccagcc tttcggataa tcgtgataaa tgtctatccc gaaaagttct tggaaggaag
     1141 gcgttctgaa gccgctggag gcgcggtaag acacggaaaa atgccggttc ggtttgaaca
     1201 cgatgccgct gttccacgaa cggtcggcat accgcccgct gcggacgagt tcttccgacg
     1261 tggtgaagtt ttgccggtca tacctgccgc ccaagctgaa atcaaaatat ttgccgattg
     1321 aaaaacggtc gttcaaagaa atgtggatat tgctgccgtt gatttttctt ggcacgcatt
     1381 tgcgggaacg cagggtttcg atgtagccgc agaccgaccc ttcgacgact tcgggcttac
     1441 ccaaacggta cttatcttga ttcttttcat cgaatcccgt ggattccgaa atccttgccg
     1501 cattgtggga aagctgttcg gggcgggaga tcgctttgga agcatcgtaa ccgaagccca
     1561 aagtcagatg gtgtttcgtc catttgtttt tcagcgactt ctcaaacgag gcattcaaaa
     1621 cattgtgctg ttcgcggtag tggaaacggt cgctgctgtc gtaggaatac ggtttgtccg
     1681 ccgacgcgcg gcaggatttg tccacggcag gatacacggc gcaattcagc ttcagcgtgt
     1741 tgttgtcggt tgccacgccc tgtttgtcaa acgacaacac cgccttatcc gcccagttgt
     1801 ccgagtattt ctcattttca taacggtaca gcaaacccat acggcggcgg cggtgatgtt
     1861 cgtcaataaa tttggtgcgg gaatatttca aacctatgcc cttgaccaaa tttttatcgc
     1921 ccttccactc ttctatattc ggcacaaaat acaagccgtc gcggaaatcg tcgccgtcgt
     1981 acaccccgct cttgtctcta aacttttccg cctcgtccgt accgtaatac tgtttttccg
     2041 tcatatcgcg gatgtcgtaa cgctgtttgg tatcctcaaa cacgccgccg acataatgcc
     2101 tgccgccgaa gcggtagccc agcttggcaa gccaagagcc gctgcggtaa tccatcggat
     2161 cgggcaatat cctgccgccg cccgtgtagg cttgggcgga caggttttcg tggcgcgcct
     2221 gcgcctcccg cacctgcgcc tcttcttcag cacttaaaag gctgattttg ttcaatacgt
     2281 tcttttaccc agcggttgag ctggcggttc aaatatatcc cgtagcccgc caattttgcc
     2341 acgggcttga gtgtattctc gccctctact aagaaaaatg gctctgttgt cttgcgttta
     2401 atatcgtatg tctggcggaa cgcgtccaaa cggtctatgc cgtattccac cccgtccgca
     2461 atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg ttcggattag cagcccttcc
     2521 caaccgtctt tgctgaaccc cgcgccgagc gacttcataa attggcggtt tttactgccg
     2581 taagcggttt ttgcctgtat cccccggctt ttgccgtctg aaatcaggtc tgccgcctct
     2641 ttggtgcgga aggcgaccgc gccgccgagt gcgccgctgc cgtgatcgga cgaaccggcg
     2701 cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat tgcgccgctg
     2761 ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg tatttgggca
     2821 acgccgtcaa ccgaaaccgc cacacggttt ttatctacgc cgcgtatcga gtagccgccg
     2881 ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt caggtcgcgg
     2941 ataccgagta cctgttcttt gttcaacgtt tccgacgttt tgacgatttt gcccaaacct
     3001 gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc tttcaatgat
     3061 tgactttgcg ccgcatcaag tgccgccgcc cccgcttggg cagcataagc cggaaaagcg
     3121 gttgcaatgg ccaacgccgt caaagtcagc ggaaaaccgt gtttcttatt catttttcca
     3181 cctctttatt atctttcttc gcaccgaata ccacgccgaa ttggggttta acttcaggtt
     3241 ctaactgttc gccaacatca gcttcatttt caacttcatt ttcaatatct tcagttatac
     3301 caagagattt cccatcctta ttgaaaatag tgccgcccaa ttctgccgcc tgcgggccgt
     3361 aaaagccgcc cgttacaaga agattgtcgg ctttgaaact ttgaggatta gtcgaaccat
     3421 ttcccgaaag attgatgccg ttatcccgag tgcgtgctgt tgcgtggaaa ccgttgccct
     3481 caatcacgcc gttttcaata cggaaagcag gttctacacc gttttgctcc gtcagcgttc
     3541 cggaaatcga tttattgccg aaatcaacgt caaattctgc ttttgccgct ttatccgcct
     3601 tattatccca ttgaatgggt tcgccgatat gcgcttccca agtgccggta taatgcgccg
     3661 ttccggtttt tggaatgtcg gcttccgccg tgcggatacc tttcaggaaa aggtcgatgt
     3721 ccctgccttt aagggcttcc ggggcgggcg ggatgccgtc tgaaacaccg ttgccttctt
     3781 ccggcgattc ttcttcgggt tcttcctctt cagcttcttc ttcgatttct tcagcttcat
     3841 cagcttcttc ttcgacgatt tcgtcttcgt cttcgccgtt atcatcttcg gaaacttcgt
     3901 cttcgctttc ttcggcgatt tcttcgtctt cgctttcttc accgttatta atacccgaat
     3961 cctcttcgtc ctgcgccttc ggtttgacgg cggggcgttc ggtttttatc cgtccgagtt
     4021 tcacataggt caaaaagtcg caacaagcac tgacggtcat tttcctgccg tcggcaagat
     4081 cgatggtttt ctctttgcta accaaaggaa tttcacgccc ttcgacaaga agtttgtcag
     4141 gatgaccaaa atcgggcata gtggaaacct caaacggacg gggattttca tcagtcgcct
     4201 cgtcaacgga aatttttaga gaatccaaga ttttggtgtg ttttccaaac ggcatagcag
     4261 gatttgtgcc tgatgcgttt gttttttgtt tgcctgcgaa tacgccgaat acgccgttgt
     4321 cgttactgat aaaccgtccg gcaagctctt ctccgttatc gccgaaaaaa ccgccctcaa
     4381 gccgctgatc ggcatcggta tggaaaaaca aatattcttt atcagcgtgt ctcgtcttca
     4441 cctcggtgtt aactttggca ctgccggtaa agcggttgcc gtttaaatcg gcagtgatgt
     4501 tgtaaatggt cagcggtttt ttaggatcat ttttcttttg cacatactga tttttaatca
     4561 gctcgccctt taggattttt ttgtcgaaat caaccgtata ttcggcagga tgtttttccc
     4621 tgtcgtcggc atccttagcc gcataagaag ttgcaccaat atcgttacca taaaatgtta
     4681 tataacccaa atcgtcactg gcaaccgcct tacctgtccg atgacgtttg acatcggtca
     4741 tatattgcca gttgccggaa tattgcaccg ttcccgcgct cggtaaagat tgggaaggac
     4801 gttctccgga ataatataca aaaccgtcat aaccaaaacg gtgggtaaac cgcttaccac
     4861 ctgagttctg ttcgatttca tcttttccgt ttttagtata tacataaccg gcatccacaa
     4921 atttataatt atatttttta ttttcctttt ctgacgtcct aatttctaca tcggaatcac
     4981 gtttatgaat ttcgctttta agttgttgaa gtttatttcc ttttgaaccg tataaaaaca
     5041 gaacatcacc ttctttaaac gacagctttt cttctgcttg tttgctatcg ggaattgccg
     5101 tgccatcttc acgatgaaaa gcagtattcc gcctcaacaa ccgcattgcc gcaccgacgg
     5161 cgggccggtt gaccggcgtg gtttctaccg aaggctcggc aggggggggg aagtgggaac
     5221 gtccttagac ttgaaagtga cggggtacgc ggtcggcgtt gattcgacaa caggctgcac
     5281 gccgaaattg ccgccgcagg atgccagcat caacggcagc aaggcaatac cgtaataatt
     5341 cagtttacgc atccctatcc cccctattta attaataata attatcatta tattaatatg
     5401 cacagataat atcaacccgt ttttaacaaa gatattcccg attgaaacaa aatgccgtcc
     5461 gaaacctgtt cagacggcat ttgcgtaaag ttcagcaaga aaaactacaa acccagctcg
     5521 cgcaggaagc gaatgtcgtc tgcccaaccg gatttgactt tgacccagac cttcaaaaat
     5581 actttgttat caaacagttt ttccatatcc agccgcgctt cggtggaaat ttttttcaaa
     5641 cgctccccgc ctttaccgat caaaatcgcc ttttggcttt ctttgtcgac caaaacggcg
     5701 atgtagatgc ggttcaaacc gtctccctct tcaaactgct ccacttcgac gttcatcgca
     5761 taaggcagct cctcgcccaa atagcggaag agtttttcac gcacgatttc catcgccaaa
     5821 aaacgcgccg atttgtccgt aaccatgtct tcgggataca tcggtacgct ttcgggcaga
     5881 tacggcttga gcagctccaa caggttggca atccgcaaac cgtgtttcgc actgaccgcc
     5941 tccgccgccg caaattcaaa ttcggcgcgc acttgggcaa caaacgcctc cagcgcgtaa
     6001 cggtctttcg ccttgtcctt gtcgattttg ttgatcacta aaatgaccgg cgtgtgcttg
     6061 ggcagttgtt tcaacacgac gcggtcggca tcggtaaggc gcatcgcctc cacgacgaaa
     6121 accaccacat ccacaccgcc gagcgcctcg gtaacatttt gattcagcct gtcgttgagc
     6181 gcgttgcggt ggtcggtttg aaagcccggc gtatcgacaa acacgaactg cgcggtatcg
     6241 tcggtataaa tccccgttac gcggttgcgc gtcgtctgcg cctttttgct ggtaatactg
     6301 attttctgac cgatgagatg gttcatcagc gttgatttgc ccacgttcgg acgaccgaca
     6361 atcgccacga agccgcaacg gtatccgccg gcggcgcgtt cccctgcaag gaaggtttca
     6421 atatccatat tcgctttcat atccacattt cagacggcat attcacgcgc cgcctcattt
     6481 ctttttcttc ttcagcggca gcttctcttc cagccatttc aaagcctctt tcgccgcctc
     6541 ctgttccgcc gccttgcggc tcgtcccttt ggcacggcac acgaaaccca gttcgcccaa
     6601 atcgcaggaa atgacaaaca tactgtcgtt ggcatggccg atttgctctt cgatgcggta
     6661 tttcggcaag gcgaaacggc gcgcctgcaa cgcctcctgc aaagcagttt tgccgtcttt
     6721 tgcctgattt tggaaatcgg cgcgccggac gcgttctgca aacaaatggc gcaccacctt
     6781 ttccgccgtg ttgaaatcgg catcgaagct gacggcggca aacatcgcct ccatcgcgtc
     6841 cgccagtatc gaaggccgtc tgaagccgct gctcttcaac tcgcccgccc ccaaatacag
     6901 accgtcgccg acattcattt ccgccgccat ttccgccagc acgccctcat tgaccagact
     6961 cgccctcaac cgcgacaact cgccctcggt caacttcgga aacgcgtcaa acagcatccg
     7021 cgccaccgta taattcaaaa tcgaatcgcc gacaaactcg aaccgttcgt tgtgcttcgc
     7081 atgatggctc ctgtgggtca aagcccgccg caaaagcgac atatcgcgga acgcgtagcc
     7141 taactttttc tgtatcgcag tatgtgcctg ccgtttcaaa acatcgtctt tcattaccgt
     7201 cttccgcaaa aaacagcccg aatgcgtttc catactccaa aaatacagaa acaccttaag
     7261 gctgcaaacg gcgcacattg taccaccaaa ccgccgccgt ctgccgacaa cgcgggaatc
     7321 gtggtaaaat ccgcaagttc ccccccaacg tattcccgca atgaacgcat ttgcacaagc
     7381 actttcctcg gcactcgacc gctgcatcgc caccaacacc gtcgccaaac aaaaccggcc
     7441 cgtcggcttc ctttaccgcg aagcccccgt ttttgaaaac gacagcggct ggcgtttctt
     7501 cagcggcgac gaaaccgacg aatacaccga cgaccccgac aacttcagca tcgtcagcct
     7561 cgccgacatc accaaaacca accctgaaac cgccgcactg ctttcccaac ccgaaggcag
     7621 cgcgtgggag cttgccgaag acggcacatt ccaaaccgtt gccgactggc agccgcaaga
     7681 ctgaaacctt cagacggcat tccgaaaccc cgaataccaa aggacaccca atgaacacca
     7741 tcgcccccaa cctcgacggc aaacacctcc gcatcggcat cgtacaggca cgcttcacca
     7801 acgaaatcgg cagccaaatg ctcaaagtct gctgccgcac cctccaagaa ttgggcgtag
     7861 cagacgaaaa catcaccgtc gccaccgtac ccggcgcgct tgaaatcccc atcgcgctga
     7921 tgaactttgc ctcttccgaa aaatttgacg cactgattgc catcggcgtc gtcatccgtg
     7981 gcgaaaccta ccatttcgag ctggttgcca acgaatccgg cgcagggatc ggccgcgtcg
     8041 cactcgacta caacatcccg attgccaacg ccgtcctgac caccgaaaac gacgcgcagg
     8101 caattgaacg gattggagaa aaagcctcgg atgccgccaa agtcgccgta gaatgcgcca
     8161 acctcgtcaa ccttctgctc gaagaacagt ttgaagacga agaataacaa acagggcgta
     8221 caccgatgcg ccctttccgt ttcagacggc ataccgtgcc gcccaaccgt ttcaaggaaa
     8281 aatcatgaaa acagcccgcc gccgttcccg cgagcttgcc gtacaagccg tttaccaatc
     8341 ccttatcaac cgcaccgccg cgcccgaaat tgctaaaaac atccgcgaaa tgtccgactt
     8401 tgccaaagcg gacgaagaat tgttcaacaa actcttcttc ggcacacaaa ccaacgcagc
     8461 ggactacatc caaaaaatcc gcccgctgct cgacagggac gaaaaagacc tcaaccccat
     8521 cgaacgcgcc gttttgctga ccgcctgcca cgagctttcc gctatgcccg aaacgcccta
     8581 ccccgtcatt atcaacgaag ccatcgaagt taccaaaacc ttcggcggca cggacgggca
     8641 caaattcgtc aacggcatcc tcgacaaact cgccgcccaa atccgcccag acgagcccaa
     8701 acgccgttga tactcggaaa atccgaatcg gaaaatacac aaatgccgtc tgaaaacctt
     8761 tcagacggca ttttgcttga ccgttcaatc aatactgcac cgtccagccg atttcgccgc
     8821 ccgcgcgcat cgggacaagt tcgccgtcgc cgtaagggac gcttgcggga acggtttggc
     8881 tttgtttgac aagggtaatc gtgtcggcgt tttccggaat gccgtagaac cttgcgccgt
     8941 tttccgaggc gaaggcttcg agtttgtcca acgcgcctgc tttttcaaac acttcggcgt
     9001 aaagttcgac ggcggtcatc gcgctgaaca taccggcgca gccgcaggcg ttttctttgg
     9061 cggacttggc gtgcggcgcg gagtcggtgc cgaggaagaa tttgtgcgcc ttctcgccgg
     9121 taacggcggc gaccaatgcc tgacggtggg tttcgcgctt gaggacgggc aggcagaaat
     9181 gatgggggcg cacgccgccg accaagaggt cgttgcggtt gagcaggagg tgttgcgggg
     9241 tcacggaggc ggcaacgttg tcgcccgctt ccaaaacgag gcgggcggct tcggcggtgg
     9301 tgatgtgttc gaacacgact ttgagattcg gcacttgcgc caaaacgggt ttcatcacgc
     9361 gcccgataaa ggcggcttcg cggtcgaaaa tgtcgatttc ggggtcggtt acttcgccgt
     9421 gaacgaggaa caggatgccc tgttttgcca tttcttccaa cacggggatg agcttgaaca
     9481 ggtcggttac gccggaatcg gaattggtgg tcgcgcctgc ggggtagagt ttgaaggcga
     9541 cgatgccggc ggctttggct tcgcgcacga gttcgggcgt ggcttggtcg gtcaggtaaa
     9601 gcgtcatcag cggctcaaac gcgctgcctt cgggcaacgc cgccataatg cgcgctttgt
     9661 aggcaagcgc gtcggctaca ctgacgactg gcggtttgag gttgggcatg atgacggcgc
     9721 gccccatctg gcgggcggta taaggggcaa cggctttgag cgcgtcatcg tcgcgcaggt
     9781 gcaggtgcat atcgtcgggt cggatgatgg tcagggtttg catggttgtt cctttttaga
     9841 tttttggttt ccagacggca tgccgacttt atgccgtctg aagtcagggg agaattaggg
     9901 gtttcccgtt atgggaacgg tgcgttaggt tcaatactgc ctgtacgggt aaatccgggg
     9961 aatcggcggt ttgtgcggtc aatacggttt cttcagggct gcccgattcc cgccaggcat
    10021 agctgatttg cctggtttca aacggtgcct gcaccgactg ttccgccctc aatt
//
LOCUS       DAXDZG010000074         9914 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXDZG010000074 DAXDZG010000000
VERSION     DAXDZG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.074"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            446..1009
                     /locus_tag="ACFB78_001720"
     CDS             446..1009
                     /locus_tag="ACFB78_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC1269665.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            1012..1560
                     /locus_tag="ACFB78_001721"
     CDS             1012..1560
                     /locus_tag="ACFB78_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269666.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1567..4782
                     /gene="carB"
                     /locus_tag="ACFB78_001722"
     CDS             1567..4782
                     /gene="carB"
                     /locus_tag="ACFB78_001722"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC1269667.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4983..6023)
                     /gene="queA"
                     /locus_tag="ACFB78_001723"
     CDS             complement(4983..6023)
                     /gene="queA"
                     /locus_tag="ACFB78_001723"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC1269668.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6140..6397
                     /locus_tag="ACFB78_001724"
     CDS             6140..6397
                     /locus_tag="ACFB78_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC1269669.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQFRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6379..6840
                     /gene="accB"
                     /locus_tag="ACFB78_001725"
     CDS             6379..6840
                     /gene="accB"
                     /locus_tag="ACFB78_001725"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC1269670.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6952..8313
                     /gene="accC"
                     /locus_tag="ACFB78_001726"
     CDS             6952..8313
                     /gene="accC"
                     /locus_tag="ACFB78_001726"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC1269671.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8417..9304
                     /gene="prmA"
                     /locus_tag="ACFB78_001727"
     CDS             8417..9304
                     /gene="prmA"
                     /locus_tag="ACFB78_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC1269672.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRSGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLNGVKR"
     gene            9322..9885
                     /gene="orn"
                     /locus_tag="ACFB78_001728"
     CDS             9322..9885
                     /gene="orn"
                     /locus_tag="ACFB78_001728"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC1269673.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2544 a         2810 c         2518 g         2042 t
ORIGIN      
        1 gagagggcaa caagccgcaa ggcttgtatt tgggcggcga aggtattagg aaagattgcc
       61 accattcgga gaatgccctc cccccagccc tcccccacgg gggagggagt gggtttcggc
      121 agatttttcg gttgccggtg gtttgaaaaa taacttaaat ttgcaaccgt tgatttcagg
      181 tcgtctgaaa aaggatgcac gaaatatcaa cagcgggaat ttttcaggta gcctttattg
      241 tcaagcagat ggaacaaacg ccgcgagcgt tttttcagac aatctttgaa ctcatcggta
      301 gagtgtgtgc cgcaaggcgc gcatgcggtg ggtcggggtt acagggaaaa tgaagaacgc
      361 gtgcgtgcgt accgcacacc ctacatgcgg gctacggctt gctaggattg ctcgttatac
      421 ttaattttga caaggagtca atgaaatggc gatagaacta aatggtaact ggacaaaagg
      481 atttgcttta gaccatcata tgcaaaaaag tattttgtta ggttatgatg aattagggca
      541 tcctcacttt gatagcaaga gaagtgtgat tggagaatgg gtttatcaat taaaatatcg
      601 taatcaaacc caaaatgttt ctttgctggt agactatatt ttacaaaaat ttagtggttt
      661 agaatctata aatttaattg ttcccgcccc ttttacaaca gagcgtatta atcaaccagt
      721 tcaattaatt gccaaagaac taagtgatcg cttgaatatt ccatattctc ctattttaag
      781 aaaatctagc tcacacacac cactcaaaaa tattgaagaa aaatcagaaa aactagaaat
      841 actgaataat tccatcacaa ttgacaatat agatctatcc aataagaata ttttagtaat
      901 cgatgattta tttgatagcg gagcaacatt ggaaatatca actgaaaaat tgttttcaaa
      961 aaatgctaga tctgtaattg ttttagcaat gacaaagact agaggataaa aatgcgcact
     1021 attttctttt ctggttcacg cagcatttca cgactaaatc cacaaatacg agaaagaatt
     1081 aacaatattc ttagcaataa ttttgatatt gttattggag atgctaatgg ggctgataaa
     1141 gctatccaag aatttctaca agaacaagat tatgctaatg tgcatattta cttttctggc
     1201 aaaatttacc gaaataatgt gggaaattgg cagtttgtcc aagtagacag caaaggaact
     1261 ggaagggtat tttatacagc aaaagataag aaaatggcag aaattgctga ttatggcttt
     1321 attttatggg atggtaagag tattggttca ttaaataata tagcagaact tttacagcta
     1381 aataaacctt ctcttgtata ccattctcaa acaaaagaat tctttaaaat taaatcaagc
     1441 gcagatttag agaatatttt atctaacata gaagatgatg ttttagcttc aattttagaa
     1501 aagggaaata cttttcttaa aagctatgta acaaaacaac cttctttaat tcaagagtaa
     1561 ccacccatgc ccaaacgtac cgacctaaaa tccatcctta tcatcggcgc cggccctatc
     1621 gttatcggtc aggcctgcga atttgactat tcgggcgcac aggcctgcaa ggctttgcgt
     1681 gaagaaggct ataaagtcat tctggtgaat tccaaccccg ccacgatcat gaccgaccct
     1741 gaaatggcgg atgttaccta catcgaaccg attatgtggc agacggtgga gaagattatc
     1801 gccaaggagc ggcccgatgc gattctgccc acgatgggcg gtcagaccgc gctgaactgt
     1861 gcgctggatt tggcgcgtaa cggcgtgctg gcgaaataca atgtcgagtt aatcggcgca
     1921 acggaagacg cgatcgacaa ggcggaagac cgcggccgct ttaaagaagc gatggaaaaa
     1981 atcggcctct cttgcccgaa atcttttgtc tgccacacca tgaacgaagc cttggcggcg
     2041 caagaacagg tcggctttcc gacgctgatt cgtccgtctt tcacgatggg cggttcgggc
     2101 ggcggcattg cctacaataa ggatgagttt ttggcgattt gcgaacgcgg tttcgatgcg
     2161 tcgcctacgc atgagctgct gattgagcag tctgtgctcg gctggaaaga gtacgagatg
     2221 gaagtggtgc gcgataaggc ggacaactgc atcatcatct gttcgattga aaacttcgac
     2281 ccgatgggcg ttcatacggg cgactcgatt acggttgcgc cggcgcaaac gctgacggac
     2341 aaggaatacc aaatcatgcg caacgcttcg ttggcggtat tgcgcgaaat cggcgtggac
     2401 acgggcggct cgaacgtgca gtttgcggtg aaccctgaaa acggcgagat gattgtgatc
     2461 gagatgaacc cgcgcgtgag ccgttcgtcc gcgctggctt ccaaagcaac gggcttcccg
     2521 attgcgaagg tggcggcgaa gctggcggtc ggctttacgc tggacgagtt gcgcaacgac
     2581 atcaccggcg gccgcacgcc cgcgtcgttc gagccttcca tcgactatgt ggtaaccaaa
     2641 atcccgcgtt tcgcgtttga aaaattcccc gccgcagacg accgcctgac cacgcagatg
     2701 aaatctgtgg gcgaagtaat ggcgatgggc cgtaccattc aggaaagctt ccaaaaagcc
     2761 ttgcgcggtt tggaaaccgg cttgtgcggt ttcaatccgc gcagcgaaga caaagcggaa
     2821 atccgccgcg aactggccaa ccccggcccc gaacgtatgc tgtttgtggc agacgcgttc
     2881 cgcgcgggct tcacgccgga agaaatccac gagatctgcg ccatcgaccc ttggttcttg
     2941 gcgcaaatcg aagacttgat gaaggaagaa aaatcggttt cagacggcca gttgcaagat
     3001 ttggattacg ccgccctacg ccgtctgaaa cgcaaaggct tctccgacaa acgtttggca
     3061 caattgttga acgtaagcga aaaagaagtt cgcgaacacc gctacgcgct gaagctgcat
     3121 ccggtttaca aacgcgtcga tacctgcgcc gccgagttcg ccaccgaaac cgcctatctt
     3181 tactccactt acgaagaaga atgcgaatcc cgtccttccg accgtaaaaa agtgatgatt
     3241 ctcggcggcg gcccgaaccg catcggtcag ggcatcgagt ttgactactg ctgcgttcac
     3301 gccgcactcg ccctgcgcga atcgggcttt gaaacgatta tggtcaactg caaccccgaa
     3361 accgtgtcca ccgacttcga caccagcgac cgcctctatt tcgagccgct gacattggaa
     3421 gacgtgttgg aaatcgtccg caccgaaaac ccgtggggcg tgattgttca ttacggcggt
     3481 caaaccccgc tgaaactcgc caacgcattg gttgaaaacg gcgtgaacat catcggcaca
     3541 tccgccgaca gcatcgatgc cgccgaagac cgcgaacgct tccaaaaagt gttgaacgac
     3601 ttaggcctgc gccaaccgcc caaccgcatc gcccacaacg aagaagaagc actcgtcaaa
     3661 gccgaagaaa tcggttatcc gctggtcgtg cgcccgtctt atgttttggg cggacgcgcg
     3721 atgcagattg tccactccgc cgaagccttg caaaaataca tgcgcgaagc cgtgcaggtt
     3781 tccgaagaca gccccgtgct gctcgatttc ttcctcaaca acgcgattga agtcgatgtg
     3841 gactgcgttt cagacggcaa agatgtggtt atcggcggca tcatgcagca cgtcgaacag
     3901 gcgggcatcc actccggcga ctccggctgc tcgctgccgc cctactcctt aagcgaagaa
     3961 atccaagacg aaatccgccg ccaaaccaaa gcgatggcgt acgcgctggg cgtggtcgga
     4021 ctgatgaacg tgcagtttgc cgtgcaggac ggcgtggtgt tcgtgttgga agtcaacccg
     4081 cgcgccagcc gtaccgtccc ctttgtctcc aaagccaccg gcgtgccgct cgccaaagtc
     4141 ggcgcgcgct gcatggcagg catttccctg aaagaacaag gcgtggaaaa agaagtcgtc
     4201 cccgatttct atgccgttaa agaagccgtg ttcccattca tcaaattccc gggcgtggat
     4261 acgattttga gtccggaaat gcgctccacc ggcgaagtga tgggcgtggg cgcaagtttc
     4321 ggcgaagcct actacaaagc ccaactcggc gcgggcgaac gcctcaaccc gaccggcaaa
     4381 atcttcctct ccgtgcgcga agaagacaaa gaacgcgtca ttaaaaccgc caaaaacttc
     4441 caagccttag gctacggcat ctgcgccacg cgcggcacgg cgcaatacct gaccgaacac
     4501 gggctgattg tgcaggccat caacaaagtc cccgaaggcc gcccgcacat cggcgacgcg
     4561 ctgaaaaacg gcgaaatcgc actggtcgta aataccgttt ccagcgatcc gcaatccgtg
     4621 tccgacagcc acatcatccg ccaaagcgca ttgcagcaac gcgtgccgca atacaccacc
     4681 accgccggcg gcgaagcgat gagcgaaggc gcgaaaagcc gggattatct gggtgtgtac
     4741 agcgttcaag agctgcacgg tcgtttgaaa aaccgcaact gatgcctgaa tcaggttgaa
     4801 caatgccgtc tgaagccgtt ttgcggtttc agacggcatt ttgttgcaca tagccttcac
     4861 tttcttttga cctttaagca ccctaatttg gggcggtgga ttatccaact gccggacatc
     4921 cataaaaata ccgcccgaac cacggttcag acggcatctg ccttgcaacg gacagcagca
     4981 gtttaaagcc cgcccccttc gttccgcccc aaaaccatcg catctccgta gctgaagaaa
     5041 cggtattcac gttcaatcgc atgacggtac acggcgcgga tatgacccat acccgaaaac
     5101 gcgccgacca acatcagcag cgtcgatttc ggcagatgaa aattggtaac cagcctgtcg
     5161 acaacattaa aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg tccgtctttc
     5221 aaatatcccg ttgcgcgcgc ggcagactcg agggcgcgca tggaagtcgt gccgaccgcc
     5281 caggctttgt tcccccgggc ttttgccgcc tcaacggcag cgacggtttc agacggcact
     5341 tcaaaccatt cgctgtgcat tttgtgttct tcgattttat cgacgcgcac gggttggaat
     5401 gtccccgcac cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg
     5461 cgcaaaagtt cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat
     5521 ttggcataaa cagtttgata acggctgtcg tcgtccgcat cggcggcacg ttcgatataa
     5581 ggcggcaggg gcaggtgtcc gttctgttcc aaaagttcgt aaacggtttg accgccttca
     5641 aaacgcaggc agaacagttc gccctcacgc ccgaccatca cggcacggat accgccttca
     5701 aacaccagcc ccataccggg cttgggggac ttggacgaac ggatgtgcgc caatgcggta
     5761 tggttgtcca acacacgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca
     5821 aacagccgcg ccttcatgac tttggtgttg ttgaatacca aaacgtcgcc tgcctcgaca
     5881 taatcgggca aatcgccaaa cacccggtct tgcagcggca tatcgggcag cgcgaccaaa
     5941 agccggctgc tgccgcgcac ctcgggcgga tgctgggcaa tcaggtgttc gggcagggta
     6001 aagtcaaaat ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc
     6061 actttagccc tttttcagac ggcatctttg cccgaaaaac caacagatta gaataaacac
     6121 tcttgactcg gggcatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc
     6181 gccgtttttt gatattttac tggacattta ccgacaattt cgggaaaatg aacacaattt
     6241 cacggtcgtt tcccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc
     6301 gttgccgcaa gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca
     6361 gcctttaaag gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa
     6421 gaatcgggta tcgccgaaat cgaagtaacc gaaggcgaag aaaaagtccg cattacccgt
     6481 accattgcag ctgcggcagc tcccgtttac gccgcgcccg tacctgccgc cgcgccggcc
     6541 gtaacgccgg ccgccgcacc cgttgcggca tccgcgcccg ccgccgcgcc ggccgcccgc
     6601 gatttgtccg acgctcaaaa atcgcctatg gtcggcacgt tctaccgcgc gcccggcccg
     6661 aatgccgccg catttgtcga agtcggtcaa caggtcaaag ccggcgacac gctgtgcatc
     6721 atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac ggtcaaagaa
     6781 attctggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat catcggataa
     6841 tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc ccttcagcgt
     6901 tcccgcaccc ttttttacgg acgggttgcc ggaaccgcag gaaaggtcat catgctgaaa
     6961 aaagttttaa tcgccaaccg gggcgaaatc gccttgcgcg tactccgtgc ctgccgcgaa
     7021 atgggcattg ccaccgttgc cgtgcattcc gaagccgaca aaggcagcct gcacgtcaaa
     7081 ctcgccgacg aatccgtgtg catcggtcct gccgcttctg cgcaaagtta tctgaacatt
     7141 cccgccatca ttgccgccgc cgaagtaacc tgcgcggacg ctgtccaccc gggctacggc
     7201 ttccttgccg aaaacgccga tttcgccgaa caggtcgaac agtccggctt taccttcatc
     7261 ggcccgaaac ccgacaccat ccgcctgatg ggcgacaaag tctccgccaa acacgcgatg
     7321 atagcggcag gcgtgccctg cgtccccggc tctgacggcg cattgcccga cgatgatgcc
     7381 gaaatcctca aaatcgccga caaagtcggt tatcccgtga ttatcaaagc atcgggcggc
     7441 ggcggcgggc gcggtatgcg cgtggtcgag aaaaaagaag acctcctcca gtctgtcgaa
     7501 atgaccaagg ctgaagcggg cgcggcgttc ggcaacccga tggtttatat ggaacgctac
     7561 ctgcaacgcc cgcgccacgt cgaaatccaa gtgcttgccg acgaacacgg caacgccgtc
     7621 tatcttgccg agcgcgactg ttcgctgcaa cgccgccacc agaaagtcat cgaggaagca
     7681 cccgccccgt ttatcgatga agaggcacgc aaaaaaatcg gcaaagcctg taccgacgcg
     7741 tgcaaacgca tcggctaccg gggcgcgggc acgtttgaat ttttatacga agacggcgaa
     7801 tttttcttta tcgagatgaa cacgcgcgtt caggtcgagc atccggttac cgaactcatc
     7861 accggcgtgg acatcgtcca agagcaactc cgcatcgcat ccggcctgcc gctgcaatac
     7921 aaacagaaag acatcaaaat cgaaggccac gccttcgagt gccgcatcaa tgccgaagac
     7981 ccgtacaact tcatccccag cccgggcccg attgaaagct gccacctgcc cggcggcttc
     8041 ggcatccgcg tggacagcca catctaccaa ggctaccgca tcccgccgta ctacgacagc
     8101 ctgatcggca aaatctgcgt acacggaaaa acgcgtgaac aggcaatggc gaaaatgcgc
     8161 gtcgcactcg ccgagctggc ggtaaccggc atcaaaacca atacgccgtt gcaccgcgac
     8221 ctgttcgccg atgcgggttt ccaagaaggc ggagtgagca tccattacct ggagcactgg
     8281 ctggaagcgc gcaaaaccaa acaggacaaa taagccgccg ccgatatgcc gtctgaagcc
     8341 gcccttccgc gttcagacgg catttccctt gccccgccgt ctgaaaccga tttcgatatt
     8401 tccaagaaag cccgttatgc cctaccaaca aatcaccgtc aacgtcaacg atgccgtcgc
     8461 cgaacgcctc gccgacgcgc tgatggaaca cggtgcactc tccgccgcca tcgaagatgc
     8521 ctgcgccggc acgcaaaacg aacaggcgat tttcggcgaa cccggtatgc ccaccgaaca
     8581 aatctggcag cagagcaaag tcatcgccct gttcggcgaa cacgacgaag ccgccgccgt
     8641 catcgatgcc gccgcacaag aatgcgggtt aaaagacttg gcatacaccg gcgaaaccat
     8701 cgaagaccaa gactgggtgc gcctcacgca atcgcaattc gaccccatcc gaatttccga
     8761 ccgcctgtgg attaccccct cttggcacga agcccccgaa ggctgcgccg tcaacctccg
     8821 cctcgatccc ggactcgcct tcggcaccgg cagccacccg accacgcgcc tctgcctcaa
     8881 atggctggat acgcaactca aaaacggcga aagcgtcctc gactacggct gcggttcggg
     8941 catcctgacc atcgccgccc tcaaactcgg tgcaggttcc gccgtcggcg tggatattga
     9001 cgaacaggcc gtccgctccg gcagggacaa cgccgaacag aacaacgtcg atgcacaatt
     9061 cttcctgccc gacagtctgc ctcaagggca attcgacgta gttgtcgcca acatcctcgc
     9121 caaccctttg cgtatgctcg gcgaaatgct tgcagcccgc accaaacagg gcggacgcat
     9181 cgtgttgtcc ggtttgttgg atgaacaggc cgaagaactc ggcggcattt acagccaatg
     9241 gttcgatctc gacccggcgg aaactgacga aggctgggcg cgcttgaacg gcgtaaaacg
     9301 ctgaaacgga aaggaaacac cgtgcaggac aaaaacaacc tctgctggct cgatatggaa
     9361 atgacggggc tgaatcccga aaccgaccgc attatcgaag tcgcgatgat tattaccgac
     9421 tcggatttga atgtgttggc gcaatccgaa gtttacgcca tccaccaaag cgacgatctg
     9481 ctcgacaata tggacgagtg gaacaccgcc acacacggca ggacggggct gacgcagcgc
     9541 gtgcgcgaat cgtcgcatac cgaagccgaa gtcgaacaga aactgctgga ctttatgtcg
     9601 gaatggatac ccggacgcgc cacaccgatg tgcggcaact ccatccacca agaccggcgt
     9661 tttatggtca aatatatgcc gaaactggaa aactacttcc actaccgcaa cctcgacgtt
     9721 tccacgctga aagaactcgc caaacgctgg aatccgccca ttgccaaaag cgtcgtcaaa
     9781 cgcggttcgc acaaggcatt ggacgacatt ttggagagca tcgaagaaat gcgccactac
     9841 cgcgaacact tcctgatttc cgccccgaaa gccgaagcgc aataagaaac aaacaatgcc
     9901 gtctgaaacg ctat
//
LOCUS       DAXDZG010000075         9838 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXDZG010000075 DAXDZG010000000
VERSION     DAXDZG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.075"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            104..1369
                     /gene="efeB"
                     /locus_tag="ACFB78_001729"
     CDS             104..1369
                     /gene="efeB"
                     /locus_tag="ACFB78_001729"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC1269674.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWGRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHMRLANPR
                     DPEFLKKHCLFRRAYSYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1441..1770)
                     /locus_tag="ACFB78_001730"
     CDS             complement(1441..1770)
                     /locus_tag="ACFB78_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC1269675.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1845..3215)
                     /gene="glmU"
                     /locus_tag="ACFB78_001731"
     CDS             complement(1845..3215)
                     /gene="glmU"
                     /locus_tag="ACFB78_001731"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC1269676.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAKLERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(3270..3545)
                     /locus_tag="ACFB78_001732"
     CDS             complement(3270..3545)
                     /locus_tag="ACFB78_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC1269677.1"
                     /translation="MRKTFLILMTPAALFLSGCAWETYQDGNGKTAVRQKYPAGTPVY
                     YQDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(3538..4251)
                     /locus_tag="ACFB78_001733"
     CDS             complement(3538..4251)
                     /locus_tag="ACFB78_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC1269678.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHAPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTNPENTKECYA"
     gene            4310..5311
                     /locus_tag="ACFB78_001734"
     CDS             4310..5311
                     /locus_tag="ACFB78_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1269679.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRDNPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5405..6253
                     /locus_tag="ACFB78_001735"
     CDS             5405..6253
                     /locus_tag="ACFB78_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC1269680.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            6280..6762
                     /locus_tag="ACFB78_001736"
     CDS             6280..6762
                     /locus_tag="ACFB78_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC1269681.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(6846..8414)
                     /gene="msrAB"
                     /locus_tag="ACFB78_001737"
     CDS             complement(6846..8414)
                     /gene="msrAB"
                     /locus_tag="ACFB78_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC1269682.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGTATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            8560..9813
                     /gene="ftsY"
                     /locus_tag="ACFB78_001738"
     CDS             8560..9813
                     /gene="ftsY"
                     /locus_tag="ACFB78_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC1269683.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEVAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2032 a         2587 c         2984 g         2235 t
ORIGIN      
        1 cgccatatgt tgcagggcgg gctttcagac cgccgctatc ggttaatgga aaaacggcgt
       61 gcagggatgc ccaccctgct gcacggatat tgaaggaaac gccatgagtc aaaaccaacc
      121 cgcacaaccg accaaacgca atctgttcaa aaccgccctt gccgtcggcg caatcggcgc
      181 aatcggaggt tatttcggcg gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag
      241 ccaacactcg ccccaagcct atccctgcta cggcgaacat caggcaggta tcgttacgcc
      301 gcggcaggcg ttttccatta tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct
      361 ggaaaacctg ttccgcacac tgaccgcccg catcgagttt ctcacccaag gcggagaata
      421 ccaagacggc gacgacaaac tcccgtcagc cggcagcggc attttgggca aagccttcaa
      481 ccccgacgga ttgaccgtta ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg
      541 actcaaagac aaaaaaacgg ttcatttgca ggaaatgcgc gacttcccca acgataagct
      601 gcaaaaaagc tggtgcgacg gcgatttgag cctgcaaatc tgcgccttca cccccgaaac
      661 ctgccaagcc gccctgcgcg acatcatcaa acacaccgcc caaaccgccg tcatccgctg
      721 gagtatcgac gggtggcagc ctaaatccga acccggcgcg atggcggcgc gcaacctgtt
      781 gggcttccga gacggcacgg gcaaccccaa ggtttccgat cccaaaaccg ccgacgaggt
      841 tttatggacg ggcgtggccg ccaacagcct cgacgaaccg gagtgggcga aaaacggcag
      901 ctatcaggca gtccgcctta tccgccgctt tgtcgagttt tggggcagga cgccgcttca
      961 agagcaaacc gacattttcg ggcggcgaaa atacagcggg gcgccgatgg acggcaaaaa
     1021 agaagccgac caaccggatt tcgccaaaga ccccgagggt gatatcacgc ccaaagacag
     1081 ccatatgcgc ctggcgaatc cgcgcgatcc cgaattcctc aaaaaacact gcctcttccg
     1141 ccgcgcctac agctattctc gcggacccgc ctcaagcgga cagcttgatg tcgggctggt
     1201 gttcgtctgc tatcaggcaa atcttgccga cggtttcatc ttcgtgcaaa acctcctcaa
     1261 cggcgaaccg ctggaagaat acatcagccc cttcggcggc ggctatttct tcgtcttgcc
     1321 cggcgtggga aaaggcggat tcttgggaca agggctgccg ggcgtataaa cctgcctgac
     1381 ggacaaatgc cgtccgaacc ttgccaaacg ggttcggacg gcattttact gtttggacgg
     1441 tcaggctttt ttgacgaatt cggattttaa attcatcgcg ctgccgtcga ttttgcagcc
     1501 gatgttgtga tcgccttcct gcaggcgtat gcctttgacc ttcgtacctt gtttgatcgt
     1561 catcgagctg ccttttacct tgaggtcttt gatgaggatg acggtgtcgc cgttttgcag
     1621 cgccgcgccg ttggcatcgc gcacttgagc cgcaaggtcg gcggcggatt cggtttcatt
     1681 ccattcgtgg gcgcattcgg ggcagatgta ttgtccgccg tcttcatagg tgtattcgga
     1741 ggcgcattgc gggcaagggg gtaatgacat ggtttgccgt ccttatcgga tgtttgtttt
     1801 gggatgccgt ctgaaacctg aaaccggctt cagacggcac ggctttattg tttgtctttt
     1861 tccggcctca tccagccttc gatgacggtt tggcgggcgc gggcgagggc gagtttgttg
     1921 tcttcgatat tgcgggtaat cgtgctgcct gcgcctgtgg ttactttgtt gcccagtgtt
     1981 acaggggcaa ccagtacgca gttcgaaccg atgcgcactt cgtcgccgat gacggttttg
     2041 tgtttgtgca cgccgtcgta gttggcaata atcgtgcccg cgccgaagtt ggttttgctg
     2101 ccgacttcgg cgtcgcctat gtaggtgagg tggttggctt tggtgccttt gccgatggcg
     2161 gcgtttttga tttcgacgaa gttgccgacg tgtacattgt cagccagttt ggcttgcgga
     2221 cgcaggcggg cgtacgggcc gattcggttg ttttcgccga cttcgcagcc ttcgaggtgg
     2281 gagaaggggg caattttgct gtttgcgccg attttggcgt ttttgatgac gcagtttgcg
     2341 ccgatttcga cgttgtcgcc gagttcgact tcaccttcga tgacgacgtt gacgtcaatc
     2401 acgacatcct gtccgtgttt cagacggccc cgcaaatcga aacgcgccgg atcgcgcagg
     2461 gtcacgcctg ctttgagcaa ttcttgcgcc tgttcggttt ggaagatgcg ttcgagtttg
     2521 gcgagctggc gtttgttgtt cacgccggcg gcgaggtggg aggcgcgcac ccggacgggg
     2581 cggactttga tgccgtcggc aacggctttg gcgatgaggt cggttaaata atattcgcct
     2641 tgggcattgt tgctggaaag gctgttcagc cagttttcca gtttggcgtt gggcaggacg
     2701 aggatgcctg tgttgatttc gcggatggtt ttttgggtgg cacttgcgtc tttttcttcg
     2761 acaatggcgg ttacgctgcc gctgccgtcg cggatgatgc gccccaagcc tgtcgggtcg
     2821 gcgggcacgt cggtcaacag cccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg
     2881 gtttcaacgt caattaaagg aacgtcgccg tacaacacca gcgtgcggcc ttcggaggca
     2941 aggtggggca gggcggtttt gacggcgtgg ccggtgccga gttgttcggt ttgttcgacc
     3001 cagacggcat cgcgtttgac agtgtctaaa acttgctctt tgccgtgtcc gacgacgacg
     3061 cagatgtttt gcggattcag cgcggcggcg gtgtcgataa cgcgctcgac catgggcttg
     3121 ccgccgatgc agtgcagcac tttgggcatt tgggaataca tgcgcgtgcc tttgccggcg
     3181 gcgaggatga cggtgtttaa agtgttttgc ggcatagcgt tttcctgtgc catgccgtct
     3241 gaagcggttt cagacggcat agggtaggtt tatcggtttt gaaactttgg tttttgccag
     3301 tgttggcgat gctcttcgtc ggcgttgttg ccggtttggt cgggcagcac ggcgcggcgt
     3361 tcgggacggt attggttgta gttcatattt ttcgagtagc tgccgtcttg gtaataaacg
     3421 ggcgtgccgg cgggatattt ttgacggacg gcggttttgc cgttgccgtc ttgataagtt
     3481 tcccacgcgc agcccgacaa aaaaagggcg gcgggggtca tcaggataag gaaggtttta
     3541 cgcataacat tctttcgtat tttcggggtt tgtgcgggta ttgtaatgat tttggcggcg
     3601 ttctgacaaa gtttctgcat accgtgccag ttgcgccata tcgcttacgg aggcatcgat
     3661 aaagggcggt gcgtgggatt ttgcaccgaa ccggacggtt ttcatgccca atgcttttgc
     3721 ctgatggagg ttgtccgcgc tgtcgtccac cataatgcag cattcgggcg gtacgtccaa
     3781 caggcggcag acattgagat acgcttgcgg attgggtttg tacagcagtc cgaaatcatc
     3841 cgtgccgaac agtgcgtcga aacagttttc caaaccgagt gcgccggcaa cggcacggac
     3901 gtaaaacgac gggccgttgg aaaaaaccgc cttgcgcccg ttcagacggc atagggtgtt
     3961 ttccgtatcc gccatgccgt gcagcctggt caggattgca tcgatcggat ggctttcgcg
     4021 taaaaattcc gcaatgtcga tttcgggatg gtggatttgc agtccggcga gcgttgcgcc
     4081 gtaacggtgc caatagtctt gacgcaggtc ggacgcggcg gattcggaga gtttgaggcg
     4141 gcgtgccata tagcgcgtca tagcgcggtt gatgaggtgg aagatgcctg cgtcggcatc
     4201 gtgcagcgtg ttgtcgaggt cgaacagcca cacggtcggg ttttcttgca ttttgaaccg
     4261 tgaaaatttg ttagaatgtt attttacagc gaataaagga agactcggaa tgaaacggaa
     4321 aatttggctg ctgccgctgc tggcggtttc ggcatacctg caggcgcaga cggaagtcag
     4381 gctggcggtg cataagtcgt tcagcctgcc caaagggctg attgcgcgct tcgagcgggc
     4441 aaacgatgcg aaagtgtcga ttattcaggc gggcggcgcg aacgaaatgc tcaacaaact
     4501 gattttgagc cgagacaacc cgattgccga cgcggtgtac ggtttggaca acgccaatat
     4561 cggcaaggcg cgggaaatgg gcattttggc ggcggcgcaa cccgaatccg cccccgtcgc
     4621 ggtcgggctg ccttcggctt tggcggtcga ttacggctat gtgtccgtca attacgacaa
     4681 aaaatggttt gaaggcaaaa agctgcccct gccgcaaacc ctgcaggatt tgacccgccc
     4741 cgaatataaa aacctattgg tcgtgccgtc ccccgccaca tcttcccccg gcttgggctt
     4801 cctgatggcg aacatcggcg gtctgggcga agaaggcgcg tttaaatggt gggcacagat
     4861 gcggcagaac ggcgtgaagg tcgccaaagg ctggagcgag gcgtattaca ccgacttttc
     4921 gcacaacggc ggcgcgtatc cgctggtggt cggttatgcc gccagccctg cggcggaagt
     4981 atatttttcc aaaggcaaat acagcgagcc gccgacgggc aacctgtttt taaaaggcgg
     5041 cgtattccgt caggtcgaag gcgcggcggt cttgaagggc gcgaaacagc cgaagctggc
     5101 ggcaaaactg gtgcaatggc tgcaaagtcc ggaagtgcag caggcggttc cgtctgaaat
     5161 gtgggtttac cccgccgtca aaaacacgcg cctgcccgac gtgttccgct tcgcccaagc
     5221 cccggcgcac actaccgccc ccgcgcagcg cgatattgat gcgaaccagc gcggatgggt
     5281 ttcccgttgg atcaggacgg ttttgaaata aaacaaacat accgccccgc agggcttcag
     5341 acggcatttt tacacctgtg ccgattacgc cgcacggggc ggatgttcga tcgagaggaa
     5401 aacaatggac ttcaaacaat ttgatttttt acacctgatc agtgtttccg gttgggggca
     5461 tctggctgaa aaggcgtggg cgttcgggct gaaccttgcc gccgcgctgc ttattttctt
     5521 ggtcgggaaa tgggcggcga aacgcattgt cgccgtgatg agggcggcga tgacgcgcgc
     5581 gcaggtcgat gccacgctga ttagtttttt gtgtaatgtt gccaatatcg gcttattgat
     5641 tttggtgatt attgccgcat tgggcagatt gggcgtttcc acaacatccg taaccgcctt
     5701 aatcggcggc gcgggtttgg cggtggcgtt gtccttaaaa gaccagctgt ccaattttgc
     5761 cgccggcgcg ctgattatcc tgttccgccc gttcaaagtc ggcgacttta tccgtgtcgg
     5821 cggttttgaa ggatatgtcc gggaaatcaa aatggtgcag acttctttgc ggacgaccga
     5881 caacgaagaa gtcgtgctgc ccaacagcgt ggtgatgggc aacagcatcg tcaaccgttc
     5941 cagcctgccg ctttgccgcg cccaagtgat agtcggcgtc gattacaact gcgatttgaa
     6001 agtggcgaaa gaggcggtgt tgaaagccgc cgccgaacac cccttgagcg ttcaaaacga
     6061 agagcggcag cccgccgcct acatcaccgc cttgggcgac aatgccatcg aaatcacatt
     6121 atgggcttgg gcaaacgaag cagaccgctg gacgctgcaa tgcgacttga acgaacaagt
     6181 ggtcgaaaac ctccgcaaag tcaatatcaa catcccgttc ccgcaacgcg acatacacat
     6241 catcaattct taaacgccgt ctgaaagggg gggtgggaaa tggacgcgtt gcacaccatc
     6301 gcccgaaacc tgacgaaaaa acgtcaaacc gtaagctgtg ccgaatcctg cacgggcgga
     6361 atgcttgccg ccgcattcac aagcgttgca ggcagttcgc aatggttcga ccagagtttt
     6421 gtaacataca gcaacaaagc caaagaatac cgcttgggcg tgttgcccga aaccctgctc
     6481 gaacacggcg cggtcagccg ccaaaccgtc tatgagatgg cgcgcggcgc gaaagccgtg
     6541 gctcaggcgg attacgccgt cggtatttcc ggcatcgccg gcccgggcgg cggcgaaagc
     6601 aaacccgtcg gcacggtttg gttcgggttt gcctttccgg gcggaagttg cgaagcaatg
     6661 cgccgttttg acggcaaccg cgaatccgtc cgcgcgcagg cggtcgcctt cgcgttggaa
     6721 cggttggccg ggctggttga aaacggcggc gatgctgtct gaacaaatcc ccgccggata
     6781 aaaaatgccg tctgaaacgt tgcaggtttc agacggcatt ttgccgggtt aggcggcggt
     6841 gcggcttatt tcactttgcc cttcaacgcg ccgtagcctg ccgcgtccat ttgttccagc
     6901 gggatgaatt tcaagctcgc gccgttgatg cagtagcgca gtccgccttt gtcgcgaggg
     6961 ccgtcgggga agacgtgtcc caagtgcgaa tcggcggcgc ggcttctgac ttcggtgcgg
     7021 cgcatattga agctgaaatc atcgtgttcg gtaacggatt ttgcatcaat cgggcgcgtg
     7081 aagctcggcc agccgcagcc ggaatcatat ttgtcggcgg agctgaacag gggttcgccg
     7141 ctgacaacgt ccacataaat gccgggtttg aacaaatggt cgtattcgtg gctgaaggcg
     7201 tattcggtcg cgctgttttg ggtcacttgg tattgctctt cggtcagggt gcgtttgagt
     7261 tcggcgtcac tcggtttttt atacgttgcc gcgtcgaagc ctttgccttg cggtgcggct
     7321 ttggttttgc ccggcagcgg ttcgtcggct ttgcggatgt cgatgtggca gtagccgttg
     7381 gggtttttaa tcaggtagtc ctgatggtat tcctcggcgt cgtagaagtt tttcagcggt
     7441 tcgttttcaa caacgagggg cagttggtat ttttgctgct cgcgtttgag ggcggcggcg
     7501 atgacggctt tttcggcggg gtcggtgtag tacacgccgc tgcggtattg cgtgccggtg
     7561 tcgttaccct gtttgttgag gctggtcgga tcaacgacgc ggaaataata ttgcaggatg
     7621 tcgtccaggc tgagtttgtc ggcatcgtag gtcactttga cggtctcggc atggcccgta
     7681 tggcggtagg acacgtcttc gtagctcggg ttttccgtgt tgccgttggc gtagccggat
     7741 accgcgtcaa ccacgccgtc gatgcgttgg aaataggctt ccaagcccca gaagcagccg
     7801 ccggcgaggt agatggtgcg cgtgttcatg attgctgaat cctttttctg agtgtcgggt
     7861 ttgtagaacg aatgtttcaa actgcccaaa tcggcattcg ggttgcggat taacgccaat
     7921 gcctgcgctt cgttgatgct gcctttgacg atgcgctgca cgtcgccgtc tttaccgatt
     7981 aacgcccaag aaggataaac gctgatattc aggttttggg cgatcgtgcc gccgttgtcg
     8041 gtaacgacgg gcagcttggg gtagttcaaa ccggcatacc atttttgaaa ctcgccgtct
     8101 tttttctcgt gcaaaaagcc gggggaggcg acggtaatca ggttggcgga gctgaatttt
     8161 gcatcttgcg cccatttctc ggcctgtccc aattcggaca aacataaagg acaccagctc
     8221 gcccaaaatt taatcagcgt cggtttgtct tttttcaaat aaacactggc ggggcggttg
     8281 tccgcggttt ttaacgtgga taaagtgtgc ggcacggtcg cggtcccggc atcgacgatt
     8341 ttgggcgaac aagcgcccag cgcaagcagg cagccgaact tggcgcaaag ggaaaagaaa
     8401 gtacggtgtt tcattttgat gtttcctgtg tggacggttt gcatgattag acgtttgaga
     8461 tgccgaaacc ttacggcccg gattttcaga caaccttgcc gcgcaaaata cgctacaata
     8521 cgccctattt caagtttcta aaattaaaag gaaaattcaa tgttcagctt cttccgtcgc
     8581 aagaaaaaac aggaaacgcc ggctctcgag gaggcccaag ttcaggaaac cgcagcaaaa
     8641 gtagaatctg aagttgctca aatagttgga aatattaaag aagatgtcga atctttagca
     8701 gaaagcgtca aagggcgggc cgaatctgcc gttgaaaccg tcagcggtgc ggttgaacag
     8761 gtaaaggaaa ccgttgccga gatgccgtct gaagcagggg aagtggcgga acgcgttgaa
     8821 tccgcaaaag aagctgttgc cgaaaccgtc ggcgaggctg tcgggcaagt tcaagaagcc
     8881 gttgcgacaa ctgaagaaca caagctcggt tgggctgcgc gtttgaaaca aggcttggcc
     8941 aaatcacgcg acaaaatggc gaaatcgctg gccggcgtgt tcggcggcgg acaaatcggc
     9001 gaggatttgt acgaagagct ggaaaccgtg ctgattaccg gcgatatggg catggaagcc
     9061 accgaatacc tgatgaaaga cgtgcgcggc cgcgtcagcc tcaaagggct gaaagacggc
     9121 aacgaattgc gcggcgcgtt gaaagaagcc ttgtacgacc tgattaagcc gttggaaaaa
     9181 ccgctggtct tgcccgaaac taaagagcct ttcgtgatta tgcttgccgg tatcaacggc
     9241 gcgggcaaaa ccacgtctat cggcaaactc gccaaatatt tccaagcgca gggcaaatcc
     9301 gtattgctgg cggcgggcga taccttccgc gccgccgccc gtgagcagct tcaggcttgg
     9361 ggcgggcgca acaatgtaac cgtcatttca caaaccacgg gcgattccgc cgccgtgtgc
     9421 ttcgatgccg tccaagccgc caaagcgcgc gggatcgaca tcgtgcttgc cgacaccgcc
     9481 ggccgcctgc ccacgcagct tcatttgatg gaagaaatca aaaaagtgaa gcgcgtgctg
     9541 caaaaagcca ttcccggcgc gccgcacgaa attatcgtcg tactcgatgc caatatcggg
     9601 caaaacgccg tcaaccaagt caaagccttt gacgacgcat tggggctgac ggggcttatc
     9661 gttaccaaac tcgacggcac ggcaaaaggc ggcatcctcg ccgcgcttgc ttccgaccgc
     9721 cccgtccccg tccgctacat cggcgtgggc gaaggcatag acgacctgcg cccgtttgac
     9781 gcgcgcgcgt ttgtggacgc actgctggat tgagccgaaa tgccgtccga aaacggca
//
LOCUS       DAXDZG010000076         9673 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXDZG010000076 DAXDZG010000000
VERSION     DAXDZG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.076"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB78_001739"
     CDS             complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB78_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC1269684.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(1820..2110)
                     /locus_tag="ACFB78_001740"
     CDS             complement(1820..2110)
                     /locus_tag="ACFB78_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC1269685.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2615..4780
                     /gene="fetA"
                     /locus_tag="ACFB78_001741"
                     /gene_synonym="frpB"
     CDS             2615..4780
                     /gene="fetA"
                     /locus_tag="ACFB78_001741"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC1269686.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKISDKEKGPNGTEVD
                     VDVAQKAKNRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSSLNPSFGVIWQPREHWNFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNTGYIKNH
                     GYELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            4932..5897
                     /locus_tag="ACFB78_001742"
     CDS             4932..5897
                     /locus_tag="ACFB78_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1269687.1"
                     /translation="MLRLTALTLCTALALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            5915..6106
                     /locus_tag="ACFB78_001743"
     CDS             5915..6106
                     /locus_tag="ACFB78_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269688.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            6125..7093
                     /locus_tag="ACFB78_001744"
     CDS             6125..7093
                     /locus_tag="ACFB78_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1269689.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGIADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFVAY
                     EFEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            7083..8057
                     /locus_tag="ACFB78_001745"
     CDS             7083..8057
                     /locus_tag="ACFB78_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC1269690.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            8102..8728
                     /locus_tag="ACFB78_001746"
     CDS             8102..8728
                     /locus_tag="ACFB78_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC1269691.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDSGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            8812..9570
                     /locus_tag="ACFB78_001747"
     CDS             8812..9570
                     /locus_tag="ACFB78_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1269692.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(9605..>9673)
                     /locus_tag="ACFB78_001748"
     CDS             complement(9605..>9673)
                     /locus_tag="ACFB78_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC1269693.1"
                     /translation="EFRFNSGTPSRQLKILRDRCGI"
BASE COUNT         2139 a         2839 c         2511 g         2184 t
ORIGIN      
        1 tttaaggttt gcgccttagc cgcacttatt cgcaaaaaaa ccgcacggcg ttgaccgtgc
       61 ggcttgttgt ctgaaggttt cagacggcat tgcttacatc atgccgccca taccgcccat
      121 tccccccata tcgggcacag ccggtttttc ttcagggatt tcggcaatca tgcagtcggt
      181 cgtcagcatc agaccggcga tagacgcggc gtgttgcagc gcggaacggg ttactttggc
      241 agggtcgagt acgcccattc cgatcatgtc gccgtattcg ccggagcctg cgttgtaacc
      301 gtagttgcct ttgccttcca acactttgtt caccaccacg ctgggttctc cgcctgcgtt
      361 ggcaacgatt tggcgcagcg gagactcaac ggcgcgcaat acgatttgta cgcctgcgtc
      421 ttggtcggca ttgccggtgt gcaggttttc caaagcggca cgggcgcgca acagggctac
      481 gccgccgcct gcaaccacgc cttcttcaac ggctgcgcgg gtagcgtgca gcgcgtcttc
      541 cacgcggtct tttttctctt tcatttcgac ttcggtcgcc gcgccgactt tgatcactgc
      601 cacgcctcct gccagtttgg caacgcgctc ttgcagtttt tctttgtcgt aatcgctggt
      661 cgcggtttcg atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc
      721 gaagccgtcg atgacggtag tgttttcttt accgatttcg atgcgtttgg cttgacccaa
      781 gtcgtccaaa gtcgcttttt ccaaagacag gccgacttct tcggaaatca ctacgccgcc
      841 ggtcaggatg gcgatgtctt gcagcatcgc tttgcggcgg tcgccgaagc cgggggcttt
      901 gacggcaacg gttttcagga tgccgcggat gttgttcacg accaaagtcg ccaaggcttc
      961 gccttctacg tcttcagcga taatcaacag cgggcggctg gctttcgcca cttgttccaa
     1021 cacgggcagc aggtcgcgga tgttgctgat ttttttgtcg aacagcaaaa caaacggatt
     1081 gtccagaccg gcgatttgtt tttccgcgtc gttgatgaag taaggggaca ggtagccgcg
     1141 gtcgaactgc ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac
     1201 ggtaatcacg ccttctttgc cgactttttc catcgcttcg gcgataatcg cgccgacttg
     1261 ttcgtcggag ttggcggaaa tcgagccgac ttgggcgatt tctttggaag tatcgcaagg
     1321 tttggcgatg tttttcagct cgtcaaccaa agcggcaacg gctttgtcga tgccgcgttt
     1381 cagatcggtc gggttcatgc cggcggtaac gtatttcatg ccttcggcaa cgatggattg
     1441 tgccaatacg gtggcggtag tcgtaccgtc gccggctacg tcgttggttt tggacgcgac
     1501 ttctttcacc atttgcgcgc ccatattttc aaacttgtct ttcagttcga tttctttggc
     1561 gacggttacg ccgtctttgg tgatgtgcgg gccgccgaaa gcgcggtcaa ccaccacgtt
     1621 gcggcctttg gggcccaagg ttacgcgcac ggcgtttgcc aaaatattca cgccgtttac
     1681 cattttttgg cggacttcat tgccgaattg tacgtctttt gctgccattt caattctcca
     1741 aaaatcttaa aactgtctga taaaaccgtt tatgccgtct gaaggcggtt tgccgtttca
     1801 gacggcatcg tgtccgtatt tatttttcaa cgatgccgaa aatatcttct tcgcgcatta
     1861 ccaacagctc ttcgccgtcg gcttttacgg tttggccgct gtatttgccg aaaatgattt
     1921 tgtcgccggc tttgacatcc agcggacggc gcgcgccgtc tttgccgatt ttgcccgcac
     1981 ccacggcgat gacttcgccc atatcgggtt tttcggcggc cgcgcccggc aggacgatgc
     2041 cagaggcggt tttttcttca gcttccaagc gtttgacgac gacgcggtcg tgtaaaggac
     2101 ggatggtcat atttatgctc cgataaatgg tttgaaaaca atcatctgcc cgaacggttc
     2161 ggacaaattg aagtggaaac cgggctgccg cctaacggcc tgcccgtaca agtcggcaaa
     2221 attagggtgt gtgcgggaaa attcaagtga ggcggaaaaa aatttatttc cgccgttttt
     2281 tatagtggac taaatttaaa ccggtacggc gttgcctcgc cttagctcaa agagaacgat
     2341 tccctaaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact gcctgcggct
     2401 tcgccgcctt gtcctgattt ttgttaatcc actatacatt tccgacaaaa cctgtcaaca
     2461 aaaaacaacg cttcgcaaat aaaaacgata atcagcttta cacaaccccc cccccgctaa
     2521 tataaacaaa aataattatt attatttttt cttatcctgc caaaccttaa cggtttggct
     2581 taacttccct tcatacactc aaaaggacga acaaatgaac gccccgtttt tccgcctcag
     2641 cctgctctcg ctcacacttg ccgccggctt tgcccacgcg gcagaaaata atgccaatgt
     2701 cgcattggat accgttaccg taaaaggcga ccgccaaggc agcaaaatcc gtaccaacat
     2761 cgttacgctt caacaaaaag acgaaagcac cgcaaccgat atgcgcgaac tcttaaaaga
     2821 agagccctcc atcgatttcg gcggcggcaa cggcacgtcc caattcctga cgctgcgcgg
     2881 tatgggtcag aactctgtcg acatcaaggt ggacaacgcc tattccgaca gccaaatcct
     2941 ttaccaccaa ggcagattta ttgtcgatcc cgctttggtt aaagtcgttt ccgtacagaa
     3001 aggcgcgggt tccgcctctg ccggtatcgg cgcgaccaac ggcgcgatca tcgccaaaac
     3061 cgtcgatgcc caagacctgc tcaaaggctt ggataaaaac tggggcgtgc gcctcaacag
     3121 cggctttgcc ggcaacaacg gcgtaagcta cggcgcaagc gtattcggaa aagagggcaa
     3181 cttcgacggt ttgttctctt acaaccgcaa cgatgaaaaa gattacgaag ccggcaaagg
     3241 tttccgcaat gacaacggcg gcaaaaccgt accgtacagc gcgctggaca aacgcagcta
     3301 cctcgccaaa atcggaacaa ccttcggcga cggcgaccac cgcatcgtat tgagccacat
     3361 gaaagaccaa caccggggca tccgcactgt gcgtgaagag tttgccgtca gcgaaaaaaa
     3421 ttcacggata actattaaac gccaagcccc atcctaccgc gaaaccactc aatccaacac
     3481 caacttggcg tacaccggca aagatttggg ctttgtcgaa aaactggatg ccaacgccta
     3541 tgtgttggaa aagaaacgct attccgccga tgacaaagat aacggctacg caggcaatgt
     3601 aaaaggcccc aaccataccc gaattaccac tcggggtatg aacttcaact tcgacagccg
     3661 ccttgccgaa caaaccctgt tgaaatacgg catcaactac cgccatcagg aaatcaaacc
     3721 gcaagcgttt ttgaacggcg aatttaagat ctccgataag gaaaaaggac ccaacggaac
     3781 agaagtcgac gtagatgtag cacaaaaagc aaaaaaccgt gcaaatgaaa aaattgttta
     3841 cgcctacaaa ctttccaacc cgaccaaaac cgataccggc gcgtatatcg aagccattca
     3901 cgagattgac ggctttaccc tgaccggcgg gctgcgttac gaccgcttca aggtaaaaac
     3961 ccacgacggc aaaaccgttt caagcagcag cctcaacccg agtttcggcg tgatttggca
     4021 gccgcgcgaa cactggaact tcagcgcgag ccacaactac gccagccgca gcccgcgcct
     4081 gtatgacgcg ctgcaaaccc acggcaagcg cggcatcatc tcgattgccg acggcaccaa
     4141 agccgaacgc gcgcgcaata ccgaaatcgg cttcaactac aacgacggca cgtttgccgc
     4201 aaacggcagc tacttccggc agaccatcaa agacgcgctt gccaatccgc aaaaccgcca
     4261 cgactccgtc gccgtccgcg aagccgtcaa caccggctac atcaaaaacc acggttacga
     4321 attgggcgcg tcctaccgca ccggcggcct gaccgccaaa gtcggcgtaa gccgcagcaa
     4381 accgcgcttt tacgataccc atcctaaaaa actgttgagc gcgaaccccg agtttggcgc
     4441 acaaaccggc cgcacttgga cggcctccct tgcctaccgc ttcaaaaacc cgaatctgga
     4501 aatcggctgg cgcggacgct atgttcaaaa agctacgggt tcgatattgg cggcagggca
     4561 aaaagaccgc gacggcaaat tggaaaacgt tgtacgccaa ggtttcggtg tgaacgacgt
     4621 cttcgccaac tggaaaccat tgggcaaaga cacgctcaat gtcaatcttt cggttaacaa
     4681 cgtgttcgac aagttctact atccgcacag ccaacgctgg accaataccc tgccgggcgt
     4741 gggacgtgat gtacgcctgg gcgtgaacta caagttctaa aacgcacatc ccgaaaaaat
     4801 gccgtctgaa agcctttcag acggcatctg tcctgataat ttgatataga ttatcattta
     4861 tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata tccccatttt
     4921 ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgccctc gccttgggcg
     4981 cgtgttcgcc gcaaaattcc gaccctgccc cacaagccaa agaacaggcg gtttccgccg
     5041 cacaatccga aagcgcgtcc gttaccgtca aaaccgcgcg cggcgacgtt caaataccgc
     5101 aaaaccccga acgcatcgcc gtttacgatt tgggtatgct cgacaccttg agcaaactgg
     5161 gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag gaatatttca
     5221 aaacgaccaa acctgccgga actttgttcg agccggatta cgaaacgctc aatgcctaca
     5281 agccgcagct catcatcatc ggcagccgcg cagccaaggc gtttgacaaa ttgaacgaaa
     5341 tcgcgccgac catcgagatg accgccgata ccgccaatct caaagaaagt gccaaagagc
     5401 gtatcgacgc gctggcgcaa atcttcggca aacaggcgga agccgacaag ctgaaggcgg
     5461 aaatcgacgc gtcttttgaa gcggcgaaaa ccgccgcgca aggcaaaggc aaaggtttgg
     5521 tggttttggt caacggcggc aagatgtccg ccttcggccc gtcttcacga ctgggcggct
     5581 ggctgcacaa agacatcggc gttcccgctg tcgatgaatc aattaaagaa ggcagccacg
     5641 gccagcctat cagcttcgaa tacttgaaag agaaaaatcc cgactggctg ttcgtcctcg
     5701 accgcagcgc ggccatcggc gaagagggtc aggcggcgaa agacgtgttg aacaatccgc
     5761 tggttgccga aacgaccgca tggaaaaaag gacaggtcgt ttacctcgtt cccgaaactt
     5821 atttggcagc cggcggcgcg caagagctgc tgaacgcgag caaacaggtt gcggatgctt
     5881 ttaacgccgc gaaataatga aacggcggca ttcgatgccg tctgaaacac ggatgcaaac
     5941 cgcttcctgt gtttcagacg gcattgcccg atacgggcgt tatcgcggga atggcggcaa
     6001 gcgttttgat tggaacttgc cccatcctct gcccgtccgg aggtttcaaa caaggctttc
     6061 cgctccgacg gttcggactg ccttgtttga atctcctacg ccgtaacgct tttcccttct
     6121 gtttatgact gccaaacctt tttccctcaa cctggccaac ctcctgctgc cggcggtgtt
     6181 gtttgccgtc agcctgtcgg tcggcattgc cgatttccgc tggtcggatg tgttttcgct
     6241 gtccgacagc caacaggtca tgttcatcag ccgcctgccg cgcacgtttg cgattgtgtt
     6301 gacgggcgcg tcgatggcgg tggcggggat gattatgcag attctgatgc gcaaccgttt
     6361 tgtcgagcct tctatggcgg gtgcgggcca aagtgcggct ttgggtttgc ttctgatgtc
     6421 cctgctgctg cctgccgcgc cgctgccggt caaaatgtcg gttgccgccg ttgccgcgct
     6481 gatcgggatg ttggtcttta tgctgctgat ccgccgcctg ccgccgacgg cgcagctgat
     6541 ggtgccgctg gtggggatta ttttcggcgg cgtggttgag gcggtggcga cgtttgtcgc
     6601 gtatgagttt gagatgctgc aaatgttggg cgtgtggcag cagggcgact tttcaagcgt
     6661 gctgctgggg cggtacgagc tgctttggat tacgggcggt ttggcggtgt ttgcctacct
     6721 gattgccgac cggctgacga ttttggggct gggcgagacg gtcagcgtga atttgggttt
     6781 gaaccggacg gcggtgttgt ggtcgggttt gattattgtg gcactgatta catcgctggt
     6841 cattgtaacg gtcggcaata ttccgtttat cgggctggtc gtgccgaata tcgtcagccg
     6901 cctgatgggc gacaggctgc gccaaagcct gcctgcggtc gccctcttgg gcgcgtcttt
     6961 ggttttattg tgcgacatta tcggacgcgt gattgtgttt ccgtttgaaa ttccggtctc
     7021 tacggttttt ggtgtgttgg gtacggcttt gtttttgtgg cttttattaa ggaaacctgc
     7081 ccatgccgtc tgaaaaaaat atcggtttta tggcaggaag cagccgtccg ttgcgggtcg
     7141 cctttgcgct gttgctggtt tcctgcatcc tgtttatgac ggtcaacgtc aaaggcgatt
     7201 gggactttgt cttgcacctg cgcctgacca agcttgccgc gctgctgatg gtcgcctatg
     7261 cggtcggcgt gtccactcaa ctcttccaaa cgctgaccaa caacccgatt ctgacccctt
     7321 cgattttggg tttcgattcg ctgtatgtgt ttttgcagac cttgctggtg tttacgttcg
     7381 gcggcgtggg ctatacatcc ctgccgttga cgggcaaatt cggctttgaa ctggttgtta
     7441 tgatgggcgg ctcgctgctg ctgttttaca cgctcatccg tcagggcggg cgcgatttgc
     7501 cgcacatgat tttaatcggc gtgattttcg ggattttgtt ccgcagcctt tcctcgctgc
     7561 tttcgcgcat gatagacccc gaagaattta ccgccgcgca ggcgaatatg tttgccggat
     7621 tcaataccgt ccgcagcgag cttttaggca taggcgcgct ggtcctgctc gtcagcgcgg
     7681 cggtcgtttg gcgcgaacgc taccgctcgg acgtacacct tttggggcgc gaccaagccg
     7741 tcaatttggg catcagctac acgcgcaaca ccttatggat actgctttgg attgccgcat
     7801 tggtggcgac ggcgaccgcc gttgtcggcc cggtgagctt tttcgggctt ctcgccgcct
     7861 cgcttgccaa ccacttttcc ccgtccgtgc gccattccgt ccgcctgccg atgacggttt
     7921 gcgtcggcgg catcctcttg gtcggcggac aaaccgtatt cgaacacttc ttgggcatga
     7981 aggcggtatt aagcgtggtg gtcgaatttg cgggcggact cgttttcctc tatctcgttt
     8041 taaaacacaa aaaatgacgg atgccgtctg aacggcagcc cgccccgaaa ggacaaacca
     8101 tatgacgcaa gaacgtttac ccgaattttt cgaccgcgcc ccgacgctga ccgtacaaga
     8161 cccgcttgcc gcattcctcg gcgcggccga aaacggcatc ctcacttacc gctacgccga
     8221 tgccgtgcgc ctgtgcggac attcctgccc gaccgtcgcg ggcgcgtacc tgatggttat
     8281 caaaggcctg aaagcacttt acggcgaaga gctgcccgaa cgcggcggca tcgaagccgc
     8341 catgcaggga acgcgcgacg aaggcacggt cggcgtaacc gcgtccgtcg tccaactcct
     8401 taccggcgca gcccccgaaa ccggcttcgg aggcatcgga atgcagggac gcttcgcccg
     8461 ccgcaacctc ttatcctttg gtgcaggcga aatcaacggc acactgaccc tgcgccgcaa
     8521 agacagcggc aaaaccgtcg ccgtcagcct caacgccgcc ctgcaaccct ttgcacctca
     8581 aatgcgcgac atcatgccca aagccgtcgg cggcagcgca agcgcaaaag aactcgaacg
     8641 cttcggacaa ctttggcagg cacgcgttaa agcatttttg accgaatcgg cggacgaccc
     8701 gcagttcgtc atcgtccgcg aactgtgaac gttcagacgg cattccgaat ttcaaatgcc
     8761 gtccgaaccc ccgccaaaca acaacaaacc tacgcccgac aagcatccgc catgattacc
     8821 atccgcaacg tcagctaccg catcggcaca caccccatcc tcgacaacgt cagcctcgac
     8881 atccccgaag gcggcattac cgcgctaatc ggccccaacg gtgcgggcaa atccaccctg
     8941 ttttccttta tggcgcggct gcgcccgctt gaaagcggca gcatcgccta ccgaggcaaa
     9001 aaccttgccg gcacccccac cgccgaactc gcccgcacgc tctccatcct cacccaagaa
     9061 aacagcatca tgagccgcat caccgtgcgc gacctgctga tgttcggccg ttacccctac
     9121 catcaaggca gaccgactgc cgaatgccgc cgtatcgtta acggtgcaat cgaagaattc
     9181 cacctgcagg acctctccga ccgctacctg accgaactct ccggcggaca acgccaacgc
     9241 gccatgattg cgatggtgtt ctgccaaagc accgactacg tccttttgga cgaaccgctg
     9301 aacaacctcg atatgtatca cgcccgctcg ctcatgcaga ttctgcaaaa gctcacgcac
     9361 gaacacaaac gcaccaccgt cgccgtattg cacgacatca accaggccgc cgcctacgcc
     9421 gaccacgtcg tcgccatgaa aaacggcaaa gccgccctga ccggcacacc cgaagaagtg
     9481 ttcaccgtac acaacatcaa agaactgttc gacatggacg tggacgtact cgactacgaa
     9541 ggtaaaaaac tcatcgtcca ccacatctga agctcaaaaa gaaaggggct gtactagatt
     9601 atccctaaat tccacaccga tcccgcagga tttttagctg ccgggacggt gtgccggagt
     9661 taaatcgaaa ttc
//
LOCUS       DAXDZG010000077         9292 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXDZG010000077 DAXDZG010000000
VERSION     DAXDZG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.077"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            293..1147
                     /locus_tag="ACFB78_001749"
     CDS             293..1147
                     /locus_tag="ACFB78_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFC1269694.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1237..1695
                     /locus_tag="ACFB78_001750"
     CDS             1237..1695
                     /locus_tag="ACFB78_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1269695.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFB78_001751"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFB78_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC1269696.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5211)
                     /locus_tag="ACFB78_001752"
     CDS             complement(3688..5211)
                     /locus_tag="ACFB78_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC1269697.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVNMLDKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLKQTGLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAVYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(5518..6324)
                     /locus_tag="ACFB78_001753"
     CDS             complement(5518..6324)
                     /locus_tag="ACFB78_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC1269698.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6376..6627)
                     /locus_tag="ACFB78_001754"
     CDS             complement(6376..6627)
                     /locus_tag="ACFB78_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269699.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6729..7499
                     /locus_tag="ACFB78_001755"
     CDS             6729..7499
                     /locus_tag="ACFB78_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269700.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7645..8814
                     /gene="metZ"
                     /locus_tag="ACFB78_001756"
     CDS             7645..8814
                     /gene="metZ"
                     /locus_tag="ACFB78_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC1269701.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8909..9115
                     /locus_tag="ACFB78_001757"
     CDS             8909..9115
                     /locus_tag="ACFB78_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC1269702.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2127 a         2281 c         2713 g         2171 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgt tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa cgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataacggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga cgatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agactcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gccttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccgac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggccagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca tagcgagttt
     4741 ggatttgttt tcagagccgc cgccttttgc cgcgcaggtt acttcgactt taccgcccgg
     4801 cacgatgctc atgtggatga cggcgggggt gttgtctttg gtgttttggc gtttgccggc
     4861 cggatcggcg aggacggaag cgcgcagggt gttgccttcc caagtgtagg cgcggcgtac
     4921 gccttcgtta accatctctt ccacgctcat gtccgcatcc cattgcacat ccataccgac
     4981 tttgaggaag acggttgcga tacctgtgtc ttggcagatg gggcggttgt tttcggcaca
     5041 catacggctg ttgaccaaaa tctgcgtcat cgcgtctttg gcggcgggat tttcttcctt
     5101 ctgccacgcc ttataaagcg cgtcgatgta gtcttttgga tggtagtagc tgatgaattg
     5161 gaaggcatcg cagatacttt gaataaagtc ttcttgcttg ataacggtca tgattgtgtt
     5221 ccttttcaag gttgtggcgg taagtgtctg ctgctgtttt aaacaacctt tttgaaggct
     5281 gttttttatc aggttgaaat agtgccagcg tttgtacggt gaagacaata cataattcta
     5341 atctcttgat tatcaatttg ttttaataaa atactgtttt attcggattt tgatatttgt
     5401 aatctttgcc tgttgctttg cggtagggag aaatcagcag gcagttcatt gtggaccggt
     5461 tgatgaacaa tgccgtctga aagggcttca gacggcattt tgttcggacg ggcgggctta
     5521 tttcgcagcc tgtccgccct ctttggcaaa atatttggcg tagatcttgt cgtattcgcc
     5581 gctttcgcgt actttttcca acgcatcgtt cagcattttg acggttgctt cgtcgccttt
     5641 gcgtaccgcg atgccgtagt gttcggtggt gaagtcgggc agggtaacga agtccattcc
     5701 tttggccggg ttgtttttca cataattggc gatgaccgcg ctgtcgctga ccacggaatc
     5761 caagccgccg ttttccagtt ctttgataat cagggggacg ttttcaaagc gcgcgatttt
     5821 cggattgtcg ttgcccaaga gtttggaaac ggagaaatcg cccgtgtggc cggtaaccac
     5881 gccgactttg ttcatctttt tcaaatcttc ggaagaagat acttttttgc ctttcggaac
     5941 gaggacgact tgggtgattt caaaatacgg gtcgctgaaa tccatagact gtttgcggtc
     6001 gtcggtaatg gttacgcccg acatcacaac gtccgcatcg ccgttgttca aggcggggaa
     6061 aaggctgtcc cacggctggt gtttgaattc gattttaaaa ttgcccgcct tcgccatcgc
     6121 gttcatcaaa tccacgtcga aaccttcgac attgcctttc gagtctaaag attcaaaggg
     6181 ggcaaactcg gcgttggaag ccacgcggta cactttgccg gggttggcgg caggcgcggc
     6241 ggcatctttg ccctggccgc cgcaggcaga cagcgcgagt gcggaacagg caagggcggc
     6301 ggcaatccat tttttcatat tcatcaaaat tcctttcggt aatcaaaaag cctaaaaaat
     6361 cagtttaaca taaaattaac gttccttttc acggaaaaac gcataagtaa cgtcgtggaa
     6421 caaatccgta tgcgggctgt cctcatagct caattctgcc aaggcgtcct caaaagcaag
     6481 ctgaatcgac tcgaccgcga tttcggcggt gtctgcggga agggcgcgga ttaaggcttt
     6541 gaaggcggtg gacaggacgt ggttctgcat acgcagcacg tcgtttgcct cttccagata
     6601 ttccaaacgc tgttcgatgc tgcccatgat gtatcctcgc gaatctgtta aaatgcgcca
     6661 ttatatcaaa ctgcgctttg ggcgcgggtt tgcccgcctg caaagattaa gaaccggaag
     6721 ccgaaaaaat ggatacaaac aatcaggata aagagccgaa ttgggaaaaa cgcctggaaa
     6781 acctgcgtca gacgcgccgt atgaataccg cgcctgccta tgtgccgctg accaaacctt
     6841 ctgaagtcgg aaaggaagag cccataccga aattggacga tgcaaaccga gaccgtgtgt
     6901 taaaggcgta tctgaagaaa tggcaggaag aacacaacag cgccgttgcc gattgcggcg
     6961 aggaagtcga aaccgacccg atgatcgtcc ttcaggaaaa ctggctcaac gcgcaggctt
     7021 ccctgcaaat gccggtttcg gaaaaacaca tcgaatcgcg gcggagaatc cggttcgatg
     7081 ccaaaaaaga cagtgccgaa tttgaaaccg cacagattga gggcggggaa aacgccgatg
     7141 cggcagaatc cggtacggcg gcagacgggg ttgcggaaaa cggcgaaggc ggtgcggaag
     7201 aggcgttgat gcccgtccaa atcaatatcc tgaacccgaa agccgtcaac cggcgcgaag
     7261 tgttctgcct gtccgaacag gagctgaccg aacggttgat caaacgcctg cgcccgcatc
     7321 tgaccgatac cgtcaacggc atgatccgta cggcggtgca gaagcagatg gcgctcttta
     7381 cttatcagct ccagcaaatg ctgcacgagc aggcgggtgc ggtagtcgaa gacgtgttgg
     7441 aacacgatgt ccgtaaaatc ctcaacgaca tcaagtacga attaaaatac aaacgctgat
     7501 tgccatgccg tctgaaagcc gtgttcagac ggcattttcc tgcccgtccg acttgaaagc
     7561 ctcatggaac aggcgcaaaa tattaataaa ttaaacaacg ggtgcacgcc ccgttgccga
     7621 acggacaaaa ccgaggaatc accgatgagc aaaaaacttc atccgcaaac gctcgccata
     7681 cgcggcggca aagaacaaac cggataccgc gaacacaatc aggcattgtt cctgaccagc
     7741 agcttcatgt gggacaacgc ccaacacgct gccgatttgt tttcaaaaaa aatcaaaggc
     7801 ttcacttata cccgtaccgc caacccgacc atcgcggcct ttgaaaaacg catagccgct
     7861 ttggaaggcg cggaacgcgc ggtcgccact tcgaccggca tgtccgcgat tcaggcggcg
     7921 tttttcacct tcctgcaggc gggcggccat gtggtttcca gccgcagcct gttcggcacg
     7981 accgtcggct ttatcaataa catcgttacc aaattcggca tcggagtgag ccgcgtgtcg
     8041 ccgactgata taaatgaatg gaaagccgcc gtcaaagcca acaccaaact gctgtttttg
     8101 gaaacgccgt ccaacccctt gggcgaagtg gccgacttgg aagccttggc ggaattggcg
     8161 cacggcatcg gcgcgctctt ggtggtggac aacagcctgc tgtcgcccgt cggctcgcag
     8221 cctttgaaac acggcgcgga tatttccgtt tcctccgcca ccaaagccat agacggacac
     8281 gggcgcgtga tgggcggcgt gttggcgggt tcggaagaac tgatggcgca ggtcgccgtt
     8341 tactgcaact cttgcggttt ggcgatgtcg ccgtttaacg cgtggcagtt gttgagcggc
     8401 gtggaaaccc tgtcgctgcg tatggaaaaa cagtttgaca acgccttgag aatcgcgcaa
     8461 tggctgcaag agcagccgca ggttcaagca gtgtactaca ccggattgcc cgaccatccg
     8521 caggcggagc ttatccgcaa acagcaaaac ggcggcggca tcgtcatcgg ttttgaagtc
     8581 gccgaccaag cagccgcgtg gaaagtcgtg gacggcgtgg aactcttttc ccgtaccgcc
     8641 aacctcggcg acgtgcgctc gaccatcacc aatccgtgga caaccacgca cggcaggatg
     8701 cagcccgaag aaaaactcgc cgccggtatc cgccccggac tggtgcgcct gtcggtcggt
     8761 ttggaatatg ttggcgattt gattgacgat ttgaaacagg cactcgcgcg ctgattgcgt
     8821 gtttcagacg gcatcggcgg aaaaatgcgg cagatgccgt ctgaaaccct tattgcgtat
     8881 ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg gtttttgccg attgggcgtt
     8941 gaaaatctgc ctgaccggtt tgattatttt tttgggtttt atcgtttgga atttgggcaa
     9001 ggagtccaaa gcgggcaaat tcggcatcgc cgttttgttt ttggttttgg gcttgggtgt
     9061 gttcggtttt gtgttcaaag agttattgat taagtttttg gtattgccca aataggtttg
     9121 ccgtctgtcg aaatgccgtc tgaaatcata aaaagtaatc tgcaccctaa aagtttgatt
     9181 taaccgccaa ctgattaagg tgcgggtttt tatgacaaaa taaaaatcat ctttccgcaa
     9241 tcattcccac ggatgcgaaa gcctgtcttt ttgaatcccg gataatttta ca
//
LOCUS       DAXDZG010000078         9196 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXDZG010000078 DAXDZG010000000
VERSION     DAXDZG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.078"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(152..3004)
                     /gene="gcvP"
                     /locus_tag="ACFB78_001758"
     CDS             complement(152..3004)
                     /gene="gcvP"
                     /locus_tag="ACFB78_001758"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC1269703.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQGCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRSVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3035..3394)
                     /locus_tag="ACFB78_001759"
     CDS             complement(3035..3394)
                     /locus_tag="ACFB78_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269704.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3393..3572
                     /locus_tag="ACFB78_001760"
     CDS             3393..3572
                     /locus_tag="ACFB78_001760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC1269705.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3565..4206)
                     /locus_tag="ACFB78_001761"
     CDS             complement(3565..4206)
                     /locus_tag="ACFB78_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC1269706.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4196..4747)
                     /locus_tag="ACFB78_001762"
     CDS             complement(4196..4747)
                     /locus_tag="ACFB78_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC1269707.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4751..5269)
                     /locus_tag="ACFB78_001763"
     CDS             complement(4751..5269)
                     /locus_tag="ACFB78_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC1269708.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5269..6930)
                     /gene="pqiB"
                     /locus_tag="ACFB78_001764"
     CDS             complement(5269..6930)
                     /gene="pqiB"
                     /locus_tag="ACFB78_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC1269709.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQAKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6923..8257)
                     /locus_tag="ACFB78_001765"
     CDS             complement(6923..8257)
                     /locus_tag="ACFB78_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC1269710.1"
                     /translation="MPPFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     TDVPQLDKGEAAFCPRCGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVVLYFPANILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIAAARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8568..>9196
                     /locus_tag="ACFB78_001766"
     CDS             8568..>9196
                     /locus_tag="ACFB78_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC1269711.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2044 a         2373 c         2623 g         2156 t
ORIGIN      
        1 cccgatttga cgatgcacta ctgtcctatt ttgattttaa ttgactataa agttatagtc
       61 gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct gaaagtgttt
      121 cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc atcggcgggc
      181 agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg aagggccaga
      241 acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag tacggatgcg
      301 cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg ttgtcgtctt
      361 tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc agggcggcga
      421 tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc agcgtgccgg
      481 caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt ttggcgatgt
      541 cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg cattcgtgcg
      601 cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag cgtttagcga
      661 catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt ttgcccatca
      721 tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct gcggcggcaa
      781 cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc ggtgcgaacg
      841 gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg ccgtgaggga
      901 tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc accgacttcg gcgggctgca
      961 tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg ccgttttcgt
     1021 gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg gacggatagg
     1081 taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc aaatcgtcaa
     1141 tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg agcatggcgg
     1201 cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg cggtgtgctt
     1261 cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg ccttgtgcgc
     1321 cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg ctgttttcca
     1381 tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa gggtggatgt
     1441 cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc ttcatggtgc
     1501 agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg agttttttca
     1561 ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt tgcagaatgt
     1621 cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca aatgtgaacg
     1681 tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc gacgtttcat
     1741 ggaatgcagc cgcaacttga gtattgttga cgctgcgcag gttgtaaccc gactccaaag
     1801 cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta acggtatcga
     1861 agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca aagacggaag
     1921 ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga taaacggcgt
     1981 acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat gtcgcttttt
     2041 cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt ttgcccgatg
     2101 cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg tctttaaacg
     2161 cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt tgggtgttgc
     2221 ccaaagcaat atccgcgccc aattcagccg gagatttcag caaaaccaag ctcatgatgt
     2281 cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg tcctgcaagt
     2341 cttgcacgtc gccatcttta ccgacgtatt ggaacagcgc gccgaagtat tcgccttcat
     2401 ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg cgggttttca
     2461 tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg gatttcactt
     2521 tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg tctaacaaag
     2581 acgcgcctgc cacagggaaa ccggtcaaat cgatgcaccc ctgctggaag ttcaacaacg
     2641 cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg taccagcccg
     2701 ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa taacccaagc
     2761 cgatatagga cttgttgatc acgtttttag acgcgatgcc tttcaatttc gccaatgcgt
     2821 ccgcctcggt cagggcttcg ggcaggtcga gttcggacgg catacggatg ctttgcggca
     2881 aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc aacgccgctt
     2941 cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt tcggacagtt
     3001 tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta gacatcggat
     3061 gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc agaatgcagc
     3121 aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg cccaaaacca
     3181 tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa acaacattga
     3241 gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca cgcctgtatg
     3301 ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca gcctgtccgg
     3361 gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca ggccgtcttt
     3421 ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt tttgcacttc
     3481 agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat tatataaaca
     3541 acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa attgattcaa
     3601 attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg atttcagatt
     3661 attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt attcctcgga
     3721 caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca gtgtgatgtg
     3781 cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga tgattttcgc
     3841 gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc cgcctatgcc
     3901 gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata gggcttgcgc
     3961 ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt tttgcagttg
     4021 cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat cgcccaaggc
     4081 aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc tgccgcaggc
     4141 ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg gtttttcaga
     4201 cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga cagctttcgg
     4261 cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt tcgccgccga
     4321 cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc ggatggtgcg
     4381 cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt tgccgtgcat
     4441 tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg gtaagcagcc
     4501 gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct tcaaatgccg
     4561 tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc tgattgattt
     4621 ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg tattgcgtat
     4681 cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca aattcgcaat
     4741 agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt tcgagtgcgg
     4801 cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag ggtctgttcg
     4861 taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag ctgccttgga
     4921 atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca ggaacaaagg
     4981 tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc atatcgtcca
     5041 aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg gtttgataga
     5101 ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt tcgccgcctt
     5161 gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg ctttgcacag
     5221 taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt atcggcttcc
     5281 tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt tttctttcaa
     5341 agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca gcgtattttg
     5401 tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc gcaactcttt
     5461 cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga ccagtttgtc
     5521 aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg cgagcgagcc
     5581 gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca gcaaatccgc
     5641 caatttggcc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg tatcgcctgc
     5701 ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca actcaatcat
     5761 tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt tgtttaaggc
     5821 cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga tttccaaacg
     5881 ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt gcaggctgtc
     5941 attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt tgtattcgac
     6001 aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt agtacagcga
     6061 gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga agctgtcctc
     6121 gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg cgcctgacag
     6181 cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt cgatattgat
     6241 gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg ggctttggat
     6301 gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt cgatttgccc
     6361 gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt cgtttttacc
     6421 aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa tgtcctgcac
     6481 ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaggcgatgt acgaacccga
     6541 aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga ccacccagaa
     6601 ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag taacttccac
     6661 gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga tgctcaatac
     6721 cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga gcgtaaccac
     6781 aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca gcgcgatcag
     6841 cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg cttgagcgtg
     6901 tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa gcaatgccgt
     6961 ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac agcatcgtca
     7021 aaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc gcggcataag
     7081 tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac cagcggccga
     7141 ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc aaagcgaacc
     7201 gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc aaaatactcg
     7261 cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa gcgatgccgc
     7321 taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc ggcaggatat
     7381 tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata ctcagacttt
     7441 tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg gcactgtcgc
     7501 ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga accgcattat
     7561 tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat accgaagtcc
     7621 gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag cgaacctttg
     7681 ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca tccaccatca
     7741 tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga tacgcctgtt
     7801 tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc ggcgcgccga
     7861 aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc atcaggcgca
     7921 tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg atatacgtca
     7981 tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc ggaccggaaa
     8041 aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac gccgcctctc
     8101 ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct accgtatgcg
     8161 gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt tcggcaaacg
     8221 gcttcatcgg gaaagtggca aatataaatg gcggcattat aaaggaaaaa aagcgataca
     8281 cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg gtgtccggca
     8341 aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa aaccccgatt
     8401 ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt ttttcgtaaa
     8461 atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg gcaagtccgg
     8521 attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg agcgaaaccg
     8581 aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg gttcacgaca
     8641 gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac tacatcaaat
     8701 ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa gaccccgagt
     8761 tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta acgcaagact
     8821 tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac gaaaccggca
     8881 acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg gcatttttgt
     8941 tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac ctcgctccgc
     9001 atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat gccctgaact
     9061 taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt gctttctata
     9121 aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc gaaggcatga
     9181 tgccgcacag acatta
//
LOCUS       DAXDZG010000079         9095 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXDZG010000079 DAXDZG010000000
VERSION     DAXDZG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.079"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..377
                     /locus_tag="ACFB78_001767"
     CDS             <1..377
                     /locus_tag="ACFB78_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC1269712.1"
                     /translation="VGGCHAGIAVVCGPFAVIQVIPGENELRQMKPGAVLINCGRGGL
                     ADENALLAALKYGQIGGAGADVLTQEPPRGGNPLLNARLPNLIVTPHTAWASREALDR
                     LFEILLANIHAFVKGEAQNRVV"
     gene            complement(452..2506)
                     /gene="metG"
                     /locus_tag="ACFB78_001768"
     CDS             complement(452..2506)
                     /gene="metG"
                     /locus_tag="ACFB78_001768"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC1269713.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRKTLGGHPINKYEHLMQRMEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(2622..4460)
                     /gene="glmS"
                     /locus_tag="ACFB78_001769"
     CDS             complement(2622..4460)
                     /gene="glmS"
                     /locus_tag="ACFB78_001769"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC1269714.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            4734..5240
                     /locus_tag="ACFB78_001770"
                     /pseudo
     CDS             4734..5240
                     /locus_tag="ACFB78_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5544..6869)
                     /locus_tag="ACFB78_001771"
     CDS             complement(5544..6869)
                     /locus_tag="ACFB78_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC1269715.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPIHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPHKLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7102..7922
                     /gene="efeU"
                     /locus_tag="ACFB78_001772"
                     /pseudo
     CDS             7102..7922
                     /gene="efeU"
                     /locus_tag="ACFB78_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            7919..9052
                     /gene="efeO"
                     /locus_tag="ACFB78_001773"
     CDS             7919..9052
                     /gene="efeO"
                     /locus_tag="ACFB78_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC1269716.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEVKELAAKTKTFT
                     EAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDACEDDFKDGAKDAGFTGF
                     HRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGGASELIEEAAGS
                     KISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILA
                     KYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         1883 a         2267 c         2826 g         2119 t
ORIGIN      
        1 ttgtcggagg atgccacgcc ggcatcgccg ttgtttgcgg gccattcgcc gtgattcagg
       61 taatacccgg cgaaaacgag ttgcggcaga tgaagccggg tgcggttttg atcaattgcg
      121 ggcgcggcgg gctggcggat gaaaacgcgc tgcttgccgc actcaaatac gggcagatcg
      181 gcggggcggg cgcggatgtt ttgacgcaag aaccgccgag gggcggcaat cccttgctga
      241 atgcacgatt acccaatctg attgttacgc cgcataccgc gtgggcgagc cgcgaggctt
      301 tggatcggtt gtttgaaata ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc
      361 aaaaccgcgt ggtttgaacc tgtcgggatt gcggaaaaaa acgccgtctg aacgcccaag
      421 gggttcagac ggcgtttttt gagatttctg cctaaccgac tttgtcgccc ggctgagcgc
      481 ccgcatccac gtcaagcagc ttcagtttgc cgtctgccgt ggcggcggag aggatcatac
      541 cttcggatac gccgaatttt gccattttgc gcggggcgaa gttggcgact gcgatgacca
      601 tgcgaccgtt caattcggca ggatttgggt aagacgcggc gatgccggag aagatgatgc
      661 gtttttcaaa accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct tcgacggctt
      721 cgcagttcaa tactttggcg acgcgcatat cgattttcat aaagtcgtcg aagcccgcct
      781 gttcggcgac tttttcgtat ttaccctctt caacgggcgc ggatgccgtc tgaatgcttt
      841 gtttgttggc ttcgatcaaa tcgtccactt gtttttgctc cattcgttgc attaaatgtt
      901 cgtatttgtt gatggggtgt ccgcccaagg ttttgcgtgt attcgcccag gtaatcgcgt
      961 ccagattgag gaaacgcgcg gcgttggcgg cggtttgcgg caacacgggg gcgaggtagg
     1021 cggtcagcat ggtgaaggcg ttgatgagtt cgctgcatac ttcgtgcagg cgttcgtctt
     1081 gaccttcttg tttggcgagc tcccacggtt tgttggcatc gacgtattcg ttgacaatgt
     1141 ctgccaatgc catgatgtcg cgcagggcgc gggcgtattc gcggttttcg tattgctcgg
     1201 cgatggtgtc gctttcggcg gcgagttttg ccagcaatgc gctgcctgaa acgtctttcg
     1261 gacgaccttc aaagcgtttg gcgatgaaac ccgaagcgcg ggcggcgatg ttgacgtatt
     1321 tgccgacaag gtcgctgttg acgcgcgaaa taaagtcttg caggttcaaa tcgatgtctt
     1381 cgattttgct gttgagcttg gcggcgatgt agtagcgcat ccactcgggg ttcaggcctt
     1441 gttccaaata ggatttggcg gtgatgaagg tgccgcgcga tttggacatt ttttgtccgt
     1501 ccacggtcaa aaagccgtgt gcgtacacgc cggtcggggc gcggtggccg gagaaatgca
     1561 gcatggccgg ccagaacagg gcgtggaaat agaggatgtc tttgccgatg aagtggtaca
     1621 tctcggtttg gctgccggct ttgaagtatt cgtcaaaatc gatgccgatg cggtcgcaca
     1681 ggtttttaaa cgatgccatg tagccgacgg gcgcgtcgag ccagacgtag aagtatttgc
     1741 ccggcgcgtc ggggatttca aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg
     1801 acagggtggt ttctccgcct tcgcccagcc attccttcat tttgttgagg gcttcgggtt
     1861 gcagatgggg cttgccgtcg tgcgggttgt tgccggaagt ccatgctttg aggaagtcgg
     1921 cgcattcgcc cagtttgaag aagaagtgtt cggattcgcg caattcgggt ttcgcgccgg
     1981 aaacggcgga atacgggttg aacagttcgg tcggggaata ggtcgtgccg cagacttcgc
     2041 agttgtcgcc gtattggtct tgggcgtggc atttcgggca ttcgcctttg acgaagcggt
     2101 cgggcaggaa catttgtttt tcggggtcga aaagctgctc gatgacgcgg ctttcgattt
     2161 tgccgttggc tttcagcgcg cggtaaatgt cttgggaaaa ctgtttgttt tcagtggaat
     2221 gggtgctgta ataattgtcg tagccgatga aaaagccggt aaagtcggca aggtgctctt
     2281 tgcgcacttt ggcaatcatg tcttcgggcg cgatgccttg tttttgcgcg gcaagcataa
     2341 cgggcgtgcc gtgggtgtcg tcggcgcagc agtagtagca ttcgtggccg cgcagttttt
     2401 gaaagcgcac ccaaacgtcg gtttggatgt gttcgaccat gtggccgagg tggatgctgc
     2461 cgttggcata gggcagggcg gaggtaacta agattttgcg tgtcatattg tgctttgcaa
     2521 acaatgggta aaggcggatt ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga
     2581 aaaatcgggc ttcagacggc atttttgcca accggcggga tttattcgac ggttacggat
     2641 ttcgccaggt tgcgcggctt gtccacatcc gtgccgcgcg cgagggcggt gtggtaggcg
     2701 aggagctgca cggggacggt atgcacgacg ggggacagtt cgccgacgtg gcgaggtgcg
     2761 cggataacgt gcacgccttc ggcggcatta aaattgctgt cgaggtcggc aaagacgaaa
     2821 agttcgccgc cgcgcgcgcc gacttcctgc atattggctt tgactttgtc caacaggctg
     2881 tcgttgggcg cgatgacgac gacgggcatg ttttcgtcca ccaaggcaag cggcccgtgt
     2941 ttcagttcgc cggcaggata ggcttcggcg tggatgtagg tgatttcctt tagcttcaac
     3001 gcaccttcaa gggcaatcgg gtaatggatg ccgcgcccca aaaacagcgc gctggttttc
     3061 ttggcaaact gttgcgccca agcggcaatt tgcggttcga ggttcaaagc gtgctgcacg
     3121 ctgccgggaa gctggcggag ttcttcggtg taacgtgctt catcttcgcc ggataccacg
     3181 ccgcgcactt tcgccagcgt taccgccaaa ccgaacagca cgaccagttg cgtggtaaat
     3241 gctttagtcg aggcgacgcc gatttccgca ccggcacggg tataaagcac aaggctgctt
     3301 tcgcgcggca gggcggattc catcacgttg cagacggaga ggctgtggcg gtgtcccaaa
     3361 gatttggcgt atttcaaagc ctccatcgtg tccagcgttt cgccggattg ggaaatggta
     3421 atgaccagtt ggtcggggtc ggcaatcacg ctgcggtacc ggtattcgct tgcgatttcg
     3481 acgtcggccg ggattttggc gatggattcc aaccaatatt tggcggtcag cgcggcgtaa
     3541 taggacgtgc cgcaggcaag gattttgacg ctgcggatgc tttcaaacac gcttttggca
     3601 tttttgccga agttttcggg gatgaatccg ccgtcgagga aaacttccgc cgtgtccgca
     3661 atcgcgcgcg gctgctcgtg gatttctttt tgcataaagt ggctgtgcgg acccggttcc
     3721 aaagaggcgg gcgagagttc ggataccttg actttgcgtt cggcaggcag gccgttttta
     3781 tcggtcagcc gtttgatgcc gtctgaagcc agcagcgcga tgtcgccgtc ttcgaggtac
     3841 gccacgcggc gcgtaaaggc gatgacggcg gatacgtccg aagcgataaa ggtttcatcg
     3901 tcgcccaaag cgaccaaaag cgggcagccc atacgcgcca caactaattc atcaggcttg
     3961 tcttgggcaa taaccgcgat ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcacg
     4021 gcttcaaaca atttgccgcc gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact
     4081 tcggtatccg tttgcgattc aaaccggtat cccaaacctt ccaagcgttt gcgttcgctt
     4141 tcaaagtttt cgatgatgcc gttgtggacg acggcaatca taccgccgct gatgtgcggg
     4201 tgggcgttcg gctcggtaac gccgccgtgt gtcgcccagc gcgtatgccc gatgccgatg
     4261 ccgccgctga tgcctttttc gcgtgccgcg tcctccataa gctgcacgcg tccgacgcgg
     4321 cgcacgcgct tgattttgcc gtctgaatac acagcaatgc ctgatgagtc ataaccccgg
     4381 tattcgaggc gtttgaggcc gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg
     4441 gcgccgacga taccgcacat aaactgttcc ttagtatccg gttgaaaaaa aagatgcgga
     4501 cggcttccgt accgcacctt ccgtttcgga ttataaaccg cctcccgcgc cggaaaacag
     4561 caaaatgccg tctgaaggct tgggcttgct caaaaaaggg agggatttcc ccgcctgacc
     4621 gggatgggcg ttcagacggc attgcctgcc gccggtttat agtttttgca aatcaatatt
     4681 aacaagctga aaaaaaaaac aatatacccg cccggtctta atgttaacgg agtatggaaa
     4741 tgaaacaaat gcttttggcc gtcggcgtgg cggcggtgtt ggcgggctgc ggcaaggatg
     4801 ccggcggtta cgagggttat tggcgcgaaa agtcggacaa aaaagagggc gtgattgccg
     4861 tcaaaaaaga aaaaggcaat tacttcctta ataaaatcaa cgtgtttaca ggcaaggagg
     4921 agtctttgct tttgtctgaa aaagacggcg cgctttcgat aaacacgggg ataggggaaa
     4981 tcccgatcaa actttccgac gacgggaaag agctgtatgt cgaacgcagg cggtatgtga
     5041 aaaccgatgc ggcgatgaag gacaaaatca tcgcccacca gaaaaagtgc ggacaaacgg
     5101 cacaggcata cctcgacgcg cgaaatgcgt tgccgtcaaa ccaaacgtat cagcagcgtc
     5161 aggcggcgat cgagcaattg aaacggcggt ttgaagccga gtttgacgaa ttggaaaaag
     5221 aaatcaaatg caacggcaga accccggcat tgttgctttg gcaggggaca accgggagga
     5281 tgccgccgtc cgaatcggac gcgtggtttc cataccggta cgggcgggcg ggaatgtccg
     5341 cctttttttg ttcggatgcg tttgaatacc cgtccgattc tgtccgtttg caagggggat
     5401 ttccgttcgg gcggaaatcc attccgtcaa ataagaaagg cgttttgtcc gaccggccgg
     5461 cttgtgcctg cggtatcgca aaacttcgcc ttaatgcgcc cgattggcta gggacgggct
     5521 tcggacggca ttgttttgcc ggttcacggg cggtattcgg gcttcatgcc gttgggcagg
     5581 agctgccaga cgtatcctgt ggttttctgt ttgccggcaa gttcgccggc ttcgtcgccg
     5641 taaccccaaa aataatccac gcgcaccgcg cctttgatcg cgctgcctgt atcctgcgcc
     5701 ataatcaggc ggttgagggc tttgcgggta accggatggg cggtggcgac aaataagggc
     5761 gcgcccaagg taatgtagtg ccggtcgatt gcgccggcgt attcccccat cagcggcgtg
     5821 cccagtgcgc cgacggggcc ctcattgccg cttccggcaa gctcgcggaa aaagatatag
     5881 ctggggtttt gacccaaaac ttcggcgagt ttgtgcgggt tttgccgcat atatgactta
     5941 atgccctgca tggaggtttg cccgagcttg aggtagcctt tgtccgccat atagcgtccg
     6001 atggaaacat acggatgctc gtttttgtcg gcatagccga tgcggatgta tttgccggac
     6061 ggggttttca gacggcccga gccttgtatg tgcatgaaaa aaagttcgac ggggtcttcg
     6121 gcgtaaccga gtatcggggc tttgccgtca agcgcgccgc cgttgatttg gttgcgcgtg
     6181 tggtagggga ggaagcggct tccttcaaac ctgcccttga ttgccgttgt gcgcgcggta
     6241 atggggaatt gggaaaggtc ggcggtatgc gtgccgccgg cattgtcgat tgtgccgctg
     6301 ttttttcccg tctgcctgat gcggacaagg gctttcccgc cccgcaaacc ggcaggcagg
     6361 gggacggaga taaaatcgtc gggaatgccg taaatcggga agcgggcccg ttccgtccgc
     6421 ctgccgtcgc ccttcagcac cggttcgtaa tagccggtaa ctgtgccggc aaggcttccg
     6481 ttgcctgcaa cctgccaagg cgtgaaatag cgttcaaaaa accgctttgc ctgaaaggaa
     6541 tggatggggg tttgaaaggc ttgggcgcac acatcctgcc agccttggcg gtttttcaaa
     6601 ttggcgcagc cgaggcggaa ggattgcagg cttttggcaa aatcctgcgc cgcccagtgg
     6661 ggcatggaca ggtgcggcac aacggtatag acggccccgc cgccggcaac cgtcgttccg
     6721 gcggggtcgg ggatgccggc cggccggtcc gggccgttga tgacggatgt gtcgggttgc
     6781 ggaaaggttt ggatgctcct gctttggcag gcggcgagga tggcggcggc gatgccgtac
     6841 agggcggagc ggagcaggtg ttttttcata atggacaatg ttgtcgtcag taataaaaaa
     6901 agatggtttc gggcggcgtt gcggtagccg gggagagggg attttaacac agggcgcagc
     6961 cgcagcctgc ggaactttcc gccacgcggc aatgcagata aaaataactt gcatttgtat
     7021 ttgcaagtaa tgaaaatatt ccgataatat attattaatc atccttgttc attcgcgttt
     7081 acgctggtcg cttttttaat tatgttgcgc gagggtatcg aagccgcgct catcgtcggc
     7141 atcgttgccg gttttttgaa acagtccgga cattcaaaaa ctgatgccta aggtctggtt
     7201 cggggtcgtc cttgcttctt tgatgtgttt gggcatcgga tacggcatcc attcggcaac
     7261 gggcgagatt ccccggaagc agcaggagtt cgtcgtcggc attatcggtt tggttgccgt
     7321 cgcgatgctg acttgtgtgg ttttatggat gaaaaaggcg gcgcgttcga tgaagcgcca
     7381 gcttcgggat tctgtacagg cggctttgaa ccgtggcagc ggtcaaggat gggccttggt
     7441 cggtatggcg tttcttgccg tggcgcgcga aggtttggag agcgtttttt ttcctgcttg
     7501 ccgtattcaa acagagcccg acgtggcaga tgccggccgg cgcggtagcg ggggttttgg
     7561 ctgccgccgt gattggcgcg ttgatttatc agggcgggat gcgcctgaat ctggcgaagt
     7621 ttttccgttg gacgggggcg tttctgatta tcgttgccgc cggtctgctt gccggctcgc
     7681 tgcgtgcgct gcatgaggca ggcatttgga acgcgcttca ggacattgtg ttcgacccat
     7741 caaaatattt gcacgaagac agtccgttgg gtgtgctgct cggcggattt ttcggctata
     7801 ccgaccatcc ggcgcagggc ggggccttgg tttggctgct gtaccttatt cccgtcatga
     7861 tttggttttt gcgcggcagc aggccgtctg aaactttaac ccgtaaagag gaattgaaat
     7921 gagaaaattc aatttgaccg cattgtccgt gatgcttgcc ttgggtttga ccgcgtgcca
     7981 gccgccggag gcggagaaag ccgcgccggc cgcgtccggt gagacccaat ccgccaacga
     8041 aggcggttcg gtcggtatcg ccgtcaacga caatgcctgc gaaccgatga atctgaccgt
     8101 gccgagcgga caggttgtgt tcaatattaa aaacaacagc ggccgcaagc tcgaatggga
     8161 aatcctgaag ggcgtgatgg tggtggacga acgcgaaaat atcgccccgg ggctttccga
     8221 caaaatgacc tgcggccttt tgaccaatcc gcgcggcaag ctggtggtag ccgacagcgg
     8281 ctttaaagac accgccaacg aagcggattt ggaaaaactg ccccaaccgc tcgccgacta
     8341 taaagcctac gttcaaggcg aggttaaaga gctggcggcg aaaaccaaaa cctttaccga
     8401 agccgtcaaa gcaggcgaca ttgaaaaggc gaaatccctg tttgccgaca cccgcgtcca
     8461 ttacgaacgc atcgaaccga ttgccgagct tttcagcgaa ctcgaccccg tcatcgatgc
     8521 gtgtgaagac gacttcaaag acggtgcgaa agatgccggg tttaccggct tccaccgtat
     8581 cgaacacgcc ctttgggtgg aaaaagacgt atccggcgtg aaggaaaccg cggccaaact
     8641 gatgaccgat gtcgaagccc tgcaaaaaga aatcgacgca ttggcgttcc ctccgggcaa
     8701 agtggtcggc ggcgcgtccg aactgattga agaagcggcg ggcagtaaaa tcagcggcga
     8761 agaagaccgt tacagccaca ccgatttgag cgacttccaa gctaatgcgg acggatctaa
     8821 aaaaatcgtc gatttgttcc gtccgttgat tgaggccaaa aacaaagcct tgttggaaaa
     8881 aaccgatacc aacttcaaac aggtcaacga aattctggcg aaataccgca ccaaagacgg
     8941 ttttgaaacc tacgacaagc tgagcgaagc cgaccgcaaa gcattacagg ctcctattaa
     9001 cgcgcttgcc gaagaccttg cccaacttcg cggcatactc ggcttgaaat aagccgcaag
     9061 cgttcagacg gcatttggcg gcagataccg tctga
//
LOCUS       DAXDZG010000080         8637 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXDZG010000080 DAXDZG010000000
VERSION     DAXDZG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.080"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..258)
                     /locus_tag="ACFB78_001774"
     CDS             complement(<1..258)
                     /locus_tag="ACFB78_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269717.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB78_001775"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB78_001775"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC1269718.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACFB78_001776"
     CDS             3148..4278
                     /locus_tag="ACFB78_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1269719.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACFB78_001777"
     CDS             complement(4319..5221)
                     /locus_tag="ACFB78_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC1269720.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="ACFB78_001778"
     CDS             5360..5857
                     /locus_tag="ACFB78_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC1269721.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB78_001779"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB78_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC1269722.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="ACFB78_001780"
     CDS             6953..7288
                     /locus_tag="ACFB78_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC1269723.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7419..7889
                     /locus_tag="ACFB78_001781"
     CDS             7419..7889
                     /locus_tag="ACFB78_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269724.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB78_001782"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB78_001782"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC1269725.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVAAVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT         2037 a         2137 c         2304 g         2159 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggctaa aacggcttcg ttgacgcggc gttcgacttc agcgatttct tcggcagtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaacg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagtggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggcaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaac acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggtat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatt ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccgccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcg
//
LOCUS       DAXDZG010000081         7984 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXDZG010000081 DAXDZG010000000
VERSION     DAXDZG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.081"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            8..221
                     /locus_tag="ACFB78_001783"
                     /pseudo
     CDS             8..221
                     /locus_tag="ACFB78_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            331..1008
                     /locus_tag="ACFB78_001784"
     CDS             331..1008
                     /locus_tag="ACFB78_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC1269726.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1282..2052)
                     /gene="xth"
                     /locus_tag="ACFB78_001785"
     CDS             complement(1282..2052)
                     /gene="xth"
                     /locus_tag="ACFB78_001785"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC1269727.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2112..2393)
                     /locus_tag="ACFB78_001786"
     CDS             complement(2112..2393)
                     /locus_tag="ACFB78_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC1269728.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2450..2716
                     /locus_tag="ACFB78_001787"
     CDS             2450..2716
                     /locus_tag="ACFB78_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269729.1"
                     /translation="MILIIFINCIINQLFISHCTGLYVPFPTIFANKRKDRLSDGIKL
                     PPPSFPPVLPPAGIIRIVQKMPYNRRTPCPADASAPSGKQNIVD"
     gene            complement(3017..3898)
                     /gene="nadC"
                     /locus_tag="ACFB78_001788"
     CDS             complement(3017..3898)
                     /gene="nadC"
                     /locus_tag="ACFB78_001788"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC1269730.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3974..4912)
                     /locus_tag="ACFB78_001789"
     CDS             complement(3974..4912)
                     /locus_tag="ACFB78_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269731.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5114..6226
                     /gene="nadA"
                     /locus_tag="ACFB78_001790"
     CDS             5114..6226
                     /gene="nadA"
                     /locus_tag="ACFB78_001790"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC1269732.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKVLLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6278..7786
                     /gene="nadB"
                     /locus_tag="ACFB78_001791"
     CDS             6278..7786
                     /gene="nadB"
                     /locus_tag="ACFB78_001791"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC1269733.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRISHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1741 a         2357 c         2239 g         1647 t
ORIGIN      
        1 ctaaacccac catccgcgcc tgccgcacgt acacgtcgac aaaccgaaaa tatggctgct
       61 cggcggcatc gtcggcgcgt ggggctgcca taaaatcgga caccatttct ccctgcccgt
      121 ttcggcatta ttgccgatac cgggtgcagg ctcggtcggc tacgatgtga aggtacgttt
      181 ttttgcggat gtggctgcgg tttcggcgca aatcacgata aacccgcgtt gtttcgggaa
      241 aaatcgggta atttcaggtt aattgcactt tttcagacgg catgattgct tatagttgta
      301 cggtttgaca tatgcacgga aggaaacgcc atgactttct tcaaaccctt taccgtcgtg
      361 ctgaccgcat ccgcactcgc gctttccggc tgcgttgccg accccgtaac cggacagcag
      421 tccccaaaca aatccgccat gtacggtttg ggtggcgcgg cagtgtgcgg catcgtcggc
      481 gcactgaccc acagcggcaa aggcgcacgc aattccgcgc ttgcctgcgg cgcaatcggc
      541 gcaggcgtgg gcggctatat ggactaccaa gagcagcgtt tgcgccaaaa ccttgccggc
      601 acgcaaatcg aaatccaacg ccaaggcaac caaatcaggc tggtgatgcc cgaaagcgtt
      661 accttcgcca ccggcagcgc ggcgttgggc ggcagtgcgc aatacgccct gaacactgcc
      721 gcacagacgc tggtgcagta tcccgacacg acgctgacca tcaacgggca caccgacaac
      781 acaggttccg atgcagtcaa caatccgctt tcgcaacacc gcgcccaagc ggttgcctac
      841 tatctgcaga cgcgcggcgt ggcggcttcg cgcctgacgg tttacggcta cggttcgcat
      901 atgccggtcg cgtccaacgc tacggttgaa ggccgcgcgc aaaaccgccg cgtcgaaatc
      961 ctcatcaacc ccgaccaacg cgccgtcaac gccgcacggc acatgtaacg cgccctgcaa
     1021 cgccaatgcc gtctgaatgt ttttcagacg gcatttccgt attcgcaagc acagccgtac
     1081 cccgactgta atgctatgga taacgtctcg ccgtttgccg ggaaaccgca gattgcagcc
     1141 ggtggcgtgc aagtgggccc aaccttgcag acgttaccgc cataccgcga tacccctgta
     1201 acctcatcgt ttaaacagat attcaatcaa aatgccgtct gaaaccctgc gaagtttcag
     1261 acggcatttg cctttcccga attacaaatc aaattctgcc gccacgggcg cgtggtcgct
     1321 cggacgctcc agcgcgcgcg tctccaaatc gatgcggaca tccttcaaaa cggccgccat
     1381 ttcggatgta accaatatat ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc
     1441 gcgatagtcg aaccaggtat agaacgcgcc ttcggggtgg acttggcgca ggctgtcggt
     1501 caatcccaaa tccagcagat tttgaaacca ctgccgttcg acggacgaac agtggatttt
     1561 ttcataccat ttttcagggt cgtaacagtc cgcatcggca ggcgcaatat tgaaatcgcc
     1621 cagcagtacc agtttgccgt ggcgggtcat ttcgtcgcgg acaaactccg tcagtgcggc
     1681 aaaccactgt tccttatatt tgaatttggg gctgtccaaa gcctcgccgt tgacgcaata
     1741 gacattgatg acgcgcacgc cgcctacggt cgccgcaatc acgcggcgtt gcgggtcatc
     1801 cggcagggaa ggcaaaccga aatgcacgtc ctgcggcacg ctgcggctga cgattgccac
     1861 accgttgtag gttttctgcc cgctccaaac acagtgccag cccatcatct gcaaggcggc
     1921 ggccggaaat ttgtcctgat cgagtttgag ttcctgcaaa accaaaatat cgggcggatt
     1981 gtcggcaagc aggttttgca cctgcggcag ccgcacattg agcgaattga cgttccaagt
     2041 ggtgattttc ataacatttc cgaaaaatgc cgtctgaaac gttcagacgg caaaagaaaa
     2101 ggattctaac actatgccgc gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt
     2161 gccgcgtctt cggaaaccag gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc
     2221 agaccgcccg ccctcaggcg gttcaaatga caggaaaccg cggtagccga cagggataag
     2281 gattttgcca gttcgacgat attgcgttcg ctgtccaaca gttggatcag tatcgccata
     2341 cgttcgggat gcgccataag tttgagtatg gtgtggagcg gtattgtgtt catcggtatt
     2401 cctaaaacct tttccgcccg ccaagcggat tgacgggcgg cagatatgga tgattttgat
     2461 tatctttata aattgtatta taaatcaatt gtttatttct cattgtacag gattgtatgt
     2521 tccttttccc actatttttg caaacaagcg taaagacagg ctttcagacg gcataaaact
     2581 gccgcccccg tcctttcccc ctgtcctgcc gccggccgga atcatccgca ttgttcaaaa
     2641 gatgccttac aaccgccgca cgccttgccc tgccgatgcc tccgcgcctt cgggcaaaca
     2701 aaatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtataaatag
     2761 tacggaaccg gttcacttga tgcttcagca ccttagagaa tcgttctctt tgagccgggg
     2821 cggggcaacg ccgtaccggt ttttgttaat ccactatacc tgcctgaaat gctgtttttt
     2881 ctaaagcatc acgaccttca cgaaaaatgc cgtctgaaga atatttcaga cggcattccc
     2941 tccctgaggc aataaccgga ttacggttaa gcggttcctt acctgacata tcagacagcc
     3001 gcccgcaccc tgaaactcac gccacgaaat ccaaacctat gtccaacgat cggctgctgt
     3061 gggtcagata gccgagggcg atgccgtcca cgccggtttg cgccacgcgc ttcaggcggt
     3121 cgaagccgat gccgcccgat gcttcgcaat agacggtgtg ggggtgggcg gtttgtgtgt
     3181 ggcagcggtt tgccgcttct ttcagggttt cgtcgtccat gttgtccagc aaaatccgtt
     3241 ccgcgcccgc tgcgatggct tcgtccagtt gtgccaatgt gtccacttcg atttccacgc
     3301 aggtcaatgg tccgaccgcc tgttttgcct gccgaacggc ttgggcgatg ctgccgcaat
     3361 aggcgaggtg gttgtctttg atgagcacgg cgtcgtccaa acccatgcgg tggttcgcgc
     3421 cgccgcccgc cctgacggcg tatttttgca ggacgcgcag cagggggatg gttttgcggc
     3481 tgcacacgat gtctgtaccg tattcggcga cctcggcaac ggcacgagcg gtggcggtgg
     3541 cgatgccgct taagtgcgtg aggtagttga gcgcggtgcg ttcggcggcg agcagcgcgc
     3601 gggcgttgcc ttcgacggca gccagtgtct gacctgcgcg gacggcttgc ccgtcttgga
     3661 tttcggcttg gaagcggacg cacggatcca tcgtctgaaa ggcgaggcgc gccaagtcca
     3721 taccggcgat aacaccgtct tcgcggctga cgaggaaaag tttggcggtt ttgtcgggcg
     3781 cgatgacggc ggcggacgta atgtcgccgc gcctgcccaa gtcttcgctc aaggcttgtt
     3841 ccaccatagg gcgcaacaag gtgtcgggca gggcaaagag ggggttttca gacggcatgg
     3901 ggtttccttt gttaaaaaag attgatgttg caccgatggg gcaaaacttc atttgatgcc
     3961 gtctgaaaac gggctaacgg ctgcccagcg gcagtccgat gtccgaaatc agcctgtcgg
     4021 gcaggacgtc gtcgcggaag cggcaaagct gcgcgggacg gcctttgctg cccgatacgc
     4081 ctgtatccga cggctcgatg aggttttgct gctgaatctg gcggcggaag ttttgcttgt
     4141 gcagcaatct gccgctgatg gcttcgacgc tgttttgcag ttgcagcagc gtgaattcgg
     4201 gcggcatcag ttcgaaaatc acgggacggt atttgatttt ggcgcgcagg cgggacaggg
     4261 cggtcgccaa cacgcggcgg tggtcgtggc gcatggtctg ccccgtgagc gcgaagtcga
     4321 agtttgcctg cggctcggcg gcctccgcta tcagaccgct ttcatacaac atttcatagc
     4381 gttgcaaaac gtattcttcc gaccagtttt ccggttcgac cccccaacac aaatgaatat
     4441 gcttgagccg cttttggcgg acttcctccg tgtccgccga gtttgcccaa atgcgcaggc
     4501 ggctgacgac ggcgtcgcgc tgcccgccgt cggtgcgcaa gtcttcccat gggaaatagc
     4561 cgtagcagtc ctgccatttc gcgtcgggat gcaggatgct atcggctgcc tcgcgcacca
     4621 gccccaaata gctgacgtac agcacgggca tgccgtgttc gttgcggcgg tgggtatcga
     4681 caaaggtgta aagctgttcc acatagccca taggctgcga agtctgcttg gcgacccaca
     4741 gctttacgcc cgcctgcagg gaattgcgca ggggggaaag cgggccgttg ggcaggagcg
     4801 tgccttgggc gacggtcaac acccgcaggc cgccgtcggt aacggcaatc aatacgggaa
     4861 ccagctcgac gatgctttgc agcggagctt cggcttcggg gtaggcgtcc atgctcggct
     4921 ttctgcgggt gggaaaggct ttattgtaaa ccaaagcggt ttgtcggaac gaagcgggtc
     4981 ggttttgcgg tatcgcaaac ctgttttcag acgaccttgc atattatact caagttgagc
     5041 ataatgtaaa aacagtttga aaaacagacg gcaaaccttc atgccgtctg aaaaccagct
     5101 acaaggaaac atcatgcaaa ccgccgcccg ccgctcgttc gattacgata tgcccctcat
     5161 ccaaacgccg acttccgcct gccaaatccg tcaggcgtgg gcgaaggttg ccgacacgcc
     5221 cgaccacgag acggcaggtc gtctgaaaga cgaaatcaag gtcttgctga agcggaaaaa
     5281 cgcggtcttg gtggcgcatt attacgtcga tccgctgatt caggatttgg ctttggagac
     5341 gggcggatgc gtgggcgatt cgctggaaat ggcgcgcttc ggcgcggaac acgaagccgg
     5401 tacgctggtg gtggcgggcg tgcgtttcat gggcgagagc gcgaaaatcc tctgccctga
     5461 aaaaacggtg ctgatgcctg atttggaagc ggaatgttct ttggatttgg gttgtcccga
     5521 agaagcgttt tcagcgtttt gcgaccaaca ccccgaccgc acggtggcgg tgtacgccaa
     5581 tacttccgca gccgtgaaag cgcgtgccga ctgggtggta acgtcttcgg tggcgttgga
     5641 aatcgtgtcg tatctgaaat cgcgcggcga gaaactgatt tgggggcccg accgccatct
     5701 cggcgactac atccgccgcg aaacgggcgc ggatatgctg ttgtggcagg gttcgtgcat
     5761 cgtccataac gaattcaaag ggcaggaact ggcggcgttg aaggcagagc atcccgatgc
     5821 tgtggtgctg gttcatcccg aatcgccgca aagcgtcatc gaactgggcg acgtggtcgg
     5881 ctcgaccagc aaactactca aagccgccgt atcgcgccct gaaaaaaaat tcatcgtggc
     5941 aaccgatttg ggcatcctgc acgaaatgca aaagcaggcg cccgacaaag aatttatcgc
     6001 cgcgccgacg gcgggcaacg gcggaagctg taaaagctgc gcgttctgcc cgtggatggc
     6061 gatgaattcg ctgggcggca tcaaacacgc cctgacaggc ggacgcaacg aaatcctgtt
     6121 ggacagaaag ctgggcgaag ccgccaaact gcctttgcag cgtatgctcg acttcgcggc
     6181 aggactcaaa cgcggggatg tgttcaacgg catggggccc gcctgatttg ccgtccatat
     6241 cgccgtttca gacgacctct caaacaagga caacaccatg caaaccgatt gcgacgtatt
     6301 gattgccgga aacgggctgg cggcactgac gctcgccctg tcgctgcctg aatcgttccg
     6361 catcgtcatt ttgtgcaaaa accggctgga cgacaccgcc agccgtcatg cgcaaggcgg
     6421 gattgcggcg gcgtggtcgg gagaggacga catcggaaaa cacgtcgccg atactttgga
     6481 agcgggcgca ggtttgtgcg acgaagccgc cgtccgcacc atcctgtcgc agggcaaacc
     6541 ggcaatcgaa tggctgctgg cgcagggcgt ggcgttcgac cggaatcata acgacctgca
     6601 cctgacgcgc gaaggcgggc atacctgccg ccgaatctcc cacgtcgccg actacacggg
     6661 cgaagccgtc atgcagagcc tgattatcca aatacgccgc cgaccgaaca tccgcgttta
     6721 cgagcggcag atggcgttgg acgttcaaac cgaatcaggc gcagcgtgcg gactgaccgt
     6781 cctcgactgc cgaacgcaag aaacctaccg catccgtgcc cgccataccg tactcgcagg
     6841 cggcggcctc gggcaaattt acgccgccac caccacgccg cccgaatgca cgggcgacgc
     6901 catcgccatg gcgatacgcg cgggttgcgc aatcgaaaac ctcgaattta tccaattcca
     6961 ccccacaggc ttggcaaggc cgtctgaaaa cggccgcacc ttcctgattt ccgaagccgt
     7021 gcgcggcgaa ggcggcatcc tgaccaatca atcgggcgaa cggtttatgc cgcattacga
     7081 ccgccgcgcc gaactcgcgc cgcgcgacat cgttgcccgc gccatcgccg ccgaaaccgc
     7141 caaacaaacg caagacttcg tctcgctcga catcagccgc caacccgcag cgttcgtccg
     7201 tcggcatttc ccgtccatcc atcggcactg cctgtcccaa tgcggcttgg acatcacgcg
     7261 ccaagccatc cccgtccgcc ccgtgcaaca ctacacctgc ggcggcatcc aaaccgaccc
     7321 cagcggcaga acctccctgc cgcagctcta cgccttaggc gaaaccgcct gtaccggact
     7381 gcacggagcc aaccgcctcg ccagcaactc cctgctcgaa tgcgtcgtta ccgccaggct
     7441 tgccgcccaa ggcatcgcgg acggacaagc gttccaaatc gaaccattcc aaaggccgtc
     7501 tgaaagcccc tccgccgaaa cagacatctt ttcaaacgac ctccaaaaca cattcagccg
     7561 ccctgtcctg caaacgttca accaacgcca tctcggcatc ctccgcaacg ataccgacct
     7621 gcaccgcgcc atcgaccaac tgcggctttg gaagcaaaac caagccgaac cgcacaccgc
     7681 gtccgaatac gaaaaccgca acctactcga atgcagcctc gccgtcgccc aagccgcata
     7741 caggcggaga cagaacatcg gtgcgcattt taatagtgat tgttaaggtt ggctggttag
     7801 aaaaaaatcg ttcaaataaa tttgaatagc atggttaaga gattggcatt cagtttttat
     7861 ggttgcgtaa gttgggttga ggcctttgca aaaaagccct tccttcgaca gccgaaaccc
     7921 aaacacaggt tttcggctgt tttcgcccca aatacctcct aattttaccc gaatatcccc
     7981 ttaa
//
LOCUS       DAXDZG010000082         7644 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXDZG010000082 DAXDZG010000000
VERSION     DAXDZG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.082"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            76..1241
                     /gene="prpF"
                     /locus_tag="ACFB78_001792"
                     /pseudo
     CDS             76..1241
                     /gene="prpF"
                     /locus_tag="ACFB78_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            1553..2752
                     /locus_tag="ACFB78_001793"
     CDS             1553..2752
                     /locus_tag="ACFB78_001793"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC1269734.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2863..3483
                     /locus_tag="ACFB78_001794"
     CDS             2863..3483
                     /locus_tag="ACFB78_001794"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC1269735.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(3507..3635)
                     /locus_tag="ACFB78_001795"
     CDS             complement(3507..3635)
                     /locus_tag="ACFB78_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269736.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(3645..4088)
                     /locus_tag="ACFB78_001796"
     CDS             complement(3645..4088)
                     /locus_tag="ACFB78_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC1269737.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            4262..4630
                     /locus_tag="ACFB78_001797"
     CDS             4262..4630
                     /locus_tag="ACFB78_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC1269738.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            4675..5829
                     /locus_tag="ACFB78_001798"
     CDS             4675..5829
                     /locus_tag="ACFB78_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC1269739.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5887..6759)
                     /locus_tag="ACFB78_001799"
     CDS             complement(5887..6759)
                     /locus_tag="ACFB78_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC1269740.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6844..>7644
                     /locus_tag="ACFB78_001800"
     CDS             6844..>7644
                     /locus_tag="ACFB78_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC1269741.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVL"
BASE COUNT         1628 a         2175 c         2215 g         1626 t
ORIGIN      
        1 caacccgtac cggtttaaat taatccacta taaaaatcgt gggcagagcc cacgctacat
       61 aaggagaacc ctaaaatgcc gcaaatcaaa atccccgccg tttactaccg cggcggtaca
      121 tctaaaggca ttttcttcaa acgcaccgat ctgccggcag ctgcgcagga agcaggcggg
      181 gcacgcgaca aaatcctctt gcgcgtactc ggcagccccg acccttacgg caaacagatt
      241 gacggtttgg gcaacgccag ttcgtccacc agcaaagctg tgatttcgga caaatccgaa
      301 cgcgccgatc acgatgtcga ttacttgttc ggacaagttt ccatcgacaa accatttgtc
      361 gattggagcg gcaactgcgg caaccttacc gccgccgtgg gcacattcgc catcgaacag
      421 ggcttggtcg ataaatccaa aatcccttca gacggcatct gcacggtcaa aatctggcag
      481 aaaaacatcg gcaaaaccat tattgcccat gtgccgatgc aaaacggagc agtgttggaa
      541 acaggcgatt tcgagcttga cggcgtaatt ttccggcagc cgaagtacaa atcgaatttt
      601 tagatccagc cgacggcgaa ggcagtatgt tcccaaccgg caatttggtc gacgaaattg
      661 atgtgccgaa tataggtcgt ctgaaagcca cgctcatcaa cgcgggcatt ccgaccgttt
      721 tcctgaacgc cgccgacttg ggctacacgg gcaaagagtt gcaggacgac atcaacaacg
      781 atgccgccgc gctggaaaaa tttgaaacca tccgcgcata tggcgcgctg aaaatgggtt
      841 tgatcagcga cgtatccgaa gccgccgccc gcgcgcgcac gccgaaaccc gccttcgtcg
      901 cgcccgccgc cgattacacc gcctccagcg gcaaaaccgt aaacgccgcc gacatcgatt
      961 tgccggtacg cgccctgagc atgggcaaac tgcaccacgc tatgatgggc atcgcctcgg
     1021 tcgccatcgc cgccgccgcc gtgctcggta cgctggtcaa ccttgccgca ggcggcggaa
     1081 cgcgtaaaga agtgcgcttc gggcatccgt caggtacgct gcgtgtcggt gctgccgccg
     1141 aatgtcagga cggacaatgg acggccgcca aagcggtcat gagccgcagc gcacgcgtga
     1201 ttatggaagg ttgggtgcgc gttcccgatg attgtttttg aaatcatgta ggacgcacat
     1261 tgctgtccgc tgtttcagac ggcatttgat taaggattac cataaagccg aataacctca
     1321 ccgccgtcat tcccacgcag gcgggaatcc gcagccgata ttctgtttcc tgatttttat
     1381 cagagcctgc gtgtgagcaa atatggattc ccgcctgcgt gggaatgacg ctaggaaata
     1441 cggcatacgc tttgcccaaa gaggccgtct gaaacacact ccgcccaacg cccatccttt
     1501 ttcagacggc cccgcaccaa aaaaacaacc acaaactaca aggagaaaca tcatgtccga
     1561 ccaactcatt cttgtcctga actgcgtcag ttcatcgctc aaaggcgccg ttatcgaccg
     1621 caaaagcggc agcgtcgtcc taagctgcct cggggaacgc ctgactacgc ccgaagccgt
     1681 cattaccttc aacaaagacg gcaacaaacg ccaagttccc ctgagcggcc gcaactgcca
     1741 cgccggcgcg gtgggtatgc tgttgaacga actggaaaaa cacggactgc acgaccgcat
     1801 caaagccatc ggccgccgca tcgcccacgg cggcgaaaaa tatcacgagt ccgtcctcat
     1861 cgaccaagac gtccttgacg aactgaaagc ctgcatcccg ttcgccccgc tgcacaaccc
     1921 cgccaacatt agcggcatcc tcgccgcgca ggaacacttt cccggcctgc ccaacgtcgg
     1981 cgtgatggac acctcgttcc accaaaccat gccggagcgg gcctacactt atgccgtgcc
     2041 gcgcgaattg cgcaaaaaat acgccttccg ccgctacggt ttccacggta ccggtatgcg
     2101 ttacgtcgcc cctgaagccg cacgcatctt gggcaaacct ctggaagaca tccgcatgat
     2161 tattgcccac ttaggcaacg gcgcatctat taccgccgtc aaaaacggca aatccgtcga
     2221 taccggtatg ggtttcacgc cgatcgaagg tttggtaatg ggtacacgtt gcggcgacac
     2281 cgatccgggc gtatacagct atccgacttt ccacgcaggg atggatgttg cccaagttga
     2341 tgaaatgctg aacgaaaaat caggtttccc cggtatttcc gaacttccca acgactgccg
     2401 caccctcgaa atcgccgccg acgaaggccg cgaaggcgcg cgcctcgccc tcgaagtcat
     2461 gacctgccgc ctcgccaaat acatcgcttc gatggctgtg gcctgcggca gtgttgacgc
     2521 actcgtgttc accggcggta tcggcgaaaa ctcgcgtaat atccgtgcca aaaccgtttc
     2581 ctatcttgat ttcttgggtt tgcacatcga caccaaagcc aatatggaaa aacgctacgg
     2641 caattcgggc attatcagcc cgaccgattc ttctccggct gttttggtcg tcccgaccaa
     2701 tgaagaactg atgattgcct gcgacactgc cgaacttgcc ggcatcttgt agccaaaaaa
     2761 gggggcgagt ccgcaaaaat gccgtctgaa aggttttcag acggcatttt gtttggcgtt
     2821 tgggcgtaca atacggaaaa catgacgata aggaaacaaa ctatggcaca atttttcgcc
     2881 gttcatcccg acaatcccca agaacgcctc atcaagcagg cagtcgaaat catcaatcaa
     2941 ggcggcgtgg tggtttatcc gaccgattcc tgttacgcct tgggttgcaa actcggcgat
     3001 aaggcggcga tggaacgcat actctccatc cgaaaaatcg atttgaaaca ccacctgacc
     3061 ctgatgtgcg ccgatttgag cgagttgggc acatacgcca aaatcgacaa cgtacagttt
     3121 cgtcagctta aagccgccac gcccgggcct tataccttta tcttgcaggc gacgaaggat
     3181 gcgccggcgc gcgccctgca cccgaaacgc aaaaccatcg ggctgcgtat tcccgataat
     3241 gccgttgcac aagccctgct ggaggagttg ggcgggccgc ttttaagctg caccctgatg
     3301 ctgcccgaag acggcgaacc attgaccgac ccttatgaaa tccgcgagcg tttggaacac
     3361 gccgtcgatt tggtaattga cggcggctgg tgcggaaccg atccgactac cgtcatcgac
     3421 atgaccgacg gcacggaact ggtgcgccgg ggtttcggcg atacggcggt gttcggtttg
     3481 tagggaaacc gatgccgtct gaagcatcag ccgttcagac ggcatcgcgc gccttgccgg
     3541 cggcagcccg aaatgccggc gcgtatcgcg cccggtcgga atatccgttt gaaacggcaa
     3601 acaaaatgcc gtccgaacgc gggtttgggc ggcattttga tgcgttatcg caccgcaatc
     3661 gggattttcg attcataaag caggtcgtag gtcggcttgt tgagcaggtc ttgcagcgtg
     3721 aaaccgtcca gatacgtgaa aaacgacttc attgcaccgc cgagtatgcc cgtcagccgg
     3781 caggacggcg taatcaggca ttcgttgttc gggcccatac actcgaccag ctgcatcggt
     3841 tcgaggtggc ggacgaccgc gccgatattg atgcgttcgg gcggcgcggc cagcctcagc
     3901 ccgccgcctt tcccgcgtac gctgtgcaag aacccgcctt tgaccagcgc ggtaaccact
     3961 ttcatcaaat ggcttttgga aatgccgtag gtcgaggcga tggtggcgat attgaccagc
     4021 gcgtcgtcgt tgacggcggt gtagatgagg acgcgcagcc cgtagtcggt atgttgggtc
     4081 agatacatga ttttctcggt atggatcgtt attcttatcg gtacggttta agtttcacgg
     4141 aaaatacctt aatggttgaa accctgtccg tcggggcggt agaatgtagc ctgtctgcgg
     4201 cggtatgccg tctgaaacat ccgcgctacc gtttgataat ttgttattgt aacctaaatt
     4261 tatgaaaccg ttgaaacgac attccgccct tgtcgggctt tcgcgcgaac accaccactc
     4321 gctttccctg tgcgtgcgta tgttgcggac gcccggggca gaccatcgtg ccgaactcga
     4381 gctgcatttt gccgaactgg aaacccattt tcgcgaagaa gaagccaagt ttgccccaat
     4441 ttggcagaat gtcgcgcctg agttgaaaac ccgttttgaa ggcgatcacg ccaaactgcg
     4501 gcagatgatg gcaagccccg aatgcggtaa cgcggcgtgg aataccgctt ttgccacaac
     4561 cctgcgcgac cacgcgcgct ttgaagaacg cgagctgttt cccgccgtcg aaccgttttt
     4621 gccggcatga ttccgttttg cggcaaatat attaatgata aacaaggaac acacatgaaa
     4681 tttaccaaac atcccgtctg ggcaatggcg ttccgcccgt tttattcact ggcggcactg
     4741 tacggcgcat tgtccgtatt gctgtggggt ttcggctaca cgggaacgca cgagctgtcc
     4801 ggtttctatt ggcacgcgca tgagatgatt tggggttatg ccggtctcgt cgtcatcgcc
     4861 ttcctgctga ccgccgtcgc cacttggacg ggacagccgc ccacgagggg cggcgttctg
     4921 gtcggcttga ccgccttttg gctggctgcg cggattgccg cctttatccc gggttggggt
     4981 gcggcggcaa gcggcatact cggtacgctg tttttctggt acggcgcggt gtgcatggct
     5041 ttgcccgtta tccgttcgca aaaccggcgc aactatgtcg ccgtattcgc aatatttgtg
     5101 ctgggcggta cgcatgcggc gttccacgtc cagctgcaca acggcaacct aggcggactc
     5161 ttgagcggat tgcagtcggg cctggttatg gtgtcgggct ttatcggcct gattgggatg
     5221 aggattattt cgttttttac gtccaaacgg ttgaacgtgc cgcagattcc cagtccgaaa
     5281 tgggtggcgc aggcttcgct gtggctaccc atgctgaccg ccatactgat ggcgcacggc
     5341 gtgatgcctt ggctgtcggc ggctttcgcg tttgcggcgg gcgtgatttt taccgtacag
     5401 gtgtaccgct ggtggtataa acccgtattg aaagaaccga tgctgtggat tctgtttgcc
     5461 ggctatctgt ttaccggatt ggggctgatt gcggtcggcg cgtcttattt caaacctgcc
     5521 ttcctcaatc tgggcgtaca tctgatcggg gtcggcggta tcggcgtgct gactttgggc
     5581 atgatggcgc gtaccgcgct cggtcatacg ggcaattcga tttatccgcc gcccaaagcc
     5641 gttcccgttg cgttttggct gatgatggcg gcaaccgccg tccgtatggt tgccgtattt
     5701 tcttccggca ctgcctacac gcacagcatc cgcacgtctt cggttttgtt tgcactcgcg
     5761 ctgctggtgt atgcgtggaa atacattccg tggctgatcc gtccgcgttc ggacggcagg
     5821 cccggttgag taaaaccgcc gcagatttcg ggtctgcggc ggtttgcttt tcagacggca
     5881 tggcggtcag ttgccgtcca gccagcggtc gcgcgtggtt ttggcttctt caaaatagcg
     5941 gtacagggct tcgcggtcgt cggcggtcag gatgtctgcc aaaacgtcca actgtttgcc
     6001 caagccttga accagttgca gcaggctgtc tttgttggca aggcagatgt ccgcccacac
     6061 ggcgggatgg ccggaggcga tgcgggtgaa gtcccgaaag cccgtggcgg cgaatttcag
     6121 atattcctgt ccgtcggggt ggtcgagaat ctggtggaca taggcgaagg cggtcaggtg
     6181 gggcatatgg gagacggcgg cgaaaaccgc gtcgtggtgt tgcgcgtcca tcgtgaaaat
     6241 gtccgcaccg accgcgcgcc acaggttttc taccaatgcg atgccgtctg aatcttcgcc
     6301 gccgtggggc gtgatgatga gttttctgtg gcggaacagc ccgaactgcg cggcttgcgc
     6361 accgcttctg tccgaaccgg caatcgggtg tgcggcgatg cagcggtgca ggcggtcggg
     6421 caggcagcgg cggaaggctt cgatgaccga agatttggtg ctgccgacat cggaaatcca
     6481 agtgtgttcc ggcaaaacgg ggcgcagcgc ggttaaaacg gcgggaacgg tggcgacggg
     6541 cgtggcaatc agtaccaagt ccgcaccgcc gatgctgtcc gcgtcgatga cgacggaagc
     6601 ccggttaatc acgccgcgtt ccaatgcacg ttcgaggttg tcgcggtcgg tgtcgatacc
     6661 ggtaacggtg cggacgagtc cctgcctttt gaggtcgaga acgaacgaac cgccgatcag
     6721 ccctacaccg atgagggcaa tatggttcaa aatgggcatt tgtgtaaacg gttttcgcaa
     6781 agtgccgcca tggtagccta tcggcggaat atgccgcaag gtcggcagga aaaaggagaa
     6841 gaaatggaca aaatcagagt tgccgccgtg cagatggtgt cgggcgtgtc gccggaaacc
     6901 aacgtcgccg ccatgaaacg cctggtcgca cgggcggcgg agcagggtgc ggattgggtg
     6961 ctgctgcccg aatattgggt gctgatgggc gcaaacgata ccggcaaact cgcacttgcc
     7021 gagcctttgg gcggcggacg ctttcagacg gcattgagcg aaacggcgaa agaatgcggc
     7081 gtggtgctgt tcggcggaac cgtgccgctg caaagccccg aagcgggcaa ggtcatgaat
     7141 actttattgg tgtacgggtg cgacggcgta aggacggggc tgtaccacaa aatgcacctc
     7201 ttcggttttt ccggtttggg cgaacgctat gccgaagccg ataccatccg cgcagggcgg
     7261 gaagtgccgc acttgtcggc agaaggcatg ccggtggcgg caggtatttg ttacgatgtc
     7321 cgctttcccg aatttttccg ccgccaactg ccgtttgacg tattgatgct gccggcggca
     7381 tttacccata cgacgggcaa ggcgcactgg gagctgctgc tgcgcgcgcg tgccgtcgaa
     7441 aaccaatgtt acgtcgtggc ggcggcacag ggcggtttgc acgaaaacgg acggcgcacg
     7501 ttcggacaca gtatgattgt cgatccgtgg ggcgacgtgt tggacgtatt acccgaaggc
     7561 gaaggcatcg ttacggcaga catcgatgcc aaccgcctga acagcgtccg caaccgcctg
     7621 cccgccttga aacaccgggt tttg
//
LOCUS       DAXDZG010000083         7615 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXDZG010000083 DAXDZG010000000
VERSION     DAXDZG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.083"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..1716)
                     /locus_tag="ACFB78_001801"
     CDS             complement(<1..1716)
                     /locus_tag="ACFB78_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC1269742.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGENLADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLNHFVV
                     LESVSSDGAAVMDPASGRRKVKTEEISRKFTGIALELWPNTRFEAGEEKQEIRILPML
                     RGISGLGRTLFQLLALAAAMEVFALVSPFFMQWVIDHVVVTGDRNLLATLALGFTLLL
                     LVQNTVSVMQGWLGMHFSTTLNVQWKSNVFKRLIDLPTDYFAKRHLGDVVSRFGAVDS
                     IQGTLTSTFFVLVLNSLTAVFTFVLMIVYSPILTGLVAATLVVYIAVRWVAYYPLRRA
                     TEENIIHAAKQSSYFMETVRGIRAVKQFGKGPQRYSAWMGLLVDTVNTGLTVQKLGIW
                     FGLANKLLFGLANILIIYLAAGMVLDGVFTVGVLMAFLAYKGQFEGRIGSLVDQFVQI
                     KMLSLHAERLADTVLNETESEATPDVGIPDISDDIEISVENVSFRYADNEPYVLQNVS
                     FKIGRGESLALIGRSGCGKSTFLDILSGNLPPESGKVMINGHDIYSLPPRFIRNLSAM
                     VRQDDV"
     gene            complement(1818..2033)
                     /locus_tag="ACFB78_001802"
     CDS             complement(1818..2033)
                     /locus_tag="ACFB78_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269743.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(2047..2394)
                     /locus_tag="ACFB78_001803"
     CDS             complement(2047..2394)
                     /locus_tag="ACFB78_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269744.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(2579..3853)
                     /locus_tag="ACFB78_001804"
     CDS             complement(2579..3853)
                     /locus_tag="ACFB78_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC1269745.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(3914..4186)
                     /locus_tag="ACFB78_001805"
     CDS             complement(3914..4186)
                     /locus_tag="ACFB78_001805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269746.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(4261..4488)
                     /locus_tag="ACFB78_001806"
     CDS             complement(4261..4488)
                     /locus_tag="ACFB78_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269747.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(4497..5651)
                     /locus_tag="ACFB78_001807"
     CDS             complement(4497..5651)
                     /locus_tag="ACFB78_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC1269748.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            5903..6184
                     /locus_tag="ACFB78_001808"
     CDS             5903..6184
                     /locus_tag="ACFB78_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269749.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            6181..7224
                     /gene="argC"
                     /locus_tag="ACFB78_001809"
     CDS             6181..7224
                     /gene="argC"
                     /locus_tag="ACFB78_001809"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC1269750.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         1950 a         2014 c         1688 g         1963 t
ORIGIN      
        1 aacatcgtcc tgcctgacca tcgcactcaa attgcggata aaacgtggcg gtaagctgta
       61 aatgtcgtgc ccatttatca tgactttgcc tgattcggga ggtagattgc cgcttaaaat
      121 atccaaaaat gtcgatttac cgcagcccga tcgtccgatt aacgcaagcg attcaccacg
      181 tccgatcttg aagctgacgt tttgtaaaac atatggctcg ttatcggcat aacggaaaga
      241 aacattttcc actgagattt cgatgtcgtc tgaaatatca ggaatgccta catccggcgt
      301 agcttccgat tctgtttcat tcaatacggt gtcagctaac cgttccgcat gaagtgacag
      361 cattttaatt tgcacaaact gatcgaccag cgagccaata cgtccttcaa actgtccctt
      421 ataagccaaa aaagccatta gtacgccgac ggtaaagacg ccgtccaata ccatgcccgc
      481 agctaagtaa ataatcaaaa tatttgccaa accgaaaagc agcttgtttg ccaagccgaa
      541 ccaaattccc agtttctgca ccgtcagtcc cgtattaacc gtatccacca gcagccccat
      601 ccatgcagaa tatcgttgag gacctttacc aaattgcttg actgctcgga taccgcgtac
      661 cgtttccata aaatagctgc tttgtttggc agcatggatg atgttttcct ccgtcgcccg
      721 tcgcaacgga taataggcta cccaacggac tgcgatataa actaccagtg tcgctgcaac
      781 caaacctgtc agtatcgggc tgtacactat cattagcaca aaagtaaaaa cagccgttag
      841 gctgtttaaa accaacacga aaaacgttga agtcagagtt ccctgaatac tatccaccgc
      901 cccgaagcgc gataccacat cgcccaaatg ccgtttagcg aaataatctg taggcaaatc
      961 tatcaaacgt ttgaaaacgt ttgatttcca ctgaacattt aacgtcgttg aaaaatgcat
     1021 gcccaaccag ccctgcatga cagacaccgt attttgtacc agaagtagca aggtaaagcc
     1081 caaagccagc gtcgctaaca gattacgatc gcccgttacc actacatggt cgatgaccca
     1141 ttgcataaaa aacggcgaca ccaaagcaaa cacttccatt gctgctgcca aagccaaaag
     1201 ctgaaacaat gtccgcccca gcccagaaat cccgcgcaac atgggtagga tgcggatttc
     1261 ctgcttttct tcccctgcct cgaaacgcgt gtttggccac agttccaaag caattcccgt
     1321 aaacttgcgc gatatttcct ccgtcttgac tttgcgtcgt cccgaagccg gatccatgac
     1381 ggcagccccg tccgaagata ccgattccag caccacaaaa tgattcaaat cccaatgtag
     1441 aatacagggc aggcgcaaac tgcccaattc gtctaaatcc agcctcaacg cccgtcccgt
     1501 cagccccata tcatcagcaa aacgaacaat atctgccaaa ttctcaccct taagtgacag
     1561 acagtatttt gaacgcagtg cgcgcaaatc cgtatagaaa ccataaaatc cggccacagc
     1621 cgctagacat gccaagccac actccgctac ttctgtttgc agtataacgg gcagcttttt
     1681 tgtcaagccc aaagacaggt tttgcagata atccatagat ttccttgttg aatcagaaaa
     1741 gagagatggg aagacgggaa tggtttggaa aggccgtctg aaaaggtttc agacggcctg
     1801 cagccccata ttgactttca atgcgaccgg ttttcctcgc gtttcaaata cagcagatac
     1861 gcaagaaaag acaaagcccc tacaggtcct gcgacggtaa ccgaaaaaaa cctgcctttg
     1921 taaaacacac cgtcgaagtt taaaaaatag caaaggatgt cggtcaggat gctgatggca
     1981 agataaatca gcacagcggc tttccagtgt ttcaggtaat gtgcgaaatc catttcaaac
     2041 tcccgatcaa cagttgcacg ggtcgcaatg gcagtcttct ccgcattggc agttgcgcct
     2101 gtcgatcatt tttccccatt ttgcaccggc aaaaccgccg atatgcccac ccaatgcaga
     2161 accgccctca gctcctaccc atccgccgcc tgcgccgccg atggcaattc cggcaggatt
     2221 tccgcctgtt accaatgcgc cgcccgccat tcccgtgaat gctccggcaa acgaccctaa
     2281 agcgccgccg accttgcttc cgacccattc tccggagact ctgcccctgt gcccccaagt
     2341 accttctgtg ccgccgaaaa cagcggtcat ttcgttcatg gtcaattctc tcatttcaaa
     2401 actcctttta tcgtcaatcg aaaaattgaa agggcagata ccctatcctt gcttttacag
     2461 acagagtatc tgtaaaaggg ggtagtttca ggcagcttca gtgtttatgt ttttatgtag
     2521 tgttttacag gtcgtctgaa gaggtttcag acgacttttt atctttcagc ctcaagcctt
     2581 acaacctgcc cgacatacta taaatcggct ccaataccca ttcgtacagc cgccgtttct
     2641 cgtgtaggat gtctgcttcc aacgtcatgc cgatttgcag cggtttttcc tcaccgtatg
     2701 cagtgatggt tggtttgtcg ggttttattt tcacgagata aacaggttcg ttgctcttcg
     2761 ccaaatcgga ggataccatg cccaatcccg acaattcctg tctgcccagt gccgtttttg
     2821 ccactgatac gacactgccg gaagcaagcc cgaatttttg atagggatat gcctgataac
     2881 gcaggacaac cttgtctttc ggcttgataa agcctgctgc actgctgggg atatatagat
     2941 gggcatataa ctcggtacgt tcgggaacaa tgctcaagaa cagtttggaa ggatcaacct
     3001 gccgtccgac ttcgacgttc ggtattgcta tataacccga ccgtcctgca cggatgattt
     3061 gttcagagcg catttcaaaa tccaaaactt cttgagaaat atcggcaatg gtgcgttcaa
     3121 gctggctttg ttctgtctca tgccgtttgg ggaggctggc caatgtcaga ttctgcgtgc
     3181 ggatttcctg aagcagcccg gcttcttctc ggcggtaggc atcaagtttg gctttctgct
     3241 ctaaaagctc tgcctcgaca ttcatcattt cttgttttga cactgcatca ttggcggata
     3301 ggaaacgata tttccgcaac atttcttccg caagtctaat gcgccttttc tggccgtcta
     3361 tctgttgcga aatatggagt ttctggtttt ccaaacgttc gacagttgct ttaaggctgc
     3421 gcgtttcatt ctcgtgtatc agcttcagac gacccagttc ctgttctgcc aacgttttct
     3481 tcaaaactgc ctccgttttc aactgctgct gcacgctacc tcctgcgccg aaacgtgagg
     3541 tcgaaagcgc aaatagcttg tcgccagcct taaccttttc tccatcttcc acgaatttcg
     3601 ctgtaattgt ccccgtatcc ggtgcataca ccctgattac gcccgatgca ggtagaattt
     3661 gtccctccat tgttgtcttt cgcgtatagt taccgaatat caaaaacagg ataatcagta
     3721 acgcagatat cgatgcaaat gtcgtccaca gggaaaatga caacgggcgt gtcagaatca
     3781 ctttacccgt caggctggtt tggcgggcaa tggcgacttc agggcggaag aaaggttgct
     3841 tgggtctatt cataaaattg aagttaagaa agtttcagac gacccctaga gatcgtctga
     3901 acaatgagaa atatcagcag taatctgtac cgtcagtgta gccgtttcct gatttatctg
     3961 cttttgttgc gggagcagtt aatccatgtt caatctcaaa gattggtctt ccgttataag
     4021 gaggtgcatt aacggcatca tttacccaat cacgagtcag attgtataca ccatttgcac
     4081 catatagcac cgaaaaaatt tttcggcagg aaatgaactg tcgctgcagc agcaggtatt
     4141 gcaacaaaat ccccccatgt gggacctcct ttggttgtgg cagcattagc tacatttcca
     4201 gctatattgt ctgttacagg acctccccct gaaaccagct tcaattcatg aagttgaagt
     4261 tcaatcattt ttatactcct tttcttggtt ggtattccta aaaattcggc taacaaaaac
     4321 atatggcaga tatattgaaa aaaaattcaa agtaccctga ataaaattca aattccaact
     4381 atatttgtta atgtagtcga gaagaaacat atctgataaa gaatatagca attgataaca
     4441 agctattact aatattacga aaaatataaa ctgcttccag tttttcatag aatccctcac
     4501 aaaatttcca gaaaatctaa ctctatcaac tgatagatca acttcctaac ttcttcatgt
     4561 tttgcctgat tgaagttaac cagtagattt ttcaacaata acggttcatt cttaccgatg
     4621 tgttctaaca cttttttccc caactcatct acgcttatct tcatcccatt cccaatcaaa
     4681 tatccctttt ccaacgtatc caaattattg gcatttagtc tcaacctgac gtcgtctgaa
     4741 agcggagtag cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc ggtacgttct
     4801 ttgccgagaa agtcttcact gaaagcttcg taattgacgg ggtcggcaat catggcggca
     4861 atttgtgcgg cagtatcgtt gatacgcgtc ctatcttgct cccagtctga gaaactgtgg
     4921 cgcagacttt ctatggtggg aaatttcttc attagccact cgaggtaatt atagccgttt
     4981 ggtgggaagg taccgacagc gaagtggaag gtttcacagc cgagcgggat aggtctgtgc
     5041 caccaaccgc gtgggatgta gaggacatca cctgcttcaa ggataatatc catatcgata
     5101 tgttcaggaa tggaaatatc agtatcttta gtctgttgca tatacaatgg catagggaaa
     5161 tcaggggcag taagttgcca acgtttcttg ccgaaaagct ggatggcata cacatcgcgg
     5221 gtatcccaat ggtttttata agattcgtcg ctgccaaaag caagatatcc actaacaata
     5281 gtatgtgcgc cggcaaagcg ggcgacttga cgggcgatat ggtctgaaaa cggctcgttg
     5341 ttaatatggt tatagactaa cgacgcacca ttcttcatat gttcgtagat aacggattta
     5401 ataaaacggt agcgagtttt gcccaaatcg tcgaaacttt cgacgtattc ttctttagga
     5461 acgattgcgc cttttttacg cagatgaaac agcggtgcgg ttgggtctgc tcgttggtat
     5521 atctcgttga tatctttcca agatgcggct tcgagattcc gaaccgctcc tttaaagagc
     5581 ttgggctttt gatacagata agtctgtcgg aacgttttag gactaatgcc gaagtcgaga
     5641 tggatgccca ttacttcccc ttactcagaa aatatttaaa atttataatg ttacatatag
     5701 ttacaaatat tagagttttt tgtgtgtgcg tcaaggaatt gttgacaatt ttagttaaaa
     5761 atttgtctcc aaacggaaaa aagcggtttg ttttttgttg ttaacatttt tattgtaatt
     5821 aaataaaagg tcgtctgaaa acggttttca gacgaccttt tgctataatc gggcttcatc
     5881 gccccgttcg gtttggaacc ttatgaaaac cctcgtcctc ctcctgcttt ccttctccac
     5941 gaccaccgct ttcgccgcat acggtttggg tttggggcag gcaccgaaat atcctgccgg
     6001 ctttcgcgcc tacggttatg tttattccgg acggcagggc tgggttttaa aaacagaggc
     6061 ggatgccatt aaattagaca cgcttttcaa acgctttgtg taccgtcctt ccgccgccaa
     6121 tcaaaacccc gtcggacagc gttcggacgg catacccgcc aaccacacaa aggaaaaacc
     6181 atgagcaaaa aaatcaaagc cggcattgtc ggcgcgacgg gctacacggg cgtggaactg
     6241 ctgcgcctgc ttgccgccca tccagatgtc gaagtcgccg ccgtaaccag ccgcagcgaa
     6301 gcgggaaccg cagttgccga ttactttcca agtctgcgcg gcgtgtacgg cctcgccttc
     6361 caaacgcccg acgaggcagg tttggaacaa tgcgacatcg tcttcttcgc cacgcccaac
     6421 ggcatcgcca tgaaagacgc gccgcgcctg attgaacagg gcgtgcgcgt catcgacctt
     6481 tccgccgact tccgcatacg ggacattccg acctgggaac actggtacgg catgacccac
     6541 gccgcccccg gcctcgtttc ccaagccgtg tacggattga gcgaactcaa ccgcgaagcc
     6601 gtcgcacagg cgcgcctcgt cgccaacccc ggctgctacc cgacctgcgt atccctgccg
     6661 ctcgtgccgc tgttgcggca atgccgtctg aagcccggta tgccgctgat tgccgactgc
     6721 aaatccggcg tgtccggcgc gggcaggaaa ggcaatgtcg gttcgctgtt gtgcgaagcc
     6781 ggcgacaact tcaaagccta cggcacagcc ggacaccgcc acctgcccga aatcaggcag
     6841 accatcgccg ggcttcagga cggcatcgcc gaaggattcg tgttcacgcc gcacctcgcg
     6901 ccgatgatac gcggtatgca cgccaccgtt tacctccacc tttcagacgg cagcgacccc
     6961 gaaaccgtcc tgcgcgacta ctaccgcgac agcccgttca tggacatcct gcccgccggt
     7021 tcgacacccg aaacccgcag cgtgcgcggc gcaaacctct gccgcatcag catccgtcag
     7081 gcggcgcaat ccgatgtgtg ggtcgtcctt tccgtcatcg acaacctcgt caaaggcgcg
     7141 gcgggacagg cggtgcaaaa tatgaacatt atgttcggac tggaggaaac acacggcttg
     7201 ggcggcatcc ccctgctccc ctgaagcgca aacggcaaac cgcaggcatc gtgcctgcgg
     7261 ttttttgatg ccgtctgaaa gcggcggttt ttttgggttc ggacggcttt tggctcatca
     7321 ttccgcgaaa acaaaaatct aaaataccgt cattcccgca aaagcgggaa tctagtttat
     7381 ccggcttcag caatttccga cacatttcca cacgcttcga ttccgtcatt tctccggttt
     7441 caatcgtttc cgataacacc gtggtttttc atttctagat tcccgcctgc gcgggaatga
     7501 cggcgggggg cttgccgttt tcccgataag cctctgcggc ttctcattgc cggatttccg
     7561 cccgcttggg aataacggga tttaaaatca tgaaaatgtg tcaaaaataa tatag
//
LOCUS       DAXDZG010000084         7597 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXDZG010000084 DAXDZG010000000
VERSION     DAXDZG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.084"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            563..967
                     /locus_tag="ACFB78_001810"
     CDS             563..967
                     /locus_tag="ACFB78_001810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC1269751.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            1007..2188
                     /locus_tag="ACFB78_001811"
     CDS             1007..2188
                     /locus_tag="ACFB78_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC1269752.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(2291..4546)
                     /locus_tag="ACFB78_001812"
     CDS             complement(2291..4546)
                     /locus_tag="ACFB78_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC1269753.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4920..6098
                     /gene="nirK"
                     /locus_tag="ACFB78_001813"
     CDS             4920..6098
                     /gene="nirK"
                     /locus_tag="ACFB78_001813"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC1269754.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAKNPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            6276..7031
                     /locus_tag="ACFB78_001814"
     CDS             6276..7031
                     /locus_tag="ACFB78_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC1269755.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNISWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKDRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            7280..7597
                     /locus_tag="ACFB78_001815"
                     /pseudo
     CDS             7280..7597
                     /locus_tag="ACFB78_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         1924 a         2133 c         1903 g         1637 t
ORIGIN      
        1 agacggcatt gttctcccgc cgtcattccc gcgcaggcgg gaatccggaa cgtaaaatct
       61 aaagaaaccg ttttatccga taagtttccg tgccgacagg cccggattcc cgcctgcgcg
      121 ggaatgacgg gattttaggt ttctgatttt ggttttctgt ttttgaggaa atgacgggat
      181 tttagtaacc gtaacaactg cctgcgcgac ggctaagggg cttcagcaac cgtagcaact
      241 gcctgtgtgg gaatgacgga caatgggctt cagacggcat ctcttgcctt ccgctaaaac
      301 agtttgccgc acaactgttc aaacgcgtcc gatatgtttc aacacacagg acgacacata
      361 aagcaccgcc ctatatgtcg tcctgattcg gaaggggtta cgcccctccc aaacaaagtc
      421 tgatcctgcc gccctaaagg gcggggtttc aaccgaaaag gaaacacgat gaagtggtac
      481 aattaacggc aatgcggaca gacaaattaa actatcagaa attgccctac cgttttttaa
      541 acactttcag gaataaggaa aaatgaccgc ccaaccctgc cccatctgca cggcacaaga
      601 tgaagatatt ttgctgcaaa cccccaagct gcgcgtcatc gccgtccata acgacagcgg
      661 ttcgccggca ttctgccgcg tcatttggca cggacacatt gccgaaatta ccgacctttc
      721 ggcagcggaa cgcggcgaat tgatggaaat ggtgtacaaa gtcgaagccg ctatgcgcca
      781 agtgttccgg ccggcaaaaa tcaacctcgc cagcttgggc aatgtcgtgc cgcacctgca
      841 ttggcatatt atcgcccgct ttgaaaacga tgcgactttc cctgcgccga tttgggcaaa
      901 ccccgtccga aaacacggta tgaccctgcc gcaaaattgg acggaacagc ttaaaaagct
      961 gctttaaacc cgccgatgcc gtctgaaacc gtatgaaagg gaaattatga ccgaaccgac
     1021 ctcccgccgc cgttttctga aaacctgcac cgccgccggc gcggggctgc ttcaggcttg
     1081 cggcacatcc gccacatccg ttccgcccct tccctcttcc cattccgttg tgaaagcccg
     1141 aaccgtgcct ctccaaacgc cacgccgtca aagttcggac ggcaaccttc tgcgcgttgt
     1201 cgcttcgtca ggatttgccg aagacaccaa ccgcgtcaac acagccttaa cccgccttta
     1261 caatgccggt tttaccgtaa ccaaccaaca ggcgggcagc cgccgtttcc aacggtttgc
     1321 cggcacggac gcgcaacgcg cggcagattt ccaagaggtc gcctccggcc gcgtcgccac
     1381 gcctaaagtg ctgatgggct tgcgcggcgg ttacggtgcg gcgcggattc tgccgcatat
     1441 cgattttgcc tcgctcggcg caaggatgcg cgaacacggc acgctgtttt tcggattcag
     1501 cgacgtatgc gccgtccaac tggcattgtt ggcaaaaggc aatatgatga gttttgccgg
     1561 cccgatggct tatagcgatt ttggcaaacc tgcccccggt gcgtttacga tggatgcctt
     1621 tatcaagggg acaacccaaa accgcctgac cgttgatgtt ccttatatcc aacgcgccga
     1681 tgtcgaaacc gaaggcacgt tgtggggcgg caacttaagc gtcctcgcct cgctcgccgg
     1741 tacgccttat atgcccgaca tcgacggcgg cattttgttc ctcgaagatg tcggcgaaca
     1801 gccctaccgc atcgaacgta tgctcaatac gctgtatctt tcgggtattt tgggcaaaca
     1861 gcgcgccatc gtgttcggcg atttccgtat ggaaaaaatt cgagatctct atgattcgtc
     1921 ttatgatttt tccgccgttg ccaagcatat ttcgcgcacg gcgaaaatcc ccgtgctgac
     1981 gggcttcccg ttcggacaca ttgccgacaa aatcactttc cctctgggcg cgcacacccg
     2041 aatccgtatg aacggaaacg gcggctattc ggtcgcgttt gaaggctacc ccacactcga
     2101 tgcgtccgcc ctgactttgg ataccctgct cccaccgccg gatttgccta tcttccccga
     2161 aagcggtgtt gccgatattt cggaataaac ccgcaaacgg acaaatgccg tctgaagcct
     2221 tcagacggca ttttcctgta aaaaaaaccg cacctgatgg caggtgcggt ttccattttc
     2281 aggctaaaac ttatttgtcg cggccgaata cgattttagt ggcttggatg gcaacgcaga
     2341 ttgcaccgcc gataaagatt aagtcggggg cagtgcgtac ccagcgcagg gtgtcgagga
     2401 tttccatttg caggaattct tcgctgcggg cataccacaa accgtgcgtg atagaggcgt
     2461 atgcctgaat cacgccgaca ggcagcaggc tgatggcaat cataccgacc aagccgccgt
     2521 tgagcagcca gaagccccaa gtcatcagtt tgtcgtcaaa ccgcgcgttc ggtttcaagt
     2581 agcgcgcaac caacaatacg aagcccaatg ccaagaaacc gtacacaccg aacaaggcgg
     2641 cgtgcgcgtg aacggcggaa gtgttcaaac cttggatata gaacagggaa atcggcggat
     2701 tgatcagaaa gccgaatacg ccggcaccga tcatattcca aaaagcgact gccacgaagc
     2761 acatcagcgg ccaacgcagg cgtttcgccc agtcggacag gtgttggtaa gaccagtgct
     2821 cgtatgcctc gcggcccagc aacaccagcg gcacgacttc caaagcggag aagcaggcgc
     2881 cgattgccat agaggcggag gtagagccgg agaagtacag gtggtgcagc gtgcccggaa
     2941 cgccgcccaa cataaagatg gcggcagcgg ccaaagtgga ggcagtggcg gtactgcggc
     3001 ggacaaagcc catattgtag aagacaaagg caaaggcggc agtggcaaat acttcaaaga
     3061 agccttccac ccacaggtgg accacccacc aacgccagta ttccattacg gcaatcgggg
     3121 atttttcgcc atagaacagg cccggtgcgt agaacacgcc cacgccgacc atagaagcga
     3181 caaagattgc cagcaagttt ttgtccacgc ctttttcttt gaaggcggaa accgtgcagc
     3241 gcaacatcag gaacagccac aacagcagtc cgaccatcaa aaggagttgc cagaaacgtc
     3301 ccaaatcgag gtattcgtaa ccttggtgtc cgaaccagaa gttaaattcg ggaggaagga
     3361 tgtgcgtcaa cgcgaagaag ttgcccgcgt aagaaccgcc gaccacgatg aagagggcga
     3421 tatagaggaa gttcacgcct gcacgttgga acttgggatc tttgccgccg ttgacaatcg
     3481 gcgcgaggaa caaacctgcc gtcaaaaagc cggttgcaat ccagaagatg gcggattgga
     3541 tgtgccaagt acgggtcagg gcgtagggga accagtcgga catttcaaag cccaacgcct
     3601 cgtcaatgcc gtagaaaccc tgaccttcga cggtgtagtg cgcggtcagg ccgcccagca
     3661 atacttgtac cacaaacagg gcgaccgtca ggaagacgta tttgcccaat gctttttgcg
     3721 aaggggtcag ttggattttg gaaatcgggt cttcagacgg cacttccact tcctcgtgtt
     3781 tggtcaggaa ggaataaccc cacatcaaca aaccgatgcc catcaagagc agtacgacac
     3841 tggtaaacga ccacatgtag ttttcagtag tcggtacatt gttgatcaaa ggctcgtgcg
     3901 gccagttgtt ggtgtaggta aagacctcgc cgggacggtt ggtcgaagca gaccaagaag
     3961 tccagaagaa gaagtcgaac agtttttcac gcgcttcttg gcttggcaat gtgttgtttt
     4021 tcattgcaaa gtgttcgcgg gtggtttgca acttgggatc atcgccgtaa acgccgtgat
     4081 agtaaggcag gatgctttcg atggctttca cgcgcgtatc gctgatgacg acgctgccgt
     4141 cttctttaat acggctttga ttgcggtatt catcggccaa gcgggttttc agaacggctt
     4201 gttcttcagg ggaaacttca tcgaattttt tgccgtaagc ctgttgcgcg gtcaaatcca
     4261 accaggcgga caactcacga tgcagccagt cggccgtcca atccggagcc tgatatgcgc
     4321 cgtgtcccag aatcgaaccg acttccatgc cgccggtagt ctgccacgca gactgacctg
     4381 ccaaaatatc gtctttcgtc atcagcactt tgccggatgc ggaaacgacc tgttcggggt
     4441 aaggcggggc tttcttataa acctcgctgc ccatatagcc aagaatggta aagcataccg
     4501 ccagaacggc aaacagcaag taccacagct tcttgtactg tcccattttg agagctcctt
     4561 ttaataaatc agttgtttaa aattcacaaa atatgaatgt taaagattgt agcatggttt
     4621 accgcgcaaa taaacatttg ttcaaacaaa ctcacatata gaacaaatac atatatgata
     4681 ataactatca ttattcttta ctcggaaatt gccctgcctt tgcctgctct gccggagccc
     4741 ctagcaaatc agcctattca ttgtaatttt tagtagttat aaagtattag aagcgtcatt
     4801 ttaagttcat attttatgaa ttatttgact taaattaaaa tgcccgcaat gggacaaccg
     4861 cataatcaca ccaagttctt aactaatccc ccctactttt cttacaaaag gaaaacatta
     4921 tgaaacgcca agcattagcc gcaatgattg cttccttatt cgcattggcc gcctgcggtg
     4981 gcgaacaagc tgctcaagcc cctgccgaaa cccctgccgc ttccgcagaa gccgcaagtt
     5041 ccgccgcaca agctaccgcc gaaacgcctg caggcgaact gcccgtcatc gatgcggtga
     5101 ccacccacgc tcccgaagta cctcccgcaa tcgaccgcga ctatcctgcc aaagtacgcg
     5161 taaaaatgga aaccgtcgaa aaaaccatga aaatggacga cggggtggaa taccgctact
     5221 ggacatttga cggcgacgtt ccgggccgta tgatccgcgt acgcgaaggc gatacggttg
     5281 aagtcgaatt ctccaacaat ccttcttcta ccgttccgca caacgtcgac ttccacgccg
     5341 caaccggtca gggcggcggt gcagccgcga cctttaccgc cccgggccgc acttccacat
     5401 tcagcttcaa agccctgcaa ccgggcctgt acatctacca ctgcgccgtc gcgccggtcg
     5461 gtatgcacat cgccaacggt atgtacggtc tgattttggt cgagcctaaa gaaggcctgc
     5521 cgaaagtgga taaagagttc tacatcgtcc aaggcgactt ctacaccaaa ggcaaaaaag
     5581 gcgcgcaagg cctgcaaccg ttcgatatgg acaaagccgt tgccgaacag cctgaatacg
     5641 tcgtattcaa cggccacgta ggcgctatcg ccggcgataa cgccctgaaa gccaaagcag
     5701 gcgaaaccgt gcgtatgtac gtcggtaacg gcggcccgaa cttggtgtct tccttccacg
     5761 tcatcggcga aatcttcgac aaagtttatg ttgaaggcgg caaactgatt aacgaaaacg
     5821 tacaaagcac catcgtgcct gccggcggtt ctgccatcgt cgaattcaaa gtcgacatcc
     5881 cgggcagcta cactttggtc gaccactcca tcttccgcgc attcaacaaa ggcgcgttgg
     5941 ggcaattgaa agtagagggt gcgaaaaacc ctgaaatcat gactcaaaaa ttgagtgata
     6001 ccgcttacgc cggcagcggc gcggcttctg cccctgctgc ttccgcaccg gctgcttctg
     6061 ccccggcagc ctctgcatcc gaaaaaagcg tttattaaat cggatacccg tcattagcgg
     6121 gacaaaccac tgccgccgta cttcattacg cacggcagtg gttttttaac aaccaatctt
     6181 tcctttcgga agattgattt ttcagacggc attcgcggct ttttgtttaa aatgccgtct
     6241 gaaaaatcaa tgctaaccgc ctgtcaggag gctttatgaa gtacgtccgg ttatttttcc
     6301 tcggcacggc actcgccggc actcaagcgg cggctgccga aatggttcaa atcgaaggcg
     6361 gcagctaccg cccgctttat ctgaaaaaag ataccggcct gattaaagtc aaaccgttca
     6421 aactggataa atatcccgtt accaatgccg agtttgccga atttgtcaac agccaccccc
     6481 aatggcaaaa aggcaggatc ggttccaaac aggcagaacc cgcttacctg aagcattgga
     6541 tgaaaaacgg cagccgcagc tatgcgccga aggcgggcga attgaaacag ccggttacca
     6601 atatttcctg gtttgccgcc aacgcctatt gcgccgcaca aggcaaacgc ctgccgacca
     6661 tcgacgaatg ggaatttgcc ggacttgctt ccgccacgca gaaaaacggc tcaaacgaac
     6721 ccggctacaa ccgcactatt ctcgattggt atgccgacgg cggacggaaa ggcctgcacg
     6781 atgtcggcaa agaccgcccg aactactggg gtgtttatga tatgcacggg ctgatttggg
     6841 aatggacgga agatttcaac agcagcctgc tttcttccgg caatgccaac gcgcaaatgt
     6901 tttgcagcgg cgcatctgtc ggggcgagcg actcgtccaa ctatgccgcc ttcctccgct
     6961 acggcatccg caccagcctg caatccaaat atgtcctgca caacttgggc ttccgctgcg
     7021 caagccgata accccttcaa ttatagtgga ttaacaaaaa ccagtacggc gttgcctcgc
     7081 cttgccgtac tggtttttgt taatccgcta tattccgcca tctctaagat ttacagcgat
     7141 acacgggtaa tttaaggaat gcccgaaccg tcattcccgc aacttttcgt cattcccgcg
     7201 aaagcgggaa tctagaatct cggactttca gataatcttt gaatattgct gttgttctaa
     7261 ggtccggatt cccgcctgcg cgcaagtttc cgaagccatc cttttggccg aaggtcaaaa
     7321 atcagccgtt accgagtatt acctgaataa cggcgaatgg ccggaaaaca acacttctgc
     7381 cggcgtggca tcctccgcca ccgacatcaa aggcaaatat gttaaggaag ttaaagtcga
     7441 aaacggcgtc gttaccgcca caatggcttc aagcaacgta aacaaagaaa tcaaagacaa
     7501 aagactctcc ctgtgggcca agcgtgaaaa cggttcggta aaatggttct gcggacagcc
     7561 ggttacgcgc gccgccaaag acgacgacac cgttgcc
//
LOCUS       DAXDZG010000085         7535 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXDZG010000085 DAXDZG010000000
VERSION     DAXDZG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.085"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(81..497)
                     /gene="dksA"
                     /locus_tag="ACFB78_001816"
     CDS             complement(81..497)
                     /gene="dksA"
                     /locus_tag="ACFB78_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC1269756.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(645..1454)
                     /gene="proC"
                     /locus_tag="ACFB78_001817"
     CDS             complement(645..1454)
                     /gene="proC"
                     /locus_tag="ACFB78_001817"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC1269757.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLEKELGVETSATLPELHSGDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1485..1892)
                     /locus_tag="ACFB78_001818"
     CDS             complement(1485..1892)
                     /locus_tag="ACFB78_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC1269758.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1892..2578)
                     /locus_tag="ACFB78_001819"
     CDS             complement(1892..2578)
                     /locus_tag="ACFB78_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC1269759.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2698..3741
                     /gene="pilT"
                     /locus_tag="ACFB78_001820"
     CDS             2698..3741
                     /gene="pilT"
                     /locus_tag="ACFB78_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC1269760.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3905..5131
                     /locus_tag="ACFB78_001821"
     CDS             3905..5131
                     /locus_tag="ACFB78_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC1269761.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5248..7398
                     /gene="yccS"
                     /locus_tag="ACFB78_001822"
     CDS             5248..7398
                     /gene="yccS"
                     /locus_tag="ACFB78_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC1269762.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIRRLLEMQ
                     GQACRNTAQAIRSGKDYTYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1815 a         2541 c         1667 g         1512 t
ORIGIN      
        1 ctcttctcct ccggctgatg cgcctttgtg aatatgccgc ctgaaactcg gggcttcaga
       61 cggcattttg tttgcctcca tcagtcggca aactgtttct tcatcctctc tcggcgttct
      121 tgggcctcaa cggataaagt ggctgtcggg cgtgccagca gccgcttcag accgataggt
      181 tctcccgtat cggcacagaa tccatagtcc ccttcatcaa tattgcggat ggtcgcctgt
      241 attttactga gaagttttcg ttcccgatcc cgggtacgga gttccaatgc gtactcttct
      301 tcctgtgtgg cacggtcggc aggatcgggg gctgattcgt gttcttggag atgccctgtt
      361 gtaacggagg cgttttcgat gagttcgtct tgcattttta ccagcaattc gcggaaaaaa
      421 gccaaatggt cgtcattcat ataatcgtct tccggcccaa tccaattcaa aatatcttgt
      481 tctgtcagct ttaccataat gtatgccctc tcggttcacc gcaaaaaaac aaagcactac
      541 aaagtggctt tattcctttg atttttataa ggcttattgc ctaaggcgtt tttgaaaacg
      601 cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg atattgccgt
      661 tccatttcct gcgaacggca cacacaggca caaacgccct cacttatggc ttcggcgaca
      721 cgatgccgtc tgaaggcttc cacggcttcg tgggttgtcc cgccttttga cgttacattt
      781 ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggcaaccgc tcctttaaac
      841 gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat ggcggcattt
      901 tgcaatgcgt ccagcagata aaacacataa gccggcccgc tgccgctgat gccggtaatg
      961 ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt catgattcga
     1021 tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg catacatacc ggatacgccc
     1081 agcccgattt ttccaggtgt attaggcata acccggacaa tgcggcggct tcccccgagg
     1141 taacggctga gcgtaccgac cgacaatccg gctgcgacag aaagcaccaa tgcgccgttg
     1201 gtgcggatat ttttgcacgc ggcttccata tcctgcggtt tgacggcaag aatcaaaaca
     1261 tcacccgaat gcaattccgg cagggttgcc gaagtttcga cccccaactc tttttccaaa
     1321 cgttcgcgct tttccgcacc acggttggct atatggatgc ggtatccccc ttgtttgacc
     1381 aatccgcccg caacggcagc cgccatattt ccgccgccga gaaaataaac attcattatt
     1441 tctaccctat tcaaaattac cttactaaat tcattcccaa ttttttacca tcggacgcgc
     1501 tcggacaggc cgttgcgccg caacccctgc ttttccaaac agtcgaaacc ttcctgtttg
     1561 aacctctcaa ccaaaggttt gccgtcacgc gtacgccaat ccaacccgca ggcttcaaac
     1621 ttccctattt tcctataaaa gtaagatttt tgcgcggatt catattcgtt tgccctgacg
     1681 gaacgattgc cgtctgaaag cggtatctcg tagtcctcta aagacagcgg actctcgccg
     1741 ccaatatccc aaaaatcgat ggcggcaggt tttctcggct tacaccagcc ggacaaggac
     1801 gaacactcat accaccccgc cagccggcag cctgcaaccg taaatgcaaa caacgcagcc
     1861 gttgccgtat attttattac ccgaatttcc atcagcccct tttcccgaaa atcgcgctgc
     1921 cgatacggac gtgtgtcgca ccgcactcaa tggcggcagg catatcgtcc gacatcccca
     1981 tagacagcac gtccgcctta acgccagccg cattgaggtc ggcaagcagc ttttgcatgg
     2041 tctgaaactg agttttcaac tccgtttcac tgctgttggc tttggcaacg cacatcagtc
     2101 cgcgcacgac aaggttgggc agcttcgcca cttccacggc aagcgcgacc gcttcttcgg
     2161 gcgcgacacc gtgttttgcc gcctcgcccg caatgttcac ctcgacacac acctgcaagg
     2221 gcggcattaa ggagcgacgc tgcctgctca gccggacggc ggtcttcaga cggcatacgg
     2281 tatgcaccca atgcgcgcgt tcggcgacaa atttggtttt gttggactgc acatcgccga
     2341 tgacgtgcca cacgatgtcg gtcaaatccg ccaattcctc cgttttgccg taccactcct
     2401 gaatatagtt ctcgccgaaa tcacgctgtc cggcagcgta aacttcgcgg atgccgtctg
     2461 aagggaaggt cttgctgacg gcaatcaggc tgacggaatg cggctccctg cccgcctgca
     2521 gaaccaattt tccgatacgg tcggacacct cacgataacg ttcttgcaac accgtcatag
     2581 atcatcccct aattaaaaat gattaaacag ttgaaacccc tccagtcagg gcggtataat
     2641 caaggttgtt agaatcattc caaccaatcg aaacattata ctaaacagag ccgcattatg
     2701 cagattaccg acttactcgc cttcggcgct aaaaacaaag catccgacct tcacctgagt
     2761 tcgggcatat cccctatgat tcgggttcac ggcgacatgc ggcgcatcaa ccttcccgaa
     2821 atgagcgcgg aagaggtcgg caatatggta acttcggtga tgaacgacca ccagcggaaa
     2881 atctaccagc aaaacttgga agtcgacttc tcgttcgaac tgcccaacgt cgcccgattc
     2941 cgcgtcaacg ccttcaacac cggccgcggc cccgccgccg tattccgcac cattcccagc
     3001 accgtcttat cgctggaaga attgaaagcc ccgagcattt tccaaaaaat cgcagaatcg
     3061 ccgcgcggca tggtattggt taccggccct accggttcgg gcaaatcgac cacgcttgcc
     3121 gcgatgatca actacatcaa cgaaacccag ccggcacaca tcctgaccat cgaagacccg
     3181 atcgaattcg tccaccaaag caaaaaatcc ctgattaacc aacgcgagct gcaccagcac
     3241 accctcagct tcgccaacgc gctgagttcc gcattgcgcg aagacccaga cgttatcctt
     3301 gtcggcgaga tgcgcgaccc cgaaaccatc ggcttggcac tgaccgccgc cgaaaccgga
     3361 cacttggttt tcggcacgct gcacacgacc ggcgcggcaa aaaccgtcga ccgtatcgtg
     3421 gacgtattcc ccgccggaga gaaagaaatg gtgcgttcca tgctgtccga atcgctgacc
     3481 gccgtcatct cccaaaacct gctgaaaacg cacgacggca acggccgtgt cgcctcgcac
     3541 gaaatcctga ttgccaaccc cgccgtccgc aacctcatcc gcgaaaacaa aatcacgcag
     3601 attaactccg tcctgcaaac cgggcaggcg agcggtatgc agacgatgga ccaatcgctg
     3661 caatcgctgg tgcgccaagg gctgatcgca ccggaagccg cacgcagacg cgcgcaaaac
     3721 agcgaaagta tgagtttctg acacacaacc gccttccggc catgccggcg ggaaaacaag
     3781 gcgcaaacac gcggggcggg acgcagcatc ccgcccggca tacccttccg aacaggaagc
     3841 gtccgccttc ctgttgaaac ctgccgccgc aaactgcaag gcttaaaccg aaaagaagct
     3901 aacgatgaat accgataacc tgcacgacat cttggacgaa acggttcaag tgtattctca
     3961 aaaaaaacaa agccgatccg aaaccccggc cgaaatcggc acacacttcc acccgctgct
     4021 cgaccgcctg tgcgaaaccg cagaagcaca aaacgcgtcc gacatcctta tcagcaaagg
     4081 attcccgccc tcgttgaaaa tcaacagcgc attaaccccg cagccgcaaa aggctctgac
     4141 gggcgaggaa accgccgcca tcgccgcatc gacgatgaac gccgaacaat cggaaatatt
     4201 ccggcgcgac ggcgaaatca actactccgt ccaatcgcgc agcggcacgc gctaccgcgc
     4261 caacgcctac cacagccaag gcagcgcagg tttggttttg cggcgcatca accacgtcat
     4321 cccgcaaatg cgggaattgg gcttgcccga aaaactcaaa gacctcgccg tcgcaccgcg
     4381 cgggctgctg attatcgtcg ggcctaccgg ttcgggcaaa tccaccacga tggcgactat
     4441 gctcgaacac cgcaacaaaa ccctgcccgg ccatatcgtt accatcgaag acccgattga
     4501 gtttatctac aaaccgcgcc gctgcatctt tacccagcgc gaaatcggcg tcgacaccat
     4561 aaactggcag acggcggtac aaaacgctat gcgccaatcc ccagacgtgg tctgtatcgg
     4621 cgaagtccgc agcagggaaa gtatggaata cgcgatgcag ctcgcccaaa ccggccacct
     4681 gtgcattttt acgctccacg ccaacaccgc gccgcagtcg ctcgaacgca tactcaactt
     4741 ctaccccaaa gaacagcaca accaaatact gattgacatc gccctcaatc tgaccggcat
     4801 catctgccaa cgcctcgccc tcaaaaaaga caaaacgggc aggacggcgg ttgtcgactt
     4861 gctcatcaac acgcccgcca tccaagactt catcctgaag ggcgacctga tgaacatcag
     4921 taaaatcatg gaaaccgcca aaaccgacgg aatgcagacg atggaccaaa accttttcga
     4981 actgtaccgt cacggcatca tcagttacga agaagccctg cgccagtccg tttccgccaa
     5041 caacctgcga ttgcacatcc aactgcacaa agaaggcaaa acgcccgaac tcctttacga
     5101 cagggtcaac ggtctcaacc tcatttcctg atccgcaaaa cccaatgccg tctgaaaacc
     5161 gcatccccgt tttcagacgg catgatttta tccgccccat tcatgtgcca cactttattc
     5221 acttcataac aataaaccgg taaaaccatg aaaaccccac tcctcaagcc tctgctcatt
     5281 acctcgcttc ccgttttcgc cagtgtcttt accgccgcct ccatcgtctg gcagctaggc
     5341 gaacccaagc tcgccatgcc cttcgtactc ggcatcatcg ccggcggcct ggtcgatttg
     5401 gacaaccgcc tgaccggacg gctgaaaaac atcatcgcca ccgtcgccct gtttaccctc
     5461 tcctcgctca cggcgcaaag caccctcggc acagggctgc ccttcatcct cgccatgacc
     5521 ctgatgacct tcggctttac cattttaggc gcggtcgggc tgaaataccg caccttcgcc
     5581 ttcggcgcac tcgccgtcgc cacctacacc acacttacct acacccccga aacctactgg
     5641 ctgaccaacc ccttcatgat tttatgcggc accgtactgt acagcaccgc catcatcctg
     5701 ttccaaatca tcctgcccca ccgccccgtc caagaaagcg tcgccaacgc ctacgaagca
     5761 ctcggcggct acctcgaagc caaagccgac ttcttcgacc ccgatgaggc agcctggata
     5821 ggcaaccgcc acatcgacct cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa
     5881 tgccgttccg ccctgtttta ccgtttgcgc ggcaaacacc gccacccgcg caccgccaaa
     5941 atgctgcgct actacttcgc cgcccaagac atccacgaac gcatcagctc cgcccacgtc
     6001 gactaccaag agatgtccga aaaattcaaa aacaccgaca tcatcttccg catccgccgc
     6061 ctgctcgaaa tgcaggggca ggcgtgccgc aacaccgccc aagccatccg gtcgggcaaa
     6121 gactacacat acagcaaacg cctcggacgc gccatcgaag gctgccgcca atcgctgcgc
     6181 ctcctttcag acggcaacga cagtcccgac atccgccacc tgagccgcct tctcgacaac
     6241 ctcggcagcg tcgaccagca gttccgccaa ctccgacaca gcgactcccc cgccgaaaac
     6301 gaccgcatgg gcgacacccg catcgccgcc ctcgaaaccg gcagcttcaa aaacacctgg
     6361 caggcaatcc gtccgcagct gaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg
     6421 tccctcgtcg ttgccgccgc ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac
     6481 tggatactgc tgaccgccct tttcgtctgc caacccaact acaccgccac caaaagccgc
     6541 gtgtaccaac gcatcgccgg caccgtactc ggcgtaatcg tcggctcgct cgtcccctac
     6601 ttcaccccct ccgtcgaaac caaactctgg atcgtcatcg ccggtaccac cctctttttc
     6661 atgacccgca cctacaaata cagtttttcc accttcttca tcaccattca ggcactgacc
     6721 agcctctccc tcgcaggttt ggacgtatac gccgccatgc ccgtgcgcat catcgacacc
     6781 attatcggcg catcccttgc ctgggcggcg gtcagctacc tgtggccaga ctggaaatac
     6841 ctcacgctcg aacgcaccgc cgcccttgcc gtatgcagca gcggcacata cctccaaaaa
     6901 attgccgaac gcctcaaaac cggcgaaacc ggcgacgaca tagaataccg catcacccgc
     6961 cgccgcgccc acgaacacac cgccgccctc agcagcaccc tttccgacat gagcagcgaa
     7021 cccgcaaaat tcgccgacag cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc
     7081 ctgaccggct acatctccgc cctcggcgca taccgcagcg aaatgcacga agaatgcagc
     7141 cccgacttta ccgcacagtt ccaccttgcc gccgaacaca ccgcccacat cttccaacac
     7201 ctgcccgaca tgggacccga cgactttcag acggcattgg atacactgcg cggcgaactc
     7261 ggcaccctcc gcacccgcag cagcggaaca caaagccaca tcctcctcca acagctccaa
     7321 ctcatcgccc ggcagctcga accctactac cgcgcctacc gacaaattcc gcacaggcag
     7381 ccccaaaacg cagcctgaaa aaatttcggc attttgtaaa ggggggcgga ttgtcaggca
     7441 ggttgcaaga tagtggataa ggctttgcgc agggtaaatg cgtagcaact gaaccgtcat
     7501 tccctcaaaa acagaaaacc aaaaacagaa accta
//
LOCUS       DAXDZG010000086         7451 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXDZG010000086 DAXDZG010000000
VERSION     DAXDZG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.086"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            116..265
                     /locus_tag="ACFB78_001823"
     CDS             116..265
                     /locus_tag="ACFB78_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269763.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            295..3342
                     /locus_tag="ACFB78_001824"
     CDS             295..3342
                     /locus_tag="ACFB78_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFC1269764.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTCASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISESAPILLDTIAEYMGKSRAEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLA
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYRIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3402..3881)
                     /locus_tag="ACFB78_001825"
     CDS             complement(3402..3881)
                     /locus_tag="ACFB78_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC1269765.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4223..5212)
                     /locus_tag="ACFB78_001826"
     CDS             complement(4223..5212)
                     /locus_tag="ACFB78_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1269766.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5472..5660)
                     /locus_tag="ACFB78_001827"
     CDS             complement(5472..5660)
                     /locus_tag="ACFB78_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269767.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5901..6935
                     /gene="purM"
                     /locus_tag="ACFB78_001828"
     CDS             5901..6935
                     /gene="purM"
                     /locus_tag="ACFB78_001828"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC1269768.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
     gene            7153..>7451
                     /locus_tag="ACFB78_001829"
     CDS             7153..>7451
                     /locus_tag="ACFB78_001829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269769.1"
                     /translation="MKQSVHNKTILSVLISSMSVTAFSAPHPPPAINAISVNRTDSGE
                     LIGFEFNNADNTVTGNADITVKPADPSQHSRPKGLIIRSDRNEPNKNQEVRLNNIA"
BASE COUNT         1878 a         1813 c         2046 g         1714 t
ORIGIN      
        1 tatgggcagg cttcgcactc tgaaaggttc aggcggcatt tgtattccct gcgcgtcaaa
       61 ccgccttcgt gtaaagcggt tgcaaaaaag ccttttattt tgacagggtg cagagatgaa
      121 cgccgaagga ttttgtgaaa aacaaaccgc ctgccggccg gacgggcgcc gaaatgcggg
      181 cgatcgtgcc gatttgaagg cctttgaatt tgccggacgg gaattggcga attatcggga
      241 aatgttgaaa cgttatgccg tctgaaaaga cggctttttt atttggggct gtaaatgagc
      301 gatttggaag ccaaagtcag gataacggta gaaaaccata cgaagcaggg ttttgattcg
      361 gccgccgccg atgctgataa ggcggcggaa aagatgcgca gcagcggcga taatgccgca
      421 aagggcttta aagctgcaat cgacagtatg cacgagacca tgcgcaattt gcacgccgat
      481 gtgaaagcgg gatttgaagc ggcgggcaat caggcgcaac aggcgtctga aaaggttagg
      541 gccgaagtgg gtaaaatcgg ctcaggctta tcggggctga ccaaactgct ggcaggatta
      601 gcaaccgcag acttcgccaa atcggtgctt gataccgccg atgcgatgca gtcgataaac
      661 agccaagtcc gacaagtcac gtcgtctgaa acggagtatc tggccgtgca gcagcagctt
      721 ctcgatacgg caaaccgtac ctgtgcatcg cttgaatcaa cggcaaacct gtacgtttcc
      781 acaagccgcg cgttgaaaga ctacggctac acgcagcagg aaattttaaa attcaccgag
      841 gcaaccaaca acgcgatgac catcggcggc gttggcgcgc gacaacaggc cgccgcgctg
      901 ttccagttat cgcaggcttt gggcagcggc gtattgcagg gcgatgaatt taaatccatt
      961 tccgaatccg cacctatttt gctggatacc attgcggaat atatgggcaa atcccgcgct
     1021 gaaatcaaaa agctgggcag cgaagggaaa ttgacggcgg atgtgatttt caaagccatt
     1081 tccggcgcgt cggagaagtt cggggagcag gcggccaaaa tgcccgtgac gatggggcag
     1141 gctttgacgg tgttctcgaa caactggcaa agcatggttt caaaactgct gaacgacagc
     1201 ggcacaatgt cggggattgc cgccgttatt aaactgattg cggataacct gaatttggtc
     1261 gtccctatcg tcgcaggttt tgccgttgcc gttgccgccg ccgttgcacc gacactggct
     1321 ttgaatcttg cactgctggc aaacccgttc gggattattg ccgtcgcaat cggcacggtt
     1381 gtcgggctga ttgcgaaatt cggcgatgaa atcgatgttt tcggcggcgg ctggtcgaat
     1441 ctttccgatg tgattcgggc ggtttggcaa atcatcacgg aaaccgtcgg ggaagcggtg
     1501 ggaaccgtca aatcgtggtt tgacgggctg acaggccggc tgaacgaggg cgcgggcggc
     1561 tggccgtcgt tgttcgggcg cgtgatgagc gtcatctcaa gcgcaatcgg cgcgtatgtg
     1621 aacgtttata tcaacatatt cgcaaccggc tggatgctga ttaaagaagc cgcaaacgat
     1681 atgccgcaat tcttcgccaa tctcggcaag gccataggca acgtgtttat ttccgcgatc
     1741 gaacggatga taaacaaagc ggtaggcatg attaacagca tgattgactt tgccaacaaa
     1801 gccgcgtcga tggccgggat atcgggcatt ggaaagctga acaaggttcg gatggggcgg
     1861 atggacgacg gcgggcttgg cgagcgtatt catgacagct tgacgaaaga ccgcgccggc
     1921 gcaatggcaa atgccgtccg cgaacgggcc gccgatattc acgaagccga tgccctcaag
     1981 ggacgcggcg gcggaggaca cgccaaaacc gcccggaaaa agccgggcgc aaatcagggc
     2041 ggcggcaaag ggggcaaatc ccgttcgggc ggtccgggcg cggccaaaga cccgatgcag
     2101 gcttgggaag gggaaatcaa agcccgaaaa ctcgcacacc gcgaaatgca gcgcgaaacg
     2161 ctcgcacacc aagaatggga tttggcgcgt gaggcggaat attggcgggc gaagctggca
     2221 acggtggacg ccaacggcaa aacgggcgtg aaaatccgcg aaaaaatcct gacgcttgaa
     2281 gaccaattat cgaagcagtc aaccgaagcc aaaatgaatc aggcggccga atgggagaaa
     2341 ttggacaagc acaagctgga gatggagaaa gacgcggcag accaagccct ggcagacgga
     2401 cgtatttcgc aactcgaacg cctggacttg gaaatcgagt ttgaaaaccg acgttaccgg
     2461 attgcctatg acgcattgca ggaacgcatc gcacttgccg aacaagaccc ggcatacagt
     2521 caggcggcaa tcggcaagct caaggcccaa atgggcgaat tggggcgggg gcacgaacgg
     2581 acgcaggcga agaacgaggg caaacgcgaa aaccaacgcc gcaaagacgc gcccaacgtc
     2641 atggaaatgc tgcaagacgg cggcaggaac gtttggcagg aagcacagca gcagatgggg
     2701 caggcgttca ccgccatgct ggcgagggcg cagaacttca ggcaggcgat gaacggtttc
     2761 ttcaaaagta tggggcaaac ctttattcag gaaatggtta cgaaaccttt gacgggcttg
     2821 atgcggcgca tggttgagga atcggccatt tacaagatga ttttcggaac taaggaaacg
     2881 ctggagacag cggcggcagc caagacggcg gcaactaagg caaccgagac aacggcggtt
     2941 gtcagtagta atgctgttca agcggcttca ggcgcggcgg cttcccaggc gcatatccca
     3001 tatgtcggcc cgattcttgc cgttgcggct atggcggcga tgatgtcggc tgtaatggga
     3061 ctgatgggcg gcggcggcgg ttctcaaacg accacaacca cgacgcgcat tccctcggcg
     3121 gcaggcggct gggacattcc ggcaggcatc aacccgctga cccaactgca cgagaacgag
     3181 atggtcttac ccgcagaaca cgcccaaacc atccgtgaaa tggcggatca gcagggaggc
     3241 ggcagcaccg tcatcatcaa ttcgacagga ggcgacttca tccataaaaa cgatttggcg
     3301 aagctcttga agcagatgaa acgggacttt aaatttgtct gagaaaatgc cgtccgaaag
     3361 gttcggacgg cctatgttgc ggcgtacgtc aagctattaa gctatttggt tttcagaggc
     3421 tttttcttcc atttccgggc tacggcttca aattcttgaa aggccgtcgg cgcgctcctg
     3481 atacggcgta tttcaaaaat gaagctgctt aggttgtccc agtcgcgtaa cagcatggag
     3541 tattcgaaat ctttatacat tttctcgtgc agcgccccgc ccaatacgcc cgcacagaca
     3601 aattcgcgct ggtttaaaac tgtcagtatg gcttcacggt ctttcttctt gtccggggta
     3661 tctgatgtat aggtggcgag tatgcatccg tctgtttttg ccagcccgtt tactatggtt
     3721 atggcttctt gaagggcggc attgttacgt tcggccatga tcatgtcgat ggtggcgcgc
     3781 tttttggcgt tttccgtatt cctcatgatg ccgtaagcgg caacaaaaac gccgataaca
     3841 gtcaatatcg gcgttgctat ctgtattagg ttgtcagtca ttgccgtccc agccgtccga
     3901 aaggcggaaa tcacggcttt ccgaataaat atgccccatt gtcatcccct taagctgatg
     3961 ttgagaataa atccttgcgt cggaatatac ggcattgcct gactattttc aagattccgc
     4021 accccgcgga ccggactata caaaaaaacc gccttcgtgt aaagcggttg aagagagcct
     4081 ttcaataaaa atgccgtccg aagggctttc ggacggcatt ttttatagat tcttttttaa
     4141 tgaacctttg aaaccgaaga taattaattt tcctctttat tttcaatacg cagtgtgccc
     4201 tatgttttta aataaaaata ttttaatcca acatttttgc aatatccgcc atgtcggggc
     4261 ggtagtaaac attgagtaaa atccttaaat cccgatgccc gctgattttt gccaagacct
     4321 ccacaggcac tttttttgat aggcgcgtga gggcttcgcg gcgggtgtcg tgaaaatgca
     4381 ggccttgtat gccgcaatta tcccgcgctc gcctgaataa tacatcaagg cttttggcat
     4441 ccaggctaaa cacccatgtg tcatcaattc cgcgcagttg ctctattagt tcggcggctc
     4501 gcaaggataa tggcacgtca cggctgtcgc cgtttttagt tattgggagg tgggcaatac
     4561 gacgccgcat attcacatct gcccatttta ggccgcagat ttcgcctgcc cgcatggcgg
     4621 tttcgatggc aaataggacg gcaacggcaa cccgttgcac cgccattttc ggtttctcat
     4681 taggccggta taggagggcg gcgcaaatat cggcaatttc ctgctcggtt ggccgtcttg
     4741 tccttgcccg gctttttttc ggcttgctga ttttgcgtac agggttttcg cgaagaagtc
     4801 cccactcttt catggcgtgt tcgcagacgg cggataaagt ggttaattcg cgtccgacgc
     4861 tggtggggga tacctcctgc aaccgttgat ctcgccaatc ggcgaagtct tgagggcgca
     4921 ggtcggcaag ccgcaccttt gccaaaggtg tttttagtgc gcgtccaatg cggtatgatt
     4981 cttcccgtgc gccccgcttt gatggtgtaa cctcggataa ataccgccgg ataaggtcgg
     5041 caaagatagt atttcgcggg gcattgcctt ggatgccgtc cataatttgc gactctattc
     5101 gcgccgccca cgccttcgcg tctgatgccc gctcaaatgt ggcggaacgg gaaacgcctt
     5161 tcatacggat ttgtacgcgc catttgccgt tacgttgagt gatggttgcc atatttattc
     5221 gggtgcaatt cgggtgcaaa tataatagag caaaacagat ataaagcggg gcaaaatagg
     5281 gctatattaa atttaatgct ttgatatgta gggataaata gatttaaaaa gacgaaagaa
     5341 gtgcggacgt agaataagcg tagatgcgct ccgtccgcac caataaaaat atataaatca
     5401 tattgttaat gattattggg acaattcggt ggcacaaaat ataagctgtc tgcttttcag
     5461 cgggcggctt tttatctgcc tatgtgtttc aacacacaaa acggcacata aagcaccgcc
     5521 atttgttccg caccgaatcg gaagcgggtg cttccgcccg caatcggatt cgccgcccct
     5581 aatgtcgggg ttttaaccgg aaagaagaaa atattgattt tgatgatggc atcaaaaaaa
     5641 cccttccgcc cgtctgccaa aaggaatggt taagaaaagc aatgcgtctt ttgatggaac
     5701 gcgatatata agaaattgag gggaagggca ggcgggtttc tgcccgttaa tcctgcggac
     5761 ggccccgcca aacccgacac gcgcgggcat atggcggatt gtttacaata ttgtctaaaa
     5821 atctgaacct gcaaacgcga aataaggtag aatacgcccc gttattttac cgtccaatcc
     5881 ccatttacca aggaaaaacg atgagtactt cattgagtta ccgcgatgcg ggcgtcggta
     5941 tcgacgcagg cgaccaactg gtcgaaaaaa tcaaaccgtt tgccaaacgc accatgcgtc
     6001 cggaagtatt gggggatttg ggcggttttg gcgcattggt cgaaatcggc aaaaaatatc
     6061 aaaaccccgt attggtcagc ggtacggacg gcgtgggtac caagctcaaa cttgccttcg
     6121 attgggacaa acacgatacg gtgggcatcg accttgttgc aatgagtgtc aacgatattt
     6181 tggttcaagg agctgaacca ttgtttttct tggattattt tgcctgcggt aaattggatg
     6241 ttccgcgtgc gaccgatgtc atcaaaggca ttgcccaagg ttgcgaagaa tccggctgcg
     6301 ccctgattgg cggggaaact gccgaaatgc cgggtatgta tcccgttggt gaatacgatt
     6361 tggcgggttt tgccgtcggc gtggtggaaa aagagaatgt cattaccggc cgcagcatcg
     6421 gcgcgggcga tgtggtattg ggtttggctt ccaacggcgc acattcaaac ggctattccc
     6481 ttatccgtaa aatcatcgaa cgcgacaatc ccgatctgga tgccgagttt gataatggca
     6541 aaactttacg tgaagccgtt attgcgccga cacgtctgta tgtgaaaccg attcttgccg
     6601 ctttggaaaa atttaccatt aaaggtatgg cgcacattac cggcggcggc attaccgaaa
     6661 acgtgccgcg cgtgttgcct aaaaacacgg ttgcccaaat cgatgccgaa tcgtgggaat
     6721 tgcccaagct cttccaatgg cttcaaaagg cgggcaatgt ggaaacccaa gaaatgtacc
     6781 ggacctttaa ctgcggcatc ggtatggtcg tcatcgttgc tgccgaagat gccgatgcgg
     6841 ttcggtcatt cctgagcggg cagggcgaga cagtgtaccg tttgggttgc atccgagagc
     6901 gtcaagggaa cgagcatcaa acccaagttg cctgacttcc aagcatcaaa tgccgtctga
     6961 atttgtttca gacggcattt ccgcatcttc caaacgcgta tccgcaacac agttctgcaa
     7021 actgatattt tgcccggtat ggcggcttaa acttcttcat catatttcga taatgctata
     7081 aaatgatata atcataaaat attgtttatt cctacatcgt attcatccta ttttccgaaa
     7141 tgtgagcgtt ttatgaagca atccgtccat aataaaacaa ttttaagtgt tttgatttca
     7201 tctatgtctg taacggcttt ttccgccccc cacccccccc ccgcgataaa tgctatttca
     7261 gtcaatcgta ccgattccgg cgaactcatc ggttttgagt ttaataatgc ggacaatacc
     7321 gtaaccggca atgcggatat tacggtcaaa cctgccgacc cttcacagca ttcgcgcccg
     7381 aaagggctga ttatccggag cgaccgtaac gaaccaaaca aaaaccaaga agttcgctta
     7441 aataatatag c
//
LOCUS       DAXDZG010000087         7287 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXDZG010000087 DAXDZG010000000
VERSION     DAXDZG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.087"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..1967)
                     /locus_tag="ACFB78_001830"
     CDS             complement(<1..1967)
                     /locus_tag="ACFB78_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFC1269770.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVA"
     gene            complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB78_001831"
     CDS             complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB78_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC1269771.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(3447..3896)
                     /locus_tag="ACFB78_001832"
     CDS             complement(3447..3896)
                     /locus_tag="ACFB78_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269772.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKSELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(4189..5058)
                     /locus_tag="ACFB78_001833"
     CDS             complement(4189..5058)
                     /locus_tag="ACFB78_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC1269773.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRRAVAALVGRKGIDYSSAYSMVHQRFNVESV
                     EGIPAGKLPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(5339..6499)
                     /locus_tag="ACFB78_001834"
     CDS             complement(5339..6499)
                     /locus_tag="ACFB78_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC1269774.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVAWLVIDSYRFCCDDENFKRKPK"
     gene            complement(6590..6997)
                     /locus_tag="ACFB78_001835"
     CDS             complement(6590..6997)
                     /locus_tag="ACFB78_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269775.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
BASE COUNT         1590 a         2161 c         1738 g         1798 t
ORIGIN      
        1 gcaaccagcc ccgttccttc ccagcctatc aggttgccca taccgtccgc catcgccgtt
       61 tccgttcggg caatggttcg ggcgcgtgcg ttgccgaaag catgggattc tttcaggcgg
      121 cctgccaatt cctgcacact gtcgccgttt cgcatggctt cggctacttg ggcgcgtatc
      181 atttcgcgcg tcccttcggt gatttgccac tcggcggcag gatttcggac aagcccgccg
      241 cccgcccgct tcatgccgac catttcggcg gcgcgttcat gcgcccactt gacggcgcgg
      301 ctgcgaatgt tcgtaaccat accggcggca ggttcaggca ttacgcgcaa caaggcggca
      361 accgccccgt cttccgcaac acgcctgatt atcggctcga ccacatcgga caatcccgac
      421 caatcgccaa aatccaagcc gtttgcaaca acccctgccg cgcggctcaa ttccgccgcc
      481 aaatccccgg cctgccaatc gacggccgca ccctcaatca gcgccgcgat ttgttcggcc
      541 aagccgtcaa tgcgtgtcag caaataagcc tcaataagcg cggcagattc gtcttcgctc
      601 atcgggcttt ccgactttcc caacttttca gccccttggt tcggcggctc ttcgggcttt
      661 cggccgtctt gcttatccgg ttcaggctgc ccctgccccg gtaacggctc cttgcccagt
      721 tcggcgcgga tttcgtcggc ggtcaagatg cctgcgtttt tgtagatggc gtagatttca
      781 gcctgttctt tcgggttgag cgattcctcc cccttccaga caaactcata cgccgccata
      841 tccatgtaac gggcaagcac gtcgtcaatc agggctttta cccagttttt caggctgccc
      901 atgccgtctg aaagcgactg ctcgcggctc gtctctgcca cgctgcggtt tacctgcgcc
      961 acgaacggcg taggctcgac actaaacgca aagcagacga cacgcgccag ccattcgtcg
     1021 taaacgtcct tcaacggcgg ctgcttcgtc tcgcggaagt ttcgggacaa ctcgcccggc
     1081 acgaaacgca ttttgcgccg ctgcgccgtt tcgcccgaca gcagcaaatc ccagtattct
     1141 tgaaaccgcc tgatgtcgtc cgccgaccac gtttcaggca cgccgactaa agcatcgggc
     1201 acgctgcccg ccgtgtagta ttccagcgcg tgaacctgcc gttttagggc gatattcacg
     1261 gtcatgatga tttgctcgac gggcgaatag ccgtaaacct tgtaacttcg gttattccgc
     1321 gaacggtaaa tcaactcgtc agccgtgtaa tcgaccgccg ccatgccgtg caggatttgc
     1381 tgatacgccg tatcgggcgg taacggcata cgccccgtat tgtccaaaac gcgcttaatc
     1441 gtcgccccgt ccatcacttc gagggcgtac aagccgccgc ccagtgtttt gcgcgggtag
     1501 atgcacggcg cgtcaataac aaacaggtct tccagcaaga tgcgcagcca gtccgcccac
     1561 gtatgttctt tatcgggcga ccggaagaac gcaacggctt catcgacctt tcggtctttc
     1621 cgttgcgatt cgtcgtcttc ggttgattcg acgtcgcgct tttggattgt ccacttaagg
     1681 cactccattt ggtctttacg cgcctcgatc accaaacgca atacatcgta gttgtcggca
     1741 agggcgcgta attgcgcaaa gcctaccgct tcacgttcgc gcggcttgga atgcccgacg
     1801 ttgtagaacg gctcgtaatc aaaccgccgc ccctctgcct gctgcgcggc aggggccgga
     1861 ggctcgcccg cgtcgaacca cccgtccgcg ttgccggtaa aggcgtaacg gacgccggcg
     1921 gcaacgcggg caataaagcc ttgcgataaa ggtgtctttt tactcatttc acagcctcga
     1981 cctgcgatcg caggaaatca atcatgcccg tccgggtatc cagcagctcg ccaaacgcac
     2041 ggcccaaaca gtctatttgg tcgtcatgcc ggccgttcgg gaacatgcgc atttccgcaa
     2101 tcagcgcgtc cgtgtcccat gtgccgtcat ccaacaccat cacattgccg atgttgacct
     2161 gtgccgcgaa cggtccggcg cgtgtaacct tgtcgcccga ttcggggccg gcggatacgg
     2221 aaaaacccgc caactggcgg gtcaggtata gtgtttgcga tttgcctgcc tgcccggggt
     2281 cttgcgggat ggatattttc gttttcacgc cgtctttttg cgccgtgttg cgtaatatcc
     2341 tgtcccgctc gtccgcgccg taccggccgc gcacgacgtt ggcgatgata taccgcccgt
     2401 cttccgtaac gccaagcctg ccgcctgccg tgtagtcgcc gccgtttgcg gttgaagcca
     2461 agtcccacgc gcgtacccat ctgatattcc cggcaggcaa cgccttgaca aattgcaggt
     2521 tgtcaggctt gaacgtaccg ccgtcaggcg gcgcaggttt ttgcaaatac tgcccggcaa
     2581 acacatacgg cgcggcctgc tccattcggc gcagtgtttc aatatcatgc ttttcaggcc
     2641 acaacgccgt gccgtcttcc tgaatggcag gcaggcacaa atgctcccac tcttcgccgt
     2701 tgccgccgtc aagcagccag cccgccaagt ctttctcgtg caggcgttgc ataatcagga
     2761 taatcggcgt gtcagggctg ttcttccggg attcgaccgt gttttgaaac cagtcgatga
     2821 cgttctgccg cctgacctcg cttcgcgctt catctgcttt gtgcgggtcg tcaatgatga
     2881 tgcagccgcc gaatccctcc cgatgcctgc ccgcaccgaa acctgtaatc gtaccgcccg
     2941 cacctgttgc gtacatcacg ccgcctgcgg ttgttttcca gtgatggccg ctttcgcctg
     3001 ccagtgccaa atcaggaaaa atcgcccgat actcttcgtg ttgcaccaag ttccgaatct
     3061 gtacggagtt attgaccgcc agcgccgccg aatagctcgc gtggataaac tcgcaatcag
     3121 gcacgcgccc catcgcccac gcgataaagt tcacgaccgc gatttccgtt ttcgagtatc
     3181 gcggcggaat attgataatc aggcgtttcg tttcgccgtt gaaaacacgc tcgagcgcgt
     3241 tgcagattgg cgcatggtga ttcgcccgct gccaaacata gccgcgcctt tgataaaaca
     3301 tccggcgcgt gaacatatac aggcttgcag aacttaaact gcgaattact gatgtttcaa
     3361 catcgtcgaa ttgccctagt gccatttcat tctaacttcc tttggagaaa cggccataca
     3421 gcccctctta tgattagcgt tttatgctaa accctacgca aaacatcttc cgctatcttg
     3481 cgatattcct cagcatcaag ccgtacagcc gccctcatgc tcccatcgcc cgatttaacg
     3541 tcaagctccg atttgtcgcc gtatttcttc ggcgcaatct tggaagccgc ccactttcgg
     3601 gcatctatct gcaatttcgc ctttgaaacc gccgcgctct ctgcttgtgc actatcggca
     3661 atctcgatga tttcttcagc gaaatagtcc gcctgctttt ctctcgcgcg cgcgtattgg
     3721 tggcgaaatt cttcattagc ttccaaccaa cggtaaatag ttttcattgg cggcacacca
     3781 tcttcggcgc atattgaacg taggcttctg ccatttgcga ttttttcgca tatcttatcg
     3841 gccatgtctt tcgtgtaatc tgtcggacgg cctaatttgc gtttcgtgcc gctcataaac
     3901 cctcctcaaa aaagaaaccg tctaactctg acccctctca gaattagacg gcaaacacac
     3961 actcgccaca taggaaaaac ggaacgccgc tacctgtaca ggcagaagct caaattcgga
     4021 cagcctaaaa cgcaaaaacc cgcacattgt tatgtacggg cttaaaaatt catatccttc
     4081 gggcgtgcga aaatccccgc aagggtaacg atttgaatta tacacctatt gccggaaaaa
     4141 acaacaggcc gtctgaagat tcagacggca tttgtattcc ctactgcatc aaaccgccga
     4201 caggttgcgg atttcgggca gtatcgggcg gattttttct gcgtgttccg cgtcggcgtg
     4261 tgcgcttaag gcttcgaggg cgtttgcggc ggctttgagg cggctgcgcg tttccgccca
     4321 gaccgtccac atcgtaaccg cctgcctgca gccgagctgc ttcagcggca gggaaacgtc
     4381 tctgcccatt tggatggccc gcgcgccgta gctgacggcg acggcgaggt cgtacagggc
     4441 gttgccgctt atgggcaggg cgggttgcgg cgcgggtaac ggttcgcggt cgaggacttc
     4501 gcccgtcaag cctgtgtgca gggtcagcgc gtggacgtag gcgacggctt cgggcagctt
     4561 cccggcgggg atgccttcga cggattcgac gttgaagcgt tggtgtacca tactgtacgc
     4621 ggaggagtag tctatgcctt tgcgtccgac aagcgcggca acggcccggc gcaatccggt
     4681 acggtcgtcg gcggtggttt tttgtccgac ttggtagccg cctgttttgc ggatggtggg
     4741 caggacttcg gatgttaccc atttgcggaa tttccaagcg gttgagcctt gttccattgc
     4801 tttacggctg cgaagaatca aaatatataa accgctttcg ttgatgatgt tcacgttacc
     4861 gccgcctccg tgaatttcag accgccctat gttaaacata gaccgctcgt catcatctaa
     4921 tttttcaagt gcttgcgttg ggttctggat ttctaaagcc ttacatacat cggcggcaac
     4981 aaaccaagtc aagccgtctt tttcaaaggc acgaacggga gaagtagtat tgaaattaaa
     5041 tgattgaatt acgttcataa tgaagtttcc tagtgagttt tcttaatgcc cgttagggcg
     5101 gacgcgtggt taagaaccct cactagatgg gcggacttat tccccttacg ggtattgtat
     5161 tcgtcgccca cgcgtccata agaaacttcg gttgtgccat cgaaacaaac aacacgaaag
     5221 gaaacttaca gatatgaaaa aatcacattg acggagtgat tgccgctagt gtgtggttct
     5281 tacgccacga acaggaatat aaaacaaacc ccctgcacat gcaaggggtt ttgttacctt
     5341 atttgggttt gcgtttaaaa ttctcgtcat cacaacagaa tcggtaagaa tcaataacca
     5401 accaagcaac gcgaagcaga ttttcaggcg tatcaataat aacttgattg cctgatacct
     5461 caagtccgca tcgttcgata ttcgaaatat ccgactcttc caattcgata gggaagatta
     5521 cagacggcct ttgtttgttg tcaaaatacc gtaatatcca cctgttactt ttaccatcaa
     5581 ccaatacgcc gaaatagctt tccgtatctt ttgcctcaat actcgcatca tcaggaagaa
     5641 ttgatttaac caaatcaaac aaccttcttt ctgaataagt ggtaacgatt ttattgtttt
     5701 cggggtcgat aatcggcgcg gtcgggtctt cctgctcctt ctcaacaggg gcggcctcct
     5761 cctgcacttt cggcgcactc agtcccgata caaccattga actaaccgta ttttgaacag
     5821 cctgtttgac gatatgacgg atgctttcca aatatcgttg ggtaaactgc cgttgaatat
     5881 tcgcccttcc cgccacataa cggacgaaat ccaaatccac ctctttcaag ctttctgtaa
     5941 ttgattctgt aaatgccgtc aggtatatgc tttcttccgc aaggcttcgc aatgcatcag
     6001 gttgaaactt gtcgtgcctg aactgataca gttgcgccat atcattttca ttcaacatcg
     6061 taacatcaac ggtcaaaaac ggctccgaat ccataatatt cttgttggac aaatcggtaa
     6121 aaaatcgcca ttcccggccg ttggtaatcg cgcaaatggc tatttcaggc gtggcattga
     6181 agtaacgcga caactgcgga caatgattgg acaaattttc agtatatgat tttgcttcga
     6241 taaacataac cggtgcgccg ttgcaaaaca atgcgtaatc gacccgctcc cctgattttg
     6301 cacctgggaa atccgcctgg tattccgccc gaaccttatt cggatcgaat gccgaaaaac
     6361 ctagaatatc caataaaggc aaaatcaaag cctgttttgt cgtctcctcg gtagtacaga
     6421 tatgtgcaac cttcttgaca tgttccgcat gggaagcaat ccgttcttta aataccgcac
     6481 ttacagccgc cgcattcatg ttaatctcct tggttaaatt tgattgggca aattcccacc
     6541 ccattaagaa gtgttaattt tcgcaatcat agcgcaaccg aaaagaaaat caaaccttct
     6601 caaacagcaa atcaaaatca ttccctgccg cctgccgtat cgccctatac cacgcggcca
     6661 agcccaaatc cgtctgcaaa tgcaggggtc cctcgccgcg ccgcctgatt tcagcctgca
     6721 aacgttcctc gtaggctgcc tgcgacttcg caccgatgcc gaacgaaata cggacggcct
     6781 cttgttgcgg cgcatccacc ttcgcccatg cctgcaaagt aagaaacatg gcatcttcgc
     6841 cgtatctcaa gccgatttca ggcttatgcg ggcaagcttc ctcccccata taacggcctt
     6901 cgatactcaa acatctattt aggctgcgcg tatcacggta acgacgctca aaagcacgcg
     6961 ccaagtcgtt cataaattca aattcttgct gattcatagc ttgattaacc ctttttcatg
     7021 caacaaaacc aaagtccgca ttacaccttc cgcgaaggcc gtctgaattt ccccttccgc
     7081 acaatccgcc aagcggttct atacatcaaa actcccaaat gattccaaat tctaccgcag
     7141 cccgcgcctg caccgtttcg gatatggcgg acctcggcag gaacgccgaa ccgcccgcaa
     7201 ttgcggcaaa caatacaacc tatatccgcc actttttgca gatgtgattt ttccgcttcg
     7261 gttttacttt tcatccaatt cccccac
//
LOCUS       DAXDZG010000088         7122 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXDZG010000088 DAXDZG010000000
VERSION     DAXDZG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.088"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..410)
                     /locus_tag="ACFB78_001836"
     CDS             complement(<1..410)
                     /locus_tag="ACFB78_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1269776.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
     gene            complement(628..1548)
                     /gene="truB"
                     /locus_tag="ACFB78_001837"
     CDS             complement(628..1548)
                     /gene="truB"
                     /locus_tag="ACFB78_001837"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC1269777.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDGLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            complement(1606..2130)
                     /locus_tag="ACFB78_001838"
                     /pseudo
     CDS             complement(1606..2130)
                     /locus_tag="ACFB78_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2141..2512)
                     /gene="rbfA"
                     /locus_tag="ACFB78_001839"
     CDS             complement(2141..2512)
                     /gene="rbfA"
                     /locus_tag="ACFB78_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC1269778.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            2695..3939
                     /gene="clpX"
                     /locus_tag="ACFB78_001840"
     CDS             2695..3939
                     /gene="clpX"
                     /locus_tag="ACFB78_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC1269779.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(4046..5239)
                     /locus_tag="ACFB78_001841"
     CDS             complement(4046..5239)
                     /locus_tag="ACFB78_001841"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC1269780.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(5338..5547)
                     /locus_tag="ACFB78_001842"
     CDS             complement(5338..5547)
                     /locus_tag="ACFB78_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269781.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            5644..6144
                     /locus_tag="ACFB78_001843"
     CDS             5644..6144
                     /locus_tag="ACFB78_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269782.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            6229..6783
                     /locus_tag="ACFB78_001844"
     CDS             6229..6783
                     /locus_tag="ACFB78_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269783.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            6797..6997
                     /locus_tag="ACFB78_001845"
     CDS             6797..6997
                     /locus_tag="ACFB78_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269784.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
BASE COUNT         1718 a         1855 c         1856 g         1693 t
ORIGIN      
        1 ttattgcgtt taaaataaat ttcttcgcct tcttcggtgc tttgcccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaattgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgatgct gtgtttgtcc
      181 aagaaaaaac ggaaatcctg caatttaaac accaaaacac cattcacttc cacaaagaaa
      241 tggcgtttta actcatcatt attcaataac aaaccgataa tggacgggtc ggttttttcc
      301 accaaatcca acaggatgtt tttggccaaa atggtttttt cttcgttcgc ccatattttt
      361 tcgtgtgata gcagggcttg ggctaattcg gtttgaatgc ctgtcttcat cgttataaat
      421 cctcatcagt tattttaatg tgccattctt gtgtaacggc aaatggcttt acaaataaat
      481 tataataaaa tcaattcttt gatgtctttg ctgtttccgg gggaagttcg caaagttgcg
      541 ataagcgggg cgggtgtccg agtgtgtcgt gattttacgg aaatatcaac acattattca
      601 aacgcagccc gtatttttaa ccgccgctca ggcggaggat gccgccgtgt tcatcaggcg
      661 caaggctttc atacggcata tttctttttg atattccgcc aaaccgacaa agcggccgtt
      721 ttccgtgtaa acgcgtaccg gcgtatcgga aggcaggtct tcctcgaaac gcggccgttg
      781 cccgcattgg agcatatgga cggcataatc gtttaaaacg gtttggggaa agtgtgaaac
      841 caatacgtcg cagggcagca gcagaccgtc gcgttctgtt tcgtttaaat ttgccaaagc
      901 ttcgagcgtg tggctttggg cgatggtaaa gccggcggtt tcggtgcggc gtagggcggt
      961 caggtgggcg aatgtgccga tgtgtttggc gatgccttcg ctgagggtgc ggatgtaggt
     1021 gcctttgctg caacgcacgc ttatcacggc tttgggcgcg tcaaactcgg caatatcgat
     1081 agagtaaacg gtaatgtcgc gcggtttgcg ttcgataaca atgcctttgc gcgcgtattc
     1141 gtacaacggt ttgccttcgt gcttgagggc ggaaaacatc ggcggcactt ggcggatgtt
     1201 gcctgtcagt gcttggcagg ccgtctgaaa ttcggctaag gaaatatcgg cgcgggcggc
     1261 ggcaatgatt tctccttcgg catcgcccgt gctgctggct tcgccgagtt tcagcgtggc
     1321 ggtgtaggct ttgtcggcat ccagcaggta ttgggcgaac ttggccgctt cgccgaagca
     1381 gacgggcaaa agtccggttg ccaaaggatc gagcacgccg gtatgccctg ctttttcggc
     1441 atgaaacaaa cgccgcgctt tttgcagggc ggtgttgctg gaaaggcctt cgggtttgtc
     1501 gagaagaaga acgccgttga ccgggcgttt ggcgggttta ttggtcatat taattaggga
     1561 atattaatgt tcagaccgtc tgaaagggtt cggacggcct gcgtgtcagc ttaattcttc
     1621 attcaataat tcacgaacag agtgtttttc atcaattaga tcaatgctga ttgcatcaaa
     1681 gccatttcgt tttaatgagt caaaactaat gatgcggcca tcaattaagg ggacttgtcc
     1741 ttcagaattg acgaaatctt catattcatc ttcgtagtct atattgtaat aaagatgaat
     1801 cggaactcgg taagtatatt catccaaatc agttaacgtt ctgttttgct tttgattttg
     1861 gctatatttg tcgcagaaca tagcgttata gccatgttcc gccccatcaa acaatacgat
     1921 ttcatcgccg cttacagctt ctaccgccac aatttttacc ggcgccttat cagtatcggt
     1981 aatcaatcca tcaaaaggct ctccatagta acggatgtgg aaagtatctc taccttggca
     2041 ggataatcaa aagcaacgat gcccgccttc aatttgcagg ttatcactat gtgcataggc
     2101 ggctaaatgt tcgggaatca gattgttcat tctggcagta tcagtcttca accggttttt
     2161 ccgccgccac ttgatcgata agggcggaca ggctcatacc gcgttccaaa gattcgtcgt
     2221 atttgaaatg cagttcgggg attttgaaca gcttgatgcg tttggacaat tcgctgcgga
     2281 ggtgtccgcg cgcgtgttcc agcacttctt ccgtaatttc gcgcgtatct tggttcaaaa
     2341 cggtgtagaa cacggtggcg tggctgtaat cgcgggtaac ttcgacttcg ttgatagtga
     2401 tgaagccggc gcgcgggtct ttcagtccgg tacggacgag ttcggcaagc tcgcgcataa
     2461 tttgttcttt gacacggtct tggcgggcgt agccgcgttg gggttttctc atgaatatcc
     2521 tcagatttgg tgtcagacgg catttctgcc gccggggcgg ttgcgaaagg gcgtattata
     2581 gccgaatcgg cgggatttta aggtaatacg ttgataagca cggatatagt tattatactt
     2641 ggcaacccga acggcgcatg ccgtttctgc aacgcataca ttgtaaagaa agccatgtcc
     2701 aacgaaaacc gtacctgttc cttttgcgga aaatccaaat cacacgtcaa acatttgatt
     2761 gagggcgaaa acgcctttat ctgcgacgaa tgcgtctcaa actgcctcga aatattgtac
     2821 gaaggcgaca acggcggcac gcctccggaa aatgccggag gggagccgga agaatccggc
     2881 aaactgccca cgcccgccga aatcgttgcc aacctcgacg attatgtcat cgggcagggg
     2941 caggcgaaaa aggcgctggc ggttgcggtt tacaaccatt acaaacgcct gcgccacccg
     3001 aaagccgacg gcggtgtcga attgtcgaaa tccaacatcc tgcttatcgg cccgaccgga
     3061 tcgggtaaaa cgctgttggc gcaatctttg gcacgcaaac tggacgtgcc gttcgtgatg
     3121 gcggatgcga ccacgctgac cgaagccggc tatgtcggcg aagatgtcga acaaatcatt
     3181 accaaactgt tgggcaaatg cgatttcgat gtcgaaaaag cccagcacgg cattgtctat
     3241 attgacgaaa ttgacaaaat ttcgcgtaaa agcgacaacc cgtccatcac gcgcgacgtg
     3301 tccggcgaag gcgtgcagca agccttgctg aaactgattg aaggtacggt ggcaagcgtt
     3361 ccgccccaag gcggacgcaa gcatccgaat caggaattta tcaacgttga taccgccaac
     3421 atcctgttta tctgcggcgg cgcgtttgca ggcttggaaa aagtgattcg ccagcgcacc
     3481 gagaaaggtg gtatcggttt cggcgcgtcc gttcacagca aggacgaaaa tgccggcatt
     3541 accaagctgt tcggcatcgt cgaaccggaa gatttaatca aattcggcct gattccggaa
     3601 ttaatcggac gtttgcccgt gattgcaact ttagaagaac tggatgagga cgcgctgatt
     3661 aatattttaa ccgagccgaa aaacgctttg gtcaagcagt atcaagcctt gttcggcata
     3721 gaaaacgtcg ggttggaatt tgaagaaggc gcattgcgtt ccatcgcgcg gcaggcaatg
     3781 gaacgcaaaa ccggcgcgcg cggcctgcgt tccatcgtcg aacgctgcct gctcgatacg
     3841 atgtaccgcc tgcccgattt gcaaggcttg aaaaaagtgg tggtcggcaa ggcagtcatc
     3901 gaagagggca gggaaccgga attggtgttc gagtcctgat ggcaaacaaa accgcttcgg
     3961 aacgttggat cacgtttccg aagcggtttt ttgtttctga tgccgtctga ggccttcccg
     4021 tcttcagacg gcatctgctt tctgcctagg ggcggtttgc ggcggcaaat tccgtcagca
     4081 cgtgttccaa acgccgcaaa ccttccgcaa tatcctcatc gttcaacagc agcgaaggcg
     4141 cgaaacgcaa tacgtccgca cccgcaacta agatcatcac gccgtgtttc aaggaggtag
     4201 cggcgatttc ggatgagcgt ccgcgataag gcgtgtccaa cgcgcagccg agcagcagcc
     4261 ccatcccgcg aacctgtgag aacaagcctg ttttcctgcc caaatccagc aatgccgtct
     4321 gaagtttctg cccctgactg cggacgttgt gcaaggtttc cggcgcgttg atgatgtcga
     4381 aggcgcggct gccgaccgca cacgccatcg ggttgccgcc gaaagtcgag ccgtgcgtgc
     4441 ccggttggaa ggcggcggca atcttttctg tcgccagcat cgcgccgatc ggaaagccgc
     4501 agcccaaggc ttttgccgaa ctcaaaatat cgggaacaac gccgtaatgt tcataggcaa
     4561 acagcctgcc cgtatgcccc atcccggttt gaacttcgtc caaaatcaac aacgcattgt
     4621 gccggtcgca cagacggcgc gccgcctgca aatattccgc agtggcgggc aggatgccgc
     4681 tttcgccctg tatcggctcg atgatgaccg cgcaggtctg ttcgccgacg gcagcttcca
     4741 gcgcggcaat atcgttgaac ggaacgtgcg taatgccttg cggcagcggt gcataatcct
     4801 tactgtattt cggctgtccg ccgacggaca cggtaaacag cgtgcgtccg tggaaactgt
     4861 tgatacaggc gacgatttcg cttttgcctc cgccgaaacg gtcgcgcgca tatttccttg
     4921 ccagctttaa cgccgcctca ttcgcttccg cgcccgagtt gcagaaaaaa accttgtcgg
     4981 caaaactgtg tttcaccaat ttttgcgcca attcctgcgc cggccgcgtc gtatagatat
     5041 tggaaatgtg ccacagcttg tgcatctgcg cgtttaaagc atcgacaagg gcagggtggc
     5101 agtgccccag agcgttaacg gcgatgccgc ctgaaaaatc aatatattca cgcccttccg
     5161 tatcccaaac gcggctgcct gaagcgcgtt cgggaatcat cggggcaaag gcgaaattgg
     5221 gggtcagata gttttgcata attgctcctg attgcggttt tgttcgggat tatgcaccgt
     5281 ttttcaaggc ggataaaggg aaattgccga caatatcggg cagtggtaaa aataaaccta
     5341 aatattttca accggatagc tgaaaaaaac aaaaaaattt cgatataatg cttgaggcgc
     5401 ggcagcgttt tgatttagaa tctccccaat atttttatct tcggacggca tttcatcatg
     5461 caaggcaaaa cgtttacaac cgcttactgc tattggtacc acacagacgt gcggtgcttt
     5521 tttgcgcttg cttgaaatcc atcccatacg caaaacaggc cgctcgaaaa cggggcggct
     5581 tttttgttac cttcacacag gtcgcccgat tcggcaacac acgacagaat caggaaaatg
     5641 accatggatg caatgcccca aaccgccgta caaaacgaaa acctgcacac ttgcagcatt
     5701 attgtcgatg atgcgcagga tttttccccc atgcttctcg atgcagggcg ggacgacacg
     5761 ctcatcagcg aatcgatgat tccgcaaatc cgcaccgttg ccgccctgat agccgccgaa
     5821 cggcacgact tcagccgcag cagccccgcc gagtttaccg atgccgccga tttttttgcc
     5881 gcacggattc tcgtattggg cgtgcgacgc ttccatctgg acgtatcgct gcttcagatt
     5941 ttgaaaaccg caaacaagcg cgcctgccgc tttgccgaaa aacaccgcct gtttttcaca
     6001 cccgcacaag ccgaattgag cctgaacaaa cacaaaaacc gccgcctgct gaccatagaa
     6061 accgaacacg aagtcgaaaa caaaggcaac cctgtcgcca acagcctcgc atttgtccgc
     6121 aaactgcaca ccctacccct gtaaacatca cgatttgccg gcagaatgct tgccaaaatt
     6181 cccgacggcg atacaataca accgattttc cacccgaaca ggacacacat gaaaaaaatc
     6241 atcgcctccg cgcttatcgc aacattcgca ctcaccgcct gccaagacga cacgcaggcg
     6301 cggctcgaac ggcagcagaa acagattgaa gccctgcaac agcagctcgc acagcaggca
     6361 gacgatacgg tttaccaact gactcccgaa gcagtcaaag acaccattcc tgcccaggcg
     6421 caggcaaacg gcaacaacgg tcagcccgtt accggcaaag acgggcagca gtatatttac
     6481 gaccaatcga caggaagctg gctgctgcaa agcctgattg gcgcggcggc aggcgcgttt
     6541 atcggcaacg cgctggcaaa caaattcaca cgggcgggca accaagacag ccccgtcgcc
     6601 cgtcgcgcgc gtgctgccta ccatcagtcc gcacgcccca atgcgcgcac cagcagggat
     6661 ttgaacacgc gcagcctccg tgcaaaacaa caggcggcgc aggcgcagcg ttaccgcccg
     6721 acaacgcgcc cgcccgtcaa ttaccgccgt cccgctatgc gcggtttcgg cagaaggcgg
     6781 taaacccggc gcgccaatgc cgtctgaagg gctttcagac ggcatttttg tatttgttag
     6841 gggcattgtt atgttgccgt ttgattttca gacggcattt tgtttccaag cgtttgatgt
     6901 cgggatggca attctgatct acgtgcgttg gcagggcggc attgtcatac cgccgttggg
     6961 ttttcagacg gcatcatatc gaaccgtcaa gccgtagggc gagattgggt aggcaggaat
     7021 gcccatcctg catctgaaac ccttctgcgt cattcccacg caggaaggaa tctagatatt
     7081 tgaatttacg gaaattttca atcgttgcca aagcggaaaa gg
//
LOCUS       DAXDZG010000089         7062 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXDZG010000089 DAXDZG010000000
VERSION     DAXDZG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.089"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(19..1839)
                     /locus_tag="ACFB78_001846"
     CDS             complement(19..1839)
                     /locus_tag="ACFB78_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC1269785.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKKVLVGKKVLVGKKILVGKNPWEANGARVATYDAEGNEVWVSVPEVV
                     FDEMGFKADAAVIGAEYKAPADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGG
                     KIDPYKHQEDTLAASNTLFMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGD
                     WKQAVSVILKHYPEGVTEGRLEEAFERTRTRCRLKLLKTG"
     gene            complement(2531..2713)
                     /locus_tag="ACFB78_001847"
     CDS             complement(2531..2713)
                     /locus_tag="ACFB78_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269786.1"
                     /translation="MATEILGHTVGVTANELAIHSMEVMDKFSGGEAYNETVWIERGR
                     FADAKRWKGCLSRDAR"
     gene            complement(2750..3034)
                     /locus_tag="ACFB78_001848"
     CDS             complement(2750..3034)
                     /locus_tag="ACFB78_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC1269787.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(3021..3251)
                     /locus_tag="ACFB78_001849"
     CDS             complement(3021..3251)
                     /locus_tag="ACFB78_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC1269788.1"
                     /translation="MEKPLNFKPIPYLQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKLGLKEDADGDE"
     gene            3523..3903
                     /locus_tag="ACFB78_001850"
     CDS             3523..3903
                     /locus_tag="ACFB78_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1269789.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4137..5177
                     /locus_tag="ACFB78_001851"
     CDS             4137..5177
                     /locus_tag="ACFB78_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC1269790.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5174..5548
                     /locus_tag="ACFB78_001852"
     CDS             5174..5548
                     /locus_tag="ACFB78_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269791.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5545..6183
                     /locus_tag="ACFB78_001853"
     CDS             5545..6183
                     /locus_tag="ACFB78_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269792.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6303..6590
                     /locus_tag="ACFB78_001854"
     CDS             6303..6590
                     /locus_tag="ACFB78_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269793.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6664..6885
                     /locus_tag="ACFB78_001855"
     CDS             6664..6885
                     /locus_tag="ACFB78_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269794.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6878..>7062
                     /locus_tag="ACFB78_001856"
     CDS             6878..>7062
                     /locus_tag="ACFB78_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC1269795.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCH"
BASE COUNT         1745 a         1791 c         1639 g         1887 t
ORIGIN      
        1 gctgttttcg gttgaaacct aaccggtttt caaaagtttc agacggcatc ttgtccgggt
       61 gcgttcaaac gcctcttcca gcctgccctc ggtcacacct tccgggtagt gtttgaggat
      121 gaccgacact gcctgtttcc agtcgccgcc gtctgcctcg acgcggggtt ttaagcgttt
      181 ggcgatttcg accttgctca atacctgctc ggcgacctcc atcttgttgt aatccatctg
      241 ttgcccctgt ttgggcataa agagcgtatt gcttgcggca agcgtatctt cttgatgttt
      301 gtacgggtcg attttgccgc cgaacgggac tgccttgcct ttgcgtttgg cggctgccgc
      361 ctccagcgtt tccgcaccca tcgccagctt gtccagctct ttgcgatgcc gttgcgcgtc
      421 tgtatcggcc ggggctttgt attccgcccc gatgaccgcg gcatcagcct tgaagcccat
      481 ctcgtcaaaa actacttcgg gtacggatac ccaaacctcg ttaccctccg cgtcataagt
      541 ggcgacccgc gccccgtttg cctcccaagg attcttaccg accaaaattt tcttaccgac
      601 caaaactttc ttaccgacca aaactttctt accgaccaaa actttcttac cgaccaaaat
      661 ccccttgatg ccttttacgc tatatacccg tccgccaaag cggatttcca aatccgccga
      721 gactttcgcc tctttcggcg cgctgacggc aagctctcgg caatattcgg caggcggcgg
      781 caggatgagc tgctcgggtt tgattttgtt ccacgcctga tagcgggtca tgccgtggcg
      841 gctgtgtttt tgcgtaccgt tgtagtaacg catccagcgt tccgataaag cattgagctg
      901 gtcgatgtcg tgtacctcgg taaagcgcaa cccgctctca aatgccgttt ccacaatatc
      961 gttggctttt tccacttgcc ccttggcacg cggattgccc ggcttgttga tttgcacatg
     1021 cacatccaag ggcttgcaca aatttttaaa cgccgccgaa gtattcgcgc tgcccgggtc
     1081 aagcatgacc atgcgcggta cggtccggaa cgggtctttg ccgatgtctt ttttcgcctg
     1141 catcatgtag atgaaaaaat cacagaggtt cgcgctggtt tcgccgccga agtaataacg
     1201 caccgaaatc gtgccggagg catggtctgt ccccgtgtac cgccaaacgc ggtcgttttc
     1261 gattttgacg acgtttttcg gcttgttttt ataaaactcc tcttccttca tcacccgcag
     1321 ccccgtatcc ttgccctgac gcggcaggaa atacaaaacg cacaaactcg ggtcgatttg
     1381 ccaacaatgg ttcgggtgtt ccgatttcat gcggctgacg gggtcgggct gcaaaagctg
     1441 gtcggaatgc agcttgtatt cccgtaaagc ccgggtaatg gtgttttcag aaagggggat
     1501 gacttccccg gtttccccgt caatccgcgc cgcctcgatt ttcccgttgg cgcgcagcat
     1561 ttccaccgcc tgccgcaccg gcatcaaccg cttgccgttg cgcctcatcg cctccgccaa
     1621 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1681 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1741 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gtcccattgc
     1801 ttccgcctga ttttcgactg ctttaagacg ctcaatcatt gccggattca tcgccttctc
     1861 ccgtttcacc gcccaaccat tccggcacat tgtctgccgg tgcttcggtc ggtagggcat
     1921 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatcttcg
     1981 cgcggtggtc aaatccatgc gcctcgccgt gcgccgccat ctgctcgaac atatcgcgca
     2041 atcggctgat ttgcgagcgg ataccgactt ccaagctgtc aagctgccaa gctgcatcgt
     2101 cagctcgatg cccacatccg caggtttagg ctctttgaca cccgtctgct ttttcgacag
     2161 cttttccgcc agctcatcga cctttttatt tttatcggca atcaccttat ctttcgcttc
     2221 cgccgtttca cggctttcgc gcagggcgac gcgcagctcc ttaatagtca tacggtcgac
     2281 atcgtcaaaa gtgttgccgt tgacctcacc gccttcggca agctccaaca aagtgtcgtc
     2341 gtcttcgacc agcagctcga gcagtttgga tttgcccaac gtcatcagct taggctgcgc
     2401 ttgtttcatt ttcgggtcga taaaacggag ggtggcattc atcagtcttt gggattcccg
     2461 ccgtccgagg ccaactcttt ttcagcgatt tcggcaaacc gcccatgcgg cgtatgctct
     2521 ttgatgatga tcagcgcgcg tcccggctca aacatccctt ccatcgtttg gcgtccgcaa
     2581 atcgtccgcg ctcaatccat accgtctcgt tgtaggcctc gccgcctgaa aacttgtcca
     2641 taacctccat actgtggata gccaattcgt ttgccgttac gccgaccgta tgtcctaaaa
     2701 tttctgttgc catttctttt gctcctcaaa tgcgacgacg tcgtcgcatt tagtaaattc
     2761 ggttttccaa ttcttgcaag cgcgcattca gccgctcttg ctgttttctg aaccgctctg
     2821 cgatttgtag ggttttgatg ccgtaggcgt agttgccgtt ttcaagcttg atgaccaatc
     2881 ccgatgcaac caaatcatca atatccctgc tgacttgtga tggcgtcagc cccaggccgt
     2941 ctgaaagctc cttattgctg atgccgatta ccggatgcgc gtccatcgcc aaaaacaccc
     3001 gcaacagccg ctgcgccttt ttactcgtcg ccatccgcat cctccttcag cccaagtttt
     3061 accgccgctt cgtggctttt gccgaaattg cctttcagct tgccgcgcaa ggggtgttcc
     3121 accgcggtgc gctccaaatt gaaatgtttc gcccggtgcg tcttgcacac gccgttgcgc
     3181 ttaaaccaca cggccgtgct gccgcgcgtt tgcagatagg gaataggctt gaaatttaaa
     3241 ggtttttcaa tattatttat gcctttcgtg tggtataatg tgtttagttt taggcctctt
     3301 tgcacgataa acatccggtc tgttgtgcag cagcagggtc ttggcttgtt ttaaaataaa
     3361 caagcttttt cccgtagccg ggtgtatgtg atccatcatg agctgtcgcg catgagcctt
     3421 caaagtgttc attttttcgt cctttttcgt gatgatttag ggtgtttcat gtcttgatgt
     3481 gtgaattatg agaaaaatat ttctcttttc aaggaaattt aagtggaaaa aacttctctt
     3541 tttggtaagc gattgaaaga agaaagaatt aaattaggct tgaaccaagc agaagctgct
     3601 gaaaaatgtg gtttttctcg tgagatgtgg ggaaagtggg agcgtggtga aaatcgccca
     3661 tcaagtgaaa aattattctc tttttctaaa atcggcatcg atattgatta cgtcatgcac
     3721 ggcagacgcg gcgaaacagc cgccatgccg tctgaatcct tgagtgctga agaaaaagaa
     3781 ctgcttgccc tgttccgtca gctcggcagc ggcagccgca aagagcttgc tgactacgcc
     3841 gcctttaagc tggtggtaga gaaaaaagcc caaactgcgc ttggtaaagt gagcaacgga
     3901 taaaaaatgc cgtctgaaga tttcagacgg catttgggac actctgacag tcgggctgct
     3961 ccggtgttct gaaacagata ttaagcaata ggagcggccg tgcattttaa agcgtattaa
     4021 aaaccacccc gatattcagg tcatcggcca agtggttcag atttcaaaag accttaatta
     4081 acttacaata ggaaaaatga tgacagaaac gaattactct tttgaagggc tggtaattga
     4141 aaaaaattat tacccatcgt atttatccga aaaatgaaaa tagagagcgt gtagaaccta
     4201 aaatcagtac acatttgatt gatttgccta ttacggcaag aagaacattg gaaaccaggc
     4261 tgaccaaagc tttgggtaat aaatctcatg gcattgaaat gtctgttgtc aatacggctg
     4321 aaaatagttt ttttcagata gcggcagcca tacaactcaa agaggaagca gagtttattg
     4381 aggattccgc acaatttgcc cacatgttga ccgatgcaca actgaataca aatgcaccgg
     4441 gtggtatttt gttggtttta aaaggtaggg ttggagatac cggtaagccg tttttatgtg
     4501 taattaaggc tgaacctcaa gatgggttcc gaaccaaaga agaggatgac tttatcacga
     4561 ttgaattctt agaagaatta ttactgaccg attcagcaag attattcaag ataggttttt
     4621 tggtggctga aacagtaagg ccgctagagc aaatacaatc tgggaattat cgagcttttt
     4681 tgtatgacca tctgatgaca caaacggaaa ctagaccggc agcttcctat ttctatcaag
     4741 tattcttggg tatgagtata gctgcttctt cccgtaaatt gacgcaggat ttttttgagt
     4801 ggacacgcaa ttttatcgat aactctgatt taagtgatga tgcaaaatta gatgcgcatg
     4861 aagcattgcg cgttacattg aaaagtgcgg aagcaaccat tagtgtaaat aattttgccc
     4921 aaaatcattt acctcaagaa aaacgaacaa cttatacaga atttatggtg gaaaaggatt
     4981 ttcctcaaaa tgccgtaagt aaagatattg aatatattaa aactcgttta cgcaaacgga
     5041 ggtcttacgg atttagtaac ggtgtagtta tcttgactcc tcccgagcat actcaggact
     5101 atatggaaat tgcgccaacg gaagatgggg aatatactgt tgtcctaatt aaaggacagt
     5161 tacaacaaca aaaatgagtg atattgatga tttcaaaagc tatttagaaa gccaccaagc
     5221 ggcattttcc gcttggggta gatttgtggc tgaagaaatt caaaatcaat tatccaatgt
     5281 catttctcct gttccggttg ccaattttct aaaaattgaa gcaaagcctg gagtcaaaga
     5341 gatttcttca gctctagcca agattggccg aaaaaattac acttcacctc aaactcaaat
     5401 gactgacttg gtcggcgtac gttttgtggc attgcttgcg gaacatattc aaatagtttg
     5461 tgaaatcatt gaatcctcat ctcagtggaa tgctaaagtt tcgaaagatt ttgcagatga
     5521 aattcagcaa aatcccaaag caatttgact atacaatcca aacattacga gattcgtcct
     5581 aaacaagcat tcattacacc cgaaaatgtc agcatccctg ctgatttgtg ctgtgaagtt
     5641 caggtgcgtt ctttactcca acatgcttat gcagagctgg ttcatgacaa tatatacaag
     5701 cctgacggca atgttccaaa gcaagcagag cgcgaagtcg cgaagagcat ggctttgatg
     5761 gaaactacag atgacttatt tagtcgtact ctggcaattt tgaaagaagc aaatcaacca
     5821 caagaagagc ttcttcccca attatcacag ctttatcaaa aggaaatagg gcttgttcct
     5881 gaagttgata aaaaaaccaa tatgattttt ctagaaacat ttcaatccag catttcccaa
     5941 tcaagtattc tttctgacat ccgttcactt ttgaatgaga agaaatatat tgccaagcgg
     6001 ataaaagaaa atgctgaaga aatgtatttt ttcagtcagc cagcagcttt gttggtctat
     6061 tggctgattg agaaagttgg tgcagatgaa gtatggaaaa aatggccttt gcctgcatac
     6121 aacaaaaacc tgaaattcat ttgtactgat ttggataagc aaccatccca tgaactcttt
     6181 taaccccctt taaaagccca ttcagacggc ctttcctaat tatgccttaa tcaaacgcgg
     6241 catagcggca gttccgcagc tggcggaaat caaaaaatcc gcaaatcagg aagggggcgg
     6301 caatgattga atttgtccga gccaaaaaac ggctgctttg ggcatttgta cttttgcttg
     6361 cgtgggcgtg cggctaccgg tacgctgccg acaaggccga agcggaacaa accgccctga
     6421 ttgccgccta tcggcattct tctatggttg cggcggaaca atacgccttg cagcttaaaa
     6481 aagcgcagga cgaaaggcag cggtggtacg atttttccca aaaacaaagc acggatttgg
     6541 cggccgccct gagcgaattg gacaaaacgc gcaacacctt acaggagtaa acccgtgaag
     6601 cggcagacaa agacggcaac cgttttaacg gccttggcac gaacagcctg cacctctaca
     6661 accgtgcctt cggatacccc gattaaaacc gttgccgtcg cggaaattcc gcccgttcct
     6721 tccggactgc tggtcgaata cgaacgcccc gagcgtccgg ccggcggctc tcccgaacaa
     6781 cttttaaacc acgccgtccg ctatggcgga tactaccgaa agctcgaaat ccaaattgag
     6841 ggctggcaga actggcacac gaaaggccgt ctgaaacatg actgattttg ccgaccgcgc
     6901 atccgaacgc gaagccgaat ttctggtgga agccttggcg aaacaccaac cgccgtctga
     6961 aaacaccgcc ggcttcagcc attgcgaaga ctgcggcgac ccgataccgg aagcaaagcg
     7021 aaaagccgtc cgaggctgta cgcgctgtgt tgtctgccac ga
//
LOCUS       DAXDZG010000090         6914 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXDZG010000090 DAXDZG010000000
VERSION     DAXDZG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.090"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..90
                     /locus_tag="ACFB78_001857"
     CDS             <1..90
                     /locus_tag="ACFB78_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC1269796.1"
                     /translation="PPLLRECEFRLNFGTPPRQLKILRDRCGI"
     gene            complement(1068..1751)
                     /locus_tag="ACFB78_001858"
     CDS             complement(1068..1751)
                     /locus_tag="ACFB78_001858"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC1269797.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1900..4203
                     /gene="parC"
                     /locus_tag="ACFB78_001859"
     CDS             1900..4203
                     /gene="parC"
                     /locus_tag="ACFB78_001859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC1269798.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDRSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4223..5947
                     /locus_tag="ACFB78_001860"
     CDS             4223..5947
                     /locus_tag="ACFB78_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC1269799.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            6047..6799
                     /locus_tag="ACFB78_001861"
     CDS             6047..6799
                     /locus_tag="ACFB78_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1269800.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1654 a         2067 c         1703 g         1490 t
ORIGIN      
        1 ccgccgttgt tgagggaatg cgaatttcga cttaacttcg gcacaccgcc ccggcagcta
       61 aaaatcctgc gggatcggtg tggaatttag ggataatcta gtacagcccc tactatttta
      121 gtagttttac cattagaaaa aggatatgaa tggaattgcc ttgaggtaga agtactatcc
      181 gctgctcaat ctctagcaaa tatctattgc aacaaggttc gcctcataga tggtcatgtt
      241 tgggaggaat aaatgaaaaa gcgtagttgg taatttttag ttgcgcaggg tgggcttcag
      301 cccaccgttt tttcttcatt ttgggttgtt gattggtggg ctaaagccca ccctacgatt
      361 actaaaatcc cgtcattccc gcgtagagcg catcataata aggcgggaat ccagaccttt
      421 cggtgcgaaa acttatcgga taaaacggtt tcccctgcat cctggattcc cactttcgtg
      481 ggaatgacgg gatataggtt tccctacgga cgtgttcaga ttcctgcctt cgcgggaata
      541 acggcggagc agtttctgct tttccaataa atgaccgtaa cttaaaatcc cgtcatcccc
      601 gcgaaagcaa aaatcctgcc tgtcggattt cggttttttc gggaaactga tgaatcgtca
      661 ttcccgcgca ggcgggaatc cagaacgcgg gacggcggca atattcaaag gttgtctgaa
      721 aattcagagg ttccagattc ccgccttcgc gggaatgaca gcggagcagt ttctgctttt
      781 ccgataaatg accgcaacct aaaacccatc cttcacacga aaacagaaaa acaaaaacct
      841 aaaatcccgt cattcccgcg ataacagttg cgtaattgcg tagggtgggc ttcagcccac
      901 cgtttcttca tttttgcttg tcaattagtg gactgaagcc caccctgcgg ttactgtttc
      961 ttctttttcg attattaatt ggcaggctga agccctccct acgtttaccg tcaaaaatgc
     1021 cgtctgaaat gctcaagccc tcagacggca ttttttattt cgcaccctta caggtaaaat
     1081 ttctcgatga ctttcaggtt gtcgtctaat ttgtacacca gcggctggcc ggtcgggatt
     1141 tccaagccca tgatgtcttc gtcggaaatg ccctcgatgt gttttgccag cgcgcgcagg
     1201 gagttgccgt gcgccgctac caagacgcgt ttgccgctca aaatcgcggg ggcgatttgg
     1261 tcttcccaaa acggtaatac gcgctccagc gttactttca ggttttcgcc gtcgggtacg
     1321 acatcggcag gcagatgggc atagcgacgg tctttgtgtg cggaaaacgc gtcgtctttg
     1381 tccaaaagcg gcggcagggt gtcgtagctg cgccgccaga tgcggacttg ctcgtcgccg
     1441 tatttttcgg cggtttgttt tttgtccaaa ccttgcagtc ggccgtagtg gcgttcgttc
     1501 agcctccatg ttttgatttg cggtacgaac agttggtcgg attcttccaa aacgatgttg
     1561 caggtcttaa tcgcgcgggt caggacggat gtgaaggcga tgtcgaactc atagccgttt
     1621 tctttcagtt ttttaccggc ggcggcggcc tcggcaagcc cctgctcgct cagcttcacg
     1681 tcgcgccagc ctgtaaacag gtttttcgcg ttccattcgc tttgtccgtg gcggataaat
     1741 accaattcca tatcgtctcc aatgtgtgaa agtgggaaag ctttatttat aacatatttt
     1801 cacatttccc gtatttgatt cagattcagg cacggcgccc actatggttt gccattttga
     1861 tttacaataa tgtcctttgc tttacattcc gcatacacaa tgaatacgca accgcacgct
     1921 tcccataccg attccaacac gctgatgctc ggccgatacg ccgaacgcgc ctatctcgaa
     1981 tacgccatga gcgtggtcaa aggccgcgcg ctgcctgaag tttcagacgg ccaaaagccc
     2041 gtgcagcggc gcattttgtt tgccatgcgc gatatgggtt tgacggcggg ggcgaagccg
     2101 gtgaaatcgg cgcgcgtggt cggcgagatt ttgggtaaat accatccgca cggcgaccgt
     2161 tccgcctatg aggcgatggt gcgcatggct caggatttta ccttgcgcta ccccttaatc
     2221 gacggcatcg gcaacttcgg ttcgcgcgac ggcgacgggg cggcggcgat gcgttacacc
     2281 gaagcgcggc tgacgccgat tgcggaattg ctgttgtccg aaatcaatca ggggacggtg
     2341 gattttatgc cgaactacga cggcgcgttt gacgagccgc tgcaccttcc cgcccgcttg
     2401 cctatggtgt tgctcaacgg cgcgtcgggc atcgcggtgg gtatggcgac cgagattccg
     2461 tcgcacaatt tgaacgaagt cacgcaggcg gcgattgcac tgttgaagaa accgacgctg
     2521 gaaaccgccg acctgatgca atatattcct gctcccgatt ttgccggcgg cggtcaaatc
     2581 atcacgccgg cggacgaatt gcgccgtatt tacgaaaccg gcaagggcag cgtgcgcgtg
     2641 cgtgcgcgtt atgaaatcga gaaattggcg cgcggacagt ggcgcgtcat cgtaaccgaa
     2701 ctgccgccga acgccaattc cgccaaaatc cttgccgaaa tcgaagagca aaccaacccg
     2761 aaaccgaaag cgggcaagaa gcagctcaac caagaccggc ttaataccaa aaagctgatg
     2821 ctggatttaa tcgaccgcgt gcgcgacgag tccgacggcg aacatcccgt gcgccttgta
     2881 tttgaaccca aatccagccg catcgatacc gataccttca tcaacacgct gatggcgcaa
     2941 acttcgctgg aaggcaatgt gtccatgaac ttggtgatga tgggtttgga caaccgcccc
     3001 gcgcagaaaa acctgaaaac gattttgcag gaatggctgg atttccgcgt cgttaccgta
     3061 acacgccgtc tgaaattccg tttaaaccaa gtggaaaaac ggctgcacat cctcgaaggc
     3121 cgtctgaaag tctttctgca catcgacgaa gtgattaaag tcatccgcga atcggacgac
     3181 ccgaaagccg atttgatggc ggcattcggg ctgaccgaaa tccaggccga agacattttg
     3241 gaaatccgcc tgcgtcagct ggcgcgtttg gaaggtttca aactcgaaaa agaattgaac
     3301 gaattgcgcg aagaacaagg ccgtctgaac atccttttgg gcgacgaaaa cgaaaaacgc
     3361 aagctgatta tcaaagagat gcaggcggac atgaagcagt tcggcgacgc gcgccgcacg
     3421 ctggtggaag aggccggacg cgccgtgctg acacaaaccg ccgccgacga acccatcacg
     3481 ctgattttgt cggaaaaagg ctggatacgc agccgtgccg gacataatct cgatttgagc
     3541 caaaccgcgt tcaaagaagg cgaccgcctc aaacaaaccc ttgaaggccg cactgtttta
     3601 cccgtcgtca tcctcgattc atcgggcaga acctactcga tcgatgccgc cgaaatcccc
     3661 ggcggacgcg gcgacggcgt accggtttcc tccttaatcg agttgcaaaa cggcgcgaaa
     3721 cccgtcgcga tgttgacagg attgccggaa caacattatt tattatcaag cagcggcggc
     3781 tacggcttta tcgccaagct gggcgatatg gtcggacgcg tgaaagcggg caaagtggtg
     3841 atgaccgcag acagcggcga aaccgtcctg ccgccggttg ccgtctatgc ctcctcgttc
     3901 atcaaccccg actgcaaaat cattgcagcc accagtcaaa accgcgccct cgccttcccc
     3961 atcggcgaat tgaaaattat ggcgaaaggc aaaggactgc aaatcatcgg attaaacgcc
     4021 ggcgaatcga tgacgcatac cgccgtttct tccgagccgg aaatcctgat tgaaagcgaa
     4081 ggcaggcgcg gcgcggcgca caaagaccgc ctccccgtcg ccctgattga ggcaaaacgc
     4141 ggcaaaaaag gcagactgtt gcccatatcg ggcagcctga aacagctttc ttcccccaaa
     4201 taaacccggt tccgcacata ttatggtgat ttccaacccc cgcgaacttg aaaaactcaa
     4261 agaccggatt cccaatctga tcaacatcat ccgcgtcgcc atcgttttcc cgctgatgat
     4321 tatgcacatc ctcgggctgg aaaccggcag ccgtgcgaac ctgcacgcct catggacggc
     4381 gtgggcgttt tacttgtggc ttgccattgc ctgctggctg attttctttt ccaccctcaa
     4441 cccgcaatgg caatggcaat ggcaggcttt gagaataccg agtttcagcg ccgtggccga
     4501 catcacgatg atcggcgtgc tgacctacct gttcggcggc atcgattcgg ggttcggcat
     4561 cctgatcctg cccttcgtgg gcagctcctg cctactcagt tacgggcgtt atcccctgct
     4621 ctatgccagc tatgcatcca tcctgctgat attcaacgcc cttgcagaca gcaatatcaa
     4681 tatgtatccg ctgattctgg atgcaaaaac cgtcaccaat accttcgtcg tcgtagccgg
     4741 ctcctatttc gtcgctatga ttgcctcgct gtcggtcaga tacatcgacc gtgccggcaa
     4801 acttgcccat gaaaaccacg tcgcctaccg ccgtatcagg ggcttgaacc aaatcgtgct
     4861 caaccgcgtt caggaagcgg tcgtcgtcat caacgtcgag catcagacca tactgttcaa
     4921 taaaaaggca aaagatctgc tccccatgct tgaaatcgga cagcatacct ccctgttcga
     4981 ccctgtcgcc atcttatggg ataaaacatc ctcacgcacc ttcgaacact atatcgacac
     5041 gcccgaactg accgcccgca tccgcgccgt gccgatgaac aaaaagcaga acaagctgct
     5101 catcctctat atccgcccgc aaagcgaaat tcaggcggaa gccctgtccg tcaaacttgc
     5161 cgcgctcgga caactgaccg ccaaccttgc ccacgaaatc cgcaacccga tgtccgccat
     5221 ccgccacgcc gacggcggca ctttattttt ggacgaagtg gccgacctgc ccctttccat
     5281 gcaggtcaaa ctcttgcgcg cgattcaaga aaaagccgtg cgccgtatcg gcgacgcgac
     5341 cgagcagccc gtcgatgtcc gcatcgtctg cgccacccac aaaaacctcg aagcccttgt
     5401 cgaaagcggc gcattccgcc aagacctgta ttaccgtctc aatgtcgtca gcctcaatat
     5461 gccgtccctg cgtgaaatgc gcgaaaattt gaagctgccc accccctacc tcctgtacaa
     5521 acacagccac aacaaccggc cctacacact ctcccccgcc gcgcaacaga tgctcctgaa
     5581 ttacagttat ccgggcaatt tccgcgaact cgaaaacatc ctcgaacgcg ccgtcgccct
     5641 gtgcgtcgga tatacagtgc aaatcgacga cctgcaaatc caagatgtgc accacaaacc
     5701 cgtccggacg gaaaccgccg tccccgttgc cgataccttg ccgtctgaaa cagccgccgc
     5761 accgtcccgc cttctcccgt tcgaccccga taccatgcag atacaggact atctcgacaa
     5821 aatcgaacgc gacatcatcg gacaagtcct caaacaaacc gaaggcaatc gcacgcaagc
     5881 cgccaaacgc ttgggcatca gcttccgctc tatgcgctac cgtatggaac gcctcaacat
     5941 agactgacaa acggcatccg caccatctcc gcccacccga aaaaatgccg tctgaaacgg
     6001 cacggggaag cgggttcgcc ccacgaccga acggacacaa tacaccatga ccgacatcct
     6061 cattgacgat accgccaccg aagccgtccg caccctgata cgggcattcc ccctcgtgcc
     6121 cgtttcccaa ccgcccgaac aaggcagtta cctccttgct gaacacgata ccgtcagcct
     6181 caggcttgtc ggggaaaaaa gcaacgtcat cgtcgatttt acctccggcg cggcacaata
     6241 ccggcgcaca aaaggcggag gagaactcat cgccaaagcc gtcaaccaca ccgcgcaccc
     6301 caccgtatgg gatgcaaccg caggattggg gcgcgacagc ttcgtcctcg cctcgctcgg
     6361 actgaccgtt accgccttcg agcaacatcc cgccgtcgcc tgcctgcttt cagacggcat
     6421 ccgccgcgcc ctcctcaatc ccgaaacgca agacaccgcc gcgcgcatca acctccattt
     6481 cggcaatgcc gccgaacaaa tgcctgccct tgttaaaaca caaggcaaac ccgacatcgt
     6541 ttatctcgac ccgatgtatc ccgaacgccg caaaagtgcc gccgtcaaaa aagaaatggc
     6601 ttatttccac cggcttgtcg gcgaagcgca agatgaggtc gtcctccttc ataccgcacg
     6661 ccaaacggca aaaaaacgcg tcgtcgtcaa acgcccgcgc ctcggcgaac accttgccgg
     6721 acaagccccc gcctaccaat acaccggcaa aagcacccgc ttcgacgttt acctgcccta
     6781 cggagcggac aagggataac gcccataaaa caagacaccg aaaaatttgc cgttcttatg
     6841 caaacgagaa accggttttt gcgtttcgac tgttttggat aagtcatcac accttaaagt
     6901 ttgtcattcc cacg
//
LOCUS       DAXDZG010000091         6835 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXDZG010000091 DAXDZG010000000
VERSION     DAXDZG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.091"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            29..241
                     /locus_tag="ACFB78_001862"
     CDS             29..241
                     /locus_tag="ACFB78_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC1269801.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            426..936
                     /locus_tag="ACFB78_001863"
                     /pseudo
     CDS             426..936
                     /locus_tag="ACFB78_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            933..1259
                     /locus_tag="ACFB78_001864"
     CDS             933..1259
                     /locus_tag="ACFB78_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC1269802.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAVGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1280..1990
                     /locus_tag="ACFB78_001865"
                     /pseudo
     CDS             1280..1990
                     /locus_tag="ACFB78_001865"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            2166..2714
                     /locus_tag="ACFB78_001866"
     CDS             2166..2714
                     /locus_tag="ACFB78_001866"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC1269803.1"
                     /translation="MNSTVPANNSPLQHALAKLTERETAAVSRQLDPGFLPFHAFARS
                     IGTEEGWDFAETDEARRNLIAGFAEIHARKGTPYAIRALFPILRLGEIQIIERDGEFK
                     WDGSVLFDGSRTFGRRKGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCELTALDY
                     RNHPHRWNGKIRFNGEYGFGTT"
     gene            2754..4957
                     /locus_tag="ACFB78_001867"
                     /pseudo
     CDS             2754..4957
                     /locus_tag="ACFB78_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5056..5385
                     /locus_tag="ACFB78_001868"
     CDS             5056..5385
                     /locus_tag="ACFB78_001868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269804.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5401..5712)
                     /locus_tag="ACFB78_001869"
     CDS             complement(5401..5712)
                     /locus_tag="ACFB78_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC1269805.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5720..5999)
                     /locus_tag="ACFB78_001870"
                     /pseudo
     CDS             complement(5720..5999)
                     /locus_tag="ACFB78_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6084..6697
                     /locus_tag="ACFB78_001871"
                     /pseudo
     CDS             6084..6697
                     /locus_tag="ACFB78_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6676..>6835
                     /locus_tag="ACFB78_001872"
     CDS             6676..>6835
                     /locus_tag="ACFB78_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC1269806.1"
                     /translation="MGAKRRLREYIYHLLVAIDQLFNAITGGAADETLSSRTYRGARL
                     AQQPKTRWK"
BASE COUNT         1852 a         2337 c         1632 g         1014 t
ORIGIN      
        1 tatgcgaaac aaaataaacc gcaacgacat ggaactcggc tacacgcctt acaaccttcg
       61 caccctgcgc aaccgctgca aactgacaca ggccgagttg gcgcaaatcg tcggggtgaa
      121 gcactacata caggtaggcc gctgggaagc cgaacccgat accgaaacgc ggcgggccga
      181 tatgccgctg gaaaaatggc ggcagttcct cgattggata gaaaaaacaa acgccgtctg
      241 aaaccttcag acggcatttt ttacccaccc ccaaacctaa aaaccacaac caagccctac
      301 accgcttgcc cccacctcgc cgccgcccca aaataacctc actctgttgg aagcctgccg
      361 gaaacggcaa aagccgcccc gttccgccat tacggggcgg tttcccacat caagacgaga
      421 cagaaatgac cgaccgccaa atagacaacc tcatcaaacc cgccaccatc gccgacaccg
      481 accccgcgtc caaccgcgtc cgcgcacaac acggcggcat taccaccgac tggctgccct
      541 atatcgtccc ctttgcaggc ggcgtgtcag tatggcgcat cccaagtgtc ggcgaagcct
      601 gcaccatctt atcccctgcc ggcgaacccg agaacggcgt agttttatgc tgccaagcct
      661 ccgaccgata ccccgccccg tccgccgacc ccgccgaaac cgtcgtccga tttcatccgc
      721 tacaaccaca acagcggcgc gatggaatta aaagccgtta caagcctgac catcgacacc
      781 ccccaaacca ccataaccgg acacctgacc gtcaaccaaa ccaccaccgc ccaagggctg
      841 ctgacctacc aaaatggcat gaacggccaa ggcggcagcc tgtccgagca cacccacccc
      901 gacgactccg gcggtacaac ggaaaaaccc caatgaccga cgcagaaaac ggacgcgggc
      961 aagacaccct cgcccacatc gcccaatcca tccgcaacat cctgttcaca agaataggaa
     1021 cgcggctgat gcgcgaagaa tacggcagtt ttattcccga cttgatcgat atgcccgccg
     1081 gtcacgccgc catcgccctc atccaccaag ccgccgttac cgccctcgca agatggaaac
     1141 cccgcataac cgtccgccgc atacaggcag acaccgccga cctcgccgtc ggcaaaatca
     1201 aattaaccct agacgtaacc cttgccgacg gcggcgaacg aacctaccgc atcaaataat
     1261 aaaaacaaca ggataaacaa tgggaaacag ccgattaagc caactccccg cgcccgccgc
     1321 catcgaagaa accgactttg agggcatctt cgcgcgcaaa aaagccgccc tcaccgccct
     1381 atgtcccgaa agcatccgcg aaaccgtcgc ccaaaccctc gaattagaat cagaacccct
     1441 aaccatcgac ctgcaacagc aagcctatca agagctgctc gtccgcaacc gcatcaacga
     1501 agccgtcaaa gccaacctct tggcatacgc acaaggcagc gacctcgacc acatcgccgc
     1561 ccaatacgga ctttcacgca aaaccatccg cgccgccgac cccgacgcga acccgccggt
     1621 tgccgccgaa tacgaaaccg acgacgcatt ccgcgcccgc gtccaagccc atcccgaaaa
     1681 atacgccgcc gggccgcgca ccgcatacga agcccacgcc atcgacgcac acccccaaat
     1741 cacacacgcc cgcgccgtgc gccgcgccgc cggcacggtg gaggtttaca tcaaaaccca
     1801 aagcggcacg cccgacgaaa ccattttgac cgccgcgcgc gaatacctgt ccgccgaaac
     1861 acgccgcccc ctttgcgaca acgtgcaagt aaccgccgcc caacccaaag acgcggcagt
     1921 agagtattcc gccgaatacc accccgccgc caatattcaa gccgaacggc agaccgcctg
     1981 cgaagccttg gataacctat ggcggcaaaa cgcccacatc ggcgcatccg tcgccctgtc
     2041 taaaatcatc ggcgcgttag acacccccgg cgtgaaaaaa aatcacactg cacagccccg
     2101 ctgccgacat cgaatgcggc aacggcgaat acatccgaat cacgtccacg ctcgagcgcg
     2161 aataaatgaa cagcaccgtc cccgcgaaca acagccccct gcaacacgca ctggcaaagc
     2221 tgacagaacg cgaaactgcc gccgtctccc gccaactcga ccccggattt ttaccctttc
     2281 acgccttcgc aagaagcatc ggcacggaag agggctggga ctttgccgaa accgacgaag
     2341 cccgccgcaa cctcatcgca ggctttgccg aaatccacgc ccgaaaaggc acgccgtacg
     2401 ccatccgcgc cctcttcccc atcttgcggc tgggcgaaat ccaaattatc gaacgcgacg
     2461 gcgagttcaa gtgggacggc tcggtcttgt tcgacggcag ccgcacattc ggcaggcgca
     2521 agggtgactg ggcggaatac cgcattgtct taacgcgccc cgtcagcatc cgccaaaccg
     2581 cccgcatccg cgccatgttg gcggaaatcg cccccttgcg gtgcgaactt accgcgctcg
     2641 actaccgcaa ccatccccac cgctggaacg gcaaaatccg ctttaacggc gaatacggtt
     2701 tcggcacgac ataacgcgcc cccgaaaatc aacaaacaaa aggaagcccc aaaatggcaa
     2761 acgcaaccga acaaaaccaa ttcgaccaag ccgtccgcct catcgaatcc ggcgacagcg
     2821 tcgtcggccc aggcgcgccc gtcaaccaac cactgcaagc cctcgccaac cgcacccttt
     2881 tgctcaaaaa ccaaaccgaa gcccttcaga cggcatccga caccaaagcc gcaccatcgc
     2941 actgggcgcg cccgggcaaa tcaccgcaac aagccaaaac accgtcccaa aaaacggcca
     3001 cacccacgcc atcgacaccg cacgcaccga ccgcgccggc atcgtccgat tagacaatgc
     3061 cattagcgaa gccgaagaca ccgccgccac ccccaaagcc gtcaaaaccg cgctcgacca
     3121 agcccgcgcc gccgccgcga ccgccgacct caaagtctcc ctctccgaca accaaaccgt
     3181 taccggccaa aaaaccgtta ccggccaaaa aacctttacc gccgaaacac aattccaaag
     3241 cggcatccgg ctgtccgcca acccgacgca ctgaaacggc ggctacaaag cctatatcgg
     3301 cgcggataac gaaaacgccc acatcgtctt cggcgacgac accctgcgcc tgcacggcgc
     3361 aaataaccgc atttcctaca acaaccacga catcttccac aaagccaaca aaccgcgttt
     3421 caacgaagac atcgaaggca aaccgaacac gctgtccggc tacggcatcg gcaacttcaa
     3481 agtcgaaaca ttccggggcg atttgaacag cctcaaaacc gacggcgtct attccctgcc
     3541 gacggcggtc ggcagctcga acctgcccgt tgaaaacacc gcctgccata tccaagtcat
     3601 cgccggcacg caacccggct ggtgcaggca gttgggctat cccgcctaca cgtccgacgt
     3661 gtacgaacgc taccagacca gcagcgcaaa cgacgactgg agcgcgtgga aaaaactcaa
     3721 ttcggaaggc atccccgccg gcgcgatcgt gtcctttccc aaagccgtcc gaaaccccgc
     3781 aggctatctc aaagccgacg gcacgatctt tgcacaaaac accttccccg acctttaccg
     3841 cgccctgggc aacacaaaca aactgcccga cctgacccgt accgacatcg gcatcaccgc
     3901 gtggtttccg tccgaccaaa tccagacggg ctggctggcg tttgacgaca tccgaaagcg
     3961 cgtaaccgaa tcggcttatc ccgaacttta ccgcctgctg accgaaaaat acggacgcat
     4021 ccaaaacgtc ccgcaggcgg aagaccgctt tatccgcaac gcgggcaacg gcttggcggt
     4081 cggaacgaag caggaagacg aaatcaaacg gcacgtccac aaagtatttt cacactgggc
     4141 aaaccaccca gacgccgccg ccgtcggtta cgaagaccgc aatgaaaggc aaagaagcgc
     4201 gctcgtatcg acttggacgg acggcgactt aaacgacaac ggctttttaa ccccgcgatt
     4261 ggacagcaaa atggcaacgg gcggcgcaga aaaccgcccc aaagccctgg ttttaaaact
     4321 gtgcatcaaa gccgccgaca ccttgggcga agccgtgttc cggataaagt cccacggcga
     4381 aaccgccaac gtcggcgcac tggacgcgtc aaggctggcg caaggtttgc aggaaaaagc
     4441 cgaccgcgac cacacccaca ccaccgccca aatccaaggg ctggacgaaa aaatcagcgc
     4501 cgccgttgcc gcgcaattca cacgccaaac catcggcggc gtggatattg tcagattccc
     4561 cgacggcaca atgatacaga ccggcagtta caggtttgca agaggcggca gccccatagg
     4621 aaacgaagtt gtcttcccca tcgcctttgc cgacggcaac gtcaaatgct tcgtatccga
     4681 acgccattcg ggacgcgtca acggcgaaag gcaacacaac tggctgttta tccgcgcaaa
     4741 aaaccacgcc gccgccatta ttaccaactg gtacgaaagc agttgcgact ggatggccat
     4801 cggcaaatcc gcctcgggaa acgccgccag ccccaccccg atagtccccg aaatacctga
     4861 aatcgatgaa gaaccgcaaa gagagagtgg aagatcatca accggactcc gaaacccccg
     4921 ccgccaccga ggcctcgact tccccgtggg gtcgtaagac tagcgggcgg cagggcaggc
     4981 tgtagagacg ggcttcagcc caccgatcca agcaatccga ccgaaaccgg ccgcgccgcc
     5041 aatcctgcga aacctatgcc ccgccaatcc tgccactctt cgccattccc gcgaaagcgg
     5101 gaatccggaa cccaaacgcg gcaggaatct atcggaaaga ataacccgac cgccgtcatt
     5161 cccgcgcagg ctggaatcca gacccacaac gttaaggcgg tttatcagaa aaaaccgaaa
     5221 ccgaacgccc tagatttccg cctgcgcggg aatgacgaag agttggggag tgacgaaagg
     5281 cgggaacaac cgcgcaaaaa gccgccgact ccttcagacg gcatcggcaa taaaaaccgc
     5341 acggccgaaa ccgcgcggga aaggatagcc gggcgcgccc gataagcagc ggccgcaggg
     5401 ttaagtcctt gcacctgttt tcatcgaaga caaaggcggt tcgggaaata agggcgcaat
     5461 atcaaaagtt ttgcgttgcc tgatttgcca caaatcataa ccggcctgca tattcagcca
     5521 aaaatcaggc gaagtgttga gcagttggct taaacgaacc gccatctcgg gcgtaatcga
     5581 agattgggcg ttgacaatgc gcgataaggc aacccggctg acacccaggc gtttcgctat
     5641 atcggtaatg cttgcgccgt taatatactc ttttaaaaca aggcccgggt gggcaggatt
     5701 gtgcattttc atattattcc tagtgataat cctgataatc gacaacttcg acattgccgt
     5761 cgttgaaacg aaaagtcaaa cgccaatttc cgttgacttt gaaccgccca atgtcccgac
     5821 aaactgccct tgaggggatg aagattccaa ctcggcacag ccatatccga aggcgtttgc
     5881 gcggcattca gcgcggttag aagcagattc agtttgacgg aatgcccggc ttggatgccc
     5941 gatagcgagc ctgttttaaa aaagcgttca agccctttgt gtttgaaaga aacaatcatc
     6001 atcccacccc gtctgtatcg ccaaaggata caaaactttt atttgattgc aaagcgacga
     6061 tatacaaaaa aggaaacccc gaaatgacca tctatttcaa aaacggcttt tacgacgaca
     6121 cattgggcag cgtccccgaa ggcgcggttg ccgtcggcgg cgaagagtac gccgccctct
     6181 tcgcaggaca ggcgcagggc gggcaaattg ccgccgattc cgacggccgc cccgttttaa
     6241 ccccgccgcg cccgtccgag taccacgaat gggacggcaa aaaatggaaa atcagcaaag
     6301 ccgccgcccg tttcgccaac tcccgtttcg ccaaacaaaa aaccgccttg gcattcagcc
     6361 tcgcggaaaa ggcggacgaa ctcaaaacag cctcttggcg ggctatcccc aagtggaaat
     6421 cgacagcttt tacaggcagg aaaaagaaac cctcgcgtgg caggcggaca acgacgcccc
     6481 gaccccgatg ctggcgcaaa tcgccgccgc aaggggcata gaattggacg ttttgattgg
     6541 aaaggttgtc gaaaaacccg cccgcctggc cgttgccgtc gacgcgatta tcggaaagcg
     6601 gcagcaactt gaagatagat taaataccat cgaaaccgcg ccagcattag atgcgttaga
     6661 aagggaaatc aaagaatggg cgctaaacgt cggctgagag aatatattta tcatctacta
     6721 gtggccatag accaactgtt caacgctata accggcggcg cggcagacga aaccctctca
     6781 agtcgcacct atcgcggcgc gcggctcgcc caacaaccca aaacccgctg gaaga
//
LOCUS       DAXDZG010000092         6594 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXDZG010000092 DAXDZG010000000
VERSION     DAXDZG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.092"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..262)
                     /locus_tag="ACFB78_001873"
     CDS             complement(<1..262)
                     /locus_tag="ACFB78_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269807.1"
                     /translation="MPSLQRRSRGEKSSCIKQLNGGNYPPFLICCQLFFENLPDRFKF
                     CKKLARWLSGNKFPQKPVNPIIQKIICFHRVSFSVETLPFRAV"
     gene            complement(571..1035)
                     /locus_tag="ACFB78_001874"
     CDS             complement(571..1035)
                     /locus_tag="ACFB78_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC1269808.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1257..2315
                     /gene="alr"
                     /locus_tag="ACFB78_001875"
     CDS             1257..2315
                     /gene="alr"
                     /locus_tag="ACFB78_001875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC1269809.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2857..4212)
                     /locus_tag="ACFB78_001876"
     CDS             complement(2857..4212)
                     /locus_tag="ACFB78_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC1269810.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(4227..4499)
                     /locus_tag="ACFB78_001877"
     CDS             complement(4227..4499)
                     /locus_tag="ACFB78_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC1269811.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4624..5361)
                     /locus_tag="ACFB78_001878"
     CDS             complement(4624..5361)
                     /locus_tag="ACFB78_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC1269812.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB78_001879"
     CDS             complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB78_001879"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC1269813.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
BASE COUNT         1756 a         1640 c         1471 g         1727 t
ORIGIN      
        1 ctaccgccct aaagggcagg gtttcaaccg aaaaggaaac acgatgaaag catatgattt
       61 tttgaataat cggattgacg ggtttttgag ggaatttgtt accggatagc catcgggcaa
      121 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcagataa
      181 gaaacggcgg ataattaccg ccgttgagtt gcttgatgca gcttgatttt tctcctctac
      241 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcatttttta tctccgcttt
      301 ttttgaaatt taattacttt tattgtttga aattctattc tttaagaaat ttaacaagaa
      361 aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg gttcgagtag
      421 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctattgaatg
      481 tgtgcatttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg tttcatttag
      541 atgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg gattttcttt
      601 gacttcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt gcaataggat
      661 attgccgagc atatcccgat aggcagacat atcgggcaaa cgtactttaa tcaggtagtc
      721 gtattcgccc gacaccagat ggcactccat aatttgcgga attttcagca cttctttttt
      781 gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa aaaccaatgg
      841 cggtttgccc aacagatggg gattgagatg ggcgtgatag ccggaaatat aatgttcccg
      901 ctccaaacgg cataccctct ctgtaacggg cgtgctggac aaccctacct tctcggcaag
      961 ctccgtcatc gggatacggg cattttgctg aagaattttg aggatgcgga aatcgatttt
     1021 atctagttct ttcatttgga ttaccttgta tttattattg attttaacaa atagagtata
     1081 aatttgggaa agcaattata tcaggaaaag caaaccgcct tcctacctga aaactgctgc
     1141 tctggcttga agacacaagg ttctttaata ttttaaaagc cttgccgttg gattataatt
     1201 ccccaattga tttcttaatt ttgctaataa acacttgttt ggtaaggaat gaatttatgc
     1261 ggcctttgaa cgtgcagatc aggttgggca accttaggca caattatcgg attttgaagg
     1321 aaatgcacgg aggcaagctg ttggcggtag tgaaggccga cgcatacgga cacggtgcgg
     1381 tcagatgtgc tttcgcgctg gcagacttgg cagacggctt tgccgtggcg acaatcgacg
     1441 agggaatcag gctgcgggag agcggcatta cccatccgat tgtcctttta gaaggcgtat
     1501 ttgaagcatc agagtacgaa gcggtcgaac aatactcgct ttggccggca gtcggaaacc
     1561 aatggcagct tgaggctttg ctttcccgcc attggaaaaa acctgtcaag gtctggttga
     1621 aaatggattc gggaatgcac cgtaccggat ttttccctca tgattacgct tcggcatatg
     1681 cggcattgaa acagtcggaa tatgtggaca gtattgtcaa gttctcgcat ttctcctgtg
     1741 cggacgaacc tgaaagcggt atgacggaaa tacagatgga agcattcgat ttgggtacga
     1801 aagggctgga aggcgaagaa agccttgcca attcggcagc tattttgaat atccccgaag
     1861 cacgcaggga ttgggggcgc gcgggcttgg cgttatacgg catttccccg ttcggaggca
     1921 gcgatgacag gctgaaaccc gtgatgaggc tttcaacccg tattttcggc gaacgcgttt
     1981 tacagccgca ctcccctatc ggttatggcg caacatttta taccagtaaa tctacgcgcg
     2041 tcggtctgat tgcctgcggt tatgcggacg gttatccgcg ccgcgctcca agcaattccc
     2101 ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag gatctctatg gatatgatga
     2161 ctatcgagct ggacgcttcg caagaaggtt tgggacacga ggtcgaactg tggggcgata
     2221 cggtcaatat caataccgtt gccgaagcgg ccggaaccat cccttacgaa ttgatgtgca
     2281 atatcaaacg tgcaaaattc acttatatcg agtaatcgag tccaaacgaa aatcccgtct
     2341 gaagcttttc agacggcata aatgcgtttt gacaaaatca gtaaaagaca ttgccaaata
     2401 gaaaatactg ataatgccca caatctgaaa ccccatcctt cccgcaaaaa cggaaaatca
     2461 aaatcagaaa cctaaaattt cgtcattccc gcgcaggcgg gaatccggtt ttttgagttt
     2521 ccgtcatttc tgataaattc tcgtcgtttt tcatttctag attcccgcat gcgcgggaat
     2581 gacaaaatta aagtttcaga atttatttga acaacaaact agattcccgc tttcgtgggg
     2641 atgacggcgg ataggttttg ttgtttcgga taaattcctg ttgttttttg gtttccagat
     2701 tcccaccttt aaaagaatga cgtaagaaag cagaaataag aacacgggga ttttttaaat
     2761 ttgcaatatt cttttctaaa gacattttac ccaatgccca taaacaaaaa tgccgcctga
     2821 agcctttcag acggcatttt cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     2881 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcac gaaccttctt ttgccggcat
     2941 tacaggcgcg tagcccaaca gaccgccgaa ctcgacgctg tcgccgacgg ttttgcccgt
     3001 taccggaata atgcgcacgg cggtggtttt gctgttgatc atgccgatgg cggcttcgtc
     3061 ggcgatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatgtccaa
     3121 accaacggag cagacggcgg tcatggcttc gagtttgtcc agcgtcaaca cgcctgcctc
     3181 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccgc tcaaaccgcc
     3241 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     3301 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgcgccac
     3361 cgagtcgccg acggcggggg tcggtgccag cgacaaatcg agaatgccga acgggatatt
     3421 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtgattt tgaaagcggt
     3481 tttcttcacg acttcggcga cctcggtcag gctgaccgcg tccgaatttt ccagcgcggc
     3541 tttgaccacg cctggaccgg atacgccgac attaatcaca gcatccgctt cgcccgagcc
     3601 gtggaacgca cccgccataa acggattgtc ttccaccgcg ttgcagaaca cgacgatttt
     3661 ggcgcagccg aaaccttcgg gcgtgatttc agccgtgcgt ttgatggttt cgcctgccag
     3721 cttgaccgca tccatattga taccggcacg cgtgctgccg atattgatgg agctgcacac
     3781 gatatcggta gttttcatcg cttcgggaac ggaacggatc aacacctcat ccgaaggcga
     3841 catacctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     3901 tgccttgtcc aaagtctgcg ccacgctgac ataactgtcg gctttggtcg ccgccgcgat
     3961 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcgga
     4021 aaggtgtttc gccgttgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     4081 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     4141 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     4201 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttcg
     4261 ttttgcatac ggatgtcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaatc
     4321 tcctgccgcg atttagggca tttcgaggta tccaccaaga taatcatagt aaaaaaatcg
     4381 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     4441 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     4501 ggcttactcc ttgcagatat ccgttaaaat ccgaaattat accaccgtcg gattttgaag
     4561 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
     4621 gattcagggt tcgattaaat aaccatcctt atcccattgg gttttcctga ccaacttgtc
     4681 atcctgataa acagcctcgc tctttttaga accatcttca taccactcca aaaccacccc
     4741 gttgcgttta tggtggcgga tagacagttc cgaaagtaat cggccgcttt catcccaagt
     4801 cagaattttg gcaggctcat cgttgaccat aaccatttcc gtcttgatac ttccgtcggc
     4861 ataccattgc ttccatacgc cgttcgcctt attttgctta aactggattt cgctttcctt
     4921 gccgccgtta cggtaataac ggtatcccgt accctcactc aagccatttt tataaggcat
     4981 aaccgcagat tttttaccgt tcggatacca gttgacccat tccccgtccg gcttaccctt
     5041 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
     5101 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
     5161 tttcttcatc gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata
     5221 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
     5281 aacagaatag gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac
     5341 aatccgagat aatttcttca ttatgatagc gatataaaaa acaattaact tataaacaat
     5401 atggtaagga tttctcaacc aagcgtcaaa cccgaaacaa tgtatcgtaa aaatgccgtc
     5461 tgaaaacaaa tcgtcttcag acggcatttc cctccaactc actcttcgcc caataactgc
     5521 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     5581 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     5641 cctttaggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     5701 tccgccccgc tgcccaacgc caattccggt tcgtgcaaat attcctcagg caaagcccca
     5761 accgactctg catccacata aggcggattg gaaacaatca aatcataagt gccttccagt
     5821 ccctcaaaca aatcggtatg aataagctga atacgctctt ccagaccata atcttcaata
     5881 ttgatccctg caacttccaa agcatccaaa ctcacatcaa ccgcatcaat ttgggcatca
     5941 ggataatggt gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     6001 ttatgcacca gctcatcgta ttctatccaa ggtcgaagtc cgtcacccag caattcataa
     6061 ataaaagagc gcggtacaat cacgcgttca tctacataaa aatcaaactc cccctgccag
     6121 gcctggtgtg tcaaataagc ggcaggaatg tgttcgacag cacgacgctc aataaccgcc
     6181 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     6241 aaaggcaaat tcaaagtatg cagaatcaaa taaaccgctt catcatgcgc attatctgtt
     6301 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaacatca
     6361 cggatagtcg tcaattcttg tgcggcctga ttaaacataa tatgaaccat tctgcgtata
     6421 gatgctttta attataacag aaacaacaag caaacctttt catatcgcca aataaccact
     6481 caatctaccc atacaactac ataaatgccg cgcgaagacc attgcccgaa cggaaacggc
     6541 gatggcggac ggtataggca acctgatagg ctgggaagaa acggggcttg ttga
//
LOCUS       DAXDZG010000093         6436 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXDZG010000093 DAXDZG010000000
VERSION     DAXDZG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.093"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            43..708
                     /locus_tag="ACFB78_001880"
     CDS             43..708
                     /locus_tag="ACFB78_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC1269814.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQIANLLRFSRGEAVRLNLPVYICPAQVKKDGTVDNQCDFGKKEQGMLAFGDKNDNK
                     AYDGDAADVFLRNVVLNDDIKDKRINYTFNHIAFSSSQPTADRVVWTFNQNGTFGYTK
                     DQHLASTSSFFYSDGYIQIVLTDARAVSDADRKFRSAVVLIDSSGRVEVCRKNDTRAV
                     CKH"
     gene            740..1348
                     /gene="pilV"
                     /locus_tag="ACFB78_001881"
     CDS             740..1348
                     /gene="pilV"
                     /locus_tag="ACFB78_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC1269815.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMRNSIPSAVDGDFKVDAV
                     KSKVQLADEQLKRFGYELKNALPDAVAIHYAVCKDSSGKAPALSGDAFSSNCDNKENG
                     DTLIKVLWVNDSAGDSDISRTNLEVSGGNIVYTYQARVGGRE"
     gene            1345..2289
                     /locus_tag="ACFB78_001882"
     CDS             1345..2289
                     /locus_tag="ACFB78_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC1269816.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIEQDVVSDVTQKNSP
                     FSLKRNSTRNSTNKLIPIAESSNIGYQGFIQRLNALIFQYGIDDVNASAATTVVSSCG
                     AISKPSKQILTLEDAKKELEILNQDKEQNDNIARQRHVVNAYAVGKIAGEEGLFRFQL
                     DDKGKWGNPQLLAKKVKRMDVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEV
                     LLDSGSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            2268..2879
                     /locus_tag="ACFB78_001883"
     CDS             2268..2879
                     /locus_tag="ACFB78_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC1269817.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYTADSKVTFSENCEKGLCTAVNVRT
                     NNNGNEEVFGNIVVQGTPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYEKGTGNVSKMP
                     RYIIEYLGEKNNQNIYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            2881..3354
                     /locus_tag="ACFB78_001884"
     CDS             2881..3354
                     /locus_tag="ACFB78_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC1269818.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDDNDILKSKLEIFVSGYKMNPKIAKKYSVSVRFVDAEKPRA
                     YRLVGVPNAGTGYTLSVWMNSVGDGYKCRDATSAQVYLETLSANTGCEAFSNRKK"
     gene            complement(3424..3732)
                     /locus_tag="ACFB78_001885"
     CDS             complement(3424..3732)
                     /locus_tag="ACFB78_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFC1269819.1"
                     /translation="MKDLLSSPSFLLFSCMLAVTCPTRLIGFFALRNRTLSRRAQTVM
                     EAAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(3729..4436)
                     /locus_tag="ACFB78_001886"
                     /pseudo
     CDS             complement(3729..4436)
                     /locus_tag="ACFB78_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            4602..5054
                     /gene="dut"
                     /locus_tag="ACFB78_001887"
     CDS             4602..5054
                     /gene="dut"
                     /locus_tag="ACFB78_001887"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC1269820.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTLLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFAVKPFERIAQMVVVPVVQARFKRVEEFVGSSRGEGGFGSTGFH"
     gene            5130..6317
                     /gene="dapC"
                     /locus_tag="ACFB78_001888"
     CDS             5130..6317
                     /gene="dapC"
                     /locus_tag="ACFB78_001888"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC1269821.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
BASE COUNT         1728 a         1389 c         1700 g         1619 t
ORIGIN      
        1 aatttaatcc actatacatt accaattaac gtcctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggcca ttgcagccat tatggcgacg
      121 atagccctcc ccaatatgag tgggtggatt gcatcacgcc gcattgccag tcacgcggag
      181 cagattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgcccaagt taaaaaagac ggtacggtcg acaaccaatg cgacttcggt
      301 aagaaggaac aggggatgtt ggctttcggc gacaaaaacg acaataaggc atatgacggc
      361 gatgctgcgg atgtttttct ccgtaacgtg gtattgaatg atgatatcaa ggataagcgg
      421 attaattaca ccttcaatca tatcgctttc agttcgtctc agccgaccgc cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttgcaagc
      541 acatccagct ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataggaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gtcgaagttt gtcgtaaaaa cgatacgcgc gccgtatgca aacattaatg actgttttag
      721 tcggtttggg aaataaaaaa tgaagaataa tgattgcttg cgcctgaaaa atccccagtc
      781 cggtatggcg ttgatagaag tcttggtcgc tatgctcgtt ctgaccatcg gtattttggc
      841 attgctgtcc gtacagttgc ggacagtcgc ttccgtcagg gaggcggaaa cgcaaaccat
      901 cgtcagccaa atcacgcaaa acctgatgga aggaatgttg atgaatccga ccattgattc
      961 ggacagcaac aagaaaaact ataatcttta catgagaaac tctataccat cagctgtgga
     1021 tggcgatttt aaggttgatg ccgtgaaaag taaggtgcag ttggcggatg aacaattgaa
     1081 gagatttggt tatgagctga aaaatgcctt gccggatgcg gtagctattc attacgccgt
     1141 ctgcaaggat tcgtcgggta aggcgccggc attgtccggc gatgcttttt cttcaaattg
     1201 cgacaataag gaaaacgggg atactttaat taaagtattg tgggtaaatg attcggcagg
     1261 ggattcggat atttcccgta cgaatcttga ggtgagcggc ggcaatatcg tatataccta
     1321 tcaggcaagg gtcggaggtc gtgaatgaaa cgtaaaatgc taaacgtacc aaaaggcggt
     1381 tatgatggta tgaagggttt taccattgtt gaatttctgg ttgcgggcct gctcagtgtg
     1441 attgtcctga tagcggtcgt atcgagttac tttacatccc ggaaattaaa tgatgcggca
     1501 aacgagcgtc ttgccattca acaggatttg cggaatgcgg caacattgat tgtccgcgat
     1561 gcaagaatgg cggggagctt cggttgtttc aatatgtccg agcatatcga acaggatgtt
     1621 gtttccgatg tgacgcaaaa aaactctcct ttttccttaa aaaggaacag tacaaggaac
     1681 agtacaaata aacttatccc catagcggaa tcttcaaata tcggatatca gggttttatc
     1741 cagcgcctta acgcattgat tttccaatac ggaatcgatg atgttaatgc aagcgccgcg
     1801 actaccgtcg tcagcagctg tggcgcaata tcgaaaccga gtaagcaaat ccttacttta
     1861 gaagatgcaa aaaaagaatt ggagattctg aatcaggata aggagcaaaa tgacaatata
     1921 gcgcgtcaaa ggcatgtggt caatgcctat gcggtcggca agattgccgg tgaggaaggt
     1981 ttgttccgct tccaattgga tgataagggc aagtggggta atcctcagtt gctcgcgaaa
     2041 aaggttaaac gtatggatgt gcggtatatc tatgtttccg gttgtcctga agatgaagat
     2101 gccggcaaag aggaacaatt caaatatacg gataaattcg acagctctgt tacgcctgcc
     2161 ggggtggagg ttttattgga tagcggtagt gatgccaaga ttgccgcttc ttcagataat
     2221 attatttatg cttaccgtat caatgcgaca atacgcgggg gaaatgtatg cgcaaacaga
     2281 acactttgac aggaatcccg acttctgacg gacagagggg gtccgcactg tttatcgtgc
     2341 tgatggtgat gatagtcgtg gcctttttgg ttgtaactgc cgcccagtcc tacaataccg
     2401 aacagaggat cagtgccaac gaatcagaca ggaaattggc tttgtcttta gccgaggcgg
     2461 ctttgaggga aggcgaattt caggttttgg atttggaata tactgcggat agtaaggtta
     2521 catttagcga aaactgtgaa aaaggcctgt gtaccgcagt gaatgtgcgg acaaataata
     2581 atggtaatga agaggttttt ggcaatatcg tggtgcaagg cacgcccacc gttgaggcgg
     2641 tgaagcgttc ttgccctgca aagtctggca aaaattctac cggcctgtgc attgacaatc
     2701 agggagtgga atatgagaaa ggtacgggaa acgtcagcaa aatgccgcgt tatattatcg
     2761 aatatttggg cgagaagaat aaccaaaata tttacagggt tactgccaag gcttggggta
     2821 agaatgccaa taccgtggtc gtcctgcaat cttatgtagg caataatgat gagcaataaa
     2881 atggaacaaa aagggtttac attgattgag atgatgatag ttgtcgcgat actcggcatc
     2941 atcagcgtca ttgccatacc ttcttatcag agttatattg aaaaaggcta tcagtcccag
     3001 ctttatacgg agatggtcgg tatcaacaat gttctcaaac agtttatttt gaaaaatccc
     3061 caggacgata atgatatcct caagagcaaa ctggaaatat ttgtctcagg ctataagatg
     3121 aatccgaaaa ttgccaaaaa atatagtgtt tcggtaaggt ttgtcgatgc ggaaaaacca
     3181 agggcataca ggttggtcgg tgttccgaac gcggggacgg gttatacttt gtcggtatgg
     3241 atgaacagcg tgggcgacgg atacaaatgc cgtgatgcca cttctgccca ggtctatttg
     3301 gagaccttgt ccgcaaatac cggctgtgaa gctttctcta atcgtaaaaa atagggttgt
     3361 tttgccaata ccgtctgaag atcaattttc agacggtatt ttttatgggc attttgaaat
     3421 gaattatgcc atcaaccatc ccgagatgcc tgacgagccg acccctatca atacggtaaa
     3481 caacatagaa aaacggcagg ccgcaaaggc ggttaacgcg atggcaataa gttcgtgcgg
     3541 cttgtcggag acaaaatagg gggcaatgac ggaaatgagg acgcagcccg gcgcggcttc
     3601 cataacggtt tgggcgcggc ggcttaaggt tcggttacgc agtgcgaaaa agccgattaa
     3661 ccgggtaggg caggtaacgg caagcataca ggaaaacagc aggaatgatg gggacgacaa
     3721 taaatctttc atttccgccc attccaaaaa taggcggaca acaggccgga cagcgtgccg
     3781 gcgggaacat accacgcgcc gtcaacggtc aggtaaacgg cggatgcgac aaacaggttg
     3841 acaaatcaag gtattgatgc ggaaagattt ttccacattc ccctcagtaa aaccagaaat
     3901 acggcaggaa acgccatacc gaacccccaa gcggccacat tgccgaaccc agcctactgc
     3961 cgcgccgagg gcggcaaacc ctatcaggta atgtagagga taaagcatgc gcccatataa
     4021 gaaggcatat tgaatgcagg caaaccggct gctttccgtt tttggatttc ggagaatgcc
     4081 atggcccggc tttcatcaca cataaaaaac agcgcgggcg cggctttttt cagcggtatt
     4141 cctttcaggt gcgtggcaag cgccgccccc atcaggatat gccgcgaatt aatcataaag
     4201 gttatggtgg cgataagcag tatcggcaga ggttccgccc acaggttgac cgtggcaaat
     4261 tcggagccgc cggcaaagtt catccccgtc atcagcagca tttccagccg ggccatacct
     4321 ttttgcccgc cctgcattcc gagtattaat gcccaaggca gcagcccgat aagcataggc
     4381 gagctttctt ttatgccgcg tatgaattca tcacgtggag aggctgtgtg cgtcatgtta
     4441 atgtttatat atgaagagac ggggcggtat catacacagg cagaatgttt ttgacagtcg
     4501 aggatggttt caaaacaaaa gcagtttgtt gtgtttcagc gtaaacacgg cttgtgtata
     4561 atctccaatc tttgaaaccg gccgtatgca ggagcaggac gatgaatatt gaagtagaaa
     4621 tgaaagtgtt ggacgagagg atggcggatt ttatccctgc ctatgcgacg gagggttctg
     4681 caggtttgga tttgcgcgcc tgtttggatg aggaagtcgt tttgcagccg ggcggaacgt
     4741 tgcttgtgcc gacaggtttg gcaatttatt tggcgaatcc cgcatatgcc gctgttttgc
     4801 tgccacgttc cggcttgggg cataaacacg gcattgtctt gggcaatttg gtcggtttga
     4861 ttgactccga ttaccaaggg gaattgaagg tgtcgttatg gaataggggt agcgaaccgt
     4921 ttgccgtcaa gccgttcgag cgtatcgcac aaatggttgt cgtgccggtt gtgcaggcgc
     4981 gtttcaaacg tgtcgaggaa tttgtcggaa gcagccgggg tgagggcggc ttcggcagta
     5041 cgggtttcca ttgaatatag aatgccgtct gaagggcacg tcagcttcag acggcatatc
     5101 ttccgatata ccgataaccg gagaaaatca tgaatacctt actcaagcag ctcaaaccat
     5161 atccctttgc ccgactgcgc gaggcgatgc agggcatttc cgcgcctgaa ggcatggaag
     5221 ccgtcccact gcatatcggc gaaccgaaac acccgacacc gaaagtcatt acggatgcgc
     5281 tgaccgcctc attgcgcgag ttggaaaaat atccgctgac ggccggtttg cctgaattgc
     5341 gtcaggcgtg tgcaaactgg ttaaaacgcc gttacgatgg tttgacagtg gatgcggata
     5401 atgaaatttt gccggtttta ggcagcaggg aagccctgtt ttcttttgtt caaaccgtgt
     5461 tgaaccctgt ttcagacggc ctaaaacccg taatcgtcag cccgaatccc ttttatcaga
     5521 tttacgaagg tgcgacactt ttgggcggcg gtgaaatcca ttttgccaat tgccccgcgc
     5581 cgtctttcaa ccccgattgg cgcagtattt ccgaagaggt ttgggaacgc accaaactgg
     5641 tgttcgtctg ctcgcccaac aaccccagcg gcagcgtgcc ggatttggac ggatggagag
     5701 aagtttttga tttacaggat aaatatggtt tcattattgc ctcggatgaa tgctattccg
     5761 aaatctattt cgacggcaac aaacctttgg gctgcctgca ggccgccgca cagtcggggc
     5821 gaagcaggca aaaactgctt atgttcacca gtttgtccaa gcggtccaat gttccgggtc
     5881 tgcgttccgg ttttgtcgcc ggcgatgcgg aactgcttaa gaactttctg ctttaccgaa
     5941 cctatcacgg cagcgcaatg agtattcccg tgcagcgtgc cagtattgcc gcttggaatg
     6001 acgaacaaca cgtaatcgac aaccgccgcc tgtatcagga aaaatttgag cgtgttattc
     6061 ccattttgca acaggtattt gacgttaaat taccggatgc ctcgttttac atctggttga
     6121 aagtccccga tggagacgat ttggcatttg cacgaaattt atggcaaaaa gcggctatcc
     6181 aagtattgcc cggacgcttt ttggcgcggg ataccgagca ggggaatccc ggggaaggtt
     6241 atgtccgtat cgctttggtt gccgatgtcg caacttgtgt caaagctgcg gaaaccattg
     6301 tttccctata tcggtaaaga ataaaaaata ccgtctgaac ttttgttcag acggcatttt
     6361 tcaatatttt acggttgaat ttgctataac ggtatttata gtggattaac aaaaatcagg
     6421 acaaggcggc gagccg
//
LOCUS       DAXDZG010000094         6249 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXDZG010000094 DAXDZG010000000
VERSION     DAXDZG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.094"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(33..723)
                     /locus_tag="ACFB78_001889"
                     /pseudo
     CDS             complement(33..723)
                     /locus_tag="ACFB78_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1425..2267)
                     /locus_tag="ACFB78_001890"
     CDS             complement(1425..2267)
                     /locus_tag="ACFB78_001890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1269822.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2502..3422
                     /gene="pyrB"
                     /locus_tag="ACFB78_001891"
     CDS             2502..3422
                     /gene="pyrB"
                     /locus_tag="ACFB78_001891"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC1269823.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSCVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3432..3890
                     /gene="pyrI"
                     /locus_tag="ACFB78_001892"
     CDS             3432..3890
                     /gene="pyrI"
                     /locus_tag="ACFB78_001892"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC1269824.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            3953..4618
                     /locus_tag="ACFB78_001893"
                     /pseudo
     CDS             3953..4618
                     /locus_tag="ACFB78_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5023..6246)
                     /locus_tag="ACFB78_001894"
     CDS             complement(5023..6246)
                     /locus_tag="ACFB78_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC1269825.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     DISGKYLYSPWQWGSLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KMSPDKEVSGYGIPAVNANFYRIFIRHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
BASE COUNT         1490 a         1520 c         1751 g         1488 t
ORIGIN      
        1 ctatataaaa taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt
       61 tctaccgtaa aacgtgtttg acgaacggga tttttattaa aaaacatctg atttctaaca
      121 aaatcagtat tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt
      181 ttccatgcgc tcaagaattg tgatttgctc gttgagacgt gccccagcga tggatcagcc
      241 agcaaaacag tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat
      301 tttaaataca caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa
      361 ccttgtgctt caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc
      421 atttgggttt tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa
      481 ttattcagta acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact
      541 atgccggaat ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg
      601 gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca aagccgcata
      661 atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa acgcatgaaa
      721 cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat aaaatagata
      781 atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata ataaaataat
      841 ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt aatacaccca
      901 taatcatgca acctatatta ataaatagct aactaattct attacagaaa ttagttagct
      961 gattaaaagt gattaacaat tatagtggat taacaaaaat caggacaagg cgacgaagcc
     1021 gcagacagta caaatagtac gaaaccgatt cacttggtgc ttcagcacct tagggaatcg
     1081 ttctctttga gctaaggcgg ggcaacgccg tactggtttt tgttaatcca ctataaatag
     1141 cgagcatcga ttcacggtgc gctttagtgc aaagcgtaca acgcgagcct gaaccaccag
     1201 ccgcaatagg aaaagaaagc cgatagagtg caatgcttgc caacgcgcaa gcgagcttgc
     1261 aagaacgctt ggctcaacga gagcaggcaa gacagaaagc aggataggag aggcaacgca
     1321 aaggtctcgg gctttgattt cgccgtaaac ctgagtgccg ccttgtccgg acggggtggt
     1381 tcaggcggcg ggtgccgaca gggtgcagat gaggaggagt attttcattt ggggtcgcaa
     1441 cggaagtggt aggcgcagat ttcaaaaccg tttttgaaat acaggcggtg cgcgtcggta
     1501 cggtcgtggt tgacgtggac gttaaggtgg attttggtaa cccctgtttc cgcgctgatt
     1561 ttgcggactt cttccaaaag gcgcgaggcg tagcctttgc ggcggctttg cggcagggta
     1621 acgatgtcgt cgatgtggat gtggcggccg ccggcgaggg tgcaggcttc gcggaagccg
     1681 cagacagcga cggcgttgtg tttgccttct tcaaaaatgc ccagcaggcg gtagccttgg
     1741 gggcgttggg ttttgttgat ctgttcggta aagcggccga tgtcggtcag ggcggaacgc
     1801 aaaacgctca aggctgcaaa ggcggtggcg gtgtcgtccg cgccgatttc gcgtaaaacg
     1861 taggatgcgc ccgaggcggt ctgttcctgt gttttctcgg cggcgtgttt ttcttcgatt
     1921 gcctgtgcca gcaggacgcg ttcgttggca gggtcgtttt gtccgccctg ttcgcgttct
     1981 tcgagcaggg ctttgcagtc gatgacgcgc aggtcgttgt cggcggcaaa gtccatcagg
     2041 aagcggaaca tctgcggatt gtcggtttcc agttttttat cgacggcgac gcagcggatg
     2101 ttgtccacca gcatggggcg cacccatttc gaataggaga ggcggttgtc tttggtgaac
     2161 gaggacagga tgccgcacag gcgttccgcc cagtcgccgg ggcggaaaat cttgccggaa
     2221 ctcgttgtgc cgtggatgac gacttcgtag gggttgcaga ttaacatggc ggcttcctga
     2281 aaagaaatgt ctggcgcgat tataccttat gcttatgcgg gcgtgtttgg atatgccgtc
     2341 tgaaaaatac aggattcgtg cggtaaaact ttgtggaggc aaatgtgcga taatacgcgc
     2401 cgtgtcgccg cttttgcgaa gctgttccgc aaacatacgg gcggcgtgga cgtataaccg
     2461 gatgcccgcc tgacgcgggt tttttacgga aggggggcaa aatgcctaat ccgctttaca
     2521 gacagcacat catctccatt tcggatttgt cgcgcgaaca gttggaatgc ctgcttcaga
     2581 cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt ggaaggcaaa ctcatcggtt
     2641 cgtgcttttt cgagccgtcc acgcgcacga ggctgtcgtt tgaaacggcg gtgcagcgtt
     2701 tgggcggtaa ggtcatcggc ttttcggacg gcgcgaatac cagtgccaaa aaaggcgaaa
     2761 cgcttgccga taccgcccgt atcatttccg gctacaccga cgcgattgtc caacgccatc
     2821 ccaaagacgg cgcggcgcgc gtggcggcgg agttttcgtg cgtccccgtc atcaacgccg
     2881 gcgacggcac gaaccagcac cctagccaaa ccttgttgga cctggtaacg atttatgaaa
     2941 cgcaggggcg tttggacaag cttaaaatcg ccatggcggg cgacttgaaa tacgggcgca
     3001 ccgtgcattc gctgtgtcag gcgttgaaac gctggggctg cgaatttgcc ttcgtctcgc
     3061 cgcccagcct cgcgatgccc gactacatta ccgaagagtt ggaagaagcc gactgccgat
     3121 accgcgccct cggcagtttg gaagaggcgg cggaatgggc ggacatcttg tatatgaccc
     3181 gcgtccagcg cgaacgtttc gacgaacagg aatttgccaa aatccaaggc aaattcaacc
     3241 tcgaagcgtc tatgctcgcc cgcgccaaac cgaacctgcg cgtgctgcac cccctgccgc
     3301 gcacggacga aatccacccc gatgtcgatg ccgcgccgca cgcctattat ttcgagcagg
     3361 cgaccaacgg cgtttatgcg cgtatggcga tattgtcgct ggtgttgaac gaagaagtgt
     3421 gaggaaccga tatggaagcg caaaaactca gtgtcgaagc catcgaaaaa ggcacggtta
     3481 tcgaccatat tcccgccggc agggggctga ccatcctgcg ccagttcaaa cttttgcact
     3541 acggcaacgc ggtaaccgtg ggcttcaacc tgcccagcaa aacccagggc agcaaagaca
     3601 tcatcaaaat caaaggcgtg tgcttggacg ataaagccgc cgaccgcctc gccctgttcg
     3661 cccccgaagc ggtggtcaac accatcgaca atttcaaggt cgtacagaag cggcatttga
     3721 ccctgcccga cgaaatcgcc gaagtgttcc gctgcccgaa tcccaattgc gccggccacg
     3781 gcgagccggt caaaagccgg ttttatgtta agaaacacaa cgggcagacg cggctgaaat
     3841 gccactactg cgaaaaaacc tacaaccggg attcggtggc ggaagcctga cggattcccg
     3901 cagcccggcg gcggaatttt gcctgccgcc tgttttgcca atctgaaatg gaatgatgat
     3961 gcacgcttct gtccaaagtc gtttcgcacc gatactttat gttttgattt tctttgccgg
     4021 ttttttgacc gcgcaaatct ggttcaatca gaaagcctat actgaagagc tgcctccgct
     4081 tctgtccgca ttgtccgccg tcgcgctggt gtggctggcg tgggcgttcg tgtcggtgcg
     4141 ttcaaaggct aaggcagaaa agttctaccg cgaaaaaatg atacagaacg aaagcatacc
     4201 cccgtcctgc acgcttcttt gcaacacttg gaacacaagc cgcaaatgct cgccctgctg
     4261 gtcaaaaacc acggcaaagg catggcggaa caggtcaggt tcaaggcgga agtgctgccc
     4321 gacgacgaag acgcgcgcac gattgccgcc gagttggcaa aaatggatat gttcgcattg
     4381 gggacggacg cggtcgcctc gggcgaaacc tatgggcgcg tgttcgccga tattttcgag
     4441 ttgtcggcgg ctttggaagg gcgcgcgttc aaagggatac tgaaactgac ggcggaatat
     4501 aaaaaacatc ttcggcgatg cctgccgttc ggaaacggcg ttggatttgg gcgcgctcaa
     4561 tcaggcgttg agggaaatct cgaaaacgcc ggaaaagcct aagcagatat tttattgaag
     4621 gtgtcccgat gtatggggag cgcacgggtg gcgcggcggc aaagcggcaa gattgggttt
     4681 ggatacccgg ttggttttat agtagattaa caaaaatcag gacaaggcgg cgggccgcag
     4741 acagtacaaa tagtacggaa ccgattcgcc cggtgcttgg acaccttagg gaatcgttcc
     4801 ctttgagccg gggcggggca acgccgtacc ggtttttgtt aattcgctat atctgtctgc
     4861 aagggagaca gccgctgtga tttccgttcg ggcggaaacc gattccatcg gataaaaggc
     4921 attttgtccg actaaatcag ggttttgcat caacgggata tgaaaacaca gcctaaataa
     4981 aaatgccgtc tgaatgctcc agacggcatt gttgccgagg ggttattgga aagggtcgga
     5041 atcgacgcgg gcgcggccct gatccggcat attgtccaaa tcccgtcccg gattggcggc
     5101 ggtgtcgcct tcggaaatat cggagatgtt ttccaaaata atggcgtaag ccagtttcgg
     5161 cgcagtgtgg taaaccattg ccaagcccac ttcttcggca ggtgtcgaaa tcagctcgac
     5221 ggtatccctg cttttaggtt cttcggtcaa attgttggag aggttgacct gcatcgtttt
     5281 cttgcgtttg taaaggctta aaactgcgcc tttgtccaaa ccgtcgtccc cgcctttgtc
     5341 tatcgtaatg gttttgaact gtccgccgac ccccacgcct tcaaacacgg aaacgatttt
     5401 tgcctgaacc gggcgggacg gttcgtgcgg caccatattg aagcggtcgg tgtcttccgg
     5461 cattttcatc aggtagtcgc cctgctgtat ttcggaaatg gcggtttcga ccaccagcgg
     5521 ctgtatcgaa cgggtgcgca ccggggtaat caaaggatgg gtgcgggtgt agtattcgtt
     5581 gtctttcagc cgttcttcag cctgtttcga gcgttgttcg agggcggagt cggtatagtc
     5641 gagggagcgc acgatgccgc tgaatgcgac ttcttgtccg aggaatttac ccgtatccgg
     5701 atcggtgatg tttttattga tgcggtaggt caggtagcgg cccggctctt tcaggccttt
     5761 ggtgtaaacc ctggcgcctt tggtgtacag cagcctgcct tccgggcccg agagcaggcg
     5821 cggcgcggca gcggtttctt tgcgggaaac gatttgcggg tgccgtataa agatgcggta
     5881 gaagttggca ttgacggcag ggatgccgta tccggacact tctttatccg gactcatttt
     5941 gacgacgggg atgccgtctg tctgttccaa atcaaggcgc ggttcgccgc caacgtagcg
     6001 caacaccaat acctggcccg gataaatcag gtcgggattg tggatttgat cccggttcgc
     6061 gccccacagg ctgccccatt gccacgggct gtacaggtat ttgccggaga tgtcccacag
     6121 ggtgtcgccc tgtttgaccg tgtagcgttg cggcgcatcc gggcgcacct ccagatttgc
     6181 cgccaaagtt tgtgttgaga atgccatgcc tgccgcgcag agcagggtta taatacgacg
     6241 ttgcataac
//
LOCUS       DAXDZG010000095         5930 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXDZG010000095 DAXDZG010000000
VERSION     DAXDZG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.095"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..338)
                     /locus_tag="ACFB78_001895"
     CDS             complement(<1..338)
                     /locus_tag="ACFB78_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269826.1"
                     /translation="MGGIGGMTKYKFPCGQIRIPACAGMTDGNGARTFAGIFYVSILI
                     LFLMRYSKKIGCAGCFYFSDDLKSTAVNPICFSNRLQPQNLLQPAPSPVGEGRGEGIF
                     QVAATFPNTLT"
     gene            complement(547..702)
                     /locus_tag="ACFB78_001896"
     CDS             complement(547..702)
                     /locus_tag="ACFB78_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269827.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(719..2515)
                     /locus_tag="ACFB78_001897"
     CDS             complement(719..2515)
                     /locus_tag="ACFB78_001897"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC1269828.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTANEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2974..4227
                     /locus_tag="ACFB78_001898"
     CDS             2974..4227
                     /locus_tag="ACFB78_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC1269829.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAAPSLMFDAGTVAALIMASCLIIVVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKPGNAELFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4382..5893
                     /gene="lysS"
                     /locus_tag="ACFB78_001899"
     CDS             4382..5893
                     /gene="lysS"
                     /locus_tag="ACFB78_001899"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC1269830.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDGFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1261 a         1562 c         1722 g         1385 t
ORIGIN      
        1 gttagggtgt tgggaaaggt tgccgcaact tggaaaatgc cctctccccg gccctccccc
       61 acgggggagg gggcaggttg cagcagattt tgcggttgca ggcggtttga aaaacagatt
      121 ggatttacag ccgttgattt caggtcgtct gaaaagtaaa aacagcctgc acaacctatt
      181 ttcttggaat agcgcattaa aaagagtatg agaatggaga cataaaaaat tccggcgaat
      241 gtccgtgcgc cgttcccgtc cgtcattccc gcacaggcgg gaatccggat ttgtccgcac
      301 ggaaacttat atttcgtcat tccaccaatc ccgcccacag cccacttttc cgcgtcattc
      361 ccgcgcaggc gggaatccag tccgttcggt ttcggttatt tccgataaat tcctgctgct
      421 tttcattcct agattcccac tttcgcggga atgacgggat ttcagggtaa agatggcggc
      481 agccggaggg ctgtgaagag ctgttttcgc aggaatgacg atatgcggat ttgctgtttt
      541 catccgctac cgatgccgtc tgaagcctgt ttggcgtttc agacggcatt gggttttttg
      601 tgcatttgcc gcaaaacgga tcggtgtctg cttgcggcaa gacgataacg gtgcggaatg
      661 ttttgggaaa ccaatgccgt ctgaagggct ttcagacggc atttttgtac cgtgccgttt
      721 aacgcgccag cggttcggtg cgtttgatca gccacgcttt tgccgcgcct tcggtcagcg
      781 gctcgaggcg gcggcggact tcggcgtggt agcggttgac ccagtcgatt tcgccgtcgg
      841 tcatcagggc ggtgtccatc aggcgtgtat cgatggggca gagggtcagg gtttcaaaac
      901 agaggaagct gccgaactcg gtttcctgcg ggtcggcgac ggcttggttg gcggcgaggt
      961 tttcaatgcg gatgccccat ttccccggac ggtagaggcc gggttcgatg gaggtaacca
     1021 tgcctttttt catggcggtt tcgggcgtgg cgggggcggc gaaggcgatg cgctgcgggc
     1081 cttcgtggac gttgaggaaa tagcctacgc cgtggccggt gccgtgtccg tagtcgcatt
     1141 gcgcctgcca caggggtttg cggcaaatcg cgtcaatcag cggcgagggg atgttttcgg
     1201 ggaacacggc ttcggcaagc gcgatatggg ctttgagaac gagcgtgttg tcgcgttttt
     1261 gttctgctgt gggcgtgccg acggggacga cgcgggtgat gtcggtcgtg ccgcctttgt
     1321 attgcgcgcc ggagtcgatg agcagcagac cgttgccgct gatggtgctg tggctttcgg
     1381 gtgttgcgct gtaatgcggc agggcaccgt tggcgttgaa gcctgcgatg gtgtcgaaac
     1441 tcaaggacac aaaacccggg cgcgcgctgc ggtggcggta aagcatcgta tccacgtcga
     1501 tttcggtcag gctgccgcct ttgccgatga tgtcttcaaa ctcggcgaag aagccgcaca
     1561 acgccgcgcc gtcttgttcc atcgcttcgc ggatgcgggc gatgtcggct tcggatttga
     1621 cggatttgaa gaaggtggac gggttgatgc cttcaatcag gcgcgcgctt tcgggcaggc
     1681 gcacgagcgt actgacggcg gttttgttcg gctcgatgag cagcgcgccg ccgatttgcg
     1741 cgagtttgtc ggcaacttgg gcgtaaggct cgaccgtgat acctgcggtt tgcagcgcgg
     1801 cggcggcttc ggcgttcaga cggcattgtt cggtaaacag gacggcactg tctttgccga
     1861 tcagcaggaa ggacacgaaa acagggttga aaggcacgtc gctgccgcgc aggttggtca
     1921 gccaagcgat gtcgtcaagc gaggaaacca agtggtaatc cgcgcctttt tccgccataa
     1981 ctgcgcgcac gcgggcgagt ttctcggcgg cggtttcgga gacgtaggcg tggtcgtgga
     2041 tgaacacggt ttcggcgggg atagcgggac ggcttgtcca cacttggtca agcaggtcgt
     2101 cggggtgttg gatgcggatg tttttggcgg cgagcgattg cgccaaagtg cgtttgccgg
     2161 tgagcgacac catatcggaa gggatgccga cggcagcgtt ttcgggcagg tttgccgcga
     2221 gccattcgtt gtacggcggc acttgcccgc ttttttgcag ctcaatgccg ctgccggaaa
     2281 gctgtttggc ggcttgttcc caatagcggc tgtccaccca tacgcccgct tcattggcgg
     2341 taacgacgaa tgtgccgacc gagccggtaa agcctgacaa ttcgcgccgc gcctgccaat
     2401 gctcgggcag gtattcggat aggtgggggt cggcggaagg gatgacgagt gcgtccaagc
     2461 cttgcgcctt catggcttcg cgcaatgcgg acaggtaatt cgatacggta ttcataacag
     2521 ttcctccaat cgggtttcgc ggcttcagac ggcatggacg gaaatctgaa atgccgtctg
     2581 aaaaacataa ccatattatc aaatcgccag cctgctgcaa aacggacggg ctgatcggat
     2641 ggtgcgtgtt tgcacgaaaa aaagcgatgt aaggttgttt gttgtaaaat ttcaggcata
     2701 aaatgtcgtt tcatgccttc ttttggaagt aatacggaaa atatagtcag gtttcagcat
     2761 gaagttctaa tagtgtgtgt tggcaggtat ttggattacg ttacaatacg cgctttcatc
     2821 aaaatttccc taccgggcgg tatcctgttt ttgaagacgg taggatttgg gtttgtttgg
     2881 ggtcaatccc gcccaaattg gagcaataga tagggctgtg tccgaatatg cggctctgta
     2941 tgttgaaaca cagttaaaaa tacggaagac tttatgtccg aacaacatat ttcgacttgg
     3001 aaaagcaaga tcaacgcatt gggaccgggg attatgatgg cttcggcggc tgtcggcggt
     3061 tcgcacctga ttgcctcgac gcaggcgggc gcgctttacg gctggcagct cgcgctcatc
     3121 atcatcctga ccaacctctt caaatacccg tttttccgct tcagcgcgca ttacactctg
     3181 gacacgggca agagtctgat tgaaggttat gccgagaaaa gctgcgttta tttgtgggtt
     3241 tttttgattt tgtgcatcat ttccgccacc attaacgcgg gcgcggtcgc cattgtaacc
     3301 gccgccatcg tcaaaatggc ggcaccctcg ctgatgtttg atgccggcac ggttgccgcc
     3361 ttgattatgg catcctgcct gattattgtg gtaagcggac gctatcgcgc tttggatcgt
     3421 gtttccaaaa tcatcattgt tactttgagc atcgccacgc ttgccgccgc cggcatcgct
     3481 atgtcgcgcg gtatgcagat gcagcccgat tttatcgagc cgacaccgtg gacgcttgcc
     3541 ggtttgggct tcctgatcgc gctgatgggc tggatgcccg cgccgattga gatttccgcc
     3601 atcaattctt tgtgggtaac cgaaaaacaa cgcatccggc cttccggcta ccgcgacggc
     3661 attttcgatt tcaacgtcgg ttatatcgcc agtgcggttt tggctttggt tttccttgca
     3721 ctgggcgcgt ttgtgcaata cggcaacggc gaagcagtgc agatggcggg cggcaaatat
     3781 atcgggcaat tgattaatat gtatgccgta accatcggcg gctggtcgcg cccgctggtg
     3841 gcgtttatcg cgtttgcctg tatgtacggc acgacgatta ccgttgtgga cggctacgcc
     3901 cgcgccattg ccgagcccgt gcgcctgctg cgcggcaggg aaaaacccgg caacgccgaa
     3961 ctctttgcct ggaatatttg ggtggcgggc agcggtttgg cggtgatttt ctggttcgac
     4021 ggcgtaatgg cgaatctgct caaatttgcg atgattgccg cctttgtgtc cgcccctgtg
     4081 ttcgcctggc tcaactaccg cctcgtcaaa ggggacaaac gccacaggct taccgccggt
     4141 atgaacgccc ttgccattgt cggcctgctc tacctggccg ggtttgccgt tttgttcctg
     4201 ctgaacctga cggggctttt ggcatagttt ccaaaacacg gatgccgtct gcaagatttt
     4261 cagacggcat cgccctttct tggaaaaaaa caccgacatc gcgtaaaata cgcgcaaatt
     4321 ttgtgattcg gtcaggtcgt ctgaaacaga ctgcgcctga tttatttttt cggaaaaccc
     4381 tatgagcgaa caaaaccatc cgcaaaccga gccgcagttg gacgaaaacc aaatcatcgc
     4441 cctgcgccgc gaaaaactga acaacatccg ccaacagcgc aacgcttacc ccaacgattt
     4501 caagcgcgac agctttgccg ccgatttgca ggcgcaatac ggcgaaatcg gcaaagaaga
     4561 actcgacccg caggccgttc ccgttaaaat tgccggccgc atgatgctca aacgtcaaat
     4621 gggcaaagcc agcttcgcca ccattcaaga cgttaccggt caaatccagc tttatctgaa
     4681 caacaaaggc gtgagccaag aagttttgga cggcttcaac cactgggact tgggcgacat
     4741 cgtcggtgcg gaaggcactt tgttcaaaac caaccacggc gaactaaccg tgcgtgtgtc
     4801 cgacatccgc ctgctgtcca aatccctgcg tccgctgccc gacaaacaca aaggcttgag
     4861 cgatcaggaa accaaatacc gccaacgcta cgtcgatttg attgccaatg aagaatcgcg
     4921 caataccttt atcaaacgca gccaaatcat ccaatctgtg cgtaatttta tggtgggcga
     4981 gcattatctc gaagtcgaaa ccccgatgat gcacccgatt cccggcggcg cgacagcgaa
     5041 acccttcgtt acccaccaca atgccttgga cattccgctc tacctgcgca tcgcgcccga
     5101 gctgtatctg aaacgcttgg tggtcggcgg cttggaacgc gtgtttgaaa tcaaccgcag
     5161 cttccgtaac gagggcatgt ccgtgcgcca caaccccgaa tttacgatga ttgaatttta
     5221 cgaagccttc tccgactacg aacgtatgat gcagatggcg gaagacatca tccgcaacgc
     5281 atcgcgcacg gtaaacggca cggcaaacat cacttacaac ggcaaagaag tcgatttgga
     5341 aagcccgttc gaacgtctga ccatcctcga agccatcaaa aaatacaatc cgcactacac
     5401 cgacgagcag ttgaacgatg cggaatggct gaaaaaagaa atcgtcaaac acggcgaaag
     5461 cctgccgccg tccccgggca tcggcagcct gcaactcgcc ctgtttgaag gttgcgccga
     5521 gggcaaactg tggaatccga cctttattgt cgattacccg gtcgaagttt ccccgttggc
     5581 gcgcgcttcg gatacgaaac aaggtctgac cgaacgcttc gagcttttcg ttgtcgggcg
     5641 cgaactggca aacggctatt ccgagttgaa cgaccccgaa gaccaagccg aacgcttcaa
     5701 atcgcaagtg gcgcaaaaag atgcgggcga cgacgaagcc atgcactacg atgccgacta
     5761 catccgcgcg atggaattcg gtttgccgcc gaccggcggt tgcggcatcg gcatcgaccg
     5821 cttagtgatg ctgctgaccg atttgcaaac catccgcgat gtgattctgt ttccgcagat
     5881 gcgtcccgag taatcatata ttcaattaaa aacaaaatag tacaacactc
//
LOCUS       DAXDZG010000096         5238 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXDZG010000096 DAXDZG010000000
VERSION     DAXDZG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.096"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(125..2869)
                     /gene="tbpA"
                     /locus_tag="ACFB78_001900"
     CDS             complement(125..2869)
                     /gene="tbpA"
                     /locus_tag="ACFB78_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFC1269831.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDSSDYAHFIVEGECKNGGYETCKTRP
                     KKDVAGEDKRQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKYGLIRGYGKYAGDHRYGGLITKGENGAQVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSD
                     LRHQDYYYQHANRAYSPKTPPQNGDKKTIPNGGKDKPYWVSIGGGNVVTGQICLFGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEVQIKDGKEQVKGDPAYLNAQSARITGINILGK
                     IDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRDTKATARRTRPWYIVDVSGYYTVK
                     KHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
     gene            complement(2956..5034)
                     /locus_tag="ACFB78_001901"
     CDS             complement(2956..5034)
                     /locus_tag="ACFB78_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC1269832.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVPSKKPEARKDQGGYGFAMRFKRRNWYGSAKEDRVELSESDWEQTDNGNIKKPSKQ
                     QNIIDALSGNGEASLQDSSQQGEGISKVTDHHDFQYVWSGFFYKQIGSTIKKNGTSVI
                     EARNGPDGYIFYKGKDPSRELPVLGSVEYKGTWDFLTDVKANQKFTDLGNVSTQVGDR
                     YSAFSGELDYIVKKEDDKKEKHKGLGLTTEITVDFGKKTLNGKLIKNNKLINNNDEPT
                     TQYYTFDATLRGNRFSGKATATDKKENETGQHPFVSDSSSLSGGFFGPQGEELGFRFL
                     SDDGKVAVVGSAKTKDNTANGNPAVSSGAGAAAMPSETGLTTVLDAVELTLDGKEIKN
                     LDNFSDATRLVVDGIMIPLLSTESGDGQADKGKNGGTDFTYTTTYTTTYTPESDKKDT
                     KAQTGAVGMQTAPGAAGVNGGQAGTKTYEVEACCSNLNYLKYGMLTRKNSESAMQAGK
                     NSSQADAKTKQIEQSMFLQGERTDEKEIPKDENVVYLGTWYGHIATNGTSWTREASNQ
                     ENGNRAKFDVNFKDKRITGTLTAENRSEATFTIDAMIDGNGFKGMAKTGNGGFAPDQN
                     SSTGTHKVHITSAAVQGGFYGPKAEELGGWFAYPGNGQTKNAQASSGNGNSAGSATVV
                     FGAKRQELVK"
BASE COUNT         1063 a         1368 c         1317 g         1490 t
ORIGIN      
        1 cacgcctgcg cgggaatgac gggtttcggg attacggtat tgtcgggagg gatggggaac
       61 ggtgaaattt gtgtaaaaaa tgccgtctga agcctttcag acggcatttg cggcgagggg
      121 gcatttagaa cttcatttcc aagctaaatg tgtagttgcg gccgggggcg gcatatcggt
      181 tgtaaacgcc gacatttttg tgttggttga ctgcgccggc ggcagtttgc cgcacatttt
      241 cccaagtaac atagcggtgg ttgaggaggt tgtacacgcc cgcacggagg gtgaagtgtt
      301 ttttaaccgt gtaataaccg gacacatcca caatatacca agggcgggta cggcgcgcgg
      361 tggcttttgt atcgcggctg ttgccgttga gcaaagcccg gctgcccaac aactctgtga
      421 tttccttggc tttggaataa gtcagcatac cgttcacacc ccatttgcct tccggttggt
      481 catagcccga gccgacgaca tagcgcgagg gttggatggc atcaaacagg tgtgattgga
      541 tatcggtgcg gtctgcgcgt tttttaatat cgcggacacg gacacgatta taggcaaatg
      601 tggaatacca accttcgggc aatttatccc atacgccgtt ccaatcgatt ttgcccaaaa
      661 tattgatgcc ggtaatccgc gcgctttggg cattgaggta agccgggtcg cctttgactt
      721 gttctttgcc gtctttaatt tgcacttcat aaccccggac aatcaaatcg cggtaggcat
      781 tgttgaacca acttgcctcc aagttgccga aatcgccttt aaacacgatg ccggcttctt
      841 tgttgaacga tttttccgga tcgattttga cggcttttat tttatcgccc gaccgccagc
      901 cgtacatttc cgcaaacgag ggcaggcgga agccggttga ggtgcggtaa gtcaaatcca
      961 gccagtcggc aggtttgagg acgatgccgg cgttccagga cagggtgcgg tgcgtgccgg
     1021 tggaaacgct gccgtcgtcc gaatgcgtgc tgcggtagtc gtagcgcaag cccgcgccga
     1081 catccgccca cctgcccaaa cggacattgt cccgaactgc cgcgtaatag cttttgccgt
     1141 tgatgctgcg cggcgtgcag tccgtataag tattgttgcc aaagaggcag atttgccccg
     1201 taaccacatt tcccccgcct atgctgaccc aatagggctt gtccttgccg ccgttgggga
     1261 tggttttttt atcgccgttt tgagggggcg ttttcggcga ataggcgcgg ttggcatgtt
     1321 gataataata atcctgatgg cggaggtcgg aaccaaaacg gtcgtaaccg aggttgacgc
     1381 tcaggttgtg gcggattttg gcggtatcga aggatttttt gaatgccgcc tgcaagagcc
     1441 tgtggctttc cccgtaaatc acgcggtcgg atttgtagta ggaagacggc ttgtcggcac
     1501 tcgggcggca atatttgtcc gaaccgtcgg cggaacagtg cgtctgctga aaatgattgt
     1561 ccaaaccgat accctgccgg tcgtaagaga ggcgggcata atccgcccaa gtgtctttat
     1621 cggcattggt atagacatat tccaaaccgt agcggctttt ggtgtgcgtc tcgtcgtaaa
     1681 acacgcccgt accgtattcc gcgcccacct gcgcaccgtt ttcacctttg gtaatcagcc
     1741 cgccgtatcg gtggtcgccc gcgtatttgc cgtagcctct tatcaatccg tattttttat
     1801 tttcatcaaa aaccgccttg gtcaggaatg ccggaaccgt catatcgcgc gtgtcgaaag
     1861 tttgctgcgt gcgttcgagt atgccgccga tgtagtgccg cttattctca aaacgaaaac
     1921 ccgggcggaa cagccacgac cggctttcgt atgaaagcgg atcggcaagg aagcggttgg
     1981 ggcccgtgta gtctcgggtg gaaaccgttt gacgtttgtc ttcgccggca acatcttttt
     2041 tcggtctcgt tttacacgtt tcataacccc cgtttttgca ttctccttca acgatgaaat
     2101 gggcgtagtc gctgctgtca tcaaccggcg ccagcctgtt gaagctctga acgccgcgtc
     2161 cggcggcttc gtgggcgcgg atttcccccg cgtgccggcc ggtgcggatc agcaaagcct
     2221 ccgcaccgcc gatgcgcccc gccagcgcga tggattgggt aagcccccgg tttttgccgg
     2281 aataggcggt tttactctga atgccccact gcctgccttc cccgataaca tcgtcggcgg
     2341 ttttggtttg aaatgcgacc gagcccgcca atgcgccgct gccttgttcg accgagtttg
     2401 agcctttgct gatttcgaca gccttgacgt tctcatactc gatttcattg attgcgccgc
     2461 tgctgcccgc cgtcctcgtc ccgcccaatg ccgcctgcgc ggtgtaggac tgtatttgcg
     2521 ccaagccgtc caccgtcaag gagacgcggt ttttgtccat accgcgtatc gagtagcccg
     2581 agcttgcgcc gcgcccctgt tcgacgacgg cgatgccggg gtcgtaacgc gtcaggtcgc
     2641 ggatgtcgag tacctgttct ttgctgaggg tgtcggcggt tttgaccaat ttgcccaaac
     2701 cggttacttc gttatcgcgg cgggttttct gttttttggc ttttacctgt atggtatcca
     2761 actgtttttc ctgtgcttgt ccggcttgca cattttctgc ataagcgggc agcgcagtca
     2821 ttaaagacag gcataaaata ttgaatcgga acaaatgttg ctgttgcata gtgtttccct
     2881 aatcttcgct ttcagacgga atcggcagga acgatgccgt ctgaagcctt attctcgatt
     2941 gttcggcagc cgtgcttatt tcacaagctc ttggcgtttc gcaccgaata ccacggtcgc
     3001 gctgcctgct gaatttccat tgccggatga agcttgcgca tttttcgttt gtccattgcc
     3061 cggataggca aaccatccgc ccaactcttc ggctttaggc ccgtaaaaac cgccctgcac
     3121 cgcggcactt gtgatgtgca ctttatgtgt accggtgctg ctgttttgat ccggcgcaaa
     3181 gccgccatta ccggttttcg ccataccttt aaagccgttg ccatcaatca tggcgtcaat
     3241 ggtaaaggtt gcctccgacc tgttttcagc ggttaacgtg ccggtaattc ttttgtcttt
     3301 gaaattcacg tcaaactttg cccgattacc attttcctga ttggaggctt cgcgggtcca
     3361 acttgtgccg ttggtggcaa tatgcccgta ccaagtgcct agataaacga cgttttcgtc
     3421 ttttggaatt tctttttcat cggtgcgctc gccttggagg aacatacttt gttcaatttg
     3481 tttcgtttta gcatcagctt gactactgtt tttgcctgcc tgcatcgcgg attcgctgtt
     3541 tttacgtgtc aacattccgt atttcagata attgaggttg gaacagcagg cttcgacttc
     3601 ataggttttt gttcctgcct gcccgccgtt aacgcccgcc gcacccggag cggtttgcat
     3661 gccgaccgcg cctgtttggg ctttggtgtc ttttttatca ctttccggcg tgtaggttgt
     3721 tgtgtaggtt gttgtgtagg taaagtctgt tccaccgttt ttacctttat ctgcctgacc
     3781 gtccccgctt tcggtggaca ggagcggaat cataatgccg tcgacaacca gtcgggtagc
     3841 gtcgctgaag ttgtcgagat ttttgatttc cttgccgtct agtgtcaatt caaccgcatc
     3901 caaaaccgtg gtcagcccgg tttcagacgg catagctgcc gcacctgcgc cgcttgaaac
     3961 cgccggattg ccatttgcgg tgttgtcttt ggttttcgcg ctgccgacaa cggcaacttt
     4021 tccatcgtcg ctcaaaaagc ggaaacccaa ttcctcaccc tgcgggccga aaaagccgcc
     4081 gctcagagaa gacgagtcgg aaacaaaggg atgttgtccg gtttcattct cttttttgtc
     4141 agttgccgtc gccttgccgc tgaagcggtt tcccctaagc gtcgcatcga aggtgtagta
     4201 ttgggtggtg ggttcatcgt tattattgat taacttgttg tttttaatta attttccgtt
     4261 cagggttttt ttcccaaaat caaccgttat ttccgttgtt aatcccaaac ctttgtgctt
     4321 ttctttttta tcatcctctt ttttgactat ataatccaac tccccggaaa aagcactata
     4381 tcggtcgccg acttgcgtag aaacattccc taaatctgta aatttctgat ttgctttcac
     4441 atcagttaaa aaatcccaag tacctttgta ctcaacactc cctaaaacag gaagttctct
     4501 cgaaggatct ttgcctttat aaaaaatata accgtcagga ccgtttctgg cttcgattac
     4561 agatgtaccg ttttttttaa ttgtactccc aatctgttta taaaaaaaac ccgaccatac
     4621 gtattggaaa tcgtgatggt ccgtaacctt agatataccc tcaccttgtt gactggaatc
     4681 ttgcaatgat gcctccccat ttccagataa ggcatcaata atattttgtt gtttggaagg
     4741 ttttttgata ttaccattat ccgtttgttc ccaatcactc tcgctcagtt caaccctgtc
     4801 ttcttttgcc gacccatacc aattcctccg cttgaagcgc attgcaaaac cgtatccgcc
     4861 ttggtctttt cgggcttccg gttttttgga aggaacatct tgatactttg gcgcgggacg
     4921 cggggcttcg gtatcgacag aatcaagatc gaaactgccg cccccgccca gacaagcgct
     4981 caacaaaaac acaggcagca ccatagcagc ctgattcacc aatggattgt tcataacaaa
     5041 cccgattcaa ttaaagaatg ataaggatta ttattttatt tatttttaaa aaatttgcaa
     5101 atgctttttt atatttttag taaataaacc aaaataccgt cattcccgcg ctgcttttaa
     5161 aaccgcctgc aaccgccaaa cctgctgcaa tccgctccct cccctgcgcg ggggagggct
     5221 ggggagaggg cactttcc
//
LOCUS       DAXDZG010000097         5228 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXDZG010000097 DAXDZG010000000
VERSION     DAXDZG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.097"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            123..305
                     /locus_tag="ACFB78_001902"
     CDS             123..305
                     /locus_tag="ACFB78_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC1269833.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            302..1063
                     /gene="kdsB"
                     /locus_tag="ACFB78_001903"
     CDS             302..1063
                     /gene="kdsB"
                     /locus_tag="ACFB78_001903"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC1269834.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            1086..1601
                     /locus_tag="ACFB78_001904"
                     /pseudo
     CDS             1086..1601
                     /locus_tag="ACFB78_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            1758..1850
                     /locus_tag="ACFB78_001905"
     tRNA            1758..1850
                     /locus_tag="ACFB78_001905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1792..1794,aa:Ser,seq:gct)
     gene            2124..2909
                     /gene="trpA"
                     /locus_tag="ACFB78_001906"
     CDS             2124..2909
                     /gene="trpA"
                     /locus_tag="ACFB78_001906"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC1269835.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIGSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVADAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            2946..3818
                     /gene="accD"
                     /locus_tag="ACFB78_001907"
     CDS             2946..3818
                     /gene="accD"
                     /locus_tag="ACFB78_001907"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC1269836.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(3878..4489)
                     /locus_tag="ACFB78_001908"
     CDS             complement(3878..4489)
                     /locus_tag="ACFB78_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC1269837.1"
                     /translation="MRRAIFLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            4597..4821
                     /locus_tag="ACFB78_001909"
     CDS             4597..4821
                     /locus_tag="ACFB78_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC1269838.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKVLAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            4852..>5228
                     /locus_tag="ACFB78_001910"
     CDS             4852..>5228
                     /locus_tag="ACFB78_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269839.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         1201 a         1281 c         1505 g         1241 t
ORIGIN      
        1 cccgatgcgg gctgcaagca ggccggacag cttgggaaaa ggtatcagaa gtaaataata
       61 gccgcctgaa atattgcgga gggcatccga ttgattaaac catcaaaccc gaaagcaacc
      121 ttatggaaaa aaaattctta gacatcctcg tctgccccgt taccaaaggc aggctggaat
      181 atcatcagga caaacaggaa ttgtggagcc gtcaggcgaa gcttgcctat ccgattaaag
      241 acggcattcc ctatatgctg gaaaacgaag cacgagcgtt gagcgaagag gaactcaaag
      301 catgaccgaa ttcgtcgtat tgattccggc gcggctggat tcgtcgcgcc tgcccggaaa
      361 agccttggcg gacattcacg gcaaaccgat ggtcgtgcgc gttgccgaac aggcggcaaa
      421 aagcaaagcc gcgcgcgtcg tcgtcgccac cgatcatccc gacattcaga cggcctgtca
      481 ggcgcacggt atcgaagtcg tcatgacttc aaaccggcac gaaagcggca cgacgcgcct
      541 tgccgaagcc gccgccgcgc tgaagctgcc gccgcatttg attgttgtga acgtacaggg
      601 cgacgagccg ctgattgccc ccgaactcat cgaccgcacc gccgaagtac tcgtcgaaaa
      661 caacgtccaa atggcgaccg ccggccacga attgcacgat ttcgacgaat tgatgaatcc
      721 caatgccgtc aaagtcgtcc tcgacaaaaa cggcaacgcc atctacttca gccgtgcccc
      781 gattccctat ccgcgcgatg cgatgcgtgc cggaaaacgc gaaatgccgt ctgaaaccgc
      841 cgtcctacgc catatcggca tttacgccta ccgcgtcggc ttcctgcaac gctatgccga
      901 aatgagcgtc tcgccgctgg aaaccatcga atcgctggaa caactgcgcg tcctgtggca
      961 cggctacccc atcgccgtcg aaaccgccaa agaagccccc gccgccggtg tggatacgca
     1021 ggaagatttg gacagggttc gcgctgtatt tcagaccgta taaaacaggt tcaaagggaa
     1081 aagatatgca gcaacatatt gaaaagtggc aacacttgag ccgtgaagaa cagaaaatcc
     1141 ttgccgaagt atggggtctc gtgcaaaacg atgatcagga ggttcactat gaaatgctca
     1201 aattgaacgc acctgatgaa gtcagcggtg aattttggtt cagaatggca gaaacactca
     1261 gcaccctgcc gcccaatcgt tccctcgacc ttagaatgaa cggcggcagg ctgtcgaccg
     1321 ccgtatccat cctttccgtc atgattgaag acaatcccga cataccgcag ctttgggcgt
     1381 aaaaaattac cgccctcaat tatttggtac acggacacaa agcccgtgcc gacggtttgg
     1441 tacaacagcc cgacaaagcg gcagaagcca acgaagagga atacctgacc aaagccctgt
     1501 cgcaaaacct gctgtcaaca ttggatgcct cacttgcacg ttttcctgaa gacgcgtggt
     1561 ttcaggaaat caaacaggat gcacaaaagc attttgcttg aggacgtggc ggtcaggaat
     1621 atttccattc aggaagaaaa gaagtgccta attgggtata atcagggtaa atcttatttt
     1681 atttcaaaag attaatattt gttttccgtt tttccttgac ggtatcggaa aagttgatta
     1741 tagttacagc ttccttagga gtaatggctg agaggctgaa ggcacttccc tgctaaggaa
     1801 gcatgtggga tcaacctgca tcgagggttc gaatccctct tactccgcca gataaaaaaa
     1861 tagacgctgt gttttacagc gtctattttt ttatgcaatt ttatagcggg ttggcgcaaa
     1921 cccggtatgg tattgccctg tcttgattct gaattttgtt aatccgagat gtttgccgtt
     1981 tatttttgcc tcgttcaaac ggcggctctg atctgcgcgg tttctgtttg ccgtattcgc
     2041 ctatccgtac cgcaaatgtt atactgggaa aaattcaccg attgtatttt acggcgtatt
     2101 tgccgatagg atggaagaga caaatgagca gaatccggca ggcttttgcc gctttggatg
     2161 gcggaaaggc attgattccc tatattgcgg tgggcgaccc cgatattcgg acaactttgg
     2221 cattgatgca cggcatggtt gcaagcggtg cggatatttt ggagttgggc gtgccgtttt
     2281 ccgatccgat ggcggacggg ccggttattc agcgtgcggc ggagcgggcg ttggcaaacg
     2341 ggatttcgct gcgcgatgtc ttggatgtcg tcagaaaatt ccgtgaaacc gacacgcaaa
     2401 cgccggttgt tttgatggga tatttgaatc ccatacataa gatgggttat cgggagtttg
     2461 ctcaggaagc cgcaaaggcg ggggtggacg gcgtgttgac ggtggattcc cccatcgaaa
     2521 ccatcggttc tttatatcgc gagctgaagg ataacgaggt cgactgtatt ttcctgattg
     2581 cgccgacgac gacggaagac cgtattaaaa ccattgccga gctggcaggc ggatttgtct
     2641 attatgtttc gctcaagggc gtaacgggcg cggcaagttt ggatacggat gaggtttcgc
     2701 gtaaaataga gtatttgcgt cagtatatcg atattcccat cggtgtcggt ttcggcatca
     2761 gcaatgcgga aagcgcgcgc aaaatcggcc gggttgccga cgcgatcatt gtcggcagcc
     2821 ggattgtgaa agagattgaa aacaatgcgg gcaacgaggc tgccgccgtc ggtgctttgg
     2881 tcaaagagtt aaaggatgcc gtgcgctgat tctgtcgcgc attctaaatg ttttaggagt
     2941 tgtccatgag ctggttagat aaaatcctgc cacccaaaat caagaatcgt gggaaagacg
     3001 gttcttccaa tgttcccgag ggtttgtggc gcaaatgccc gtcttgttcg gcaaccgttt
     3061 attcgaccga gttgcagcag aacaatcagg tctgcccgaa atgcaaccac cacaatccgt
     3121 tatcggcgcg ccagcgtttg aacctgcttt tggatgagga aggcagggag gaagttgcgg
     3181 gtaatgtcaa accgaccgat cctttgaagt ttaaagacag caaaaaatat ccggaccgtt
     3241 tgagtgcggc acgcaagctg accggggaag atgacgcgct ggtggtgatg aaggggatga
     3301 tgaacggtct gcccgtcgtc gttgccgcgt ttgaattccg ctttatcggc ggttcgatgg
     3361 gttcggttgt gggcgagcgt ttcgtacagg gtgtccgccg tgcggttgcc gataattgtc
     3421 cgtttgtctg tgtggcggct tccggcggcg cgcgtatgca ggagggtgta aactcgctga
     3481 tgcagatgac gaaaaccagt gccgcgctgc atttgctgac ggaaaaacgc ctgccgttta
     3541 tatcggtgtt gaccgatccg actatgggcg gcgtatctgc cagcttcgca tttttaggcg
     3601 atgtcgtgct tgccgaaccg aacgcgctga tcggttttgc cggtccgcgc gtgattgagc
     3661 agacggtgcg cgaaacgctg ccggaaggct tccaacgcgc cgagtttctg ctggaaaaag
     3721 gcgcgatcga ccagattgtc gaccgccgcg atatgaagcg gcgcatcagt gatttgatta
     3781 cgctgttgtg ccgtcaggac aaagtttccg ccacctgatg gctgatgaat cgaatgccgt
     3841 ctgaaaccga tgtttcagac ggcatttttg tgtctggtta tttgtcgtgc ggcttcatcg
     3901 acggggcata gcgtccggca cgttctttca ggcgttgtac caaaccttgc gtgtcggcgg
     3961 gtacgccgcc ctcgcagaat gcctgataca ggacggcgcg caatgcgtcg ttgcgcccaa
     4021 gtgtgccgcc tatcggtttc cattcggcgt ttcggggctg tatccagcgg ctgttgaccg
     4081 tatcgccgta tccgaatact ttgtaggagg aaagtttgcc gttgccgaac ccgatggagg
     4141 agcgggcgca gaatatgcct tcggcagtca ggttgtcgta gcctttgtcg gagcggatat
     4201 tgagaatgta gcggatgctg ccgtcgggcg cgggcataat ttgcaggctg tcgagcagga
     4261 ttttcggctg tttgccgtaa ttttcatcca cataaatgtc aaaccagccg tccgagtgcg
     4321 catcgggcag cggcggcagt tcggcggtat gttctttaaa ttcgcgggcg gcggcttctt
     4381 cgggcgtttc gcggtagcgg gtgttgatcg gcgtgtcttt ttggctgaag ccggcggcaa
     4441 gggacgtgcc gacagtcagg gtcagaatca gaaagatggc gcggcgcata agtttctcca
     4501 aattgaaaac ggctttattt tatggtttgg cgggaagggc tgcaagcaat cggggtataa
     4561 tctgacctga ttttcatttt aaagcggtgt cgaaccatga acagcgaaac tttagacgta
     4621 accggattga aatgtcccct gccgattttg cgggcaaaaa aggttttggc gcaaatgcgg
     4681 cagggcgagg tattgaccgt tctggcgacc gacggcggcg cgccggggga ctttgaggct
     4741 ttttgccgcc aaaccggtca tgtgctgttg gattcttccg aacaggacgg cgtgttcaca
     4801 ctggtcgtca agcacaaata aatgccgtct gaaatgcgga tgtcctgatt gatgttattg
     4861 ctgtcggcat ttgtgcttgc tgcgtgtaat gcccaaaacc ggcaatccgg tgtacggcat
     4921 caggattttg cacgcgtagc cgtcgatttg tttcggagtg cctgcgtcct gactcaaggt
     4981 agtttcgaac cggtttccaa gtttgccgct gtcggacatt ttgtccctgt cggaaaggaa
     5041 gacctgtccc gtctgccgcc cgctgttgca gaacccgatg tacaggcgtt gtggacgctt
     5101 gaacgcgatg gcggcattta ttatttgagc ctgacgcgcg acagttgcag tgtgaaagcc
     5161 gagcgggcag acagtgccgc gcttttggaa catttctcag ggcttgtccg acagccgccg
     5221 aaaaatgc
//
LOCUS       DAXDZG010000098         4583 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXDZG010000098 DAXDZG010000000
VERSION     DAXDZG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.098"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            87..536
                     /locus_tag="ACFB78_001911"
     CDS             87..536
                     /locus_tag="ACFB78_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC1269840.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(913..1059)
                     /locus_tag="ACFB78_001912"
     CDS             complement(913..1059)
                     /locus_tag="ACFB78_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269841.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            1476..2024
                     /locus_tag="ACFB78_001913"
     CDS             1476..2024
                     /locus_tag="ACFB78_001913"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1269842.1"
                     /translation="MNAKFIQADIFELQPETLEKELGLKKNDDDLILIGCSPCQYWSV
                     IQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGILSRMKESGLDNFIKLLEEKG
                     FTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSSIRANGLRYAMFWEWKTAFPK
                     LWQDIKTRRILCIVVRDYPISI"
     gene            2021..2392
                     /locus_tag="ACFB78_001914"
     CDS             2021..2392
                     /locus_tag="ACFB78_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1269843.1"
                     /translation="MKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWW
                     DKPAPTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIG
                     NAVPPMYAEKIGRAIVDNIEC"
     gene            2406..3263
                     /locus_tag="ACFB78_001915"
     CDS             2406..3263
                     /locus_tag="ACFB78_001915"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1269844.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            3453..4049
                     /locus_tag="ACFB78_001916"
     CDS             3453..4049
                     /locus_tag="ACFB78_001916"
                     /inference="COORDINATES: protein motif:HMM:NF021057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC1269845.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            4055..4477
                     /locus_tag="ACFB78_001917"
     CDS             4055..4477
                     /locus_tag="ACFB78_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC1269846.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
BASE COUNT         1525 a          727 c         1029 g         1302 t
ORIGIN      
        1 gtgggaaata tgcatactgc tgaatgggat agtaagtctg ttcaaagaaa tatgttgaat
       61 aatctgttct tattggaagt aaagtaatga ctgataatcg ggggtttacg ctggttgaat
      121 taatatcagt ggtcttgata ttgtctgtac ttgctttaat tgtttatccg agctatcgca
      181 attatgttga gaaagcaaag ataaatgcag tgcgggcagc cttgttagaa aatgcacatt
      241 ttatggaaaa gttttatctg cagaatggga gatttaaaca aacatctacc aaatggccaa
      301 gtttgccgat taaagaggca gaaggctttt gtatccgttt gaatggaatc gcgcgcgggg
      361 ctttagacag taaattcatg ttgaaggcgg tagccataga taaagataaa aatcctttta
      421 ttattaagat gaatgaaaat ctagtaacct ttatttgcaa gaagtccgcc agttcgtgta
      481 gtgacgggct ggattatttt aaaggaaatg ataaggactg caagttactt aagtaggctt
      541 gtttacaaat cgatcgatag tgtttcagta tttgtgcggt tttccgcctt gatggaatga
      601 gaatcgtgta atattaagcc tatagttatg acccgtgatc cgtatctgga ttgtttctgg
      661 ataaaagaat aaacccaatt atctttagcc gtggatttat ggtgcatggt agcaaaaatt
      721 gtataggggt gtacattact catctgaaaa ggtgaacaga ttttataatg ccgtctgaag
      781 atgttttcag acggcatttt gtttccaatt cgttataggc ttagtaattt aaggcatgtt
      841 tgcaccttta gacataaggg caatcgcagc cccgtcctta atgtacaaat cactatccag
      901 cttcaaccat gcctaatagt tttccgaatc gaataccttc gcccgataac gccctaagga
      961 aattttttgg acatcgcgaa ccttgtcctg ctgaaaatac aaaaggccgc gttcgacgat
     1021 gtggggcgga aataggaggg gtaggtagat acgggacatg gtttgcttcc aaggagttgt
     1081 tgaaatatgg ggaatttact ggtttgggcg atgtttgggg agaggggtgg gaattcttgt
     1141 tttgcgaagt ggaaaaaggt cgtctgaaaa tgggtttcag acgacctttc tgtgaaatgt
     1201 gcatttgaag atatccgatc gtttggagga aattaagttc tgcttattta gatgcatgaa
     1261 ttttaataaa atctcaaatt atttttgata agcaataaat tatatgttat ataaagttag
     1321 ttctcactag tctaattatt taaatagaaa tgaaaaacag taagttaaag gcggttgatt
     1381 ttttctgtgg cggaggcgga atgagctatg gaatgtaatc cgctttagcc gggatagatt
     1441 acgaacttag ctgcaaagaa acatatgaag ccaatataaa tgccaaattt atacaggctg
     1501 atatttttga attgcagcct gaaaccttgg aaaaagaact cggcttaaaa aagaatgatg
     1561 atgatttgat tcttataggt tgtagtcctt gtcaatattg gagtgttatt caaacagaca
     1621 aaagaaaatc ggaaaaatcc aagagtttgc ttttggagtt tcagcggttt gtagaatatt
     1681 tcaatcccgg atatgttgtt gtagagaacg taccgggaat tctgagcaga atgaaagaaa
     1741 gcgggcttga taatttcata aagttattgg aagaaaaagg atttaccgtc catttcggta
     1801 ttcacaatac ggctgattac ggaattcccc aaagccgtaa aagatttacg ttaattgcaa
     1861 acagaataac caaaaaaagc tggaaccagt caagtattcg ggcaaacggc ttacggtacg
     1921 cgatgttttg ggaatggaaa acggctttcc caaaattatg gcaggacatc aagacgagac
     1981 ggattttatg catagttgtg cgggattatc cgatatcaat ttgaaacgat tggctttgat
     2041 acctaaaaac ggaggaaacc gtttggcttt tgcgcatatt cccgaattac agctggaatg
     2101 ttttatcgga aaagacaata gttttaaaga cacgttcggt aggctatggt gggataagcc
     2161 cgcacctacc ataacgacca aattctttag catttccaac gggcggtttg cccatcctga
     2221 agaagatagg gcgttatccc tgcgagaagg tgcaacattg caatcgttcc ctcgtaacta
     2281 tgtctttaaa gcgggcagta gggacaagat agcccgtttg attggtaatg ccgttcctcc
     2341 gatgtatgcg gaaaaaatag gcagggcaat tgttgataat atcgaatgtt agaaaaggag
     2401 aaaacatgtc tgaagaaaaa ttgaaaatga gtttcgagcc aaccgtaatc gaacatttgg
     2461 gtgtaaagat gtattcgcac actgttcctg cgattgccga gttgatagcg aatgcctacg
     2521 atgcatgtgc tacggaagtg gaagttaggt tattcgataa accggagcat aaaatcgtta
     2581 ttaaagataa tggcatagga atgagcttcg atgaaatcaa tgatttttat ttgagaatcg
     2641 gtcggaacag aagggaagaa aaacaagcct ccccgtgcgg aagaattcca acgggtaaaa
     2701 aaggtcttgg taaattggca ttattcaggc ttggcaacaa aatcgaaatc tctactatcc
     2761 aaggaaacga acgggttact tttactttgg attatgcaga gattaaaaaa agtgagcgta
     2821 tttatcaacc ggagtttcag aaagagtctg ttaaacccaa taccgaaaac ggaacaacta
     2881 taactttaac cgagctgacg aaaaaacaag gatacccgtt agataattat gtggggcatc
     2941 tttcccgttt atttgatttt ccggctcagg attttaaaat caaagtaagc ttgaacggct
     3001 cggaaccaag aatcattgac ggaaacctaa aatataatct tgttacccca caattcgaat
     3061 gggaatacca ggatctagca accaatattt catcgttatc ttcaaaattc gaacagtatg
     3121 aatacagcgg attaatacaa ggtaagttca ttacaacgga aaaaccttta aagaataata
     3181 tgaaaggtat taccttgttt gccaacggca gaatggtaaa tatgcccgag tttttcactg
     3241 atagcgaatc cagccatttc taatcctatc tgacaggatg gttaagtatt gattttatag
     3301 ataatgatgg tgaagattta atcgctacag ataggacatc attggattgg aagcatccta
     3361 aaacttcaaa attacgggtt ttccttgggg aaatcgtctg agccatcggg caagattgga
     3421 gaaaaagaag aaaaaaagag aaagattacg aagtgaaagg tgaaagcgga gttgatatcg
     3481 aaaattggaa aaacaaattg ccagaaaagg aaagagaacc ggtagaagtt attttgaatc
     3541 gtttggaaga ttcggagctt acaaataaag aacaagcgga agtaatctct gccttacata
     3601 gtattattcc agaatatcct tactatcatt ggcggcattt gcatcaggat ttgcatactg
     3661 cctgtaacga tttttacaat gaaaagaagg attatttaag tgctgcaatc gaggcagtta
     3721 aagttttcga agataaggtt cagaagcaga caggtcttca tagcatagac ggaagagaat
     3781 tgattgaaaa agccttcggg agtaaaaaat ctatgcttct attaacgaat aacaaaacgc
     3841 aggcggaaca aaatttagaa gatggtcttg aacagcttgc atgcggaaca tggactggat
     3901 ttagaaatcc tgtgcaacat gagttaagag caaatttatc tcctagtatt tttaatgaca
     3961 aggatgcttt ggatttaatc agcttagttt catatttatt gagaaaagtg gaacaaacaa
     4021 aaaaacgagc taaacctaca tcgccttaaa ttttatgacc gatattttca ctccatccaa
     4081 acgttccttc gtgatgtcaa aaatccatag caaagaaacc aaaccggaag tattggtaag
     4141 gaaatttctt ttttcccaag gtttccgtta tcggaaaaac gataagcgct atgcaggcaa
     4201 accggatatc gttttgccta aatataaaac agtcgtgttt atacacggct gtttttggca
     4261 cgggcattct tgtaataaag gacatatccc gaaaagcaat atggattttt ggttggaaaa
     4321 aatcacaaaa aatcgcgaac gtgatattaa aaatgaaacc gagctggaaa agattggttt
     4381 caaagtgatt gtcgtttggg agtgcgaatt aaaaaataaa gctatttgta gggagcgatt
     4441 aaatcggttg gtcgaggaaa taaaagatgc cgtctgaaaa aatcagacgg catcttcctt
     4501 taattaagac ccttatatat tgcgtcccta agaaggggtg attaacaaaa attaacgccc
     4561 tttactttct acaagtaaca ggg
//
LOCUS       DAXDZG010000099         4507 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXDZG010000099 DAXDZG010000000
VERSION     DAXDZG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.099"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFB78_001918"
     CDS             complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFB78_001918"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC1269847.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFB78_001919"
     CDS             complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFB78_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC1269848.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1495..1872
                     /locus_tag="ACFB78_001920"
     CDS             1495..1872
                     /locus_tag="ACFB78_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC1269849.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     TALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1882..2691
                     /gene="dapB"
                     /locus_tag="ACFB78_001921"
     CDS             1882..2691
                     /gene="dapB"
                     /locus_tag="ACFB78_001921"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC1269850.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIESHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGNRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2805..3074)
                     /locus_tag="ACFB78_001922"
                     /pseudo
     CDS             complement(2805..3074)
                     /locus_tag="ACFB78_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3165..3323)
                     /locus_tag="ACFB78_001923"
     CDS             complement(3165..3323)
                     /locus_tag="ACFB78_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269851.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3331..4503)
                     /gene="lpxB"
                     /locus_tag="ACFB78_001924"
     CDS             complement(3331..4503)
                     /gene="lpxB"
                     /locus_tag="ACFB78_001924"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC1269852.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
BASE COUNT         1121 a         1457 c          994 g          935 t
ORIGIN      
        1 tccactatat ttgaacctga ccgaagcata agccgccatc aaagcccctt gcacgccact
       61 tcgcgccgcc cgatttcctc tttcaacggc acggcgttca acatccgcag acgttcggca
      121 aaatccgcaa acggcagcag ctccgaaccg aaacggcgca tatgttccgt gtcctgctgg
      181 cagtctatca gttccacgcc caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt
      241 ttcgacgcat ccggttgcaa cgcaaacatc gattcgccat aaaacaccct gccgatctga
      301 acgccgtaaa agccgcccgc caacctcgtt ttaccgtttt catcgggata atggcactcg
      361 aaagaatgcg cgtgtcccat ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca
      421 atccaagtcc catcctgatt cgggcgcgcc gctgccgcac aatgcgcgac aacttccgca
      481 aaacagccgt tgaccgcaac ccgatagctg ccgttacgca gcgttttcgc cagcgagcgc
      541 ggaacgtgca gacgttcggg aacaattacc gcacgcggcc cgaccgcata ccaaaaaaac
      601 caaccgtccc gcgaaaacca aggaaacacg ccgttccgat acgcctcgag cagccgcccc
      661 gcgtccaaat ccctgctcac gccgaccagc ccgtcgcacc gggccaaagc ataggcagga
      721 tcgggaaagg cataattgtc aggggcaagc agcggaatac gcatggcagt tctccaaaaa
      781 cacagacggc atccaaaatc atgaatgccg tctgaacaac aaaccgtccg gatttaacgt
      841 ttgcccttgg cctgacagtc gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg
      901 cggtagccgt tttcctcggc gattttgtct tgcagggctt cgatttcggg attgtggaat
      961 tccgttacct cgccgcactt cacgcagacg atatggtcgt ggtggtcgcc cttgtccaac
     1021 tcataaaccg ccttgcccgt ttcaaaatgg tggcgttgca gaatgcccgc ctgctcgaac
     1081 tgcgtcagca cgcggtaaat cgtcgccaca ccgatttcca cgccctcttc cagcagaatg
     1141 cggtacacat cttccgcact caaatgctct tccgcgtgct tctcgaacaa atccaaaatc
     1201 ttcaaacgcg ggccggtaac cttcagaccg ctgtctttca gttgcgcaat gttgctgaat
     1261 ttttccataa tattcaatat ccctgtaaaa taatagacgc tataatacgc aatttcagcc
     1321 tgcttgccca ctatcacacc atagcagttc gcaatagcaa aaccgcaacg gcggacgggc
     1381 agtcggacac agacagggta aaataccgct taagcgtatg attatcgttc gcttttataa
     1441 aatattcaag cagtgctaca ctagacatcc cgatttgcac agaaaggttc tcccgtgaac
     1501 aaaaccctca tcctcgccct ttccgccctg ttcagcctga ccgcgtgcag cgtcgaacgc
     1561 gtctcgctgt ttccctccta caaactcaaa atcatccaag gcaacgaact cgaaccgcgc
     1621 gccgttaccg ccctgcgccc cggcatgacc aaagaccaag tcctgctcct gctcggcagc
     1681 cccatactgc gcgacgcttt ccataccgac cgctgggact ataccttcaa cacctcccgc
     1741 aacggcatca tcaaagaacg cagcaacctg accgtctatt ttgaaaacgg cgtactcgtc
     1801 cgcaccgaag gcgacgccct ccaaaatgcc gccgaagccc tccgcgcgaa acaaaacgca
     1861 gacaaacaat aaggaacaca catgataccg ctcaaaatcg ccatcgccgg cgcaaacggc
     1921 cgcatgggac gcgtcctcgt cgaagccgtc aacaaccatc ccgacaccgt cctttccggc
     1981 gcgctcgaac actcaggctc agaagccctc gggctggacg cgggctacgc cgtcggactc
     2041 aaaaccggca tcgccatttc agacgacgtt gacgccgttc tcgcccaaag cgacgtactc
     2101 atcgacttca cccgccccga gccgaccctc aagcacctgc aaaaatgcgt tgaaaaacaa
     2161 gtcaacatca tcatcggcac aacaggcttc gacgatgcgg gcaaagccgc catccgcgcc
     2221 gccgccgaaa aaacaggcat cgttttcgcc gccaacttca gcgtcggcgt caacctcacc
     2281 ttccacatcc tcgacaccgt cgcccgcgtc ctcaacgaag gctacgacat cgaaatcatc
     2341 gaaagccacc accgccacaa agtcgatgcc cccagcggca cagcattacg aatgggcgaa
     2401 gtcatcgccg gcgcgctcgg ccgcgacctc aaacaatgcg ccgtttacgg ccgcgaaggc
     2461 cacactggtc cgcgcgatcc ttccaccatc ggctttgcca ccgtccgcgc aggcgacata
     2521 gtcggcgacc acaccgccct cttcgccacc gacggcgaac gcgtggaaat cacccacaaa
     2581 gccggcaacc gcatgacctt tgccgccggt gccgtccgcg ccgcagtttg ggtaaacggc
     2641 aaaacgggtt tgtacgatat gcaggacgta ctcgggctga acaaccgtta acccccatac
     2701 aaaatgccgt ctgaaaagat attgttcaga cggcattttg ccgacaggct tcgtatcggc
     2761 atatcaataa agttgcttgt gcagcatgac aagctacggc tcgtttaatt gaaactctcc
     2821 tgatctaaaa attccaactc tatttcccgg caaactatat ctatgataaa actggtcgga
     2881 agattatacc tacgccctat taatgccagt gcacctccat aatccaagat ttcatcatcc
     2941 tgctttaatt ctccaagctc caaggtatta tcgatatctt tatcttggtt ctccgtagcg
     3001 tatccataga taaatttcag atagctgaac tgcttgaatt taatttttac ccagtctgtg
     3061 taatttggtg taggacacaa ttgcctaaaa tagaaattta cgaagaacaa atgaaacaag
     3121 gtacgtaagc gtattagtat aaactataat catatataat aaaattaatc agttaacgaa
     3181 ccattatatt ttaaccttaa tcccattcgg gccagttctg tttcatttgg aattcctctt
     3241 aaatacttat tgtttgatgt gccattaatc acaatttctc cccctttttt tgtaatacgt
     3301 gctgcttcgg gaaaataatc cattaaccgc tcaacatccc gcctcttcca aaaccgcgcg
     3361 cgcggccaaa tccgccgtat cttttttcaa cagcaggtgc aacgccccga aatcctgttg
     3421 cagcgcggca accttatcgg ggtgttcgta ccagtccgcc aatgccgccg ccagtttttc
     3481 cggttttgct tcagattgca ataattccgg cacggcctcc ttacccaaca ggatattcgg
     3541 caggccgaca tgcggcactt tgattttgcg tttcacataa gcataggtca gcggcgaaat
     3601 cttgtagctg atgaccatcg gacgcttgca caacgccacc tccaaagtcg ccgtaccgct
     3661 cgttaccagc accgcatccg ccgccctgca caccgtttca gactgtctgt cggttaccgt
     3721 cagcgccaat ccggcaaact ccggccgctg caaaacttcc gccaaacgcc gcttcgtcgc
     3781 ctccgttgcg gcaggcagca ggaagcgggc ggcgggatag cgttccaaca acaataatgc
     3841 cgtctgaaaa aacaccggcg ccatatagtc gatttcgctg acgcggctgc cgggcagcag
     3901 ggcgaatacg gggatgccga catccgcgcc caaagttttc cgcgccgttt cacggtcgtc
     3961 ttccaagggc ataagctgtg ccatcggatg accgacaaac tccgcacgtc cgcccgcatc
     4021 gagataaagc tgcggctcca tcgggaacag gcacagcacg cggttgacct gatgcacgat
     4081 tttgcccacg cgttcgcgcc gccacgccca aaccgacgga ctgacataat gcagggtcgg
     4141 aatgcccgcc cgtttcagct tttccgccac acccagatta aaatcgggcg catcgatacc
     4201 gacaaagaca tcaggtttca acgacagcaa atcccgtacc agctccctgc gtatccgtaa
     4261 aatttccggc agccgcctga ccacttcgac aaagccgcgc accgccagcc gctcctgatc
     4321 ataaaggctc tcgaaacctt ccgccttcat cagttccccg ccgatgccgg ttaaccgtgc
     4381 ctgcgggcaa cgctttcgga tggcgcgtat caggtgcgcc cccaacaggt cgcccgacgc
     4441 ttcgccgaca ctgacggcaa tcaaagggct ttttttatca accatattcg tctgtcccca
     4501 catatag
//
LOCUS       DAXDZG010000100         4419 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXDZG010000100 DAXDZG010000000
VERSION     DAXDZG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.100"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            104..883
                     /gene="yaaA"
                     /locus_tag="ACFB78_001925"
     CDS             104..883
                     /gene="yaaA"
                     /locus_tag="ACFB78_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC1269853.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGGIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNVKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNENEWVFMRSEQIK"
     gene            966..1056
                     /locus_tag="ACFB78_001926"
     tRNA            966..1056
                     /locus_tag="ACFB78_001926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1000..1002,aa:Ser,seq:tga)
     gene            1133..1219
                     /locus_tag="ACFB78_001927"
     tRNA            1133..1219
                     /locus_tag="ACFB78_001927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1167..1169,aa:Ser,seq:cga)
     gene            1413..2605
                     /locus_tag="ACFB78_001928"
                     /pseudo
     CDS             1413..2605
                     /locus_tag="ACFB78_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2961..3230)
                     /locus_tag="ACFB78_001929"
     CDS             complement(2961..3230)
                     /locus_tag="ACFB78_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269854.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(3425..4108)
                     /locus_tag="ACFB78_001930"
     CDS             complement(3425..4108)
                     /locus_tag="ACFB78_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC1269855.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            complement(4136..>4419)
                     /locus_tag="ACFB78_001931"
     CDS             complement(4136..>4419)
                     /locus_tag="ACFB78_001931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269856.1"
                     /translation="AAKVRRYCGEHPGVFDGAAGSRQLERYIKPSEFHAVEIQPEACK
                     ALLQNYPAAAGNLGRRKQKSPEFVSVSALAEWPDRIESKAKDEWARVRN"
BASE COUNT          995 a         1010 c         1157 g         1257 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatactt cacttttaat cggcttgccc gcaaacacgt
       61 ttaaacttaa aatccccatg tttgacacta taccgagcag attatgtttt ttgttctttc
      121 ccctgcgaaa aaccttaacg aaaaagaccc ttgccctgtc agcgagttta cccaacccga
      181 cctgctggca gaatccgaaa tcctgatgcg ccaactgagg gagcttgcgc cgcaacagat
      241 tgcagagttg atgcacgttt ccgacaaaat cgcacttcta aacgccgaac gaaatgcagc
      301 gtggcacacg ccgtttacgc cggaaaacgc caaacaggca gtctttatgt tcaacggcga
      361 tgtttacgaa ggtatggatg caaacacatt gaataccgat caaatacaat atctgcaagg
      421 gcatgtacgc ctgctgtccg gtttgtacgg tcttcttcgc ccgttagacc tgatacagcc
      481 ttaccgtctg gaaatgggaa cggcatttgc caatttgcgc ggcaagaacc tgtatgagtt
      541 ttggggcggc atcattacca accttttaaa tgacacgctt gcccaagcgg gcagcaatac
      601 gcttgtcaat ctcgcctcac aggaatattt caagtctgtc aatgtaaaaa aacttcgggc
      661 gcggctgatt accccgatat ttaaagacga aaaaaacggt aaatataaaa ttatcagttt
      721 ctatgccaag cgcgcgcgag gattgatggt gcgctatgcg gcagaacaca atattaccga
      781 tcctgaaatg ctgaaaaatt ttaattatga aggctacgca ttcaatgacg cggcttcaaa
      841 tgaaaacgaa tgggtgttta tgcgttcgga acaaataaag tgaaaacaat aaattaagta
      901 tttcccaaaa aaagtgcttg gcaaaatgta taaacttcat tattattcct actcgtcaag
      961 aagacggaag cgtggcagag cggtttaatg caacggtctt gaaaaccgtc gagggttgat
     1021 agccctccgt gagttcgaat ctcaccgctt ccgccaattt ttgagcgtaa aaccaaataa
     1081 aaatgcaata gccgcgaata ttgttatttt tatttggttt tactgcatta tcggaaacgt
     1141 ggcagagagg ctgaatgcag cggactcgaa atccgctgag ggtgcaaatc ctccgtgggt
     1201 tcgaatccca ccgtttccgc cgcaaagcaa aaccgccctg attcagggcg gttctttttt
     1261 gttcaagttt tgttgattac catataaaaa tcatcggctt gacctatcat aacgcttcaa
     1321 ggcaaatcat ttgcaatctt gcggcatctt cttattgcat ttttttatgg taatgtatat
     1381 ggtaattttt ggataagtgg gaaattacca taatggcgaa aatcattacg ccgctgtcgg
     1441 caaatcaggt taaaaatgcg aagccgcgcg ataagctgta taagttgtcg gacgggggct
     1501 ggctttgtgg gtctacccga cgggcggacg gagttggaag ctgtcgtttg tgcaggatgg
     1561 aaggcagcag acaatttcgc tggggcggta tcctgatttt tcgctgaccg atgcgcggga
     1621 atggcgggag gaggtgcgcc gaaaacgggc acacggggaa aatgtcgtca ataagaaggt
     1681 gcgggcggat tttgcttttg agaaggtggc gcgtgattgg tttgtgcgtt ggtcgaaggg
     1741 gcggtctgaa aagtatgccg gacaggttat gcggaatttt gaacggtggg tttttccggc
     1801 tatcggcaat cttgatatcc gtcaagtcag gacggcggac gtggtcggct gcctgcgtgt
     1861 gatggaggcg cgcggtatcg ttgatacgtt gcgcaaaacg aaaaacagtc tgaagatggt
     1921 gtttgcgttt gcggtcggtt cgggaatgat ggaaatcaac cctgtcgcgc aaatcggttc
     1981 gggtgtgttt gaacgggtga aaaccaaaaa catgacggcg ttgagtccgt ccgaattgcc
     2041 gcgcctgatt gattttttgg agcagcgcaa tgaatttgcg gtttatgcgg gcagggtgca
     2101 tatccatcct gtaacgcggt tttgtatcta ttggctgctg ttgacgatga cgcggattcg
     2161 ggaggcggcg ttgatggagt ggtcggagtt ggacggggag gtttggcgta tccccgccga
     2221 acggaaaaag gagcggcggg ggcatgatgt gccgctgtcg cgggcgatgc ggtgggtgtt
     2281 ggatcaggcg cgggggctga atgtgaatgg gcggtttgtg tttgaaagtg tgaattttca
     2341 agggcatatc aataaggaaa gtccgcgcgt ggcgatgcgg cggtcggggc tggatacgac
     2401 ggcgcacggt ttgcgctcgc ttgcgcgtac ttatttgcgc gaggttctga aggtgtataa
     2461 tgatgtggcg gaaaagctgc ttgcccattc gttggggacg aggacgcaaa cggcttacaa
     2521 ccgctcggag ctttgggagg agcgtaagga tgcgccggaa cggtggggga atgatgtttt
     2581 gaggcttgcc gacaacggaa aatgattttt ttgtgtgttt tgcgttatgt ggaaatgtag
     2641 ataagaaagc tgtttccgca ttatgtgaaa acgccctaat cgggcgtttt tttattgctg
     2701 caaccctaat gcttccaaaa cttcgcgggt gtcccatacg ggggatgcga ggggggttca
     2761 gacggcacgg tatccgtccg tctttttcca tacgcagcag tgtcgagttt gaaatggggt
     2821 ggttgcggca ggttgcgtag gcaatcagtt cgcggatggt cgggcggtct atccttgcgc
     2881 ccggtttggt gatgttcatt tttttactct cctgttcggt ttaatcttgc gccgttttta
     2941 cggctctgtc gtttgtctgc ctacttcttc ttcccgattt ttttatatcg caacaagctt
     3001 tccggcggga agaatatgtc aaagtctgtt ttcaaatcgg gactgacgat gtcggcggca
     3061 atcacgccgt agccggtggc aaagctgtcg ccatcagcgt tatgaataac tatatgcccg
     3121 cttttgatta gaataacgcg gccgaatccg gatttccgga ttccgccttt tttcggggcg
     3181 gttgcaaatg ccagtgtttc catccgccct tgtacgtcgg aagactccat tgcttcgccg
     3241 accgcttttt tgacgggcgt ttattgaata cttcgtattc catgtcttca atcctctttc
     3301 cgttgattct gatttgtccg ggtttccgct tttttacggc ttggcatgcg gcaattgcgt
     3361 ctttcatatt cggcatatcc tttccccaat ccgccgaagt ttccgcatcc gctgccgtct
     3421 cgattcatgt ttgccctccg atttgtttta cgaagcccgt accgttcacg gcatagtgta
     3481 acttcacttg cttacccgat tccattcctc gggttaatgc ctcatattcc atttttgacc
     3541 cttgtaggat tgacgatttc gcatctcttg tccttgcttc ggccatatcc ggatattctt
     3601 tctttttgta tccgtccata atggcttttt cttcatctga catctcaaat tttttgactg
     3661 tttcccaagc gctcgcaatc caaccgccgc aaaatcggtc ggcaagataa gttctgtgcg
     3721 agggctttcc cgcccggcag gttttcaaaa atttgcggcg gtcggcggaa atctgacgat
     3781 agactacatc aaaggcatag gctgaggttt ccgctcgatt tccaatgccg taaaacatca
     3841 ttgttttccc gcgttggtaa gatttgcacc cgaatatatc ggcaatcata tttgcaacgc
     3901 cccattgcca gcctgccaat ttaaaagcca ttttccgatc ggctttctgt tcggagactt
     3961 ttgataaggc gatatcctcg gcatttactt tgtacttttc cattaaagcc tgcgcctgcc
     4021 ttaacgcctg tgcggcttca tgttcatttg ccgaccgacc caaagccaaa catttcttga
     4081 ttttgtctaa gactttctct ttatccatat taaacccttt aatttgtccg gtattttaat
     4141 ttcgcaccct tgcccattcg tctttcgctt tgctttcgat tcggtcgggc cattcggcta
     4201 gggctgatac gcttacgaac tcggggcttt tttgtttccg cctgccgaga ttaccggccg
     4261 cggcagggta gttttgcaac aaggctttgc aggcttcggg ctgtatttcc acggcgtgaa
     4321 attcagacgg cttaatatac cgttcaagct gcctgcttcc tgccgcgccg tcaaatacgc
     4381 ccgggtgttc gccgcagtat cggcggactt tggcggcaa
//
LOCUS       DAXDZG010000101         4365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXDZG010000101 DAXDZG010000000
VERSION     DAXDZG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.101"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(69..2045)
                     /locus_tag="ACFB78_001932"
     CDS             complement(69..2045)
                     /locus_tag="ACFB78_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC1269857.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2191..3030
                     /locus_tag="ACFB78_001933"
     CDS             2191..3030
                     /locus_tag="ACFB78_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC1269858.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3229..4260
                     /gene="gap"
                     /locus_tag="ACFB78_001934"
     CDS             3229..4260
                     /gene="gap"
                     /locus_tag="ACFB78_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC1269859.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1095 a         1473 c         1024 g          773 t
ORIGIN      
        1 tgcgatttgc gaaaacgcta ttagaccaaa taaccgggaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caagctccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcccaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ccatatcggt aaacgttttc acataatcgt cattggtggc gcgcgcatat
      541 acgggcatat tgggatgcat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggc tcgcctgcac ttctggctac ctgcacgcgt
      721 gcgatgtcca agtcgagcgc gaaatacgga atatcctctt gggcgaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatgat cagacttgct catcgcttcc
      841 accaacatgc tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caagcgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccaa ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgct tcattttgaa tgcaatggca aacacaacca atgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa gaagccgagc aaaatatcgc ggaacgggcg aatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcaacatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgccagcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaact ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg atacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca gcttgggcaa tgaaaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaga acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatggacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggtg cggcatacaa ttctgataaa
     2161 atacgccctt ttgacccgca ccaccgcaca atgaccgcca ccgccgccga tttagaccga
     2221 ctcctccccc aaacccaatg ccgcgaatgc ggctatgaag gctgcctgcc ctacgcccgc
     2281 gcgatgctgc ggggcgaggc gcacaacctc tgcgccccgg gcggcgcaac cgtcgtccgc
     2341 gaccttgccg ccctgctcgg caaacccctt gtcgcacctg ccaaaaccca agccaaagca
     2401 ctcgcccgga tagacgaaac cgcctgtatc ggctgcaccg cctgcatccg cgcctgccct
     2461 gccgatgcca ttatgggcgc gggcaaactt atgcacaccg tcatcaccga cgaatgcacc
     2521 ggctgcggac tctgcgtcgc cccctgcccc gtggactgca tccatatgca gcccgttgcc
     2581 gacaccgtcc tgccgcgcgc gcgccgtttc agcctgtccg acgacagccg ttttgccgcc
     2641 gccgaacacg cgcgtgcgcg ctacctcaaa cgcaacgaac gcaaacagcg cgaagccgac
     2701 gaacgcaagg ccatgcttgc cgaacgcgaa gccgccgtcc gcaacgcgcg tccgcaaacg
     2761 cccgacacac cgaaaaaacc ggcgttcaac cccgccgacc tcatcgccaa agccatggca
     2821 aaagcgcaaa cccaacagga tcgcctcgcc gccgccgaca accgccaagg ctatcaggca
     2881 aaacagatag ccgaagcccg cgaacgcgcc gagttgcgcc gcgcccaacg cgatatgaaa
     2941 tacggcagcg acagcgaaaa agccgccgcc ctcgaatacc tcaaacaata caaagccaaa
     3001 caggaagccg cacagaatac cgcctcctga cccttacccg acatgccgtc tgaagccgct
     3061 tcagacggca ttttatcaag ctctccgtcc gccgtacccg tgccgtccgt atcttaccgc
     3121 ccacccttcc ggccgtgccg ttttcaataa aatattaatt acacgccact acaaatttgc
     3181 tataatccgc cccgaaaatc tacccaaccc tcaacaaagg aacaaaccat gggcatcaaa
     3241 gtcgccatca acggctacgg acgcatcggc cgccaggttt tgcgcgccat ctacgactat
     3301 cagattcaag accaactcca aatcgtcgcc gtcaacgcca gcggcagcct tgaaaccaac
     3361 gcccatctga ccaaattcga caccgtacac ggacgctttg aagccgacgt atcccacgac
     3421 ggcggcaacc tcatcgtcaa cggcgacaaa atccccttct tctcgacccg caaccctgcc
     3481 gaactgccgt ggaaagaact cggtgtcgat ttggtcatgg aatgcaccgg cgcgttcacc
     3541 agcaaagaaa aagccaaaat ccacctcgaa agcggcgcga aaaaagtcct catttccgca
     3601 ccgggcggcg acgatgtcga tgcaaccgtc gtgtacggcg tgaacgacag cgtcctgacc
     3661 gccgacatga ccgtcgtttc caacgcttcc tgcaccacca actgcctctc gccggttgcc
     3721 aaagtgttga gcgaaagcgt cggcatcgtc aaaggcgcaa tgaccaccat ccacgcactg
     3781 accaacgacc aaaccgttac cgacgtgcgc cacaaagacc tgcgccgcgc ccgcagcggc
     3841 gtggaaaaca tgattccgac caaaaccggc gcggcaaaag ccgtcggttt ggtcttgccc
     3901 gaattgaaag gcagactcga cgggcttgcc atccgcgtgc cgaccgtcaa cgtatccttg
     3961 gtagatttga gcttccaagc cgcgcgcgac accacagtcg aagaaatcaa cgcactgatg
     4021 aaagccgcct cggaagcagg cgcgctcaaa ggcgttttgg gctacaacac cctgccattg
     4081 gtttccatgg acttcaacca caccaccgaa gccagccact tcgacgcaac gctgaccaaa
     4141 gtcgtcgacg gcaacatggt caaagtgttc gcgtggtatg acaacgaatg gggcttcagc
     4201 tgccaaatgc tgaacaccgc acgccgtatg ttcggacttg aagtgcgccc gctcaaataa
     4261 gcgacaaacc gtcaaacaaa atgccgtctg aaacccgatg ttttcaagtt tcagacggca
     4321 cttttcattt ttgaccgcga ttttacccgg ctgtcgtcat ttcca
//
LOCUS       DAXDZG010000102         4278 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXDZG010000102 DAXDZG010000000
VERSION     DAXDZG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.102"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..218
                     /locus_tag="ACFB78_001935"
     CDS             <1..218
                     /locus_tag="ACFB78_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269860.1"
                     /translation="IAPRLLRVLCVWRVRRREICLLFPRRAGGTGGTLSINRNTVYND
                     RLFHHIPERNNVSTHGTAHQAPPYVSS"
     gene            316..1236
                     /gene="ribF"
                     /locus_tag="ACFB78_001936"
     CDS             316..1236
                     /gene="ribF"
                     /locus_tag="ACFB78_001936"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC1269861.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1378..4167
                     /gene="ileS"
                     /locus_tag="ACFB78_001937"
     CDS             1378..4167
                     /gene="ileS"
                     /locus_tag="ACFB78_001937"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC1269862.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1032 a         1222 c         1150 g          874 t
ORIGIN      
        1 acatagcacc gcgcctgctg cgcgttttat gcgtttggcg cgttcggcgg cgggaaattt
       61 gcctgctttt cccgcgtcgg gcgggcggaa cgggcggcac actgtctata aaccgcaata
      121 ccgtttacaa tgaccgcctg tttcaccaca tacccgaacg caacaatgtt tcaacacacg
      181 ggacggcaca tcaagcaccg ccctatgtgt cgtcctgatt tggaaggggt tgcacccctc
      241 ccgaataaag tctgatcctg ccgccccgaa ggacggatgt ccgagtggcg gggtttcaac
      301 cgaaaaggaa atacaatgaa aatcaggccg gggcggcaca acgcgcccga ctttccgcac
      361 ggggccgccg taaccatagg caatttcgac ggcgtgcacc tcggacacaa acacatcctc
      421 caaaaactcc gcctcgaagc cgacgcacgc ggattgcccg tcgttgccgt cattttcgag
      481 ccgcaaccca aagaattttt tgccgcccgc acaggcaaaa ccccgccgtg ccgtatcagc
      541 cccctgcgta ccaaactcgg gctgctcgaa aaaaccggtt gcgtcgatgc cgcctgggtt
      601 ttgcgtttcg atcggaattt ttccgaaata tccgcgcaag catttatcga ccgcctgctg
      661 cgtcaaacct tgaatacgcg ctatttgctc gtcggcgatg atttccgttt cggtgcgggg
      721 cgggaaggct gttttgaact tttggcacaa cagcccgata tgcaaaccga gcgcacgcct
      781 tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg tccgccaagc cctttcagac
      841 ggcaaccttg cctatgcgaa gaaacttttg gggcacgact acgttttggg gggcagggtg
      901 gtgcacggca gaaaactcgg acgcacctta aacgccccga ccgccaatat ccgcctgccc
      961 ggccaccgtt atgcactcgg cggcgtgttc gtcgtcgagg cggacggcgc attcggcacg
     1021 cggcgcggcg tggcgagctt cggcttcaat cccaccgttg atggcggctg ttctcaaaag
     1081 cttgaagtcc acctgttcga ctttcaaggc gatttgtacg gacaacggtt gaacgtccgc
     1141 ttcctgcaca aactgcgcga cgaggaaaag tttgacggta tggaagaact gaaaaggcgg
     1201 attgaagccg atatggaagc cgcaaagtgt tggtagaaaa accttataca aaccatccga
     1261 ttgggctaca atcagccttt taactgttca gacggcacag gggtttcccg ttgtgaaata
     1321 ctgtttgagg cgcaatgccg tctgaaaccg aaatattgta acaatagaga ttaaaaaatg
     1381 accgattaca gtaaaaccgt caacctgctc gaaagcccgt ttccgatgcg cggcaatctt
     1441 gccaagcgcg agcctgcgtg gctgaaaagc tggtacgagc aaaaacgtta ccaaaaactg
     1501 cgcgaaatcg ccaaaggccg tccgaaattc atcctgcacg acggcccgcc gtatgccaac
     1561 ggcgacatcc atatcggtca tgccgtcaat aaaatcctta aagacattat tatccgcagc
     1621 aaaacccaag ccggttttga cgcgccttat gtaccgggtt gggactgcca cggcctgccc
     1681 atcgaagtga tggtggaaaa actgcacggc aaagatatgc ctaaagcccg tttccgcgaa
     1741 ttgtgccgcg aatatgccgc cgaacagatt gcccgtcaga aaaaagactt tatccgcttg
     1801 ggcgtgttgg gcgattggga tcatccttac ctgactatgg atttcaaaac cgaagccgat
     1861 accgtgcgta tgctcggcga aatctacaaa tccggctatc tctaccgtgg tgcgaaaccg
     1921 gttcagtttt gcttggattg cggatcttcg ctggcggaag cggaagtgga atacaaagac
     1981 aaagtatcgc ctgcgattga tgttgcctat ccgtttaaag acaccgtcgc gcttgccgcc
     2041 gcattcggct tggcaggtat cgaaggcaaa gcgtttgccg tcatttggac gaccacgcct
     2101 tggactctgc ctgcgagcca ggccgtgtct gccggcgcgg acgtggtgta tcaattaatc
     2161 gatacgccca aaggcaaatt ggtgctggcg aaagatttgg cggaaggcgc tttgaaacgc
     2221 tacggctttt cagacggcat cgccatcctt gccgaaacca ccggcgacaa gctggaaaac
     2281 ctgcacatga atcatccgtt cctcgaacgc gatattccca tgctcaacgg cgaacacgtt
     2341 accaccgatg ccggtaccgg cttggtgcat actgcgcctg cgcacggttt ggaagactac
     2401 gccgtctgca ataaatacgg catcgagctt tacaaccctg tcaacgccga aggcaaatac
     2461 ataagcgaaa cgcctcgtgt cgcaggcatg agcgtttggg aggcgaatcc cgtcatcctg
     2521 caatggccgg aagaaaccgg caacctcttg gcaagcagca aaatcgaaca cagctacgcc
     2581 cactgctggc gccacaaaac cccgctgatt taccgagcga caggtcagtg gtttgtcggc
     2641 atggacaaag ccggcagcga cggtaaaacc ctgcgcgaca aagccatcaa agccgtggac
     2701 gacaccgaat tcttcccgcc atggggtcgt gcgcgtttgg aatccatgat tgaaggccgt
     2761 cctgactggg tggtttcacg ccaacgctat tggggcacgc cgatgacttt ctttgttcac
     2821 aaagaaacgg gcgagctgca tccgaactct gccgaacttt tggaaaaagt cgcgcaacgc
     2881 atcgaagaaa aaggcatcga ggcttggttc tccctcgata aaagcgaatt attaagcgcc
     2941 gaagattgcg aacattacga caaactcccc gataccatgg acgtatggtt cgactcaggc
     3001 tcgacgcatt attccgttgt aaaacaacgc gaagaattgg aatggccggc tgacttgtac
     3061 ctcgaaggca gcgaccaaca ccgcggctgg ttccaatcct ctatgctgac cggttgcgcc
     3121 tcatccatgg gacgcgcacc gtataaacag ctgctgaccc acggtttcgt ggttgaccaa
     3181 aacggccgca aaatgtcgaa atccatcggc aacgtcgtcg cgccgcagga agtgtataac
     3241 gagttcggcg cggacatcct gcgcctgtgg gcggcatcca ccgattacag cggcgaattg
     3301 gcgatttcca aagaaatcct caaacgcgta accgaaagct accgccgcat ccgcaatacc
     3361 ttgagcttcc tgtttgccaa cctcagcgac ttcaacccga ttgaagatgc cgtgcaacag
     3421 gcggatatgg tggaaatcga ccgctacgcc ttggtattgg cgcggcggct gcaagagcgt
     3481 ttggcaggcg gttactatcc gcgctatgcc ttccacttcg ccgtgaaaga cattgtttct
     3541 ttctgctcgg aagacttggg cgcgttctac ctcgacatcc tgaaagaccg cctctacacc
     3601 accaaagccg acagccgcgc ccgccgcagc gcgcaaaccg ccctgtacca catcacgcgc
     3661 agcctggttc tcttgattgc accgattttg tgcttcaccg gcgaagaagc gtgggacatc
     3721 atcggcggcg gcgaagaaga cagcgtcctc ttccatactt ggcacgagtt cccggccatc
     3781 aacgaaaaag ccgaagccga actggtgaaa aaatggacgg caatccgcga agcgcgcgaa
     3841 gcagtaaccg ccgccatcga gcctttgcgc gccgacaaaa ccgtcggttc gtccttgcag
     3901 gcggaagccg aaatcaccgc gccggaagaa atggccggct atctgaatgc tttgggcgaa
     3961 gaattgcgct ttgccctgct ggtgtctaaa gcagaagtga aagtcggtga tgaacttgcc
     4021 gttgccgcca aagccagcga cggcgaaaaa tgcgaacgct gctggcatta cacccgcgat
     4081 gtgggcgcgg ttgcaggtta tgaaaccgtc tgcaaacgct gtgcggagaa tgtcggcgga
     4141 gaaggcgaaa cgcgccatta cgcctgataa agtttgagca aatgccgtct gaaaccgcca
     4201 aacagtattt cagacggcat tttttgtgcc gcgatttgtc ttcataatgg cggaggggtt
     4261 tcgagattgc ggtgttgt
//
LOCUS       DAXDZG010000103         4219 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXDZG010000103 DAXDZG010000000
VERSION     DAXDZG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.103"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..228
                     /locus_tag="ACFB78_001938"
     CDS             <1..228
                     /locus_tag="ACFB78_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFC1269863.1"
                     /translation="KKTVKFQFPPEVVRVLAGMGNNLNQIAKALNTAAKVGTLGNVEA
                     LKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            213..845
                     /locus_tag="ACFB78_001939"
     CDS             213..845
                     /locus_tag="ACFB78_001939"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFC1269864.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQ
                     SMTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            938..1579
                     /locus_tag="ACFB78_001940"
     CDS             938..1579
                     /locus_tag="ACFB78_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFC1269865.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIRQRQSEILNQPAKR"
     gene            1626..1880
                     /locus_tag="ACFB78_001941"
     CDS             1626..1880
                     /locus_tag="ACFB78_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HFC1269866.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1858..2136)
                     /locus_tag="ACFB78_001942"
     CDS             complement(1858..2136)
                     /locus_tag="ACFB78_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFC1269867.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2136..2321)
                     /locus_tag="ACFB78_001943"
     CDS             complement(2136..2321)
                     /locus_tag="ACFB78_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFC1269868.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2324..2569)
                     /locus_tag="ACFB78_001944"
     CDS             complement(2324..2569)
                     /locus_tag="ACFB78_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269869.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2566..2808)
                     /locus_tag="ACFB78_001945"
     CDS             complement(2566..2808)
                     /locus_tag="ACFB78_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFC1269870.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2812..3681)
                     /locus_tag="ACFB78_001946"
     CDS             complement(2812..3681)
                     /locus_tag="ACFB78_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC1269871.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
BASE COUNT         1147 a         1043 c         1133 g          896 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactact
      601 acgccgccga cagaggaaga gtggacgcat ggcgcaccat gcagaacctg acgcacggat
      661 acagcgaccc agacgacccc gccaaacggc agagcatgac ccaagccaaa gacctgccga
      721 gaaacacgca ggaagccgca cagagcatca cagagcatca cagacggctt agaagcccta
      781 gccctatcag gcaagctaaa aagccgcgca gacgtgctgg aaacgctgga aaaggtaggt
      841 tttgaaatat cacgagcgac catcagcagc atcagcagca tcagcatcaa gaacccggac
      901 ccaaaagggc gcaacatccg actgaaaggc gcactgtatg agcaagattt ccgatttggc
      961 gaagaccttc gagcagacat cacgcaccga agccgccagc atagagcaac aaacgaaagc
     1021 agacttagag acgttacgga aaaatatcaa cgaggcattg aagcaaagcg agcagaaaat
     1081 aaccgccgat ataaacgccc ggcagttacg catgagcaag gcagtattca agccctatct
     1141 gtggagcttg ctaggtatat cggcggcagg gttgatagtc atagcagggc tgttcatagc
     1201 gatatggagc gtcaagaacg agctggacga cttgaaacag cagagagccg aagcagagcg
     1261 caccctagac ctgttggaaa ccaagaccaa aggtttgaca ctggaaaatt gcccagtcga
     1321 gaacagcaaa gcaacgcggg tatgcgtagc gaccgagaag cgaatgctgg acgcgttagc
     1381 ggaattagag agcaatcacg cagcaatcga gcagcgaatg atgaaagcct taacgcactt
     1441 gggcgaaagg ttggcagagc tagagcagga aaacacgagt ttagcgcagc agctagcgag
     1501 cttggcagcc gagttagagc ggcagagcga aatacggcaa cggcagagcg aaatcttgaa
     1561 tcaaccagcc aaacgataag ccaacgatac aaacgaaccc aaagcagggg atggggaatg
     1621 agccgatgat taccgagaac gaacgcgaca ggcgaacagc cgcatggctg atagagacct
     1681 acggggcaga agccgtagcg gaagcagaaa cccgcattgc gggcgcgaga aagccctatc
     1741 cgagcaatat cgccaaagta ttgggggcta gcctacccga agccctaaaa cgcacagaaa
     1801 acgccgcagc gcgccaaaaa ctggcagggc tgcggcggat gttggacggt aaggcagtta
     1861 agacttcaca agacttgtga aatcagacca ctgctcaatg cggaacgccc gaatatcgcg
     1921 gacagaagac ggaaaccaag gcagagcttt tagctcgttg atggctgaaa acaggttagc
     1981 catatcttcg ttttggcagg tgtacaaact tccctgaatg cgggtaaagc cgaatttccg
     2041 cagggtgtat ccaatatccg cgtaggcttg ggaaatgcct ttcggatggt tttgagcggt
     2101 atcggcaacc accaaatcaa aagaaatcgc gtacattagg cagcctttgg gtcttgaacg
     2161 atgtgggaca gtttgtattg cgtgtgttcg ccgctttccg gcagttcaat atttacgagc
     2221 caatcgccgt ccggaagctg ttcggcaggc gtgccgacga tgtacatcac gccgtaatcg
     2281 ccaaaggtgc ggattgaacc ggtggggata gtggtttgca tggttagttc tcctgttggg
     2341 tttggttttc aggctggttt gtcatgtaca aaacccgttg ggcagctttt tcggctgttg
     2401 tgcgtttgat gccgtcaggc agaaaaattc tccatttttt ggctagaact atatctgcaa
     2461 gatcatcaac cgttgcgctt ttcaaagcag gatttgcgtt aatttccgaa tacagaaaat
     2521 ctttgaatgc cgagtacaga gcatcgcggt ttattgcgta tcttgtcatg ctgtttcgct
     2581 ttggttgtca gtttcagacg gcataggctt gccgcgcctg taccaagtgg cgcgggaaat
     2641 gccgaagtgt tcccacggtt tatcgcggct gatagagttt tcggctaggt agtcggcgcg
     2701 ggattgcgac catgcccgaa aaacggttct tgtggaacag ccgaagcgtt ttgccagttc
     2761 tttagccgta acgtctcgct tggtggtttt cagttttgga taggccatat ttcaaagctc
     2821 cgtgatgatt tttgttctgc ctgcattgat ggttgttttc cgcaaactat tccgatgagc
     2881 ctgcaattcc gaaaaccgtc gattgctggc tgcaatatca aacttgtacc aaacccagtt
     2941 agcgattgat ttggagatac atttcacttc tttttcccac atcgggacag ggaaatcccc
     3001 atttacccgc atacactggt aatgcacttc tttcagccag ccttgcaccg tgtagccctg
     3061 ctgtttgaac gccaacacgt ttttgtgcgc ccaacggctc acaaggttaa acaccgtgca
     3121 atttctgctt aatccgaccg cttccacgtt agagcgaccg atatagggct taaacttgtc
     3181 taaatccacg aaatccgcaa gatactccaa atcgtagccc ctgattgcgt caggaacgcc
     3241 gcgcagcgtc agccaatgcg gatgttcggg atttttcgta atcagcgata caaagcccac
     3301 atcaccgcgc aatttcgcct tatatgctgc ttcaagtgca gcaagatagc gcagggcttt
     3361 ttgtctccca ccgtattccg ccgtcagcac aggcgcggaa agcgcatagg caaggtgtgc
     3421 gccgccgttt tccctgttga ttgccgccca agcaggcata ggcagattat tgtcttccca
     3481 agccaacccc gccccttcgt aatccaagtc aaagagcata aacacacgca gatgcggcgg
     3541 attgacttgg atgtagcggc gtttgatggc ggcagcgtaa gagcgcacca gcataggcgc
     3601 ttctttgaaa tctttgcagt atggcttgtg tgggatacgt tcttgcaaga agaggtcggg
     3661 ttgggtgtat aattggctca tgttgtatct cgaaaccccc gtgcagattg gcgtttggcg
     3721 ggggttttgc tttgtctaag atttgcagat tgtatgcttg tttttaagat gatacaacta
     3781 tgtcaaaata accataatca gataacagcc cgataggggt tcttatttca aaattttcca
     3841 atccgcaatt tagcgaagcc agcaggcgaa gcggtaaagc ttggagcgca gcagcgcgac
     3901 ctaagccggc cagcagggcg gcgttttggg ggaaacatga aaccagttcc gacagggcgg
     3961 cgtgcgtgtt cttcccggag ttcttcatgg agtatcggcg aaatgccgtg atgaaatgcc
     4021 gtttttttga gcagaaagca gtcaaaaaca ggggtatttt gcccttttga caggttcgag
     4081 tgccgccgaa aagcgaacaa agcaactcat catccgagtc agcccgaccg agtttgagac
     4141 tttgacccga cagaagaccc atccgaattt agcccgctac attcgggagc gggttttgga
     4201 agatggcaaa gcatccgac
//
LOCUS       DAXDZG010000104         3984 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXDZG010000104 DAXDZG010000000
VERSION     DAXDZG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.104"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            144..1225
                     /gene="hupA"
                     /locus_tag="ACFB78_001947"
                     /pseudo
     CDS             144..1225
                     /gene="hupA"
                     /locus_tag="ACFB78_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1253..3682
                     /gene="hupB"
                     /locus_tag="ACFB78_001948"
     CDS             1253..3682
                     /gene="hupB"
                     /locus_tag="ACFB78_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC1269872.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLTNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1070 a         1249 c          929 g          736 t
ORIGIN      
        1 ctataaaaaa caaaggtaga aacgcggcag gcaaacgcag gcacgtccga tttccgcgcc
       61 gccccataaa acagttgaca tcatataaca tttaaattga taatcaaaac tattattaat
      121 acatcatcaa gattgagtat gcgatgaaat acaaagccct gcccttactg ccgcttgccg
      181 ccgcccttgc cgcctgtgcc gggggggggt agccgaaccg cacgtccccg tgtccatccc
      241 caccgccacg ccgctgcccg ccggcgaggt aacgttatca agcgataacg gcaatatcga
      301 aaacatcaac accgccggcg ccggaagcgc atccgacgcg ccgagccgca gcagacgctc
      361 gctcgatgcc gccccgcaaa acacatccgg catttccatt cggcagcggg aagtagaaaa
      421 agactatttc ggctataaat caaaagaaac atcattcatc ttcaaaacac ccggcggcgc
      481 gcaatacgcc ctttcatcct acgccgaccc cataaccgtc agctactcct cccccgattt
      541 caaaattccc gaccgccacg cagggcagcg gctcgccgac ggcagccgca tctttatctg
      601 ctgcagcgac tccggggcta cttcgtatgc ggaaattacc aaacaagatt atatgaaatt
      661 cggggcgtgg ataggtccca acggcgaaat cgacctcttc gccggcggct tccccgtcgg
      721 caaaacgccg cctcctgcgt tctcctacgg cagcagcacg cccgaaaccg cattgtcaaa
      781 gggtaaaatc acttatcagg tttggggcat ccgcgtcaga aacgggcaat ttgttacttc
      841 ctcttatacg ccgccgaaaa gcggttctta ttatgggaca cttgccaata cccccgtcct
      901 ttcctttatc accgccaatt tcaacagcaa cacactggca ggcaaaatcc tcggcaacag
      961 cgattacggg ccggatgtgg atattcaaaa cgcaacgatt accggtccga ccttttccgg
     1021 cgatgccacg tccggcggga aaagcggcaa attggaaggc aagttttttg gcaagttcgc
     1081 cagcacccgc agcagcgaag tcagcatagg cggcaaaatc acctttgacg gcgaccgctc
     1141 cctcgatacc gtattcggcg gtgtgagtta tgaaaaaaaa cttgatgaca ccagtcaaga
     1201 taccaaccac cttactaaac aataaaccac ctcatccctg caaaggtttc ttatgcccat
     1261 tcccttcaaa cccgtattgg ctgccgccgc catcgcccaa gcgtttcccg cctttgcggc
     1321 agaccccgcg ccgcagtccg cccaaacgct gaacgaaatc accgttaccg gcacgcacaa
     1381 aacccaaaaa ctcggcgaag aaaaaatccg ccgcaaaacc ttagacaagc tcttgaccaa
     1441 cgacgaacac gacctggtgc gctacgaccc cggcatttcc gtcgtcgaag gcggcagggc
     1501 gggttctaac ggctttacca tacgcggcgt ggacaaagac cgcgtcgcca tcaacgttga
     1561 cgggctggcg caggcggaaa gccgctcttc cgaagccttc caagaattgt tcggcgcgta
     1621 cggcaacttc aacgccaacc gcaacacttc cgagccggaa aacttttccg aagtaaccat
     1681 caccaaaggc gcggactcgc tcaaatccgg cagcggcgca ttgggcggcg cagtcaatta
     1741 ccaaaccaaa tccgcaagcg actatgtttc cgaagacaag ccctaccatt tggggataaa
     1801 gggcggcagc gtcggcaaaa acagccaaaa attcagcagc attaccgccg ccggcaggct
     1861 ctttggtttg gatgccttat tggtttatac ccgccgcttc ggcaaagaaa ccaaaaaccg
     1921 ctcgaccgag ggcgatgtcg aaattaaaaa cgacggatat gtctttgacc cggccaaccc
     1981 ctcgcccagc agatacctta cctacaaagc cacaggggtt gctcgctccc aacccgaccc
     2041 gcaagaatgg gtaaacaaaa gcaccctgtt caagctgggc tacaatttca acgaccgcaa
     2101 ccgcatcggc tggatttttg aagactcgcg caccgaccgt tttaccaacg agctgtctaa
     2161 tttgtggacg ggtacgacca cgtctgccgc aacgggcgac taccgccacc gccaagacgt
     2221 gagctaccgc cgccgcaccg gcgtcgaata caaaaacgaa ttggaacacg gcccgtggga
     2281 cagcctcaag ctgcgctacg acaagcagcg catcgatatg aacacttgga cttgggacat
     2341 cccgaaaaat tacgatacaa acggcatcaa cggcgaggtt taccattcgt tccggcatat
     2401 ccgccaaaac accgcgcaat ggactgccga ttttgaaaaa caactcgact tttccaaagc
     2461 cgtttgggcg gcgcaatacg gcttaggcgg cggcagaggg gacaatgcca actcggatta
     2521 cagttatttc gcaaaactgt acgaccccaa aatcctcgct tccaaccaag ccaaaatcac
     2581 gatgctgatc gaaaaccggt cgaaatataa atttgcctat tggaacaatg tgtttcactt
     2641 gggcggcaac gaccgcttcc gcctgaatgc gggcatacgc tacgacaaaa acagcagcag
     2701 cgcgaaagac gatccgaaat acaccaccgc catccggggg cagattcccc atttgggttc
     2761 ggaacgcgcg cacgcgggct tcagctacgg cacagggttc gactggcggt ttaccaagca
     2821 tctgcacttg ttggcaaaat acagcaccgg cttccgcgca ccgacttcgg acgaaacctg
     2881 gctgctgttc ccacaccccg atttctacct gaaaaccaat cccgaactga aagcggaaaa
     2941 agccaaaaac tgggaattgg gcctggcggg cagcggcaaa gctggcagct tcaagctttc
     3001 gggcttcaaa accaaatacc gcgactttat cgaattgacg tatatgggcg tttcgtcaga
     3061 cgataaaaac aaccccagat acgccccgct ttcagacggc acggcattgg tcagctcgcc
     3121 cgtttggcaa aaccaaaacc gcaccgccgc ctgggtggaa ggcatagagt ttaacggcac
     3181 ttggaacctc gacagcatcg ggctgcccaa agggctgcac accggcctca acgtcagcta
     3241 catcaagggc aaggcaacgc aaaacaacgg caaagaaacg cccatcaacg cgctttcgcc
     3301 gtggacggcg gtttacagcc tgggctatga cgcgccttcc aaacgctggg gcgtcaacgc
     3361 ctacgccgcg cgcaccgccg ccaaaaagcc gtccgacacc gtccacagca acgacgactt
     3421 gaacaaaccg tggccttatg ccaaacacag caaggcctat acgctgttcg acctttccgc
     3481 ctacctcaac atcggcaaac aggttacgct ccgcgccgcc gcgtacaaca ttaccaacaa
     3541 gcagtactac acttgggaat ctttacgcag cgtccgcgag ttcggcacgg tcaaccgcgt
     3601 caacaacaaa acccacgccg gcatccaacg ctttacctcg ccgggcagga gctacaattt
     3661 caccatcgaa gcgaagttct aagctcctga tgtgccaatg ccgtctgaaa cccgatgatt
     3721 tgggtttcag acggcatttt ttacggtcgc accgccgccc cctttcaggc ttgtaccaag
     3781 cccaatcccg caactctccg tcattcccgc aactctccgt cattcccagg gaagtgggaa
     3841 tctagaaacg caaagccaca ggaacttatc cgaaacaaca gcaacctaaa attcgtcatt
     3901 cccacgaaag tgggaatcca gttcgttgag tttcagttat ttaaaataaa ttttgaaact
     3961 ttaatcccat cattcccgcg aaag
//
LOCUS       DAXDZG010000105         3922 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXDZG010000105 DAXDZG010000000
VERSION     DAXDZG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.105"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(75..509)
                     /gene="queD"
                     /locus_tag="ACFB78_001949"
     CDS             complement(75..509)
                     /gene="queD"
                     /locus_tag="ACFB78_001949"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC1269873.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(597..1112)
                     /locus_tag="ACFB78_001950"
     CDS             complement(597..1112)
                     /locus_tag="ACFB78_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC1269874.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(1140..1799)
                     /gene="queC"
                     /locus_tag="ACFB78_001951"
     CDS             complement(1140..1799)
                     /gene="queC"
                     /locus_tag="ACFB78_001951"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC1269875.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(1984..2189)
                     /locus_tag="ACFB78_001952"
                     /pseudo
     CDS             complement(1984..2189)
                     /locus_tag="ACFB78_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2288..3514
                     /locus_tag="ACFB78_001953"
     CDS             2288..3514
                     /locus_tag="ACFB78_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC1269876.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3535..3816)
                     /locus_tag="ACFB78_001954"
     CDS             complement(3535..3816)
                     /locus_tag="ACFB78_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1269877.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
BASE COUNT          781 a          991 c         1128 g         1022 t
ORIGIN      
        1 ctgaggcgag gcaacgccgt accggtttaa agttaatcca ccatacctta tttgaattga
       61 ctatatcgat acgttcaaaa tattccctcc cccccttcat actccgcgca cgatgtcggc
      121 gtttcccaca atttgacgcg gcacacgttc agccccgcgt ttttcagacg gcagtacatt
      181 tcgaccgcca tattttcggc agttgtgcgg cagggcaggc gcagggtttt catgttccag
      241 ccttccaaaa gcgcggcgat ttgagattcg cgcccgttgc cgccgtggta gatgaaggcg
      301 tggtcgaaat ggtcggtaat gtgttgtttc acaatggctt tcaagtcggt aaagtccatc
      361 accataccgt ctttcgctcc gcctttgatg gtgccgtctg aaacggtgat ttcgagtttg
      421 taggtatgtc cgtgcaggtt ttggcatttg ccgtcatgcc cgtcgagcat atgcgaggag
      481 tcgaaggtaa atatcttggt aattttcatc ccgattcctt ttcagtgaaa accccgccgc
      541 tttccgcccg gagggtggct tgatgtgtcg ttttgcgtgg aaacatatcc gctgcttcaa
      601 atgggcaaat agccttgaaa gccgatttcg gcgggtttgc cgtgcggatt cggggtcagg
      661 cggatatgga aattgccgat gtgctcgagc gcaagcaggt tgtccacgtc tttcaagttg
      721 tctttatgct gccgaacata gtcggtcaac agggtttgca gggctttttg tttggcttcg
      781 gcaggcgtgg cggcggcgaa cagcccgaac gcgtgcgcct cggcaagctg ccccgcgcga
      841 tagccgccga cattggcaaa atacaggcgc ggagcgtttt caggcggcat tttcgttttg
      901 ggcgtttcgg atacggcgat gtcgtaaccg tccgcccatt cgacaacctg ccaaccgtcg
      961 atgtggattt tgtccgtatc gccgaaccac gcggctttga gtgcggggac ggcttcgcgg
     1021 tagtcgtcgc acacggcaaa ttggatgtcg tgcacttcga tattcgaccg gccggcattg
     1081 ccgccgaggt aaaacatatg gagcttgggc atatcgtatt ccttgtgctt tgcgcgcgtt
     1141 cagacggcct ttttactttc cagacattcc gccagcccgc gttcgcgcaa gatacagctc
     1201 gggcattcgc ggcagccgcc gacgataccc ttgtagcagg tgtgggtctg ttcgcggatg
     1261 tagtccaaca cgcccatttc gtccgccaac gcccacgttt gcgccttggt caggtacatc
     1321 agcggcgtgt ggatttgaaa atcgtagtcc atcgccagat taagggtaac gttcatggat
     1381 ttgacgaaca cgccgcggca gtcgggatag cccgaaaaat cggtttcgca cacgcccgcg
     1441 atgatgtgcc ggataccctg ccctttggca aaaatggcgg cgtaaagcag gaaaagcgcg
     1501 ttacgcccgt ccacaaaggt attgggaacg ccgttgtcgg cggtttcgat ggcggcggtt
     1561 tcgtccatca gggcgttgtg cgtaatctgc cgcatcaggc tcaaatcgag tacggtttga
     1621 ctgacaccca aatcctgcgc gatccactct gcgcgttcca gctcgacggc atggcgttgc
     1681 ccgtatcgga aggtgatggc ttggacgttt tcgcgcccgt aggtttggat tgcctgaatc
     1741 aggcaggtgg tcgaatcctg accgcccgag aagatgacca aggctttttg gtttgacata
     1801 tcgaatccta gttttggtaa cgcgggaggt ttgcgaaccg cgaagttgga cagtaaagac
     1861 gcggattata caggcaaacg cgttcagacg gcatctgaac gccatgccgt ctgaacacgc
     1921 ggcgtatgcc gacttgacgg cgagttccgc caagcgtctg ccttgtgcga aggcgatatt
     1981 gttttcttcg gcggtcagga cgggagccgc tgtcgggaat atctttattt gacggcttcg
     2041 cagacggcgg acactttggg gacggtgcgc aatacggctt cgcaaccttc aacgctgtcg
     2101 atgccgcgag tgattcggcg tgcgggattg cgggtgctgc cgttttggga atagtagagg
     2161 acgaggattt tgaggggatt tgggttcatg ggcggattcc gttaaaatag tttggtgttc
     2221 agacggcata acggttgtcc agatgccgtc tgaaacagtt tgaaaacatg aaacctggta
     2281 agagattatg acctttttac aacgttggca aggtttggcg gacaataaaa tctgtgcatt
     2341 tgcatggttc gtcatccgcc gtttcagtga agagcgcgta ccgcaggcag cggcgagcat
     2401 gacgtttacg acactgctgg cactcgtccc cgtactgacc gtaatggtcg cggtcgcttc
     2461 gattttcccc gtgttcgacc gctggtcgga ttcgttcgtc tccttcgtca accaaaccat
     2521 tgtgccgcag ggcgcggata tggtgttcga ctatatcgac gcattccgcg atcaggcaaa
     2581 ccggctgacc gccatcggca gcgtgatgct ggtcgtaacc tcgctgatgc tgattcggac
     2641 gatagacaat gcgttcaacc gcatctggcg ggttaacacg caacgcccct ggatgatgca
     2701 gttcctcgtt tattgggcgt tgctgacttt cgggcctttg tctttgggtg tgggcatttc
     2761 ctttatggtc gggtcggttc aagactccgt actctcctcc ggagcgcaac aatgggcgga
     2821 cgcgttgaag acggcggcaa ggctggcttt catgacgctt ttgctgtggg ggctgtaccg
     2881 cttcgtgccc aaccgcttcg tgcccgcccg gcaggcgttt gtcggagctt tgattacggc
     2941 attctgcctg gagacggcac gtttcctgtt cacctggtat atgggcaatt tcgacggcta
     3001 ccgctcgatt tacggcgcgt ttgccgccgt gccgtttttc ctgctgtggt taaacctgct
     3061 gtggacgctg gtcttgggcg gggcggtgct gacttcgtcg ctgtcttatt ggcagggcga
     3121 ggccttccgc aggggattcg actcgcgcgg acggtttgac gacgtgttga aaatcctgct
     3181 gcttctggat gcggcgcaaa aagaaggccg aaccctgtcc gttcaggagt tcagacggca
     3241 tatcaatatg ggttacgatg aattgggcga gcttttggaa aagctggcgc ggtacggcta
     3301 tatctattcc ggcagacagg gctgggtttt gaaaacgggg gcggattcga ttgagttgag
     3361 cgaactcttc aagctcttcg tgtaccgccc gttgcctgtg gaaagggatc atgtgaacca
     3421 agctgtcgat gcggtaatga cgccgtgttt gcagactttg aacatgacgc tggcggagtt
     3481 tgacgctcag gcgaaaaaac agcagcagtc ttgagtattt ttgaaactgt atttttatcc
     3541 taaagacatt ttcctatctg ctgattccac cgtttccttt ctgtgtgccg ccataatttt
     3601 tataatagac aagccattca agtttgcatt gactgctttt tcattggcaa tatccaaatg
     3661 actggccgct tcatctaaaa ataggatttt cggttcgcaa tataaggccc gcgccaatac
     3721 gatacgttgt ttttgtccgc ctgacagtgc gctgcccata tcgccgatca aggtttcata
     3781 gcccataggc atggcggaga tttcttcgcg aatcatagca aggcaggcgc aatgttcgat
     3841 ttttcctcta tccggttctg tatcgaaaaa acagatattc tctgcaattg agccagcaaa
     3901 tgcaaatata gtggattaaa tt
//
LOCUS       DAXDZG010000106         3746 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXDZG010000106 DAXDZG010000000
VERSION     DAXDZG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.106"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            247..1008
                     /locus_tag="ACFB78_001955"
     CDS             247..1008
                     /locus_tag="ACFB78_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269878.1"
                     /translation="MEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYESMCGSAG
                     SHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLMGKGKEPA
                     GVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQNQARRIL
                     GERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKREMFAEPY
                     PFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(1107..1508)
                     /locus_tag="ACFB78_001956"
     CDS             complement(1107..1508)
                     /locus_tag="ACFB78_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC1269879.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1610..1792)
                     /locus_tag="ACFB78_001957"
     CDS             complement(1610..1792)
                     /locus_tag="ACFB78_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC1269880.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1962..2780)
                     /locus_tag="ACFB78_001958"
     CDS             complement(1962..2780)
                     /locus_tag="ACFB78_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC1269881.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVAHSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2777..3496)
                     /locus_tag="ACFB78_001959"
     CDS             complement(2777..3496)
                     /locus_tag="ACFB78_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HFC1269882.1"
                     /translation="MLQIQTIMDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINE
                     WIGGNMAQALGLPVAPFELLEVGEELYEELPAKMKEIGKGICFGSLAQKGCALLEPAD
                     IPRIDTVMQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNF
                     LQNHIFSSAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAY
                     NRSRTDETVRRIDNGTLWSIS"
BASE COUNT          891 a          859 c          974 g         1022 t
ORIGIN      
        1 gccaaaccga tttattctgc cggttaattc gttcatcccg tttccttcaa gttgttgttt
       61 gtttcgatgg gtgtatagta ctaaagtact tatatattgt aaagtacaat agtactatta
      121 ttttatattt agtatattag tatttgattt tcaaaagaat ttatttttga gattccgcag
      181 gcgcaaaaaa accgcccgat atttcgggcg gcgtattaaa atcagatgaa attaaggagg
      241 cttattatgg aaatgcaatt ggacaaacag acattgacag tggttgtcag aaacagcgag
      301 ccggtggaat tgtcggtatt tgcgcagtct atgatgagcc ttgccgatga ttacgaatcc
      361 atgtgcggca gcgcgggcag tcatgcgcgg ctgtatatta aggaaatcag gcagggcagc
      421 attattgccg agcttgcacc cctcttgcct ttggcgggta cgctttttga aggcgcgggg
      481 cagatattgg cgtacgccaa gaattttatt gagattaccg attggctgat gggtaaaggc
      541 aaagaacccg caggggtaac ggacagccaa atccgcaata ttgcaaatat catgcagcct
      601 gccgcatcgg ataaaaacgg gtcgattgaa atcaacgtga acgacaacaa cggctccgtc
      661 gtcaataata ttacctacaa ctatttcgcg gcaaacacgg tacaaaatca ggcaaggcgg
      721 atactgggcg aacgggcgga agcgtccgaa tcaggggatt acgggcagat ggtgatgtat
      781 ttcgtacaag ccgcgccgac aaaggaaacc aatcaggcgg ttattgaagg gatatacagc
      841 cgtccggtca aaatcctgat tcccgaacac atcaagcgcg aaatgtttgc agagccttat
      901 ccatttgaaa aatattatat tgttgacgta agcgtacaga cggcgcgcgg caagcccagg
      961 ctctacaaag ttaccgggta tcacggggta gtcgatgggg acgattgaaa accgcctttt
     1021 cgggcggctt tgtcggtttt gtgttgtttt caggttcggc ggggcgtgaa aaaagcccgc
     1081 gtgtgcgggc gggttgaacg ggctggctat gcctgattca tggtaagcag ggcggctttg
     1141 gctaaaaaac cgctacgggt ttcatgattt gcggatgtat attcatccac tctatcaaga
     1201 aggtattgcg gccagctgac gttgaatcgg atttgctgcc gactgatttt tgcagggtcg
     1261 atttcaatca ttacccatgt tgccccatga taatcaggtt cttggctcaa atcggcaatg
     1321 ctgcttacgg caaggttttt gaaccctccg tcctcaatca taccgtcaat atgcatatag
     1381 gcggcagaac gggcgttggc gacggcttct tcaacggtgt cgccgcaaga gaaacagccg
     1441 ggaaggtcgg ggatggttac gccatatgcc gaatgttcgt ctttgtgcag ggcggcaggg
     1501 ataaacattt gatttccttt aattatgttg ttcaggttta tgggcgcgtg agataacccg
     1561 tcgattgttt gattagaagg ggggcggcgg gaaaccccgc tgcctgccgt tacttcaaac
     1621 cggcttgctt atagatattt tttacagtac ctgtcggcaa gtcttttttc gggtgcggca
     1681 cggttacacg gccttttttt gttgggtgct tatattgcga atggctcccg gattgtgcaa
     1741 ctttatacca accgtcttgt ttgagtaggg caataacgtc taggctattc aattatcact
     1801 cctaatctta atgcatggca cgcctgccaa tgtgcgtaat aatacacact aatttttatt
     1861 ttgtcaattt ttatgtgtat ttgtgtgtaa aaatatcttt cggcagtagt tgcagggaag
     1921 ggtgtaaaaa gcccgcataa cgcgggcggt cttgagggct gtcagccgca ggcaaagttt
     1981 ctgatgattt tgccttcgct tcggttatcg gcaagccgta tgccctgttc ctccaggtcg
     2041 cccaatacgt caagcaatgc cttttcttgt gccttgtcgg gacgctccgg cggttcgtag
     2101 gcaaacaata tttcagagcg gtcgatgtat ccgccgcgcc gcaaccgttt tatgccggca
     2161 atccacttat cagacttgta atagatgtcg gcagggtctt tctgtccgaa atagataggc
     2221 ttgatgattt tccgtatttc gccgttttcc gccttttgca ccaaggggat ggaggcgtga
     2281 aagcctgacg ggtcgccgat tgtgctttct ttgaaggggt atgccgtctg aagcggcttt
     2341 aacattgcct gtatttgtcg ggtaagttct gcttcgggtt ggcttttagc aaagctgtgg
     2401 gcgacaaaat aatcaaacag gcggttcagt tcctgccccc tgtcgggggc gagggttacg
     2461 cccggttggg cggccataat cagggcttcg cgcgggcggg tcaaatggtc gagcatggcg
     2521 cgtatctgat cgggcgcgga gtatgccgcc aatttcccaa tccgctgtaa ttcttctgca
     2581 aaggcacggg tcgccgcttt atagacggac gaatcgaagc ggcggaaaaa acggctcaat
     2641 ctgctgtaac gctgttcgat tttaaagtcg aagtagccgc ttttgggatg ggttatgatg
     2701 atgccgatgt tggcaaattc gcgcgtttgg acatagggca taaaccgtat gacggcaaaa
     2761 cgcatggcat attggttcat gagatgctcc aaagtgttcc gttatcaatg cggcggacgg
     2821 tttcgtcggt gcggctgcgg ttataggcgg cgggcaggtc gcgctcttcg tttgcccatg
     2881 cccactccgg cggcagatta tcacacgctt ttctgtaggc tggcaatgcg cttttcagcc
     2941 ataattccat ctcttcccga agcacccaat cttccaaaat ctgtctaaat gccgatgaga
     3001 aaatatgatt ttgcagaaag ttatttggat tgaagccggt gtcgaaggcg cagttatgat
     3061 caataacaat caaggaattg ttgcaatttc tatacaaaag gttagggttg ccgattgttc
     3121 tgtcttcatt acggacaaac caatcgaaag cggcgatttg ccgctgcatg actgtatcga
     3181 ttcgcgggat gtccgcaggc tccagtaacg cacagccttt ttgcgccagt gagccgaaac
     3241 agatgccttt gccgatttct ttcatctttg cgggcaattc ttcgtacagc tcttctccga
     3301 cttccaacag ctcaaacgga gcaacgggca agcccaaagc ctgcgccatg tttccgccta
     3361 tccattcgtt tatttggctt gcacgggtgg cgtgcagccc tttaacgaaa tattccagac
     3421 cattctccgc catacagatg aacggcgagg taacaccttg ctccgcacga tccattattg
     3481 tctgtatctg caacatttct tttctttatc ctatgtttca acacacagga cgacacataa
     3541 agcaccgccc tatgtgttgc cctgattcgg aaggggttac gcccctccca aacagagtct
     3601 gatcctgccg ccctaaaggg cggggtttca accgaaaagg aaatacgatg aaatttacac
     3661 tttgtgtaaa tctgtaaatc cgactttttg agatttcccc ctagaggaat ttgtcaatcc
     3721 agcacgctcc gccaaaatac cctacc
//
LOCUS       DAXDZG010000107         3684 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXDZG010000107 DAXDZG010000000
VERSION     DAXDZG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.107"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..519
                     /locus_tag="ACFB78_001960"
     CDS             <1..519
                     /locus_tag="ACFB78_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1269883.1"
                     /translation="VASNVSLDMSNPTVLRMGLPLYIASLRRGAIYKVWQFVEDALRA
                     VVPADSFEPTAQKLKLFKAGAATILFYEDQNVVKGLQEQFLAYAANFPVWADQANAMV
                     QYAVWTTLAAVGAGANLQHYNPLPDVAIAKAWNIPENWLLRAQMVIGGIEGAAGEKVF
                     EPVAERLKVFGA"
     gene            complement(574..1029)
                     /locus_tag="ACFB78_001961"
     CDS             complement(574..1029)
                     /locus_tag="ACFB78_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC1269884.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1285..2646)
                     /gene="ffh"
                     /locus_tag="ACFB78_001962"
     CDS             complement(1285..2646)
                     /gene="ffh"
                     /locus_tag="ACFB78_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC1269885.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMF
                     PGL"
     gene            2866..3672
                     /locus_tag="ACFB78_001963"
     CDS             2866..3672
                     /locus_tag="ACFB78_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC1269886.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          808 a          909 c          990 g          977 t
ORIGIN      
        1 gttgcgtcaa atgtcagctt ggatatgtcc aatcctacgg tgttacgcat gggattaccc
       61 ttatatattg cgtccctaag aaggggcgca atatataagg tgtggcaatt tgtcgaagac
      121 gcgctgcgtg ccgtcgtgcc tgccgacagt tttgaaccga ccgcgcaaaa attgaagctg
      181 tttaaggcgg gcgcggcaac cattttgttt tatgaagatc aaaatgtcgt caaaggtttg
      241 caggagcagt tccttgctta tgccgccaac tttcccgttt gggcggacca ggcgaacgct
      301 atggtacagt atgccgtctg gacgacactt gccgcggtcg gtgcaggtgc aaatctgcaa
      361 cattacaacc ccttgcccga tgtggcgatt gctaaagcgt ggaatattcc cgaaaactgg
      421 ctgttgcgcg cgcaaatggt tatcggcggt attgaagggg cggcaggtga aaaggtcttt
      481 gaacccgttg cggaacgttt gaaagtgttc ggcgcataat ttcgcggtca aaaaaatgcc
      541 gtctgaaccc cgttcagacg gcatttttca gtatcaggcg gcgagttttt cgcattcttt
      601 gatacagtcg aggcaggact cgtaacaggc tttgcattcc gcatggtggg cggagtgttc
      661 tttacaggct ttggcacact gtttgcacgc ttcgaggcac acttttgccg cgtcgcgtgt
      721 cagaggggaa ttttgtgcgg caaggtcgtg caccgcgccg cataaggcaa gcatctggcg
      781 cacggcaacc gcacagtcgg acatagacgt gtcgccctga gtgagcaggg acaggcagtg
      841 ggtcaggcaa acctgtccgg catccagaca gtgtgcggca gtttggcgga cggcggtgta
      901 ggcggatgcg gcggcaggct gcatatcgtg atgatggtgg tagtcggcgt gtccgtgcgc
      961 acgcgcgaaa gaggcggcgg aagccaaaga gacggcagcg gcgctgccca aaaattgacg
     1021 acggttcatg attttttcct ttatgtttca acatgcgggt ccggtataag gtacacactt
     1081 accatactgc cctggccgga tcggtacgat tttctgaata attgcttgat tttgcaggtg
     1141 tgaaattgtc gcacgagcag gcatgtttta gctattcgga aattcgagga agggtgcatc
     1201 aacctgtttt gaaatgatgc gcagacatgg taacagacaa aaagccgtct gaaaatcgtt
     1261 ttagacggct tttgttttca aacattaaag accgggaaac attcccttca tgcctttggc
     1321 caagcgcatc agtttgccca aaccgttgcc gctgaacatc ttcatcatct gctgcatttg
     1381 ttcaaactgt ttgagcaatt tgttcacttc ctgcacggtc gtgcccgcgc ccatcgcaat
     1441 acggcgtttg cggccggctt tgagcagggc agggttggcg cgttctttag gggtcatcga
     1501 gttgatgatg gcttctactt tgcccatcgc tttttcagcc gtaccttcgg ggatttgttt
     1561 cgatatttga cccagttcgc ccggcatttt cgacatcagg ttttccaaac cgcccatatt
     1621 gcgcatttgc tggatttgtt ctttaaaatc attgaggtca aagcctttgc ctttttgcag
     1681 ctttttcgcc attttggcgg cggcttcttc atcaatacct ttttgaacgt cttcaatcag
     1741 ggtcaatacg tcgcccatgc cgaggatacg gctggcaaga cggtcggggt ggaaaggttc
     1801 gaggccgttg attttctcgc cgacgccgat aaatttaatc ggtttgccgg ttacatggcg
     1861 tacggacaat gccgcaccgc cgcgcgagtc gccgtccatc ttggtcaata cgacgccgtt
     1921 cagcggcagg gcttcattaa atgcctgagc ggtatttacc gcatcctgac ccagcatggc
     1981 atcgataacg aacaaagttt caaccgggtt gaccaccgca tgaagcgctt tgatttcgtt
     2041 catcatctct tcatcgattg ccaaacggcc ggcggtatcg accatcaata catcgtaaaa
     2101 atgttttttg gcgtaatcaa cggcggcagt tgcaatctca accggttttt ggttggtatc
     2161 ggatgggaaa aaatctacgc cgacctgttc ggccaacagg cgcagctgtt caatcgcggc
     2221 agggcggtaa acgtcggcgg ataccaccaa aacttttttc ttctgatcgt ttttcagcag
     2281 gcgggcgagt ttgccgacgg ttgttgtctt accggcaccc tgcaaaccgg ccatcaaaat
     2341 gatggcaggc ggtgaaaccg ataaatccag tgttttgttt tccttgccca tcagttcgac
     2401 taaggcttgg ttgaccacgc cgataaaggc ttggtcaggc gtcaggctgc ccgccacttc
     2461 ctgaccgagg gccttttctt tgacgttgtt gacgaactct ttgacgacag gcagggcgac
     2521 atccgcctca agcagggcga gtcggacttc gcgcaaggct tctttaatat tgtcttcggt
     2581 cagtttggcc tgcccccgga tgtttttgaa gacattgctg aagcggccgg ttaaattgtc
     2641 taacatactg gtccttggtc tgaataagaa tagcttgccc catcaggggc attctttgtt
     2701 aaaataaaat caaaataatt tgatgcggct tgtgtgccgg acagcatatc ggcaaatccg
     2761 tcaaggcttg cccgaaacgg ggattttaca attccaacgt taaaagttcc aatatttcat
     2821 aagcggccgc atacggcgca acagtataga tagagaaagt ccaccatgcc gacagttttc
     2881 atctttttga cggcggttta cgcaggattg ggtgcatttg catggcactg ccaacagcag
     2941 gggcgcggcc gggattaccc gtggaagacg gaattgccgg ttttgggcgc ggcattgacc
     3001 gtccacggcg cggcactgct gatgccggtc attcaagaca aaatcatcat tatgggcttc
     3061 gggtattccg gcagcctgat tgtttggatg atgctgttta tttattttgc cggcagcttt
     3121 ttttatccgc tgcgcggagt gcagttgctg ctgtatcctt gcgccgcact gatgctgctg
     3181 tcaggtttgg ttttcccggg aaaattctcg ggatatgaaa ttaccgactt tccctttatg
     3241 ctgcatatcg gcacatcact gcttgcatac gggctgttcg gcatcgcaac attattgtcc
     3301 gttttgagct tgctgctgca ccggagcctg caccgcagaa acttctccaa gctcgcagga
     3361 ttcctgccgt cgctgctcag tttggaaaaa ctcatgttcc aggccatgtg ggcaggtttc
     3421 atcctgctga cctattccgt cgtcagcggc acattctttg ccgaagccgt attcggcaaa
     3481 cccatgacat ttacccataa aaccgtattc ggcatattgt catggctgat ttacggagga
     3541 ctgctgctca aacacagcat gaccgcatgg cgcggcaaaa aagccgccgt gtggaccatc
     3601 atcggatttg tcagccttat gattgcctat atgggcagca agttcgtatt ggaaatcatt
     3661 ctgaaaagat aagaagagcc aaca
//
LOCUS       DAXDZG010000108         3629 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXDZG010000108 DAXDZG010000000
VERSION     DAXDZG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.108"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..299)
                     /locus_tag="ACFB78_001964"
     CDS             complement(<1..299)
                     /locus_tag="ACFB78_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC1269887.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALALVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQHLTVRHVSTGASSPGKVSTVVSSS"
     gene            complement(646..948)
                     /locus_tag="ACFB78_001965"
     CDS             complement(646..948)
                     /locus_tag="ACFB78_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269888.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLMINFLKDIGKVGTD"
     gene            complement(976..1206)
                     /locus_tag="ACFB78_001966"
     CDS             complement(976..1206)
                     /locus_tag="ACFB78_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269889.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDAAAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1433..1633)
                     /locus_tag="ACFB78_001967"
     CDS             complement(1433..1633)
                     /locus_tag="ACFB78_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269890.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            complement(1637..1948)
                     /locus_tag="ACFB78_001968"
     CDS             complement(1637..1948)
                     /locus_tag="ACFB78_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269891.1"
                     /translation="MFNQNQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSRNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1960..3174)
                     /locus_tag="ACFB78_001969"
     CDS             complement(1960..3174)
                     /locus_tag="ACFB78_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC1269892.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFIDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSGKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFIDDSIRPRITRIDLALDFFNGEYTP
                     DQALLDHDNGFFDNSNQRPKSEMIGTAWRNEDGTGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPFPERFNQRNKT
                     LNLTFEHKLRYAKNAVGKLVNFMIEIGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDAEKERKYQDYLD
                     EVYHQNVNYDYF"
BASE COUNT         1149 a          822 c          557 g         1101 t
ORIGIN      
        1 gatgaagata caaccgtact aactttacca ggacttgatg ccccagtaga tacatgccta
       61 actgttaaat gttgagcttc tgaagttaaa tatcttcccg attctctatc aaatacacct
      121 ttccaaattc ttcttctttt gtcatctgaa cctcgaatac ctaaatatct gccatcatca
      181 taaacttcaa ccctatcaaa tttacccgga acatttacta atgccaatgc aaatttaggt
      241 acaaaaaaac taaagcatag gataatcttt aaaaaatttc gccctaggat aagaaacata
      301 acattttgct tcatctaatt taattaataa tgtagaagaa tcagggaatt gaatttctat
      361 aaaatctcta tcataacgat gaaagaaatt cctaaaaata tcaaatgaaa acgaagattt
      421 aaaaacccct tttctaatca attccctata ataaattaac gcttgtggta aatctctata
      481 aatatgtgaa tcagtttgaa atttaactaa atgatattca gaaataaaca taattcggac
      541 cttttaaaac actctaaaaa gccgattcta ccaaccgtta ccaaaacagc caagcaacag
      601 acagagccaa gaagaaacca agaagaaacc agaaatcaat aaccatcaat cagtcccaac
      661 cttgcctatg tcttttaaaa aattaatcat cagcctaaag ccgtaaatga ctacgaacag
      721 aattagaacc gtcgaaccga cataagaacc ttgctttatc tgctcaaaat tggaacattc
      781 cggataagac aacgtaaccg gctttccgtt caaaatccat ttatcgccca ccctttccgg
      841 cctgatgatt tttccgtcct gggtaacagt aggaggaagg gacgacaata aatagtcgtc
      901 tgcctgcaat cttgtatcaa aacaatttat gccgacacga tagcccattc atgcgcccct
      961 ttttcttcac tctgtttatt tgacagattt aatcatgctc caagccattt tgaagccttg
     1021 gattgcaaga atcacggtaa tggccgccat acccacggcg gaaaccattg acacgaaacc
     1081 catgattaca ttcgctactt gcgtaccaat tgcggctgca tcaaaggtat ctgccataac
     1141 aatggccggt gtgaagatac cggctgccaa ggctgctttt acagcgtatt ttttaacgat
     1201 gttcatcgtt tttttccttt tttgatattt aaagtaagac ggcttcttag gtttaaatcc
     1261 gggagaagcc tgctcccgaa ttttgttttt aatttatgaa atagagaatt gagaaaatga
     1321 aaagaatgag gcaaacaaaa aatccgataa ttaaagttgc tttattcatt tttaatcctt
     1381 tttgcgggct ttgtgaaagg ttgacagacc gcccgccgag cctgttttac ttttattccg
     1441 atttcacgaa gaactgaaat atttggaatc ctccgcctat ttcatttatg cctgagttca
     1501 atgcatcttc gtaactttca aattgacctg ctgatttaat attttgagta aaccccacgt
     1561 caccgaacgg atcgggataa ataaagtcat gcgtttctaa atcttgaact atgaaacgtt
     1621 cttcaaattt cataaatcaa cctttcggtt tttccgccac ctgaaaatca attaatgaag
     1681 gaaccatgcc tttacctgtc gaagtcattt caaccgttac cataacttcg cacgggtatt
     1741 tgagattctc taattttgag aaattcctac tgtccccgaa tttcatttgt gccgccgtga
     1801 atccgacagc attccccgac tgtgccggta aaggtgttgc aaccaacaca gaacaagtat
     1861 ctatattaga gccgtcaatt tcgcctttga attttttagc tcccaaaaaa gtcgcaggat
     1921 aagttacagt ttggttttga ttaaacattt tgatttcctt taaaaataat cataatttac
     1981 attttgatga taaacttcat ctagataatc ctgatatttc ctttctttct cggcatcata
     2041 atcaggacta aaaagccttt tttccctatc gaatttgtca gaatttttaa aagcaataac
     2101 ccccaattca tcaagttcaa tttccaaatc aatatccggc tgttcatgaa taaaaccgtg
     2161 ttttaacccg tcccttaaca tttccagagc atatttttca ggttctaacc ctttaggaaa
     2221 tcccgaatct gcctttaaag attccacaat ttcactatta tcaaaaccta tttcaatcat
     2281 gaaattgacc agttttccaa ccgcgttttt tgcataacgc aatttatgct caaaagttag
     2341 attaagcgtt ttatttctct gattaaacct ttcgggaaac ggcatatttt taaattttct
     2401 acaaattgga aaagctccac agaaatacga accctgattt attaaaatat ccaagggtat
     2461 ttcaatatct ccataattaa actggatttc gaaccttacc cacttgcttt ctttatcgcc
     2521 aagctgcctg cctttctcat aaacacgaac aaaacgagaa tttttcttgc gacctacata
     2581 aaatgtcttt ccggtcccgt cctcattccg ccaagccgtc ccgatcattt cagatttcgg
     2641 cctttggtta ctgttgtcga aaaacccatt atcgtgatct aacaatgcct gatccggcgt
     2701 gtactctccg ttaaaaaaat caagtgctag gtcaattcgc gttattcttg gccttatcga
     2761 atcatcaata aactgcttta acctcaactc ccaacccgga cttgcaacgc tgcaaccagt
     2821 acctttcaac tcaactagaa cagtattccg ctgtcctccg aaatgcacct ctccataatc
     2881 aacatcatcc gaacctagcc tatacatgga ttcatagaat ttgttgcccc ttgatttgca
     2941 tttgcgcgtt atgccaaaac ctagaatttc ttccagcttt ccgcttaata catacatgta
     3001 ttcagcatca gaaaataaag gacacccgga aactttcagt aaagtatctt catggaatgt
     3061 gaatgaaatc caatcaataa aaacaccgtc ttgcttacct cttcgctgcg gaatttctaa
     3121 aagctttccc tttccgtccg atataaagtg tgagaaatat tcaacttcac tcattcgttc
     3181 actactcttg gaatttggtt tttaatttcg ctccccccct gttagtcaag gggggggctt
     3241 tcagccgttt cccgtcttcc gcgctaaagc gcgtacgacg gtcaacggct gaaagccaaa
     3301 tcctgacaaa ctgttaaaga tcaacaaaaa agaccacaac catccgttgt gataatcact
     3361 ggaaaattcg aaccaaccga atctatataa tcgaacgcct gataaagctt tgaaaaattc
     3421 tcttgattaa tgagtttatg cggttcgcca tgtctgaact gataaaaaca caaaacacaa
     3481 gaatctgatt ttttaaatat tctccagtaa gaacaggaga atattacatt tgctgctgac
     3541 ataaaaaagc ccctttcact tggctgtcaa aggggaatgt taagaaaagt aatgcgcccc
     3601 tttgatagag cgcatcatat aaggcggga
//
LOCUS       DAXDZG010000109         3490 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXDZG010000109 DAXDZG010000000
VERSION     DAXDZG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.109"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(308..523)
                     /locus_tag="ACFB78_001970"
     CDS             complement(308..523)
                     /locus_tag="ACFB78_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269893.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACFB78_001971"
     CDS             complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACFB78_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC1269894.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDVFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNSNSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEEHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTRIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSESEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          810 a          862 c          857 g          961 t
ORIGIN      
        1 agtaaataaa aaataacaac ataaatcaat ttattacggt tttattttta tttggtgttg
       61 acgggcaaca tatcatctgc gcggtaatga tgagatttta ggttgtggga tttatcggaa
      121 aaacagaaac ctctccgccg ccattctcac gaaagtggga atccggaaag ctaaagccac
      181 aggaatttat cggaaatggc tgaaatttat agtggattaa caaaaatcag gacaaggcga
      241 cgaagctgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac ctgtttttgt
      301 taatccacta taaaaaaact gattcccgct tttgcgggaa tgacgactca taaattaccc
      361 gaaacaacca aaaataaccg aaatcaaaaa ccggcttccc tgcgtgggaa tgaaatggat
      421 ttttctttga gttgccgatg cgataaaacg gattggcata ggcttgcggc aggatgatgc
      481 gtttggggaa acaaatgccg tctgaagggc tttcagacgg cattgcagtt tttccacaaa
      541 acgcgggctg cctttaaaac ttgtagctca tcgtcatcaa gaaggtgcgt ccgcgtgcga
      601 aattggtcaa tacgcttttg cttgtgccgc cgtatttgcc gttgcacaac gttttatcag
      661 cattacacgt tacgtcttcg tccttgtctt tcgggtcgaa cgagctgtaa taacgctgcg
      721 ttgccgcatc attgcccgca tcgagcggat cgatataacg cctgtcgaac aggtttttga
      781 cttcggcgcg gaaaataagg tttttcttcg gctcgtaagc ggcgtaaaaa tcaaaaatca
      841 aaggctgtcg ggcaagggtt tcggtttgtt tgatggaacg cttgcccagt tgccggacat
      901 tgctggtatt tcccccgttg gtgccgtcga tatagcgttc ttcagccgtc gcgcggatgc
      961 tcttgccgaa atagcgcatc gcgccgccca aagtcagttt gttgcccaac cagcgcgtac
     1021 cgacttccaa acgtccgtaa tctcgcggca gggcggaaac cctgctcagc ccataacctt
     1081 gtttgagttg gtcttctttg gaggcattgt tgggcgattc gctcgcatcg ctgaaattgg
     1141 tcggttgcgt gcttttttga taggcgtaag aaaggttggt gaaaaaacgc ccataatcgt
     1201 aattcagctc cagctcaaaa ccgtgtttgt gcactttgtc tttgaaattg cggtgtcgga
     1261 tggtgtaggc aagcccggtg ctgccgaccc agctcggaat atccccgttc aaatcccacc
     1321 atttcccgta aacgttgtgg atgtagttgt caatgcggct gcggtagccg accagtttca
     1381 atcctaatat atcatcttgt tttaacaatc cttttttata ggtattgaag ccaaattgcc
     1441 aagtgtttgc gcgctctggt tttaaggcgg tgtgaacgcc ggagtcgccg atttgggaaa
     1501 aatacatttc ttggatgttg ggcatacggt gtgtgcgcga atagccggcg aacggcatga
     1561 aataatcgcc gaagtccgca ctaatgctga ccgaatggtt gttggcgcgc tttttgccgt
     1621 attttttcaa tacgggttca taaagcccgc agctcgggtc gcaatgttcc ttgtatgccg
     1681 gcgagttttc tccgaatgcc cgcttaaatt cgctttccga gccgtaatag cccgtatatt
     1741 cgccgccgaa acggtagttg attgcattgg tgctgtagtt taagcggtaa atgtcttttt
     1801 tgagcgcggc atcgaagtag aacgtgttga aatattgact gccggccggt tggacaatgg
     1861 ttgatttttg gggcagcagc cctttatcgc ccttaaaccg ccccaaatag gaataaagcc
     1921 cgttgtcctg atccggaccg tcgaaaaaca gccccaattc ttcaggaaag cggtttttgc
     1981 cgtattcgtt gtggaaataa ttgaagccca aagtggtttg caactcggtt tcgcggggca
     2041 gccggaaggt ggcggtgttg ttgaggtcga ggatttttgc gttgttgtag gtttcaaaat
     2101 cctttaaaag cccccagcct gtaaacttcg accctttcgg atatttctgc ctgcccgaat
     2161 tgtaggctgc ggtcagattg aggttggtat acgggttcaa agacaaaccg taattgaatt
     2221 gataattgcg gttgatgatt ttgcggctgc cgattctggt gtttaaatcg cgaaattgcg
     2281 ccgtgtattt attgaatacg ccgtcgtatt ccaatttaaa cagattgcct gccgactgct
     2341 gcttcaggcc ggacggatcg atgggggtga tgtcgtattg cggcgccagg ttttcccgcc
     2401 agcttttatc atgctcttcg atgtattttt gcagttcttg ggggtcttcg tattttttat
     2461 accactttgt tttccagtat tgcctttgca aatcccgctc ccattttccg ctgttggaat
     2521 tgaatttcaa cccgccttct tgtacaaaat attgctgttt gcgccgttcc agatattctt
     2581 caccaaaatt tccgatgtgc tgcccgccgc cgcccacgcg gtaattttgc gccacgccgc
     2641 gcctgctgtg cccgtaaagc acaccgacag atgctccgct ttccagccat ttgcgcgcac
     2701 ctatcgccgc catcgcattg cctttggttg aattggtgcc ggtcagacct tttagcagca
     2761 ggccgtaggt attattgccc tgaacgacgt catccacgcc taaagtccgc agattcgccg
     2821 aaccggcaag gctgttgatg cctgccgagc cgctgaagct gcctttgacg acatccagtc
     2881 cggcaataaa attgctgtcg acagatgcac cgaattgaga tgaaccgcct gccctgcccg
     2941 catcggtaga agtcgaataa aaggtctgcg tgatgccgtc caccatcgta ttgacccgcc
     3001 cgaacccgct gtcgccgcga atattcaaag acacaatgcc cgagctttta tcttgctgtg
     3061 taaacgcacc gggtatgctg cgtacgatgt tgtcgaggtt ttcgccggat ttgaacacat
     3121 cctgacgggt cgatacggca cgcgcatcgg taaacacttt tttgtctttc ggtacgcgct
     3181 tcgccttgac gtgcacgtct tccaaaacct gtatctgcgc ctcgctgccc gcgcgccctg
     3241 catcttctgc ataactatga tgatatagca taacacccat aagataaaaa caaatcggct
     3301 tcaaccggaa agaagatctc atattttcct caacaataaa caatcaatcc aacatgtcta
     3361 ccgttttcat cgaatccgcc ctttcgacca cccggcccta cgcaaccgaa ccgtcattcc
     3421 cacggaagtg ggaatctaga atctcggggt ttcagtcatt tccgatagat tcccgccgcg
     3481 tcaggggtcc
//
LOCUS       DAXDZG010000110         3480 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXDZG010000110 DAXDZG010000000
VERSION     DAXDZG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.110"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            98..3297
                     /gene="nalP"
                     /locus_tag="ACFB78_001972"
                     /pseudo
     CDS             98..3297
                     /gene="nalP"
                     /locus_tag="ACFB78_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
BASE COUNT          937 a          948 c          947 g          648 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttt aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagcccccga atctgcatac cggagacttt tcaaacccaa
      421 atgaccaata ttaagaatat gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      481 cgcggggtag aggtaggtat cgtcgataca ggcgaatccg tcggcagcat atcctttccc
      541 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacaa attacaaaaa
      601 ctatacggcg tatatgcgga aggaagcgcc tgaagacaga ggcggtaaag acattgaagc
      661 ttctttggac aatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccatgt
      721 aaaagaaatc ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg
      781 cagacctgca ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg
      841 aaccaagaac gagaaataat gtctgcagcc atccgcaatg catgggtcaa gctgggcgaa
      901 cgtggcgtgc gcatcgtcaa taacagtttt ggaacaacat cgagggcagg cactgccgac
      961 catttccaaa tagccaattc ggaggagcag taccgccaaa cgttgctcga ctattccggc
     1021 ggtgataaaa cagatgaggg tatccgcctg atgcaacaga gcgattaggg caacctgtcc
     1081 taccacatcc gtaataaaaa catccttttc atcttttcga caggcaatga cacacaagct
     1141 cagcccaaca cactgaccct attgccattt tatgaaaaga cgctcaaaaa ggcattatca
     1201 cagtcgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1261 ctgccatgtg gtgcctgtcg gcatcctatg aagcaaccgt ccatttcacc cgtacaagac
     1321 cgattcaagt tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1381 tgcagagata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acgacggctc
     1441 aggacatcgg tgcagtcgga gtggacagca agttcggctg ggggctgctg gatgcgggta
     1501 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1561 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggt ctgatcaaaa
     1621 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1681 aaggcggttc gctggtgtta tcggatatgc acgtcaaaac caaaggtgca ctgatttata
     1741 acggggcggc atccggcggc agcctgaaca gccacggcat tgtctatctg gcagataccg
     1801 accaatccgg cgcaaacgaa accgtacaca tcaaaaggcg gtctgcagct gggcgacaaa
     1861 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gattaccgac
     1921 ggcaagctgt acatgtcggc acgcggcaag ggggcaggct atctcaacag taccggacga
     1981 catgttccct tcctgagtgc cgccaaaatc gggcaggatt attctttctt caaaaatatc
     2041 gaaaccgacg gcggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2101 ggcgacacgc cgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2161 gcacattccg cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa
     2221 aacctgatgg tcgagctgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact
     2281 gcggtcgccg accgcacaga tatgccgggc atccgcctac ggcgcacaac tttccgcgca
     2341 gcggcagccg tacagcatgc gaataccgcc gacggcgtac gcatcttcaa cagtctcgcc
     2401 gctaccgtct atgccgacag tgccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2461 gccgtatcgg acgggttgga ccacaacggt acgggtctgc gcgtcatcgc gcaaacccaa
     2521 caggacggtg gaacgtggga acagggcggt gtcgaaggca aaatgcgcgg cagtacccaa
     2581 actatcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcata
     2641 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     2701 gcaggcatac ggcacgatgt gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     2761 cgctacaaaa acagcatcag ccgcagcacc ggtgcggatg aatatgcgga aggcagcgtc
     2821 aacggcacgc tgatgcagct gggcgcactg ggtggtgtca acgttccgtt tgccgcaacg
     2881 ggagatttga cggttgaagg cggtctgcgc cacgacctgc tcaaacagga tgcattcgcc
     2941 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga
     3001 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtc tgcgacggcg
     3061 ggcgtggaac gcgacctgaa cggacgcgac tacgcggtaa cgggcggctt taccggcgcg
     3121 gctgcagcaa ccggcaagac gggtgcacgc aatatgccgc acacccgccg ggttgccggt
     3181 ctgggggtgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacacc
     3241 ggttccaaac agtacggcaa ccacagcgga caaatcggcg taggctaccg gttctgacgg
     3301 acagaaaaca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3361 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat
     3421 cagacgtata gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacggat
//
LOCUS       DAXDZG010000111         3165 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXDZG010000111 DAXDZG010000000
VERSION     DAXDZG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.111"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..2330
                     /locus_tag="ACFB78_001973"
     rRNA            <1..2330
                     /locus_tag="ACFB78_001973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2427..2540
                     /gene="rrf"
                     /locus_tag="ACFB78_001974"
     rRNA            2427..2540
                     /gene="rrf"
                     /locus_tag="ACFB78_001974"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2576..2695
                     /locus_tag="ACFB78_001975"
                     /pseudo
     CDS             2576..2695
                     /locus_tag="ACFB78_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
     gene            2797..>3165
                     /locus_tag="ACFB78_001976"
     CDS             2797..>3165
                     /locus_tag="ACFB78_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC1269895.1"
                     /translation="MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLF
                     SCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPA
                     VLAKLKDQASVGAPAPKGPAK"
BASE COUNT          804 a          710 c          957 g          694 t
ORIGIN      
        1 accttttgta taatgggtca acgacttaca ttcagtagcg agcttaaccg gataggggag
       61 gcgtagggaa accgagtctt aatagggcga tgagttgctg ggtgtagacc cgaaaccgag
      121 tgatctatcc atggtcaggt tgaaggtgcc gtaacaggta ctggaggacc gaacccacgc
      181 atgttgcaaa atgcggggat gagctgtggg taggggtgaa aggctaaaca aactcggaga
      241 tagctggttc tccccgaaaa ctatttaggt agtgcctcga gcaagacact gatgggggta
      301 aagcactgtt atggctaggg ggttattgca acttaccaac ccatggcaaa ctcagaatac
      361 catcaagtgg ttcctcggga gacagacagc gggtgctaac gtccgttgtc aagagggaaa
      421 caacccagac cgccggctaa ggtcccaaat gatagattaa gtggtaaacg aagtgggaag
      481 gcacagacag ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct
      541 cactggtcga gtcgtcctgc gcggaagatg taacggggct caaatctata accgaagctg
      601 cggatgccgg tttaccggca tggtagggga gcgttctgta ggctgatgaa ggtgcattgt
      661 aaagtgtgct ggaggtatca gaagtgcgaa tgttgacatg agtagcgata aagcgggtga
      721 aaagcccgct cgccgaaagc ccaaggtttc ctacgcaacg ttcatcggcg tagggtaagt
      781 cggcccctaa ggcgaggcag aaatgcgtag tcgatgggaa acaggttaat attcctgtac
      841 ttgattcaaa tgcgatgtgg ggacggagaa ggttaggttg gcaagctgtt ggaatagctt
      901 gtttaagccg gtaggtggaa gacttaggca aatccgggtt ttcttaacac cgagaagtga
      961 tgacgagtgt ctacggacac gaagcaaccg ataccacgct tccaggaaaa gccactaagc
     1021 ttcagtttga atcgaaccgt accgcaaacc gacacaggtg ggcaggatga gaattctaag
     1081 gcgcttgaga gaactcggga gaaggaactc ggcaaattga taccgtaact tcgggagaag
     1141 gtatgccctc taaggttaag gacttgctcc gtaagccccg gagggtcgca gagaataggt
     1201 ggctgcgact gtttattaaa aacacagcac tctgccaaca cgaaagtgga cgtatagggt
     1261 gtgacgcctg cccggtgccg gaaggttaat tgaagatgtg caagcatcgg atcgaagccc
     1321 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     1381 ttccgacccg cacgaatggc gtaacgatgg ccacactgtc tcctcccgag actcagcgaa
     1441 gttgaagtgg ttgtgaagat gcaatctacc cgctgctaga cggaaagacc ccgtgaacct
     1501 ttactgtagc tttgcattgg actttgaagt cacttgtgta ggataggtgg gaggcttgga
     1561 agcagagacg ccagtctctg tggagtcgtc cttgaaatac caccctggtg tctttgaggt
     1621 tctaacccag acccgtcatc cgggtcgggg accgtgcatg gtaggcagtt tgactggggc
     1681 ggtctcctcc caaagcgtaa cggaggagtt cgaaggttac ctaggtccgg tcggaaatcg
     1741 gactgatagt gcaatggcaa aaggtagctt aactgcgaga ccgacaagtc gggcaggtgc
     1801 gaaagcagga catagtgatc cggtggttct gtatggaagg gccatcgctc aacggataaa
     1861 aggtactccg gggataacag gctgattccg cccaagagtt catatcgacg gcggagtttg
     1921 gcacctcgat gtcggctcat cacatcctgg ggctgtagtc ggtcccaagg gtatggctgt
     1981 tcgccattta aagtggtacg tgagctgggt ttaaaacgtc gtgagacagt ttggtcccta
     2041 tctgcagtgg gcgttggaag tttgacgggg gctgctccta gtacgagagg accggagtgg
     2101 acgaacctct ggtgtaccgg ttgtaacgcc agttgcatag ccgggtagct aagttcggaa
     2161 gagataagcg ctgaaagcat ctaagcgcga aactcgcctg aagatgagac ttcccttgcg
     2221 gtttaaccgc actaaagggt cgttcgagac caggacgttg ataggtgggg tgtggaagcg
     2281 cggtaacgcg tgaagctaac ccatactaat tgcccgtgag gcttgactct atcatttgaa
     2341 ggacttcaag agataaaagc ttactgactg attcagtcat taccgaatat attgattaag
     2401 gctttaccga tttgcaacag tttaagtttg gcggccatag cgagttggtc ccacgccttc
     2461 ccatcccgaa caggaccgtg aaacgactca gcgccgatga tagtgtggtt cttccatgcg
     2521 aaagtaggtc actgccaaac accctttcag aaaacccccg gatatccggg ggtttttgct
     2581 ttgcccggaa aaaatgtcgg ggatggcggg acggcatctg tacggtgtcc ggtcgggttt
     2641 gcggaggaac ggcttgaaac tttgggatat tcattttaga atgacccgtt ttatagcgga
     2701 ttaacaaaaa tcaggacaag gcggcgggcc gcaggcagta cggatagtac ggaaccggtt
     2761 cgcccggtgc ttcatcacct tagggaaccg ttccctttga gccggggcgg ggcaacgccg
     2821 taccggtttt tgttaatccg ccatatcgtc gcaagatgcg gtttgttgtt tgcaaccctt
     2881 aaaggaaaaa ccatgaaaaa aatgtttgta ttgttctgta tgctgttctc ctgcgccttc
     2941 tcccttgcgg cggtaaacat caatgcggct tcgcagcagg agctggaggc gctgccgggc
     3001 ataggcccgg cgaaggcgaa ggccattgcg gaataccgcg cgcaaaacgg cgcgttcaag
     3061 tctgtggacg atttgatcaa ggtgaagggc atcggtccgg cggtgctggc gaagctgaaa
     3121 gaccaggctt ccgtcggcgc gcccgcacca aaaggccccg ccaaa
//
LOCUS       DAXDZG010000112         3111 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXDZG010000112 DAXDZG010000000
VERSION     DAXDZG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.112"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(205..642)
                     /gene="rnhA"
                     /locus_tag="ACFB78_001977"
     CDS             complement(205..642)
                     /gene="rnhA"
                     /locus_tag="ACFB78_001977"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC1269896.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            755..1624
                     /gene="tehB"
                     /locus_tag="ACFB78_001978"
     CDS             755..1624
                     /gene="tehB"
                     /locus_tag="ACFB78_001978"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC1269897.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFY
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            complement(1682..2488)
                     /gene="thiD"
                     /locus_tag="ACFB78_001979"
     CDS             complement(1682..2488)
                     /gene="thiD"
                     /locus_tag="ACFB78_001979"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC1269898.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2548..2700
                     /locus_tag="ACFB78_001980"
     CDS             2548..2700
                     /locus_tag="ACFB78_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269899.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            complement(2916..2991)
                     /locus_tag="ACFB78_001981"
     tRNA            complement(2916..2991)
                     /locus_tag="ACFB78_001981"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2955..2957),aa:Glu,seq:ttc)
BASE COUNT          688 a          770 c          915 g          738 t
ORIGIN      
        1 gtcattcccg caaaaaacag caactcgaaa cactccgtca ttcccgagca ggcgggaatc
       61 tagaactttt aaattttcag acggcttttg aatattgccg ttgcccgacg ttctggattc
      121 ccgcctgcgc gggaatgacg gtggaggacg atgccgtctg aagcccatta gcggtttcag
      181 acggcatttt tgtcggtgcg gcaatcaaga aaactgcgcc gcgccacggt ttgccaaatc
      241 gtcggcgcgt tcgttttccg cgtgtcccgc gtgtccttta acccaagtcc agctgacttg
      301 atgctgcccg accagagcgt cgagttcttg ccacaagtcg tcgtttttga cgggctgttt
      361 ggcggcggtt ttccagccgt tgcgcttcca accgtgtatc cagttttcca tgccgttttt
      421 gacgtattgc gagtcggtgc agatgatgac ggtgcagcgg cgtttgagcg atttcagccc
      481 ttcgataacg gcggtcagct ccatgcggtt gttggtggtt tgcgcttcgc cgccgaaaag
      541 ttctttttcg cggctgccgt agcgcattaa tacgccccag ccgcccgcgc cgggattgcc
      601 tttgcacgcg ccgtccgtgt agaggtaaac gggtgtgtcc atatcgtgcc tttgtgtggt
      661 gtccggcgtg attataagcg gtttgtgcgt cgtgccgtct gaaagattgc ggcggattga
      721 aataggcata taatgtgcaa ctttattttt gaatatgaag gagcggatag tgggtcaaag
      781 tggcgagttg ttttgtttcg gacaaatgcc tgtatggaaa gtggaaaacc tgccggaagt
      841 tttattgtcg ggttattcgt ccgaggaagg ggaatgggtc tgcctgaatg tgttgcaggg
      901 cgatgtcgaa gtccgtgcac cggacggggc ggcagaggtt tggtcggcgg aaagcggcga
      961 ttgcgtgttt gcgccgcagc aggtgttttc ggtcaaaccg aaaacggacg atgccgaaat
     1021 ccgtttgtcg ctgtattgcg cggcggcaga ctatttttac aaaaaatacg ggatgagtgc
     1081 cacgcattct gcggtcgcgg cggcacagga taccgtaccg gcgggcaggg cgttggatat
     1141 gggctgcgga caggggcgca acgcgctgtt cctgggtttg aaggggtttg atgtaaccgc
     1201 tgccgattgc aatcccgccg cgttggcaaa cgtggcggaa ctggcagagg cggagggttt
     1261 gaacgtccgc acgctggaat atgatttgaa cgccgccgcc cttcagggcg agtttgatta
     1321 tattgtggca acggtggtgc tgatgttcct gatgccgcag cgcgtgcccg acgtgattgc
     1381 cgatatgcag gcgcatacgg cggcgggcgg gtacaactta atcgtatcgg caatggatac
     1441 ggcggatttc ccctgcccga tgccgttccc cttcaaattt aaagagggcg agctgaagga
     1501 ttattatcgg gattgggagc tggtcgaata taaagaagaa ttgggcgcga tgcacgccaa
     1561 agacgaaaac ggcaacccga tacggtttaa atttgtaact atgctggcga aaaaaccagg
     1621 ataagccgcg ccggacgatg ccgtctgaac ccgaaaaggc gttcagacgg catttttacg
     1681 tctagtcccg atacgcccaa tgattgaccg gcccgtgtcc cgcgccgatt tccaaagggt
     1741 ttgagattgc cgccgtgatg taggccttgg cagtctgtac ggctttgcaa acgtctaagc
     1801 cttttgccaa ctcggcggta atgcaggcgg aaaacgtgca gcccgtgccg tgcgtgtggg
     1861 cggtcggaaa gcgcgggctg tccaattcca gcgtttcgtt ttgcgtaaac agccaatccg
     1921 tgcagcgtcc gcttgtgctg ccgttcaaat gtccgccttt gataatgaca tttttgacac
     1981 cgtaatcaag caggattttt gccgcacgtt ccgcatcttt acggttttcg atatgcacgc
     2041 ccgtcaaggc ttccgcctcg ggcaggttgg gggtcaggat gtccgtgtcc ggcagcagca
     2101 ggcgcgtcag tgccgcaacg gcggaatcct gcaacagcgg cgcaccgcct ttggcaatca
     2161 tcacagggtc gagtacgcgc ctgccaaagc tgcagtgttt cagcttgtcg gcaacgcatt
     2221 cgatgatttc cgccgtgccg agcataccga ttttgtaggc gcggatgtca aagtcttccc
     2281 tgattgcttg gatttgtgcg gtgatggttt cggtcgggac gagatgaacc gccgacacgc
     2341 ccaaggtatt ttgcgcggta acggcggtga tgacacacgt tccgaacacg ccgcgcatct
     2401 gaaacgtttt caaatccgcc tgaatgcccg caccgccgcc cgaatccgaa ccggcgatgg
     2461 caagcgtctg cacaaaagag cctttcattt gttcctcctt aatattgcgt ccgataaaaa
     2521 gggctactgc ctgtaaaaaa gcaggcaatg ccgtctgaaa accgcagcgc ggggtttgtg
     2581 gtgcattatt atgtcgtatt gcaggacaag gcacagggca aggcagcggg gtgcatcgta
     2641 gcaactgata atgaaaaaag agggcgatgc cgtctgaacc gcgtgttgcg gcgagtataa
     2701 accggcacgc ttcattcgtc attcccgcaa aaacagaaac ctaaaattcc gtcattcccg
     2761 cgcaggcggg aatccggacc ttagaacaac agcaatattc aaagattatc tgaaagtttg
     2821 aggttctaga ttcccgtttt cacggcaatg acgaaaaatt gcgggaataa cgattcataa
     2881 taaaaaacct gcttgaaaca agcaggtttt tgaattggca cgcccacggg gattcgaacc
     2941 ccggttgccg ccgtgaaagg gcagtgtcct aggcctctag acgatgggcg cgtggaaacg
     3001 gacattatac atacttatag aaatttagca atacgatgcc gtctaaacga atgtcagggg
     3061 gcgtttgtct gaccgtatca caagataaaa aacattggaa tatcaagcaa t
//
LOCUS       DAXDZG010000113         3077 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXDZG010000113 DAXDZG010000000
VERSION     DAXDZG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.113"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(199..558)
                     /locus_tag="ACFB78_001982"
     CDS             complement(199..558)
                     /locus_tag="ACFB78_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269900.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(561..1160)
                     /gene="mafB"
                     /locus_tag="ACFB78_001983"
                     /pseudo
     CDS             complement(561..1160)
                     /gene="mafB"
                     /locus_tag="ACFB78_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            complement(1216..1497)
                     /locus_tag="ACFB78_001984"
     CDS             complement(1216..1497)
                     /locus_tag="ACFB78_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269901.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            complement(1475..1999)
                     /gene="mafB"
                     /locus_tag="ACFB78_001985"
                     /pseudo
     CDS             complement(1475..1999)
                     /gene="mafB"
                     /locus_tag="ACFB78_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            complement(2047..2382)
                     /locus_tag="ACFB78_001986"
     CDS             complement(2047..2382)
                     /locus_tag="ACFB78_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269902.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            complement(2379..>3077)
                     /locus_tag="ACFB78_001987"
     CDS             complement(2379..>3077)
                     /locus_tag="ACFB78_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1269903.1"
                     /translation="AHIQAGDRVLSKDEASGETGYKPVTARYGNPYRETVYIKVSDGI
                     GNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVVKPKPLKAYNLT
                     VADWHTYFVKGSQAETEGVWVHNDCPYGKGNQRYKDAPYHGKNDNSVKSRAPTNGQAV
                     LDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNWKNLHTDQQNAL
                     KKAGLVNSKGKIKK"
BASE COUNT          803 a          650 c          552 g         1072 t
ORIGIN      
        1 gggcaggtgt aggattttta aagaacttag ttctcaatat cctgtttcat tcatcaaaat
       61 gccgtctgaa agctgaatac cgcttcagac ggcattttgg tggttgggtt tttaagccaa
      121 cctacgctta ctgaaaacca aattgagttt cagacagttt ttaggtttgg gtgtccaatc
      181 taacttatat ttgtccattt aattagtcgt ttgtatcaaa tttccattat ttattttcca
      241 atttacttta taattatctt cataataatc taattcaaaa aaacctgata tttcaatatc
      301 caattccatt attgttttaa tacatttttc aaaataaata atgaaataag attttacgca
      361 tgcaccaaaa aaaatatagc tgctccaatt aaaactattt gtcgggaaaa cccacccgct
      421 tttatatatt tttgcagatt ctttctcttc gatattaaag ggacaattat tccaaaaatt
      481 attaatgttt tcttggatga tttttatatc atcgtcagag cattcaatcc atccttttat
      541 ggaaacatat gatgccatgt ttaatctcct aaacctgttt taacaatgcc gccttttgat
      601 tcaatatatg acttaacttg tgaatgaaca ccgtatttaa accaaaattc tgcacgtttt
      661 ccctgttggt ttgctgcttc gatggttgct ttaatttgct ttctattttt ttgatttaag
      721 aaatttttag gtttatctat tgctgaaatt gttcttttgg cttgtattaa agcatcattc
      781 gtaacagcgt caatttctct accgttaaca agttttgatg taccatcggt ttttcttcta
      841 attaaatctt cataatgtat atctagagct tctctatact ttgcattttg atataactgt
      901 ctcgcactat cagacaaagc caatttcttt ttataagaat cagcaaaatc cccgctaacc
      961 gcagccttcc ctggttttgc cgcctttgcc aacttcgcga ctttggctgc tgcggcaacg
     1021 ttgaagacgg cttcgacggt ttcggcggca ttgggatttt cctgtatcca ccggtcaacg
     1081 gcttcgcgta tggaattttc tctatcagag agataactca atttcccgat agctgtaaat
     1141 tcataatccg ccgatatggg gaaaggtcgt ctgaaaatta aaagcagttt cagacgactt
     1201 tttgatttat gcttgttacg aaaagtcttc taaatgatgg ctaattgcct tttcaatagt
     1261 tggataccaa tccataaatg aagactgtaa ttcctctaaa accaactcat tgaacaaatc
     1321 atctaaattt ctccaatcca ttttttcatt tggatagagg aaattcgcca taacgacagt
     1381 atgtttattt tgggagtaat ccgatagggc gcaaatcatc agatactgcc ccgaatgctc
     1441 cagataaaaa attctttttt ctaaggaaga ttgattattt tggtgtccat ttcccatttt
     1501 ttaccctttt tgacgtattg gctttcgagt tcttaaattg agttacctgt cttccatcag
     1561 gagttagaat aaccacttta tctcctttta cataaacatg gtttcctgtg tcggaatcta
     1621 aatattgctt tccttcgcga acgacacgat tggaatctcc cacacttcga tgagaatcgt
     1681 ttttagcttc ttcttttctt tgttgggcat gattggttgg ttttggttta ggcggacaat
     1741 cattatgaac ccaaactcct tccgtttccg cctgactacc cttgacgaaa taagtatgcc
     1801 agtcggcgac ggtcagattg taggctttga gcggttccgg tttgacggta acgctttgaa
     1861 cggtctgctc tgcaccgttt tcagcaaaca gcctgcttcc cgctttcaaa tcttccgctt
     1921 taatccattt gccgtccgaa taaaacggat ggatgcggtt ggaaatcagg gtttggctgt
     1981 taccgatgcc gtctgaaagc cggaatgtac tttcagacgc atcggatttc cgggtttaat
     2041 ccgcgcttac ggacgctttt taaatataaa gaaggaaata tcgtctaacg catcttcggc
     2101 aaaaccttga aaatccggat taccttgttt tgagcgcaat aaagcaatca ctttatcgca
     2161 atcgccgata ttttcatgca tcctggcgag atgtccgaaa caggttaatg acaatctggc
     2221 aatatccaag tctttatgct tcgacaactc cgtcaatttc tcggaaatcc aagggatttc
     2281 atcctcctcc gagctgaagg tcataccgag cataccatca ataacacaat ctttattttc
     2341 agaataaatc atcttttcta cttgttcctt atcagccatc attttttgat ttttcctttt
     2401 gaattaacca atcctgcttt ctttaaagca ttttgttgat cagtatgtaa atttttccag
     2461 ttccttacat gcccatgata ctcagcagaa ccgtcattaa aaattctagt ttggtttaat
     2521 acaacgattt cattattggt tttatcaact ccaactcttt gagaagatgt cgatttaact
     2581 tgaacagagt tatcaaggac agcttgccca tttgttggcg ctctactttt cacagaatta
     2641 tcatttttac catgataagg agcatcttta tatcgttggt tgccttttcc atacggacaa
     2701 tcattatgaa cccaaacccc ttccgtttcc gcctgactgc ccttgacgaa gtaggtatgc
     2761 cagtcggcaa cggtcagatt gtaggctttg agcggttttg gtttgacaac ggttttgcgg
     2821 acggtttggg ttctgccgct ttcggataac agtctgcttc ccgcttttaa atcttccgcc
     2881 ttaatccatt tgccgcccga ataaaacgag tggatgcggt tggaaatcag ggtttggctg
     2941 ttaccgatgc cgtctgaaac tttaatgtaa acggtttccc gatacggatt accgtatcgg
     3001 gcggtaacgg gtttgtatcc cgtttctccg cttgcctcgt ccttggaaag gacgcggtct
     3061 ccggcttgaa tatgggc
//
LOCUS       DAXDZG010000114         3066 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXDZG010000114 DAXDZG010000000
VERSION     DAXDZG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.114"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(109..300)
                     /locus_tag="ACFB78_001988"
     CDS             complement(109..300)
                     /locus_tag="ACFB78_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269904.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(415..870)
                     /locus_tag="ACFB78_001989"
     CDS             complement(415..870)
                     /locus_tag="ACFB78_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269905.1"
                     /translation="MEKIINSKVRETNFNQAWRVIRAMQINNISDEQVMIVKMSCYLK
                     KDEIFKNIERYGYQSSDIKKINPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LIFEIFILDRESIKYIDRDKLFKPGETLYLNFKSIKNQCKNYFFLRFWF"
     gene            complement(858..1196)
                     /locus_tag="ACFB78_001990"
     CDS             complement(858..1196)
                     /locus_tag="ACFB78_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269906.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            complement(1241..1618)
                     /locus_tag="ACFB78_001991"
     CDS             complement(1241..1618)
                     /locus_tag="ACFB78_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269907.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            complement(1676..2041)
                     /locus_tag="ACFB78_001992"
     CDS             complement(1676..2041)
                     /locus_tag="ACFB78_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269908.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(2023..2562)
                     /locus_tag="ACFB78_001993"
     CDS             complement(2023..2562)
                     /locus_tag="ACFB78_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269909.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(2605..>3066)
                     /locus_tag="ACFB78_001994"
     CDS             complement(2605..>3066)
                     /locus_tag="ACFB78_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFC1269910.1"
                     /translation="KDGKNPALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV
                     LSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRID
                     GKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTF"
BASE COUNT          919 a          675 c          524 g          948 t
ORIGIN      
        1 taaagaaacg gcaacacacg ccaacagaaa aacatatttg aacttcatca tattttccac
       61 ataaaaggca gcctgaaaat ctttcaggct gcccttgtca aattattcct agctttcggc
      121 ttttttggca aaccaaacaa tccgattacc cgcataatac tttccattta ttgaaatccg
      181 acaagccgcg cccaaaaaat gccatgcact gccgatttcc gcagcaatct ttgtaccgtt
      241 ttcttcaaat tccaaatatt cacccaataa taaacttgaa acagaacacg cgggcagcaa
      301 gtgcccaccc tacgcttatt caaataattt gattaaataa agaataaaga aaaggcatca
      361 ggaacaaaat tataatctgc cacttgactc gcaccgcttt taaagtaagg ggcatcaaaa
      421 ccaaaaccgc aaaaaaaaat aatttttgca ttgattttta atagatttaa aattcaaata
      481 tagtgtttct ccaggtttaa aaagtttatc tctatctata tatttgatgc tttccctatc
      541 caaaataaat atttcaaata ttaaaaaatc attacatgac caagccaaat cataaatttg
      601 attcaaatgg gtatcataaa gataaaaata ataaggtttg ggaacaggta aaatatttaa
      661 tggaaaagga ggattaattt tcttaatatc tgatgactga tatccatatc tttctatatt
      721 cttaaaaatt tcatctttct taagataaca agacattttg acaatcataa cctgttcatc
      781 agatatatta tttatttgca tggcgcgtat aacacgccat gcctgattaa aattagtctc
      841 ccttacctta gaatttatta tcttttccaa agatgaaaat gccccttatc ttgaactctc
      901 cagttagaac ctggaagttt cgtacctctt aaatgtgtat taatatttct tatagtttca
      961 ttaaaatgcc acgcgctgcc gatttcaacg gtaattttcg taccgctttc ttcaaattcc
     1021 aggtattccc ccattagcga acgcaaagaa gcagacgcca tcccggcttc gttatgataa
     1081 acccgcctgc cgttgatata gacttccgcc cctgtccgtt tcaaattcca aaaattccgt
     1141 actgaaattt ccatatcccg atattgtgca gaccatgttt tttcgaaggt tttcataaaa
     1201 tttcctatac ctgtccaatc ggcacatatc aattgcatta ttacatctca atacgataaa
     1261 tatttcttaa gtcaaaatgc aagcctgacc gtacctcaac tgtcaaaatt ttattatttt
     1321 ttattgattt taagacaatt tctgaaaaat tctcttcgct ttctcccttt tgtagaagca
     1381 catcagaaaa aataaaactt tcccgattaa attcataaat atgtttcaac cattcacctc
     1441 ctctttctgt aaggcaagat tcagtttcat tcttccttat tgtataaata tttccttcac
     1501 aaaatctgaa ataaatccat aaatccatct tatccataat taaagaaaaa gtttcacctc
     1561 gagattttgt caacaattcg caaggttgcg atgttgcaat caaatagccg aaagacattt
     1621 tttacctcat acatggtcga aatcagtttc tgttagttca gaatccattt tttcgtcaac
     1681 aactgaatcc gcatttttga attaacgttt tcatcagctg ccgtttatct aaaccggcag
     1741 gttcagtttc agaataagcc ttatatgaag actgtaagca tttcagaaaa agatcatcag
     1801 aaggcatatc tgccgaatca aatacaactg ttttgatttt ggtacttacc caaaaccctt
     1861 tttgctcttt ttctactata cggaaattca gaatatttcc aaccgaatca aaagcacggt
     1921 aaacatcatc catcaaatcc tgcggctcta ttttcttttc caattccgac aatccttgaa
     1981 atatatccaa agacacatct tcaaatagaa aaaaaggagg agttagaagc ggtttttcca
     2041 tgatctgtcc gtagattttg attcccaagg gcgatgacga ccaattccct gtccaggcaa
     2101 agtcttgccc gtattatccg taactcgacg atgataatgg ggaaattttc caataggatg
     2161 acctgttcta ttaccgaaag gggctatccg catattattg ccgattttaa tctcacgtcc
     2221 atatttagca aaggaaacaa cctttcctgc agcacctaca tcaccaggaa ttgcgcctaa
     2281 tccgccagca acatctctaa cagaagctgg tctgcctgtc gttgcataac taaaaccatg
     2341 ctgtgtccac ataccaatgg cagcaccacc caagatagcc aatggaagaa aagccccttc
     2401 agtctccttc atctccttct gagaaagctc cgccaactgc atcggcgcat ctgcccgcgt
     2461 gtggaacact tggtcttcaa atgcctgatt gtccaatccg tttgccattg cgggggcgat
     2521 catagacagc atcattacgg ctgcggtgat ttgttttttc ataataactc ctttggatta
     2581 caaggttgga aaatcaaaat ctgcttaaaa tgtatgctgt acgcccaatt tcagttcgga
     2641 actgctttgc cctgaaacgt tgaaacgtgc ggatgcgttt aaagccgcgg ttttggtgaa
     2701 accgaaacct gcgccgaaat gggcgtaggt ggatgtgttt cttgcggatt cttttttgcc
     2761 gtctatgcgg tcgggctgtt tgcccagcca ttggatgcct ccggtcaggc tgattctgtc
     2821 gttggcggca aatgagatgt tgggattcag catccagtaa ttgcctgctt tgtatttgac
     2881 gtcgtctgaa agggttttgc tgccgttgat gcggtaggcg gcggtgaggg aaaggacaat
     2941 cggatctatg gctttgtagg tggtggcgcc gatgagccac gattttcccg acgaggcttt
     3001 gttgcgcgat ttttcgtaaa ccgtgctttc gaggaaagcg atgagtgcgg gatttttgcc
     3061 gtcttt
//
LOCUS       DAXDZG010000115         3028 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXDZG010000115 DAXDZG010000000
VERSION     DAXDZG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.115"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            43..2300
                     /locus_tag="ACFB78_001995"
                     /pseudo
     CDS             43..2300
                     /locus_tag="ACFB78_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            2313..2955
                     /gene="pgmB"
                     /locus_tag="ACFB78_001996"
                     /pseudo
     CDS             2313..2955
                     /gene="pgmB"
                     /locus_tag="ACFB78_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
BASE COUNT          769 a          909 c          773 g          577 t
ORIGIN      
        1 attttaccat tccctcaaat caatccaaac aggagcttta cgatgtacac aagaattatg
       61 gaaatcagcc cttggacgct gcgttcggca aaactggaaa aagaacacaa acggctgcaa
      121 gagagcctga ccagcttggg caacggctat atgggtatgc gcggcagctt tgaggaaacc
      181 tactccgccg acagccactt gggcacctac atcgccggcg tgtggtttcc cgacaaaacc
      241 cgcgtcggct ggtggaaaaa cggctatccc aaatatttcg gcaaagccat caacgcgctc
      301 aatttcagca aagtcaaaat ctttgtcgac gggcaagagg tggacttggc gaaaaacgac
      361 gttgccggct tctccgtcga actggatatg caacacggcg tgttgcgccg ctcgttcacc
      421 gtattcggtg tgcgtttcga tgtgtgcaaa ttcctgtcgg tcgcgcaaaa agagctggct
      481 ctcacccgct gggaagccgt gtccgttgac ggcaaaaccc accaagtccg catcgattcc
      541 atcatcgatg ccgacgtgaa aaacgaagac tccaactacg aagaaaaatt ctggcaggta
      601 ttggacaaag gcgtttcaga cgaccgctcc tacattgccg cccaaaccgt cgccaaccct
      661 ttcggtgtgg aacaatttat cgtcaacgcc gggcaaacct tcgccggcag cttcaaagcc
      721 ttcggcggca gccaaaccga ttggcaggtc tctaattctt ttgaagccga agtcggcggc
      781 acgcccgaaa cctttgaaaa acgcgtgatt gttaccacca gccgcgatta tcagggcttg
      841 gaagcagtga aagccgccgg ccgcgcctta tctgaaaaaa tcacaggtgt tgcgtttgaa
      901 accttgctgg atgcgcacaa agcaggctgg cagcatcgtt gggaaatcgc cgacgtggtc
      961 atcgaaggca gcgacgaagc acagcagggc atccgcttca acctgttcca actgttctcc
     1021 acctactacg gcgaagacgc gcgtctgaac atcggcccca aaggctttac cggcgaaaaa
     1081 tacggcggcg cgacctatcg ggacaccgaa gcctacgccg tgccgctcta ccttgcactg
     1141 gcggaacccg aagttacccg caacctgctg caataccgcc gcaaccaaat tgccgcaggt
     1201 gcagcataac gcacgcgaac agggcttggc gggcgcactc tgtccgatgg taacgtttac
     1261 gggcatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gtaacggtgc
     1321 gattccttac gccatctaca actacaccaa ctacaccggt gacgagtgct atcttgccaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttccgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcaagg cgtaaccggt ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaaa ccaacaccct cgccgcatgg gtattgggct acacccgcga
     1561 agccttggcg aaatacccgc gtccgggttc gaacgtgagc gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gtatgacgaa gaactcggcg tattcgtgca
     1681 gcacgacggc ttccttgaca aagacatccg ccccgtatcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaaacaggc
     1801 ggacgtattg caaggcatct acttcttcgg cgaccgtttc gacatggacg aaaaacgccg
     1861 caacttcgac ttctacgaac cgatgaccgt gcatgaaagc tcgttgtctc catgtatcca
     1921 ctccatcctc gctgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggacg actacaacaa cgacaccgaa gacggcctgc atattacttc
     2041 tatgaccggc tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cttggggcgg
     2101 caaactcagc ttcgcaccgt tcctgccgag tgcgtggaca ggctatgcct tccacatcaa
     2161 ctaccgcggc cgtctgatta aagtcgccgt cggcaaaaaa cgtcgtcttc accctgctca
     2221 aaggcgatcc gctcgaattg caggtgtacg gcaaagacat cacgctcaac ggcagccaca
     2281 ccgttgcatt ggaaaaataa ggatggcgca aaatgacttt taccgcagtg ctcttcgatc
     2341 tcgacggcgt gattaccgat accgccgaat accactacct cgcatggaaa aagcttgccg
     2401 aagaattggg catcggcatc gaccgcaagt ttaacgagca gctcaaaggc gtgtcgcgcg
     2461 acgattcgct caaacgcatc ctcgcgcacg gcggcaaaac cgtcggcgaa accgagttcg
     2521 ccgaactgac ctgccgcaaa aacgacaact acttggagat gattcaggca gtcaaacccg
     2581 aagacgtgta ccccggcatt ttgccgctgc tggaagcatt gagggcaaac ggcaaaaaaa
     2641 tcgccctcgc gtccgccagt aaaaacggcc cgttcttgct cgaacgcatg gggctgaccc
     2701 acttcttcga caccgtcgcc gaccctgccg ccgtcgcgca ttccaaaccc tcccccgaca
     2761 tcttccttgc cgccgccgag ggcgtgggtg cggacatacg ccgctgccgg cgtcgccgcc
     2821 atcaaagcct ctggcgcttt gcccatcggc gtgggcaaaa ccgaagactt gggcagcgac
     2881 atcgcgctgg tgtccgacac cgccgagctg acctacgcct acttgcaaaa tgtgtgggaa
     2941 cagtcgggca gataaaattc taaggaagta aaaaggccgt ctgaacagtg tttcagacgg
     3001 cctttctgct tttagaacag aatgataa
//
LOCUS       DAXDZG010000116         2970 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXDZG010000116 DAXDZG010000000
VERSION     DAXDZG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.116"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            125..373
                     /locus_tag="ACFB78_001997"
     CDS             125..373
                     /locus_tag="ACFB78_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269911.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIINNTVSSDE
                     KLLIINDFLSGFLEFDPETGEPVGETLKIEQMIDFFLV"
     gene            1250..1521
                     /locus_tag="ACFB78_001998"
                     /pseudo
     CDS             1250..1521
                     /locus_tag="ACFB78_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1757..2043
                     /locus_tag="ACFB78_001999"
                     /pseudo
     CDS             1757..2043
                     /locus_tag="ACFB78_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2468..2692
                     /locus_tag="ACFB78_002000"
     CDS             2468..2692
                     /locus_tag="ACFB78_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269912.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            2748..>2970
                     /locus_tag="ACFB78_002001"
     CDS             2748..>2970
                     /locus_tag="ACFB78_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC1269913.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQ"
BASE COUNT          953 a          481 c          596 g          940 t
ORIGIN      
        1 aaagacggca aaaaccccgc cctaatcagc tttcttgaaa gcacggttta cgaaaaatcg
       61 cgcaacaaag cctcgttaat caaaaaaagg gggctttgcc ccttttataa cttaaggata
      121 aattatgaat attaaaaatg aaatagttgc tttaatttct aaaaaaattg atggggggca
      181 atatagttct ttagaaaatt ttgctttatc cgtactaagg gaaaaaatta taaacaatac
      241 tgtttccagt gatgaaaaat tattgattat taatgatttt ttatcaggtt ttcttgagtt
      301 tgatcctgag acaggggaac ctgtcgggga gacgcttaaa attgagcaaa tgattgattt
      361 ctttttagtc taaatcatac ataccagtga acaagctagg cgtatatccg gcttttaaat
      421 acgattgtca ggtttatact tgggataaaa tcaaaaataa agttgtttaa actctagtag
      481 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggaaccga
      541 ttcactcggt gcttcagcac cttagggaat cgttcccttt gagccggggc ggggcaacgc
      601 cgtaccggtt tttgttaatt cactatattg catggtgcat gcattaaatc agtcttctag
      661 cgccgcaatg ggaaaacata gaagataaac gaaatggatg atctaatact atatttttta
      721 tcaggtatat tcggaaatca agttgctgaa tatattataa aaaacaaccg tgagataaaa
      781 gttcccttta ttgcgcttta cgctatattt tttacgttga tttatactgt ggctttgttg
      841 tttctcagtc tgatctattg ggtcaatggt gcggagattg catggaaggg gataggtatt
      901 ttcagtatgt cggtcagttt ttgtatagtc ttctgtcttt atttgattga caaggcagga
      961 agatgtaagg ataagaaaca atagggatca gatgcggcag caaacgtaga ttgggtctag
     1021 gctcaacctg cgtttgctgc atctttggaa gaaatagtgg attcgaaaaa ataggaagaa
     1081 ttctgattaa tggtatgcga ggtgtagcag tgggggctgt tgctggtggc ataactggtt
     1141 atgcaggttc tacgggtaag aacactgaca ttcgccgtta aataataaat aaaccaactt
     1201 ccaaccaaac ctgctatcgg tttggttgga agtgagagga ggtaaggtag tgtttgctgt
     1261 tattttcttt tcgacattgg gctgtatttt agcatggata agagatattc ctaaaataaa
     1321 atcaaaaaaa attttggcaa gatccttata tattatagga ataataaatg taataatcag
     1381 ctatgtatta attaaaaata tattggtttc tgtttcggat ggaggtggaa taaaatatgt
     1441 tgcaatatat ttatcaaatc ttttttttgg acagtattga tgtatgctct tgtgaaaaga
     1501 ttatctaaaa agccaagctg aaacccagta cacaacacat caataaagat tccttttgca
     1561 cggaacaatg acaatattca aattgctgga tttgcaatct gatttaccta ttgaaattgg
     1621 gtgtgcaaca tacgttctaa gcaggctttg atttcccaaa gaggctgaag ggtaagagac
     1681 gcttatatca tagataaata atggcaaaac aggtaatgaa ttaaatgcat tacctgtttt
     1741 tacagaaatt tttaaaatag atgatttgat tgcagctttt taggctattt atcaggtaaa
     1801 tacagtgaag ggaaaataag caggttagaa atatccctaa cggcaggaag cattttcttt
     1861 gcagtattct ttattcatga actgataatt tttattgttg atggaaataa ggaaaatctt
     1921 tggaatttta ttactgggat aatgaaagat ttatttttat tttcatgttc tgtttgttta
     1981 ttattctatt tcttattatt gattgcagaa accattggaa aaacaatcaa taagattaaa
     2041 taagatattt aagccgctaa aaaaccattt gagtatttta tcttctatgc ggcatccatc
     2101 ttggaattga aatactgcaa tcgggaggct aaggcttccg ttgtaacgtt taccatatat
     2161 ttcattattt taatttcatc aaaaaacaaa attcaagatt atacataccg ccaatcgttt
     2221 cttgattgtt ttgtatagtg gagtaaattt aaatcaggac aaggcgacga agccgcagac
     2281 agtacaaata gtacggaacc gattcactcg gtgcttcagc accttagaga atcgttctct
     2341 ttgagctaag gcgaggtaat gccgtaccgg tttaaattta atccactata tatttatccg
     2401 atatatcaac atctctctat cttattccga attttattgt tgaaacttgc attaaaaacc
     2461 atacaagatg atgaagaaca aatatatttt aataaaagca gcaatcggca taaccgccat
     2521 atccatattt tatcttctga ctcaaggaag catcggcaaa actgaagaac catcatattt
     2581 cttgatgttc atgtttctca actcgctttg gtttgaagaa aataaaacag ttatggctgc
     2641 cgttacggca atgattgccg cccactttat atttgtcgca ctatccgatt aataagcaaa
     2701 caaaaaggct gcttggaaat ttcaggcagc ctttcaggag gattttaatg gaacaaaaaa
     2761 ggcggtttgc cgcctccctg ctcctcgccg ccgccctgcc gctttgtgca cattcttttc
     2821 cctttgcgga agaaaacccc atagcctacg gcaaagtcaa aatacagagt tggaaagcgc
     2881 ggcgggattt caatattgta aagcaggatt tggatttttc ctgcggggcg gcttcggtgg
     2941 cgacactttt gaacaatttt tacgggcaaa
//
LOCUS       DAXDZG010000117         2948 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXDZG010000117 DAXDZG010000000
VERSION     DAXDZG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.117"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..414
                     /locus_tag="ACFB78_002002"
     CDS             <1..414
                     /locus_tag="ACFB78_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1269914.1"
                     /translation="WHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGGSQTD
                     GARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNADRAK
                     HARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            404..952
                     /locus_tag="ACFB78_002003"
     CDS             404..952
                     /locus_tag="ACFB78_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC1269915.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            1249..1602
                     /locus_tag="ACFB78_002004"
     CDS             1249..1602
                     /locus_tag="ACFB78_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269916.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1592..1915
                     /locus_tag="ACFB78_002005"
     CDS             1592..1915
                     /locus_tag="ACFB78_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC1269917.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIDGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1974..2201
                     /locus_tag="ACFB78_002006"
     CDS             1974..2201
                     /locus_tag="ACFB78_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269918.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2328..2871
                     /locus_tag="ACFB78_002007"
                     /pseudo
     CDS             2328..2871
                     /locus_tag="ACFB78_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
BASE COUNT          966 a          500 c          612 g          870 t
ORIGIN      
        1 tggcatacct actttgtcaa aggcgataag gcggaaacgg aaggggtttg ggttcataat
       61 gattgtccaa ccaaactaaa accgacagaa cgttataatc gtcaaacgca ttatggaggt
      121 tctcaaacag atggagcaag ggctcaggca gcaagacagg caggagaagg taaaccttgt
      181 ccaacttgtg gtagaattca gatatttggt acgaaaaccg caccaagtcc gcaacatgaa
      241 cccccattag taaaacatta ctatgaacat ggtggtcact ctatgagcaa tgctgatcga
      301 gcgaaacatg ccagggaaag cataaaagga actcaatgtt taacatgtca aaggaaagag
      361 ggtgcgatga tgtcaagata ttcgagagaa caggctaaaa agcatggact ataaccaaac
      421 tgttttatct cacttgcaaa aattttggaa acatcatgat attaaaggat ttacttggac
      481 tttaggaaga attgttgaag aacttcccga tttccaagtt ttccaagtga ttcctaatca
      541 tgaagatgag ccttgggttt atgtcagctc tgggataggt caatttttag ggcaggagtt
      601 ttttatcatc agcccgtttg aaacacctga acatattgag accttggcaa tgctggcatc
      661 tgcaagtatg cattatcctg atcaatttca attagggaaa accgtcaata tcggtagacc
      721 gtgggtcgag caatcatcct tccggcattt tctgatttct ctgccttatc cttatgggca
      781 agagttggaa tatatggata atgtccgatt tttctggtta ttgccaatta cccagacaga
      841 aagacttttc ttgaatactc attcggtaga agaattggaa acgaaatttg atgaagcggg
      901 tattgattat ttagatataa atcgtgcgag tactgtctgg caggcaggat aaaaattcgg
      961 caatcaaaat gccgtctgaa acaagtttgg actttcagac ggtattctga tgaatggaac
     1021 aggatactgg gaactgagtt cttcaaaaat cctatctccg tcccttatac gtcggtgctt
     1081 tagtcaaaac ggcagacgga tgaaaaatta ttgcccgtat ccttgccaaa gatgaagcaa
     1141 gatgaagcca ggtaattgaa tcggtacaag cgcaatgtcc gacttgttct gcaaaacagg
     1201 gagggagatt aagccaatat tcaaaagaac agaaaaagaa actgagtaat gggaataaaa
     1261 aatgaacaaa taataatttg taaaaaatat aatacagaaa tatatcctgt ttccgatgtt
     1321 tcaaaaatcg gtgttgctga aaatgtcaaa cagacaggcc tgtatccgat taacgggtta
     1381 aggcaccgtc ccaaaggtga tactaacggt tggtatatct gggccggtga aaacttctcc
     1441 tatgataaaa attttttcct gcccttgcat acgttccatt tgcaaatatg gcgacccgaa
     1501 atcatcccct ttttgacact tccgcccggt taccgttttt taattgggga aaacggttat
     1561 gaagacgtat ggtttgatga gttgctatta aatgataatt gacgaaaaat ctacttcaga
     1621 tgtactgaaa caatatcaag aactagggtt tgatttgaca aaacctatga taatagagtt
     1681 ttttatagat ggttctcaaa agaatctgca atcaatagaa agccaactca tttcttatcg
     1741 gcaagatttt caaatcagta ttgaacaaga tgaattcgga gaaatgtgga cttgctattg
     1801 ttccgtcaat atcgtccctt cccttgaaaa cattttagct attgaaacta cgttatttgg
     1861 tattgcacaa aaaaatgatt gtaattatga aggttttggt tcgtatggga actaacaaaa
     1921 agtgtaggtt gggttgaaaa aaccatattc ccattaaaga ttaggggatg tctatggaaa
     1981 taaaattcaa tactttgggg gttatcctga atggagtcaa tcctgaagaa aaattcataa
     2041 aaattattga tgaccaggaa aataccggtg gatttctgat actgttatct tcaaatgaca
     2101 aattctcatc atttgacagt tacgatgact gggtcgaaaa cttagaaata ttaaaagaat
     2161 acctgcaaga atctcattgg ataataaagt gggtcggata gtcggcaaac gtagtccgtt
     2221 tgaaacgctt ttacttttca gacggacttt ctaccaatgg gaacaaggcg ttttgtctca
     2281 atccggttta tgattattcg atacagtgat gggaagctaa gaaaaatatg attaatcata
     2341 ttgaaaaata tataggcact atttctcatg gctcgaaaag cgattcggga agtcagtaca
     2401 aattaaatat cgcagctatt ccctcttccc ctaacaggga tttaaaaact tatattactt
     2461 tagggttaag caaacatgat ctgaattata aaagcagatt tgaaatactt tttgtatgtt
     2521 ctttaaaata tgatgaaaat cagattttcc catttttaag atggttagca gaaaccatca
     2581 ttgagaataa aaaaaatcct tcttcggggg caggttgttt atttgcctag aagcattgtt
     2641 aattcaacaa aaatggatgc actgtatgtt tccgccccat tttatttcga cgatgatttc
     2701 caagtctgtt atggcgaaca ctacaatatt gttttccctt tgcttgtccc cttgtataaa
     2761 caggaagccg aattggtgga aaaaaagggt tggaatgctt ttgagcagtt cttgttggat
     2821 aatgaagttg gcaacctttc ggatatgaat aggaaaccgt ttgtttggta agggctttct
     2881 taaatttgat taaaggtcgt ctgaaaccgt gtttaacttt cagacaacct ttccttcatt
     2941 tgaaacag
//
LOCUS       DAXDZG010000118         2921 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXDZG010000118 DAXDZG010000000
VERSION     DAXDZG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.118"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..173
                     /locus_tag="ACFB78_002008"
     CDS             <1..173
                     /locus_tag="ACFB78_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC1269919.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB78_002009"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB78_002009"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB78_002010"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB78_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFB78_002011"
     CDS             2263..2631
                     /locus_tag="ACFB78_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269920.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..>2921
                     /locus_tag="ACFB78_002012"
     CDS             2638..>2921
                     /locus_tag="ACFB78_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFC1269921.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNEQAWAE
                     NYLTAQGKALHERVDSDEPETRKGVRFERTVHVSVEKPGISGILDLVEADT"
BASE COUNT          812 a          699 c          749 g          661 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgctgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcggcga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aggcgcttgg cattgagtcg gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 tattaccgct tggatgtgta aaaacttctt tgacatccgc accttcggcg ccgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacccgcatg gcggtaacca atgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgcgc tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctccgccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacgccg tgaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgaacaggcg
     2761 tgggcggaga actatttgac cgcgcaaggc aaagcgttac atgagcgggt ggattcggac
     2821 gagccggaaa cgcgcaaggg cgtacgtttt gagcggacgg tacacgtttc ggtagaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata c
//
LOCUS       DAXDZG010000119         2758 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXDZG010000119 DAXDZG010000000
VERSION     DAXDZG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.119"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..603
                     /locus_tag="ACFB78_002013"
     CDS             <1..603
                     /locus_tag="ACFB78_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1269922.1"
                     /translation="AKNHQILSDEPSDFALRTVRFCAKNHQILSDEPSDFDGSLYVEP
                     SVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPETAPAAKAKKTGRHETELSLLA
                     DYGITGQVAADFLQVRKAKRQPLTETAMRLIAADAEKCGMTALQAAEYAIASGWGSFR
                     ADWLQNKTFGRSGNRGGPTHNQTAAVPDAGSYGDMPTTDF"
     gene            617..1399
                     /locus_tag="ACFB78_002014"
     CDS             617..1399
                     /locus_tag="ACFB78_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1269923.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGVFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLM
                     PMLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESYRA"
     gene            1469..1675
                     /locus_tag="ACFB78_002015"
     CDS             1469..1675
                     /locus_tag="ACFB78_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269924.1"
                     /translation="MREGAECGRAGEALPANQSLRRRGVSDGIGGGSRKKDGGAWGIS
                     PRNAPNLSGKAGKTLWEYPSLPEI"
     gene            1714..2208
                     /locus_tag="ACFB78_002016"
     CDS             1714..2208
                     /locus_tag="ACFB78_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFC1269925.1"
                     /translation="MTVQNTQTETVRTEAAPQQGGNTNPGYYKNRAFECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQRADAPKFKKLKCRRYEKMYAGLKDCGF
                     DGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETA
                     GGGI"
     gene            2385..2534
                     /locus_tag="ACFB78_002017"
     CDS             2385..2534
                     /locus_tag="ACFB78_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269926.1"
                     /translation="MDTLLSIITALSFAGAATLAVWLLVEAADAVLRRKRDGKGEDDF
                     DGFGY"
     gene            2562..>2758
                     /locus_tag="ACFB78_002018"
     CDS             2562..>2758
                     /locus_tag="ACFB78_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269927.1"
                     /translation="MAEEMRTCKACGGTKPLEKGFNAVPRKEGGVYYYKSCKTCRNKA
                     VRQKRAEKRAAAGAGAMTAARL"
BASE COUNT          643 a          772 c          910 g          433 t
ORIGIN      
        1 gctaagaacc atcagatttt gagcgatgaa ccatcagatt ttgcgctaag aaccgtcaga
       61 ttttgcgcta agaaccatca gattttgagc gatgaaccat cagattttga cggttcctta
      121 tatgtagaac cgtcagtaga accgtcagta gaaccgtcag tagaaccgtc aggatcaaat
      181 gcgcgcggcg cgcgcgcccc tgccggaccg caccctgcga aaccgcaaac ggcgcctccc
      241 gaaaccgcac cggcggcgaa ggcgaaaaaa accggcaggc acgaaaccga gctttcgctg
      301 cttgccgact acggcatcac ggggcaggtg gcggcggact tcctgcaagt ccgcaaggca
      361 aaacggcagc cgctgacgga aacggcaatg cgcctgattg ccgccgatgc ggagaaatgc
      421 gggatgacgg cgctgcaggc ggcggagtac gccatcgcca gcggctgggg cagcttccgc
      481 gccgactggc tgcaaaacaa aactttcggc aggtccggaa accgcggcgg cccgacgcac
      541 aaccaaaccg ccgccgtgcc ggatgcggga agctacggcg atatgccgac gacggatttt
      601 tgaggggggt tcggatatgg ctttgaggaa cgcgtctgat ttcttggggg cttacggcgg
      661 cggcgtgcgg gtcgagcgga ggcaatgcgc ggaacacggc ggatacgcgg cgaaaagcgt
      721 tttgcgcggc gtgtggacgg gctgcccggc ctgccggaag ctggaggcgg cggacgaaat
      781 ggcggcatac gcggaaacgc tgcgccgcgg ggcgatgcgc gacgcgctgg aaaaacgcat
      841 cgggcgttcg ggcatcgccc cgcggttcag aaactgccgg attgaaaact acgccgtcag
      901 cgattcgatc ccggggatgg cgagggcgaa ggcggccgcc gccgagtatg cggcaaactt
      961 cgccgatgtg ttgcagacgg ggcggagcat gattttttcg ggcaggaggg gcacgggcaa
     1021 aaaccacctt gcctgcggca tcgcccgcga agtcatcgcc gccggcaaaa gcgcgctggt
     1081 catcacggtg ggcgatatgc tgcggacggt caaggacagt ttcggcggcg gcggcggcga
     1141 ggcgggggcg gtcggggttt tcgtgaagcc cgatttgctg gtgctggacg agttcggcgc
     1201 gggcagtctg tcggaaacgg acgggcggat tttgttttcc gtcgtcaacg cccgctatga
     1261 gcggctgatg ccgatgctgg tgctgaccaa cctgacggcg gaagccttcc gcgaaaacac
     1321 cgacgcgcgg atcagggaca ggctgcggga cggcggcggc aagctgattc cgttcgactg
     1381 ggagagctac cgtgcgtgaa acctgctttt tctgcaaaca cgcggatttc aaaaccaaca
     1441 ccggcacgcc ggtgcgcggt tttgcgaaat gcgcgaaggc gcggaatgcg gaagagcggg
     1501 cgaagcatta cccgcgaacc aatccctgcg ccgccggggc gtttcagacg gcatcggggg
     1561 cggcagtcgc aaaaaggacg gcggtgcttg gggaatatcc ccccgcaatg cgccgaattt
     1621 gagcgggaag gcgggtaaaa cgctttggga atatcccagc ctacccgaga tttaaaaaac
     1681 gcgttaaaac gcaaatttga aaggaaatac ggaatgacgg tgcaaaacac gcaaaccgaa
     1741 accgtccgga cggaagccgc gccgcaacaa ggcggcaata ccaacccggg ctattacaaa
     1801 aaccgcgcct tcgagtgcgt cgggtttgcg caatacctca acttcaacct cggcaacgcc
     1861 ttcaaataca tctggcggca caaggaaaaa ggcgggcgcg aagacttgga aaaagccctg
     1921 cggtacttgg aacgccaacg cgccgacgcg ccgaagttca agaaactcaa atgccgccgc
     1981 tatgaaaaaa tgtacgccgg tctgaaagat tgcgggttcg acggcggcac ggaggccgcg
     2041 ctgcttgccg tcatctccgc cgcttattac atccgcgacg gcgaagacaa ttttgcgtgg
     2101 gcggccgcct gtgtcgaaga tttgttggaa aaaatgccgc ctgaagcggg gcgggccccg
     2161 caccctgaaa gcccgatgcc gcctgaaacg gcgggcggag gcatttgacc cgccaacccg
     2221 accgccgcca ttcccgcgaa agcgggaatc cggaatcccg gactttcaga taatctttga
     2281 atattgctgt tgttccaagg tccggattcc cgcctgcgcg ggaatgacgg aggcggcggg
     2341 aatccgaccc cgacccataa aaccgaccga aaggaaataa aacaatggat accctgttaa
     2401 gcatcatcac cgcgctgtcg tttgccgggg cggcgacgtt ggcggtatgg cttttggtgg
     2461 aagccgccga cgcggttttg cgccgcaagc gcgacggcaa aggcgaagac gacttcgacg
     2521 gcttcggata ttaagcaacc gaaacaaaag gaaaaatcaa aatggcggaa gaaatgcgca
     2581 cctgcaaggc ctgcggcgga accaagccgt tggagaaagg gtttaatgcc gtcccgcgca
     2641 aggaaggggg ggtctattat tacaaatcgt gcaaaacctg ccgcaacaag gcagtccggc
     2701 aaaagcgcgc ggaaaaacgc gcggcggcgg gagccggcgc gatgacggcg gcaaggct
//
LOCUS       DAXDZG010000120         2735 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXDZG010000120 DAXDZG010000000
VERSION     DAXDZG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.120"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..519)
                     /locus_tag="ACFB78_002019"
     rRNA            complement(<1..519)
                     /locus_tag="ACFB78_002019"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(866..941)
                     /locus_tag="ACFB78_002020"
     tRNA            complement(866..941)
                     /locus_tag="ACFB78_002020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Ala,seq:tgc)
     gene            complement(947..1023)
                     /locus_tag="ACFB78_002021"
     tRNA            complement(947..1023)
                     /locus_tag="ACFB78_002021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(987..989),aa:Ile,seq:gat)
     gene            complement(1124..2664)
                     /locus_tag="ACFB78_002022"
     rRNA            complement(1124..2664)
                     /locus_tag="ACFB78_002022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          574 a          809 c          583 g          769 t
ORIGIN      
        1 aggtttcagg ttctgtttca ctcccctccc ggggttcttt tcgcctttcc ctcacggtac
       61 tggttcacta tcggtcgatg atgagtattt agccttggag gatggtcccc ccatattcag
      121 acaggatttc acgtgtcccg ccctactttt cgtacgctta gtaccgctgt tgagatttcg
      181 aatacgggac tgtcacccgc tatggtcaag cttcccagct tgttcttcta tctcgacagt
      241 tattacgtac aggctcctcc gcgttcgctc gccactactt gcggaatctc ggttgatttc
      301 ttttcctccg ggtacttaga tggttcagtt ctccgggttc gcttctctaa gcctatgtat
      361 tcaacttagg atactgcaca gaatgcagtg ggtttcccca ttcggacatc gcggaatcat
      421 agctttattg ccagctcccc cgcgcttttc gcaggcttac acgtccttcg tcgcctatca
      481 tcgccaaggc atccgcctga tgcacttatt cacttgactc tatcatttca agaacttctt
      541 tgacttcgtt tacctacccg ttgactaagt aagcaaactt gaaatcccta ctttgataaa
      601 gcttactgct ttgttgtgtc ttaatcctgc cttttgtgtt tcaggattaa gtcgatacaa
      661 tcatcaccca aatactatgt ttgttttctt ttctcttgcg agaggttttt atcctttgca
      721 aagaataaaa aatcaaaaca aactcattgt ctttgtttgt tgatttcggc tttccaattt
      781 gttaaagatc gatgcgtcgt tattctactt cgcaaatcaa aataagctgc taaaaacagc
      841 aaacttgctt tcatttgtaa agttttggtg gaggcaaacg ggatcgaacc gatgaccccc
      901 tgcttgcaaa gcaggtgctc taccaactga gctatgcccc cgttcttggt gggtctggga
      961 ggacttgaac ctccgacccc acgcttatca agcgtgtgct ctaaccagct gagctacaaa
     1021 cccggtttcc cttcttaagc gaaccttgcc ttcactcaag cttcttccgc atcttttcag
     1081 tttaccgata agtgtgaatg cctaaagccc cttctttctc tagaaaggag gtgatccagc
     1141 cgcaggttcc cctacggcta ccttgttacg acttcacccc agtcatgaag cataccgtgg
     1201 taagcggact ccttgcggtt accctaccta cttctggtat cccccactcc catggtgtga
     1261 cgggcggtgt gtacaagacc cgggaacgta ttcaccgcag tatgctgacc tgcgattact
     1321 agcgattccg acttcatgca ctcgagttgc agagtgcaat ccggactacg atcggttttg
     1381 tgagattggc tccgcctcgc ggcttggcta ccctctgtac cgaccattgt atgacgtgtg
     1441 aagccctggt cataagggcc atgaggactt gacgtcatcc ccaccttcct ccggcttgtc
     1501 accggcagtc tcattagagt gcccaaccga atgatggcaa ctaatgacaa gggttgcgct
     1561 cgttgcggga cttaacccaa catctcacga cacgagctga cgacagccat gcagcacctg
     1621 tgttacggct cccgaaggca ctcctccgtc tccggaggat tccgcacatg tcaaaaccag
     1681 gtaaggttct tcgcgttgca tcgaattaat ccacatcatc caccgcttgt gcgggtcccc
     1741 gtcaattcct ttgagtttta atcttgcgac cgtactcccc aggcggtcaa tttcacgcgt
     1801 tagctacgct accaagcaat caagttgccc aacagctaat tgacatcgtt tagggcgtgg
     1861 actaccaggg tatctaatcc tgtttgctac ccacgctttc ggacatgaac gtcagtgtta
     1921 tcccaggagg ctgccttcgc catcggtatt cctccacatc tctacgcatt tcactgctac
     1981 acgtggaatt ccacctccct ctgacacact cgagtcaccc agttcagaac gcagttcccg
     2041 ggttgagccc ggggatttca catcctgctt aagtaaccgt ctgcgcccgc tttacgccca
     2101 gtaattccga ttaacgctcg caccctacgt attaccgcgg ctgctggcac gtagttagcc
     2161 ggtgcttatt cttcaggtac cgtcatcggc cgccgatatt ggcaacggcc ttttcttccc
     2221 tgacaaaagt cctttacaac ccgaaggcct tcttcagaca cgcggcatgg ctggatcagg
     2281 cttgcgccca ttgtccaaaa ttccccactg ctgcctcccg taggagtctg ggccgtgtct
     2341 cagtcccagt gtggcggatc atcctctcag acccgctact gatcgtcgcc ttggtgggcc
     2401 tttaccccgc caaccagcta atcagatatc ggccgctcgg atagcgcaag gcccgaaggt
     2461 cccctgcttt ccctctcaag acgtatgcgg tattagctga tctttcgatc agttatcccc
     2521 cgctacccgg tacgttccga tatgttactc acccgttcgc cactcgccac ccgagaagca
     2581 agcttccctg tgctgccgtc cgacttgcat gtgtaaagca tgccgccagc gttcaatctg
     2641 agccaggatc aaactcttat gttcaatctc taacttttta acttctggtc tgcttcaaag
     2701 aaaccgacag gacaatgttc aaaacatcat ctcgt
//
LOCUS       DAXDZG010000121         2682 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXDZG010000121 DAXDZG010000000
VERSION     DAXDZG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.121"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            277..1074
                     /gene="truA"
                     /locus_tag="ACFB78_002023"
     CDS             277..1074
                     /gene="truA"
                     /locus_tag="ACFB78_002023"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC1269928.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDITAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRTDLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
     gene            1591..2637
                     /gene="porB"
                     /locus_tag="ACFB78_002024"
     CDS             1591..2637
                     /gene="porB"
                     /locus_tag="ACFB78_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IB"
                     /protein_id="HFC1269929.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGAIKAGVQTYRSVEHTDGKV
                     SKVETGSEIADFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTKDNVNAWESGKFTGDVLEISRMAKREHRYLSVRYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYRNGGFFAQYAGLFQRYGEGTKKIEYDNQFYSV
                     PSLFVEKLQVHRLVGGYDNNALYASVAAQQQDAKLYGTWRANSHNSQTEVAATAAYRF
                     GNLTPRVSYAHGFKGSVHSADYDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADK
                     VVSTASAVVLRHKF"
BASE COUNT          689 a          811 c          639 g          543 t
ORIGIN      
        1 cgtcattccc gcgcaggcgg gaatccagac ctgtcggtgc ggaaacttat cggataaaac
       61 ggtttcttag attccacatc ctagattccc gcctgcgcgg gaatgacggt tcgggcactc
      121 ctgacagggt gaattcacag gatagcgatt cgtagcaact gcatccaacc ccccgaacaa
      181 ctccccaaac aacgccgcgc gccccgggcg tttgccgttt ccctgcaaaa tctgcgatac
      241 aatgcagtct gaacatttat ccgaatccca aacccgatgg ataccgcaca aaaacaacgc
      301 tgggcaataa ccctatccta cgacggcagc cgcttttacg gctggcagaa acaggcgggc
      361 ggcgtaccga ccgttcaggc ggcattggaa accgcactcg cccgaatcgc aggagaatct
      421 gtcgccacca cagttgccgg caggaccgac accggcgtgc acgctgccgc ccaagtcgtc
      481 cactttgaca taactgccgc ccgtccccaa caggcatggg tgcgcggcgt aaatgcccac
      541 ctgcccgaag gcattgccgt tttgcacgcc cgacaggtcg cacccggatt tcacgcccgc
      601 ttcgacgcat acggacggca ctaccgctac ctgctcgaat ccgcccccgt ccgttccccc
      661 ctgctcaaaa acagggcagg ctggacacac ctcaaactcg acatcgaacc gatgcgccgg
      721 gctgccgcct tattgatcgg cgaacaagac ttctccagct tccgcgccgc cgggtgccag
      781 gcaaaatccc ccgtcaaaac catctaccgc accgacctta cccaaagcgc aggactgatc
      841 cgcctcgatt tgcacggcaa cgcctttttg caccatatgg tacgcaacat catgggcgcg
      901 ctcgtttatg tcggcagcgg caggctcagc gtcgaaggct tcgccgcact gattcaagaa
      961 cgcagccgcc tcaaagcccc accgaccttc atgcccgacg ggctttacct gaccggcgtc
     1021 gactatcccg gggcatacgg catcgtccgc ccccaaatcc ccgaatggct ttaaaacatt
     1081 cttgtcgcgg agattttgaa atcggacaaa ctgtcaggca atctttttca atgttgacac
     1141 tatagtggat taacaaaaat caggacaagg cggcgggccg cagacagtac aaatggtacg
     1201 gaaccggttc gcccggtgct tgggcgcctt agggaaccgt tccctttggg ccggggcggg
     1261 gcaacgccgt accggttttt gttaatccgc tataaccccc ggtttgcaat gccctccaat
     1321 accctcccga gtaagtgttg taaaaatgca aatcttaaaa attttaaata accatatgtt
     1381 acaaaacaaa aaatacccgt aatatctcta tccgtccttc aaaatgcaca tcggattcca
     1441 cacaaaaaca ggcagaagtt tgttttttca gacaggaaca tctatagttt cagacatgta
     1501 accgccgagc ccctcggcgg taaatgcaaa gctaagcggc ttggaaagcc cggcctgctt
     1561 aaatttctta accaaaaaag gaatacagca atgaaaaaat ccctgattgc cctgactttg
     1621 gcagcccttc ctgttgcggc aacggccgat gtcaccctgt acggtgccat caaagccggc
     1681 gtacaaactt accgttctgt agaacataca gacggcaagg taagtaaagt ggaaaccggc
     1741 agcgaaatcg ccgacttcgg ttcaaaaatc ggcttcaaag gccaagaaga cctcggcaac
     1801 ggtctgaagg ccgtttggca gttggaacaa ggtgcctccg tcgccggcac taacaccggc
     1861 tggggcaaca aacaatcctt cgtcggcttg aagggcggct tcggtaccat ccgcgccggt
     1921 agcctgaaca gccccctgaa aaacaccaag gacaacgtca atgcttggga atccggcaaa
     1981 tttaccggcg atgtgctgga aatcagcaga atggccaaac gggaacaccg ctacctgtcc
     2041 gtacgctacg attctcccga atttgccggc ttcagcggca gcgtacaata cgcacctaaa
     2101 gataattcag gctcaaacgg cgaatcttac cacgttggct tgaactaccg aaacggcggc
     2161 ttcttcgcac aatacgccgg cttgttccaa agatacggcg aaggcactaa aaaaatcgaa
     2221 tacgataatc aattttatag tgtccccagc ctgtttgttg aaaaactgca agttcaccgt
     2281 ttggtcggcg gttacgacaa taatgccctg tacgcctccg tagccgcaca acaacaagat
     2341 gccaaattgt atggaacatg gcgtgctaat tcgcacaact ctcaaaccga agttgctgct
     2401 accgcggcat accgtttcgg caacttaacg ccccgcgttt cttacgccca cggcttcaaa
     2461 ggctctgttc atagtgcaga ctacgacaat acttatgacc aagtggttgt cggtgcggaa
     2521 tacgacttct ccaaacgcac ttctgccttg gtttctgccg gctggttgca agaaggcaaa
     2581 ggcgcagaca aagtcgtatc gactgccagc gccgtcgttc tgcgccacaa attctaatct
     2641 gcaaagattg gtatcaacaa aaagcctgtc gccagacagg ct
//
LOCUS       DAXDZG010000122         2678 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXDZG010000122 DAXDZG010000000
VERSION     DAXDZG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.122"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(234..647)
                     /locus_tag="ACFB78_002025"
     CDS             complement(234..647)
                     /locus_tag="ACFB78_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269930.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(644..1105)
                     /locus_tag="ACFB78_002026"
     CDS             complement(644..1105)
                     /locus_tag="ACFB78_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1269931.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1122..1559)
                     /locus_tag="ACFB78_002027"
     CDS             complement(1122..1559)
                     /locus_tag="ACFB78_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269932.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(1672..2388)
                     /locus_tag="ACFB78_002028"
     CDS             complement(1672..2388)
                     /locus_tag="ACFB78_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC1269933.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            2463..>2678
                     /locus_tag="ACFB78_002029"
     CDS             2463..>2678
                     /locus_tag="ACFB78_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC1269934.1"
                     /translation="MSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRCVQ
                     IEKLTGGAVNRKDLRPDDWHLIWPELAG"
BASE COUNT          645 a          575 c          639 g          819 t
ORIGIN      
        1 gccaaaccga tttattctgc tgattaattc gttcatcccg tttccttcaa gttgttgttt
       61 gtttcgatgg gtgtattata actattacct atatttaatc aataggcaat agttatattt
      121 tcatccatat atacttattg aattgatttt tagaagaaaa aagtttgaaa aaaaaccgcc
      181 cgaatggcgg tgtatcatga aaaagaaaac cgccctaggg cggttcggta ttgttatgag
      241 tggtaaggaa gccaagaaaa agacaaagga cggctctcgg agagaatatg gttgtgtgtt
      301 gtcattctta cataaatgcg gtcgttgtct gaaacgggta gtccgctgat tgtttggtgt
      361 agcatgaatg acacgttttc ttctgtatca ctgccgtcat atgatggaat ctcaagtgca
      421 gagattacat catcgttgcg gacgatttcc agaaaaattt gaccggtttt catagtctgt
      481 atatccatgc cttcggcgga aaatgaaaca gacaggcaca ggcggcctag aatggcctgc
      541 ggtgtcggaa tatggcatat atcggggaat atacctatca ggctgtattt gtcattttcc
      601 gcatggcgga taatttcatc gcaatagtgg acatttaagt gaatcatgcc tgctccatat
      661 attcgtaccg ccgttcgaac gctgcgcgaa cttcaagcgg ggaaacgcct aatgcgtttg
      721 ccaatttttg tacagtctta tcttgcaggg attgtttgct gttttcgatg cgggacagat
      781 agggttgagg caatcctgcg gcggttgcca attcggactg ggtaaacccc tttttcatcc
      841 ttaagctgac aaatgtttcc ccgcctgctt tgagggctat tttatcggca attcttgctg
      901 ccgccctgtc catcgctgct ttgcggcgcg gatttttccg cacatgctca atatgctctg
      961 cggcaggggt agtgttaggg cttatcggtt tgacgcattt aatctgcccg tttatccgca
     1021 ggcattttat gtgggtgacg gggatggcgg atgtaggtgt tgatactgtg caggcggcca
     1081 cggcaacagt gagtgttgca cccattttga accatgtgcc gttagttgga gtacagttct
     1141 tcatagtctt tgataatccg ttttgtgatt gggtgttctg cctgatagtc aaattcgtct
     1201 gtcttcttgt ttacaacagc caatatatct atgcggcgga tatacgtgcc gtttggctgt
     1261 ttttcatggc atggtgcgta aattattctg tatccggcgg cttcttcgct gtcaaatcgg
     1321 acgcgtaaaa ctttgatgtc ttttccccat aatgacaata tgggtttcac ttccaagtca
     1381 atcgggccta tgggttcgcc atattccctg aagtatctgt ttttgtataa gccgtcaaaa
     1441 atagcagagt ccgcctgaat catcgcaatg acattctcga gataaccgac cgcttcttcg
     1501 tctttttcaa agaggcggtc taaatcctgc tcggcgttaa agtggacggt cagctccatg
     1561 agtgcccttt ttatatatat attatatctt tttagttata ccgcgcaata tgcggtaata
     1621 aatcagttgt tcaagtattt taatccggca cgctccacca gaagacgcgg tctagaactg
     1681 gctcaaacgc catgcgccgc gtatgcgtcc gatgatgcgt acggcgttta aatcttcgcc
     1741 gcgcacggtt tcggttcggt atgagctgtt gtcgctgatg atcatcaggc cgccgccgac
     1801 ggtggattgc agccgcttgg ccttaaggcc gtctatatac caaagcaggt agaggccgtc
     1861 gccatcgaag gcttcgacgg cggtatcaac gaacattacg tcgccgtttt cgatggtggg
     1921 ctccatgctg tcgccacggg ctgtaatgac ttggattttg ttgaggtttc cgcccagttt
     1981 ctcccgcgcc catgcggcag cgacggttac ataatccaca acctcgatat agtggtcgtt
     2041 aatcgtgcct gcgccgcagg tcgcttcggc atttaagcgg gggaaacgta tgctactttg
     2101 ttcattcgat tttaagactc tgaatgtttc aatgccgaaa tggcttggcg ttactacgtc
     2161 tgagaaataa tcaattaatt tatctagatg ttttttatct attcgtccat tttttatcca
     2221 acctgaaacg cttggctgtt tcaccccaaa atgatcggca acctcctttt gactgacatt
     2281 ttttctctta atcgcttctg atattgcttg tcctaactgt tcgcctgaaa acattttgat
     2341 ctccgaatta attgcgcata agcattggta atcgataata aaaggcataa cctataaaag
     2401 gcaatagttg tatttaatat aagtattagc tataatgaag ctatttaatt gagtaactgg
     2461 ctatgagtat ccaaaaagca gttgattatt ttggtaatga atcccgactt gcacgggcga
     2521 tcggagttaa acaaccgacg gtgtgggctt ggaataaaaa aggaacgccg cccccgatca
     2581 ttcggtgcgt gcagattgaa aaattaaccg gaggcgcagt gaatcgaaaa gacttacgtc
     2641 ctgatgactg gcatctaatc tggccggaat tggcaggc
//
LOCUS       DAXDZG010000123         2644 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXDZG010000123 DAXDZG010000000
VERSION     DAXDZG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.123"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..620
                     /locus_tag="ACFB78_002030"
     CDS             <1..620
                     /locus_tag="ACFB78_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC1269935.1"
                     /translation="QALDEGVPYIAVFEDDVLLGEGAEQFLAEDTWLQERFDPDSAFV
                     VRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISRKAMRFFLDRFAVLPPE
                     RLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQNSALGSLIEHDRRLNRK
                     QQRRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIVPFQ"
     gene            632..1554
                     /locus_tag="ACFB78_002031"
                     /pseudo
     CDS             632..1554
                     /locus_tag="ACFB78_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            1614..2627
                     /locus_tag="ACFB78_002032"
     CDS             1614..2627
                     /locus_tag="ACFB78_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1269936.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARHFQEQDGRIRIISNPRNLGFIASLNIGLDELAKSGGGEYIARTDADDIAAP
                     GWIEKIVGEMEKDRSIIAMGAWLEVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVF
                     PFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRF
                     HQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTAYALYEKAL
                     SGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKY
                     RSY"
BASE COUNT          662 a          671 c          733 g          578 t
ORIGIN      
        1 aacaggcgtt ggacgaaggc gtaccgtata tcgccgtatt tgaagatgat gtcttactcg
       61 gcgaaggcgc ggagcagttc cttgccgaag atacttggct gcaagaacgc tttgaccccg
      121 attccgcctt tgtcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
      181 gcgtggcgga ctacggcggg cgcgcctttc cgcttttgga aagcgaacac tgcgggacgg
      241 cgggctatat tatttcccga aaggcgatgc gttttttctt ggacaggttt gccgttttgc
      301 cgcccgaacg cctgcaccct gtcgatttga tgatgttcgg caaccctgac gacagggaag
      361 gaatgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
      421 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca tgaccgccgc ctgaaccgca
      481 aacagcaaag gcgcgattcc cccgccaaca cattcaaaca ccgcctgatc cgcgccttga
      541 ccaaaatcgg cagggaaagg gaaaaacgcc ggaaaaggcg cgaacagaca atcggcaaga
      601 ttattgtgcc tttccaataa aaggagaaaa gatggacatc gtatttgcgg cagacgacaa
      661 ctatgccgcc tacctttgcg ttgcggcaaa aagcgtggaa gcggcccatc ccgatacgga
      721 aatcaggttc cacgtcctcg atgccggcat cagtgaggaa aaccgggcgg cggttgccgc
      781 caatttgcgg gggggggggg taatatccgc tttatagacg taaaccccga agatttcgcc
      841 ggcttcccct taaacatcag gcacatttcc attacgactt atgcccgcct gaaattgggc
      901 gaatacattg ccgattgcga caaagtcctg tatctggata cggacgtatt ggtcagggac
      961 ggcctgaagc ccttatggga taccgatttg ggcggtaact gggtcggcgc gtgcatcgat
     1021 ttgtttgtcg aaaggcagga aggatacaaa caaaaaatcg gtatggcgga cggagaatat
     1081 tatttcaatg ccggcgtatt gctgatcaac ctgaaaaagt ggcggcggca cgatattttc
     1141 aaaatgtcct gcgaatgggt ggaacaatac aaggacgtga tgcaatatca ggatcaggac
     1201 attttgaacg ggctgtttaa aggcggggtg tgttatgcga acagccgttt caactttatg
     1261 ccgaccaatt atgcctttat ggcgaacggg tttgcgtccc gccataccga cccgctttac
     1321 ctcgaccgta ccaatacggc gatgcccgtc gccgtcagcc attattgcgg ctcggcaaag
     1381 ccgtggcaca gggactgcac cgtttggggt gcggaacgtt tcacagagtt ggccggcagc
     1441 ctgacgaccg ttcccgaaga atggcgcggc aaacttgccg tcccgccgac aaagcatatg
     1501 cttcaaagat ggcgcaaaaa gctgtctgcc agattcttac gcaagattta ttgacggggc
     1561 aggccgtctg aagccttcag acggcatcgg acgtatcgga aaggagaaac ggattgcagc
     1621 ctttagtcag cgtattgatt tgcgcctaca acgtagaaaa atattttgcc caatcgctgt
     1681 ccgccgtagt ggggcagact tggcgcaact tggatatttt gattgtcgat gacggctcga
     1741 cggacggcac gcccgccatt gcccggcatt tccaagaaca ggacggcagg atcaggataa
     1801 tttccaatcc ccgcaatttg ggctttatcg cctctttaaa catcgggctg gacgaattgg
     1861 caaagtcggg ggggggggaa tatattgcgc gcaccgatgc cgacgatatt gccgcccccg
     1921 gctggattga gaaaatcgtg ggcgagatgg aaaaagaccg cagcatcatt gcgatgggcg
     1981 cgtggttgga agttttgtcg gaagaaaaca ataaaagcgt gcttgccgcc attgcccgaa
     2041 acggcgcaat ttgggacaaa ccgacccggc atgaagacat tgtcgccgtt ttccctttcg
     2101 gcaaccccat acacaacaac acgatgatta tgaggcgcag cgtcattgac ggcggtttgc
     2161 ggttcgatcc agcctatatc cacgccgaag actataagtt ttggtacgaa gccggcaaac
     2221 tgggcaggct ggcttattat cccgaagcct tggtcaaata ccgcttccat caagaccaga
     2281 cttcttccaa atacaacctg caacagcgca ggacggcgtg gaaaatcaaa gaagaaatca
     2341 gggcggggta ttggaaggcg gcaggcatag ccgtcggggc ggactgcctg aattacgggc
     2401 ttttgaaatc aacggcatat gcgttgtacg aaaaagcctt gtccggacag gatatcggat
     2461 gcctccgcct gttcctgtac gaatatttct tgtcgttgga aaagtattct ttgaccgatt
     2521 tgctggattt cttgacagac cgcgtgatga ggaagctgtt tgccgcaccg caatatagga
     2581 aaatcctgaa aaaaatgtta cgcccttgga aataccgcag ctattgaaac cgaacaggat
     2641 aaat
//
LOCUS       DAXDZG010000124         2625 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXDZG010000124 DAXDZG010000000
VERSION     DAXDZG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.124"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(100..489)
                     /locus_tag="ACFB78_002033"
     CDS             complement(100..489)
                     /locus_tag="ACFB78_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC1269937.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(652..1031)
                     /locus_tag="ACFB78_002034"
                     /pseudo
     CDS             complement(652..1031)
                     /locus_tag="ACFB78_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1085..2218)
                     /gene="carA"
                     /locus_tag="ACFB78_002035"
     CDS             complement(1085..2218)
                     /gene="carA"
                     /locus_tag="ACFB78_002035"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC1269938.1"
                     /translation="MNTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNAGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          541 a          654 c          741 g          689 t
ORIGIN      
        1 ttcaggttgc ttttttgctt ttcagacgac ctttttaaac gcttaattca gttaatttta
       61 ccttattttt ttcaaaatct aatcaaaatc aaccgtttgc taccgtgctg gctgcttttc
      121 caattcctgc aatacgcgca ggatttccgc cagtacatcg tttgtctgct gcaaaatttc
      181 gtgattccaa aaacgcagca cggtaaagcc caagctgttg agatataccg tccgcgcgtg
      241 gtcgtatacg gcttgttccg cgtgctgccc gccgtctgct tcgacaatca gcttgggcgt
      301 tacgcacata aaatcaacaa tataattccc catcggctgc tgtcggcgga atttatagcc
      361 gttcagacgg cctgcccgca ggtgttgcca caattttgct tccgcctcgc tcatttcttg
      421 acgcatggct ttggcgcgtt ggcgcagggc ggggttttcg gcggtcaata gtttctcggg
      481 cgggttcatt tttgttatcg ggttgctgtt tgtgtaagcc ttacagcttg ttgccctctc
      541 tctaactctt tcccacaggg agagaggacg ggtcggctgt tggggttaaa ggggctgcaa
      601 ggaaaacagc ttgtgcaggc tgttttttat tttgcataat ccgtctgaaa attaataact
      661 gctgatttcc agcaggttgc catcgggatc gcgcaggtaa accgattgga ttttgcccat
      721 tgcgcctgtg cgcgctatga tgccgcttaa aggtttgatg ccgtgtacgg ataattcctg
      781 taaaaccgtt tccagtgggg cgtatcggtc agcaggcata aatccgctgt gccgcaggcg
      841 gcgtgttgcg cgttaggctg aatttccgca ccgcgcccgt gtaggttgat tttctgactg
      901 ccaaacaaca aagctttacg gttgttgcca aatgaaactt cttccatgcc caaaacttgt
      961 gtgtaaaacg cgatggttcg gtcaatgtcg gcaacagtca gtactagatg gtcgagtgcg
     1021 ctaattttca tttccagctt tctttttcag acggcagcag catactgttg ccgtctgaaa
     1081 atcattatgc ttgttttgca gccttcatat tgccaatgaa tttgtcgaac aaatagccga
     1141 catcttgcgg acccgggctg gcttcggggt gtccttggaa acagaacacg ggtttgtcgg
     1201 tcagctcgat gccttgcaag gtattgtcga acaaggattt gtgggtaatg cgtgcgttgg
     1261 cgggcagggt gtcggcatcg acggcgaaac cgtggttttg gctggtaatg acgactttgc
     1321 cgctgtccaa atcttgcaca ggatggttcg caccgtggtg gctgaagcgc attttcaggg
     1381 ttttggcgcc gatggcgagg ctgatgagct ggtgtcccaa gcagatgccg aaaatcggtt
     1441 tgccgctttc catcagtttt tgcacggctt cgatggcgta ggtgcaaggc tcggggtcgc
     1501 cggggccgtt ggacaggaac acgccgtcgg gattgagtgc caacacgtct tccgcgctcg
     1561 tttgtgcggg gacgacggtc aggcggcagc cgcgcgaggc gagcatacgc aggatgttgg
     1621 ttttcacgcc gaaatcgtag gcgacgacgt ggtaaggctg tttgtcaggg gtaacgaaac
     1681 ctttgcccaa ttcccattcg ccttccgtcc attcgtaagt ttccgtgcag gaaacttctt
     1741 ttgccaaatc tttgccgacc atgctgccga atgcggcgat gagttcttgc gctttttcaa
     1801 cggtggcatc cgcacctgtc agaatcgcgc cgccttgcgc gcctttttcg cgcaacagca
     1861 tggtcaggcg gcgggtgtcg atgtcggcga tggcgacggt ttcgttgcgt accaaatagt
     1921 cgtgcaggct ttcggaggcg cggaagttgc tgtgcaagag cggcaggtcg cggataatca
     1981 ggcctgcggc gtaaacgctg cggctttctt catcttcggc gttggtgccg gcattgccga
     2041 tgtgcggata ggtaagggta acgatttgtt tgcagtagga cggatcggtc aggatttcct
     2101 gatagccggt catcgaagta ttgaacacga cttcgccgga agccgaacct tcgtaaccga
     2161 ttgatgtgcc gtggaatacg ctgccgtcag cgaggacgag gagggcgggg gtgttcatga
     2221 tgggaatcct gtttttaata aaattcttga caatgccgtc tgaagggact tcggcagggg
     2281 gcatccggtc ggaaaaatcc ggtcaaaaaa aacacgccgc agaacaaaac cgcgtaacgt
     2341 gcttttcggg caagtgccta taccctgaaa gcagcatatt ttaagacgaa acatacccga
     2401 tttcaagcgc aaacgcggca ggcgggctga tatgttcgcg cgaaaacgga aatccggggc
     2461 ataaaaccgc ccgctccgcg ccccctatgc ccgttacgca tttccccacg tccgcccggc
     2521 gaaactatgg gaatacccga accgtcattc ccgcgcaggc gggaatccgg acctgtcggc
     2581 acggaaactt atcgggtaca acggtttctt gagattttac gtcct
//
LOCUS       DAXDZG010000125         2615 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXDZG010000125 DAXDZG010000000
VERSION     DAXDZG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.125"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(46..705)
                     /locus_tag="ACFB78_002036"
     CDS             complement(46..705)
                     /locus_tag="ACFB78_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC1269939.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
     gene            865..1080
                     /locus_tag="ACFB78_002037"
     CDS             865..1080
                     /locus_tag="ACFB78_002037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269940.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            1026..1569
                     /locus_tag="ACFB78_002038"
                     /pseudo
     CDS             1026..1569
                     /locus_tag="ACFB78_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1639..2569
                     /locus_tag="ACFB78_002039"
                     /pseudo
     CDS             1639..2569
                     /locus_tag="ACFB78_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          606 a          757 c          735 g          517 t
ORIGIN      
        1 aggcttcaga cggcattttt gccgcgtgcc ggatgcggaa accaatcagg cgtaatgtcg
       61 tgcaagaaaa ccgggcagtt cggacaaacc gtccaatacg gcgagatgcg gtgcgccaag
      121 gagctgttcg cgcgaatgtg cgccggtggc aacgccgact gccgccgcgc ctgcgtttgc
      181 cgccatatgc aggtcgtgcg ccgtatcgcc gacaaccaat gcctctttcg ggtcgagtcc
      241 cagttcgccg cagagtccga ataccatttc gggcgagggt ttggaggggt attcccccgc
      301 gcaggcggtg gcgagccaat agccgccggt ggcggtttgg ctgatggcgt tgtccaaacc
      361 cgcccgccct ttgcccgtgg cgacggcaag ccggaagcct tgcgctttga gcttgtccag
      421 acagggcggg gcatcgggaa agagtgtcat attgcggttg ttgggattga ggtaatgcgc
      481 ggaataagtg cgcgcgatgt cggcaacggc agcttcagaa ggcatttcga gcagggcgcg
      541 gatgatttcg ggcaggctgt atccgatcag gctgcggacg cgctccgctt cgggcggcgg
      601 aaaaccgcat tcggcgaagc tgcggcgcat ggtgtcgatg atgggttggg tcgtatcggc
      661 aagcgtgccg tcccagtcga agatgatgag tttgggcggg gtcatggcag gttggttgca
      721 gtaaaaaagc agattttatg cggaaaacgc agacgtgtcg cattttcgac aaaatttgtc
      781 ggctgagcga tatgtttttc cgaacaagcc gcgttgtgct ttattaaaat agaaccatta
      841 tcatttatac agatgggaca gtttatgtca gttttccgca tcaatatgac cgccgccacg
      901 gttttggcag cactctcgtc tccggttttt gccgcacaaa cggcggattt ggaaaccgtc
      961 catatcaaag ggcagcgttc gtacaacgcg attgccaccg agaaaaacgg cgattacagc
     1021 tcgttaaagg cagctatatg gatgaccgcc tcaatacccg cgtctccccc tgccgcctga
     1081 aagacaaaaa cgccgccgaa cccgaacaac cgcaacaccc gttacgccgc attgggcaaa
     1141 cgcgtgatgg aaggcgttga gaccgaaatc agcggtgcga ttacaccgaa atggcaaatc
     1201 catgcaggtt acagctatct gcacagccaa atcaaaaccg ccgccaatcc acgcgacgac
     1261 ggcatcttcc tgctggtgcc caaacacagc gcaaacctgt ggacgactta ccaagttacg
     1321 cccgggctga ccgtcggcgg cggcgtgaac gcgatgagcg gcattacttc atctgcaggg
     1381 atgcatgcag gcggttatgc cacgttcgat gcgatggcgg cataccgctt cacgcccaag
     1441 ctgaagctgc aaatcaatgc cgacaacatc ttcaaccgcc attactacgc ccgcgtcggc
     1501 ggcacgaaca cctttaacat tcccggttcg gagcgcagcc tgacggcaaa cctgcgttac
     1561 agtttttaaa gaccaatatg ccgtctgaaa cggcagccgc agcataatca aaccacaaca
     1621 agctgcgcgg cataccctat gcgctcacaa ccggagtatg gcattgcgaa ggaaaacaga
     1681 ccgaaccggc aggcagaccg ctttgccggt tcggttttac cgcttgccgc cagtctgacc
     1741 cacaagccga acatcatgaa acccataccg accgacacat tccaacctgc catactgccc
     1801 caagcctttg aaaccgaaat caaatccacc tgcacggggc gaatctatcg gattcagacg
     1861 gcaacactcg gcgaaatgca gtctgaaggc tatcccgtcc tctttgtcct cgacggcgaa
     1921 gcctttttcc cgcgctgtac aacatcatgc agtcgctgat gaacaacccc gttacccgaa
     1981 gcaacgcccc ctgcctgatt gtcggtatcg gctacacgac aggcagtgtg cgcgacctgg
     2041 cgcagcgtgc cgccgactac acgccgccgc ttggagacaa cgccacagca gacgaacggc
     2101 ggcagttcgg acaggcagac cgcttcgccg actttatcga cagcgaactg accgcctttt
     2161 tagaaagccg ctacaccctt aaccgtaatg aaaccgccgt attcggacac tcgttcggcg
     2221 cactgttcgg actgtattcc ctgctttccc accgctgttt cagacggcat tggctcgtat
     2281 cccctcgatt tggtggcaca acaggcggat actcgacttt atgccgtctg aaaaccggct
     2341 ggacggcatc gatgcctgcc tcaacatcgg cgcactcgag cggagcagcg gttgcaaacg
     2401 cagggaagaa cgcgacatgg cagggcaggc cgaacaaatg gcggcagagt tggacaggcg
     2461 cgggaccgcc gtatttttcc gggaatatcc gaatgccgac cacggcaatg tcccgtttta
     2521 ctcgctgacc gactgcgtcg aatatttgag ggaagcgtgg caacggtagg gggaatcaaa
     2581 tatatgactg ctttgttttg catcggaaaa tatag
//
LOCUS       DAXDZG010000126         2562 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXDZG010000126 DAXDZG010000000
VERSION     DAXDZG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.126"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            111..395
                     /locus_tag="ACFB78_002040"
     CDS             111..395
                     /locus_tag="ACFB78_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC1269941.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            396..1583
                     /locus_tag="ACFB78_002041"
     CDS             396..1583
                     /locus_tag="ACFB78_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC1269942.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            1798..>2562
                     /locus_tag="ACFB78_002042"
     CDS             1798..>2562
                     /locus_tag="ACFB78_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC1269943.1"
                     /translation="MNIIGPDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKINDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKS"
BASE COUNT          898 a          544 c          576 g          544 t
ORIGIN      
        1 gcgagggggt gtccccacag cgctgccgcg ccgcgaatgc ggagcaatct ttcagattaa
       61 gaaacatttg tttaatgagg caaacgtgcc ttttaagaaa gggagagcaa atgaaattgt
      121 tggccgcatt gattccgctc ttgatgagcg tcgcaggccg tatattgact gcattaggct
      181 tgatggctgt tacctatgcg ggtgtagata gattggcagc ccattttcaa caggcgataa
      241 cccatagcat aacgggcgcg cctcaagcaa tgttacagct tttctatata agcggcggtg
      301 gtactgttct aaacattctt ttcggcgcaa tcgcctttat tctgtccttt aaacaaatga
      361 caaaactcgc aacctcaatc gggaagaaaa aataaatggc agaaatctgt ttgataaccg
      421 gcacgcccgg ttcagggaaa acattaaaaa tggtttccat gatggcaaac gatgaaatgt
      481 ttaagccaga tgaaaacggc gtacgccgta aagtatttac gaacatcaaa ggtttgaaga
      541 taccgcacac ccacatagaa acagacgcaa agaagctgcc gaaatcaacc gatgaacagc
      601 tttcggcgca tgatatgtat gaatggatca agaagcctga aaacgtcggc gcaatcgtta
      661 ttgtcgatga ggcgcaagac gtatggcccg cacgctccgc aggttcgaaa atccccgaaa
      721 acgtccaatg gctgaacaca cacaggcatc agggcataga tatatttgta ttgacacaag
      781 gtcctaaact cttagatcag aacttgcgaa cattggttaa aagacattac cacattgcgg
      841 ccaacaaaat gggtttgcgt accctgcttg aatggaaagt atgcgcggat gacccggtaa
      901 aaatggcatc aagtgcattt tccagtatct acacactgga taaaaaagtt tatgacttgt
      961 acgaatccgc agaaattcac acggtaaaca aagtcaagcg ttcaaaatgg ttttatgcat
     1021 tgcccgtcat catattattg attccgctat ttgtcggttt gtcttacaaa atgttgggca
     1081 gttacggaaa aaaacaggaa gaacccgcag cacaagaatc ggcggcaaca gaacagcagg
     1141 cagtacttcc ggataaaaca gaaggagaat cggtgaataa cggaaacctt acggcagata
     1201 tgtttgttcc gacattgccc gaaaaacccg aaagcaagcc gatttataac ggtgtaaggc
     1261 aggtaaggac ctttgaatat atagcaggct gtatagaagg cggaagaacc ggatgcacct
     1321 gctattcgca tcaagggacg gcattgaaag aagtgacgga gttgatgtgc aaggactatg
     1381 taaaaaacgg cttgccgttt aacccataca aagaagaaag ccaagggcag gaagttcagc
     1441 aaagcgcgca gcaacattcg gacagggcgc aagttgccac cttgggcgga aaaccgcagc
     1501 agaacctaat gtacgacaat tgggaagaac gcgggaaacc gtttgaagga atcggcgggg
     1561 gcgtggtcgg atcggcaaac tgaagaaaac ggcaacagag aaaaaagacc cgtaaaccgt
     1621 ttgaatatag acggcttacg ggtctttgtt tcgcgcaaag caagggctaa ggcagtcagg
     1681 cagcaaatcc cgcaatgtat taaaacagac gcgtagaaat gccggctgcc tttatccatc
     1741 ctcaaaattg aatatcatcc tagccgtatc aaggctgtat aaataaggaa aataccaatg
     1801 aatataatcg ggccggacat ctcaaaggac accatagacg caacattgca taaaacaaac
     1861 ggaagtatcc attacattaa atttaagaat aatgatgatg gattaaaaca gtttagattg
     1921 tggataaagg gaaacagaat cagaaaagtc tatatcggca tggaggcaac aggcatctat
     1981 tacgaaaagg cagcagatat gctttcttcc tactataccg tttacgttat caatccctta
     2041 aaaatcaatg actacggaaa aagcaggttt aaccgtacca aaaccgacaa agcagattca
     2101 aacctgatag cagattacat aaaaaggcat caagatacat tgataccgta tcagataccc
     2161 aaaaacaaag cactgcaaaa actgattaat cttaaaaacc aattacagca acagcagaag
     2221 caaattaaaa accgtcttca tagcactgaa gaagacttca taaggaacat acatcaagac
     2281 ttgatagata ccatacagga caagatggaa caggtaaaaa tagccatatc cgaacaaatc
     2341 aaaaaacaaa cggacaataa ccattaccgc aatcttcaaa ccatcccgag cataggcaaa
     2401 gacaccgcat cagttcttta tgcgcaactg acagaaaaac attttaaaac cgcaaaccaa
     2461 tttgtatcct atgccggatt aagtcccgcc atcatacaat cagggacaag cgtaagaggt
     2521 cggggcagat tgagccgata cggaaacaga cgattaaaaa gt
//
LOCUS       DAXDZG010000127         2351 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXDZG010000127 DAXDZG010000000
VERSION     DAXDZG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.127"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            127..351
                     /locus_tag="ACFB78_002043"
     CDS             127..351
                     /locus_tag="ACFB78_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269944.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            380..1666
                     /locus_tag="ACFB78_002044"
     CDS             380..1666
                     /locus_tag="ACFB78_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC1269945.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQLGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDREWQHIKDIEYEERQISDFLNN"
     gene            1680..1991
                     /locus_tag="ACFB78_002045"
     CDS             1680..1991
                     /locus_tag="ACFB78_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269946.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            2005..2208
                     /locus_tag="ACFB78_002046"
     CDS             2005..2208
                     /locus_tag="ACFB78_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269947.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          767 a          463 c          556 g          565 t
ORIGIN      
        1 cgcgggaatg acggaggagc ggataaattc tcgcaatcta aaaccttata tattgcgctc
       61 cataaggggc gcatattttt aatccttgta aagcccctta actctagtca gtgaaggggc
      121 ttaatcatgt ttattttcta tactgtcaat cccgaacacg tatatttccc caaagcatac
      181 atcatgaaag tgttcaagga taaaggctac gaatcccaat gcatcacaac agtttctttt
      241 tatatctgca atccgacttt gaagcaaaag accgaaaacg aagcgtatga atacggcaga
      301 ttgtttgtta aagaactgat gcataaggaa tgcaaccgtg aaagcctttg aagataaagc
      361 cttggcggtg tccgcggaca tggcggacgc gttagcggac ggcggggctg cggcaaccgc
      421 cgtaggctgc ccccctaggc taataggggg ggagcaaaat aaaaccccaa atccgaaggg
      481 tgcggaaaaa acagaaaacc aagaatttca attcgagtat ttcagtcatt ttgtatcgga
      541 cggcaaaggc agatttatag aaataccgtt aaggcgtggc agggatgacg gagcgttcat
      601 agaccaaatc actttcacga tacatgaaaa ttccatgacg aaagtaaccg gaaagggatt
      661 ggtatcagat acggaattcg ttgtccggta tagcgagctg cttgaagaaa tcttgggatt
      721 cggcattacc aaaaaattac cgtttaaagg aaagtttttc tatcaaagct gttatcaatt
      781 cgggcccgat aacgtcgaat acggaaaggt tcattacggc ggccaatgtg aaacgatgtt
      841 ggttgaattg aacggtacag gctgcatggc tgctttaccc ggttgggaaa accgattgta
      901 tgaattttta agtaaatgcg tccgacccaa aataacccgt atcgatgtcg cccatgattt
      961 tttcaacgga gagtacacac ccgatcaggc aatgttagac cacgataacg gacattacga
     1021 cgtacacaat atgcggccaa aaagcgaatg tcgcggcacg gcatggcgca atgaagacgg
     1081 cagtggaaaa accttttaca tcggcaagcg cggaaattca aaatttaccc gtgtttacga
     1141 gaaaggcaga cagcttggag atgttgatag cccttgggtc aggtttgagg gggatataga
     1201 aatacccttg gatgtcctac tttattcggg gtcgtatcta ggcggcgcat atccgatttg
     1261 caaggagatt tttaagacag aagccaagcg catggaagta aaagttaaaa acgttaatct
     1321 gatattcgac gttaagcttt ttcatgcaag gaatcaagtc ggcaagatgg ttaatttcct
     1381 ccgcgatata gggtgggacg atagcaggat agttgatgag ctggtgaaag gcgttgaagg
     1441 ttatccaaaa ggtttacagc ctgagcaata cgattgcaaa aaccaaacgc agaaattcca
     1501 gtatatccac gaagaacaga aagccattaa cgcattgaac attgaaacac tgtttgatga
     1561 tttgattgaa gagagagaat acgcatttcc tcaagacagg gaatggcaac acatcaagga
     1621 tattgagtac gaggaacgtc aaatatccga ttttttaaac aactgaagga aattgaaaaa
     1681 tgtttgaaac aagccaagta accacatata ccgccacttt gttgggcgca aaaaaattca
     1741 aaggcgaaat cgacggtaac aaaatcgatt catgcaccgt cttggtagcc agccccatgc
     1801 cgtcaaacgg caatgccgtg ggctttacct cggaaagcat gaagtttggt gacagccata
     1861 attttgaaaa attgaaaaac cttaaattcc cctgtgcggt tgatctaacc gttgccatgg
     1921 aatcaacagg aaaaggctta gtgcagaaat tgttggattt ccaagtaaag ggcgcagcac
     1981 ctaaagccta agaaaggctg aatcatgagc agataccaac agaaatttat cgtacaggaa
     2041 ttggaaaatc acgaattcat ctatcccgat tcgtttggcg atattggatt tacgtcgaac
     2101 atcaaatcag ccggtaaata tgacagctat gaagacgcat tcagctcggc attggaagag
     2161 ataggcgggg aatttgtaat tttcggattc tacgaaaaag aagattaagt ttaagaggct
     2221 cggcaggcgg tctctaaaac cttcacaaag cccgcaaaca cattttttta aaaatttcgt
     2281 aaaggaaaat atcatgaaat ttattaacac ctgccgtaaa tacggcgcaa aactggctgt
     2341 tgtaacagcc g
//
LOCUS       DAXDZG010000128         2325 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXDZG010000128 DAXDZG010000000
VERSION     DAXDZG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.128"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            172..366
                     /locus_tag="ACFB78_002047"
     CDS             172..366
                     /locus_tag="ACFB78_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1269948.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPMM"
     gene            complement(422..506)
                     /locus_tag="ACFB78_002048"
     tRNA            complement(422..506)
                     /locus_tag="ACFB78_002048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="ACFB78_002049"
     CDS             657..1331
                     /locus_tag="ACFB78_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269949.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB78_002050"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB78_002050"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC1269950.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..2267
                     /locus_tag="ACFB78_002051"
                     /pseudo
     CDS             2085..2267
                     /locus_tag="ACFB78_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          570 a          535 c          657 g          563 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccgatg
      361 atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg caccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaacgc
     2281 cgtaggatta gacatatcca agctgacatt tgacgcatcc gccat
//
LOCUS       DAXDZG010000129         2304 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXDZG010000129 DAXDZG010000000
VERSION     DAXDZG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.129"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>2304
                     /gene="pilC"
                     /locus_tag="ACFB78_002052"
     CDS             <1..>2304
                     /gene="pilC"
                     /locus_tag="ACFB78_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC1269951.1"
                     /translation="RGNAVNWIHTTRAGLSGYSYTGVTCRSSKCPQLVYKTRFSFDNT
                     GLAKNAGKLDRYTDLSRENSPIYKLKDHPWLGVSFNLGAEGTDKDGRSSNKLVSSFDE
                     NNSNQNLVYTTEGHRISLGDWQREHTAMAYYLNAKLHLLDKKQIKNIAPGKTVDLGTL
                     EPRVETTGRSWLDFWATWKIEDKGNITVRLGLPEVKAGRCTNAAHPNPKVKPLSPALT
                     APALWFGPAQNGKVQMYSASVSTYPGSSSSRIFLQELKTRTDPARPGRHSLAALNARD
                     IKSREPNFNSRQTVIRLPGGVYRIAPGNSGRVAGINGNDGRNDTFGIYKDRLVTPEAD
                     EWSNILLPWTARYYGNDDIFKTFNKPNNQKKYSQKYRIRTKEDDNDKPRDLGDIVNSP
                     ITAVGGYLATAANDGMVHIFKKNGGSDERSYNLKLSYIPGTMPRKDIESKDSTLAKEL
                     RAFAEKGYVGDRYGVDGGFVLRRITDDQDRQKHFFMFGAMGLGGRGAYALDLTKIDSN
                     NLTGVSMFDVKDGDNNGKNRVEVKLGYTVGTPQIGKTQNGKYAAFLASGYAAKDIVSS
                     DNTTALYVYDLGNGSGSLIKKIEAPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMY
                     RFDLSNSDSSKWSAKVIFEGDKPITSAPAVSRLADKRVVIFGTGSDLSEEDVVGKDQQ
                     YIYGIFDDDKGTGTVKVTVQNGTGGGLLEQTLTKENNTLFLSNNKASGGSNGKGWVVK
                     LRQGERVTVKPTVVLRTAFVTIRKYKD"
BASE COUNT          631 a          613 c          610 g          450 t
ORIGIN      
        1 agcgcggcaa tgccgtcaat tggattcata ccacccgggc cgggctgtca ggctactcct
       61 acaccggcgt cacatgcaga agcagcaaat gcccccaact tgtctataaa acccgatttt
      121 ccttcgacaa caccggtttg gcaaaaaatg cgggcaagct ggataggtac acagacctaa
      181 gccgcgaaaa ttcgcccatt tacaaattga aagatcatcc atggttgggc gtgtctttca
      241 atttgggcgc cgagggtacc gacaaagatg gcagatcatc caacaaattg gtatcttctt
      301 ttgatgaaaa caatagtaat caaaacctcg tctatacgac agaaggccac cgtatttccc
      361 ttggcgactg gcagcgcgaa cataccgcca tggcctatta tctgaacgcc aaactgcacc
      421 tgctggataa aaaacagatt aaaaatatcg cgccaggcaa aacagtggat ttgggcacct
      481 tggaaccgcg cgtcgagacg acaggacgaa gctggctaga tttttgggct acgtggaaga
      541 ttgaagataa agggaacatt acagtccgcc tcggcctgcc ggaagtcaaa gccgggcgct
      601 gcaccaacgc cgcccacccc aatcccaagg tcaaacccct ttcgccggca ctgaccgccc
      661 ccgcgctgtg gttcggtcct gcgcaaaatg gcaaggtgca gatgtattcc gcttcggttt
      721 ccacctaccc cggcagttcg agcagccgca tcttcctcca agagctgaaa actcgaaccg
      781 accccgcccg gcccggccgg cattccctcg ccgctttgaa tgcgcgggat atcaaatccc
      841 gcgagccgaa tttcaactca aggcagaccg tcatccgatt gccgggcggc gtgtaccgga
      901 tcgccccggg caatagcggc cgggtcgcgg gtattaatgg caatgacggc agaaacgaca
      961 ctttcggcat ctacaaggac aggctcgtca cacctgaggc cgacgagtgg agcaacatac
     1021 tgctgccttg gacggctcgg tattacggta atgacgatat atttaaaaca ttcaacaaac
     1081 caaacaacca aaaaaaatac agccaaaaat accgcatccg cacaaaagaa gatgacaatg
     1141 acaaaccccg cgatttgggc gacatcgtca acagcccgat aacggcggtc ggcgggtatt
     1201 tggcaaccgc cgcgaacgac gggatggtgc atatcttcaa aaaaaacggc ggcagtgatg
     1261 aacgcagcta caatctgaag ctcagctaca tccccggtac gatgccgcgc aaggatattg
     1321 aaagcaaaga ttctacactc gccaaagagc tgcgcgcctt tgccgaaaaa ggctatgtgg
     1381 gcgaccgcta cggcgtggac ggcggctttg tcttgcgccg cattacagat gaccaggaca
     1441 ggcaaaaaca tttctttatg tttggtgcga tgggccttgg cggcagaggc gcatacgcct
     1501 tggatttgac caaaatcgac agcaacaacc tgaccggcgt ttccatgttt gatgtcaaag
     1561 atggcgataa taacggcaaa aatcgcgtgg aagtgaaatt aggctacacc gtcggcacgc
     1621 cgcaaatcgg caaaacccaa aacggcaaat acgccgcctt cctcgcctcc ggttatgcgg
     1681 ctaaagatat tgtcagcagc gataatacaa ccgcgctgta tgtgtatgat ttgggaaacg
     1741 gcagtggtag tctgattaaa aaaatcgaag cacccggcgg caagggcggg ctttcgtccc
     1801 ccacgctggt ggataaagat ttggacggca cagtcgatat cgcctatgcc ggcgaccggg
     1861 gcggcaatat gtaccgcttt gatttgagca attccgattc tagtaaatgg tctgcaaagg
     1921 ttattttcga aggcgacaag ccgattacct ccgcgcccgc cgtttcccga ctggcagaca
     1981 aacgcgtggt tatcttcggc acgggcagcg atttgagtga agaggatgta gtcggtaagg
     2041 atcaacaata tatttacggt atctttgacg acgataaggg gacggggacg gttaaggtaa
     2101 cggtacaaaa cggcacggga ggcgggctgc tcgagcaaac gcttacgaag gaaaataaca
     2161 ccttgttcct gagcaataat aaggcatccg gcggatcgaa cggcaaaggg tgggtagtga
     2221 aattgaggca aggagaacgc gttaccgtca aaccgaccgt ggtattgcgt accgccttcg
     2281 tgaccatccg caagtataaa gacg
//
LOCUS       DAXDZG010000130         2289 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXDZG010000130 DAXDZG010000000
VERSION     DAXDZG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.130"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>2289
                     /gene="pilC"
                     /locus_tag="ACFB78_002053"
     CDS             <1..>2289
                     /gene="pilC"
                     /locus_tag="ACFB78_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC1269952.1"
                     /translation="RNNAVDWIRTTRIALAGYSYEGVVCRSGTGCPKLVYKTRFSFDN
                     PDLVKNAGKLDRYTDLSRENSPIYKLKDYPWLGVSFNLSSENTVKDSKLSNKLISSFS
                     ESNNNQTIVSTTEDHPISLGGSQREHTAVAYYLNAKLHLLDKKGIGDIAPGKTVRLGL
                     LKPSIDVRKGNTGLSGILSFNATWDIKDTGQIPVKLGLQVKAGRCINKPNPNKNTKAP
                     SPALTAPALWFGPRQDGKVQMYSASVSTYPDSSSSKIFLQNLSRNDDKNKPGRYSLKP
                     LSENEIKSKEPRFNGRQTVIRLDSGVQLIKLNGSKDEVVIFGNNGNNGTFGIVKEANV
                     NLEADEWKKVLLPWTVRGPDNDNKFKLINQKPDRYSQRYRIRENGNRDLGDIVNSPIV
                     AVGGYLATSANDGMVHIFKKTGTDERSYNLKLSYIPGTMERKDIEGNDSDLAKELRTF
                     AEKGYVGDRYGVDGGFVLRQVNLNGQDRVFMFGAMGLGGRGAYALDLSKINENYPAAA
                     PLFDVKNGDKNGKNGKNRVEVELGYTVGTPQIGKTRSGKYAAFLASGYAAKDIGSGDN
                     KTALYVYDLNNTLGTPIAKIEVKDGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYRF
                     DLSNSDPNKWSVRTIFEGTKPITSAPAVSRLKDKRVVIFGTGSDLTEDDVLDTKEQYI
                     YGIFDDDKAANNVNASRGVLGSGLLEQHLTQENKTLFLNKRSDGSGSKGWAVKLTGGR
                     RVTVKPTVVLRTAFVTIRKYKD"
BASE COUNT          657 a          571 c          582 g          479 t
ORIGIN      
        1 agcgcaacaa tgccgttgat tggattcgta ccacccgcat cgcgctggca ggctacagct
       61 acgaaggtgt cgtatgcaga agcggcacag gctgtcccaa acttgtctat aaaacccgat
      121 tttccttcga taatcccgac ttggtaaaaa atgcgggcaa gctggatagg tacacagacc
      181 taagccgcga aaattcgccc atttacaaat tgaaggatta tccatggttg ggcgtatctt
      241 tcaatttgag cagcgagaat accgtcaaag atagcaaatt atccaacaaa ttgatatctt
      301 cttttagtga aagcaataat aatcaaacca tcgtctctac gacagaagac caccctattt
      361 cccttggcgg ctcgcagcgc gaacataccg ccgtggccta ttatctgaac gccaaactgc
      421 acctgctgga taaaaaaggg attggagata tcgcgccagg caaaacagtg cggttgggtc
      481 tcttgaagcc gagcatcgat gtgcggaaag gaaatacggg gcttagcggc attctatctt
      541 ttaatgctac gtgggacatt aaagataccg ggcagattcc ggtcaagctc ggcctgcaag
      601 tcaaagcagg ccgctgcatc aataaaccga accccaataa gaataccaaa gccccttcgc
      661 cggcactgac cgcccccgcg ctgtggttcg gacctaggca agatggtaag gtgcagatgt
      721 attccgcttc ggtttccacc taccccgaca gttcgagcag caaaattttc ctgcaaaacc
      781 tttcccgcaa tgatgacaaa aacaaaccgg gccgctattc cctcaaaccc ttgagtgaga
      841 atgagattaa aagtaaagag ccgcgtttca acggaaggca gaccgtcatc cgattggata
      901 gcggcgtaca gctgatcaaa ctgaatggaa gcaaggatga ggtcgtcatt tttggaaaca
      961 acggcaacaa cggcactttc ggcattgtta aggaagcgaa cgtcaatctt gaagccgacg
     1021 agtggaaaaa agtgctgctg ccttggacgg ttcggggtcc cgataatgac aataaattta
     1081 aattaattaa ccaaaaacca gacagataca gccaaagata ccgcatccgc gaaaacggca
     1141 atcgcgattt gggcgacatc gtcaacagcc cgattgtcgc ggtcggcggg tatctggcaa
     1201 cttctgccaa cgacgggatg gtgcatatct tcaaaaaaac cggcacggat gaacgcagct
     1261 acaatctgaa gctcagctac atccccggta cgatggagcg taaggatatt gaaggcaatg
     1321 actccgacct cgccaaagag ctgcgcacct ttgccgaaaa aggctatgtg ggcgaccgct
     1381 acggcgtgga cggcggcttt gtcttgcgtc aagttaactt aaacggacaa gaccgcgtgt
     1441 ttatgttcgg cgcaatgggc cttggcggca gaggcgcata cgccttggat ttaagcaaaa
     1501 tcaacgaaaa ttatccagcc gccgctcccc tgtttgatgt caaaaatggc gataaaaacg
     1561 gcaaaaacgg caaaaatcgc gtggaagtgg aattaggcta caccgtcggt acgccgcaaa
     1621 tcggcaaaac ccgcagcggc aaatacgccg ccttcctcgc ttccggttat gcggctaaag
     1681 atattggcag cggcgataat aaaaccgcgc tgtatgtata tgatttgaac aacaccttag
     1741 gtacgccgat tgcaaaaatc gaagtgaagg acggcaaagg cgggctttcg tcccccacgc
     1801 tggtggataa agatttggac ggcacggtcg atatcgccta tgccggcgac cggggcggca
     1861 atatgtaccg ctttgatttg agcaattccg atcctaataa atggtctgta aggactattt
     1921 tcgaaggcac aaaaccgatt acctccgcgc ccgccgtttc ccgactgaaa gacaaacgcg
     1981 tcgtcatctt cggtacgggc agcgatttga ccgaagatga tgtactcgat acgaaagaac
     2041 aatatattta cggtatcttt gacgacgata aggcggcgaa taatgtaaat gcaagccgcg
     2101 gcgttttggg gagcgggctg ctcgagcaac accttactca ggaaaataaa acattattcc
     2161 tgaacaagag atccgacggt tcgggcagca agggctgggc ggtgaaattg acaggcggac
     2221 ggcgcgttac cgtcaaaccg accgtggtat tgcgtaccgc cttcgtaacc atccgcaaat
     2281 ataaagacg
//
LOCUS       DAXDZG010000131         2053 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXDZG010000131 DAXDZG010000000
VERSION     DAXDZG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.131"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(44..1012)
                     /gene="ispH"
                     /locus_tag="ACFB78_002054"
                     /gene_synonym="lytB"
     CDS             complement(44..1012)
                     /gene="ispH"
                     /locus_tag="ACFB78_002054"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC1269953.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1042..1536)
                     /gene="lspA"
                     /locus_tag="ACFB78_002055"
     CDS             complement(1042..1536)
                     /gene="lspA"
                     /locus_tag="ACFB78_002055"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC1269954.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          461 a          592 c          525 g          475 t
ORIGIN      
        1 atttgtattg cccaaacatc cgatgcctgc gttaacgcct gcgtcaacgt ttgcacaaat
       61 cgggtttggt ttcgccctcg cggcgcagct ccttgggcag gacgaacacg atgctttctt
      121 ccgcgccccc cccttcgcgc acggtttcat gcccccatcc gcgtatggct gccaatactt
      181 cccgcaccaa cacttcgggc gcggacgcgc ctgccgttac gccgactttg cttttgcctt
      241 caaaccacgt gcgttgcagg taggacgcgt tgtccaccat atacgcatcg attccgcgcg
      301 atgccgccac ttcgcgcagg cggttgctgt tggacgaatt gggcgaaccg accacaatca
      361 cgatgtcgca ctgttccgcc agctctttga cggcggtttg ccggttggtc gtcgcatagc
      421 agatgtcttc cttgtgcgga ttgcggatat tggggaaacg cgcgttcagc gcggcgatga
      481 tgtctttggt ttcatcgacc gagagcgtgg tttggctgac ataggcgagt ttgtcggggt
      541 ttctgacttc gagttttgcc acatctccga ccgtttcgac caaaagcatt ttgcccggtg
      601 caagctgccc catcgtgcct tcgacctcgg cgtgcccctt atgcccgatc atgatgattt
      661 cacagttttg ggcatccagt cgggcgactt ccttatgcac tttcgtcacc agcgggcaag
      721 tcgcatcaaa tacccggaaa ccgcgctccg ccgcttcctg ctgcaccgcc ttcgatacgc
      781 cgtgtgccga ataaaccagt gtcgcgcccg gcggcacttc cgccaagtct tcgataaaca
      841 ccgcgccttt ttcgcgcagg ttgtccacga cgaatttgtt gtggacgact tcgtggcgca
      901 cataaaccgg cgcgccgaat tcttccaaag cacgttcgac aatactgatt gcccgatcca
      961 caccggcgca gaagccgcgc ggattggcaa ggatgatggt ttttccgttc ataagttttg
     1021 cattccgtgt tcagacggca ttcacgtttt tttgctatct ttgcgatgga cgatattgtc
     1081 aagcaccgcc aacaccgcac cgacgcagat aaagctgtcg gcaatattaa aggcgggata
     1141 aaaccaattt tgccaataaa acaataagaa atcgacgaca tgaccgtgta tcaggcgatc
     1201 gatgacattg cccgacgcac cgccgataat cattgccgcc ccgattttgc cgagggctgc
     1261 aaactcgtcg cgcaagatgg cgcgtaccaa atacgcgctc accgccaccg ccagcaccaa
     1321 aaaaaagtat ttctgccagc cgccctgatc ggcaaggaag ctgaatgccg cgcccgggtt
     1381 gtacaccagc gtcagatcga aaaacgaagg gatgacgttg acgcgttccc gatactgaaa
     1441 cgacgacagc accgcccact tcgaccactg gtccagcacg atggcggcaa gtgccaatac
     1501 ccaatagcgc gttttacttg aaacagatga agacatattt ttcaacagcc ggataaaaga
     1561 gtgccattct acccgaaaac cccctttcct gtacccgaaa cggcaaatgc cgtaatcttg
     1621 aaacccgtca ttcccgcgca ggcgggaatc cggacctgtc cgcacggaaa cttatcggat
     1681 aaaacggttg cccaaacccc gcgtcctaga ttcccacttt cgcgggaatg acggttcggt
     1741 tgcgtagggc cgggtggtcg aaaaggcgga ttcgatggat tcgatgaaaa cggtagacat
     1801 gttggattga tggaaatggc gggccgaagc acaccctacg acccgccgga tttccgcctg
     1861 cgcgggaatg acggggtttc aggttgctgt ttcaggtttc tgtttttaag tttctgtttc
     1921 ggtttgctat tttatagaaa tgacgaaatt ttagattgca agaacttatc ccctccgccg
     1981 tcattcccac ggaagtggga atctagaaat aaaaagcagc aggaatttat cggaaataac
     2041 tgaaaccgaa cgg
//
LOCUS       DAXDZG010000132         2027 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXDZG010000132 DAXDZG010000000
VERSION     DAXDZG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.132"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..279
                     /locus_tag="ACFB78_002056"
     CDS             <1..279
                     /locus_tag="ACFB78_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFC1269955.1"
                     /translation="LCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQTLATIAAV
                     CKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            395..754
                     /locus_tag="ACFB78_002057"
     CDS             395..754
                     /locus_tag="ACFB78_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269956.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
     gene            897..1520
                     /locus_tag="ACFB78_002058"
     CDS             897..1520
                     /locus_tag="ACFB78_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269957.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFI
                     LIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKY
                     YNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
     gene            1580..>2027
                     /gene="cas1"
                     /locus_tag="ACFB78_002059"
     CDS             1580..>2027
                     /gene="cas1"
                     /locus_tag="ACFB78_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC1269958.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQERKKVAQLPVHSIGHI
                     FCFGNVLVSPFLLGFCGENNVNLAFFTENGCYLGRLQGRQNGNVLLRRAQYRVSEQSP
                     MPIACNIIAAKIRAIRKRARACALSGQCMFWRISWASAAYWIWWKWI"
BASE COUNT          637 a          349 c          468 g          573 t
ORIGIN      
        1 ttgtgtttgg aagaaatgac ggggcaaacc gtttcagagg gcgcgctgtg gtatatgcaa
       61 acccgccacc gcgtccccgt tgtgttttca gacggtctgc gcgcccaaac cctcgccacc
      121 atcgccgctg tgtgcaaact cctaaacagc ggccaaaccc cgccgcccga ctacggcaaa
      181 cgctgcaaag cctgctcgct ggcggagatt tgccagccgg agttgctggg aaaacgggat
      241 aggagtgtgg gatatgtgaa ggggttgttt ggggagtaat attttatttg aaaaaaatta
      301 ttacgttaat taacttgaat cttccaaatt aatcgcaatt tctgaaaacg agcaatcatg
      361 ctcaattgta attacacatt ttaaggagtc gtaaatgaca agaaatatag aacatcaatt
      421 tagcaatcta tctgatgttg gtgaaaaact agagttagaa aatcctactg tagagaatgt
      481 cgtagatatt ttagtggata ttggacatga tgaccgagtt tatacttttc atgatgactt
      541 tctcgggtta aaaagcggtt tgccacaaga cttattgtcg aaacatatcg acgagcttga
      601 agaaggtgat tttgcagata gatattcaga tgaaattgat aaaatactgg ataatgcaaa
      661 tataatattc tatcaccttg aacgagagct tagtgaagat gatttagagg aaatacgaga
      721 agaaagagaa agactgggtt tagaagacga ttagtatact tgttgtcata gaatagattc
      781 aatacataca ggttgggtta tgctgtttta cccaaccagc taacctcagg gcatatttaa
      841 tattatttaa ttctgaataa tattctattg aagtttcaag atatctaagg aaaaacatga
      901 cacaaaaaaa tgattttaaa aatgctaatt tttatgggaa tactcaaata gttaatggtg
      961 atgttcataa tgtacatact caaaatacac caaattcaat aaatgaaaac gaaatagcca
     1021 catacacccc ggaaccaatt tggagaagcc ccattacgat ggcttggctg acatgggcta
     1081 gttttctgat ttctttagca gggctattac ctatttataa ttgtatagtt aaacccatat
     1141 tatatttagt aaatggtaaa actatgatac aaagtgaaat aaataatact tatatattta
     1201 ttttaatagc tattgctgta ttatttctta tcactattac attacgcaga attactaagt
     1261 atcaaactag atacccatta gtttttaatt atgctattag cggtatgggg catcgtttaa
     1321 gtctagagaa aattagtatt tctcaatgtc cacagtgcgg tggagaaatg aaatattata
     1381 ataagccaat cgagtgggag aatatacgtt attctgatgg tagcaagaaa agaagggtaa
     1441 caagaactac accagcatta gagtgtaaaa gaaatcgtga gcattggtat aaagttgatc
     1501 cggcagaaga taaaatctaa tttttagcta gcccctcgtt taaccccaaa agcacggtca
     1561 aaacctaagg agttttccca tgcgcaaact gcaaaacaca ctctacatca ccacccaagg
     1621 cagctatctg cataaggagc gggagacact ggtggtggag caggagcgta agaaggtggc
     1681 gcagttaccg gtgcattcca tcgggcatat tttctgtttt gggaatgtgc ttgtgtcgcc
     1741 gtttctgttg gggttttgcg gcgaaaataa tgtgaatttg gcgtttttta ccgaaaacgg
     1801 gtgttatttg ggacggcttc aggggcggca gaacggcaat gtgctgttgc gtcgggcgca
     1861 gtatcgggtg tcggagcaaa gtcccatgcc gattgcgtgc aatatcattg cggcaaagat
     1921 tcgggcgatc cggaaacgtg caagggcgtg cgctttgagt ggacagtgca tgttttggcg
     1981 gataagctgg gcatcagcgg catattggat ttggtggaag tggatac
//
LOCUS       DAXDZG010000133         1915 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXDZG010000133 DAXDZG010000000
VERSION     DAXDZG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.133"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..1248
                     /locus_tag="ACFB78_002060"
     CDS             <1..1248
                     /locus_tag="ACFB78_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC1269959.1"
                     /translation="PLSNRGGTETETAGRVQEVFECYETYITDGKGNLLGVPLRRGVS
                     DSAFIDQITFSIHEDTFFHVYGLSFDLFDDDDFIRAASAKMEEIFGFGIIEKAKHSGG
                     RFYEGCWLMGTENAQYGRVHYGGQRETMLVELTAVGCNAANIGWESRLFDFLTNAIRP
                     KITRVDIAKDFFNGEYSPNQAREDRNKGLFTCHHVKPKGECLGSDWEEDDEAKMTKGK
                     TYGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGA
                     YPICERFAQKATRIHAVKEDKVVSADRCLEWVKKQFGRAANGLKFIFPELDKAKLFEL
                     IEPSHHKLPKSLALEAYDCAFLKSQVIHEQPAFKPYNDPYYMYEYYENLEKQLEQQKH
                     VNNEESYNNFIYDKFARLPISWA"
     gene            1293..1583
                     /locus_tag="ACFB78_002061"
     CDS             1293..1583
                     /locus_tag="ACFB78_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269960.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            1588..1785
                     /locus_tag="ACFB78_002062"
     CDS             1588..1785
                     /locus_tag="ACFB78_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269961.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
BASE COUNT          582 a          429 c          442 g          462 t
ORIGIN      
        1 cccttatcta acaggggggg tacagaaacc gaaacggcgg gcagggttca ggaagtcttc
       61 gaatgttacg aaacgtacat aacggacggt aaaggaaacc tgttaggcgt tcctcttagg
      121 cgcggtgtat cagattcggc tttcattgac caaatcacat tttcaattca tgaagacacg
      181 tttttccatg tttatggact ttcgtttgat ttgttcgatg atgacgattt catacgcgcg
      241 gcttccgcca agatggagga aattttcgga ttcggaatca ttgaaaaagc caagcattcg
      301 ggcgggcgtt tctatgaagg ctgctggctg atgggaacgg aaaatgccca atacgggcgc
      361 gtccattacg gcggccaacg tgaaacgatg ttggttgaat tgacggcggt aggctgcaat
      421 gctgcaaata ttggatggga atcaaggctt tttgattttc tgacaaatgc gattcgtcca
      481 aaaataacgc gcgttgacat tgcaaaagac tttttcaacg gagagtacag cccgaatcag
      541 gcaagagaag accgcaataa aggtttgttt acgtgccatc acgtcaaacc gaagggcgaa
      601 tgtttggggt cggattggga agaagacgat gaagccaaaa tgaccaaagg caagacctat
      661 ggtatcggct cccgtgaatc gtccaaatat gtccgcgtct atgaaaaagg caagcagttg
      721 ggcgataaaa caagcacatg gacgcgcttt gaaattgaat tcaaagcaaa agacatcgtt
      781 atccctttcg aagttttgca gaatccgggc gaatatttcg gcggcgcata tcccatttgc
      841 gaacgattcg cccaaaaggc aacgcgcata cacgcggtta aggaagataa ggttgtttca
      901 gccgaccgct gccttgaatg ggtaaaaaaa cagttcggac gtgcggcaaa cggtctgaaa
      961 ttcatttttc ccgaattgga caaagccaaa ctgtttgaac tgattgagcc gagtcatcac
     1021 aagctgccca agtctttggc tctcgaagcc tacgactgcg cctttttgaa atctcaagtc
     1081 attcatgaac agcccgcatt caaaccgtac aatgaccctt attatatgta cgaatattac
     1141 gagaatcttg aaaaacagct tgaacagcaa aaacacgtca acaatgaaga aagctataac
     1201 aacttcattt acgacaaatt cgcaagacta ccgatttcat gggcttaaag tgtctgcccg
     1261 aaagacgttt aatcacacaa ggaaaccaaa aaatgaacat ccaacttcaa ggccacatcg
     1321 tcggcgttaa aaaattcaac ggacaaatcg aaggcaagag cttcgactat tgccgcctga
     1381 ttgtcgccac acccttagac agctcccaag gcaacgcatt gggcagctct actactgaat
     1441 acgatttcgg cggctctgcc aatttcgagc agttccgaaa cgcccaattt ccgatcgaag
     1501 caaacctgaa cgtggaaatc gtcactacgg gcaaaactca aaaactgaaa gtcaccggtt
     1561 ttcaattcgt taagaaaggc tgattgaatg cagaaagtct atgttgtcca gtccgtatca
     1621 acaggggact ttctgtatct ctctcctgaa acgggcgaca tcggacatac caaattaatt
     1681 accaatgccg attatttcta cgacttcgaa gaagcggtta acgcaggttt ggaagaaatc
     1741 ggcaaccaat gcgaatttgt cgtattcgga tttttgaaag actgattttc ggatgttcgg
     1801 cggtcgtctg aaaaactctc catccattac cgccaaacac tttttgaagg aaaatatcat
     1861 gaaatttatt aacacctgcc gtaaatacgg cgcaaaactg gctgttgtaa cagcc
//
LOCUS       DAXDZG010000134         1885 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXDZG010000134 DAXDZG010000000
VERSION     DAXDZG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.134"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFB78_002063"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFB78_002063"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC1269962.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..>1885)
                     /locus_tag="ACFB78_002064"
     CDS             complement(1558..>1885)
                     /locus_tag="ACFB78_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC1269963.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCADYDGEGVVRDYRFTHKVEKDERSVIRDTVGVIRKEAGKS
                     LSQPEK"
BASE COUNT          409 a          531 c          527 g          418 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgcggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttccacttt atgtgtaaag cggtagtccc ggacgactcc
     1681 ctccccgtcg taatccgcgc accattccca atgccgccgc tccgatttca tatagatgaa
     1741 attggtcggc aaaaaattat aaatcggcag gctgacctcg tgataggcat aacaaccgaa
     1801 agggttgcgc ttcccgaaac gtgcctctac ctccgcccgg gtcgttttgc ctttaacaac
     1861 cgtttgtgcg attccctctt ccgtc
//
LOCUS       DAXDZG010000135         1862 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXDZG010000135 DAXDZG010000000
VERSION     DAXDZG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.135"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            536..754
                     /locus_tag="ACFB78_002065"
     CDS             536..754
                     /locus_tag="ACFB78_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269964.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(771..1130)
                     /locus_tag="ACFB78_002066"
     CDS             complement(771..1130)
                     /locus_tag="ACFB78_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269965.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRYNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1131..1670)
                     /locus_tag="ACFB78_002067"
     CDS             complement(1131..1670)
                     /locus_tag="ACFB78_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC1269966.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
BASE COUNT          454 a          407 c          479 g          522 t
ORIGIN      
        1 cgtcctttgc cctcgcgcca aaccgattta ttctgctgat taattcgttc atcccgtttc
       61 cttcaagttg ttgtttgttt cgatgggtgt agtttagtaa acacttaaca tctagtcaag
      121 taaaatgttt agtgattatt taataaatac tgaacatttt gattattaaa agaatttatt
      181 tttgagattt cgcaggcgca aaaaaaaccg cctattaagg cggttgttta ggaaagaatg
      241 ccctctgcgc gtgtagggta tttcgatact tgcgtttatt atatcatatg atataataaa
      301 ctcatagatt aggaaatgaa gaaaccccgt atcagccgat acggggcaac ctgaaagaga
      361 aaggaggcaa tgtgattaag aaaatcctaa tctgtgtgat tttgtatttc cttgcaagct
      421 ccccagcgtg gtagggaaat acaaaacaag tgattaacga ttaaccattt gcagggcagt
      481 taaggaggct gcccttcctc ttatctttcc catactctac tataaggatt taaaaatggc
      541 aaattccaac agcgggcatt ctaaaaaatt gcgcgcggca acggcggcgg cggcgacaaa
      601 ggcaaaactg gcaagcggcg aataccggca gttttcagtg caggggcgtg ccgaagacgt
      661 ggagcttatc cttgccgccg tggaaaaagc cgggggcagc cgcgtacagg ctttggcaaa
      721 gatttgcagg cggtatctcg aggggctgtc ttaagggggg taagacaagg ttatttggga
      781 atatcgggca ggataaaagg cgggtgtctg tcaaaaccga tgattttgat aaagccgtcg
      841 caaagggtat agtgaaggat gtaggcggat gtcaaatcgc catacctgcc gccgttgtat
      901 ttgggaatgc cgatgtgata gtgccaaagg ttgtaccgtt gggcaaatcg gactttttcc
      961 agccattgag gatcgtctgc cggtacgttg tcgctggatt tgttcctgcc gggtaaccct
     1021 gataagccgt tctgttggac gtgtgcgata aattccgcaa tttttctgcg gtcttctttg
     1081 gggtagtttt tcaacgctct tttgaagtct tcgccaagca gcacctgcat ttactgctcc
     1141 aaccattttt ccaagtcgtc ggcgccgttg aaatgcggga cgctgacaaa gctgtcttca
     1201 acggctgttt tgagtttttc aagattgaaa ttgtaatccg tctgcggcag cgcatggcgg
     1261 aaatattccc ccatcagcgc aatccctatg acttcgccct gccgggtctg tatccacggc
     1321 gcttcctgat gggttttatt gcgtaatgcc caagcggtgt agcagccctg ctctttacgg
     1381 acacggttga ggacaaccaa ctcttcgtcc gcatatttgt ccggttcgat atgggccgca
     1441 ggcaatgggc tgccgccgta ttttttgtag gtgcggtaaa tgcaggggac taccggaccg
     1501 tgctgccagt gttcgatatt ttcggcaaac agggggcggt taagtatggc aagggcgtag
     1561 ccttgtgcgt aatacaggag tttttgaagt ttgagattgg agatttgctc cccgtcctct
     1621 tcttcaaaag gggaaaggaa gaaatctgcc acgtcgtacg cgttcagcat tttctgtttc
     1681 cgttgttatt tttctgtata ttaaggccgc cggcccatta tttcaagaaa taatttgtgc
     1741 aaattatccg gtatcggcag gctgtccgcg ccgtcggcat atcgggcgga tatatttgaa
     1801 aaatacttgc cctgccgttt cagacggcat caatccaaca cgctccgcca aaagaccctg
     1861 cc
//
LOCUS       DAXDZG010000136         1766 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXDZG010000136 DAXDZG010000000
VERSION     DAXDZG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.136"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..320
                     /locus_tag="ACFB78_002068"
                     /pseudo
     CDS             1..320
                     /locus_tag="ACFB78_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            409..978
                     /gene="rsmD"
                     /locus_tag="ACFB78_002069"
     CDS             409..978
                     /gene="rsmD"
                     /locus_tag="ACFB78_002069"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC1269967.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIACSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1101..1352
                     /locus_tag="ACFB78_002070"
     CDS             1101..1352
                     /locus_tag="ACFB78_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC1269968.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1449..1532
                     /locus_tag="ACFB78_002071"
     tRNA            1449..1532
                     /locus_tag="ACFB78_002071"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1483..1485,aa:Tyr,seq:gta)
     gene            1563..1636
                     /locus_tag="ACFB78_002072"
     tRNA            1563..1636
                     /locus_tag="ACFB78_002072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1595..1597,aa:Gly,seq:tcc)
     gene            1646..1720
                     /locus_tag="ACFB78_002073"
     tRNA            1646..1720
                     /locus_tag="ACFB78_002073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1678..1680,aa:Thr,seq:ggt)
BASE COUNT          488 a          405 c          460 g          413 t
ORIGIN      
        1 gcacctgccg tttacaggcg cggggcaggt gcggggaaat acccgtaccg ccattgccga
       61 tacaccgtaa ccctaaaacc cgccattccc gcgcaggcgg gaatccggat ttgtcgggat
      121 tcgctcaaat cgggcggcgg cgggatggtg ttgcagatta acgatgtgga taccaacagc
      181 ggggaatttg caatctatac cgctcaggat gcatcggtaa agctggattt gttaaaaatg
      241 gaattgaaac actgcaaaga aatgttggaa caaaagacaa agaaatcgag ttgctccgca
      301 agctgaccga aaccgtttaa acagatatgc cgtctgaaaa aagttttcag gcggcatatt
      361 cttcgacagg tcttgtataa taccgtttga actttcaggc ttttgattat ggcggcaggc
      421 aaacatacca aacacagcaa ccgggtacgc attatcggcg ggcaatgccg gggcaggaaa
      481 ttgagttttg catccgccga cggactgcgc ccaacccccg acagcgtgcg tgaaaagctg
      541 tttaactggc tgggacagga tttgacggga aaaacggttt tggatctctt cggaggcagc
      601 ggcgcactcg gtatagaagc cgcttcgcgc aacgccaaac gcgtgctgat ttcggataac
      661 aaccgccaaa ccgtgcagac cttgcagaaa aacagccgcg aactgggttt ggggcaggtg
      721 caaatcgcct gttcggacgg catcgcatat ttggcaaacc taaaagagaa attcgatgtc
      781 gtcttccttg atccgccgtt tgcgtggcaa agttgggaaa gtcttttcaa tgtattgggc
      841 acacgcctga atgacggcgc atatgtttat atcgaggcag gcaggcagcc ggacaaacct
      901 gattggctga cgggatatag ggaggggaag tcggggcagg gtacatttga attaagggtt
      961 ttccaagtgg ctgaataata tgcgctttga taatcatttc cgggttgtaa acattcgttt
     1021 gcaaccgtcc ggttcaaaaa aaccttgtgc tataatccgc gcccgcccgg ttttgataat
     1081 ttagtggaaa aggaaaagaa atgtcgcttt ttattaccga cgagtgcatc aactgcgacg
     1141 tatgcgaacc cgaatgcccc aatgatgcca tttcccaagg cgaggaaatt tacgaaatca
     1201 accccaacct ctgcacgcag tgcgtcggac actacgatga gccgcagtgc cagcaggttt
     1261 gcccggtgga ctgcatcctg attgacgaag aacatcccga aacccatgac gagttgatgg
     1321 cgaaatacga aaagattatc cagtttaaat aaattctttt taaaacatca aattatgtct
     1381 gttttgaaat aaaatcaaaa aaaaacttga cggaaaagca agccgctaat aaactagtgt
     1441 tctcttttgg agggattccc gagcggtcaa agggggcaga ctgtaaatct gttgcgaaag
     1501 cttcgaaggt tcgaatcctt ctccctccac caaaattctt acttggggca gtagcgagta
     1561 atgcgggtgt agctcaatgg tagagcagaa gccttccaag cttacggtga gggttcgatt
     1621 cccttcaccc gctccaaaca attaagccca tgtagctcag gggtagagca ctcccttggt
     1681 aagggagagg tcggcagttc aaatctgccc atgggcacca tctctcgatt attcatttct
     1741 ttaaggctta gatatatagg atattg
//
LOCUS       DAXDZG010000137         1707 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXDZG010000137 DAXDZG010000000
VERSION     DAXDZG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.137"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(207..1592)
                     /locus_tag="ACFB78_002074"
                     /pseudo
     CDS             complement(207..1592)
                     /locus_tag="ACFB78_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
BASE COUNT          484 a          519 c          314 g          390 t
ORIGIN      
        1 caccctgctt gtccgatgcc tcgggacgga cgggcaaacc gacacggcac gcaaaccgcg
       61 tcggcaaaaa tgccgtctga aaaaattcag acggcatctt cagacacatt acctgcaaac
      121 ggcaacacat tccaatccag accgatcagg caatcaatac gaacggctgt ttacatactt
      181 accagctttc accaaccggt atcgatttaa ccgatttcct taatattttt cctgtccgtt
      241 ttaaacttcg ccttaaacac atccggtaaa tctttatcga aataccaaag cccgtcatcc
      301 atttccaatg cgccgcccat tccatgcaga acgacttttc ccccgttaaa ggatcggttt
      361 cggtaatttc cacctcatgc atttttcccc aatccaaaac aggcaacttg tgtgcaaacg
      421 ccaaaacttg ttcgtcaaaa cggccgcacg ccttatcgca tcggcctgaa acatatacgg
      481 gacaaggctc atcctctgca taaccgtccg gaaggatacc ggctgaggca ggacacaatc
      541 cgtatgtttc cgcccacgac gacaaagccc gtctcgccgc ctttttattg gcaaataatc
      601 cggtaggcgg attatccgtc acaccgtttt tcaaagccgc tgttttcgca ttcaacatgc
      661 cgtctgaacc tttttcaaac ctgacggtcg taaatgtttt aagcagattt ttggcagata
      721 cataacaacc cgaatgataa cgtccgacca attccgcttt aatcttatat gcatgcaggc
      781 tgcccaatgc gggaaaaaaa cggacttcct ccgtattgca ccaatcaaac gggggctttt
      841 ccggaatcca acaaagccgc aacctcgcca tatgcccgtt caaacgtacc gatataattt
      901 acttttcccc cgccgtcgaa acaggcaagc acacctgtac cgtcgggcaa accgtacagc
      961 tgttccctca accgttcggg aagcgcggca ggaagaagtt tcggattcgt caaacggaaa
     1021 cactgcctgc tccactcctc aagcccgtgt gccgacaggc tgtattccaa ataatcacac
     1081 aacgccgata catccgccat cgcacgatgc ctgtcttcca caacaatccc caacctttcg
     1141 atgataccgt ccaggctgtg cttgtaaaac tgcggataca gccgacgcga aagctgcaca
     1201 ctgcacaaag caggcgatga aaatccgata cccgcacgat gaaactcatg ctttaaaaac
     1261 gtatagtcga aacggctgtt gtgcgccacc agcacgcacc ccttcaatac cgaaaacaac
     1321 ccgccggcaa tttcagcaaa caccggcgcg tcggcaacca tgccgtctga aatccccgtc
     1381 agttccgcca caaacttcgg aatcggtttt tgaggattga ccagccattc atacctctcc
     1441 gcccttccct gcccaaactt gaccaaagcc acttcggtta ccctgtcttc atacagattg
     1501 ccgcccgtcg attccaaatc aaccacggca acaggcattc caaaccgtaa aaacgccttt
     1561 tccagcaagg gccagcggga aacaacaatc attttattct ctttaaattc aaacaacaaa
     1621 ccgatatttt acacttttaa ggcatttcat ccaacaaaat aattgacaga atccgatgat
     1681 taccataaaa ttcaaaaatt tattgca
//
LOCUS       DAXDZG010000138         1658 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXDZG010000138 DAXDZG010000000
VERSION     DAXDZG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.138"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(30..374)
                     /locus_tag="ACFB78_002075"
     CDS             complement(30..374)
                     /locus_tag="ACFB78_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269969.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKEHFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(773..1174)
                     /locus_tag="ACFB78_002076"
     CDS             complement(773..1174)
                     /locus_tag="ACFB78_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269970.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1178..>1658)
                     /locus_tag="ACFB78_002077"
     CDS             complement(1178..>1658)
                     /locus_tag="ACFB78_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269971.1"
                     /translation="GYKKPTARHMQTKALGTVDEIGNTVQQVGKQTSGQKTSGGNPAI
                     DSDPYSPSSVSARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDF
                     NSLNVRNVQTRPNGMITGNLPDGRAVNVRNDSSGGEPTLEITISNNRKIKIRYGNTR"
BASE COUNT          515 a          316 c          248 g          579 t
ORIGIN      
        1 ccaattttta ttggtttatt ggtatatcgt taatcattta tttcaaaaaa gttatttatt
       61 atcatattat cagggagcgg gtcgttaaaa aaaactccct caaattccat taaattagta
      121 tttatccagc acataaacga accaaagtgt tctttccctg agtgtaaata tttaaaatta
      181 aattcaaaat aagttttttt gcttagaaaa tttaatggtt tttctatagt gaaaaaaaag
      241 cccttactcg ttttagttcg agatactatt ttaaataagt ttttattctc aataaaataa
      301 gaatattcat ttactaagtt gttatgatga aaaagtaatt ttattaaaat gttttcataa
      361 ttttctaggt tcatttctag ctcaatttaa aatgatttca ttgttgccgt accaatgcga
      421 aatttaccat cgacaaaatt tcccttcact gatacaggta ccccatcgca gagatgtagg
      481 tcggatattt gtatccgaca aaaatatttg acgcgtttat cgtttccgaa atgccgctgt
      541 tagaaaatgt cggattcgag aatccaacct acggctatat ccgtaaagtc tgaccattgt
      601 tcaatgcgga aggcgcgaat attgcggacg gaatgtagaa accaatatac ttaattgcca
      661 tgccattctc atcaaaatac ctgtgctgtt taaagtgcgt ggttcattac acgagtaatt
      721 ttattttcag gctgcctgaa agaaagatag gcaacttatt actcatctta cgctagctct
      781 caataattga atacgttgca ctagaaccaa ttatttctat gaatgtttgc tcaaacataa
      841 aaatatagta tttaaagaat tctctattca aaggaaaata actttctttc gatatccatg
      901 ccgcataacg ctcatcaaga agttcgaaaa aagattgacc accattttca tgaagcaaag
      961 ctctatctgc atcttccgaa attaaatcca taaaagtatc tataaaatac gattcatcaa
     1021 caaccctaaa atgaataaca tcctcaatag aaatttttat ggttttaccc gtatttctat
     1081 aaccaactgc tataacttct actttatcaa agtttattgc tgcattatca aaatagaaat
     1141 agccagttgg gaaatctaag ctttttaatt tcataattta tcgtgtattt ccatatctga
     1201 tttttatttt tcggttatta ctaattgtta tttcaagtgt tggttctcca ccactgctat
     1261 cattacgaac attaaccgca cgcccatcag gtaaattgcc cgttatcatt ccattaggac
     1321 gtgtttgtac atttcgaaca ttcagactat taaaatcatt tagtgcgtca ttgtaatccc
     1381 ccactttatc atactgaaca gcgggacctt ttgttttact cctttgagta gtattgctca
     1441 aaatgtcttt gatttgtaaa tcactgcgca ccttaccggc ttctatacga gctgacacac
     1501 tactcgggct ataggggtcg ctatcaattg caggattacc accgctggtt ttttgtccgc
     1561 tagtctgttt cccaacctgc tgtactgtat tgccaatttc atctaccgta cctaacgcct
     1621 tagtctgcat atggcgggca gtaggttttt tataaccg
//
LOCUS       DAXDZG010000139         1658 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXDZG010000139 DAXDZG010000000
VERSION     DAXDZG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.139"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..684
                     /gene="mafA"
                     /locus_tag="ACFB78_002078"
     CDS             <1..684
                     /gene="mafA"
                     /locus_tag="ACFB78_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC1269972.1"
                     /translation="GYHNNPDSATRYSYPAYDTTATTKSDALSGVTTSTSVLNAPAAA
                     LTKNNGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEY
                     ADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYES
                     QYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGN
                     EVIRRRKGG"
     gene            688..>1658
                     /gene="mafB"
                     /locus_tag="ACFB78_002079"
     CDS             688..>1658
                     /gene="mafB"
                     /locus_tag="ACFB78_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC1269973.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVK"
BASE COUNT          444 a          609 c          375 g          230 t
ORIGIN      
        1 ggctaccaca acaaccccga cagcgccacc cgatacagct accccgccta tgacactacc
       61 gccaccacca aatccgacgc gctctccggc gtaaccactt ccacatcggt cttaaacgcc
      121 cccgccgccg ccctgacgaa aaacaacgga cgcaaaggcg aacgctccgc cggactgtcc
      181 gtcaacggca cgggcgacta ccgcaacgaa accctgctcg ccaacccccg cgacgtttcc
      241 ttcctgacca acctcatcca aaccgtcttc tacctgcgcg gcatcgaagt cgtaccgccc
      301 gaatacgccg acaccgacgt attcgtaacc gtcgacgtat tcggcaccgt ccgcagccgt
      361 accgaactgc acctctacaa cgccgaaacc cttaaagccc aaaccaagct cgaatatttc
      421 gccgtcgacc gcgacagccg gaaactgctg attgccccta aaaccgccgc ctacgaatcc
      481 caataccaag aacaatacgc cctctggatg ggaccttaca gcgtcggcaa aaccgtcaaa
      541 gcctcagacc gcctgatggt cgatttctcc gacatcaccc cctacggcga cacaaccgcc
      601 caaaaccgtc ccgacttcaa acaaaacaac ggtaaaaacc ccgatgtcgg caacgaagtc
      661 atccgccgcc gcaaaggagg ataaaccgtg aaaccgctgc gcagactgac aaacctcctt
      721 gccgcctgtg ccgtagcggc ggccgcactc atacagcccg ccctcgcggc ggacttggcg
      781 caagacccgt tcattaccga taacacccaa cggcagcact acgaacccgg cggcaaatac
      841 cacctcttcg gcgacccgcg cggcagcgtt tccgaccgca ccggcaaaat caacgtcatc
      901 caagactata cccaccagat gggcaacctg cttatccaac aggcaaacat caacggcaca
      961 atcggctacc acacccgctt ttccggacac ggacacgaag aacacgcccc cttcgacaac
     1021 cacgccgccg acagcgcaag cgaagaaaaa ggcaacgttg acgacggctt taccgtgtac
     1081 cggctcaact gggaaggaca cgaacatcat cccgccgatg cctacgacgg cccgaagggc
     1141 ggcaattacc ccaaacctac gggcgcacga gacgaataca cctatcacgt caacggcaca
     1201 gcccgcagta tcaaactcaa tccgaccgac acccgcagca tccggcaacg catatccgac
     1261 aactacaaca acctcggcag caatttctcc gaccgcgccg atgaagccaa cagaaaaatg
     1321 ttcgagcaca atgccaagct cgaccgccgg ggcaacagca tggagtttgt caacggcgtc
     1381 gccgccggcg cgctcaaccc ctttatcagc gcgggcgaag ccttgggcat aggcgacata
     1441 ctgtacggaa cgcgctatgc catagacaaa gccgcaatgc gcaacatcgc ccccttaccc
     1501 gccgagggca aattcgccgc catcggcggc ttgggcagcg tggcgggctt tgaaaaaaat
     1561 acgcgcgaag ccgttgaccg gtggatacag gaaaacccca atgccgccga aaccgtcgaa
     1621 gccctggtca acgtcctgcc gtttgccaaa gtcaaaaa
//
LOCUS       DAXDZG010000140         1494 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXDZG010000140 DAXDZG010000000
VERSION     DAXDZG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.140"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..158)
                     /locus_tag="ACFB78_002080"
     CDS             complement(<1..158)
                     /locus_tag="ACFB78_002080"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFC1269974.1"
                     /translation="MALGTSANASNFGISLGKSSAASGTKGIAVGTSSQATNLSAVAI
                     GTESKAQN"
     gene            444..788
                     /locus_tag="ACFB78_002081"
     CDS             444..788
                     /locus_tag="ACFB78_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC1269975.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            742..1395
                     /locus_tag="ACFB78_002082"
                     /pseudo
     CDS             742..1395
                     /locus_tag="ACFB78_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          455 a          307 c          307 g          425 t
ORIGIN      
        1 ttattttgtg ccttactttc agtaccaatt gccaccgcac ttaaatttgt tgcctgagaa
       61 gatgtcccca ctgcaattcc tttcgtacca ctggcagcac tgcttttccc taaggaaata
      121 ccaaaattcg atgcattagc agaagtaccc aatgccactc aattttagat gccgatgctt
      181 tatcgttcca tgataaggag ccattattac cttcttgcaa agtagaatcc gccccataag
      241 aagacaaagc actcattaac aaaataccag ccgctatcgc ggacgaccta tatacataac
      301 attacttcat atcatttcat tcaatatcaa tagtatagga taactccccc ccccgtcaat
      361 cgaaataatt cccgtatttt aatgtcgtat tgttcctgta tatttagtga tgtgataagt
      421 atagtttatt ttttgaggtt tttatgaatt taccttttcg ggcaatggtg tctgatttag
      481 gtggcacact tttaacacct gaacatttag tgggtgatct taccattgat actctgagag
      541 tattagagca aaaaggcgta gatattattt tggcaacagg acgcaatcat accgatatgt
      601 catccattct tggaaaaatc ggtgcggaac gtgcggttat gattacatca aacggtgctc
      661 gtgtacggga tttacagggt aatttgcttt atagcaatag tttgcctgaa gaattagttc
      721 tcgagcttta caaaacatca tatgtgggaa atcttattca gataccaaga ttttgtcgcc
      781 atcaataaac ctcaaggcat atccgtccac cgttccggcg gcgaagtcag tctgacggca
      841 acacttgcgg cacagttggg cgtagaaaag gtatggttgc tgcacaggtt ggacaagcag
      901 gccggaggca tattgctgtt tgcccttaat ccccaaagtg ccgctgttct tgccgcacag
      961 tttgccgaaa ggaaaatgaa gaaaacctat ttggctttgt ccgaccgcaa accgtccaaa
     1021 aaacaaggat ggataaaagg cggcatggaa aaatccaggc gcgggatgtg gaagctgacg
     1081 cgcaatatgg aaaatattgc cgttacccgg tttttcagta tccgcatcag tgaaaaaatg
     1141 cggctgttca tccttgagcc acatacgggt aagacacatc agttgagggt ggtaatgaag
     1201 agtctgggca gctctatatt tggggacagc ctgtatggag gaacagaatc cgaaactatg
     1261 ttcctgtatg cgtgaaaaat acaatttacc tatcaacaac aagaaatcga aattaccgca
     1321 cctttgaaaa atgcatggca ggccgaaaat atctctcatg cactggaaga attttatatg
     1381 gcaaaagcag gttaatagat ataaatcaat taaaacaata tgataaatat ttttataaaa
     1441 ttttttcaaa aaacggttga ccgcaaatct ctttaagcgt atagttcagt tcgt
//
LOCUS       DAXDZG010000141         1359 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXDZG010000141 DAXDZG010000000
VERSION     DAXDZG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.141"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..437
                     /gene="mafB"
                     /locus_tag="ACFB78_002083"
     CDS             <1..437
                     /gene="mafB"
                     /locus_tag="ACFB78_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1269976.1"
                     /translation="KWDWVKNTGYEKPAARPMQTVDGEMAGGNKPIKSLPNSAAEKRK
                     QSFKKFSSNWSSASFDSVHKTLTPNAPGILSPDKVKTRYTSLDGKITIIKDNENNYFR
                     IHDNSRKQYLDSNGNVVKTGNLQGKQAKDYLQQQTHIRNLDK"
     gene            434..790
                     /locus_tag="ACFB78_002084"
     CDS             434..790
                     /locus_tag="ACFB78_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC1269977.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILTVKEKLQNSDFSTEPLVKNIIH
                     VFEYAIKNEKTVIFHF"
     gene            964..1338
                     /locus_tag="ACFB78_002085"
     CDS             964..1338
                     /locus_tag="ACFB78_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC1269978.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFKNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT          509 a          186 c          248 g          416 t
ORIGIN      
        1 ccaaatggga ttgggttaaa aataccggct atgaaaaacc tgctgcccga cctatgcaga
       61 ctgttgacgg ggaaatggca ggtgggaata aacctattaa atctttacca aacagcgccg
      121 ctgaaaaaag aaaacaaagt tttaagaagt ttagtagcaa ctggagttca gcaagttttg
      181 attcagtgca caaaacacta actcccaatg cacctggtat tttaagtcct gataaagtta
      241 aaactcgata cactagttta gatggaaaaa ttacaattat aaaagataac gaaaacaact
      301 attttagaat ccatgataat tcacgaaaac agtatcttga ttcaaatggt aatgttgtga
      361 aaaccggtaa tttacaaggt aagcaagcaa aagattattt acaacaacaa actcatatca
      421 ggaacttaga caaatgaatg aacacaacct gttaattttc tgtttaaaag acaatgtttc
      481 aattagtgaa tatactgaaa tggttgattg ggcttatgaa aacattcaat ctgaaacagt
      541 tgtagaaatt acggaaaatc aaattattga atatcaaaat cgtggattat gggggcttgt
      601 ttctgaaatt accgataatt ggttatttgg accaagtgag ggggattggc taatagataa
      661 ggaaagtatt ttgactgtaa aagaaaaatt acaaaattca gatttttcta cagagccctt
      721 agtgaaaaat attattcatg tatttgaata tgctataaaa aatgaaaaaa cagtaatttt
      781 tcatttttga aactaatcta atttttagta atattgatgc agagcaagca gcattagatg
      841 ccgcaaacat ggggagaagc tattcaattt agaattaaaa aaacaaagtg aaaatggact
      901 agcaccacca aattggtcta cccagtttcc taatggtagt atttatgata ctaaggtaac
      961 gaaatgatta ttcaaaatga atttaattta tatcctagta atatgcttcc tgaagggttt
     1021 tgttatcctg aaaagtatgt tcgtatctct aacgatacat ctttaatacc ttatattcag
     1081 ccacataatt ttcactggtg gtttgagaat tatggaacag aaggggcaga agtagcttat
     1141 atatttaaaa attccatcct gcctgattta aatcttatcc cattcgctag taatggagaa
     1201 tgggaagctt attttgatgg taatgatgta acaggaaatc ctagggttat tgttattaat
     1261 ttagataata tagaaaacca tgaatttttt aatagttttg aagagtggct tgaattagca
     1321 attaaggata cttggtaagc agctatctat aaagagatg
//
LOCUS       DAXDZG010000142         1341 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXDZG010000142 DAXDZG010000000
VERSION     DAXDZG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.142"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(109..1032)
                     /gene="lpxC"
                     /locus_tag="ACFB78_002086"
     CDS             complement(109..1032)
                     /gene="lpxC"
                     /locus_tag="ACFB78_002086"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC1269979.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          321 a          332 c          365 g          323 t
ORIGIN      
        1 acggaaactt atcgggtaaa acggtttctt cagattttac gtcctagatt cccgcccgcg
       61 cgggaatgac ggcatctgtt tgacggcatt tgtttggaaa agggggcgtt atccgcaatt
      121 tctgatgttc agctcgtgaa atgcgtcggg caaatcatcg ctgtcggcaa attccaccca
      181 ttcgtaagcc gtttcgtctg ccaaaactgc gcgcaaaagg gcgttgttga cggcgtgtcc
      241 cgatttgtag ccttcaaacg cgccgacaat cggatgtccg acgatataca aatcgccgat
      301 ggcgtcgagg attttgtggc gcacaaactc atcgggatag cgcaagcctt caggattcag
      361 gacatccgtg tcgccaatca cgatggcgtt gttcaaattg ccgcctaagc cgagattgtg
      421 ggcgcgcatc atttccactt cgtgcataaa gccgaaagtg cgcgcgcgcg cgatttcgcc
      481 gatgtaggat ttgccggcga aatcaatttc aaacgtaggc gggctgcggt tgaaaacagg
      541 atggtcgaat tcgatggtca gcgttacctt gaaaccgtca tacggcgtaa atcggaccca
      601 tttgcccgct tctttgattt cgacgggttt gaggattttc aaaaaacgct tttgcgcctt
      661 ttggtctaca acgcccgcat cttgcaggag gtaaataaac ggcaggctgg agccgtccat
      721 aatcgggatt tcgggcgcgt tcaactcaat cagcgcattg tcgatgccgt aggcggacag
      781 tgcggacatg atgtgttcga tggtgccgac gcgcaggcct ttgtcggtaa cgatggtgga
      841 tgaaaggcgg gtatcgttga tcaaataagg gttcagcttg atttgttcgc ccatctcgcc
      901 gtccaaatcg gtgcggcgga aggaaatccc gctgttttca ggcgcggggt gcagggtcag
      961 cgcgacgcgt tcgcccgaat gcagcccgac tccggtaacg ccgatggatt tcgccaaagt
     1021 tctttgcagc ataaaccgct tccttatcaa tggggtaagt ttcggaataa tacgataaaa
     1081 ccggaaaaac aggctatgtt tttcaatagt atttgccaat gtatccgttt ccaacacgta
     1141 agccgcatga aaatgtatgg cggattaacg aaaatcagga caaggcggcg aagccgcaga
     1201 cagtacggca aggcgaggca acgccgtact ggtttaaatt taattcacta tacatcccgt
     1261 cattcccgcg cgggcgggaa tccaggacgt aaaatctcaa gaaacctttt ttcccgataa
     1321 gtttccgcac cgaccggcct a
//
LOCUS       DAXDZG010000143         1340 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXDZG010000143 DAXDZG010000000
VERSION     DAXDZG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.143"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..620
                     /locus_tag="ACFB78_002087"
     CDS             <1..620
                     /locus_tag="ACFB78_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1269980.1"
                     /translation="TGYKPVTARYGNPYRETVYIEVSDGIGNSQTLISNRIHPFYSQG
                     KWIQAGRLKKGDTLLSESGAKQTVQNITLKQQPLKAYNLTVADWRTYFVKGNQAETEG
                     VWVHNDCPYGNLSDNKSVGEGKKFTPAQKKAIIQENMNRNGGVVKSDQSGEVLVRPKK
                     SQKGITPPSNEWQIDHVQAKSKGGSNSYKNAQVLSRRENIKKSNK"
     gene            629..901
                     /locus_tag="ACFB78_002088"
     CDS             629..901
                     /locus_tag="ACFB78_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269981.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIDEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            1068..1298
                     /locus_tag="ACFB78_002089"
     CDS             1068..1298
                     /locus_tag="ACFB78_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC1269982.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          487 a          236 c          265 g          352 t
ORIGIN      
        1 taacgggata caaacccgtt accgcccgat acggtaatcc gtatcgggaa accgtttaca
       61 ttgaagtttc agacggcatc ggcaacagcc aaaccctgat ttccaaccgc atccatccgt
      121 tttacagtca aggaaaatgg atacaggcag gtcgtctgaa aaaaggcgac accctgcttt
      181 ccgaaagcgg tgcaaaacag acggttcaaa acattaccct caaacagcag ccgctcaaag
      241 cctacaatct gaccgttgcc gactggcgta cttactttgt caagggcaat caggcggaaa
      301 cggaaggagt ttgggttcat aatgattgtc cgtatggaaa tttatcggat aataaatctg
      361 ttggcgaagg aaaaaaattt acaccagctc aaaagaaagc aatcatccaa gaaaacatga
      421 atagaaacgg aggggtagtg aagtctgacc aatctggaga agttttggtt cgtcctaaaa
      481 agagccaaaa aggtatcacc cccccatcaa atgaatggca gattgaccat gtgcaagcaa
      541 aaagtaaagg aggttcaaat agttataaaa atgcacaggt tttatccaga agagaaaata
      601 ttaagaaatc aaataaatag gtatcaatat gcaatggaaa tttgaagatc ctcaaaatac
      661 tgctgttatt accaccagaa gaattttgtc taaaaaaaac ggaatcttag aagtatggca
      721 tgatgctgat gatggaatgt ggcaattctt agatggcaat gaaattgatg aaggagatgc
      781 tgtgatagtt agtttaaaag aaattgtcac tattgattcc tcagtcaatc tcttaagtaa
      841 tttacctctt ggtggttacg cttggagaaa tactatatcg gaaaaatgga attttgatta
      901 gcaagcgtag gttaggttaa aaaaaccaac aatcaaaggt cgtctgaaac agtgtttagt
      961 ttttcaaacg accttttctt caattaacat agaatgttgg gtctcgaccc aacctacacg
     1021 cttactaatc tcaaatataa ccttatgaaa taatgagtat ttaaaagatg gaaacattag
     1081 acgaacgaat aaagaattta ggaaaatctc ttgaagatag aattgacgct aatctaattg
     1141 atgccacact agagtacatt actttttcag aacgtttgct agcttttgaa acgctatgtg
     1201 attatattga agatttcaat atccagctta cggaaaaaga atcccaagaa atctctttta
     1261 ttaataagga atttggaatt gaaagtacat cagattaata aatattaggt cggtttaaaa
     1321 acccaacaat cacaaggtct
//
LOCUS       DAXDZG010000144         1322 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXDZG010000144 DAXDZG010000000
VERSION     DAXDZG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.144"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(12..290)
                     /locus_tag="ACFB78_002090"
     CDS             complement(12..290)
                     /locus_tag="ACFB78_002090"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC1269983.1"
                     /translation="MAISDVFYQKQLKGNGLVQSLSRKGNCLDNAAMESFFGTLKSEC
                     FHTCKYDSVTESEAALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="ACFB78_002091"
     CDS             complement(254..832)
                     /locus_tag="ACFB78_002091"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC1269984.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..>1322)
                     /locus_tag="ACFB78_002092"
     CDS             complement(837..>1322)
                     /locus_tag="ACFB78_002092"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC1269985.1"
                     /translation="VLPAARRLVLIFVNSLYFSISDSLVRKWVARYRLHGESGIKRRK
                     HTTKYSVEYKLEAIRPVTGQGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKP
                     KGRKPVKKQYPPQTKKADYLKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQ
                     G"
BASE COUNT          292 a          335 c          304 g          391 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtga attaacaaaa atcaggacaa ggcggcgggc cgcaggcagt
     1321 ac
//
LOCUS       DAXDZG010000145         1274 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXDZG010000145 DAXDZG010000000
VERSION     DAXDZG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.145"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(1..155)
                     /locus_tag="ACFB78_002093"
                     /pseudo
     CDS             complement(1..155)
                     /locus_tag="ACFB78_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(260..475)
                     /locus_tag="ACFB78_002094"
     CDS             complement(260..475)
                     /locus_tag="ACFB78_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269986.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTSN"
     gene            complement(599..958)
                     /locus_tag="ACFB78_002095"
     CDS             complement(599..958)
                     /locus_tag="ACFB78_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC1269987.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            complement(955..>1274)
                     /locus_tag="ACFB78_002096"
     CDS             complement(955..>1274)
                     /locus_tag="ACFB78_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269988.1"
                     /translation="RLASAVKDWKTIQPNKKGEINFGIGSATRQEAEQLGKIWVGDGA
                     KPVSSPSCQGCMLSADGTRLYRPPTTKSNTPESLNPTGVQANFVTRSVDGKTLTNGHL
                     NIK"
BASE COUNT          382 a          258 c          195 g          439 t
ORIGIN      
        1 gcactgtctg taattgcccc aatccctact ccttgaaaac ccgttaaaaa aggattaacc
       61 gcaccttgga tgatgccgcg aatatcatcc atacggttag cccaccaatt tttatcgggg
      121 tcgttttccc atatcagttt tcctgcttca tcagcacagt taggattggc attgatgccc
      181 acccagtttc aggcagcctg aaaaggattt ttaagatacg gctaggtggg ctgcgagaac
      241 ccacactctg cataaccatt tagttagatg tatccattag attatatgct tcaattaaat
      301 gttgttctag ctcttgcact aattgaggat tgcgaattcc tgacacaatc tcaaattctt
      361 ctgcatctat gcaaacacct gcataaattt caataacaag ggaacgcaat aattgaagct
      421 cttctcttgt taaagaaata ataatgtcat cacctgtgta attgattata ttcataataa
      481 ttttattttt gcttgtcaaa gtaagtttta cctaaggttg gtctaaatgc agtcccacca
      541 tcttttgaat ttgggtctct aattacaatt gctccagact tatcatccca aattgctctt
      601 atgtgtttgg attgtaatct tcgaattccc aagaaaaaaa tcgtaataag tttgaaagtg
      661 tcaaatccca agtttctttt gagcaatatt ctaatatttt atcaatttca cttttaataa
      721 tcttatgatc aaactgttct aatattaatg cattagacca aaaaaaacct tctttattac
      781 aatgatggga aatccattta ggagaacaaa tgcaaagtga aaaaatagat gagccttgtt
      841 ctccttcgat tccgatatcc aaatctatcc atctatgaaa attatctgga atttcggggg
      901 taaatttttc aaaatcaata tcatataaat ttatgctttt taaatccaat ttaatcattt
      961 tatattcaaa tgtccattag ttaaggtttt tccatcaaca cttctagtaa caaaattagc
     1021 ttgcactccc gttgggttaa gtgattcagg ggtgtttgat tttgtagttg gaggacgata
     1081 tagccttgtg ccatctgcac ttaacataca tccttgacaa gatggactac taactggctt
     1141 agccccgtct ccaacccaaa tttttcctaa ctgttcagcc tcttgacgag ttgcagaacc
     1201 aataccaaaa ttaatttccc cctttttatt tggttgtatt gttttccaat cttttacagc
     1261 acttgctaac cttg
//
LOCUS       DAXDZG010000146         1266 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXDZG010000146 DAXDZG010000000
VERSION     DAXDZG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.146"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            61..>1266
                     /locus_tag="ACFB78_002097"
     CDS             61..>1266
                     /locus_tag="ACFB78_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269989.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKLN"
BASE COUNT          423 a          272 c          314 g          257 t
ORIGIN      
        1 cgtgtcaatg atttaagcat ataatgataa aaatcacata ccttcaaaat ggaaaaaccg
       61 atgaaacaaa agaagacagt tcagtgcatc ttacttggct tcgcggccgc ttctatgcac
      121 gcgcaagggg cggccgctgc aaacagtgga acgatagaga agacagacaa atacaccctc
      181 gtcctcgcaa aacaagggca agagaacaac tataccctta atggcggaac cgaagtcaaa
      241 cccttaaatt ccctcatcat tgccgccaac ggcggtacga acaacattac gataaaaggc
      301 aaattggcag acggtcctgc cgatgccccg ccgacgattg acaataactc gattgaaaga
      361 aatataaata aaaacggtta cacgtacgca tggcagaatt ggagcggcgc ggtgatgttg
      421 gtggatcaaa gctatgaggg agaaaataag gttacttttg aaaacgtaac catagccgcc
      481 cataacgcgc ctgccggcat tttgtctgat gataggcata aatccagtag cttggcgccg
      541 gcgatgcttg cctttaaagg gcgcaatacc atcaatatgg acgcggactc aaatgccaac
      601 tctagtaacg aaggtatcct attgcttaat aatggtgaaa aaatgggaga atatcgtctt
      661 gtttccgaag aaggctcgac gctgaatatc aacatcaaat caggaaaaga taaaggacag
      721 ggcattaccg ccaaccacta tggtaactca gacatcaact tcaacaaggc ttctccaaac
      781 atcaccacaa tggagttcaa gggcgatgta aacattaaaa tcgataggaa cggacaggaa
      841 gaggcggaaa gcaacggttt tggcttctat tcaagccgca aattaggcaa taagaagcag
      901 attccggaag gttctaagat ggaagcgatt ttccgtggga acgtcgatat cgttgcaacg
      961 ccggtttatg acgagcaagg caggcccaaa agcataggca gcgcgtttgc gattgacggg
     1021 aaatacagca aagtcgaggt agtcggcggg gaaggcaagg ttgtcaaaat caaaggcgat
     1081 attttcgcct acaacggcgg cagcgtgagc gtgaaccttg ccaacaaaga ctcttatttt
     1141 gaaggggaag cccatatcgg gaaaaggagc tttgccaaag ggaaagatat gtttgcctta
     1201 accgtggatg cggacggata tgaattaacc cccgatacaa agtcgattga aaagaaaaag
     1261 ctgaat
//
LOCUS       DAXDZG010000147         1194 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXDZG010000147 DAXDZG010000000
VERSION     DAXDZG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.147"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..284
                     /locus_tag="ACFB78_002098"
     CDS             <1..284
                     /locus_tag="ACFB78_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1269990.1"
                     /translation="RLSLAIHEGKKNFPIGTATYEEADRLGKIWVGEGARQTSGGGWL
                     SRDGTRQYRPPTEKKSQFATTGIQANFETYTIDSNEKRNKIKNGHLNIR"
     gene            289..648
                     /locus_tag="ACFB78_002099"
     CDS             289..648
                     /locus_tag="ACFB78_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC1269991.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            725..934
                     /locus_tag="ACFB78_002100"
     CDS             725..934
                     /locus_tag="ACFB78_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269992.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            1035..1194
                     /locus_tag="ACFB78_002101"
                     /pseudo
     CDS             1035..1194
                     /locus_tag="ACFB78_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          438 a          148 c          244 g          364 t
ORIGIN      
        1 caagattgag tctagctatt catgagggta aaaaaaattt tccaatagga actgcaactt
       61 atgaagaggc agatagacta ggtaaaattt gggttggtga gggtgcaaga caaactagtg
      121 gaggcggatg gttaagtaga gatggcactc gacaatatcg gccaccaaca gaaaaaaaat
      181 cacaatttgc aactacaggt attcaagcaa attttgaaac ttatactatt gattcaaatg
      241 aaaaaagaaa taaaattaaa aatggacatt taaatattag gtaattatat gagattaaaa
      301 ttaaaaaatt atgattgttc tgactttgat ttggaagatt tcccacaaga caagttagaa
      361 aatttttgta tacttttgac attatcaata ggttttgatg aaagtaatgg tgctgattat
      421 ttttatgttt atatatatag caccgaatgg ttactttcaa atatacatag accgatgtca
      481 ttaaaaaact caattgttac aaaccgtttt aatattgaac acatattaaa attgataaat
      541 gatatattgg aaatatgtaa ttcaacgtct gaagataagt ctatatcaaa tttggcaaaa
      601 tattttgact gggaatttga cgattataat ctaaatatcc aagactaaag atggtgggac
      661 agcatttaga ccaaaaacag gtaaacagta ttttgatgac aaattaaaat aaaaggaatt
      721 attcatgaaa ttaattgatt ttgaagggaa tttagttaaa attagtctcg ataaagatga
      781 gttgtatatt attcaagcaa ttgtaggaga aatttattca ggcgtatgcg tggattgtag
      841 agattttgag ataattcatg gtgttgaaaa aaataaagtt cttcttttgg ataaagaact
      901 taaaaaaata tatgatactt gggataaatg ttagctagat agggtgggca ctcgttgccc
      961 accttttgcc gtatattaaa aatccttttc aggctgcctg aaactgggtg ggcatcaatg
     1021 ccaatcctaa ctgtgcggat gaagcaggaa aactgatatg ggaaaacgac cccgataaaa
     1081 attggcgggc taaccgtatg gacgatattc gcggcatcgt ccaaggtgcg gttaatcctt
     1141 ttttaacggg ttttcaggga ttgggagttg gggcaattac agacagtgcg gtaa
//
LOCUS       DAXDZG010000148         1157 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXDZG010000148 DAXDZG010000000
VERSION     DAXDZG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.148"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            76..567
                     /locus_tag="ACFB78_002102"
     CDS             76..567
                     /locus_tag="ACFB78_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC1269993.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEEHIGRMTGKLKAAKAQNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNL
                     QIR"
     gene            619..834
                     /locus_tag="ACFB78_002103"
     CDS             619..834
                     /locus_tag="ACFB78_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269994.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            945..>1157
                     /locus_tag="ACFB78_002104"
     CDS             945..>1157
                     /locus_tag="ACFB78_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269995.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          394 a          249 c          306 g          208 t
ORIGIN      
        1 catcaacatc gaacaggcat acgccataca cgccgcgctt ggcgaagcgg ttgccgaata
       61 tgagggaggg gcacaatgac cgccctcaca ctctaccggt gcgcggcaga cgtacaggcg
      121 gcgcttgatt actacttcga cagcgaaacc gagcgcgaag acacgctgga agccgttatc
      181 gggcagttcg aggtcaaagc gcaatccgtt atcgcttata ttaaaaacca agaaatcacg
      241 gaaaaaatgc ttgaagagca tatcgggcgg atgaccggga agctcaaggc ggcaaaagcg
      301 caaaatcaaa gcctgaaaga ctacttggcg cgcaatatgc aggcggcggg cattaccgaa
      361 atcaaagcgg acgacggcac ttttaaagcc tcgttccgca aatccgaagc cgtcgtgatc
      421 ttagacgaag cacaaatccc cgccgaattt atgcgtgagg ccgtcaaaac cgaaccggac
      481 aaaaccgcca tcagaaaagc gattgaaagc ggtcggcaag tagcaggcgc gaagattgaa
      541 gggcggaaga atttgcagat tagataaacc gtagaagatg ttgacgacag catcccattt
      601 tgagttaagg agcaaaaaat gagctatttg gaagatgtaa aaaacgcatt aagggtaata
      661 gataacttat gcaaagaagc attaaaagag cctgaatcgt tagagggtta tatagacgaa
      721 attagggata aagcagacga agcggatacc tctttggaat ttctaaagga tgtaataaat
      781 tatggtatta gcgatttaaa aaatgtaatt gaggtgtttg aagattgcgt ttgatattaa
      841 tggaacaaga catgaactat caatttaaat tcggcgaccc cgtgagagat acagctttat
      901 gattgggcat tgagagaacg gcatcaaaaa atcgagaaaa caaaatgcaa acagtagcaa
      961 caaaaccgac ggcaaaacag atgcttgccg ccaaacgcgc ggcgaaggaa tcaacgcggc
     1021 aggaacgcgc cgtcaaacgc gcgggaacag taaaaaacgt tgaccggaac cggctgtccg
     1081 cccggtcaaa agcgcaaaaa gaaaacatcg cccggatgtt gtcgggcgca aaggtatcgg
     1141 aagacgaagc cctgacg
//
LOCUS       DAXDZG010000149         1148 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXDZG010000149 DAXDZG010000000
VERSION     DAXDZG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.149"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..465
                     /gene="mafB"
                     /locus_tag="ACFB78_002105"
     CDS             <1..465
                     /gene="mafB"
                     /locus_tag="ACFB78_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1269996.1"
                     /translation="PMQTVDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSI
                     SPTGTIQDRIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKI
                     LGYQTEHRRVRYDINNNIYVLANPKTFKIKTMFKPNLGKEYYDGEFKKDMGN"
     gene            431..637
                     /locus_tag="ACFB78_002106"
     CDS             431..637
                     /locus_tag="ACFB78_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC1269997.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            658..1017
                     /locus_tag="ACFB78_002107"
     CDS             658..1017
                     /locus_tag="ACFB78_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269998.1"
                     /translation="MMTESHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKSIKKTN"
BASE COUNT          447 a          177 c          203 g          321 t
ORIGIN      
        1 cctatgcaga ctgtagacgg ggaaatggcc gggaaaaata agccaccgaa accaagtacg
       61 cagcaacact ctacacactc tgataacaat atcggcttac ctgccccata tgttaaacct
      121 gatacatcta tttctccgac aggaacaatt caagaccgca tcagatggac aaaatccaag
      181 tttcctactg agaaatcttt aaatggacat ttcaaagctc atggaaaaga atttggcgat
      241 ataaccattg aagactacca aaaaatggcg tctgatttgt tatcaaaaca gacatcggac
      301 aagatattag gttatcagac ggaacataga cgagtgcgct atgatatcaa taacaatatc
      361 tatgttttgg ccaatccaaa aacattcaaa atcaaaacaa tgtttaaacc aaacttagga
      421 aaggagtatt atgatggaga attcaaaaaa gacatgggaa attgacggag aaatatggct
      481 acattgtcct gtttgcggaa ctgaagttat ggactatgat atctgtgacg tttgtcagtg
      541 gcaaaataca ggagaaacta atatagatgg tggtcctaat gaaatgacac ttgcggaggc
      601 gaaagaagct tacgcaaaag gcttaccaat cagataaata agcacctaga gaaaccaatg
      661 atgacggaat cccatggtta cccatcaaat aatttacctg ttaaaatcat aaatgatatt
      721 atttatgcaa cccagttagt cgaagatttg gttttaggaa aaataaaaat tgttgatttt
      781 ttaaaatcat ataacaattt ttattattgg cttggttttg atgagttacc tcaatctgag
      841 aaaataaaat ttctaaacta tctcaatata ttaagtattc ataaggaaat acaagataaa
      901 actgtgaata gggtttatac cgattgtttt gatattgata aattacattc gcttggcaga
      961 attacaacta atgaatgtat taatggtata aaaaaatcta tcaaaaaaac aaattagaat
     1021 ttgacaatat actcaaataa ctagcctatg ttttgtaaaa taaaaatatt cttgctgagt
     1081 gtagagccag agataaaaag atttgatagt agtttgcagt tgtagaatta tataaacaag
     1141 aaaatagt
//
LOCUS       DAXDZG010000150         1129 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXDZG010000150 DAXDZG010000000
VERSION     DAXDZG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.150"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(278..688)
                     /locus_tag="ACFB78_002108"
     CDS             complement(278..688)
                     /locus_tag="ACFB78_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1269999.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(692..>1129)
                     /locus_tag="ACFB78_002109"
     CDS             complement(692..>1129)
                     /locus_tag="ACFB78_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270000.1"
                     /translation="GNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAIT
                     DGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDVEKATLDAARYADKHNLWDSQNK
                     AKVPTNDVVGYTDGKPTHIINIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          342 a          200 c          175 g          412 t
ORIGIN      
        1 aaaatgtcgg attcgagaat ccgacctacg gttgctgaac aatgttttgt agaacttgct
       61 caaaatccat gtttcgccat tttcctttat atatacctaa tgcagcacgg gttacgattt
      121 cgtttaaatc ataaatagcc atttacgtcc tctgctaatt ttcagcgagt agattgggtt
      181 accaaaccca acaaaaaagc agcctgaaaa atttcagaat gcttgtttta tgttttttat
      241 caatgattta ggttttaatc cagcctgcgc ttgctagtta aacagtattt acataaaaat
      301 cttgcactga acaaacaagc gtatcatgtt catcatgaaa tgatgggcat aacgttaatc
      361 tacaaatgtt tacatttaaa gatatttttt cagtttcaat atttttaatt tgtaaggtta
      421 atacatgagg atatttgtga tatagctgat tttttaattg ttcgttgtct aatatagcaa
      481 aaggtaattt tgcacacaaa taaccaataa ctttgttttt atgaaaaaaa actatccaat
      541 tttcaattcc ttcatcccaa tcaaaagtaa aattcgttcc ttctatgata aatttagcac
      601 aagatattaa atctaactta acccaagtgg atttatacag actcaatgct gatgaagtta
      661 taatattatc taagttataa attttcataa atcaacgttc cttacctggt gttccatgca
      721 caggtctaat cccatcttta ttcggctttc tacgataaat gttaattata tgtgtcggtt
      781 ttccgtctgt atagccaact acgtcgttcg ttggaacttt tgccttattt tgactatccc
      841 ataaattatg tttatctgca tatctagcag catctaatgt agctttttcc acatcaacat
      901 ctgatttaaa taaacttttg ctatctatct tgtctagact agatgcatca ggtttgtgtc
      961 gtaccatact gagagggtca gtaatatatt tgccatctgt aatagctgat ccaccattat
     1021 aggtagtatg cgtataattt ttttgtggaa cgagactatt ttgcaaagat tgttgtgtag
     1081 aaacgccagc tttgccatta ggagtagctt gcttcgctgg cttattccc
//
LOCUS       DAXDZG010000151         1076 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXDZG010000151 DAXDZG010000000
VERSION     DAXDZG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.151"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..250
                     /gene="exp2"
                     /locus_tag="ACFB78_002110"
     CDS             <1..250
                     /gene="exp2"
                     /locus_tag="ACFB78_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein Exp2"
                     /protein_id="HFC1270001.1"
                     /translation="TLNLFVEGFWIIGLLQALAFTTIVDYCTESKHTNMLNNPACNDE
                     DWDNDMLNNPACNDEDWDNDMLNNPAYSWSMFNIHHSD"
     gene            454..1041
                     /locus_tag="ACFB78_002111"
     CDS             454..1041
                     /locus_tag="ACFB78_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC1270002.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNRIFRNMKPGSDTDVAT
                     NDDWLKDFWSEDSLIYDPAYSNLNCNIFHRSDDDI"
BASE COUNT          338 a          195 c          216 g          327 t
ORIGIN      
        1 gacattaaat ctttttgttg aggggttttg gataattgga ttacttcaag cactcgcttt
       61 taccaccatt gtagactatt gcactgaatc taaacataca aatatgctca ataatccagc
      121 gtgcaacgat gaagattggg ataatgatat gctcaataat ccagcgtgca acgatgaaga
      181 ttgggataat gatatgctca ataatccagc gtactcttgg tcaatgttca atattcacca
      241 ttcagattaa aaaagcagga gccttatact cctgctttcc gcagcaaagt tacggatgta
      301 caacccggta aaaaaatctc cacttcctat gggcctgcct tggtcttaga aaaaggtgag
      361 aatggcttaa ttaaaattca gccaagcaga ggcaatgaac gttggattac aaatgatagc
      421 attcaagtaa cctataaata aaggagaagt atcatgatcg gattcgtaaa atggtatagc
      481 tatgagaaag attttggctt tatctttggt gaagatggtc atgaatactt tttccgtaaa
      541 tccgccggtt cagatatacc aggacagata gtgaatttcg aaccatttga gcatagtcgt
      601 gggctgcgag cgcgaaaggt tagtaatata cataatcctg atcctcaaca gatccaaaaa
      661 ttgaaaacct tatgcaaaga ctttcagaaa gttgaaaagc aacccatgtc taaaaaatac
      721 aacttgcttt cggcagctat attatcgctg tgtttgacta tgttcataac actgggcttt
      781 ttctacatct tagccggctt ttttgatact gatagggaga ctaacatagt tttgctgttt
      841 gtcttccctg cggtaacttt ttccgtgctg acattctttc tgaataggat attcaggaat
      901 atgaaaccgg ggtctgacac tgatgttgcc actaatgatg attggttaaa ggatttctgg
      961 tctgaggatt cattaattta tgacccagct tattccaatc tcaattgcaa tatatttcat
     1021 cgatcagatg acgatatata agaaagagga ttggtagctg aaatgctgcc aatcat
//
LOCUS       DAXDZG010000152         1018 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXDZG010000152 DAXDZG010000000
VERSION     DAXDZG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.152"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..372
                     /locus_tag="ACFB78_002112"
                     /pseudo
     CDS             1..372
                     /locus_tag="ACFB78_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            560..1015
                     /locus_tag="ACFB78_002113"
     CDS             560..1015
                     /locus_tag="ACFB78_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270003.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEIFKQLFHYPKLNDID
                     KCYLSNESYPTYIYDFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          362 a          202 c          195 g          259 t
ORIGIN      
        1 gaccgcgtct tcgccaaaga tgaaaccagt ggaaaaactg gatacaaacc tgttaccgcc
       61 cgatacggca atccgtacca ggaaaccgtt tacattgaaa tttcagacgg catcggtaac
      121 agccaaaccc tgatttccaa ccgcatccat ccgttttatt cgggcggcaa atggattaaa
      181 gcggaagatt taaaggcggg aagcagactg ttatccgaaa gcggcagaac ccaaaccgtc
      241 cgcaacaccg ttgtcaaacc aaaaccgctc aaagcctaca atctgaccgt tgccgactgg
      301 catacttact tcgtcaaggg taatcaggcg gaaacggaag gggtttgggt tcataattct
      361 tgtccgccta aaagaacagg aagctccaag aatgaaaaac atggagacgg cggtcgaagt
      421 caaatatcag cggaatcaaa aattgctgaa ttaacaaata aaattattcc cggaatgtcc
      481 aaaaatgaac gcttaaagat taagcaaaaa atcaaaaata ttgccaaaaa tgccaatcga
      541 aaaacaaaag gagaagagca tggtcgacgc ggtcgttaaa actcccgaat ttttaccttt
      601 tacttctgta ggaatattcc gttttggtgc tgatataaca cagtacaaaa acatactgga
      661 aacttttatg tatgaacctc ctgatgagtt tggaacagaa tattatgaat ctcccgactc
      721 aaatttactc atttccgtca aaaaaaacaa aattatttcg atattttgtt atcaggagct
      781 atattttatg ggcgttaata taataggtct aaattttgaa atttttaaac aacttttcca
      841 ttaccccaag ttaaacgata ttgataaatg ttatttatcg aatgaatctt atccgacata
      901 tatctatgat tttgatgaaa taggagtaca agcatgggaa gcaaaaggga aaattgttac
      961 tatcattgcc gggggcaaag acaattattc gacagaacct tactacgatg aataacat
//
LOCUS       DAXDZG010000153          959 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXDZG010000153 DAXDZG010000000
VERSION     DAXDZG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.153"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..309
                     /locus_tag="ACFB78_002114"
     CDS             <1..309
                     /locus_tag="ACFB78_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC1270004.1"
                     /translation="VWDDEEYWKLENALIEVRKKYPYPMDIPRDIVIGIGTIIDFLMV
                     QNWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDVEDWKFFYFPIQHSKGRLR
                     "
     gene            340..717
                     /locus_tag="ACFB78_002115"
     CDS             340..717
                     /locus_tag="ACFB78_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC1270005.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            815..959
                     /locus_tag="ACFB78_002116"
                     /pseudo
     CDS             815..959
                     /locus_tag="ACFB78_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
BASE COUNT          312 a          153 c          208 g          286 t
ORIGIN      
        1 gtgtgggatg atgaagaata ttggaaattg gagaatgctt taatcgaggt tagaaaaaaa
       61 tatccttatc cgatggatat accaagggac atcgtgattg gaatcggtac cattattgat
      121 tttttaatgg ttcaaaattg gaaacttttt gaaattaaag cttctccttg gttgcctaaa
      181 agtgtcaaaa ttaatgagcg ttatgaaagg ttcagagtaa tgctccgtta tatttttact
      241 gatctagatg tagaggactg gaaatttttt tactttccaa tacaacatag taaaggtaga
      301 ttgagatgaa aagtttaatt atattggaga tacattatta tgtgtgagtt caaggatatt
      361 ataagaaacg ttccttattt tgaggggtat gacgaaaatt catttattgg caaatggtat
      421 gatgacgggg tgtgggatga tgaagaatat tggaagttgg agaatgattt aatcgaggtt
      481 aggagaaaat atccttatcc gatggatata ccaagggata tcgtgattgg aatcggtacc
      541 attattgagt ttttaatggt tccaaattgg aaactttttg aaattaaagc ttccccttgg
      601 ttacctgata gcgtgggaat taacgaacgt tatgaaaggc ttaaaacaat gctccgttat
      661 atttttaccg agaaagacat agtcaacgtg caatttgatt attacaacaa aaaatagcaa
      721 aacccggcaa tcaaaatgcc gtctgaaacc gtgtttaatt ttcaggcgac cttcccctta
      781 ttttaaacag gatattggga actaagttct tcaaaaatcc tacacctgct ctttccacgg
      841 cagcaccttg gtcagaacgg cagacggcta caaagccatc gcccatattc aagccggaga
      901 ccgcgtcctt tccaaggacg aggcaagcgg gaaaacagga tacaaacccg ttaccgccc
//
LOCUS       DAXDZG010000154          928 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXDZG010000154 DAXDZG010000000
VERSION     DAXDZG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.154"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..526
                     /gene="mafB"
                     /locus_tag="ACFB78_002117"
     CDS             <1..526
                     /gene="mafB"
                     /locus_tag="ACFB78_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1270006.1"
                     /translation="GYETTVTRKTLDGQIAGKNKPIKPSSGDYVLPVGRQIIDPTRTS
                     FSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVDVVNMPDGAPTSMDNTRIMAARE
                     AGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKGVPEGWSKR
                     FPNGSIYDVKVLRK"
     gene            523..894
                     /locus_tag="ACFB78_002118"
     CDS             523..894
                     /locus_tag="ACFB78_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC1270007.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          322 a          151 c          192 g          263 t
ORIGIN      
        1 cggctatgaa acaactgtta cccgaaagac tttagatggg caaatagctg gcaagaacaa
       61 gcctattaaa ccttcttcag gagattatgt cttgccggta ggcagacaaa tcatagatcc
      121 tactcgtaca tctttttcac aagctacagt tagctatcag aagcgtggtg caaattacaa
      181 ctatgattct cttgtagctg caatgaacga aaaaaaatca tgggttggcg acagggttga
      241 tgttgttaat atgcctgatg gagcacctac tagtatggat aacacgcgta ttatggcagc
      301 acgtgaagca ggggtaaaag tggaagcgaa tgttcataat tttaatgacc gattatcatc
      361 aaaagagaga atcaggttta agcatgatgg tattgagcct caaacttggg gagaagctat
      421 ccagctacga attagaaagc aagaaacaca aaaaggagtt ccagaagggt ggagcaaaag
      481 atttcctaac ggaagtattt atgatgtaaa ggtacttagg aaatgataaa acaaaatagt
      541 tttgttccgt atcctgaagc aatgcttcct aaaggattta aatatccgca aagttattta
      601 aaattagctc aatccactca tgccattaac tacgatgaac aatattcttt tccttggtgg
      661 tttgaaaatg cagaaagcaa tatatcagaa gtaattgaca tttattttga aataactggc
      721 attccaaacc tattaccttt tgctagaaac caagagtggg ctgcctgttt tgatatttca
      781 gataaatcag gtaatcctaa aattatagta gttaatttag ataatacaaa atattacgag
      841 acttttgaaa attttgatac ttggctaaaa gaagctgaaa atgatggttg gtagcagccg
      901 taggtcggat tctcgaatcc gacatttt
//
LOCUS       DAXDZG010000155          884 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXDZG010000155 DAXDZG010000000
VERSION     DAXDZG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.155"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..444
                     /locus_tag="ACFB78_002119"
     CDS             <1..444
                     /locus_tag="ACFB78_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC1270008.1"
                     /translation="GDFSISYKNFSTVKPKVIAKGTINGKTFRDVNQSAKIGSPDSPT
                     LIAQRVNAKIQADGKPRPNATVANSHAEIGVIQQAYNAGETKGASMTMTVSGKDVCGY
                     CKGDIAAAAQASGLKSLTVNATDNVTGKNKTYYWTPGMKSIKERK"
     gene            441..857
                     /locus_tag="ACFB78_002120"
     CDS             441..857
                     /locus_tag="ACFB78_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270009.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTENYSGNTME
                     ILGDVYPIEHISKNISIIQDIISEFIMKNKPITIMI"
BASE COUNT          339 a          130 c          154 g          261 t
ORIGIN      
        1 ggggattttt cgattagcta taaaaacttt tctactgtca aaccaaaagt aattgccaaa
       61 ggaacgatta atggaaagac ttttagagat gttaatcaaa gtgcaaaaat tggatcgcct
      121 gattcaccta cattaatagc ccaacgagtt aatgcaaaaa tccaagccga cggtaaacct
      181 cgtccgaatg caaccgttgc taattcacat gctgaaattg gtgttatcca gcaggcttat
      241 aatgcaggtg aaacgaaagg ggcatctatg acaatgacag tttcaggaaa agatgtttgc
      301 ggttattgta aaggagatat tgcagctgct gctcaagctt ctggactgaa atcactgact
      361 gttaatgcga cagataatgt aacaggaaaa aataaaactt actattggac gcctggtatg
      421 aaatcaatta aggagagaaa atgaaaacat caacaattgt ttttggtgga ttttttatta
      481 cagataatgg agaacgaatc caaatcccta ttttagaaaa tcctaacatt aaagaaataa
      541 ataacttttt ttctgtatca aattttgaaa aaaaagccgg agtccttgtt ttcagaatta
      601 ttcctgagcc tgaatttggc aatactgaat taactatcta ttttgaaaaa ggatattatt
      661 taccaataat tcaaacaatt ttagaagatg gagatattga agtaaaaaat cttaaaacgg
      721 aaaattatag tggaaataca atggaaattt tgggagacgt ctaccctatt gagcatatct
      781 ctaaaaatat ttctataatt caagatataa tttctgaatt tattatgaaa aataaaccaa
      841 tcacaataat gatttaattg aaaagttcaa caatcaaaag gtcg
//
LOCUS       DAXDZG010000156          875 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXDZG010000156 DAXDZG010000000
VERSION     DAXDZG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.156"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>875
                     /locus_tag="ACFB78_002121"
     CDS             <1..>875
                     /locus_tag="ACFB78_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC1270010.1"
                     /translation="GLDKRKFNARELAEGQIMSAPLRDVIPEGAVLIVGEAHYTYPVR
                     AAGRPVPPYIQELTELRHHGHTVILMTRHPSQLDIFVRNLVSKHVHLERKAIGMKQYY
                     WYKCVTSLDNPAGVSGVEAANWKPPKEAFKYYKSASRHQKFKKKVPWAVWALIAVVGF
                     VGWKSYGMFQVYSKATDSRIEQEAQKESVVQTMTEQTASSETAPFEHSDNLKPEDFVP
                     TLPEKPESKPIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVK
                     NGLPFNPYKDERQRT"
BASE COUNT          271 a          208 c          220 g          176 t
ORIGIN      
        1 acggattgga caaacgaaaa ttcaatgccc gcgaactggc ggaagggcaa atcatgtccg
       61 ccccgcttcg tgatgtcata ccggaaggcg cggtgctgat tgttggcgaa gcgcactaca
      121 cttacccggt acgcgcggca ggccgtcccg ttccgcccta tattcaggaa ctgacagaac
      181 tccgccatca cgggcatacc gtcattttga tgacgcggca cccgagccaa cttgatatat
      241 tcgtccgcaa ccttgtttca aagcatgtac accttgaacg caaggcaatc ggaatgaaac
      301 agtattattg gtataaatgc gtaacctcgt tggacaatcc ggcaggcgtg agcggcgtag
      361 aagccgcaaa ttggaaaccg cctaaagaag ccttcaaata ctataaatcc gcaagccggc
      421 accaaaagtt caagaaaaaa gtgccttggg cggtttgggc gttgattgcg gttgtagggt
      481 ttgtaggctg gaaaagttac ggcatgtttc aagtttacag caaagccaca gacagccgga
      541 ttgagcagga agcgcaaaaa gaaagcgttg tgcagacgat gacggagcag acggcatcat
      601 cagaaacagc gccttttgag cattccgaca atctgaaacc tgaagacttt gtgccgactt
      661 tgcccgaaaa gcccgaaagc aagcctattt ataacacagt ccgacaagta aaaacctttg
      721 agcaaatcgc cggatgcata gacggcggaa aatcagattg cacatgctat tcaaatcaag
      781 gaacaccctt gaaagaaata acaaagataa tgtgtaaaga atatgtgaaa aacgggttgc
      841 ctttcaatcc ttacaaggac gaacggcaaa ggacg
//
LOCUS       DAXDZG010000157          858 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXDZG010000157 DAXDZG010000000
VERSION     DAXDZG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.157"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>858
                     /gene="tuf"
                     /locus_tag="ACFB78_002122"
     CDS             <1..>858
                     /gene="tuf"
                     /locus_tag="ACFB78_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC1270011.1"
                     /translation="ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAEL
                     LELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALDSYIPTPE
                     RAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMF
                     RKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRH
                     TPFFANYRPQFYFRTTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEEGLRFAIR
                     EGGRTVGAGV"
BASE COUNT          209 a          237 c          233 g          179 t
ORIGIN      
        1 atcctggtat gttctgctgc cgacggccct atgccgcaaa cccgcgaaca catcctgctg
       61 gcccgtcaag taggcgtacc ttacatcatc gtgttcatga acaaatgcga catggtcgac
      121 gatgccgagc tgttggaact ggttgaaatg gaaatccgcg acctgctgtc cagctacgac
      181 ttccccggcg acgactgccc gatcgtacaa ggttccgcac tgaaagcctt ggaaggcgat
      241 gccgcttacg aagaaaaaat cttcgaactg gctaccgcat tggacagcta catcccgact
      301 cccgagcgtg ccgtggacaa accattcctg ctgcctatcg aagacgtgtt ctccatttcc
      361 ggccgcggta ccgtagtcac cggccgtgta gagcgaggta tcatccacgt tggtgacgag
      421 attgaaatcg tcggtctgaa agaaacccaa aaaaccacct gtaccggcgt tgaaatgttc
      481 cgcaaactgc tggacgaagg tcaggcgggc gacaacgtag gcgtattgct gcgcggtacc
      541 aaacgtgaag acgtagaacg cggtcaggta ttggccaaac cgggtactat cactcctcac
      601 accaagttca aagcagaagt gtacgtattg agcaaagaag agggcggccg ccataccccg
      661 tttttcgcca actaccgtcc ccaattctac ttccgtacca ctgacgtaac cggcgcggtt
      721 actttggaaa aaggtgtgga aatggtaatg ccgggtgaga acgtaaccat tactgtagaa
      781 ctgattgcgc ctatcgctat ggaagaaggt ctgcgctttg cgattcgcga aggcggccgt
      841 accgtgggtg ccggcgtg
//
LOCUS       DAXDZG010000158          849 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXDZG010000158 DAXDZG010000000
VERSION     DAXDZG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.158"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..179)
                     /locus_tag="ACFB78_002123"
     CDS             complement(<1..179)
                     /locus_tag="ACFB78_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC1270012.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICMEATG"
     gene            259..488
                     /locus_tag="ACFB78_002124"
                     /pseudo
     CDS             259..488
                     /locus_tag="ACFB78_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(525..>849)
                     /locus_tag="ACFB78_002125"
     CDS             complement(525..>849)
                     /locus_tag="ACFB78_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270013.1"
                     /translation="HGKSPKCLGGNLGDFRGILQRSSGLYYKIWEGGWRMPALPFFRT
                     SIPMLVQNRMESIFNHNKDAIFLEGWSLGDAAMIMELLWQNISTSRRELTKLFLYKNF
                     WGCTR"
BASE COUNT          251 a          183 c          161 g          254 t
ORIGIN      
        1 tttcccgttg cctccatgca gatatgcaga ttctgacatc ccaagctttt caaccggtcc
       61 gaaaactgat ctaaaccttt tgaatcgttg tcaaactttg ccgaatattc ggcattgccg
      121 acaatggccg ttgcgtcaaa agtcagtttc gatatgtcca atcctacggt gttacgcatg
      181 ggattaccct tatatattgc gtccctaaga aggggcgatt aacaaaaatt aatgcccttt
      241 cttagggacg caatatataa ggtttatacc gtcgttgtcc ctaatgcgca atcagcgaca
      301 ttgttgccaa ttatccgaaa gaaagttaag cctgatggca ttgtatacac ggataccttt
      361 cgtagttatg atgtgcttga tgttagtgaa tttagccatt tacgtaagtt taacggcatt
      421 cccaaagagc atttggggct atatttaaag aaatgccaat ggcatttgaa atagaatctc
      481 aaatttagat tttaagacaa ttagttaatg ggaatttggt ctggttatct agtacaaccc
      541 caaaagtttt tatataaaaa cagtttggta agttctcttc gtgaagtgct tatgttttgc
      601 cacaaaagtt ccataatcat ggcggcatca cccaagctcc atccttccaa gaaaatggca
      661 tctttgttgt ggttgaaaat cgactccata cgattttgga caagcatagg gatagatgtt
      721 ctgaaaaacg gcagcgcggg cattctccaa ccaccttccc aaatcttata atacaaaccc
      781 gaagaccttt gcaaaattcc cctaaaatcc cctaaattcc caccaaggca tttaggggat
      841 ttcccatga
//
LOCUS       DAXDZG010000159          841 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXDZG010000159 DAXDZG010000000
VERSION     DAXDZG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.159"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>841
                     /gene="mafA"
                     /locus_tag="ACFB78_002126"
     CDS             <1..>841
                     /gene="mafA"
                     /locus_tag="ACFB78_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC1270014.1"
                     /translation="RLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARA
                     AVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYP
                     RYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNETLT
                     TNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETL
                     KAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDF
                     SDIR"
BASE COUNT          240 a          211 c          189 g          201 t
ORIGIN      
        1 cacggctgct gatacctatt cttttttcag tttttatttt atccgcctgc gggacactga
       61 caggtattcc atcgcatggc ggaggcaaac gcttcgcggt cgaacaagaa cttgtggccg
      121 cttctgccag agctgccgtt aaagacatgg atttacaggc attacacgga cgaaaagttg
      181 cattgtacat tgcaactatg ggcgaccaag gttcaggcag tttgacaggg ggtcgctact
      241 ccattgatgc actgattcgc ggcgaataca taaacagccc tgccgtccgt accgattaca
      301 cctatccgcg ttacgaaacc accgctgaaa caacatcagg cggtttgaca ggtttaacca
      361 cttctttatc tacacttaat gcccctgcac tctcgcgcac ccaatcagac ggtagcggaa
      421 gtaggagcag tctgggctta aatattggcg ggatggggga ttatcgaaat gaaaccttga
      481 cgaccaaccc gcgcgacact gcctttcttt cccacttggt acagaccgta tttttcctgc
      541 gcggcataga cgttgtttct cctgccaatg ccgatacaga tgtgtttatt aacatcgacg
      601 tattcggaac gatacgcaac agaaccgaaa tgcacctata caatgccgaa acactgaaag
      661 cccaaacaaa actggaatat ttcgcagtag acagaaccaa taaaaaattg ctcatcaaac
      721 ccaaaaccaa tgcgtttgaa gctgcctata aagaaaatta cgcattgtgg atggggccgt
      781 ataaagtaag caaaggaatc aaaccgacgg aaggattaat ggtcgatttc tccgatatcc
      841 g
//
LOCUS       DAXDZG010000160          815 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXDZG010000160 DAXDZG010000000
VERSION     DAXDZG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.160"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..472
                     /gene="mafB"
                     /locus_tag="ACFB78_002127"
     CDS             <1..472
                     /gene="mafB"
                     /locus_tag="ACFB78_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1270015.1"
                     /translation="TVWRGKKVKLNPTKWDWVKNTGYKTPAVRTMHTLDGEMAGGNRP
                     PKSITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHI
                     ENIVSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
     gene            482..688
                     /locus_tag="ACFB78_002128"
     CDS             482..688
                     /locus_tag="ACFB78_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270016.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
BASE COUNT          302 a          135 c          163 g          215 t
ORIGIN      
        1 tacggtttgg agaggtaaaa aagtaaaact taacccgacc aaatgggatt gggttaaaaa
       61 taccggctat aaaacacctg ctgttcgcac catgcatact ttggatgggg aaatggccgg
      121 tgggaataga ccgcctaaat ctataacgtc caacagcaaa gcagatgctt ccacacaacc
      181 gtctttacaa gcgcaactaa ttggagaaca aattagtagt gggcatgctt ataacaagca
      241 tgtcataaga caacaagaat ttacggattt aaatatcaat tcaccagcag attttgctcg
      301 gcatattgaa aatattgtta gccatccaac aaatatgaaa gagttacctc gcggtagaac
      361 tgcgtattgg gatgataaaa cagggacaat agttatccga gataaaaatt ctgacgatgg
      421 aggtacagca tttagaccaa catcaggtaa aaaatattat gatgatttat aggaaaaagc
      481 catgaatata ctatccataa ataatcaaaa ctcaactatt tcactaactc aagatgaagt
      541 ttttgtttta cgagctatct tgaatgagat atatgcgggc gtatgtgtag attcaagaga
      601 atttgaaaat gtatctggtg ttagaaaaca tgaagtagat aatttacaac aacagtttgc
      661 tggaatttat aaaaaaatga caacttaaca acccaaattt ttgtcagagc ctagtgcaaa
      721 ttacaactat gattctattg tagccgaaat gaaagaaaaa aatcatgggt tggcgacagg
      781 gttgatgttg ttaatatgcc tgatggagca cccac
//
LOCUS       DAXDZG010000161          807 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXDZG010000161 DAXDZG010000000
VERSION     DAXDZG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.161"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(187..504)
                     /locus_tag="ACFB78_002129"
     CDS             complement(187..504)
                     /locus_tag="ACFB78_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC1270017.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
BASE COUNT          240 a          170 c          146 g          251 t
ORIGIN      
        1 ctggcggtta ttttggcgtt gattcgggct tttgcgcccg tggggatgtg ctcgaaattt
       61 gccgcttctg tgaatttgga aaaactgtta gaacttgaat ctaatttaaa accaattgat
      121 tttaaattat tacttgattg aaatttaatt tgtgattttt ctaatcttac aggttcagaa
      181 taagaattac tcgaacatag aattaaaatc agaactatcg gtgtactctt gataaacgaa
      241 ttcatgaaca gtttttccgt tctcttctga ttttcttata aaaataacag actcatcaga
      301 aaaataaatc ttccaaatat tatgtgcgac cctcttattt aagaaataag agaagctctc
      361 taaaatatcg tactctttta cgttattaac aaattcttca aattctttct gagcaataaa
      421 agccatcgat tgcccaaaat actcgcttga cggctgatat ttataaagtg ccaactgcgc
      481 ctgcgtgata aacggcttgt tcatggttct gcctttcaaa ggttgttttg aaagcctgat
      541 tttgacacca taacttcatg cgctcaatcc ttaaacagaa ccgccccgat taatacgggg
      601 acggcaacgc cgagatagaa ataaaaatcc atcatttcaa aacctttttc agcagggaaa
      661 caaagtaaac ggacgcgagg acgccgaata ctatccggcc tgtttcaaga ccgctttgca
      721 ggttgtcttt cggactgcat tccgccaatg aaagccttag cggctgaccg tccgacatct
      781 tccacatgct gccgttatat tccggcc
//
LOCUS       DAXDZG010000162          798 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXDZG010000162 DAXDZG010000000
VERSION     DAXDZG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.162"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(355..714)
                     /locus_tag="ACFB78_002130"
     CDS             complement(355..714)
                     /locus_tag="ACFB78_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270018.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RLARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
BASE COUNT          216 a          185 c          157 g          240 t
ORIGIN      
        1 tgattttatt tgggttgcag gttttggtaa agattcctgt tgcgacccga atgtctgatt
       61 ttttttgggc gtatctcagt ccggaatcac tccgttagtg ggtttgcggt attgaaaaac
      121 agttcataaa aaaggaaaag gggggtattc gtaaagattg ggtaacgcac ccaatcttta
      181 caaagcttcc cccttttcct tttttccgcc ctattttcct gcacctacaa cccccgaacg
      241 aagcgattcc ggactgagac actttaaaaa aaacagccat tctagcagtt aacccccttc
      301 gctccgccca agccatcctg aggtgtagtg gctgaatttg tgattttggt tttatcaaac
      361 aaaatatttg actgaagtca catggcggtc gtcatatggg ggttccttcg cacccaaaaa
      421 atcgaccgcg taacggttta ctaaaacttc atcgtcctta aacttttggt gctttttccg
      481 gcaatatttt ctgaactccg ttaaatttga cggcaagaac ccgcaaccgt ctgcaccgta
      541 aatgtaatca acttcaaaca aactgtcttt ccgggcaagc cgaccatctt taagccagaa
      601 cataggctcg aaataaaaaa ctttcgacgt gtccgggaac ttatggagac ggagaacacg
      661 tgtgaaatcc ccgttttcat ctacaatttt gatatatctt ggtttgtgaa tcatgacatc
      721 ctcagattta gtattcagaa tatgatttta aaaagaactt tctgctttac gactccgccg
      781 ccgattcctt caaacggt
//
LOCUS       DAXDZG010000163          790 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXDZG010000163 DAXDZG010000000
VERSION     DAXDZG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.163"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(119..415)
                     /locus_tag="ACFB78_002131"
     CDS             complement(119..415)
                     /locus_tag="ACFB78_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270019.1"
                     /translation="MQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHP
                     RLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
     gene            complement(650..>790)
                     /locus_tag="ACFB78_002132"
     CDS             complement(650..>790)
                     /locus_tag="ACFB78_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270020.1"
                     /translation="GAVAGVGIDITPNLTLDAGYRYHNWGRLENTRFKTHEASLGVRY
                     RF"
BASE COUNT          149 a          180 c          285 g          176 t
ORIGIN      
        1 cggtgtatcg ggacggttcg ggtattcttg acaggatggg ttctcaagat gttgcgtagt
       61 gtcgggctcg caagggggtg catagagagg gttgcagggc gggcttcagc ccgccgcatc
      121 aaggtttttg gggaataggc gtgattcggc ggaattgatg gatttgacgg aatcggcggc
      181 aatggcggga tcggtaatat tgaagggatt ggtggaggcc ggcggcaacg gcgggggcgg
      241 aaaagacgcg ccagccgccg aaatcgtagc cgagggcgag tcgggggtgg acggagcggg
      301 tgcggatgtt tttgaaataa tcgcttaccg tgcttatttt gccttggttt gcaccggttg
      361 cttcgggata atcgcgggtg atgtgctcgt aggcgtaagc cagatccgcc tgcacatacg
      421 ggccgcgccc atggtcttca cccgccgcct gccaagcatc agggcgagca ggaggggttt
      481 gtattgcatg gttcggcttt cggaaaaaat cggataatgc cgaaggccgc gcgaaagcgg
      541 ccggatgttc ggattatact gtcagttatg ccgtctgaaa atgccgtttg cccgatcttg
      601 cgccttaaaa atgccgtctg aaggttcaga cggcatcgga atcgggggat cagaagcggt
      661 agcgcacgcc caacgaggct tcgtgggttt tgaagcgggt gttttccaag cgtccccagt
      721 tgtggtagcg gtagccggcg tccagggtca ggttgggcgt gatgtcgatg cctacgcctg
      781 ccactgcgcc
//
LOCUS       DAXDZG010000164          787 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXDZG010000164 DAXDZG010000000
VERSION     DAXDZG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.164"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..285
                     /locus_tag="ACFB78_002133"
     CDS             <1..285
                     /locus_tag="ACFB78_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1270021.1"
                     /translation="GNKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDG
                     VKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            282..599
                     /locus_tag="ACFB78_002134"
     CDS             282..599
                     /locus_tag="ACFB78_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270022.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
BASE COUNT          279 a          131 c          150 g          227 t
ORIGIN      
        1 ggaaacaagc cagttgttaa atctattaga ccaactacgc gagatgaatt acgtcaagca
       61 ttgcaagaac aaggttttag acgtactggt tcagatgcgg ctcaatatga aacatggaaa
      121 ggtcctgatg gcgtgaaaat agatattcgt ccaaatggag aggttataag aacccaaaga
      181 gtgccgcgaa ccgatggtgt acagggaaaa tatccgcaac gacaagatta tgaaggcaat
      241 ccattgccaa ataatcatca tcattctgga tattttgtca aatgaaaaaa aatatttttc
      301 acaatgtaag cctttatgaa ataatctttt ccgataatgg aaataccctt acattatctt
      361 ttacagatac aattgaaggt aattatttcg gatatatcaa atgcagtaat attttgaatt
      421 ttaaattaga tacaaataat ttcgtagatt atgaggataa ggaagatagc ttgtttccct
      481 tgtttatacc cgaaatagag ctatataaat accaatttta tagtgaaatt attattgatg
      541 tagggattat tataaaaata tctgctgaaa caattaattt tgagccactg ggaaaatagt
      601 aactgctttc ccagcagccg tagcaactgt atttttaccc gacggggtaa aaatacagtt
      661 gctacatctc tgcgcagcag actctacaag gtattaataa ttcaggaaaa ttaagtccgg
      721 aagcacaact tgccgccgtg agcctattac aggacagtgc ttttgcggta aaagacggta
      781 tcaactc
//
LOCUS       DAXDZG010000165          770 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXDZG010000165 DAXDZG010000000
VERSION     DAXDZG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.165"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(99..422)
                     /locus_tag="ACFB78_002135"
     CDS             complement(99..422)
                     /locus_tag="ACFB78_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270023.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(425..613)
                     /locus_tag="ACFB78_002136"
     CDS             complement(425..613)
                     /locus_tag="ACFB78_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFC1270024.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
BASE COUNT          197 a          134 c          106 g          333 t
ORIGIN      
        1 tatttttatt tccagtcatt ctggctattt tatgtatatt tccattatgt ttgattatca
       61 ttttattttt tccaaggatt ttcttctata ttgatagttt atctaaagat ttcatttaaa
      121 atatcctgac taatttcttg tttatataat tctacaattt tctgtttctg tgtatttgta
      181 taaaattttt cgttggtatc tgcttgatat tttaattgtc tcaatatttc catgatttca
      241 tattctgatt tttcatcatg ttcttcaatc atgtaatcaa caaattgttt tagaatatca
      301 aaggcttcgt aactttccaa aattttaata tgtttatcgg caaaattgga catataccat
      361 tcgtcagtga ttggattggt atttaaatca tctaaaaaaa catttacatc atctaaaaac
      421 atggctaatc cttaaacttg aaatctgttt tctcaccggt tcttgtattt tcaatgtaat
      481 gtatctcaac tttgtttata ttttgaacgc gcttaaccca gccgtctttt gccaaccaac
      541 catttttagt atcggacata ggaggtattc tcgcaggagt tttgccctgt gggtttgcct
      601 taacttcttc cattgctaat tgttccttta aattattagg tattgtacga ccggttgatt
      661 tttcaacaga ttgtgtccct atttttaatg atttgtttcc ccccagccat ttccctgtct
      721 acagtctgca tatggcgggc agtaggtttt tcatagccgg tatctttaac
//
LOCUS       DAXDZG010000166          764 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXDZG010000166 DAXDZG010000000
VERSION     DAXDZG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.166"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(123..425)
                     /gene="imm40"
                     /locus_tag="ACFB78_002137"
     CDS             complement(123..425)
                     /gene="imm40"
                     /locus_tag="ACFB78_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC1270025.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(441..>764)
                     /locus_tag="ACFB78_002138"
     CDS             complement(441..>764)
                     /locus_tag="ACFB78_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1270026.1"
                     /translation="GEMAGGNKPLESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFP
                     QSVDAFSENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQ
                     QLPEK"
BASE COUNT          228 a          144 c           98 g          294 t
ORIGIN      
        1 attaatacct tgtagagtct gctgagcggc tgtatctgat ccggtcagcg taggttaggt
       61 taaaacctag cattttcaac atcttgatgt tttactataa acctattctt gtgcttcctt
      121 tgttagataa atttcaaaac aaaagacacg tatgcattct ctaattttat ttgagataaa
      181 taatgcattg ctttgttgat actgttaaat aaattacttc cctcgtaata ccaattatca
      241 tatgtttgat aaaaataatt atctttcttg atataaatat ctccccccaa tacaagtatt
      301 ctttcattta taaataaatt taatatatct attgcagtgg ggacgggtaa tgcggcttct
      361 ctaattccaa ttcctatcaa agattcccct tgtctcgaat aaatttcaaa aatttcattt
      421 aacatattaa tctctatcta ctatttttca ggaagttgtt ggttagggac aaataatcta
      481 tgattaattt ttccatctgc ttctctgata tattcaaaat ttccatcttt tcctttataa
      541 gatccaggga tataaagttt atgccttaca atattatctc cgccaactat ttgtattact
      601 gttccatttt cagaaaaagc atctactgat tgcggaaaac catgataatc accattcgat
      661 gcttgcctta atactttctc tgtataaccg gtattttcaa aaaaattatt cgttgttaca
      721 gtattttttg attccagcgg tttattccca ccggccattt cccc
//
LOCUS       DAXDZG010000167          665 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXDZG010000167 DAXDZG010000000
VERSION     DAXDZG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.167"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(43..>665)
                     /locus_tag="ACFB78_002139"
     CDS             complement(43..>665)
                     /locus_tag="ACFB78_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC1270027.1"
                     /translation="QALDEGVPYVAVFEDDVLFGKDAEKFLAEDTWLEERFDKDSAFI
                     VRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISREAMRFFLDRFAVLPAE
                     RIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQNSMLGSDLEKDREQGRR
                     HRRSLKAMFDLKRALGKFGREKKKRMESQRQAELEKVYGRRVILFK"
BASE COUNT          186 a          191 c          104 g          184 t
ORIGIN      
        1 gtgtgtccat ttctgatttt aatcccctat attttacaca aactatttga acaatatgac
       61 ccgcctgccg taaactttct caagctccgc ctgcctttga ctctccattc ttttcttctt
      121 ttccctaccg aatttaccca aagcacgctt caagtcaaac atcgccttca acgaacggcg
      181 gtgtcttctt ccttgttccc tatctttttc caaatcgcta cccaacatac tgttttgact
      241 gagaaacttg gcataatgca attcttgggt acataaggcg ggactaacct gataaacagg
      301 catcccctcc ttatcaaaga aataagtaaa catcatcaaa tctaccgctt taatccgctc
      361 tgccggcaaa acggcaaacc tgtccaagaa aaaccgcatc gcctcacgcg aaatgatata
      421 gccagccgtc ccacaatgtt cgctctccag caaaggaaat gaccggtttt cataattcag
      481 gactttatcc ggtctgacaa taactttcgc aaacatcgtt tccaaacgga cgataaaggc
      541 ggaatcctta tcaaaacgct cttccaacca agtatcttcg gcaaggaact tttctgcgtc
      601 tttgccaaac aggacatcat cctcaaatac ggcaacatac ggtacgcctt cgtccaatgc
      661 ctgtt
//
LOCUS       DAXDZG010000168          640 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXDZG010000168 DAXDZG010000000
VERSION     DAXDZG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.168"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            83..310
                     /locus_tag="ACFB78_002140"
     CDS             83..310
                     /locus_tag="ACFB78_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1270028.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPRMKAGGRGKPVTHYSIVKNGAEV"
     gene            307..>640
                     /locus_tag="ACFB78_002141"
     CDS             307..>640
                     /locus_tag="ACFB78_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1270029.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDDGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEP"
BASE COUNT          175 a          147 c          204 g          114 t
ORIGIN      
        1 ggccgataaa gaaaaacccg cacggggcgg gtaatccccc tgaattgcag ggaagcggtt
       61 caggtaacgg cgaaaggcga ttatgaatca aaaacaaacg caatgcaaac aaattgtcga
      121 ttacatccgt aacaagggat gcatcacatc ccttgaggct tatcagaacc tgaaggtgac
      181 gcagcttgcg gcacggataa ccgacttgga aggcaggggc ttcgtgtttg ccaagccgcg
      241 catgaaggcg ggcggccgcg ggaagcctgt tacgcattat tcgattgtca aaaacggagc
      301 ggaagtatga gtgcgaggct gatggggatg gctttcaaaa cgggtatccc gagggggcag
      361 cgttttgttt tggtaaagtt gtgcgactgc gccaacgacg atggcttgtg ttatccgtcg
      421 caagaaacgc tggcggaaga tacgggcttc gccgaaaccg ccgtacggca gcatatcaag
      481 tggctgaagg ataacaattt catcaagtcc gcccggcggc agagggggcg ggagaggaag
      541 tccgacatct accgcatcaa cgtcgccctg cttgaaaaat gctatgcgga ggcggcaaaa
      601 cggaaggcgg cgcggcaggc aaaaatgtgg gaagaaccat
//
LOCUS       DAXDZG010000169          619 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXDZG010000169 DAXDZG010000000
VERSION     DAXDZG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.169"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(321..>619)
                     /locus_tag="ACFB78_002142"
     CDS             complement(321..>619)
                     /locus_tag="ACFB78_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC1270030.1"
                     /translation="FFPDILACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQDSAQCP
                     APVTFTITVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREI"
BASE COUNT          142 a          166 c          173 g          138 t
ORIGIN      
        1 ccgcgcttct cggctgtttg cggcttcgcc cgcaaagtgc ggtacttcgc ctgcctttgg
       61 ctaaagttgc ttaatcggaa aggtcttgcg ggggctagcc cccacacccc cagtctcact
      121 tgcgacgccg cgcgggcagg gggaacggcg caaaaggcgc gcgcctatca ccctgcccgc
      181 gggcagagtg tattcttcgg cagggcggca aggggtatcc aaaaagattt ataaagacga
      241 tgaagccgtc tttacaaatc tttctggacg tcctcccccc tgcctgaata aacgttactg
      301 tagcccaacg gttttgaccg ctatatctct ctagataccg tcctgataca gaaaaaggcg
      361 gcaaccgccc aagcaagggc gagaagcatg taccttagcc gttcggctat ggtacatgcg
      421 ttctcaaagc tgaacgcgaa ctgcttgctt gaatcaagca cggttatagt gaacgtaacg
      481 ggggcgggac actgtgcgga atcttgaaag attccggatt tctgaaactc tacattgacg
      541 gtttcagacg gcagatttaa atcttctgcc ggattgggct cgggcagcct gtcgcaagcg
      601 agaatgtcgg ggaagaatt
//
LOCUS       DAXDZG010000170          611 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXDZG010000170 DAXDZG010000000
VERSION     DAXDZG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.170"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..501
                     /locus_tag="ACFB78_002143"
     CDS             <1..501
                     /locus_tag="ACFB78_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270031.1"
                     /translation="ASKWQVRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELG
                     EFAETKELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVA
                     LDKVLMNAKYEGSFPNKLGRDHIIAVHKYSTGVRFVNKVLKIRIIAREKFDGIKHYDH
                     FILKDK"
BASE COUNT          188 a          112 c          162 g          149 t
ORIGIN      
        1 gcatccaaat ggcaggtgcg cggttgttcc cgttgtcggg cggacaggca agctggcgaa
       61 aggcagccgc gcgatggaaa cgggcgatgg acggcaggtg aggttgtttc cgtatcgggc
      121 ggcgaattgg gggaatttgc tgaaacaaag gagcttcgta aagcggctat gcagtatgcg
      181 cgtgataact ttgtcggcaa aagctatgtc aatgaaagca gcgggcatga actgaaggta
      241 acttggcaag gtgtgaaaca cgctgcgtca aaatcgaaca gcatcgaact ttcccttatg
      301 gtcgcgcttg ataaagtttt gatgaatgca aaatatgaag gaagttttcc taacaaatta
      361 gggagggatc atattattgc ggtacacaaa tactcaaccg gtgtcagatt tgtcaataaa
      421 gtcttaaaaa tcaggataat agcccgtgag aaatttgatg ggataaaaca ttacgatcat
      481 tttattttga aagacaaatg aaaaaaccct gtccagtata tctgggatag cttatggaag
      541 catttaaccc agcctcggat aaggctttaa atccatcatg ccatttatac ggtaaaaaca
      601 agggtctgta c
//
LOCUS       DAXDZG010000171          600 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXDZG010000171 DAXDZG010000000
VERSION     DAXDZG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.171"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            176..>600
                     /locus_tag="ACFB78_002144"
     CDS             176..>600
                     /locus_tag="ACFB78_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC1270032.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNK"
BASE COUNT          196 a          173 c          145 g           86 t
ORIGIN      
        1 gaccaagccc cgaagaaatc aaataccggc aaagcccgga aaccgccgaa acacggcgga
       61 tgaccgaaaa acaggcagaa ggccacatta aaagcattat caggtaacgc cgccgcgcaa
      121 ggcataaaac agcgcgagac cgatagacga aacgaccttt aacaggagac cgaaaatgtc
      181 attgatttta agtgtgaaag acgaaagcaa tttcaaacca tgcccggcag gcagccatca
      241 cgccacctgc atccgcatca tcgatttggg tacgcagctc gtcgagtacc aaaacgaaca
      301 aaagcggcag cacaagattt tagtgcagtg ggaaatcgac cctgaaggcg atccggaaat
      361 gctgatgccg gacggcaggc cctacctcat cagccgccgg tacaccgcca gcctgcacag
      421 caaaagccaa ctggcaacag acctcaaaag ctggcgcgga agggacttta caccggaaga
      481 acgcgacaac ttcgatttgc gcaatatttt gggcaagccc tgcctgttga gtatcgccca
      541 ccaagaaagc agcgacggca aaaccaccta tgccaacatt tccgccatca gcaacaagat
//
LOCUS       DAXDZG010000172          568 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXDZG010000172 DAXDZG010000000
VERSION     DAXDZG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.172"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            244..>568
                     /locus_tag="ACFB78_002145"
     CDS             244..>568
                     /locus_tag="ACFB78_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270033.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVT"
BASE COUNT          162 a          128 c          161 g          117 t
ORIGIN      
        1 tagggaggga tgtcatgata tttgaaaaat caataaacgg ctggaaatac cgcgccgtaa
       61 tggaaatcag aaccacgcaa aaggagcttg ccttgaacag cctttataca acagacagga
      121 ttgaatagtc atgacaaaac ctgcgacgag taactgtcaa tctttcgatt ccgtcctcat
      181 acatcctaag cgtctttgca aattctgttc agttatacca tttatacaga caagagcaaa
      241 ccaatgacga agttatacgc agaaatcgcc gagatggagg cgcaggacga cggcacggtc
      301 aaagtttggg gttatgcctc aagcgaggcg gtcgattcgg acggcgaagt catcgcggcg
      361 gcggctatga aggcggcgat tcccgattat atgaagttcg gcgaggtgcg cgagatgcac
      421 ggcagcaacg cggcgggaac ggcgattgaa atcaacgtgg aagacgacgg cagaaccttt
      481 ttcggggcgc atatcgtcga tcccgttgca gtggagaaag tcaaaacggg cgtttacaag
      541 ggcttttcca tcggcggcag cgttaccg
//
LOCUS       DAXDZG010000173          533 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXDZG010000173 DAXDZG010000000
VERSION     DAXDZG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.173"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..200)
                     /locus_tag="ACFB78_002146"
     CDS             complement(<1..200)
                     /locus_tag="ACFB78_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1270034.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEP"
     gene            328..>533
                     /locus_tag="ACFB78_002147"
     CDS             328..>533
                     /locus_tag="ACFB78_002147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270035.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRV"
BASE COUNT          159 a          103 c           92 g          179 t
ORIGIN      
        1 ggttcgctcg gaacggaatc caagtacaga ctgcccatcc catattcttc ttcaagccgt
       61 ctgcttacct tttcgccaaa agatgcttta ccgctcatca attgagataa aaggcttttg
      121 tctttaaccg gcaacttttt attttcaaag aatcttttta aattatcgat acggatggcc
      181 ttcagatctc tattagtcat tttcgcgctc cattcagatt gggattcaat tttatttagt
      241 ttctgctaaa caaacaaata ttaaattttc ttgcctatgc gtttaatatt tgctaaactc
      301 attaaatatt tacggtgaaa ctacagaatg actcattcag aatttattaa aacgctgtca
      361 gaacagcgtg gtgcaaaaac ggaatacgct aaaaaacttg gcttatccct gtcttttcta
      421 tggcagatag aaaacggacg ggcagtaacg ccaaagcgtc tttataaaga cgttatgttg
      481 ttgacaaaaa ataaggtttc tatttctgaa ttaatttcgg agttcagggt ttg
//
LOCUS       DAXDZG010000174          529 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXDZG010000174 DAXDZG010000000
VERSION     DAXDZG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.174"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT          117 a          143 c          147 g          122 t
ORIGIN      
        1 ggcgggaatc cagtccgttc ggtttcagtc atttccaaaa attgccgtag cgttaagttt
       61 ctagattccc actttcgggg gaatgacgcg gtgcaggttt ccgtgcggac ggattcgtca
      121 ttcccacaca gacaggaatc cggatttgtc cgcgcggaaa cttatgcgcc gtcattcccg
      181 cacaggcggg aattcaggtc tgtcggcata ggaacttatc ggataaaacg gttgccccaa
      241 ccctgcgttc tagattccca ctttcgtggg aatgacgatt caggtattcc taatagcaac
      301 ccgccacaac ccacctacgc ccatcctacg catctgccat acagccctgt tccggagcgc
      361 gcggataagg tgcgatgccg ccggcgggca tcatagtcct ctctccctgt gggagagagc
      421 tagaaagctg caaggcttgt attttagaag actaagggat ttgggaaaga ttgtcgaaat
      481 tcggggagcc ctctccccca caggggaggg ggcaggttgc ggcagattt
//
LOCUS       DAXDZG010000175          523 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXDZG010000175 DAXDZG010000000
VERSION     DAXDZG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.175"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT          132 a          138 c          117 g          136 t
ORIGIN      
        1 ctaaggcggg ccaacgccgt accggttttt gttaatccac tatacttata ccgttatgcc
       61 cgcgtccgga atttcagatt gcggacattt gtcaggaaaa acaaaaaacc ttccgccgct
      121 gcgcaggcga aaatcctatc cgttggaatc cggttgtttt aaataaatcc ttgcagcttt
      181 ggttttctgt ttttccgata acaccgtaac tttgaaacgc gtgaagcggg aatccgatcc
      241 gttgggattt tgcaacttca aatcaatccg caaaccggaa tcccgtcatt cccgcgaaag
      301 cgggaatcta gaacgcatcc gcacggaaac ccgcatcccg tcattcccac ggaagtggga
      361 atctagaatg tcaaatctca agaaaccgtt ttatccgata agttcctatg ccgacggacc
      421 tggattcccg cctgcgcggg aatgacggag gggaacggcg cacggacatt tgccggaatc
      481 ttttatgttt ccattatcat actcttttcg atgcgctatt cca
//
LOCUS       DAXDZG010000176          501 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXDZG010000176 DAXDZG010000000
VERSION     DAXDZG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.176"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..469
                     /locus_tag="ACFB78_002148"
     CDS             <1..469
                     /locus_tag="ACFB78_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270036.1"
                     /translation="IENVQKHDNGNRIDRKTENQENGTFHAVSSLGLSAVYDFKLNDK
                     FKPYIGMRVAYGHVRHGIDSTKKTKNTLTAYHGVGRGSTYYDNVDPEKNQKNTYRQNR
                     SSRRLGFGAMAGVGIDVAPGLTLDVGYRYHYWGRLENTRFKTHEASLGMRYRF"
BASE COUNT          143 a          145 c          115 g           98 t
ORIGIN      
        1 catagaaaat gtgcaaaaac acgacaatgg caacaggata gaccggaaga cggaaaatca
       61 ggaaaacggt acgttccacg ccgtttcttc tctcggcttg tccgccgttt acgatttcaa
      121 actcaacgac aaattcaaac cctatatcgg catgcgcgtc gcctacggac acgtcagaca
      181 cggtatcgat tcgactaaaa aaacgaaaaa tactcttacc gcctaccatg gtgttggcag
      241 aggatctacg tattatgata atgtagatcc ggaaaaaaac caaaaaaaca cttatcgcca
      301 aaaccgcagc agccgccgct tgggcttcgg cgcgatggcg ggcgtgggca tagacgtcgc
      361 gcccggtctg accctggacg tcggctaccg ctaccactat tggggacgcc tggaaaacac
      421 ccgcttcaaa acccacgaag cctcattggg catgcgctac cgcttctgat cccccgattc
      481 caatgccgtc tgaaccttca g
//
LOCUS       DAXDZG010000177          495 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXDZG010000177 DAXDZG010000000
VERSION     DAXDZG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.177"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..206)
                     /locus_tag="ACFB78_002149"
     CDS             complement(<1..206)
                     /locus_tag="ACFB78_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1270037.1"
                     /translation="MSEFKDRLKEARKNKNLSQENLAKLAEVSQSTIAALESGRNKKA
                     TNIAKLAKILDVSAFWLETGEGSR"
BASE COUNT          139 a           91 c          100 g          165 t
ORIGIN      
        1 tttcggctgc cttcgcctgt ctcaagccaa aaagccgata catctaaaat tttggcaagt
       61 ttcgcgatat tggtagcctt tttattccta cccgattcca acgctgcgat ggttgattga
      121 cttacttctg ccagttttgc caaattttct tgacttaaat ttttattttt ccgcgcctct
      181 ttcaggcggt ctttaaattc agacatatca aactccttat aacgcaacgg gtactattgt
      241 actttttatt ataagtattt tggtgcttgc attatgagta cgtttgtact tataatgttc
      301 aaaatttgag ctggggcaaa tatggacgaa ccaagagatt tagtgttgtt tttgaaagaa
      361 aaaatgagca gcgcggctat cgcgaaagag gtcggctgct cgaaagagtt tattaataaa
      421 atcggtaacg gcgagcgaaa aaacccgcgt tatcaaattg ttgattcttt aaggagttta
      481 tacaggaaga atcaa
//
LOCUS       DAXDZG010000178          471 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXDZG010000178 DAXDZG010000000
VERSION     DAXDZG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.178"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            133..>471
                     /locus_tag="ACFB78_002150"
     CDS             133..>471
                     /locus_tag="ACFB78_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC1270038.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSK"
BASE COUNT          138 a          112 c          112 g          109 t
ORIGIN      
        1 aactccgccc catccgtaga ggctgataac agtcatgagg ggtatggata cagcgatgaa
       61 gcagtgcgac aacatagaca agggcaacct tgattcacac tgccataacc gcttgctgcc
      121 aaggaaaaca aaatgaattt gcctattcaa aaattcatga tgctgtttgc agcggcaata
      181 tcgttgctgc aaatccccat tagtcatgcg aacggtttgg atgcccgttt gcgcgatgat
      241 atgcaggcaa aacactacga accgggtggc aaataccatc tgttcggtaa tggtcgcggc
      301 agtgttaaaa atcgggtttg cgccgtccaa acatttgatg caactgcggt cggccccata
      361 ctgcctatta cacacgaacg gacagggttt gaaggcatta tcggttatga aacccatttt
      421 tcaggacacg gacacgaagt acacagtccg ttcgataatc atgattcaaa a
//
LOCUS       DAXDZG010000179          460 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXDZG010000179 DAXDZG010000000
VERSION     DAXDZG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.179"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..209
                     /locus_tag="ACFB78_002151"
     CDS             <1..209
                     /locus_tag="ACFB78_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270039.1"
                     /translation="ILTKARRMYSWWAKESISLSGVARHGIRQDGSQICGELDSVWLE
                     AIEIIPVTGGAAESIRTALEVAQS"
     gene            206..445
                     /locus_tag="ACFB78_002152"
     CDS             206..445
                     /locus_tag="ACFB78_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270040.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGDGYGNGYGNGYGNGYGGVDGGGS
                     GSGYGNGKGDGDGSGNGDGDGNGNGSGNGSGNGDG"
BASE COUNT          113 a          132 c          156 g           59 t
ORIGIN      
        1 tgattctgac aaaagcgcgc cgaatgtaca gctggtgggc aaaggaatca atcagcctgt
       61 caggtgtcgc acgacacggc atcaggcaag acggcagcca aatttgcggc gagcttgatt
      121 ccgtatggct cgaggcgatt gagattatcc cagtaacagg cggcgcggct gaatcaatcc
      181 gcaccgcgct ggaggtcgcc cagtcatgag ttatctagat caaccattga agcacggcta
      241 cggcaacggc aacggtagcg gcaacggcga cggctatggc aacggctacg gcaacggcta
      301 cggcaacggc tacggcggcg tcgacggcgg cggcagcggc agcggctacg gcaacggcaa
      361 gggcgacggc gacggcagcg gcaacggcga cggcgacggc aacggcaacg gtagcggcaa
      421 cggtagcggc aacggcgacg gctaaacctt aaaaccgccc
//
LOCUS       DAXDZG010000180          431 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXDZG010000180 DAXDZG010000000
VERSION     DAXDZG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.180"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            136..351
                     /gene="rpmE"
                     /locus_tag="ACFB78_002153"
     CDS             136..351
                     /gene="rpmE"
                     /locus_tag="ACFB78_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC1270041.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          132 a          110 c           78 g          111 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatacat tttatcgaat atcctcttga cgaaacaaca
       61 gaaaatagcg agaatgccga gcttgtctga ggcataggta catcccctat gcccgctttt
      121 ttgaaaggaa atcaaatgaa acaaggtatt cacccgaact accgcgaagt taacgttacc
      181 tgctcttgcg gcaacaaatt cgtaaccaaa tccgcaatgg aaaaagaaaa cttcaacatc
      241 gaggtttgct ccctgtgcca cccgttctat accggcaccc aaaaaatcgt cgataccacc
      301 ggccgcgtgg acaaattcaa caacaaattc ggcaacctgt tcaaacgctg attgccgctt
      361 tgcaagaatt aagccctgcc atcatttcga tggcagggct ttttaatatt tggcacactg
      421 acttcttatc t
//
LOCUS       DAXDZG010000181          419 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXDZG010000181 DAXDZG010000000
VERSION     DAXDZG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.181"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT          101 a          122 c          127 g           69 t
ORIGIN      
        1 caaaaaccgc tgtaccatgg ataagcgcgc aagggtagaa taatgcggca accctataca
       61 ttgcaccccg tcagaggggc gcgttacctt tgcgaacatc cccctttggc agccgggcga
      121 agggggcttt gcaaccggca atccggcggg cgcgggatcg ggcggtttgc cgaatcccgc
      181 cgtttgcggc gcgcctgccg ccgacggtat cccgcgaagc aagatttaag ggataaaata
      241 tgttccaaca cacagggcgg cacataaggc gccgccctga ttcggaaggg cttgcacccc
      301 tcccggacaa agcctgatcc tgccgccccg aaggacggat gcccgaaggg cggggggttt
      361 gaccgaaaag gaaatacgat gaataaaact ttaaaaaggc gggttttccg ccataccgc
//
LOCUS       DAXDZG010000182          407 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXDZG010000182 DAXDZG010000000
VERSION     DAXDZG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.182"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..97
                     /locus_tag="ACFB78_002154"
                     /pseudo
     CDS             1..97
                     /locus_tag="ACFB78_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
     gene            137..407
                     /locus_tag="ACFB78_002155"
                     /pseudo
     CDS             137..407
                     /locus_tag="ACFB78_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
BASE COUNT          125 a          126 c           96 g           60 t
ORIGIN      
        1 cgacaacgcc gccaacgacg acgtcgccaa agacgacgcc gccgacaaaa tcgacaccaa
       61 gcacctgccg tcaacctgcc gcgataaatc atctgccgtt tgcacgaaac accacgcgcc
      121 gatttcaaac acttccaaaa aatcagccgt tgccgggtat tacccgaatc acggcaaatg
      181 gccgaaagac aacacttctg ccggcgtggc atccccccct ccgacatcaa aggcaaatat
      241 gttcaaagcg ttacggtcgc aaacggcgtc gttaccgccg aaatgaaatc agacggcgta
      301 aacaaagaaa tcaaaggcaa aaaactctcc ctgtggggca ggcgtcaaga cggttcggta
      361 aaatggttct gcggacagcc ggttaagcgc gacgacggca aagccaa
//
LOCUS       DAXDZG010000183          403 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXDZG010000183 DAXDZG010000000
VERSION     DAXDZG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.183"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>403
                     /locus_tag="ACFB78_002156"
     CDS             <1..>403
                     /locus_tag="ACFB78_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1270042.1"
                     /translation="GKWIKAEDLKAGSRLFAESGKTQTVRNIIVKPTPLKAYNLTVAD
                     WHTYFVKGNQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAKKLGYRKIKERTRND
                     AAIFKKGKSYISRDVDSHNGGAWKEASSPKNL"
BASE COUNT          145 a           82 c           96 g           80 t
ORIGIN      
        1 cggcaaatgg attaaagcgg aagatttgaa agcgggaagc aggctgtttg ccgaaagcgg
       61 caaaacccaa accgtccgca acatcattgt caaaccaaca ccgctcaaag cctacaatct
      121 gaccgttgcc gactggcata cctacttcgt caagggtaat caggcggaaa cggaaggggt
      181 ttgggttcat aatgcgtgtc cgccaagaaa aacaccatct acccccgtat atgggaatga
      241 ttcagaagca tatgcagcag caaagaaatt gggatataga aaaataaaag aaagaacgag
      301 aaatgatgct gctattttta aaaaaggaaa gagttatata tcacgcgatg tagattcaca
      361 taatggaggg gcttggaaag aagcatcttc acccaaaaat ctg
//
LOCUS       DAXDZG010000184          396 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXDZG010000184 DAXDZG010000000
VERSION     DAXDZG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.184"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..219
                     /locus_tag="ACFB78_002157"
     CDS             <1..219
                     /locus_tag="ACFB78_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270043.1"
                     /translation="FDLSDPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQ
                     AQAAEGRTEHPQGNAAPAEDIEDDIPFN"
BASE COUNT          116 a          120 c          112 g           48 t
ORIGIN      
        1 ttcgacctgt cagaccccga ttgggcgaat tacggcctcc tgaacgagaa actgagggag
       61 cagattgcca aaagcccgga atatgccgaa gccgtaaacg gccgccaacc gccggcgccg
      121 ccgcagaaac aggcccaagc ggcggaaggg cggacggaac acccccaagg caatgccgcg
      181 cctgccgaag acatcgagga cgacatccct tttaattaag cccgccgtca gggcgtaaaa
      241 ccggacggaa aaccgcaaac aaacgccgcg ccctgcgggc gggccccgca ctctgaagca
      301 gtagtcgatt tttagtttgc aactgcttcg gacggcaaca gagaaaggaa acaaaaaaat
      361 ggcaaacatc gacctgaccc aatgggacgg gaaaac
//
LOCUS       DAXDZG010000185          393 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXDZG010000185 DAXDZG010000000
VERSION     DAXDZG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.185"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>393
                     /locus_tag="ACFB78_002158"
     CDS             <1..>393
                     /locus_tag="ACFB78_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270044.1"
                     /translation="IKELLRNKGNGNRTDQKTENQENGTFHAVSSLGLSAVYDFDTGS
                     RFKPYIGARVAYGHVRHSIDSTKKTTKILTSFYGVATKPTTYDIGPKTQDAHQESNSI
                     RRVGLGVIAGVGFDITPKLTLDTGYRYH"
BASE COUNT          112 a          119 c           85 g           77 t
ORIGIN      
        1 cataaaagag ttgctaagaa acaagggcaa tggcaacagg acagaccaaa agacggaaaa
       61 tcaggaaaac ggtacgttcc acgccgtttc ttctctcggc ttgtccgccg tttacgattt
      121 cgataccggt tcccgcttca aaccctatat cggtgcgcgc gtcgcctacg gacacgtcag
      181 acacagcatc gattcgacca aaaaaacaac aaagattctt acctccttct atggtgttgc
      241 cacaaaacct acaacttatg atattggtcc aaagacgcaa gacgcccatc aagaaagcaa
      301 cagcatccgc cgcgtgggtc tcggcgtcat cgccggcgtc ggtttcgaca tcacgcccaa
      361 gctgaccctg gacaccggct accgctacca cta
//
LOCUS       DAXDZG010000186          390 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXDZG010000186 DAXDZG010000000
VERSION     DAXDZG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.186"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT          130 a           89 c           63 g          108 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaggtctt ttctaaaata tgcagatatt cgaatgtcga
       61 atttgcctta tgattacggt tttctgtctt atccgcctta aaacgccggt attccgtttg
      121 aaccaaatta tatttgccga aacactcaaa aatctccctt acctgcccga cagacatcaa
      181 gacttcgttg ttgtaactga gaaaaatata ttgaaagtcc gcatccctaa tcaaagcctc
      241 aaataccgga accacatccg attttgatca agtccgcgct aaaacacatc gtacagcgca
      301 aaaccggatg ggaatggcaa aatccggaaa caaacatttt tcaaagcgta caaacaaaaa
      361 ataccaaccg gaggttggta tttcttaact
//
LOCUS       DAXDZG010000187          384 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXDZG010000187 DAXDZG010000000
VERSION     DAXDZG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.187"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            160..>384
                     /locus_tag="ACFB78_002159"
     CDS             160..>384
                     /locus_tag="ACFB78_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270045.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSA"
BASE COUNT          134 a           89 c           86 g           75 t
ORIGIN      
        1 tggtgaaatc tgccgcgacg cagcagcgac atggaccact agaaaaagcg aaagcatcaa
       61 tcacaccgcc ttcactttta cagtgaaaaa agacggctta aaagagaagg taatgattgt
      121 tttgtaaaga aaccaacccc accccgaaag gaaacaaaaa tggaagcaaa taaatttgaa
      181 gtgaaaagtt tgtcagacct tataaaagtc tttgcaggca ttgctgccga tttcgaagcg
      241 gcaatgggtg taaaacgcgc cgacatttca accgaatttg acgaaccgca acatgagccg
      301 caaccgccgg taacagttgc cgagcaaaaa ggtatcaacg actttgccat cggcaaggaa
      361 gtcatcatcc gcacttattc ggca
//
LOCUS       DAXDZG010000188          380 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXDZG010000188 DAXDZG010000000
VERSION     DAXDZG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.188"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            101..379
                     /locus_tag="ACFB78_002160"
     CDS             101..379
                     /locus_tag="ACFB78_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC1270046.1"
                     /translation="MKTRNIAFKFAVLAAVLAAGYAFGFAKGGGSRTAKGKPAGIAAM
                     RMALAQKQAEVAELSAEIWLEERHLNADEEEAGCRRVHGDAEVPGGKE"
BASE COUNT           98 a           81 c          131 g           70 t
ORIGIN      
        1 ttgtagattt tagccgcaaa taaccccgac ggcgcacggt ccgtaccatc cttagcggac
       61 gcggtgcgca attaaaacct tttaaattaa ggaaatagaa atgaagacaa ggaatattgc
      121 gtttaagttc gcggtattgg cggcggtatt ggcggcgggc tatgccttcg gattcgccaa
      181 gggaggcgga agccgtacgg caaagggaaa acccgccggt attgcggcaa tgcgcatggc
      241 gttggctcaa aagcaggcgg aagttgcgga attgagcgcc gagatctggc tggaagagcg
      301 gcacctgaat gccgatgaag aagaagccgg ttgccggcgg gtgcacggcg atgcggaagt
      361 gccggggggt aaagaatgag
//
LOCUS       DAXDZG010000189          374 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXDZG010000189 DAXDZG010000000
VERSION     DAXDZG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.189"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(1..317)
                     /locus_tag="ACFB78_002161"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACFB78_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           70 a          106 c           93 g          105 t
ORIGIN      
        1 gccttacgca ccgcagacct tccgccgaag cagcggcttt tgaatttggt ttccccgctc
       61 tccctcgggt gcggggcaat gccgaccaaa cccgctatcc gtttgtgcga cagccgcccc
      121 aattcgggca gcatcgccat cagcgtagcc gtcgttatcg aaccgatgcc tttgatttgc
      181 tccgccactt gggctttgcc gtcaaaatgc gtgcgggtgt ggttgtcgat ttgtttgtcc
      241 gattcgtcaa tcagccggtc aaaacgggca atcagttgtt tgacgctttc gacttgcgtt
      301 tcgtgaacct gatgcagaca ttttgtaata gctgccatta cgcctactag gatatatacc
      361 ttctatatat agtg
//
LOCUS       DAXDZG010000190          370 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXDZG010000190 DAXDZG010000000
VERSION     DAXDZG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.190"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..>370)
                     /locus_tag="ACFB78_002162"
     CDS             complement(<1..>370)
                     /locus_tag="ACFB78_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270047.1"
                     /translation="YRFKFNPLYMAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNII
                     LCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANF
                     LKLGNPYNEDKLSLSEEHDTI"
BASE COUNT          127 a           54 c           49 g          140 t
ORIGIN      
        1 aatagtgtca tgttcttcac taagagataa tttatcttca ttatatggat taccgagttt
       61 taaaaaattt gctgcttttt ctatagcaac ttgccaagtt ttatatttat cgtaattttt
      121 atcaaaacgt actccccctt gtttattgga taaaaaagta attatctgat tcatattaaa
      181 aatatttcca ttatggaaaa ctcgttttaa agttaaaaat ttttcagcag gacataaaat
      241 tatatttgtt tcagcataga ttattggttc accagaaaat tcttgtgatg aatgatatat
      301 agggctaata atttttccgc ctaaataaac tccaccagcc atatagagtg gattaaattt
      361 aaaccggtac
//
LOCUS       DAXDZG010000191          367 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXDZG010000191 DAXDZG010000000
VERSION     DAXDZG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.191"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>367
                     /locus_tag="ACFB78_002163"
     CDS             <1..>367
                     /locus_tag="ACFB78_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC1270048.1"
                     /translation="RRPVDATGRGMRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAY
                     RETVRRIAQGAGAMPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVALDALQGVA
                     YHNDRQVRRIAADYADEPVA"
BASE COUNT           74 a          106 c          130 g           57 t
ORIGIN      
        1 ggcgccggcc tgtggacgca accggcaggg gaatgcgcgt gatacgcctt atcctgcctt
       61 accccgtatc ggcaaaccga tattggcgga tttggcgcaa cagggcggtc aggagcgcgg
      121 aggcggcggc gtatagggaa accgtccgcc gtatcgcgca aggggcgggc gcgatgccgt
      181 ccgaaggcgc ggttgccgta tatgtgcggc tgattcccaa agcgaacaaa gacggcggcg
      241 caaacaagac ggtgatcgat ttggacaacg ccctgaaggt tgcgctggac gcgcttcaag
      301 gcgttgccta tcacaacgac aggcaggtgc ggcgcattgc cgccgattac gccgacgagc
      361 cggtcgc
//
LOCUS       DAXDZG010000192          365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXDZG010000192 DAXDZG010000000
VERSION     DAXDZG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.192"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..253
                     /locus_tag="ACFB78_002164"
     CDS             <1..253
                     /locus_tag="ACFB78_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC1270049.1"
                     /translation="LLKMLIVRIILATGLTFVTYAGYLAALEKFKGYTANAINSMPSD
                     ILNLLLISGFGQGLGCLFGAFSFFIGMHAFKKLTFVFPG"
     gene            263..365
                     /locus_tag="ACFB78_002165"
                     /pseudo
     CDS             263..365
                     /locus_tag="ACFB78_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
BASE COUNT           94 a           82 c           86 g          103 t
ORIGIN      
        1 acttctgaaa atgctgattg tcagaatcat ccttgcaaca ggtctgacat tcgtaaccta
       61 tgccgggtat ctcgccgcac tggaaaagtt caaaggctac acggcaaatg cgatcaattc
      121 catgccttcc gacatattga accttctttt aatttcggga ttcggtcagg ggttgggctg
      181 cctgttcggc gcattctcgt tcttcattgg tatgcacgca ttcaaaaaac tgacgtttgt
      241 ctttccggga tgaggtagaa acatgattta tctgtttacg ggaaacatgg gggcaggcaa
      301 aacccccccg cgtcgtctct atgattttga acaacgaaga cggattgttc aaaatggaat
      361 tggaa
//
LOCUS       DAXDZG010000193          365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXDZG010000193 DAXDZG010000000
VERSION     DAXDZG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.193"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..364
                     /locus_tag="ACFB78_002166"
     CDS             <1..364
                     /locus_tag="ACFB78_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1270050.1"
                     /translation="EGQKSAVTEYYLNHGEWPEDNTSAGVASASDIKGKYVQKVEVNN
                     GVVTATMNSSNVNKEIKGKRLSLWARRENGSVKWFCGQPVKRTAAKADTVTDVAAANE
                     KIDTKHLPSTCRDEHDAK"
BASE COUNT          115 a          104 c           88 g           58 t
ORIGIN      
        1 cgaaggtcaa aaatcagccg ttaccgagta ttacctgaat cacggcgaat ggccggaaga
       61 caacacttct gccggcgtgg catccgcctc cgacatcaaa ggcaaatatg ttcagaaagt
      121 tgaagtcaac aacggcgtcg ttaccgccac aatgaattca agcaacgtaa acaaagaaat
      181 caaaggcaaa agactctccc tgtgggccag gcgtgaaaac ggttcggtaa aatggttctg
      241 cggacagccg gttaagcgca ccgccgccaa agccgacacc gttaccgacg ttgccgccgc
      301 caacgaaaaa atcgacacca agcacctgcc gtcaacctgc cgcgatgaac atgatgccaa
      361 atgag
//
LOCUS       DAXDZG010000194          361 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXDZG010000194 DAXDZG010000000
VERSION     DAXDZG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.194"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>361
                     /locus_tag="ACFB78_002167"
     CDS             <1..>361
                     /locus_tag="ACFB78_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1270051.1"
                     /translation="AILLAEGQKSAVTEYYLNHGIWPKDNTSAGVASSSSIKGKYVKE
                     VKVENGVVTATMNSSGVNKEIQGKRLSLWGRRENGSVKWFCGQPVTRAKADADADAAG
                     KDTTNIDTKHLPSTCRDA"
BASE COUNT          108 a          106 c           88 g           59 t
ORIGIN      
        1 agccatcctt ttggccgaag gtcaaaaatc agccgttacc gagtattacc tgaatcacgg
       61 catatggccg aaagacaaca cttctgccgg cgtggcatct tcttcatcaa tcaaaggcaa
      121 atatgttaag gaagttaaag tcgaaaacgg cgtcgtcacc gccacaatga attcaagcgg
      181 cgtaaacaaa gaaatccaag gcaaaagact ctccctgtgg ggcaggcgtg aaaacggttc
      241 ggtaaaatgg ttctgcggac agccggttac gcgcgccaaa gccgacgccg acgccgacgc
      301 cgccggcaaa gacaccacca acatcgacac caagcacctg ccgtcaacct gccgcgacgc
      361 a
//
LOCUS       DAXDZG010000195          354 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXDZG010000195 DAXDZG010000000
VERSION     DAXDZG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.195"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>354
                     /locus_tag="ACFB78_002168"
     CDS             <1..>354
                     /locus_tag="ACFB78_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270052.1"
                     /translation="NKNQNKRDLKTENQENGSFHAVSSLGLSAVYDFKLNDKFKPYIG
                     ARVAYGHVRHSIDSTKKTTRFLTSSYGGAKPTVYPEENTQDAHHQSNSIRRVGLGVIA
                     GVGFGITPKLTLDAG"
BASE COUNT          102 a          106 c           77 g           69 t
ORIGIN      
        1 aaacaagaat cagaataaga gagacctgaa gacggaaaat caggaaaacg gcagcttcca
       61 cgccgtttct tctctcggct tatcagccgt ttacgatttc aaactcaacg acaaattcaa
      121 accctatatc ggtgcgcgcg tcgcctacgg acacgtcaga cacagcatcg attcgaccaa
      181 aaaaacaaca cggtttctta cctcctccta tggtggcgca aaacctacgg tttatcctga
      241 ggaaaatacg caagacgccc atcaccaaag caacagcatc cgccgcgtag gtctcggtgt
      301 catcgccggc gtcggtttcg gcatcacgcc caagctgacc ttggacgccg gcta
//
LOCUS       DAXDZG010000196          323 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXDZG010000196 DAXDZG010000000
VERSION     DAXDZG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.196"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..>323)
                     /locus_tag="ACFB78_002169"
     CDS             complement(<1..>323)
                     /locus_tag="ACFB78_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270053.1"
                     /translation="RLGLYCNTDDPANGESVLFACREASQAVAQWTKDFDDLSPNQRQ
                     LVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLAVRVAKKAMAFLELDGGLISAVGKV
                     VNGADS"
BASE COUNT           67 a           97 c           91 g           68 t
ORIGIN      
        1 gaatccgcgc cgttgacgac tttgccgacc gccgaaatca aaccgccgtc aagttctaaa
       61 aacgccatgg ctttcttggc aacccgcacg gccaatgagt atgccgatac ttcggctatc
      121 agccgtgcag gcgcgtcttt cagaaattct tcgtacgcgg cgaagagatt tgacagcgga
      181 cgcagcacga gttggcgctg attcgggcta aggtcgtcga aatccttagt ccattgcgcg
      241 accgcctgcg atgcttcgcg gcaggcaaac agtacgcttt ccccgttcgc ggggtcgtcc
      301 gtgttgcagt acaggcccaa gcg
//
LOCUS       DAXDZG010000197          314 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXDZG010000197 DAXDZG010000000
VERSION     DAXDZG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.197"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            138..>314
                     /locus_tag="ACFB78_002170"
     CDS             138..>314
                     /locus_tag="ACFB78_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC1270054.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVG"
BASE COUNT           92 a           70 c           86 g           66 t
ORIGIN      
        1 aagttaccgt atcggcaaac gcgactgaaa aagacagcgt gttcggcgat gcggtgcaca
       61 ttgtgattgc gacttaaaac atagaacgag ttgccaaccc aaagtttaca tctcgtcttt
      121 taccgaaagg attaaaaatg acagaattac cgtggatagc cgaagcgaga aggcacatcg
      181 gtttgaaaga aattcccggc gcgaaacaca atccgacgat tgtgcaatgg ctcaaagaga
      241 cgggcggctt ccccggcgcg gcaaagtctt ggtattttga agacgaaacg ccgtggtgcg
      301 gcctgtttgt cgga
//
LOCUS       DAXDZG010000198          294 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXDZG010000198 DAXDZG010000000
VERSION     DAXDZG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.198"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>294
                     /locus_tag="ACFB78_002171"
     CDS             <1..>294
                     /locus_tag="ACFB78_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270055.1"
                     /translation="KYSVSIKELERNDNSTSSSNHLNIKTQKTEHQENGTFHAASSLG
                     LSTIYDFDTGSRFKPYIGARVAYGHVRHQVRSVEQETEIVTTYPKKGVPSPIPG"
BASE COUNT           95 a           81 c           54 g           64 t
ORIGIN      
        1 taaatattcc gtcagcataa aagagttgga aagaaacgat aatagcactt ctagcagcaa
       61 ccatcttaac ataaaaaccc aaaagacgga acatcaagaa aacggcacat tccacgccgc
      121 ctcttctctc ggcttgtcaa ccatttacga tttcgatacc ggttcccgct tcaaacccta
      181 tatcggcgca cgcgtcgcct acggacacgt cagacatcag gttcgttcgg ttgaacaaga
      241 aactgagatt gttaccactt acccaaaaaa aggtgtgcca agtcctatcc cagg
//
LOCUS       DAXDZG010000199          292 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXDZG010000199 DAXDZG010000000
VERSION     DAXDZG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.199"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(<1..>292)
                     /locus_tag="ACFB78_002172"
     CDS             complement(<1..>292)
                     /locus_tag="ACFB78_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC1270056.1"
                     /translation="AVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRGGGHVFFVVG
                     KDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAEGRYR"
BASE COUNT           58 a           92 c           87 g           55 t
ORIGIN      
        1 cggtaacgcc cttcggcggg ggacgaaggc acaggtttgc cgccaatcag cttggacggc
       61 cagaagtagc cgtcaatgtc cgcagggtca aacgggatga tggataccat attgccctga
      121 ttgccgccca agcccaagat tctgccttcc gcgtctttgc cgacaacgaa gaacacgtgt
      181 ccgccgcccc ggcgcggttt gaccgcgatg cagccgtatg cgggggcttc gagtttcgtc
      241 aaacccgaca ttgaccaggc tttggcgcga taccagtccc tgatgaccgc gc
//
LOCUS       DAXDZG010000200          289 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXDZG010000200 DAXDZG010000000
VERSION     DAXDZG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.200"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..185
                     /locus_tag="ACFB78_002173"
     CDS             <1..185
                     /locus_tag="ACFB78_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270057.1"
                     /translation="RARRGRGVMMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAES
                     EIKNYKAMLKTYERPD"
BASE COUNT           77 a           67 c           91 g           54 t
ORIGIN      
        1 ggcgcgcaag gcgcgggaga ggcgtaatga tgaatcctac cgagtttctg aaagcgcgta
       61 tcgccgaatg ggaagccaaa agcaaagagg cgggcggaaa cgccgatttt aaagcttttg
      121 agtttgccga aagcgaaatc aaaaactaca aagcgatgtt gaaaacttat gaacggcctg
      181 attaaaaacc cgaagctgat tgcggtgtcg gtttgcgccg tgttcgttgc cggcgcgtgg
      241 caatacgacc gcgccgcgca ataccggcgc ggatacggtg cggctatgc
//
LOCUS       DAXDZG010000201          286 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXDZG010000201 DAXDZG010000000
VERSION     DAXDZG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.201"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..204
                     /locus_tag="ACFB78_002174"
     CDS             <1..204
                     /locus_tag="ACFB78_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270058.1"
                     /translation="NFRFLFLVFCFRGNNGILSFRNLSEKQKSHRRHPRESGNLETKN
                     YRDLPETTNPLRRHSRAGGNLEI"
BASE COUNT           83 a           65 c           66 g           72 t
ORIGIN      
        1 aattttaggt ttctgttttt ggttttctgt tttcgcggga ataacggaat tttaagtttt
       61 aggaatttgt cggaaaaaca gaaatcccac cgccgtcatc cccgcgaaag cgggaatctg
      121 gaaacgaaaa actacaggga tttacccgaa acaacaaacc ctctccgccg tcattcccgc
      181 gcaggcggga atctagaaat ttaacgttgc ggtgatttat cggaaatgac tgaaacccaa
      241 cgaaccggat tcccactttc gcgggaatga cgaagtggaa gttacc
//
LOCUS       DAXDZG010000202          281 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXDZG010000202 DAXDZG010000000
VERSION     DAXDZG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.202"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>281
                     /locus_tag="ACFB78_002175"
     CDS             <1..>281
                     /locus_tag="ACFB78_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC1270059.1"
                     /translation="DLPHEVKDIHRPMMWSSNDPLPDDDYVFVPYLKESCFKGGAGAY
                     EIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIAEDAAIAV"
BASE COUNT           75 a           90 c           73 g           43 t
ORIGIN      
        1 gacctgcccc acgaagtcaa agacatccac cgcccgatga tgtggagcag caacgacccg
       61 ctgcccgacg atgattatgt tttcgtcccc tacctcaaag agagctgctt caaaggcggg
      121 gcgggcgcgt atgaaatccc cgactacaac ggctaccgcc tgccgttcgg caaatcaacg
      181 ttaaggcgca aaggcatcaa ccccgacaac gtcttctgct gcaccctgac cggcgacagc
      241 atggaggaaa aaatcgcaga agacgcggca atcgccgtag a
//
LOCUS       DAXDZG010000203          273 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXDZG010000203 DAXDZG010000000
VERSION     DAXDZG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.203"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>273
                     /locus_tag="ACFB78_002176"
     CDS             <1..>273
                     /locus_tag="ACFB78_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270060.1"
                     /translation="RNDNSTSSSRGHLNIQTRKTEHQENGTFHAVSSLGLSAVYDFKL
                     NDKFKPYIGARVAYGHVRHQVRSVEQETVAVTTYPKEQNVAPSVTT"
BASE COUNT           84 a           76 c           55 g           58 t
ORIGIN      
        1 aagaaacgat aatagcactt ctagcagcag aggccatctt aacatacaaa cccgaaagac
       61 ggaacatcag gaaaacggca cattccacgc cgtttcttct ctcggcttgt ccgccgttta
      121 cgatttcaaa ctcaacgata aattcaaacc ctatatcggc gcgcgcgtcg cctacggaca
      181 cgtcagacat caggttcgtt cggttgaaca agaaaccgtt gctgttacca cttacccaaa
      241 ggaacagaat gttgcgccaa gtgttaccac aga
//
LOCUS       DAXDZG010000204          272 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXDZG010000204 DAXDZG010000000
VERSION     DAXDZG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.204"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>272
                     /locus_tag="ACFB78_002177"
     CDS             <1..>272
                     /locus_tag="ACFB78_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1270061.1"
                     /translation="GYYLNHGKWPENNDKAGVASPATDIKGKYVKEVKVENGVVTATM
                     KSDGVNKEIKGKRLSLWAKREAGSVKWFCGRPVKRTAGNAKDGNDD"
BASE COUNT           87 a           75 c           74 g           36 t
ORIGIN      
        1 ccgggtatta cctgaatcac ggcaaatggc cggaaaacaa cgacaaggcc ggcgtggcat
       61 cccccgccac cgacatcaaa ggcaaatatg ttaaggaagt taaagtcgaa aacggcgtcg
      121 tcaccgccac aatgaaatca gacggcgtaa acaaagaaat caaaggcaaa agactctccc
      181 tgtgggccaa gcgtgaagcc ggttcggtaa aatggttctg cggacggccg gttaagcgca
      241 ccgccggcaa cgccaaagac ggcaacgacg ac
//
LOCUS       DAXDZG010000205          272 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXDZG010000205 DAXDZG010000000
VERSION     DAXDZG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.205"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>272
                     /locus_tag="ACFB78_002178"
     CDS             <1..>272
                     /locus_tag="ACFB78_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC1270062.1"
                     /translation="YQLHRTGSEIHPADGYDGPQGGGYPEPQGARDIYSYHIKGTSTK
                     TKINTVPQAPFSDRWLKENAGAASGFLSRADEAGKLIWENDPDKNW"
BASE COUNT           87 a           66 c           66 g           53 t
ORIGIN      
        1 tttaccaact tcatcggaca gggtcggaaa tacatcccgc agacggatat gacgggcctc
       61 aaggcggcgg ttatccggaa ccacaagggg caagggatat atacagttac catatcaaag
      121 gaacttctac aaaaacaaag ataaacactg ttccgcaagc ccctttttca gaccgctggc
      181 taaaagaaaa tgccggtgcc gcttccggtt ttctcagccg tgcggatgaa gcaggaaaac
      241 tgatatggga aaacgacccc gataaaaatt gg
//
LOCUS       DAXDZG010000206          270 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXDZG010000206 DAXDZG010000000
VERSION     DAXDZG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.206"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            complement(17..>270)
                     /locus_tag="ACFB78_002179"
     CDS             complement(17..>270)
                     /locus_tag="ACFB78_002179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270063.1"
                     /translation="HAQGSGGMRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVF
                     QRGEDGKPQRVSRIRKRSATPGQARERTKTHDVSRTQQP"
BASE COUNT           29 a           77 c           87 g           77 t
ORIGIN      
        1 gtagccgccg gcttttttac ggttgttgtg tccttgaaac gtcatgggtt ttcgtccgtt
       61 ctctggcttg tccgggtgtg gcggagcgtt tgcggatgcg gctgacgcgc tgcggtttgc
      121 cgtcttcgcc gcgctgaaaa acgcttgttc cgccgcgccg gcagccgtgc cgcttatgcc
      181 tgatgccgtg tttgaagcct tttgtgccgg aatcgtgata cacgcaggtc cggatgtgcg
      241 tcctcatgcc gccgcttccc tgtgcatgag
//
LOCUS       DAXDZG010000207          259 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXDZG010000207 DAXDZG010000000
VERSION     DAXDZG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.207"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..199
                     /locus_tag="ACFB78_002180"
     CDS             <1..199
                     /locus_tag="ACFB78_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270064.1"
                     /translation="AAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVY
                     RNACFDADGVRELNAAVDDGG"
BASE COUNT           62 a           78 c           79 g           40 t
ORIGIN      
        1 agccgccgcg gtcgaacacg ccgaacacgc ccgcaaatcg tccgccgcgt atcaggcgca
       61 aaaggcggcg cgtgaggaaa aagaaagggt gcgctatgtg caaacgctta aaatcattga
      121 aaaacctgtg taccgcaatg cctgttttga tgctgacggc gtgcgcgaac tcaacgccgc
      181 cgttgacgac ggcggttaag ccgcccgccg atttggtgcg gccctgcccg aaactgccgc
      241 accttgaagg gaacacggg
//
LOCUS       DAXDZG010000208          253 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXDZG010000208 DAXDZG010000000
VERSION     DAXDZG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.208"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>253
                     /locus_tag="ACFB78_002181"
     CDS             <1..>253
                     /locus_tag="ACFB78_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270065.1"
                     /translation="ANTAVAQYSGHKKTAGGKSVPIGCMEKGGKTVCPNGYVYDKPVN
                     VRYLDETETDGFSTTADGDAGGSGIDPAGRRPGKNNRCFS"
BASE COUNT           67 a           69 c           73 g           44 t
ORIGIN      
        1 gccaatacgg ctgtcgcaca atattccggc cataagaaaa ccgccggcgg caagtccgtc
       61 cctataggtt gtatggaaaa aggcggtaaa accgtctgcc cgaacggata tgtttacgac
      121 aagccggtta atgtgcgtta tctggatgaa acggaaacag acggattttc aacgacggcg
      181 gacggcgatg cgggcggcag cggtatagac cccgccggca ggcgtcccgg caaaaacaac
      241 cgctgcttct cca
//
LOCUS       DAXDZG010000209          250 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXDZG010000209 DAXDZG010000000
VERSION     DAXDZG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.209"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>250
                     /locus_tag="ACFB78_002182"
     CDS             <1..>250
                     /locus_tag="ACFB78_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1270066.1"
                     /translation="HDSVAQYSGHKKTADGKSVPIGCMWKNGKTACPNGYVYDKPVNV
                     RYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFS"
BASE COUNT           73 a           69 c           66 g           42 t
ORIGIN      
        1 cacgattcgg ttgcgcaata ttccggccat aagaaaaccg ccgacggcaa gtccgtccct
       61 ataggctgta tgtggaaaaa cggcaaaacc gcctgcccga acggatatgt ttacgacaag
      121 ccggttaatg tgcgctacct ggacgaaaag aaaacagacg atttccccgt cacggcagac
      181 ggtgatgcag gcggcagcgg aacattcaaa gagggtaaaa aacccgcccg caataaccgg
      241 tgcttctccg
//
LOCUS       DAXDZG010000210          240 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXDZG010000210 DAXDZG010000000
VERSION     DAXDZG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.210"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>240
                     /locus_tag="ACFB78_002183"
     CDS             <1..>240
                     /locus_tag="ACFB78_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC1270067.1"
                     /translation="EMDAKKPEEILSLKVDADPDKYIKATGYPGYSEKVEVAPGTKVN
                     MGPVTDRNGNPVQVAATFGRDAQGNTTADVQVIPRP"
BASE COUNT           76 a           61 c           65 g           38 t
ORIGIN      
        1 gaaatggatg ccaaaaagcc ggaagagatt ttatcgttga aagtcgatgc cgatcccgac
       61 aaatacataa aggcaaccgg atatcccggt tattccgaaa aagtagaagt cgcacccgga
      121 acaaaagtga atatggggcc cgtcacggac aggaacggga atcccgttca ggttgccgca
      181 acattcggca gggacgcgca aggcaacacc acggcggatg tacaagtaat cccgcgtccc
//
LOCUS       DAXDZG010000211          239 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXDZG010000211 DAXDZG010000000
VERSION     DAXDZG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.211"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            1..239
                     /locus_tag="ACFB78_002184"
                     /pseudo
     CDS             1..239
                     /locus_tag="ACFB78_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776296.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           49 a           68 c           70 g           52 t
ORIGIN      
        1 gcaatgggac atcggcggca gcggggcggt tttcccttcg ctcgcactgt ttctgctctg
       61 tttcatcata ggtatgcaca acacggggat gacgcttctg ccgggcggtg caatctgttc
      121 gacgcacatg gcccggcacg gcagccgact tgggcatcga aatcccgcgc gtgccgtact
      181 atagtggatt aacaaaaacc agtacggcgt tggcccgcct tagctcaaag agaacgatt
//
LOCUS       DAXDZG010000212          236 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXDZG010000212 DAXDZG010000000
VERSION     DAXDZG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.212"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..>236
                     /locus_tag="ACFB78_002185"
     CDS             <1..>236
                     /locus_tag="ACFB78_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1270068.1"
                     /translation="KLNDKFKPYIGARVAYGHVRHSIDSTKKTIEFLTAPSNAPNGAS
                     TNYDISPKTQNAHQESNSIRRVGLGVIAGVGFDI"
BASE COUNT           71 a           72 c           46 g           47 t
ORIGIN      
        1 tcaaactcaa cgataaattc aaaccctata tcggtgcgcg cgtcgcctac ggacacgtca
       61 gacacagcat cgattcgacc aaaaaaacaa tagagtttct taccgccccc tccaatgctc
      121 ctaacggagc atctacaaat tatgatatta gtccaaagac gcaaaacgcc catcaagaaa
      181 gcaacagcat ccgccgcgtg ggtctcggtg tcatcgccgg cgtcggtttc gacatc
//
LOCUS       DAXDZG010000213          233 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXDZG010000213 DAXDZG010000000
VERSION     DAXDZG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.213"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT           64 a           54 c           54 g           61 t
ORIGIN      
        1 atatcggttt ccgttcggac aaatgtctgc gcaggaataa atggccgcgt ttgaattctc
       61 ggttaattcc atttattagc cgcttgcttc tgtttgcatg aaagccggga ttattgaata
      121 aaatacattt gcacggccgg ttaggtttgc ccgccggaat attaaaacac cgcccaaaaa
      181 aatcagccgt tgccgggtat tgcccgaatc acggcaaatg gccggaaaac aac
//
LOCUS       DAXDZG010000214          233 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXDZG010000214 DAXDZG010000000
VERSION     DAXDZG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.214"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT           56 a           76 c           51 g           50 t
ORIGIN      
        1 tcgtcattcc cgcgcaggcg ggaatctaga tctgtcagtg caggaactta tcggataaaa
       61 cggttgccca aaccccgcgt cctagattcc cacttccgtg ggaatgacgg ttcggttgcg
      121 ttccgacaac accgtagtct taaaacccgt cattcccgac aacaccgtaa tcttgaaacc
      181 cgtcattccc gacaataccg caatctcgaa acccgtcatt cccgcgcagg cgg
//
LOCUS       DAXDZG010000215          219 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXDZG010000215 DAXDZG010000000
VERSION     DAXDZG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.215"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
BASE COUNT           59 a           52 c           64 g           44 t
ORIGIN      
        1 ggaccggtcg ggcatctgcg gcggtttgct aaaaaacgct ttaccgtgat aagtgcgcaa
       61 agttaaaatg gggaggtaag cttttcaatc agcaatccgg cgggcgcgga atcgggcggt
      121 ttaccgaacc ccggcgttcg cggcgcgccg tcccgcgaag gcaaacttaa ggaataaaat
      181 atgaataaaa ctttaaaaag gcgggttttc cgccatacc
//
LOCUS       DAXDZG010000216          206 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG078
            SAMN08583246-rid23188793.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXDZG010000216 DAXDZG010000000
VERSION     DAXDZG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583246
            Sequence Read Archive: SRR6765450
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362592.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 11:52:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,138
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 118 of 176
            Pseudo Genes (incomplete)         :: 64 of 176
            Pseudo Genes (internal stop)      :: 40 of 176
            Pseudo Genes (multiple problems)  :: 44 of 176
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583246-rid23188793.denovo.216"
                     /strain="NG078"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Tianjin"
                     /lat_lon="39.02 N 117.12 E"
                     /collection_date="2012-05-25"
                     /collected_by="NCSTD"
     gene            <1..140
                     /locus_tag="ACFB78_002186"
     CDS             <1..140
                     /locus_tag="ACFB78_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC1270069.1"
                     /translation="ANAALPETAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVM
                     K"
BASE COUNT           55 a           42 c           61 g           48 t
ORIGIN      
        1 aggcaaacgc agcgttgccc gaaacggcaa aaaacgcttt ggaagccgca aaagcggacg
       61 gtatggaagc cggttggatt gtagtgggcg ttttcgccgc gctttttgta ttttccatcg
      121 ttaagagggt gatgaagtaa ggcggcatgt actgccaagt cggaaataaa tgtcttgaga
      181 agcaccgggc cgaaaacctt tatttc
//
